---
_id: '15084'
abstract:
- lang: eng
text: "GABAB receptor (GBR) activation inhibits neurotransmitter release in axon
terminals in the brain, except in medial habenula (MHb) terminals, which show
robust potentiation. However, mechanisms underlying this enigmatic potentiation
remain elusive. Here, we report that GBR activation on MHb terminals induces an
activity-dependent transition from a facilitating, tonic to a depressing, phasic
neurotransmitter release mode. This transition is accompanied by a 4.1-fold increase
in readily releasable vesicle pool (RRP) size and a 3.5-fold increase of docked
synaptic vesicles (SVs) at the presynaptic active zone (AZ). Strikingly, the depressing
phasic release exhibits looser coupling distance than the tonic release. Furthermore,
the tonic and phasic release are selectively affected by deletion of synaptoporin
(SPO) and Ca\r\n 2+\r\n -dependent
activator protein for secretion 2 (CAPS2), respectively. SPO modulates augmentation,
the short-term plasticity associated with tonic release, and CAPS2 retains the
increased RRP for initial responses in phasic response trains. The cytosolic protein
CAPS2 showed a SV-associated distribution similar to the vesicular transmembrane
protein SPO, and they were colocalized in the same terminals. We developed the
“Flash and Freeze-fracture” method, and revealed the release of SPO-associated
vesicles in both tonic and phasic modes and activity-dependent recruitment of
CAPS2 to the AZ during phasic release, which lasted several minutes. Overall,
these results indicate that GBR activation translocates CAPS2 to the AZ along
with the fusion of CAPS2-associated SVs, contributing to persistency of the RRP
increase. Thus, we identified structural and molecular mechanisms underlying tonic
and phasic neurotransmitter release and their transition by GBR activation in
MHb terminals."
acknowledged_ssus:
- _id: M-Shop
- _id: PreCl
- _id: EM-Fac
acknowledgement: We thank Erwin Neher and Ipe Ninan for critical comments on the manuscript.
This project has received funding from the European Research Council (ERC) and European
Commission, under the European Union’s Horizon 2020 research and innovation program
(ERC grant agreement no. 694539 to R.S. and the Marie Skłodowska-Curie grant agreement
no. 665385 to C.Ö.). This study was supported by the Cooperative Study Program of
Center for Animal Resources and Collaborative Study of NINS. We thank Kohgaku Eguchi
for statistical analysis, Yu Kasugai for additional EM imaging, Robert Beattie for
the design of the slice recovery chamber for Flash and Freeze experiments, Todor
Asenov from the ISTA machine shop for custom part preparations for high-pressure
freezing, the ISTA preclinical facility for animal caretaking, and the ISTA EM facilities
for technical support.
article_number: e2301449121
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Peter
full_name: Koppensteiner, Peter
id: 3B8B25A8-F248-11E8-B48F-1D18A9856A87
last_name: Koppensteiner
orcid: 0000-0002-3509-1948
- first_name: Pradeep
full_name: Bhandari, Pradeep
id: 45EDD1BC-F248-11E8-B48F-1D18A9856A87
last_name: Bhandari
orcid: 0000-0003-0863-4481
- first_name: Hüseyin C
full_name: Önal, Hüseyin C
id: 4659D740-F248-11E8-B48F-1D18A9856A87
last_name: Önal
orcid: 0000-0002-2771-2011
- first_name: Carolina
full_name: Borges Merjane, Carolina
id: 4305C450-F248-11E8-B48F-1D18A9856A87
last_name: Borges Merjane
orcid: 0000-0003-0005-401X
- first_name: Elodie
full_name: Le Monnier, Elodie
id: 3B59276A-F248-11E8-B48F-1D18A9856A87
last_name: Le Monnier
- first_name: Utsa
full_name: Roy, Utsa
id: 4d26cf11-5355-11ee-ae5a-eb05e255b9b2
last_name: Roy
- first_name: Yukihiro
full_name: Nakamura, Yukihiro
last_name: Nakamura
- first_name: Tetsushi
full_name: Sadakata, Tetsushi
last_name: Sadakata
- first_name: Makoto
full_name: Sanbo, Makoto
last_name: Sanbo
- first_name: Masumi
full_name: Hirabayashi, Masumi
last_name: Hirabayashi
- first_name: JeongSeop
full_name: Rhee, JeongSeop
last_name: Rhee
- first_name: Nils
full_name: Brose, Nils
last_name: Brose
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
citation:
ama: Koppensteiner P, Bhandari P, Önal C, et al. GABAB receptors induce phasic release
from medial habenula terminals through activity-dependent recruitment of release-ready
vesicles. Proceedings of the National Academy of Sciences. 2024;121(8).
doi:10.1073/pnas.2301449121
apa: Koppensteiner, P., Bhandari, P., Önal, C., Borges Merjane, C., Le Monnier,
E., Roy, U., … Shigemoto, R. (2024). GABAB receptors induce phasic release from
medial habenula terminals through activity-dependent recruitment of release-ready
vesicles. Proceedings of the National Academy of Sciences. Proceedings
of the National Academy of Sciences. https://doi.org/10.1073/pnas.2301449121
chicago: Koppensteiner, Peter, Pradeep Bhandari, Cihan Önal, Carolina Borges Merjane,
Elodie Le Monnier, Utsa Roy, Yukihiro Nakamura, et al. “GABAB Receptors Induce
Phasic Release from Medial Habenula Terminals through Activity-Dependent Recruitment
of Release-Ready Vesicles.” Proceedings of the National Academy of Sciences.
Proceedings of the National Academy of Sciences, 2024. https://doi.org/10.1073/pnas.2301449121.
ieee: P. Koppensteiner et al., “GABAB receptors induce phasic release from
medial habenula terminals through activity-dependent recruitment of release-ready
vesicles,” Proceedings of the National Academy of Sciences, vol. 121, no.
8. Proceedings of the National Academy of Sciences, 2024.
ista: Koppensteiner P, Bhandari P, Önal C, Borges Merjane C, Le Monnier E, Roy U,
Nakamura Y, Sadakata T, Sanbo M, Hirabayashi M, Rhee J, Brose N, Jonas PM, Shigemoto
R. 2024. GABAB receptors induce phasic release from medial habenula terminals
through activity-dependent recruitment of release-ready vesicles. Proceedings
of the National Academy of Sciences. 121(8), e2301449121.
mla: Koppensteiner, Peter, et al. “GABAB Receptors Induce Phasic Release from Medial
Habenula Terminals through Activity-Dependent Recruitment of Release-Ready Vesicles.”
Proceedings of the National Academy of Sciences, vol. 121, no. 8, e2301449121,
Proceedings of the National Academy of Sciences, 2024, doi:10.1073/pnas.2301449121.
short: P. Koppensteiner, P. Bhandari, C. Önal, C. Borges Merjane, E. Le Monnier,
U. Roy, Y. Nakamura, T. Sadakata, M. Sanbo, M. Hirabayashi, J. Rhee, N. Brose,
P.M. Jonas, R. Shigemoto, Proceedings of the National Academy of Sciences 121
(2024).
date_created: 2024-03-05T09:23:55Z
date_published: 2024-02-20T00:00:00Z
date_updated: 2024-03-12T13:44:18Z
day: '20'
ddc:
- '570'
department:
- _id: RySh
- _id: PeJo
doi: 10.1073/pnas.2301449121
ec_funded: 1
external_id:
pmid:
- '38346189'
file:
- access_level: open_access
checksum: b25b2a057c266ff317a48b0d54d6fc8a
content_type: application/pdf
creator: dernst
date_created: 2024-03-12T13:42:42Z
date_updated: 2024-03-12T13:42:42Z
file_id: '15110'
file_name: 2024_PNAS_Koppensteiner.pdf
file_size: 13648221
relation: main_file
success: 1
file_date_updated: 2024-03-12T13:42:42Z
has_accepted_license: '1'
intvolume: ' 121'
issue: '8'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 25CA28EA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '694539'
name: 'In situ analysis of single channel subunit composition in neurons: physiological
implication in synaptic plasticity and behaviour'
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
publication: Proceedings of the National Academy of Sciences
publication_identifier:
eissn:
- 1091-6490
issn:
- 0027-8424
publication_status: published
publisher: Proceedings of the National Academy of Sciences
quality_controlled: '1'
related_material:
link:
- description: News on ISTA Website
relation: press_release
url: https://ista.ac.at/en/news/neuronal-insights-flash-and-freeze-fracture/
record:
- id: '13173'
relation: research_data
status: public
status: public
title: GABAB receptors induce phasic release from medial habenula terminals through
activity-dependent recruitment of release-ready vesicles
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 121
year: '2024'
...
---
_id: '14843'
abstract:
- lang: eng
text: The coupling between Ca2+ channels and release sensors is a key factor defining
the signaling properties of a synapse. However, the coupling nanotopography at
many synapses remains unknown, and it is unclear how it changes during development.
To address these questions, we examined coupling at the cerebellar inhibitory
basket cell (BC)-Purkinje cell (PC) synapse. Biophysical analysis of transmission
by paired recording and intracellular pipette perfusion revealed that the effects
of exogenous Ca2+ chelators decreased during development, despite constant reliance
of release on P/Q-type Ca2+ channels. Structural analysis by freeze-fracture replica
labeling (FRL) and transmission electron microscopy (EM) indicated that presynaptic
P/Q-type Ca2+ channels formed nanoclusters throughout development, whereas docked
vesicles were only clustered at later developmental stages. Modeling suggested
a developmental transformation from a more random to a more clustered coupling
nanotopography. Thus, presynaptic signaling developmentally approaches a point-to-point
configuration, optimizing speed, reliability, and energy efficiency of synaptic
transmission.
acknowledged_ssus:
- _id: EM-Fac
- _id: PreCl
- _id: M-Shop
acknowledgement: We thank Drs. David DiGregorio and Erwin Neher for critically reading
an earlier version of the manuscript, Ralf Schneggenburger for helpful discussions,
Benjamin Suter and Katharina Lichter for support with image analysis, Chris Wojtan
for advice on numerical solution of partial differential equations, Maria Reva for
help with Ripley analysis, Alois Schlögl for programming, and Akari Hagiwara and
Toshihisa Ohtsuka for anti-ELKS antibody. We are grateful to Florian Marr, Christina
Altmutter, and Vanessa Zheden for excellent technical assistance and to Eleftheria
Kralli-Beller for manuscript editing. This research was supported by the Scientific
Services Units (SSUs) of ISTA (Electron Microscopy Facility, Preclinical Facility,
and Machine Shop). The project received funding from the European Research Council
(ERC) under the European Union’s Horizon 2020 research and innovation program (grant
agreement no. 692692), the Fonds zur Förderung der Wissenschaftlichen Forschung
(Z 312-B27, Wittgenstein award; P 36232-B), all to P.J., and a DOC fellowship of
the Austrian Academy of Sciences to J.-J.C.
article_processing_charge: No
article_type: original
author:
- first_name: JingJing
full_name: Chen, JingJing
id: 2C4E65C8-F248-11E8-B48F-1D18A9856A87
last_name: Chen
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Chong
full_name: Chen, Chong
id: 3DFD581A-F248-11E8-B48F-1D18A9856A87
last_name: Chen
- first_name: Itaru
full_name: Arai, Itaru
id: 32A73F6C-F248-11E8-B48F-1D18A9856A87
last_name: Arai
- first_name: Olena
full_name: Kim, Olena
id: 3F8ABDDA-F248-11E8-B48F-1D18A9856A87
last_name: Kim
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Chen J, Kaufmann W, Chen C, et al. Developmental transformation of Ca2+ channel-vesicle
nanotopography at a central GABAergic synapse. Neuron. doi:10.1016/j.neuron.2023.12.002
apa: Chen, J., Kaufmann, W., Chen, C., Arai, itaru, Kim, O., Shigemoto, R., &
Jonas, P. M. (n.d.). Developmental transformation of Ca2+ channel-vesicle nanotopography
at a central GABAergic synapse. Neuron. Elsevier. https://doi.org/10.1016/j.neuron.2023.12.002
chicago: Chen, JingJing, Walter Kaufmann, Chong Chen, itaru Arai, Olena Kim, Ryuichi
Shigemoto, and Peter M Jonas. “Developmental Transformation of Ca2+ Channel-Vesicle
Nanotopography at a Central GABAergic Synapse.” Neuron. Elsevier, n.d.
https://doi.org/10.1016/j.neuron.2023.12.002.
ieee: J. Chen et al., “Developmental transformation of Ca2+ channel-vesicle
nanotopography at a central GABAergic synapse,” Neuron. Elsevier.
ista: Chen J, Kaufmann W, Chen C, Arai itaru, Kim O, Shigemoto R, Jonas PM. Developmental
transformation of Ca2+ channel-vesicle nanotopography at a central GABAergic synapse.
Neuron.
mla: Chen, JingJing, et al. “Developmental Transformation of Ca2+ Channel-Vesicle
Nanotopography at a Central GABAergic Synapse.” Neuron, Elsevier, doi:10.1016/j.neuron.2023.12.002.
short: J. Chen, W. Kaufmann, C. Chen, itaru Arai, O. Kim, R. Shigemoto, P.M. Jonas,
Neuron (n.d.).
date_created: 2024-01-21T23:00:56Z
date_published: 2024-01-11T00:00:00Z
date_updated: 2024-03-14T13:14:18Z
day: '11'
department:
- _id: PeJo
- _id: EM-Fac
- _id: RySh
doi: 10.1016/j.neuron.2023.12.002
ec_funded: 1
external_id:
pmid:
- '38215739'
language:
- iso: eng
month: '01'
oa_version: None
pmid: 1
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: The Wittgenstein Prize
- _id: bd88be38-d553-11ed-ba76-81d5a70a6ef5
grant_number: P36232
name: Mechanisms of GABA release in hippocampal circuits
- _id: 26B66A3E-B435-11E9-9278-68D0E5697425
grant_number: '25383'
name: Development of nanodomain coupling between Ca2+ channels and release sensors
at a central inhibitory synapse
publication: Neuron
publication_identifier:
eissn:
- 1097-4199
issn:
- 0896-6273
publication_status: inpress
publisher: Elsevier
quality_controlled: '1'
related_material:
link:
- description: News on ISTA Website
relation: press_release
url: https://ista.ac.at/en/news/synapses-brought-to-the-point/
record:
- id: '15101'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Developmental transformation of Ca2+ channel-vesicle nanotopography at a central
GABAergic synapse
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2024'
...
---
_id: '15101'
acknowledged_ssus:
- _id: EM-Fac
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: JingJing
full_name: Chen, JingJing
id: 2C4E65C8-F248-11E8-B48F-1D18A9856A87
last_name: Chen
citation:
ama: Chen J. Developmental transformation of nanodomain coupling between Ca2+ channels
and release sensors at a central GABAergic synapse. 2024. doi:10.15479/at:ista:15101
apa: Chen, J. (2024). Developmental transformation of nanodomain coupling between
Ca2+ channels and release sensors at a central GABAergic synapse. Institute
of Science and Technology Austria. https://doi.org/10.15479/at:ista:15101
chicago: Chen, JingJing. “Developmental Transformation of Nanodomain Coupling between
Ca2+ Channels and Release Sensors at a Central GABAergic Synapse.” Institute of
Science and Technology Austria, 2024. https://doi.org/10.15479/at:ista:15101.
ieee: J. Chen, “Developmental transformation of nanodomain coupling between Ca2+
channels and release sensors at a central GABAergic synapse,” Institute of Science
and Technology Austria, 2024.
ista: Chen J. 2024. Developmental transformation of nanodomain coupling between
Ca2+ channels and release sensors at a central GABAergic synapse. Institute of
Science and Technology Austria.
mla: Chen, JingJing. Developmental Transformation of Nanodomain Coupling between
Ca2+ Channels and Release Sensors at a Central GABAergic Synapse. Institute
of Science and Technology Austria, 2024, doi:10.15479/at:ista:15101.
short: J. Chen, Developmental Transformation of Nanodomain Coupling between Ca2+
Channels and Release Sensors at a Central GABAergic Synapse, Institute of Science
and Technology Austria, 2024.
date_created: 2024-03-11T10:09:54Z
date_published: 2024-03-11T00:00:00Z
date_updated: 2024-03-14T13:14:19Z
day: '11'
ddc:
- '570'
degree_awarded: PhD
department:
- _id: GradSch
- _id: PeJo
doi: 10.15479/at:ista:15101
ec_funded: 1
file:
- access_level: closed
checksum: db4947474ffa271e66c254b6fe876a55
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: jchen
date_created: 2024-03-11T14:10:58Z
date_updated: 2024-03-12T07:12:17Z
file_id: '15104'
file_name: Thesis_Jingjing CHEN.docx
file_size: 11271363
relation: source_file
- access_level: closed
checksum: a5eeae8b5702cd540f5d03469bc33dde
content_type: application/pdf
creator: jchen
date_created: 2024-03-11T14:11:06Z
date_updated: 2024-03-11T14:11:06Z
embargo: 2024-04-01
embargo_to: open_access
file_id: '15105'
file_name: Thesis_Jingjing CHEN_merged.pdf
file_size: 16627311
relation: main_file
file_date_updated: 2024-03-12T07:12:17Z
has_accepted_license: '1'
language:
- iso: eng
month: '03'
oa_version: Published Version
page: '84'
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: The Wittgenstein Prize
- _id: bd88be38-d553-11ed-ba76-81d5a70a6ef5
grant_number: P36232
name: Mechanisms of GABA release in hippocampal circuits
- _id: 26B66A3E-B435-11E9-9278-68D0E5697425
grant_number: '25383'
name: Development of nanodomain coupling between Ca2+ channels and release sensors
at a central inhibitory synapse
publication_identifier:
issn:
- 2663 - 337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '14843'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
title: Developmental transformation of nanodomain coupling between Ca2+ channels and
release sensors at a central GABAergic synapse
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: dissertation
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
year: '2024'
...
---
_id: '15117'
abstract:
- lang: eng
text: 'The hippocampal mossy fiber synapse, formed between axons of dentate gyrus
granule cells and dendrites of CA3 pyramidal neurons, is a key synapse in the
trisynaptic circuitry of the hippocampus. Because of its comparatively large size,
this synapse is accessible to direct presynaptic recording, allowing a rigorous
investigation of the biophysical mechanisms of synaptic transmission and plasticity.
Furthermore, because of its placement in the very center of the hippocampal memory
circuit, this synapse seems to be critically involved in several higher network
functions, such as learning, memory, pattern separation, and pattern completion.
Recent work based on new technologies in both nanoanatomy and nanophysiology,
including presynaptic patch-clamp recording, paired recording, super-resolution
light microscopy, and freeze-fracture and “flash-and-freeze” electron microscopy,
has provided new insights into the structure, biophysics, and network function
of this intriguing synapse. This brings us one step closer to answering a fundamental
question in neuroscience: how basic synaptic properties shape higher network computations.'
acknowledgement: "We thank previous students, postdocs, and collaborators, particularly
J. Geiger, and (in alphabetical order) H. Alle, J. Bischofberger, C. Borges-Merjane,
D. Engel, M. Frotscher, S. Hallermann, M. Heckmann, S. Jamrichova, O. Kim, L. Li,
K. Lichter, P. Lin, J. Lübke, Y. Okamoto, C. Pawlu, C. Schmidt-Hieber, N. Spruston,
and N. Vyleta for their outstanding experimental contributions. We also thank P.
Castillo, J. Geiger, T. Sakaba, S. Siegert, T. Vogels, and J. Watson for critically
reading the manuscript, E. Kralli-Beller for text editing, and J. Malikovic and
L. Slomianka for useful discussions. We apologize that, due to space constraints,
not all relevant papers could be cited.\r\nThis project was supported by the European
Research Council (ERC) under the European Union’s Horizon 2020 research and innovation
program (grant agreement 692692, AdG “GIANTSYN”) and the Fonds zur Förderung der
Wissenschaftlichen Forschung (Z 312-B27, Wittgenstein Award; P 36232-B, stand-alone
grant), both to P.J."
article_processing_charge: No
article_type: review
author:
- first_name: David H
full_name: Vandael, David H
id: 3AE48E0A-F248-11E8-B48F-1D18A9856A87
last_name: Vandael
orcid: 0000-0001-7577-1676
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Vandael DH, Jonas PM. Structure, biophysics, and circuit function of a “giant”
cortical presynaptic terminal. Science. 2024;383(6687):eadg6757. doi:10.1126/science.adg6757
apa: Vandael, D. H., & Jonas, P. M. (2024). Structure, biophysics, and circuit
function of a “giant” cortical presynaptic terminal. Science. AAAS. https://doi.org/10.1126/science.adg6757
chicago: Vandael, David H, and Peter M Jonas. “Structure, Biophysics, and Circuit
Function of a ‘Giant’ Cortical Presynaptic Terminal.” Science. AAAS, 2024.
https://doi.org/10.1126/science.adg6757.
ieee: D. H. Vandael and P. M. Jonas, “Structure, biophysics, and circuit function
of a ‘giant’ cortical presynaptic terminal,” Science, vol. 383, no. 6687.
AAAS, p. eadg6757, 2024.
ista: Vandael DH, Jonas PM. 2024. Structure, biophysics, and circuit function of
a ‘giant’ cortical presynaptic terminal. Science. 383(6687), eadg6757.
mla: Vandael, David H., and Peter M. Jonas. “Structure, Biophysics, and Circuit
Function of a ‘Giant’ Cortical Presynaptic Terminal.” Science, vol. 383,
no. 6687, AAAS, 2024, p. eadg6757, doi:10.1126/science.adg6757.
short: D.H. Vandael, P.M. Jonas, Science 383 (2024) eadg6757.
date_created: 2024-03-17T23:00:57Z
date_published: 2024-03-08T00:00:00Z
date_updated: 2024-03-20T07:42:52Z
day: '08'
department:
- _id: PeJo
doi: 10.1126/science.adg6757
ec_funded: 1
external_id:
pmid:
- '38452088'
intvolume: ' 383'
issue: '6687'
language:
- iso: eng
month: '03'
oa_version: None
page: eadg6757
pmid: 1
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: The Wittgenstein Prize
- _id: bd88be38-d553-11ed-ba76-81d5a70a6ef5
grant_number: P36232
name: Mechanisms of GABA release in hippocampal circuits
publication: Science
publication_identifier:
eissn:
- 1095-9203
publication_status: published
publisher: AAAS
quality_controlled: '1'
scopus_import: '1'
status: public
title: Structure, biophysics, and circuit function of a "giant" cortical presynaptic
terminal
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 383
year: '2024'
...
---
_id: '12720'
abstract:
- lang: eng
text: Here we describe the in vivo DNA assembly approach, where molecular cloning
procedures are performed using an E. coli recA-independent recombination pathway,
which assembles linear fragments of DNA with short homologous termini. This pathway
is present in all standard laboratory E. coli strains and, by bypassing the need
for in vitro DNA assembly, allows simplified molecular cloning to be performed
without the plasmid instability issues associated with specialized recombination-cloning
bacterial strains. The methodology requires specific primer design and can perform
all standard plasmid modifications (insertions, deletions, mutagenesis, and sub-cloning)
in a rapid, simple, and cost-efficient manner, as it does not require commercial
kits or specialized bacterial strains. Additionally, this approach can be used
to perform complex procedures such as multiple modifications to a plasmid, as
up to 6 linear fragments can be assembled in vivo by this recombination pathway.
Procedures generally require less than 3 h, involving PCR amplification, DpnI
digestion of template DNA, and transformation, upon which circular plasmids are
assembled. In this chapter we describe the requirements, procedure, and potential
pitfalls when using this technique, as well as protocol variations to overcome
the most common issues.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Sandra
full_name: Arroyo-Urea, Sandra
last_name: Arroyo-Urea
- first_name: Jake
full_name: Watson, Jake
id: 63836096-4690-11EA-BD4E-32803DDC885E
last_name: Watson
orcid: 0000-0002-8698-3823
- first_name: Javier
full_name: García-Nafría, Javier
last_name: García-Nafría
citation:
ama: 'Arroyo-Urea S, Watson J, García-Nafría J. Molecular Cloning Using In Vivo
DNA Assembly. In: Scarlett G, ed. DNA Manipulation and Analysis. Vol 2633.
MIMB. New York, NY, United States: Springer Nature; 2023:33-44. doi:10.1007/978-1-0716-3004-4_3'
apa: 'Arroyo-Urea, S., Watson, J., & García-Nafría, J. (2023). Molecular Cloning
Using In Vivo DNA Assembly. In G. Scarlett (Ed.), DNA Manipulation and Analysis
(Vol. 2633, pp. 33–44). New York, NY, United States: Springer Nature. https://doi.org/10.1007/978-1-0716-3004-4_3'
chicago: 'Arroyo-Urea, Sandra, Jake Watson, and Javier García-Nafría. “Molecular
Cloning Using In Vivo DNA Assembly.” In DNA Manipulation and Analysis,
edited by Garry Scarlett, 2633:33–44. MIMB. New York, NY, United States: Springer
Nature, 2023. https://doi.org/10.1007/978-1-0716-3004-4_3.'
ieee: 'S. Arroyo-Urea, J. Watson, and J. García-Nafría, “Molecular Cloning Using
In Vivo DNA Assembly,” in DNA Manipulation and Analysis, vol. 2633, G.
Scarlett, Ed. New York, NY, United States: Springer Nature, 2023, pp. 33–44.'
ista: 'Arroyo-Urea S, Watson J, García-Nafría J. 2023.Molecular Cloning Using In
Vivo DNA Assembly. In: DNA Manipulation and Analysis. Methods in Molecular Biology,
vol. 2633, 33–44.'
mla: Arroyo-Urea, Sandra, et al. “Molecular Cloning Using In Vivo DNA Assembly.”
DNA Manipulation and Analysis, edited by Garry Scarlett, vol. 2633, Springer
Nature, 2023, pp. 33–44, doi:10.1007/978-1-0716-3004-4_3.
short: S. Arroyo-Urea, J. Watson, J. García-Nafría, in:, G. Scarlett (Ed.), DNA
Manipulation and Analysis, Springer Nature, New York, NY, United States, 2023,
pp. 33–44.
date_created: 2023-03-12T23:01:02Z
date_published: 2023-03-01T00:00:00Z
date_updated: 2023-03-16T08:34:24Z
day: '01'
department:
- _id: PeJo
doi: 10.1007/978-1-0716-3004-4_3
editor:
- first_name: Garry
full_name: Scarlett, Garry
last_name: Scarlett
external_id:
pmid:
- '36853454'
intvolume: ' 2633'
language:
- iso: eng
month: '03'
oa_version: None
page: 33-44
place: New York, NY, United States
pmid: 1
publication: DNA Manipulation and Analysis
publication_identifier:
eisbn:
- 978-1-0716-3004-4
eissn:
- 1940-6029
isbn:
- 978-1-0716-3003-7
issn:
- 1064-3745
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
series_title: MIMB
status: public
title: Molecular Cloning Using In Vivo DNA Assembly
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2633
year: '2023'
...
---
_id: '12567'
abstract:
- lang: eng
text: Single-molecule localization microscopy (SMLM) greatly advances structural
studies of diverse biological tissues. For example, presynaptic active zone (AZ)
nanotopology is resolved in increasing detail. Immunofluorescence imaging of AZ
proteins usually relies on epitope preservation using aldehyde-based immunocompetent
fixation. Cryofixation techniques, such as high-pressure freezing (HPF) and freeze
substitution (FS), are widely used for ultrastructural studies of presynaptic
architecture in electron microscopy (EM). HPF/FS demonstrated nearer-to-native
preservation of AZ ultrastructure, e.g., by facilitating single filamentous structures.
Here, we present a protocol combining the advantages of HPF/FS and direct stochastic
optical reconstruction microscopy (dSTORM) to quantify nanotopology of the AZ
scaffold protein Bruchpilot (Brp) at neuromuscular junctions (NMJs) of Drosophila
melanogaster. Using this standardized model, we tested for preservation of Brp
clusters in different FS protocols compared to classical aldehyde fixation. In
HPF/FS samples, presynaptic boutons were structurally well preserved with ~22%
smaller Brp clusters that allowed quantification of subcluster topology. In summary,
we established a standardized near-to-native preparation and immunohistochemistry
protocol for SMLM analyses of AZ protein clusters in a defined model synapse.
Our protocol could be adapted to study protein arrangements at single-molecule
resolution in other intact tissue preparations.
acknowledgement: This work has been supported by funding of the German Research Foundation
(Deutsche Forschungsgemeinschaft [DFG], CRC 166, Project B06 to M.H. and A.-L.S.,
FOR 3004 SYNABS P1 to M.H.) and by the Interdisciplinary Clinical Research Center
(IZKF) Würzburg (Z-3/69 to M.M.P., N-229 to M.H. and A.-L.S.). A.M. is funded by
the University of Leipzig Clinician Scientist Program.
article_number: '2128'
article_processing_charge: No
article_type: original
author:
- first_name: Achmed
full_name: Mrestani, Achmed
last_name: Mrestani
- first_name: Katharina
full_name: Lichter, Katharina
id: 39302e62-fcfc-11ec-8196-8b01447dbd3d
last_name: Lichter
- first_name: Anna Leena
full_name: Sirén, Anna Leena
last_name: Sirén
- first_name: Manfred
full_name: Heckmann, Manfred
last_name: Heckmann
- first_name: Mila M.
full_name: Paul, Mila M.
last_name: Paul
- first_name: Martin
full_name: Pauli, Martin
last_name: Pauli
citation:
ama: Mrestani A, Lichter K, Sirén AL, Heckmann M, Paul MM, Pauli M. Single-molecule
localization microscopy of presynaptic active zones in Drosophila melanogaster
after rapid cryofixation. International Journal of Molecular Sciences.
2023;24(3). doi:10.3390/ijms24032128
apa: Mrestani, A., Lichter, K., Sirén, A. L., Heckmann, M., Paul, M. M., & Pauli,
M. (2023). Single-molecule localization microscopy of presynaptic active zones
in Drosophila melanogaster after rapid cryofixation. International Journal
of Molecular Sciences. MDPI. https://doi.org/10.3390/ijms24032128
chicago: Mrestani, Achmed, Katharina Lichter, Anna Leena Sirén, Manfred Heckmann,
Mila M. Paul, and Martin Pauli. “Single-Molecule Localization Microscopy of Presynaptic
Active Zones in Drosophila Melanogaster after Rapid Cryofixation.” International
Journal of Molecular Sciences. MDPI, 2023. https://doi.org/10.3390/ijms24032128.
ieee: A. Mrestani, K. Lichter, A. L. Sirén, M. Heckmann, M. M. Paul, and M. Pauli,
“Single-molecule localization microscopy of presynaptic active zones in Drosophila
melanogaster after rapid cryofixation,” International Journal of Molecular
Sciences, vol. 24, no. 3. MDPI, 2023.
ista: Mrestani A, Lichter K, Sirén AL, Heckmann M, Paul MM, Pauli M. 2023. Single-molecule
localization microscopy of presynaptic active zones in Drosophila melanogaster
after rapid cryofixation. International Journal of Molecular Sciences. 24(3),
2128.
mla: Mrestani, Achmed, et al. “Single-Molecule Localization Microscopy of Presynaptic
Active Zones in Drosophila Melanogaster after Rapid Cryofixation.” International
Journal of Molecular Sciences, vol. 24, no. 3, 2128, MDPI, 2023, doi:10.3390/ijms24032128.
short: A. Mrestani, K. Lichter, A.L. Sirén, M. Heckmann, M.M. Paul, M. Pauli, International
Journal of Molecular Sciences 24 (2023).
date_created: 2023-02-19T23:00:56Z
date_published: 2023-01-21T00:00:00Z
date_updated: 2023-08-01T13:16:36Z
day: '21'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.3390/ijms24032128
external_id:
isi:
- '000930324700001'
file:
- access_level: open_access
checksum: 69a35dcd3e0249f902ab881b06ee2e58
content_type: application/pdf
creator: dernst
date_created: 2023-02-20T07:09:27Z
date_updated: 2023-02-20T07:09:27Z
file_id: '12569'
file_name: 2023_IJMS_Mrestani.pdf
file_size: 2823025
relation: main_file
success: 1
file_date_updated: 2023-02-20T07:09:27Z
has_accepted_license: '1'
intvolume: ' 24'
isi: 1
issue: '3'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
publication: International Journal of Molecular Sciences
publication_identifier:
eissn:
- 1422-0067
publication_status: published
publisher: MDPI
quality_controlled: '1'
scopus_import: '1'
status: public
title: Single-molecule localization microscopy of presynaptic active zones in Drosophila
melanogaster after rapid cryofixation
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 24
year: '2023'
...
---
_id: '12759'
abstract:
- lang: eng
text: Stereological methods for estimating the 3D particle size and density from
2D projections are essential to many research fields. These methods are, however,
prone to errors arising from undetected particle profiles due to sectioning and
limited resolution, known as ‘lost caps’. A potential solution developed by Keiding,
Jensen, and Ranek in 1972, which we refer to as the Keiding model, accounts for
lost caps by quantifying the smallest detectable profile in terms of its limiting
‘cap angle’ (ϕ), a size-independent measure of a particle’s distance from the
section surface. However, this simple solution has not been widely adopted nor
tested. Rather, model-independent design-based stereological methods, which do
not explicitly account for lost caps, have come to the fore. Here, we provide
the first experimental validation of the Keiding model by comparing the size and
density of particles estimated from 2D projections with direct measurement from
3D EM reconstructions of the same tissue. We applied the Keiding model to estimate
the size and density of somata, nuclei and vesicles in the cerebellum of mice
and rats, where high packing density can be problematic for design-based methods.
Our analysis reveals a Gaussian distribution for ϕ rather than a single value.
Nevertheless, curve fits of the Keiding model to the 2D diameter distribution
accurately estimate the mean ϕ and 3D diameter distribution. While systematic
testing using simulations revealed an upper limit to determining ϕ, our analysis
shows that estimated ϕ can be used to determine the 3D particle density from the
2D density under a wide range of conditions, and this method is potentially more
accurate than minimum-size-based lost-cap corrections and disector methods. Our
results show the Keiding model provides an efficient means of accurately estimating
the size and density of particles from 2D projections even under conditions of
a high density.
acknowledged_ssus:
- _id: EM-Fac
acknowledgement: "We thank the IST Austria Electron Microscopy Facility for technical
support, and Diccon Coyle, Andrea Lőrincz and Zoltan Nusser for their helpful comments
and discussions.\r\nFunding for JSR and RAS was from the Wellcome Trust (203048;
224499; https://\r\nwellcome.org/). RAS is in receipt of a Wellcome Trust Principal
Research Fellowship (224499).\r\nFunding for CBM and PJ was from Fond zur Förderung
der Wissenschaftlichen Forschung (V\r\n739-B27 Elise-Richter Programme to CBM, Z
312-B27 Wittgenstein Award to PJ; \r\nhttps://www.fwf.ac.at). PJ received funding
from the European Research Council (ERC; https://erc.europa.eu) under the European
Union’s Horizon 2020 research and innovation programme (grant agreement no. 692692).
NH was supported by a European\r\nResearch Council Advanced Grant (ERC-AG787157)."
article_number: e0277148
article_processing_charge: No
article_type: original
author:
- first_name: Jason Seth
full_name: Rothman, Jason Seth
last_name: Rothman
- first_name: Carolina
full_name: Borges Merjane, Carolina
id: 4305C450-F248-11E8-B48F-1D18A9856A87
last_name: Borges Merjane
orcid: 0000-0003-0005-401X
- first_name: Noemi
full_name: Holderith, Noemi
last_name: Holderith
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: R.
full_name: Angus Silver, R.
last_name: Angus Silver
citation:
ama: Rothman JS, Borges Merjane C, Holderith N, Jonas PM, Angus Silver R. Validation
of a stereological method for estimating particle size and density from 2D projections
with high accuracy. PLoS ONE. 2023;18(3 March). doi:10.1371/journal.pone.0277148
apa: Rothman, J. S., Borges Merjane, C., Holderith, N., Jonas, P. M., & Angus
Silver, R. (2023). Validation of a stereological method for estimating particle
size and density from 2D projections with high accuracy. PLoS ONE. Public
Library of Science. https://doi.org/10.1371/journal.pone.0277148
chicago: Rothman, Jason Seth, Carolina Borges Merjane, Noemi Holderith, Peter M
Jonas, and R. Angus Silver. “Validation of a Stereological Method for Estimating
Particle Size and Density from 2D Projections with High Accuracy.” PLoS ONE.
Public Library of Science, 2023. https://doi.org/10.1371/journal.pone.0277148.
ieee: J. S. Rothman, C. Borges Merjane, N. Holderith, P. M. Jonas, and R. Angus
Silver, “Validation of a stereological method for estimating particle size and
density from 2D projections with high accuracy,” PLoS ONE, vol. 18, no.
3 March. Public Library of Science, 2023.
ista: Rothman JS, Borges Merjane C, Holderith N, Jonas PM, Angus Silver R. 2023.
Validation of a stereological method for estimating particle size and density
from 2D projections with high accuracy. PLoS ONE. 18(3 March), e0277148.
mla: Rothman, Jason Seth, et al. “Validation of a Stereological Method for Estimating
Particle Size and Density from 2D Projections with High Accuracy.” PLoS ONE,
vol. 18, no. 3 March, e0277148, Public Library of Science, 2023, doi:10.1371/journal.pone.0277148.
short: J.S. Rothman, C. Borges Merjane, N. Holderith, P.M. Jonas, R. Angus Silver,
PLoS ONE 18 (2023).
date_created: 2023-03-26T22:01:07Z
date_published: 2023-03-17T00:00:00Z
date_updated: 2023-08-01T13:46:39Z
day: '17'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1371/journal.pone.0277148
ec_funded: 1
external_id:
isi:
- '001024737400001'
file:
- access_level: open_access
checksum: 2380331ec27cc87808826fc64419ac1c
content_type: application/pdf
creator: dernst
date_created: 2023-03-27T06:51:09Z
date_updated: 2023-03-27T06:51:09Z
file_id: '12770'
file_name: 2023_PLoSOne_Rothman.pdf
file_size: 7290413
relation: main_file
success: 1
file_date_updated: 2023-03-27T06:51:09Z
has_accepted_license: '1'
intvolume: ' 18'
isi: 1
issue: 3 March
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: The Wittgenstein Prize
- _id: 2696E7FE-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: V00739
name: Structural plasticity at mossy fiber-CA3 synapses
publication: PLoS ONE
publication_identifier:
eissn:
- 1932-6203
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
scopus_import: '1'
status: public
title: Validation of a stereological method for estimating particle size and density
from 2D projections with high accuracy
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 18
year: '2023'
...
---
_id: '12515'
abstract:
- lang: eng
text: "Introduction: The olfactory system in most mammals is divided into several
subsystems based on the anatomical locations of the neuroreceptor cells involved
and the receptor families that are expressed. In addition to the main olfactory
system and the vomeronasal system, a range of olfactory subsystems converge onto
the transition zone located between the main olfactory bulb (MOB) and the accessory
olfactory bulb (AOB), which has been termed the olfactory limbus (OL). The OL
contains specialized glomeruli that receive noncanonical sensory afferences and
which interact with the MOB and AOB. Little is known regarding the olfactory subsystems
of mammals other than laboratory rodents.\r\nMethods: We have focused on characterizing
the OL in the red fox by performing general and specific histological stainings
on serial sections, using both single and double immunohistochemical and lectin-histochemical
labeling techniques.\r\nResults: As a result, we have been able to determine that
the OL of the red fox (Vulpes vulpes) displays an uncommonly high degree of development
and complexity.\r\nDiscussion: This makes this species a novel mammalian model,
the study of which could improve our understanding of the noncanonical pathways
involved in the processing of chemosensory cues."
acknowledgement: This work was partially supported by a grant from “Consello Social
Universidade de Santiago de Compostela” 2022-PU004.We would like to show special
gratitude to Prof. Ludwig Wagner (Medical University, Vienna) for kindly providing
us with the secretagogin antibody. We thank the Wildlife Recovery Centres of Galicia,
Dirección Xeral de Patrimonio Natural (Xunta de Galicia, Spain), and Federación
Galega de Caza for providing the red foxes used in this study.
article_number: '1097467'
article_processing_charge: No
article_type: original
author:
- first_name: Irene
full_name: Ortiz-Leal, Irene
last_name: Ortiz-Leal
- first_name: Mateo V.
full_name: Torres, Mateo V.
last_name: Torres
- first_name: Victor M
full_name: Vargas Barroso, Victor M
id: 2F55A9DE-F248-11E8-B48F-1D18A9856A87
last_name: Vargas Barroso
- first_name: Luis Eusebio
full_name: Fidalgo, Luis Eusebio
last_name: Fidalgo
- first_name: Ana María
full_name: López-Beceiro, Ana María
last_name: López-Beceiro
- first_name: Jorge A.
full_name: Larriva-Sahd, Jorge A.
last_name: Larriva-Sahd
- first_name: Pablo
full_name: Sánchez-Quinteiro, Pablo
last_name: Sánchez-Quinteiro
citation:
ama: Ortiz-Leal I, Torres MV, Vargas Barroso VM, et al. The olfactory limbus of
the red fox (Vulpes vulpes). New insights regarding a noncanonical olfactory bulb
pathway. Frontiers in Neuroanatomy. 2023;16. doi:10.3389/fnana.2022.1097467
apa: Ortiz-Leal, I., Torres, M. V., Vargas Barroso, V. M., Fidalgo, L. E., López-Beceiro,
A. M., Larriva-Sahd, J. A., & Sánchez-Quinteiro, P. (2023). The olfactory
limbus of the red fox (Vulpes vulpes). New insights regarding a noncanonical olfactory
bulb pathway. Frontiers in Neuroanatomy. Frontiers. https://doi.org/10.3389/fnana.2022.1097467
chicago: Ortiz-Leal, Irene, Mateo V. Torres, Victor M Vargas Barroso, Luis Eusebio
Fidalgo, Ana María López-Beceiro, Jorge A. Larriva-Sahd, and Pablo Sánchez-Quinteiro.
“The Olfactory Limbus of the Red Fox (Vulpes Vulpes). New Insights Regarding a
Noncanonical Olfactory Bulb Pathway.” Frontiers in Neuroanatomy. Frontiers,
2023. https://doi.org/10.3389/fnana.2022.1097467.
ieee: I. Ortiz-Leal et al., “The olfactory limbus of the red fox (Vulpes
vulpes). New insights regarding a noncanonical olfactory bulb pathway,” Frontiers
in Neuroanatomy, vol. 16. Frontiers, 2023.
ista: Ortiz-Leal I, Torres MV, Vargas Barroso VM, Fidalgo LE, López-Beceiro AM,
Larriva-Sahd JA, Sánchez-Quinteiro P. 2023. The olfactory limbus of the red fox
(Vulpes vulpes). New insights regarding a noncanonical olfactory bulb pathway.
Frontiers in Neuroanatomy. 16, 1097467.
mla: Ortiz-Leal, Irene, et al. “The Olfactory Limbus of the Red Fox (Vulpes Vulpes).
New Insights Regarding a Noncanonical Olfactory Bulb Pathway.” Frontiers in
Neuroanatomy, vol. 16, 1097467, Frontiers, 2023, doi:10.3389/fnana.2022.1097467.
short: I. Ortiz-Leal, M.V. Torres, V.M. Vargas Barroso, L.E. Fidalgo, A.M. López-Beceiro,
J.A. Larriva-Sahd, P. Sánchez-Quinteiro, Frontiers in Neuroanatomy 16 (2023).
date_created: 2023-02-05T23:01:00Z
date_published: 2023-01-10T00:00:00Z
date_updated: 2023-08-16T11:37:52Z
day: '10'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.3389/fnana.2022.1097467
external_id:
isi:
- '000919786900001'
pmid:
- '36704406'
file:
- access_level: open_access
checksum: 49cd40f3bda6f267079427042e7d15e3
content_type: application/pdf
creator: dernst
date_created: 2023-02-06T07:56:14Z
date_updated: 2023-02-06T07:56:14Z
file_id: '12518'
file_name: 2022_FrontiersNeuroanatomy_OrtizLeal.pdf
file_size: 21943473
relation: main_file
success: 1
file_date_updated: 2023-02-06T07:56:14Z
has_accepted_license: '1'
intvolume: ' 16'
isi: 1
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
pmid: 1
publication: Frontiers in Neuroanatomy
publication_identifier:
eissn:
- 1662-5129
publication_status: published
publisher: Frontiers
quality_controlled: '1'
scopus_import: '1'
status: public
title: The olfactory limbus of the red fox (Vulpes vulpes). New insights regarding
a noncanonical olfactory bulb pathway
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 16
year: '2023'
...
---
_id: '12786'
abstract:
- lang: eng
text: AMPA glutamate receptors (AMPARs) mediate excitatory neurotransmission throughout
the brain. Their signalling is uniquely diversified by brain region-specific auxiliary
subunits, providing an opportunity for the development of selective therapeutics.
AMPARs associated with TARP γ8 are enriched in the hippocampus, and are targets
of emerging anti-epileptic drugs. To understand their therapeutic activity, we
determined cryo-EM structures of the GluA1/2-γ8 receptor associated with three
potent, chemically diverse ligands. We find that despite sharing a lipid-exposed
and water-accessible binding pocket, drug action is differentially affected by
binding-site mutants. Together with patch-clamp recordings and MD simulations
we also demonstrate that ligand-triggered reorganisation of the AMPAR-TARP interface
contributes to modulation. Unexpectedly, one ligand (JNJ-61432059) acts bifunctionally,
negatively affecting GluA1 but exerting positive modulatory action on GluA2-containing
AMPARs, in a TARP stoichiometry-dependent manner. These results further illuminate
the action of TARPs, demonstrate the sensitive balance between positive and negative
modulatory action, and provide a mechanistic platform for development of both
positive and negative selective AMPAR modulators.
acknowledgement: We thank James Krieger for generating the ‘proDy’ interaction maps
in Fig. 5B and S7C, and Jan-Niklas Dohrke for critically reading the manuscript.
We thank members of the Greger lab for insightful comments during this study. We
acknowledge Trevor Rutherford for confirming ligand integrity by NMR. We are also
grateful to LMB scientific computing and the EM facility for their support. This
research was funded in part by the Wellcome Trust (223194/Z/21/Z) to I.H.G. For
the purpose of Open Access, the MRC Laboratory of Molecular Biology has applied
a CC BY public copyright licence to any Author Accepted Manuscript (AAM) version
arising from this submission. Further funding came from the Medical Research Council
(MRU105174197) to I.H.G, and NIH grant (R56/R01MH123474) to T.N.
article_number: '1659'
article_processing_charge: No
article_type: original
author:
- first_name: Danyang
full_name: Zhang, Danyang
last_name: Zhang
- first_name: Remigijus
full_name: Lape, Remigijus
last_name: Lape
- first_name: Saher A.
full_name: Shaikh, Saher A.
last_name: Shaikh
- first_name: Bianka K.
full_name: Kohegyi, Bianka K.
last_name: Kohegyi
- first_name: Jake
full_name: Watson, Jake
id: 63836096-4690-11EA-BD4E-32803DDC885E
last_name: Watson
orcid: 0000-0002-8698-3823
- first_name: Ondrej
full_name: Cais, Ondrej
last_name: Cais
- first_name: Terunaga
full_name: Nakagawa, Terunaga
last_name: Nakagawa
- first_name: Ingo H.
full_name: Greger, Ingo H.
last_name: Greger
citation:
ama: Zhang D, Lape R, Shaikh SA, et al. Modulatory mechanisms of TARP γ8-selective
AMPA receptor therapeutics. Nature Communications. 2023;14. doi:10.1038/s41467-023-37259-5
apa: Zhang, D., Lape, R., Shaikh, S. A., Kohegyi, B. K., Watson, J., Cais, O., …
Greger, I. H. (2023). Modulatory mechanisms of TARP γ8-selective AMPA receptor
therapeutics. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-023-37259-5
chicago: Zhang, Danyang, Remigijus Lape, Saher A. Shaikh, Bianka K. Kohegyi, Jake
Watson, Ondrej Cais, Terunaga Nakagawa, and Ingo H. Greger. “Modulatory Mechanisms
of TARP Γ8-Selective AMPA Receptor Therapeutics.” Nature Communications.
Springer Nature, 2023. https://doi.org/10.1038/s41467-023-37259-5.
ieee: D. Zhang et al., “Modulatory mechanisms of TARP γ8-selective AMPA receptor
therapeutics,” Nature Communications, vol. 14. Springer Nature, 2023.
ista: Zhang D, Lape R, Shaikh SA, Kohegyi BK, Watson J, Cais O, Nakagawa T, Greger
IH. 2023. Modulatory mechanisms of TARP γ8-selective AMPA receptor therapeutics.
Nature Communications. 14, 1659.
mla: Zhang, Danyang, et al. “Modulatory Mechanisms of TARP Γ8-Selective AMPA Receptor
Therapeutics.” Nature Communications, vol. 14, 1659, Springer Nature, 2023,
doi:10.1038/s41467-023-37259-5.
short: D. Zhang, R. Lape, S.A. Shaikh, B.K. Kohegyi, J. Watson, O. Cais, T. Nakagawa,
I.H. Greger, Nature Communications 14 (2023).
date_created: 2023-04-02T22:01:09Z
date_published: 2023-03-25T00:00:00Z
date_updated: 2023-12-13T11:15:58Z
day: '25'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1038/s41467-023-37259-5
external_id:
isi:
- '001066658700003'
file:
- access_level: open_access
checksum: 0a97b31191432dae5853bbb5ccb7698d
content_type: application/pdf
creator: dernst
date_created: 2023-04-03T06:38:56Z
date_updated: 2023-04-03T06:38:56Z
file_id: '12797'
file_name: 2023_NatureComm_Zhang.pdf
file_size: 2613996
relation: main_file
success: 1
file_date_updated: 2023-04-03T06:38:56Z
has_accepted_license: '1'
intvolume: ' 14'
isi: 1
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
publication: Nature Communications
publication_identifier:
eissn:
- 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Modulatory mechanisms of TARP γ8-selective AMPA receptor therapeutics
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 14
year: '2023'
...
---
_id: '13267'
abstract:
- lang: eng
text: Three-dimensional (3D) reconstruction of living brain tissue down to an individual
synapse level would create opportunities for decoding the dynamics and structure–function
relationships of the brain’s complex and dense information processing network;
however, this has been hindered by insufficient 3D resolution, inadequate signal-to-noise
ratio and prohibitive light burden in optical imaging, whereas electron microscopy
is inherently static. Here we solved these challenges by developing an integrated
optical/machine-learning technology, LIONESS (live information-optimized nanoscopy
enabling saturated segmentation). This leverages optical modifications to stimulated
emission depletion microscopy in comprehensively, extracellularly labeled tissue
and previous information on sample structure via machine learning to simultaneously
achieve isotropic super-resolution, high signal-to-noise ratio and compatibility
with living tissue. This allows dense deep-learning-based instance segmentation
and 3D reconstruction at a synapse level, incorporating molecular, activity and
morphodynamic information. LIONESS opens up avenues for studying the dynamic functional
(nano-)architecture of living brain tissue.
acknowledged_ssus:
- _id: ScienComp
- _id: Bio
- _id: PreCl
- _id: E-Lib
- _id: LifeSc
- _id: M-Shop
acknowledgement: "We thank J. Vorlaufer, N. Agudelo and A. Wartak for microscope maintenance
and troubleshooting, C. Kreuzinger and A. Freeman for technical assistance, M. Šuplata
for hardware control support and M. Cunha dos Santos for initial exploration of
software. We\r\nthank P. Henderson for advice on deep-learning training and M. Sixt,
S. Boyd and T. Weiss for discussions and critical reading of the manuscript. L.
Lavis (Janelia Research Campus) generously provided the JF585-HaloTag ligand. We
acknowledge expert support by IST\r\nAustria’s scientific computing, imaging and
optics, preclinical, library and laboratory support facilities and by the Miba machine
shop. We gratefully acknowledge funding by the following sources: Austrian Science
Fund (F.W.F.) grant no. I3600-B27 (J.G.D.), grant no. DK W1232\r\n(J.G.D. and J.M.M.)
and grant no. Z 312-B27, Wittgenstein award (P.J.); the Gesellschaft für Forschungsförderung
NÖ grant no. LSC18-022 (J.G.D.); an ISTA Interdisciplinary project grant (J.G.D.
and B.B.); the European Union’s Horizon 2020 research and innovation programme,\r\nMarie-Skłodowska
Curie grant 665385 (J.M.M. and J.L.); the European Union’s Horizon 2020 research
and innovation programme, European Research Council grant no. 715767, MATERIALIZABLE
(B.B.); grant no. 715508, REVERSEAUTISM (G.N.); grant no. 695568, SYNNOVATE (S.G.N.G.);
and grant no. 692692, GIANTSYN (P.J.); the Simons\r\nFoundation Autism Research
Initiative grant no. 529085 (S.G.N.G.); the Wellcome Trust Technology Development
grant no. 202932 (S.G.N.G.); the Marie Skłodowska-Curie Actions Individual Fellowship
no. 101026635 under the EU Horizon 2020 program (J.F.W.);\r\nthe Human Frontier
Science Program postdoctoral fellowship LT000557/2018 (W.J.); and the National Science
Foundation grant no. IIS-1835231 (H.P.) and NCS-FO-2124179 (H.P.)."
article_processing_charge: Yes
article_type: original
author:
- first_name: Philipp
full_name: Velicky, Philipp
id: 39BDC62C-F248-11E8-B48F-1D18A9856A87
last_name: Velicky
orcid: 0000-0002-2340-7431
- first_name: Eder
full_name: Miguel Villalba, Eder
id: 3FB91342-F248-11E8-B48F-1D18A9856A87
last_name: Miguel Villalba
orcid: 0000-0001-5665-0430
- first_name: Julia M
full_name: Michalska, Julia M
id: 443DB6DE-F248-11E8-B48F-1D18A9856A87
last_name: Michalska
orcid: 0000-0003-3862-1235
- first_name: Julia
full_name: Lyudchik, Julia
id: 46E28B80-F248-11E8-B48F-1D18A9856A87
last_name: Lyudchik
- first_name: Donglai
full_name: Wei, Donglai
last_name: Wei
- first_name: Zudi
full_name: Lin, Zudi
last_name: Lin
- first_name: Jake
full_name: Watson, Jake
id: 63836096-4690-11EA-BD4E-32803DDC885E
last_name: Watson
orcid: 0000-0002-8698-3823
- first_name: Jakob
full_name: Troidl, Jakob
last_name: Troidl
- first_name: Johanna
full_name: Beyer, Johanna
last_name: Beyer
- first_name: Yoav
full_name: Ben Simon, Yoav
id: 43DF3136-F248-11E8-B48F-1D18A9856A87
last_name: Ben Simon
- first_name: Christoph M
full_name: Sommer, Christoph M
id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
last_name: Sommer
orcid: 0000-0003-1216-9105
- first_name: Wiebke
full_name: Jahr, Wiebke
id: 425C1CE8-F248-11E8-B48F-1D18A9856A87
last_name: Jahr
- first_name: Alban
full_name: Cenameri, Alban
id: 9ac8f577-2357-11eb-997a-e566c5550886
last_name: Cenameri
- first_name: Johannes
full_name: Broichhagen, Johannes
last_name: Broichhagen
- first_name: Seth G.N.
full_name: Grant, Seth G.N.
last_name: Grant
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: Gaia
full_name: Novarino, Gaia
id: 3E57A680-F248-11E8-B48F-1D18A9856A87
last_name: Novarino
orcid: 0000-0002-7673-7178
- first_name: Hanspeter
full_name: Pfister, Hanspeter
last_name: Pfister
- first_name: Bernd
full_name: Bickel, Bernd
id: 49876194-F248-11E8-B48F-1D18A9856A87
last_name: Bickel
orcid: 0000-0001-6511-9385
- first_name: Johann G
full_name: Danzl, Johann G
id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87
last_name: Danzl
orcid: 0000-0001-8559-3973
citation:
ama: Velicky P, Miguel Villalba E, Michalska JM, et al. Dense 4D nanoscale reconstruction
of living brain tissue. Nature Methods. 2023;20:1256-1265. doi:10.1038/s41592-023-01936-6
apa: Velicky, P., Miguel Villalba, E., Michalska, J. M., Lyudchik, J., Wei, D.,
Lin, Z., … Danzl, J. G. (2023). Dense 4D nanoscale reconstruction of living brain
tissue. Nature Methods. Springer Nature. https://doi.org/10.1038/s41592-023-01936-6
chicago: Velicky, Philipp, Eder Miguel Villalba, Julia M Michalska, Julia Lyudchik,
Donglai Wei, Zudi Lin, Jake Watson, et al. “Dense 4D Nanoscale Reconstruction
of Living Brain Tissue.” Nature Methods. Springer Nature, 2023. https://doi.org/10.1038/s41592-023-01936-6.
ieee: P. Velicky et al., “Dense 4D nanoscale reconstruction of living brain
tissue,” Nature Methods, vol. 20. Springer Nature, pp. 1256–1265, 2023.
ista: Velicky P, Miguel Villalba E, Michalska JM, Lyudchik J, Wei D, Lin Z, Watson
J, Troidl J, Beyer J, Ben Simon Y, Sommer CM, Jahr W, Cenameri A, Broichhagen
J, Grant SGN, Jonas PM, Novarino G, Pfister H, Bickel B, Danzl JG. 2023. Dense
4D nanoscale reconstruction of living brain tissue. Nature Methods. 20, 1256–1265.
mla: Velicky, Philipp, et al. “Dense 4D Nanoscale Reconstruction of Living Brain
Tissue.” Nature Methods, vol. 20, Springer Nature, 2023, pp. 1256–65, doi:10.1038/s41592-023-01936-6.
short: P. Velicky, E. Miguel Villalba, J.M. Michalska, J. Lyudchik, D. Wei, Z. Lin,
J. Watson, J. Troidl, J. Beyer, Y. Ben Simon, C.M. Sommer, W. Jahr, A. Cenameri,
J. Broichhagen, S.G.N. Grant, P.M. Jonas, G. Novarino, H. Pfister, B. Bickel,
J.G. Danzl, Nature Methods 20 (2023) 1256–1265.
date_created: 2023-07-23T22:01:13Z
date_published: 2023-08-01T00:00:00Z
date_updated: 2024-01-10T08:37:48Z
day: '01'
department:
- _id: PeJo
- _id: GaNo
- _id: BeBi
- _id: JoDa
- _id: Bio
doi: 10.1038/s41592-023-01936-6
ec_funded: 1
external_id:
isi:
- '001025621500001'
pmid:
- '37429995'
intvolume: ' 20'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1038/s41592-023-01936-6
month: '08'
oa: 1
oa_version: Published Version
page: 1256-1265
pmid: 1
project:
- _id: 265CB4D0-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03600
name: Optical control of synaptic function via adhesion molecules
- _id: 2548AE96-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: W1232-B24
name: Molecular Drug Targets
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: The Wittgenstein Prize
- _id: 23889792-32DE-11EA-91FC-C7463DDC885E
name: High content imaging to decode human immune cell interactions in health and
allergic disease
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
- _id: 24F9549A-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '715767'
name: 'MATERIALIZABLE: Intelligent fabrication-oriented Computational Design and
Modeling'
- _id: 25444568-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '715508'
name: Probing the Reversibility of Autism Spectrum Disorders by Employing in vivo
and in vitro Models
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: fc2be41b-9c52-11eb-aca3-faa90aa144e9
call_identifier: H2020
grant_number: '101026635'
name: Synaptic computations of the hippocampal CA3 circuitry
- _id: 2668BFA0-B435-11E9-9278-68D0E5697425
grant_number: LT00057
name: High-speed 3D-nanoscopy to study the role of adhesion during 3D cell migration
publication: Nature Methods
publication_identifier:
eissn:
- 1548-7105
issn:
- 1548-7091
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- relation: software
url: https://github.com/danzllab/LIONESS
record:
- id: '12817'
relation: research_data
status: public
- id: '14770'
relation: shorter_version
status: public
scopus_import: '1'
status: public
title: Dense 4D nanoscale reconstruction of living brain tissue
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 20
year: '2023'
...
---
_id: '13126'
abstract:
- lang: eng
text: Mapping the complex and dense arrangement of cells and their connectivity
in brain tissue demands nanoscale spatial resolution imaging. Super-resolution
optical microscopy excels at visualizing specific molecules and individual cells
but fails to provide tissue context. Here, we developed Comprehensive Analysis
of Tissues across Scales (CATS), a technology to densely map brain tissue architecture
from millimeter regional to nanometer synaptic scales in diverse chemically fixed
brain preparations, including rodent and human. CATS uses fixation-compatible
extracellular labeling and optical imaging, including stimulated emission depletion
or expansion microscopy, to comprehensively delineate cellular structures. It
enables three-dimensional reconstruction of single synapses and mapping of synaptic
connectivity by identification and analysis of putative synaptic cleft regions.
Applying CATS to the mouse hippocampal mossy fiber circuitry, we reconstructed
and quantified the synaptic input and output structure of identified neurons.
We furthermore demonstrate applicability to clinically derived human tissue samples,
including formalin-fixed paraffin-embedded routine diagnostic specimens, for visualizing
the cellular architecture of brain tissue in health and disease.
acknowledged_ssus:
- _id: ScienComp
- _id: Bio
- _id: PreCl
- _id: LifeSc
- _id: M-Shop
- _id: E-Lib
acknowledgement: "We thank Jakob Vorlaufer, Nathalie Agudelo-Dueñas, Wiebke Jahr,
Andreas Wartak for microscope maintenance and troubleshooting, Caroline Kreuzinger,
Anna Freeman, and Irene Erber for technical assistance and Matthias Tomschik for
support with obtaining human samples. We gratefully acknowledge Eder Miguel for
setting up webKnossos and Marek Šuplata for computational support and hardware control.
We are grateful to Ryuichi Shigemoto and Bernd Bickel for generous support, and
Michael Sixt and Scott Boyd (Stanford University) for discussions and critical reading
of the manuscript. PSD95-HaloTag mice were kindly provided by Seth Grant (University
of Edinburgh). We acknowledge expert support by IST Austria’s scientific computing,
imaging and optics, preclinical, and lab support facilities, and by the Library
and Miba machine shop.\r\nWe gratefully acknowledge funding by the following sources:
\r\nAustrian Science Fund (FWF) grant I3600-B27 (JGD)\r\nAustrian Science Fund (FWF)
grant DK W1232 (JGD, JMM)\r\nAustrian Science Fund (FWF) grant Z 312-B27, Wittgenstein
award (PJ)\r\nAustrian Science Funds (FWF) projects I4685-B, I6565-B (SYNABS) and
DOC 33-B27 (RH)\r\nGesellschaft für Forschungsförderung NÖ (NFB) grant LSC18-022
(JGD)\r\nEuropean Union’s Horizon 2020 research and innovation programme, European
Research Council (ERC) grant 715508 – REVERSEAUTISM (GN)\r\nEuropean Union’s Horizon
2020 research and innovation programme, European Research Council (ERC) grant 692692
– GIANTSYN (PJ)\r\nMarie Skłodowska-Curie Actions Fellowship GA no. 665385 under
the EU Horizon 2020 program (JMM, JL)\r\nMarie Skłodowska-Curie Actions Individual
Fellowship 101026635 under the EU Horizon 2020 program (JFW)"
article_processing_charge: No
author:
- first_name: Johann G
full_name: Danzl, Johann G
id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87
last_name: Danzl
orcid: 0000-0001-8559-3973
citation:
ama: Danzl JG. Research data for the publication “Imaging brain tissue architecture
across millimeter to nanometer scales.” 2023. doi:10.15479/AT:ISTA:13126
apa: Danzl, J. G. (2023). Research data for the publication “Imaging brain tissue
architecture across millimeter to nanometer scales.” Institute of Science and
Technology Austria. https://doi.org/10.15479/AT:ISTA:13126
chicago: Danzl, Johann G. “Research Data for the Publication ‘Imaging Brain Tissue
Architecture across Millimeter to Nanometer Scales.’” Institute of Science and
Technology Austria, 2023. https://doi.org/10.15479/AT:ISTA:13126.
ieee: J. G. Danzl, “Research data for the publication ‘Imaging brain tissue architecture
across millimeter to nanometer scales.’” Institute of Science and Technology Austria,
2023.
ista: Danzl JG. 2023. Research data for the publication ‘Imaging brain tissue architecture
across millimeter to nanometer scales’, Institute of Science and Technology Austria,
10.15479/AT:ISTA:13126.
mla: Danzl, Johann G. Research Data for the Publication “Imaging Brain Tissue
Architecture across Millimeter to Nanometer Scales.” Institute of Science
and Technology Austria, 2023, doi:10.15479/AT:ISTA:13126.
short: J.G. Danzl, (2023).
contributor:
- first_name: Julia M
id: 443DB6DE-F248-11E8-B48F-1D18A9856A87
last_name: Michalska
- first_name: Julia
id: 46E28B80-F248-11E8-B48F-1D18A9856A87
last_name: Lyudchik
- first_name: Philipp
id: 39BDC62C-F248-11E8-B48F-1D18A9856A87
last_name: Velicky
orcid: 0000-0002-2340-7431
- first_name: Hana
id: ee3cb6ca-ec98-11ea-ae11-ff703e2254ed
last_name: Stefanickova
- first_name: Jake
id: 63836096-4690-11EA-BD4E-32803DDC885E
last_name: Watson
orcid: 0000-0002-8698-3823
- first_name: Alban
id: 9ac8f577-2357-11eb-997a-e566c5550886
last_name: Cenameri
- first_name: Christoph M
id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
last_name: Sommer
orcid: 0000-0003-1216-9105
- first_name: Nicole
id: 4CD6AAC6-F248-11E8-B48F-1D18A9856A87
last_name: Amberg
orcid: 0000-0002-3183-8207
- first_name: Alessandro
id: 41CB84B2-F248-11E8-B48F-1D18A9856A87
last_name: Venturino
orcid: 0000-0003-2356-9403
- first_name: Karl
last_name: Roessler
- first_name: Thomas
last_name: Czech
- first_name: Romana
last_name: Höftberger
- first_name: Sandra
id: 36ACD32E-F248-11E8-B48F-1D18A9856A87
last_name: Siegert
orcid: 0000-0001-8635-0877
- first_name: Gaia
id: 3E57A680-F248-11E8-B48F-1D18A9856A87
last_name: Novarino
orcid: 0000-0002-7673-7178
- first_name: Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
date_created: 2023-06-07T07:15:12Z
date_published: 2023-08-04T00:00:00Z
date_updated: 2024-02-21T12:18:19Z
day: '04'
ddc:
- '610'
department:
- _id: JoDa
- _id: SaSi
- _id: GaNo
- _id: PeJo
- _id: Bio
- _id: RySh
doi: 10.15479/AT:ISTA:13126
ec_funded: 1
file:
- access_level: open_access
checksum: 6f18ce9b89b47ce5abeb379869ff5c49
content_type: text/plain
creator: jdanzl
date_created: 2023-08-04T13:19:47Z
date_updated: 2023-08-04T13:19:47Z
file_id: '13961'
file_name: Readme_Michalska_2023.txt
file_size: 541
relation: main_file
success: 1
- access_level: open_access
checksum: 2098e8c5285c5e86cb69075e1b5dcf39
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:29:29Z
date_updated: 2023-08-03T11:29:29Z
file_id: '13482'
file_name: Fig1_b_top-left.tif
file_size: 64582744
relation: main_file
success: 1
- access_level: open_access
checksum: 9affab3dacf847096cc8e8d5ff99467e
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:29:28Z
date_updated: 2023-08-03T11:29:28Z
file_id: '13483'
file_name: Fig1_b_top-right.tif
file_size: 1442113
relation: main_file
success: 1
- access_level: open_access
checksum: d7fec0296123a17f6bcdbfee17411424
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:29:29Z
date_updated: 2023-08-03T11:29:29Z
file_id: '13484'
file_name: Fig1_c_1.tif
file_size: 40078276
relation: main_file
success: 1
- access_level: open_access
checksum: 5195a180c4f26a7adc994bad0b3c53c7
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:29:29Z
date_updated: 2023-08-03T11:29:29Z
file_id: '13485'
file_name: Fig1_c_2.tif
file_size: 7984315
relation: main_file
success: 1
- access_level: open_access
checksum: df1105d8f41ebd5373cacc597ffa91f3
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:29:29Z
date_updated: 2023-08-03T11:29:29Z
file_id: '13486'
file_name: Fig1_c_3.tif
file_size: 8693320
relation: main_file
success: 1
- access_level: open_access
checksum: 00edbb0c9f42032c830d2291ec55c4c0
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:29:30Z
date_updated: 2023-08-03T11:29:30Z
file_id: '13487'
file_name: Fig1_c_4.tif
file_size: 6003063
relation: main_file
success: 1
- access_level: open_access
checksum: a58399e98ec4b6506a5dae13e387f391
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:29:30Z
date_updated: 2023-08-03T11:29:30Z
file_id: '13488'
file_name: Fig1_d.tif
file_size: 24003063
relation: main_file
success: 1
- access_level: open_access
checksum: 699df4bc104df14607359678963b3d2d
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:29:30Z
date_updated: 2023-08-03T11:29:30Z
file_id: '13489'
file_name: Fig1_e_zoom.tif
file_size: 18063
relation: main_file
success: 1
- access_level: open_access
checksum: ea7b1278da490d221fc7698fd6bc39a4
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:29:30Z
date_updated: 2023-08-03T11:29:30Z
file_id: '13490'
file_name: Fig1_e.tif
file_size: 186813
relation: main_file
success: 1
- access_level: open_access
checksum: 37b05ef3a36b9f6bf4ec46199230874a
content_type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
creator: jdanzl
date_created: 2023-08-03T11:29:30Z
date_updated: 2023-08-03T11:29:30Z
file_id: '13491'
file_name: Fig1_f.xlsx
file_size: 17494
relation: main_file
success: 1
- access_level: open_access
checksum: 872eb3dc1a94afb26947a365bfae581a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:40:43Z
date_updated: 2023-08-03T11:40:43Z
file_id: '13492'
file_name: Fig2_a_1.tif
file_size: 816786862
relation: main_file
success: 1
- access_level: open_access
checksum: cab6f72d7d546a81c105c229747949ef
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:40:13Z
date_updated: 2023-08-03T11:40:13Z
file_id: '13493'
file_name: Fig2_a_2.tif
file_size: 8654692
relation: main_file
success: 1
- access_level: open_access
checksum: c85bc3ffe7f6bdf16a81290761ed6253
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:40:14Z
date_updated: 2023-08-03T11:40:14Z
file_id: '13494'
file_name: Fig2_a_3.tif
file_size: 8654692
relation: main_file
success: 1
- access_level: open_access
checksum: 5a3fd5484e566c34e210a4acb2b97791
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:40:24Z
date_updated: 2023-08-03T11:40:24Z
file_id: '13495'
file_name: Fig2_a_4.tif
file_size: 272262438
relation: main_file
success: 1
- access_level: open_access
checksum: ae5cbacf56e3fdf934a6d6fd489f5fe0
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:40:37Z
date_updated: 2023-08-03T11:40:37Z
file_id: '13496'
file_name: Fig2_a_5.tif
file_size: 272262438
relation: main_file
success: 1
- access_level: open_access
checksum: e6da436b83bbe3a34138b52e5de49d69
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:40:44Z
date_updated: 2023-08-03T11:40:44Z
file_id: '13497'
file_name: Fig2_a_6.tif
file_size: 204301228
relation: main_file
success: 1
- access_level: open_access
checksum: 855f0a0bcf5b3d7cc7d50561f2c8f625
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:40:49Z
date_updated: 2023-08-03T11:40:49Z
file_id: '13498'
file_name: Fig2_a_7-8.tif
file_size: 68094350
relation: main_file
success: 1
- access_level: open_access
checksum: ff709f9f8ebaa8cf50a4b2fca5ce9813
content_type: application/octet-stream
creator: jdanzl
date_created: 2023-08-03T11:40:48Z
date_updated: 2023-08-03T11:40:48Z
file_id: '13499'
file_name: Fig2_a_roi.roi
file_size: 156
relation: main_file
success: 1
- access_level: open_access
checksum: ce9b774ff5163ebfa4e3fb162f4cfffe
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:41:37Z
date_updated: 2023-08-03T11:41:37Z
file_id: '13500'
file_name: Fig2_c-i_1_n2v.tif
file_size: 1036927887
relation: main_file
success: 1
- access_level: open_access
checksum: 21f1bb8d2a00c88bdd6febe6285acc2a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:41:25Z
date_updated: 2023-08-03T11:41:25Z
file_id: '13501'
file_name: Fig2_c-i_1.tif
file_size: 518519275
relation: main_file
success: 1
- access_level: open_access
checksum: 9af5b66b74c2ff4b2b99b0d94f2864e6
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:42:48Z
date_updated: 2023-08-03T11:42:48Z
file_id: '13502'
file_name: Fig2_c-i_3_n2v.tif
file_size: 1444202302
relation: main_file
success: 1
- access_level: open_access
checksum: c83261e88821747b301197314d92f2b2
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:42:57Z
date_updated: 2023-08-03T11:42:57Z
file_id: '13503'
file_name: Fig2_c-i_3.tif
file_size: 1075338708
relation: main_file
success: 1
- access_level: open_access
checksum: 5d1278ba4b7e085c0c186ea49fce535b
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:44:05Z
date_updated: 2023-08-03T11:44:05Z
file_id: '13504'
file_name: Fig2_c-i_2_n2v.tif
file_size: 851145493
relation: main_file
success: 1
- access_level: open_access
checksum: 5aaf1eba65582cf437ff3ce753eba4d7
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:43:47Z
date_updated: 2023-08-03T11:43:47Z
file_id: '13505'
file_name: Fig2_c-i_2.tif
file_size: 432099834
relation: main_file
success: 1
- access_level: open_access
checksum: ce9b774ff5163ebfa4e3fb162f4cfffe
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:44:41Z
date_updated: 2023-08-03T11:44:41Z
file_id: '13506'
file_name: Fig2_h_n2v.tif
file_size: 1036927887
relation: main_file
success: 1
- access_level: open_access
checksum: 21f1bb8d2a00c88bdd6febe6285acc2a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:44:36Z
date_updated: 2023-08-03T11:44:36Z
file_id: '13507'
file_name: Fig2_h.tif
file_size: 518519275
relation: main_file
success: 1
- access_level: open_access
checksum: b37f25a90ef42fa4358649fdd6b41f35
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:44:40Z
date_updated: 2023-08-03T11:44:40Z
file_id: '13508'
file_name: Fig2_j_1.tif
file_size: 19287397
relation: main_file
success: 1
- access_level: open_access
checksum: 50f0969c6c2c1209ff776bec4861030a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:44:41Z
date_updated: 2023-08-03T11:44:41Z
file_id: '13509'
file_name: Fig2_j_2.tif
file_size: 617552
relation: main_file
success: 1
- access_level: open_access
checksum: 2a8cc93471378ac4c3b640e4318d89eb
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:44:41Z
date_updated: 2023-08-03T11:44:41Z
file_id: '13510'
file_name: Fig2_j_3.tif
file_size: 19287392
relation: main_file
success: 1
- access_level: open_access
checksum: b90965d20133fea73c99050c32eb7695
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:44:42Z
date_updated: 2023-08-03T11:44:42Z
file_id: '13511'
file_name: Fig2_j_4.tif
file_size: 617552
relation: main_file
success: 1
- access_level: open_access
checksum: 568a1e0efaa62527b51e66a7aabfd05c
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:44:43Z
date_updated: 2023-08-03T11:44:43Z
file_id: '13512'
file_name: Fig2_j_coCATS_N2V.tif
file_size: 19287339
relation: main_file
success: 1
- access_level: open_access
checksum: c70447cebe073dd78e0ac34ecc36ad85
content_type: application/octet-stream
creator: jdanzl
date_created: 2023-08-03T11:44:44Z
date_updated: 2023-08-03T11:44:44Z
file_id: '13513'
file_name: Fig2_j_roi.roi
file_size: 148
relation: main_file
success: 1
- access_level: open_access
checksum: 505252cc4b443b76c2e41ecf5879a542
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:44:52Z
date_updated: 2023-08-03T11:44:52Z
file_id: '13514'
file_name: Fig3_a_n2v.tif
file_size: 316842690
relation: main_file
success: 1
- access_level: open_access
checksum: 35463183611e8ee4fba2025772c40eb2
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:45:01Z
date_updated: 2023-08-03T11:45:01Z
file_id: '13515'
file_name: Fig3_a.tif
file_size: 172839057
relation: main_file
success: 1
- access_level: open_access
checksum: e0469102a87332b46904b4ba35b8fb2f
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:45:05Z
date_updated: 2023-08-03T11:45:05Z
file_id: '13516'
file_name: Fig3_g_n2v.tif
file_size: 309407894
relation: main_file
success: 1
- access_level: open_access
checksum: 854c8fe02acd920f8e8f6a3173458e86
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:51:15Z
date_updated: 2023-08-03T11:51:15Z
file_id: '13517'
file_name: Fig4_a_MIP.tif
file_size: 11327792
relation: main_file
success: 1
- access_level: open_access
checksum: ab35811ffbe3f40733d0e054d9d6c4e6
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:51:18Z
date_updated: 2023-08-03T11:51:18Z
file_id: '13518'
file_name: Fig4_c_dghilus_bouton2_n2v.tif
file_size: 106696700
relation: main_file
success: 1
- access_level: open_access
checksum: e3b6410d68e42004ef3857967707aeb3
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:51:17Z
date_updated: 2023-08-03T11:51:17Z
file_id: '13519'
file_name: Fig4_c_dghilus_bouton2.tif
file_size: 53367287
relation: main_file
success: 1
- access_level: open_access
checksum: 9ef0e3f3ec6f0b7b2666e3a0d7e6f355
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:51:20Z
date_updated: 2023-08-03T11:51:20Z
file_id: '13520'
file_name: Fig4_c_dghilus_bouton5_n2v.tif
file_size: 103816699
relation: main_file
success: 1
- access_level: open_access
checksum: d5d846a4edaf0168ca6cc53d8cc39dde
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:51:21Z
date_updated: 2023-08-03T11:51:21Z
file_id: '13521'
file_name: Fig4_c_dghilus_bouton5.tif
file_size: 51927287
relation: main_file
success: 1
- access_level: open_access
checksum: 690a87182441c9742da2550189641e5a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:51:24Z
date_updated: 2023-08-03T11:51:24Z
file_id: '13522'
file_name: Fig4_c_stratum_bouton11_n2v.tif
file_size: 76391971
relation: main_file
success: 1
- access_level: open_access
checksum: 4dc575424729c5ba181bd9a3962d4781
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:51:23Z
date_updated: 2023-08-03T11:51:23Z
file_id: '13523'
file_name: Fig4_c_stratum_bouton11.tif
file_size: 38217920
relation: main_file
success: 1
- access_level: open_access
checksum: 9fdd51636928f45e8e534cc5a37a48ff
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:51:29Z
date_updated: 2023-08-03T11:51:29Z
file_id: '13524'
file_name: Fig4_c_stratum_bouton13_n2v.tif
file_size: 154149020
relation: main_file
success: 1
- access_level: open_access
checksum: 643169464252d67753ac6f8493668f34
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:51:28Z
date_updated: 2023-08-03T11:51:28Z
file_id: '13525'
file_name: Fig4_c_stratum_bouton13.tif
file_size: 77099447
relation: main_file
success: 1
- access_level: open_access
checksum: ab35811ffbe3f40733d0e054d9d6c4e6
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:51:32Z
date_updated: 2023-08-03T11:51:32Z
file_id: '13526'
file_name: Fig4_d.tif
file_size: 106696700
relation: main_file
success: 1
- access_level: open_access
checksum: 9fdd51636928f45e8e534cc5a37a48ff
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:51:35Z
date_updated: 2023-08-03T11:51:35Z
file_id: '13527'
file_name: Fig4_e.tif
file_size: 154149020
relation: main_file
success: 1
- access_level: open_access
checksum: 5380d0a98e97d7d8462b38bd0a96644c
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:51:32Z
date_updated: 2023-08-03T11:51:32Z
file_id: '13528'
file_name: Fig4_f_1_top.tif
file_size: 5986760
relation: main_file
success: 1
- access_level: open_access
checksum: 7d1309e806eb31588ebafcf40388e13f
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:51:33Z
date_updated: 2023-08-03T11:51:33Z
file_id: '13529'
file_name: Fig4_f_2_top.tif
file_size: 6001178
relation: main_file
success: 1
- access_level: open_access
checksum: a71c80207655d508cbc7626bc144d7a1
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:51:33Z
date_updated: 2023-08-03T11:51:33Z
file_id: '13530'
file_name: Fig4_f_3_top.tif
file_size: 6023318
relation: main_file
success: 1
- access_level: open_access
checksum: 5d76b2ad7d7acbb1afa27cf52194b1cb
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:51:34Z
date_updated: 2023-08-03T11:51:34Z
file_id: '13531'
file_name: Fig4_f_4_top.tif
file_size: 6038186
relation: main_file
success: 1
- access_level: open_access
checksum: 9cee6b3387bd880843f2c6cdfa0d4a8d
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:51:34Z
date_updated: 2023-08-03T11:51:34Z
file_id: '13532'
file_name: Fig4_f_5_top.tif
file_size: 2324158
relation: main_file
success: 1
- access_level: open_access
checksum: a48fc9e1bb2710cf28466ae6d2a20c19
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T11:51:34Z
date_updated: 2023-08-03T11:51:34Z
file_id: '13533'
file_name: Fig4_f_1_bottom.png
file_size: 1150264
relation: main_file
success: 1
- access_level: open_access
checksum: ff47ca30f814e17561974b73b0913a1a
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T11:51:34Z
date_updated: 2023-08-03T11:51:34Z
file_id: '13534'
file_name: Fig4_f_2_bottom.png
file_size: 1157424
relation: main_file
success: 1
- access_level: open_access
checksum: 2ea3fb8d79cee8ae9de7ca31d003910f
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T11:51:34Z
date_updated: 2023-08-03T11:51:34Z
file_id: '13535'
file_name: Fig4_f_3_bottom.png
file_size: 1152715
relation: main_file
success: 1
- access_level: open_access
checksum: d25e5ecf2f0c3be7c20a7e25a7a334b8
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T11:51:34Z
date_updated: 2023-08-03T11:51:34Z
file_id: '13536'
file_name: Fig4_f_4_bottom.png
file_size: 1105240
relation: main_file
success: 1
- access_level: open_access
checksum: 76535f569a57b967e378df89b7d2982a
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T11:51:35Z
date_updated: 2023-08-03T11:51:35Z
file_id: '13537'
file_name: Fig4_f_5_bottom.png
file_size: 1228157
relation: main_file
success: 1
- access_level: open_access
checksum: d46fefe73daa3ec08b847631e9de6828
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:53:52Z
date_updated: 2023-08-03T11:53:52Z
file_id: '13538'
file_name: Fig5_a_1.tif
file_size: 91181184
relation: main_file
success: 1
- access_level: open_access
checksum: 354c0ba876a33672eca37cd61dac7073
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:53:54Z
date_updated: 2023-08-03T11:53:54Z
file_id: '13539'
file_name: Fig5_a_2.tif
file_size: 176024124
relation: main_file
success: 1
- access_level: open_access
checksum: d07925040ef0c3c51edbdca8cf3ec1fa
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:53:53Z
date_updated: 2023-08-03T11:53:53Z
file_id: '13540'
file_name: Fig5_a_3.tif
file_size: 15812494
relation: main_file
success: 1
- access_level: open_access
checksum: 7c34b19a1764e2f501f11dcabd442ce2
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:53:53Z
date_updated: 2023-08-03T11:53:53Z
file_id: '13541'
file_name: Fig5_a_4.tif
file_size: 11117689
relation: main_file
success: 1
- access_level: open_access
checksum: c49af4b96532e00b3a51574a2a94decb
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:53:53Z
date_updated: 2023-08-03T11:53:53Z
file_id: '13542'
file_name: Fig5_a_5.tif
file_size: 111665
relation: main_file
success: 1
- access_level: open_access
checksum: 1b42eadcdd71f2dc00d82784673aa577
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:53:54Z
date_updated: 2023-08-03T11:53:54Z
file_id: '13543'
file_name: Fig5_b_1.tif
file_size: 16676449
relation: main_file
success: 1
- access_level: open_access
checksum: 0d27266bf216f71fcb5496731ce76dd5
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:53:54Z
date_updated: 2023-08-03T11:53:54Z
file_id: '13544'
file_name: Fig5_b_2-4.tif
file_size: 427651
relation: main_file
success: 1
- access_level: open_access
checksum: c4232930068a0a8f7c8a70ce0ea48dc2
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:53:55Z
date_updated: 2023-08-03T11:53:55Z
file_id: '13545'
file_name: Fig5_c_1-3.tif
file_size: 11117705
relation: main_file
success: 1
- access_level: open_access
checksum: c9caad749be0ef04ef8013cfdb69f8fc
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:53:55Z
date_updated: 2023-08-03T11:53:55Z
file_id: '13546'
file_name: Fig5_c_2-4.tif
file_size: 254153
relation: main_file
success: 1
- access_level: open_access
checksum: 5c6d36b96bde1e090cc428b269aba6ec
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:54:14Z
date_updated: 2023-08-03T11:54:14Z
file_id: '13547'
file_name: Fig5_e_1.tif
file_size: 737290746
relation: main_file
success: 1
- access_level: open_access
checksum: f97632984b6a73e06cc0c8c947cdcc71
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:53:57Z
date_updated: 2023-08-03T11:53:57Z
file_id: '13548'
file_name: Fig5_e_2.tif
file_size: 31366288
relation: main_file
success: 1
- access_level: open_access
checksum: 8c471eeaa59008eed9e91afe5e1f1698
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:53:57Z
date_updated: 2023-08-03T11:53:57Z
file_id: '13549'
file_name: Fig5_e_3.tif
file_size: 1216304
relation: main_file
success: 1
- access_level: open_access
checksum: 31c10c3a1d874b5028e35e2dd8800a68
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:53:59Z
date_updated: 2023-08-03T11:53:59Z
file_id: '13550'
file_name: Fig5_g.tif
file_size: 35669193
relation: main_file
success: 1
- access_level: open_access
checksum: 0e9fe7b7ee06a630837e9d3c41b80e01
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:53:59Z
date_updated: 2023-08-03T11:53:59Z
file_id: '13551'
file_name: Fig5_h.tif
file_size: 7492349
relation: main_file
success: 1
- access_level: open_access
checksum: d25b3d04bb8364089cafdf8deb6846b6
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:59:18Z
date_updated: 2023-08-03T11:59:18Z
file_id: '13552'
file_name: Fig6_a_1.tif
file_size: 121446390
relation: main_file
success: 1
- access_level: open_access
checksum: 4361e10db6ec48c2d4579ac089ff3ca3
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:59:22Z
date_updated: 2023-08-03T11:59:22Z
file_id: '13553'
file_name: Fig6_a_2.tif
file_size: 264412158
relation: main_file
success: 1
- access_level: open_access
checksum: fe3a00ae75fb3388438e53ced92a9498
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:59:21Z
date_updated: 2023-08-03T11:59:21Z
file_id: '13554'
file_name: Fig6_a_3.tif
file_size: 57399051
relation: main_file
success: 1
- access_level: open_access
checksum: 4372c13d6dd2274aa749799b8e96bf06
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:59:21Z
date_updated: 2023-08-03T11:59:21Z
file_id: '13555'
file_name: Fig6_b_left.tif
file_size: 6003163
relation: main_file
success: 1
- access_level: open_access
checksum: f7ae4e50f2f4d414c598bddd691b722a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:59:21Z
date_updated: 2023-08-03T11:59:21Z
file_id: '13556'
file_name: Fig6_b_right.tif
file_size: 511265
relation: main_file
success: 1
- access_level: open_access
checksum: cde658a9ca666bdef1b29613ab72092d
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:59:39Z
date_updated: 2023-08-03T11:59:39Z
file_id: '13557'
file_name: Fig6_c_n2v.tif
file_size: 467125933
relation: main_file
success: 1
- access_level: open_access
checksum: 57e73c1fedcee4bd8ab62518baa0e0e5
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:59:36Z
date_updated: 2023-08-03T11:59:36Z
file_id: '13558'
file_name: Fig6_c.tif
file_size: 322251440
relation: main_file
success: 1
- access_level: open_access
checksum: 53175073b83ab569241ae64ef7f180b3
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:59:38Z
date_updated: 2023-08-03T11:59:38Z
file_id: '13559'
file_name: Fig6_d_1.tif
file_size: 37641738
relation: main_file
success: 1
- access_level: open_access
checksum: af97423e1077af85465a5e39fa25d1e4
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:59:40Z
date_updated: 2023-08-03T11:59:40Z
file_id: '13560'
file_name: Fig6_d_2.tif
file_size: 70596929
relation: main_file
success: 1
- access_level: open_access
checksum: f1fd7a9fa2a12a930fe35bdf53ba3ff7
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:59:42Z
date_updated: 2023-08-03T11:59:42Z
file_id: '13561'
file_name: Fig6_d_3.tif
file_size: 15303146
relation: main_file
success: 1
- access_level: open_access
checksum: 95655df5cdb05f09912298de9a3711b4
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:59:41Z
date_updated: 2023-08-03T11:59:41Z
file_id: '13562'
file_name: Fig6_d_4.tif
file_size: 893051
relation: main_file
success: 1
- access_level: open_access
checksum: 4d722b4e09ce617252d284f78ea2ee30
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:59:58Z
date_updated: 2023-08-03T11:59:58Z
file_id: '13563'
file_name: Fig6_e.tif
file_size: 381232200
relation: main_file
success: 1
- access_level: open_access
checksum: d1eda9599f422a614404dd112f0c3185
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T11:59:58Z
date_updated: 2023-08-03T11:59:58Z
file_id: '13564'
file_name: Fig6_f.tif
file_size: 345804470
relation: main_file
success: 1
- access_level: open_access
checksum: 1cd202570f964b78e1f55c987d5d5de1
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:00:08Z
date_updated: 2023-08-03T12:00:08Z
file_id: '13565'
file_name: Fig6_g.tif
file_size: 346119927
relation: main_file
success: 1
- access_level: open_access
checksum: 78cbf1906ff8746ed524c62f8f580f52
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:00:02Z
date_updated: 2023-08-03T12:00:02Z
file_id: '13566'
file_name: Fig6_h.tif
file_size: 7064977
relation: main_file
success: 1
- access_level: open_access
checksum: 21635ce59f1ae5a334aaad3f4c0b171c
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:00:03Z
date_updated: 2023-08-03T12:00:03Z
file_id: '13567'
file_name: Fig6_i.tif
file_size: 7110129
relation: main_file
success: 1
- access_level: open_access
checksum: 704962856f2a024ce1f321a1785556e3
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:00:07Z
date_updated: 2023-08-03T12:00:07Z
file_id: '13568'
file_name: Fig6_j.tif
file_size: 110297540
relation: main_file
success: 1
- access_level: open_access
checksum: 4b74ba5ae1e2bb2201bf51c74d733268
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:00:07Z
date_updated: 2023-08-03T12:00:07Z
file_id: '13569'
file_name: Fig6_k.tif
file_size: 11117689
relation: main_file
success: 1
- access_level: open_access
checksum: a8efa3007df4e9e0f80c7a2bbc451178
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:05:41Z
date_updated: 2023-08-03T12:05:41Z
file_id: '13570'
file_name: Extdata_01_a_1.tif
file_size: 1287107
relation: main_file
success: 1
- access_level: open_access
checksum: 7f24b6c84ce96a67347eb5ef4c43fac1
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:05:41Z
date_updated: 2023-08-03T12:05:41Z
file_id: '13571'
file_name: Extdata_01_a_2.tif
file_size: 58307
relation: main_file
success: 1
- access_level: open_access
checksum: fa7ae4eefc11b9b60c4ab80f6e839b73
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:05:41Z
date_updated: 2023-08-03T12:05:41Z
file_id: '13572'
file_name: Extdata_01_a_3.tif
file_size: 1283209
relation: main_file
success: 1
- access_level: open_access
checksum: cec9a5eb87f1b56e64626a00c6d0e53d
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:05:41Z
date_updated: 2023-08-03T12:05:41Z
file_id: '13573'
file_name: Extdata_01_a_4.tif
file_size: 54409
relation: main_file
success: 1
- access_level: open_access
checksum: 450e4786d66d5059028420153908a4dc
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T12:05:41Z
date_updated: 2023-08-03T12:05:41Z
file_id: '13574'
file_name: Extdata_01_c_1.png
file_size: 2431
relation: main_file
success: 1
- access_level: open_access
checksum: feb758609b399cf644dd3ae7cd75fb96
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T12:05:41Z
date_updated: 2023-08-03T12:05:41Z
file_id: '13575'
file_name: Extdata_01_c_2.png
file_size: 612
relation: main_file
success: 1
- access_level: open_access
checksum: 52f43300a50f83f9a6ff1409324086ee
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:05:41Z
date_updated: 2023-08-03T12:05:41Z
file_id: '13576'
file_name: Extdata_01_d_1.tif
file_size: 433981
relation: main_file
success: 1
- access_level: open_access
checksum: 28925dcf8792f524e286beb16b0c532c
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:05:41Z
date_updated: 2023-08-03T12:05:41Z
file_id: '13577'
file_name: Extdata_01_d_2.tif
file_size: 19649
relation: main_file
success: 1
- access_level: open_access
checksum: c93b2f0426c80d18e252435f1e146568
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:05:41Z
date_updated: 2023-08-03T12:05:41Z
file_id: '13578'
file_name: Extdata_01_d_3.tif
file_size: 433981
relation: main_file
success: 1
- access_level: open_access
checksum: c0ed722a768001431f47646ef98c44da
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:05:41Z
date_updated: 2023-08-03T12:05:41Z
file_id: '13579'
file_name: Extdata_01_d_4.tif
file_size: 19649
relation: main_file
success: 1
- access_level: open_access
checksum: db9d386276b985b8e702f797e910bd0b
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T12:05:41Z
date_updated: 2023-08-03T12:05:41Z
file_id: '13580'
file_name: Extdata_01_f_1.png
file_size: 4752
relation: main_file
success: 1
- access_level: open_access
checksum: 244f6645d191c50d997c0dc6a15535ec
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T12:05:41Z
date_updated: 2023-08-03T12:05:41Z
file_id: '13581'
file_name: Extdata_01_f_2.png
file_size: 2698
relation: main_file
success: 1
- access_level: open_access
checksum: 3d6100b836d850b38e66e0f5a7919495
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:07:27Z
date_updated: 2023-08-03T12:07:27Z
file_id: '13582'
file_name: Extdata_02_a.tif
file_size: 24003247
relation: main_file
success: 1
- access_level: open_access
checksum: 3cd6a3b7bf2061cf5c7810c0001236e5
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:07:27Z
date_updated: 2023-08-03T12:07:27Z
file_id: '13583'
file_name: Extdata_02_b.tif
file_size: 1164723
relation: main_file
success: 1
- access_level: open_access
checksum: d87c82d663ffea379cc86c56981e2e16
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:10:32Z
date_updated: 2023-08-03T12:10:32Z
file_id: '13584'
file_name: Extdata_03_SYPH1_xy_1.tif
file_size: 7109763
relation: main_file
success: 1
- access_level: open_access
checksum: a3703d0d26393578d4ba466681fc6be8
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:10:32Z
date_updated: 2023-08-03T12:10:32Z
file_id: '13585'
file_name: Extdata_03_SYPH1_xz_1-2.tif
file_size: 27791
relation: main_file
success: 1
- access_level: open_access
checksum: f653ac7d531cf9896cbd6a9edf2a7759
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:10:32Z
date_updated: 2023-08-03T12:10:32Z
file_id: '13586'
file_name: Extdata_03_SYPH1_xz_3-4.tif
file_size: 27721
relation: main_file
success: 1
- access_level: open_access
checksum: 04edbb337df4d5470e033a4f53c07300
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:10:33Z
date_updated: 2023-08-03T12:10:33Z
file_id: '13587'
file_name: Extdata_03_SYPH1_xz_n2v.tif
file_size: 57657482
relation: main_file
success: 1
- access_level: open_access
checksum: 09e1fe2dd3a1e8349693fda55d0985ca
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:10:34Z
date_updated: 2023-08-03T12:10:34Z
file_id: '13588'
file_name: Extdata_03_SYPH1_xz.tif
file_size: 57657527
relation: main_file
success: 1
- access_level: open_access
checksum: 458f8d486cb63876c6664cde4a9be27c
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:12:25Z
date_updated: 2023-08-03T12:12:25Z
file_id: '13589'
file_name: Extdata_03_SYP1_xy_1.tif
file_size: 7109763
relation: main_file
success: 1
- access_level: open_access
checksum: e2dfccc882d85b660d65a596e1a8cbac
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:12:25Z
date_updated: 2023-08-03T12:12:25Z
file_id: '13590'
file_name: Extdata_03_SYP1_xy_2-3.tif
file_size: 72963
relation: main_file
success: 1
- access_level: open_access
checksum: a331be6052c68cc027cdb79f15447d9a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:12:25Z
date_updated: 2023-08-03T12:12:25Z
file_id: '13591'
file_name: Extdata_03_SYP1_xz_1-2.tif
file_size: 27724
relation: main_file
success: 1
- access_level: open_access
checksum: 4de626b035b43feaacd923fca411e6a2
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:12:25Z
date_updated: 2023-08-03T12:12:25Z
file_id: '13592'
file_name: Extdata_03_SYP1_xz_3-4.tif
file_size: 27724
relation: main_file
success: 1
- access_level: open_access
checksum: d8076e927a074f024924d9bdb6fdd5e6
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:12:30Z
date_updated: 2023-08-03T12:12:30Z
file_id: '13593'
file_name: Extdata_03_SYP1_xz_n2v.tif
file_size: 214613644
relation: main_file
success: 1
- access_level: open_access
checksum: 79cf7d0f450c397caa877dd06b52f8df
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:12:30Z
date_updated: 2023-08-03T12:12:30Z
file_id: '13594'
file_name: Extdata_03_SYP1_xz.tif
file_size: 214613687
relation: main_file
success: 1
- access_level: open_access
checksum: 60ac6ea988d9b8bdaba2f09e0e1ef370
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:14:29Z
date_updated: 2023-08-03T12:14:29Z
file_id: '13595'
file_name: Extdata03_VAMP2_xy_1.tif
file_size: 7109747
relation: main_file
success: 1
- access_level: open_access
checksum: 92bf312da2dd356aa4fc9a0e7d811af2
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:14:29Z
date_updated: 2023-08-03T12:14:29Z
file_id: '13596'
file_name: Extdata03_VAMP2_xy_2-3.tif
file_size: 72947
relation: main_file
success: 1
- access_level: open_access
checksum: 9319e293092eeee24e3c658fc178af1a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:14:29Z
date_updated: 2023-08-03T12:14:29Z
file_id: '13597'
file_name: Extdata03_VAMP2_xz_1-2.tif
file_size: 27724
relation: main_file
success: 1
- access_level: open_access
checksum: a627d7fd9a89bdde968e6d9bff846254
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:14:29Z
date_updated: 2023-08-03T12:14:29Z
file_id: '13598'
file_name: Extdata03_VAMP2_xz_3-4.tif
file_size: 27708
relation: main_file
success: 1
- access_level: open_access
checksum: 7a5363a185edce59ce57535f7d855092
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:14:32Z
date_updated: 2023-08-03T12:14:32Z
file_id: '13599'
file_name: Extdata03_VAMP2_xz_n2v.tif
file_size: 102457485
relation: main_file
success: 1
- access_level: open_access
checksum: 8e7fc4d00fff8ec6d9340e969f37e176
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:14:32Z
date_updated: 2023-08-03T12:14:32Z
file_id: '13600'
file_name: Extdata03_VAMP2_xz.tif
file_size: 102457527
relation: main_file
success: 1
- access_level: open_access
checksum: be17472f73ed8cd02a700b861f1dbac0
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:16:08Z
date_updated: 2023-08-03T12:16:08Z
file_id: '13601'
file_name: Extdata04_VGLUT_xy_1.tif
file_size: 11117763
relation: main_file
success: 1
- access_level: open_access
checksum: dbc3ec477f491ce3e98ab40475bd90ca
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:16:08Z
date_updated: 2023-08-03T12:16:08Z
file_id: '13602'
file_name: Extdata04_VGLUT_xy_2-3.tif
file_size: 72947
relation: main_file
success: 1
- access_level: open_access
checksum: 8ee877e6b843bb0e38febb0481962d69
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:16:08Z
date_updated: 2023-08-03T12:16:08Z
file_id: '13603'
file_name: Extdata04_VGLUT_xz_1-2.tif
file_size: 53367
relation: main_file
success: 1
- access_level: open_access
checksum: 9873f54d50955a2c204d0559c87aa13d
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:16:08Z
date_updated: 2023-08-03T12:16:08Z
file_id: '13604'
file_name: Extdata04_VGLUT_xz_3-4.tif
file_size: 53358
relation: main_file
success: 1
- access_level: open_access
checksum: 8eda08ae5981fcfa3758d0320b1923af
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:16:11Z
date_updated: 2023-08-03T12:16:11Z
file_id: '13605'
file_name: Extdata04_VGLUT_xz_n2v.tif
file_size: 144076208
relation: main_file
success: 1
- access_level: open_access
checksum: bc0b10229d498f60996a5b05cb87c428
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:16:10Z
date_updated: 2023-08-03T12:16:10Z
file_id: '13606'
file_name: Extdata04_VGLUT_xz.tif
file_size: 72071440
relation: main_file
success: 1
- access_level: open_access
checksum: e8d093ce7d98ea5647d8a80e3084c40b
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:32:32Z
date_updated: 2023-08-03T12:32:32Z
file_id: '13607'
file_name: Extdata03_VGAT_xy_1.tif
file_size: 7109763
relation: main_file
success: 1
- access_level: open_access
checksum: 36590ca7a586de7ad76d3ad659c38995
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:32:32Z
date_updated: 2023-08-03T12:32:32Z
file_id: '13608'
file_name: Extdata03_VGAT_xy_2-3.tif
file_size: 72947
relation: main_file
success: 1
- access_level: open_access
checksum: 3914c7709f69d4d18f23a9fb6dc64572
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:32:32Z
date_updated: 2023-08-03T12:32:32Z
file_id: '13609'
file_name: Extdata03_VGAT_xz_1-2.tif
file_size: 53355
relation: main_file
success: 1
- access_level: open_access
checksum: fa98aa521adaefb9e4476fe519e88e59
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:32:32Z
date_updated: 2023-08-03T12:32:32Z
file_id: '13610'
file_name: Extdata03_VGAT_xz_3-4.tif
file_size: 53339
relation: main_file
success: 1
- access_level: open_access
checksum: cd6f384c51756977e37f45043c913252
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:32:36Z
date_updated: 2023-08-03T12:32:36Z
file_id: '13611'
file_name: Extdata03_VGAT_xz_n2v.tif
file_size: 204861338
relation: main_file
success: 1
- access_level: open_access
checksum: fb7e46feda5ac07877a91ad963da0e31
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:32:35Z
date_updated: 2023-08-03T12:32:35Z
file_id: '13612'
file_name: Extdata03_VGAT_xz.tif
file_size: 102457527
relation: main_file
success: 1
- access_level: open_access
checksum: fe033e0a377d391cd8e4af04987d9900
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T12:36:46Z
date_updated: 2023-08-03T12:36:46Z
file_id: '13613'
file_name: Extdata04_MUNC_xy_1.png
file_size: 4348932
relation: main_file
success: 1
- access_level: open_access
checksum: 771d8e2b9192b6620f54ab30e4fad594
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:35:58Z
date_updated: 2023-08-03T12:35:58Z
file_id: '13614'
file_name: Extdata04_MUNC_xy_2-3.tif
file_size: 72947
relation: main_file
success: 1
- access_level: open_access
checksum: 4256d8f06fa2077b352776cf76b46618
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:35:58Z
date_updated: 2023-08-03T12:35:58Z
file_id: '13615'
file_name: Extdata04_MUNC_xz_1-2.tif
file_size: 53435
relation: main_file
success: 1
- access_level: open_access
checksum: 0b9365638c81d545e773170cf04b04b8
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:35:58Z
date_updated: 2023-08-03T12:35:58Z
file_id: '13616'
file_name: Extdata04_MUNC_xz_3-4.tif
file_size: 55941
relation: main_file
success: 1
- access_level: open_access
checksum: 9fa62688dab03c592cec47bf5d1c304d
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:36:04Z
date_updated: 2023-08-03T12:36:04Z
file_id: '13617'
file_name: Extdata04_MUNC_xz_n2v.tif
file_size: 204861404
relation: main_file
success: 1
- access_level: open_access
checksum: 39f710080a26136dbbe4c8916b434c65
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:36:02Z
date_updated: 2023-08-03T12:36:02Z
file_id: '13618'
file_name: Extdata04_MUNC_xz.tif
file_size: 102457527
relation: main_file
success: 1
- access_level: open_access
checksum: eaabc8112645a73f4326e621b4e6ad0a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:38:39Z
date_updated: 2023-08-03T12:38:39Z
file_id: '13619'
file_name: Extdata04_BASSOON_xy_1.tif
file_size: 11117757
relation: main_file
success: 1
- access_level: open_access
checksum: 15f624d29c473ce6e0ae7e466efc059a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:38:39Z
date_updated: 2023-08-03T12:38:39Z
file_id: '13620'
file_name: Extdata04_BASSOON_xy_2-3.tif
file_size: 74041
relation: main_file
success: 1
- access_level: open_access
checksum: 1cb4b4d219767a30072c804a07140240
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:38:39Z
date_updated: 2023-08-03T12:38:39Z
file_id: '13621'
file_name: Extdata04_BASSOON_xz_1-2.tif
file_size: 53435
relation: main_file
success: 1
- access_level: open_access
checksum: 1bac9c192da9375d81cb3747f48c9be4
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:38:39Z
date_updated: 2023-08-03T12:38:39Z
file_id: '13622'
file_name: Extdata04_BASSOON_xz_3-4.tif
file_size: 53362
relation: main_file
success: 1
- access_level: open_access
checksum: 57fa91bfb1e2937e3449e57096b6fc74
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:38:50Z
date_updated: 2023-08-03T12:38:50Z
file_id: '13623'
file_name: Extdata04_BASSOON_xz_n2v.tif
file_size: 576071410
relation: main_file
success: 1
- access_level: open_access
checksum: ac51a734dcf7d1b94afcd4a471f10626
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:38:46Z
date_updated: 2023-08-03T12:38:46Z
file_id: '13624'
file_name: Extdata04_BASSOON_xz.tif
file_size: 288066640
relation: main_file
success: 1
- access_level: open_access
checksum: 244043f7acba250d560e29123702dcd7
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:40:57Z
date_updated: 2023-08-03T12:40:57Z
file_id: '13625'
file_name: Extdata04_HOMER1_xy_1.tif
file_size: 7109763
relation: main_file
success: 1
- access_level: open_access
checksum: eff51ff97539f6491989bfc3d3f88860
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:40:57Z
date_updated: 2023-08-03T12:40:57Z
file_id: '13626'
file_name: Extdata04_HOMER1_xy_2-3.tif
file_size: 72963
relation: main_file
success: 1
- access_level: open_access
checksum: df35046d9bca4e97a5625c5a55218cfb
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:40:57Z
date_updated: 2023-08-03T12:40:57Z
file_id: '13627'
file_name: Extdata04_HOMER1_xz_1-2.tif
file_size: 53375
relation: main_file
success: 1
- access_level: open_access
checksum: 5dc7536f7aaea084dfc329537d8412ce
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:40:57Z
date_updated: 2023-08-03T12:40:57Z
file_id: '13628'
file_name: Extdata04_HOMER1_xz_3-4.tif
file_size: 53359
relation: main_file
success: 1
- access_level: open_access
checksum: 0b407669a26b9d7730a41361ceffda9a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:41:00Z
date_updated: 2023-08-03T12:41:00Z
file_id: '13629'
file_name: Extdata04_HOMER1_xz_n2v.tif
file_size: 204861339
relation: main_file
success: 1
- access_level: open_access
checksum: f95a0da9bea8b605fc2ed310190836b3
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:40:59Z
date_updated: 2023-08-03T12:40:59Z
file_id: '13630'
file_name: Extdata04_HOMER1_xz.tif
file_size: 102457527
relation: main_file
success: 1
- access_level: open_access
checksum: cf74307696548e7aa90e59a0935a74a1
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:45:08Z
date_updated: 2023-08-03T12:45:08Z
file_id: '13631'
file_name: Extdata04_SHANK2_xy_1.tif
file_size: 7120361
relation: main_file
success: 1
- access_level: open_access
checksum: 7b3490246df4f5bb68d0df682161820f
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:45:08Z
date_updated: 2023-08-03T12:45:08Z
file_id: '13632'
file_name: Extdata04_SHANK2_xy_2-3.tif
file_size: 72973
relation: main_file
success: 1
- access_level: open_access
checksum: c599d6830e61c386219e266f4717fe7c
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:45:08Z
date_updated: 2023-08-03T12:45:08Z
file_id: '13633'
file_name: Extdata04_SHANK2_xz_1-2.tif
file_size: 53443
relation: main_file
success: 1
- access_level: open_access
checksum: 5b9912b2a408d224ad669f39dc8d5bc6
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:45:08Z
date_updated: 2023-08-03T12:45:08Z
file_id: '13634'
file_name: Extdata04_SHANK2_xz_3-4.tif
file_size: 53362
relation: main_file
success: 1
- access_level: open_access
checksum: 17f2064ceeb99cbb51208c27b1961ed5
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:45:30Z
date_updated: 2023-08-03T12:45:30Z
file_id: '13635'
file_name: Extdata04_SHANK2_xz_n2v.tif
file_size: 691291157
relation: main_file
success: 1
- access_level: open_access
checksum: 21f1bb8d2a00c88bdd6febe6285acc2a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:45:26Z
date_updated: 2023-08-03T12:45:26Z
file_id: '13636'
file_name: Extdata04_SHANK2_xz.tif
file_size: 518519275
relation: main_file
success: 1
- access_level: open_access
checksum: 47597ca873f1ea81cd4f7e9bfbb34aa4
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:46:25Z
date_updated: 2023-08-03T12:46:25Z
file_id: '13637'
file_name: Extdata04_GEPHYRIN_xy_1.tif
file_size: 752109
relation: main_file
success: 1
- access_level: open_access
checksum: 14ed7ae64eb783e6b30f824f21959d0f
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:46:25Z
date_updated: 2023-08-03T12:46:25Z
file_id: '13638'
file_name: Extdata04_GEPHYRIN_xy_2-3.tif
file_size: 72909
relation: main_file
success: 1
- access_level: open_access
checksum: e361e226428d7b23618eed35b1cad080
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:46:25Z
date_updated: 2023-08-03T12:46:25Z
file_id: '13639'
file_name: Extdata04_GEPHYRIN_xz_1-2.tif
file_size: 53375
relation: main_file
success: 1
- access_level: open_access
checksum: abe7cee22f69fc2bf1a3ff9955ccf45a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:46:25Z
date_updated: 2023-08-03T12:46:25Z
file_id: '13640'
file_name: Extdata04_GEPHYRIN_xz_3-4.tif
file_size: 53360
relation: main_file
success: 1
- access_level: open_access
checksum: 59b49b451a63c5ac06fa31a17640c1a2
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:46:27Z
date_updated: 2023-08-03T12:46:27Z
file_id: '13641'
file_name: Extdata04_GEPHYRIN_xz_n2v.tif
file_size: 73174939
relation: main_file
success: 1
- access_level: open_access
checksum: 1ee1feea85c8319ebc6c49147502e412
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:46:26Z
date_updated: 2023-08-03T12:46:26Z
file_id: '13642'
file_name: Extdata04_GEPHYRIN_xz.tif
file_size: 36614327
relation: main_file
success: 1
- access_level: open_access
checksum: 64d9d868ef89c5cfc65cb199aff831b4
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:52:23Z
date_updated: 2023-08-03T12:52:23Z
file_id: '13643'
file_name: Extdata06_a-b_n2v.tif
file_size: 2195652677
relation: main_file
success: 1
- access_level: open_access
checksum: df41d97987798715b1f66e6e72be169b
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:51:41Z
date_updated: 2023-08-03T12:51:41Z
file_id: '13644'
file_name: Extdata06_a-b.tif
file_size: 1097875840
relation: main_file
success: 1
- access_level: open_access
checksum: 95a3eaa3b70e964f6871a5765a53659b
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:52:57Z
date_updated: 2023-08-03T12:52:57Z
file_id: '13645'
file_name: Extdata07_01_bottom.tif
file_size: 3564577
relation: main_file
success: 1
- access_level: open_access
checksum: 80b41ad0de73fd61eb67a35262627fac
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:52:57Z
date_updated: 2023-08-03T12:52:57Z
file_id: '13646'
file_name: Extdata07_01_top.tif
file_size: 11958844
relation: main_file
success: 1
- access_level: open_access
checksum: f694a0f11e2f782702cb4c411941873a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:54:27Z
date_updated: 2023-08-03T12:54:27Z
file_id: '13647'
file_name: Extdata07_2_bottom.tif
file_size: 3564581
relation: main_file
success: 1
- access_level: open_access
checksum: b8b1a1339a25806615c0aa7906a032ff
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:54:27Z
date_updated: 2023-08-03T12:54:27Z
file_id: '13648'
file_name: Extdata07_2_top.tif
file_size: 11973592
relation: main_file
success: 1
- access_level: open_access
checksum: 8a24eb623d65d809fe8afbd527528a77
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:54:27Z
date_updated: 2023-08-03T12:54:27Z
file_id: '13649'
file_name: Extdata07_3_bottom.tif
file_size: 1281901
relation: main_file
success: 1
- access_level: open_access
checksum: 816b35def703bc48d55b0293fcd8212f
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:54:27Z
date_updated: 2023-08-03T12:54:27Z
file_id: '13650'
file_name: Extdata07_3_top.tif
file_size: 16569776
relation: main_file
success: 1
- access_level: open_access
checksum: 7f1375ab8cf28cd136e2e44d1fdfd87d
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:54:27Z
date_updated: 2023-08-03T12:54:27Z
file_id: '13651'
file_name: Extdata07_04_bottom.tif
file_size: 3564595
relation: main_file
success: 1
- access_level: open_access
checksum: b1dbda28e2a010968716c0411568f139
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:54:27Z
date_updated: 2023-08-03T12:54:27Z
file_id: '13652'
file_name: Extdata07_04_top.tif
file_size: 5896404
relation: main_file
success: 1
- access_level: open_access
checksum: d6e96b7c8e6a7084c14eda83a4dd778a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:54:28Z
date_updated: 2023-08-03T12:54:28Z
file_id: '13653'
file_name: Extdata07_5_bottom.tif
file_size: 3564609
relation: main_file
success: 1
- access_level: open_access
checksum: a647f89a3048f71512cc58c9e53ec022
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:54:28Z
date_updated: 2023-08-03T12:54:28Z
file_id: '13654'
file_name: Extdata07_5_top.tif
file_size: 15555618
relation: main_file
success: 1
- access_level: open_access
checksum: 66becb046fe591914df572617d720202
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:54:28Z
date_updated: 2023-08-03T12:54:28Z
file_id: '13655'
file_name: Extdata07_6_bottom.tif
file_size: 3564577
relation: main_file
success: 1
- access_level: open_access
checksum: 5d76b2ad7d7acbb1afa27cf52194b1cb
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:54:28Z
date_updated: 2023-08-03T12:54:28Z
file_id: '13656'
file_name: Extdata07_6_top.tif
file_size: 6038186
relation: main_file
success: 1
- access_level: open_access
checksum: 7f137bdea0f0acb3db75c983ce44ab44
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:54:28Z
date_updated: 2023-08-03T12:54:28Z
file_id: '13657'
file_name: Extdata07_7_bottom.tif
file_size: 3560937
relation: main_file
success: 1
- access_level: open_access
checksum: 479e26106280eb61a2d74e29137a2e43
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:54:28Z
date_updated: 2023-08-03T12:54:28Z
file_id: '13658'
file_name: Extdata07_7_top.tif
file_size: 12031850
relation: main_file
success: 1
- access_level: open_access
checksum: 8426197ad283c1bc83e3193f323d5f5a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:54:28Z
date_updated: 2023-08-03T12:54:28Z
file_id: '13659'
file_name: Extdata07_8_bottom.tif
file_size: 3560937
relation: main_file
success: 1
- access_level: open_access
checksum: b9f75a82b564e1c5c363a495d078f6b7
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:54:28Z
date_updated: 2023-08-03T12:54:28Z
file_id: '13660'
file_name: Extdata07_8_top.tif
file_size: 22877176
relation: main_file
success: 1
- access_level: open_access
checksum: 7f1a9f3bb9611362995ae832b2607f4d
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:54:28Z
date_updated: 2023-08-03T12:54:28Z
file_id: '13661'
file_name: Extdata07_9_bottom.tif
file_size: 3564605
relation: main_file
success: 1
- access_level: open_access
checksum: 40286bcb876b655d7421534d0215e86b
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T12:54:28Z
date_updated: 2023-08-03T12:54:28Z
file_id: '13662'
file_name: Extdata07_9_top.tif
file_size: 2744190
relation: main_file
success: 1
- access_level: open_access
checksum: 4af220986cc94d412c2354aa7e6c8cce
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:00:03Z
date_updated: 2023-08-03T13:00:03Z
file_id: '13663'
file_name: Extdata08_6_left.png
file_size: 7243611
relation: main_file
success: 1
- access_level: open_access
checksum: af9c2299f1005a3ccc39f41082cea642
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:00:03Z
date_updated: 2023-08-03T13:00:03Z
file_id: '13664'
file_name: Extdata08_6_right.tif
file_size: 5562487
relation: main_file
success: 1
- access_level: open_access
checksum: 63ef28a174215c70e31d2cabf67d90fc
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:00:04Z
date_updated: 2023-08-03T13:00:04Z
file_id: '13665'
file_name: Extdata08_7_left.tif
file_size: 34745956
relation: main_file
success: 1
- access_level: open_access
checksum: 46de918dfe5f3c23e497ee3daa608dd9
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:00:03Z
date_updated: 2023-08-03T13:00:03Z
file_id: '13666'
file_name: Extdata08_7_right.tif
file_size: 5562483
relation: main_file
success: 1
- access_level: open_access
checksum: 8243a7985d2902446183adc35519a0c8
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:00:04Z
date_updated: 2023-08-03T13:00:04Z
file_id: '13667'
file_name: Extdata08_b_1_left.tif
file_size: 31506150
relation: main_file
success: 1
- access_level: open_access
checksum: 857cffa31569efcd5917f9802a106d58
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:00:04Z
date_updated: 2023-08-03T13:00:04Z
file_id: '13668'
file_name: Extdata08_b_1_right.tif
file_size: 11117705
relation: main_file
success: 1
- access_level: open_access
checksum: e34902de9119d2cd9f1910f610627c5f
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:00:04Z
date_updated: 2023-08-03T13:00:04Z
file_id: '13669'
file_name: Extdata08_b_2_left.png
file_size: 3540493
relation: main_file
success: 1
- access_level: open_access
checksum: 1247d636ac5af22e8950ffd2ff213fea
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:00:05Z
date_updated: 2023-08-03T13:00:05Z
file_id: '13670'
file_name: Extdata08_b_2_right.tif
file_size: 5562553
relation: main_file
success: 1
- access_level: open_access
checksum: f47c2d3f6824e08853ae2631f6ee7c5f
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:00:05Z
date_updated: 2023-08-03T13:00:05Z
file_id: '13671'
file_name: Extdata08_b_3_left.png
file_size: 3541547
relation: main_file
success: 1
- access_level: open_access
checksum: 1247d636ac5af22e8950ffd2ff213fea
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:00:05Z
date_updated: 2023-08-03T13:00:05Z
file_id: '13672'
file_name: Extdata08_b_3_right.tif
file_size: 5562553
relation: main_file
success: 1
- access_level: open_access
checksum: e90a95ec902a683678acd70336852fd9
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:00:05Z
date_updated: 2023-08-03T13:00:05Z
file_id: '13673'
file_name: Extdata08_b_4_left.tif
file_size: 25819844
relation: main_file
success: 1
- access_level: open_access
checksum: 94cd44cd1c8c7a87ffd5861bf88561a1
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:00:05Z
date_updated: 2023-08-03T13:00:05Z
file_id: '13674'
file_name: Extdata08_b_4_right.tif
file_size: 5561131
relation: main_file
success: 1
- access_level: open_access
checksum: fd4ca56dc5c48ad4546df22daef04efc
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:00:06Z
date_updated: 2023-08-03T13:00:06Z
file_id: '13675'
file_name: Extdata08_b_5_left.tif
file_size: 38786287
relation: main_file
success: 1
- access_level: open_access
checksum: 52c04890160c4e0f23d3fe2d3ed0c8e7
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:00:06Z
date_updated: 2023-08-03T13:00:06Z
file_id: '13676'
file_name: Extdata08_b_5_right.tif
file_size: 5562487
relation: main_file
success: 1
- access_level: open_access
checksum: 47ee6f4b083130d5bb720bcf8a832443
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:04:52Z
date_updated: 2023-08-03T13:04:52Z
file_id: '13677'
file_name: Extdata08_a_1-1.png
file_size: 2133527
relation: main_file
success: 1
- access_level: open_access
checksum: 5ca2279c372b3b7dbce3ea5658a68399
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:04:52Z
date_updated: 2023-08-03T13:04:52Z
file_id: '13678'
file_name: Extdata08_a_1-2.png
file_size: 1782607
relation: main_file
success: 1
- access_level: open_access
checksum: 34b7781e3e4abf9f30a0c85db156b6d5
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:04:52Z
date_updated: 2023-08-03T13:04:52Z
file_id: '13679'
file_name: Extdata08_a_2.png
file_size: 5117469
relation: main_file
success: 1
- access_level: open_access
checksum: 25717007393682098d2208bd0676a0f7
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:04:52Z
date_updated: 2023-08-03T13:04:52Z
file_id: '13680'
file_name: Extdata08_a_3.png
file_size: 6911658
relation: main_file
success: 1
- access_level: open_access
checksum: b4b003809acec33c71628066744c7ea9
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:04:53Z
date_updated: 2023-08-03T13:04:53Z
file_id: '13681'
file_name: Extdata08_a_4.png
file_size: 8579427
relation: main_file
success: 1
- access_level: open_access
checksum: 4410976cf867ecacf8f197dbd28cc5a0
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:04:53Z
date_updated: 2023-08-03T13:04:53Z
file_id: '13682'
file_name: Extdata08_a_5.png
file_size: 9675065
relation: main_file
success: 1
- access_level: open_access
checksum: a235dd204f28d3323883e044b031b984
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:04:53Z
date_updated: 2023-08-03T13:04:53Z
file_id: '13683'
file_name: Extdata08_a_6.png
file_size: 9769588
relation: main_file
success: 1
- access_level: open_access
checksum: 0e2b768e4eb7200cf6fe28ba511a5ef6
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:04:53Z
date_updated: 2023-08-03T13:04:53Z
file_id: '13684'
file_name: Extdata08_a_7.png
file_size: 9114791
relation: main_file
success: 1
- access_level: open_access
checksum: df4a01c62233ee8ed601c4b90d7780d0
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:04:57Z
date_updated: 2023-08-03T13:04:57Z
file_id: '13685'
file_name: Extdata08_a_8.png
file_size: 124540688
relation: main_file
success: 1
- access_level: open_access
checksum: 1f407c7915e2717a56fec258708e7b7a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:11:30Z
date_updated: 2023-08-03T13:11:30Z
file_id: '13686'
file_name: Exdata09_c_n2v.tif
file_size: 204861336
relation: main_file
success: 1
- access_level: open_access
checksum: 9089dc7c9914edf9750eebd65819708d
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:11:26Z
date_updated: 2023-08-03T13:11:26Z
file_id: '13687'
file_name: Extdata09_a_zoom.tif
file_size: 252149
relation: main_file
success: 1
- access_level: open_access
checksum: 62d295a62cb8dd36c268b28cf0251322
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:11:26Z
date_updated: 2023-08-03T13:11:26Z
file_id: '13688'
file_name: Extdata09_a.tif
file_size: 11117705
relation: main_file
success: 1
- access_level: open_access
checksum: e874d8ee287f924c1076134b5b06bdb2
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:11:31Z
date_updated: 2023-08-03T13:11:31Z
file_id: '13689'
file_name: Extdata09_b_n2v.tif
file_size: 256074660
relation: main_file
success: 1
- access_level: open_access
checksum: 488068fff7b42bcf5728739e2a06d742
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:12:53Z
date_updated: 2023-08-03T13:12:53Z
file_id: '13690'
file_name: Extdata10_n2v_CLAHE.tif
file_size: 127914854
relation: main_file
success: 1
- access_level: open_access
checksum: 35351f5af21ce854d41577813a7bdb57
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:21:35Z
date_updated: 2023-08-03T13:21:35Z
file_id: '13691'
file_name: Supplfig01_a_1.png
file_size: 430878
relation: main_file
success: 1
- access_level: open_access
checksum: 36204e9e604206366ffc6f61ec91915b
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:21:35Z
date_updated: 2023-08-03T13:21:35Z
file_id: '13692'
file_name: Supplfig01_a_2.png
file_size: 438843
relation: main_file
success: 1
- access_level: open_access
checksum: 92ccc934c4603b29f4f7a96573e49c83
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:21:35Z
date_updated: 2023-08-03T13:21:35Z
file_id: '13693'
file_name: Supplfig01_a_3.png
file_size: 426713
relation: main_file
success: 1
- access_level: open_access
checksum: e71faa54934bee8ac3e66ce692f542ab
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:21:35Z
date_updated: 2023-08-03T13:21:35Z
file_id: '13694'
file_name: Supplfig01_a_4.tif
file_size: 723221
relation: main_file
success: 1
- access_level: open_access
checksum: 95dc33563f48b650e5ac020bdfd10c48
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:21:35Z
date_updated: 2023-08-03T13:21:35Z
file_id: '13695'
file_name: Supplfig01_a_5.tif
file_size: 723235
relation: main_file
success: 1
- access_level: open_access
checksum: c62df0a9faf1c47af121d77eb1582edd
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:21:35Z
date_updated: 2023-08-03T13:21:35Z
file_id: '13696'
file_name: Supplfig01_b_1.tif
file_size: 1442129
relation: main_file
success: 1
- access_level: open_access
checksum: 8ae157c5a4a543da1193cb8ca12b1919
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:21:35Z
date_updated: 2023-08-03T13:21:35Z
file_id: '13697'
file_name: Supplfig01_b_2.tif
file_size: 725407
relation: main_file
success: 1
- access_level: open_access
checksum: 388370d954ce842781c0d62edfb448a7
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:21:35Z
date_updated: 2023-08-03T13:21:35Z
file_id: '13698'
file_name: Supplfig01_c_1.tif
file_size: 721641
relation: main_file
success: 1
- access_level: open_access
checksum: 6cae58ba3c98bc8253e1c769e93657a7
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:21:35Z
date_updated: 2023-08-03T13:21:35Z
file_id: '13699'
file_name: Supplfig01_c_2.tif
file_size: 6003079
relation: main_file
success: 1
- access_level: open_access
checksum: 85cf013232c72895aefc07f5411a765a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:21:35Z
date_updated: 2023-08-03T13:21:35Z
file_id: '13700'
file_name: Supplfig01_c_3.tif
file_size: 2003311
relation: main_file
success: 1
- access_level: open_access
checksum: dfb33b91066e3f34d3803f59a4105aba
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:21:35Z
date_updated: 2023-08-03T13:21:35Z
file_id: '13701'
file_name: Supplfig01_c_4.tif
file_size: 723271
relation: main_file
success: 1
- access_level: open_access
checksum: 8b899826fe48d7e7536b0b02a2645e87
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:21:35Z
date_updated: 2023-08-03T13:21:35Z
file_id: '13702'
file_name: Supplfig01_d_1.tif
file_size: 721901
relation: main_file
success: 1
- access_level: open_access
checksum: 67529fa6e0f12c272275e823f6045f6d
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:21:35Z
date_updated: 2023-08-03T13:21:35Z
file_id: '13703'
file_name: Supplfig01_d_2.tif
file_size: 721901
relation: main_file
success: 1
- access_level: open_access
checksum: 111a9231f63c775340d3356109715cb0
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:21:35Z
date_updated: 2023-08-03T13:21:35Z
file_id: '13704'
file_name: Supplfig01_e_1.tif
file_size: 723223
relation: main_file
success: 1
- access_level: open_access
checksum: 7685bf02408dcb095a00854ff22763ed
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:21:35Z
date_updated: 2023-08-03T13:21:35Z
file_id: '13705'
file_name: Supplfig01_e_2.tif
file_size: 723223
relation: main_file
success: 1
- access_level: open_access
checksum: 455f5cb9aa8a87b91586a27aca122610
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:26:00Z
date_updated: 2023-08-03T13:26:00Z
file_id: '13706'
file_name: Supplfig02_b_01.tif
file_size: 34821298
relation: main_file
success: 1
- access_level: open_access
checksum: 311545b39aaa2c72755fd8ae190803a5
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:26:00Z
date_updated: 2023-08-03T13:26:00Z
file_id: '13707'
file_name: Supplfig02_b_02.tif
file_size: 32131123
relation: main_file
success: 1
- access_level: open_access
checksum: 682deb61f57d9ca54716160ddd52a6cf
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:26:01Z
date_updated: 2023-08-03T13:26:01Z
file_id: '13708'
file_name: Supplfig02_b_03.tif
file_size: 36872674
relation: main_file
success: 1
- access_level: open_access
checksum: 4f9579c91b2a3f36ff6e66b592907973
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:26:01Z
date_updated: 2023-08-03T13:26:01Z
file_id: '13709'
file_name: Supplfig02_b_04.tif
file_size: 39964115
relation: main_file
success: 1
- access_level: open_access
checksum: 7ae7053673b53dbed59ffb92618f969a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:26:02Z
date_updated: 2023-08-03T13:26:02Z
file_id: '13710'
file_name: Supplfig02_b_05.tif
file_size: 43053091
relation: main_file
success: 1
- access_level: open_access
checksum: 827b26d3d2b6ddc9bced9d6113023ced
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:26:02Z
date_updated: 2023-08-03T13:26:02Z
file_id: '13711'
file_name: Supplfig02_b_06.tif
file_size: 46998147
relation: main_file
success: 1
- access_level: open_access
checksum: bc2e7e8badb42f8e1facc8b7bc96fdda
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:26:03Z
date_updated: 2023-08-03T13:26:03Z
file_id: '13712'
file_name: Supplfig02_b_07.tif
file_size: 45179219
relation: main_file
success: 1
- access_level: open_access
checksum: c2b78aa0f433928afa10a743c5fb73b8
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:26:03Z
date_updated: 2023-08-03T13:26:03Z
file_id: '13713'
file_name: Supplfig02_b_08.tif
file_size: 46978802
relation: main_file
success: 1
- access_level: open_access
checksum: 9cd1ab61cced2c0981e813aeae930855
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:26:04Z
date_updated: 2023-08-03T13:26:04Z
file_id: '13714'
file_name: Supplfig02_b_09.tif
file_size: 48840915
relation: main_file
success: 1
- access_level: open_access
checksum: 3086126338aee6ec4ebc1d3350551197
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:26:04Z
date_updated: 2023-08-03T13:26:04Z
file_id: '13715'
file_name: Supplfig02_b_10.tif
file_size: 45907059
relation: main_file
success: 1
- access_level: open_access
checksum: 69f52c2b3bb5b5f9fef057860b3386fa
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:26:05Z
date_updated: 2023-08-03T13:26:05Z
file_id: '13716'
file_name: Supplfig02_b_11.tif
file_size: 32231538
relation: main_file
success: 1
- access_level: open_access
checksum: defdbeab1e672456bfeafdd1983e779e
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:26:05Z
date_updated: 2023-08-03T13:26:05Z
file_id: '13717'
file_name: Supplfig02_b_12.tif
file_size: 35264963
relation: main_file
success: 1
- access_level: open_access
checksum: 02c566f781b9ad44f04ce0088a1fa27a
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:28:40Z
date_updated: 2023-08-03T13:28:40Z
file_id: '13718'
file_name: Supplfig03_a_std_xy.png
file_size: 63326
relation: main_file
success: 1
- access_level: open_access
checksum: c6c3630ee9a6ed5d207cb68e330da566
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:28:40Z
date_updated: 2023-08-03T13:28:40Z
file_id: '13719'
file_name: Supplfig03_a_std_xz.png
file_size: 50211
relation: main_file
success: 1
- access_level: open_access
checksum: 0dcd4d19e83a7ff259d6aa04d746cd90
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:28:41Z
date_updated: 2023-08-03T13:28:41Z
file_id: '13720'
file_name: Supplfig03_b_std_xy.png
file_size: 52629
relation: main_file
success: 1
- access_level: open_access
checksum: 4785de4a6dcb23b25a06b0da29df5bb5
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:28:41Z
date_updated: 2023-08-03T13:28:41Z
file_id: '13721'
file_name: Supplfig03_b_std_xz.png
file_size: 72245
relation: main_file
success: 1
- access_level: open_access
checksum: 19d0f198bd8a25073e45e7950d7a78c2
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:28:45Z
date_updated: 2023-08-03T13:28:45Z
file_id: '13722'
file_name: Supplfig03_model1.tif
file_size: 98570368
relation: main_file
success: 1
- access_level: open_access
checksum: 51ff687df4a0867f39b69c2c7a4ee3ca
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:28:45Z
date_updated: 2023-08-03T13:28:45Z
file_id: '13723'
file_name: Supplfig03_model2.tif
file_size: 98570367
relation: main_file
success: 1
- access_level: open_access
checksum: ec9d9a041ca85fe632c612baf981fcc5
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:28:49Z
date_updated: 2023-08-03T13:28:49Z
file_id: '13724'
file_name: Supplfig03_model3.tif
file_size: 98570365
relation: main_file
success: 1
- access_level: open_access
checksum: a988b15a9529094f1b5519a72871e98e
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:28:49Z
date_updated: 2023-08-03T13:28:49Z
file_id: '13725'
file_name: Supplfig03_model4.tif
file_size: 98570365
relation: main_file
success: 1
- access_level: open_access
checksum: 4a4fe88fcfd87fcfe118a5a73d81d6b0
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:28:52Z
date_updated: 2023-08-03T13:28:52Z
file_id: '13726'
file_name: Supplfig03_model5.tif
file_size: 98570369
relation: main_file
success: 1
- access_level: open_access
checksum: ce9b774ff5163ebfa4e3fb162f4cfffe
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:34:27Z
date_updated: 2023-08-03T13:34:27Z
file_id: '13727'
file_name: Supplfig04_a_n2v.tif
file_size: 1036927887
relation: main_file
success: 1
- access_level: open_access
checksum: 21f1bb8d2a00c88bdd6febe6285acc2a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:34:13Z
date_updated: 2023-08-03T13:34:13Z
file_id: '13728'
file_name: Supplfig04_a.tif
file_size: 518519275
relation: main_file
success: 1
- access_level: open_access
checksum: 503820214ab994c01b18a241f3b62434
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:34:22Z
date_updated: 2023-08-03T13:34:22Z
file_id: '13729'
file_name: Supplfig04_b.tif
file_size: 158440069
relation: main_file
success: 1
- access_level: open_access
checksum: e0469102a87332b46904b4ba35b8fb2f
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:34:37Z
date_updated: 2023-08-03T13:34:37Z
file_id: '13730'
file_name: Supplfig04_c_n2v.tif
file_size: 309407894
relation: main_file
success: 1
- access_level: open_access
checksum: c30a0d18c43045a8fbc5501a37061bd2
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:34:38Z
date_updated: 2023-08-03T13:34:38Z
file_id: '13731'
file_name: Supplfig04_c.tif
file_size: 154728887
relation: main_file
success: 1
- access_level: open_access
checksum: 69c706710d642b30cda6951494702139
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:34:43Z
date_updated: 2023-08-03T13:34:43Z
file_id: '13732'
file_name: Supplfig04_d_n2v.tif
file_size: 106696749
relation: main_file
success: 1
- access_level: open_access
checksum: 7dbd2e881ebf049352913188fa10224a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:34:41Z
date_updated: 2023-08-03T13:34:41Z
file_id: '13733'
file_name: Supplfig04_d.tif
file_size: 53367343
relation: main_file
success: 1
- access_level: open_access
checksum: fd0d1ec674dabbb499fab383b7eb4d58
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:34:46Z
date_updated: 2023-08-03T13:34:46Z
file_id: '13734'
file_name: Supplfig04_e_n2v.tiff
file_size: 127917734
relation: main_file
success: 1
- access_level: open_access
checksum: 86de309d9cd434afbe371da283d8adb6
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:34:46Z
date_updated: 2023-08-03T13:34:46Z
file_id: '13735'
file_name: Supplfig04_e.tif
file_size: 63974204
relation: main_file
success: 1
- access_level: open_access
checksum: ada99b2b5900cb198d1de3cc9e37ebd1
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:37Z
date_updated: 2023-08-03T13:35:37Z
file_id: '13736'
file_name: Supplfig05_a_xy_1.png
file_size: 8403
relation: main_file
success: 1
- access_level: open_access
checksum: f2e382925a972b98560614016ae3bc55
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:37Z
date_updated: 2023-08-03T13:35:37Z
file_id: '13737'
file_name: Supplfig05_a_xy_2.png
file_size: 10087
relation: main_file
success: 1
- access_level: open_access
checksum: ef22b640f33d677add1a7b5153f95420
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:37Z
date_updated: 2023-08-03T13:35:37Z
file_id: '13738'
file_name: Supplfig05_a_xy_3.png
file_size: 24561
relation: main_file
success: 1
- access_level: open_access
checksum: b854cc4a5224b8a6f5d59c74f5740ccd
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:37Z
date_updated: 2023-08-03T13:35:37Z
file_id: '13739'
file_name: Supplfig05_a_xy_4.png
file_size: 8353
relation: main_file
success: 1
- access_level: open_access
checksum: 446b0362c5ea6e2a5f0e1f8359422358
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:37Z
date_updated: 2023-08-03T13:35:37Z
file_id: '13740'
file_name: Supplfig05_a_xy_5.png
file_size: 8337
relation: main_file
success: 1
- access_level: open_access
checksum: 5585f7b62fa09ed5a63090b679383601
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:37Z
date_updated: 2023-08-03T13:35:37Z
file_id: '13741'
file_name: Supplfig05_a_xz_1.png
file_size: 7399
relation: main_file
success: 1
- access_level: open_access
checksum: 190d88bcacbd11d41f4a0e2bbd140862
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:37Z
date_updated: 2023-08-03T13:35:37Z
file_id: '13742'
file_name: Supplfig05_a_xz_2.png
file_size: 10887
relation: main_file
success: 1
- access_level: open_access
checksum: 0c89010651862f21032a303e159b7a1c
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:37Z
date_updated: 2023-08-03T13:35:37Z
file_id: '13743'
file_name: Supplfig05_a_xz_3.png
file_size: 21654
relation: main_file
success: 1
- access_level: open_access
checksum: 94e61e553aa2280721acce7d407ff24a
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:37Z
date_updated: 2023-08-03T13:35:37Z
file_id: '13744'
file_name: Supplfig05_a_xz_4.png
file_size: 7248
relation: main_file
success: 1
- access_level: open_access
checksum: ef9157274686ff5d7056f5a519d413dd
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:37Z
date_updated: 2023-08-03T13:35:37Z
file_id: '13745'
file_name: Supplfig05_a_xz_5.png
file_size: 7214
relation: main_file
success: 1
- access_level: open_access
checksum: 4b389473cbf769e6e34670dae2224af1
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:37Z
date_updated: 2023-08-03T13:35:37Z
file_id: '13746'
file_name: Supplfig05_b_xy_1.png
file_size: 8544
relation: main_file
success: 1
- access_level: open_access
checksum: b05aa1e201758a5d55e35d719452fafc
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:37Z
date_updated: 2023-08-03T13:35:37Z
file_id: '13747'
file_name: Supplfig05_b_xy_2.png
file_size: 11135
relation: main_file
success: 1
- access_level: open_access
checksum: 890f3516930251a367066c049141d6bb
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:37Z
date_updated: 2023-08-03T13:35:37Z
file_id: '13748'
file_name: Supplfig05_b_xy_3.png
file_size: 26762
relation: main_file
success: 1
- access_level: open_access
checksum: 36345c1d582758ff463072fd04578c0c
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:37Z
date_updated: 2023-08-03T13:35:37Z
file_id: '13749'
file_name: Supplfig05_b_xy_4.png
file_size: 8438
relation: main_file
success: 1
- access_level: open_access
checksum: c9499ad8914c405a07fc4469b19e8987
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:37Z
date_updated: 2023-08-03T13:35:37Z
file_id: '13750'
file_name: Supplfig05_b_xy_5.png
file_size: 8434
relation: main_file
success: 1
- access_level: open_access
checksum: 7e7a7c900ce1fc3187ab648f207f9425
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:37Z
date_updated: 2023-08-03T13:35:37Z
file_id: '13751'
file_name: Supplfig05_b_xz_1.png
file_size: 8269
relation: main_file
success: 1
- access_level: open_access
checksum: b645d1e2422ef3f05344ccd3f955468d
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:37Z
date_updated: 2023-08-03T13:35:37Z
file_id: '13752'
file_name: Supplfig05_b_xz_2.png
file_size: 12762
relation: main_file
success: 1
- access_level: open_access
checksum: ef0b83b49cf47ca7d8ffe3bdc609fe38
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:37Z
date_updated: 2023-08-03T13:35:37Z
file_id: '13753'
file_name: Supplfig05_b_xz_3.png
file_size: 24819
relation: main_file
success: 1
- access_level: open_access
checksum: 68719d4d464a2ffc198cb6ad070c4579
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:37Z
date_updated: 2023-08-03T13:35:37Z
file_id: '13754'
file_name: Supplfig05_b_xz_4.png
file_size: 8126
relation: main_file
success: 1
- access_level: open_access
checksum: 18370f47177ef37a477c6fd7bda3fea0
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:38Z
date_updated: 2023-08-03T13:35:38Z
file_id: '13755'
file_name: Supplfig05_b_xz_5.png
file_size: 8114
relation: main_file
success: 1
- access_level: open_access
checksum: 013d4bd9704ace490810b9ef21b12454
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:38Z
date_updated: 2023-08-03T13:35:38Z
file_id: '13756'
file_name: Supplfig05_c_xy_1.png
file_size: 8273
relation: main_file
success: 1
- access_level: open_access
checksum: bb431f827386e79c54452d6284f501b9
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:38Z
date_updated: 2023-08-03T13:35:38Z
file_id: '13757'
file_name: Supplfig05_c_xy_2.png
file_size: 12639
relation: main_file
success: 1
- access_level: open_access
checksum: 4cd54f58c5afff88832a508655f913bb
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:38Z
date_updated: 2023-08-03T13:35:38Z
file_id: '13758'
file_name: Supplfig05_c_xy_3.png
file_size: 25288
relation: main_file
success: 1
- access_level: open_access
checksum: 07f86f98f6a65eac8608cec8c567309e
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:38Z
date_updated: 2023-08-03T13:35:38Z
file_id: '13759'
file_name: Supplfig05_c_xz_1.png
file_size: 6786
relation: main_file
success: 1
- access_level: open_access
checksum: 492f699b71761214272b17432e3a54fa
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:38Z
date_updated: 2023-08-03T13:35:38Z
file_id: '13760'
file_name: Supplfig05_c_xz_2.png
file_size: 9781
relation: main_file
success: 1
- access_level: open_access
checksum: af617da5638db849bea6ff51a08227c6
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:38Z
date_updated: 2023-08-03T13:35:38Z
file_id: '13761'
file_name: Supplfig05_c_xz_3.png
file_size: 20728
relation: main_file
success: 1
- access_level: open_access
checksum: 5e5d5694af4a22297046d697a01fa4d6
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:38Z
date_updated: 2023-08-03T13:35:38Z
file_id: '13762'
file_name: Supplfig05_d_xy_1.png
file_size: 8428
relation: main_file
success: 1
- access_level: open_access
checksum: 38bf7b75f3dedd0a3722faae05aa0233
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:38Z
date_updated: 2023-08-03T13:35:38Z
file_id: '13763'
file_name: Supplfig05_d_xy_2.png
file_size: 10813
relation: main_file
success: 1
- access_level: open_access
checksum: e4ea966553f158ec2cfbdc696780390e
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:38Z
date_updated: 2023-08-03T13:35:38Z
file_id: '13764'
file_name: Supplfig05_d_xy_3.png
file_size: 26552
relation: main_file
success: 1
- access_level: open_access
checksum: 356f424d9f5f608e9aea962bb63672f1
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:38Z
date_updated: 2023-08-03T13:35:38Z
file_id: '13765'
file_name: Supplfig05_d_xz_1.png
file_size: 7876
relation: main_file
success: 1
- access_level: open_access
checksum: 05068476beee0f394e28b98209686394
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:38Z
date_updated: 2023-08-03T13:35:38Z
file_id: '13766'
file_name: Supplfig05_d_xz_2.png
file_size: 11634
relation: main_file
success: 1
- access_level: open_access
checksum: 78b3a0d93df22383a384a2f3acc80401
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:38Z
date_updated: 2023-08-03T13:35:38Z
file_id: '13767'
file_name: Supplfig05_d_xz_3.png
file_size: 25006
relation: main_file
success: 1
- access_level: open_access
checksum: f5ee6a817f11a42c9db82ad6325f58b9
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:38Z
date_updated: 2023-08-03T13:35:38Z
file_id: '13768'
file_name: Supplfig05_e_xy_1.png
file_size: 7895
relation: main_file
success: 1
- access_level: open_access
checksum: 019ca89e892d6e19589609bc99eab331
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:38Z
date_updated: 2023-08-03T13:35:38Z
file_id: '13769'
file_name: Supplfig05_e_xy_2.png
file_size: 6180
relation: main_file
success: 1
- access_level: open_access
checksum: 0e1a5cbbd70dc245edaecaca70d974cb
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:38Z
date_updated: 2023-08-03T13:35:38Z
file_id: '13770'
file_name: Supplfig05_e_xy_3.png
file_size: 18326
relation: main_file
success: 1
- access_level: open_access
checksum: 4d4d64a5bb7b096aaf8130ece4512bc2
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:38Z
date_updated: 2023-08-03T13:35:38Z
file_id: '13771'
file_name: Supplfig05_e_xz_1.png
file_size: 6295
relation: main_file
success: 1
- access_level: open_access
checksum: 607302aec5ec2a14a271dbffd3465261
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:38Z
date_updated: 2023-08-03T13:35:38Z
file_id: '13772'
file_name: Supplfig05_e_xz_2.png
file_size: 4335
relation: main_file
success: 1
- access_level: open_access
checksum: a673e17ead1fd2e776b9a5f560a289ab
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T13:35:38Z
date_updated: 2023-08-03T13:35:38Z
file_id: '13773'
file_name: Supplfig05_e_xz_3.png
file_size: 23243
relation: main_file
success: 1
- access_level: open_access
checksum: 4349e511fc86a8bddb790a7be7175c75
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:46:13Z
date_updated: 2023-08-03T13:46:13Z
file_id: '13774'
file_name: Supplfig06_a_1.tif
file_size: 4002153
relation: main_file
success: 1
- access_level: open_access
checksum: cd12977e4c1a472d80193be01f69a895
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:46:13Z
date_updated: 2023-08-03T13:46:13Z
file_id: '13775'
file_name: Supplfig06_a_2.tif
file_size: 2252137
relation: main_file
success: 1
- access_level: open_access
checksum: 575e32b8c25ce658e20f0c86a5f1b9b7
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:46:13Z
date_updated: 2023-08-03T13:46:13Z
file_id: '13776'
file_name: Supplfig06_a_3.tif
file_size: 2252137
relation: main_file
success: 1
- access_level: open_access
checksum: b06f775e0210e7a16cb4b58aed32118c
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:46:13Z
date_updated: 2023-08-03T13:46:13Z
file_id: '13777'
file_name: Supplfig06_a_i.tif
file_size: 78329
relation: main_file
success: 1
- access_level: open_access
checksum: 56854c1a05c721c36877fe7c7454b7d7
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:46:13Z
date_updated: 2023-08-03T13:46:13Z
file_id: '13778'
file_name: Supplfig06_a_ii.tif
file_size: 78329
relation: main_file
success: 1
- access_level: open_access
checksum: 9aff42ea5138931fedb5a00643ae7a22
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:46:13Z
date_updated: 2023-08-03T13:46:13Z
file_id: '13779'
file_name: Supplfig06_a_iii.tif
file_size: 78329
relation: main_file
success: 1
- access_level: open_access
checksum: 9474b7d00d4dee46659afedef75dc095
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:46:13Z
date_updated: 2023-08-03T13:46:13Z
file_id: '13780'
file_name: Supplfig06_d_1.tif
file_size: 501149
relation: main_file
success: 1
- access_level: open_access
checksum: 53df41dd968b04b4657cd2b91870d636
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:46:13Z
date_updated: 2023-08-03T13:46:13Z
file_id: '13781'
file_name: Supplfig06_d_2.tif
file_size: 500153
relation: main_file
success: 1
- access_level: open_access
checksum: 1a39b8310598dd25d97f848b682b8de0
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:46:14Z
date_updated: 2023-08-03T13:46:14Z
file_id: '13782'
file_name: Supplfig06_d_3.tif
file_size: 500153
relation: main_file
success: 1
- access_level: open_access
checksum: 6d9b496be6aecedbb2cb4cbf68f02872
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:46:14Z
date_updated: 2023-08-03T13:46:14Z
file_id: '13783'
file_name: Supplfig06_d_i.tif
file_size: 78329
relation: main_file
success: 1
- access_level: open_access
checksum: bc034b1636c2db8b9d9e7bd131b9780a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:46:14Z
date_updated: 2023-08-03T13:46:14Z
file_id: '13784'
file_name: Supplfig06_d_ii.tif
file_size: 78329
relation: main_file
success: 1
- access_level: open_access
checksum: 54f6e55e30a2aeea6c82eb6dbd376240
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:46:14Z
date_updated: 2023-08-03T13:46:14Z
file_id: '13785'
file_name: Supplfig06_d_iii.tif
file_size: 78329
relation: main_file
success: 1
- access_level: open_access
checksum: 446da3cc16897277890f23f966c2bf52
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:46:14Z
date_updated: 2023-08-03T13:46:14Z
file_id: '13786'
file_name: Supplfig06_g_1.tif
file_size: 960011
relation: main_file
success: 1
- access_level: open_access
checksum: 71192d0ec592a33195f2386a5f95aa86
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:46:14Z
date_updated: 2023-08-03T13:46:14Z
file_id: '13787'
file_name: Supplfig06_g_2.tif
file_size: 960011
relation: main_file
success: 1
- access_level: open_access
checksum: 246782dbe8ff525aea360efc72e65f07
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:46:14Z
date_updated: 2023-08-03T13:46:14Z
file_id: '13788'
file_name: Supplfig06_g_3.tif
file_size: 960041
relation: main_file
success: 1
- access_level: open_access
checksum: d49b12fec5508aa8d72587757cccc0a4
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:46:14Z
date_updated: 2023-08-03T13:46:14Z
file_id: '13789'
file_name: Supplfig06_g_4.tif
file_size: 960041
relation: main_file
success: 1
- access_level: open_access
checksum: 27b11b1d4fc784a49ffd7660624d00b6
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:46:14Z
date_updated: 2023-08-03T13:46:14Z
file_id: '13790'
file_name: Supplfig06_g_5.tif
file_size: 963241
relation: main_file
success: 1
- access_level: open_access
checksum: 9ce944049c1b88fadbf43609385f9e2f
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:51:38Z
date_updated: 2023-08-03T13:51:38Z
file_id: '13791'
file_name: Suppl08_a_1.tiff
file_size: 19286002
relation: main_file
success: 1
- access_level: open_access
checksum: 06265515f06688843a415362f7b2c485
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:51:38Z
date_updated: 2023-08-03T13:51:38Z
file_id: '13792'
file_name: Suppl08_a_2.tiff
file_size: 19286002
relation: main_file
success: 1
- access_level: open_access
checksum: 50f0969c6c2c1209ff776bec4861030a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:51:38Z
date_updated: 2023-08-03T13:51:38Z
file_id: '13793'
file_name: Suppl08_a_3.tif
file_size: 617552
relation: main_file
success: 1
- access_level: open_access
checksum: b90965d20133fea73c99050c32eb7695
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:51:38Z
date_updated: 2023-08-03T13:51:38Z
file_id: '13794'
file_name: Suppl08_a_4.tif
file_size: 617552
relation: main_file
success: 1
- access_level: open_access
checksum: 568a1e0efaa62527b51e66a7aabfd05c
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:51:40Z
date_updated: 2023-08-03T13:51:40Z
file_id: '13795'
file_name: Suppl08_a_coCATS_N2V.tif
file_size: 19287339
relation: main_file
success: 1
- access_level: open_access
checksum: 06265515f06688843a415362f7b2c485
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:51:40Z
date_updated: 2023-08-03T13:51:40Z
file_id: '13796'
file_name: Suppl08_c_1_4_predictedBASSOON.tiff
file_size: 19286002
relation: main_file
success: 1
- access_level: open_access
checksum: 568a1e0efaa62527b51e66a7aabfd05c
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:51:43Z
date_updated: 2023-08-03T13:51:43Z
file_id: '13797'
file_name: Suppl08_c_1_coCATS.tif
file_size: 19287339
relation: main_file
success: 1
- access_level: open_access
checksum: feae08c38b1d9c540b697977cd7f2666
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T15:36:47Z
date_updated: 2023-08-03T15:36:47Z
file_id: '13798'
file_name: Suppl08_c_2_coCATS.tif
file_size: 19287340
relation: main_file
success: 1
- access_level: open_access
checksum: af2c1199fdcaecfec76189dae86cc12e
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:51:44Z
date_updated: 2023-08-03T13:51:44Z
file_id: '13799'
file_name: Suppl08_c_2-3_predictedBASSOON.tiff
file_size: 19286002
relation: main_file
success: 1
- access_level: open_access
checksum: 1f92db47605959d0db01f612f7c9c034
content_type: application/octet-stream
creator: jdanzl
date_created: 2023-08-03T13:51:43Z
date_updated: 2023-08-03T13:51:43Z
file_id: '13800'
file_name: Suppl08_c_3-6_roi.roi
file_size: 156
relation: main_file
success: 1
- access_level: open_access
checksum: 4fc4f8803adcfacd53a41b91f4699dc5
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:51:45Z
date_updated: 2023-08-03T13:51:45Z
file_id: '13801'
file_name: Suppl08_e_1_3.tiff
file_size: 19286002
relation: main_file
success: 1
- access_level: open_access
checksum: 459551aae114daa4f5ca5d06fcd6b203
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:51:45Z
date_updated: 2023-08-03T13:51:45Z
file_id: '13802'
file_name: Suppl08_e_1-2.tiff
file_size: 19286002
relation: main_file
success: 1
- access_level: open_access
checksum: 032cbace7ec436f7b62478c0ffd35e5e
content_type: application/octet-stream
creator: jdanzl
date_created: 2023-08-03T13:51:45Z
date_updated: 2023-08-03T13:51:45Z
file_id: '13803'
file_name: Suppl08_e_2-5_roi.roi
file_size: 152
relation: main_file
success: 1
- access_level: open_access
checksum: 7379168be281ef618b8cecd8efd31ed5
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:51:46Z
date_updated: 2023-08-03T13:51:46Z
file_id: '13804'
file_name: Suppl08_e_4-5_coCATS.tiff
file_size: 19286002
relation: main_file
success: 1
- access_level: open_access
checksum: 5c4c092f608f4c10bb422c9e08b5955b
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:51:46Z
date_updated: 2023-08-03T13:51:46Z
file_id: '13805'
file_name: Suppl08_e_4.tif
file_size: 617552
relation: main_file
success: 1
- access_level: open_access
checksum: 903e31be124f6e4ec1c2e7ab7db80e6b
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:51:46Z
date_updated: 2023-08-03T13:51:46Z
file_id: '13806'
file_name: Suppl08_e_5.tif
file_size: 617552
relation: main_file
success: 1
- access_level: open_access
checksum: 173d956392d67b0a7e544629b51881e4
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:51:48Z
date_updated: 2023-08-03T13:51:48Z
file_id: '13807'
file_name: Suppl08_e_6_8.tiff
file_size: 19286002
relation: main_file
success: 1
- access_level: open_access
checksum: 2a4cea867a253a6af7e9b0d2e100de14
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:51:48Z
date_updated: 2023-08-03T13:51:48Z
file_id: '13808'
file_name: Suppl08_e_6-7.tiff
file_size: 19286002
relation: main_file
success: 1
- access_level: open_access
checksum: 6b40f99fddfa26f0a9713000769741b7
content_type: application/octet-stream
creator: jdanzl
date_created: 2023-08-03T13:51:48Z
date_updated: 2023-08-03T13:51:48Z
file_id: '13809'
file_name: Suppl08_e_7-10_roi.roi
file_size: 174
relation: main_file
success: 1
- access_level: open_access
checksum: 82f7013959df99c27366886de9b38ba2
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:51:49Z
date_updated: 2023-08-03T13:51:49Z
file_id: '13810'
file_name: Suppl08_e_9-10_coCATS.tiff
file_size: 19286002
relation: main_file
success: 1
- access_level: open_access
checksum: 50f0969c6c2c1209ff776bec4861030a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:51:48Z
date_updated: 2023-08-03T13:51:48Z
file_id: '13811'
file_name: Suppl08_e_9.tif
file_size: 617552
relation: main_file
success: 1
- access_level: open_access
checksum: 569f599d7f2be49d4dcf7cb385f428c4
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:51:49Z
date_updated: 2023-08-03T13:51:49Z
file_id: '13812'
file_name: Suppl08_e_10.tif
file_size: 617552
relation: main_file
success: 1
- access_level: open_access
checksum: 50f0969c6c2c1209ff776bec4861030a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:51:49Z
date_updated: 2023-08-03T13:51:49Z
file_id: '13813'
file_name: Suppl08_g_1.tif
file_size: 617552
relation: main_file
success: 1
- access_level: open_access
checksum: 62797fc860176197142a47e831eaf1f6
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:51:49Z
date_updated: 2023-08-03T13:51:49Z
file_id: '13814'
file_name: Suppl08_g_2.tif
file_size: 617552
relation: main_file
success: 1
- access_level: open_access
checksum: b90965d20133fea73c99050c32eb7695
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:51:49Z
date_updated: 2023-08-03T13:51:49Z
file_id: '13815'
file_name: Suppl08_g_3.tif
file_size: 617552
relation: main_file
success: 1
- access_level: open_access
checksum: a96dc9f38c6a1d3c959771a649a42748
content_type: application/octet-stream
creator: jdanzl
date_created: 2023-08-03T13:51:49Z
date_updated: 2023-08-03T13:51:49Z
file_id: '13816'
file_name: Suppl08_i_add_roi.roi
file_size: 160
relation: main_file
success: 1
- access_level: open_access
checksum: f2135207ebbc4bdc014763ba852a5cf0
content_type: application/octet-stream
creator: jdanzl
date_created: 2023-08-03T13:51:50Z
date_updated: 2023-08-03T13:51:50Z
file_id: '13817'
file_name: Suppl08_i_shape_roi.roi
file_size: 160
relation: main_file
success: 1
- access_level: open_access
checksum: f6f31fe2d87cf7208387f98ce1e37b04
content_type: application/octet-stream
creator: jdanzl
date_created: 2023-08-03T13:51:50Z
date_updated: 2023-08-03T13:51:50Z
file_id: '13818'
file_name: Suppl08_i_split_roi.roi
file_size: 158
relation: main_file
success: 1
- access_level: open_access
checksum: 71590db0ad391e0c5baf03024981b1d9
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:51:51Z
date_updated: 2023-08-03T13:51:51Z
file_id: '13819'
file_name: Suppl08_i_1.tiff
file_size: 19286002
relation: main_file
success: 1
- access_level: open_access
checksum: 9ce944049c1b88fadbf43609385f9e2f
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:51:51Z
date_updated: 2023-08-03T13:51:51Z
file_id: '13820'
file_name: Suppl08_i_2.tiff
file_size: 19286002
relation: main_file
success: 1
- access_level: open_access
checksum: 06265515f06688843a415362f7b2c485
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:51:52Z
date_updated: 2023-08-03T13:51:52Z
file_id: '13821'
file_name: Suppl08_i_3.tiff
file_size: 19286002
relation: main_file
success: 1
- access_level: open_access
checksum: 16c628c160fb81e40df0ad1eb134e92b
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:51:52Z
date_updated: 2023-08-03T13:51:52Z
file_id: '13822'
file_name: Suppl08_i_4.tif
file_size: 19284766
relation: main_file
success: 1
- access_level: open_access
checksum: b06e62c3a9834a40002d7aad3eaacc3f
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:51:53Z
date_updated: 2023-08-03T13:51:53Z
file_id: '13823'
file_name: Suppl08_i_5.tif
file_size: 9649818
relation: main_file
success: 1
- access_level: open_access
checksum: 41ef98c7f01755b43cf6e77fff287732
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:51:53Z
date_updated: 2023-08-03T13:51:53Z
file_id: '13824'
file_name: Suppl08_i_6.tif
file_size: 19284766
relation: main_file
success: 1
- access_level: open_access
checksum: feae08c38b1d9c540b697977cd7f2666
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:53:29Z
date_updated: 2023-08-03T13:53:29Z
file_id: '13825'
file_name: Suppl08_c_2_coCATS.tif
file_size: 19287340
relation: main_file
success: 1
- access_level: open_access
checksum: c01509a1c657fbbbfdbbd484c298b6e3
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:58:08Z
date_updated: 2023-08-03T13:58:08Z
file_id: '13826'
file_name: Supplfig09_b_1_n2v.tiff
file_size: 179788642
relation: main_file
success: 1
- access_level: open_access
checksum: 64a7f270274ff3480b18d690087016e2
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:58:20Z
date_updated: 2023-08-03T13:58:20Z
file_id: '13827'
file_name: Supplfig09_b_2_n2v.tif
file_size: 742420240
relation: main_file
success: 1
- access_level: open_access
checksum: b34a6beaaf13698f421df961e009efeb
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:58:20Z
date_updated: 2023-08-03T13:58:20Z
file_id: '13828'
file_name: Supplfig09_b_3_n2v.tif
file_size: 478900363
relation: main_file
success: 1
- access_level: open_access
checksum: ec04501f234b5e4fd91f9eac0e585395
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:58:25Z
date_updated: 2023-08-03T13:58:25Z
file_id: '13829'
file_name: Supplfig09_b_4_n2v.tif
file_size: 191873159
relation: main_file
success: 1
- access_level: open_access
checksum: 3a1b313298da35c97550abf0d245b4a7
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:59:27Z
date_updated: 2023-08-03T13:59:27Z
file_id: '13830'
file_name: Supplfig11_i.tif
file_size: 55286
relation: main_file
success: 1
- access_level: open_access
checksum: 9cf00c0252f0a2efe1994c83c224389a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:59:27Z
date_updated: 2023-08-03T13:59:27Z
file_id: '13831'
file_name: Supplfig11_ii.tif
file_size: 55286
relation: main_file
success: 1
- access_level: open_access
checksum: 9a6e83acdf6d70bb9a6e76fa7d41a372
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:59:27Z
date_updated: 2023-08-03T13:59:27Z
file_id: '13832'
file_name: Supplfig11_iii.tif
file_size: 55257
relation: main_file
success: 1
- access_level: open_access
checksum: 709fdd13b7e090846a4d8edd2425f675
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T13:59:32Z
date_updated: 2023-08-03T13:59:32Z
file_id: '13833'
file_name: Supplfig11_n2v+prediction.tif
file_size: 309457551
relation: main_file
success: 1
- access_level: open_access
checksum: e60ea3e6bb1bf380460d0d8ccdbd1f07
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:14:32Z
date_updated: 2023-08-03T14:14:32Z
file_id: '13834'
file_name: Supplfig12_a_01_n2v.tif
file_size: 119323629
relation: main_file
success: 1
- access_level: open_access
checksum: 2a4e1771e2f06383894361ba2a8155b4
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:14:29Z
date_updated: 2023-08-03T14:14:29Z
file_id: '13835'
file_name: Supplfig12_a_01.tif
file_size: 59680727
relation: main_file
success: 1
- access_level: open_access
checksum: 993c434069b9d1bcb09bfe70628f5bbe
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:14:34Z
date_updated: 2023-08-03T14:14:34Z
file_id: '13836'
file_name: Supplfig12_a_02_n2v.tif
file_size: 106696749
relation: main_file
success: 1
- access_level: open_access
checksum: e3b6410d68e42004ef3857967707aeb3
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:14:34Z
date_updated: 2023-08-03T14:14:34Z
file_id: '13837'
file_name: Supplfig12_a_02.tif
file_size: 53367287
relation: main_file
success: 1
- access_level: open_access
checksum: 0156e99806fe1539d5f48ea16c78e8c8
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:14:41Z
date_updated: 2023-08-03T14:14:41Z
file_id: '13838'
file_name: Supplfig12_a_03_n2v.tif
file_size: 149937979
relation: main_file
success: 1
- access_level: open_access
checksum: 6cc1533a221526a072a0b09fb44bcf50
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:14:39Z
date_updated: 2023-08-03T14:14:39Z
file_id: '13839'
file_name: Supplfig12_a_03.tif
file_size: 74987927
relation: main_file
success: 1
- access_level: open_access
checksum: f134834bd0d5e8c22e5a35aadf592007
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:14:53Z
date_updated: 2023-08-03T14:14:53Z
file_id: '13840'
file_name: Supplfig12_a_04_n2v.tif
file_size: 305581862
relation: main_file
success: 1
- access_level: open_access
checksum: 212ec76c02552fa1eef91e7bca9f07f7
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:14:50Z
date_updated: 2023-08-03T14:14:50Z
file_id: '13841'
file_name: Supplfig12_a_04.tif
file_size: 152821840
relation: main_file
success: 1
- access_level: open_access
checksum: 7d99807d5b4313b04b4b5e0fbc08ba78
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:14:52Z
date_updated: 2023-08-03T14:14:52Z
file_id: '13842'
file_name: Supplfig12_a_05_n2v.tif
file_size: 48082029
relation: main_file
success: 1
- access_level: open_access
checksum: a6d4024a8c6fa073a532cc0c78323df7
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:14:53Z
date_updated: 2023-08-03T14:14:53Z
file_id: '13843'
file_name: Supplfig12_a_05.tif
file_size: 24059983
relation: main_file
success: 1
- access_level: open_access
checksum: 1c42ad5423636478fe57495a3b506746
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:15:09Z
date_updated: 2023-08-03T14:15:09Z
file_id: '13844'
file_name: Supplfig12_a_06_n2v.tif
file_size: 305581860
relation: main_file
success: 1
- access_level: open_access
checksum: 821f1d31c6dbe68eb2513fb7520b5272
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:15:04Z
date_updated: 2023-08-03T14:15:04Z
file_id: '13845'
file_name: Supplfig12_a_06.tif
file_size: 152821840
relation: main_file
success: 1
- access_level: open_access
checksum: 86a4dc1e4948c4bc9c71e6d55b4fd104
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:15:08Z
date_updated: 2023-08-03T14:15:08Z
file_id: '13846'
file_name: Supplfig12_a_07_n2v.tif
file_size: 66855793
relation: main_file
success: 1
- access_level: open_access
checksum: fd5d45b1135800124fec635c4667dafe
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:15:09Z
date_updated: 2023-08-03T14:15:09Z
file_id: '13847'
file_name: Supplfig12_a_07.tif
file_size: 33446807
relation: main_file
success: 1
- access_level: open_access
checksum: 5683760cc119a09f0b7de8fea98cda9c
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:15:19Z
date_updated: 2023-08-03T14:15:19Z
file_id: '13848'
file_name: Supplfig12_a_08_n2v.tif
file_size: 231476260
relation: main_file
success: 1
- access_level: open_access
checksum: 0c336d554bcd8524f11bd25c2188b56f
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:15:15Z
date_updated: 2023-08-03T14:15:15Z
file_id: '13849'
file_name: Supplfig12_a_08.tif
file_size: 115769040
relation: main_file
success: 1
- access_level: open_access
checksum: fcaea6c95c363bd864a524fca8ed8e9b
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:15:31Z
date_updated: 2023-08-03T14:15:31Z
file_id: '13850'
file_name: Supplfig12_a_09_n2v.tif
file_size: 305581869
relation: main_file
success: 1
- access_level: open_access
checksum: f401f1875f072c345d106a1e419f9d13
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:15:28Z
date_updated: 2023-08-03T14:15:28Z
file_id: '13851'
file_name: Supplfig12_a_09.tif
file_size: 152821840
relation: main_file
success: 1
- access_level: open_access
checksum: 7ad7d5b1ac81da1ec3d5003d00ee870a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:15:42Z
date_updated: 2023-08-03T14:15:42Z
file_id: '13852'
file_name: Supplfig12_a_10_n2v.tif
file_size: 266386255
relation: main_file
success: 1
- access_level: open_access
checksum: 77b93d082debd5248348c0a1e886eab9
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:15:40Z
date_updated: 2023-08-03T14:15:40Z
file_id: '13853'
file_name: Supplfig12_a_10.tif
file_size: 133239040
relation: main_file
success: 1
- access_level: open_access
checksum: 5ece1d503f05133d07d5b867f666d7a8
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:15:49Z
date_updated: 2023-08-03T14:15:49Z
file_id: '13854'
file_name: Supplfig12_a_11_n2v.tif
file_size: 191866269
relation: main_file
success: 1
- access_level: open_access
checksum: a72ee7ab433097752ff00a63421d4b61
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:15:48Z
date_updated: 2023-08-03T14:15:48Z
file_id: '13855'
file_name: Supplfig12_a_11.tif
file_size: 95955040
relation: main_file
success: 1
- access_level: open_access
checksum: c9c6e4692e0e7f305a43058b201b50d3
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:16:49Z
date_updated: 2023-08-03T14:16:49Z
file_id: '13856'
file_name: Supplfig13_i.tif
file_size: 55281
relation: main_file
success: 1
- access_level: open_access
checksum: 345d11dbd70123d9d2723b547cbfc4fc
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:16:49Z
date_updated: 2023-08-03T14:16:49Z
file_id: '13857'
file_name: Supplfig13_ii.tif
file_size: 55193
relation: main_file
success: 1
- access_level: open_access
checksum: b4c74c08077cfd3e11bfd6ea9ee1c5b2
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:16:49Z
date_updated: 2023-08-03T14:16:49Z
file_id: '13858'
file_name: Supplfig13_n2v+predict.tif
file_size: 539037
relation: main_file
success: 1
- access_level: open_access
checksum: 15f5b44257d075f9e45811304dfd5f85
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:18:10Z
date_updated: 2023-08-03T14:18:10Z
file_id: '13859'
file_name: Supplfig14_a_n2v.tif
file_size: 854898609
relation: main_file
success: 1
- access_level: open_access
checksum: a9ca03e3e5696988d06fc6ab1c103440
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T14:19:58Z
date_updated: 2023-08-03T14:19:58Z
file_id: '13860'
file_name: Supplfig15_a_1.png
file_size: 7514502
relation: main_file
success: 1
- access_level: open_access
checksum: 4be3451e203a0fc5d9eb14279ff68a7a
content_type: image/png
creator: jdanzl
date_created: 2023-08-03T14:19:58Z
date_updated: 2023-08-03T14:19:58Z
file_id: '13861'
file_name: Supplfig15_a_2.png
file_size: 1784041
relation: main_file
success: 1
- access_level: open_access
checksum: 43068c31eecddb798dbf2f51bda9b6c9
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:19:58Z
date_updated: 2023-08-03T14:19:58Z
file_id: '13862'
file_name: Supplfig15_b_1.tif
file_size: 6030878
relation: main_file
success: 1
- access_level: open_access
checksum: ded4c0797f1a9152dc3128f1bb8c32a5
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:19:58Z
date_updated: 2023-08-03T14:19:58Z
file_id: '13863'
file_name: Supplfig15_b_2.tif
file_size: 723293
relation: main_file
success: 1
- access_level: open_access
checksum: 705394732d94bd8463479aee83c2d36e
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:19:58Z
date_updated: 2023-08-03T14:19:58Z
file_id: '13864'
file_name: Supplfig15_c_i.tif
file_size: 723409
relation: main_file
success: 1
- access_level: open_access
checksum: e08efe220bec6a93d583cab17754dd19
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:19:58Z
date_updated: 2023-08-03T14:19:58Z
file_id: '13865'
file_name: Supplfig15_c_ii.tif
file_size: 723409
relation: main_file
success: 1
- access_level: open_access
checksum: 0e08e5d965815240b0a8195e0f899aab
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:19:59Z
date_updated: 2023-08-03T14:19:59Z
file_id: '13866'
file_name: Supplfig15_c.tif
file_size: 31572914
relation: main_file
success: 1
- access_level: open_access
checksum: 594696a77d4a9be512046be5e2d602f0
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:19:59Z
date_updated: 2023-08-03T14:19:59Z
file_id: '13867'
file_name: Supplfig15_d_1.tif
file_size: 6030752
relation: main_file
success: 1
- access_level: open_access
checksum: ffe6733eb77858008c4ca07b5f8c432b
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:19:59Z
date_updated: 2023-08-03T14:19:59Z
file_id: '13868'
file_name: Supplfig15_d_2.tif
file_size: 8005495
relation: main_file
success: 1
- access_level: open_access
checksum: 663bf013cf1e5019e1e7ddd2d12fa492
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:20:56Z
date_updated: 2023-08-03T14:20:56Z
file_id: '13869'
file_name: Supplfig16_a.tif
file_size: 11117709
relation: main_file
success: 1
- access_level: open_access
checksum: 80aee233dc2a1f26ed8cc323270cf3a9
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:20:56Z
date_updated: 2023-08-03T14:20:56Z
file_id: '13870'
file_name: Supplfig16_b.tif
file_size: 113709
relation: main_file
success: 1
- access_level: open_access
checksum: 3b408e9345df2f16a942ad4ea61739b7
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:20:56Z
date_updated: 2023-08-03T14:20:56Z
file_id: '13871'
file_name: Supplfig16_c.tif
file_size: 113709
relation: main_file
success: 1
- access_level: open_access
checksum: fbf688ef1f907149f92e9e936d02df72
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:25:47Z
date_updated: 2023-08-03T14:25:47Z
file_id: '13872'
file_name: Supplfig17_a_1.tif
file_size: 6003131
relation: main_file
success: 1
- access_level: open_access
checksum: 9a56470f251f14b43903036fab338f76
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:25:47Z
date_updated: 2023-08-03T14:25:47Z
file_id: '13873'
file_name: Supplfig17_a_2.tif
file_size: 8004085
relation: main_file
success: 1
- access_level: open_access
checksum: 4578c020ba5d0a6ffe2a8a1c22e52c75
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:25:47Z
date_updated: 2023-08-03T14:25:47Z
file_id: '13874'
file_name: Supplfig17_a_3.tif
file_size: 6003123
relation: main_file
success: 1
- access_level: open_access
checksum: a681d32f515da3bff8e803946f1d8b50
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:25:47Z
date_updated: 2023-08-03T14:25:47Z
file_id: '13875'
file_name: Supplfig17_a_4.tif
file_size: 6003131
relation: main_file
success: 1
- access_level: open_access
checksum: b89d366c39e25d17dc5d8ceee9145c8b
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:25:47Z
date_updated: 2023-08-03T14:25:47Z
file_id: '13876'
file_name: Supplfig17_a_5.tif
file_size: 6003131
relation: main_file
success: 1
- access_level: open_access
checksum: 0b9d60f0aba79525a97c122d972f0b22
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:25:47Z
date_updated: 2023-08-03T14:25:47Z
file_id: '13877'
file_name: Supplfig17_a_6.tif
file_size: 6003079
relation: main_file
success: 1
- access_level: open_access
checksum: 169aad3cea521cc2a0ce4c3e41473e0c
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:25:47Z
date_updated: 2023-08-03T14:25:47Z
file_id: '13878'
file_name: Supplfig17_a_7.tif
file_size: 6003115
relation: main_file
success: 1
- access_level: open_access
checksum: 39a44d77d9047bfa10da8d9c9bd7b391
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:25:47Z
date_updated: 2023-08-03T14:25:47Z
file_id: '13879'
file_name: Supplfig17_a_8.tif
file_size: 4002129
relation: main_file
success: 1
- access_level: open_access
checksum: f4ecb2bd44f2cea3d71587670344ac40
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:25:48Z
date_updated: 2023-08-03T14:25:48Z
file_id: '13880'
file_name: Supplfig17_b.tif
file_size: 8214335
relation: main_file
success: 1
- access_level: open_access
checksum: 44a20d2621680c90227416bf32b5a566
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:27:06Z
date_updated: 2023-08-03T14:27:06Z
file_id: '13881'
file_name: Supplfig18_a.tif
file_size: 7471479
relation: main_file
success: 1
- access_level: open_access
checksum: 5a5f7b616785f9213064e8c77f9d38c4
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:27:06Z
date_updated: 2023-08-03T14:27:06Z
file_id: '13882'
file_name: Supplfig18_b.tif
file_size: 290625
relation: main_file
success: 1
- access_level: open_access
checksum: 8f621722a636e808a03d85f9597ad1a2
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:27:07Z
date_updated: 2023-08-03T14:27:07Z
file_id: '13883'
file_name: Supplfig18_c.tif
file_size: 411767
relation: main_file
success: 1
- access_level: open_access
checksum: 3c8356f9170fe345e2ef725cd7d1b167
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:27:07Z
date_updated: 2023-08-03T14:27:07Z
file_id: '13884'
file_name: Supplfig18_d.tif
file_size: 11117685
relation: main_file
success: 1
- access_level: open_access
checksum: 5c8443e97724b54f359f7355483b890e
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:27:10Z
date_updated: 2023-08-03T14:27:10Z
file_id: '13885'
file_name: Supplfig18_e_n2v.tif
file_size: 259509773
relation: main_file
success: 1
- access_level: open_access
checksum: 413e3105c1bcd2ac2c569fa2b06253f2
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:29:25Z
date_updated: 2023-08-03T14:29:25Z
file_id: '13886'
file_name: Supplfig19_n2v.tif
file_size: 1430939360
relation: main_file
success: 1
- access_level: open_access
checksum: a864dc5b54f469c723e86286d538bb52
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:29:16Z
date_updated: 2023-08-03T14:29:16Z
file_id: '13887'
file_name: Supplfig19.tif
file_size: 715524468
relation: main_file
success: 1
- access_level: open_access
checksum: 8a78ce49446d207c0bf31196b788fa93
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:32:54Z
date_updated: 2023-08-03T14:32:54Z
file_id: '13888'
file_name: Supplfig20_a_left.tif
file_size: 8004743
relation: main_file
success: 1
- access_level: open_access
checksum: 48fa3abfd16a00c7a3757d6452a482ec
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:32:54Z
date_updated: 2023-08-03T14:32:54Z
file_id: '13889'
file_name: Supplfig20_a_right.tif
file_size: 5561081
relation: main_file
success: 1
- access_level: open_access
checksum: 85d82cd94dd3202f04b5987bc4466573
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:32:54Z
date_updated: 2023-08-03T14:32:54Z
file_id: '13890'
file_name: Supplfig20_b_1.tif
file_size: 8456797
relation: main_file
success: 1
- access_level: open_access
checksum: e16f04d5d4d40f6ec33456d0cf02e1eb
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:32:54Z
date_updated: 2023-08-03T14:32:54Z
file_id: '13891'
file_name: Supplfig20_b_2.tif
file_size: 8456795
relation: main_file
success: 1
- access_level: open_access
checksum: ff190edb269c28b25d27bffaeb7d25cf
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:32:54Z
date_updated: 2023-08-03T14:32:54Z
file_id: '13892'
file_name: Supplfig20_b_3.tif
file_size: 8456796
relation: main_file
success: 1
- access_level: open_access
checksum: deb10b81bae62d9e20e681411fb75fcc
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:32:54Z
date_updated: 2023-08-03T14:32:54Z
file_id: '13893'
file_name: Supplfig20_b_4.tif
file_size: 8538887
relation: main_file
success: 1
- access_level: open_access
checksum: 5c64bb94e76030d7a9d8624bac4b0f57
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:32:54Z
date_updated: 2023-08-03T14:32:54Z
file_id: '13894'
file_name: Supplfig20_b_5.tif
file_size: 8538851
relation: main_file
success: 1
- access_level: open_access
checksum: d6c20232c4bd36e213b4c456c5009644
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:32:54Z
date_updated: 2023-08-03T14:32:54Z
file_id: '13895'
file_name: Supplfig20_c_1.tif
file_size: 11077737
relation: main_file
success: 1
- access_level: open_access
checksum: 49c3396b8960af3f6fd8f6b078971d51
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:32:54Z
date_updated: 2023-08-03T14:32:54Z
file_id: '13896'
file_name: Supplfig20_c_2.tif
file_size: 11077737
relation: main_file
success: 1
- access_level: open_access
checksum: 73471528f691fb337839b84c31014da4
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:32:55Z
date_updated: 2023-08-03T14:32:55Z
file_id: '13897'
file_name: Supplfig20_c_3.tif
file_size: 11077737
relation: main_file
success: 1
- access_level: open_access
checksum: 47a0fd1a36d7e7907ce18007bdaaa145
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:32:55Z
date_updated: 2023-08-03T14:32:55Z
file_id: '13898'
file_name: Supplfig20_c_4.tif
file_size: 11077737
relation: main_file
success: 1
- access_level: open_access
checksum: 0c74b07f13fb08a91e61669368f38c9a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:32:55Z
date_updated: 2023-08-03T14:32:55Z
file_id: '13899'
file_name: Supplfig20_c_5.tif
file_size: 11077737
relation: main_file
success: 1
- access_level: open_access
checksum: fe3b76446ec8bd0d5cdba94a128a84b8
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:32:55Z
date_updated: 2023-08-03T14:32:55Z
file_id: '13900'
file_name: Supplfig20_d_1.tif
file_size: 4002133
relation: main_file
success: 1
- access_level: open_access
checksum: 0570bab9a471af3d0d1d8bf9a3cb17e7
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:32:55Z
date_updated: 2023-08-03T14:32:55Z
file_id: '13901'
file_name: Supplfig20_d_2.tif
file_size: 4002133
relation: main_file
success: 1
- access_level: open_access
checksum: 59f330990b5c95f98a768761a009940c
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:32:55Z
date_updated: 2023-08-03T14:32:55Z
file_id: '13902'
file_name: Supplfig20_d_3.tif
file_size: 4002199
relation: main_file
success: 1
- access_level: open_access
checksum: f0da06f2a278dff4bbd1aeace11700bf
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:32:55Z
date_updated: 2023-08-03T14:32:55Z
file_id: '13903'
file_name: Supplfig20_d_4.tif
file_size: 4002133
relation: main_file
success: 1
- access_level: open_access
checksum: e61d49dcc0952af3623cc26a78cc6495
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:32:55Z
date_updated: 2023-08-03T14:32:55Z
file_id: '13904'
file_name: Supplfig20_d_5.tif
file_size: 4002133
relation: main_file
success: 1
- access_level: open_access
checksum: bcf8294487abaf1a75033f388fba06ec
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:42:01Z
date_updated: 2023-08-03T14:42:01Z
file_id: '13905'
file_name: Supplfig22_b_1.tif
file_size: 12627576
relation: main_file
success: 1
- access_level: open_access
checksum: 14097feb4a73e7bb1e1694cef0115cab
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:42:01Z
date_updated: 2023-08-03T14:42:01Z
file_id: '13906'
file_name: Supplfig22_b_2.tif
file_size: 8543366
relation: main_file
success: 1
- access_level: open_access
checksum: 7e74b8cff2ada56d47d135a6a9e71e76
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:42:04Z
date_updated: 2023-08-03T14:42:04Z
file_id: '13907'
file_name: Supplfig22_b_3.tif
file_size: 8394010
relation: main_file
success: 1
- access_level: open_access
checksum: f6e6e0f0e3854fedd201ab8ff08a7651
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T15:35:39Z
date_updated: 2023-08-03T15:35:39Z
file_id: '13908'
file_name: Supplfig22_b_4.tif
file_size: 8682992
relation: main_file
success: 1
- access_level: open_access
checksum: 6ddeb84249768b46b9c2072bf85bdc8b
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:42:04Z
date_updated: 2023-08-03T14:42:04Z
file_id: '13909'
file_name: Supplfig22_b_5.tif
file_size: 8503466
relation: main_file
success: 1
- access_level: open_access
checksum: 40648b8a4ef375b8352f11a6772ea1a6
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:42:04Z
date_updated: 2023-08-03T14:42:04Z
file_id: '13910'
file_name: Supplfig22_b_6.tif
file_size: 8543342
relation: main_file
success: 1
- access_level: open_access
checksum: 78f601de4d88f6ccb97861eaf4a995f8
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:42:06Z
date_updated: 2023-08-03T14:42:06Z
file_id: '13911'
file_name: Supplfig22_b_7.tif
file_size: 33588990
relation: main_file
success: 1
- access_level: open_access
checksum: 2873c488b7c8ad4d111726025086e9ea
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:42:41Z
date_updated: 2023-08-03T14:42:41Z
file_id: '13912'
file_name: Supplfig22_c.tif
file_size: 1453171518
relation: main_file
success: 1
- access_level: open_access
checksum: c1d0e5e61ac0d0ddf08704769f80ac78
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:47:43Z
date_updated: 2023-08-03T14:47:43Z
file_id: '13913'
file_name: Supplfig23_a.tif
file_size: 1325919782
relation: main_file
success: 1
- access_level: open_access
checksum: f97632984b6a73e06cc0c8c947cdcc71
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:47:25Z
date_updated: 2023-08-03T14:47:25Z
file_id: '13914'
file_name: Supplfig23_b.tif
file_size: 31366288
relation: main_file
success: 1
- access_level: open_access
checksum: 16f4c17835a79d7c06e2a97b1603baf1
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:47:26Z
date_updated: 2023-08-03T14:47:26Z
file_id: '13915'
file_name: Supplfig23_c.tif
file_size: 31366288
relation: main_file
success: 1
- access_level: open_access
checksum: 00f35ca77165642ca9b3bc3608bd04c1
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:47:27Z
date_updated: 2023-08-03T14:47:27Z
file_id: '13916'
file_name: Supplfig23_d.tif
file_size: 31366428
relation: main_file
success: 1
- access_level: open_access
checksum: 1aff850f47ae8c8f05ca48ff9faa38b1
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:47:27Z
date_updated: 2023-08-03T14:47:27Z
file_id: '13917'
file_name: Supplfig23_e.tif
file_size: 31500832
relation: main_file
success: 1
- access_level: open_access
checksum: f644de697d142c83461ed5ad1d72a043
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:48:38Z
date_updated: 2023-08-03T14:48:38Z
file_id: '13918'
file_name: Supplfig24_a_i.tif
file_size: 44179078
relation: main_file
success: 1
- access_level: open_access
checksum: eccb84925a6699b396d4b07efcbc7349
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:48:38Z
date_updated: 2023-08-03T14:48:38Z
file_id: '13919'
file_name: Supplfig24_a_ii.tif
file_size: 44125924
relation: main_file
success: 1
- access_level: open_access
checksum: afe7253e70ec79144e59b28c3c66703a
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:48:54Z
date_updated: 2023-08-03T14:48:54Z
file_id: '13920'
file_name: Supplfig24_a.tif
file_size: 1061835435
relation: main_file
success: 1
- access_level: open_access
checksum: 94a03b022aa84ea6e8515450a481ad82
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:48:39Z
date_updated: 2023-08-03T14:48:39Z
file_id: '13921'
file_name: Supplfig24_b_iii.tif
file_size: 3977824
relation: main_file
success: 1
- access_level: open_access
checksum: 166832c173fcda88ec6e35decdf0d197
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:48:39Z
date_updated: 2023-08-03T14:48:39Z
file_id: '13922'
file_name: Supplfig24_b_iv.tif
file_size: 3977825
relation: main_file
success: 1
- access_level: open_access
checksum: 53175073b83ab569241ae64ef7f180b3
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:51:27Z
date_updated: 2023-08-03T14:51:27Z
file_id: '13923'
file_name: Supplfig25_a_1.tif
file_size: 37641738
relation: main_file
success: 1
- access_level: open_access
checksum: 70e6a1780daeae6a5bef2c27c996c442
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:51:26Z
date_updated: 2023-08-03T14:51:26Z
file_id: '13924'
file_name: Supplfig25_a_2.tif
file_size: 4932897
relation: main_file
success: 1
- access_level: open_access
checksum: 1748266d7b8a2ae1f6292b9c265c2068
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:51:31Z
date_updated: 2023-08-03T14:51:31Z
file_id: '13925'
file_name: Supplfig25_b_1.tif
file_size: 357433570
relation: main_file
success: 1
- access_level: open_access
checksum: 8490f44160a482ea06d858eb3bc25a11
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:51:27Z
date_updated: 2023-08-03T14:51:27Z
file_id: '13926'
file_name: Supplfig25_b_2.tif
file_size: 27734506
relation: main_file
success: 1
- access_level: open_access
checksum: e5e9c550cc934a7725dc209c79951847
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:53:43Z
date_updated: 2023-08-03T14:53:43Z
file_id: '13927'
file_name: Suppfig26_h.tif
file_size: 35082394
relation: main_file
success: 1
- access_level: open_access
checksum: 39ae411d1e950e78bdc12937fb36f661
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:53:42Z
date_updated: 2023-08-03T14:53:42Z
file_id: '13928'
file_name: Supplfig26_a_1.tif
file_size: 16676477
relation: main_file
success: 1
- access_level: open_access
checksum: 450f5d0e8091639e27eb7a7991524e88
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:53:42Z
date_updated: 2023-08-03T14:53:42Z
file_id: '13929'
file_name: Supplfig26_a_2.tif
file_size: 170493
relation: main_file
success: 1
- access_level: open_access
checksum: 8e5a71801fa1fcb7f679d1af23344662
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:53:43Z
date_updated: 2023-08-03T14:53:43Z
file_id: '13930'
file_name: Supplfig26_b_1.tif
file_size: 11117737
relation: main_file
success: 1
- access_level: open_access
checksum: 519fb0326a0f0774b3edc1dafffcdd08
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:53:43Z
date_updated: 2023-08-03T14:53:43Z
file_id: '13931'
file_name: Supplfig26_b_2.tif
file_size: 113753
relation: main_file
success: 1
- access_level: open_access
checksum: d051c7d717eeb6f2ca59d8727ea62200
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:53:52Z
date_updated: 2023-08-03T14:53:52Z
file_id: '13932'
file_name: Supplfig26_c.tif
file_size: 495452386
relation: main_file
success: 1
- access_level: open_access
checksum: 5437b53e708572e2e9ad1432c260a04f
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:53:46Z
date_updated: 2023-08-03T14:53:46Z
file_id: '13933'
file_name: Supplfig26_d.tif
file_size: 132167170
relation: main_file
success: 1
- access_level: open_access
checksum: 0baab38829a8587ded5e39f85068124b
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:53:46Z
date_updated: 2023-08-03T14:53:46Z
file_id: '13934'
file_name: Supplfig26_e.tif
file_size: 11431214
relation: main_file
success: 1
- access_level: open_access
checksum: afa57f254c9dd987d90d5e43db3fb9e6
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:53:48Z
date_updated: 2023-08-03T14:53:48Z
file_id: '13935'
file_name: Supplfig26_f.tif
file_size: 64005702
relation: main_file
success: 1
- access_level: open_access
checksum: c35ba67ab92c410cb4370f91f3dea819
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:53:52Z
date_updated: 2023-08-03T14:53:52Z
file_id: '13936'
file_name: Supplfig26_g.tif
file_size: 246421054
relation: main_file
success: 1
- access_level: open_access
checksum: 3fd6186d73cbce1be79cf14a94a7f3bd
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:56:43Z
date_updated: 2023-08-03T14:56:43Z
file_id: '13937'
file_name: Supplfig27_a.tif
file_size: 166614932
relation: main_file
success: 1
- access_level: open_access
checksum: d1fc4c88e8e258e4e536a26e196d09b0
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:56:42Z
date_updated: 2023-08-03T14:56:42Z
file_id: '13938'
file_name: Supplfig27_b.tif
file_size: 143583446
relation: main_file
success: 1
- access_level: open_access
checksum: 8d6985530e86f38548e89e6380d73771
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:56:47Z
date_updated: 2023-08-03T14:56:47Z
file_id: '13939'
file_name: Supplfig27_c.tif
file_size: 172528188
relation: main_file
success: 1
- access_level: open_access
checksum: d0b1309d2120892ede84e04fdf0dbfbf
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:56:46Z
date_updated: 2023-08-03T14:56:46Z
file_id: '13940'
file_name: Supplfig27_d.tif
file_size: 113699256
relation: main_file
success: 1
- access_level: open_access
checksum: 4789b5254d0415ce314fc9e39cc52121
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:56:47Z
date_updated: 2023-08-03T14:56:47Z
file_id: '13941'
file_name: Supplfig27_e.tif
file_size: 88664548
relation: main_file
success: 1
- access_level: open_access
checksum: 7ff2d06534f062b0f17dab181f6c1f4e
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:56:47Z
date_updated: 2023-08-03T14:56:47Z
file_id: '13942'
file_name: Supplfig27_f.tif
file_size: 2298356
relation: main_file
success: 1
- access_level: open_access
checksum: 46de66d457b0def90f753c11927536b7
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:56:47Z
date_updated: 2023-08-03T14:56:47Z
file_id: '13943'
file_name: Supplfig27_g_1.tif
file_size: 499618
relation: main_file
success: 1
- access_level: open_access
checksum: 9ef5fba064a73a79dbc74b5e166d08ec
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:56:50Z
date_updated: 2023-08-03T14:56:50Z
file_id: '13944'
file_name: Supplfig27_g_2.tif
file_size: 202079026
relation: main_file
success: 1
- access_level: open_access
checksum: bf956c2b58d3da2baa869db5206d8d49
content_type: image/tiff
creator: jdanzl
date_created: 2023-08-03T14:56:48Z
date_updated: 2023-08-03T14:56:48Z
file_id: '13945'
file_name: Supplfig27_g_3.tif
file_size: 14639269
relation: main_file
success: 1
- access_level: open_access
checksum: 327dca11f42d5375d7f47f4cbd5b36c0
content_type: application/octet-stream
creator: jdanzl
date_created: 2023-08-03T15:21:01Z
date_updated: 2023-08-03T15:21:01Z
file_id: '13946'
file_name: model.p
file_size: 279467712
relation: main_file
success: 1
file_date_updated: 2023-08-04T13:19:47Z
has_accepted_license: '1'
license: https://creativecommons.org/licenses/by-nc-sa/4.0/
month: '08'
oa: 1
oa_version: Published Version
project:
- _id: 265CB4D0-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03600
name: Optical control of synaptic function via adhesion molecules
- _id: 26AA4EF2-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: W1232-B24
name: Molecular Drug Targets
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: The Wittgenstein Prize
- _id: 23889792-32DE-11EA-91FC-C7463DDC885E
name: High content imaging to decode human immune cell interactions in health and
allergic disease
- _id: 25444568-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '715508'
name: Probing the Reversibility of Autism Spectrum Disorders by Employing in vivo
and in vitro Models
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
- _id: fc2be41b-9c52-11eb-aca3-faa90aa144e9
call_identifier: H2020
grant_number: '101026635'
name: Synaptic computations of the hippocampal CA3 circuitry
publisher: Institute of Science and Technology Austria
related_material:
link:
- description: 'Original data for Fig. 5d, Fig. 5d (N2V) and Fig. 5f-i, provided
via an external link due to the large size (>10GB) of the datasets. '
relation: research_data
url: https://pub.ista.ac.at/group_danzl/data/CATS/
record:
- id: '14257'
relation: used_in_publication
status: public
status: public
title: Research data for the publication "Imaging brain tissue architecture across
millimeter to nanometer scales"
tmp:
image: /images/cc_by_nc_sa.png
legal_code_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC
BY-NC-SA 4.0)
short: CC BY-NC-SA (4.0)
type: research_data
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2023'
...
---
_id: '14257'
abstract:
- lang: eng
text: Mapping the complex and dense arrangement of cells and their connectivity
in brain tissue demands nanoscale spatial resolution imaging. Super-resolution
optical microscopy excels at visualizing specific molecules and individual cells
but fails to provide tissue context. Here we developed Comprehensive Analysis
of Tissues across Scales (CATS), a technology to densely map brain tissue architecture
from millimeter regional to nanometer synaptic scales in diverse chemically fixed
brain preparations, including rodent and human. CATS uses fixation-compatible
extracellular labeling and optical imaging, including stimulated emission depletion
or expansion microscopy, to comprehensively delineate cellular structures. It
enables three-dimensional reconstruction of single synapses and mapping of synaptic
connectivity by identification and analysis of putative synaptic cleft regions.
Applying CATS to the mouse hippocampal mossy fiber circuitry, we reconstructed
and quantified the synaptic input and output structure of identified neurons.
We furthermore demonstrate applicability to clinically derived human tissue samples,
including formalin-fixed paraffin-embedded routine diagnostic specimens, for visualizing
the cellular architecture of brain tissue in health and disease.
acknowledged_ssus:
- _id: ScienComp
- _id: Bio
- _id: PreCl
- _id: LifeSc
- _id: M-Shop
- _id: E-Lib
acknowledgement: 'We thank J. Vorlaufer, N. Agudelo-Dueñas, W. Jahr and A. Wartak
for microscope maintenance and troubleshooting; C. Kreuzinger, A. Freeman and I.
Erber for technical assistance; and M. Tomschik for support with obtaining human
samples. We gratefully acknowledge E. Miguel for setting up webKnossos and M. Šuplata
for computational support and hardware control. We are grateful to R. Shigemoto
and B. Bickel for generous support and M. Sixt and S. Boyd (Stanford University)
for discussions and critical reading of the paper. PSD95-HaloTag mice were kindly
provided by S. Grant (University of Edinburgh). We acknowledge expert support by
Institute of Science and Technology Austria’s scientific computing, imaging and
optics, preclinical and lab support facilities and by the Miba machine shop and
library. We gratefully acknowledge funding by the following sources: Austrian Science
Fund (FWF) grant I3600-B27 (J.G.D.); Austrian Science Fund (FWF) grant DK W1232
(J.G.D. and J.M.M.); Austrian Science Fund (FWF) grant Z 312-B27, Wittgenstein award
(P.J.); Austrian Science Fund (FWF) projects I4685-B, I6565-B (SYNABS) and DOC 33-B27
(R.H.); Gesellschaft für Forschungsförderung NÖ (NFB) grant LSC18-022 (J.G.D.);
European Union’s Horizon 2020 research and innovation programme, European Research
Council (ERC) grant 715508 – REVERSEAUTISM (G.N.); European Union’s Horizon 2020
research and innovation programme, European Research Council (ERC) grant 692692
– GIANTSYN (P.J.); Marie Skłodowska-Curie Actions Fellowship GA no. 665385 under
the EU Horizon 2020 program (J.M.M. and J.L.); and Marie Skłodowska-Curie Actions
Individual Fellowship no. 101026635 under the EU Horizon 2020 program (J.F.W.).'
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Julia M
full_name: Michalska, Julia M
id: 443DB6DE-F248-11E8-B48F-1D18A9856A87
last_name: Michalska
orcid: 0000-0003-3862-1235
- first_name: Julia
full_name: Lyudchik, Julia
id: 46E28B80-F248-11E8-B48F-1D18A9856A87
last_name: Lyudchik
- first_name: Philipp
full_name: Velicky, Philipp
id: 39BDC62C-F248-11E8-B48F-1D18A9856A87
last_name: Velicky
orcid: 0000-0002-2340-7431
- first_name: Hana
full_name: Korinkova, Hana
id: ee3cb6ca-ec98-11ea-ae11-ff703e2254ed
last_name: Korinkova
- first_name: Jake
full_name: Watson, Jake
id: 63836096-4690-11EA-BD4E-32803DDC885E
last_name: Watson
orcid: 0000-0002-8698-3823
- first_name: Alban
full_name: Cenameri, Alban
id: 9ac8f577-2357-11eb-997a-e566c5550886
last_name: Cenameri
- first_name: Christoph M
full_name: Sommer, Christoph M
id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
last_name: Sommer
orcid: 0000-0003-1216-9105
- first_name: Nicole
full_name: Amberg, Nicole
id: 4CD6AAC6-F248-11E8-B48F-1D18A9856A87
last_name: Amberg
orcid: 0000-0002-3183-8207
- first_name: Alessandro
full_name: Venturino, Alessandro
id: 41CB84B2-F248-11E8-B48F-1D18A9856A87
last_name: Venturino
orcid: 0000-0003-2356-9403
- first_name: Karl
full_name: Roessler, Karl
last_name: Roessler
- first_name: Thomas
full_name: Czech, Thomas
last_name: Czech
- first_name: Romana
full_name: Höftberger, Romana
last_name: Höftberger
- first_name: Sandra
full_name: Siegert, Sandra
id: 36ACD32E-F248-11E8-B48F-1D18A9856A87
last_name: Siegert
orcid: 0000-0001-8635-0877
- first_name: Gaia
full_name: Novarino, Gaia
id: 3E57A680-F248-11E8-B48F-1D18A9856A87
last_name: Novarino
orcid: 0000-0002-7673-7178
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: Johann G
full_name: Danzl, Johann G
id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87
last_name: Danzl
orcid: 0000-0001-8559-3973
citation:
ama: Michalska JM, Lyudchik J, Velicky P, et al. Imaging brain tissue architecture
across millimeter to nanometer scales. Nature Biotechnology. 2023. doi:10.1038/s41587-023-01911-8
apa: Michalska, J. M., Lyudchik, J., Velicky, P., Korinkova, H., Watson, J., Cenameri,
A., … Danzl, J. G. (2023). Imaging brain tissue architecture across millimeter
to nanometer scales. Nature Biotechnology. Springer Nature. https://doi.org/10.1038/s41587-023-01911-8
chicago: Michalska, Julia M, Julia Lyudchik, Philipp Velicky, Hana Korinkova, Jake
Watson, Alban Cenameri, Christoph M Sommer, et al. “Imaging Brain Tissue Architecture
across Millimeter to Nanometer Scales.” Nature Biotechnology. Springer
Nature, 2023. https://doi.org/10.1038/s41587-023-01911-8.
ieee: J. M. Michalska et al., “Imaging brain tissue architecture across millimeter
to nanometer scales,” Nature Biotechnology. Springer Nature, 2023.
ista: Michalska JM, Lyudchik J, Velicky P, Korinkova H, Watson J, Cenameri A, Sommer
CM, Amberg N, Venturino A, Roessler K, Czech T, Höftberger R, Siegert S, Novarino
G, Jonas PM, Danzl JG. 2023. Imaging brain tissue architecture across millimeter
to nanometer scales. Nature Biotechnology.
mla: Michalska, Julia M., et al. “Imaging Brain Tissue Architecture across Millimeter
to Nanometer Scales.” Nature Biotechnology, Springer Nature, 2023, doi:10.1038/s41587-023-01911-8.
short: J.M. Michalska, J. Lyudchik, P. Velicky, H. Korinkova, J. Watson, A. Cenameri,
C.M. Sommer, N. Amberg, A. Venturino, K. Roessler, T. Czech, R. Höftberger, S.
Siegert, G. Novarino, P.M. Jonas, J.G. Danzl, Nature Biotechnology (2023).
date_created: 2023-09-03T22:01:15Z
date_published: 2023-08-31T00:00:00Z
date_updated: 2024-02-21T12:18:18Z
day: '31'
department:
- _id: SaSi
- _id: GaNo
- _id: PeJo
- _id: JoDa
- _id: Bio
- _id: RySh
doi: 10.1038/s41587-023-01911-8
ec_funded: 1
external_id:
isi:
- '001065254200001'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1038/s41587-023-01911-8
month: '08'
oa: 1
oa_version: Published Version
project:
- _id: 265CB4D0-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03600
name: Optical control of synaptic function via adhesion molecules
- _id: 2548AE96-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: W1232-B24
name: Molecular Drug Targets
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: The Wittgenstein Prize
- _id: 23889792-32DE-11EA-91FC-C7463DDC885E
name: High content imaging to decode human immune cell interactions in health and
allergic disease
- _id: 25444568-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '715508'
name: Probing the Reversibility of Autism Spectrum Disorders by Employing in vivo
and in vitro Models
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
- _id: fc2be41b-9c52-11eb-aca3-faa90aa144e9
call_identifier: H2020
grant_number: '101026635'
name: Synaptic computations of the hippocampal CA3 circuitry
publication: Nature Biotechnology
publication_identifier:
eissn:
- 1546-1696
issn:
- 1087-0156
publication_status: epub_ahead
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- relation: software
url: https://github.com/danzllab/CATS
record:
- id: '13126'
relation: research_data
status: public
scopus_import: '1'
status: public
title: Imaging brain tissue architecture across millimeter to nanometer scales
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2023'
...
---
_id: '10763'
abstract:
- lang: eng
text: "AMPA-type glutamate receptors (AMPARs) mediate rapid signal transmission
at excitatory\r\nsynapses in the brain. Glutamate binding to the receptor’s ligand-binding
domains (LBDs)\r\nleads to ion channel activation and desensitization. Gating
kinetics shape synaptic transmission\r\nand are strongly modulated by transmembrane
AMPAR regulatory proteins (TARPs)\r\nthrough currently incompletely resolved mechanisms.
Here, electron cryo-microscopy\r\nstructures of the GluA1/2 TARP-γ8 complex, in
both open and desensitized states\r\n(at 3.5 Å), reveal state-selective engagement
of the LBDs by the large TARP-γ8 loop (‘β1’),\r\nelucidating how this TARP stabilizes
specific gating states. We further show how TARPs alter\r\nchannel rectification,
by interacting with the pore helix of the selectivity filter. Lastly, we\r\nreveal
that the Q/R-editing site couples the channel constriction at the filter entrance
to the\r\ngate, and forms the major cation binding site in the conduction path.
Our results provide a\r\nmechanistic framework of how TARPs modulate AMPAR gating
and conductance."
acknowledgement: "We thank Ondrej Cais for critical reading of the manuscript. We
are grateful to LMB\r\nscientific computing and the EM facility for support, Paul
Emsley for help with model\r\nbuilding and Takanori Nakane for helpful comments
with Relion 3.1. This work was\r\nsupported by grants from the Medical Research
Council (MC_U105174197) and BBSRC\r\n(BB/N002113/1) to I.H.G, and grants from the
MCIN/AEI/ 10.13039/501100011033 and\r\n“ESF Investing in your future” to B.H (PID2019-106284GA-I00
and RYC2018-025720-I)."
article_number: '734'
article_processing_charge: No
article_type: original
author:
- first_name: Beatriz
full_name: Herguedas, Beatriz
last_name: Herguedas
- first_name: Bianka K.
full_name: Kohegyi, Bianka K.
last_name: Kohegyi
- first_name: Jan Niklas
full_name: Dohrke, Jan Niklas
last_name: Dohrke
- first_name: Jake
full_name: Watson, Jake
id: 63836096-4690-11EA-BD4E-32803DDC885E
last_name: Watson
orcid: 0000-0002-8698-3823
- first_name: Danyang
full_name: Zhang, Danyang
last_name: Zhang
- first_name: Hinze
full_name: Ho, Hinze
last_name: Ho
- first_name: Saher A.
full_name: Shaikh, Saher A.
last_name: Shaikh
- first_name: Remigijus
full_name: Lape, Remigijus
last_name: Lape
- first_name: James M.
full_name: Krieger, James M.
last_name: Krieger
- first_name: Ingo H.
full_name: Greger, Ingo H.
last_name: Greger
citation:
ama: Herguedas B, Kohegyi BK, Dohrke JN, et al. Mechanisms underlying TARP modulation
of the GluA1/2-γ8 AMPA receptor. Nature Communications. 2022;13. doi:10.1038/s41467-022-28404-7
apa: Herguedas, B., Kohegyi, B. K., Dohrke, J. N., Watson, J., Zhang, D., Ho, H.,
… Greger, I. H. (2022). Mechanisms underlying TARP modulation of the GluA1/2-γ8
AMPA receptor. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-022-28404-7
chicago: Herguedas, Beatriz, Bianka K. Kohegyi, Jan Niklas Dohrke, Jake Watson,
Danyang Zhang, Hinze Ho, Saher A. Shaikh, Remigijus Lape, James M. Krieger, and
Ingo H. Greger. “Mechanisms Underlying TARP Modulation of the GluA1/2-Γ8 AMPA
Receptor.” Nature Communications. Springer Nature, 2022. https://doi.org/10.1038/s41467-022-28404-7.
ieee: B. Herguedas et al., “Mechanisms underlying TARP modulation of the
GluA1/2-γ8 AMPA receptor,” Nature Communications, vol. 13. Springer Nature,
2022.
ista: Herguedas B, Kohegyi BK, Dohrke JN, Watson J, Zhang D, Ho H, Shaikh SA, Lape
R, Krieger JM, Greger IH. 2022. Mechanisms underlying TARP modulation of the GluA1/2-γ8
AMPA receptor. Nature Communications. 13, 734.
mla: Herguedas, Beatriz, et al. “Mechanisms Underlying TARP Modulation of the GluA1/2-Γ8
AMPA Receptor.” Nature Communications, vol. 13, 734, Springer Nature, 2022,
doi:10.1038/s41467-022-28404-7.
short: B. Herguedas, B.K. Kohegyi, J.N. Dohrke, J. Watson, D. Zhang, H. Ho, S.A.
Shaikh, R. Lape, J.M. Krieger, I.H. Greger, Nature Communications 13 (2022).
date_created: 2022-02-20T23:01:30Z
date_published: 2022-02-08T00:00:00Z
date_updated: 2023-08-02T14:25:33Z
day: '08'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1038/s41467-022-28404-7
external_id:
isi:
- '000757297200008'
pmid:
- '35136046'
file:
- access_level: open_access
checksum: d86ee8eabe8b794730729ffbb1a8832e
content_type: application/pdf
creator: dernst
date_created: 2022-02-21T07:59:32Z
date_updated: 2022-02-21T07:59:32Z
file_id: '10778'
file_name: 2022_NatureCommunications_Herguedas.pdf
file_size: 2625540
relation: main_file
success: 1
file_date_updated: 2022-02-21T07:59:32Z
has_accepted_license: '1'
intvolume: ' 13'
isi: 1
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
publication: Nature Communications
publication_identifier:
eissn:
- '20411723'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Mechanisms underlying TARP modulation of the GluA1/2-γ8 AMPA receptor
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 13
year: '2022'
...
---
_id: '11951'
abstract:
- lang: eng
text: The mammalian hippocampal formation (HF) plays a key role in several higher
brain functions, such as spatial coding, learning and memory. Its simple circuit
architecture is often viewed as a trisynaptic loop, processing input originating
from the superficial layers of the entorhinal cortex (EC) and sending it back
to its deeper layers. Here, we show that excitatory neurons in layer 6b of the
mouse EC project to all sub-regions comprising the HF and receive input from the
CA1, thalamus and claustrum. Furthermore, their output is characterized by unique
slow-decaying excitatory postsynaptic currents capable of driving plateau-like
potentials in their postsynaptic targets. Optogenetic inhibition of the EC-6b
pathway affects spatial coding in CA1 pyramidal neurons, while cell ablation impairs
not only acquisition of new spatial memories, but also degradation of previously
acquired ones. Our results provide evidence of a functional role for cortical
layer 6b neurons in the adult brain.
acknowledged_ssus:
- _id: Bio
- _id: SSU
acknowledgement: We thank F. Marr and A. Schlögl for technical assistance, E. Kralli-Beller
for manuscript editing, as well as C. Sommer and the Imaging and Optics Facility
of the Institute of Science and Technology Austria (ISTA) for image analysis scripts
and microscopy support. We extend our gratitude to J. Wallenschus and D. Rangel
Guerrero for technical assistance acquiring single-unit data and I. Gridchyn for
help with single-unit clustering. Finally, we also thank B. Suter for discussions,
A. Saunders, M. Jösch, and H. Monyer for critically reading earlier versions of
the manuscript, C. Petersen for sharing clearing protocols, and the Scientific Service
Units of ISTA for efficient support. This project was funded by the European Research
Council (ERC) under the European Union’s Horizon 2020 research and innovation programme
(ERC advanced grant No 692692 to P.J.) and the Fond zur Förderung der Wissenschaftlichen
Forschung (Z 312-B27, Wittgenstein award for P.J. and I3600-B27 for J.G.D. and P.V.).
article_number: '4826'
article_processing_charge: No
article_type: original
author:
- first_name: Yoav
full_name: Ben Simon, Yoav
id: 43DF3136-F248-11E8-B48F-1D18A9856A87
last_name: Ben Simon
- first_name: Karola
full_name: Käfer, Karola
id: 2DAA49AA-F248-11E8-B48F-1D18A9856A87
last_name: Käfer
- first_name: Philipp
full_name: Velicky, Philipp
id: 39BDC62C-F248-11E8-B48F-1D18A9856A87
last_name: Velicky
orcid: 0000-0002-2340-7431
- first_name: Jozsef L
full_name: Csicsvari, Jozsef L
id: 3FA14672-F248-11E8-B48F-1D18A9856A87
last_name: Csicsvari
orcid: 0000-0002-5193-4036
- first_name: Johann G
full_name: Danzl, Johann G
id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87
last_name: Danzl
orcid: 0000-0001-8559-3973
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Ben Simon Y, Käfer K, Velicky P, Csicsvari JL, Danzl JG, Jonas PM. A direct
excitatory projection from entorhinal layer 6b neurons to the hippocampus contributes
to spatial coding and memory. Nature Communications. 2022;13. doi:10.1038/s41467-022-32559-8
apa: Ben Simon, Y., Käfer, K., Velicky, P., Csicsvari, J. L., Danzl, J. G., &
Jonas, P. M. (2022). A direct excitatory projection from entorhinal layer 6b neurons
to the hippocampus contributes to spatial coding and memory. Nature Communications.
Springer Nature. https://doi.org/10.1038/s41467-022-32559-8
chicago: Ben Simon, Yoav, Karola Käfer, Philipp Velicky, Jozsef L Csicsvari, Johann
G Danzl, and Peter M Jonas. “A Direct Excitatory Projection from Entorhinal Layer
6b Neurons to the Hippocampus Contributes to Spatial Coding and Memory.” Nature
Communications. Springer Nature, 2022. https://doi.org/10.1038/s41467-022-32559-8.
ieee: Y. Ben Simon, K. Käfer, P. Velicky, J. L. Csicsvari, J. G. Danzl, and P. M.
Jonas, “A direct excitatory projection from entorhinal layer 6b neurons to the
hippocampus contributes to spatial coding and memory,” Nature Communications,
vol. 13. Springer Nature, 2022.
ista: Ben Simon Y, Käfer K, Velicky P, Csicsvari JL, Danzl JG, Jonas PM. 2022. A
direct excitatory projection from entorhinal layer 6b neurons to the hippocampus
contributes to spatial coding and memory. Nature Communications. 13, 4826.
mla: Ben Simon, Yoav, et al. “A Direct Excitatory Projection from Entorhinal Layer
6b Neurons to the Hippocampus Contributes to Spatial Coding and Memory.” Nature
Communications, vol. 13, 4826, Springer Nature, 2022, doi:10.1038/s41467-022-32559-8.
short: Y. Ben Simon, K. Käfer, P. Velicky, J.L. Csicsvari, J.G. Danzl, P.M. Jonas,
Nature Communications 13 (2022).
date_created: 2022-08-24T08:25:50Z
date_published: 2022-08-16T00:00:00Z
date_updated: 2023-08-03T13:01:19Z
day: '16'
ddc:
- '570'
department:
- _id: JoCs
- _id: PeJo
- _id: JoDa
doi: 10.1038/s41467-022-32559-8
ec_funded: 1
external_id:
isi:
- '000841396400008'
file:
- access_level: open_access
checksum: 405936d9e4d33625d80c093c9713a91f
content_type: application/pdf
creator: dernst
date_created: 2022-08-26T11:51:40Z
date_updated: 2022-08-26T11:51:40Z
file_id: '11990'
file_name: 2022_NatureCommunications_BenSimon.pdf
file_size: 5910357
relation: main_file
success: 1
file_date_updated: 2022-08-26T11:51:40Z
has_accepted_license: '1'
intvolume: ' 13'
isi: 1
keyword:
- General Physics and Astronomy
- General Biochemistry
- Genetics and Molecular Biology
- General Chemistry
- Multidisciplinary
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: 265CB4D0-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03600
name: Optical control of synaptic function via adhesion molecules
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: The Wittgenstein Prize
publication: Nature Communications
publication_identifier:
issn:
- 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
status: public
title: A direct excitatory projection from entorhinal layer 6b neurons to the hippocampus
contributes to spatial coding and memory
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 13
year: '2022'
...
---
_id: '12288'
abstract:
- lang: eng
text: To understand the function of neuronal circuits, it is crucial to disentangle
the connectivity patterns within the network. However, most tools currently used
to explore connectivity have low throughput, low selectivity, or limited accessibility.
Here, we report the development of an improved packaging system for the production
of the highly neurotropic RVdGenvA-CVS-N2c rabies viral vectors, yielding titers
orders of magnitude higher with no background contamination, at a fraction of
the production time, while preserving the efficiency of transsynaptic labeling.
Along with the production pipeline, we developed suites of ‘starter’ AAV and bicistronic
RVdG-CVS-N2c vectors, enabling retrograde labeling from a wide range of neuronal
populations, tailored for diverse experimental requirements. We demonstrate the
power and flexibility of the new system by uncovering hidden local and distal
inhibitory connections in the mouse hippocampal formation and by imaging the functional
properties of a cortical microcircuit across weeks. Our novel production pipeline
provides a convenient approach to generate new rabies vectors, while our toolkit
flexibly and efficiently expands the current capacity to label, manipulate and
image the neuronal activity of interconnected neuronal circuits in vitro and in
vivo.
acknowledged_ssus:
- _id: Bio
- _id: PreCl
acknowledgement: We thank F Marr for technical assistance, A Murray for RVdG-CVS-N2c
viruses and Neuro2A packaging cell-lines and J Watson for reading the manuscript.
This research was supported by the Scientific Service Units (SSU) of IST-Austria
through resources provided by the Imaging and Optics Facility (IOF) and the Preclinical
Facility (PCF). This project was funded by the European Research Council (ERC) under
the European Union’s Horizon 2020 research and innovation programme (ERC advanced
grant No 692692, PJ, ERC starting grant No 756502, MJ), the Fond zur Förderung der
Wissenschaftlichen Forschung (Z 312-B27, Wittgenstein award, PJ), the Human Frontier
Science Program (LT000256/2018-L, AS) and EMBO (ALTF 1098-2017, AS).
article_number: '79848'
article_processing_charge: No
article_type: original
author:
- first_name: Anton L
full_name: Sumser, Anton L
id: 3320A096-F248-11E8-B48F-1D18A9856A87
last_name: Sumser
orcid: 0000-0002-4792-1881
- first_name: Maximilian A
full_name: Jösch, Maximilian A
id: 2BD278E6-F248-11E8-B48F-1D18A9856A87
last_name: Jösch
orcid: 0000-0002-3937-1330
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: Yoav
full_name: Ben Simon, Yoav
id: 43DF3136-F248-11E8-B48F-1D18A9856A87
last_name: Ben Simon
citation:
ama: Sumser AL, Jösch MA, Jonas PM, Ben Simon Y. Fast, high-throughput production
of improved rabies viral vectors for specific, efficient and versatile transsynaptic
retrograde labeling. eLife. 2022;11. doi:10.7554/elife.79848
apa: Sumser, A. L., Jösch, M. A., Jonas, P. M., & Ben Simon, Y. (2022). Fast,
high-throughput production of improved rabies viral vectors for specific, efficient
and versatile transsynaptic retrograde labeling. ELife. eLife Sciences
Publications. https://doi.org/10.7554/elife.79848
chicago: Sumser, Anton L, Maximilian A Jösch, Peter M Jonas, and Yoav Ben Simon.
“Fast, High-Throughput Production of Improved Rabies Viral Vectors for Specific,
Efficient and Versatile Transsynaptic Retrograde Labeling.” ELife. eLife
Sciences Publications, 2022. https://doi.org/10.7554/elife.79848.
ieee: A. L. Sumser, M. A. Jösch, P. M. Jonas, and Y. Ben Simon, “Fast, high-throughput
production of improved rabies viral vectors for specific, efficient and versatile
transsynaptic retrograde labeling,” eLife, vol. 11. eLife Sciences Publications,
2022.
ista: Sumser AL, Jösch MA, Jonas PM, Ben Simon Y. 2022. Fast, high-throughput production
of improved rabies viral vectors for specific, efficient and versatile transsynaptic
retrograde labeling. eLife. 11, 79848.
mla: Sumser, Anton L., et al. “Fast, High-Throughput Production of Improved Rabies
Viral Vectors for Specific, Efficient and Versatile Transsynaptic Retrograde Labeling.”
ELife, vol. 11, 79848, eLife Sciences Publications, 2022, doi:10.7554/elife.79848.
short: A.L. Sumser, M.A. Jösch, P.M. Jonas, Y. Ben Simon, ELife 11 (2022).
date_created: 2023-01-16T10:04:15Z
date_published: 2022-09-15T00:00:00Z
date_updated: 2023-08-04T10:29:48Z
day: '15'
ddc:
- '570'
department:
- _id: MaJö
- _id: PeJo
doi: 10.7554/elife.79848
ec_funded: 1
external_id:
isi:
- '000892204300001'
pmid:
- '36040301'
file:
- access_level: open_access
checksum: 5a2a65e3e7225090c3d8199f3bbd7b7b
content_type: application/pdf
creator: dernst
date_created: 2023-01-30T11:50:53Z
date_updated: 2023-01-30T11:50:53Z
file_id: '12463'
file_name: 2022_eLife_Sumser.pdf
file_size: 8506811
relation: main_file
success: 1
file_date_updated: 2023-01-30T11:50:53Z
has_accepted_license: '1'
intvolume: ' 11'
isi: 1
keyword:
- General Immunology and Microbiology
- General Biochemistry
- Genetics and Molecular Biology
- General Medicine
- General Neuroscience
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: 2634E9D2-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '756502'
name: Circuits of Visual Attention
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: The Wittgenstein Prize
- _id: 266D407A-B435-11E9-9278-68D0E5697425
grant_number: LT000256
name: Neuronal networks of salience and spatial detection in the murine superior
colliculus
- _id: 264FEA02-B435-11E9-9278-68D0E5697425
grant_number: ALTF 1098-2017
name: Connecting sensory with motor processing in the superior colliculus
publication: eLife
publication_identifier:
eissn:
- 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: Fast, high-throughput production of improved rabies viral vectors for specific,
efficient and versatile transsynaptic retrograde labeling
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 11
year: '2022'
...
---
_id: '11196'
abstract:
- lang: eng
text: "One of the fundamental questions in Neuroscience is how the structure of
synapses and their physiological properties are related. While synaptic transmission
remains a dynamic process, electron microscopy provides images with comparably
low temporal resolution (Studer et al., 2014). The current work overcomes this
challenge and describes an improved “Flash and Freeze” technique (Watanabe et
al., 2013a; Watanabe et al., 2013b) to study synaptic transmission at the hippocampal
mossy fiber-CA3 pyramidal neuron synapses, using mouse acute brain slices and
organotypic slices culture. The improved method allowed for selective stimulation
of presynaptic mossy fiber boutons and the observation of synaptic vesicle pool
dynamics at the active zones. Our results uncovered several intriguing morphological
features of mossy fiber boutons. First, the docked vesicle pool was largely depleted
(more than 70%) after stimulation, implying that the docked synaptic vesicles
pool and readily releasable pool are vastly overlapping in mossy fiber boutons.
Second, the synaptic vesicles are skewed towards larger diameters, displaying
a wide range of sizes. An increase in the mean diameter of synaptic vesicles,
after single and repetitive stimulation, suggests that smaller vesicles have a
higher release probability. Third, we observed putative endocytotic structures
after moderate light stimulation, matching the timing of previously described
ultrafast endocytosis (Watanabe et al., 2013a; Delvendahl et al., 2016). \r\n\tIn
addition, synaptic transmission depends on a sophisticated system of protein machinery
and calcium channels (Südhof, 2013b), which amplifies the challenge in studying
synaptic communication as these interactions can be potentially modified during
synaptic plasticity. And although recent study elucidated the potential correlation
between physiological and morphological properties of synapses during synaptic
plasticity (Vandael et al., 2020), the molecular underpinning of it remains unknown.
Thus, the presented work tries to overcome this challenge and aims to pinpoint
changes in the molecular architecture at hippocampal mossy fiber bouton synapses
during short- and long-term potentiation (STP and LTP), we combined chemical potentiation,
with the application of a cyclic adenosine monophosphate agonist (i.e. forskolin)
and freeze-fracture replica immunolabelling. This method allowed the localization
of membrane-bound proteins with nanometer precision within the active zone, in
particular, P/Q-type calcium channels and synaptic vesicle priming proteins Munc13-1/2.
First, we found that the number of clusters of Munc13-1 in the mossy fiber bouton
active zone increased significantly during STP, but decreased to lower than the
control value during LTP. Secondly, although the distance between the calcium
channels and Munc13-1s did not change after induction of STP, it shortened during
the LTP phase. Additionally, forskolin did not affect Munc13-2 distribution during
STP and LTP. These results indicate the existence of two distinct mechanisms that
govern STP and LTP at mossy fiber bouton synapses: an increase in the readily
realizable pool in the case of STP and a potential increase in release probability
during LTP. “Flash and freeze” and functional electron microscopy, are versatile
methods that can be successfully applied to intact brain circuits to study synaptic
transmission even at the molecular level.\r\n"
acknowledged_ssus:
- _id: EM-Fac
- _id: PreCl
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Olena
full_name: Kim, Olena
id: 3F8ABDDA-F248-11E8-B48F-1D18A9856A87
last_name: Kim
citation:
ama: Kim O. Nanoarchitecture of hippocampal mossy fiber-CA3 pyramidal neuron synapses.
2022. doi:10.15479/at:ista:11196
apa: Kim, O. (2022). Nanoarchitecture of hippocampal mossy fiber-CA3 pyramidal
neuron synapses. Institute of Science and Technology Austria. https://doi.org/10.15479/at:ista:11196
chicago: Kim, Olena. “Nanoarchitecture of Hippocampal Mossy Fiber-CA3 Pyramidal
Neuron Synapses.” Institute of Science and Technology Austria, 2022. https://doi.org/10.15479/at:ista:11196.
ieee: O. Kim, “Nanoarchitecture of hippocampal mossy fiber-CA3 pyramidal neuron
synapses,” Institute of Science and Technology Austria, 2022.
ista: Kim O. 2022. Nanoarchitecture of hippocampal mossy fiber-CA3 pyramidal neuron
synapses. Institute of Science and Technology Austria.
mla: Kim, Olena. Nanoarchitecture of Hippocampal Mossy Fiber-CA3 Pyramidal Neuron
Synapses. Institute of Science and Technology Austria, 2022, doi:10.15479/at:ista:11196.
short: O. Kim, Nanoarchitecture of Hippocampal Mossy Fiber-CA3 Pyramidal Neuron
Synapses, Institute of Science and Technology Austria, 2022.
date_created: 2022-04-20T09:47:12Z
date_published: 2022-04-20T00:00:00Z
date_updated: 2023-08-18T06:31:52Z
day: '20'
ddc:
- '570'
degree_awarded: PhD
department:
- _id: PeJo
- _id: GradSch
doi: 10.15479/at:ista:11196
ec_funded: 1
file:
- access_level: open_access
checksum: 1616a8bf6f13a57c892dac873dcd0936
content_type: application/pdf
creator: okim
date_created: 2022-04-20T14:21:56Z
date_updated: 2023-04-20T22:30:03Z
embargo: 2023-04-19
file_id: '11220'
file_name: Olena_KIM_thesis_final.pdf
file_size: 21273537
relation: main_file
- access_level: closed
checksum: 1acb433f98dc42abb0b4b0cbb0c4b918
content_type: application/x-zip-compressed
creator: okim
date_created: 2022-04-20T14:22:56Z
date_updated: 2023-04-20T22:30:03Z
embargo_to: open_access
file_id: '11221'
file_name: KIM_thesis_final.zip
file_size: 59248569
relation: source_file
file_date_updated: 2023-04-20T22:30:03Z
has_accepted_license: '1'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
page: '132'
project:
- _id: 25BAF7B2-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '708497'
name: Presynaptic calcium channels distribution and impact on coupling at the hippocampal
mossy fiber synapse
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: 25C3DBB6-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: W01205
name: Zellkommunikation in Gesundheit und Krankheit
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: The Wittgenstein Prize
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '11222'
relation: part_of_dissertation
status: public
- id: '7473'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
title: Nanoarchitecture of hippocampal mossy fiber-CA3 pyramidal neuron synapses
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: dissertation
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
year: '2022'
...
---
_id: '11943'
abstract:
- lang: eng
text: Complex wiring between neurons underlies the information-processing network
enabling all brain functions, including cognition and memory. For understanding
how the network is structured, processes information, and changes over time, comprehensive
visualization of the architecture of living brain tissue with its cellular and
molecular components would open up major opportunities. However, electron microscopy
(EM) provides nanometre-scale resolution required for full in-silico
reconstruction1–5, yet is limited to fixed specimens and
static representations. Light microscopy allows live observation, with super-resolution
approaches6–12 facilitating nanoscale visualization, but
comprehensive 3D-reconstruction of living brain tissue has been hindered by tissue
photo-burden, photobleaching, insufficient 3D-resolution, and inadequate signal-to-noise
ratio (SNR). Here we demonstrate saturated reconstruction of living brain tissue.
We developed an integrated imaging and analysis technology, adapting stimulated
emission depletion (STED) microscopy6,13 in extracellularly
labelled tissue14 for high SNR and near-isotropic resolution.
Centrally, a two-stage deep-learning approach leveraged previously obtained information
on sample structure to drastically reduce photo-burden and enable automated volumetric
reconstruction down to single synapse level. Live reconstruction provides unbiased
analysis of tissue architecture across time in relation to functional activity
and targeted activation, and contextual understanding of molecular labelling.
This adoptable technology will facilitate novel insights into the dynamic functional
architecture of living brain tissue.
article_processing_charge: No
author:
- first_name: Philipp
full_name: Velicky, Philipp
id: 39BDC62C-F248-11E8-B48F-1D18A9856A87
last_name: Velicky
orcid: 0000-0002-2340-7431
- first_name: Eder
full_name: Miguel Villalba, Eder
id: 3FB91342-F248-11E8-B48F-1D18A9856A87
last_name: Miguel Villalba
orcid: 0000-0001-5665-0430
- first_name: Julia M
full_name: Michalska, Julia M
id: 443DB6DE-F248-11E8-B48F-1D18A9856A87
last_name: Michalska
orcid: 0000-0003-3862-1235
- first_name: Donglai
full_name: Wei, Donglai
last_name: Wei
- first_name: Zudi
full_name: Lin, Zudi
last_name: Lin
- first_name: Jake
full_name: Watson, Jake
id: 63836096-4690-11EA-BD4E-32803DDC885E
last_name: Watson
orcid: 0000-0002-8698-3823
- first_name: Jakob
full_name: Troidl, Jakob
last_name: Troidl
- first_name: Johanna
full_name: Beyer, Johanna
last_name: Beyer
- first_name: Yoav
full_name: Ben Simon, Yoav
id: 43DF3136-F248-11E8-B48F-1D18A9856A87
last_name: Ben Simon
- first_name: Christoph M
full_name: Sommer, Christoph M
id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
last_name: Sommer
orcid: 0000-0003-1216-9105
- first_name: Wiebke
full_name: Jahr, Wiebke
id: 425C1CE8-F248-11E8-B48F-1D18A9856A87
last_name: Jahr
- first_name: Alban
full_name: Cenameri, Alban
id: 9ac8f577-2357-11eb-997a-e566c5550886
last_name: Cenameri
- first_name: Johannes
full_name: Broichhagen, Johannes
last_name: Broichhagen
- first_name: Seth G. N.
full_name: Grant, Seth G. N.
last_name: Grant
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: Gaia
full_name: Novarino, Gaia
id: 3E57A680-F248-11E8-B48F-1D18A9856A87
last_name: Novarino
orcid: 0000-0002-7673-7178
- first_name: Hanspeter
full_name: Pfister, Hanspeter
last_name: Pfister
- first_name: Bernd
full_name: Bickel, Bernd
id: 49876194-F248-11E8-B48F-1D18A9856A87
last_name: Bickel
orcid: 0000-0001-6511-9385
- first_name: Johann G
full_name: Danzl, Johann G
id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87
last_name: Danzl
orcid: 0000-0001-8559-3973
citation:
ama: Velicky P, Miguel Villalba E, Michalska JM, et al. Saturated reconstruction
of living brain tissue. bioRxiv. doi:10.1101/2022.03.16.484431
apa: Velicky, P., Miguel Villalba, E., Michalska, J. M., Wei, D., Lin, Z., Watson,
J., … Danzl, J. G. (n.d.). Saturated reconstruction of living brain tissue. bioRxiv.
Cold Spring Harbor Laboratory. https://doi.org/10.1101/2022.03.16.484431
chicago: Velicky, Philipp, Eder Miguel Villalba, Julia M Michalska, Donglai Wei,
Zudi Lin, Jake Watson, Jakob Troidl, et al. “Saturated Reconstruction of Living
Brain Tissue.” BioRxiv. Cold Spring Harbor Laboratory, n.d. https://doi.org/10.1101/2022.03.16.484431.
ieee: P. Velicky et al., “Saturated reconstruction of living brain tissue,”
bioRxiv. Cold Spring Harbor Laboratory.
ista: Velicky P, Miguel Villalba E, Michalska JM, Wei D, Lin Z, Watson J, Troidl
J, Beyer J, Ben Simon Y, Sommer CM, Jahr W, Cenameri A, Broichhagen J, Grant SGN,
Jonas PM, Novarino G, Pfister H, Bickel B, Danzl JG. Saturated reconstruction
of living brain tissue. bioRxiv, 10.1101/2022.03.16.484431.
mla: Velicky, Philipp, et al. “Saturated Reconstruction of Living Brain Tissue.”
BioRxiv, Cold Spring Harbor Laboratory, doi:10.1101/2022.03.16.484431.
short: P. Velicky, E. Miguel Villalba, J.M. Michalska, D. Wei, Z. Lin, J. Watson,
J. Troidl, J. Beyer, Y. Ben Simon, C.M. Sommer, W. Jahr, A. Cenameri, J. Broichhagen,
S.G.N. Grant, P.M. Jonas, G. Novarino, H. Pfister, B. Bickel, J.G. Danzl, BioRxiv
(n.d.).
date_created: 2022-08-23T11:07:59Z
date_published: 2022-05-09T00:00:00Z
date_updated: 2024-03-27T23:30:20Z
day: '09'
department:
- _id: PeJo
- _id: GaNo
- _id: BeBi
- _id: JoDa
doi: 10.1101/2022.03.16.484431
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2022.03.16.484431
month: '05'
oa: 1
oa_version: Preprint
publication: bioRxiv
publication_status: submitted
publisher: Cold Spring Harbor Laboratory
related_material:
record:
- id: '12470'
relation: dissertation_contains
status: public
status: public
title: Saturated reconstruction of living brain tissue
type: preprint
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2022'
...
---
_id: '11950'
abstract:
- lang: eng
text: Mapping the complex and dense arrangement of cells and their connectivity
in brain tissue demands nanoscale spatial resolution imaging. Super-resolution
optical microscopy excels at visualizing specific molecules and individual cells
but fails to provide tissue context. Here we developed Comprehensive Analysis
of Tissues across Scales (CATS), a technology to densely map brain tissue architecture
from millimeter regional to nanoscopic synaptic scales in diverse chemically fixed
brain preparations, including rodent and human. CATS leverages fixation-compatible
extracellular labeling and advanced optical readout, in particular stimulated-emission
depletion and expansion microscopy, to comprehensively delineate cellular structures.
It enables 3D-reconstructing single synapses and mapping synaptic connectivity
by identification and tailored analysis of putative synaptic cleft regions. Applying
CATS to the hippocampal mossy fiber circuitry, we demonstrate its power to reveal
the system’s molecularly informed ultrastructure across spatial scales and assess
local connectivity by reconstructing and quantifying the synaptic input and output
structure of identified neurons.
article_processing_charge: No
author:
- first_name: Julia M
full_name: Michalska, Julia M
id: 443DB6DE-F248-11E8-B48F-1D18A9856A87
last_name: Michalska
orcid: 0000-0003-3862-1235
- first_name: Julia
full_name: Lyudchik, Julia
id: 46E28B80-F248-11E8-B48F-1D18A9856A87
last_name: Lyudchik
- first_name: Philipp
full_name: Velicky, Philipp
id: 39BDC62C-F248-11E8-B48F-1D18A9856A87
last_name: Velicky
orcid: 0000-0002-2340-7431
- first_name: Hana
full_name: Korinkova, Hana
id: ee3cb6ca-ec98-11ea-ae11-ff703e2254ed
last_name: Korinkova
- first_name: Jake
full_name: Watson, Jake
id: 63836096-4690-11EA-BD4E-32803DDC885E
last_name: Watson
orcid: 0000-0002-8698-3823
- first_name: Alban
full_name: Cenameri, Alban
id: 9ac8f577-2357-11eb-997a-e566c5550886
last_name: Cenameri
- first_name: Christoph M
full_name: Sommer, Christoph M
id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
last_name: Sommer
orcid: 0000-0003-1216-9105
- first_name: Alessandro
full_name: Venturino, Alessandro
id: 41CB84B2-F248-11E8-B48F-1D18A9856A87
last_name: Venturino
orcid: 0000-0003-2356-9403
- first_name: Karl
full_name: Roessler, Karl
last_name: Roessler
- first_name: Thomas
full_name: Czech, Thomas
last_name: Czech
- first_name: Sandra
full_name: Siegert, Sandra
id: 36ACD32E-F248-11E8-B48F-1D18A9856A87
last_name: Siegert
orcid: 0000-0001-8635-0877
- first_name: Gaia
full_name: Novarino, Gaia
id: 3E57A680-F248-11E8-B48F-1D18A9856A87
last_name: Novarino
orcid: 0000-0002-7673-7178
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: Johann G
full_name: Danzl, Johann G
id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87
last_name: Danzl
orcid: 0000-0001-8559-3973
citation:
ama: Michalska JM, Lyudchik J, Velicky P, et al. Uncovering brain tissue architecture
across scales with super-resolution light microscopy. bioRxiv. doi:10.1101/2022.08.17.504272
apa: Michalska, J. M., Lyudchik, J., Velicky, P., Korinkova, H., Watson, J., Cenameri,
A., … Danzl, J. G. (n.d.). Uncovering brain tissue architecture across scales
with super-resolution light microscopy. bioRxiv. Cold Spring Harbor Laboratory.
https://doi.org/10.1101/2022.08.17.504272
chicago: Michalska, Julia M, Julia Lyudchik, Philipp Velicky, Hana Korinkova, Jake
Watson, Alban Cenameri, Christoph M Sommer, et al. “Uncovering Brain Tissue Architecture
across Scales with Super-Resolution Light Microscopy.” BioRxiv. Cold Spring
Harbor Laboratory, n.d. https://doi.org/10.1101/2022.08.17.504272.
ieee: J. M. Michalska et al., “Uncovering brain tissue architecture across
scales with super-resolution light microscopy,” bioRxiv. Cold Spring Harbor
Laboratory.
ista: Michalska JM, Lyudchik J, Velicky P, Korinkova H, Watson J, Cenameri A, Sommer
CM, Venturino A, Roessler K, Czech T, Siegert S, Novarino G, Jonas PM, Danzl JG.
Uncovering brain tissue architecture across scales with super-resolution light
microscopy. bioRxiv, 10.1101/2022.08.17.504272.
mla: Michalska, Julia M., et al. “Uncovering Brain Tissue Architecture across Scales
with Super-Resolution Light Microscopy.” BioRxiv, Cold Spring Harbor Laboratory,
doi:10.1101/2022.08.17.504272.
short: J.M. Michalska, J. Lyudchik, P. Velicky, H. Korinkova, J. Watson, A. Cenameri,
C.M. Sommer, A. Venturino, K. Roessler, T. Czech, S. Siegert, G. Novarino, P.M.
Jonas, J.G. Danzl, BioRxiv (n.d.).
date_created: 2022-08-24T08:24:52Z
date_published: 2022-08-18T00:00:00Z
date_updated: 2024-03-27T23:30:20Z
day: '18'
department:
- _id: SaSi
- _id: GaNo
- _id: PeJo
- _id: JoDa
doi: 10.1101/2022.08.17.504272
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2022.08.17.504272
month: '08'
oa: 1
oa_version: Preprint
publication: bioRxiv
publication_status: submitted
publisher: Cold Spring Harbor Laboratory
related_material:
record:
- id: '12470'
relation: dissertation_contains
status: public
status: public
title: Uncovering brain tissue architecture across scales with super-resolution light
microscopy
type: preprint
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2022'
...
---
_id: '9097'
abstract:
- lang: eng
text: Psoriasis is a chronic inflammatory skin disease clinically characterized
by the appearance of red colored, well-demarcated plaques with thickened skin
and with silvery scales. Recent studies have established the involvement of a
complex signalling network of interactions between cytokines, immune cells and
skin cells called keratinocytes. Keratinocytes form the cells of the outermost
layer of the skin (epidermis). Visible plaques in psoriasis are developed due
to the fast proliferation and unusual differentiation of keratinocyte cells. Despite
that, the exact mechanism of the appearance of these plaques in the cytokine-immune
cell network is not clear. A mathematical model embodying interactions between
key immune cells believed to be involved in psoriasis, keratinocytes and relevant
cytokines has been developed. The complex network formed of these interactions
poses several challenges. Here, we choose to study subnetworks of this complex
network and initially focus on interactions involving TNFα, IL-23/IL-17, and IL-15.
These are chosen based on known evidence of their therapeutic efficacy. In addition,
we explore the role of IL-15 in the pathogenesis of psoriasis and its potential
as a future drug target for a novel treatment option. We perform steady state
analyses for these subnetworks and demonstrate that the interactions between cells,
driven by cytokines could cause the emergence of a psoriasis state (hyper-proliferation
of keratinocytes) when levels of TNFα, IL-23/IL-17 or IL-15 are increased. The
model results explain and support the clinical potentiality of anti-cytokine treatments.
Interestingly, our results suggest different dynamic scenarios underpin the pathogenesis
of psoriasis, depending upon the dominant cytokines of subnetworks. We observed
that the increase in the level of IL-23/IL-17 and IL-15 could lead to psoriasis
via a bistable route, whereas an increase in the level of TNFα would lead to a
monotonic and gradual disease progression. Further, we demonstrate how this insight,
bistability, could be exploited to improve the current therapies and develop novel
treatment strategies for psoriasis.
acknowledgement: RP acknowledges the Department of Science and Technology, India for
the support through the DST-INSPIRE Faculty Award (DST/INSPIRE/04/2015/001939).
This work was supported by the Engineering and Physical Sciences Research Council
(EPSRC), United Kingdom (Grant numbers EP/J018295/1, EP/J018392/1, EP/N014391/1).
The contribution of RP was also supported by the later Grant. This work was generously
supported by the Welcome Trust Institutional Strategic Support Award (204909/Z/16/Z)
too. The contribution of MG was supported by the EPSRC via EP/N014391/1 and a Wellcome
Trust Institutional Strategic Support Award (WT105618MA). The contribution of YA
was generously supported by the Wellcome Trust Institutional Strategic Support Award
(WT105618MA).
article_number: '2204'
article_processing_charge: No
article_type: original
author:
- first_name: Rakesh
full_name: Pandey, Rakesh
last_name: Pandey
- first_name: Yusur
full_name: Al-Nuaimi, Yusur
last_name: Al-Nuaimi
- first_name: Rajiv Kumar
full_name: Mishra, Rajiv Kumar
id: 46CB58F2-F248-11E8-B48F-1D18A9856A87
last_name: Mishra
- first_name: Sarah K.
full_name: Spurgeon, Sarah K.
last_name: Spurgeon
- first_name: Marc
full_name: Goodfellow, Marc
last_name: Goodfellow
citation:
ama: Pandey R, Al-Nuaimi Y, Mishra RK, Spurgeon SK, Goodfellow M. Role of subnetworks
mediated by TNF α, IL-23/IL-17 and IL-15 in a network involved in the pathogenesis
of psoriasis. Scientific Reports. 2021;11. doi:10.1038/s41598-020-80507-7
apa: Pandey, R., Al-Nuaimi, Y., Mishra, R. K., Spurgeon, S. K., & Goodfellow,
M. (2021). Role of subnetworks mediated by TNF α, IL-23/IL-17 and IL-15 in a network
involved in the pathogenesis of psoriasis. Scientific Reports. Springer
Nature. https://doi.org/10.1038/s41598-020-80507-7
chicago: Pandey, Rakesh, Yusur Al-Nuaimi, Rajiv Kumar Mishra, Sarah K. Spurgeon,
and Marc Goodfellow. “Role of Subnetworks Mediated by TNF α, IL-23/IL-17 and IL-15
in a Network Involved in the Pathogenesis of Psoriasis.” Scientific Reports.
Springer Nature, 2021. https://doi.org/10.1038/s41598-020-80507-7.
ieee: R. Pandey, Y. Al-Nuaimi, R. K. Mishra, S. K. Spurgeon, and M. Goodfellow,
“Role of subnetworks mediated by TNF α, IL-23/IL-17 and IL-15 in a network involved
in the pathogenesis of psoriasis,” Scientific Reports, vol. 11. Springer
Nature, 2021.
ista: Pandey R, Al-Nuaimi Y, Mishra RK, Spurgeon SK, Goodfellow M. 2021. Role of
subnetworks mediated by TNF α, IL-23/IL-17 and IL-15 in a network involved in
the pathogenesis of psoriasis. Scientific Reports. 11, 2204.
mla: Pandey, Rakesh, et al. “Role of Subnetworks Mediated by TNF α, IL-23/IL-17
and IL-15 in a Network Involved in the Pathogenesis of Psoriasis.” Scientific
Reports, vol. 11, 2204, Springer Nature, 2021, doi:10.1038/s41598-020-80507-7.
short: R. Pandey, Y. Al-Nuaimi, R.K. Mishra, S.K. Spurgeon, M. Goodfellow, Scientific
Reports 11 (2021).
date_created: 2021-02-07T23:01:12Z
date_published: 2021-01-26T00:00:00Z
date_updated: 2022-08-19T07:22:23Z
day: '26'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1038/s41598-020-80507-7
file:
- access_level: open_access
checksum: e8a68df48750712671f5c47b0228e531
content_type: application/pdf
creator: dernst
date_created: 2021-02-09T07:33:23Z
date_updated: 2021-02-09T07:33:23Z
file_id: '9106'
file_name: 2021_ScientificReports_Pandey.pdf
file_size: 2885056
relation: main_file
success: 1
file_date_updated: 2021-02-09T07:33:23Z
has_accepted_license: '1'
intvolume: ' 11'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
publication: Scientific Reports
publication_identifier:
eissn:
- '20452322'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Role of subnetworks mediated by TNF α, IL-23/IL-17 and IL-15 in a network involved
in the pathogenesis of psoriasis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 11
year: '2021'
...
---
_id: '9329'
abstract:
- lang: eng
text: "Background: To understand information coding in single neurons, it is necessary
to analyze subthreshold synaptic events, action potentials (APs), and their interrelation
in different behavioral states. However, detecting excitatory postsynaptic potentials
(EPSPs) or currents (EPSCs) in behaving animals remains challenging, because of
unfavorable signal-to-noise ratio, high frequency, fluctuating amplitude, and
variable time course of synaptic events.\r\nNew method: We developed a method
for synaptic event detection, termed MOD (Machine-learning Optimal-filtering Detection-procedure),
which combines concepts of supervised machine learning and optimal Wiener filtering.
Experts were asked to manually score short epochs of data. The algorithm was trained
to obtain the optimal filter coefficients of a Wiener filter and the optimal detection
threshold. Scored and unscored data were then processed with the optimal filter,
and events were detected as peaks above threshold.\r\nResults: We challenged MOD
with EPSP traces in vivo in mice during spatial navigation and EPSC traces in
vitro in slices under conditions of enhanced transmitter release. The area under
the curve (AUC) of the receiver operating characteristics (ROC) curve was, on
average, 0.894 for in vivo and 0.969 for in vitro data sets, indicating high detection
accuracy and efficiency.\r\nComparison with existing methods: When benchmarked
using a (1 − AUC)−1 metric, MOD outperformed previous methods (template-fit, deconvolution,
and Bayesian methods) by an average factor of 3.13 for in vivo data sets, but
showed comparable (template-fit, deconvolution) or higher (Bayesian) computational
efficacy.\r\nConclusions: MOD may become an important new tool for large-scale,
real-time analysis of synaptic activity."
acknowledged_ssus:
- _id: SSU
acknowledgement: This project has received funding from the European Research Council
(ERC) under the European Union’s Horizon 2020 research and innovation programme
(grant agreement number 692692 to P.J.) and the Fond zur Förderung der Wissenschaftlichen
Forschung (Z 312-B27, Wittgenstein award to P.J.). We thank Drs. Jozsef Csicsvari,
Christoph Lampert, and Federico Stella for critically reading previous manuscript
versions. We are also grateful to Drs. Josh Merel and Ben Shababo for their help
with applying the Bayesian detection method to our data. We also thank Florian Marr
for technical assistance, Eleftheria Kralli-Beller for manuscript editing, and the
Scientific Service Units of IST Austria for efficient support.
article_number: '109125'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Xiaomin
full_name: Zhang, Xiaomin
id: 423EC9C2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
- first_name: Alois
full_name: Schlögl, Alois
id: 45BF87EE-F248-11E8-B48F-1D18A9856A87
last_name: Schlögl
orcid: 0000-0002-5621-8100
- first_name: David H
full_name: Vandael, David H
id: 3AE48E0A-F248-11E8-B48F-1D18A9856A87
last_name: Vandael
orcid: 0000-0001-7577-1676
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: 'Zhang X, Schlögl A, Vandael DH, Jonas PM. MOD: A novel machine-learning optimal-filtering
method for accurate and efficient detection of subthreshold synaptic events in
vivo. Journal of Neuroscience Methods. 2021;357(6). doi:10.1016/j.jneumeth.2021.109125'
apa: 'Zhang, X., Schlögl, A., Vandael, D. H., & Jonas, P. M. (2021). MOD: A
novel machine-learning optimal-filtering method for accurate and efficient detection
of subthreshold synaptic events in vivo. Journal of Neuroscience Methods.
Elsevier. https://doi.org/10.1016/j.jneumeth.2021.109125'
chicago: 'Zhang, Xiaomin, Alois Schlögl, David H Vandael, and Peter M Jonas. “MOD:
A Novel Machine-Learning Optimal-Filtering Method for Accurate and Efficient Detection
of Subthreshold Synaptic Events in Vivo.” Journal of Neuroscience Methods.
Elsevier, 2021. https://doi.org/10.1016/j.jneumeth.2021.109125.'
ieee: 'X. Zhang, A. Schlögl, D. H. Vandael, and P. M. Jonas, “MOD: A novel machine-learning
optimal-filtering method for accurate and efficient detection of subthreshold
synaptic events in vivo,” Journal of Neuroscience Methods, vol. 357, no.
6. Elsevier, 2021.'
ista: 'Zhang X, Schlögl A, Vandael DH, Jonas PM. 2021. MOD: A novel machine-learning
optimal-filtering method for accurate and efficient detection of subthreshold
synaptic events in vivo. Journal of Neuroscience Methods. 357(6), 109125.'
mla: 'Zhang, Xiaomin, et al. “MOD: A Novel Machine-Learning Optimal-Filtering Method
for Accurate and Efficient Detection of Subthreshold Synaptic Events in Vivo.”
Journal of Neuroscience Methods, vol. 357, no. 6, 109125, Elsevier, 2021,
doi:10.1016/j.jneumeth.2021.109125.'
short: X. Zhang, A. Schlögl, D.H. Vandael, P.M. Jonas, Journal of Neuroscience Methods
357 (2021).
date_created: 2021-04-18T22:01:39Z
date_published: 2021-03-09T00:00:00Z
date_updated: 2023-08-07T14:36:14Z
day: '09'
ddc:
- '570'
department:
- _id: PeJo
- _id: ScienComp
doi: 10.1016/j.jneumeth.2021.109125
ec_funded: 1
external_id:
isi:
- '000661088500005'
file:
- access_level: open_access
checksum: 2a5800d91b96d08b525e17319dcd5e44
content_type: application/pdf
creator: dernst
date_created: 2021-04-19T08:30:22Z
date_updated: 2021-04-19T08:30:22Z
file_id: '9339'
file_name: 2021_JourNeuroscienceMeth_Zhang.pdf
file_size: 6924738
relation: main_file
success: 1
file_date_updated: 2021-04-19T08:30:22Z
has_accepted_license: '1'
intvolume: ' 357'
isi: 1
issue: '6'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: The Wittgenstein Prize
publication: Journal of Neuroscience Methods
publication_identifier:
eissn:
- 1872-678X
issn:
- 0165-0270
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'MOD: A novel machine-learning optimal-filtering method for accurate and efficient
detection of subthreshold synaptic events in vivo'
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 357
year: '2021'
...
---
_id: '9549'
abstract:
- lang: eng
text: 'AMPA receptors (AMPARs) mediate the majority of excitatory transmission in
the brain and enable the synaptic plasticity that underlies learning1. A diverse
array of AMPAR signalling complexes are established by receptor auxiliary subunits,
which associate with the AMPAR in various combinations to modulate trafficking,
gating and synaptic strength2. However, their mechanisms of action are poorly
understood. Here we determine cryo-electron microscopy structures of the heteromeric
GluA1–GluA2 receptor assembled with both TARP-γ8 and CNIH2, the predominant AMPAR
complex in the forebrain, in both resting and active states. Two TARP-γ8 and two
CNIH2 subunits insert at distinct sites beneath the ligand-binding domains of
the receptor, with site-specific lipids shaping each interaction and affecting
the gating regulation of the AMPARs. Activation of the receptor leads to asymmetry
between GluA1 and GluA2 along the ion conduction path and an outward expansion
of the channel triggers counter-rotations of both auxiliary subunit pairs, promoting
the active-state conformation. In addition, both TARP-γ8 and CNIH2 pivot towards
the pore exit upon activation, extending their reach for cytoplasmic receptor
elements. CNIH2 achieves this through its uniquely extended M2 helix, which has
transformed this endoplasmic reticulum-export factor into a powerful AMPAR modulator
that is capable of providing hippocampal pyramidal neurons with their integrative
synaptic properties. '
acknowledgement: We thank members of the Greger laboratory, B. Herguedas, J. Krieger
and J.-N. Dohrke for comments on the manuscript; J. Krieger and J.-N. Dohrke for
discussion, J. Krieger for help with the normal mode analysis, B. Köhegyi for help
with cryo-EM imaging, V. Chang and K. Suzuki for helping to generate the CNIH2-1D4-HA
stable cell line, M. Carvalho for assistance at early stages of this project, the
LMB scientific computing and the cryo-EM facility for support, P. Emsley for help
with model building, T. Nakane for helpful comments with RELION 3.1 and R. Warshamanage
for helping with EMDA cryo-EM-map processing. We acknowledge the Diamond Light Source
for access and support of the Cryo-EM facilities at the UK national electron bio10
imaging centre (eBIC), proposal EM17434, funded by the Wellcome Trust, MRC and BBSRC.
This work was supported by grants from the Medical Research Council, as part of
United Kingdom Research and Innovation (also known as UK Research and Innovation)
(MC_U105174197) and BBSRC (BB/N002113/1) to I.H.G.
article_processing_charge: No
article_type: original
author:
- first_name: Danyang
full_name: Zhang, Danyang
last_name: Zhang
- first_name: Jake
full_name: Watson, Jake
id: 63836096-4690-11EA-BD4E-32803DDC885E
last_name: Watson
orcid: 0000-0002-8698-3823
- first_name: Peter M.
full_name: Matthews, Peter M.
last_name: Matthews
- first_name: Ondrej
full_name: Cais, Ondrej
last_name: Cais
- first_name: Ingo H.
full_name: Greger, Ingo H.
last_name: Greger
citation:
ama: Zhang D, Watson J, Matthews PM, Cais O, Greger IH. Gating and modulation of
a hetero-octameric AMPA glutamate receptor. Nature. 2021;594:454-458. doi:10.1038/s41586-021-03613-0
apa: Zhang, D., Watson, J., Matthews, P. M., Cais, O., & Greger, I. H. (2021).
Gating and modulation of a hetero-octameric AMPA glutamate receptor. Nature.
Springer Nature. https://doi.org/10.1038/s41586-021-03613-0
chicago: Zhang, Danyang, Jake Watson, Peter M. Matthews, Ondrej Cais, and Ingo H.
Greger. “Gating and Modulation of a Hetero-Octameric AMPA Glutamate Receptor.”
Nature. Springer Nature, 2021. https://doi.org/10.1038/s41586-021-03613-0.
ieee: D. Zhang, J. Watson, P. M. Matthews, O. Cais, and I. H. Greger, “Gating and
modulation of a hetero-octameric AMPA glutamate receptor,” Nature, vol.
594. Springer Nature, pp. 454–458, 2021.
ista: Zhang D, Watson J, Matthews PM, Cais O, Greger IH. 2021. Gating and modulation
of a hetero-octameric AMPA glutamate receptor. Nature. 594, 454–458.
mla: Zhang, Danyang, et al. “Gating and Modulation of a Hetero-Octameric AMPA Glutamate
Receptor.” Nature, vol. 594, Springer Nature, 2021, pp. 454–58, doi:10.1038/s41586-021-03613-0.
short: D. Zhang, J. Watson, P.M. Matthews, O. Cais, I.H. Greger, Nature 594 (2021)
454–458.
date_created: 2021-06-13T22:01:33Z
date_published: 2021-06-02T00:00:00Z
date_updated: 2023-08-08T13:59:51Z
day: '02'
department:
- _id: PeJo
doi: 10.1038/s41586-021-03613-0
external_id:
isi:
- '000657238100003'
pmid:
- '34079129'
intvolume: ' 594'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1038/s41586-021-03613-0
month: '06'
oa: 1
oa_version: Published Version
page: 454-458
pmid: 1
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Gating and modulation of a hetero-octameric AMPA glutamate receptor
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 594
year: '2021'
...
---
_id: '9778'
abstract:
- lang: eng
text: The hippocampal mossy fiber synapse is a key synapse of the trisynaptic circuit.
Post-tetanic potentiation (PTP) is the most powerful form of plasticity at this
synaptic connection. It is widely believed that mossy fiber PTP is an entirely
presynaptic phenomenon, implying that PTP induction is input-specific, and requires
neither activity of multiple inputs nor stimulation of postsynaptic neurons. To
directly test cooperativity and associativity, we made paired recordings between
single mossy fiber terminals and postsynaptic CA3 pyramidal neurons in rat brain
slices. By stimulating non-overlapping mossy fiber inputs converging onto single
CA3 neurons, we confirm that PTP is input-specific and non-cooperative. Unexpectedly,
mossy fiber PTP exhibits anti-associative induction properties. EPSCs show only
minimal PTP after combined pre- and postsynaptic high-frequency stimulation with
intact postsynaptic Ca2+ signaling, but marked PTP in the absence of postsynaptic
spiking and after suppression of postsynaptic Ca2+ signaling (10 mM EGTA). PTP
is largely recovered by inhibitors of voltage-gated R- and L-type Ca2+ channels,
group II mGluRs, and vacuolar-type H+-ATPase, suggesting the involvement of retrograde
vesicular glutamate signaling. Transsynaptic regulation of PTP extends the repertoire
of synaptic computations, implementing a brake on mossy fiber detonation and a
“smart teacher” function of hippocampal mossy fiber synapses.
acknowledged_ssus:
- _id: SSU
acknowledgement: We thank Drs. Carolina Borges-Merjane and Jose Guzman for critically
reading the manuscript, and Pablo Castillo for discussions. We are grateful to Alois
Schlögl for help with analysis, Florian Marr for excellent technical assistance
and cell reconstruction, Christina Altmutter for technical help, Eleftheria Kralli-Beller
for manuscript editing, and the Scientific Service Units of IST Austria for support.
This project received funding from the European Research Council (ERC) under the
European Union’s Horizon 2020 research and innovation program (grant agreement No
692692) and the Fond zur Förderung der Wissenschaftlichen Forschung (Z 312-B27,
Wittgenstein award), both to P.J.
article_number: '2912'
article_processing_charge: No
article_type: original
author:
- first_name: David H
full_name: Vandael, David H
id: 3AE48E0A-F248-11E8-B48F-1D18A9856A87
last_name: Vandael
orcid: 0000-0001-7577-1676
- first_name: Yuji
full_name: Okamoto, Yuji
id: 3337E116-F248-11E8-B48F-1D18A9856A87
last_name: Okamoto
orcid: 0000-0003-0408-6094
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Vandael DH, Okamoto Y, Jonas PM. Transsynaptic modulation of presynaptic short-term
plasticity in hippocampal mossy fiber synapses. Nature Communications.
2021;12(1). doi:10.1038/s41467-021-23153-5
apa: Vandael, D. H., Okamoto, Y., & Jonas, P. M. (2021). Transsynaptic modulation
of presynaptic short-term plasticity in hippocampal mossy fiber synapses. Nature
Communications. Springer. https://doi.org/10.1038/s41467-021-23153-5
chicago: Vandael, David H, Yuji Okamoto, and Peter M Jonas. “Transsynaptic Modulation
of Presynaptic Short-Term Plasticity in Hippocampal Mossy Fiber Synapses.” Nature
Communications. Springer, 2021. https://doi.org/10.1038/s41467-021-23153-5.
ieee: D. H. Vandael, Y. Okamoto, and P. M. Jonas, “Transsynaptic modulation of presynaptic
short-term plasticity in hippocampal mossy fiber synapses,” Nature Communications,
vol. 12, no. 1. Springer, 2021.
ista: Vandael DH, Okamoto Y, Jonas PM. 2021. Transsynaptic modulation of presynaptic
short-term plasticity in hippocampal mossy fiber synapses. Nature Communications.
12(1), 2912.
mla: Vandael, David H., et al. “Transsynaptic Modulation of Presynaptic Short-Term
Plasticity in Hippocampal Mossy Fiber Synapses.” Nature Communications,
vol. 12, no. 1, 2912, Springer, 2021, doi:10.1038/s41467-021-23153-5.
short: D.H. Vandael, Y. Okamoto, P.M. Jonas, Nature Communications 12 (2021).
date_created: 2021-08-06T07:22:55Z
date_published: 2021-05-18T00:00:00Z
date_updated: 2023-08-10T14:16:16Z
day: '18'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1038/s41467-021-23153-5
ec_funded: 1
external_id:
isi:
- '000655481800014'
file:
- access_level: open_access
checksum: 6036a8cdae95e1707c2a04d54e325ff4
content_type: application/pdf
creator: kschuh
date_created: 2021-12-17T11:34:50Z
date_updated: 2021-12-17T11:34:50Z
file_id: '10563'
file_name: 2021_NatureCommunications_Vandael.pdf
file_size: 3108845
relation: main_file
success: 1
file_date_updated: 2021-12-17T11:34:50Z
has_accepted_license: '1'
intvolume: ' 12'
isi: 1
issue: '1'
keyword:
- general physics and astronomy
- general biochemistry
- genetics and molecular biology
- general chemistry
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: The Wittgenstein Prize
publication: Nature Communications
publication_identifier:
issn:
- 2041-1723
publication_status: published
publisher: Springer
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/synaptic-transmission-not-a-one-way-street/
scopus_import: '1'
status: public
title: Transsynaptic modulation of presynaptic short-term plasticity in hippocampal
mossy fiber synapses
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 12
year: '2021'
...
---
_id: '9985'
abstract:
- lang: eng
text: AMPA receptor (AMPAR) abundance and positioning at excitatory synapses regulates
the strength of transmission. Changes in AMPAR localisation can enact synaptic
plasticity, allowing long-term information storage, and is therefore tightly controlled.
Multiple mechanisms regulating AMPAR synaptic anchoring have been described, but
with limited coherence or comparison between reports, our understanding of this
process is unclear. Here, combining synaptic recordings from mouse hippocampal
slices and super-resolution imaging in dissociated cultures, we compare the contributions
of three AMPAR interaction domains controlling transmission at hippocampal CA1
synapses. We show that the AMPAR C-termini play only a modulatory role, whereas
the extracellular N-terminal domain (NTD) and PDZ interactions of the auxiliary
subunit TARP γ8 are both crucial, and each is sufficient to maintain transmission.
Our data support a model in which γ8 accumulates AMPARs at the postsynaptic density,
where the NTD further tunes their positioning. This interplay between cytosolic
(TARP γ8) and synaptic cleft (NTD) interactions provides versatility to regulate
synaptic transmission and plasticity.
acknowledgement: The authors are very grateful to Andrew Penn for advice and discussions
on surface receptor labelling in slice tissue, dissociated culture transfection,
and for providing tdTomato and BirAER expression plasmids. This work would not have
been possible without support from the Biological Services teams at both the Laboratory
of Molecular Biology and Ares facilities. We are also very grateful to Nick Barry
and Jerome Boulanger of the LMB Light Microscopy facility for support with confocal
and STORM imaging and analysis, Junichi Takagi for providing scFv-Clasp expression
constructs, Veronica Chang for assistance with scFv-Clasp protein production, and
Nejc Kejzar for assistance with cluster analysis. We would like to thank Teru Nakagawa
and Ole Paulsen for critical reading of the manuscript and constructive feedback.
This work was supported by grants from the Medical Research Council (MC_U105174197)
and BBSRC (BB/N002113/1).
article_number: '5083'
article_processing_charge: Yes
article_type: original
author:
- first_name: Jake
full_name: Watson, Jake
id: 63836096-4690-11EA-BD4E-32803DDC885E
last_name: Watson
orcid: 0000-0002-8698-3823
- first_name: Alexandra
full_name: Pinggera, Alexandra
last_name: Pinggera
- first_name: Hinze
full_name: Ho, Hinze
last_name: Ho
- first_name: Ingo H.
full_name: Greger, Ingo H.
last_name: Greger
citation:
ama: Watson J, Pinggera A, Ho H, Greger IH. AMPA receptor anchoring at CA1 synapses
is determined by N-terminal domain and TARP γ8 interactions. Nature Communications.
2021;12(1). doi:10.1038/s41467-021-25281-4
apa: Watson, J., Pinggera, A., Ho, H., & Greger, I. H. (2021). AMPA receptor
anchoring at CA1 synapses is determined by N-terminal domain and TARP γ8 interactions.
Nature Communications. Nature Publishing Group. https://doi.org/10.1038/s41467-021-25281-4
chicago: Watson, Jake, Alexandra Pinggera, Hinze Ho, and Ingo H. Greger. “AMPA Receptor
Anchoring at CA1 Synapses Is Determined by N-Terminal Domain and TARP Γ8 Interactions.”
Nature Communications. Nature Publishing Group, 2021. https://doi.org/10.1038/s41467-021-25281-4.
ieee: J. Watson, A. Pinggera, H. Ho, and I. H. Greger, “AMPA receptor anchoring
at CA1 synapses is determined by N-terminal domain and TARP γ8 interactions,”
Nature Communications, vol. 12, no. 1. Nature Publishing Group, 2021.
ista: Watson J, Pinggera A, Ho H, Greger IH. 2021. AMPA receptor anchoring at CA1
synapses is determined by N-terminal domain and TARP γ8 interactions. Nature Communications.
12(1), 5083.
mla: Watson, Jake, et al. “AMPA Receptor Anchoring at CA1 Synapses Is Determined
by N-Terminal Domain and TARP Γ8 Interactions.” Nature Communications,
vol. 12, no. 1, 5083, Nature Publishing Group, 2021, doi:10.1038/s41467-021-25281-4.
short: J. Watson, A. Pinggera, H. Ho, I.H. Greger, Nature Communications 12 (2021).
date_created: 2021-09-05T22:01:23Z
date_published: 2021-08-23T00:00:00Z
date_updated: 2023-08-11T11:07:51Z
day: '23'
ddc:
- '612'
department:
- _id: PeJo
doi: 10.1038/s41467-021-25281-4
external_id:
isi:
- '000687672000006'
pmid:
- '34426577 '
file:
- access_level: open_access
checksum: 1bf4f6a561f96bc426d754de9cb57710
content_type: application/pdf
creator: cchlebak
date_created: 2021-09-08T12:57:06Z
date_updated: 2021-09-08T12:57:06Z
file_id: '9991'
file_name: 2021_NatureCommunications_Watson.pdf
file_size: 18310502
relation: main_file
success: 1
file_date_updated: 2021-09-08T12:57:06Z
has_accepted_license: '1'
intvolume: ' 12'
isi: 1
issue: '1'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
publication: Nature Communications
publication_identifier:
eissn:
- 2041-1723
publication_status: published
publisher: Nature Publishing Group
quality_controlled: '1'
scopus_import: '1'
status: public
title: AMPA receptor anchoring at CA1 synapses is determined by N-terminal domain
and TARP γ8 interactions
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 12
year: '2021'
...
---
_id: '9438'
abstract:
- lang: eng
text: Rigorous investigation of synaptic transmission requires analysis of unitary
synaptic events by simultaneous recording from presynaptic terminals and postsynaptic
target neurons. However, this has been achieved at only a limited number of model
synapses, including the squid giant synapse and the mammalian calyx of Held. Cortical
presynaptic terminals have been largely inaccessible to direct presynaptic recording,
due to their small size. Here, we describe a protocol for improved subcellular
patch-clamp recording in rat and mouse brain slices, with the synapse in a largely
intact environment. Slice preparation takes ~2 h, recording ~3 h and post hoc
morphological analysis 2 d. Single presynaptic hippocampal mossy fiber terminals
are stimulated minimally invasively in the bouton-attached configuration, in which
the cytoplasmic content remains unperturbed, or in the whole-bouton configuration,
in which the cytoplasmic composition can be precisely controlled. Paired pre–postsynaptic
recordings can be integrated with biocytin labeling and morphological analysis,
allowing correlative investigation of synapse structure and function. Paired recordings
can be obtained from mossy fiber terminals in slices from both rats and mice,
implying applicability to genetically modified synapses. Paired recordings can
also be performed together with axon tract stimulation or optogenetic activation,
allowing comparison of unitary and compound synaptic events in the same target
cell. Finally, paired recordings can be combined with spontaneous event analysis,
permitting collection of miniature events generated at a single identified synapse.
In conclusion, the subcellular patch-clamp techniques detailed here should facilitate
analysis of biophysics, plasticity and circuit function of cortical synapses in
the mammalian central nervous system.
acknowledged_ssus:
- _id: M-Shop
acknowledgement: This project received funding from the European Research Council
(ERC) under the European Union’s Horizon 2020 research and innovation programme
(grant agreement no. 692692 to P.J.) and the Fond zur Förderung der Wissenschaftlichen
Forschung (Z 312-B27, Wittgenstein award to P.J., V 739-B27 to C.B.M.). We are grateful
to F. Marr and C. Altmutter for excellent technical assistance and cell reconstruction,
E. Kralli-Beller for manuscript editing, and the Scientific Service Units of IST
Austria, especially T. Asenov and Miba machine shop, for maximally efficient support.
article_processing_charge: No
article_type: original
author:
- first_name: David H
full_name: Vandael, David H
id: 3AE48E0A-F248-11E8-B48F-1D18A9856A87
last_name: Vandael
orcid: 0000-0001-7577-1676
- first_name: Yuji
full_name: Okamoto, Yuji
id: 3337E116-F248-11E8-B48F-1D18A9856A87
last_name: Okamoto
orcid: 0000-0003-0408-6094
- first_name: Carolina
full_name: Borges Merjane, Carolina
id: 4305C450-F248-11E8-B48F-1D18A9856A87
last_name: Borges Merjane
orcid: 0000-0003-0005-401X
- first_name: Victor M
full_name: Vargas Barroso, Victor M
id: 2F55A9DE-F248-11E8-B48F-1D18A9856A87
last_name: Vargas Barroso
- first_name: Benjamin
full_name: Suter, Benjamin
id: 4952F31E-F248-11E8-B48F-1D18A9856A87
last_name: Suter
orcid: 0000-0002-9885-6936
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Vandael DH, Okamoto Y, Borges Merjane C, Vargas Barroso VM, Suter B, Jonas
PM. Subcellular patch-clamp techniques for single-bouton stimulation and simultaneous
pre- and postsynaptic recording at cortical synapses. Nature Protocols.
2021;16(6):2947–2967. doi:10.1038/s41596-021-00526-0
apa: Vandael, D. H., Okamoto, Y., Borges Merjane, C., Vargas Barroso, V. M., Suter,
B., & Jonas, P. M. (2021). Subcellular patch-clamp techniques for single-bouton
stimulation and simultaneous pre- and postsynaptic recording at cortical synapses.
Nature Protocols. Springer Nature. https://doi.org/10.1038/s41596-021-00526-0
chicago: Vandael, David H, Yuji Okamoto, Carolina Borges Merjane, Victor M Vargas
Barroso, Benjamin Suter, and Peter M Jonas. “Subcellular Patch-Clamp Techniques
for Single-Bouton Stimulation and Simultaneous Pre- and Postsynaptic Recording
at Cortical Synapses.” Nature Protocols. Springer Nature, 2021. https://doi.org/10.1038/s41596-021-00526-0.
ieee: D. H. Vandael, Y. Okamoto, C. Borges Merjane, V. M. Vargas Barroso, B. Suter,
and P. M. Jonas, “Subcellular patch-clamp techniques for single-bouton stimulation
and simultaneous pre- and postsynaptic recording at cortical synapses,” Nature
Protocols, vol. 16, no. 6. Springer Nature, pp. 2947–2967, 2021.
ista: Vandael DH, Okamoto Y, Borges Merjane C, Vargas Barroso VM, Suter B, Jonas
PM. 2021. Subcellular patch-clamp techniques for single-bouton stimulation and
simultaneous pre- and postsynaptic recording at cortical synapses. Nature Protocols.
16(6), 2947–2967.
mla: Vandael, David H., et al. “Subcellular Patch-Clamp Techniques for Single-Bouton
Stimulation and Simultaneous Pre- and Postsynaptic Recording at Cortical Synapses.”
Nature Protocols, vol. 16, no. 6, Springer Nature, 2021, pp. 2947–2967,
doi:10.1038/s41596-021-00526-0.
short: D.H. Vandael, Y. Okamoto, C. Borges Merjane, V.M. Vargas Barroso, B. Suter,
P.M. Jonas, Nature Protocols 16 (2021) 2947–2967.
date_created: 2021-05-30T22:01:24Z
date_published: 2021-06-01T00:00:00Z
date_updated: 2023-08-10T22:30:51Z
day: '01'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1038/s41596-021-00526-0
ec_funded: 1
external_id:
isi:
- '000650528700003'
pmid:
- '33990799'
file:
- access_level: open_access
checksum: 7eb580abd8893cdb0b410cf41bc8c263
content_type: application/pdf
creator: cziletti
date_created: 2021-07-08T12:27:55Z
date_updated: 2021-12-02T23:30:05Z
embargo: 2021-12-01
file_id: '9639'
file_name: VandaeletalAuthorVersion2021.pdf
file_size: 38574802
relation: main_file
file_date_updated: 2021-12-02T23:30:05Z
has_accepted_license: '1'
intvolume: ' 16'
isi: 1
issue: '6'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Submitted Version
page: 2947–2967
pmid: 1
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: The Wittgenstein Prize
- _id: 2696E7FE-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: V00739
name: Structural plasticity at mossy fiber-CA3 synapses
publication: Nature Protocols
publication_identifier:
eissn:
- '17502799'
issn:
- '17542189'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Subcellular patch-clamp techniques for single-bouton stimulation and simultaneous
pre- and postsynaptic recording at cortical synapses
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 16
year: '2021'
...
---
_id: '10816'
abstract:
- lang: eng
text: Pattern separation is a fundamental brain computation that converts small
differences in input patterns into large differences in output patterns. Several
synaptic mechanisms of pattern separation have been proposed, including code expansion,
inhibition and plasticity; however, which of these mechanisms play a role in the
entorhinal cortex (EC)–dentate gyrus (DG)–CA3 circuit, a classical pattern separation
circuit, remains unclear. Here we show that a biologically realistic, full-scale
EC–DG–CA3 circuit model, including granule cells (GCs) and parvalbumin-positive
inhibitory interneurons (PV+-INs) in the DG, is an efficient pattern separator.
Both external gamma-modulated inhibition and internal lateral inhibition mediated
by PV+-INs substantially contributed to pattern separation. Both local connectivity
and fast signaling at GC–PV+-IN synapses were important for maximum effectiveness.
Similarly, mossy fiber synapses with conditional detonator properties contributed
to pattern separation. By contrast, perforant path synapses with Hebbian synaptic
plasticity and direct EC–CA3 connection shifted the network towards pattern completion.
Our results demonstrate that the specific properties of cells and synapses optimize
higher-order computations in biological networks and might be useful to improve
the deep learning capabilities of technical networks.
acknowledged_ssus:
- _id: SSU
acknowledgement: We thank A. Aertsen, N. Kopell, W. Maass, A. Roth, F. Stella and
T. Vogels for critically reading earlier versions of the manuscript. We are grateful
to F. Marr and C. Altmutter for excellent technical assistance, E. Kralli-Beller
for manuscript editing, and the Scientific Service Units of IST Austria for efficient
support. Finally, we thank T. Carnevale, L. Erdös, M. Hines, D. Nykamp and D. Schröder
for useful discussions, and R. Friedrich and S. Wiechert for sharing unpublished
data. This project received funding from the European Research Council (ERC) under
the European Union’s Horizon 2020 research and innovation programme (grant agreement
no. 692692, P.J.) and the Fond zur Förderung der Wissenschaftlichen Forschung (Z
312-B27, Wittgenstein award to P.J. and P 31815 to S.J.G.).
article_processing_charge: No
article_type: original
author:
- first_name: José
full_name: Guzmán, José
id: 30CC5506-F248-11E8-B48F-1D18A9856A87
last_name: Guzmán
orcid: 0000-0003-2209-5242
- first_name: Alois
full_name: Schlögl, Alois
id: 45BF87EE-F248-11E8-B48F-1D18A9856A87
last_name: Schlögl
orcid: 0000-0002-5621-8100
- first_name: 'Claudia '
full_name: 'Espinoza Martinez, Claudia '
id: 31FFEE2E-F248-11E8-B48F-1D18A9856A87
last_name: Espinoza Martinez
orcid: 0000-0003-4710-2082
- first_name: Xiaomin
full_name: Zhang, Xiaomin
id: 423EC9C2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
- first_name: Benjamin
full_name: Suter, Benjamin
id: 4952F31E-F248-11E8-B48F-1D18A9856A87
last_name: Suter
orcid: 0000-0002-9885-6936
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Guzmán J, Schlögl A, Espinoza Martinez C, Zhang X, Suter B, Jonas PM. How connectivity
rules and synaptic properties shape the efficacy of pattern separation in the
entorhinal cortex–dentate gyrus–CA3 network. Nature Computational Science.
2021;1(12):830-842. doi:10.1038/s43588-021-00157-1
apa: Guzmán, J., Schlögl, A., Espinoza Martinez, C., Zhang, X., Suter, B., &
Jonas, P. M. (2021). How connectivity rules and synaptic properties shape the
efficacy of pattern separation in the entorhinal cortex–dentate gyrus–CA3 network.
Nature Computational Science. Springer Nature. https://doi.org/10.1038/s43588-021-00157-1
chicago: Guzmán, José, Alois Schlögl, Claudia Espinoza Martinez, Xiaomin Zhang,
Benjamin Suter, and Peter M Jonas. “How Connectivity Rules and Synaptic Properties
Shape the Efficacy of Pattern Separation in the Entorhinal Cortex–Dentate Gyrus–CA3
Network.” Nature Computational Science. Springer Nature, 2021. https://doi.org/10.1038/s43588-021-00157-1.
ieee: J. Guzmán, A. Schlögl, C. Espinoza Martinez, X. Zhang, B. Suter, and P. M.
Jonas, “How connectivity rules and synaptic properties shape the efficacy of pattern
separation in the entorhinal cortex–dentate gyrus–CA3 network,” Nature Computational
Science, vol. 1, no. 12. Springer Nature, pp. 830–842, 2021.
ista: Guzmán J, Schlögl A, Espinoza Martinez C, Zhang X, Suter B, Jonas PM. 2021.
How connectivity rules and synaptic properties shape the efficacy of pattern separation
in the entorhinal cortex–dentate gyrus–CA3 network. Nature Computational Science.
1(12), 830–842.
mla: Guzmán, José, et al. “How Connectivity Rules and Synaptic Properties Shape
the Efficacy of Pattern Separation in the Entorhinal Cortex–Dentate Gyrus–CA3
Network.” Nature Computational Science, vol. 1, no. 12, Springer Nature,
2021, pp. 830–42, doi:10.1038/s43588-021-00157-1.
short: J. Guzmán, A. Schlögl, C. Espinoza Martinez, X. Zhang, B. Suter, P.M. Jonas,
Nature Computational Science 1 (2021) 830–842.
date_created: 2022-03-04T08:32:36Z
date_published: 2021-12-16T00:00:00Z
date_updated: 2023-08-10T22:30:10Z
day: '16'
ddc:
- '610'
department:
- _id: PeJo
doi: 10.1038/s43588-021-00157-1
ec_funded: 1
file:
- access_level: open_access
checksum: 9fec5b667909ef52be96d502e4f8c2ae
content_type: application/pdf
creator: patrickd
date_created: 2022-06-02T12:51:07Z
date_updated: 2022-06-18T22:30:03Z
embargo: 2022-06-17
file_id: '11430'
file_name: Guzmanetal2021.pdf
file_size: 1699466
relation: main_file
- access_level: open_access
checksum: 52a005b13a114e3c3a28fa6bbe8b1a8d
content_type: application/pdf
creator: patrickd
date_created: 2022-06-02T12:53:47Z
date_updated: 2022-06-18T22:30:03Z
embargo: 2022-06-17
file_id: '11431'
file_name: Guzmanetal2021Suppl.pdf
file_size: 3005651
relation: supplementary_material
title: Supplementary Material
file_date_updated: 2022-06-18T22:30:03Z
has_accepted_license: '1'
intvolume: ' 1'
issue: '12'
keyword:
- general medicine
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.biorxiv.org/content/10.1101/647800
month: '12'
oa: 1
oa_version: Submitted Version
page: 830-842
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: The Wittgenstein Prize
publication: Nature Computational Science
publication_identifier:
issn:
- 2662-8457
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- relation: press_release
url: https://ista.ac.at/en/news/spot-the-difference/
record:
- id: '10110'
relation: software
status: public
scopus_import: '1'
status: public
title: How connectivity rules and synaptic properties shape the efficacy of pattern
separation in the entorhinal cortex–dentate gyrus–CA3 network
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 1
year: '2021'
...
---
_id: '10110'
abstract:
- lang: eng
text: Pattern separation is a fundamental brain computation that converts small
differences in input patterns into large differences in output patterns. Several
synaptic mechanisms of pattern separation have been proposed, including code expansion,
inhibition and plasticity; however, which of these mechanisms play a role in the
entorhinal cortex (EC)–dentate gyrus (DG)–CA3 circuit, a classical pattern separation
circuit, remains unclear. Here we show that a biologically realistic, full-scale
EC–DG–CA3 circuit model, including granule cells (GCs) and parvalbumin-positive
inhibitory interneurons (PV+-INs) in the DG, is an efficient pattern separator.
Both external gamma-modulated inhibition and internal lateral inhibition mediated
by PV+-INs substantially contributed to pattern separation. Both local connectivity
and fast signaling at GC–PV+-IN synapses were important for maximum effectiveness.
Similarly, mossy fiber synapses with conditional detonator properties contributed
to pattern separation. By contrast, perforant path synapses with Hebbian synaptic
plasticity and direct EC–CA3 connection shifted the network towards pattern completion.
Our results demonstrate that the specific properties of cells and synapses optimize
higher-order computations in biological networks and might be useful to improve
the deep learning capabilities of technical networks.
author:
- first_name: José
full_name: Guzmán, José
id: 30CC5506-F248-11E8-B48F-1D18A9856A87
last_name: Guzmán
orcid: 0000-0003-2209-5242
- first_name: Alois
full_name: Schlögl, Alois
id: 45BF87EE-F248-11E8-B48F-1D18A9856A87
last_name: Schlögl
orcid: 0000-0002-5621-8100
- first_name: 'Claudia '
full_name: 'Espinoza Martinez, Claudia '
id: 31FFEE2E-F248-11E8-B48F-1D18A9856A87
last_name: Espinoza Martinez
orcid: 0000-0003-4710-2082
- first_name: Xiaomin
full_name: Zhang, Xiaomin
id: 423EC9C2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
- first_name: Benjamin
full_name: Suter, Benjamin
id: 4952F31E-F248-11E8-B48F-1D18A9856A87
last_name: Suter
orcid: 0000-0002-9885-6936
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Guzmán J, Schlögl A, Espinoza Martinez C, Zhang X, Suter B, Jonas PM. How connectivity
rules and synaptic properties shape the efficacy of pattern separation in the
entorhinal cortex–dentate gyrus–CA3 network. 2021. doi:10.15479/AT:ISTA:10110
apa: Guzmán, J., Schlögl, A., Espinoza Martinez, C., Zhang, X., Suter, B., &
Jonas, P. M. (2021). How connectivity rules and synaptic properties shape the
efficacy of pattern separation in the entorhinal cortex–dentate gyrus–CA3 network.
IST Austria. https://doi.org/10.15479/AT:ISTA:10110
chicago: Guzmán, José, Alois Schlögl, Claudia Espinoza Martinez, Xiaomin Zhang,
Benjamin Suter, and Peter M Jonas. “How Connectivity Rules and Synaptic Properties
Shape the Efficacy of Pattern Separation in the Entorhinal Cortex–Dentate Gyrus–CA3
Network.” IST Austria, 2021. https://doi.org/10.15479/AT:ISTA:10110.
ieee: J. Guzmán, A. Schlögl, C. Espinoza Martinez, X. Zhang, B. Suter, and P. M.
Jonas, “How connectivity rules and synaptic properties shape the efficacy of pattern
separation in the entorhinal cortex–dentate gyrus–CA3 network.” IST Austria, 2021.
ista: Guzmán J, Schlögl A, Espinoza Martinez C, Zhang X, Suter B, Jonas PM. 2021.
How connectivity rules and synaptic properties shape the efficacy of pattern separation
in the entorhinal cortex–dentate gyrus–CA3 network, IST Austria, 10.15479/AT:ISTA:10110.
mla: Guzmán, José, et al. How Connectivity Rules and Synaptic Properties Shape
the Efficacy of Pattern Separation in the Entorhinal Cortex–Dentate Gyrus–CA3
Network. IST Austria, 2021, doi:10.15479/AT:ISTA:10110.
short: J. Guzmán, A. Schlögl, C. Espinoza Martinez, X. Zhang, B. Suter, P.M. Jonas,
(2021).
date_created: 2021-10-08T06:44:22Z
date_published: 2021-12-16T00:00:00Z
date_updated: 2024-03-27T23:30:11Z
day: '16'
ddc:
- '005'
department:
- _id: PeJo
- _id: ScienComp
doi: 10.15479/AT:ISTA:10110
file:
- access_level: open_access
checksum: f92f8931cad0aa7e411c1715337bf408
content_type: application/x-zip-compressed
creator: cchlebak
date_created: 2021-10-08T08:46:04Z
date_updated: 2021-10-08T08:46:04Z
file_id: '10114'
file_name: patternseparation-main (1).zip
file_size: 332990101
relation: main_file
success: 1
file_date_updated: 2021-10-08T08:46:04Z
has_accepted_license: '1'
license: https://opensource.org/licenses/GPL-3.0
month: '12'
oa: 1
publisher: IST Austria
related_material:
link:
- description: News on IST Webpage
relation: press_release
url: https://ist.ac.at/en/news/spot-the-difference/
record:
- id: '10816'
relation: used_for_analysis_in
status: public
status: public
title: How connectivity rules and synaptic properties shape the efficacy of pattern
separation in the entorhinal cortex–dentate gyrus–CA3 network
tmp:
legal_code_url: https://www.gnu.org/licenses/gpl-3.0.en.html
name: GNU General Public License 3.0
short: GPL 3.0
type: software
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
year: '2021'
...
---
_id: '9437'
abstract:
- lang: eng
text: The synaptic connection from medial habenula (MHb) to interpeduncular nucleus
(IPN) is critical for emotion-related behaviors and uniquely expresses R-type
Ca2+ channels (Cav2.3) and auxiliary GABAB receptor (GBR) subunits, the K+-channel
tetramerization domain-containing proteins (KCTDs). Activation of GBRs facilitates
or inhibits transmitter release from MHb terminals depending on the IPN subnucleus,
but the role of KCTDs is unknown. We therefore examined the localization and function
of Cav2.3, GBRs, and KCTDs in this pathway in mice. We show in heterologous cells
that KCTD8 and KCTD12b directly bind to Cav2.3 and that KCTD8 potentiates Cav2.3
currents in the absence of GBRs. In the rostral IPN, KCTD8, KCTD12b, and Cav2.3
co-localize at the presynaptic active zone. Genetic deletion indicated a bidirectional
modulation of Cav2.3-mediated release by these KCTDs with a compensatory increase
of KCTD8 in the active zone in KCTD12b-deficient mice. The interaction of Cav2.3
with KCTDs therefore scales synaptic strength independent of GBR activation.
acknowledgement: We are grateful to Akari Hagiwara and Toshihisa Ohtsuka for CAST
antibody, and Masahiko Watanabe for neurexin antibody. We thank David Adams for
kindly providing the stable Cav2.3 cell line. Cav2.3 KO mice were kindly provided
by Tsutomu Tanabe. This project has received funding from the European Research
Council (ERC) and European Commission (EC), under the European Union’s Horizon 2020
research and innovation programme (ERC grant agreement no. 694539 to Ryuichi Shigemoto,
no. 692692 to Peter Jonas, and the Marie Skłodowska-Curie grant agreement no. 665385
to Cihan Önal), the Swiss National Science Foundation Grant 31003A-172881 to Bernhard
Bettler and Deutsche Forschungsgemeinschaft (For 2143) and BIOSS-2 to Akos Kulik.
article_number: e68274
article_processing_charge: No
article_type: original
author:
- first_name: Pradeep
full_name: Bhandari, Pradeep
id: 45EDD1BC-F248-11E8-B48F-1D18A9856A87
last_name: Bhandari
orcid: 0000-0003-0863-4481
- first_name: David H
full_name: Vandael, David H
id: 3AE48E0A-F248-11E8-B48F-1D18A9856A87
last_name: Vandael
orcid: 0000-0001-7577-1676
- first_name: Diego
full_name: Fernández-Fernández, Diego
last_name: Fernández-Fernández
- first_name: Thorsten
full_name: Fritzius, Thorsten
last_name: Fritzius
- first_name: David
full_name: Kleindienst, David
id: 42E121A4-F248-11E8-B48F-1D18A9856A87
last_name: Kleindienst
- first_name: Hüseyin C
full_name: Önal, Hüseyin C
id: 4659D740-F248-11E8-B48F-1D18A9856A87
last_name: Önal
orcid: 0000-0002-2771-2011
- first_name: Jacqueline-Claire
full_name: Montanaro-Punzengruber, Jacqueline-Claire
id: 3786AB44-F248-11E8-B48F-1D18A9856A87
last_name: Montanaro-Punzengruber
- first_name: Martin
full_name: Gassmann, Martin
last_name: Gassmann
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: Akos
full_name: Kulik, Akos
last_name: Kulik
- first_name: Bernhard
full_name: Bettler, Bernhard
last_name: Bettler
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Peter
full_name: Koppensteiner, Peter
id: 3B8B25A8-F248-11E8-B48F-1D18A9856A87
last_name: Koppensteiner
orcid: 0000-0002-3509-1948
citation:
ama: Bhandari P, Vandael DH, Fernández-Fernández D, et al. GABAB receptor auxiliary
subunits modulate Cav2.3-mediated release from medial habenula terminals. eLife.
2021;10. doi:10.7554/ELIFE.68274
apa: Bhandari, P., Vandael, D. H., Fernández-Fernández, D., Fritzius, T., Kleindienst,
D., Önal, H. C., … Koppensteiner, P. (2021). GABAB receptor auxiliary subunits
modulate Cav2.3-mediated release from medial habenula terminals. ELife.
eLife Sciences Publications. https://doi.org/10.7554/ELIFE.68274
chicago: Bhandari, Pradeep, David H Vandael, Diego Fernández-Fernández, Thorsten
Fritzius, David Kleindienst, Hüseyin C Önal, Jacqueline-Claire Montanaro-Punzengruber,
et al. “GABAB Receptor Auxiliary Subunits Modulate Cav2.3-Mediated Release from
Medial Habenula Terminals.” ELife. eLife Sciences Publications, 2021. https://doi.org/10.7554/ELIFE.68274.
ieee: P. Bhandari et al., “GABAB receptor auxiliary subunits modulate Cav2.3-mediated
release from medial habenula terminals,” eLife, vol. 10. eLife Sciences
Publications, 2021.
ista: Bhandari P, Vandael DH, Fernández-Fernández D, Fritzius T, Kleindienst D,
Önal HC, Montanaro-Punzengruber J-C, Gassmann M, Jonas PM, Kulik A, Bettler B,
Shigemoto R, Koppensteiner P. 2021. GABAB receptor auxiliary subunits modulate
Cav2.3-mediated release from medial habenula terminals. eLife. 10, e68274.
mla: Bhandari, Pradeep, et al. “GABAB Receptor Auxiliary Subunits Modulate Cav2.3-Mediated
Release from Medial Habenula Terminals.” ELife, vol. 10, e68274, eLife
Sciences Publications, 2021, doi:10.7554/ELIFE.68274.
short: P. Bhandari, D.H. Vandael, D. Fernández-Fernández, T. Fritzius, D. Kleindienst,
H.C. Önal, J.-C. Montanaro-Punzengruber, M. Gassmann, P.M. Jonas, A. Kulik, B.
Bettler, R. Shigemoto, P. Koppensteiner, ELife 10 (2021).
date_created: 2021-05-30T22:01:23Z
date_published: 2021-04-29T00:00:00Z
date_updated: 2024-03-27T23:30:30Z
day: '29'
ddc:
- '570'
department:
- _id: RySh
- _id: PeJo
doi: 10.7554/ELIFE.68274
ec_funded: 1
external_id:
isi:
- '000651761700001'
file:
- access_level: open_access
checksum: 6ebcb79999f889766f7cd79ee134ad28
content_type: application/pdf
creator: cziletti
date_created: 2021-05-31T09:43:09Z
date_updated: 2021-05-31T09:43:09Z
file_id: '9440'
file_name: 2021_eLife_Bhandari.pdf
file_size: 8174719
relation: main_file
success: 1
file_date_updated: 2021-05-31T09:43:09Z
has_accepted_license: '1'
intvolume: ' 10'
isi: 1
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
project:
- _id: 25CA28EA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '694539'
name: 'In situ analysis of single channel subunit composition in neurons: physiological
implication in synaptic plasticity and behaviour'
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
publication: eLife
publication_identifier:
eissn:
- 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
related_material:
link:
- relation: earlier_version
url: https://doi.org/10.1101/2020.04.16.045112
record:
- id: '9562'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: GABAB receptor auxiliary subunits modulate Cav2.3-mediated release from medial
habenula terminals
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 10
year: '2021'
...
---
_id: '8001'
abstract:
- lang: eng
text: Post-tetanic potentiation (PTP) is an attractive candidate mechanism for hippocampus-dependent
short-term memory. Although PTP has a uniquely large magnitude at hippocampal
mossy fiber-CA3 pyramidal neuron synapses, it is unclear whether it can be induced
by natural activity and whether its lifetime is sufficient to support short-term
memory. We combined in vivo recordings from granule cells (GCs), in vitro paired
recordings from mossy fiber terminals and postsynaptic CA3 neurons, and “flash
and freeze” electron microscopy. PTP was induced at single synapses and showed
a low induction threshold adapted to sparse GC activity in vivo. PTP was mainly
generated by enlargement of the readily releasable pool of synaptic vesicles,
allowing multiplicative interaction with other plasticity forms. PTP was associated
with an increase in the docked vesicle pool, suggesting formation of structural
“pool engrams.” Absence of presynaptic activity extended the lifetime of the potentiation,
enabling prolonged information storage in the hippocampal network.
acknowledged_ssus:
- _id: SSU
acknowledgement: This project received funding from the European Research Council
(ERC) under the European Union Horizon 2020 Research and Innovation Program (grant
agreement 692692 to P.J.) and the Fond zur Förderung der Wissenschaftlichen Forschung
( Z 312-B27 , Wittgenstein award to P.J. and V 739-B27 to C.B.-M.). We thank Drs.
Jozsef Csicsvari, Jose Guzman, Erwin Neher, and Ryuichi Shigemoto for commenting
on earlier versions of the manuscript. We are grateful to Walter Kaufmann, Daniel
Gütl, and Vanessa Zheden for EM training; Alois Schlögl for programming; Florian
Marr for excellent technical assistance and cell reconstruction; Christina Altmutter
for technical help; Eleftheria Kralli-Beller for manuscript editing; Taija Makinen
for providing the Prox1-CreERT2 mouse line; and the Scientific Service Units of
IST Austria for support.
article_processing_charge: No
article_type: original
author:
- first_name: David H
full_name: Vandael, David H
id: 3AE48E0A-F248-11E8-B48F-1D18A9856A87
last_name: Vandael
orcid: 0000-0001-7577-1676
- first_name: Carolina
full_name: Borges Merjane, Carolina
id: 4305C450-F248-11E8-B48F-1D18A9856A87
last_name: Borges Merjane
orcid: 0000-0003-0005-401X
- first_name: Xiaomin
full_name: Zhang, Xiaomin
id: 423EC9C2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Vandael DH, Borges Merjane C, Zhang X, Jonas PM. Short-term plasticity at hippocampal
mossy fiber synapses is induced by natural activity patterns and associated with
vesicle pool engram formation. Neuron. 2020;107(3):509-521. doi:10.1016/j.neuron.2020.05.013
apa: Vandael, D. H., Borges Merjane, C., Zhang, X., & Jonas, P. M. (2020). Short-term
plasticity at hippocampal mossy fiber synapses is induced by natural activity
patterns and associated with vesicle pool engram formation. Neuron. Elsevier.
https://doi.org/10.1016/j.neuron.2020.05.013
chicago: Vandael, David H, Carolina Borges Merjane, Xiaomin Zhang, and Peter M Jonas.
“Short-Term Plasticity at Hippocampal Mossy Fiber Synapses Is Induced by Natural
Activity Patterns and Associated with Vesicle Pool Engram Formation.” Neuron.
Elsevier, 2020. https://doi.org/10.1016/j.neuron.2020.05.013.
ieee: D. H. Vandael, C. Borges Merjane, X. Zhang, and P. M. Jonas, “Short-term plasticity
at hippocampal mossy fiber synapses is induced by natural activity patterns and
associated with vesicle pool engram formation,” Neuron, vol. 107, no. 3.
Elsevier, pp. 509–521, 2020.
ista: Vandael DH, Borges Merjane C, Zhang X, Jonas PM. 2020. Short-term plasticity
at hippocampal mossy fiber synapses is induced by natural activity patterns and
associated with vesicle pool engram formation. Neuron. 107(3), 509–521.
mla: Vandael, David H., et al. “Short-Term Plasticity at Hippocampal Mossy Fiber
Synapses Is Induced by Natural Activity Patterns and Associated with Vesicle Pool
Engram Formation.” Neuron, vol. 107, no. 3, Elsevier, 2020, pp. 509–21,
doi:10.1016/j.neuron.2020.05.013.
short: D.H. Vandael, C. Borges Merjane, X. Zhang, P.M. Jonas, Neuron 107 (2020)
509–521.
date_created: 2020-06-22T13:29:05Z
date_published: 2020-08-05T00:00:00Z
date_updated: 2023-08-22T07:45:25Z
day: '05'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1016/j.neuron.2020.05.013
ec_funded: 1
external_id:
isi:
- '000556135600004'
pmid:
- '32492366'
file:
- access_level: open_access
checksum: 4030b2be0c9625d54694a1e9fb00305e
content_type: application/pdf
creator: dernst
date_created: 2020-11-25T11:23:02Z
date_updated: 2020-11-25T11:23:02Z
file_id: '8811'
file_name: 2020_Neuron_Vandael.pdf
file_size: 4390833
relation: main_file
success: 1
file_date_updated: 2020-11-25T11:23:02Z
has_accepted_license: '1'
intvolume: ' 107'
isi: 1
issue: '3'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
page: 509-521
pmid: 1
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: The Wittgenstein Prize
- _id: 2696E7FE-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: V00739
name: Structural plasticity at mossy fiber-CA3 synapses
publication: Neuron
publication_identifier:
eissn:
- '10974199'
issn:
- 0896-6273
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/possible-physical-trace-of-short-term-memory-found/
scopus_import: '1'
status: public
title: Short-term plasticity at hippocampal mossy fiber synapses is induced by natural
activity patterns and associated with vesicle pool engram formation
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 107
year: '2020'
...
---
_id: '8261'
abstract:
- lang: eng
text: Dentate gyrus granule cells (GCs) connect the entorhinal cortex to the hippocampal
CA3 region, but how they process spatial information remains enigmatic. To examine
the role of GCs in spatial coding, we measured excitatory postsynaptic potentials
(EPSPs) and action potentials (APs) in head-fixed mice running on a linear belt.
Intracellular recording from morphologically identified GCs revealed that most
cells were active, but activity level varied over a wide range. Whereas only ∼5%
of GCs showed spatially tuned spiking, ∼50% received spatially tuned input. Thus,
the GC population broadly encodes spatial information, but only a subset relays
this information to the CA3 network. Fourier analysis indicated that GCs received
conjunctive place-grid-like synaptic input, suggesting code conversion in single
neurons. GC firing was correlated with dendritic complexity and intrinsic excitability,
but not extrinsic excitatory input or dendritic cable properties. Thus, functional
maturation may control input-output transformation and spatial code conversion.
acknowledged_ssus:
- _id: M-Shop
- _id: ScienComp
- _id: PreCl
acknowledgement: This project has received funding from the European Research Council
(ERC) under the European Union’s Horizon 2020 research and innovation program (grant
agreement 692692, P.J.) and the Fond zur Förderung der Wissenschaftlichen Forschung
(Z 312-B27, Wittgenstein award, P.J.). We thank Gyorgy Buzsáki, Jozsef Csicsvari,
Juan Ramirez Villegas, and Federico Stella for commenting on earlier versions of
this manuscript. We also thank Katie Bittner, Michael Brecht, Albert Lee, Jeffery
Magee, and Alejandro Pernía-Andrade for sharing expertise in in vivo patch-clamp
recording. We are grateful to Florian Marr for cell labeling, cell reconstruction,
and technical assistance; Ben Suter for helpful discussions; Christina Altmutter
for technical support; Eleftheria Kralli-Beller for manuscript editing; and Todor
Asenov (Machine Shop) for device construction. We also thank the Scientific Service
Units (SSUs) of IST Austria (Machine Shop, Scientific Computing, and Preclinical
Facility) for efficient support.
article_processing_charge: No
article_type: original
author:
- first_name: Xiaomin
full_name: Zhang, Xiaomin
id: 423EC9C2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
- first_name: Alois
full_name: Schlögl, Alois
id: 45BF87EE-F248-11E8-B48F-1D18A9856A87
last_name: Schlögl
orcid: 0000-0002-5621-8100
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Zhang X, Schlögl A, Jonas PM. Selective routing of spatial information flow
from input to output in hippocampal granule cells. Neuron. 2020;107(6):1212-1225.
doi:10.1016/j.neuron.2020.07.006
apa: Zhang, X., Schlögl, A., & Jonas, P. M. (2020). Selective routing of spatial
information flow from input to output in hippocampal granule cells. Neuron.
Elsevier. https://doi.org/10.1016/j.neuron.2020.07.006
chicago: Zhang, Xiaomin, Alois Schlögl, and Peter M Jonas. “Selective Routing of
Spatial Information Flow from Input to Output in Hippocampal Granule Cells.” Neuron.
Elsevier, 2020. https://doi.org/10.1016/j.neuron.2020.07.006.
ieee: X. Zhang, A. Schlögl, and P. M. Jonas, “Selective routing of spatial information
flow from input to output in hippocampal granule cells,” Neuron, vol. 107,
no. 6. Elsevier, pp. 1212–1225, 2020.
ista: Zhang X, Schlögl A, Jonas PM. 2020. Selective routing of spatial information
flow from input to output in hippocampal granule cells. Neuron. 107(6), 1212–1225.
mla: Zhang, Xiaomin, et al. “Selective Routing of Spatial Information Flow from
Input to Output in Hippocampal Granule Cells.” Neuron, vol. 107, no. 6,
Elsevier, 2020, pp. 1212–25, doi:10.1016/j.neuron.2020.07.006.
short: X. Zhang, A. Schlögl, P.M. Jonas, Neuron 107 (2020) 1212–1225.
date_created: 2020-08-14T09:36:05Z
date_published: 2020-09-23T00:00:00Z
date_updated: 2023-08-22T08:30:55Z
day: '23'
ddc:
- '570'
department:
- _id: PeJo
- _id: ScienComp
doi: 10.1016/j.neuron.2020.07.006
ec_funded: 1
external_id:
isi:
- '000579698700009'
pmid:
- '32763145'
file:
- access_level: open_access
checksum: 44a5960fc083a4cb3488d22224859fdc
content_type: application/pdf
creator: dernst
date_created: 2020-12-04T09:29:21Z
date_updated: 2020-12-04T09:29:21Z
file_id: '8920'
file_name: 2020_Neuron_Zhang.pdf
file_size: 3011120
relation: main_file
success: 1
file_date_updated: 2020-12-04T09:29:21Z
has_accepted_license: '1'
intvolume: ' 107'
isi: 1
issue: '6'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
page: 1212-1225
pmid: 1
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: The Wittgenstein Prize
publication: Neuron
publication_identifier:
issn:
- 0896-6273
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
link:
- description: News on IST Website
relation: press_release
url: https://ist.ac.at/en/news/the-bouncer-in-the-brain/
status: public
title: Selective routing of spatial information flow from input to output in hippocampal
granule cells
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 107
year: '2020'
...
---
_id: '7473'
abstract:
- lang: eng
text: How structural and functional properties of synapses relate to each other
is a fundamental question in neuroscience. Electrophysiology has elucidated mechanisms
of synaptic transmission, and electron microscopy (EM) has provided insight into
morphological properties of synapses. Here we describe an enhanced method for
functional EM (“flash and freeze”), combining optogenetic stimulation with high-pressure
freezing. We demonstrate that the improved method can be applied to intact networks
in acute brain slices and organotypic slice cultures from mice. As a proof of
concept, we probed vesicle pool changes during synaptic transmission at the hippocampal
mossy fiber-CA3 pyramidal neuron synapse. Our findings show overlap of the docked
vesicle pool and the functionally defined readily releasable pool and provide
evidence of fast endocytosis at this synapse. Functional EM with acute slices
and slice cultures has the potential to reveal the structural and functional mechanisms
of transmission in intact, genetically perturbed, and disease-affected synapses.
acknowledgement: This project has received funding from the European Research Council
(ERC) and European Commission (EC), under the European Union’s Horizon 2020 research
and innovation programme (ERC grant agreement No. 692692 and Marie Sklodowska-Curie
708497) and from Fonds zur Förderung der Wissenschaftlichen Forschung (Z 312-B27
Wittgenstein award and DK W1205-B09). We thank Johann Danzl and Ryuichi Shigemoto
for critically reading the manuscript; Walter Kaufmann, Daniel Gutl, and Vanessa
Zheden for extensive EM training, advice, and experimental assistance; Benjamin
Suter for substantial help with light stimulation, ImageJ plugins for analysis,
and manuscript editing; Florian Marr and Christina Altmutter for technical support;
Eleftheria Kralli-Beller for manuscript editing; Julia König and Paul Wurzinger
(Leica Microsystems) for helpful technical discussions; and Taija Makinen for providing
the Prox1-CreERT2 mouse line.
article_processing_charge: No
article_type: original
author:
- first_name: Carolina
full_name: Borges Merjane, Carolina
id: 4305C450-F248-11E8-B48F-1D18A9856A87
last_name: Borges Merjane
orcid: 0000-0003-0005-401X
- first_name: Olena
full_name: Kim, Olena
id: 3F8ABDDA-F248-11E8-B48F-1D18A9856A87
last_name: Kim
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Borges Merjane C, Kim O, Jonas PM. Functional electron microscopy (“Flash and
Freeze”) of identified cortical synapses in acute brain slices. Neuron.
2020;105:992-1006. doi:10.1016/j.neuron.2019.12.022
apa: Borges Merjane, C., Kim, O., & Jonas, P. M. (2020). Functional electron
microscopy (“Flash and Freeze”) of identified cortical synapses in acute brain
slices. Neuron. Elsevier. https://doi.org/10.1016/j.neuron.2019.12.022
chicago: Borges Merjane, Carolina, Olena Kim, and Peter M Jonas. “Functional Electron
Microscopy (‘Flash and Freeze’) of Identified Cortical Synapses in Acute Brain
Slices.” Neuron. Elsevier, 2020. https://doi.org/10.1016/j.neuron.2019.12.022.
ieee: C. Borges Merjane, O. Kim, and P. M. Jonas, “Functional electron microscopy
(‘Flash and Freeze’) of identified cortical synapses in acute brain slices,” Neuron,
vol. 105. Elsevier, pp. 992–1006, 2020.
ista: Borges Merjane C, Kim O, Jonas PM. 2020. Functional electron microscopy (“Flash
and Freeze”) of identified cortical synapses in acute brain slices. Neuron. 105,
992–1006.
mla: Borges Merjane, Carolina, et al. “Functional Electron Microscopy (‘Flash and
Freeze’) of Identified Cortical Synapses in Acute Brain Slices.” Neuron,
vol. 105, Elsevier, 2020, pp. 992–1006, doi:10.1016/j.neuron.2019.12.022.
short: C. Borges Merjane, O. Kim, P.M. Jonas, Neuron 105 (2020) 992–1006.
date_created: 2020-02-10T15:59:45Z
date_published: 2020-03-18T00:00:00Z
date_updated: 2024-03-27T23:30:07Z
day: '18'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1016/j.neuron.2019.12.022
ec_funded: 1
external_id:
isi:
- '000520854700008'
pmid:
- '31928842'
file:
- access_level: open_access
checksum: 3582664addf26859e86ac5bec3e01416
content_type: application/pdf
creator: dernst
date_created: 2020-11-20T08:58:53Z
date_updated: 2020-11-20T08:58:53Z
file_id: '8778'
file_name: 2020_Neuron_BorgesMerjane.pdf
file_size: 9712957
relation: main_file
success: 1
file_date_updated: 2020-11-20T08:58:53Z
has_accepted_license: '1'
intvolume: ' 105'
isi: 1
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
page: 992-1006
pmid: 1
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: 25BAF7B2-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '708497'
name: Presynaptic calcium channels distribution and impact on coupling at the hippocampal
mossy fiber synapse
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: The Wittgenstein Prize
- _id: 25C3DBB6-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: W01205
name: Zellkommunikation in Gesundheit und Krankheit
publication: Neuron
publication_identifier:
issn:
- 0896-6273
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/flash-and-freeze-reveals-dynamics-of-nerve-connections/
record:
- id: '11196'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Functional electron microscopy (“Flash and Freeze”) of identified cortical
synapses in acute brain slices
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 105
year: '2020'
...
---
_id: '7405'
abstract:
- lang: eng
text: Biophysical modeling of neuronal networks helps to integrate and interpret
rapidly growing and disparate experimental datasets at multiple scales. The NetPyNE
tool (www.netpyne.org) provides both programmatic and graphical interfaces to
develop data-driven multiscale network models in NEURON. NetPyNE clearly separates
model parameters from implementation code. Users provide specifications at a high
level via a standardized declarative language, for example connectivity rules,
to create millions of cell-to-cell connections. NetPyNE then enables users to
generate the NEURON network, run efficiently parallelized simulations, optimize
and explore network parameters through automated batch runs, and use built-in
functions for visualization and analysis – connectivity matrices, voltage traces,
spike raster plots, local field potentials, and information theoretic measures.
NetPyNE also facilitates model sharing by exporting and importing standardized
formats (NeuroML and SONATA). NetPyNE is already being used to teach computational
neuroscience students and by modelers to investigate brain regions and phenomena.
article_number: e44494
article_processing_charge: No
article_type: original
author:
- first_name: Salvador
full_name: Dura-Bernal, Salvador
last_name: Dura-Bernal
- first_name: Benjamin
full_name: Suter, Benjamin
id: 4952F31E-F248-11E8-B48F-1D18A9856A87
last_name: Suter
orcid: 0000-0002-9885-6936
- first_name: Padraig
full_name: Gleeson, Padraig
last_name: Gleeson
- first_name: Matteo
full_name: Cantarelli, Matteo
last_name: Cantarelli
- first_name: Adrian
full_name: Quintana, Adrian
last_name: Quintana
- first_name: Facundo
full_name: Rodriguez, Facundo
last_name: Rodriguez
- first_name: David J
full_name: Kedziora, David J
last_name: Kedziora
- first_name: George L
full_name: Chadderdon, George L
last_name: Chadderdon
- first_name: Cliff C
full_name: Kerr, Cliff C
last_name: Kerr
- first_name: Samuel A
full_name: Neymotin, Samuel A
last_name: Neymotin
- first_name: Robert A
full_name: McDougal, Robert A
last_name: McDougal
- first_name: Michael
full_name: Hines, Michael
last_name: Hines
- first_name: Gordon MG
full_name: Shepherd, Gordon MG
last_name: Shepherd
- first_name: William W
full_name: Lytton, William W
last_name: Lytton
citation:
ama: Dura-Bernal S, Suter B, Gleeson P, et al. NetPyNE, a tool for data-driven multiscale
modeling of brain circuits. eLife. 2019;8. doi:10.7554/elife.44494
apa: Dura-Bernal, S., Suter, B., Gleeson, P., Cantarelli, M., Quintana, A., Rodriguez,
F., … Lytton, W. W. (2019). NetPyNE, a tool for data-driven multiscale modeling
of brain circuits. ELife. eLife Sciences Publications. https://doi.org/10.7554/elife.44494
chicago: Dura-Bernal, Salvador, Benjamin Suter, Padraig Gleeson, Matteo Cantarelli,
Adrian Quintana, Facundo Rodriguez, David J Kedziora, et al. “NetPyNE, a Tool
for Data-Driven Multiscale Modeling of Brain Circuits.” ELife. eLife Sciences
Publications, 2019. https://doi.org/10.7554/elife.44494.
ieee: S. Dura-Bernal et al., “NetPyNE, a tool for data-driven multiscale
modeling of brain circuits,” eLife, vol. 8. eLife Sciences Publications,
2019.
ista: Dura-Bernal S, Suter B, Gleeson P, Cantarelli M, Quintana A, Rodriguez F,
Kedziora DJ, Chadderdon GL, Kerr CC, Neymotin SA, McDougal RA, Hines M, Shepherd
GM, Lytton WW. 2019. NetPyNE, a tool for data-driven multiscale modeling of brain
circuits. eLife. 8, e44494.
mla: Dura-Bernal, Salvador, et al. “NetPyNE, a Tool for Data-Driven Multiscale Modeling
of Brain Circuits.” ELife, vol. 8, e44494, eLife Sciences Publications,
2019, doi:10.7554/elife.44494.
short: S. Dura-Bernal, B. Suter, P. Gleeson, M. Cantarelli, A. Quintana, F. Rodriguez,
D.J. Kedziora, G.L. Chadderdon, C.C. Kerr, S.A. Neymotin, R.A. McDougal, M. Hines,
G.M. Shepherd, W.W. Lytton, ELife 8 (2019).
date_created: 2020-01-30T09:08:01Z
date_published: 2019-05-31T00:00:00Z
date_updated: 2023-09-07T14:27:52Z
day: '31'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.7554/elife.44494
external_id:
isi:
- '000468968400001'
pmid:
- '31025934'
file:
- access_level: open_access
checksum: 7014189c11c10a12feeeae37f054871d
content_type: application/pdf
creator: dernst
date_created: 2020-02-04T08:41:47Z
date_updated: 2020-07-14T12:47:57Z
file_id: '7444'
file_name: 2019_eLife_DuraBernal.pdf
file_size: 6182359
relation: main_file
file_date_updated: 2020-07-14T12:47:57Z
has_accepted_license: '1'
intvolume: ' 8'
isi: 1
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
pmid: 1
publication: eLife
publication_identifier:
issn:
- 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: NetPyNE, a tool for data-driven multiscale modeling of brain circuits
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 8
year: '2019'
...
---
_id: '11222'
acknowledgement: This work was supported by the ERC and EU Horizon 2020 (ERC 692692;
MSC-IF 708497) and FWF Z 312-B27 Wittgenstein award; W 1205-B09).
article_number: A3.27
article_processing_charge: No
author:
- first_name: Olena
full_name: Kim, Olena
id: 3F8ABDDA-F248-11E8-B48F-1D18A9856A87
last_name: Kim
- first_name: Carolina
full_name: Borges Merjane, Carolina
id: 4305C450-F248-11E8-B48F-1D18A9856A87
last_name: Borges Merjane
orcid: 0000-0003-0005-401X
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: 'Kim O, Borges Merjane C, Jonas PM. Functional analysis of the docked vesicle
pool in hippocampal mossy fiber terminals by electron microscopy. In: Intrinsic
Activity. Vol 7. Austrian Pharmacological Society; 2019. doi:10.25006/ia.7.s1-a3.27'
apa: 'Kim, O., Borges Merjane, C., & Jonas, P. M. (2019). Functional analysis
of the docked vesicle pool in hippocampal mossy fiber terminals by electron microscopy.
In Intrinsic Activity (Vol. 7). Innsbruck, Austria: Austrian Pharmacological
Society. https://doi.org/10.25006/ia.7.s1-a3.27'
chicago: Kim, Olena, Carolina Borges Merjane, and Peter M Jonas. “Functional Analysis
of the Docked Vesicle Pool in Hippocampal Mossy Fiber Terminals by Electron Microscopy.”
In Intrinsic Activity, Vol. 7. Austrian Pharmacological Society, 2019.
https://doi.org/10.25006/ia.7.s1-a3.27.
ieee: O. Kim, C. Borges Merjane, and P. M. Jonas, “Functional analysis of the docked
vesicle pool in hippocampal mossy fiber terminals by electron microscopy,” in
Intrinsic Activity, Innsbruck, Austria, 2019, vol. 7, no. Suppl. 1.
ista: 'Kim O, Borges Merjane C, Jonas PM. 2019. Functional analysis of the docked
vesicle pool in hippocampal mossy fiber terminals by electron microscopy. Intrinsic
Activity. ANA: Austrian Neuroscience Association ; APHAR: Austrian Pharmacological
Society vol. 7, A3.27.'
mla: Kim, Olena, et al. “Functional Analysis of the Docked Vesicle Pool in Hippocampal
Mossy Fiber Terminals by Electron Microscopy.” Intrinsic Activity, vol.
7, no. Suppl. 1, A3.27, Austrian Pharmacological Society, 2019, doi:10.25006/ia.7.s1-a3.27.
short: O. Kim, C. Borges Merjane, P.M. Jonas, in:, Intrinsic Activity, Austrian
Pharmacological Society, 2019.
conference:
end_date: 2019-09-27
location: Innsbruck, Austria
name: 'ANA: Austrian Neuroscience Association ; APHAR: Austrian Pharmacological
Society'
start_date: 2019-09-25
date_created: 2022-04-20T15:06:05Z
date_published: 2019-09-11T00:00:00Z
date_updated: 2024-03-27T23:30:07Z
day: '11'
department:
- _id: PeJo
doi: 10.25006/ia.7.s1-a3.27
ec_funded: 1
intvolume: ' 7'
issue: Suppl. 1
keyword:
- hippocampus
- mossy fibers
- readily releasable pool
- electron microscopy
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.intrinsicactivity.org/2019/7/S1/A3.27/
month: '09'
oa: 1
oa_version: Published Version
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: 25BAF7B2-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '708497'
name: Presynaptic calcium channels distribution and impact on coupling at the hippocampal
mossy fiber synapse
- _id: 25C3DBB6-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: W01205
name: Zellkommunikation in Gesundheit und Krankheit
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: The Wittgenstein Prize
publication: Intrinsic Activity
publication_identifier:
issn:
- 2309-8503
publication_status: published
publisher: Austrian Pharmacological Society
quality_controlled: '1'
related_material:
record:
- id: '11196'
relation: dissertation_contains
status: public
status: public
title: Functional analysis of the docked vesicle pool in hippocampal mossy fiber terminals
by electron microscopy
type: conference_abstract
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
volume: 7
year: '2019'
...
---
_id: '6363'
abstract:
- lang: eng
text: "Distinguishing between similar experiences is achieved by the brain
\ in a process called pattern separation. In the hippocampus, pattern
\ separation reduces the interference of memories and increases the storage
capacity by decorrelating similar inputs patterns of neuronal activity into
\ non-overlapping output firing patterns. Winners-take-all (WTA) mechanism
\ is a theoretical model for pattern separation in which a \"winner\"
\ cell suppresses the activity of the neighboring neurons through feedback
inhibition. However, if the network properties of the dentate gyrus support WTA
as a biologically conceivable model remains unknown. Here, we showed that the
connectivity rules of PV+interneurons and their synaptic properties are optimizedfor
efficient pattern separation. We found using multiple whole-cell in vitrorecordings
that PV+interneurons mainly connect to granule cells (GC) through lateral inhibition,
a form of feedback inhibition in which a GC inhibits other GCs but not
\ itself through the activation of PV+interneurons. Thus, lateral inhibition
between GC–PV+interneurons was ~10 times more abundant than recurrent connections.
Furthermore, the GC–PV+interneuron connectivity was more spatially confined
\ but less abundant than PV+interneurons–GC connectivity, leading to an
\ asymmetrical distribution of excitatory and inhibitory connectivity. Our
network model of the dentate gyrus with incorporated real connectivity rules efficiently
decorrelates neuronal activity patterns using WTA as the primary mechanism.
\ This process relied on lateral inhibition, fast-signaling properties of
\ PV+interneurons and the asymmetrical distribution of excitatory and inhibitory
connectivity. Finally, we found that silencing the activity of PV+interneurons
in vivoleads to acute deficits in discrimination between similar environments,
suggesting that PV+interneuron networks are necessary for behavioral relevant
computations. Our results demonstrate that PV+interneurons possess unique
connectivity and fast signaling properties that confer to the dentate
\ gyrus network properties that allow the emergence of pattern separation. Thus,
our results contribute to the knowledge of how specific forms of network organization
underlie sophisticated types of information processing. \r\n"
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: 'Claudia '
full_name: 'Espinoza Martinez, Claudia '
id: 31FFEE2E-F248-11E8-B48F-1D18A9856A87
last_name: Espinoza Martinez
orcid: 0000-0003-4710-2082
citation:
ama: Espinoza Martinez C. Parvalbumin+ interneurons enable efficient pattern separation
in hippocampal microcircuits. 2019. doi:10.15479/AT:ISTA:6363
apa: Espinoza Martinez, C. (2019). Parvalbumin+ interneurons enable efficient
pattern separation in hippocampal microcircuits. Institute of Science and
Technology Austria. https://doi.org/10.15479/AT:ISTA:6363
chicago: Espinoza Martinez, Claudia . “Parvalbumin+ Interneurons Enable Efficient
Pattern Separation in Hippocampal Microcircuits.” Institute of Science and Technology
Austria, 2019. https://doi.org/10.15479/AT:ISTA:6363.
ieee: C. Espinoza Martinez, “Parvalbumin+ interneurons enable efficient pattern
separation in hippocampal microcircuits,” Institute of Science and Technology
Austria, 2019.
ista: Espinoza Martinez C. 2019. Parvalbumin+ interneurons enable efficient pattern
separation in hippocampal microcircuits. Institute of Science and Technology Austria.
mla: Espinoza Martinez, Claudia. Parvalbumin+ Interneurons Enable Efficient Pattern
Separation in Hippocampal Microcircuits. Institute of Science and Technology
Austria, 2019, doi:10.15479/AT:ISTA:6363.
short: C. Espinoza Martinez, Parvalbumin+ Interneurons Enable Efficient Pattern
Separation in Hippocampal Microcircuits, Institute of Science and Technology Austria,
2019.
date_created: 2019-04-30T11:56:10Z
date_published: 2019-04-30T00:00:00Z
date_updated: 2023-09-15T12:03:48Z
day: '30'
ddc:
- '570'
degree_awarded: PhD
department:
- _id: PeJo
doi: 10.15479/AT:ISTA:6363
file:
- access_level: open_access
checksum: 77c6c05cfe8b58c8abcf1b854375d084
content_type: application/pdf
creator: cespinoza
date_created: 2019-05-07T16:00:39Z
date_updated: 2021-02-11T11:17:15Z
embargo: 2020-05-09
file_id: '6389'
file_name: Espinozathesis_all2.pdf
file_size: 13966891
relation: main_file
- access_level: closed
checksum: f6aa819f127691a2b0fc21c76eb09746
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: cespinoza
date_created: 2019-05-07T16:00:48Z
date_updated: 2020-07-14T12:47:28Z
embargo_to: open_access
file_id: '6390'
file_name: Espinoza_Thesis.docx
file_size: 11159900
relation: source_file
file_date_updated: 2021-02-11T11:17:15Z
has_accepted_license: '1'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
page: '140'
publication_identifier:
isbn:
- 978-3-99078-000-8
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '21'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
title: Parvalbumin+ interneurons enable efficient pattern separation in hippocampal
microcircuits
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2019'
...
---
_id: '320'
abstract:
- lang: eng
text: 'Fast-spiking, parvalbumin-expressing GABAergic interneurons (PV+-BCs) express
a complex machinery of rapid signaling mechanisms, including specialized voltage-gated
ion channels to generate brief action potentials (APs). However, short APs are
associated with overlapping Na+ and K+ fluxes and are therefore energetically
expensive. How the potentially vicious combination of high AP frequency and inefficient
spike generation can be reconciled with limited energy supply is presently unclear.
To address this question, we performed direct recordings from the PV+-BC axon,
the subcellular structure where active conductances for AP initiation and propagation
are located. Surprisingly, the energy required for the AP was, on average, only
∼1.6 times the theoretical minimum. High energy efficiency emerged from the combination
of fast inactivation of Na+ channels and delayed activation of Kv3-type K+ channels,
which minimized ion flux overlap during APs. Thus, the complementary tuning of
axonal Na+ and K+ channel gating optimizes both fast signaling properties and
metabolic efficiency. Hu et al. demonstrate that action potentials in parvalbumin-expressing
GABAergic interneuron axons are energetically efficient, which is highly unexpected
given their brief duration. High energy efficiency emerges from the combination
of fast inactivation of voltage-gated Na+ channels and delayed activation of Kv3
channels in the axon. '
article_processing_charge: Yes (in subscription journal)
author:
- first_name: Hua
full_name: Hu, Hua
id: 4AC0145C-F248-11E8-B48F-1D18A9856A87
last_name: Hu
- first_name: Fabian
full_name: Roth, Fabian
last_name: Roth
- first_name: David H
full_name: Vandael, David H
id: 3AE48E0A-F248-11E8-B48F-1D18A9856A87
last_name: Vandael
orcid: 0000-0001-7577-1676
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Hu H, Roth F, Vandael DH, Jonas PM. Complementary tuning of Na+ and K+ channel
gating underlies fast and energy-efficient action potentials in GABAergic interneuron
axons. Neuron. 2018;98(1):156-165. doi:10.1016/j.neuron.2018.02.024
apa: Hu, H., Roth, F., Vandael, D. H., & Jonas, P. M. (2018). Complementary
tuning of Na+ and K+ channel gating underlies fast and energy-efficient action
potentials in GABAergic interneuron axons. Neuron. Elsevier. https://doi.org/10.1016/j.neuron.2018.02.024
chicago: Hu, Hua, Fabian Roth, David H Vandael, and Peter M Jonas. “Complementary
Tuning of Na+ and K+ Channel Gating Underlies Fast and Energy-Efficient Action
Potentials in GABAergic Interneuron Axons.” Neuron. Elsevier, 2018. https://doi.org/10.1016/j.neuron.2018.02.024.
ieee: H. Hu, F. Roth, D. H. Vandael, and P. M. Jonas, “Complementary tuning of Na+
and K+ channel gating underlies fast and energy-efficient action potentials in
GABAergic interneuron axons,” Neuron, vol. 98, no. 1. Elsevier, pp. 156–165,
2018.
ista: Hu H, Roth F, Vandael DH, Jonas PM. 2018. Complementary tuning of Na+ and
K+ channel gating underlies fast and energy-efficient action potentials in GABAergic
interneuron axons. Neuron. 98(1), 156–165.
mla: Hu, Hua, et al. “Complementary Tuning of Na+ and K+ Channel Gating Underlies
Fast and Energy-Efficient Action Potentials in GABAergic Interneuron Axons.” Neuron,
vol. 98, no. 1, Elsevier, 2018, pp. 156–65, doi:10.1016/j.neuron.2018.02.024.
short: H. Hu, F. Roth, D.H. Vandael, P.M. Jonas, Neuron 98 (2018) 156–165.
date_created: 2018-12-11T11:45:48Z
date_published: 2018-04-04T00:00:00Z
date_updated: 2023-09-11T12:45:10Z
day: '04'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1016/j.neuron.2018.02.024
ec_funded: 1
external_id:
isi:
- '000429192100016'
file:
- access_level: open_access
checksum: 76070f3729f9c603e1080d0151aa2b11
content_type: application/pdf
creator: dernst
date_created: 2018-12-17T10:37:50Z
date_updated: 2020-07-14T12:46:03Z
file_id: '5690'
file_name: 2018_Neuron_Hu.pdf
file_size: 3180444
relation: main_file
file_date_updated: 2020-07-14T12:46:03Z
has_accepted_license: '1'
intvolume: ' 98'
isi: 1
issue: '1'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
page: 156 - 165
project:
- _id: 25C0F108-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '268548'
name: Nanophysiology of fast-spiking, parvalbumin-expressing GABAergic interneurons
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: 25C26B1E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P24909-B24
name: Mechanisms of transmitter release at GABAergic synapses
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: The Wittgenstein Prize
publication: Neuron
publication_status: published
publisher: Elsevier
publist_id: '7545'
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/a-certain-type-of-neurons-is-more-energy-efficient-than-previously-assumed/
scopus_import: '1'
status: public
title: Complementary tuning of Na+ and K+ channel gating underlies fast and energy-efficient
action potentials in GABAergic interneuron axons
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 98
year: '2018'
...
---
_id: '324'
abstract:
- lang: eng
text: Neuronal networks in the brain consist of two main types of neuron, glutamatergic
principal neurons and GABAergic interneurons. Although these interneurons only
represent 10–20% of the whole population, they mediate feedback and feedforward
inhibition and are involved in the generation of high-frequency network oscillations.
A hallmark functional property of GABAergic interneurons, especially of the parvalbumin‑expressing
(PV+) subtypes, is the speed of signaling at their output synapse across species
and brain regions. Several molecular and subcellular factors may underlie the
submillisecond signaling at GABAergic synapses. Such as the selective use of P/Q
type Ca2+ channels and the tight coupling between Ca2+ channels and Ca2+ sensors
of exocytosis. However, whether the molecular identity of the release sensor contributes
to these signaling properties remains unclear. Besides, these interneurons are
mainly show depression in response to train of stimuli. How could they keep sufficient
release to control the activity of postsynaptic principal neurons during high
network activity, is largely elusive. For my Ph.D. work, we firstly examined the
Ca2+ sensor of exocytosis at the GABAergic basket cell (BC) to Purkinje cell (PC)
synapse in the cerebellum. Immunolabeling suggested that BC terminals selectively
expressed synaptotagmin 2 (Syt2), whereas synaptotagmin 1 (Syt1) was enriched
in excitatory terminals. Genetic elimination of Syt2 reduced action potential-evoked
release to ~10% compared to the wild-type control, identifying Syt2 as the major
Ca2+ sensor at BC‑PC synapses. Differential adenovirus-mediated rescue revealed
Syt2 triggered release with shorter latency and higher temporal precision, and
mediated faster vesicle pool replenishment than Syt1. Furthermore, deletion of
Syt2 severely reduced and delayed disynaptic inhibition following parallel fiber
stimulation. Thus, the selective use of Syt2 as the release sensor at BC–PC synapse
ensures fast feedforward inhibition in cerebellar microcircuits. Additionally,
we tested the function of another synaptotagmin member, Syt7, for inhibitory synaptic
transmission at the BC–PC synapse. Syt7 is thought to be a Ca2+ sensor that mediates
asynchronous transmitter release and facilitation at synapses. However, it is
strongly expressed in fast-spiking, PV+ GABAergic interneurons and the output
synapses of these neurons produce only minimal asynchronous release and show depression
rather than facilitation. How could Syt7, a facilitation sensor, contribute to
the depressed inhibitory synaptic transmission needs to be further investigated
and understood. Our results indicated that at the BC–PC synapse, Syt7 contributes
to asynchronous release, pool replenishment and facilitation. In combination,
these three effects ensure efficient transmitter release during high‑frequency
activity and guarantee frequency independence of inhibition. Taken together, our
results confirmed that Syt2, which has the fastest kinetic properties among all
synaptotagmin members, is mainly used by the inhibitory BC‑PC synapse for synaptic
transmission, contributing to the speed and temporal precision of transmitter
release. Furthermore, we showed that Syt7, another highly expressed synaptotagmin
member in the output synapses of cerebellar BCs, is used for ensuring efficient
inhibitor synaptic transmission during high activity.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Chong
full_name: Chen, Chong
id: 3DFD581A-F248-11E8-B48F-1D18A9856A87
last_name: Chen
citation:
ama: Chen C. Synaptotagmins ensure speed and efficiency of inhibitory neurotransmitter
release. 2018. doi:10.15479/AT:ISTA:th_997
apa: Chen, C. (2018). Synaptotagmins ensure speed and efficiency of inhibitory
neurotransmitter release. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:th_997
chicago: Chen, Chong. “Synaptotagmins Ensure Speed and Efficiency of Inhibitory
Neurotransmitter Release.” Institute of Science and Technology Austria, 2018.
https://doi.org/10.15479/AT:ISTA:th_997.
ieee: C. Chen, “Synaptotagmins ensure speed and efficiency of inhibitory neurotransmitter
release,” Institute of Science and Technology Austria, 2018.
ista: Chen C. 2018. Synaptotagmins ensure speed and efficiency of inhibitory neurotransmitter
release. Institute of Science and Technology Austria.
mla: Chen, Chong. Synaptotagmins Ensure Speed and Efficiency of Inhibitory Neurotransmitter
Release. Institute of Science and Technology Austria, 2018, doi:10.15479/AT:ISTA:th_997.
short: C. Chen, Synaptotagmins Ensure Speed and Efficiency of Inhibitory Neurotransmitter
Release, Institute of Science and Technology Austria, 2018.
date_created: 2018-12-11T11:45:49Z
date_published: 2018-03-01T00:00:00Z
date_updated: 2023-09-27T12:26:03Z
day: '01'
ddc:
- '571'
degree_awarded: PhD
department:
- _id: PeJo
doi: 10.15479/AT:ISTA:th_997
file:
- access_level: open_access
checksum: 8e163ae9e927401b9fa7c1b3e6a3631a
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:13:58Z
date_updated: 2020-07-14T12:46:04Z
file_id: '5046'
file_name: IST-2018-997-v1+1_Thesis_chong_a.pdf
file_size: 8719458
relation: main_file
- access_level: closed
checksum: f7d7260029a5fbb5c982db61328ade52
content_type: application/octet-stream
creator: dernst
date_created: 2019-04-05T09:25:26Z
date_updated: 2020-07-14T12:46:04Z
file_id: '6221'
file_name: 2018_Thesis_chong_source.pages
file_size: 47841940
relation: source_file
file_date_updated: 2020-07-14T12:46:04Z
has_accepted_license: '1'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
page: '110'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '7541'
pubrep_id: '997'
related_material:
record:
- id: '1117'
relation: part_of_dissertation
status: public
- id: '749'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
title: Synaptotagmins ensure speed and efficiency of inhibitory neurotransmitter release
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2018'
...
---
_id: '21'
abstract:
- lang: eng
text: Parvalbumin-positive (PV+) GABAergic interneurons in hippocampal microcircuits
are thought to play a key role in several higher network functions, such as feedforward
and feedback inhibition, network oscillations, and pattern separation. Fast lateral
inhibition mediated by GABAergic interneurons may implement a winner-takes-all
mechanism in the hippocampal input layer. However, it is not clear whether the
functional connectivity rules of granule cells (GCs) and interneurons in the dentate
gyrus are consistent with such a mechanism. Using simultaneous patch-clamp recordings
from up to seven GCs and up to four PV+ interneurons in the dentate gyrus, we
find that connectivity is structured in space, synapse-specific, and enriched
in specific disynaptic motifs. In contrast to the neocortex, lateral inhibition
in the dentate gyrus (in which a GC inhibits neighboring GCs via a PV+ interneuron)
is ~ 10-times more abundant than recurrent inhibition (in which a GC inhibits
itself). Thus, unique connectivity rules may enable the dentate gyrus to perform
specific higher-order computations
acknowledgement: This project received funding from the European Research Council
(ERC) under the European Union’s Horizon 2020 research and innovation programme
(grant agreement No 692692) and the Fond zur Förderung der Wissenschaftlichen Forschung
(Z 312-B27, Wittgenstein award), both to P.J..
article_number: '4605'
article_processing_charge: No
article_type: original
author:
- first_name: 'Claudia '
full_name: 'Espinoza Martinez, Claudia '
id: 31FFEE2E-F248-11E8-B48F-1D18A9856A87
last_name: Espinoza Martinez
orcid: 0000-0003-4710-2082
- first_name: José
full_name: Guzmán, José
id: 30CC5506-F248-11E8-B48F-1D18A9856A87
last_name: Guzmán
orcid: 0000-0003-2209-5242
- first_name: Xiaomin
full_name: Zhang, Xiaomin
id: 423EC9C2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Espinoza Martinez C, Guzmán J, Zhang X, Jonas PM. Parvalbumin+ interneurons
obey unique connectivity rules and establish a powerful lateral-inhibition microcircuit
in dentate gyrus. Nature Communications. 2018;9(1). doi:10.1038/s41467-018-06899-3
apa: Espinoza Martinez, C., Guzmán, J., Zhang, X., & Jonas, P. M. (2018). Parvalbumin+
interneurons obey unique connectivity rules and establish a powerful lateral-inhibition
microcircuit in dentate gyrus. Nature Communications. Nature Publishing
Group. https://doi.org/10.1038/s41467-018-06899-3
chicago: Espinoza Martinez, Claudia , José Guzmán, Xiaomin Zhang, and Peter M Jonas.
“Parvalbumin+ Interneurons Obey Unique Connectivity Rules and Establish a Powerful
Lateral-Inhibition Microcircuit in Dentate Gyrus.” Nature Communications.
Nature Publishing Group, 2018. https://doi.org/10.1038/s41467-018-06899-3.
ieee: C. Espinoza Martinez, J. Guzmán, X. Zhang, and P. M. Jonas, “Parvalbumin+
interneurons obey unique connectivity rules and establish a powerful lateral-inhibition
microcircuit in dentate gyrus,” Nature Communications, vol. 9, no. 1. Nature
Publishing Group, 2018.
ista: Espinoza Martinez C, Guzmán J, Zhang X, Jonas PM. 2018. Parvalbumin+ interneurons
obey unique connectivity rules and establish a powerful lateral-inhibition microcircuit
in dentate gyrus. Nature Communications. 9(1), 4605.
mla: Espinoza Martinez, Claudia, et al. “Parvalbumin+ Interneurons Obey Unique Connectivity
Rules and Establish a Powerful Lateral-Inhibition Microcircuit in Dentate Gyrus.”
Nature Communications, vol. 9, no. 1, 4605, Nature Publishing Group, 2018,
doi:10.1038/s41467-018-06899-3.
short: C. Espinoza Martinez, J. Guzmán, X. Zhang, P.M. Jonas, Nature Communications
9 (2018).
date_created: 2018-12-11T11:44:12Z
date_published: 2018-11-02T00:00:00Z
date_updated: 2024-03-27T23:30:31Z
day: '02'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1038/s41467-018-06899-3
ec_funded: 1
external_id:
isi:
- '000449069700009'
file:
- access_level: open_access
checksum: 9fe2a63bd95a5067d896c087d07998f3
content_type: application/pdf
creator: dernst
date_created: 2018-12-17T15:41:57Z
date_updated: 2020-07-14T12:45:28Z
file_id: '5715'
file_name: 2018_NatureComm_Espinoza.pdf
file_size: 4651930
relation: main_file
file_date_updated: 2020-07-14T12:45:28Z
has_accepted_license: '1'
intvolume: ' 9'
isi: 1
issue: '1'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: The Wittgenstein Prize
publication: Nature Communications
publication_status: published
publisher: Nature Publishing Group
publist_id: '8034'
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/lateral-inhibition-keeps-similar-memories-apart/
record:
- id: '6363'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Parvalbumin+ interneurons obey unique connectivity rules and establish a powerful
lateral-inhibition microcircuit in dentate gyrus
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 9
year: '2018'
...
---
_id: '630'
abstract:
- lang: eng
text: 'Background: Standards have become available to share semantically encoded
vital parameters from medical devices, as required for example by personal healthcare
records. Standardised sharing of biosignal data largely remains open. Objectives:
The goal of this work is to explore available biosignal file format and data exchange
standards and profiles, and to conceptualise end-To-end solutions. Methods: The
authors reviewed and discussed available biosignal file format standards with
other members of international standards development organisations (SDOs). Results:
A raw concept for standards based acquisition, storage, archiving and sharing
of biosignals was developed. The GDF format may serve for storing biosignals.
Signals can then be shared using FHIR resources and may be stored on FHIR servers
or in DICOM archives, with DICOM waveforms as one possible format. Conclusion:
Currently a group of international SDOs (e.g. HL7, IHE, DICOM, IEEE) is engaged
in intensive discussions. This discussion extends existing work that already was
adopted by large implementer communities. The concept presented here only reports
the current status of the discussion in Austria. The discussion will continue
internationally, with results to be expected over the coming years.'
alternative_title:
- Studies in Health Technology and Informatics
author:
- first_name: Stefan
full_name: Sauermann, Stefan
last_name: Sauermann
- first_name: Veronika
full_name: David, Veronika
last_name: David
- first_name: Alois
full_name: Schlögl, Alois
id: 45BF87EE-F248-11E8-B48F-1D18A9856A87
last_name: Schlögl
orcid: 0000-0002-5621-8100
- first_name: Reinhard
full_name: Egelkraut, Reinhard
last_name: Egelkraut
- first_name: Matthias
full_name: Frohner, Matthias
last_name: Frohner
- first_name: Birgit
full_name: Pohn, Birgit
last_name: Pohn
- first_name: Philipp
full_name: Urbauer, Philipp
last_name: Urbauer
- first_name: Alexander
full_name: Mense, Alexander
last_name: Mense
citation:
ama: 'Sauermann S, David V, Schlögl A, et al. Biosignals standards and FHIR: The
way to go. In: Vol 236. IOS Press; 2017:356-362. doi:10.3233/978-1-61499-759-7-356'
apa: 'Sauermann, S., David, V., Schlögl, A., Egelkraut, R., Frohner, M., Pohn, B.,
… Mense, A. (2017). Biosignals standards and FHIR: The way to go (Vol. 236, pp.
356–362). Presented at the eHealth: Health Informatics Meets eHealth, Vienna,
Austria: IOS Press. https://doi.org/10.3233/978-1-61499-759-7-356'
chicago: 'Sauermann, Stefan, Veronika David, Alois Schlögl, Reinhard Egelkraut,
Matthias Frohner, Birgit Pohn, Philipp Urbauer, and Alexander Mense. “Biosignals
Standards and FHIR: The Way to Go,” 236:356–62. IOS Press, 2017. https://doi.org/10.3233/978-1-61499-759-7-356.'
ieee: 'S. Sauermann et al., “Biosignals standards and FHIR: The way to go,”
presented at the eHealth: Health Informatics Meets eHealth, Vienna, Austria, 2017,
vol. 236, pp. 356–362.'
ista: 'Sauermann S, David V, Schlögl A, Egelkraut R, Frohner M, Pohn B, Urbauer
P, Mense A. 2017. Biosignals standards and FHIR: The way to go. eHealth: Health
Informatics Meets eHealth, Studies in Health Technology and Informatics, vol.
236, 356–362.'
mla: 'Sauermann, Stefan, et al. Biosignals Standards and FHIR: The Way to Go.
Vol. 236, IOS Press, 2017, pp. 356–62, doi:10.3233/978-1-61499-759-7-356.'
short: S. Sauermann, V. David, A. Schlögl, R. Egelkraut, M. Frohner, B. Pohn, P.
Urbauer, A. Mense, in:, IOS Press, 2017, pp. 356–362.
conference:
end_date: 2017-05-24
location: Vienna, Austria
name: 'eHealth: Health Informatics Meets eHealth'
start_date: 2017-05-23
date_created: 2018-12-11T11:47:36Z
date_published: 2017-01-01T00:00:00Z
date_updated: 2021-01-12T08:06:59Z
day: '01'
ddc:
- '005'
department:
- _id: ScienComp
- _id: PeJo
doi: 10.3233/978-1-61499-759-7-356
file:
- access_level: open_access
checksum: 1254dcc5b04a996d97fad9a726b42727
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:11:56Z
date_updated: 2020-07-14T12:47:27Z
file_id: '4913'
file_name: IST-2017-906-v1+1_SHTI236-0356.pdf
file_size: 443635
relation: main_file
file_date_updated: 2020-07-14T12:47:27Z
has_accepted_license: '1'
intvolume: ' 236'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc/4.0/
month: '01'
oa: 1
oa_version: Published Version
page: 356 - 362
publication_identifier:
isbn:
- 978-161499758-0
publication_status: published
publisher: IOS Press
publist_id: '7164'
pubrep_id: '906'
quality_controlled: '1'
scopus_import: 1
status: public
title: 'Biosignals standards and FHIR: The way to go'
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: conference
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 236
year: '2017'
...
---
_id: '706'
abstract:
- lang: eng
text: A hippocampal mossy fiber synapse has a complex structure and is implicated
in learning and memory. In this synapse, the mossy fiber boutons attach to the
dendritic shaft by puncta adherentia junctions and wrap around a multiply-branched
spine, forming synaptic junctions. We have recently shown using transmission electron
microscopy, immunoelectron microscopy and serial block face-scanning electron
microscopy that atypical puncta adherentia junctions are formed in the afadin-deficient
mossy fiber synapse and that the complexity of postsynaptic spines and mossy fiber
boutons, the number of spine heads, the area of postsynaptic densities and the
density of synaptic vesicles docked to active zones are decreased in the afadin-deficient
synapse. We investigated here the roles of afadin in the functional differentiations
of the mossy fiber synapse using the afadin-deficient mice. The electrophysiological
studies showed that both the release probability of glutamate and the postsynaptic
responsiveness to glutamate were markedly reduced, but not completely lost, in
the afadin-deficient mossy fiber synapse, whereas neither long-term potentiation
nor long-term depression was affected. These results indicate that afadin plays
roles in the functional differentiations of the presynapse and the postsynapse
of the hippocampal mossy fiber synapse.
author:
- first_name: Xiaoqi
full_name: Geng, Xiaoqi
id: 3395256A-F248-11E8-B48F-1D18A9856A87
last_name: Geng
- first_name: Tomohiko
full_name: Maruo, Tomohiko
last_name: Maruo
- first_name: Kenji
full_name: Mandai, Kenji
last_name: Mandai
- first_name: Irwan
full_name: Supriyanto, Irwan
last_name: Supriyanto
- first_name: Muneaki
full_name: Miyata, Muneaki
last_name: Miyata
- first_name: Shotaro
full_name: Sakakibara, Shotaro
last_name: Sakakibara
- first_name: Akira
full_name: Mizoguchi, Akira
last_name: Mizoguchi
- first_name: Yoshimi
full_name: Takai, Yoshimi
last_name: Takai
- first_name: Masahiro
full_name: Mori, Masahiro
last_name: Mori
citation:
ama: Geng X, Maruo T, Mandai K, et al. Roles of afadin in functional differentiations
of hippocampal mossy fiber synapse. Genes to Cells. 2017;22(8):715-722.
doi:10.1111/gtc.12508
apa: Geng, X., Maruo, T., Mandai, K., Supriyanto, I., Miyata, M., Sakakibara, S.,
… Mori, M. (2017). Roles of afadin in functional differentiations of hippocampal
mossy fiber synapse. Genes to Cells. Wiley-Blackwell. https://doi.org/10.1111/gtc.12508
chicago: Geng, Xiaoqi, Tomohiko Maruo, Kenji Mandai, Irwan Supriyanto, Muneaki Miyata,
Shotaro Sakakibara, Akira Mizoguchi, Yoshimi Takai, and Masahiro Mori. “Roles
of Afadin in Functional Differentiations of Hippocampal Mossy Fiber Synapse.”
Genes to Cells. Wiley-Blackwell, 2017. https://doi.org/10.1111/gtc.12508.
ieee: X. Geng et al., “Roles of afadin in functional differentiations of
hippocampal mossy fiber synapse,” Genes to Cells, vol. 22, no. 8. Wiley-Blackwell,
pp. 715–722, 2017.
ista: Geng X, Maruo T, Mandai K, Supriyanto I, Miyata M, Sakakibara S, Mizoguchi
A, Takai Y, Mori M. 2017. Roles of afadin in functional differentiations of hippocampal
mossy fiber synapse. Genes to Cells. 22(8), 715–722.
mla: Geng, Xiaoqi, et al. “Roles of Afadin in Functional Differentiations of Hippocampal
Mossy Fiber Synapse.” Genes to Cells, vol. 22, no. 8, Wiley-Blackwell,
2017, pp. 715–22, doi:10.1111/gtc.12508.
short: X. Geng, T. Maruo, K. Mandai, I. Supriyanto, M. Miyata, S. Sakakibara, A.
Mizoguchi, Y. Takai, M. Mori, Genes to Cells 22 (2017) 715–722.
date_created: 2018-12-11T11:48:02Z
date_published: 2017-08-01T00:00:00Z
date_updated: 2021-01-12T08:11:37Z
day: '01'
department:
- _id: PeJo
doi: 10.1111/gtc.12508
intvolume: ' 22'
issue: '8'
language:
- iso: eng
month: '08'
oa_version: None
page: 715 - 722
publication: Genes to Cells
publication_identifier:
issn:
- '13569597'
publication_status: published
publisher: Wiley-Blackwell
publist_id: '6987'
quality_controlled: '1'
scopus_import: 1
status: public
title: Roles of afadin in functional differentiations of hippocampal mossy fiber synapse
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 22
year: '2017'
...
---
_id: '1118'
abstract:
- lang: eng
text: Sharp wave-ripple (SWR) oscillations play a key role in memory consolidation
during non-rapid eye movement sleep, immobility, and consummatory behavior. However,
whether temporally modulated synaptic excitation or inhibition underlies the ripples
is controversial. To address this question, we performed simultaneous recordings
of excitatory and inhibitory postsynaptic currents (EPSCs and IPSCs) and local
field potentials (LFPs) in the CA1 region of awake mice in vivo. During SWRs,
inhibition dominated over excitation, with a peak conductance ratio of 4.1 ± 0.5.
Furthermore, the amplitude of SWR-associated IPSCs was positively correlated with
SWR magnitude, whereas that of EPSCs was not. Finally, phase analysis indicated
that IPSCs were phase-locked to individual ripple cycles, whereas EPSCs were uniformly
distributed in phase space. Optogenetic inhibition indicated that PV+ interneurons
provided a major contribution to SWR-associated IPSCs. Thus, phasic inhibition,
but not excitation, shapes SWR oscillations in the hippocampal CA1 region in vivo.
acknowledged_ssus:
- _id: M-Shop
- _id: ScienComp
- _id: PreCl
article_processing_charge: No
author:
- first_name: Jian
full_name: Gan, Jian
id: 3614E438-F248-11E8-B48F-1D18A9856A87
last_name: Gan
- first_name: Shih-Ming
full_name: Weng, Shih-Ming
id: 2F9C5AC8-F248-11E8-B48F-1D18A9856A87
last_name: Weng
- first_name: Alejandro
full_name: Pernia-Andrade, Alejandro
id: 36963E98-F248-11E8-B48F-1D18A9856A87
last_name: Pernia-Andrade
- first_name: Jozsef L
full_name: Csicsvari, Jozsef L
id: 3FA14672-F248-11E8-B48F-1D18A9856A87
last_name: Csicsvari
orcid: 0000-0002-5193-4036
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Gan J, Weng S-M, Pernia-Andrade A, Csicsvari JL, Jonas PM. Phase-locked inhibition,
but not excitation, underlies hippocampal ripple oscillations in awake mice in
vivo. Neuron. 2017;93(2):308-314. doi:10.1016/j.neuron.2016.12.018
apa: Gan, J., Weng, S.-M., Pernia-Andrade, A., Csicsvari, J. L., & Jonas, P.
M. (2017). Phase-locked inhibition, but not excitation, underlies hippocampal
ripple oscillations in awake mice in vivo. Neuron. Elsevier. https://doi.org/10.1016/j.neuron.2016.12.018
chicago: Gan, Jian, Shih-Ming Weng, Alejandro Pernia-Andrade, Jozsef L Csicsvari,
and Peter M Jonas. “Phase-Locked Inhibition, but Not Excitation, Underlies Hippocampal
Ripple Oscillations in Awake Mice in Vivo.” Neuron. Elsevier, 2017. https://doi.org/10.1016/j.neuron.2016.12.018.
ieee: J. Gan, S.-M. Weng, A. Pernia-Andrade, J. L. Csicsvari, and P. M. Jonas, “Phase-locked
inhibition, but not excitation, underlies hippocampal ripple oscillations in awake
mice in vivo,” Neuron, vol. 93, no. 2. Elsevier, pp. 308–314, 2017.
ista: Gan J, Weng S-M, Pernia-Andrade A, Csicsvari JL, Jonas PM. 2017. Phase-locked
inhibition, but not excitation, underlies hippocampal ripple oscillations in awake
mice in vivo. Neuron. 93(2), 308–314.
mla: Gan, Jian, et al. “Phase-Locked Inhibition, but Not Excitation, Underlies Hippocampal
Ripple Oscillations in Awake Mice in Vivo.” Neuron, vol. 93, no. 2, Elsevier,
2017, pp. 308–14, doi:10.1016/j.neuron.2016.12.018.
short: J. Gan, S.-M. Weng, A. Pernia-Andrade, J.L. Csicsvari, P.M. Jonas, Neuron
93 (2017) 308–314.
date_created: 2018-12-11T11:50:15Z
date_published: 2017-01-18T00:00:00Z
date_updated: 2023-09-20T11:31:48Z
day: '18'
ddc:
- '571'
department:
- _id: PeJo
- _id: JoCs
doi: 10.1016/j.neuron.2016.12.018
ec_funded: 1
external_id:
isi:
- '000396428200010'
file:
- access_level: open_access
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:08:56Z
date_updated: 2018-12-12T10:08:56Z
file_id: '4719'
file_name: IST-2017-752-v1+1_1-s2.0-S0896627316309606-main.pdf
file_size: 2738950
relation: main_file
file_date_updated: 2018-12-12T10:08:56Z
has_accepted_license: '1'
intvolume: ' 93'
isi: 1
issue: '2'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: 308 - 314
project:
- _id: 25C26B1E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P24909-B24
name: Mechanisms of transmitter release at GABAergic synapses
- _id: 25C0F108-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '268548'
name: Nanophysiology of fast-spiking, parvalbumin-expressing GABAergic interneurons
publication: Neuron
publication_status: published
publisher: Elsevier
publist_id: '6244'
pubrep_id: '752'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Phase-locked inhibition, but not excitation, underlies hippocampal ripple oscillations
in awake mice in vivo
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 93
year: '2017'
...
---
_id: '1117'
abstract:
- lang: eng
text: 'GABAergic synapses in brain circuits generate inhibitory output signals with
submillisecond latency and temporal precision. Whether the molecular identity
of the release sensor contributes to these signaling properties remains unclear.
Here, we examined the Ca^2+ sensor of exocytosis at GABAergic basket cell (BC)
to Purkinje cell (PC) synapses in cerebellum. Immunolabeling suggested that BC
terminals selectively expressed synaptotagmin 2 (Syt2), whereas synaptotagmin
1 (Syt1) was enriched in excitatory terminals. Genetic elimination of Syt2 reduced
action potential-evoked release to ∼10%, identifying Syt2 as the major Ca^2+ sensor
at BC-PC synapses. Differential adenovirus-mediated rescue revealed that Syt2
triggered release with shorter latency and higher temporal precision and mediated
faster vesicle pool replenishment than Syt1. Furthermore, deletion of Syt2 severely
reduced and delayed disynaptic inhibition following parallel fiber stimulation.
Thus, the selective use of Syt2 as release sensor at BC-PC synapses ensures fast
and efficient feedforward inhibition in cerebellar microcircuits. #bioimagingfacility-author'
acknowledged_ssus:
- _id: Bio
- _id: PreCl
article_processing_charge: No
author:
- first_name: Chong
full_name: Chen, Chong
id: 3DFD581A-F248-11E8-B48F-1D18A9856A87
last_name: Chen
- first_name: Itaru
full_name: Arai, Itaru
id: 32A73F6C-F248-11E8-B48F-1D18A9856A87
last_name: Arai
- first_name: Rachel
full_name: Satterield, Rachel
last_name: Satterield
- first_name: Samuel
full_name: Young, Samuel
last_name: Young
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Chen C, Arai itaru, Satterield R, Young S, Jonas PM. Synaptotagmin 2 is the
fast Ca2+ sensor at a central inhibitory synapse. Cell Reports. 2017;18(3):723-736.
doi:10.1016/j.celrep.2016.12.067
apa: Chen, C., Arai, itaru, Satterield, R., Young, S., & Jonas, P. M. (2017).
Synaptotagmin 2 is the fast Ca2+ sensor at a central inhibitory synapse. Cell
Reports. Cell Press. https://doi.org/10.1016/j.celrep.2016.12.067
chicago: Chen, Chong, itaru Arai, Rachel Satterield, Samuel Young, and Peter M Jonas.
“Synaptotagmin 2 Is the Fast Ca2+ Sensor at a Central Inhibitory Synapse.” Cell
Reports. Cell Press, 2017. https://doi.org/10.1016/j.celrep.2016.12.067.
ieee: C. Chen, itaru Arai, R. Satterield, S. Young, and P. M. Jonas, “Synaptotagmin
2 is the fast Ca2+ sensor at a central inhibitory synapse,” Cell Reports,
vol. 18, no. 3. Cell Press, pp. 723–736, 2017.
ista: Chen C, Arai itaru, Satterield R, Young S, Jonas PM. 2017. Synaptotagmin
2 is the fast Ca2+ sensor at a central inhibitory synapse. Cell Reports. 18(3),
723–736.
mla: Chen, Chong, et al. “Synaptotagmin 2 Is the Fast Ca2+ Sensor at a Central Inhibitory
Synapse.” Cell Reports, vol. 18, no. 3, Cell Press, 2017, pp. 723–36, doi:10.1016/j.celrep.2016.12.067.
short: C. Chen, itaru Arai, R. Satterield, S. Young, P.M. Jonas, Cell Reports 18
(2017) 723–736.
date_created: 2018-12-11T11:50:14Z
date_published: 2017-01-17T00:00:00Z
date_updated: 2023-09-20T11:32:15Z
day: '17'
ddc:
- '571'
department:
- _id: PeJo
doi: 10.1016/j.celrep.2016.12.067
ec_funded: 1
external_id:
isi:
- '000396470600013'
file:
- access_level: open_access
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:16:09Z
date_updated: 2018-12-12T10:16:09Z
file_id: '5195'
file_name: IST-2017-751-v1+1_1-s2.0-S2211124716317740-main.pdf
file_size: 4427591
relation: main_file
file_date_updated: 2018-12-12T10:16:09Z
has_accepted_license: '1'
intvolume: ' 18'
isi: 1
issue: '3'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: 723 - 736
project:
- _id: 25C26B1E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P24909-B24
name: Mechanisms of transmitter release at GABAergic synapses
- _id: 25C0F108-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '268548'
name: Nanophysiology of fast-spiking, parvalbumin-expressing GABAergic interneurons
publication: Cell Reports
publication_identifier:
issn:
- '22111247'
publication_status: published
publisher: Cell Press
publist_id: '6245'
pubrep_id: '751'
quality_controlled: '1'
related_material:
record:
- id: '324'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Synaptotagmin 2 is the fast Ca2+ sensor at a central inhibitory synapse
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 18
year: '2017'
...
---
_id: '991'
abstract:
- lang: eng
text: Synaptotagmin 7 (Syt7) was originally identified as a slow Ca2+ sensor for
lysosome fusion, but its function at fast synapses is controversial. The paper
by Luo and Südhof (2017) in this issue of Neuron shows that at the calyx of Held
in the auditory brainstem Syt7 triggers asynchronous release during stimulus trains,
resulting in reliable and temporally precise high-frequency transmission. Thus,
a slow Ca2+ sensor contributes to the fast signaling properties of the calyx synapse.
article_processing_charge: No
author:
- first_name: Chong
full_name: Chen, Chong
id: 3DFD581A-F248-11E8-B48F-1D18A9856A87
last_name: Chen
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: 'Chen C, Jonas PM. Synaptotagmins: That’s why so many. Neuron. 2017;94(4):694-696.
doi:10.1016/j.neuron.2017.05.011'
apa: 'Chen, C., & Jonas, P. M. (2017). Synaptotagmins: That’s why so many. Neuron.
Elsevier. https://doi.org/10.1016/j.neuron.2017.05.011'
chicago: 'Chen, Chong, and Peter M Jonas. “Synaptotagmins: That’s Why so Many.”
Neuron. Elsevier, 2017. https://doi.org/10.1016/j.neuron.2017.05.011.'
ieee: 'C. Chen and P. M. Jonas, “Synaptotagmins: That’s why so many,” Neuron,
vol. 94, no. 4. Elsevier, pp. 694–696, 2017.'
ista: 'Chen C, Jonas PM. 2017. Synaptotagmins: That’s why so many. Neuron. 94(4),
694–696.'
mla: 'Chen, Chong, and Peter M. Jonas. “Synaptotagmins: That’s Why so Many.” Neuron,
vol. 94, no. 4, Elsevier, 2017, pp. 694–96, doi:10.1016/j.neuron.2017.05.011.'
short: C. Chen, P.M. Jonas, Neuron 94 (2017) 694–696.
date_created: 2018-12-11T11:49:34Z
date_published: 2017-05-17T00:00:00Z
date_updated: 2023-09-22T09:54:37Z
day: '17'
department:
- _id: PeJo
doi: 10.1016/j.neuron.2017.05.011
external_id:
isi:
- '000401415100002'
intvolume: ' 94'
isi: 1
issue: '4'
language:
- iso: eng
month: '05'
oa_version: None
page: 694 - 696
publication: Neuron
publication_identifier:
issn:
- '08966273'
publication_status: published
publisher: Elsevier
publist_id: '6408'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Synaptotagmins: That’s why so many'
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 94
year: '2017'
...
---
_id: '800'
abstract:
- lang: eng
text: Gamma oscillations (30–150 Hz) in neuronal networks are associated with the
processing and recall of information. We measured local field potentials in the
dentate gyrus of freely moving mice and found that gamma activity occurs in bursts,
which are highly heterogeneous in their spatial extensions, ranging from focal
to global coherent events. Synaptic communication among perisomatic-inhibitory
interneurons (PIIs) is thought to play an important role in the generation of
hippocampal gamma patterns. However, how neuronal circuits can generate synchronous
oscillations at different spatial scales is unknown. We analyzed paired recordings
in dentate gyrus slices and show that synaptic signaling at interneuron-interneuron
synapses is distance dependent. Synaptic strength declines whereas the duration
of inhibitory signals increases with axonal distance among interconnected PIIs.
Using neuronal network modeling, we show that distance-dependent inhibition generates
multiple highly synchronous focal gamma bursts allowing the network to process
complex inputs in parallel in flexibly organized neuronal centers.
article_number: '758'
article_processing_charge: No
author:
- first_name: Michael
full_name: Strüber, Michael
last_name: Strüber
- first_name: Jonas
full_name: Sauer, Jonas
last_name: Sauer
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: Marlene
full_name: Bartos, Marlene
last_name: Bartos
citation:
ama: Strüber M, Sauer J, Jonas PM, Bartos M. Distance-dependent inhibition facilitates
focality of gamma oscillations in the dentate gyrus. Nature Communications.
2017;8(1). doi:10.1038/s41467-017-00936-3
apa: Strüber, M., Sauer, J., Jonas, P. M., & Bartos, M. (2017). Distance-dependent
inhibition facilitates focality of gamma oscillations in the dentate gyrus. Nature
Communications. Nature Publishing Group. https://doi.org/10.1038/s41467-017-00936-3
chicago: Strüber, Michael, Jonas Sauer, Peter M Jonas, and Marlene Bartos. “Distance-Dependent
Inhibition Facilitates Focality of Gamma Oscillations in the Dentate Gyrus.” Nature
Communications. Nature Publishing Group, 2017. https://doi.org/10.1038/s41467-017-00936-3.
ieee: M. Strüber, J. Sauer, P. M. Jonas, and M. Bartos, “Distance-dependent inhibition
facilitates focality of gamma oscillations in the dentate gyrus,” Nature Communications,
vol. 8, no. 1. Nature Publishing Group, 2017.
ista: Strüber M, Sauer J, Jonas PM, Bartos M. 2017. Distance-dependent inhibition
facilitates focality of gamma oscillations in the dentate gyrus. Nature Communications.
8(1), 758.
mla: Strüber, Michael, et al. “Distance-Dependent Inhibition Facilitates Focality
of Gamma Oscillations in the Dentate Gyrus.” Nature Communications, vol.
8, no. 1, 758, Nature Publishing Group, 2017, doi:10.1038/s41467-017-00936-3.
short: M. Strüber, J. Sauer, P.M. Jonas, M. Bartos, Nature Communications 8 (2017).
date_created: 2018-12-11T11:48:34Z
date_published: 2017-10-02T00:00:00Z
date_updated: 2023-09-27T10:59:41Z
day: '02'
ddc:
- '571'
department:
- _id: PeJo
doi: 10.1038/s41467-017-00936-3
ec_funded: 1
external_id:
isi:
- '000412053100004'
file:
- access_level: open_access
checksum: 7e2c7621afd5f802338e92e8619f024d
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:15:17Z
date_updated: 2020-07-14T12:48:07Z
file_id: '5135'
file_name: IST-2017-914-v1+1_s41467-017-00936-3.pdf
file_size: 4261832
relation: main_file
file_date_updated: 2020-07-14T12:48:07Z
has_accepted_license: '1'
intvolume: ' 8'
isi: 1
issue: '1'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
project:
- _id: 25C0F108-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '268548'
name: Nanophysiology of fast-spiking, parvalbumin-expressing GABAergic interneurons
publication: Nature Communications
publication_identifier:
issn:
- '20411723'
publication_status: published
publisher: Nature Publishing Group
publist_id: '6853'
pubrep_id: '914'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Distance-dependent inhibition facilitates focality of gamma oscillations in
the dentate gyrus
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 8
year: '2017'
...
---
_id: '749'
abstract:
- lang: eng
text: 'Synaptotagmin 7 (Syt7) is thought to be a Ca2+ sensor that mediates asynchronous
transmitter release and facilitation at synapses. However, Syt7 is strongly expressed
in fast-spiking, parvalbumin-expressing GABAergic interneurons, and the output
synapses of these neurons produce only minimal asynchronous release and show depression
rather than facilitation. To resolve this apparent contradiction, we examined
the effects of genetic elimination of Syt7 on synaptic transmission at the GABAergic
basket cell (BC)-Purkinje cell (PC) synapse in cerebellum. Our results indicate
that at the BC-PC synapse, Syt7 contributes to asynchronous release, pool replenishment,
and facilitation. In combination, these three effects ensure efficient transmitter
release during high-frequency activity and guarantee frequency independence of
inhibition. Our results identify a distinct function of Syt7: ensuring the efficiency
of high-frequency inhibitory synaptic transmission'
acknowledged_ssus:
- _id: PreCl
article_processing_charge: No
author:
- first_name: Chong
full_name: Chen, Chong
id: 3DFD581A-F248-11E8-B48F-1D18A9856A87
last_name: Chen
- first_name: Rachel
full_name: Satterfield, Rachel
last_name: Satterfield
- first_name: Samuel
full_name: Young, Samuel
last_name: Young
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Chen C, Satterfield R, Young S, Jonas PM. Triple function of Synaptotagmin
7 ensures efficiency of high-frequency transmission at central GABAergic synapses.
Cell Reports. 2017;21(8):2082-2089. doi:10.1016/j.celrep.2017.10.122
apa: Chen, C., Satterfield, R., Young, S., & Jonas, P. M. (2017). Triple function
of Synaptotagmin 7 ensures efficiency of high-frequency transmission at central
GABAergic synapses. Cell Reports. Cell Press. https://doi.org/10.1016/j.celrep.2017.10.122
chicago: Chen, Chong, Rachel Satterfield, Samuel Young, and Peter M Jonas. “Triple
Function of Synaptotagmin 7 Ensures Efficiency of High-Frequency Transmission
at Central GABAergic Synapses.” Cell Reports. Cell Press, 2017. https://doi.org/10.1016/j.celrep.2017.10.122.
ieee: C. Chen, R. Satterfield, S. Young, and P. M. Jonas, “Triple function of Synaptotagmin
7 ensures efficiency of high-frequency transmission at central GABAergic synapses,”
Cell Reports, vol. 21, no. 8. Cell Press, pp. 2082–2089, 2017.
ista: Chen C, Satterfield R, Young S, Jonas PM. 2017. Triple function of Synaptotagmin
7 ensures efficiency of high-frequency transmission at central GABAergic synapses.
Cell Reports. 21(8), 2082–2089.
mla: Chen, Chong, et al. “Triple Function of Synaptotagmin 7 Ensures Efficiency
of High-Frequency Transmission at Central GABAergic Synapses.” Cell Reports,
vol. 21, no. 8, Cell Press, 2017, pp. 2082–89, doi:10.1016/j.celrep.2017.10.122.
short: C. Chen, R. Satterfield, S. Young, P.M. Jonas, Cell Reports 21 (2017) 2082–2089.
date_created: 2018-12-11T11:48:18Z
date_published: 2017-11-21T00:00:00Z
date_updated: 2023-09-27T12:26:04Z
day: '21'
ddc:
- '570'
- '571'
department:
- _id: PeJo
doi: 10.1016/j.celrep.2017.10.122
ec_funded: 1
external_id:
isi:
- '000416216700007'
file:
- access_level: open_access
checksum: a6afa3764909bf6edafa07982d8e1cee
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:09:14Z
date_updated: 2020-07-14T12:47:59Z
file_id: '4737'
file_name: IST-2017-874-v1+1_PIIS2211124717316029.pdf
file_size: 2759195
relation: main_file
file_date_updated: 2020-07-14T12:47:59Z
has_accepted_license: '1'
intvolume: ' 21'
isi: 1
issue: '8'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
page: 2082 - 2089
project:
- _id: 25C26B1E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P24909-B24
name: Mechanisms of transmitter release at GABAergic synapses
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glumatergic synapse
publication: Cell Reports
publication_identifier:
issn:
- '22111247'
publication_status: published
publisher: Cell Press
publist_id: '6907'
pubrep_id: '874'
quality_controlled: '1'
related_material:
record:
- id: '324'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Triple function of Synaptotagmin 7 ensures efficiency of high-frequency transmission
at central GABAergic synapses
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 21
year: '2017'
...
---
_id: '1142'
abstract:
- lang: eng
text: Hemolysis drives susceptibility to bacterial infections and predicts poor
outcome from sepsis. These detrimental effects are commonly considered to be a
consequence of heme-iron serving as a nutrient for bacteria. We employed a Gram-negative
sepsis model and found that elevated heme levels impaired the control of bacterial
proliferation independently of heme-iron acquisition by pathogens. Heme strongly
inhibited phagocytosis and the migration of human and mouse phagocytes by disrupting
actin cytoskeletal dynamics via activation of the GTP-binding Rho family protein
Cdc42 by the guanine nucleotide exchange factor DOCK8. A chemical screening approach
revealed that quinine effectively prevented heme effects on the cytoskeleton,
restored phagocytosis and improved survival in sepsis. These mechanistic insights
provide potential therapeutic targets for patients with sepsis or hemolytic disorders.
acknowledgement: 'Y. Fukui (Medical Institute of Bioregulation, Kyushu University)
and J. Stein (Theodor Kocher Institute, University of Bern) are acknowledged for
providing the DOCK8 deficient bone marrow. and H. Häcker (St. Judes Children''s
Research Hospital) for providing the ERHBD-HoxB8-encoding retroviral construct.
pSpCas9(BB)-2a-Puro (PX459) was a gift from F. Zhang (Massachusetts Institute of
Technology) (Addgene plasmid # 48139) and pGRG36 was a gift from N. Craig (Johns
Hopkins University School of Medicine) (Addgene plasmid # 16666). LifeAct-GFP-encoding
retrovirus was kindly provided by A. Leithner (Institute of Science and Technology
Austria). pSIM8 and TKC E. coli were gifts from D.L. Court (Center for Cancer Research,
National Cancer Institute). We acknowledge M. Gröger and S. Rauscher for excellent
technical support (Core imaging facility, Medical University of Vienna). We thank
D.P. Barlow and L.R. Cheever for critical reading of the manuscript. This work was
supported by the Austrian Academy of Sciences, the Science Fund of the Austrian
National Bank (14107) and the Austrian Science Fund FWF (I1620-B22) in the Infect-ERA
framework (to S.Knapp).'
author:
- first_name: Rui
full_name: Martins, Rui
last_name: Martins
- first_name: Julia
full_name: Maier, Julia
last_name: Maier
- first_name: Anna
full_name: Gorki, Anna
last_name: Gorki
- first_name: Kilian
full_name: Huber, Kilian
last_name: Huber
- first_name: Omar
full_name: Sharif, Omar
last_name: Sharif
- first_name: Philipp
full_name: Starkl, Philipp
last_name: Starkl
- first_name: Simona
full_name: Saluzzo, Simona
last_name: Saluzzo
- first_name: Federica
full_name: Quattrone, Federica
last_name: Quattrone
- first_name: Riem
full_name: Gawish, Riem
last_name: Gawish
- first_name: Karin
full_name: Lakovits, Karin
last_name: Lakovits
- first_name: Michael
full_name: Aichinger, Michael
last_name: Aichinger
- first_name: Branka
full_name: Radic Sarikas, Branka
last_name: Radic Sarikas
- first_name: Charles
full_name: Lardeau, Charles
last_name: Lardeau
- first_name: Anastasiya
full_name: Hladik, Anastasiya
last_name: Hladik
- first_name: Ana
full_name: Korosec, Ana
last_name: Korosec
- first_name: Markus
full_name: Brown, Markus
id: 3DAB9AFC-F248-11E8-B48F-1D18A9856A87
last_name: Brown
- first_name: Kari
full_name: Vaahtomeri, Kari
id: 368EE576-F248-11E8-B48F-1D18A9856A87
last_name: Vaahtomeri
orcid: 0000-0001-7829-3518
- first_name: Michelle
full_name: Duggan, Michelle
id: 2EDEA62C-F248-11E8-B48F-1D18A9856A87
last_name: Duggan
- first_name: Dontscho
full_name: Kerjaschki, Dontscho
last_name: Kerjaschki
- first_name: Harald
full_name: Esterbauer, Harald
last_name: Esterbauer
- first_name: Jacques
full_name: Colinge, Jacques
last_name: Colinge
- first_name: Stephanie
full_name: Eisenbarth, Stephanie
last_name: Eisenbarth
- first_name: Thomas
full_name: Decker, Thomas
last_name: Decker
- first_name: Keiryn
full_name: Bennett, Keiryn
last_name: Bennett
- first_name: Stefan
full_name: Kubicek, Stefan
last_name: Kubicek
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Giulio
full_name: Superti Furga, Giulio
last_name: Superti Furga
- first_name: Sylvia
full_name: Knapp, Sylvia
last_name: Knapp
citation:
ama: Martins R, Maier J, Gorki A, et al. Heme drives hemolysis-induced susceptibility
to infection via disruption of phagocyte functions. Nature Immunology.
2016;17(12):1361-1372. doi:10.1038/ni.3590
apa: Martins, R., Maier, J., Gorki, A., Huber, K., Sharif, O., Starkl, P., … Knapp,
S. (2016). Heme drives hemolysis-induced susceptibility to infection via disruption
of phagocyte functions. Nature Immunology. Nature Publishing Group. https://doi.org/10.1038/ni.3590
chicago: Martins, Rui, Julia Maier, Anna Gorki, Kilian Huber, Omar Sharif, Philipp
Starkl, Simona Saluzzo, et al. “Heme Drives Hemolysis-Induced Susceptibility to
Infection via Disruption of Phagocyte Functions.” Nature Immunology. Nature
Publishing Group, 2016. https://doi.org/10.1038/ni.3590.
ieee: R. Martins et al., “Heme drives hemolysis-induced susceptibility to
infection via disruption of phagocyte functions,” Nature Immunology, vol.
17, no. 12. Nature Publishing Group, pp. 1361–1372, 2016.
ista: Martins R, Maier J, Gorki A, Huber K, Sharif O, Starkl P, Saluzzo S, Quattrone
F, Gawish R, Lakovits K, Aichinger M, Radic Sarikas B, Lardeau C, Hladik A, Korosec
A, Brown M, Vaahtomeri K, Duggan M, Kerjaschki D, Esterbauer H, Colinge J, Eisenbarth
S, Decker T, Bennett K, Kubicek S, Sixt MK, Superti Furga G, Knapp S. 2016. Heme
drives hemolysis-induced susceptibility to infection via disruption of phagocyte
functions. Nature Immunology. 17(12), 1361–1372.
mla: Martins, Rui, et al. “Heme Drives Hemolysis-Induced Susceptibility to Infection
via Disruption of Phagocyte Functions.” Nature Immunology, vol. 17, no.
12, Nature Publishing Group, 2016, pp. 1361–72, doi:10.1038/ni.3590.
short: R. Martins, J. Maier, A. Gorki, K. Huber, O. Sharif, P. Starkl, S. Saluzzo,
F. Quattrone, R. Gawish, K. Lakovits, M. Aichinger, B. Radic Sarikas, C. Lardeau,
A. Hladik, A. Korosec, M. Brown, K. Vaahtomeri, M. Duggan, D. Kerjaschki, H. Esterbauer,
J. Colinge, S. Eisenbarth, T. Decker, K. Bennett, S. Kubicek, M.K. Sixt, G. Superti
Furga, S. Knapp, Nature Immunology 17 (2016) 1361–1372.
date_created: 2018-12-11T11:50:22Z
date_published: 2016-12-01T00:00:00Z
date_updated: 2021-01-12T06:48:36Z
day: '01'
department:
- _id: MiSi
- _id: PeJo
doi: 10.1038/ni.3590
intvolume: ' 17'
issue: '12'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://ora.ox.ac.uk/objects/uuid:f53a464e-1e5b-4f08-a7d8-b6749b852b9d
month: '12'
oa: 1
oa_version: Submitted Version
page: 1361 - 1372
publication: Nature Immunology
publication_status: published
publisher: Nature Publishing Group
publist_id: '6216'
quality_controlled: '1'
scopus_import: 1
status: public
title: Heme drives hemolysis-induced susceptibility to infection via disruption of
phagocyte functions
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 17
year: '2016'
...
---
_id: '1323'
abstract:
- lang: eng
text: Mossy fiber synapses on CA3 pyramidal cells are 'conditional detonators' that
reliably discharge postsynaptic targets. The 'conditional' nature implies that
burst activity in dentate gyrus granule cells is required for detonation. Whether
single unitary excitatory postsynaptic potentials (EPSPs) trigger spikes in CA3
neurons remains unknown. Mossy fiber synapses exhibit both pronounced short-term
facilitation and uniquely large post-tetanic potentiation (PTP). We tested whether
PTP could convert mossy fiber synapses from subdetonator into detonator mode,
using a recently developed method to selectively and noninvasively stimulate individual
presynaptic terminals in rat brain slices. Unitary EPSPs failed to initiate a
spike in CA3 neurons under control conditions, but reliably discharged them after
induction of presynaptic short-term plasticity. Remarkably, PTP switched mossy
fiber synapses into full detonators for tens of seconds. Plasticity-dependent
detonation may be critical for efficient coding, storage, and recall of information
in the granule cell–CA3 cell network.
acknowledged_ssus:
- _id: M-Shop
- _id: PreCl
article_number: e17977
author:
- first_name: Nicholas
full_name: Vyleta, Nicholas
id: 36C4978E-F248-11E8-B48F-1D18A9856A87
last_name: Vyleta
- first_name: Carolina
full_name: Borges Merjane, Carolina
id: 4305C450-F248-11E8-B48F-1D18A9856A87
last_name: Borges Merjane
orcid: 0000-0003-0005-401X
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Vyleta N, Borges Merjane C, Jonas PM. Plasticity-dependent, full detonation
at hippocampal mossy fiber–CA3 pyramidal neuron synapses. eLife. 2016;5.
doi:10.7554/eLife.17977
apa: Vyleta, N., Borges Merjane, C., & Jonas, P. M. (2016). Plasticity-dependent,
full detonation at hippocampal mossy fiber–CA3 pyramidal neuron synapses. ELife.
eLife Sciences Publications. https://doi.org/10.7554/eLife.17977
chicago: Vyleta, Nicholas, Carolina Borges Merjane, and Peter M Jonas. “Plasticity-Dependent,
Full Detonation at Hippocampal Mossy Fiber–CA3 Pyramidal Neuron Synapses.” ELife.
eLife Sciences Publications, 2016. https://doi.org/10.7554/eLife.17977.
ieee: N. Vyleta, C. Borges Merjane, and P. M. Jonas, “Plasticity-dependent, full
detonation at hippocampal mossy fiber–CA3 pyramidal neuron synapses,” eLife,
vol. 5. eLife Sciences Publications, 2016.
ista: Vyleta N, Borges Merjane C, Jonas PM. 2016. Plasticity-dependent, full detonation
at hippocampal mossy fiber–CA3 pyramidal neuron synapses. eLife. 5, e17977.
mla: Vyleta, Nicholas, et al. “Plasticity-Dependent, Full Detonation at Hippocampal
Mossy Fiber–CA3 Pyramidal Neuron Synapses.” ELife, vol. 5, e17977, eLife
Sciences Publications, 2016, doi:10.7554/eLife.17977.
short: N. Vyleta, C. Borges Merjane, P.M. Jonas, ELife 5 (2016).
date_created: 2018-12-11T11:51:22Z
date_published: 2016-10-25T00:00:00Z
date_updated: 2023-02-21T10:34:24Z
day: '25'
ddc:
- '571'
- '572'
department:
- _id: PeJo
doi: 10.7554/eLife.17977
ec_funded: 1
file:
- access_level: open_access
checksum: a7201280c571bed88ebd459ce5ce6a47
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:17:05Z
date_updated: 2020-07-14T12:44:44Z
file_id: '5257'
file_name: IST-2016-715-v1+1_e17977-download.pdf
file_size: 1477891
relation: main_file
file_date_updated: 2020-07-14T12:44:44Z
has_accepted_license: '1'
intvolume: ' 5'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
project:
- _id: 25C0F108-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '268548'
name: Nanophysiology of fast-spiking, parvalbumin-expressing GABAergic interneurons
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glumatergic synapse
publication: eLife
publication_status: published
publisher: eLife Sciences Publications
publist_id: '5947'
pubrep_id: '715'
quality_controlled: '1'
scopus_import: 1
status: public
title: Plasticity-dependent, full detonation at hippocampal mossy fiber–CA3 pyramidal
neuron synapses
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 5
year: '2016'
...
---
_id: '1350'
abstract:
- lang: eng
text: "The hippocampal CA3 region plays a key role in learning and memory. Recurrent
CA3–CA3\r\nsynapses are thought to be the subcellular substrate of pattern completion.
However, the\r\nsynaptic mechanisms of this network computation remain enigmatic.
To investigate these mechanisms, we combined functional connectivity analysis
with network modeling.\r\nSimultaneous recording fromup to eight CA3 pyramidal
neurons revealed that connectivity was sparse, spatially uniform, and highly enriched
in disynaptic motifs (reciprocal, convergence,divergence, and chain motifs). Unitary
connections were composed of one or two synaptic contacts, suggesting efficient
use of postsynaptic space. Real-size modeling indicated that CA3 networks with
sparse connectivity, disynaptic motifs, and single-contact connections robustly
generated pattern completion.Thus, macro- and microconnectivity contribute to
efficient\r\nmemory storage and retrieval in hippocampal networks."
acknowledged_ssus:
- _id: ScienComp
author:
- first_name: José
full_name: Guzmán, José
id: 30CC5506-F248-11E8-B48F-1D18A9856A87
last_name: Guzmán
- first_name: Alois
full_name: Schlögl, Alois
id: 45BF87EE-F248-11E8-B48F-1D18A9856A87
last_name: Schlögl
orcid: 0000-0002-5621-8100
- first_name: Michael
full_name: Frotscher, Michael
last_name: Frotscher
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Guzmán J, Schlögl A, Frotscher M, Jonas PM. Synaptic mechanisms of pattern
completion in the hippocampal CA3 network. Science. 2016;353(6304):1117-1123.
doi:10.1126/science.aaf1836
apa: Guzmán, J., Schlögl, A., Frotscher, M., & Jonas, P. M. (2016). Synaptic
mechanisms of pattern completion in the hippocampal CA3 network. Science.
American Association for the Advancement of Science. https://doi.org/10.1126/science.aaf1836
chicago: Guzmán, José, Alois Schlögl, Michael Frotscher, and Peter M Jonas. “Synaptic
Mechanisms of Pattern Completion in the Hippocampal CA3 Network.” Science.
American Association for the Advancement of Science, 2016. https://doi.org/10.1126/science.aaf1836.
ieee: J. Guzmán, A. Schlögl, M. Frotscher, and P. M. Jonas, “Synaptic mechanisms
of pattern completion in the hippocampal CA3 network,” Science, vol. 353,
no. 6304. American Association for the Advancement of Science, pp. 1117–1123,
2016.
ista: Guzmán J, Schlögl A, Frotscher M, Jonas PM. 2016. Synaptic mechanisms of pattern
completion in the hippocampal CA3 network. Science. 353(6304), 1117–1123.
mla: Guzmán, José, et al. “Synaptic Mechanisms of Pattern Completion in the Hippocampal
CA3 Network.” Science, vol. 353, no. 6304, American Association for the
Advancement of Science, 2016, pp. 1117–23, doi:10.1126/science.aaf1836.
short: J. Guzmán, A. Schlögl, M. Frotscher, P.M. Jonas, Science 353 (2016) 1117–1123.
date_created: 2018-12-11T11:51:31Z
date_published: 2016-09-09T00:00:00Z
date_updated: 2021-01-12T06:50:04Z
day: '09'
ddc:
- '570'
department:
- _id: ScienComp
- _id: PeJo
doi: 10.1126/science.aaf1836
ec_funded: 1
file:
- access_level: open_access
checksum: 89caefa4e181424cbf0aecc835fcc5ec
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:12:27Z
date_updated: 2020-07-14T12:44:46Z
file_id: '4945'
file_name: IST-2017-823-v1+1_aaf1836_CombinedPDF_v2-1.pdf
file_size: 19408143
relation: main_file
file_date_updated: 2020-07-14T12:44:46Z
has_accepted_license: '1'
intvolume: ' 353'
issue: '6304'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Preprint
page: 1117 - 1123
project:
- _id: 25C0F108-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '268548'
name: Nanophysiology of fast-spiking, parvalbumin-expressing GABAergic interneurons
- _id: 25C26B1E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P24909-B24
name: Mechanisms of transmitter release at GABAergic synapses
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '5899'
pubrep_id: '823'
quality_controlled: '1'
scopus_import: 1
status: public
title: Synaptic mechanisms of pattern completion in the hippocampal CA3 network
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 353
year: '2016'
...
---
_id: '1435'
abstract:
- lang: eng
text: ATP released from neurons and astrocytes during neuronal activity or under
pathophysiological circumstances is able to influence information flow in neuronal
circuits by activation of ionotropic P2X and metabotropic P2Y receptors and subsequent
modulation of cellular excitability, synaptic strength, and plasticity. In the
present paper we review cellular and network effects of P2Y receptors in the brain.
We show that P2Y receptors inhibit the release of neurotransmitters, modulate
voltage- and ligand-gated ion channels, and differentially influence the induction
of synaptic plasticity in the prefrontal cortex, hippocampus, and cerebellum.
The findings discussed here may explain how P2Y1 receptor activation during brain
injury, hypoxia, inflammation, schizophrenia, or Alzheimer's disease leads to
an impairment of cognitive processes. Hence, it is suggested that the blockade
of P2Y1 receptors may have therapeutic potential against cognitive disturbances
in these states.
article_number: '1207393'
author:
- first_name: José
full_name: Guzmán, José
id: 30CC5506-F248-11E8-B48F-1D18A9856A87
last_name: Guzmán
- first_name: Zoltan
full_name: Gerevich, Zoltan
last_name: Gerevich
citation:
ama: 'Guzmán J, Gerevich Z. P2Y receptors in synaptic transmission and plasticity:
Therapeutic potential in cognitive dysfunction. Neural Plasticity. 2016;2016.
doi:10.1155/2016/1207393'
apa: 'Guzmán, J., & Gerevich, Z. (2016). P2Y receptors in synaptic transmission
and plasticity: Therapeutic potential in cognitive dysfunction. Neural Plasticity.
Hindawi Publishing Corporation. https://doi.org/10.1155/2016/1207393'
chicago: 'Guzmán, José, and Zoltan Gerevich. “P2Y Receptors in Synaptic Transmission
and Plasticity: Therapeutic Potential in Cognitive Dysfunction.” Neural Plasticity.
Hindawi Publishing Corporation, 2016. https://doi.org/10.1155/2016/1207393.'
ieee: 'J. Guzmán and Z. Gerevich, “P2Y receptors in synaptic transmission and plasticity:
Therapeutic potential in cognitive dysfunction,” Neural Plasticity, vol.
2016. Hindawi Publishing Corporation, 2016.'
ista: 'Guzmán J, Gerevich Z. 2016. P2Y receptors in synaptic transmission and plasticity:
Therapeutic potential in cognitive dysfunction. Neural Plasticity. 2016, 1207393.'
mla: 'Guzmán, José, and Zoltan Gerevich. “P2Y Receptors in Synaptic Transmission
and Plasticity: Therapeutic Potential in Cognitive Dysfunction.” Neural Plasticity,
vol. 2016, 1207393, Hindawi Publishing Corporation, 2016, doi:10.1155/2016/1207393.'
short: J. Guzmán, Z. Gerevich, Neural Plasticity 2016 (2016).
date_created: 2018-12-11T11:52:00Z
date_published: 2016-01-01T00:00:00Z
date_updated: 2021-01-12T06:50:43Z
day: '01'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1155/2016/1207393
file:
- access_level: open_access
checksum: 8dc5c2f3d44d4775a6e7e3edb0d7a0da
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:09:17Z
date_updated: 2020-07-14T12:44:54Z
file_id: '4740'
file_name: IST-2016-580-v1+1_1207393.pdf
file_size: 1395180
relation: main_file
file_date_updated: 2020-07-14T12:44:54Z
has_accepted_license: '1'
intvolume: ' 2016'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
publication: Neural Plasticity
publication_status: published
publisher: Hindawi Publishing Corporation
publist_id: '5762'
pubrep_id: '580'
quality_controlled: '1'
scopus_import: 1
status: public
title: 'P2Y receptors in synaptic transmission and plasticity: Therapeutic potential
in cognitive dysfunction'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 2016
year: '2016'
...
---
_id: '12903'
article_processing_charge: No
author:
- first_name: Alois
full_name: Schlögl, Alois
id: 45BF87EE-F248-11E8-B48F-1D18A9856A87
last_name: Schlögl
orcid: 0000-0002-5621-8100
- first_name: Stephan
full_name: Stadlbauer, Stephan
id: 4D0BC184-F248-11E8-B48F-1D18A9856A87
last_name: Stadlbauer
citation:
ama: 'Schlögl A, Stadlbauer S. High performance computing at IST Austria: Modelling
the human hippocampus. In: AHPC16 - Austrian HPC Meeting 2016. VSC - Vienna
Scientific Cluster; 2016:37.'
apa: 'Schlögl, A., & Stadlbauer, S. (2016). High performance computing at IST
Austria: Modelling the human hippocampus. In AHPC16 - Austrian HPC Meeting
2016 (p. 37). Grundlsee, Austria: VSC - Vienna Scientific Cluster.'
chicago: 'Schlögl, Alois, and Stephan Stadlbauer. “High Performance Computing at
IST Austria: Modelling the Human Hippocampus.” In AHPC16 - Austrian HPC Meeting
2016, 37. VSC - Vienna Scientific Cluster, 2016.'
ieee: 'A. Schlögl and S. Stadlbauer, “High performance computing at IST Austria:
Modelling the human hippocampus,” in AHPC16 - Austrian HPC Meeting 2016,
Grundlsee, Austria, 2016, p. 37.'
ista: 'Schlögl A, Stadlbauer S. 2016. High performance computing at IST Austria:
Modelling the human hippocampus. AHPC16 - Austrian HPC Meeting 2016. AHPC: Austrian
HPC Meeting, 37.'
mla: 'Schlögl, Alois, and Stephan Stadlbauer. “High Performance Computing at IST
Austria: Modelling the Human Hippocampus.” AHPC16 - Austrian HPC Meeting 2016,
VSC - Vienna Scientific Cluster, 2016, p. 37.'
short: A. Schlögl, S. Stadlbauer, in:, AHPC16 - Austrian HPC Meeting 2016, VSC -
Vienna Scientific Cluster, 2016, p. 37.
conference:
end_date: 2016-02-24
location: Grundlsee, Austria
name: 'AHPC: Austrian HPC Meeting'
start_date: 2016-02-22
date_created: 2023-05-05T12:54:47Z
date_published: 2016-02-24T00:00:00Z
date_updated: 2023-05-16T07:15:14Z
day: '24'
ddc:
- '000'
department:
- _id: ScienComp
- _id: PeJo
file:
- access_level: open_access
checksum: 4a7b00362e81358d568f5e216fa03c3e
content_type: application/pdf
creator: dernst
date_created: 2023-05-16T07:03:56Z
date_updated: 2023-05-16T07:03:56Z
file_id: '12968'
file_name: 2016_AHPC_Schloegl.pdf
file_size: 1073523
relation: main_file
success: 1
file_date_updated: 2023-05-16T07:03:56Z
has_accepted_license: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://vsc.ac.at/fileadmin/user_upload/vsc/conferences/ahpc16/BOOKLET_AHPC16.pdf
month: '02'
oa: 1
oa_version: Published Version
page: '37'
publication: AHPC16 - Austrian HPC Meeting 2016
publication_status: published
publisher: VSC - Vienna Scientific Cluster
quality_controlled: '1'
status: public
title: 'High performance computing at IST Austria: Modelling the human hippocampus'
type: conference_abstract
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2016'
...
---
_id: '1432'
abstract:
- lang: eng
text: CA3–CA3 recurrent excitatory synapses are thought to play a key role in memory
storage and pattern completion. Whether the plasticity properties of these synapses
are consistent with their proposed network functions remains unclear. Here, we
examine the properties of spike timing-dependent plasticity (STDP) at CA3–CA3
synapses. Low-frequency pairing of excitatory postsynaptic potentials (EPSPs)
and action potentials (APs) induces long-term potentiation (LTP), independent
of temporal order. The STDP curve is symmetric and broad (half-width ~150 ms).
Consistent with these STDP induction properties, AP–EPSP sequences lead to supralinear
summation of spine [Ca2+] transients. Furthermore, afterdepolarizations (ADPs)
following APs efficiently propagate into dendrites of CA3 pyramidal neurons, and
EPSPs summate with dendritic ADPs. In autoassociative network models, storage
and recall are more robust with symmetric than with asymmetric STDP rules. Thus,
a specialized STDP induction rule allows reliable storage and recall of information
in the hippocampal CA3 network.
acknowledgement: 'We thank Jozsef Csicsvari and Nelson Spruston for critically reading
the manuscript. We also thank A. Schlögl for programming, F. Marr for technical
assistance and E. Kramberger for manuscript editing. '
article_number: '11552'
author:
- first_name: Rajiv Kumar
full_name: Mishra, Rajiv Kumar
id: 46CB58F2-F248-11E8-B48F-1D18A9856A87
last_name: Mishra
- first_name: Sooyun
full_name: Kim, Sooyun
id: 394AB1C8-F248-11E8-B48F-1D18A9856A87
last_name: Kim
- first_name: José
full_name: Guzmán, José
id: 30CC5506-F248-11E8-B48F-1D18A9856A87
last_name: Guzmán
orcid: 0000-0003-2209-5242
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Mishra RK, Kim S, Guzmán J, Jonas PM. Symmetric spike timing-dependent plasticity
at CA3–CA3 synapses optimizes storage and recall in autoassociative networks.
Nature Communications. 2016;7. doi:10.1038/ncomms11552
apa: Mishra, R. K., Kim, S., Guzmán, J., & Jonas, P. M. (2016). Symmetric spike
timing-dependent plasticity at CA3–CA3 synapses optimizes storage and recall in
autoassociative networks. Nature Communications. Nature Publishing Group.
https://doi.org/10.1038/ncomms11552
chicago: Mishra, Rajiv Kumar, Sooyun Kim, José Guzmán, and Peter M Jonas. “Symmetric
Spike Timing-Dependent Plasticity at CA3–CA3 Synapses Optimizes Storage and Recall
in Autoassociative Networks.” Nature Communications. Nature Publishing
Group, 2016. https://doi.org/10.1038/ncomms11552.
ieee: R. K. Mishra, S. Kim, J. Guzmán, and P. M. Jonas, “Symmetric spike timing-dependent
plasticity at CA3–CA3 synapses optimizes storage and recall in autoassociative
networks,” Nature Communications, vol. 7. Nature Publishing Group, 2016.
ista: Mishra RK, Kim S, Guzmán J, Jonas PM. 2016. Symmetric spike timing-dependent
plasticity at CA3–CA3 synapses optimizes storage and recall in autoassociative
networks. Nature Communications. 7, 11552.
mla: Mishra, Rajiv Kumar, et al. “Symmetric Spike Timing-Dependent Plasticity at
CA3–CA3 Synapses Optimizes Storage and Recall in Autoassociative Networks.” Nature
Communications, vol. 7, 11552, Nature Publishing Group, 2016, doi:10.1038/ncomms11552.
short: R.K. Mishra, S. Kim, J. Guzmán, P.M. Jonas, Nature Communications 7 (2016).
date_created: 2018-12-11T11:51:59Z
date_published: 2016-05-13T00:00:00Z
date_updated: 2023-09-07T11:55:25Z
day: '13'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1038/ncomms11552
ec_funded: 1
file:
- access_level: open_access
checksum: 7e84d0392348c874d473b62f1042de22
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:18:33Z
date_updated: 2020-07-14T12:44:53Z
file_id: '5355'
file_name: IST-2016-582-v1+1_ncomms11552.pdf
file_size: 4510512
relation: main_file
file_date_updated: 2020-07-14T12:44:53Z
has_accepted_license: '1'
intvolume: ' 7'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
project:
- _id: 25C26B1E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P24909-B24
name: Mechanisms of transmitter release at GABAergic synapses
- _id: 25C0F108-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '268548'
name: Nanophysiology of fast-spiking, parvalbumin-expressing GABAergic interneurons
publication: Nature Communications
publication_status: published
publisher: Nature Publishing Group
publist_id: '5766'
pubrep_id: '582'
quality_controlled: '1'
related_material:
record:
- id: '1396'
relation: dissertation_contains
status: public
scopus_import: 1
status: public
title: Symmetric spike timing-dependent plasticity at CA3–CA3 synapses optimizes storage
and recall in autoassociative networks
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 7
year: '2016'
...
---
_id: '1396'
abstract:
- lang: eng
text: CA3 pyramidal neurons are thought to pay a key role in memory storage and
pattern completion by activity-dependent synaptic plasticity between CA3-CA3 recurrent
excitatory synapses. To examine the induction rules of synaptic plasticity at
CA3-CA3 synapses, we performed whole-cell patch-clamp recordings in acute hippocampal
slices from rats (postnatal 21-24 days) at room temperature. Compound excitatory
postsynaptic potentials (ESPSs) were recorded by tract stimulation in stratum
oriens in the presence of 10 µM gabazine. High-frequency stimulation (HFS) induced
N-methyl-D-aspartate (NMDA) receptor-dependent long-term potentiation (LTP). Although
LTP by HFS did not requier postsynaptic spikes, it was blocked by Na+-channel
blockers suggesting that local active processes (e.g.) dendritic spikes) may contribute
to LTP induction without requirement of a somatic action potential (AP). We next
examined the properties of spike timing-dependent plasticity (STDP) at CA3-CA3
synapses. Unexpectedly, low-frequency pairing of EPSPs and backpropagated action
potentialy (bAPs) induced LTP, independent of temporal order. The STDP curve was
symmetric and broad, with a half-width of ~150 ms. Consistent with these specific
STDP induction properties, post-presynaptic sequences led to a supralinear summation
of spine [Ca2+] transients. Furthermore, in autoassociative network models, storage
and recall was substantially more robust with symmetric than with asymmetric STDP
rules. In conclusion, we found associative forms of LTP at CA3-CA3 recurrent collateral
synapses with distinct induction rules. LTP induced by HFS may be associated with
dendritic spikes. In contrast, low frequency pairing of pre- and postsynaptic
activity induced LTP only if EPSP-AP were temporally very close. Together, these
induction mechanisms of synaptiic plasticity may contribute to memory storage
in the CA3-CA3 microcircuit at different ranges of activity.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Rajiv Kumar
full_name: Mishra, Rajiv Kumar
id: 46CB58F2-F248-11E8-B48F-1D18A9856A87
last_name: Mishra
citation:
ama: Mishra RK. Synaptic plasticity rules at CA3-CA3 recurrent synapses in hippocampus.
2016.
apa: Mishra, R. K. (2016). Synaptic plasticity rules at CA3-CA3 recurrent synapses
in hippocampus. Institute of Science and Technology Austria.
chicago: Mishra, Rajiv Kumar. “Synaptic Plasticity Rules at CA3-CA3 Recurrent Synapses
in Hippocampus.” Institute of Science and Technology Austria, 2016.
ieee: R. K. Mishra, “Synaptic plasticity rules at CA3-CA3 recurrent synapses in
hippocampus,” Institute of Science and Technology Austria, 2016.
ista: Mishra RK. 2016. Synaptic plasticity rules at CA3-CA3 recurrent synapses in
hippocampus. Institute of Science and Technology Austria.
mla: Mishra, Rajiv Kumar. Synaptic Plasticity Rules at CA3-CA3 Recurrent Synapses
in Hippocampus. Institute of Science and Technology Austria, 2016.
short: R.K. Mishra, Synaptic Plasticity Rules at CA3-CA3 Recurrent Synapses in Hippocampus,
Institute of Science and Technology Austria, 2016.
date_created: 2018-12-11T11:51:46Z
date_published: 2016-03-01T00:00:00Z
date_updated: 2023-09-07T11:55:26Z
day: '01'
ddc:
- '570'
degree_awarded: PhD
department:
- _id: PeJo
file:
- access_level: closed
checksum: 5a010a838faf040f7064f3cfb802f743
content_type: application/pdf
creator: dernst
date_created: 2019-08-09T12:14:46Z
date_updated: 2020-07-14T12:44:48Z
file_id: '6782'
file_name: Thesis_Mishra_Rajiv (Final).pdf
file_size: 2407572
relation: main_file
- access_level: open_access
checksum: 81b26d9ede92c99f1d8cc6fa1d04cbbb
content_type: application/pdf
creator: dernst
date_created: 2021-02-22T11:48:44Z
date_updated: 2021-02-22T11:48:44Z
file_id: '9183'
file_name: 2016_RajivMishra_Thesis.pdf
file_size: 2407572
relation: main_file
success: 1
file_date_updated: 2021-02-22T11:48:44Z
has_accepted_license: '1'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
page: '83'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '5811'
related_material:
record:
- id: '1432'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
title: Synaptic plasticity rules at CA3-CA3 recurrent synapses in hippocampus
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2016'
...
---
_id: '1616'
abstract:
- lang: eng
text: The hippocampus plays a key role in learning and memory. Previous studies
suggested that the main types of principal neurons, dentate gyrus granule cells
(GCs), CA3 pyramidal neurons, and CA1 pyramidal neurons, differ in their activity
pattern, with sparse firing in GCs and more frequent firing in CA3 and CA1 pyramidal
neurons. It has been assumed but never shown that such different activity may
be caused by differential synaptic excitation. To test this hypothesis, we performed
high-resolution whole-cell patch-clamp recordings in anesthetized rats in vivo.
In contrast to previous in vitro data, both CA3 and CA1 pyramidal neurons fired
action potentials spontaneously, with a frequency of ∼3–6 Hz, whereas GCs were
silent. Furthermore, both CA3 and CA1 cells primarily fired in bursts. To determine
the underlying mechanisms, we quantitatively assessed the frequency of spontaneous
excitatory synaptic input, the passive membrane properties, and the active membrane
characteristics. Surprisingly, GCs showed comparable synaptic excitation to CA3
and CA1 cells and the highest ratio of excitation versus hyperpolarizing inhibition.
Thus, differential synaptic excitation is not responsible for differences in firing.
Moreover, the three types of hippocampal neurons markedly differed in their passive
properties. While GCs showed the most negative membrane potential, CA3 pyramidal
neurons had the highest input resistance and the slowest membrane time constant.
The three types of neurons also differed in the active membrane characteristics.
GCs showed the highest action potential threshold, but displayed the largest gain
of the input-output curves. In conclusion, our results reveal that differential
firing of the three main types of hippocampal principal neurons in vivo is not
primarily caused by differences in the characteristics of the synaptic input,
but by the distinct properties of synaptic integration and input-output transformation.
acknowledgement: "The authors thank Jose Guzman for critically reading prior versions
of the manuscript. They also thank T. Asenov for\r\nengineering mechanical devices,
A. Schlögl for efficient pro-gramming, F. Marr for technical assistance, and E. Kramberger
for manuscript editing."
article_processing_charge: No
author:
- first_name: Janina
full_name: Kowalski, Janina
id: 3F3CA136-F248-11E8-B48F-1D18A9856A87
last_name: Kowalski
- first_name: Jian
full_name: Gan, Jian
id: 3614E438-F248-11E8-B48F-1D18A9856A87
last_name: Gan
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: Alejandro
full_name: Pernia-Andrade, Alejandro
id: 36963E98-F248-11E8-B48F-1D18A9856A87
last_name: Pernia-Andrade
citation:
ama: Kowalski J, Gan J, Jonas PM, Pernia-Andrade A. Intrinsic membrane properties
determine hippocampal differential firing pattern in vivo in anesthetized rats.
Hippocampus. 2016;26(5):668-682. doi:10.1002/hipo.22550
apa: Kowalski, J., Gan, J., Jonas, P. M., & Pernia-Andrade, A. (2016). Intrinsic
membrane properties determine hippocampal differential firing pattern in vivo
in anesthetized rats. Hippocampus. Wiley. https://doi.org/10.1002/hipo.22550
chicago: Kowalski, Janina, Jian Gan, Peter M Jonas, and Alejandro Pernia-Andrade.
“Intrinsic Membrane Properties Determine Hippocampal Differential Firing Pattern
in Vivo in Anesthetized Rats.” Hippocampus. Wiley, 2016. https://doi.org/10.1002/hipo.22550.
ieee: J. Kowalski, J. Gan, P. M. Jonas, and A. Pernia-Andrade, “Intrinsic membrane
properties determine hippocampal differential firing pattern in vivo in anesthetized
rats,” Hippocampus, vol. 26, no. 5. Wiley, pp. 668–682, 2016.
ista: Kowalski J, Gan J, Jonas PM, Pernia-Andrade A. 2016. Intrinsic membrane properties
determine hippocampal differential firing pattern in vivo in anesthetized rats.
Hippocampus. 26(5), 668–682.
mla: Kowalski, Janina, et al. “Intrinsic Membrane Properties Determine Hippocampal
Differential Firing Pattern in Vivo in Anesthetized Rats.” Hippocampus,
vol. 26, no. 5, Wiley, 2016, pp. 668–82, doi:10.1002/hipo.22550.
short: J. Kowalski, J. Gan, P.M. Jonas, A. Pernia-Andrade, Hippocampus 26 (2016)
668–682.
date_created: 2018-12-11T11:53:03Z
date_published: 2016-05-01T00:00:00Z
date_updated: 2023-10-17T10:02:02Z
day: '01'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1002/hipo.22550
file:
- access_level: open_access
checksum: 284b72b12fbe15474833ed3d4549f86b
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:13:47Z
date_updated: 2020-07-14T12:45:07Z
file_id: '5033'
file_name: IST-2016-469-v1+1_Kowalski_et_al-Hippocampus.pdf
file_size: 905348
relation: main_file
file_date_updated: 2020-07-14T12:45:07Z
has_accepted_license: '1'
intvolume: ' 26'
issue: '5'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
page: 668 - 682
publication: Hippocampus
publication_identifier:
eissn:
- 1098-1063
issn:
- 1050-9631
publication_status: published
publisher: Wiley
publist_id: '5550'
pubrep_id: '469'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Intrinsic membrane properties determine hippocampal differential firing pattern
in vivo in anesthetized rats
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 26
year: '2016'
...
---
_id: '1535'
abstract:
- lang: eng
text: Neuronal and neuroendocrine L-type calcium channels (Cav1.2, Cav1.3) open
readily at relatively low membrane potentials and allow Ca2+ to enter the cells
near resting potentials. In this way, Cav1.2 and Cav1.3 shape the action potential
waveform, contribute to gene expression, synaptic plasticity, neuronal differentiation,
hormone secretion and pacemaker activity. In the chromaffin cells (CCs) of the
adrenal medulla, Cav1.3 is highly expressed and is shown to support most of the
pacemaking current that sustains action potential (AP) firings and part of the
catecholamine secretion. Cav1.3 forms Ca2+-nanodomains with the fast inactivating
BK channels and drives the resting SK currents. These latter set the inter-spike
interval duration between consecutive spikes during spontaneous firing and the
rate of spike adaptation during sustained depolarizations. Cav1.3 plays also a
primary role in the switch from “tonic” to “burst” firing that occurs in mouse
CCs when either the availability of voltage-gated Na channels (Nav) is reduced
or the β2 subunit featuring the fast inactivating BK channels is deleted. Here,
we discuss the functional role of these “neuronlike” firing modes in CCs and how
Cav1.3 contributes to them. The open issue is to understand how these novel firing
patterns are adapted to regulate the quantity of circulating catecholamines during
resting condition or in response to acute and chronic stress.
acknowledgement: This work was supported by the Italian MIUR (PRIN 2010/2011 project
2010JFYFY2) and the University of Torino.
article_processing_charge: No
article_type: original
author:
- first_name: David H
full_name: Vandael, David H
id: 3AE48E0A-F248-11E8-B48F-1D18A9856A87
last_name: Vandael
orcid: 0000-0001-7577-1676
- first_name: Andrea
full_name: Marcantoni, Andrea
last_name: Marcantoni
- first_name: Emilio
full_name: Carbone, Emilio
last_name: Carbone
citation:
ama: Vandael DH, Marcantoni A, Carbone E. Cav1.3 channels as key regulators of neuron-like
firings and catecholamine release in chromaffin cells. Current Molecular Pharmacology.
2015;8(2):149-161. doi:10.2174/1874467208666150507105443
apa: Vandael, D. H., Marcantoni, A., & Carbone, E. (2015). Cav1.3 channels as
key regulators of neuron-like firings and catecholamine release in chromaffin
cells. Current Molecular Pharmacology. Bentham Science Publishers. https://doi.org/10.2174/1874467208666150507105443
chicago: Vandael, David H, Andrea Marcantoni, and Emilio Carbone. “Cav1.3 Channels
as Key Regulators of Neuron-like Firings and Catecholamine Release in Chromaffin
Cells.” Current Molecular Pharmacology. Bentham Science Publishers, 2015.
https://doi.org/10.2174/1874467208666150507105443.
ieee: D. H. Vandael, A. Marcantoni, and E. Carbone, “Cav1.3 channels as key regulators
of neuron-like firings and catecholamine release in chromaffin cells,” Current
Molecular Pharmacology, vol. 8, no. 2. Bentham Science Publishers, pp. 149–161,
2015.
ista: Vandael DH, Marcantoni A, Carbone E. 2015. Cav1.3 channels as key regulators
of neuron-like firings and catecholamine release in chromaffin cells. Current
Molecular Pharmacology. 8(2), 149–161.
mla: Vandael, David H., et al. “Cav1.3 Channels as Key Regulators of Neuron-like
Firings and Catecholamine Release in Chromaffin Cells.” Current Molecular Pharmacology,
vol. 8, no. 2, Bentham Science Publishers, 2015, pp. 149–61, doi:10.2174/1874467208666150507105443.
short: D.H. Vandael, A. Marcantoni, E. Carbone, Current Molecular Pharmacology 8
(2015) 149–161.
date_created: 2018-12-11T11:52:35Z
date_published: 2015-10-01T00:00:00Z
date_updated: 2021-01-12T06:51:26Z
day: '01'
department:
- _id: PeJo
doi: 10.2174/1874467208666150507105443
external_id:
pmid:
- '25966692'
intvolume: ' 8'
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5384372/
month: '10'
oa: 1
oa_version: Submitted Version
page: 149 - 161
pmid: 1
publication: Current Molecular Pharmacology
publication_status: published
publisher: Bentham Science Publishers
publist_id: '5636'
quality_controlled: '1'
scopus_import: 1
status: public
title: Cav1.3 channels as key regulators of neuron-like firings and catecholamine
release in chromaffin cells
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 8
year: '2015'
...
---
_id: '1565'
abstract:
- lang: eng
text: Leptin is an adipokine produced by the adipose tissue regulating body weight
through its appetite-suppressing effect. Besides being expressed in the hypothalamus
and hippocampus, leptin receptors (ObRs) are also present in chromaffin cells
of the adrenal medulla. In the present study, we report the effect of leptin on
mouse chromaffin cell (MCC) functionality, focusing on cell excitability and catecholamine
secretion. Acute application of leptin (1 nm) on spontaneously firing MCCs caused
a slowly developing membrane hyperpolarization followed by complete blockade of
action potential (AP) firing. This inhibitory effect at rest was abolished by
the BK channel blocker paxilline (1 μm), suggesting the involvement of BK potassium
channels. Single-channel recordings in 'perforated microvesicles' confirmed that
leptin increased BK channel open probability without altering its unitary conductance.
BK channel up-regulation was associated with the phosphoinositide 3-kinase (PI3K)
signalling cascade because the PI3K specific inhibitor wortmannin (100 nm) fully
prevented BK current increase. We also tested the effect of leptin on evoked AP
firing and Ca2+-driven exocytosis. Although leptin preserves well-adapted AP trains
of lower frequency, APs are broader and depolarization-evoked exocytosis is increased
as a result of the larger size of the ready-releasable pool and higher frequency
of vesicle release. The kinetics and quantal size of single secretory events remained
unaltered. Leptin had no effect on firing and secretion in db-/db- mice lacking
the ObR gene, confirming its specificity. In conclusion, leptin exhibits a dual
action on MCC activity. It dampens AP firing at rest but preserves AP firing and
increases catecholamine secretion during sustained stimulation, highlighting the
importance of the adipo-adrenal axis in the leptin-mediated increase of sympathetic
tone and catecholamine release.
acknowledgement: "This work was supported by the Compagnia di San Paolo Foundation
‘Neuroscience Program’ to VC and ‘Progetto di Ateneo 2011-13’ to EC.\r\nWe thank
Dr Claudio Franchino for cell preparation and for providing excellent technical
support."
author:
- first_name: Daniela
full_name: Gavello, Daniela
last_name: Gavello
- first_name: David H
full_name: Vandael, David H
id: 3AE48E0A-F248-11E8-B48F-1D18A9856A87
last_name: Vandael
orcid: 0000-0001-7577-1676
- first_name: Sara
full_name: Gosso, Sara
last_name: Gosso
- first_name: Emilio
full_name: Carbone, Emilio
last_name: Carbone
- first_name: Valentina
full_name: Carabelli, Valentina
last_name: Carabelli
citation:
ama: Gavello D, Vandael DH, Gosso S, Carbone E, Carabelli V. Dual action of leptin
on rest-firing and stimulated catecholamine release via phosphoinositide 3-kinase-riven
BK channel up-regulation in mouse chromaffin cells. Journal of Physiology.
2015;593(22):4835-4853. doi:10.1113/JP271078
apa: Gavello, D., Vandael, D. H., Gosso, S., Carbone, E., & Carabelli, V. (2015).
Dual action of leptin on rest-firing and stimulated catecholamine release via
phosphoinositide 3-kinase-riven BK channel up-regulation in mouse chromaffin cells.
Journal of Physiology. Wiley-Blackwell. https://doi.org/10.1113/JP271078
chicago: Gavello, Daniela, David H Vandael, Sara Gosso, Emilio Carbone, and Valentina
Carabelli. “Dual Action of Leptin on Rest-Firing and Stimulated Catecholamine
Release via Phosphoinositide 3-Kinase-Riven BK Channel up-Regulation in Mouse
Chromaffin Cells.” Journal of Physiology. Wiley-Blackwell, 2015. https://doi.org/10.1113/JP271078.
ieee: D. Gavello, D. H. Vandael, S. Gosso, E. Carbone, and V. Carabelli, “Dual action
of leptin on rest-firing and stimulated catecholamine release via phosphoinositide
3-kinase-riven BK channel up-regulation in mouse chromaffin cells,” Journal
of Physiology, vol. 593, no. 22. Wiley-Blackwell, pp. 4835–4853, 2015.
ista: Gavello D, Vandael DH, Gosso S, Carbone E, Carabelli V. 2015. Dual action
of leptin on rest-firing and stimulated catecholamine release via phosphoinositide
3-kinase-riven BK channel up-regulation in mouse chromaffin cells. Journal of
Physiology. 593(22), 4835–4853.
mla: Gavello, Daniela, et al. “Dual Action of Leptin on Rest-Firing and Stimulated
Catecholamine Release via Phosphoinositide 3-Kinase-Riven BK Channel up-Regulation
in Mouse Chromaffin Cells.” Journal of Physiology, vol. 593, no. 22, Wiley-Blackwell,
2015, pp. 4835–53, doi:10.1113/JP271078.
short: D. Gavello, D.H. Vandael, S. Gosso, E. Carbone, V. Carabelli, Journal of
Physiology 593 (2015) 4835–4853.
date_created: 2018-12-11T11:52:45Z
date_published: 2015-11-15T00:00:00Z
date_updated: 2021-01-12T06:51:38Z
day: '15'
department:
- _id: PeJo
doi: 10.1113/JP271078
external_id:
pmid:
- '26282459'
intvolume: ' 593'
issue: '22'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4650409/
month: '11'
oa: 1
oa_version: Submitted Version
page: 4835 - 4853
pmid: 1
publication: Journal of Physiology
publication_status: published
publisher: Wiley-Blackwell
publist_id: '5606'
quality_controlled: '1'
scopus_import: 1
status: public
title: Dual action of leptin on rest-firing and stimulated catecholamine release via
phosphoinositide 3-kinase-riven BK channel up-regulation in mouse chromaffin cells
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 593
year: '2015'
...
---
_id: '1580'
abstract:
- lang: eng
text: Synapsins (Syns) are an evolutionarily conserved family of presynaptic proteins
crucial for the fine-tuning of synaptic function. A large amount of experimental
evidences has shown that Syns are involved in the development of epileptic phenotypes
and several mutations in Syn genes have been associated with epilepsy in humans
and animal models. Syn mutations induce alterations in circuitry and neurotransmitter
release, differentially affecting excitatory and inhibitory synapses, thus causing
an excitation/inhibition imbalance in network excitability toward hyperexcitability
that may be a determinant with regard to the development of epilepsy. Another
approach to investigate epileptogenic mechanisms is to understand how silencing
Syn affects the cellular behavior of single neurons and is associated with the
hyperexcitable phenotypes observed in epilepsy. Here, we examined the functional
effects of antisense-RNA inhibition of Syn expression on individually identified
and isolated serotonergic cells of the Helix land snail. We found that Helix synapsin
silencing increases cell excitability characterized by a slightly depolarized
resting membrane potential, decreases the rheobase, reduces the threshold for
action potential (AP) firing and increases the mean and instantaneous firing rates,
with respect to control cells. The observed increase of Ca2+ and BK currents in
Syn-silenced cells seems to be related to changes in the shape of the AP waveform.
These currents sustain the faster spiking in Syn-deficient cells by increasing
the after hyperpolarization and limiting the Na+ and Ca2+ channel inactivation
during repetitive firing. This in turn speeds up the depolarization phase by reaching
the AP threshold faster. Our results provide evidence that Syn silencing increases
intrinsic cell excitability associated with increased Ca2+ and Ca2+-dependent
BK currents in the absence of excitatory or inhibitory inputs.
article_processing_charge: No
article_type: original
author:
- first_name: Oscar
full_name: Brenes, Oscar
last_name: Brenes
- first_name: David H
full_name: Vandael, David H
id: 3AE48E0A-F248-11E8-B48F-1D18A9856A87
last_name: Vandael
orcid: 0000-0001-7577-1676
- first_name: Emilio
full_name: Carbone, Emilio
last_name: Carbone
- first_name: Pier
full_name: Montarolo, Pier
last_name: Montarolo
- first_name: Mirella
full_name: Ghirardi, Mirella
last_name: Ghirardi
citation:
ama: Brenes O, Vandael DH, Carbone E, Montarolo P, Ghirardi M. Knock-down of synapsin
alters cell excitability and action potential waveform by potentiating BK and
voltage gated Ca2 currents in Helix serotonergic neurons. Neuroscience.
2015;311:430-443. doi:10.1016/j.neuroscience.2015.10.046
apa: Brenes, O., Vandael, D. H., Carbone, E., Montarolo, P., & Ghirardi, M.
(2015). Knock-down of synapsin alters cell excitability and action potential waveform
by potentiating BK and voltage gated Ca2 currents in Helix serotonergic neurons.
Neuroscience. Elsevier. https://doi.org/10.1016/j.neuroscience.2015.10.046
chicago: Brenes, Oscar, David H Vandael, Emilio Carbone, Pier Montarolo, and Mirella
Ghirardi. “Knock-down of Synapsin Alters Cell Excitability and Action Potential
Waveform by Potentiating BK and Voltage Gated Ca2 Currents in Helix Serotonergic
Neurons.” Neuroscience. Elsevier, 2015. https://doi.org/10.1016/j.neuroscience.2015.10.046.
ieee: O. Brenes, D. H. Vandael, E. Carbone, P. Montarolo, and M. Ghirardi, “Knock-down
of synapsin alters cell excitability and action potential waveform by potentiating
BK and voltage gated Ca2 currents in Helix serotonergic neurons,” Neuroscience,
vol. 311. Elsevier, pp. 430–443, 2015.
ista: Brenes O, Vandael DH, Carbone E, Montarolo P, Ghirardi M. 2015. Knock-down
of synapsin alters cell excitability and action potential waveform by potentiating
BK and voltage gated Ca2 currents in Helix serotonergic neurons. Neuroscience.
311, 430–443.
mla: Brenes, Oscar, et al. “Knock-down of Synapsin Alters Cell Excitability and
Action Potential Waveform by Potentiating BK and Voltage Gated Ca2 Currents in
Helix Serotonergic Neurons.” Neuroscience, vol. 311, Elsevier, 2015, pp.
430–43, doi:10.1016/j.neuroscience.2015.10.046.
short: O. Brenes, D.H. Vandael, E. Carbone, P. Montarolo, M. Ghirardi, Neuroscience
311 (2015) 430–443.
date_created: 2018-12-11T11:52:50Z
date_published: 2015-12-17T00:00:00Z
date_updated: 2021-01-12T06:51:44Z
day: '17'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1016/j.neuroscience.2015.10.046
file:
- access_level: open_access
checksum: af2c4c994718c7be417eba0dc746aac9
content_type: application/pdf
creator: dernst
date_created: 2020-05-15T06:50:20Z
date_updated: 2020-07-14T12:45:02Z
file_id: '7849'
file_name: 2015_Neuroscience_Brenes.pdf
file_size: 5563015
relation: main_file
file_date_updated: 2020-07-14T12:45:02Z
has_accepted_license: '1'
intvolume: ' 311'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Submitted Version
page: 430 - 443
publication: Neuroscience
publication_status: published
publisher: Elsevier
publist_id: '5591'
quality_controlled: '1'
scopus_import: 1
status: public
title: Knock-down of synapsin alters cell excitability and action potential waveform
by potentiating BK and voltage gated Ca2 currents in Helix serotonergic neurons
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 311
year: '2015'
...
---
_id: '1615'
abstract:
- lang: eng
text: Loss-of-function mutations in the synaptic adhesion protein Neuroligin-4 are
among the most common genetic abnormalities associated with autism spectrum disorders,
but little is known about the function of Neuroligin-4 and the consequences of
its loss. We assessed synaptic and network characteristics in Neuroligin-4 knockout
mice, focusing on the hippocampus as a model brain region with a critical role
in cognition and memory, and found that Neuroligin-4 deletion causes subtle defects
of the protein composition and function of GABAergic synapses in the hippocampal
CA3 region. Interestingly, these subtle synaptic changes are accompanied by pronounced
perturbations of γ-oscillatory network activity, which has been implicated in
cognitive function and is altered in multiple psychiatric and neurodevelopmental
disorders. Our data provide important insights into the mechanisms by which Neuroligin-4-dependent
GABAergic synapses may contribute to autism phenotypes and indicate new strategies
for therapeutic approaches.
acknowledgement: This work was supported by the Max Planck Society (N.B. and H.E.),
the European Commission (EU-AIMS FP7-115300, N.B. and H.E.; Marie Curie IRG, D.K.-B.),
the German Research Foundation (CNMPB, N.B., H.E., and F.V.), the Alexander von
Humboldt-Foundation (D.K.-B.), and the Austrian Fond zur Förderung der Wissenschaftlichen
Forschung (P 24909-B24, P.J.). M.H. was a student of the doctoral program Molecular
Physiology of the Brain. Dr. J.-M. Fritschy generously provided the GABAARγ2 antibody.
We thank F. Benseler, I. Thanhäuser, D. Schwerdtfeger, A. Ronnenberg, and D. Winkler
for valuable advice and excellent technical support. We are grateful to the staff
at the animal facility of the Max Planck Institute of Experimental Medicine for
mouse husbandry.
author:
- first_name: Matthieu
full_name: Hammer, Matthieu
last_name: Hammer
- first_name: Dilja
full_name: Krueger Burg, Dilja
last_name: Krueger Burg
- first_name: Liam
full_name: Tuffy, Liam
last_name: Tuffy
- first_name: Benjamin
full_name: Cooper, Benjamin
last_name: Cooper
- first_name: Holger
full_name: Taschenberger, Holger
last_name: Taschenberger
- first_name: Sarit
full_name: Goswami, Sarit
id: 3A578F32-F248-11E8-B48F-1D18A9856A87
last_name: Goswami
- first_name: Hannelore
full_name: Ehrenreich, Hannelore
last_name: Ehrenreich
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: Frederique
full_name: Varoqueaux, Frederique
last_name: Varoqueaux
- first_name: Jeong
full_name: Rhee, Jeong
last_name: Rhee
- first_name: Nils
full_name: Brose, Nils
last_name: Brose
citation:
ama: Hammer M, Krueger Burg D, Tuffy L, et al. Perturbed hippocampal synaptic inhibition
and γ-oscillations in a neuroligin-4 knockout mouse model of autism. Cell Reports.
2015;13(3):516-523. doi:10.1016/j.celrep.2015.09.011
apa: Hammer, M., Krueger Burg, D., Tuffy, L., Cooper, B., Taschenberger, H., Goswami,
S., … Brose, N. (2015). Perturbed hippocampal synaptic inhibition and γ-oscillations
in a neuroligin-4 knockout mouse model of autism. Cell Reports. Cell Press.
https://doi.org/10.1016/j.celrep.2015.09.011
chicago: Hammer, Matthieu, Dilja Krueger Burg, Liam Tuffy, Benjamin Cooper, Holger
Taschenberger, Sarit Goswami, Hannelore Ehrenreich, et al. “Perturbed Hippocampal
Synaptic Inhibition and γ-Oscillations in a Neuroligin-4 Knockout Mouse Model
of Autism.” Cell Reports. Cell Press, 2015. https://doi.org/10.1016/j.celrep.2015.09.011.
ieee: M. Hammer et al., “Perturbed hippocampal synaptic inhibition and γ-oscillations
in a neuroligin-4 knockout mouse model of autism,” Cell Reports, vol. 13,
no. 3. Cell Press, pp. 516–523, 2015.
ista: Hammer M, Krueger Burg D, Tuffy L, Cooper B, Taschenberger H, Goswami S, Ehrenreich
H, Jonas PM, Varoqueaux F, Rhee J, Brose N. 2015. Perturbed hippocampal synaptic
inhibition and γ-oscillations in a neuroligin-4 knockout mouse model of autism.
Cell Reports. 13(3), 516–523.
mla: Hammer, Matthieu, et al. “Perturbed Hippocampal Synaptic Inhibition and γ-Oscillations
in a Neuroligin-4 Knockout Mouse Model of Autism.” Cell Reports, vol. 13,
no. 3, Cell Press, 2015, pp. 516–23, doi:10.1016/j.celrep.2015.09.011.
short: M. Hammer, D. Krueger Burg, L. Tuffy, B. Cooper, H. Taschenberger, S. Goswami,
H. Ehrenreich, P.M. Jonas, F. Varoqueaux, J. Rhee, N. Brose, Cell Reports 13 (2015)
516–523.
date_created: 2018-12-11T11:53:02Z
date_published: 2015-10-20T00:00:00Z
date_updated: 2021-01-12T06:52:01Z
day: '20'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1016/j.celrep.2015.09.011
file:
- access_level: open_access
checksum: 44d30fbb543774b076b4938bd36af9d7
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:13:23Z
date_updated: 2020-07-14T12:45:07Z
file_id: '5005'
file_name: IST-2016-470-v1+1_1-s2.0-S2211124715010220-main.pdf
file_size: 2314406
relation: main_file
file_date_updated: 2020-07-14T12:45:07Z
has_accepted_license: '1'
intvolume: ' 13'
issue: '3'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
page: 516 - 523
publication: Cell Reports
publication_status: published
publisher: Cell Press
publist_id: '5551'
pubrep_id: '470'
quality_controlled: '1'
scopus_import: 1
status: public
title: Perturbed hippocampal synaptic inhibition and γ-oscillations in a neuroligin-4
knockout mouse model of autism
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 13
year: '2015'
...
---
_id: '1614'
abstract:
- lang: eng
text: 'GABAergic perisoma-inhibiting fast-spiking interneurons (PIIs) effectively
control the activity of large neuron populations by their wide axonal arborizations.
It is generally assumed that the output of one PII to its target cells is strong
and rapid. Here, we show that, unexpectedly, both strength and time course of
PII-mediated perisomatic inhibition change with distance between synaptically
connected partners in the rodent hippocampus. Synaptic signals become weaker due
to lower contact numbers and decay more slowly with distance, very likely resulting
from changes in GABAA receptor subunit composition. When distance-dependent synaptic
inhibition is introduced to a rhythmically active neuronal network model, randomly
driven principal cell assemblies are strongly synchronized by the PIIs, leading
to higher precision in principal cell spike times than in a network with uniform
synaptic inhibition. '
author:
- first_name: Michael
full_name: Strüber, Michael
last_name: Strüber
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: Marlene
full_name: Bartos, Marlene
last_name: Bartos
citation:
ama: Strüber M, Jonas PM, Bartos M. Strength and duration of perisomatic GABAergic
inhibition depend on distance between synaptically connected cells. PNAS.
2015;112(4):1220-1225. doi:10.1073/pnas.1412996112
apa: Strüber, M., Jonas, P. M., & Bartos, M. (2015). Strength and duration of
perisomatic GABAergic inhibition depend on distance between synaptically connected
cells. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1412996112
chicago: Strüber, Michael, Peter M Jonas, and Marlene Bartos. “Strength and Duration
of Perisomatic GABAergic Inhibition Depend on Distance between Synaptically Connected
Cells.” PNAS. National Academy of Sciences, 2015. https://doi.org/10.1073/pnas.1412996112.
ieee: M. Strüber, P. M. Jonas, and M. Bartos, “Strength and duration of perisomatic
GABAergic inhibition depend on distance between synaptically connected cells,”
PNAS, vol. 112, no. 4. National Academy of Sciences, pp. 1220–1225, 2015.
ista: Strüber M, Jonas PM, Bartos M. 2015. Strength and duration of perisomatic
GABAergic inhibition depend on distance between synaptically connected cells.
PNAS. 112(4), 1220–1225.
mla: Strüber, Michael, et al. “Strength and Duration of Perisomatic GABAergic Inhibition
Depend on Distance between Synaptically Connected Cells.” PNAS, vol. 112,
no. 4, National Academy of Sciences, 2015, pp. 1220–25, doi:10.1073/pnas.1412996112.
short: M. Strüber, P.M. Jonas, M. Bartos, PNAS 112 (2015) 1220–1225.
date_created: 2018-12-11T11:53:02Z
date_published: 2015-01-27T00:00:00Z
date_updated: 2021-01-12T06:52:01Z
day: '27'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1073/pnas.1412996112
ec_funded: 1
external_id:
pmid:
- '25583495'
file:
- access_level: open_access
checksum: 6703309a1f58493cf5a704211fb6ebed
content_type: application/pdf
creator: dernst
date_created: 2019-01-17T07:52:40Z
date_updated: 2020-07-14T12:45:07Z
file_id: '5838'
file_name: 2015_PNAS_Strueber.pdf
file_size: 1280860
relation: main_file
file_date_updated: 2020-07-14T12:45:07Z
has_accepted_license: '1'
intvolume: ' 112'
issue: '4'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: 1220 - 1225
pmid: 1
project:
- _id: 25C26B1E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P24909-B24
name: Mechanisms of transmitter release at GABAergic synapses
- _id: 25C0F108-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '268548'
name: Nanophysiology of fast-spiking, parvalbumin-expressing GABAergic interneurons
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '5552'
quality_controlled: '1'
scopus_import: 1
status: public
title: Strength and duration of perisomatic GABAergic inhibition depend on distance
between synaptically connected cells
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 112
year: '2015'
...
---
_id: '1845'
abstract:
- lang: eng
text: Based on extrapolation from excitatory synapses, it is often assumed that
depletion of the releasable pool of synaptic vesicles is the main factor underlying
depression at inhibitory synapses. In this issue of Neuron, using subcellular
patch-clamp recording from inhibitory presynaptic terminals, Kawaguchi and Sakaba
(2015) show that at Purkinje cell-deep cerebellar nuclei neuron synapses, changes
in presynaptic action potential waveform substantially contribute to synaptic
depression. Based on extrapolation from excitatory synapses, it is often assumed
that depletion of the releasable pool of synaptic vesicles is the main factor
underlying depression at inhibitory synapses. In this issue of Neuron, using subcellular
patch-clamp recording from inhibitory presynaptic terminals, Kawaguchi and Sakaba
(2015) show that at Purkinje cell-deep cerebellar nuclei neuron synapses, changes
in presynaptic action potential waveform substantially contribute to synaptic
depression.
article_processing_charge: No
author:
- first_name: David H
full_name: Vandael, David H
id: 3AE48E0A-F248-11E8-B48F-1D18A9856A87
last_name: Vandael
orcid: 0000-0001-7577-1676
- first_name: 'Claudia '
full_name: 'Espinoza Martinez, Claudia '
id: 31FFEE2E-F248-11E8-B48F-1D18A9856A87
last_name: Espinoza Martinez
orcid: 0000-0003-4710-2082
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Vandael DH, Espinoza Martinez C, Jonas PM. Excitement about inhibitory presynaptic
terminals. Neuron. 2015;85(6):1149-1151. doi:10.1016/j.neuron.2015.03.006
apa: Vandael, D. H., Espinoza Martinez, C., & Jonas, P. M. (2015). Excitement
about inhibitory presynaptic terminals. Neuron. Elsevier. https://doi.org/10.1016/j.neuron.2015.03.006
chicago: Vandael, David H, Claudia Espinoza Martinez, and Peter M Jonas. “Excitement
about Inhibitory Presynaptic Terminals.” Neuron. Elsevier, 2015. https://doi.org/10.1016/j.neuron.2015.03.006.
ieee: D. H. Vandael, C. Espinoza Martinez, and P. M. Jonas, “Excitement about inhibitory
presynaptic terminals,” Neuron, vol. 85, no. 6. Elsevier, pp. 1149–1151,
2015.
ista: Vandael DH, Espinoza Martinez C, Jonas PM. 2015. Excitement about inhibitory
presynaptic terminals. Neuron. 85(6), 1149–1151.
mla: Vandael, David H., et al. “Excitement about Inhibitory Presynaptic Terminals.”
Neuron, vol. 85, no. 6, Elsevier, 2015, pp. 1149–51, doi:10.1016/j.neuron.2015.03.006.
short: D.H. Vandael, C. Espinoza Martinez, P.M. Jonas, Neuron 85 (2015) 1149–1151.
date_created: 2018-12-11T11:54:19Z
date_published: 2015-03-18T00:00:00Z
date_updated: 2021-10-08T09:07:34Z
day: '18'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1016/j.neuron.2015.03.006
file:
- access_level: open_access
checksum: d1808550e376a0eca2a950fda017cfa6
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:16:07Z
date_updated: 2020-07-14T12:45:19Z
file_id: '5192'
file_name: IST-2017-822-v1+1_Perspective_Fig__Final.pdf
file_size: 411832
relation: main_file
- access_level: open_access
checksum: a279f4ae61e6c8f33d68f69a0d02097d
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:16:07Z
date_updated: 2020-07-14T12:45:19Z
file_id: '5193'
file_name: IST-2017-822-v1+2_Perspective_Final2.pdf
file_size: 100769
relation: main_file
file_date_updated: 2020-07-14T12:45:19Z
has_accepted_license: '1'
intvolume: ' 85'
issue: '6'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
page: 1149 - 1151
publication: Neuron
publication_status: published
publisher: Elsevier
publist_id: '5256'
pubrep_id: '822'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Excitement about inhibitory presynaptic terminals
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: journal_article
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
volume: 85
year: '2015'
...
---
_id: '1834'
abstract:
- lang: eng
text: Huge body of evidences demonstrated that volatile anesthetics affect the hippocampal
neurogenesis and neurocognitive functions, and most of them showed impairment
at anesthetic dose. Here, we investigated the effect of low dose (1.8%) sevoflurane
on hippocampal neurogenesis and dentate gyrus-dependent learning. Neonatal rats
at postnatal day 4 to 6 (P4-6) were treated with 1.8% sevoflurane for 6 hours.
Neurogenesis was quantified by bromodeoxyuridine labeling and electrophysiology
recording. Four and seven weeks after treatment, the Morris water maze and contextual-fear
discrimination learning tests were performed to determine the influence on spatial
learning and pattern separation. A 6-hour treatment with 1.8% sevoflurane promoted
hippocampal neurogenesis and increased the survival of newborn cells and the proportion
of immature granular cells in the dentate gyrus of neonatal rats. Sevoflurane-treated
rats performed better during the training days of the Morris water maze test and
in contextual-fear discrimination learning test. These results suggest that a
subanesthetic dose of sevoflurane promotes hippocampal neurogenesis in neonatal
rats and facilitates their performance in dentate gyrus-dependent learning tasks.
article_processing_charge: No
article_type: original
author:
- first_name: Chong
full_name: Chen, Chong
id: 3DFD581A-F248-11E8-B48F-1D18A9856A87
last_name: Chen
- first_name: Chao
full_name: Wang, Chao
last_name: Wang
- first_name: Xuan
full_name: Zhao, Xuan
last_name: Zhao
- first_name: Tao
full_name: Zhou, Tao
last_name: Zhou
- first_name: Dao
full_name: Xu, Dao
last_name: Xu
- first_name: Zhi
full_name: Wang, Zhi
last_name: Wang
- first_name: Ying
full_name: Wang, Ying
last_name: Wang
citation:
ama: Chen C, Wang C, Zhao X, et al. Low-dose sevoflurane promoteshippocampal neurogenesis
and facilitates the development of dentate gyrus-dependent learning in neonatal
rats. ASN Neuro. 2015;7(2). doi:10.1177/1759091415575845
apa: Chen, C., Wang, C., Zhao, X., Zhou, T., Xu, D., Wang, Z., & Wang, Y. (2015).
Low-dose sevoflurane promoteshippocampal neurogenesis and facilitates the development
of dentate gyrus-dependent learning in neonatal rats. ASN Neuro. SAGE Publications.
https://doi.org/10.1177/1759091415575845
chicago: Chen, Chong, Chao Wang, Xuan Zhao, Tao Zhou, Dao Xu, Zhi Wang, and Ying
Wang. “Low-Dose Sevoflurane Promoteshippocampal Neurogenesis and Facilitates the
Development of Dentate Gyrus-Dependent Learning in Neonatal Rats.” ASN Neuro.
SAGE Publications, 2015. https://doi.org/10.1177/1759091415575845.
ieee: C. Chen et al., “Low-dose sevoflurane promoteshippocampal neurogenesis
and facilitates the development of dentate gyrus-dependent learning in neonatal
rats,” ASN Neuro, vol. 7, no. 2. SAGE Publications, 2015.
ista: Chen C, Wang C, Zhao X, Zhou T, Xu D, Wang Z, Wang Y. 2015. Low-dose sevoflurane
promoteshippocampal neurogenesis and facilitates the development of dentate gyrus-dependent
learning in neonatal rats. ASN Neuro. 7(2).
mla: Chen, Chong, et al. “Low-Dose Sevoflurane Promoteshippocampal Neurogenesis
and Facilitates the Development of Dentate Gyrus-Dependent Learning in Neonatal
Rats.” ASN Neuro, vol. 7, no. 2, SAGE Publications, 2015, doi:10.1177/1759091415575845.
short: C. Chen, C. Wang, X. Zhao, T. Zhou, D. Xu, Z. Wang, Y. Wang, ASN Neuro 7
(2015).
date_created: 2018-12-11T11:54:16Z
date_published: 2015-04-13T00:00:00Z
date_updated: 2023-10-18T06:47:30Z
day: '13'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1177/1759091415575845
file:
- access_level: open_access
checksum: 53e16bd3fc2ae2c0d7de9164626c37aa
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:14:08Z
date_updated: 2020-07-14T12:45:18Z
file_id: '5057'
file_name: IST-2016-456-v1+1_ASN_Neuro-2015-Chen-.pdf
file_size: 1146814
relation: main_file
file_date_updated: 2020-07-14T12:45:18Z
has_accepted_license: '1'
intvolume: ' 7'
issue: '2'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/3.0/
month: '04'
oa: 1
oa_version: Published Version
publication: ASN Neuro
publication_status: published
publisher: SAGE Publications
publist_id: '5269'
pubrep_id: '456'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Low-dose sevoflurane promoteshippocampal neurogenesis and facilitates the development
of dentate gyrus-dependent learning in neonatal rats
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/3.0/legalcode
name: Creative Commons Attribution 3.0 Unported (CC BY 3.0)
short: CC BY (3.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 7
year: '2015'
...
---
_id: '1890'
abstract:
- lang: eng
text: To search for a target in a complex environment is an everyday behavior that
ends with finding the target. When we search for two identical targets, however,
we must continue the search after finding the first target and memorize its location.
We used fixation-related potentials to investigate the neural correlates of different
stages of the search, that is, before and after finding the first target. Having
found the first target influenced subsequent distractor processing. Compared to
distractor fixations before the first target fixation, a negative shift was observed
for three subsequent distractor fixations. These results suggest that processing
a target in continued search modulates the brain's response, either transiently
by reflecting temporary working memory processes or permanently by reflecting
working memory retention.
acknowledgement: 'Funded by Austrian Science Fund (FWF) Grant Number: P 22189-B18;
European Union within the 6th Framework Programme Grant Number: 517590; State government
of Styria Grant Number: PN 4055'
author:
- first_name: Christof
full_name: Körner, Christof
last_name: Körner
- first_name: Verena
full_name: Braunstein, Verena
last_name: Braunstein
- first_name: Matthias
full_name: Stangl, Matthias
last_name: Stangl
- first_name: Alois
full_name: Schlögl, Alois
id: 45BF87EE-F248-11E8-B48F-1D18A9856A87
last_name: Schlögl
orcid: 0000-0002-5621-8100
- first_name: Christa
full_name: Neuper, Christa
last_name: Neuper
- first_name: Anja
full_name: Ischebeck, Anja
last_name: Ischebeck
citation:
ama: 'Körner C, Braunstein V, Stangl M, Schlögl A, Neuper C, Ischebeck A. Sequential
effects in continued visual search: Using fixation-related potentials to compare
distractor processing before and after target detection. Psychophysiology.
2014;51(4):385-395. doi:10.1111/psyp.12062'
apa: 'Körner, C., Braunstein, V., Stangl, M., Schlögl, A., Neuper, C., & Ischebeck,
A. (2014). Sequential effects in continued visual search: Using fixation-related
potentials to compare distractor processing before and after target detection.
Psychophysiology. Wiley-Blackwell. https://doi.org/10.1111/psyp.12062'
chicago: 'Körner, Christof, Verena Braunstein, Matthias Stangl, Alois Schlögl, Christa
Neuper, and Anja Ischebeck. “Sequential Effects in Continued Visual Search: Using
Fixation-Related Potentials to Compare Distractor Processing before and after
Target Detection.” Psychophysiology. Wiley-Blackwell, 2014. https://doi.org/10.1111/psyp.12062.'
ieee: 'C. Körner, V. Braunstein, M. Stangl, A. Schlögl, C. Neuper, and A. Ischebeck,
“Sequential effects in continued visual search: Using fixation-related potentials
to compare distractor processing before and after target detection,” Psychophysiology,
vol. 51, no. 4. Wiley-Blackwell, pp. 385–395, 2014.'
ista: 'Körner C, Braunstein V, Stangl M, Schlögl A, Neuper C, Ischebeck A. 2014.
Sequential effects in continued visual search: Using fixation-related potentials
to compare distractor processing before and after target detection. Psychophysiology.
51(4), 385–395.'
mla: 'Körner, Christof, et al. “Sequential Effects in Continued Visual Search: Using
Fixation-Related Potentials to Compare Distractor Processing before and after
Target Detection.” Psychophysiology, vol. 51, no. 4, Wiley-Blackwell, 2014,
pp. 385–95, doi:10.1111/psyp.12062.'
short: C. Körner, V. Braunstein, M. Stangl, A. Schlögl, C. Neuper, A. Ischebeck,
Psychophysiology 51 (2014) 385–395.
date_created: 2018-12-11T11:54:34Z
date_published: 2014-02-11T00:00:00Z
date_updated: 2021-01-12T06:53:52Z
day: '11'
ddc:
- '000'
department:
- _id: ScienComp
- _id: PeJo
doi: 10.1111/psyp.12062
file:
- access_level: open_access
checksum: 4255b6185e774acce1d99f8e195c564d
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:16:44Z
date_updated: 2020-07-14T12:45:20Z
file_id: '5233'
file_name: IST-2016-442-v1+1_K-rner_et_al-2014-Psychophysiology.pdf
file_size: 543243
relation: main_file
file_date_updated: 2020-07-14T12:45:20Z
has_accepted_license: '1'
intvolume: ' 51'
issue: '4'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: 385 - 395
publication: Psychophysiology
publication_status: published
publisher: Wiley-Blackwell
publist_id: '5205'
pubrep_id: '442'
scopus_import: 1
status: public
title: 'Sequential effects in continued visual search: Using fixation-related potentials
to compare distractor processing before and after target detection'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 51
year: '2014'
...
---
_id: '2002'
abstract:
- lang: eng
text: Oriens-lacunosum moleculare (O-LM) interneurons in the CA1 region of the hippocampus
play a key role in feedback inhibition and in the control of network activity.
However, how these cells are efficiently activated in the network remains unclear.
To address this question, I performed recordings from CA1 pyramidal neuron axons,
the presynaptic fibers that provide feedback innervation of these interneurons.
Two forms of axonal action potential (AP) modulation were identified. First, repetitive
stimulation resulted in activity-dependent AP broadening. Broadening showed fast
onset, with marked changes in AP shape following a single AP. Second, tonic depolarization
in CA1 pyramidal neuron somata induced AP broadening in the axon, and depolarization-induced
broadening summated with activity-dependent broadening. Outsideout patch recordings
from CA1 pyramidal neuron axons revealed a high density of a-dendrotoxin (α-DTX)-sensitive,
inactivating K+ channels, suggesting that K+ channel inactivation mechanistically
contributes to AP broadening. To examine the functional consequences of axonal
AP modulation for synaptic transmission, I performed paired recordings between
synaptically connected CA1 pyramidal neurons and O-LM interneurons. CA1 pyramidal
neuron-O-LM interneuron excitatory postsynaptic currents (EPSCs) showed facilitation
during both repetitive stimulation and tonic depolarization of the presynaptic
neuron. Both effects were mimicked and occluded by α-DTX, suggesting that they
were mediated by K+ channel inactivation. Therefore, axonal AP modulation can
greatly facilitate the activation of O-LM interneurons. In conclusion, modulation
of AP shape in CA1 pyramidal neuron axons substantially enhances the efficacy
of principal neuron-interneuron synapses, promoting the activation of O-LM interneurons
in recurrent inhibitory microcircuits.
article_number: '0113124'
author:
- first_name: Sooyun
full_name: Kim, Sooyun
id: 394AB1C8-F248-11E8-B48F-1D18A9856A87
last_name: Kim
citation:
ama: Kim S. Action potential modulation in CA1 pyramidal neuron axons facilitates
OLM interneuron activation in recurrent inhibitory microcircuits of rat hippocampus.
PLoS One. 2014;9(11). doi:10.1371/journal.pone.0113124
apa: Kim, S. (2014). Action potential modulation in CA1 pyramidal neuron axons facilitates
OLM interneuron activation in recurrent inhibitory microcircuits of rat hippocampus.
PLoS One. Public Library of Science. https://doi.org/10.1371/journal.pone.0113124
chicago: Kim, Sooyun. “Action Potential Modulation in CA1 Pyramidal Neuron Axons
Facilitates OLM Interneuron Activation in Recurrent Inhibitory Microcircuits of
Rat Hippocampus.” PLoS One. Public Library of Science, 2014. https://doi.org/10.1371/journal.pone.0113124.
ieee: S. Kim, “Action potential modulation in CA1 pyramidal neuron axons facilitates
OLM interneuron activation in recurrent inhibitory microcircuits of rat hippocampus,”
PLoS One, vol. 9, no. 11. Public Library of Science, 2014.
ista: Kim S. 2014. Action potential modulation in CA1 pyramidal neuron axons facilitates
OLM interneuron activation in recurrent inhibitory microcircuits of rat hippocampus.
PLoS One. 9(11), 0113124.
mla: Kim, Sooyun. “Action Potential Modulation in CA1 Pyramidal Neuron Axons Facilitates
OLM Interneuron Activation in Recurrent Inhibitory Microcircuits of Rat Hippocampus.”
PLoS One, vol. 9, no. 11, 0113124, Public Library of Science, 2014, doi:10.1371/journal.pone.0113124.
short: S. Kim, PLoS One 9 (2014).
date_created: 2018-12-11T11:55:09Z
date_published: 2014-11-19T00:00:00Z
date_updated: 2021-01-12T06:54:39Z
day: '19'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1371/journal.pone.0113124
ec_funded: 1
file:
- access_level: open_access
checksum: 85e4f4ea144f827272aaf376b2830564
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:14:52Z
date_updated: 2020-07-14T12:45:24Z
file_id: '5107'
file_name: IST-2016-434-v1+1_journal.pone.0113124.pdf
file_size: 5179993
relation: main_file
file_date_updated: 2020-07-14T12:45:24Z
has_accepted_license: '1'
intvolume: ' 9'
issue: '11'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-sa/4.0/
month: '11'
oa: 1
oa_version: Published Version
project:
- _id: 25C0F108-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '268548'
name: Nanophysiology of fast-spiking, parvalbumin-expressing GABAergic interneurons
publication: PLoS One
publication_status: published
publisher: Public Library of Science
publist_id: '5074'
pubrep_id: '434'
quality_controlled: '1'
scopus_import: 1
status: public
title: Action potential modulation in CA1 pyramidal neuron axons facilitates OLM interneuron
activation in recurrent inhibitory microcircuits of rat hippocampus
tmp:
image: /images/cc_by_sa.png
legal_code_url: https://creativecommons.org/licenses/by-sa/4.0/legalcode
name: Creative Commons Attribution-ShareAlike 4.0 International Public License (CC
BY-SA 4.0)
short: CC BY-SA (4.0)
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 9
year: '2014'
...
---
_id: '2031'
abstract:
- lang: eng
text: A puzzling property of synaptic transmission, originally established at the
neuromuscular junction, is that the time course of transmitter release is independent
of the extracellular Ca2+ concentration ([Ca2+]o), whereas the rate of release
is highly [Ca2+]o-dependent. Here, we examine the time course of release at inhibitory
basket cell-Purkinje cell synapses and show that it is independent of [Ca2+]o.
Modeling of Ca2+-dependent transmitter release suggests that the invariant time
course of release critically depends on tight coupling between Ca2+ channels and
release sensors. Experiments with exogenous Ca2+ chelators reveal that channel-sensor
coupling at basket cell-Purkinje cell synapses is very tight, with a mean distance
of 10–20 nm. Thus, tight channel-sensor coupling provides a mechanistic explanation
for the apparent [Ca2+]o independence of the time course of release.
author:
- first_name: Itaru
full_name: Arai, Itaru
id: 32A73F6C-F248-11E8-B48F-1D18A9856A87
last_name: Arai
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Arai itaru, Jonas PM. Nanodomain coupling explains Ca^2+ independence of transmitter
release time course at a fast central synapse. eLife. 2014;3. doi:10.7554/eLife.04057
apa: Arai, itaru, & Jonas, P. M. (2014). Nanodomain coupling explains Ca^2+
independence of transmitter release time course at a fast central synapse. ELife.
eLife Sciences Publications. https://doi.org/10.7554/eLife.04057
chicago: Arai, itaru, and Peter M Jonas. “Nanodomain Coupling Explains Ca^2+ Independence
of Transmitter Release Time Course at a Fast Central Synapse.” ELife. eLife
Sciences Publications, 2014. https://doi.org/10.7554/eLife.04057.
ieee: itaru Arai and P. M. Jonas, “Nanodomain coupling explains Ca^2+ independence
of transmitter release time course at a fast central synapse,” eLife, vol.
3. eLife Sciences Publications, 2014.
ista: Arai itaru, Jonas PM. 2014. Nanodomain coupling explains Ca^2+ independence
of transmitter release time course at a fast central synapse. eLife. 3.
mla: Arai, itaru, and Peter M. Jonas. “Nanodomain Coupling Explains Ca^2+ Independence
of Transmitter Release Time Course at a Fast Central Synapse.” ELife, vol.
3, eLife Sciences Publications, 2014, doi:10.7554/eLife.04057.
short: itaru Arai, P.M. Jonas, ELife 3 (2014).
date_created: 2018-12-11T11:55:19Z
date_published: 2014-12-09T00:00:00Z
date_updated: 2021-01-12T06:54:51Z
day: '09'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.7554/eLife.04057
ec_funded: 1
file:
- access_level: open_access
checksum: c240f915450d4ebe8f95043a2a8c7b1a
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:14:41Z
date_updated: 2020-07-14T12:45:26Z
file_id: '5094'
file_name: IST-2016-421-v1+1_e04057.full.pdf
file_size: 2239563
relation: main_file
file_date_updated: 2020-07-14T12:45:26Z
has_accepted_license: '1'
intvolume: ' 3'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Submitted Version
project:
- _id: 25C26B1E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P24909-B24
name: Mechanisms of transmitter release at GABAergic synapses
- _id: 25C0F108-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '268548'
name: Nanophysiology of fast-spiking, parvalbumin-expressing GABAergic interneurons
publication: eLife
publication_status: published
publisher: eLife Sciences Publications
publist_id: '5041'
pubrep_id: '421'
quality_controlled: '1'
scopus_import: 1
status: public
title: Nanodomain coupling explains Ca^2+ independence of transmitter release time
course at a fast central synapse
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 3
year: '2014'
...
---
_id: '2041'
abstract:
- lang: eng
text: The hippocampus mediates several higher brain functions, such as learning,
memory, and spatial coding. The input region of the hippocampus, the dentate gyrus,
plays a critical role in these processes. Several lines of evidence suggest that
the dentate gyrus acts as a preprocessor of incoming information, preparing it
for subsequent processing in CA3. For example, the dentate gyrus converts input
from the entorhinal cortex, where cells have multiple spatial fields, into the
spatially more specific place cell activity characteristic of the CA3 region.
Furthermore, the dentate gyrus is involved in pattern separation, transforming
relatively similar input patterns into substantially different output patterns.
Finally, the dentate gyrus produces a very sparse coding scheme in which only
a very small fraction of neurons are active at any one time.
article_number: 2p
author:
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: John
full_name: Lisman, John
last_name: Lisman
citation:
ama: Jonas PM, Lisman J. Structure, function and plasticity of hippocampal dentate
gyrus microcircuits. Frontiers in Neural Circuits. 2014;8. doi:10.3389/fncir.2014.00107
apa: Jonas, P. M., & Lisman, J. (2014). Structure, function and plasticity of
hippocampal dentate gyrus microcircuits. Frontiers in Neural Circuits.
Frontiers Research Foundation. https://doi.org/10.3389/fncir.2014.00107
chicago: Jonas, Peter M, and John Lisman. “Structure, Function and Plasticity of
Hippocampal Dentate Gyrus Microcircuits.” Frontiers in Neural Circuits.
Frontiers Research Foundation, 2014. https://doi.org/10.3389/fncir.2014.00107.
ieee: P. M. Jonas and J. Lisman, “Structure, function and plasticity of hippocampal
dentate gyrus microcircuits,” Frontiers in Neural Circuits, vol. 8. Frontiers
Research Foundation, 2014.
ista: Jonas PM, Lisman J. 2014. Structure, function and plasticity of hippocampal
dentate gyrus microcircuits. Frontiers in Neural Circuits. 8, 2p.
mla: Jonas, Peter M., and John Lisman. “Structure, Function and Plasticity of Hippocampal
Dentate Gyrus Microcircuits.” Frontiers in Neural Circuits, vol. 8, 2p,
Frontiers Research Foundation, 2014, doi:10.3389/fncir.2014.00107.
short: P.M. Jonas, J. Lisman, Frontiers in Neural Circuits 8 (2014).
date_created: 2018-12-11T11:55:22Z
date_published: 2014-09-10T00:00:00Z
date_updated: 2021-01-12T06:54:55Z
day: '10'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.3389/fncir.2014.00107
file:
- access_level: open_access
checksum: 3ca57b164045523f876407e9f13a9fb8
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:17:38Z
date_updated: 2020-07-14T12:45:26Z
file_id: '5294'
file_name: IST-2016-424-v1+1_fncir-08-00107.pdf
file_size: 201110
relation: main_file
file_date_updated: 2020-07-14T12:45:26Z
has_accepted_license: '1'
intvolume: ' 8'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
publication: Frontiers in Neural Circuits
publication_status: published
publisher: Frontiers Research Foundation
publist_id: '5010'
pubrep_id: '424'
quality_controlled: '1'
scopus_import: 1
status: public
title: Structure, function and plasticity of hippocampal dentate gyrus microcircuits
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 8
year: '2014'
...
---
_id: '2062'
abstract:
- lang: eng
text: The success story of fast-spiking, parvalbumin-positive (PV+) GABAergic interneurons
(GABA, γ-aminobutyric acid) in the mammalian central nervous system is noteworthy.
In 1995, the properties of these interneurons were completely unknown. Twenty
years later, thanks to the massive use of subcellular patch-clamp techniques,
simultaneous multiple-cell recording, optogenetics, in vivo measurements, and
computational approaches, our knowledge about PV+ interneurons became more extensive
than for several types of pyramidal neurons. These findings have implications
beyond the “small world” of basic research on GABAergic cells. For example, the
results provide a first proof of principle that neuroscientists might be able
to close the gaps between the molecular, cellular, network, and behavioral levels,
representing one of the main challenges at the present time. Furthermore, the
results may form the basis for PV+ interneurons as therapeutic targets for brain
disease in the future. However, much needs to be learned about the basic function
of these interneurons before clinical neuroscientists will be able to use PV+
interneurons for therapeutic purposes.
article_number: '1255263'
author:
- first_name: Hua
full_name: Hu, Hua
id: 4AC0145C-F248-11E8-B48F-1D18A9856A87
last_name: Hu
- first_name: Jian
full_name: Gan, Jian
id: 3614E438-F248-11E8-B48F-1D18A9856A87
last_name: Gan
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: 'Hu H, Gan J, Jonas PM. Fast-spiking parvalbumin^+ GABAergic interneurons:
From cellular design to microcircuit function. Science. 2014;345(6196).
doi:10.1126/science.1255263'
apa: 'Hu, H., Gan, J., & Jonas, P. M. (2014). Fast-spiking parvalbumin^+ GABAergic
interneurons: From cellular design to microcircuit function. Science. American
Association for the Advancement of Science. https://doi.org/10.1126/science.1255263'
chicago: 'Hu, Hua, Jian Gan, and Peter M Jonas. “Fast-Spiking Parvalbumin^+ GABAergic
Interneurons: From Cellular Design to Microcircuit Function.” Science.
American Association for the Advancement of Science, 2014. https://doi.org/10.1126/science.1255263.'
ieee: 'H. Hu, J. Gan, and P. M. Jonas, “Fast-spiking parvalbumin^+ GABAergic interneurons:
From cellular design to microcircuit function,” Science, vol. 345, no.
6196. American Association for the Advancement of Science, 2014.'
ista: 'Hu H, Gan J, Jonas PM. 2014. Fast-spiking parvalbumin^+ GABAergic interneurons:
From cellular design to microcircuit function. Science. 345(6196), 1255263.'
mla: 'Hu, Hua, et al. “Fast-Spiking Parvalbumin^+ GABAergic Interneurons: From Cellular
Design to Microcircuit Function.” Science, vol. 345, no. 6196, 1255263,
American Association for the Advancement of Science, 2014, doi:10.1126/science.1255263.'
short: H. Hu, J. Gan, P.M. Jonas, Science 345 (2014).
date_created: 2018-12-11T11:55:29Z
date_published: 2014-08-01T00:00:00Z
date_updated: 2021-01-12T06:55:03Z
day: '01'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1126/science.1255263
ec_funded: 1
file:
- access_level: open_access
checksum: a0036a589037d37e86364fa25cc0a82f
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:16:00Z
date_updated: 2020-07-14T12:45:27Z
file_id: '5185'
file_name: IST-2017-821-v1+1_1255263JonasPVReviewTextR_Final.pdf
file_size: 215514
relation: main_file
- access_level: open_access
checksum: e1f57d2713725449cb898fdcb8ef47b8
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:16:01Z
date_updated: 2020-07-14T12:45:27Z
file_id: '5186'
file_name: IST-2017-821-v1+2_1255263JonasPVReviewFigures_Final.pdf
file_size: 1732723
relation: main_file
file_date_updated: 2020-07-14T12:45:27Z
has_accepted_license: '1'
intvolume: ' 345'
issue: '6196'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Submitted Version
project:
- _id: 25C26B1E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P24909-B24
name: Mechanisms of transmitter release at GABAergic synapses
- _id: 25C0F108-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '268548'
name: Nanophysiology of fast-spiking, parvalbumin-expressing GABAergic interneurons
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '4984'
pubrep_id: '821'
quality_controlled: '1'
scopus_import: 1
status: public
title: 'Fast-spiking parvalbumin^+ GABAergic interneurons: From cellular design to
microcircuit function'
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 345
year: '2014'
...
---
_id: '2164'
abstract:
- lang: eng
text: 'Neuronal ectopia, such as granule cell dispersion (GCD) in temporal lobe
epilepsy (TLE), has been assumed to result from a migration defect during development.
Indeed, recent studies reported that aberrant migration of neonatal-generated
dentate granule cells (GCs) increased the risk to develop epilepsy later in life.
On the contrary, in the present study, we show that fully differentiated GCs become
motile following the induction of epileptiform activity, resulting in GCD. Hippocampal
slice cultures from transgenic mice expressing green fluorescent protein in differentiated,
but not in newly generated GCs, were incubated with the glutamate receptor agonist
kainate (KA), which induced GC burst activity and GCD. Using real-time microscopy,
we observed that KA-exposed, differentiated GCs translocated their cell bodies
and changed their dendritic organization. As found in human TLE, KA application
was associated with decreased expression of the extracellular matrix protein Reelin,
particularly in hilar interneurons. Together these findings suggest that KA-induced
motility of differentiated GCs contributes to the development of GCD and establish
slice cultures as a model to study neuronal changes induced by epileptiform activity. '
author:
- first_name: Xuejun
full_name: Chai, Xuejun
last_name: Chai
- first_name: Gert
full_name: Münzner, Gert
last_name: Münzner
- first_name: Shanting
full_name: Zhao, Shanting
last_name: Zhao
- first_name: Stefanie
full_name: Tinnes, Stefanie
last_name: Tinnes
- first_name: Janina
full_name: Kowalski, Janina
id: 3F3CA136-F248-11E8-B48F-1D18A9856A87
last_name: Kowalski
- first_name: Ute
full_name: Häussler, Ute
last_name: Häussler
- first_name: Christina
full_name: Young, Christina
last_name: Young
- first_name: Carola
full_name: Haas, Carola
last_name: Haas
- first_name: Michael
full_name: Frotscher, Michael
last_name: Frotscher
citation:
ama: Chai X, Münzner G, Zhao S, et al. Epilepsy-induced motility of differentiated
neurons. Cerebral Cortex. 2014;24(8):2130-2140. doi:10.1093/cercor/bht067
apa: Chai, X., Münzner, G., Zhao, S., Tinnes, S., Kowalski, J., Häussler, U., …
Frotscher, M. (2014). Epilepsy-induced motility of differentiated neurons. Cerebral
Cortex. Oxford University Press. https://doi.org/10.1093/cercor/bht067
chicago: Chai, Xuejun, Gert Münzner, Shanting Zhao, Stefanie Tinnes, Janina Kowalski,
Ute Häussler, Christina Young, Carola Haas, and Michael Frotscher. “Epilepsy-Induced
Motility of Differentiated Neurons.” Cerebral Cortex. Oxford University
Press, 2014. https://doi.org/10.1093/cercor/bht067.
ieee: X. Chai et al., “Epilepsy-induced motility of differentiated neurons,”
Cerebral Cortex, vol. 24, no. 8. Oxford University Press, pp. 2130–2140,
2014.
ista: Chai X, Münzner G, Zhao S, Tinnes S, Kowalski J, Häussler U, Young C, Haas
C, Frotscher M. 2014. Epilepsy-induced motility of differentiated neurons. Cerebral
Cortex. 24(8), 2130–2140.
mla: Chai, Xuejun, et al. “Epilepsy-Induced Motility of Differentiated Neurons.”
Cerebral Cortex, vol. 24, no. 8, Oxford University Press, 2014, pp. 2130–40,
doi:10.1093/cercor/bht067.
short: X. Chai, G. Münzner, S. Zhao, S. Tinnes, J. Kowalski, U. Häussler, C. Young,
C. Haas, M. Frotscher, Cerebral Cortex 24 (2014) 2130–2140.
date_created: 2018-12-11T11:56:04Z
date_published: 2014-08-01T00:00:00Z
date_updated: 2021-01-12T06:55:43Z
day: '01'
department:
- _id: PeJo
doi: 10.1093/cercor/bht067
intvolume: ' 24'
issue: '8'
language:
- iso: eng
month: '08'
oa_version: None
page: 2130 - 2140
publication: Cerebral Cortex
publication_status: published
publisher: Oxford University Press
publist_id: '4820'
quality_controlled: '1'
scopus_import: 1
status: public
title: Epilepsy-induced motility of differentiated neurons
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 24
year: '2014'
...
---
_id: '2176'
abstract:
- lang: eng
text: Electron microscopy (EM) allows for the simultaneous visualization of all
tissue components at high resolution. However, the extent to which conventional
aldehyde fixation and ethanol dehydration of the tissue alter the fine structure
of cells and organelles, thereby preventing detection of subtle structural changes
induced by an experiment, has remained an issue. Attempts have been made to rapidly
freeze tissue to preserve native ultrastructure. Shock-freezing of living tissue
under high pressure (high-pressure freezing, HPF) followed by cryosubstitution
of the tissue water avoids aldehyde fixation and dehydration in ethanol; the tissue
water is immobilized in â ̂1/450 ms, and a close-to-native fine structure of cells,
organelles and molecules is preserved. Here we describe a protocol for HPF that
is useful to monitor ultrastructural changes associated with functional changes
at synapses in the brain but can be applied to many other tissues as well. The
procedure requires a high-pressure freezer and takes a minimum of 7 d but can
be paused at several points.
author:
- first_name: Daniel
full_name: Studer, Daniel
last_name: Studer
- first_name: Shanting
full_name: Zhao, Shanting
last_name: Zhao
- first_name: Xuejun
full_name: Chai, Xuejun
last_name: Chai
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: Werner
full_name: Graber, Werner
last_name: Graber
- first_name: Sigrun
full_name: Nestel, Sigrun
last_name: Nestel
- first_name: Michael
full_name: Frotscher, Michael
last_name: Frotscher
citation:
ama: Studer D, Zhao S, Chai X, et al. Capture of activity-induced ultrastructural
changes at synapses by high-pressure freezing of brain tissue. Nature Protocols.
2014;9(6):1480-1495. doi:10.1038/nprot.2014.099
apa: Studer, D., Zhao, S., Chai, X., Jonas, P. M., Graber, W., Nestel, S., &
Frotscher, M. (2014). Capture of activity-induced ultrastructural changes at synapses
by high-pressure freezing of brain tissue. Nature Protocols. Nature Publishing
Group. https://doi.org/10.1038/nprot.2014.099
chicago: Studer, Daniel, Shanting Zhao, Xuejun Chai, Peter M Jonas, Werner Graber,
Sigrun Nestel, and Michael Frotscher. “Capture of Activity-Induced Ultrastructural
Changes at Synapses by High-Pressure Freezing of Brain Tissue.” Nature Protocols.
Nature Publishing Group, 2014. https://doi.org/10.1038/nprot.2014.099.
ieee: D. Studer et al., “Capture of activity-induced ultrastructural changes
at synapses by high-pressure freezing of brain tissue,” Nature Protocols,
vol. 9, no. 6. Nature Publishing Group, pp. 1480–1495, 2014.
ista: Studer D, Zhao S, Chai X, Jonas PM, Graber W, Nestel S, Frotscher M. 2014.
Capture of activity-induced ultrastructural changes at synapses by high-pressure
freezing of brain tissue. Nature Protocols. 9(6), 1480–1495.
mla: Studer, Daniel, et al. “Capture of Activity-Induced Ultrastructural Changes
at Synapses by High-Pressure Freezing of Brain Tissue.” Nature Protocols,
vol. 9, no. 6, Nature Publishing Group, 2014, pp. 1480–95, doi:10.1038/nprot.2014.099.
short: D. Studer, S. Zhao, X. Chai, P.M. Jonas, W. Graber, S. Nestel, M. Frotscher,
Nature Protocols 9 (2014) 1480–1495.
date_created: 2018-12-11T11:56:09Z
date_published: 2014-05-29T00:00:00Z
date_updated: 2021-01-12T06:55:47Z
day: '29'
department:
- _id: PeJo
doi: 10.1038/nprot.2014.099
intvolume: ' 9'
issue: '6'
language:
- iso: eng
month: '05'
oa_version: None
page: 1480 - 1495
project:
- _id: 25BDE9A4-B435-11E9-9278-68D0E5697425
grant_number: SFB-TR3-TP10B
name: Glutamaterge synaptische Übertragung und Plastizität in hippocampalen Mikroschaltkreisen
publication: Nature Protocols
publication_status: published
publisher: Nature Publishing Group
publist_id: '4807'
quality_controlled: '1'
scopus_import: 1
status: public
title: Capture of activity-induced ultrastructural changes at synapses by high-pressure
freezing of brain tissue
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 9
year: '2014'
...
---
_id: '2230'
abstract:
- lang: eng
text: Intracellular electrophysiological recordings provide crucial insights into
elementary neuronal signals such as action potentials and synaptic currents. Analyzing
and interpreting these signals is essential for a quantitative understanding of
neuronal information processing, and requires both fast data visualization and
ready access to complex analysis routines. To achieve this goal, we have developed
Stimfit, a free software package for cellular neurophysiology with a Python scripting
interface and a built-in Python shell. The program supports most standard file
formats for cellular neurophysiology and other biomedical signals through the
Biosig library. To quantify and interpret the activity of single neurons and communication
between neurons, the program includes algorithms to characterize the kinetics
of presynaptic action potentials and postsynaptic currents, estimate latencies
between pre- and postsynaptic events, and detect spontaneously occurring events.
We validate and benchmark these algorithms, give estimation errors, and provide
sample use cases, showing that Stimfit represents an efficient, accessible and
extensible way to accurately analyze and interpret neuronal signals.
article_number: '16'
author:
- first_name: José
full_name: Guzmán, José
id: 30CC5506-F248-11E8-B48F-1D18A9856A87
last_name: Guzmán
- first_name: Alois
full_name: Schlögl, Alois
id: 45BF87EE-F248-11E8-B48F-1D18A9856A87
last_name: Schlögl
orcid: 0000-0002-5621-8100
- first_name: Christoph
full_name: Schmidt Hieber, Christoph
last_name: Schmidt Hieber
citation:
ama: 'Guzmán J, Schlögl A, Schmidt Hieber C. Stimfit: Quantifying electrophysiological
data with Python. Frontiers in Neuroinformatics. 2014;8(FEB). doi:10.3389/fninf.2014.00016'
apa: 'Guzmán, J., Schlögl, A., & Schmidt Hieber, C. (2014). Stimfit: Quantifying
electrophysiological data with Python. Frontiers in Neuroinformatics. Frontiers
Research Foundation. https://doi.org/10.3389/fninf.2014.00016'
chicago: 'Guzmán, José, Alois Schlögl, and Christoph Schmidt Hieber. “Stimfit: Quantifying
Electrophysiological Data with Python.” Frontiers in Neuroinformatics.
Frontiers Research Foundation, 2014. https://doi.org/10.3389/fninf.2014.00016.'
ieee: 'J. Guzmán, A. Schlögl, and C. Schmidt Hieber, “Stimfit: Quantifying electrophysiological
data with Python,” Frontiers in Neuroinformatics, vol. 8, no. FEB. Frontiers
Research Foundation, 2014.'
ista: 'Guzmán J, Schlögl A, Schmidt Hieber C. 2014. Stimfit: Quantifying electrophysiological
data with Python. Frontiers in Neuroinformatics. 8(FEB), 16.'
mla: 'Guzmán, José, et al. “Stimfit: Quantifying Electrophysiological Data with
Python.” Frontiers in Neuroinformatics, vol. 8, no. FEB, 16, Frontiers
Research Foundation, 2014, doi:10.3389/fninf.2014.00016.'
short: J. Guzmán, A. Schlögl, C. Schmidt Hieber, Frontiers in Neuroinformatics 8
(2014).
date_created: 2018-12-11T11:56:27Z
date_published: 2014-02-21T00:00:00Z
date_updated: 2021-01-12T06:56:09Z
day: '21'
ddc:
- '570'
department:
- _id: ScienComp
- _id: PeJo
doi: 10.3389/fninf.2014.00016
file:
- access_level: open_access
checksum: eeca00bba7232ff7d27db83321f6ea30
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:12:17Z
date_updated: 2020-07-14T12:45:34Z
file_id: '4935'
file_name: IST-2016-425-v1+1_fninf-08-00016.pdf
file_size: 2883372
relation: main_file
file_date_updated: 2020-07-14T12:45:34Z
has_accepted_license: '1'
intvolume: ' 8'
issue: FEB
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
publication: Frontiers in Neuroinformatics
publication_identifier:
issn:
- '16625196'
publication_status: published
publisher: Frontiers Research Foundation
publist_id: '4731'
pubrep_id: '425'
quality_controlled: '1'
scopus_import: 1
status: public
title: 'Stimfit: Quantifying electrophysiological data with Python'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 8
year: '2014'
...
---
_id: '2228'
abstract:
- lang: eng
text: Fast-spiking, parvalbumin-expressing GABAergic interneurons, a large proportion
of which are basket cells (BCs), have a key role in feedforward and feedback inhibition,
gamma oscillations and complex information processing. For these functions, fast
propagation of action potentials (APs) from the soma to the presynaptic terminals
is important. However, the functional properties of interneuron axons remain elusive.
We examined interneuron axons by confocally targeted subcellular patch-clamp recording
in rat hippocampal slices. APs were initiated in the proximal axon ∼20 μm from
the soma and propagated to the distal axon with high reliability and speed. Subcellular
mapping revealed a stepwise increase of Na^+ conductance density from the soma
to the proximal axon, followed by a further gradual increase in the distal axon.
Active cable modeling and experiments with partial channel block revealed that
low axonal Na^+ conductance density was sufficient for reliability, but high Na^+
density was necessary for both speed of propagation and fast-spiking AP phenotype.
Our results suggest that a supercritical density of Na^+ channels compensates
for the morphological properties of interneuron axons (small segmental diameter,
extensive branching and high bouton density), ensuring fast AP propagation and
high-frequency repetitive firing.
author:
- first_name: Hua
full_name: Hu, Hua
id: 4AC0145C-F248-11E8-B48F-1D18A9856A87
last_name: Hu
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Hu H, Jonas PM. A supercritical density of Na^+ channels ensures fast signaling
in GABAergic interneuron axons. Nature Neuroscience. 2014;17(5):686-693.
doi:10.1038/nn.3678
apa: Hu, H., & Jonas, P. M. (2014). A supercritical density of Na^+ channels
ensures fast signaling in GABAergic interneuron axons. Nature Neuroscience.
Nature Publishing Group. https://doi.org/10.1038/nn.3678
chicago: Hu, Hua, and Peter M Jonas. “A Supercritical Density of Na^+ Channels Ensures
Fast Signaling in GABAergic Interneuron Axons.” Nature Neuroscience. Nature
Publishing Group, 2014. https://doi.org/10.1038/nn.3678.
ieee: H. Hu and P. M. Jonas, “A supercritical density of Na^+ channels ensures fast
signaling in GABAergic interneuron axons,” Nature Neuroscience, vol. 17,
no. 5. Nature Publishing Group, pp. 686–693, 2014.
ista: Hu H, Jonas PM. 2014. A supercritical density of Na^+ channels ensures fast
signaling in GABAergic interneuron axons. Nature Neuroscience. 17(5), 686–693.
mla: Hu, Hua, and Peter M. Jonas. “A Supercritical Density of Na^+ Channels Ensures
Fast Signaling in GABAergic Interneuron Axons.” Nature Neuroscience, vol.
17, no. 5, Nature Publishing Group, 2014, pp. 686–93, doi:10.1038/nn.3678.
short: H. Hu, P.M. Jonas, Nature Neuroscience 17 (2014) 686–693.
date_created: 2018-12-11T11:56:26Z
date_published: 2014-03-23T00:00:00Z
date_updated: 2021-01-12T06:56:08Z
day: '23'
department:
- _id: PeJo
doi: 10.1038/nn.3678
ec_funded: 1
intvolume: ' 17'
issue: '5'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4286295/
month: '03'
oa: 1
oa_version: Submitted Version
page: 686-693
project:
- _id: 25C0F108-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '268548'
name: Nanophysiology of fast-spiking, parvalbumin-expressing GABAergic interneurons
- _id: 25C26B1E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P24909-B24
name: Mechanisms of transmitter release at GABAergic synapses
publication: Nature Neuroscience
publication_identifier:
issn:
- '10976256'
publication_status: published
publisher: Nature Publishing Group
publist_id: '4733'
quality_controlled: '1'
scopus_import: 1
status: public
title: A supercritical density of Na^+ channels ensures fast signaling in GABAergic
interneuron axons
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 17
year: '2014'
...
---
_id: '2229'
abstract:
- lang: eng
text: The distance between Ca^2+ channels and release sensors determines the speed
and efficacy of synaptic transmission. Tight "nanodomain" channel-sensor
coupling initiates transmitter release at synapses in the mature brain, whereas
loose "microdomain" coupling appears restricted to early developmental
stages. To probe the coupling configuration at a plastic synapse in the mature
central nervous system, we performed paired recordings between mossy fiber terminals
and CA3 pyramidal neurons in rat hippocampus. Millimolar concentrations of both
the fast Ca^2+ chelator BAPTA [1,2-bis(2-aminophenoxy)ethane- N,N, N′,N′-tetraacetic
acid] and the slow chelator EGTA efficiently suppressed transmitter release, indicating
loose coupling between Ca^2+ channels and release sensors. Loose coupling enabled
the control of initial release probability by fast endogenous Ca^2+ buffers and
the generation of facilitation by buffer saturation. Thus, loose coupling provides
the molecular framework for presynaptic plasticity.
author:
- first_name: Nicholas
full_name: Vyleta, Nicholas
id: 36C4978E-F248-11E8-B48F-1D18A9856A87
last_name: Vyleta
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Vyleta N, Jonas PM. Loose coupling between Ca^2+ channels and release sensors
at a plastic hippocampal synapse. Science. 2014;343(6171):665-670. doi:10.1126/science.1244811
apa: Vyleta, N., & Jonas, P. M. (2014). Loose coupling between Ca^2+ channels
and release sensors at a plastic hippocampal synapse. Science. American
Association for the Advancement of Science. https://doi.org/10.1126/science.1244811
chicago: Vyleta, Nicholas, and Peter M Jonas. “Loose Coupling between Ca^2+ Channels
and Release Sensors at a Plastic Hippocampal Synapse.” Science. American
Association for the Advancement of Science, 2014. https://doi.org/10.1126/science.1244811.
ieee: N. Vyleta and P. M. Jonas, “Loose coupling between Ca^2+ channels and release
sensors at a plastic hippocampal synapse,” Science, vol. 343, no. 6171.
American Association for the Advancement of Science, pp. 665–670, 2014.
ista: Vyleta N, Jonas PM. 2014. Loose coupling between Ca^2+ channels and release
sensors at a plastic hippocampal synapse. Science. 343(6171), 665–670.
mla: Vyleta, Nicholas, and Peter M. Jonas. “Loose Coupling between Ca^2+ Channels
and Release Sensors at a Plastic Hippocampal Synapse.” Science, vol. 343,
no. 6171, American Association for the Advancement of Science, 2014, pp. 665–70,
doi:10.1126/science.1244811.
short: N. Vyleta, P.M. Jonas, Science 343 (2014) 665–670.
date_created: 2018-12-11T11:56:27Z
date_published: 2014-02-01T00:00:00Z
date_updated: 2021-01-12T06:56:09Z
day: '01'
department:
- _id: PeJo
doi: 10.1126/science.1244811
ec_funded: 1
intvolume: ' 343'
issue: '6171'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3617475/
month: '02'
oa: 1
oa_version: Submitted Version
page: 665 - 670
project:
- _id: 25C26B1E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P24909-B24
name: Mechanisms of transmitter release at GABAergic synapses
- _id: 25C0F108-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '268548'
name: Nanophysiology of fast-spiking, parvalbumin-expressing GABAergic interneurons
publication: Science
publication_identifier:
issn:
- '00368075'
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '4732'
quality_controlled: '1'
scopus_import: 1
status: public
title: Loose coupling between Ca^2+ channels and release sensors at a plastic hippocampal
synapse
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 343
year: '2014'
...
---
_id: '2254'
abstract:
- lang: eng
text: Theta-gamma network oscillations are thought to represent key reference signals
for information processing in neuronal ensembles, but the underlying synaptic
mechanisms remain unclear. To address this question, we performed whole-cell (WC)
patch-clamp recordings from mature hippocampal granule cells (GCs) in vivo in
the dentate gyrus of anesthetized and awake rats. GCs in vivo fired action potentials
at low frequency, consistent with sparse coding in the dentate gyrus. GCs were
exposed to barrages of fast AMPAR-mediated excitatory postsynaptic currents (EPSCs),
primarily relayed from the entorhinal cortex, and inhibitory postsynaptic currents
(IPSCs), presumably generated by local interneurons. EPSCs exhibited coherence
with the field potential predominantly in the theta frequency band, whereas IPSCs
showed coherence primarily in the gamma range. Action potentials in GCs were phase
locked to network oscillations. Thus, theta-gamma-modulated synaptic currents
may provide a framework for sparse temporal coding of information in the dentate
gyrus.
author:
- first_name: Alejandro
full_name: Pernia-Andrade, Alejandro
id: 36963E98-F248-11E8-B48F-1D18A9856A87
last_name: Pernia-Andrade
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Pernia-Andrade A, Jonas PM. Theta-gamma-modulated synaptic currents in hippocampal
granule cells in vivo define a mechanism for network oscillations. Neuron.
2014;81(1):140-152. doi:10.1016/j.neuron.2013.09.046
apa: Pernia-Andrade, A., & Jonas, P. M. (2014). Theta-gamma-modulated synaptic
currents in hippocampal granule cells in vivo define a mechanism for network oscillations.
Neuron. Elsevier. https://doi.org/10.1016/j.neuron.2013.09.046
chicago: Pernia-Andrade, Alejandro, and Peter M Jonas. “Theta-Gamma-Modulated Synaptic
Currents in Hippocampal Granule Cells in Vivo Define a Mechanism for Network Oscillations.”
Neuron. Elsevier, 2014. https://doi.org/10.1016/j.neuron.2013.09.046.
ieee: A. Pernia-Andrade and P. M. Jonas, “Theta-gamma-modulated synaptic currents
in hippocampal granule cells in vivo define a mechanism for network oscillations,”
Neuron, vol. 81, no. 1. Elsevier, pp. 140–152, 2014.
ista: Pernia-Andrade A, Jonas PM. 2014. Theta-gamma-modulated synaptic currents
in hippocampal granule cells in vivo define a mechanism for network oscillations.
Neuron. 81(1), 140–152.
mla: Pernia-Andrade, Alejandro, and Peter M. Jonas. “Theta-Gamma-Modulated Synaptic
Currents in Hippocampal Granule Cells in Vivo Define a Mechanism for Network Oscillations.”
Neuron, vol. 81, no. 1, Elsevier, 2014, pp. 140–52, doi:10.1016/j.neuron.2013.09.046.
short: A. Pernia-Andrade, P.M. Jonas, Neuron 81 (2014) 140–152.
date_created: 2018-12-11T11:56:35Z
date_published: 2014-01-08T00:00:00Z
date_updated: 2021-01-12T06:56:19Z
day: '08'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1016/j.neuron.2013.09.046
ec_funded: 1
file:
- access_level: open_access
checksum: 438547cfcd9045a22f065f2019f07849
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:09:48Z
date_updated: 2020-07-14T12:45:35Z
file_id: '4773'
file_name: IST-2016-422-v1+1_1-s2.0-S0896627313009227-main.pdf
file_size: 4373072
relation: main_file
file_date_updated: 2020-07-14T12:45:35Z
has_accepted_license: '1'
intvolume: ' 81'
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: 140 - 152
project:
- _id: 25C0F108-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '268548'
name: Nanophysiology of fast-spiking, parvalbumin-expressing GABAergic interneurons
- _id: 25C26B1E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P24909-B24
name: Mechanisms of transmitter release at GABAergic synapses
publication: Neuron
publication_identifier:
issn:
- '08966273'
publication_status: published
publisher: Elsevier
publist_id: '4692'
pubrep_id: '422'
quality_controlled: '1'
scopus_import: 1
status: public
title: Theta-gamma-modulated synaptic currents in hippocampal granule cells in vivo
define a mechanism for network oscillations
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 81
year: '2014'
...
---
_id: '2285'
abstract:
- lang: eng
text: GABAergic inhibitory interneurons control fundamental aspects of neuronal
network function. Their functional roles are assumed to be defined by the identity
of their input synapses, the architecture of their dendritic tree, the passive
and active membrane properties and finally the nature of their postsynaptic targets.
Indeed, interneurons display a high degree of morphological and physiological
heterogeneity. However, whether their morphological and physiological characteristics
are correlated and whether interneuron diversity can be described by a continuum
of GABAergic cell types or by distinct classes has remained unclear. Here we perform
a detailed morphological and physiological characterization of GABAergic cells
in the dentate gyrus, the input region of the hippocampus. To achieve an unbiased
and efficient sampling and classification we used knock-in mice expressing the
enhanced green fluorescent protein (eGFP) in glutamate decarboxylase 67 (GAD67)-positive
neurons and performed cluster analysis. We identified five interneuron classes,
each of them characterized by a distinct set of anatomical and physiological parameters.
Cross-correlation analysis further revealed a direct relation between morphological
and physiological properties indicating that dentate gyrus interneurons fall into
functionally distinct classes which may differentially control neuronal network
activity.
acknowledgement: 'Funded by Deutsche Forschungsgemeinschaft. Grant Numbers: SFB 505,
SFB 780, BA1582/2-1 Excellence Initiative of the German Research Foundation (Spemann
Graduate School). Grant Number: GSC-4 Lichtenberg Professorship-Award (VW-Foundation);
Schram-Foundation; Excellence Initiative Brain Links-Brain Tools. The authors thank
Drs. Jonas-Frederic Sauer and Claudio Elgueta for critically reading the manuscript.
They also thank Karin Winterhalter, Margit Northemann and Ulrich Nöller for technical
assistance.'
author:
- first_name: Jonas
full_name: Hosp, Jonas
last_name: Hosp
- first_name: Michael
full_name: Strüber, Michael
last_name: Strüber
- first_name: Yuchio
full_name: Yanagawa, Yuchio
last_name: Yanagawa
- first_name: Kunihiko
full_name: Obata, Kunihiko
last_name: Obata
- first_name: Imre
full_name: Vida, Imre
last_name: Vida
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: Marlene
full_name: Bartos, Marlene
last_name: Bartos
citation:
ama: Hosp J, Strüber M, Yanagawa Y, et al. Morpho-physiological criteria divide
dentate gyrus interneurons into classes. Hippocampus. 2014;23(2):189-203.
doi:10.1002/hipo.22214
apa: Hosp, J., Strüber, M., Yanagawa, Y., Obata, K., Vida, I., Jonas, P. M., &
Bartos, M. (2014). Morpho-physiological criteria divide dentate gyrus interneurons
into classes. Hippocampus. Wiley-Blackwell. https://doi.org/10.1002/hipo.22214
chicago: Hosp, Jonas, Michael Strüber, Yuchio Yanagawa, Kunihiko Obata, Imre Vida,
Peter M Jonas, and Marlene Bartos. “Morpho-Physiological Criteria Divide Dentate
Gyrus Interneurons into Classes.” Hippocampus. Wiley-Blackwell, 2014. https://doi.org/10.1002/hipo.22214.
ieee: J. Hosp et al., “Morpho-physiological criteria divide dentate gyrus
interneurons into classes,” Hippocampus, vol. 23, no. 2. Wiley-Blackwell,
pp. 189–203, 2014.
ista: Hosp J, Strüber M, Yanagawa Y, Obata K, Vida I, Jonas PM, Bartos M. 2014.
Morpho-physiological criteria divide dentate gyrus interneurons into classes.
Hippocampus. 23(2), 189–203.
mla: Hosp, Jonas, et al. “Morpho-Physiological Criteria Divide Dentate Gyrus Interneurons
into Classes.” Hippocampus, vol. 23, no. 2, Wiley-Blackwell, 2014, pp.
189–203, doi:10.1002/hipo.22214.
short: J. Hosp, M. Strüber, Y. Yanagawa, K. Obata, I. Vida, P.M. Jonas, M. Bartos,
Hippocampus 23 (2014) 189–203.
date_created: 2018-12-11T11:56:46Z
date_published: 2014-02-01T00:00:00Z
date_updated: 2021-01-12T06:56:32Z
day: '01'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1002/hipo.22214
file:
- access_level: open_access
checksum: ff6bc75a79dbc985a2e31b79253e6444
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:15:54Z
date_updated: 2020-07-14T12:45:37Z
file_id: '5178'
file_name: IST-2016-461-v1+1_Hosp_et_al-2014-Hippocampus.pdf
file_size: 801589
relation: main_file
file_date_updated: 2020-07-14T12:45:37Z
has_accepted_license: '1'
intvolume: ' 23'
issue: '2'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: 189 - 203
publication: Hippocampus
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4646'
pubrep_id: '461'
quality_controlled: '1'
scopus_import: 1
status: public
title: Morpho-physiological criteria divide dentate gyrus interneurons into classes
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: journal_article
user_id: 3FFCCD3A-F248-11E8-B48F-1D18A9856A87
volume: 23
year: '2014'
...
---
_id: '10396'
abstract:
- lang: eng
text: Stimfit is a free cross-platform software package for viewing and analyzing
electrophysiological data. It supports most standard file types for cellular neurophysiology
and other biomedical formats. Its analysis algorithms have been used and validated
in several experimental laboratories. Its embedded Python scripting interface
makes Stimfit highly extensible and customizable.
article_number: '000010151520134181'
article_processing_charge: No
article_type: original
author:
- first_name: Alois
full_name: Schlögl, Alois
id: 45BF87EE-F248-11E8-B48F-1D18A9856A87
last_name: Schlögl
orcid: 0000-0002-5621-8100
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: C.
full_name: Schmidt-Hieber, C.
last_name: Schmidt-Hieber
- first_name: S. J.
full_name: Guzman, S. J.
last_name: Guzman
citation:
ama: 'Schlögl A, Jonas PM, Schmidt-Hieber C, Guzman SJ. Stimfit: A fast visualization
and analysis environment for cellular neurophysiology. Biomedical Engineering
/ Biomedizinische Technik. 2013;58(SI-1-Track-G). doi:10.1515/bmt-2013-4181'
apa: 'Schlögl, A., Jonas, P. M., Schmidt-Hieber, C., & Guzman, S. J. (2013).
Stimfit: A fast visualization and analysis environment for cellular neurophysiology.
Biomedical Engineering / Biomedizinische Technik. Graz, Austria: De Gruyter.
https://doi.org/10.1515/bmt-2013-4181'
chicago: 'Schlögl, Alois, Peter M Jonas, C. Schmidt-Hieber, and S. J. Guzman. “Stimfit:
A Fast Visualization and Analysis Environment for Cellular Neurophysiology.” Biomedical
Engineering / Biomedizinische Technik. De Gruyter, 2013. https://doi.org/10.1515/bmt-2013-4181.'
ieee: 'A. Schlögl, P. M. Jonas, C. Schmidt-Hieber, and S. J. Guzman, “Stimfit: A
fast visualization and analysis environment for cellular neurophysiology,” Biomedical
Engineering / Biomedizinische Technik, vol. 58, no. SI-1-Track-G. De Gruyter,
2013.'
ista: 'Schlögl A, Jonas PM, Schmidt-Hieber C, Guzman SJ. 2013. Stimfit: A fast visualization
and analysis environment for cellular neurophysiology. Biomedical Engineering
/ Biomedizinische Technik. 58(SI-1-Track-G), 000010151520134181.'
mla: 'Schlögl, Alois, et al. “Stimfit: A Fast Visualization and Analysis Environment
for Cellular Neurophysiology.” Biomedical Engineering / Biomedizinische Technik,
vol. 58, no. SI-1-Track-G, 000010151520134181, De Gruyter, 2013, doi:10.1515/bmt-2013-4181.'
short: A. Schlögl, P.M. Jonas, C. Schmidt-Hieber, S.J. Guzman, Biomedical Engineering
/ Biomedizinische Technik 58 (2013).
conference:
end_date: 2013-09-21
location: Graz, Austria
name: 'BMT: Biomedizinische Technik '
start_date: 2013-09-19
date_created: 2021-12-01T14:35:35Z
date_published: 2013-08-01T00:00:00Z
date_updated: 2021-12-02T12:51:12Z
day: '01'
ddc:
- '005'
- '610'
department:
- _id: PeJo
doi: 10.1515/bmt-2013-4181
external_id:
pmid:
- '24042795'
file:
- access_level: open_access
checksum: cdfc5339b530a25d6079f7223f0b1f16
content_type: application/pdf
creator: schloegl
date_created: 2021-12-01T14:38:08Z
date_updated: 2021-12-01T14:38:08Z
file_id: '10397'
file_name: Schloegl_Abstract-BMT2013.pdf
file_size: 149825
relation: main_file
success: 1
file_date_updated: 2021-12-01T14:38:08Z
has_accepted_license: '1'
intvolume: ' 58'
issue: SI-1-Track-G
keyword:
- biomedical engineering
- data analysis
- free software
language:
- iso: eng
month: '08'
oa: 1
oa_version: Submitted Version
pmid: 1
publication: Biomedical Engineering / Biomedizinische Technik
publication_identifier:
eissn:
- 1862-278X
issn:
- 0013-5585
publication_status: published
publisher: De Gruyter
quality_controlled: '1'
status: public
title: 'Stimfit: A fast visualization and analysis environment for cellular neurophysiology'
type: journal_article
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
volume: 58
year: '2013'
...
---
_id: '2954'
abstract:
- lang: eng
text: Spontaneous postsynaptic currents (PSCs) provide key information about the
mechanisms of synaptic transmission and the activity modes of neuronal networks.
However, detecting spontaneous PSCs in vitro and in vivo has been challenging,
because of the small amplitude, the variable kinetics, and the undefined time
of generation of these events. Here, we describe a, to our knowledge, new method
for detecting spontaneous synaptic events by deconvolution, using a template that
approximates the average time course of spontaneous PSCs. A recorded PSC trace
is deconvolved from the template, resulting in a series of delta-like functions.
The maxima of these delta-like events are reliably detected, revealing the precise
onset times of the spontaneous PSCs. Among all detection methods, the deconvolution-based
method has a unique temporal resolution, allowing the detection of individual
events in high-frequency bursts. Furthermore, the deconvolution-based method has
a high amplitude resolution, because deconvolution can substantially increase
the signal/noise ratio. When tested against previously published methods using
experimental data, the deconvolution-based method was superior for spontaneous
PSCs recorded in vivo. Using the high-resolution deconvolution-based detection
algorithm, we show that the frequency of spontaneous excitatory postsynaptic currents
in dentate gyrus granule cells is 4.5 times higher in vivo than in vitro.
acknowledgement: "This work was supported by the Deutsche Forschungsgemeinschaft (TR3/B10)
and a European Research Council Advanced grant to P.J.\r\nWe thank H. Hu, S. J.
Guzman, and C. Schmidt-Hieber for critically reading the manuscript, I. Koeva and
F. Marr for technical support, and E. Kramberger for editorial assistance.\r\n"
author:
- first_name: Alejandro
full_name: Pernia-Andrade, Alejandro
id: 36963E98-F248-11E8-B48F-1D18A9856A87
last_name: Pernia-Andrade
- first_name: Sarit
full_name: Goswami, Sarit
id: 3A578F32-F248-11E8-B48F-1D18A9856A87
last_name: Goswami
- first_name: Yvonne
full_name: Stickler, Yvonne
id: 63B76600-E9CC-11E9-9B5F-82450873F7A1
last_name: Stickler
- first_name: Ulrich
full_name: Fröbe, Ulrich
last_name: Fröbe
- first_name: Alois
full_name: Schlögl, Alois
id: 45BF87EE-F248-11E8-B48F-1D18A9856A87
last_name: Schlögl
orcid: 0000-0002-5621-8100
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Pernia-Andrade A, Goswami S, Stickler Y, Fröbe U, Schlögl A, Jonas PM. A deconvolution
based method with high sensitivity and temporal resolution for detection of spontaneous
synaptic currents in vitro and in vivo. Biophysical Journal. 2012;103(7):1429-1439.
doi:10.1016/j.bpj.2012.08.039
apa: Pernia-Andrade, A., Goswami, S., Stickler, Y., Fröbe, U., Schlögl, A., &
Jonas, P. M. (2012). A deconvolution based method with high sensitivity and temporal
resolution for detection of spontaneous synaptic currents in vitro and in vivo.
Biophysical Journal. Biophysical. https://doi.org/10.1016/j.bpj.2012.08.039
chicago: Pernia-Andrade, Alejandro, Sarit Goswami, Yvonne Stickler, Ulrich Fröbe,
Alois Schlögl, and Peter M Jonas. “A Deconvolution Based Method with High Sensitivity
and Temporal Resolution for Detection of Spontaneous Synaptic Currents in Vitro
and in Vivo.” Biophysical Journal. Biophysical, 2012. https://doi.org/10.1016/j.bpj.2012.08.039.
ieee: A. Pernia-Andrade, S. Goswami, Y. Stickler, U. Fröbe, A. Schlögl, and P. M.
Jonas, “A deconvolution based method with high sensitivity and temporal resolution
for detection of spontaneous synaptic currents in vitro and in vivo,” Biophysical
Journal, vol. 103, no. 7. Biophysical, pp. 1429–1439, 2012.
ista: Pernia-Andrade A, Goswami S, Stickler Y, Fröbe U, Schlögl A, Jonas PM. 2012.
A deconvolution based method with high sensitivity and temporal resolution for
detection of spontaneous synaptic currents in vitro and in vivo. Biophysical Journal.
103(7), 1429–1439.
mla: Pernia-Andrade, Alejandro, et al. “A Deconvolution Based Method with High Sensitivity
and Temporal Resolution for Detection of Spontaneous Synaptic Currents in Vitro
and in Vivo.” Biophysical Journal, vol. 103, no. 7, Biophysical, 2012,
pp. 1429–39, doi:10.1016/j.bpj.2012.08.039.
short: A. Pernia-Andrade, S. Goswami, Y. Stickler, U. Fröbe, A. Schlögl, P.M. Jonas,
Biophysical Journal 103 (2012) 1429–1439.
date_created: 2018-12-11T12:00:32Z
date_published: 2012-10-03T00:00:00Z
date_updated: 2021-01-12T07:40:01Z
day: '03'
department:
- _id: PeJo
- _id: ScienComp
doi: 10.1016/j.bpj.2012.08.039
external_id:
pmid:
- '23062335'
intvolume: ' 103'
issue: '7'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3471482/
month: '10'
oa: 1
oa_version: Submitted Version
page: 1429 - 1439
pmid: 1
project:
- _id: 25BDE9A4-B435-11E9-9278-68D0E5697425
grant_number: SFB-TR3-TP10B
name: Glutamaterge synaptische Übertragung und Plastizität in hippocampalen Mikroschaltkreisen
publication: Biophysical Journal
publication_status: published
publisher: Biophysical
publist_id: '3774'
quality_controlled: '1'
scopus_import: 1
status: public
title: A deconvolution based method with high sensitivity and temporal resolution
for detection of spontaneous synaptic currents in vitro and in vivo
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 103
year: '2012'
...
---
_id: '2969'
abstract:
- lang: eng
text: "The coupling between presynaptic Ca^(2+) channels and Ca^(2+) sensors of
exocytosis is a key determinant of synaptic transmission. Evoked release from
parvalbumin (PV)-expressing interneurons is triggered by nanodomain coupling of
P/Q-type Ca^(2+) channels, whereas release from cholecystokinin (CCK)-containing
interneurons is generated by microdomain coupling of N-type channels. Nanodomain
coupling has several functional advantages, including speed and efficacy of transmission.
One potential disadvantage is that stochastic\r\nopening of presynaptic Ca^(2+)
channels may trigger spontaneous transmitter release. We addressed this possibility
in rat hippocampal\r\ngranule cells, which receive converging inputs from different
inhibitory sources. Both reduction of extracellular Ca^(2+) concentration and
the unselective Ca^(2+) channel blocker Cd^(2+) reduced the frequency of miniature
IPSCs (mIPSCs) in granule cells by ~50%, suggesting that the opening of presynaptic
Ca^(2+) channels contributes to spontaneous release. Application of the selective
P/Q-type Ca^(2+) channel blocker\r\nω-agatoxin IVa had no detectable effects,
whereas both the N-type blocker ω-conotoxin GVIa and the L-type blocker nimodipine
reduced\r\nmIPSC frequency. Furthermore, both the fast Ca^(2+) chelator BAPTA-AM
and the slow chelator EGTA-AM reduced the mIPSC frequency,\r\nsuggesting that
Ca^(2+)-dependent spontaneous release is triggered by microdomain rather than
nanodomain coupling. The CB_(1) receptor\r\nagonist WIN 55212-2 also decreased
spontaneous release; this effect was occluded by prior application of ω-conotoxin
GVIa, suggesting that a major fraction of Ca^(2+)-dependent spontaneous release
was generated at the terminals of CCK-expressing interneurons. Tonic inhibition
generated by spontaneous opening of presynaptic N- and L-type Ca^(2+) channels
may be important for hippocampal information processing.\r\n"
acknowledgement: This work was supported by grants from the Deutsche Forschungsgemeinschaft
(TR 3/B10, Leibniz program, GSC-4 Spemann Graduate School) and the European Union
(European Research Council Advanced Grant).
author:
- first_name: Sarit
full_name: Goswami, Sarit
id: 3A578F32-F248-11E8-B48F-1D18A9856A87
last_name: Goswami
- first_name: Iancu
full_name: Bucurenciu, Iancu
last_name: Bucurenciu
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Goswami S, Bucurenciu I, Jonas PM. Miniature IPSCs in hippocampal granule cells
are triggered by voltage-gated Ca^(2+) channels via microdomain coupling. Journal
of Neuroscience. 2012;32(41):14294-14304. doi:10.1523/JNEUROSCI.6104-11.2012
apa: Goswami, S., Bucurenciu, I., & Jonas, P. M. (2012). Miniature IPSCs in
hippocampal granule cells are triggered by voltage-gated Ca^(2+) channels via
microdomain coupling. Journal of Neuroscience. Society for Neuroscience.
https://doi.org/10.1523/JNEUROSCI.6104-11.2012
chicago: Goswami, Sarit, Iancu Bucurenciu, and Peter M Jonas. “Miniature IPSCs in
Hippocampal Granule Cells Are Triggered by Voltage-Gated Ca^(2+) Channels via
Microdomain Coupling.” Journal of Neuroscience. Society for Neuroscience,
2012. https://doi.org/10.1523/JNEUROSCI.6104-11.2012.
ieee: S. Goswami, I. Bucurenciu, and P. M. Jonas, “Miniature IPSCs in hippocampal
granule cells are triggered by voltage-gated Ca^(2+) channels via microdomain
coupling,” Journal of Neuroscience, vol. 32, no. 41. Society for Neuroscience,
pp. 14294–14304, 2012.
ista: Goswami S, Bucurenciu I, Jonas PM. 2012. Miniature IPSCs in hippocampal granule
cells are triggered by voltage-gated Ca^(2+) channels via microdomain coupling.
Journal of Neuroscience. 32(41), 14294–14304.
mla: Goswami, Sarit, et al. “Miniature IPSCs in Hippocampal Granule Cells Are Triggered
by Voltage-Gated Ca^(2+) Channels via Microdomain Coupling.” Journal of Neuroscience,
vol. 32, no. 41, Society for Neuroscience, 2012, pp. 14294–304, doi:10.1523/JNEUROSCI.6104-11.2012.
short: S. Goswami, I. Bucurenciu, P.M. Jonas, Journal of Neuroscience 32 (2012)
14294–14304.
date_created: 2018-12-11T12:00:36Z
date_published: 2012-10-10T00:00:00Z
date_updated: 2021-01-12T07:40:08Z
day: '10'
department:
- _id: PeJo
doi: 10.1523/JNEUROSCI.6104-11.2012
external_id:
pmid:
- '23055500'
intvolume: ' 32'
issue: '41'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3632771/
month: '10'
oa: 1
oa_version: Submitted Version
page: 14294 - 14304
pmid: 1
project:
- _id: 25BDE9A4-B435-11E9-9278-68D0E5697425
grant_number: SFB-TR3-TP10B
name: Glutamaterge synaptische Übertragung und Plastizität in hippocampalen Mikroschaltkreisen
publication: Journal of Neuroscience
publication_status: published
publisher: Society for Neuroscience
publist_id: '3744'
quality_controlled: '1'
scopus_import: 1
status: public
title: Miniature IPSCs in hippocampal granule cells are triggered by voltage-gated
Ca^(2+) channels via microdomain coupling
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 32
year: '2012'
...
---
_id: '3121'
abstract:
- lang: eng
text: Voltage-activated Ca(2+) channels (VACCs) mediate Ca(2+) influx to trigger
action potential-evoked neurotransmitter release, but the mechanism by which Ca(2+)
regulates spontaneous transmission is unclear. We found that VACCs are the major
physiological triggers for spontaneous release at mouse neocortical inhibitory
synapses. Moreover, despite the absence of a synchronizing action potential, we
found that spontaneous fusion of a GABA-containing vesicle required the activation
of multiple tightly coupled VACCs of variable type.
acknowledgement: "The work was supported by the US National Institutes of Health (DA027110
and GM097433) and OCTRI. C.W. and N.P.V. were supported by a grant from the National
Heart, Lung, and Blood Institute (T32HL033808).\r\nWe thank M. Andresen and K. Khodakhah
for helpful comments. "
author:
- first_name: Courtney
full_name: Williams, Courtney
last_name: Williams
- first_name: Wenyan
full_name: Chen, Wenyan
last_name: Chen
- first_name: Chia
full_name: Lee, Chia
last_name: Lee
- first_name: Daniel
full_name: Yaeger, Daniel
last_name: Yaeger
- first_name: Nicholas
full_name: Vyleta, Nicholas
id: 36C4978E-F248-11E8-B48F-1D18A9856A87
last_name: Vyleta
- first_name: Stephen
full_name: Smith, Stephen
last_name: Smith
citation:
ama: Williams C, Chen W, Lee C, Yaeger D, Vyleta N, Smith S. Coactivation of multiple
tightly coupled calcium channels triggers spontaneous release of GABA. Nature
Neuroscience. 2012;15(9):1195-1197. doi:10.1038/nn.3162
apa: Williams, C., Chen, W., Lee, C., Yaeger, D., Vyleta, N., & Smith, S. (2012).
Coactivation of multiple tightly coupled calcium channels triggers spontaneous
release of GABA. Nature Neuroscience. Nature Publishing Group. https://doi.org/10.1038/nn.3162
chicago: Williams, Courtney, Wenyan Chen, Chia Lee, Daniel Yaeger, Nicholas Vyleta,
and Stephen Smith. “Coactivation of Multiple Tightly Coupled Calcium Channels
Triggers Spontaneous Release of GABA.” Nature Neuroscience. Nature Publishing
Group, 2012. https://doi.org/10.1038/nn.3162.
ieee: C. Williams, W. Chen, C. Lee, D. Yaeger, N. Vyleta, and S. Smith, “Coactivation
of multiple tightly coupled calcium channels triggers spontaneous release of GABA,”
Nature Neuroscience, vol. 15, no. 9. Nature Publishing Group, pp. 1195–1197,
2012.
ista: Williams C, Chen W, Lee C, Yaeger D, Vyleta N, Smith S. 2012. Coactivation
of multiple tightly coupled calcium channels triggers spontaneous release of GABA.
Nature Neuroscience. 15(9), 1195–1197.
mla: Williams, Courtney, et al. “Coactivation of Multiple Tightly Coupled Calcium
Channels Triggers Spontaneous Release of GABA.” Nature Neuroscience, vol.
15, no. 9, Nature Publishing Group, 2012, pp. 1195–97, doi:10.1038/nn.3162.
short: C. Williams, W. Chen, C. Lee, D. Yaeger, N. Vyleta, S. Smith, Nature Neuroscience
15 (2012) 1195–1197.
date_created: 2018-12-11T12:01:30Z
date_published: 2012-09-01T00:00:00Z
date_updated: 2021-01-12T07:41:12Z
day: '01'
department:
- _id: PeJo
doi: 10.1038/nn.3162
external_id:
pmid:
- '22842148'
intvolume: ' 15'
issue: '9'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3431448/
month: '09'
oa: 1
oa_version: Submitted Version
page: 1195 - 1197
pmid: 1
publication: Nature Neuroscience
publication_status: published
publisher: Nature Publishing Group
publist_id: '3578'
quality_controlled: '1'
scopus_import: 1
status: public
title: Coactivation of multiple tightly coupled calcium channels triggers spontaneous
release of GABA
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 15
year: '2012'
...
---
_id: '3317'
abstract:
- lang: eng
text: The physical distance between presynaptic Ca2+ channels and the Ca2+ sensors
that trigger exocytosis of neurotransmitter-containing vesicles is a key determinant
of the signalling properties of synapses in the nervous system. Recent functional
analysis indicates that in some fast central synapses, transmitter release is
triggered by a small number of Ca2+ channels that are coupled to Ca2+ sensors
at the nanometre scale. Molecular analysis suggests that this tight coupling is
generated by protein–protein interactions involving Ca2+ channels, Ca2+ sensors
and various other synaptic proteins. Nanodomain coupling has several functional
advantages, as it increases the efficacy, speed and energy efficiency of synaptic
transmission.
acknowledgement: "Work of the authors was funded by grants of the Deutsche Forschungsgemeinschaft
to P.J. (grants SFB 780/A5, TR 3/B10 and the Leibniz programme), a European Research
Council Advanced grant to P.J. and a Swiss National Foundation fellowship to E.E.\r\nWe
thank D. Tsien and E. Neher for their comments on this Review, J. Guzmán and A.
Pernía-Andrade for reading earlier versions and E. Kramberger for perfect editorial
support. We apologize that owing to space constraints, not all relevant papers could
be cited.\r\n"
author:
- first_name: Emmanuel
full_name: Eggermann, Emmanuel
id: 34DACA34-E9AE-11E9-849C-D35BD8ADC20C
last_name: Eggermann
- first_name: Iancu
full_name: Bucurenciu, Iancu
id: 4BD1D872-E9AE-11E9-9EE9-8BF4597A9E2A
last_name: Bucurenciu
- first_name: Sarit
full_name: Goswami, Sarit
id: 3A578F32-F248-11E8-B48F-1D18A9856A87
last_name: Goswami
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Eggermann E, Bucurenciu I, Goswami S, Jonas PM. Nanodomain coupling between
Ca(2+) channels and sensors of exocytosis at fast mammalian synapses. Nature
Reviews Neuroscience. 2012;13(1):7-21. doi:10.1038/nrn3125
apa: Eggermann, E., Bucurenciu, I., Goswami, S., & Jonas, P. M. (2012). Nanodomain
coupling between Ca(2+) channels and sensors of exocytosis at fast mammalian synapses.
Nature Reviews Neuroscience. Nature Publishing Group. https://doi.org/10.1038/nrn3125
chicago: Eggermann, Emmanuel, Iancu Bucurenciu, Sarit Goswami, and Peter M Jonas.
“Nanodomain Coupling between Ca(2+) Channels and Sensors of Exocytosis at Fast
Mammalian Synapses.” Nature Reviews Neuroscience. Nature Publishing Group,
2012. https://doi.org/10.1038/nrn3125.
ieee: E. Eggermann, I. Bucurenciu, S. Goswami, and P. M. Jonas, “Nanodomain coupling
between Ca(2+) channels and sensors of exocytosis at fast mammalian synapses,”
Nature Reviews Neuroscience, vol. 13, no. 1. Nature Publishing Group, pp.
7–21, 2012.
ista: Eggermann E, Bucurenciu I, Goswami S, Jonas PM. 2012. Nanodomain coupling
between Ca(2+) channels and sensors of exocytosis at fast mammalian synapses.
Nature Reviews Neuroscience. 13(1), 7–21.
mla: Eggermann, Emmanuel, et al. “Nanodomain Coupling between Ca(2+) Channels and
Sensors of Exocytosis at Fast Mammalian Synapses.” Nature Reviews Neuroscience,
vol. 13, no. 1, Nature Publishing Group, 2012, pp. 7–21, doi:10.1038/nrn3125.
short: E. Eggermann, I. Bucurenciu, S. Goswami, P.M. Jonas, Nature Reviews Neuroscience
13 (2012) 7–21.
date_created: 2018-12-11T12:02:38Z
date_published: 2012-01-01T00:00:00Z
date_updated: 2021-01-12T07:42:36Z
day: '01'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1038/nrn3125
file:
- access_level: open_access
checksum: 4c1c86b2f6e4e1562f5bb800b457ea9f
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:12:13Z
date_updated: 2020-07-14T12:46:07Z
file_id: '4931'
file_name: IST-2017-820-v1+1_17463_3_art_file_109404_ltmxbw.pdf
file_size: 314246
relation: main_file
- access_level: open_access
checksum: bceb2efdd49d115f4dde8486bc1be3f2
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:12:14Z
date_updated: 2020-07-14T12:46:07Z
file_id: '4932'
file_name: IST-2017-820-v1+2_17463_3_figure_109402_ltmwlp.pdf
file_size: 1840216
relation: main_file
file_date_updated: 2020-07-14T12:46:07Z
has_accepted_license: '1'
intvolume: ' 13'
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Submitted Version
page: 7 - 21
project:
- _id: 25BC64A8-B435-11E9-9278-68D0E5697425
grant_number: JO_780/A5
name: Synaptic Mechanisms of Neuronal Network Function
- _id: 25BDE9A4-B435-11E9-9278-68D0E5697425
grant_number: SFB-TR3-TP10B
name: Glutamaterge synaptische Übertragung und Plastizität in hippocampalen Mikroschaltkreisen
publication: Nature Reviews Neuroscience
publication_status: published
publisher: Nature Publishing Group
publist_id: '3322'
pubrep_id: '820'
quality_controlled: '1'
scopus_import: 1
status: public
title: Nanodomain coupling between Ca(2+) channels and sensors of exocytosis at fast
mammalian synapses
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 13
year: '2012'
...
---
_id: '493'
abstract:
- lang: eng
text: 'The BCI competition IV stands in the tradition of prior BCI competitions
that aim to provide high quality neuroscientific data for open access to the scientific
community. As experienced already in prior competitions not only scientists from
the narrow field of BCI compete, but scholars with a broad variety of backgrounds
and nationalities. They include high specialists as well as students.The goals
of all BCI competitions have always been to challenge with respect to novel paradigms
and complex data. We report on the following challenges: (1) asynchronous data,
(2) synthetic, (3) multi-class continuous data, (4) sessionto-session transfer,
(5) directionally modulated MEG, (6) finger movements recorded by ECoG. As after
past competitions, our hope is that winning entries may enhance the analysis methods
of future BCIs.'
acknowledgement: "The studies were in part or completely supported by the Bundesministerium
für Bildung und Forschung (BMBF), Fkz 01IB001A, 01GQ0850, by the German Science
Foundation (DFG, contract MU 987/3-2), by the European ICT Programme Projects FP7-224631
and 216886, the World Class University Program through the National Research Foundation
of Korea funded by the Ministry of Education, Science, and Technology (Grant R31-10008),
the US Army Research Office [W911NF-08-1-0216 (Gerwin Schalk) and W911NF-07-1-0415
(Gerwin Schalk)] and the NIH [EB006356 (Gerwin Schalk) and EB000856 (Gerwin Schalk),
the WIN-Kolleg of the Heidelberg Academy of Sciences and Humanities, German Federal
Ministry of Education and Research grants 01GQ0420, 01GQ0761, 01GQ0762, and 01GQ0830,
German Research Foundation grants 550/B5 and C6, and by a scholarship from the German
National Academic Foundation. This paper only reflects the authors’ views and funding
agencies are not liable for any use that may be made of the information contained
herein.\r\n"
article_number: '55'
author:
- first_name: Michael
full_name: Tangermann, Michael
last_name: Tangermann
- first_name: Klaus
full_name: Müller, Klaus
last_name: Müller
- first_name: Ad
full_name: Aertsen, Ad
last_name: Aertsen
- first_name: Niels
full_name: Birbaumer, Niels
last_name: Birbaumer
- first_name: Christoph
full_name: Braun, Christoph
last_name: Braun
- first_name: Clemens
full_name: Brunner, Clemens
last_name: Brunner
- first_name: Robert
full_name: Leeb, Robert
last_name: Leeb
- first_name: Carsten
full_name: Mehring, Carsten
last_name: Mehring
- first_name: Kai
full_name: Miller, Kai
last_name: Miller
- first_name: Gernot
full_name: Müller Putz, Gernot
last_name: Müller Putz
- first_name: Guido
full_name: Nolte, Guido
last_name: Nolte
- first_name: Gert
full_name: Pfurtscheller, Gert
last_name: Pfurtscheller
- first_name: Hubert
full_name: Preissl, Hubert
last_name: Preissl
- first_name: Gerwin
full_name: Schalk, Gerwin
last_name: Schalk
- first_name: Alois
full_name: Schlögl, Alois
id: 45BF87EE-F248-11E8-B48F-1D18A9856A87
last_name: Schlögl
orcid: 0000-0002-5621-8100
- first_name: Carmen
full_name: Vidaurre, Carmen
last_name: Vidaurre
- first_name: Stephan
full_name: Waldert, Stephan
last_name: Waldert
- first_name: Benjamin
full_name: Blankertz, Benjamin
last_name: Blankertz
citation:
ama: Tangermann M, Müller K, Aertsen A, et al. Review of the BCI competition IV.
Frontiers in Neuroscience. 2012;6. doi:10.3389/fnins.2012.00055
apa: Tangermann, M., Müller, K., Aertsen, A., Birbaumer, N., Braun, C., Brunner,
C., … Blankertz, B. (2012). Review of the BCI competition IV. Frontiers in
Neuroscience. Frontiers Research Foundation. https://doi.org/10.3389/fnins.2012.00055
chicago: Tangermann, Michael, Klaus Müller, Ad Aertsen, Niels Birbaumer, Christoph
Braun, Clemens Brunner, Robert Leeb, et al. “Review of the BCI Competition IV.”
Frontiers in Neuroscience. Frontiers Research Foundation, 2012. https://doi.org/10.3389/fnins.2012.00055.
ieee: M. Tangermann et al., “Review of the BCI competition IV,” Frontiers
in Neuroscience, vol. 6. Frontiers Research Foundation, 2012.
ista: Tangermann M, Müller K, Aertsen A, Birbaumer N, Braun C, Brunner C, Leeb R,
Mehring C, Miller K, Müller Putz G, Nolte G, Pfurtscheller G, Preissl H, Schalk
G, Schlögl A, Vidaurre C, Waldert S, Blankertz B. 2012. Review of the BCI competition
IV. Frontiers in Neuroscience. 6, 55.
mla: Tangermann, Michael, et al. “Review of the BCI Competition IV.” Frontiers
in Neuroscience, vol. 6, 55, Frontiers Research Foundation, 2012, doi:10.3389/fnins.2012.00055.
short: M. Tangermann, K. Müller, A. Aertsen, N. Birbaumer, C. Braun, C. Brunner,
R. Leeb, C. Mehring, K. Miller, G. Müller Putz, G. Nolte, G. Pfurtscheller, H.
Preissl, G. Schalk, A. Schlögl, C. Vidaurre, S. Waldert, B. Blankertz, Frontiers
in Neuroscience 6 (2012).
date_created: 2018-12-11T11:46:46Z
date_published: 2012-07-13T00:00:00Z
date_updated: 2021-01-12T08:01:03Z
day: '13'
ddc:
- '004'
department:
- _id: ScienComp
- _id: PeJo
doi: 10.3389/fnins.2012.00055
file:
- access_level: open_access
checksum: 195238221c4b0b0f4035f6f6c16ea17c
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:18:34Z
date_updated: 2020-07-14T12:46:35Z
file_id: '5356'
file_name: IST-2018-945-v1+1_2012_Schloegl_Review_of.pdf
file_size: 2693701
relation: main_file
file_date_updated: 2020-07-14T12:46:35Z
has_accepted_license: '1'
intvolume: ' 6'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
publication: Frontiers in Neuroscience
publication_status: published
publisher: Frontiers Research Foundation
publist_id: '7327'
pubrep_id: '945'
quality_controlled: '1'
scopus_import: 1
status: public
title: Review of the BCI competition IV
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 6
year: '2012'
...
---
_id: '3258'
abstract:
- lang: eng
text: CA3 pyramidal neurons are important for memory formation and pattern completion
in the hippocampal network. It is generally thought that proximal synapses from
the mossy fibers activate these neurons most efficiently, whereas distal inputs
from the perforant path have a weaker modulatory influence. We used confocally
targeted patch-clamp recording from dendrites and axons to map the activation
of rat CA3 pyramidal neurons at the subcellular level. Our results reveal two
distinct dendritic domains. In the proximal domain, action potentials initiated
in the axon backpropagate actively with large amplitude and fast time course.
In the distal domain, Na+ channel–mediated dendritic spikes are efficiently initiated
by waveforms mimicking synaptic events. CA3 pyramidal neuron dendrites showed
a high Na+-to-K+ conductance density ratio, providing ideal conditions for active
backpropagation and dendritic spike initiation. Dendritic spikes may enhance the
computational power of CA3 pyramidal neurons in the hippocampal network.
acknowledgement: This work was supported by the Deutsche Forschungsgemeinschaft (TR
3/B10) and the European Union (European Research Council Advanced grant to P.J.).
article_processing_charge: No
article_type: original
author:
- first_name: Sooyun
full_name: Kim, Sooyun
id: 394AB1C8-F248-11E8-B48F-1D18A9856A87
last_name: Kim
- first_name: José
full_name: Guzmán, José
id: 30CC5506-F248-11E8-B48F-1D18A9856A87
last_name: Guzmán
orcid: 0000-0003-2209-5242
- first_name: Hua
full_name: Hu, Hua
id: 4AC0145C-F248-11E8-B48F-1D18A9856A87
last_name: Hu
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Kim S, Guzmán J, Hu H, Jonas PM. Active dendrites support efficient initiation
of dendritic spikes in hippocampal CA3 pyramidal neurons. Nature Neuroscience.
2012;15(4):600-606. doi:10.1038/nn.3060
apa: Kim, S., Guzmán, J., Hu, H., & Jonas, P. M. (2012). Active dendrites support
efficient initiation of dendritic spikes in hippocampal CA3 pyramidal neurons.
Nature Neuroscience. Nature Publishing Group. https://doi.org/10.1038/nn.3060
chicago: Kim, Sooyun, José Guzmán, Hua Hu, and Peter M Jonas. “Active Dendrites
Support Efficient Initiation of Dendritic Spikes in Hippocampal CA3 Pyramidal
Neurons.” Nature Neuroscience. Nature Publishing Group, 2012. https://doi.org/10.1038/nn.3060.
ieee: S. Kim, J. Guzmán, H. Hu, and P. M. Jonas, “Active dendrites support efficient
initiation of dendritic spikes in hippocampal CA3 pyramidal neurons,” Nature
Neuroscience, vol. 15, no. 4. Nature Publishing Group, pp. 600–606, 2012.
ista: Kim S, Guzmán J, Hu H, Jonas PM. 2012. Active dendrites support efficient
initiation of dendritic spikes in hippocampal CA3 pyramidal neurons. Nature Neuroscience.
15(4), 600–606.
mla: Kim, Sooyun, et al. “Active Dendrites Support Efficient Initiation of Dendritic
Spikes in Hippocampal CA3 Pyramidal Neurons.” Nature Neuroscience, vol.
15, no. 4, Nature Publishing Group, 2012, pp. 600–06, doi:10.1038/nn.3060.
short: S. Kim, J. Guzmán, H. Hu, P.M. Jonas, Nature Neuroscience 15 (2012) 600–606.
date_created: 2018-12-11T12:02:18Z
date_published: 2012-04-01T00:00:00Z
date_updated: 2023-09-07T11:43:52Z
day: '01'
department:
- _id: PeJo
doi: 10.1038/nn.3060
external_id:
pmid:
- '22388958'
intvolume: ' 15'
issue: '4'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3617474/
month: '04'
oa: 1
oa_version: Published Version
page: 600 - 606
pmid: 1
project:
- _id: 25BDE9A4-B435-11E9-9278-68D0E5697425
grant_number: SFB-TR3-TP10B
name: Glutamaterge synaptische Übertragung und Plastizität in hippocampalen Mikroschaltkreisen
publication: Nature Neuroscience
publication_identifier:
issn:
- 1546-1726
publication_status: published
publisher: Nature Publishing Group
publist_id: '3390'
quality_controlled: '1'
related_material:
record:
- id: '2964'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Active dendrites support efficient initiation of dendritic spikes in hippocampal
CA3 pyramidal neurons
type: journal_article
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
volume: 15
year: '2012'
...
---
_id: '2964'
abstract:
- lang: eng
text: 'CA3 pyramidal neurons are important for memory formation and pattern completion
in the hippocampal network. These neurons receive multiple excitatory inputs from
numerous sources. Therefore, the rules of spatiotemporal integration of multiple
synaptic inputs and propagation of action potentials are important to understand
how CA3 neurons contribute to higher brain functions at cellular level. By using
confocally targeted patch-clamp recording techniques, we investigated the biophysical
properties of rat CA3 pyramidal neuron dendrites. We found two distinct dendritic
domains critical for action potential initiation and propagation: In the proximal
domain, action potentials initiated in the axon backpropagate actively with large
amplitude and fast time course. In the distal domain, Na+-channel mediated dendritic
spikes are efficiently evoked by local dendritic depolarization or waveforms mimicking
synaptic events. These findings can be explained by a high Na+-to-K+ conductance
density ratio of CA3 pyramidal neuron dendrites. The results challenge the prevailing
view that proximal mossy fiber inputs activate CA3 pyramidal neurons more efficiently
than distal perforant inputs by showing that the distal synapses trigger a different
form of activity represented by dendritic spikes. The high probability of dendritic
spike initiation in the distal area may enhance the computational power of CA3
pyramidal neurons in the hippocampal network. '
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Sooyun
full_name: Kim, Sooyun
id: 394AB1C8-F248-11E8-B48F-1D18A9856A87
last_name: Kim
citation:
ama: Kim S. Active properties of hippocampal CA3 pyramidal neuron dendrites. 2012.
apa: Kim, S. (2012). Active properties of hippocampal CA3 pyramidal neuron dendrites.
Institute of Science and Technology Austria.
chicago: Kim, Sooyun. “Active Properties of Hippocampal CA3 Pyramidal Neuron Dendrites.”
Institute of Science and Technology Austria, 2012.
ieee: S. Kim, “Active properties of hippocampal CA3 pyramidal neuron dendrites,”
Institute of Science and Technology Austria, 2012.
ista: Kim S. 2012. Active properties of hippocampal CA3 pyramidal neuron dendrites.
Institute of Science and Technology Austria.
mla: Kim, Sooyun. Active Properties of Hippocampal CA3 Pyramidal Neuron Dendrites.
Institute of Science and Technology Austria, 2012.
short: S. Kim, Active Properties of Hippocampal CA3 Pyramidal Neuron Dendrites,
Institute of Science and Technology Austria, 2012.
date_created: 2018-12-11T12:00:35Z
date_published: 2012-06-01T00:00:00Z
date_updated: 2023-09-07T11:43:51Z
day: '01'
degree_awarded: PhD
department:
- _id: PeJo
- _id: GradSch
language:
- iso: eng
month: '06'
oa_version: None
page: '65'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '3755'
related_material:
record:
- id: '3258'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
title: Active properties of hippocampal CA3 pyramidal neuron dendrites
type: dissertation
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
year: '2012'
...
---
_id: '3318'
abstract:
- lang: eng
text: Parvalbumin is thought to act in a manner similar to EGTA, but how a slow
Ca2+ buffer affects nanodomain-coupling regimes at GABAergic synapses is unclear.
Direct measurements of parvalbumin concentration and paired recordings in rodent
hippocampus and cerebellum revealed that parvalbumin affects synaptic dynamics
only when expressed at high levels. Modeling suggests that, in high concentrations,
parvalbumin may exert BAPTA-like effects, modulating nanodomain coupling via competition
with local saturation of endogenous fixed buffers.
author:
- first_name: Emmanuel
full_name: Eggermann, Emmanuel
last_name: Eggermann
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Eggermann E, Jonas PM. How the “slow” Ca(2+) buffer parvalbumin affects transmitter
release in nanodomain coupling regimes at GABAergic synapses. Nature Neuroscience.
2011;15:20-22. doi:10.1038/nn.3002
apa: Eggermann, E., & Jonas, P. M. (2011). How the “slow” Ca(2+) buffer parvalbumin
affects transmitter release in nanodomain coupling regimes at GABAergic synapses.
Nature Neuroscience. Nature Publishing Group. https://doi.org/10.1038/nn.3002
chicago: Eggermann, Emmanuel, and Peter M Jonas. “How the ‘Slow’ Ca(2+) Buffer Parvalbumin
Affects Transmitter Release in Nanodomain Coupling Regimes at GABAergic Synapses.”
Nature Neuroscience. Nature Publishing Group, 2011. https://doi.org/10.1038/nn.3002.
ieee: E. Eggermann and P. M. Jonas, “How the ‘slow’ Ca(2+) buffer parvalbumin affects
transmitter release in nanodomain coupling regimes at GABAergic synapses,” Nature
Neuroscience, vol. 15. Nature Publishing Group, pp. 20–22, 2011.
ista: Eggermann E, Jonas PM. 2011. How the “slow” Ca(2+) buffer parvalbumin affects
transmitter release in nanodomain coupling regimes at GABAergic synapses. Nature
Neuroscience. 15, 20–22.
mla: Eggermann, Emmanuel, and Peter M. Jonas. “How the ‘Slow’ Ca(2+) Buffer Parvalbumin
Affects Transmitter Release in Nanodomain Coupling Regimes at GABAergic Synapses.”
Nature Neuroscience, vol. 15, Nature Publishing Group, 2011, pp. 20–22,
doi:10.1038/nn.3002.
short: E. Eggermann, P.M. Jonas, Nature Neuroscience 15 (2011) 20–22.
date_created: 2018-12-11T12:02:38Z
date_published: 2011-12-04T00:00:00Z
date_updated: 2021-01-12T07:42:37Z
day: '04'
department:
- _id: PeJo
doi: 10.1038/nn.3002
intvolume: ' 15'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3631701/
month: '12'
oa: 1
oa_version: Submitted Version
page: 20 - 22
publication: Nature Neuroscience
publication_status: published
publisher: Nature Publishing Group
publist_id: '3321'
quality_controlled: '1'
scopus_import: 1
status: public
title: How the “slow” Ca(2+) buffer parvalbumin affects transmitter release in nanodomain
coupling regimes at GABAergic synapses
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 15
year: '2011'
...
---
_id: '3369'
abstract:
- lang: eng
text: Rab3 interacting molecules (RIMs) are highly enriched in the active zones
of presynaptic terminals. It is generally thought that they operate as effectors
of the small G protein Rab3. Three recent papers, by Han et al. (this issue of
Neuron), Deng et al. (this issue of Neuron), and Kaeser et al. (a recent issue
of Cell), shed new light on the functional role of RIM in presynaptic terminals.
First, RIM tethers Ca2+ channels to active zones. Second, RIM contributes to priming
of synaptic vesicles by interacting with another presynaptic protein, Munc13.
author:
- first_name: Alejandro
full_name: Pernia-Andrade, Alejandro
id: 36963E98-F248-11E8-B48F-1D18A9856A87
last_name: Pernia-Andrade
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Pernia-Andrade A, Jonas PM. The multiple faces of RIM. Neuron. 2011;69(2):185-187.
doi:10.1016/j.neuron.2011.01.010
apa: Pernia-Andrade, A., & Jonas, P. M. (2011). The multiple faces of RIM. Neuron.
Elsevier. https://doi.org/10.1016/j.neuron.2011.01.010
chicago: Pernia-Andrade, Alejandro, and Peter M Jonas. “The Multiple Faces of RIM.”
Neuron. Elsevier, 2011. https://doi.org/10.1016/j.neuron.2011.01.010.
ieee: A. Pernia-Andrade and P. M. Jonas, “The multiple faces of RIM,” Neuron,
vol. 69, no. 2. Elsevier, pp. 185–187, 2011.
ista: Pernia-Andrade A, Jonas PM. 2011. The multiple faces of RIM. Neuron. 69(2),
185–187.
mla: Pernia-Andrade, Alejandro, and Peter M. Jonas. “The Multiple Faces of RIM.”
Neuron, vol. 69, no. 2, Elsevier, 2011, pp. 185–87, doi:10.1016/j.neuron.2011.01.010.
short: A. Pernia-Andrade, P.M. Jonas, Neuron 69 (2011) 185–187.
date_created: 2018-12-11T12:02:56Z
date_published: 2011-01-27T00:00:00Z
date_updated: 2021-01-12T07:43:00Z
day: '27'
department:
- _id: PeJo
doi: 10.1016/j.neuron.2011.01.010
intvolume: ' 69'
issue: '2'
language:
- iso: eng
month: '01'
oa_version: None
page: 185 - 187
publication: Neuron
publication_status: published
publisher: Elsevier
publist_id: '3243'
quality_controlled: '1'
scopus_import: 1
status: public
title: The multiple faces of RIM
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 69
year: '2011'
...
---
_id: '469'
abstract:
- lang: eng
text: 'Spontaneous release of glutamate is important for maintaining synaptic strength
and controlling spike timing in the brain. Mechanisms regulating spontaneous exocytosis
remain poorly understood. Extracellular calcium concentration ([Ca2+]o) regulates
Ca2+ entry through voltage-activated calcium channels (VACCs) and consequently
is a pivotal determinant of action potential-evoked vesicle fusion. Extracellular
Ca 2+ also enhances spontaneous release, but via unknown mechanisms. Here we report
that external Ca2+ triggers spontaneous glutamate release more weakly than evoked
release in mouse neocortical neurons. Blockade of VACCs has no effect on the spontaneous
release rate or its dependence on [Ca2+]o. Intracellular [Ca2+] slowly increases
in a minority of neurons following increases in [Ca2+]o. Furthermore, the enhancement
of spontaneous release by extracellular calcium is insensitive to chelation of
intracellular calcium by BAPTA. Activation of the calcium-sensing receptor (CaSR),
a G-protein-coupled receptor present in nerve terminals, by several specific agonists
increased spontaneous glutamate release. The frequency of spontaneous synaptic
transmission was decreased in CaSR mutant neurons. The concentration-effect relationship
for extracellular calcium regulation of spontaneous release was well described
by a combination of CaSR-dependent and CaSR-independent mechanisms. Overall these
results indicate that extracellular Ca2+ does not trigger spontaneous glutamate
release by simply increasing calcium influx but stimulates CaSR and thereby promotes
resting spontaneous glutamate release. '
author:
- first_name: Nicholas
full_name: Vyleta, Nicholas
id: 36C4978E-F248-11E8-B48F-1D18A9856A87
last_name: Vyleta
- first_name: Stephen
full_name: Smith, Stephen
last_name: Smith
citation:
ama: Vyleta N, Smith S. Spontaneous glutamate release is independent of calcium
influx and tonically activated by the calcium-sensing receptor. European Journal
of Neuroscience. 2011;31(12):4593-4606. doi:10.1523/JNEUROSCI.6398-10.2011
apa: Vyleta, N., & Smith, S. (2011). Spontaneous glutamate release is independent
of calcium influx and tonically activated by the calcium-sensing receptor. European
Journal of Neuroscience. Wiley-Blackwell. https://doi.org/10.1523/JNEUROSCI.6398-10.2011
chicago: Vyleta, Nicholas, and Stephen Smith. “Spontaneous Glutamate Release Is
Independent of Calcium Influx and Tonically Activated by the Calcium-Sensing Receptor.”
European Journal of Neuroscience. Wiley-Blackwell, 2011. https://doi.org/10.1523/JNEUROSCI.6398-10.2011.
ieee: N. Vyleta and S. Smith, “Spontaneous glutamate release is independent of calcium
influx and tonically activated by the calcium-sensing receptor,” European Journal
of Neuroscience, vol. 31, no. 12. Wiley-Blackwell, pp. 4593–4606, 2011.
ista: Vyleta N, Smith S. 2011. Spontaneous glutamate release is independent of calcium
influx and tonically activated by the calcium-sensing receptor. European Journal
of Neuroscience. 31(12), 4593–4606.
mla: Vyleta, Nicholas, and Stephen Smith. “Spontaneous Glutamate Release Is Independent
of Calcium Influx and Tonically Activated by the Calcium-Sensing Receptor.” European
Journal of Neuroscience, vol. 31, no. 12, Wiley-Blackwell, 2011, pp. 4593–606,
doi:10.1523/JNEUROSCI.6398-10.2011.
short: N. Vyleta, S. Smith, European Journal of Neuroscience 31 (2011) 4593–4606.
date_created: 2018-12-11T11:46:39Z
date_published: 2011-03-23T00:00:00Z
date_updated: 2021-01-12T08:00:49Z
day: '23'
department:
- _id: PeJo
doi: 10.1523/JNEUROSCI.6398-10.2011
intvolume: ' 31'
issue: '12'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3097128/
month: '03'
oa: 1
oa_version: Submitted Version
page: 4593 - 4606
publication: European Journal of Neuroscience
publication_status: published
publisher: Wiley-Blackwell
publist_id: '7353'
quality_controlled: '1'
scopus_import: 1
status: public
title: Spontaneous glutamate release is independent of calcium influx and tonically
activated by the calcium-sensing receptor
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 31
year: '2011'
...
---
_id: '490'
abstract:
- lang: eng
text: 'BioSig is an open source software library for biomedical signal processing.
The aim of the BioSig project is to foster research in biomedical signal processing
by providing free and open source software tools for many different application
areas. Some of the areas where BioSig can be employed are neuroinformatics, brain-computer
interfaces, neurophysiology, psychology, cardiovascular systems, and sleep research.
Moreover, the analysis of biosignals such as the electroencephalogram (EEG), electrocorticogram
(ECoG), electrocardiogram (ECG), electrooculogram (EOG), electromyogram (EMG),
or respiration signals is a very relevant element of the BioSig project. Specifically,
BioSig provides solutions for data acquisition, artifact processing, quality control,
feature extraction, classification, modeling, and data visualization, to name
a few. In this paper, we highlight several methods to help students and researchers
to work more efficiently with biomedical signals. '
article_number: '935364'
author:
- first_name: Alois
full_name: Schlögl, Alois
id: 45BF87EE-F248-11E8-B48F-1D18A9856A87
last_name: Schlögl
orcid: 0000-0002-5621-8100
- first_name: Carmen
full_name: Vidaurre, Carmen
last_name: Vidaurre
- first_name: Tilmann
full_name: Sander, Tilmann
last_name: Sander
citation:
ama: 'Schlögl A, Vidaurre C, Sander T. BioSig: The free and open source software
library for biomedical signal processing. Computational Intelligence and Neuroscience.
2011;2011. doi:10.1155/2011/935364'
apa: 'Schlögl, A., Vidaurre, C., & Sander, T. (2011). BioSig: The free and open
source software library for biomedical signal processing. Computational Intelligence
and Neuroscience. Hindawi Publishing Corporation. https://doi.org/10.1155/2011/935364'
chicago: 'Schlögl, Alois, Carmen Vidaurre, and Tilmann Sander. “BioSig: The Free
and Open Source Software Library for Biomedical Signal Processing.” Computational
Intelligence and Neuroscience. Hindawi Publishing Corporation, 2011. https://doi.org/10.1155/2011/935364.'
ieee: 'A. Schlögl, C. Vidaurre, and T. Sander, “BioSig: The free and open source
software library for biomedical signal processing,” Computational Intelligence
and Neuroscience, vol. 2011. Hindawi Publishing Corporation, 2011.'
ista: 'Schlögl A, Vidaurre C, Sander T. 2011. BioSig: The free and open source software
library for biomedical signal processing. Computational Intelligence and Neuroscience.
2011, 935364.'
mla: 'Schlögl, Alois, et al. “BioSig: The Free and Open Source Software Library
for Biomedical Signal Processing.” Computational Intelligence and Neuroscience,
vol. 2011, 935364, Hindawi Publishing Corporation, 2011, doi:10.1155/2011/935364.'
short: A. Schlögl, C. Vidaurre, T. Sander, Computational Intelligence and Neuroscience
2011 (2011).
date_created: 2018-12-11T11:46:45Z
date_published: 2011-01-01T00:00:00Z
date_updated: 2021-01-12T08:01:02Z
day: '01'
ddc:
- '005'
department:
- _id: ScienComp
- _id: PeJo
doi: 10.1155/2011/935364
file:
- access_level: open_access
checksum: 8263bbf255171f2054f43f3db5f53b6e
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:07:44Z
date_updated: 2020-07-14T12:46:35Z
file_id: '4642'
file_name: IST-2018-947-v1+1_2011_Schloegl_BioSig.pdf
file_size: 2863551
relation: main_file
file_date_updated: 2020-07-14T12:46:35Z
has_accepted_license: '1'
intvolume: ' 2011'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
publication: Computational Intelligence and Neuroscience
publication_status: published
publisher: Hindawi Publishing Corporation
publist_id: '7330'
pubrep_id: '947'
quality_controlled: '1'
scopus_import: 1
status: public
title: 'BioSig: The free and open source software library for biomedical signal processing'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 2011
year: '2011'
...
---
_id: '3718'
abstract:
- lang: eng
text: Long-term depression (LTD) is a form of synaptic plasticity that may contribute
to information storage in the central nervous system. Here we report that LTD
can be elicited in layer 5 pyramidal neurons of the rat prefrontal cortex by pairing
low frequency stimulation with a modest postsynaptic depolarization. The induction
of LTD required the activation of both metabotropic glutamate receptors of the
mGlu1 subtype and voltage-sensitive Ca(2+) channels (VSCCs) of the T/R, P/Q and
N types, leading to the stimulation of intracellular inositol trisphosphate (IP3)
receptors by IP3 and Ca(2+). The subsequent release of Ca(2+) from intracellular
stores activated the protein phosphatase cascade involving calcineurin and protein
phosphatase 1. The activation of purinergic P2Y(1) receptors blocked LTD. This
effect was prevented by P2Y(1) receptor antagonists and was absent in mice lacking
P2Y(1) but not P2Y(2) receptors. We also found that activation of P2Y(1) receptors
inhibits Ca(2+) transients via VSCCs in the apical dendrites and spines of pyramidal
neurons. In addition, we show that the release of ATP under hypoxia is able to
inhibit LTD by acting on postsynaptic P2Y(1) receptors. In conclusion, these data
suggest that the reduction of Ca(2+) influx via VSCCs caused by the activation
of P2Y(1) receptors by ATP is the possible mechanism for the inhibition of LTD
in prefrontal cortex.
acknowledgement: " The financial support of the Deutsche Forschungsgemeinschaft (IL
20/12-1, KI 677/2-4) is gratefully acknowledged.\r\nWe thank B. H. Koller (Department
of Genetics and Molecular Biology, University of North Carolina at Chapel Hill,
NC, USA) for the generous supply of P2Y1−/− and P2Y2−/− mice. We are grateful to
Dr. A. Schulz for reanalysing the genotype of the P2Y1−/− mice. The authors thank
P. Jonas and U. Heinemann for many helpful comments and A-K. Krause, L Feige and
M. Eberts for their excellent technical support."
author:
- first_name: José
full_name: Guzmán, José
id: 30CC5506-F248-11E8-B48F-1D18A9856A87
last_name: Guzmán
- first_name: Hartmut
full_name: Schmidt, Hartmut
last_name: Schmidt
- first_name: Heike
full_name: Franke, Heike
last_name: Franke
- first_name: Ute
full_name: Krügel, Ute
last_name: Krügel
- first_name: Jens
full_name: Eilers, Jens
last_name: Eilers
- first_name: Peter
full_name: Illes, Peter
last_name: Illes
- first_name: Zoltan
full_name: Gerevich, Zoltan
last_name: Gerevich
citation:
ama: Guzmán J, Schmidt H, Franke H, et al. P2Y1 receptors inhibit long-term depression
in the prefrontal cortex. Neuropharmacology. 2010;59(6):406-415. doi:10.1016/j.neuropharm.2010.05.013
apa: Guzmán, J., Schmidt, H., Franke, H., Krügel, U., Eilers, J., Illes, P., &
Gerevich, Z. (2010). P2Y1 receptors inhibit long-term depression in the prefrontal
cortex. Neuropharmacology. Elsevier. https://doi.org/10.1016/j.neuropharm.2010.05.013
chicago: Guzmán, José, Hartmut Schmidt, Heike Franke, Ute Krügel, Jens Eilers, Peter
Illes, and Zoltan Gerevich. “P2Y1 Receptors Inhibit Long-Term Depression in the
Prefrontal Cortex.” Neuropharmacology. Elsevier, 2010. https://doi.org/10.1016/j.neuropharm.2010.05.013.
ieee: J. Guzmán et al., “P2Y1 receptors inhibit long-term depression in the
prefrontal cortex.,” Neuropharmacology, vol. 59, no. 6. Elsevier, pp. 406–415,
2010.
ista: Guzmán J, Schmidt H, Franke H, Krügel U, Eilers J, Illes P, Gerevich Z. 2010.
P2Y1 receptors inhibit long-term depression in the prefrontal cortex. Neuropharmacology.
59(6), 406–415.
mla: Guzmán, José, et al. “P2Y1 Receptors Inhibit Long-Term Depression in the Prefrontal
Cortex.” Neuropharmacology, vol. 59, no. 6, Elsevier, 2010, pp. 406–15,
doi:10.1016/j.neuropharm.2010.05.013.
short: J. Guzmán, H. Schmidt, H. Franke, U. Krügel, J. Eilers, P. Illes, Z. Gerevich,
Neuropharmacology 59 (2010) 406–415.
date_created: 2018-12-11T12:04:47Z
date_published: 2010-11-01T00:00:00Z
date_updated: 2021-01-12T07:51:42Z
day: '01'
department:
- _id: PeJo
doi: 10.1016/j.neuropharm.2010.05.013
intvolume: ' 59'
issue: '6'
language:
- iso: eng
month: '11'
oa_version: None
page: 406 - 415
publication: Neuropharmacology
publication_status: published
publisher: Elsevier
publist_id: '2512'
quality_controlled: '1'
scopus_import: 1
status: public
title: P2Y1 receptors inhibit long-term depression in the prefrontal cortex.
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 59
year: '2010'
...
---
_id: '3832'
abstract:
- lang: eng
text: A recent paper by von Engelhardt et al. identifies a novel auxiliary subunit
of native AMPARs, termedCKAMP44. Unlike other auxiliary subunits, CKAMP44 accelerates
desensitization and prolongs recovery from desensitization. CKAMP44 is highly
expressed in hippocampal dentate gyrus granule cells and decreases the paired-pulse
ratio at perforant path input synapses. Thus, both principal and auxiliary AMPAR
subunits control the time course of signaling at glutamatergic synapses.
article_processing_charge: No
author:
- first_name: José
full_name: Guzmán, José
id: 30CC5506-F248-11E8-B48F-1D18A9856A87
last_name: Guzmán
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: 'Guzmán J, Jonas PM. Beyond TARPs: The growing list of auxiliary AMPAR subunits.
Neuron. 2010;66(1):8-10. doi:10.1016/j.neuron.2010.04.003'
apa: 'Guzmán, J., & Jonas, P. M. (2010). Beyond TARPs: The growing list of auxiliary
AMPAR subunits. Neuron. Elsevier. https://doi.org/10.1016/j.neuron.2010.04.003'
chicago: 'Guzmán, José, and Peter M Jonas. “Beyond TARPs: The Growing List of Auxiliary
AMPAR Subunits.” Neuron. Elsevier, 2010. https://doi.org/10.1016/j.neuron.2010.04.003.'
ieee: 'J. Guzmán and P. M. Jonas, “Beyond TARPs: The growing list of auxiliary AMPAR
subunits,” Neuron, vol. 66, no. 1. Elsevier, pp. 8–10, 2010.'
ista: 'Guzmán J, Jonas PM. 2010. Beyond TARPs: The growing list of auxiliary AMPAR
subunits. Neuron. 66(1), 8–10.'
mla: 'Guzmán, José, and Peter M. Jonas. “Beyond TARPs: The Growing List of Auxiliary
AMPAR Subunits.” Neuron, vol. 66, no. 1, Elsevier, 2010, pp. 8–10, doi:10.1016/j.neuron.2010.04.003.'
short: J. Guzmán, P.M. Jonas, Neuron 66 (2010) 8–10.
date_created: 2018-12-11T12:05:25Z
date_published: 2010-04-15T00:00:00Z
date_updated: 2021-01-12T07:52:31Z
day: '15'
department:
- _id: PeJo
doi: 10.1016/j.neuron.2010.04.003
external_id:
pmid:
- '20399724'
intvolume: ' 66'
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pubmed/20399724
month: '04'
oa: 1
oa_version: Published Version
page: 8 - 10
pmid: 1
publication: Neuron
publication_status: published
publisher: Elsevier
publist_id: '2377'
quality_controlled: '1'
scopus_import: 1
status: public
title: 'Beyond TARPs: The growing list of auxiliary AMPAR subunits'
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 66
year: '2010'
...
---
_id: '3833'
article_processing_charge: No
author:
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: Stefan
full_name: Hefft, Stefan
last_name: Hefft
citation:
ama: 'Jonas PM, Hefft S. GABA release at terminals of CCK-interneurons: synchrony,
asynchrony and modulation by cannabinoid receptors (commentary on Ali &
Todorova). The European Journal of Neuroscience. 2010;31(7):1194-1195.
doi:10.1111/j.1460-9568.2010.07189.x'
apa: 'Jonas, P. M., & Hefft, S. (2010). GABA release at terminals of CCK-interneurons:
synchrony, asynchrony and modulation by cannabinoid receptors (commentary on Ali
& Todorova). The European Journal of Neuroscience. Wiley-Blackwell.
https://doi.org/10.1111/j.1460-9568.2010.07189.x'
chicago: 'Jonas, Peter M, and Stefan Hefft. “GABA Release at Terminals of CCK-Interneurons:
Synchrony, Asynchrony and Modulation by Cannabinoid Receptors (Commentary on Ali
& Todorova).” The European Journal of Neuroscience. Wiley-Blackwell,
2010. https://doi.org/10.1111/j.1460-9568.2010.07189.x.'
ieee: 'P. M. Jonas and S. Hefft, “GABA release at terminals of CCK-interneurons:
synchrony, asynchrony and modulation by cannabinoid receptors (commentary on Ali
& Todorova),” The European Journal of Neuroscience, vol. 31, no.
7. Wiley-Blackwell, pp. 1194–1195, 2010.'
ista: 'Jonas PM, Hefft S. 2010. GABA release at terminals of CCK-interneurons: synchrony,
asynchrony and modulation by cannabinoid receptors (commentary on Ali &
Todorova). The European Journal of Neuroscience. 31(7), 1194–1195.'
mla: 'Jonas, Peter M., and Stefan Hefft. “GABA Release at Terminals of CCK-Interneurons:
Synchrony, Asynchrony and Modulation by Cannabinoid Receptors (Commentary on Ali
& Todorova).” The European Journal of Neuroscience, vol. 31, no.
7, Wiley-Blackwell, 2010, pp. 1194–95, doi:10.1111/j.1460-9568.2010.07189.x.'
short: P.M. Jonas, S. Hefft, The European Journal of Neuroscience 31 (2010) 1194–1195.
date_created: 2018-12-11T12:05:25Z
date_published: 2010-03-19T00:00:00Z
date_updated: 2021-01-12T07:52:31Z
day: '19'
department:
- _id: PeJo
doi: 10.1111/j.1460-9568.2010.07189.x
intvolume: ' 31'
issue: '7'
language:
- iso: eng
month: '03'
oa_version: None
page: 1194 - 1195
publication: The European Journal of Neuroscience
publication_status: published
publisher: Wiley-Blackwell
publist_id: '2378'
quality_controlled: '1'
scopus_import: 1
status: public
title: 'GABA release at terminals of CCK-interneurons: synchrony, asynchrony and modulation
by cannabinoid receptors (commentary on Ali & Todorova)'
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 31
year: '2010'
...