---
_id: '12716'
abstract:
- lang: eng
text: "The process of detecting and evaluating sensory information to guide behaviour
is termed perceptual decision-making (PDM), and is critical for the ability of
an organism to interact with its external world. Individuals with autism, a neurodevelopmental
condition primarily characterised by social and communication difficulties, frequently
exhibit altered sensory processing and PDM difficulties are widely reported. Recent
technological advancements have pushed forward our understanding of the genetic
changes accompanying this condition, however our understanding of how these mutations
affect the function of specific neuronal circuits and bring about the corresponding
behavioural changes remains limited. Here, we use an innate PDM task, the looming
avoidance response (LAR) paradigm, to identify a convergent behavioural abnormality
across three molecularly distinct genetic mouse models of autism (Cul3, Setd5
and Ptchd1). Although mutant mice can rapidly detect threatening visual stimuli,
their responses are consistently delayed, requiring longer to initiate an appropriate
response than their wild-type siblings. Mutant animals show abnormal adaptation
in both their stimulus- evoked escape responses and exploratory dynamics following
repeated stimulus presentations. Similarly delayed behavioural responses are observed
in wild-type animals when faced with more ambiguous threats, suggesting the mutant
phenotype could arise from a dysfunction in the flexible control of this PDM process.\r\nOur
knowledge of the core neuronal circuitry mediating the LAR facilitated a detailed
dissection of the neuronal mechanisms underlying the behavioural impairment. In
vivo extracellular recording revealed that visual responses were unaffected within
a key brain region for the rapid processing of visual threats, the superior colliculus
(SC), indicating that the behavioural delay was unlikely to originate from sensory
impairments. Delayed behavioural responses were recapitulated in the Setd5 model
following optogenetic stimulation of the excitatory output neurons of the SC,
which are known to mediate escape initiation through the activation of cells in
the underlying dorsal periaqueductal grey (dPAG). In vitro patch-clamp recordings
of dPAG cells uncovered a stark hypoexcitability phenotype in two out of the three
genetic models investigated (Setd5 and Ptchd1), that in Setd5, is mediated by
the misregulation of voltage-gated potassium channels. Overall, our results show
that the ability to use visual information to drive efficient escape responses
is impaired in three diverse genetic mouse models of autism and that, in one of
the models studied, this behavioural delay likely originates from differences
in the intrinsic excitability of a key subcortical node, the dPAG. Furthermore,
this work showcases the use of an innate behavioural paradigm to mechanistically
dissect PDM processes in autism."
acknowledged_ssus:
- _id: PreCl
- _id: Bio
- _id: LifeSc
- _id: M-Shop
- _id: CampIT
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Laura
full_name: Burnett, Laura
id: 3B717F68-F248-11E8-B48F-1D18A9856A87
last_name: Burnett
orcid: 0000-0002-8937-410X
citation:
ama: Burnett L. To flee, or not to flee? Using innate defensive behaviours to investigate
rapid perceptual decision-making through subcortical circuits in mouse models
of autism. 2023. doi:10.15479/at:ista:12716
apa: Burnett, L. (2023). To flee, or not to flee? Using innate defensive behaviours
to investigate rapid perceptual decision-making through subcortical circuits in
mouse models of autism. Institute of Science and Technology Austria. https://doi.org/10.15479/at:ista:12716
chicago: Burnett, Laura. “To Flee, or Not to Flee? Using Innate Defensive Behaviours
to Investigate Rapid Perceptual Decision-Making through Subcortical Circuits in
Mouse Models of Autism.” Institute of Science and Technology Austria, 2023. https://doi.org/10.15479/at:ista:12716.
ieee: L. Burnett, “To flee, or not to flee? Using innate defensive behaviours to
investigate rapid perceptual decision-making through subcortical circuits in mouse
models of autism,” Institute of Science and Technology Austria, 2023.
ista: Burnett L. 2023. To flee, or not to flee? Using innate defensive behaviours
to investigate rapid perceptual decision-making through subcortical circuits in
mouse models of autism. Institute of Science and Technology Austria.
mla: Burnett, Laura. To Flee, or Not to Flee? Using Innate Defensive Behaviours
to Investigate Rapid Perceptual Decision-Making through Subcortical Circuits in
Mouse Models of Autism. Institute of Science and Technology Austria, 2023,
doi:10.15479/at:ista:12716.
short: L. Burnett, To Flee, or Not to Flee? Using Innate Defensive Behaviours to
Investigate Rapid Perceptual Decision-Making through Subcortical Circuits in Mouse
Models of Autism, Institute of Science and Technology Austria, 2023.
date_created: 2023-03-08T15:19:45Z
date_published: 2023-03-10T00:00:00Z
date_updated: 2023-04-05T10:59:04Z
day: '10'
ddc:
- '599'
- '573'
degree_awarded: PhD
department:
- _id: GradSch
- _id: MaJö
doi: 10.15479/at:ista:12716
ec_funded: 1
file:
- access_level: closed
checksum: 6c6d9cc2c4cdacb74e6b1047a34d7332
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: lburnett
date_created: 2023-03-08T15:08:46Z
date_updated: 2023-03-08T15:08:46Z
file_id: '12717'
file_name: Burnett_Thesis_2023.docx
file_size: 23029260
relation: source_file
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checksum: cebc77705288bf4382db9b3541483cd0
content_type: application/pdf
creator: lburnett
date_created: 2023-03-08T15:08:46Z
date_updated: 2023-03-08T15:08:46Z
file_id: '12718'
file_name: Burnett_Thesis_2023_pdfA.pdf
file_size: 11959869
relation: main_file
success: 1
file_date_updated: 2023-03-08T15:08:46Z
has_accepted_license: '1'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
page: '178'
project:
- _id: 2634E9D2-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '756502'
name: Circuits of Visual Attention
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
status: public
supervisor:
- first_name: Maximilian A
full_name: Jösch, Maximilian A
id: 2BD278E6-F248-11E8-B48F-1D18A9856A87
last_name: Jösch
orcid: 0000-0002-3937-1330
title: To flee, or not to flee? Using innate defensive behaviours to investigate rapid
perceptual decision-making through subcortical circuits in mouse models of autism
type: dissertation
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
year: '2023'
...
---
_id: '12826'
abstract:
- lang: eng
text: "During navigation, animals can infer the structure of the environment by
computing the optic flow cues elicited by their own movements, and subsequently
use this information to instruct proper locomotor actions. These computations
require a panoramic assessment of the visual environment in order to disambiguate
similar sensory experiences that may require distinct behavioral responses. The
estimation of the global motion patterns is therefore essential for successful
navigation. Yet, our understanding of the algorithms and implementations that
enable coherent panoramic visual perception remains scarce. Here I pursue this
problem by dissecting the functional aspects of interneuronal communication in
the lobula plate tangential cell network in Drosophila melanogaster. The results
presented in the thesis demonstrate that the basis for effective interpretation
of the optic flow in this circuit are stereotyped synaptic connections that mediate
the formation of distinct subnetworks, each extracting a particular pattern of
global motion. \r\nFirstly, I show that gap junctions are essential for a correct
interpretation of binocular motion cues by horizontal motion-sensitive cells.
HS cells form electrical synapses with contralateral H2 neurons that are involved
in detecting yaw rotation and translation. I developed an FlpStop-mediated mutant
of a gap junction protein ShakB that disrupts these electrical synapses. While
the loss of electrical synapses does not affect the tuning of the direction selectivity
in HS neurons, it severely alters their sensitivity to horizontal motion in the
contralateral side. These physiological changes result in an inappropriate integration
of binocular motion cues in walking animals. While wild-type flies form a binocular
perception of visual motion by non-linear integration of monocular optic flow
cues, the mutant flies sum the monocular inputs linearly. These results indicate
that rather than averaging signals in neighboring neurons, gap-junctions operate
in conjunction with chemical synapses to mediate complex non-linear optic flow
computations.\r\nSecondly, I show that stochastic manipulation of neuronal activity
in the lobula plate tangential cell network is a powerful approach to study the
neuronal implementation of optic flow-based navigation in flies. Tangential neurons
form multiple subnetworks, each mediating course-stabilizing response to a particular
global pattern of visual motion. Application of genetic mosaic techniques can
provide sparse optogenetic activation of HS cells in numerous combinations. These
distinct combinations of activated neurons drive an array of distinct behavioral
responses, providing important insights into how visuomotor transformation is
performed in the lobula plate tangential cell network. This approach can be complemented
by stochastic silencing of tangential neurons, enabling direct assessment of the
functional role of individual tangential neurons in the processing of specific
visual motion patterns.\r\n\tTaken together, the findings presented in this thesis
suggest that establishing specific activity patterns of tangential cells via stereotyped
synaptic connectivity is a key to efficient optic flow-based navigation in Drosophila
melanogaster."
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Victoria
full_name: Pokusaeva, Victoria
id: 3184041C-F248-11E8-B48F-1D18A9856A87
last_name: Pokusaeva
orcid: 0000-0001-7660-444X
citation:
ama: Pokusaeva V. Neural control of optic flow-based navigation in Drosophila melanogaster.
2023. doi:10.15479/at:ista:12826
apa: Pokusaeva, V. (2023). Neural control of optic flow-based navigation in Drosophila
melanogaster. Institute of Science and Technology Austria. https://doi.org/10.15479/at:ista:12826
chicago: Pokusaeva, Victoria. “Neural Control of Optic Flow-Based Navigation in
Drosophila Melanogaster.” Institute of Science and Technology Austria, 2023. https://doi.org/10.15479/at:ista:12826.
ieee: V. Pokusaeva, “Neural control of optic flow-based navigation in Drosophila
melanogaster,” Institute of Science and Technology Austria, 2023.
ista: Pokusaeva V. 2023. Neural control of optic flow-based navigation in Drosophila
melanogaster. Institute of Science and Technology Austria.
mla: Pokusaeva, Victoria. Neural Control of Optic Flow-Based Navigation in Drosophila
Melanogaster. Institute of Science and Technology Austria, 2023, doi:10.15479/at:ista:12826.
short: V. Pokusaeva, Neural Control of Optic Flow-Based Navigation in Drosophila
Melanogaster, Institute of Science and Technology Austria, 2023.
date_created: 2023-04-14T14:56:04Z
date_published: 2023-04-18T00:00:00Z
date_updated: 2023-06-23T09:47:36Z
day: '18'
ddc:
- '570'
- '571'
degree_awarded: PhD
department:
- _id: MaJö
- _id: GradSch
doi: 10.15479/at:ista:12826
ec_funded: 1
file:
- access_level: closed
checksum: 5f589a9af025f7eeebfd0c186209913e
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: vpokusae
date_created: 2023-04-20T09:14:38Z
date_updated: 2023-04-20T09:26:51Z
file_id: '12857'
file_name: Thesis_Pokusaeva.docx
file_size: 14507243
relation: source_file
- access_level: open_access
checksum: bbeed76db45a996b4c91a9abe12ce0ec
content_type: application/pdf
creator: vpokusae
date_created: 2023-04-20T09:14:44Z
date_updated: 2023-04-20T09:14:44Z
file_id: '12858'
file_name: Thesis_Pokusaeva.pdf
file_size: 10090711
relation: main_file
success: 1
file_date_updated: 2023-04-20T09:26:51Z
has_accepted_license: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '04'
oa: 1
oa_version: Published Version
page: '106'
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
publication_identifier:
issn:
- 2663 - 337X
publication_status: published
publisher: Institute of Science and Technology Austria
status: public
supervisor:
- first_name: Maximilian A
full_name: Jösch, Maximilian A
id: 2BD278E6-F248-11E8-B48F-1D18A9856A87
last_name: Jösch
orcid: 0000-0002-3937-1330
title: Neural control of optic flow-based navigation in Drosophila melanogaster
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: dissertation
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
year: '2023'
...
---
_id: '13230'
abstract:
- lang: eng
text: 'To interpret the sensory environment, the brain combines ambiguous sensory
measurements with knowledge that reflects context-specific prior experience. But
environmental contexts can change abruptly and unpredictably, resulting in uncertainty
about the current context. Here we address two questions: how should context-specific
prior knowledge optimally guide the interpretation of sensory stimuli in changing
environments, and do human decision-making strategies resemble this optimum? We
probe these questions with a task in which subjects report the orientation of
ambiguous visual stimuli that were drawn from three dynamically switching distributions,
representing different environmental contexts. We derive predictions for an ideal
Bayesian observer that leverages knowledge about the statistical structure of
the task to maximize decision accuracy, including knowledge about the dynamics
of the environment. We show that its decisions are biased by the dynamically changing
task context. The magnitude of this decision bias depends on the observer’s continually
evolving belief about the current context. The model therefore not only predicts
that decision bias will grow as the context is indicated more reliably, but also
as the stability of the environment increases, and as the number of trials since
the last context switch grows. Analysis of human choice data validates all three
predictions, suggesting that the brain leverages knowledge of the statistical
structure of environmental change when interpreting ambiguous sensory signals.'
acknowledgement: The authors thank Corey Ziemba and Zoe Boundy-Singer for valuable
discussion and feedback.
article_number: e1011104
article_processing_charge: No
article_type: original
author:
- first_name: Julie A.
full_name: Charlton, Julie A.
last_name: Charlton
- first_name: Wiktor F
full_name: Mlynarski, Wiktor F
id: 358A453A-F248-11E8-B48F-1D18A9856A87
last_name: Mlynarski
- first_name: Yoon H.
full_name: Bai, Yoon H.
last_name: Bai
- first_name: Ann M.
full_name: Hermundstad, Ann M.
last_name: Hermundstad
- first_name: Robbe L.T.
full_name: Goris, Robbe L.T.
last_name: Goris
citation:
ama: Charlton JA, Mlynarski WF, Bai YH, Hermundstad AM, Goris RLT. Environmental
dynamics shape perceptual decision bias. PLoS Computational Biology. 2023;19(6).
doi:10.1371/journal.pcbi.1011104
apa: Charlton, J. A., Mlynarski, W. F., Bai, Y. H., Hermundstad, A. M., & Goris,
R. L. T. (2023). Environmental dynamics shape perceptual decision bias. PLoS
Computational Biology. Public Library of Science. https://doi.org/10.1371/journal.pcbi.1011104
chicago: Charlton, Julie A., Wiktor F Mlynarski, Yoon H. Bai, Ann M. Hermundstad,
and Robbe L.T. Goris. “Environmental Dynamics Shape Perceptual Decision Bias.”
PLoS Computational Biology. Public Library of Science, 2023. https://doi.org/10.1371/journal.pcbi.1011104.
ieee: J. A. Charlton, W. F. Mlynarski, Y. H. Bai, A. M. Hermundstad, and R. L. T.
Goris, “Environmental dynamics shape perceptual decision bias,” PLoS Computational
Biology, vol. 19, no. 6. Public Library of Science, 2023.
ista: Charlton JA, Mlynarski WF, Bai YH, Hermundstad AM, Goris RLT. 2023. Environmental
dynamics shape perceptual decision bias. PLoS Computational Biology. 19(6), e1011104.
mla: Charlton, Julie A., et al. “Environmental Dynamics Shape Perceptual Decision
Bias.” PLoS Computational Biology, vol. 19, no. 6, e1011104, Public Library
of Science, 2023, doi:10.1371/journal.pcbi.1011104.
short: J.A. Charlton, W.F. Mlynarski, Y.H. Bai, A.M. Hermundstad, R.L.T. Goris,
PLoS Computational Biology 19 (2023).
date_created: 2023-07-16T22:01:09Z
date_published: 2023-06-08T00:00:00Z
date_updated: 2023-08-02T06:33:50Z
day: '08'
ddc:
- '570'
department:
- _id: MaJö
doi: 10.1371/journal.pcbi.1011104
external_id:
isi:
- '001003410200003'
pmid:
- '37289753'
file:
- access_level: open_access
checksum: 800761fa2c647fabd6ad034589bc526e
content_type: application/pdf
creator: dernst
date_created: 2023-07-18T08:07:59Z
date_updated: 2023-07-18T08:07:59Z
file_id: '13247'
file_name: 2023_PloSCompBio_Charlton.pdf
file_size: 2281868
relation: main_file
success: 1
file_date_updated: 2023-07-18T08:07:59Z
has_accepted_license: '1'
intvolume: ' 19'
isi: 1
issue: '6'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
pmid: 1
publication: PLoS Computational Biology
publication_identifier:
eissn:
- 1553-7358
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
scopus_import: '1'
status: public
title: Environmental dynamics shape perceptual decision bias
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 19
year: '2023'
...
---
_id: '12349'
abstract:
- lang: eng
text: Statistics of natural scenes are not uniform - their structure varies dramatically
from ground to sky. It remains unknown whether these non-uniformities are reflected
in the large-scale organization of the early visual system and what benefits such
adaptations would confer. Here, by relying on the efficient coding hypothesis,
we predict that changes in the structure of receptive fields across visual space
increase the efficiency of sensory coding. We show experimentally that, in agreement
with our predictions, receptive fields of retinal ganglion cells change their
shape along the dorsoventral retinal axis, with a marked surround asymmetry at
the visual horizon. Our work demonstrates that, according to principles of efficient
coding, the panoramic structure of natural scenes is exploited by the retina across
space and cell-types.
acknowledged_ssus:
- _id: ScienComp
- _id: PreCl
- _id: LifeSc
- _id: Bio
acknowledgement: We thank Hiroki Asari for sharing the dataset of naturalistic images,
Anton Sumser for sharing visual stimulus code, Yoav Ben Simon for initial explorative
work with the generation of AAVs, and Tomas Vega-Zuñiga for help with immunostainings.
We also thank Gasper Tkacik and members of the Neuroethology group for their comments
on the manuscript. This research was supported by the Scientific Service Units of
IST Austria through resources provided by Scientific Computing, the Preclinical
Facility, the Lab Support Facility, and the Imaging and Optics Facility. This work
was supported by European Union Horizon 2020 Marie Skłodowska-Curie grant 665385
(DG), Austrian Science Fund (FWF) stand-alone grant P 34015 (WM), Human Frontiers
Science Program LT000256/2018-L (AS), EMBO ALTF 1098-2017 (AS) and the European
Research Council Starting Grant 756502 (MJ).
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Divyansh
full_name: Gupta, Divyansh
id: 2A485EBE-F248-11E8-B48F-1D18A9856A87
last_name: Gupta
orcid: 0000-0001-7400-6665
- first_name: Wiktor F
full_name: Mlynarski, Wiktor F
id: 358A453A-F248-11E8-B48F-1D18A9856A87
last_name: Mlynarski
- first_name: Anton L
full_name: Sumser, Anton L
id: 3320A096-F248-11E8-B48F-1D18A9856A87
last_name: Sumser
orcid: 0000-0002-4792-1881
- first_name: Olga
full_name: Symonova, Olga
id: 3C0C7BC6-F248-11E8-B48F-1D18A9856A87
last_name: Symonova
orcid: 0000-0003-2012-9947
- first_name: Jan
full_name: Svaton, Jan
id: f7f724c3-9d6f-11ed-9f44-e5c5f3a5bee2
last_name: Svaton
orcid: 0000-0002-6198-2939
- first_name: Maximilian A
full_name: Jösch, Maximilian A
id: 2BD278E6-F248-11E8-B48F-1D18A9856A87
last_name: Jösch
orcid: 0000-0002-3937-1330
citation:
ama: Gupta D, Mlynarski WF, Sumser AL, Symonova O, Svaton J, Jösch MA. Panoramic
visual statistics shape retina-wide organization of receptive fields. Nature
Neuroscience. 2023;26:606-614. doi:10.1038/s41593-023-01280-0
apa: Gupta, D., Mlynarski, W. F., Sumser, A. L., Symonova, O., Svaton, J., &
Jösch, M. A. (2023). Panoramic visual statistics shape retina-wide organization
of receptive fields. Nature Neuroscience. Springer Nature. https://doi.org/10.1038/s41593-023-01280-0
chicago: Gupta, Divyansh, Wiktor F Mlynarski, Anton L Sumser, Olga Symonova, Jan
Svaton, and Maximilian A Jösch. “Panoramic Visual Statistics Shape Retina-Wide
Organization of Receptive Fields.” Nature Neuroscience. Springer Nature,
2023. https://doi.org/10.1038/s41593-023-01280-0.
ieee: D. Gupta, W. F. Mlynarski, A. L. Sumser, O. Symonova, J. Svaton, and M. A.
Jösch, “Panoramic visual statistics shape retina-wide organization of receptive
fields,” Nature Neuroscience, vol. 26. Springer Nature, pp. 606–614, 2023.
ista: Gupta D, Mlynarski WF, Sumser AL, Symonova O, Svaton J, Jösch MA. 2023. Panoramic
visual statistics shape retina-wide organization of receptive fields. Nature Neuroscience.
26, 606–614.
mla: Gupta, Divyansh, et al. “Panoramic Visual Statistics Shape Retina-Wide Organization
of Receptive Fields.” Nature Neuroscience, vol. 26, Springer Nature, 2023,
pp. 606–14, doi:10.1038/s41593-023-01280-0.
short: D. Gupta, W.F. Mlynarski, A.L. Sumser, O. Symonova, J. Svaton, M.A. Jösch,
Nature Neuroscience 26 (2023) 606–614.
date_created: 2023-01-23T14:14:19Z
date_published: 2023-04-01T00:00:00Z
date_updated: 2023-10-04T11:41:05Z
day: '01'
ddc:
- '570'
department:
- _id: GradSch
- _id: MaJö
doi: 10.1038/s41593-023-01280-0
ec_funded: 1
external_id:
isi:
- '000955258300002'
pmid:
- '36959418'
file:
- access_level: open_access
checksum: a33d91e398e548f34003170e10988368
content_type: application/pdf
creator: dernst
date_created: 2023-10-04T11:40:51Z
date_updated: 2023-10-04T11:40:51Z
file_id: '14395'
file_name: 2023_NatureNeuroscience_Gupta.pdf
file_size: 6144866
relation: main_file
success: 1
file_date_updated: 2023-10-04T11:40:51Z
has_accepted_license: '1'
intvolume: ' 26'
isi: 1
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
page: 606-614
pmid: 1
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
- _id: 626c45b5-2b32-11ec-9570-e509828c1ba6
grant_number: P34015
name: Efficient coding with biophysical realism
- _id: 2634E9D2-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '756502'
name: Circuits of Visual Attention
- _id: 266D407A-B435-11E9-9278-68D0E5697425
grant_number: LT000256
name: Neuronal networks of salience and spatial detection in the murine superior
colliculus
- _id: 264FEA02-B435-11E9-9278-68D0E5697425
grant_number: ALTF 1098-2017
name: Connecting sensory with motor processing in the superior colliculus
publication: Nature Neuroscience
publication_identifier:
eissn:
- 1546-1726
issn:
- 1097-6256
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
record:
- id: '12370'
relation: research_data
status: public
scopus_import: '1'
status: public
title: Panoramic visual statistics shape retina-wide organization of receptive fields
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 26
year: '2023'
...
---
_id: '12531'
abstract:
- lang: eng
text: "All visual experiences of the vertebrates begin with light being converted
into electrical signals\r\nby the eye retina. Retinal ganglion cells (RGCs) are
the neurons of the innermost layer of the\r\nmammal retina, and they transmit
visual information to the rest of the brain.\r\nIt has been shown that RGCs vary
in their morphology and genetic profiles, moreover they can\r\nbe unambiguously
grouped into subtypes that share the same morphological and/or molecular\r\nproperties.
However, in terms of RGCs function, it remains unclear how many distinct types\r\nthere
are and what response properties their typology relies on. Even given the recent
studies\r\nthat successfully classified RGCs in a patch of the retina [1] and
in scotopic conditions [2], the\r\nquestion remains whether the found subtypes
persist across the entire retina.\r\nIn this work, using a novel imaging method,
we show that, when sampled from a large portion\r\nof the retina, RGCs can not
be clearly divided into functional subtypes. We found that in\r\nphotopic conditions,
which implies more prominent natural scene statistic differences across\r\nthe
visual field, response properties can be exhibited by cells differently depending
on their\r\nlocation in the retina, which leads to formation of a gradient of
features rather than distinct\r\nclasses.\r\nThis finding suggests that RGCs follow
a global organization across the visual field of the\r\nanimal, adapting each
RGC subtype to the requirements imposed by the natural scene statistics."
alternative_title:
- ISTA Master's Thesis
article_processing_charge: No
author:
- first_name: Kseniia
full_name: Kirillova, Kseniia
id: 8e3f931e-dc85-11ea-9058-e7b957bf23f0
last_name: Kirillova
citation:
ama: Kirillova K. Panoramic functional gradients across the mouse retina. 2023.
doi:10.15479/at:ista:12531
apa: Kirillova, K. (2023). Panoramic functional gradients across the mouse retina.
Institute of Science and Technology Austria. https://doi.org/10.15479/at:ista:12531
chicago: Kirillova, Kseniia. “Panoramic Functional Gradients across the Mouse Retina.”
Institute of Science and Technology Austria, 2023. https://doi.org/10.15479/at:ista:12531.
ieee: K. Kirillova, “Panoramic functional gradients across the mouse retina,” Institute
of Science and Technology Austria, 2023.
ista: Kirillova K. 2023. Panoramic functional gradients across the mouse retina.
Institute of Science and Technology Austria.
mla: Kirillova, Kseniia. Panoramic Functional Gradients across the Mouse Retina.
Institute of Science and Technology Austria, 2023, doi:10.15479/at:ista:12531.
short: K. Kirillova, Panoramic Functional Gradients across the Mouse Retina, Institute
of Science and Technology Austria, 2023.
date_created: 2023-02-09T07:45:05Z
date_published: 2023-02-08T00:00:00Z
date_updated: 2024-02-09T23:30:04Z
day: '08'
ddc:
- '570'
degree_awarded: MS
department:
- _id: GradSch
- _id: MaJö
doi: 10.15479/at:ista:12531
file:
- access_level: open_access
checksum: 57d8da3a6c749eb1556b7435fe266a5f
content_type: application/pdf
creator: cchlebak
date_created: 2023-02-09T08:03:32Z
date_updated: 2024-02-09T23:30:03Z
embargo: 2024-02-08
file_id: '12532'
file_name: Thesis_Kseniia___ISTA__istaustriathesis_PDF-A.pdf
file_size: 8369317
relation: main_file
- access_level: closed
checksum: 87fb44318e4f9eb9da2ad9ad6ca8e76f
content_type: application/x-zip-compressed
creator: cchlebak
date_created: 2023-02-10T09:32:06Z
date_updated: 2024-02-09T23:30:03Z
embargo_to: open_access
file_id: '12535'
file_name: Thesis Kseniia - ISTA [istaustriathesis]-FINAL.zip
file_size: 11204408
relation: source_file
file_date_updated: 2024-02-09T23:30:03Z
has_accepted_license: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-sa/4.0/
month: '02'
oa: 1
oa_version: Published Version
page: '46'
publication_identifier:
issn:
- 2791-4585
publication_status: published
publisher: Institute of Science and Technology Austria
status: public
supervisor:
- first_name: Maximilian A
full_name: Jösch, Maximilian A
id: 2BD278E6-F248-11E8-B48F-1D18A9856A87
last_name: Jösch
orcid: 0000-0002-3937-1330
title: Panoramic functional gradients across the mouse retina
tmp:
image: /images/cc_by_nc_sa.png
legal_code_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC
BY-NC-SA 4.0)
short: CC BY-NC-SA (4.0)
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2023'
...
---
_id: '12226'
abstract:
- lang: eng
text: "Background: Biases of DNA repair can shape the nucleotide landscape of genomes
at evolutionary timescales. The molecular mechanisms of those biases are still
poorly understood because it is difficult to isolate the contributions of DNA
repair from those of DNA damage.\r\n\r\nResults: Here, we develop a genome-wide
assay whereby the same DNA lesion is repaired in different genomic contexts. We
insert thousands of barcoded transposons carrying a reporter of DNA mismatch repair
in the genome of mouse embryonic stem cells. Upon inducing a double-strand break
between tandem repeats, a mismatch is generated if the break is repaired through
single-strand annealing. The resolution of the mismatch showed a 60–80% bias in
favor of the strand with the longest 3′ flap. The location of the lesion in the
genome and the type of mismatch had little influence on the bias. Instead, we
observe a complete reversal of the bias when the longest 3′ flap is moved to the
opposite strand by changing the position of the double-strand break in the reporter.\r\n\r\nConclusions:
These results suggest that the processing of the double-strand break has a major
influence on the repair of mismatches during single-strand annealing."
acknowledgement: We acknowledge the financial support of the Natural Sciences and
Engineering Research Council of Canada (NSERC RGPIN-2020-06377), the Spanish Ministry
of Economy, Industry and Competitiveness (“Centro de Excelencia Severo Ochoa 2013-2017”,
Plan Estatal PGC2018-099807-B-I00), of the CERCA Programme/Generalitat de Catalunya,
and of the European Research Council (Synergy Grant 609989). VOP was supported by
the European Union’s Horizon 2020 research and innovation program under the Marie
Skłodowska-Curie programme (665385). We also acknowledge the support of the Spanish
Ministry of Economy and Competitiveness (MEIC) to the EMBL partnership.
article_number: '93'
article_processing_charge: No
article_type: original
author:
- first_name: Victoria
full_name: Pokusaeva, Victoria
id: 3184041C-F248-11E8-B48F-1D18A9856A87
last_name: Pokusaeva
orcid: 0000-0001-7660-444X
- first_name: Aránzazu Rosado
full_name: Diez, Aránzazu Rosado
last_name: Diez
- first_name: Lorena
full_name: Espinar, Lorena
last_name: Espinar
- first_name: Albert Torelló
full_name: Pérez, Albert Torelló
last_name: Pérez
- first_name: Guillaume J.
full_name: Filion, Guillaume J.
last_name: Filion
citation:
ama: Pokusaeva V, Diez AR, Espinar L, Pérez AT, Filion GJ. Strand asymmetry influences
mismatch resolution during single-strand annealing. Genome Biology. 2022;23.
doi:10.1186/s13059-022-02665-3
apa: Pokusaeva, V., Diez, A. R., Espinar, L., Pérez, A. T., & Filion, G. J.
(2022). Strand asymmetry influences mismatch resolution during single-strand annealing.
Genome Biology. Springer Nature. https://doi.org/10.1186/s13059-022-02665-3
chicago: Pokusaeva, Victoria, Aránzazu Rosado Diez, Lorena Espinar, Albert Torelló
Pérez, and Guillaume J. Filion. “Strand Asymmetry Influences Mismatch Resolution
during Single-Strand Annealing.” Genome Biology. Springer Nature, 2022.
https://doi.org/10.1186/s13059-022-02665-3.
ieee: V. Pokusaeva, A. R. Diez, L. Espinar, A. T. Pérez, and G. J. Filion, “Strand
asymmetry influences mismatch resolution during single-strand annealing,” Genome
Biology, vol. 23. Springer Nature, 2022.
ista: Pokusaeva V, Diez AR, Espinar L, Pérez AT, Filion GJ. 2022. Strand asymmetry
influences mismatch resolution during single-strand annealing. Genome Biology.
23, 93.
mla: Pokusaeva, Victoria, et al. “Strand Asymmetry Influences Mismatch Resolution
during Single-Strand Annealing.” Genome Biology, vol. 23, 93, Springer
Nature, 2022, doi:10.1186/s13059-022-02665-3.
short: V. Pokusaeva, A.R. Diez, L. Espinar, A.T. Pérez, G.J. Filion, Genome Biology
23 (2022).
date_created: 2023-01-16T09:48:44Z
date_published: 2022-04-12T00:00:00Z
date_updated: 2023-08-04T09:27:00Z
day: '12'
ddc:
- '570'
department:
- _id: MaJö
doi: 10.1186/s13059-022-02665-3
ec_funded: 1
external_id:
isi:
- '000781953800001'
pmid:
- '35414014'
file:
- access_level: open_access
checksum: 17bb091fec04d82ba20a3458c4cfd2bd
content_type: application/pdf
creator: dernst
date_created: 2023-01-27T09:01:40Z
date_updated: 2023-01-27T09:01:40Z
file_id: '12419'
file_name: 2022_GenomeBiology_Pokusaeva.pdf
file_size: 4939342
relation: main_file
success: 1
file_date_updated: 2023-01-27T09:01:40Z
has_accepted_license: '1'
intvolume: ' 23'
isi: 1
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
publication: Genome Biology
publication_identifier:
issn:
- 1474-760X
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- relation: software
url: 'https://github.com/cellcomplexitylab/strand_asymmetry '
- relation: software
url: https://hub.docker.com/r/gui11aume/strand_asymmetry
scopus_import: '1'
status: public
title: Strand asymmetry influences mismatch resolution during single-strand annealing
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 23
year: '2022'
...
---
_id: '12288'
abstract:
- lang: eng
text: To understand the function of neuronal circuits, it is crucial to disentangle
the connectivity patterns within the network. However, most tools currently used
to explore connectivity have low throughput, low selectivity, or limited accessibility.
Here, we report the development of an improved packaging system for the production
of the highly neurotropic RVdGenvA-CVS-N2c rabies viral vectors, yielding titers
orders of magnitude higher with no background contamination, at a fraction of
the production time, while preserving the efficiency of transsynaptic labeling.
Along with the production pipeline, we developed suites of ‘starter’ AAV and bicistronic
RVdG-CVS-N2c vectors, enabling retrograde labeling from a wide range of neuronal
populations, tailored for diverse experimental requirements. We demonstrate the
power and flexibility of the new system by uncovering hidden local and distal
inhibitory connections in the mouse hippocampal formation and by imaging the functional
properties of a cortical microcircuit across weeks. Our novel production pipeline
provides a convenient approach to generate new rabies vectors, while our toolkit
flexibly and efficiently expands the current capacity to label, manipulate and
image the neuronal activity of interconnected neuronal circuits in vitro and in
vivo.
acknowledged_ssus:
- _id: Bio
- _id: PreCl
acknowledgement: We thank F Marr for technical assistance, A Murray for RVdG-CVS-N2c
viruses and Neuro2A packaging cell-lines and J Watson for reading the manuscript.
This research was supported by the Scientific Service Units (SSU) of IST-Austria
through resources provided by the Imaging and Optics Facility (IOF) and the Preclinical
Facility (PCF). This project was funded by the European Research Council (ERC) under
the European Union’s Horizon 2020 research and innovation programme (ERC advanced
grant No 692692, PJ, ERC starting grant No 756502, MJ), the Fond zur Förderung der
Wissenschaftlichen Forschung (Z 312-B27, Wittgenstein award, PJ), the Human Frontier
Science Program (LT000256/2018-L, AS) and EMBO (ALTF 1098-2017, AS).
article_number: '79848'
article_processing_charge: No
article_type: original
author:
- first_name: Anton L
full_name: Sumser, Anton L
id: 3320A096-F248-11E8-B48F-1D18A9856A87
last_name: Sumser
orcid: 0000-0002-4792-1881
- first_name: Maximilian A
full_name: Jösch, Maximilian A
id: 2BD278E6-F248-11E8-B48F-1D18A9856A87
last_name: Jösch
orcid: 0000-0002-3937-1330
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: Yoav
full_name: Ben Simon, Yoav
id: 43DF3136-F248-11E8-B48F-1D18A9856A87
last_name: Ben Simon
citation:
ama: Sumser AL, Jösch MA, Jonas PM, Ben Simon Y. Fast, high-throughput production
of improved rabies viral vectors for specific, efficient and versatile transsynaptic
retrograde labeling. eLife. 2022;11. doi:10.7554/elife.79848
apa: Sumser, A. L., Jösch, M. A., Jonas, P. M., & Ben Simon, Y. (2022). Fast,
high-throughput production of improved rabies viral vectors for specific, efficient
and versatile transsynaptic retrograde labeling. ELife. eLife Sciences
Publications. https://doi.org/10.7554/elife.79848
chicago: Sumser, Anton L, Maximilian A Jösch, Peter M Jonas, and Yoav Ben Simon.
“Fast, High-Throughput Production of Improved Rabies Viral Vectors for Specific,
Efficient and Versatile Transsynaptic Retrograde Labeling.” ELife. eLife
Sciences Publications, 2022. https://doi.org/10.7554/elife.79848.
ieee: A. L. Sumser, M. A. Jösch, P. M. Jonas, and Y. Ben Simon, “Fast, high-throughput
production of improved rabies viral vectors for specific, efficient and versatile
transsynaptic retrograde labeling,” eLife, vol. 11. eLife Sciences Publications,
2022.
ista: Sumser AL, Jösch MA, Jonas PM, Ben Simon Y. 2022. Fast, high-throughput production
of improved rabies viral vectors for specific, efficient and versatile transsynaptic
retrograde labeling. eLife. 11, 79848.
mla: Sumser, Anton L., et al. “Fast, High-Throughput Production of Improved Rabies
Viral Vectors for Specific, Efficient and Versatile Transsynaptic Retrograde Labeling.”
ELife, vol. 11, 79848, eLife Sciences Publications, 2022, doi:10.7554/elife.79848.
short: A.L. Sumser, M.A. Jösch, P.M. Jonas, Y. Ben Simon, ELife 11 (2022).
date_created: 2023-01-16T10:04:15Z
date_published: 2022-09-15T00:00:00Z
date_updated: 2023-08-04T10:29:48Z
day: '15'
ddc:
- '570'
department:
- _id: MaJö
- _id: PeJo
doi: 10.7554/elife.79848
ec_funded: 1
external_id:
isi:
- '000892204300001'
pmid:
- '36040301'
file:
- access_level: open_access
checksum: 5a2a65e3e7225090c3d8199f3bbd7b7b
content_type: application/pdf
creator: dernst
date_created: 2023-01-30T11:50:53Z
date_updated: 2023-01-30T11:50:53Z
file_id: '12463'
file_name: 2022_eLife_Sumser.pdf
file_size: 8506811
relation: main_file
success: 1
file_date_updated: 2023-01-30T11:50:53Z
has_accepted_license: '1'
intvolume: ' 11'
isi: 1
keyword:
- General Immunology and Microbiology
- General Biochemistry
- Genetics and Molecular Biology
- General Medicine
- General Neuroscience
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: 2634E9D2-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '756502'
name: Circuits of Visual Attention
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: The Wittgenstein Prize
- _id: 266D407A-B435-11E9-9278-68D0E5697425
grant_number: LT000256
name: Neuronal networks of salience and spatial detection in the murine superior
colliculus
- _id: 264FEA02-B435-11E9-9278-68D0E5697425
grant_number: ALTF 1098-2017
name: Connecting sensory with motor processing in the superior colliculus
publication: eLife
publication_identifier:
eissn:
- 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: Fast, high-throughput production of improved rabies viral vectors for specific,
efficient and versatile transsynaptic retrograde labeling
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 11
year: '2022'
...
---
_id: '9955'
abstract:
- lang: eng
text: Neurons can change their classical neurotransmitters during ontogeny, sometimes
going through stages of dual release. Here, we explored the development of the
neurotransmitter identity of neurons of the avian nucleus isthmi parvocellularis
(Ipc), whose axon terminals are retinotopically arranged in the optic tectum (TeO)
and exert a focal gating effect upon the ascending transmission of retinal inputs.
Although cholinergic and glutamatergic markers are both found in Ipc neurons and
terminals of adult pigeons and chicks, the mRNA expression of the vesicular acetylcholine
transporter, VAChT, is weak or absent. To explore how the Ipc neurotransmitter
identity is established during ontogeny, we analyzed the expression of mRNAs coding
for cholinergic (ChAT, VAChT, and CHT) and glutamatergic (VGluT2 and VGluT3) markers
in chick embryos at different developmental stages. We found that between E12
and E18, Ipc neurons expressed all cholinergic mRNAs and also VGluT2 mRNA; however,
from E16 through posthatch stages, VAChT mRNA expression was specifically diminished.
Our ex vivo deposits of tracer crystals and intracellular filling experiments
revealed that Ipc axons exhibit a mature paintbrush morphology late in development,
experiencing marked morphological transformations during the period of presumptive
dual vesicular transmitter release. Additionally, although ChAT protein immunoassays
increasingly label the growing Ipc axon, this labeling was consistently restricted
to sparse portions of the terminal branches. Combined, these results suggest that
the synthesis of glutamate and acetylcholine, and their vesicular release, is
complexly linked to the developmental processes of branching, growing and remodeling
of these unique axons.
acknowledgement: 'This work was supported by FONDECYT grants 1151432 and 1210169 to
Gonzalo J. Marín. FONDECYT grant 1210069 to Jorge Mpodozis. Spanish Ministry of
Science, Innovation and Universities (MCIU), State Research Agency (AEI) and European
Regional Development Fund (FEDER), PGC2018-098229-B-100 to José L Ferrán. Spanish
Ministry of Economy and Competitiveness Excellency Grant BFU2014-57516P (with European
Community FEDER support), and a Seneca Foundation (Autonomous Community of Murcia)
Excellency Research contract, ref: 19904/ GERM/15; project name: Genoarchitectonic
Brain Development and Applications to Neurodegenerative Diseases and Cancer (5672
Fundación Séneca) to Luis Puelles. The authors gratefully acknowledge the valuable
editorial help provided by Sara Fernández-Collemann. The authors also thank Elisa
Sentis and Solano Henríquez for expert technical help.'
article_processing_charge: No
article_type: original
author:
- first_name: Rosana
full_name: Reyes‐Pinto, Rosana
last_name: Reyes‐Pinto
- first_name: José L.
full_name: Ferrán, José L.
last_name: Ferrán
- first_name: Tomas A
full_name: Vega Zuniga, Tomas A
id: 2E7C4E78-F248-11E8-B48F-1D18A9856A87
last_name: Vega Zuniga
- first_name: Cristian
full_name: González‐Cabrera, Cristian
last_name: González‐Cabrera
- first_name: Harald
full_name: Luksch, Harald
last_name: Luksch
- first_name: Jorge
full_name: Mpodozis, Jorge
last_name: Mpodozis
- first_name: Luis
full_name: Puelles, Luis
last_name: Puelles
- first_name: Gonzalo J.
full_name: Marín, Gonzalo J.
last_name: Marín
citation:
ama: Reyes‐Pinto R, Ferrán JL, Vega Zuniga TA, et al. Change in the neurochemical
signature and morphological development of the parvocellular isthmic projection
to the avian tectum. Journal of Comparative Neurology. 2022;530(2):553-573.
doi:10.1002/cne.25229
apa: Reyes‐Pinto, R., Ferrán, J. L., Vega Zuniga, T. A., González‐Cabrera, C., Luksch,
H., Mpodozis, J., … Marín, G. J. (2022). Change in the neurochemical signature
and morphological development of the parvocellular isthmic projection to the avian
tectum. Journal of Comparative Neurology. Wiley. https://doi.org/10.1002/cne.25229
chicago: Reyes‐Pinto, Rosana, José L. Ferrán, Tomas A Vega Zuniga, Cristian González‐Cabrera,
Harald Luksch, Jorge Mpodozis, Luis Puelles, and Gonzalo J. Marín. “Change in
the Neurochemical Signature and Morphological Development of the Parvocellular
Isthmic Projection to the Avian Tectum.” Journal of Comparative Neurology.
Wiley, 2022. https://doi.org/10.1002/cne.25229.
ieee: R. Reyes‐Pinto et al., “Change in the neurochemical signature and morphological
development of the parvocellular isthmic projection to the avian tectum,” Journal
of Comparative Neurology, vol. 530, no. 2. Wiley, pp. 553–573, 2022.
ista: Reyes‐Pinto R, Ferrán JL, Vega Zuniga TA, González‐Cabrera C, Luksch H, Mpodozis
J, Puelles L, Marín GJ. 2022. Change in the neurochemical signature and morphological
development of the parvocellular isthmic projection to the avian tectum. Journal
of Comparative Neurology. 530(2), 553–573.
mla: Reyes‐Pinto, Rosana, et al. “Change in the Neurochemical Signature and Morphological
Development of the Parvocellular Isthmic Projection to the Avian Tectum.” Journal
of Comparative Neurology, vol. 530, no. 2, Wiley, 2022, pp. 553–73, doi:10.1002/cne.25229.
short: R. Reyes‐Pinto, J.L. Ferrán, T.A. Vega Zuniga, C. González‐Cabrera, H. Luksch,
J. Mpodozis, L. Puelles, G.J. Marín, Journal of Comparative Neurology 530 (2022)
553–573.
date_created: 2021-08-23T08:40:59Z
date_published: 2022-02-01T00:00:00Z
date_updated: 2023-08-11T10:58:17Z
day: '01'
department:
- _id: MaJö
doi: 10.1002/cne.25229
external_id:
isi:
- '000686420000001'
pmid:
- '34363623'
intvolume: ' 530'
isi: 1
issue: '2'
language:
- iso: eng
month: '02'
oa_version: None
page: 553-573
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
eissn:
- 1096-9861
issn:
- 0021-9967
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Change in the neurochemical signature and morphological development of the
parvocellular isthmic projection to the avian tectum
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 530
year: '2022'
...
---
_id: '7551'
abstract:
- lang: eng
text: Novelty facilitates formation of memories. The detection of novelty and storage
of contextual memories are both mediated by the hippocampus, yet the mechanisms
that link these two functions remain to be defined. Dentate granule cells (GCs)
of the dorsal hippocampus fire upon novelty exposure forming engrams of contextual
memory. However, their key excitatory inputs from the entorhinal cortex are not
responsive to novelty and are insufficient to make dorsal GCs fire reliably. Here
we uncover a powerful glutamatergic pathway to dorsal GCs from ventral hippocampal
mossy cells (MCs) that relays novelty, and is necessary and sufficient for driving
dorsal GCs activation. Furthermore, manipulation of ventral MCs activity bidirectionally
regulates novelty-induced contextual memory acquisition. Our results show that
ventral MCs activity controls memory formation through an intra-hippocampal interaction
mechanism gated by novelty.
acknowledgement: We thank Peter Jonas and Peter Somogyi for critically reading the
manuscript, Satoshi Kida for helpful discussion, Taijia Makinen for providing the
Prox1-creERT2 mouse line, and Hiromu Yawo for the VAMP2-Venus construct. We also
thank Vivek Jayaraman, Ph.D.; Rex A. Kerr, Ph.D.; Douglas S. Kim, Ph.D.; Loren L.
Looger, Ph.D.; and Karel Svoboda, Ph.D. from the GENIE Project, Janelia Farm Research
Campus, Howard Hughes Medical Institute for the viral constructs used for GCaMP6s
expression. We also thank Jacqueline Montanaro, Vanessa Zheden, David Kleindienst,
and Laura Burnett for technical assistance, as well as Robert Beattie for imaging
assistance. This work was supported by a European Research Council Advanced Grant
694539 to R.S.
article_processing_charge: No
article_type: original
author:
- first_name: Felipe A
full_name: Fredes Tolorza, Felipe A
id: 384825DA-F248-11E8-B48F-1D18A9856A87
last_name: Fredes Tolorza
- first_name: Maria A
full_name: Silva Sifuentes, Maria A
id: 371B3D6E-F248-11E8-B48F-1D18A9856A87
last_name: Silva Sifuentes
- first_name: Peter
full_name: Koppensteiner, Peter
id: 3B8B25A8-F248-11E8-B48F-1D18A9856A87
last_name: Koppensteiner
- first_name: Kenta
full_name: Kobayashi, Kenta
last_name: Kobayashi
- first_name: Maximilian A
full_name: Jösch, Maximilian A
id: 2BD278E6-F248-11E8-B48F-1D18A9856A87
last_name: Jösch
orcid: 0000-0002-3937-1330
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
citation:
ama: Fredes Tolorza FA, Silva Sifuentes MA, Koppensteiner P, Kobayashi K, Jösch
MA, Shigemoto R. Ventro-dorsal hippocampal pathway gates novelty-induced contextual
memory formation. Current Biology. 2021;31(1):P25-38.E5. doi:10.1016/j.cub.2020.09.074
apa: Fredes Tolorza, F. A., Silva Sifuentes, M. A., Koppensteiner, P., Kobayashi,
K., Jösch, M. A., & Shigemoto, R. (2021). Ventro-dorsal hippocampal pathway
gates novelty-induced contextual memory formation. Current Biology. Elsevier.
https://doi.org/10.1016/j.cub.2020.09.074
chicago: Fredes Tolorza, Felipe A, Maria A Silva Sifuentes, Peter Koppensteiner,
Kenta Kobayashi, Maximilian A Jösch, and Ryuichi Shigemoto. “Ventro-Dorsal Hippocampal
Pathway Gates Novelty-Induced Contextual Memory Formation.” Current Biology.
Elsevier, 2021. https://doi.org/10.1016/j.cub.2020.09.074.
ieee: F. A. Fredes Tolorza, M. A. Silva Sifuentes, P. Koppensteiner, K. Kobayashi,
M. A. Jösch, and R. Shigemoto, “Ventro-dorsal hippocampal pathway gates novelty-induced
contextual memory formation,” Current Biology, vol. 31, no. 1. Elsevier,
p. P25–38.E5, 2021.
ista: Fredes Tolorza FA, Silva Sifuentes MA, Koppensteiner P, Kobayashi K, Jösch
MA, Shigemoto R. 2021. Ventro-dorsal hippocampal pathway gates novelty-induced
contextual memory formation. Current Biology. 31(1), P25–38.E5.
mla: Fredes Tolorza, Felipe A., et al. “Ventro-Dorsal Hippocampal Pathway Gates
Novelty-Induced Contextual Memory Formation.” Current Biology, vol. 31,
no. 1, Elsevier, 2021, p. P25–38.E5, doi:10.1016/j.cub.2020.09.074.
short: F.A. Fredes Tolorza, M.A. Silva Sifuentes, P. Koppensteiner, K. Kobayashi,
M.A. Jösch, R. Shigemoto, Current Biology 31 (2021) P25–38.E5.
date_created: 2020-02-28T10:56:18Z
date_published: 2021-01-11T00:00:00Z
date_updated: 2023-08-04T10:47:11Z
day: '11'
ddc:
- '570'
department:
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- _id: RySh
doi: 10.1016/j.cub.2020.09.074
ec_funded: 1
external_id:
isi:
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oa_version: Published Version
page: P25-38.E5
project:
- _id: 25CA28EA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '694539'
name: 'In situ analysis of single channel subunit composition in neurons: physiological
implication in synaptic plasticity and behaviour'
publication: Current Biology
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/remembering-novelty/
status: public
title: Ventro-dorsal hippocampal pathway gates novelty-induced contextual memory formation
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
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...
---
_id: '8643'
abstract:
- lang: eng
text: The parabigeminal nucleus (PBG) is the mammalian homologue to the isthmic
complex of other vertebrates. Optogenetic stimulation of the PBG induces freezing
and escape in mice, a result thought to be caused by a PBG projection to the central
nucleus of the amygdala. However, the isthmic complex, including the PBG, has
been classically considered satellite nuclei of the Superior Colliculus (SC),
which upon stimulation of its medial part also triggers fear and avoidance reactions.
As the PBG-SC connectivity is not well characterized, we investigated whether
the topology of the PBG projection to the SC could be related to the behavioral
consequences of PBG stimulation. To that end, we performed immunohistochemistry,
in situ hybridization and neural tracer injections in the SC and PBG in a diurnal
rodent, the Octodon degus. We found that all PBG neurons expressed both glutamatergic
and cholinergic markers and were distributed in clearly defined anterior (aPBG)
and posterior (pPBG) subdivisions. The pPBG is connected reciprocally and topographically
to the ipsilateral SC, whereas the aPBG receives afferent axons from the ipsilateral
SC and projected exclusively to the contralateral SC. This contralateral projection
forms a dense field of terminals that is restricted to the medial SC, in correspondence
with the SC representation of the aerial binocular field which, we also found,
in O. degus prompted escape reactions upon looming stimulation. Therefore, this
specialized topography allows binocular interactions in the SC region controlling
responses to aerial predators, suggesting a link between the mechanisms by which
the SC and PBG produce defensive behaviors.
acknowledgement: 'We thank Elisa Sentis and Solano Henriquez for their expert technical
assistance. Dr. David Sterratt for his helpful advice in using the Retistruct package.
Dr. Joao Botelho for his valuable assistance in scanning the retinas. To Mrs. Diane
Greenstein for kindly reading and correcting our manuscript. Macarena Ruiz for her
helpful comments during figures elaboration. Dr. Alexia Nunez-Parra for kindly providing
us with the transgenic mouse line. Dr. Harald Luksch for granting us access to the
confocal microscope at his lab. This study was supported by: FONDECYT 1151432 (to
G.M.), FONDECYT 1170027 (to J.M.) and Doctoral fellowship CONICYT 21161599 (to A.D.).'
article_number: '16220'
article_processing_charge: No
article_type: original
author:
- first_name: Alfonso
full_name: Deichler, Alfonso
last_name: Deichler
- first_name: Denisse
full_name: Carrasco, Denisse
last_name: Carrasco
- first_name: Luciana
full_name: Lopez-Jury, Luciana
last_name: Lopez-Jury
- first_name: Tomas A
full_name: Vega Zuniga, Tomas A
id: 2E7C4E78-F248-11E8-B48F-1D18A9856A87
last_name: Vega Zuniga
- first_name: Natalia
full_name: Marquez, Natalia
last_name: Marquez
- first_name: Jorge
full_name: Mpodozis, Jorge
last_name: Mpodozis
- first_name: Gonzalo
full_name: Marin, Gonzalo
last_name: Marin
citation:
ama: Deichler A, Carrasco D, Lopez-Jury L, et al. A specialized reciprocal connectivity
suggests a link between the mechanisms by which the superior colliculus and parabigeminal
nucleus produce defensive behaviors in rodents. Scientific Reports. 2020;10.
doi:10.1038/s41598-020-72848-0
apa: Deichler, A., Carrasco, D., Lopez-Jury, L., Vega Zuniga, T. A., Marquez, N.,
Mpodozis, J., & Marin, G. (2020). A specialized reciprocal connectivity suggests
a link between the mechanisms by which the superior colliculus and parabigeminal
nucleus produce defensive behaviors in rodents. Scientific Reports. Springer
Nature. https://doi.org/10.1038/s41598-020-72848-0
chicago: Deichler, Alfonso, Denisse Carrasco, Luciana Lopez-Jury, Tomas A Vega Zuniga,
Natalia Marquez, Jorge Mpodozis, and Gonzalo Marin. “A Specialized Reciprocal
Connectivity Suggests a Link between the Mechanisms by Which the Superior Colliculus
and Parabigeminal Nucleus Produce Defensive Behaviors in Rodents.” Scientific
Reports. Springer Nature, 2020. https://doi.org/10.1038/s41598-020-72848-0.
ieee: A. Deichler et al., “A specialized reciprocal connectivity suggests
a link between the mechanisms by which the superior colliculus and parabigeminal
nucleus produce defensive behaviors in rodents,” Scientific Reports, vol.
10. Springer Nature, 2020.
ista: Deichler A, Carrasco D, Lopez-Jury L, Vega Zuniga TA, Marquez N, Mpodozis
J, Marin G. 2020. A specialized reciprocal connectivity suggests a link between
the mechanisms by which the superior colliculus and parabigeminal nucleus produce
defensive behaviors in rodents. Scientific Reports. 10, 16220.
mla: Deichler, Alfonso, et al. “A Specialized Reciprocal Connectivity Suggests a
Link between the Mechanisms by Which the Superior Colliculus and Parabigeminal
Nucleus Produce Defensive Behaviors in Rodents.” Scientific Reports, vol.
10, 16220, Springer Nature, 2020, doi:10.1038/s41598-020-72848-0.
short: A. Deichler, D. Carrasco, L. Lopez-Jury, T.A. Vega Zuniga, N. Marquez, J.
Mpodozis, G. Marin, Scientific Reports 10 (2020).
date_created: 2020-10-11T22:01:14Z
date_published: 2020-10-01T00:00:00Z
date_updated: 2023-08-22T09:58:21Z
day: '01'
ddc:
- '570'
department:
- _id: MaJö
doi: 10.1038/s41598-020-72848-0
external_id:
isi:
- '000577142600032'
file:
- access_level: open_access
checksum: f6dd99954f1c0ffb4da5a1d2d739bf31
content_type: application/pdf
creator: dernst
date_created: 2020-10-12T12:39:10Z
date_updated: 2020-10-12T12:39:10Z
file_id: '8651'
file_name: 2020_ScientificReport_Deichler.pdf
file_size: 3906744
relation: main_file
success: 1
file_date_updated: 2020-10-12T12:39:10Z
has_accepted_license: '1'
intvolume: ' 10'
isi: 1
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
publication: Scientific Reports
publication_identifier:
eissn:
- '20452322'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: A specialized reciprocal connectivity suggests a link between the mechanisms
by which the superior colliculus and parabigeminal nucleus produce defensive behaviors
in rodents
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 10
year: '2020'
...