[{"type":"dissertation","tmp":{"name":"Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)","image":"/images/cc_by_nc_sa.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode","short":"CC BY-NC-SA (4.0)"},"status":"public","_id":"12531","department":[{"_id":"GradSch"},{"_id":"MaJö"}],"file_date_updated":"2024-02-09T23:30:03Z","supervisor":[{"id":"2BD278E6-F248-11E8-B48F-1D18A9856A87","first_name":"Maximilian A","last_name":"Jösch","full_name":"Jösch, Maximilian A","orcid":"0000-0002-3937-1330"}],"date_updated":"2024-02-09T23:30:04Z","ddc":["570"],"alternative_title":["ISTA Master's Thesis"],"month":"02","abstract":[{"lang":"eng","text":"All visual experiences of the vertebrates begin with light being converted into electrical signals\r\nby the eye retina. Retinal ganglion cells (RGCs) are the neurons of the innermost layer of the\r\nmammal retina, and they transmit visual information to the rest of the brain.\r\nIt has been shown that RGCs vary in their morphology and genetic profiles, moreover they can\r\nbe unambiguously grouped into subtypes that share the same morphological and/or molecular\r\nproperties. However, in terms of RGCs function, it remains unclear how many distinct types\r\nthere are and what response properties their typology relies on. Even given the recent studies\r\nthat successfully classified RGCs in a patch of the retina [1] and in scotopic conditions [2], the\r\nquestion remains whether the found subtypes persist across the entire retina.\r\nIn this work, using a novel imaging method, we show that, when sampled from a large portion\r\nof the retina, RGCs can not be clearly divided into functional subtypes. We found that in\r\nphotopic conditions, which implies more prominent natural scene statistic differences across\r\nthe visual field, response properties can be exhibited by cells differently depending on their\r\nlocation in the retina, which leads to formation of a gradient of features rather than distinct\r\nclasses.\r\nThis finding suggests that RGCs follow a global organization across the visual field of the\r\nanimal, adapting each RGC subtype to the requirements imposed by the natural scene statistics."}],"oa_version":"Published Version","license":"https://creativecommons.org/licenses/by-nc-sa/4.0/","publication_identifier":{"issn":["2791-4585"]},"degree_awarded":"MS","publication_status":"published","file":[{"checksum":"57d8da3a6c749eb1556b7435fe266a5f","file_id":"12532","embargo":"2024-02-08","access_level":"open_access","relation":"main_file","content_type":"application/pdf","date_created":"2023-02-09T08:03:32Z","file_name":"Thesis_Kseniia___ISTA__istaustriathesis_PDF-A.pdf","creator":"cchlebak","date_updated":"2024-02-09T23:30:03Z","file_size":8369317},{"content_type":"application/x-zip-compressed","embargo_to":"open_access","access_level":"closed","relation":"source_file","checksum":"87fb44318e4f9eb9da2ad9ad6ca8e76f","file_id":"12535","date_updated":"2024-02-09T23:30:03Z","file_size":11204408,"creator":"cchlebak","date_created":"2023-02-10T09:32:06Z","file_name":"Thesis Kseniia - ISTA [istaustriathesis]-FINAL.zip"}],"language":[{"iso":"eng"}],"author":[{"id":"8e3f931e-dc85-11ea-9058-e7b957bf23f0","first_name":"Kseniia","full_name":"Kirillova, Kseniia","last_name":"Kirillova"}],"article_processing_charge":"No","title":"Panoramic functional gradients across the mouse retina","citation":{"chicago":"Kirillova, Kseniia. “Panoramic Functional Gradients across the Mouse Retina.” Institute of Science and Technology Austria, 2023. https://doi.org/10.15479/at:ista:12531.","ista":"Kirillova K. 2023. Panoramic functional gradients across the mouse retina. Institute of Science and Technology Austria.","mla":"Kirillova, Kseniia. Panoramic Functional Gradients across the Mouse Retina. Institute of Science and Technology Austria, 2023, doi:10.15479/at:ista:12531.","ieee":"K. Kirillova, “Panoramic functional gradients across the mouse retina,” Institute of Science and Technology Austria, 2023.","short":"K. Kirillova, Panoramic Functional Gradients across the Mouse Retina, Institute of Science and Technology Austria, 2023.","apa":"Kirillova, K. (2023). Panoramic functional gradients across the mouse retina. Institute of Science and Technology Austria. https://doi.org/10.15479/at:ista:12531","ama":"Kirillova K. Panoramic functional gradients across the mouse retina. 2023. doi:10.15479/at:ista:12531"},"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publisher":"Institute of Science and Technology Austria","oa":1,"page":"46","doi":"10.15479/at:ista:12531","date_published":"2023-02-08T00:00:00Z","date_created":"2023-02-09T07:45:05Z","has_accepted_license":"1","year":"2023","day":"08"},{"oa":1,"publisher":"Springer Nature","quality_controlled":"1","acknowledgement":"We acknowledge the financial support of the Natural Sciences and Engineering Research Council of Canada (NSERC RGPIN-2020-06377), the Spanish Ministry of Economy, Industry and Competitiveness (“Centro de Excelencia Severo Ochoa 2013-2017”, Plan Estatal PGC2018-099807-B-I00), of the CERCA Programme/Generalitat de Catalunya, and of the European Research Council (Synergy Grant 609989). VOP was supported by the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie programme (665385). We also acknowledge the support of the Spanish Ministry of Economy and Competitiveness (MEIC) to the EMBL partnership.","date_created":"2023-01-16T09:48:44Z","date_published":"2022-04-12T00:00:00Z","doi":"10.1186/s13059-022-02665-3","year":"2022","has_accepted_license":"1","isi":1,"publication":"Genome Biology","day":"12","project":[{"grant_number":"665385","name":"International IST Doctoral Program","call_identifier":"H2020","_id":"2564DBCA-B435-11E9-9278-68D0E5697425"}],"article_number":"93","article_processing_charge":"No","external_id":{"pmid":["35414014"],"isi":["000781953800001"]},"author":[{"full_name":"Pokusaeva, Victoria","orcid":"0000-0001-7660-444X","last_name":"Pokusaeva","first_name":"Victoria","id":"3184041C-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Diez","full_name":"Diez, Aránzazu Rosado","first_name":"Aránzazu Rosado"},{"first_name":"Lorena","last_name":"Espinar","full_name":"Espinar, Lorena"},{"last_name":"Pérez","full_name":"Pérez, Albert Torelló","first_name":"Albert Torelló"},{"last_name":"Filion","full_name":"Filion, Guillaume J.","first_name":"Guillaume J."}],"title":"Strand asymmetry influences mismatch resolution during single-strand annealing","citation":{"short":"V. Pokusaeva, A.R. Diez, L. Espinar, A.T. Pérez, G.J. Filion, Genome Biology 23 (2022).","ieee":"V. Pokusaeva, A. R. Diez, L. Espinar, A. T. Pérez, and G. J. Filion, “Strand asymmetry influences mismatch resolution during single-strand annealing,” Genome Biology, vol. 23. Springer Nature, 2022.","apa":"Pokusaeva, V., Diez, A. R., Espinar, L., Pérez, A. T., & Filion, G. J. (2022). Strand asymmetry influences mismatch resolution during single-strand annealing. Genome Biology. Springer Nature. https://doi.org/10.1186/s13059-022-02665-3","ama":"Pokusaeva V, Diez AR, Espinar L, Pérez AT, Filion GJ. Strand asymmetry influences mismatch resolution during single-strand annealing. Genome Biology. 2022;23. doi:10.1186/s13059-022-02665-3","mla":"Pokusaeva, Victoria, et al. “Strand Asymmetry Influences Mismatch Resolution during Single-Strand Annealing.” Genome Biology, vol. 23, 93, Springer Nature, 2022, doi:10.1186/s13059-022-02665-3.","ista":"Pokusaeva V, Diez AR, Espinar L, Pérez AT, Filion GJ. 2022. Strand asymmetry influences mismatch resolution during single-strand annealing. Genome Biology. 23, 93.","chicago":"Pokusaeva, Victoria, Aránzazu Rosado Diez, Lorena Espinar, Albert Torelló Pérez, and Guillaume J. Filion. “Strand Asymmetry Influences Mismatch Resolution during Single-Strand Annealing.” Genome Biology. Springer Nature, 2022. https://doi.org/10.1186/s13059-022-02665-3."},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","scopus_import":"1","intvolume":" 23","month":"04","abstract":[{"text":"Background: Biases of DNA repair can shape the nucleotide landscape of genomes at evolutionary timescales. The molecular mechanisms of those biases are still poorly understood because it is difficult to isolate the contributions of DNA repair from those of DNA damage.\r\n\r\nResults: Here, we develop a genome-wide assay whereby the same DNA lesion is repaired in different genomic contexts. We insert thousands of barcoded transposons carrying a reporter of DNA mismatch repair in the genome of mouse embryonic stem cells. Upon inducing a double-strand break between tandem repeats, a mismatch is generated if the break is repaired through single-strand annealing. The resolution of the mismatch showed a 60–80% bias in favor of the strand with the longest 3′ flap. The location of the lesion in the genome and the type of mismatch had little influence on the bias. Instead, we observe a complete reversal of the bias when the longest 3′ flap is moved to the opposite strand by changing the position of the double-strand break in the reporter.\r\n\r\nConclusions: These results suggest that the processing of the double-strand break has a major influence on the repair of mismatches during single-strand annealing.","lang":"eng"}],"pmid":1,"oa_version":"Published Version","ec_funded":1,"related_material":{"link":[{"url":"https://github.com/cellcomplexitylab/strand_asymmetry ","relation":"software"},{"url":"https://hub.docker.com/r/gui11aume/strand_asymmetry","relation":"software"}]},"volume":23,"publication_status":"published","publication_identifier":{"issn":["1474-760X"]},"language":[{"iso":"eng"}],"file":[{"creator":"dernst","date_updated":"2023-01-27T09:01:40Z","file_size":4939342,"date_created":"2023-01-27T09:01:40Z","file_name":"2022_GenomeBiology_Pokusaeva.pdf","access_level":"open_access","relation":"main_file","content_type":"application/pdf","file_id":"12419","checksum":"17bb091fec04d82ba20a3458c4cfd2bd","success":1}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"type":"journal_article","article_type":"original","status":"public","_id":"12226","department":[{"_id":"MaJö"}],"file_date_updated":"2023-01-27T09:01:40Z","date_updated":"2023-08-04T09:27:00Z","ddc":["570"]},{"acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"}],"abstract":[{"text":"To understand the function of neuronal circuits, it is crucial to disentangle the connectivity patterns within the network. However, most tools currently used to explore connectivity have low throughput, low selectivity, or limited accessibility. Here, we report the development of an improved packaging system for the production of the highly neurotropic RVdGenvA-CVS-N2c rabies viral vectors, yielding titers orders of magnitude higher with no background contamination, at a fraction of the production time, while preserving the efficiency of transsynaptic labeling. Along with the production pipeline, we developed suites of ‘starter’ AAV and bicistronic RVdG-CVS-N2c vectors, enabling retrograde labeling from a wide range of neuronal populations, tailored for diverse experimental requirements. We demonstrate the power and flexibility of the new system by uncovering hidden local and distal inhibitory connections in the mouse hippocampal formation and by imaging the functional properties of a cortical microcircuit across weeks. Our novel production pipeline provides a convenient approach to generate new rabies vectors, while our toolkit flexibly and efficiently expands the current capacity to label, manipulate and image the neuronal activity of interconnected neuronal circuits in vitro and in vivo.","lang":"eng"}],"oa_version":"Published Version","pmid":1,"scopus_import":"1","intvolume":" 11","month":"09","publication_status":"published","publication_identifier":{"eissn":["2050-084X"]},"language":[{"iso":"eng"}],"file":[{"creator":"dernst","date_updated":"2023-01-30T11:50:53Z","file_size":8506811,"date_created":"2023-01-30T11:50:53Z","file_name":"2022_eLife_Sumser.pdf","access_level":"open_access","relation":"main_file","content_type":"application/pdf","checksum":"5a2a65e3e7225090c3d8199f3bbd7b7b","file_id":"12463","success":1}],"ec_funded":1,"volume":11,"_id":"12288","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"type":"journal_article","article_type":"original","keyword":["General Immunology and Microbiology","General Biochemistry","Genetics and Molecular Biology","General Medicine","General Neuroscience"],"status":"public","date_updated":"2023-08-04T10:29:48Z","ddc":["570"],"file_date_updated":"2023-01-30T11:50:53Z","department":[{"_id":"MaJö"},{"_id":"PeJo"}],"acknowledgement":"We thank F Marr for technical assistance, A Murray for RVdG-CVS-N2c viruses and Neuro2A packaging cell-lines and J Watson for reading the manuscript. This research was supported by the Scientific Service Units (SSU) of IST-Austria through resources provided by the Imaging and Optics Facility (IOF) and the Preclinical Facility (PCF). This project was funded by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (ERC advanced grant No 692692, PJ, ERC starting grant No 756502, MJ), the Fond zur Förderung der Wissenschaftlichen Forschung (Z 312-B27, Wittgenstein award, PJ), the Human Frontier Science Program (LT000256/2018-L, AS) and EMBO (ALTF 1098-2017, AS).","oa":1,"quality_controlled":"1","publisher":"eLife Sciences Publications","year":"2022","has_accepted_license":"1","isi":1,"publication":"eLife","day":"15","date_created":"2023-01-16T10:04:15Z","date_published":"2022-09-15T00:00:00Z","doi":"10.7554/elife.79848","article_number":"79848","project":[{"_id":"25B7EB9E-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"692692","name":"Biophysics and circuit function of a giant cortical glumatergic synapse"},{"call_identifier":"H2020","_id":"2634E9D2-B435-11E9-9278-68D0E5697425","grant_number":"756502","name":"Circuits of Visual Attention"},{"call_identifier":"FWF","_id":"25C5A090-B435-11E9-9278-68D0E5697425","name":"The Wittgenstein Prize","grant_number":"Z00312"},{"grant_number":"LT000256","name":"Neuronal networks of salience and spatial detection in the murine superior colliculus","_id":"266D407A-B435-11E9-9278-68D0E5697425"},{"grant_number":"ALTF 1098-2017","name":"Connecting sensory with motor processing in the superior colliculus","_id":"264FEA02-B435-11E9-9278-68D0E5697425"}],"citation":{"chicago":"Sumser, Anton L, Maximilian A Jösch, Peter M Jonas, and Yoav Ben Simon. “Fast, High-Throughput Production of Improved Rabies Viral Vectors for Specific, Efficient and Versatile Transsynaptic Retrograde Labeling.” ELife. eLife Sciences Publications, 2022. https://doi.org/10.7554/elife.79848.","ista":"Sumser AL, Jösch MA, Jonas PM, Ben Simon Y. 2022. Fast, high-throughput production of improved rabies viral vectors for specific, efficient and versatile transsynaptic retrograde labeling. eLife. 11, 79848.","mla":"Sumser, Anton L., et al. “Fast, High-Throughput Production of Improved Rabies Viral Vectors for Specific, Efficient and Versatile Transsynaptic Retrograde Labeling.” ELife, vol. 11, 79848, eLife Sciences Publications, 2022, doi:10.7554/elife.79848.","short":"A.L. Sumser, M.A. Jösch, P.M. Jonas, Y. Ben Simon, ELife 11 (2022).","ieee":"A. L. Sumser, M. A. Jösch, P. M. Jonas, and Y. Ben Simon, “Fast, high-throughput production of improved rabies viral vectors for specific, efficient and versatile transsynaptic retrograde labeling,” eLife, vol. 11. eLife Sciences Publications, 2022.","apa":"Sumser, A. L., Jösch, M. A., Jonas, P. M., & Ben Simon, Y. (2022). Fast, high-throughput production of improved rabies viral vectors for specific, efficient and versatile transsynaptic retrograde labeling. ELife. eLife Sciences Publications. https://doi.org/10.7554/elife.79848","ama":"Sumser AL, Jösch MA, Jonas PM, Ben Simon Y. Fast, high-throughput production of improved rabies viral vectors for specific, efficient and versatile transsynaptic retrograde labeling. eLife. 2022;11. doi:10.7554/elife.79848"},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","article_processing_charge":"No","external_id":{"isi":["000892204300001"],"pmid":["36040301"]},"author":[{"full_name":"Sumser, Anton L","orcid":"0000-0002-4792-1881","last_name":"Sumser","id":"3320A096-F248-11E8-B48F-1D18A9856A87","first_name":"Anton L"},{"last_name":"Jösch","orcid":"0000-0002-3937-1330","full_name":"Jösch, Maximilian A","id":"2BD278E6-F248-11E8-B48F-1D18A9856A87","first_name":"Maximilian A"},{"last_name":"Jonas","orcid":"0000-0001-5001-4804","full_name":"Jonas, Peter M","id":"353C1B58-F248-11E8-B48F-1D18A9856A87","first_name":"Peter M"},{"id":"43DF3136-F248-11E8-B48F-1D18A9856A87","first_name":"Yoav","full_name":"Ben Simon, Yoav","last_name":"Ben Simon"}],"title":"Fast, high-throughput production of improved rabies viral vectors for specific, efficient and versatile transsynaptic retrograde labeling"},{"date_created":"2021-08-23T08:40:59Z","date_published":"2022-02-01T00:00:00Z","doi":"10.1002/cne.25229","page":"553-573","publication":"Journal of Comparative Neurology","day":"01","year":"2022","isi":1,"quality_controlled":"1","publisher":"Wiley","acknowledgement":"This work was supported by FONDECYT grants 1151432 and 1210169 to Gonzalo J. Marín. FONDECYT grant 1210069 to Jorge Mpodozis. Spanish Ministry of Science, Innovation and Universities (MCIU), State Research Agency (AEI) and European Regional Development Fund (FEDER), PGC2018-098229-B-100 to José L Ferrán. Spanish Ministry of Economy and Competitiveness Excellency Grant BFU2014-57516P (with European Community FEDER support), and a Seneca Foundation (Autonomous Community of Murcia) Excellency Research contract, ref: 19904/ GERM/15; project name: Genoarchitectonic Brain Development and Applications to Neurodegenerative Diseases and Cancer (5672 Fundación Séneca) to Luis Puelles. The authors gratefully acknowledge the valuable editorial help provided by Sara Fernández-Collemann. The authors also thank Elisa Sentis and Solano Henríquez for expert technical help.","title":"Change in the neurochemical signature and morphological development of the parvocellular isthmic projection to the avian tectum","article_processing_charge":"No","external_id":{"pmid":["34363623"],"isi":["000686420000001"]},"author":[{"first_name":"Rosana","last_name":"Reyes‐Pinto","full_name":"Reyes‐Pinto, Rosana"},{"first_name":"José L.","full_name":"Ferrán, José L.","last_name":"Ferrán"},{"first_name":"Tomas A","id":"2E7C4E78-F248-11E8-B48F-1D18A9856A87","full_name":"Vega Zuniga, Tomas A","last_name":"Vega Zuniga"},{"full_name":"González‐Cabrera, Cristian","last_name":"González‐Cabrera","first_name":"Cristian"},{"full_name":"Luksch, Harald","last_name":"Luksch","first_name":"Harald"},{"first_name":"Jorge","last_name":"Mpodozis","full_name":"Mpodozis, Jorge"},{"full_name":"Puelles, Luis","last_name":"Puelles","first_name":"Luis"},{"first_name":"Gonzalo J.","full_name":"Marín, Gonzalo J.","last_name":"Marín"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"short":"R. Reyes‐Pinto, J.L. Ferrán, T.A. Vega Zuniga, C. González‐Cabrera, H. Luksch, J. Mpodozis, L. Puelles, G.J. Marín, Journal of Comparative Neurology 530 (2022) 553–573.","ieee":"R. Reyes‐Pinto et al., “Change in the neurochemical signature and morphological development of the parvocellular isthmic projection to the avian tectum,” Journal of Comparative Neurology, vol. 530, no. 2. Wiley, pp. 553–573, 2022.","ama":"Reyes‐Pinto R, Ferrán JL, Vega Zuniga TA, et al. Change in the neurochemical signature and morphological development of the parvocellular isthmic projection to the avian tectum. Journal of Comparative Neurology. 2022;530(2):553-573. doi:10.1002/cne.25229","apa":"Reyes‐Pinto, R., Ferrán, J. L., Vega Zuniga, T. A., González‐Cabrera, C., Luksch, H., Mpodozis, J., … Marín, G. J. (2022). Change in the neurochemical signature and morphological development of the parvocellular isthmic projection to the avian tectum. Journal of Comparative Neurology. Wiley. https://doi.org/10.1002/cne.25229","mla":"Reyes‐Pinto, Rosana, et al. “Change in the Neurochemical Signature and Morphological Development of the Parvocellular Isthmic Projection to the Avian Tectum.” Journal of Comparative Neurology, vol. 530, no. 2, Wiley, 2022, pp. 553–73, doi:10.1002/cne.25229.","ista":"Reyes‐Pinto R, Ferrán JL, Vega Zuniga TA, González‐Cabrera C, Luksch H, Mpodozis J, Puelles L, Marín GJ. 2022. Change in the neurochemical signature and morphological development of the parvocellular isthmic projection to the avian tectum. Journal of Comparative Neurology. 530(2), 553–573.","chicago":"Reyes‐Pinto, Rosana, José L. Ferrán, Tomas A Vega Zuniga, Cristian González‐Cabrera, Harald Luksch, Jorge Mpodozis, Luis Puelles, and Gonzalo J. Marín. “Change in the Neurochemical Signature and Morphological Development of the Parvocellular Isthmic Projection to the Avian Tectum.” Journal of Comparative Neurology. Wiley, 2022. https://doi.org/10.1002/cne.25229."},"volume":530,"issue":"2","language":[{"iso":"eng"}],"publication_status":"published","publication_identifier":{"eissn":["1096-9861"],"issn":["0021-9967"]},"intvolume":" 530","month":"02","scopus_import":"1","pmid":1,"oa_version":"None","abstract":[{"text":"Neurons can change their classical neurotransmitters during ontogeny, sometimes going through stages of dual release. Here, we explored the development of the neurotransmitter identity of neurons of the avian nucleus isthmi parvocellularis (Ipc), whose axon terminals are retinotopically arranged in the optic tectum (TeO) and exert a focal gating effect upon the ascending transmission of retinal inputs. Although cholinergic and glutamatergic markers are both found in Ipc neurons and terminals of adult pigeons and chicks, the mRNA expression of the vesicular acetylcholine transporter, VAChT, is weak or absent. To explore how the Ipc neurotransmitter identity is established during ontogeny, we analyzed the expression of mRNAs coding for cholinergic (ChAT, VAChT, and CHT) and glutamatergic (VGluT2 and VGluT3) markers in chick embryos at different developmental stages. We found that between E12 and E18, Ipc neurons expressed all cholinergic mRNAs and also VGluT2 mRNA; however, from E16 through posthatch stages, VAChT mRNA expression was specifically diminished. Our ex vivo deposits of tracer crystals and intracellular filling experiments revealed that Ipc axons exhibit a mature paintbrush morphology late in development, experiencing marked morphological transformations during the period of presumptive dual vesicular transmitter release. Additionally, although ChAT protein immunoassays increasingly label the growing Ipc axon, this labeling was consistently restricted to sparse portions of the terminal branches. Combined, these results suggest that the synthesis of glutamate and acetylcholine, and their vesicular release, is complexly linked to the developmental processes of branching, growing and remodeling of these unique axons.","lang":"eng"}],"department":[{"_id":"MaJö"}],"date_updated":"2023-08-11T10:58:17Z","status":"public","type":"journal_article","article_type":"original","_id":"9955"},{"oa_version":"Published Version","abstract":[{"lang":"eng","text":"Novelty facilitates formation of memories. The detection of novelty and storage of contextual memories are both mediated by the hippocampus, yet the mechanisms that link these two functions remain to be defined. Dentate granule cells (GCs) of the dorsal hippocampus fire upon novelty exposure forming engrams of contextual memory. However, their key excitatory inputs from the entorhinal cortex are not responsive to novelty and are insufficient to make dorsal GCs fire reliably. Here we uncover a powerful glutamatergic pathway to dorsal GCs from ventral hippocampal mossy cells (MCs) that relays novelty, and is necessary and sufficient for driving dorsal GCs activation. Furthermore, manipulation of ventral MCs activity bidirectionally regulates novelty-induced contextual memory acquisition. Our results show that ventral MCs activity controls memory formation through an intra-hippocampal interaction mechanism gated by novelty."}],"month":"01","intvolume":" 31","file":[{"content_type":"application/pdf","access_level":"open_access","relation":"main_file","checksum":"b7b9c8bc84a08befce365c675229a7d1","file_id":"8678","success":1,"date_updated":"2020-10-19T13:31:28Z","file_size":4915964,"creator":"dernst","date_created":"2020-10-19T13:31:28Z","file_name":"2021_CurrentBiology_Fredes.pdf"}],"language":[{"iso":"eng"}],"publication_status":"published","related_material":{"link":[{"description":"News on IST Homepage","url":"https://ist.ac.at/en/news/remembering-novelty/","relation":"press_release"}]},"volume":31,"issue":"1","license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","ec_funded":1,"_id":"7551","status":"public","type":"journal_article","article_type":"original","tmp":{"short":"CC BY-NC-ND (4.0)","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png"},"ddc":["570"],"date_updated":"2023-08-04T10:47:11Z","file_date_updated":"2020-10-19T13:31:28Z","department":[{"_id":"MaJö"},{"_id":"RySh"}],"acknowledgement":"We thank Peter Jonas and Peter Somogyi for critically reading the manuscript, Satoshi Kida for helpful discussion, Taijia Makinen for providing the Prox1-creERT2 mouse line, and Hiromu Yawo for the VAMP2-Venus construct. We also thank Vivek Jayaraman, Ph.D.; Rex A. Kerr, Ph.D.; Douglas S. Kim, Ph.D.; Loren L. Looger, Ph.D.; and Karel Svoboda, Ph.D. from the GENIE Project, Janelia Farm Research Campus, Howard Hughes Medical Institute for the viral constructs used for GCaMP6s expression. We also thank Jacqueline Montanaro, Vanessa Zheden, David Kleindienst, and Laura Burnett for technical assistance, as well as Robert Beattie for imaging assistance. This work was supported by a European Research Council Advanced Grant 694539 to R.S.","publisher":"Elsevier","quality_controlled":"1","oa":1,"day":"11","publication":"Current Biology","has_accepted_license":"1","isi":1,"year":"2021","date_published":"2021-01-11T00:00:00Z","doi":"10.1016/j.cub.2020.09.074","date_created":"2020-02-28T10:56:18Z","page":"P25-38.E5","project":[{"call_identifier":"H2020","_id":"25CA28EA-B435-11E9-9278-68D0E5697425","name":"In situ analysis of single channel subunit composition in neurons: physiological implication in synaptic plasticity and behaviour","grant_number":"694539"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"short":"F.A. Fredes Tolorza, M.A. Silva Sifuentes, P. Koppensteiner, K. Kobayashi, M.A. Jösch, R. Shigemoto, Current Biology 31 (2021) P25–38.E5.","ieee":"F. A. Fredes Tolorza, M. A. Silva Sifuentes, P. Koppensteiner, K. Kobayashi, M. A. Jösch, and R. Shigemoto, “Ventro-dorsal hippocampal pathway gates novelty-induced contextual memory formation,” Current Biology, vol. 31, no. 1. Elsevier, p. P25–38.E5, 2021.","ama":"Fredes Tolorza FA, Silva Sifuentes MA, Koppensteiner P, Kobayashi K, Jösch MA, Shigemoto R. Ventro-dorsal hippocampal pathway gates novelty-induced contextual memory formation. Current Biology. 2021;31(1):P25-38.E5. doi:10.1016/j.cub.2020.09.074","apa":"Fredes Tolorza, F. A., Silva Sifuentes, M. A., Koppensteiner, P., Kobayashi, K., Jösch, M. A., & Shigemoto, R. (2021). Ventro-dorsal hippocampal pathway gates novelty-induced contextual memory formation. Current Biology. Elsevier. https://doi.org/10.1016/j.cub.2020.09.074","mla":"Fredes Tolorza, Felipe A., et al. “Ventro-Dorsal Hippocampal Pathway Gates Novelty-Induced Contextual Memory Formation.” Current Biology, vol. 31, no. 1, Elsevier, 2021, p. P25–38.E5, doi:10.1016/j.cub.2020.09.074.","ista":"Fredes Tolorza FA, Silva Sifuentes MA, Koppensteiner P, Kobayashi K, Jösch MA, Shigemoto R. 2021. Ventro-dorsal hippocampal pathway gates novelty-induced contextual memory formation. Current Biology. 31(1), P25–38.E5.","chicago":"Fredes Tolorza, Felipe A, Maria A Silva Sifuentes, Peter Koppensteiner, Kenta Kobayashi, Maximilian A Jösch, and Ryuichi Shigemoto. “Ventro-Dorsal Hippocampal Pathway Gates Novelty-Induced Contextual Memory Formation.” Current Biology. Elsevier, 2021. https://doi.org/10.1016/j.cub.2020.09.074."},"title":"Ventro-dorsal hippocampal pathway gates novelty-induced contextual memory formation","author":[{"full_name":"Fredes Tolorza, Felipe A","last_name":"Fredes Tolorza","id":"384825DA-F248-11E8-B48F-1D18A9856A87","first_name":"Felipe A"},{"first_name":"Maria A","id":"371B3D6E-F248-11E8-B48F-1D18A9856A87","last_name":"Silva Sifuentes","full_name":"Silva Sifuentes, Maria A"},{"last_name":"Koppensteiner","full_name":"Koppensteiner, Peter","first_name":"Peter","id":"3B8B25A8-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Kobayashi","full_name":"Kobayashi, Kenta","first_name":"Kenta"},{"last_name":"Jösch","full_name":"Jösch, Maximilian A","orcid":"0000-0002-3937-1330","id":"2BD278E6-F248-11E8-B48F-1D18A9856A87","first_name":"Maximilian A"},{"first_name":"Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8761-9444","full_name":"Shigemoto, Ryuichi","last_name":"Shigemoto"}],"external_id":{"isi":["000614361000020"]},"article_processing_charge":"No"}]