---
_id: '9887'
abstract:
- lang: eng
text: Clathrin-mediated endocytosis is the major route of entry of cargos into cells
and thus underpins many physiological processes. During endocytosis, an area of
flat membrane is remodeled by proteins to create a spherical vesicle against intracellular
forces. The protein machinery which mediates this membrane bending in plants is
unknown. However, it is known that plant endocytosis is actin independent, thus
indicating that plants utilize a unique mechanism to mediate membrane bending
against high-turgor pressure compared to other model systems. Here, we investigate
the TPLATE complex, a plant-specific endocytosis protein complex. It has been
thought to function as a classical adaptor functioning underneath the clathrin
coat. However, by using biochemical and advanced live microscopy approaches, we
found that TPLATE is peripherally associated with clathrin-coated vesicles and
localizes at the rim of endocytosis events. As this localization is more fitting
to the protein machinery involved in membrane bending during endocytosis, we examined
cells in which the TPLATE complex was disrupted and found that the clathrin structures
present as flat patches. This suggests a requirement of the TPLATE complex for
membrane bending during plant clathrin–mediated endocytosis. Next, we used in
vitro biophysical assays to confirm that the TPLATE complex possesses protein
domains with intrinsic membrane remodeling activity. These results redefine the
role of the TPLATE complex and implicate it as a key component of the evolutionarily
distinct plant endocytosis mechanism, which mediates endocytic membrane bending
against the high-turgor pressure in plant cells.
acknowledged_ssus:
- _id: EM-Fac
- _id: LifeSc
- _id: Bio
acknowledgement: 'We gratefully thank Julie Neveu and Dr. Amanda Barranco of the Grégory
Vert laboratory for help preparing plants in France, Dr. Zuzana Gelova for help
and advice with protoplast generation, Dr. Stéphane Vassilopoulos and Dr. Florian
Schur for advice regarding EM tomography, Alejandro Marquiegui Alvaro for help with
material generation, and Dr. Lukasz Kowalski for generously gifting us the mWasabi
protein. This research was supported by the Scientific Service Units of Institute
of Science and Technology Austria (IST Austria) through resources provided by the
Electron Microscopy Facility, Lab Support Facility (particularly Dorota Jaworska),
and the Bioimaging Facility. We acknowledge the Advanced Microscopy Facility of
the Vienna BioCenter Core Facilities for use of the 3D SIM. For the mass spectrometry
analysis of proteins, we acknowledge the University of Natural Resources and Life
Sciences (BOKU) Core Facility Mass Spectrometry. This work was supported by the
following funds: A.J. is supported by funding from the Austrian Science Fund I3630B25
to J.F. P.M. and E.B. are supported by Agence Nationale de la Recherche ANR-11-EQPX-0029
Morphoscope2 and ANR-10-INBS-04 France BioImaging. S.Y.B. is supported by the NSF
No. 1121998 and 1614915. J.W. and D.V.D. are supported by the European Research
Council Grant 682436 (to D.V.D.), a China Scholarship Council Grant 201508440249
(to J.W.), and by a Ghent University Special Research Co-funding Grant ST01511051
(to J.W.).'
article_number: e2113046118
article_processing_charge: No
article_type: original
author:
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Dana A
full_name: Dahhan, Dana A
last_name: Dahhan
- first_name: Nataliia
full_name: Gnyliukh, Nataliia
id: 390C1120-F248-11E8-B48F-1D18A9856A87
last_name: Gnyliukh
orcid: 0000-0002-2198-0509
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Vanessa
full_name: Zheden, Vanessa
id: 39C5A68A-F248-11E8-B48F-1D18A9856A87
last_name: Zheden
orcid: 0000-0002-9438-4783
- first_name: Tommaso
full_name: Costanzo, Tommaso
id: D93824F4-D9BA-11E9-BB12-F207E6697425
last_name: Costanzo
orcid: 0000-0001-9732-3815
- first_name: Pierre
full_name: Mahou, Pierre
last_name: Mahou
- first_name: Mónika
full_name: Hrtyan, Mónika
id: 45A71A74-F248-11E8-B48F-1D18A9856A87
last_name: Hrtyan
- first_name: Jie
full_name: Wang, Jie
last_name: Wang
- first_name: Juan L
full_name: Aguilera Servin, Juan L
id: 2A67C376-F248-11E8-B48F-1D18A9856A87
last_name: Aguilera Servin
orcid: 0000-0002-2862-8372
- first_name: Daniël
full_name: van Damme, Daniël
last_name: van Damme
- first_name: Emmanuel
full_name: Beaurepaire, Emmanuel
last_name: Beaurepaire
- first_name: Martin
full_name: Loose, Martin
id: 462D4284-F248-11E8-B48F-1D18A9856A87
last_name: Loose
orcid: 0000-0001-7309-9724
- first_name: Sebastian Y
full_name: Bednarek, Sebastian Y
last_name: Bednarek
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Johnson AJ, Dahhan DA, Gnyliukh N, et al. The TPLATE complex mediates membrane
bending during plant clathrin-mediated endocytosis. Proceedings of the National
Academy of Sciences. 2021;118(51). doi:10.1073/pnas.2113046118
apa: Johnson, A. J., Dahhan, D. A., Gnyliukh, N., Kaufmann, W., Zheden, V., Costanzo,
T., … Friml, J. (2021). The TPLATE complex mediates membrane bending during plant
clathrin-mediated endocytosis. Proceedings of the National Academy of Sciences.
National Academy of Sciences. https://doi.org/10.1073/pnas.2113046118
chicago: Johnson, Alexander J, Dana A Dahhan, Nataliia Gnyliukh, Walter Kaufmann,
Vanessa Zheden, Tommaso Costanzo, Pierre Mahou, et al. “The TPLATE Complex Mediates
Membrane Bending during Plant Clathrin-Mediated Endocytosis.” Proceedings of
the National Academy of Sciences. National Academy of Sciences, 2021. https://doi.org/10.1073/pnas.2113046118.
ieee: A. J. Johnson et al., “The TPLATE complex mediates membrane bending
during plant clathrin-mediated endocytosis,” Proceedings of the National Academy
of Sciences, vol. 118, no. 51. National Academy of Sciences, 2021.
ista: Johnson AJ, Dahhan DA, Gnyliukh N, Kaufmann W, Zheden V, Costanzo T, Mahou
P, Hrtyan M, Wang J, Aguilera Servin JL, van Damme D, Beaurepaire E, Loose M,
Bednarek SY, Friml J. 2021. The TPLATE complex mediates membrane bending during
plant clathrin-mediated endocytosis. Proceedings of the National Academy of Sciences.
118(51), e2113046118.
mla: Johnson, Alexander J., et al. “The TPLATE Complex Mediates Membrane Bending
during Plant Clathrin-Mediated Endocytosis.” Proceedings of the National Academy
of Sciences, vol. 118, no. 51, e2113046118, National Academy of Sciences,
2021, doi:10.1073/pnas.2113046118.
short: A.J. Johnson, D.A. Dahhan, N. Gnyliukh, W. Kaufmann, V. Zheden, T. Costanzo,
P. Mahou, M. Hrtyan, J. Wang, J.L. Aguilera Servin, D. van Damme, E. Beaurepaire,
M. Loose, S.Y. Bednarek, J. Friml, Proceedings of the National Academy of Sciences
118 (2021).
date_created: 2021-08-11T14:11:43Z
date_published: 2021-12-14T00:00:00Z
date_updated: 2024-02-19T11:06:09Z
day: '14'
ddc:
- '580'
department:
- _id: JiFr
- _id: MaLo
- _id: EvBe
- _id: EM-Fac
- _id: NanoFab
doi: 10.1073/pnas.2113046118
external_id:
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pmid:
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file:
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file_name: 2021_PNAS_Johnson.pdf
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issue: '51'
language:
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license: https://creativecommons.org/licenses/by/4.0/
month: '12'
oa: 1
oa_version: Published Version
pmid: 1
project:
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call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: Proceedings of the National Academy of Sciences
publication_identifier:
eissn:
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publication_status: published
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quality_controlled: '1'
related_material:
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url: https://doi.org/10.1101/2021.04.26.441441
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status: public
status: public
title: The TPLATE complex mediates membrane bending during plant clathrin-mediated
endocytosis
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image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
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type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 118
year: '2021'
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_id: '14988'
abstract:
- lang: eng
text: Raw data generated from the publication - The TPLATE complex mediates membrane
bending during plant clathrin-mediated endocytosis by Johnson et al., 2021 In
PNAS
article_processing_charge: No
author:
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
citation:
ama: Johnson AJ. Raw data from Johnson et al, PNAS, 2021. 2021. doi:10.5281/ZENODO.5747100
apa: Johnson, A. J. (2021). Raw data from Johnson et al, PNAS, 2021. Zenodo. https://doi.org/10.5281/ZENODO.5747100
chicago: Johnson, Alexander J. “Raw Data from Johnson et Al, PNAS, 2021.” Zenodo,
2021. https://doi.org/10.5281/ZENODO.5747100.
ieee: A. J. Johnson, “Raw data from Johnson et al, PNAS, 2021.” Zenodo, 2021.
ista: Johnson AJ. 2021. Raw data from Johnson et al, PNAS, 2021, Zenodo, 10.5281/ZENODO.5747100.
mla: Johnson, Alexander J. Raw Data from Johnson et Al, PNAS, 2021. Zenodo,
2021, doi:10.5281/ZENODO.5747100.
short: A.J. Johnson, (2021).
date_created: 2024-02-14T14:13:48Z
date_published: 2021-12-01T00:00:00Z
date_updated: 2024-02-19T11:06:09Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.5281/ZENODO.5747100
has_accepted_license: '1'
main_file_link:
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url: https://doi.org/10.5281/zenodo.5747100
month: '12'
oa: 1
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publisher: Zenodo
related_material:
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relation: used_in_publication
status: public
status: public
title: Raw data from Johnson et al, PNAS, 2021
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: research_data_reference
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2021'
...
---
_id: '9992'
abstract:
- lang: eng
text: "Blood – this is what animals use to heal wounds fast and efficient. Plants
do not have blood circulation and their cells cannot move. However, plants have
evolved remarkable capacities to regenerate tissues and organs preventing further
damage. In my PhD research, I studied the wound healing in the Arabidopsis root.
I used a UV laser to ablate single cells in the root tip and observed the consequent
wound healing. Interestingly, the inner adjacent cells induced a\r\ndivision plane
switch and subsequently adopted the cell type of the killed cell to replace it.
We termed this form of wound healing “restorative divisions”. This initial observation
triggered the questions of my PhD studies: How and why do cells orient their division
planes, how do they feel the wound and why does this happen only in inner adjacent
cells.\r\nFor answering these questions, I used a quite simple experimental setup:
5 day - old seedlings were stained with propidium iodide to visualize cell walls
and dead cells; ablation was carried out using a special laser cutter and a confocal
microscope. Adaptation of the novel vertical microscope system made it possible
to observe wounds in real time. This revealed that restorative divisions occur
at increased frequency compared to normal divisions. Additionally,\r\nthe major
plant hormone auxin accumulates in wound adjacent cells and drives the expression
of the wound-stress responsive transcription factor ERF115. Using this as a marker
gene for wound responses, we found that an important part of wound signalling
is the sensing of the collapse of the ablated cell. The collapse causes a radical
pressure drop, which results in strong tissue deformations. These deformations
manifest in an invasion of the now free spot specifically by the inner adjacent
cells within seconds, probably because of higher pressure of the inner tissues.
Long-term imaging revealed that those deformed cells continuously expand towards
the wound hole and that this is crucial for the restorative division. These wound-expanding
cells exhibit an abnormal, biphasic polarity of microtubule arrays\r\nbefore the
division. Experiments inhibiting cell expansion suggest that it is the biphasic
stretching that induces those MT arrays. Adapting the micromanipulator aspiration
system from animal scientists at our institute confirmed the hypothesis that stretching
influences microtubule stability. In conclusion, this shows that microtubules
react to tissue deformation\r\nand this facilitates the observed division plane
switch. This puts mechanical cues and tensions at the most prominent position
for explaining the growth and wound healing properties of plants. Hence, it shines
light onto the importance of understanding mechanical signal transduction. "
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
orcid: 0000-0001-8295-2926
citation:
ama: Hörmayer L. Wound healing in the Arabidopsis root meristem. 2021. doi:10.15479/at:ista:9992
apa: Hörmayer, L. (2021). Wound healing in the Arabidopsis root meristem.
Institute of Science and Technology Austria. https://doi.org/10.15479/at:ista:9992
chicago: Hörmayer, Lukas. “Wound Healing in the Arabidopsis Root Meristem.” Institute
of Science and Technology Austria, 2021. https://doi.org/10.15479/at:ista:9992.
ieee: L. Hörmayer, “Wound healing in the Arabidopsis root meristem,” Institute of
Science and Technology Austria, 2021.
ista: Hörmayer L. 2021. Wound healing in the Arabidopsis root meristem. Institute
of Science and Technology Austria.
mla: Hörmayer, Lukas. Wound Healing in the Arabidopsis Root Meristem. Institute
of Science and Technology Austria, 2021, doi:10.15479/at:ista:9992.
short: L. Hörmayer, Wound Healing in the Arabidopsis Root Meristem, Institute of
Science and Technology Austria, 2021.
date_created: 2021-09-09T07:37:20Z
date_published: 2021-09-13T00:00:00Z
date_updated: 2023-09-07T13:38:33Z
day: '13'
ddc:
- '575'
degree_awarded: PhD
department:
- _id: GradSch
- _id: JiFr
doi: 10.15479/at:ista:9992
ec_funded: 1
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creator: lhoermaye
date_created: 2021-09-09T07:29:48Z
date_updated: 2021-09-15T22:30:26Z
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file_id: '9993'
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date_created: 2021-09-09T14:25:08Z
date_updated: 2021-09-15T22:30:26Z
embargo: 2021-09-09
file_id: '9996'
file_name: Thesis_vfinal_pdfa.pdf
file_size: 6246900
relation: main_file
file_date_updated: 2021-09-15T22:30:26Z
has_accepted_license: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '09'
oa: 1
oa_version: Published Version
page: '168'
project:
- _id: 262EF96E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29988
name: RNA-directed DNA methylation in plant development
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '6351'
relation: part_of_dissertation
status: public
- id: '6943'
relation: part_of_dissertation
status: public
- id: '8002'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
title: Wound healing in the Arabidopsis root meristem
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2021'
...
---
_id: '9010'
abstract:
- lang: eng
text: Availability of the essential macronutrient nitrogen in soil plays a critical
role in plant growth, development, and impacts agricultural productivity. Plants
have evolved different strategies for sensing and responding to heterogeneous
nitrogen distribution. Modulation of root system architecture, including primary
root growth and branching, is among the most essential plant adaptions to ensure
adequate nitrogen acquisition. However, the immediate molecular pathways coordinating
the adjustment of root growth in response to distinct nitrogen sources, such as
nitrate or ammonium, are poorly understood. Here, we show that growth as manifested
by cell division and elongation is synchronized by coordinated auxin flux between
two adjacent outer tissue layers of the root. This coordination is achieved by
nitrate‐dependent dephosphorylation of the PIN2 auxin efflux carrier at a previously
uncharacterized phosphorylation site, leading to subsequent PIN2 lateralization
and thereby regulating auxin flow between adjacent tissues. A dynamic computer
model based on our experimental data successfully recapitulates experimental observations.
Our study provides mechanistic insights broadening our understanding of root growth
mechanisms in dynamic environments.
acknowledged_ssus:
- _id: Bio
acknowledgement: 'We acknowledge Gergely Molnar for critical reading of the manuscript,
Alexander Johnson for language editing and Yulija Salanenka for technical assistance.
Work in the Benkova laboratory was supported by the Austrian Science Fund (FWF01_I1774S)
to KO, RA and EB. Work in the Benkova laboratory was supported by the Austrian Science
Fund (FWF01_I1774S) to KO, RA and EB and by the DOC Fellowship Programme of the
AustrianAcademy of Sciences (25008) to C.A. Work in the Wabnik laboratory was supported
by the Programa de Atraccion de Talento 2017 (Comunidad deMadrid, 2017-T1/BIO-5654
to K.W.), Severo Ochoa Programme for Centres of Excellence in R&D from the Agencia
Estatal de Investigacion of Spain (grantSEV-2016-0672 (2017-2021) to K.W. via the
CBGP) and Programa Estatal de Generacion del Conocimiento y Fortalecimiento Científico
y Tecnologico del Sistema de I+D+I 2019 (PGC2018-093387-A-I00) from MICIU (to K.W.).
M.M.was supported by a postdoctoral contract associated to SEV-2016-0672.We acknowledge
the Bioimaging Facility in IST-Austria and the Advanced Microscopy Facility of the
Vienna Bio Center Core Facilities, member of the Vienna Bio Center Austria, for
use of the OMX v43D SIM microscope. AJ was supported by the Austrian Science Fund
(FWF): I03630 to J.F'
article_number: e106862
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Krisztina
full_name: Ötvös, Krisztina
id: 29B901B0-F248-11E8-B48F-1D18A9856A87
last_name: Ötvös
orcid: 0000-0002-5503-4983
- first_name: Marco
full_name: Marconi, Marco
last_name: Marconi
- first_name: Andrea
full_name: Vega, Andrea
last_name: Vega
- first_name: Jose
full_name: O’Brien, Jose
last_name: O’Brien
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Rashed
full_name: Abualia, Rashed
id: 4827E134-F248-11E8-B48F-1D18A9856A87
last_name: Abualia
orcid: 0000-0002-9357-9415
- first_name: Livio
full_name: Antonielli, Livio
last_name: Antonielli
- first_name: Juan C
full_name: Montesinos López, Juan C
id: 310A8E3E-F248-11E8-B48F-1D18A9856A87
last_name: Montesinos López
orcid: 0000-0001-9179-6099
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Candela
full_name: Cuesta, Candela
id: 33A3C818-F248-11E8-B48F-1D18A9856A87
last_name: Cuesta
orcid: 0000-0003-1923-2410
- first_name: Christina
full_name: Artner, Christina
id: 45DF286A-F248-11E8-B48F-1D18A9856A87
last_name: Artner
- first_name: Eleonore
full_name: Bouguyon, Eleonore
last_name: Bouguyon
- first_name: Alain
full_name: Gojon, Alain
last_name: Gojon
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Rodrigo A.
full_name: Gutiérrez, Rodrigo A.
last_name: Gutiérrez
- first_name: Krzysztof T
full_name: Wabnik, Krzysztof T
id: 4DE369A4-F248-11E8-B48F-1D18A9856A87
last_name: Wabnik
orcid: 0000-0001-7263-0560
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Ötvös K, Marconi M, Vega A, et al. Modulation of plant root growth by nitrogen
source-defined regulation of polar auxin transport. EMBO Journal. 2021;40(3).
doi:10.15252/embj.2020106862
apa: Ötvös, K., Marconi, M., Vega, A., O’Brien, J., Johnson, A. J., Abualia, R.,
… Benková, E. (2021). Modulation of plant root growth by nitrogen source-defined
regulation of polar auxin transport. EMBO Journal. Embo Press. https://doi.org/10.15252/embj.2020106862
chicago: Ötvös, Krisztina, Marco Marconi, Andrea Vega, Jose O’Brien, Alexander J
Johnson, Rashed Abualia, Livio Antonielli, et al. “Modulation of Plant Root Growth
by Nitrogen Source-Defined Regulation of Polar Auxin Transport.” EMBO Journal.
Embo Press, 2021. https://doi.org/10.15252/embj.2020106862.
ieee: K. Ötvös et al., “Modulation of plant root growth by nitrogen source-defined
regulation of polar auxin transport,” EMBO Journal, vol. 40, no. 3. Embo
Press, 2021.
ista: Ötvös K, Marconi M, Vega A, O’Brien J, Johnson AJ, Abualia R, Antonielli L,
Montesinos López JC, Zhang Y, Tan S, Cuesta C, Artner C, Bouguyon E, Gojon A,
Friml J, Gutiérrez RA, Wabnik KT, Benková E. 2021. Modulation of plant root growth
by nitrogen source-defined regulation of polar auxin transport. EMBO Journal.
40(3), e106862.
mla: Ötvös, Krisztina, et al. “Modulation of Plant Root Growth by Nitrogen Source-Defined
Regulation of Polar Auxin Transport.” EMBO Journal, vol. 40, no. 3, e106862,
Embo Press, 2021, doi:10.15252/embj.2020106862.
short: K. Ötvös, M. Marconi, A. Vega, J. O’Brien, A.J. Johnson, R. Abualia, L. Antonielli,
J.C. Montesinos López, Y. Zhang, S. Tan, C. Cuesta, C. Artner, E. Bouguyon, A.
Gojon, J. Friml, R.A. Gutiérrez, K.T. Wabnik, E. Benková, EMBO Journal 40 (2021).
date_created: 2021-01-17T23:01:12Z
date_published: 2021-02-01T00:00:00Z
date_updated: 2024-03-27T23:30:39Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
- _id: EvBe
doi: 10.15252/embj.2020106862
external_id:
isi:
- '000604645600001'
pmid:
- ' 33399250'
file:
- access_level: open_access
checksum: dc55c900f3b061d6c2790b8813d759a3
content_type: application/pdf
creator: dernst
date_created: 2021-02-11T12:28:29Z
date_updated: 2021-02-11T12:28:29Z
file_id: '9110'
file_name: 2021_Embo_Otvos.pdf
file_size: 2358617
relation: main_file
success: 1
file_date_updated: 2021-02-11T12:28:29Z
has_accepted_license: '1'
intvolume: ' 40'
isi: 1
issue: '3'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 2542D156-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I 1774-B16
name: Hormone cross-talk drives nutrient dependent plant development
- _id: 2685A872-B435-11E9-9278-68D0E5697425
name: Hormonal regulation of plant adaptive responses to environmental signals
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: EMBO Journal
publication_identifier:
eissn:
- '14602075'
issn:
- '02614189'
publication_status: published
publisher: Embo Press
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/a-plants-way-to-its-favorite-food/
record:
- id: '10303'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Modulation of plant root growth by nitrogen source-defined regulation of polar
auxin transport
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 40
year: '2021'
...
---
_id: '8931'
abstract:
- lang: eng
text: "Auxin is a major plant growth regulator, but current models on auxin perception
and signaling cannot explain the whole plethora of auxin effects, in particular
those associated with rapid responses. A possible candidate for a component of
additional auxin perception mechanisms is the AUXIN BINDING PROTEIN 1 (ABP1),
whose function in planta remains unclear.\r\nHere we combined expression analysis
with gain- and loss-of-function approaches to analyze the role of ABP1 in plant
development. ABP1 shows a broad expression largely overlapping with, but not regulated
by, transcriptional auxin response activity. Furthermore, ABP1 activity is not
essential for the transcriptional auxin signaling. Genetic in planta analysis
revealed that abp1 loss-of-function mutants show largely normal development with
minor defects in bolting. On the other hand, ABP1 gain-of-function alleles show
a broad range of growth and developmental defects, including root and hypocotyl
growth and bending, lateral root and leaf development, bolting, as well as response
to heat stress. At the cellular level, ABP1 gain-of-function leads to impaired
auxin effect on PIN polar distribution and affects BFA-sensitive PIN intracellular
aggregation.\r\nThe gain-of-function analysis suggests a broad, but still mechanistically
unclear involvement of ABP1 in plant development, possibly masked in abp1 loss-of-function
mutants by a functional redundancy."
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: We would like to acknowledge Bioimaging and Life Science Facilities
at IST Austria for continuous support and also the Plant Sciences Core Facility
of CEITEC Masaryk University for their support with obtaining a part of the scientific
data. We gratefully acknowledge Lindy Abas for help with ABP1::GFP-ABP1 construct
design. This project has received funding from the European Research Council (ERC)
under the European Union’s Horizon 2020 research and innovation program [grant agreement
no. 742985] and Austrian Science Fund (FWF) [I 3630-B25] to J.F.; DOC Fellowship
of the Austrian Academy of Sciences to L.L.; the European Structural and Investment
Funds, Operational Programme Research, Development and Education - Project „MSCAfellow@MUNI“
[CZ.02.2.69/0.0/0.0/17_050/0008496] to M.P.. This project was also supported by
the Czech Science Foundation [GA 20-20860Y] to M.Z and MEYS CR [project no.CZ.02.1.01/0.0/0.0/16_019/0000738]
to M. Č.
article_number: '110750'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Zuzana
full_name: Gelová, Zuzana
id: 0AE74790-0E0B-11E9-ABC7-1ACFE5697425
last_name: Gelová
orcid: 0000-0003-4783-1752
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Markéta
full_name: Pernisová, Markéta
last_name: Pernisová
- first_name: Géraldine
full_name: Brunoud, Géraldine
last_name: Brunoud
- first_name: Xixi
full_name: Zhang, Xixi
id: 61A66458-47E9-11EA-85BA-8AEAAF14E49A
last_name: Zhang
orcid: 0000-0001-7048-4627
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Jaroslav
full_name: Michalko, Jaroslav
id: 483727CA-F248-11E8-B48F-1D18A9856A87
last_name: Michalko
- first_name: Zlata
full_name: Pavlovicova, Zlata
last_name: Pavlovicova
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
- first_name: Jakub
full_name: Hajny, Jakub
id: 4800CC20-F248-11E8-B48F-1D18A9856A87
last_name: Hajny
orcid: 0000-0003-2140-7195
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Milada
full_name: Čovanová, Milada
last_name: Čovanová
- first_name: Marta
full_name: Zwiewka, Marta
last_name: Zwiewka
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
orcid: 0000-0001-8295-2926
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: Tongda
full_name: Xu, Tongda
last_name: Xu
- first_name: Teva
full_name: Vernoux, Teva
last_name: Vernoux
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Gelová Z, Gallei MC, Pernisová M, et al. Developmental roles of auxin binding
protein 1 in Arabidopsis thaliana. Plant Science. 2021;303. doi:10.1016/j.plantsci.2020.110750
apa: Gelová, Z., Gallei, M. C., Pernisová, M., Brunoud, G., Zhang, X., Glanc, M.,
… Friml, J. (2021). Developmental roles of auxin binding protein 1 in Arabidopsis
thaliana. Plant Science. Elsevier. https://doi.org/10.1016/j.plantsci.2020.110750
chicago: Gelová, Zuzana, Michelle C Gallei, Markéta Pernisová, Géraldine Brunoud,
Xixi Zhang, Matous Glanc, Lanxin Li, et al. “Developmental Roles of Auxin Binding
Protein 1 in Arabidopsis Thaliana.” Plant Science. Elsevier, 2021. https://doi.org/10.1016/j.plantsci.2020.110750.
ieee: Z. Gelová et al., “Developmental roles of auxin binding protein 1 in
Arabidopsis thaliana,” Plant Science, vol. 303. Elsevier, 2021.
ista: Gelová Z, Gallei MC, Pernisová M, Brunoud G, Zhang X, Glanc M, Li L, Michalko
J, Pavlovicova Z, Verstraeten I, Han H, Hajny J, Hauschild R, Čovanová M, Zwiewka
M, Hörmayer L, Fendrych M, Xu T, Vernoux T, Friml J. 2021. Developmental roles
of auxin binding protein 1 in Arabidopsis thaliana. Plant Science. 303, 110750.
mla: Gelová, Zuzana, et al. “Developmental Roles of Auxin Binding Protein 1 in Arabidopsis
Thaliana.” Plant Science, vol. 303, 110750, Elsevier, 2021, doi:10.1016/j.plantsci.2020.110750.
short: Z. Gelová, M.C. Gallei, M. Pernisová, G. Brunoud, X. Zhang, M. Glanc, L.
Li, J. Michalko, Z. Pavlovicova, I. Verstraeten, H. Han, J. Hajny, R. Hauschild,
M. Čovanová, M. Zwiewka, L. Hörmayer, M. Fendrych, T. Xu, T. Vernoux, J. Friml,
Plant Science 303 (2021).
date_created: 2020-12-09T14:48:28Z
date_published: 2021-02-01T00:00:00Z
date_updated: 2024-03-27T23:30:43Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
- _id: Bio
doi: 10.1016/j.plantsci.2020.110750
ec_funded: 1
external_id:
isi:
- '000614154500001'
pmid:
- '33487339'
file:
- access_level: open_access
checksum: a7f2562bdca62d67dfa88e271b62a629
content_type: application/pdf
creator: dernst
date_created: 2021-02-04T07:49:25Z
date_updated: 2021-02-04T07:49:25Z
file_id: '9083'
file_name: 2021_PlantScience_Gelova.pdf
file_size: 12563728
relation: main_file
success: 1
file_date_updated: 2021-02-04T07:49:25Z
has_accepted_license: '1'
intvolume: ' 303'
isi: 1
keyword:
- Agronomy and Crop Science
- Plant Science
- Genetics
- General Medicine
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 26B4D67E-B435-11E9-9278-68D0E5697425
grant_number: '25351'
name: 'A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated
Rapid Growth Inhibition in Arabidopsis Root'
publication: Plant Science
publication_identifier:
issn:
- 0168-9452
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
record:
- id: '11626'
relation: dissertation_contains
status: public
- id: '10083'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Developmental roles of auxin binding protein 1 in Arabidopsis thaliana
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 303
year: '2021'
...
---
_id: '9287'
abstract:
- lang: eng
text: "The phytohormone auxin and its directional transport through tissues are
intensively studied. However, a mechanistic understanding of auxin-mediated feedback
on endocytosis and polar distribution of PIN auxin transporters remains limited
due to contradictory observations and interpretations. Here, we used state-of-the-art
methods to reexamine the\r\nauxin effects on PIN endocytic trafficking. We used
high auxin concentrations or longer treatments versus lower concentrations and
shorter treatments of natural (IAA) and synthetic (NAA) auxins to distinguish
between specific and nonspecific effects. Longer treatments of both auxins interfere
with Brefeldin A-mediated intracellular PIN2 accumulation and also with general
aggregation of endomembrane compartments. NAA treatment decreased the internalization
of the endocytic tracer dye, FM4-64; however, NAA treatment also affected the
number, distribution, and compartment identity of the early endosome/trans-Golgi
network (EE/TGN), rendering the FM4-64 endocytic assays at high NAA concentrations
unreliable. To circumvent these nonspecific effects of NAA and IAA affecting the
endomembrane system, we opted for alternative approaches visualizing the endocytic
events directly at the plasma membrane (PM). Using Total Internal Reflection Fluorescence
(TIRF) microscopy, we saw no significant effects of IAA or NAA treatments on the
incidence and dynamics of clathrin foci, implying that these treatments do not
affect the overall endocytosis rate. However, both NAA and IAA at low concentrations
rapidly and specifically promoted endocytosis of photo-converted PIN2 from the
PM. These analyses identify a specific effect of NAA and IAA on PIN2 endocytosis,
thus contributing to its\r\npolarity maintenance and furthermore illustrate that
high auxin levels have nonspecific effects on trafficking and endomembrane compartments. "
acknowledged_ssus:
- _id: M-Shop
- _id: Bio
acknowledgement: 'We thank Ivan Kulik for developing the Chip’n’Dale apparatus with
Lanxin Li; the IST machine shop and the Bioimaging facility for their excellent
support; Matouš Glanc and Matyáš Fendrych for their valuable discussions and help;
Barbara Casillas-Perez for her help with statistics. This project has received funding
from the European Research Council (ERC) under the European Union''s Horizon 2020
research and innovation program (grant agreement No 742985). A.J. is supported by
funding from the Austrian Science Fund (FWF): I3630B25 to J.F. '
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Madhumitha
full_name: Narasimhan, Madhumitha
id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
last_name: Narasimhan
orcid: 0000-0002-8600-0671
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Lesia
full_name: Rodriguez Solovey, Lesia
id: 3922B506-F248-11E8-B48F-1D18A9856A87
last_name: Rodriguez Solovey
orcid: 0000-0002-7244-7237
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
- first_name: E
full_name: Himschoot, E
last_name: Himschoot
- first_name: R
full_name: Wang, R
last_name: Wang
- first_name: S
full_name: Vanneste, S
last_name: Vanneste
- first_name: J
full_name: Sánchez-Simarro, J
last_name: Sánchez-Simarro
- first_name: F
full_name: Aniento, F
last_name: Aniento
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Narasimhan M, Gallei MC, Tan S, et al. Systematic analysis of specific and
nonspecific auxin effects on endocytosis and trafficking. Plant Physiology.
2021;186(2):1122–1142. doi:10.1093/plphys/kiab134
apa: Narasimhan, M., Gallei, M. C., Tan, S., Johnson, A. J., Verstraeten, I., Li,
L., … Friml, J. (2021). Systematic analysis of specific and nonspecific auxin
effects on endocytosis and trafficking. Plant Physiology. Oxford University
Press. https://doi.org/10.1093/plphys/kiab134
chicago: Narasimhan, Madhumitha, Michelle C Gallei, Shutang Tan, Alexander J Johnson,
Inge Verstraeten, Lanxin Li, Lesia Rodriguez Solovey, et al. “Systematic Analysis
of Specific and Nonspecific Auxin Effects on Endocytosis and Trafficking.” Plant
Physiology. Oxford University Press, 2021. https://doi.org/10.1093/plphys/kiab134.
ieee: M. Narasimhan et al., “Systematic analysis of specific and nonspecific
auxin effects on endocytosis and trafficking,” Plant Physiology, vol. 186,
no. 2. Oxford University Press, pp. 1122–1142, 2021.
ista: Narasimhan M, Gallei MC, Tan S, Johnson AJ, Verstraeten I, Li L, Rodriguez
Solovey L, Han H, Himschoot E, Wang R, Vanneste S, Sánchez-Simarro J, Aniento
F, Adamowski M, Friml J. 2021. Systematic analysis of specific and nonspecific
auxin effects on endocytosis and trafficking. Plant Physiology. 186(2), 1122–1142.
mla: Narasimhan, Madhumitha, et al. “Systematic Analysis of Specific and Nonspecific
Auxin Effects on Endocytosis and Trafficking.” Plant Physiology, vol. 186,
no. 2, Oxford University Press, 2021, pp. 1122–1142, doi:10.1093/plphys/kiab134.
short: M. Narasimhan, M.C. Gallei, S. Tan, A.J. Johnson, I. Verstraeten, L. Li,
L. Rodriguez Solovey, H. Han, E. Himschoot, R. Wang, S. Vanneste, J. Sánchez-Simarro,
F. Aniento, M. Adamowski, J. Friml, Plant Physiology 186 (2021) 1122–1142.
date_created: 2021-03-26T12:08:38Z
date_published: 2021-06-01T00:00:00Z
date_updated: 2024-03-27T23:30:43Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1093/plphys/kiab134
ec_funded: 1
external_id:
isi:
- '000671555900031'
pmid:
- '33734402'
file:
- access_level: open_access
checksum: 532bb9469d3b665907f06df8c383eade
content_type: application/pdf
creator: cziletti
date_created: 2021-11-11T15:07:51Z
date_updated: 2021-11-11T15:07:51Z
file_id: '10273'
file_name: 2021_PlantPhysio_Narasimhan.pdf
file_size: 2289127
relation: main_file
success: 1
file_date_updated: 2021-11-11T15:07:51Z
has_accepted_license: '1'
intvolume: ' 186'
isi: 1
issue: '2'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
page: 1122–1142
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: Plant Physiology
publication_identifier:
eissn:
- 1532-2548
issn:
- 0032-0889
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
related_material:
link:
- relation: erratum
url: 10.1093/plphys/kiab380
record:
- id: '11626'
relation: dissertation_contains
status: public
- id: '10083'
relation: dissertation_contains
status: public
status: public
title: Systematic analysis of specific and nonspecific auxin effects on endocytosis
and trafficking
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 186
year: '2021'
...
---
_id: '10083'
abstract:
- lang: eng
text: "Plant motions occur across a wide spectrum of timescales, ranging from seed
dispersal through bursting (milliseconds) and stomatal opening (minutes) to long-term
adaptation of gross architecture. Relatively fast motions include water-driven
growth as exemplified by root cell expansion under abiotic/biotic stresses or
during gravitropism. A showcase is a root growth inhibition in 30 seconds triggered
by the phytohormone auxin. However, the cellular and molecular mechanisms are
still largely unknown. This thesis covers the studies about this topic as follows.
By taking advantage of microfluidics combined with live imaging, pharmaceutical
tools, and transgenic lines, we examined the kinetics of and causal relationship
among various auxininduced rapid cellular changes in root growth, apoplastic pH,
cytosolic Ca2+, cortical microtubule (CMT) orientation, and vacuolar morphology.
We revealed that CMT reorientation and vacuolar constriction are the consequence
of growth itself instead of responding directly to auxin. In contrast, auxin induces
apoplast alkalinization to rapidly inhibit root growth in 30 seconds. This auxin-triggered
apoplast alkalinization results from rapid H+- influx that is contributed by Ca2+
inward channel CYCLIC NUCLEOTIDE-GATED CHANNEL 14 (CNGC14)-dependent Ca2+ signaling.
To dissect which auxin signaling mediates the rapid apoplast alkalinization, we\r\ncombined
microfluidics and genetic engineering to verify that TIR1/AFB receptors conduct
a non-transcriptional regulation on Ca2+ and H+ -influx. This non-canonical pathway
is mostly mediated by the cytosolic portion of TIR1/AFB. On the other hand, we
uncovered, using biochemical and phospho-proteomic analysis, that auxin cell surface
signaling component TRANSMEMBRANE KINASE 1 (TMK1) plays a negative role during
auxin-trigger apoplast\r\nalkalinization and root growth inhibition through directly
activating PM H+ -ATPases. Therefore, we discovered that PM H+ -ATPases counteract
instead of mediate the auxintriggered rapid H+ -influx, and that TIR1/AFB and
TMK1 regulate root growth antagonistically. This opposite effect of TIR1/AFB and
TMK1 is consistent during auxin-induced hypocotyl elongation, leading us to explore
the relation of two signaling pathways. Assisted with biochemistry and fluorescent
imaging, we verified for the first time that TIR1/AFB and TMK1 can interact with
each other. The ability of TIR1/AFB binding to membrane lipid provides a basis
for the interaction of plasma membrane- and cytosol-localized proteins.\r\nBesides,
transgenic analysis combined with genetic engineering and biochemistry showed
that vi\r\nthey do function in the same pathway. Particularly, auxin-induced
TMK1 increase is TIR1/AFB dependent, suggesting TIR1/AFB regulation on TMK1. Conversely,
TMK1 also regulates TIR1/AFB protein levels and thus auxin canonical signaling.
To follow the study of rapid growth regulation, we analyzed another rapid growth
regulator, signaling peptide RALF1. We showed that RALF1 also triggers a rapid
and reversible growth inhibition caused by H + influx, highly resembling but not
dependent on auxin. Besides, RALF1 promotes auxin biosynthesis by increasing expression
of auxin biosynthesis enzyme YUCCAs and thus induces auxin signaling in ca. 1
hour, contributing to the sustained RALF1-triggered growth inhibition. These studies
collectively contribute to understanding rapid regulation on plant cell\r\ngrowth,
novel auxin signaling pathway as well as auxin-peptide crosstalk. "
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Lanxin
full_name: Li, Lanxin
last_name: Li
citation:
ama: Li L. Rapid cell growth regulation in Arabidopsis. 2021. doi:10.15479/at:ista:10083
apa: Li, L. (2021). Rapid cell growth regulation in Arabidopsis. Institute
of Science and Technology Austria. https://doi.org/10.15479/at:ista:10083
chicago: Li, Lanxin. “Rapid Cell Growth Regulation in Arabidopsis.” Institute of
Science and Technology Austria, 2021. https://doi.org/10.15479/at:ista:10083.
ieee: L. Li, “Rapid cell growth regulation in Arabidopsis,” Institute of Science
and Technology Austria, 2021.
ista: Li L. 2021. Rapid cell growth regulation in Arabidopsis. Institute of Science
and Technology Austria.
mla: Li, Lanxin. Rapid Cell Growth Regulation in Arabidopsis. Institute of
Science and Technology Austria, 2021, doi:10.15479/at:ista:10083.
short: L. Li, Rapid Cell Growth Regulation in Arabidopsis, Institute of Science
and Technology Austria, 2021.
date_created: 2021-10-04T13:33:10Z
date_published: 2021-10-06T00:00:00Z
date_updated: 2023-10-31T19:30:02Z
day: '06'
ddc:
- '575'
degree_awarded: PhD
department:
- _id: GradSch
- _id: JiFr
doi: 10.15479/at:ista:10083
ec_funded: 1
file:
- access_level: open_access
checksum: 3b2f55b3b8ae05337a0dcc1cd8595b10
content_type: application/pdf
creator: cchlebak
date_created: 2021-10-14T08:00:07Z
date_updated: 2022-12-20T23:30:03Z
embargo: 2022-10-14
file_id: '10138'
file_name: 0._IST_Austria_Thesis_Lanxin_Li_1014_pdftron.pdf
file_size: 8616142
relation: main_file
- access_level: closed
checksum: f23ed258ca894f6aabf58b0c128bf242
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: cchlebak
date_created: 2021-10-14T08:00:13Z
date_updated: 2022-12-20T23:30:03Z
embargo_to: open_access
file_id: '10139'
file_name: 0._IST_Austria_Thesis_Lanxin_Li_1014.docx
file_size: 15058499
relation: source_file
file_date_updated: 2022-12-20T23:30:03Z
has_accepted_license: '1'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
- _id: 26B4D67E-B435-11E9-9278-68D0E5697425
grant_number: '25351'
name: 'A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated
Rapid Growth Inhibition in Arabidopsis Root'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '442'
relation: part_of_dissertation
status: public
- id: '8931'
relation: part_of_dissertation
status: public
- id: '9287'
relation: part_of_dissertation
status: public
- id: '8283'
relation: part_of_dissertation
status: public
- id: '8986'
relation: part_of_dissertation
status: public
- id: '6627'
relation: part_of_dissertation
status: public
- id: '10095'
relation: part_of_dissertation
status: public
- id: '10015'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
title: Rapid cell growth regulation in Arabidopsis
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2021'
...
---
_id: '10015'
abstract:
- lang: eng
text: "Auxin plays a dual role in growth regulation and, depending on the tissue
and concentration of the hormone, it can either promote or inhibit division and
expansion processes in plants. Recent studies have revealed that, beyond transcriptional
reprogramming, alternative auxincontrolled mechanisms regulate root growth. Here,
we explored the impact of different concentrations of the synthetic auxin NAA
that establish growth-promoting and -repressing conditions on the root tip proteome
and phosphoproteome, generating a unique resource. From the phosphoproteome data,
we pinpointed (novel) growth regulators, such as the RALF34-THE1 module. Our results,
together with previously published studies, suggest that auxin, H+-ATPases, cell
wall modifications and cell wall sensing receptor-like kinases are tightly embedded
in a pathway regulating cell elongation. Furthermore, our study assigned a novel
role to MKK2 as a regulator of primary root growth and a (potential) regulator
of auxin biosynthesis and signalling, and suggests the importance of the MKK2\r\nThr31
phosphorylation site for growth regulation in the Arabidopsis root tip."
acknowledgement: We thank the Nottingham Stock Centre for seeds, Frank Van Breusegem
for the phb3 mutant, and Herman Höfte for the the1 mutant. Open Access Funding by
the Austrian Science Fund (FWF).
alternative_title:
- Protein Phosphorylation and Cell Signaling in Plants
article_number: '1665 '
article_processing_charge: Yes
article_type: original
author:
- first_name: N
full_name: Nikonorova, N
last_name: Nikonorova
- first_name: E
full_name: Murphy, E
last_name: Murphy
- first_name: CF
full_name: Fonseca de Lima, CF
last_name: Fonseca de Lima
- first_name: S
full_name: Zhu, S
last_name: Zhu
- first_name: B
full_name: van de Cotte, B
last_name: van de Cotte
- first_name: LD
full_name: Vu, LD
last_name: Vu
- first_name: D
full_name: Balcerowicz, D
last_name: Balcerowicz
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: X
full_name: Kong, X
last_name: Kong
- first_name: G
full_name: De Rop, G
last_name: De Rop
- first_name: T
full_name: Beeckman, T
last_name: Beeckman
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: K
full_name: Vissenberg, K
last_name: Vissenberg
- first_name: PC
full_name: Morris, PC
last_name: Morris
- first_name: Z
full_name: Ding, Z
last_name: Ding
- first_name: I
full_name: De Smet, I
last_name: De Smet
citation:
ama: Nikonorova N, Murphy E, Fonseca de Lima C, et al. The Arabidopsis root tip
(phospho)proteomes at growth-promoting versus growth-repressing conditions reveal
novel root growth regulators. Cells. 2021;10. doi:10.3390/cells10071665
apa: Nikonorova, N., Murphy, E., Fonseca de Lima, C., Zhu, S., van de Cotte, B.,
Vu, L., … De Smet, I. (2021). The Arabidopsis root tip (phospho)proteomes at growth-promoting
versus growth-repressing conditions reveal novel root growth regulators. Cells.
MDPI. https://doi.org/10.3390/cells10071665
chicago: Nikonorova, N, E Murphy, CF Fonseca de Lima, S Zhu, B van de Cotte, LD
Vu, D Balcerowicz, et al. “The Arabidopsis Root Tip (Phospho)Proteomes at Growth-Promoting
versus Growth-Repressing Conditions Reveal Novel Root Growth Regulators.” Cells.
MDPI, 2021. https://doi.org/10.3390/cells10071665.
ieee: N. Nikonorova et al., “The Arabidopsis root tip (phospho)proteomes
at growth-promoting versus growth-repressing conditions reveal novel root growth
regulators,” Cells, vol. 10. MDPI, 2021.
ista: Nikonorova N, Murphy E, Fonseca de Lima C, Zhu S, van de Cotte B, Vu L, Balcerowicz
D, Li L, Kong X, De Rop G, Beeckman T, Friml J, Vissenberg K, Morris P, Ding Z,
De Smet I. 2021. The Arabidopsis root tip (phospho)proteomes at growth-promoting
versus growth-repressing conditions reveal novel root growth regulators. Cells.
10, 1665.
mla: Nikonorova, N., et al. “The Arabidopsis Root Tip (Phospho)Proteomes at Growth-Promoting
versus Growth-Repressing Conditions Reveal Novel Root Growth Regulators.” Cells,
vol. 10, 1665, MDPI, 2021, doi:10.3390/cells10071665.
short: N. Nikonorova, E. Murphy, C. Fonseca de Lima, S. Zhu, B. van de Cotte, L.
Vu, D. Balcerowicz, L. Li, X. Kong, G. De Rop, T. Beeckman, J. Friml, K. Vissenberg,
P. Morris, Z. Ding, I. De Smet, Cells 10 (2021).
date_created: 2021-09-14T11:36:20Z
date_published: 2021-07-02T00:00:00Z
date_updated: 2024-03-27T23:30:43Z
day: '02'
ddc:
- '575'
department:
- _id: JiFr
doi: 10.3390/cells10071665
ec_funded: 1
external_id:
isi:
- '000676604700001'
pmid:
- '34359847'
file:
- access_level: open_access
checksum: 2a9f534b9c2200e72e2cde95afaf4eed
content_type: application/pdf
creator: cchlebak
date_created: 2021-09-16T09:07:06Z
date_updated: 2021-09-16T09:07:06Z
file_id: '10021'
file_name: 2021_Cells_Nikonorova.pdf
file_size: 2667848
relation: main_file
success: 1
file_date_updated: 2021-09-16T09:07:06Z
has_accepted_license: '1'
intvolume: ' 10'
isi: 1
keyword:
- primary root
- (phospho)proteomics
- auxin
- (receptor) kinase
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
- _id: 3AC91DDA-15DF-11EA-824D-93A3E7B544D1
call_identifier: FWF
name: FWF Open Access Fund
publication: Cells
publication_identifier:
issn:
- 2073-4409
publication_status: published
publisher: MDPI
quality_controlled: '1'
related_material:
record:
- id: '10083'
relation: dissertation_contains
status: public
status: public
title: The Arabidopsis root tip (phospho)proteomes at growth-promoting versus growth-repressing
conditions reveal novel root growth regulators
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 10
year: '2021'
...
---
_id: '10095'
abstract:
- lang: eng
text: Growth regulation tailors plant development to its environment. A showcase
is response to gravity, where shoots bend up and roots down1. This paradox is
based on opposite effects of the phytohormone auxin, which promotes cell expansion
in shoots, while inhibiting it in roots via a yet unknown cellular mechanism2.
Here, by combining microfluidics, live imaging, genetic engineering and phospho-proteomics
in Arabidopsis thaliana, we advance our understanding how auxin inhibits root
growth. We show that auxin activates two distinct, antagonistically acting signalling
pathways that converge on the rapid regulation of the apoplastic pH, a causative
growth determinant. Cell surface-based TRANSMEMBRANE KINASE1 (TMK1) interacts
with and mediates phosphorylation and activation of plasma membrane H+-ATPases
for apoplast acidification, while intracellular canonical auxin signalling promotes
net cellular H+-influx, causing apoplast alkalinisation. The simultaneous activation
of these two counteracting mechanisms poises the root for a rapid, fine-tuned
growth modulation while navigating complex soil environment.
acknowledged_ssus:
- _id: LifeSc
- _id: M-Shop
- _id: Bio
acknowledgement: We thank Nataliia Gnyliukh and Lukas Hörmayer for technical assistance
and Nadine Paris for sharing PM-Cyto seeds. We gratefully acknowledge Life Science,
Machine Shop and Bioimaging Facilities of IST Austria. This project has received
funding from the European Research Council Advanced Grant (ETAP-742985) and the
Austrian Science Fund (FWF) I 3630-B25 to J.F., the National Institutes of Health
(GM067203) to W.M.G., the Netherlands Organization for Scientific Research (NWO;
VIDI-864.13.001.), the Research Foundation-Flanders (FWO; Odysseus II G0D0515N)
and a European Research Council Starting Grant (TORPEDO-714055) to W.S. and B.D.R.,
the VICI grant (865.14.001) from the Netherlands Organization for Scientific Research
to M.R and D.W., the Australian Research Council and China National Distinguished
Expert Project (WQ20174400441) to S.S., the MEXT/JSPS KAKENHI to K.T. (20K06685)
and T.K. (20H05687 and 20H05910), the European Union’s Horizon 2020 research and
innovation programme under the Marie Skłodowska-Curie Grant Agreement No. 665385
and the DOC Fellowship of the Austrian Academy of Sciences to L.L., the China Scholarship
Council to J.C.
article_number: '266395'
article_processing_charge: No
author:
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Mark
full_name: Roosjen, Mark
last_name: Roosjen
- first_name: Koji
full_name: Takahashi, Koji
last_name: Takahashi
- first_name: Lesia
full_name: Rodriguez Solovey, Lesia
id: 3922B506-F248-11E8-B48F-1D18A9856A87
last_name: Rodriguez Solovey
orcid: 0000-0002-7244-7237
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Jian
full_name: Chen, Jian
last_name: Chen
- first_name: Lana
full_name: Shabala, Lana
last_name: Shabala
- first_name: Wouter
full_name: Smet, Wouter
last_name: Smet
- first_name: Hong
full_name: Ren, Hong
last_name: Ren
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Sergey
full_name: Shabala, Sergey
last_name: Shabala
- first_name: Bert
full_name: De Rybel, Bert
last_name: De Rybel
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
- first_name: Toshinori
full_name: Kinoshita, Toshinori
last_name: Kinoshita
- first_name: William M.
full_name: Gray, William M.
last_name: Gray
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Li L, Verstraeten I, Roosjen M, et al. Cell surface and intracellular auxin
signalling for H+-fluxes in root growth. Research Square. doi:10.21203/rs.3.rs-266395/v3
apa: Li, L., Verstraeten, I., Roosjen, M., Takahashi, K., Rodriguez Solovey, L.,
Merrin, J., … Friml, J. (n.d.). Cell surface and intracellular auxin signalling
for H+-fluxes in root growth. Research Square. https://doi.org/10.21203/rs.3.rs-266395/v3
chicago: Li, Lanxin, Inge Verstraeten, Mark Roosjen, Koji Takahashi, Lesia Rodriguez
Solovey, Jack Merrin, Jian Chen, et al. “Cell Surface and Intracellular Auxin
Signalling for H+-Fluxes in Root Growth.” Research Square, n.d. https://doi.org/10.21203/rs.3.rs-266395/v3.
ieee: L. Li et al., “Cell surface and intracellular auxin signalling for
H+-fluxes in root growth,” Research Square. .
ista: Li L, Verstraeten I, Roosjen M, Takahashi K, Rodriguez Solovey L, Merrin J,
Chen J, Shabala L, Smet W, Ren H, Vanneste S, Shabala S, De Rybel B, Weijers D,
Kinoshita T, Gray WM, Friml J. Cell surface and intracellular auxin signalling
for H+-fluxes in root growth. Research Square, 266395.
mla: Li, Lanxin, et al. “Cell Surface and Intracellular Auxin Signalling for H+-Fluxes
in Root Growth.” Research Square, 266395, doi:10.21203/rs.3.rs-266395/v3.
short: L. Li, I. Verstraeten, M. Roosjen, K. Takahashi, L. Rodriguez Solovey, J.
Merrin, J. Chen, L. Shabala, W. Smet, H. Ren, S. Vanneste, S. Shabala, B. De Rybel,
D. Weijers, T. Kinoshita, W.M. Gray, J. Friml, Research Square (n.d.).
date_created: 2021-10-06T08:56:22Z
date_published: 2021-09-09T00:00:00Z
date_updated: 2024-03-27T23:30:43Z
day: '09'
department:
- _id: JiFr
- _id: NanoFab
doi: 10.21203/rs.3.rs-266395/v3
ec_funded: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.doi.org/10.21203/rs.3.rs-266395/v3
month: '09'
oa: 1
oa_version: Preprint
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 26B4D67E-B435-11E9-9278-68D0E5697425
grant_number: '25351'
name: 'A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated
Rapid Growth Inhibition in Arabidopsis Root'
publication: Research Square
publication_identifier:
issn:
- 2693-5015
publication_status: accepted
related_material:
record:
- id: '10223'
relation: later_version
status: public
- id: '10083'
relation: dissertation_contains
status: public
status: public
title: Cell surface and intracellular auxin signalling for H+-fluxes in root growth
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: preprint
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2021'
...
---
_id: '7601'
abstract:
- lang: eng
text: Plasmodesmata (PD) are crucial structures for intercellular communication
in multicellular plants with remorins being their crucial plant-specific structural
and functional constituents. The PD biogenesis is an intriguing but poorly understood
process. By expressing an Arabidopsis remorin protein in mammalian cells, we have
reconstituted a PD-like filamentous structure, termed remorin filament (RF), connecting
neighboring cells physically and physiologically. Notably, RFs are capable of
transporting macromolecules intercellularly, in a way similar to plant PD. With
further super-resolution microscopic analysis and biochemical characterization,
we found that RFs are also composed of actin filaments, forming the core skeleton
structure, aligned with the remorin protein. This unique heterologous filamentous
structure might explain the molecular mechanism for remorin function as well as
PD construction. Furthermore, remorin protein exhibits a specific distribution
manner in the plasma membrane in mammalian cells, representing a lipid nanodomain,
depending on its lipid modification status. Our studies not only provide crucial
insights into the mechanism of PD biogenesis, but also uncovers unsuspected fundamental
mechanistic and evolutionary links between intercellular communication systems
of plants and animals.
article_processing_charge: No
author:
- first_name: Zhuang
full_name: Wei, Zhuang
last_name: Wei
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Tao
full_name: Liu, Tao
last_name: Liu
- first_name: Yuan
full_name: Wu, Yuan
last_name: Wu
- first_name: Ji-Gang
full_name: Lei, Ji-Gang
last_name: Lei
- first_name: ZhengJun
full_name: Chen, ZhengJun
last_name: Chen
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Hong-Wei
full_name: Xue, Hong-Wei
last_name: Xue
- first_name: Kan
full_name: Liao, Kan
last_name: Liao
citation:
ama: Wei Z, Tan S, Liu T, et al. Plasmodesmata-like intercellular connections by
plant remorin in animal cells. bioRxiv. 2020. doi:10.1101/791137
apa: Wei, Z., Tan, S., Liu, T., Wu, Y., Lei, J.-G., Chen, Z., … Liao, K. (2020).
Plasmodesmata-like intercellular connections by plant remorin in animal cells.
bioRxiv. Cold Spring Harbor Laboratory. https://doi.org/10.1101/791137
chicago: Wei, Zhuang, Shutang Tan, Tao Liu, Yuan Wu, Ji-Gang Lei, ZhengJun Chen,
Jiří Friml, Hong-Wei Xue, and Kan Liao. “Plasmodesmata-like Intercellular Connections
by Plant Remorin in Animal Cells.” BioRxiv. Cold Spring Harbor Laboratory,
2020. https://doi.org/10.1101/791137.
ieee: Z. Wei et al., “Plasmodesmata-like intercellular connections by plant
remorin in animal cells,” bioRxiv. Cold Spring Harbor Laboratory, 2020.
ista: Wei Z, Tan S, Liu T, Wu Y, Lei J-G, Chen Z, Friml J, Xue H-W, Liao K. 2020.
Plasmodesmata-like intercellular connections by plant remorin in animal cells.
bioRxiv, 10.1101/791137.
mla: Wei, Zhuang, et al. “Plasmodesmata-like Intercellular Connections by Plant
Remorin in Animal Cells.” BioRxiv, Cold Spring Harbor Laboratory, 2020,
doi:10.1101/791137.
short: Z. Wei, S. Tan, T. Liu, Y. Wu, J.-G. Lei, Z. Chen, J. Friml, H.-W. Xue, K.
Liao, BioRxiv (2020).
date_created: 2020-03-21T16:34:42Z
date_published: 2020-02-19T00:00:00Z
date_updated: 2021-01-12T08:14:26Z
day: '19'
department:
- _id: JiFr
doi: 10.1101/791137
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/791137
month: '02'
oa: 1
oa_version: Preprint
page: '22'
publication: bioRxiv
publication_status: published
publisher: Cold Spring Harbor Laboratory
status: public
title: Plasmodesmata-like intercellular connections by plant remorin in animal cells
type: preprint
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2020'
...
---
_id: '6997'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Zhang Y, Friml J. Auxin guides roots to avoid obstacles during gravitropic
growth. New Phytologist. 2020;225(3):1049-1052. doi:10.1111/nph.16203
apa: Zhang, Y., & Friml, J. (2020). Auxin guides roots to avoid obstacles during
gravitropic growth. New Phytologist. Wiley. https://doi.org/10.1111/nph.16203
chicago: Zhang, Yuzhou, and Jiří Friml. “Auxin Guides Roots to Avoid Obstacles during
Gravitropic Growth.” New Phytologist. Wiley, 2020. https://doi.org/10.1111/nph.16203.
ieee: Y. Zhang and J. Friml, “Auxin guides roots to avoid obstacles during gravitropic
growth,” New Phytologist, vol. 225, no. 3. Wiley, pp. 1049–1052, 2020.
ista: Zhang Y, Friml J. 2020. Auxin guides roots to avoid obstacles during gravitropic
growth. New Phytologist. 225(3), 1049–1052.
mla: Zhang, Yuzhou, and Jiří Friml. “Auxin Guides Roots to Avoid Obstacles during
Gravitropic Growth.” New Phytologist, vol. 225, no. 3, Wiley, 2020, pp.
1049–52, doi:10.1111/nph.16203.
short: Y. Zhang, J. Friml, New Phytologist 225 (2020) 1049–1052.
date_created: 2019-11-12T11:41:32Z
date_published: 2020-02-01T00:00:00Z
date_updated: 2023-08-17T14:01:49Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/nph.16203
ec_funded: 1
external_id:
isi:
- '000489638800001'
pmid:
- '31603260'
file:
- access_level: open_access
checksum: cd42ffdb381fd52812b9583d4d407139
content_type: application/pdf
creator: dernst
date_created: 2020-11-18T16:42:48Z
date_updated: 2020-11-18T16:42:48Z
file_id: '8772'
file_name: 2020_NewPhytologist_Zhang.pdf
file_size: 717345
relation: main_file
success: 1
file_date_updated: 2020-11-18T16:42:48Z
has_accepted_license: '1'
intvolume: ' 225'
isi: 1
issue: '3'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: 1049-1052
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
publication: New Phytologist
publication_identifier:
eissn:
- 1469-8137
issn:
- 0028-646x
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Auxin guides roots to avoid obstacles during gravitropic growth
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 225
year: '2020'
...
---
_id: '7204'
abstract:
- lang: eng
text: Plant root architecture dynamically adapts to various environmental conditions,
such as salt‐containing soil. The phytohormone abscisic acid (ABA) is involved
among others also in these developmental adaptations, but the underlying molecular
mechanism remains elusive. Here, a novel branch of the ABA signaling pathway in
Arabidopsis involving PYR/PYL/RCAR (abbreviated as PYLs) receptor‐protein phosphatase
2A (PP2A) complex that acts in parallel to the canonical PYLs‐protein phosphatase
2C (PP2C) mechanism is identified. The PYLs‐PP2A signaling modulates root gravitropism
and lateral root formation through regulating phytohormone auxin transport. In
optimal conditions, PYLs ABA receptor interacts with the catalytic subunits of
PP2A, increasing their phosphatase activity and thus counteracting PINOID (PID)
kinase‐mediated phosphorylation of PIN‐FORMED (PIN) auxin transporters. By contrast,
in salt and osmotic stress conditions, ABA binds to PYLs, inhibiting the PP2A
activity, which leads to increased PIN phosphorylation and consequently modulated
directional auxin transport leading to adapted root architecture. This work reveals
an adaptive mechanism that may flexibly adjust plant root growth to withstand
saline and osmotic stresses. It occurs via the cross‐talk between the stress hormone
ABA and the versatile developmental regulator auxin.
article_number: '1901455'
article_processing_charge: No
article_type: original
author:
- first_name: Yang
full_name: Li, Yang
last_name: Li
- first_name: Yaping
full_name: Wang, Yaping
last_name: Wang
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Zhen
full_name: Li, Zhen
last_name: Li
- first_name: Zhi
full_name: Yuan, Zhi
last_name: Yuan
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: David
full_name: Domjan, David
id: C684CD7A-257E-11EA-9B6F-D8588B4F947F
last_name: Domjan
orcid: 0000-0003-2267-106X
- first_name: Kai
full_name: Wang, Kai
last_name: Wang
- first_name: Wei
full_name: Xuan, Wei
last_name: Xuan
- first_name: Yan
full_name: Guo, Yan
last_name: Guo
- first_name: Zhizhong
full_name: Gong, Zhizhong
last_name: Gong
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Jing
full_name: Zhang, Jing
last_name: Zhang
citation:
ama: Li Y, Wang Y, Tan S, et al. Root growth adaptation is mediated by PYLs ABA
receptor-PP2A protein phosphatase complex. Advanced Science. 2020;7(3).
doi:10.1002/advs.201901455
apa: Li, Y., Wang, Y., Tan, S., Li, Z., Yuan, Z., Glanc, M., … Zhang, J. (2020).
Root growth adaptation is mediated by PYLs ABA receptor-PP2A protein phosphatase
complex. Advanced Science. Wiley. https://doi.org/10.1002/advs.201901455
chicago: Li, Yang, Yaping Wang, Shutang Tan, Zhen Li, Zhi Yuan, Matous Glanc, David
Domjan, et al. “Root Growth Adaptation Is Mediated by PYLs ABA Receptor-PP2A Protein
Phosphatase Complex.” Advanced Science. Wiley, 2020. https://doi.org/10.1002/advs.201901455.
ieee: Y. Li et al., “Root growth adaptation is mediated by PYLs ABA receptor-PP2A
protein phosphatase complex,” Advanced Science, vol. 7, no. 3. Wiley, 2020.
ista: Li Y, Wang Y, Tan S, Li Z, Yuan Z, Glanc M, Domjan D, Wang K, Xuan W, Guo
Y, Gong Z, Friml J, Zhang J. 2020. Root growth adaptation is mediated by PYLs
ABA receptor-PP2A protein phosphatase complex. Advanced Science. 7(3), 1901455.
mla: Li, Yang, et al. “Root Growth Adaptation Is Mediated by PYLs ABA Receptor-PP2A
Protein Phosphatase Complex.” Advanced Science, vol. 7, no. 3, 1901455,
Wiley, 2020, doi:10.1002/advs.201901455.
short: Y. Li, Y. Wang, S. Tan, Z. Li, Z. Yuan, M. Glanc, D. Domjan, K. Wang, W.
Xuan, Y. Guo, Z. Gong, J. Friml, J. Zhang, Advanced Science 7 (2020).
date_created: 2019-12-22T23:00:43Z
date_published: 2020-02-05T00:00:00Z
date_updated: 2023-08-17T14:13:17Z
day: '05'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1002/advs.201901455
external_id:
isi:
- '000501912800001'
pmid:
- '32042554'
file:
- access_level: open_access
checksum: 016eeab5860860af038e2da95ffe75c3
content_type: application/pdf
creator: dernst
date_created: 2020-02-24T14:29:54Z
date_updated: 2020-07-14T12:47:53Z
file_id: '7519'
file_name: 2020_AdvScience_Li.pdf
file_size: 3586924
relation: main_file
file_date_updated: 2020-07-14T12:47:53Z
has_accepted_license: '1'
intvolume: ' 7'
isi: 1
issue: '3'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
publication: Advanced Science
publication_identifier:
eissn:
- 2198-3844
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Root growth adaptation is mediated by PYLs ABA receptor-PP2A protein phosphatase
complex
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 7
year: '2020'
...
---
_id: '7142'
abstract:
- lang: eng
text: The phytohormone auxin acts as an amazingly versatile coordinator of plant
growth and development. With its morphogen-like properties, auxin controls sites
and timing of differentiation and/or growth responses both, in quantitative and
qualitative terms. Specificity in the auxin response depends largely on distinct
modes of signal transmission, by which individual cells perceive and convert auxin
signals into a remarkable diversity of responses. The best understood, or so-called
canonical mechanism of auxin perception ultimately results in variable adjustments
of the cellular transcriptome, via a short, nuclear signal transduction pathway.
Additional findings that accumulated over decades implied that an additional,
presumably, cell surface-based auxin perception mechanism mediates very rapid
cellular responses and decisively contributes to the cell's overall hormonal response.
Recent investigations into both, nuclear and cell surface auxin signalling challenged
this assumed partition of roles for different auxin signalling pathways and revealed
an unexpected complexity in transcriptional and non-transcriptional cellular responses
mediated by auxin.
acknowledgement: Research in J.F. laboratory is funded by the European Union's Horizon
2020 program (ERC grant agreement n° 742985); C.L. is supported by the Austrian
Science Fund (FWF grant P 31493).
article_processing_charge: No
article_type: original
author:
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Christian
full_name: Luschnig, Christian
last_name: Luschnig
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Gallei MC, Luschnig C, Friml J. Auxin signalling in growth: Schrödinger’s
cat out of the bag. Current Opinion in Plant Biology. 2020;53(2):43-49.
doi:10.1016/j.pbi.2019.10.003'
apa: 'Gallei, M. C., Luschnig, C., & Friml, J. (2020). Auxin signalling in growth:
Schrödinger’s cat out of the bag. Current Opinion in Plant Biology. Elsevier.
https://doi.org/10.1016/j.pbi.2019.10.003'
chicago: 'Gallei, Michelle C, Christian Luschnig, and Jiří Friml. “Auxin Signalling
in Growth: Schrödinger’s Cat out of the Bag.” Current Opinion in Plant Biology.
Elsevier, 2020. https://doi.org/10.1016/j.pbi.2019.10.003.'
ieee: 'M. C. Gallei, C. Luschnig, and J. Friml, “Auxin signalling in growth: Schrödinger’s
cat out of the bag,” Current Opinion in Plant Biology, vol. 53, no. 2.
Elsevier, pp. 43–49, 2020.'
ista: 'Gallei MC, Luschnig C, Friml J. 2020. Auxin signalling in growth: Schrödinger’s
cat out of the bag. Current Opinion in Plant Biology. 53(2), 43–49.'
mla: 'Gallei, Michelle C., et al. “Auxin Signalling in Growth: Schrödinger’s Cat
out of the Bag.” Current Opinion in Plant Biology, vol. 53, no. 2, Elsevier,
2020, pp. 43–49, doi:10.1016/j.pbi.2019.10.003.'
short: M.C. Gallei, C. Luschnig, J. Friml, Current Opinion in Plant Biology 53 (2020)
43–49.
date_created: 2019-12-02T12:05:26Z
date_published: 2020-02-01T00:00:00Z
date_updated: 2023-08-17T14:07:22Z
day: '01'
department:
- _id: JiFr
doi: 10.1016/j.pbi.2019.10.003
ec_funded: 1
external_id:
isi:
- '000521120600007'
pmid:
- '31760231'
intvolume: ' 53'
isi: 1
issue: '2'
language:
- iso: eng
month: '02'
oa_version: None
page: 43-49
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Current Opinion in Plant Biology
publication_identifier:
eissn:
- 1879-0356
issn:
- 1369-5266
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
record:
- id: '11626'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: 'Auxin signalling in growth: Schrödinger''s cat out of the bag'
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 53
year: '2020'
...
---
_id: '7219'
abstract:
- lang: eng
text: Root system architecture (RSA), governed by the phytohormone auxin, endows
plants with an adaptive advantage in particular environments. Using geographically
representative arabidopsis (Arabidopsis thaliana) accessions as a resource for
GWA mapping, Waidmann et al. and Ogura et al. recently identified two novel components
involved in modulating auxin-mediated RSA and conferring plant fitness in particular
habitats.
article_processing_charge: No
article_type: original
author:
- first_name: Guanghui
full_name: Xiao, Guanghui
last_name: Xiao
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
citation:
ama: 'Xiao G, Zhang Y. Adaptive growth: Shaping auxin-mediated root system architecture.
Trends in Plant Science. 2020;25(2):P121-123. doi:10.1016/j.tplants.2019.12.001'
apa: 'Xiao, G., & Zhang, Y. (2020). Adaptive growth: Shaping auxin-mediated
root system architecture. Trends in Plant Science. Elsevier. https://doi.org/10.1016/j.tplants.2019.12.001'
chicago: 'Xiao, Guanghui, and Yuzhou Zhang. “Adaptive Growth: Shaping Auxin-Mediated
Root System Architecture.” Trends in Plant Science. Elsevier, 2020. https://doi.org/10.1016/j.tplants.2019.12.001.'
ieee: 'G. Xiao and Y. Zhang, “Adaptive growth: Shaping auxin-mediated root system
architecture,” Trends in Plant Science, vol. 25, no. 2. Elsevier, pp. P121-123,
2020.'
ista: 'Xiao G, Zhang Y. 2020. Adaptive growth: Shaping auxin-mediated root system
architecture. Trends in Plant Science. 25(2), P121-123.'
mla: 'Xiao, Guanghui, and Yuzhou Zhang. “Adaptive Growth: Shaping Auxin-Mediated
Root System Architecture.” Trends in Plant Science, vol. 25, no. 2, Elsevier,
2020, pp. P121-123, doi:10.1016/j.tplants.2019.12.001.'
short: G. Xiao, Y. Zhang, Trends in Plant Science 25 (2020) P121-123.
date_created: 2019-12-29T23:00:48Z
date_published: 2020-02-01T00:00:00Z
date_updated: 2023-08-17T14:14:50Z
day: '01'
department:
- _id: JiFr
doi: 10.1016/j.tplants.2019.12.001
external_id:
isi:
- '000508637500001'
pmid:
- '31843370'
intvolume: ' 25'
isi: 1
issue: '2'
language:
- iso: eng
month: '02'
oa_version: None
page: P121-123
pmid: 1
publication: Trends in Plant Science
publication_identifier:
issn:
- '13601385'
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Adaptive growth: Shaping auxin-mediated root system architecture'
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 25
year: '2020'
...
---
_id: '7465'
abstract:
- lang: eng
text: The flexible development of plants is characterized by a high capacity for
post-embryonic organ formation and tissue regeneration, processes, which require
tightly regulated intercellular communication and coordinated tissue (re-)polarization.
The phytohormone auxin, the main driver for these processes, is able to establish
polarized auxin transport channels, which are characterized by the expression
and polar, subcellular localization of the PIN1 auxin transport proteins. These
channels are demarcating the position of future vascular strands necessary for
organ formation and tissue regeneration. Major progress has been made in the last
years to understand how PINs can change their polarity in different contexts and
thus guide auxin flow through the plant. However, it still remains elusive how
auxin mediates the establishment of auxin conducting channels and the formation
of vascular tissue and which cellular processes are involved. By the means of
sophisticated regeneration experiments combined with local auxin applications
in Arabidopsis thaliana inflorescence stems we show that (i) PIN subcellular dynamics,
(ii) PIN internalization by clathrin-mediated trafficking and (iii) an intact
actin cytoskeleton required for post-endocytic trafficking are indispensable for
auxin channel formation, de novo vascular formation and vascular regeneration
after wounding. These observations provide novel insights into cellular mechanism
of coordinated tissue polarization during auxin canalization.
article_number: '110414'
article_processing_charge: No
article_type: original
author:
- first_name: Ewa
full_name: Mazur, Ewa
last_name: Mazur
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
- first_name: Hélène S.
full_name: Robert, Hélène S.
last_name: Robert
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Mazur E, Gallei MC, Adamowski M, Han H, Robert HS, Friml J. Clathrin-mediated
trafficking and PIN trafficking are required for auxin canalization and vascular
tissue formation in Arabidopsis. Plant Science. 2020;293(4). doi:10.1016/j.plantsci.2020.110414
apa: Mazur, E., Gallei, M. C., Adamowski, M., Han, H., Robert, H. S., & Friml,
J. (2020). Clathrin-mediated trafficking and PIN trafficking are required for
auxin canalization and vascular tissue formation in Arabidopsis. Plant Science.
Elsevier. https://doi.org/10.1016/j.plantsci.2020.110414
chicago: Mazur, Ewa, Michelle C Gallei, Maciek Adamowski, Huibin Han, Hélène S.
Robert, and Jiří Friml. “Clathrin-Mediated Trafficking and PIN Trafficking Are
Required for Auxin Canalization and Vascular Tissue Formation in Arabidopsis.”
Plant Science. Elsevier, 2020. https://doi.org/10.1016/j.plantsci.2020.110414.
ieee: E. Mazur, M. C. Gallei, M. Adamowski, H. Han, H. S. Robert, and J. Friml,
“Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization
and vascular tissue formation in Arabidopsis,” Plant Science, vol. 293,
no. 4. Elsevier, 2020.
ista: Mazur E, Gallei MC, Adamowski M, Han H, Robert HS, Friml J. 2020. Clathrin-mediated
trafficking and PIN trafficking are required for auxin canalization and vascular
tissue formation in Arabidopsis. Plant Science. 293(4), 110414.
mla: Mazur, Ewa, et al. “Clathrin-Mediated Trafficking and PIN Trafficking Are Required
for Auxin Canalization and Vascular Tissue Formation in Arabidopsis.” Plant
Science, vol. 293, no. 4, 110414, Elsevier, 2020, doi:10.1016/j.plantsci.2020.110414.
short: E. Mazur, M.C. Gallei, M. Adamowski, H. Han, H.S. Robert, J. Friml, Plant
Science 293 (2020).
date_created: 2020-02-09T23:00:50Z
date_published: 2020-04-01T00:00:00Z
date_updated: 2023-08-17T14:37:32Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.plantsci.2020.110414
ec_funded: 1
external_id:
isi:
- '000520609800009'
file:
- access_level: open_access
checksum: f7f27c6a8fea985ceb9279be2204461c
content_type: application/pdf
creator: dernst
date_created: 2020-02-10T08:59:36Z
date_updated: 2020-07-14T12:47:59Z
file_id: '7471'
file_name: 2020_PlantScience_Mazur.pdf
file_size: 3499069
relation: main_file
file_date_updated: 2020-07-14T12:47:59Z
has_accepted_license: '1'
intvolume: ' 293'
isi: 1
issue: '4'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Plant Science
publication_identifier:
eissn:
- '18732259'
issn:
- '01689452'
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
record:
- id: '11626'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization
and vascular tissue formation in Arabidopsis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 293
year: '2020'
...
---
_id: '7490'
abstract:
- lang: eng
text: In plants, clathrin mediated endocytosis (CME) represents the major route
for cargo internalisation from the cell surface. It has been assumed to operate
in an evolutionary conserved manner as in yeast and animals. Here we report characterisation
of ultrastructure, dynamics and mechanisms of plant CME as allowed by our advancement
in electron microscopy and quantitative live imaging techniques. Arabidopsis CME
appears to follow the constant curvature model and the bona fide CME population
generates vesicles of a predominantly hexagonal-basket type; larger and with faster
kinetics than in other models. Contrary to the existing paradigm, actin is dispensable
for CME events at the plasma membrane but plays a unique role in collecting endocytic
vesicles, sorting of internalised cargos and directional endosome movement that
itself actively promote CME events. Internalized vesicles display a strongly delayed
and sequential uncoating. These unique features highlight the independent evolution
of the plant CME mechanism during the autonomous rise of multicellularity in eukaryotes.
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
- _id: EM-Fac
article_number: e52067
article_processing_charge: No
article_type: original
author:
- first_name: Madhumitha
full_name: Narasimhan, Madhumitha
id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
last_name: Narasimhan
orcid: 0000-0002-8600-0671
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Roshan
full_name: Prizak, Roshan
id: 4456104E-F248-11E8-B48F-1D18A9856A87
last_name: Prizak
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Barbara E
full_name: Casillas Perez, Barbara E
id: 351ED2AA-F248-11E8-B48F-1D18A9856A87
last_name: Casillas Perez
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Narasimhan M, Johnson AJ, Prizak R, et al. Evolutionarily unique mechanistic
framework of clathrin-mediated endocytosis in plants. eLife. 2020;9. doi:10.7554/eLife.52067
apa: Narasimhan, M., Johnson, A. J., Prizak, R., Kaufmann, W., Tan, S., Casillas
Perez, B. E., & Friml, J. (2020). Evolutionarily unique mechanistic framework
of clathrin-mediated endocytosis in plants. ELife. eLife Sciences Publications.
https://doi.org/10.7554/eLife.52067
chicago: Narasimhan, Madhumitha, Alexander J Johnson, Roshan Prizak, Walter Kaufmann,
Shutang Tan, Barbara E Casillas Perez, and Jiří Friml. “Evolutionarily Unique
Mechanistic Framework of Clathrin-Mediated Endocytosis in Plants.” ELife.
eLife Sciences Publications, 2020. https://doi.org/10.7554/eLife.52067.
ieee: M. Narasimhan et al., “Evolutionarily unique mechanistic framework
of clathrin-mediated endocytosis in plants,” eLife, vol. 9. eLife Sciences
Publications, 2020.
ista: Narasimhan M, Johnson AJ, Prizak R, Kaufmann W, Tan S, Casillas Perez BE,
Friml J. 2020. Evolutionarily unique mechanistic framework of clathrin-mediated
endocytosis in plants. eLife. 9, e52067.
mla: Narasimhan, Madhumitha, et al. “Evolutionarily Unique Mechanistic Framework
of Clathrin-Mediated Endocytosis in Plants.” ELife, vol. 9, e52067, eLife
Sciences Publications, 2020, doi:10.7554/eLife.52067.
short: M. Narasimhan, A.J. Johnson, R. Prizak, W. Kaufmann, S. Tan, B.E. Casillas
Perez, J. Friml, ELife 9 (2020).
date_created: 2020-02-16T23:00:50Z
date_published: 2020-01-23T00:00:00Z
date_updated: 2023-08-18T06:33:07Z
day: '23'
ddc:
- '570'
- '580'
department:
- _id: JiFr
- _id: GaTk
- _id: EM-Fac
- _id: SyCr
doi: 10.7554/eLife.52067
ec_funded: 1
external_id:
isi:
- '000514104100001'
pmid:
- '31971511'
file:
- access_level: open_access
checksum: 2052daa4be5019534f3a42f200a09f32
content_type: application/pdf
creator: dernst
date_created: 2020-02-18T07:21:16Z
date_updated: 2020-07-14T12:47:59Z
file_id: '7494'
file_name: 2020_eLife_Narasimhan.pdf
file_size: 7247468
relation: main_file
file_date_updated: 2020-07-14T12:47:59Z
has_accepted_license: '1'
intvolume: ' 9'
isi: 1
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: eLife
publication_identifier:
eissn:
- 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: Evolutionarily unique mechanistic framework of clathrin-mediated endocytosis
in plants
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 9
year: '2020'
...
---
_id: '7497'
abstract:
- lang: eng
text: Endophytic fungi can be beneficial to plant growth. However, the molecular
mechanisms underlying colonization of Acremonium spp. remain unclear. In this
study, a novel endophytic Acremonium strain was isolated from the buds of Panax
notoginseng and named Acremonium sp. D212. The Acremonium sp. D212 could colonize
the roots of P. notoginseng, enhance the resistance of P. notoginseng to root
rot disease, and promote root growth and saponin biosynthesis in P. notoginseng.
Acremonium sp. D212 could secrete indole‐3‐acetic acid (IAA) and jasmonic acid
(JA), and inoculation with the fungus increased the endogenous levels of IAA and
JA in P. notoginseng. Colonization of the Acremonium sp. D212 in the roots of
the rice line Nipponbare was dependent on the concentration of methyl jasmonate
(MeJA) (2 to 15 μM) and 1‐naphthalenacetic acid (NAA) (10 to 20 μM). Moreover,
the roots of the JA signalling‐defective coi1‐18 mutant were colonized by Acremonium
sp. D212 to a lesser degree than those of the wild‐type Nipponbare and miR393b‐overexpressing
lines, and the colonization was rescued by MeJA but not by NAA. It suggests that
the cross‐talk between JA signalling and the auxin biosynthetic pathway plays
a crucial role in the colonization of Acremonium sp. D212 in host plants.
acknowledgement: We thank Professor Jianqiang Wu (Kunming Institute of Botany, Chinese
Academy of Sciences) for providing generous support with the IAA and JA measurements.
We thank Professor Guohua Xu (Nanjing Agricultural University) for generously providing
the Nipponbare rice expressing DR5::GUS. We thank Professor Muyuan Zhu (Zhejiang
University) for generously providing a rice line expressing 35S::miR393b. We thank
Professor Yinong Yang (Pennsylvania State University) for generously providing the
rice line coi1-18. This work was supported by grants from the National Natural Science
Foundation of China (31660501, 31460453, 31860064 and 31470382), the Major Special
Program for Scientific Research, Education Department of Yunnan Province (ZD2015005),
the Project sponsored by SRF for ROCS, SEM ([2013] 1792), the Major Science and
Technique Programs in Yunnan Province (2016ZF001), the Key Projects of the Applied
Basic Research Plan of Yunnan Province (2017FA018), the National Key R&D Program
of China (2018YFD0201100) and the China Agriculture Research System (CARS-21).
article_processing_charge: No
article_type: original
author:
- first_name: L
full_name: Han, L
last_name: Han
- first_name: X
full_name: Zhou, X
last_name: Zhou
- first_name: Y
full_name: Zhao, Y
last_name: Zhao
- first_name: S
full_name: Zhu, S
last_name: Zhu
- first_name: L
full_name: Wu, L
last_name: Wu
- first_name: Y
full_name: He, Y
last_name: He
- first_name: X
full_name: Ping, X
last_name: Ping
- first_name: X
full_name: Lu, X
last_name: Lu
- first_name: W
full_name: Huang, W
last_name: Huang
- first_name: J
full_name: Qian, J
last_name: Qian
- first_name: L
full_name: Zhang, L
last_name: Zhang
- first_name: X
full_name: Jiang, X
last_name: Jiang
- first_name: D
full_name: Zhu, D
last_name: Zhu
- first_name: C
full_name: Luo, C
last_name: Luo
- first_name: S
full_name: Li, S
last_name: Li
- first_name: Q
full_name: Dong, Q
last_name: Dong
- first_name: Q
full_name: Fu, Q
last_name: Fu
- first_name: K
full_name: Deng, K
last_name: Deng
- first_name: X
full_name: Wang, X
last_name: Wang
- first_name: L
full_name: Wang, L
last_name: Wang
- first_name: S
full_name: Peng, S
last_name: Peng
- first_name: J
full_name: Wu, J
last_name: Wu
- first_name: W
full_name: Li, W
last_name: Li
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Y
full_name: Zhu, Y
last_name: Zhu
- first_name: X
full_name: He, X
last_name: He
- first_name: Y
full_name: Du, Y
last_name: Du
citation:
ama: Han L, Zhou X, Zhao Y, et al. Colonization of endophyte Acremonium sp. D212
in Panax notoginseng and rice mediated by auxin and jasmonic acid. Journal
of Integrative Plant Biology. 2020;62(9):1433-1451. doi:10.1111/jipb.12905
apa: Han, L., Zhou, X., Zhao, Y., Zhu, S., Wu, L., He, Y., … Du, Y. (2020). Colonization
of endophyte Acremonium sp. D212 in Panax notoginseng and rice mediated by auxin
and jasmonic acid. Journal of Integrative Plant Biology. Wiley. https://doi.org/10.1111/jipb.12905
chicago: Han, L, X Zhou, Y Zhao, S Zhu, L Wu, Y He, X Ping, et al. “Colonization
of Endophyte Acremonium Sp. D212 in Panax Notoginseng and Rice Mediated by Auxin
and Jasmonic Acid.” Journal of Integrative Plant Biology. Wiley, 2020.
https://doi.org/10.1111/jipb.12905.
ieee: L. Han et al., “Colonization of endophyte Acremonium sp. D212 in Panax
notoginseng and rice mediated by auxin and jasmonic acid,” Journal of Integrative
Plant Biology, vol. 62, no. 9. Wiley, pp. 1433–1451, 2020.
ista: Han L, Zhou X, Zhao Y, Zhu S, Wu L, He Y, Ping X, Lu X, Huang W, Qian J, Zhang
L, Jiang X, Zhu D, Luo C, Li S, Dong Q, Fu Q, Deng K, Wang X, Wang L, Peng S,
Wu J, Li W, Friml J, Zhu Y, He X, Du Y. 2020. Colonization of endophyte Acremonium
sp. D212 in Panax notoginseng and rice mediated by auxin and jasmonic acid. Journal
of Integrative Plant Biology. 62(9), 1433–1451.
mla: Han, L., et al. “Colonization of Endophyte Acremonium Sp. D212 in Panax Notoginseng
and Rice Mediated by Auxin and Jasmonic Acid.” Journal of Integrative Plant
Biology, vol. 62, no. 9, Wiley, 2020, pp. 1433–51, doi:10.1111/jipb.12905.
short: L. Han, X. Zhou, Y. Zhao, S. Zhu, L. Wu, Y. He, X. Ping, X. Lu, W. Huang,
J. Qian, L. Zhang, X. Jiang, D. Zhu, C. Luo, S. Li, Q. Dong, Q. Fu, K. Deng, X.
Wang, L. Wang, S. Peng, J. Wu, W. Li, J. Friml, Y. Zhu, X. He, Y. Du, Journal
of Integrative Plant Biology 62 (2020) 1433–1451.
date_created: 2020-02-18T10:02:25Z
date_published: 2020-09-01T00:00:00Z
date_updated: 2023-08-18T06:44:16Z
day: '01'
department:
- _id: JiFr
doi: 10.1111/jipb.12905
external_id:
isi:
- '000515803000001'
pmid:
- '31912615'
intvolume: ' 62'
isi: 1
issue: '9'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1111/jipb.12905
month: '09'
oa: 1
oa_version: Published Version
page: 1433-1451
pmid: 1
publication: Journal of Integrative Plant Biology
publication_identifier:
eissn:
- 1744-7909
issn:
- 1672-9072
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Colonization of endophyte Acremonium sp. D212 in Panax notoginseng and rice
mediated by auxin and jasmonic acid
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 62
year: '2020'
...
---
_id: '7540'
abstract:
- lang: eng
text: ' In vitro propagation of the ornamentally interesting species Wikstroemia
gemmata is limited by the recalcitrance to form adventitious roots. In this article,
two strategies to improve the rooting capacity of in vitro microcuttings are presented.
Firstly, the effect of exogenous auxin was evaluated in both light and dark cultivated
stem segments and also the sucrose-content of the medium was varied in order to
determine better rooting conditions. Secondly, different spectral lights were
evaluated and the effect on shoot growth and root induction demonstrated that
the exact spectral composition of light is important for successful in vitro growth
and development of Wikstroemia gemmata. We show that exogenous auxin cannot compensate
for the poor rooting under unfavorable light conditions. Adapting the culture
conditions is therefore paramount for successful industrial propagation of Wikstroemia
gemmata. '
article_processing_charge: No
article_type: original
author:
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: H.
full_name: Buyle, H.
last_name: Buyle
- first_name: S.
full_name: Werbrouck, S.
last_name: Werbrouck
- first_name: M.C.
full_name: Van Labeke, M.C.
last_name: Van Labeke
- first_name: D.
full_name: Geelen, D.
last_name: Geelen
citation:
ama: Verstraeten I, Buyle H, Werbrouck S, Van Labeke MC, Geelen D. In vitro shoot
growth and adventitious rooting of Wikstroemia gemmata depends on light quality.
Israel Journal of Plant Sciences. 2020;67(1-2):16-26. doi:10.1163/22238980-20191110
apa: Verstraeten, I., Buyle, H., Werbrouck, S., Van Labeke, M. C., & Geelen,
D. (2020). In vitro shoot growth and adventitious rooting of Wikstroemia gemmata
depends on light quality. Israel Journal of Plant Sciences. Brill. https://doi.org/10.1163/22238980-20191110
chicago: Verstraeten, Inge, H. Buyle, S. Werbrouck, M.C. Van Labeke, and D. Geelen.
“In Vitro Shoot Growth and Adventitious Rooting of Wikstroemia Gemmata Depends
on Light Quality.” Israel Journal of Plant Sciences. Brill, 2020. https://doi.org/10.1163/22238980-20191110.
ieee: I. Verstraeten, H. Buyle, S. Werbrouck, M. C. Van Labeke, and D. Geelen, “In
vitro shoot growth and adventitious rooting of Wikstroemia gemmata depends on
light quality,” Israel Journal of Plant Sciences, vol. 67, no. 1–2. Brill,
pp. 16–26, 2020.
ista: Verstraeten I, Buyle H, Werbrouck S, Van Labeke MC, Geelen D. 2020. In vitro
shoot growth and adventitious rooting of Wikstroemia gemmata depends on light
quality. Israel Journal of Plant Sciences. 67(1–2), 16–26.
mla: Verstraeten, Inge, et al. “In Vitro Shoot Growth and Adventitious Rooting of
Wikstroemia Gemmata Depends on Light Quality.” Israel Journal of Plant Sciences,
vol. 67, no. 1–2, Brill, 2020, pp. 16–26, doi:10.1163/22238980-20191110.
short: I. Verstraeten, H. Buyle, S. Werbrouck, M.C. Van Labeke, D. Geelen, Israel
Journal of Plant Sciences 67 (2020) 16–26.
date_created: 2020-02-28T09:18:01Z
date_published: 2020-02-01T00:00:00Z
date_updated: 2023-08-18T06:45:15Z
day: '01'
department:
- _id: JiFr
doi: 10.1163/22238980-20191110
external_id:
isi:
- '000525343300004'
intvolume: ' 67'
isi: 1
issue: 1-2
language:
- iso: eng
month: '02'
oa_version: None
page: 16-26
publication: Israel Journal of Plant Sciences
publication_identifier:
eissn:
- 2223-8980
issn:
- 0792-9978
publication_status: published
publisher: Brill
quality_controlled: '1'
scopus_import: '1'
status: public
title: In vitro shoot growth and adventitious rooting of Wikstroemia gemmata depends
on light quality
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 67
year: '2020'
...
---
_id: '7582'
abstract:
- lang: eng
text: Small RNAs (smRNA, 19–25 nucleotides long), which are transcribed by RNA polymerase
II, regulate the expression of genes involved in a multitude of processes in eukaryotes.
miRNA biogenesis and the proteins involved in the biogenesis pathway differ across
plant and animal lineages. The major proteins constituting the biogenesis pathway,
namely, the Dicers (DCL/DCR) and Argonautes (AGOs), have been extensively studied.
However, the accessory proteins (DAWDLE (DDL), SERRATE (SE), and TOUGH (TGH))
of the pathway that differs across the two lineages remain largely uncharacterized.
We present the first detailed report on the molecular evolution and divergence
of these proteins across eukaryotes. Although DDL is present in eukaryotes and
prokaryotes, SE and TGH appear to be specific to eukaryotes. The addition/deletion
of specific domains and/or domain-specific sequence divergence in the three proteins
points to the observed functional divergence of these proteins across the two
lineages, which correlates with the differences in miRNA length across the two
lineages. Our data enhance the current understanding of the structure–function
relationship of these proteins and reveals previous unexplored crucial residues
in the three proteins that can be used as a basis for further functional characterization.
The data presented here on the number of miRNAs in crown eukaryotic lineages are
consistent with the notion of the expansion of the number of miRNA-coding genes
in animal and plant lineages correlating with organismal complexity. Whether this
difference in functionally correlates with the diversification (or presence/absence)
of the three proteins studied here or the miRNA signaling in the plant and animal
lineages is unclear. Based on our results of the three proteins studied here and
previously available data concerning the evolution of miRNA genes in the plant
and animal lineages, we believe that miRNAs probably evolved once in the ancestor
to crown eukaryotes and have diversified independently in the eukaryotes.
article_number: '299'
article_processing_charge: No
article_type: original
author:
- first_name: Taraka Ramji
full_name: Moturu, Taraka Ramji
last_name: Moturu
- first_name: Sansrity
full_name: Sinha, Sansrity
last_name: Sinha
- first_name: Hymavathi
full_name: Salava, Hymavathi
last_name: Salava
- first_name: Sravankumar
full_name: Thula, Sravankumar
last_name: Thula
- first_name: Tomasz
full_name: Nodzyński, Tomasz
last_name: Nodzyński
- first_name: Radka Svobodová
full_name: Vařeková, Radka Svobodová
last_name: Vařeková
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Sibu
full_name: Simon, Sibu
id: 4542EF9A-F248-11E8-B48F-1D18A9856A87
last_name: Simon
orcid: 0000-0002-1998-6741
citation:
ama: Moturu TR, Sinha S, Salava H, et al. Molecular evolution and diversification
of proteins involved in miRNA maturation pathway. Plants. 2020;9(3). doi:10.3390/plants9030299
apa: Moturu, T. R., Sinha, S., Salava, H., Thula, S., Nodzyński, T., Vařeková, R.
S., … Simon, S. (2020). Molecular evolution and diversification of proteins involved
in miRNA maturation pathway. Plants. MDPI. https://doi.org/10.3390/plants9030299
chicago: Moturu, Taraka Ramji, Sansrity Sinha, Hymavathi Salava, Sravankumar Thula,
Tomasz Nodzyński, Radka Svobodová Vařeková, Jiří Friml, and Sibu Simon. “Molecular
Evolution and Diversification of Proteins Involved in MiRNA Maturation Pathway.”
Plants. MDPI, 2020. https://doi.org/10.3390/plants9030299.
ieee: T. R. Moturu et al., “Molecular evolution and diversification of proteins
involved in miRNA maturation pathway,” Plants, vol. 9, no. 3. MDPI, 2020.
ista: Moturu TR, Sinha S, Salava H, Thula S, Nodzyński T, Vařeková RS, Friml J,
Simon S. 2020. Molecular evolution and diversification of proteins involved in
miRNA maturation pathway. Plants. 9(3), 299.
mla: Moturu, Taraka Ramji, et al. “Molecular Evolution and Diversification of Proteins
Involved in MiRNA Maturation Pathway.” Plants, vol. 9, no. 3, 299, MDPI,
2020, doi:10.3390/plants9030299.
short: T.R. Moturu, S. Sinha, H. Salava, S. Thula, T. Nodzyński, R.S. Vařeková,
J. Friml, S. Simon, Plants 9 (2020).
date_created: 2020-03-15T23:00:52Z
date_published: 2020-03-01T00:00:00Z
date_updated: 2023-08-18T07:07:08Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.3390/plants9030299
ec_funded: 1
external_id:
isi:
- '000525315000035'
pmid:
- '32121542'
file:
- access_level: open_access
checksum: 6d5af3e17266a48996b4af4e67e88a85
content_type: application/pdf
creator: dernst
date_created: 2020-03-23T13:37:00Z
date_updated: 2020-07-14T12:48:00Z
file_id: '7614'
file_name: 2020_Plants_Moturu.pdf
file_size: 2373484
relation: main_file
file_date_updated: 2020-07-14T12:48:00Z
has_accepted_license: '1'
intvolume: ' 9'
isi: 1
issue: '3'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Plants
publication_identifier:
eissn:
- '22237747'
publication_status: published
publisher: MDPI
quality_controlled: '1'
scopus_import: '1'
status: public
title: Molecular evolution and diversification of proteins involved in miRNA maturation
pathway
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 9
year: '2020'
...
---
_id: '7600'
abstract:
- lang: eng
text: Directional intercellular transport of the phytohormone auxin mediated by
PIN FORMED (PIN) efflux carriers plays essential roles in both coordinating patterning
processes and integrating multiple external cues by rapidly redirecting auxin
fluxes. Multilevel regulations of PIN activity under internal and external cues
are complicated; however, the underlying molecular mechanism remains elusive.
Here we demonstrate that 3’-Phosphoinositide-Dependent Protein Kinase1 (PDK1),
which is conserved in plants and mammals, functions as a molecular hub integrating
the upstream lipid signalling and the downstream substrate activity through phosphorylation.
Genetic analysis uncovers that loss-of-function Arabidopsis mutant pdk1.1 pdk1.2
exhibits a plethora of abnormalities in organogenesis and growth, due to the defective
PIN-dependent auxin transport. Further cellular and biochemical analyses reveal
that PDK1 phosphorylates D6 Protein Kinase to facilitate its activity towards
PIN proteins. Our studies establish a lipid-dependent phosphorylation cascade
connecting membrane composition-based cellular signalling with plant growth and
patterning by regulating morphogenetic auxin fluxes.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
article_processing_charge: No
article_type: original
author:
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Xixi
full_name: Zhang, Xixi
id: 61A66458-47E9-11EA-85BA-8AEAAF14E49A
last_name: Zhang
orcid: 0000-0001-7048-4627
- first_name: Wei
full_name: Kong, Wei
last_name: Kong
- first_name: Xiao-Li
full_name: Yang, Xiao-Li
last_name: Yang
- first_name: Gergely
full_name: Molnar, Gergely
id: 34F1AF46-F248-11E8-B48F-1D18A9856A87
last_name: Molnar
- first_name: Zuzana
full_name: Vondráková, Zuzana
last_name: Vondráková
- first_name: Roberta
full_name: Filepová, Roberta
last_name: Filepová
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Hong-Wei
full_name: Xue, Hong-Wei
last_name: Xue
citation:
ama: Tan S, Zhang X, Kong W, et al. The lipid code-dependent phosphoswitch PDK1–D6PK
activates PIN-mediated auxin efflux in Arabidopsis. Nature Plants. 2020;6:556-569.
doi:10.1038/s41477-020-0648-9
apa: Tan, S., Zhang, X., Kong, W., Yang, X.-L., Molnar, G., Vondráková, Z., … Xue,
H.-W. (2020). The lipid code-dependent phosphoswitch PDK1–D6PK activates PIN-mediated
auxin efflux in Arabidopsis. Nature Plants. Springer Nature. https://doi.org/10.1038/s41477-020-0648-9
chicago: Tan, Shutang, Xixi Zhang, Wei Kong, Xiao-Li Yang, Gergely Molnar, Zuzana
Vondráková, Roberta Filepová, Jan Petrášek, Jiří Friml, and Hong-Wei Xue. “The
Lipid Code-Dependent Phosphoswitch PDK1–D6PK Activates PIN-Mediated Auxin Efflux
in Arabidopsis.” Nature Plants. Springer Nature, 2020. https://doi.org/10.1038/s41477-020-0648-9.
ieee: S. Tan et al., “The lipid code-dependent phosphoswitch PDK1–D6PK activates
PIN-mediated auxin efflux in Arabidopsis,” Nature Plants, vol. 6. Springer
Nature, pp. 556–569, 2020.
ista: Tan S, Zhang X, Kong W, Yang X-L, Molnar G, Vondráková Z, Filepová R, Petrášek
J, Friml J, Xue H-W. 2020. The lipid code-dependent phosphoswitch PDK1–D6PK activates
PIN-mediated auxin efflux in Arabidopsis. Nature Plants. 6, 556–569.
mla: Tan, Shutang, et al. “The Lipid Code-Dependent Phosphoswitch PDK1–D6PK Activates
PIN-Mediated Auxin Efflux in Arabidopsis.” Nature Plants, vol. 6, Springer
Nature, 2020, pp. 556–69, doi:10.1038/s41477-020-0648-9.
short: S. Tan, X. Zhang, W. Kong, X.-L. Yang, G. Molnar, Z. Vondráková, R. Filepová,
J. Petrášek, J. Friml, H.-W. Xue, Nature Plants 6 (2020) 556–569.
date_created: 2020-03-21T16:34:16Z
date_published: 2020-05-01T00:00:00Z
date_updated: 2023-08-18T07:05:57Z
day: '01'
department:
- _id: JiFr
doi: 10.1038/s41477-020-0648-9
ec_funded: 1
external_id:
isi:
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pmid:
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intvolume: ' 6'
isi: 1
language:
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url: https://doi.org/10.1101/755504
month: '05'
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page: 556-569
pmid: 1
project:
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call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
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grant_number: 723-2015
name: Long Term Fellowship
publication: Nature Plants
publication_identifier:
eissn:
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publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- relation: erratum
url: https://doi.org/10.1038/s41477-020-0719-y
scopus_import: '1'
status: public
title: The lipid code-dependent phosphoswitch PDK1–D6PK activates PIN-mediated auxin
efflux in Arabidopsis
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 6
year: '2020'
...