---
_id: '913'
abstract:
- lang: eng
text: Coordinated cell polarization in developing tissues is a recurrent theme in
multicellular organisms. In plants, a directional distribution of the plant hormone
auxin is at the core of many developmental programs. A feedback regulation of
auxin on the polarized localization of PIN auxin transporters in individual cells
has been proposed as a self-organizing mechanism for coordinated tissue polarization,
but the molecular mechanisms linking auxin signalling to PIN-dependent auxin transport
remain unknown. We performed a microarray-based approach to find regulators of
the auxin-induced PIN relocation in the Arabidopsis thaliana root. We identified
a subset of a family of phosphatidylinositol transfer proteins (PITP), the PATELLINs
(PATL). Here, we show that PATLs are expressed in partially overlapping cells
types in different tissues going through mitosis or initiating differentiation
programs. PATLs are plasma membrane-associated proteins accumulated in Arabidopsis
embryos, primary roots, lateral root primordia, and developing stomata. Higher
order patl mutants display reduced PIN1 repolarization in response to auxin, shorter
root apical meristem, and drastic defects in embryo and seedling development.
This suggests PATLs redundantly play a crucial role in polarity and patterning
in Arabidopsis.
article_number: jcs.204198
article_processing_charge: No
author:
- first_name: Ricardo
full_name: Tejos, Ricardo
last_name: Tejos
- first_name: Cecilia
full_name: Rodríguez Furlán, Cecilia
last_name: Rodríguez Furlán
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Michael
full_name: Sauer, Michael
last_name: Sauer
- first_name: Lorena
full_name: Norambuena, Lorena
last_name: Norambuena
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Tejos R, Rodríguez Furlán C, Adamowski M, Sauer M, Norambuena L, Friml J. PATELLINS
are regulators of auxin mediated PIN1 relocation and plant development in Arabidopsis
thaliana. Journal of Cell Science. 2018;131(2). doi:10.1242/jcs.204198
apa: Tejos, R., Rodríguez Furlán, C., Adamowski, M., Sauer, M., Norambuena, L.,
& Friml, J. (2018). PATELLINS are regulators of auxin mediated PIN1 relocation
and plant development in Arabidopsis thaliana. Journal of Cell Science.
Company of Biologists. https://doi.org/10.1242/jcs.204198
chicago: Tejos, Ricardo, Cecilia Rodríguez Furlán, Maciek Adamowski, Michael Sauer,
Lorena Norambuena, and Jiří Friml. “PATELLINS Are Regulators of Auxin Mediated
PIN1 Relocation and Plant Development in Arabidopsis Thaliana.” Journal of
Cell Science. Company of Biologists, 2018. https://doi.org/10.1242/jcs.204198.
ieee: R. Tejos, C. Rodríguez Furlán, M. Adamowski, M. Sauer, L. Norambuena, and
J. Friml, “PATELLINS are regulators of auxin mediated PIN1 relocation and plant
development in Arabidopsis thaliana,” Journal of Cell Science, vol. 131,
no. 2. Company of Biologists, 2018.
ista: Tejos R, Rodríguez Furlán C, Adamowski M, Sauer M, Norambuena L, Friml J.
2018. PATELLINS are regulators of auxin mediated PIN1 relocation and plant development
in Arabidopsis thaliana. Journal of Cell Science. 131(2), jcs. 204198.
mla: Tejos, Ricardo, et al. “PATELLINS Are Regulators of Auxin Mediated PIN1 Relocation
and Plant Development in Arabidopsis Thaliana.” Journal of Cell Science,
vol. 131, no. 2, jcs. 204198, Company of Biologists, 2018, doi:10.1242/jcs.204198.
short: R. Tejos, C. Rodríguez Furlán, M. Adamowski, M. Sauer, L. Norambuena, J.
Friml, Journal of Cell Science 131 (2018).
date_created: 2018-12-11T11:49:10Z
date_published: 2018-01-29T00:00:00Z
date_updated: 2023-09-26T15:47:50Z
day: '29'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.1242/jcs.204198
ec_funded: 1
external_id:
isi:
- '000424842400019'
file:
- access_level: open_access
checksum: bf156c20a4f117b4b932370d54cbac8c
content_type: application/pdf
creator: dernst
date_created: 2019-04-12T08:46:32Z
date_updated: 2020-07-14T12:48:15Z
file_id: '6299'
file_name: 2017_adamowski_PATELLINS_are.pdf
file_size: 14925985
relation: main_file
file_date_updated: 2020-07-14T12:48:15Z
has_accepted_license: '1'
intvolume: ' 131'
isi: 1
issue: '2'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Journal of Cell Science
publication_identifier:
issn:
- '00219533'
publication_status: published
publisher: Company of Biologists
publist_id: '6530'
pubrep_id: '988'
quality_controlled: '1'
scopus_import: '1'
status: public
title: PATELLINS are regulators of auxin mediated PIN1 relocation and plant development
in Arabidopsis thaliana
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 131
year: '2018'
...
---
_id: '5673'
abstract:
- lang: eng
text: Cell polarity, manifested by the localization of proteins to distinct polar
plasma membrane domains, is a key prerequisite of multicellular life. In plants,
PIN auxin transporters are prominent polarity markers crucial for a plethora of
developmental processes. Cell polarity mechanisms in plants are distinct from
other eukaryotes and still largely elusive. In particular, how the cell polarities
are propagated and maintained following cell division remains unknown. Plant cytokinesis
is orchestrated by the cell plate—a transient centrifugally growing endomembrane
compartment ultimately forming the cross wall1. Trafficking of polar membrane
proteins is typically redirected to the cell plate, and these will consequently
have opposite polarity in at least one of the daughter cells2–5. Here, we provide
mechanistic insights into post-cytokinetic re-establishment of cell polarity as
manifested by the apical, polar localization of PIN2. We show that the apical
domain is defined in a cell-intrinsic manner and that re-establishment of PIN2
localization to this domain requires de novo protein secretion and endocytosis,
but not basal-to-apical transcytosis. Furthermore, we identify a PINOID-related
kinase WAG1, which phosphorylates PIN2 in vitro6 and is transcriptionally upregulated
specifically in dividing cells, as a crucial regulator of post-cytokinetic PIN2
polarity re-establishment.
article_processing_charge: No
author:
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Glanc M, Fendrych M, Friml J. Mechanistic framework for cell-intrinsic re-establishment
of PIN2 polarity after cell division. Nature Plants. 2018;4(12):1082-1088.
doi:10.1038/s41477-018-0318-3
apa: Glanc, M., Fendrych, M., & Friml, J. (2018). Mechanistic framework for
cell-intrinsic re-establishment of PIN2 polarity after cell division. Nature
Plants. Nature Research. https://doi.org/10.1038/s41477-018-0318-3
chicago: Glanc, Matous, Matyas Fendrych, and Jiří Friml. “Mechanistic Framework
for Cell-Intrinsic Re-Establishment of PIN2 Polarity after Cell Division.” Nature
Plants. Nature Research, 2018. https://doi.org/10.1038/s41477-018-0318-3.
ieee: M. Glanc, M. Fendrych, and J. Friml, “Mechanistic framework for cell-intrinsic
re-establishment of PIN2 polarity after cell division,” Nature Plants,
vol. 4, no. 12. Nature Research, pp. 1082–1088, 2018.
ista: Glanc M, Fendrych M, Friml J. 2018. Mechanistic framework for cell-intrinsic
re-establishment of PIN2 polarity after cell division. Nature Plants. 4(12), 1082–1088.
mla: Glanc, Matous, et al. “Mechanistic Framework for Cell-Intrinsic Re-Establishment
of PIN2 Polarity after Cell Division.” Nature Plants, vol. 4, no. 12, Nature
Research, 2018, pp. 1082–88, doi:10.1038/s41477-018-0318-3.
short: M. Glanc, M. Fendrych, J. Friml, Nature Plants 4 (2018) 1082–1088.
date_created: 2018-12-16T22:59:18Z
date_published: 2018-12-03T00:00:00Z
date_updated: 2023-10-17T12:19:28Z
day: '03'
department:
- _id: JiFr
doi: 10.1038/s41477-018-0318-3
ec_funded: 1
external_id:
isi:
- '000454576600017'
pmid:
- '30518833'
intvolume: ' 4'
isi: 1
issue: '12'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pubmed/30518833
month: '12'
oa: 1
oa_version: Submitted Version
page: 1082-1088
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Nature Plants
publication_identifier:
issn:
- 2055-0278
publication_status: published
publisher: Nature Research
quality_controlled: '1'
scopus_import: '1'
status: public
title: Mechanistic framework for cell-intrinsic re-establishment of PIN2 polarity
after cell division
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 4
year: '2018'
...
---
_id: '412'
abstract:
- lang: eng
text: Clathrin-mediated endocytosis (CME) is a cellular trafficking process in which
cargoes and lipids are internalized from the plasma membrane into vesicles coated
with clathrin and adaptor proteins. CME is essential for many developmental and
physiological processes in plants, but its underlying mechanism is not well characterised
compared to that in yeast and animal systems. Here, we searched for new factors
involved in CME in Arabidopsis thaliana by performing Tandem Affinity Purification
of proteins that interact with clathrin light chain, a principal component of
the clathrin coat. Among the confirmed interactors, we found two putative homologues
of the clathrin-coat uncoating factor auxilin previously described in non-plant
systems. Overexpression of AUXILIN-LIKE1 and AUXILIN-LIKE2 in A. thaliana caused
an arrest of seedling growth and development. This was concomitant with inhibited
endocytosis due to blocking of clathrin recruitment after the initial step of
adaptor protein binding to the plasma membrane. By contrast, auxilin-like(1/2)
loss-of-function lines did not present endocytosis-related developmental or cellular
phenotypes under normal growth conditions. This work contributes to the on-going
characterization of the endocytotic machinery in plants and provides a robust
tool for conditionally and specifically interfering with CME in A. thaliana.
acknowledgement: We thank James Matthew Watson, Monika Borowska, and Peggy Stolt-Bergner
at ProTech Facility of the Vienna Biocenter Core Facilities for the CRISPR/CAS9
construct; Anna Müller for assistance with molecular cloning; Sebastian Bednarek,
Liwen Jiang, and Daniël Van Damme for sharing published material; Matyáš Fendrych,
Daniël Van Damme, and Lindy Abas for valuable discussions; and Martine De Cock for
help with correcting the manuscript. This work was supported by the European Research
Council under the European Union Seventh Framework Programme (FP7/2007-2013)/ERC
Grant 282300 and by the Ministry of Education of the Czech Republic/MŠMT project
NPUI-LO1417.
article_processing_charge: No
article_type: original
author:
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Madhumitha
full_name: Narasimhan, Madhumitha
id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
last_name: Narasimhan
orcid: 0000-0002-8600-0671
- first_name: Urszula
full_name: Kania, Urszula
id: 4AE5C486-F248-11E8-B48F-1D18A9856A87
last_name: Kania
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: Geert
full_name: De Jaeger, Geert
last_name: De Jaeger
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Adamowski M, Narasimhan M, Kania U, Glanc M, De Jaeger G, Friml J. A functional
study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors in Arabidopsis.
The Plant Cell. 2018;30(3):700-716. doi:10.1105/tpc.17.00785
apa: Adamowski, M., Narasimhan, M., Kania, U., Glanc, M., De Jaeger, G., & Friml,
J. (2018). A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating
factors in Arabidopsis. The Plant Cell. American Society of Plant Biologists.
https://doi.org/10.1105/tpc.17.00785
chicago: Adamowski, Maciek, Madhumitha Narasimhan, Urszula Kania, Matous Glanc,
Geert De Jaeger, and Jiří Friml. “A Functional Study of AUXILIN LIKE1 and 2 Two
Putative Clathrin Uncoating Factors in Arabidopsis.” The Plant Cell. American
Society of Plant Biologists, 2018. https://doi.org/10.1105/tpc.17.00785.
ieee: M. Adamowski, M. Narasimhan, U. Kania, M. Glanc, G. De Jaeger, and J. Friml,
“A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors
in Arabidopsis,” The Plant Cell, vol. 30, no. 3. American Society of Plant
Biologists, pp. 700–716, 2018.
ista: Adamowski M, Narasimhan M, Kania U, Glanc M, De Jaeger G, Friml J. 2018. A
functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors
in Arabidopsis. The Plant Cell. 30(3), 700–716.
mla: Adamowski, Maciek, et al. “A Functional Study of AUXILIN LIKE1 and 2 Two Putative
Clathrin Uncoating Factors in Arabidopsis.” The Plant Cell, vol. 30, no.
3, American Society of Plant Biologists, 2018, pp. 700–16, doi:10.1105/tpc.17.00785.
short: M. Adamowski, M. Narasimhan, U. Kania, M. Glanc, G. De Jaeger, J. Friml,
The Plant Cell 30 (2018) 700–716.
date_created: 2018-12-11T11:46:20Z
date_published: 2018-04-09T00:00:00Z
date_updated: 2024-03-28T23:30:06Z
day: '09'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1105/tpc.17.00785
ec_funded: 1
external_id:
isi:
- '000429441400018'
pmid:
- '29511054'
file:
- access_level: open_access
checksum: 4e165e653b67d3f0684697f21aace5a1
content_type: application/pdf
creator: dernst
date_created: 2022-05-23T09:12:38Z
date_updated: 2022-05-23T09:12:38Z
file_id: '11406'
file_name: 2018_PlantCell_Adamowski.pdf
file_size: 4407538
relation: main_file
success: 1
file_date_updated: 2022-05-23T09:12:38Z
has_accepted_license: '1'
intvolume: ' 30'
isi: 1
issue: '3'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
page: 700 - 716
pmid: 1
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: The Plant Cell
publication_identifier:
eissn:
- 1532-298X
issn:
- 1040-4651
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '7417'
quality_controlled: '1'
related_material:
record:
- id: '6269'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors
in Arabidopsis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 30
year: '2018'
...
---
_id: '449'
abstract:
- lang: eng
text: Auxin is unique among plant hormones due to its directional transport that
is mediated by the polarly distributed PIN auxin transporters at the plasma membrane.
The canalization hypothesis proposes that the auxin feedback on its polar flow
is a crucial, plant-specific mechanism mediating multiple self-organizing developmental
processes. Here, we used the auxin effect on the PIN polar localization in Arabidopsis
thaliana roots as a proxy for the auxin feedback on the PIN polarity during canalization.
We performed microarray experiments to find regulators of this process that act
downstream of auxin. We identified genes that were transcriptionally regulated
by auxin in an AXR3/IAA17- and ARF7/ARF19-dependent manner. Besides the known
components of the PIN polarity, such as PID and PIP5K kinases, a number of potential
new regulators were detected, among which the WRKY23 transcription factor, which
was characterized in more detail. Gain- and loss-of-function mutants confirmed
a role for WRKY23 in mediating the auxin effect on the PIN polarity. Accordingly,
processes requiring auxin-mediated PIN polarity rearrangements, such as vascular
tissue development during leaf venation, showed a higher WRKY23 expression and
required the WRKY23 activity. Our results provide initial insights into the auxin
transcriptional network acting upstream of PIN polarization and, potentially,
canalization-mediated plant development.
article_processing_charge: Yes
author:
- first_name: Tomas
full_name: Prat, Tomas
id: 3DA3BFEE-F248-11E8-B48F-1D18A9856A87
last_name: Prat
- first_name: Jakub
full_name: Hajny, Jakub
id: 4800CC20-F248-11E8-B48F-1D18A9856A87
last_name: Hajny
orcid: 0000-0003-2140-7195
- first_name: Wim
full_name: Grunewald, Wim
last_name: Grunewald
- first_name: Mina K
full_name: Vasileva, Mina K
id: 3407EB18-F248-11E8-B48F-1D18A9856A87
last_name: Vasileva
- first_name: Gergely
full_name: Molnar, Gergely
id: 34F1AF46-F248-11E8-B48F-1D18A9856A87
last_name: Molnar
- first_name: Ricardo
full_name: Tejos, Ricardo
last_name: Tejos
- first_name: Markus
full_name: Schmid, Markus
last_name: Schmid
- first_name: Michael
full_name: Sauer, Michael
last_name: Sauer
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Prat T, Hajny J, Grunewald W, et al. WRKY23 is a component of the transcriptional
network mediating auxin feedback on PIN polarity. PLoS Genetics. 2018;14(1).
doi:10.1371/journal.pgen.1007177
apa: Prat, T., Hajny, J., Grunewald, W., Vasileva, M. K., Molnar, G., Tejos, R.,
… Friml, J. (2018). WRKY23 is a component of the transcriptional network mediating
auxin feedback on PIN polarity. PLoS Genetics. Public Library of Science.
https://doi.org/10.1371/journal.pgen.1007177
chicago: Prat, Tomas, Jakub Hajny, Wim Grunewald, Mina K Vasileva, Gergely Molnar,
Ricardo Tejos, Markus Schmid, Michael Sauer, and Jiří Friml. “WRKY23 Is a Component
of the Transcriptional Network Mediating Auxin Feedback on PIN Polarity.” PLoS
Genetics. Public Library of Science, 2018. https://doi.org/10.1371/journal.pgen.1007177.
ieee: T. Prat et al., “WRKY23 is a component of the transcriptional network
mediating auxin feedback on PIN polarity,” PLoS Genetics, vol. 14, no.
1. Public Library of Science, 2018.
ista: Prat T, Hajny J, Grunewald W, Vasileva MK, Molnar G, Tejos R, Schmid M, Sauer
M, Friml J. 2018. WRKY23 is a component of the transcriptional network mediating
auxin feedback on PIN polarity. PLoS Genetics. 14(1).
mla: Prat, Tomas, et al. “WRKY23 Is a Component of the Transcriptional Network Mediating
Auxin Feedback on PIN Polarity.” PLoS Genetics, vol. 14, no. 1, Public
Library of Science, 2018, doi:10.1371/journal.pgen.1007177.
short: T. Prat, J. Hajny, W. Grunewald, M.K. Vasileva, G. Molnar, R. Tejos, M. Schmid,
M. Sauer, J. Friml, PLoS Genetics 14 (2018).
date_created: 2018-12-11T11:46:32Z
date_published: 2018-01-29T00:00:00Z
date_updated: 2024-03-28T23:30:38Z
day: '29'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.1371/journal.pgen.1007177
ec_funded: 1
external_id:
isi:
- '000423718600034'
file:
- access_level: open_access
checksum: 0276d66788ec076f4924164a39e6a712
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:10:52Z
date_updated: 2020-07-14T12:46:30Z
file_id: '4843'
file_name: IST-2018-967-v1+1_journal.pgen.1007177.pdf
file_size: 24709062
relation: main_file
file_date_updated: 2020-07-14T12:46:30Z
has_accepted_license: '1'
intvolume: ' 14'
isi: 1
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: PLoS Genetics
publication_status: published
publisher: Public Library of Science
publist_id: '7373'
pubrep_id: '967'
quality_controlled: '1'
related_material:
record:
- id: '1127'
relation: dissertation_contains
status: public
- id: '7172'
relation: dissertation_contains
status: public
- id: '8822'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: WRKY23 is a component of the transcriptional network mediating auxin feedback
on PIN polarity
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 14
year: '2018'
...
---
_id: '191'
abstract:
- lang: eng
text: Intercellular distribution of the plant hormone auxin largely depends on the
polar subcellular distribution of the plasma membrane PIN-FORMED (PIN) auxin transporters.
PIN polarity switches in response to different developmental and environmental
signals have been shown to redirect auxin fluxes mediating certain developmental
responses. PIN phosphorylation at different sites and by different kinases is
crucial for PIN function. Here we investigate the role of PIN phosphorylation
during gravitropic response. Loss- and gain-of-function mutants in PINOID and
related kinases but not in D6PK kinase as well as mutations mimicking constitutive
dephosphorylated or phosphorylated status of two clusters of predicted phosphorylation
sites partially disrupted PIN3 phosphorylation and caused defects in gravitropic
bending in roots and hypocotyls. In particular, they impacted PIN3 polarity rearrangements
in response to gravity and during feed-back regulation by auxin itself. Thus PIN
phosphorylation, besides regulating transport activity and apical-basal targeting,
is also important for the rapid polarity switches in response to environmental
and endogenous signals.
article_number: '10279'
article_processing_charge: No
author:
- first_name: Peter
full_name: Grones, Peter
id: 399876EC-F248-11E8-B48F-1D18A9856A87
last_name: Grones
- first_name: Melinda F
full_name: Abas, Melinda F
id: 3CFB3B1C-F248-11E8-B48F-1D18A9856A87
last_name: Abas
- first_name: Jakub
full_name: Hajny, Jakub
id: 4800CC20-F248-11E8-B48F-1D18A9856A87
last_name: Hajny
orcid: 0000-0003-2140-7195
- first_name: Angharad
full_name: Jones, Angharad
last_name: Jones
- first_name: Sascha
full_name: Waidmann, Sascha
last_name: Waidmann
- first_name: Jürgen
full_name: Kleine Vehn, Jürgen
last_name: Kleine Vehn
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Grones P, Abas MF, Hajny J, et al. PID/WAG-mediated phosphorylation of the
Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism.
Scientific Reports. 2018;8(1). doi:10.1038/s41598-018-28188-1
apa: Grones, P., Abas, M. F., Hajny, J., Jones, A., Waidmann, S., Kleine Vehn, J.,
& Friml, J. (2018). PID/WAG-mediated phosphorylation of the Arabidopsis PIN3
auxin transporter mediates polarity switches during gravitropism. Scientific
Reports. Springer. https://doi.org/10.1038/s41598-018-28188-1
chicago: Grones, Peter, Melinda F Abas, Jakub Hajny, Angharad Jones, Sascha Waidmann,
Jürgen Kleine Vehn, and Jiří Friml. “PID/WAG-Mediated Phosphorylation of the Arabidopsis
PIN3 Auxin Transporter Mediates Polarity Switches during Gravitropism.” Scientific
Reports. Springer, 2018. https://doi.org/10.1038/s41598-018-28188-1.
ieee: P. Grones et al., “PID/WAG-mediated phosphorylation of the Arabidopsis
PIN3 auxin transporter mediates polarity switches during gravitropism,” Scientific
Reports, vol. 8, no. 1. Springer, 2018.
ista: Grones P, Abas MF, Hajny J, Jones A, Waidmann S, Kleine Vehn J, Friml J. 2018.
PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates
polarity switches during gravitropism. Scientific Reports. 8(1), 10279.
mla: Grones, Peter, et al. “PID/WAG-Mediated Phosphorylation of the Arabidopsis
PIN3 Auxin Transporter Mediates Polarity Switches during Gravitropism.” Scientific
Reports, vol. 8, no. 1, 10279, Springer, 2018, doi:10.1038/s41598-018-28188-1.
short: P. Grones, M.F. Abas, J. Hajny, A. Jones, S. Waidmann, J. Kleine Vehn, J.
Friml, Scientific Reports 8 (2018).
date_created: 2018-12-11T11:45:06Z
date_published: 2018-07-06T00:00:00Z
date_updated: 2024-03-28T23:30:38Z
day: '06'
ddc:
- '581'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1038/s41598-018-28188-1
ec_funded: 1
external_id:
isi:
- '000437673200053'
file:
- access_level: open_access
checksum: 266b03f4fb8198e83141617aaa99dcab
content_type: application/pdf
creator: dernst
date_created: 2018-12-17T15:38:56Z
date_updated: 2020-07-14T12:45:20Z
file_id: '5714'
file_name: 2018_ScientificReports_Grones.pdf
file_size: 2413876
relation: main_file
file_date_updated: 2020-07-14T12:45:20Z
has_accepted_license: '1'
intvolume: ' 8'
isi: 1
issue: '1'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Scientific Reports
publication_status: published
publisher: Springer
publist_id: '7729'
quality_controlled: '1'
related_material:
record:
- id: '8822'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter
mediates polarity switches during gravitropism
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 8
year: '2018'
...
---
_id: '442'
abstract:
- lang: eng
text: The rapid auxin-triggered growth of the Arabidopsis hypocotyls involves the
nuclear TIR1/AFB-Aux/IAA signaling and is accompanied by acidification of the
apoplast and cell walls (Fendrych et al., 2016). Here, we describe in detail the
method for analysis of the elongation and the TIR1/AFB-Aux/IAA-dependent auxin
response in hypocotyl segments as well as the determination of relative values
of the cell wall pH.
acknowledgement: 'This protocol was adapted from Fendrych et al., 2016. This project
has received funding from the European Union’s Horizon 2020 research and innovation
programme under the Marie Skłodowska-Curie Grant Agreement No. 665385, and Austrian
Science Fund (FWF) [M 2128-B21]. '
article_processing_charge: No
article_type: original
author:
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Gabriel
full_name: Krens, Gabriel
id: 2B819732-F248-11E8-B48F-1D18A9856A87
last_name: Krens
orcid: 0000-0003-4761-5996
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Li L, Krens G, Fendrych M, Friml J. Real-time analysis of auxin response, cell
wall pH and elongation in Arabidopsis thaliana Hypocotyls. Bio-protocol.
2018;8(1). doi:10.21769/BioProtoc.2685
apa: Li, L., Krens, G., Fendrych, M., & Friml, J. (2018). Real-time analysis
of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls.
Bio-Protocol. Bio-protocol. https://doi.org/10.21769/BioProtoc.2685
chicago: Li, Lanxin, Gabriel Krens, Matyas Fendrych, and Jiří Friml. “Real-Time
Analysis of Auxin Response, Cell Wall PH and Elongation in Arabidopsis Thaliana
Hypocotyls.” Bio-Protocol. Bio-protocol, 2018. https://doi.org/10.21769/BioProtoc.2685.
ieee: L. Li, G. Krens, M. Fendrych, and J. Friml, “Real-time analysis of auxin response,
cell wall pH and elongation in Arabidopsis thaliana Hypocotyls,” Bio-protocol,
vol. 8, no. 1. Bio-protocol, 2018.
ista: Li L, Krens G, Fendrych M, Friml J. 2018. Real-time analysis of auxin response,
cell wall pH and elongation in Arabidopsis thaliana Hypocotyls. Bio-protocol.
8(1).
mla: Li, Lanxin, et al. “Real-Time Analysis of Auxin Response, Cell Wall PH and
Elongation in Arabidopsis Thaliana Hypocotyls.” Bio-Protocol, vol. 8, no.
1, Bio-protocol, 2018, doi:10.21769/BioProtoc.2685.
short: L. Li, G. Krens, M. Fendrych, J. Friml, Bio-Protocol 8 (2018).
date_created: 2018-12-11T11:46:30Z
date_published: 2018-01-05T00:00:00Z
date_updated: 2024-03-28T23:30:43Z
day: '05'
ddc:
- '576'
- '581'
department:
- _id: JiFr
- _id: Bio
doi: 10.21769/BioProtoc.2685
ec_funded: 1
file:
- access_level: open_access
checksum: 6644ba698206eda32b0abf09128e63e3
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:17:43Z
date_updated: 2020-07-14T12:46:29Z
file_id: '5299'
file_name: IST-2018-970-v1+1_2018_Lanxin_Real-time_analysis.pdf
file_size: 11352389
relation: main_file
file_date_updated: 2020-07-14T12:46:29Z
has_accepted_license: '1'
intvolume: ' 8'
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
publication: Bio-protocol
publication_identifier:
eissn:
- 2331-8325
publication_status: published
publisher: Bio-protocol
publist_id: '7381'
pubrep_id: '970'
quality_controlled: '1'
related_material:
record:
- id: '10083'
relation: dissertation_contains
status: public
status: public
title: Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis
thaliana Hypocotyls
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 8
year: '2018'
...
---
_id: '572'
abstract:
- lang: eng
text: In this review, we summarize the different biosynthesis-related pathways that
contribute to the regulation of endogenous auxin in plants. We demonstrate that
all known genes involved in auxin biosynthesis also have a role in root formation,
from the initiation of a root meristem during embryogenesis to the generation
of a functional root system with a primary root, secondary lateral root branches
and adventitious roots. Furthermore, the versatile adaptation of root development
in response to environmental challenges is mediated by both local and distant
control of auxin biosynthesis. In conclusion, auxin homeostasis mediated by spatial
and temporal regulation of auxin biosynthesis plays a central role in determining
root architecture.
article_number: '2587'
article_processing_charge: No
author:
- first_name: Damilola
full_name: Olatunji, Damilola
last_name: Olatunji
- first_name: Danny
full_name: Geelen, Danny
last_name: Geelen
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
citation:
ama: Olatunji D, Geelen D, Verstraeten I. Control of endogenous auxin levels in
plant root development. International Journal of Molecular Sciences. 2017;18(12).
doi:10.3390/ijms18122587
apa: Olatunji, D., Geelen, D., & Verstraeten, I. (2017). Control of endogenous
auxin levels in plant root development. International Journal of Molecular
Sciences. MDPI. https://doi.org/10.3390/ijms18122587
chicago: Olatunji, Damilola, Danny Geelen, and Inge Verstraeten. “Control of Endogenous
Auxin Levels in Plant Root Development.” International Journal of Molecular
Sciences. MDPI, 2017. https://doi.org/10.3390/ijms18122587.
ieee: D. Olatunji, D. Geelen, and I. Verstraeten, “Control of endogenous auxin levels
in plant root development,” International Journal of Molecular Sciences,
vol. 18, no. 12. MDPI, 2017.
ista: Olatunji D, Geelen D, Verstraeten I. 2017. Control of endogenous auxin levels
in plant root development. International Journal of Molecular Sciences. 18(12),
2587.
mla: Olatunji, Damilola, et al. “Control of Endogenous Auxin Levels in Plant Root
Development.” International Journal of Molecular Sciences, vol. 18, no.
12, 2587, MDPI, 2017, doi:10.3390/ijms18122587.
short: D. Olatunji, D. Geelen, I. Verstraeten, International Journal of Molecular
Sciences 18 (2017).
date_created: 2018-12-11T11:47:15Z
date_published: 2017-12-01T00:00:00Z
date_updated: 2021-01-12T08:03:16Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.3390/ijms18122587
file:
- access_level: open_access
checksum: 82d51f11e493f7eec02976d9a9a9805e
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:08:55Z
date_updated: 2020-07-14T12:47:10Z
file_id: '4718'
file_name: IST-2017-917-v1+1_ijms-18-02587.pdf
file_size: 920962
relation: main_file
file_date_updated: 2020-07-14T12:47:10Z
has_accepted_license: '1'
intvolume: ' 18'
issue: '12'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
publication: International Journal of Molecular Sciences
publication_status: published
publisher: MDPI
publist_id: '7242'
pubrep_id: '917'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Control of endogenous auxin levels in plant root development
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 18
year: '2017'
...
---
_id: '657'
abstract:
- lang: eng
text: Plant organs are typically organized into three main tissue layers. The middle
ground tissue layer comprises the majority of the plant body and serves a wide
range of functions, including photosynthesis, selective nutrient uptake and storage,
and gravity sensing. Ground tissue patterning and maintenance in Arabidopsis are
controlled by a well-established gene network revolving around the key regulator
SHORT-ROOT (SHR). In contrast, it is completely unknown how ground tissue identity
is first specified from totipotent precursor cells in the embryo. The plant signaling
molecule auxin, acting through AUXIN RESPONSE FACTOR (ARF) transcription factors,
is critical for embryo patterning. The auxin effector ARF5/MONOPTEROS (MP) acts
both cell-autonomously and noncell-autonomously to control embryonic vascular
tissue formation and root initiation, respectively. Here we show that auxin response
and ARF activity cell-autonomously control the asymmetric division of the first
ground tissue cells. By identifying embryonic target genes, we show that MP transcriptionally
initiates the ground tissue lineage and acts upstream of the regulatory network
that controls ground tissue patterning and maintenance. Strikingly, whereas the
SHR network depends on MP, this MP function is, at least in part, SHR independent.
Our study therefore identifies auxin response as a regulator of ground tissue
specification in the embryonic root, and reveals that ground tissue initiation
and maintenance use different regulators and mechanisms. Moreover, our data provide
a framework for the simultaneous formation of multiple cell types by the same
transcriptional regulator.
author:
- first_name: Barbara
full_name: Möller, Barbara
last_name: Möller
- first_name: Colette
full_name: Ten Hove, Colette
last_name: Ten Hove
- first_name: Daoquan
full_name: Xiang, Daoquan
last_name: Xiang
- first_name: Nerys
full_name: Williams, Nerys
last_name: Williams
- first_name: Lorena
full_name: López, Lorena
last_name: López
- first_name: Saiko
full_name: Yoshida, Saiko
id: 2E46069C-F248-11E8-B48F-1D18A9856A87
last_name: Yoshida
- first_name: Margot
full_name: Smit, Margot
last_name: Smit
- first_name: Raju
full_name: Datla, Raju
last_name: Datla
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
citation:
ama: Möller B, Ten Hove C, Xiang D, et al. Auxin response cell autonomously controls
ground tissue initiation in the early arabidopsis embryo. PNAS. 2017;114(12):E2533-E2539.
doi:10.1073/pnas.1616493114
apa: Möller, B., Ten Hove, C., Xiang, D., Williams, N., López, L., Yoshida, S.,
… Weijers, D. (2017). Auxin response cell autonomously controls ground tissue
initiation in the early arabidopsis embryo. PNAS. National Academy of Sciences.
https://doi.org/10.1073/pnas.1616493114
chicago: Möller, Barbara, Colette Ten Hove, Daoquan Xiang, Nerys Williams, Lorena
López, Saiko Yoshida, Margot Smit, Raju Datla, and Dolf Weijers. “Auxin Response
Cell Autonomously Controls Ground Tissue Initiation in the Early Arabidopsis Embryo.”
PNAS. National Academy of Sciences, 2017. https://doi.org/10.1073/pnas.1616493114.
ieee: B. Möller et al., “Auxin response cell autonomously controls ground
tissue initiation in the early arabidopsis embryo,” PNAS, vol. 114, no.
12. National Academy of Sciences, pp. E2533–E2539, 2017.
ista: Möller B, Ten Hove C, Xiang D, Williams N, López L, Yoshida S, Smit M, Datla
R, Weijers D. 2017. Auxin response cell autonomously controls ground tissue initiation
in the early arabidopsis embryo. PNAS. 114(12), E2533–E2539.
mla: Möller, Barbara, et al. “Auxin Response Cell Autonomously Controls Ground Tissue
Initiation in the Early Arabidopsis Embryo.” PNAS, vol. 114, no. 12, National
Academy of Sciences, 2017, pp. E2533–39, doi:10.1073/pnas.1616493114.
short: B. Möller, C. Ten Hove, D. Xiang, N. Williams, L. López, S. Yoshida, M. Smit,
R. Datla, D. Weijers, PNAS 114 (2017) E2533–E2539.
date_created: 2018-12-11T11:47:45Z
date_published: 2017-03-21T00:00:00Z
date_updated: 2021-01-12T08:08:02Z
day: '21'
department:
- _id: JiFr
doi: 10.1073/pnas.1616493114
external_id:
pmid:
- '28265057'
intvolume: ' 114'
issue: '12'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5373392/
month: '03'
oa: 1
oa_version: Submitted Version
page: E2533 - E2539
pmid: 1
publication: PNAS
publication_identifier:
issn:
- '00278424'
publication_status: published
publisher: National Academy of Sciences
publist_id: '7076'
quality_controlled: '1'
scopus_import: 1
status: public
title: Auxin response cell autonomously controls ground tissue initiation in the early
arabidopsis embryo
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 114
year: '2017'
...
---
_id: '669'
abstract:
- lang: eng
text: 'The exocyst, a eukaryotic tethering complex, coregulates targeted exocytosis
as an effector of small GTPases in polarized cell growth. In land plants, several
exocyst subunits are encoded by double or triple paralogs, culminating in tens
of EXO70 paralogs. Out of 23 Arabidopsis thaliana EXO70 isoforms, we analyzed
seven isoforms expressed in pollen. Genetic and microscopic analyses of single
mutants in EXO70A2, EXO70C1, EXO70C2, EXO70F1, EXO70H3, EXO70H5, and EXO70H6 genes
revealed that only a loss-of-function EXO70C2 allele resulted in a significant
male-specific transmission defect (segregation 40%:51%:9%) due to aberrant pollen
tube growth. Mutant pollen tubes grown in vitro exhibited an enhanced growth rate
and a decreased thickness of the tip cell wall, causing tip bursts. However, exo70C2
pollen tubes could frequently recover and restart their speedy elongation, resulting
in a repetitive stop-and-go growth dynamics. A pollenspecific depletion of the
closest paralog, EXO70C1, using artificial microRNA in the exo70C2 mutant background,
resulted in a complete pollen-specific transmission defect, suggesting redundant
functions of EXO70C1 and EXO70C2. Both EXO70C1 and EXO70C2, GFP tagged and expressed
under the control of their native promoters, localized in the cytoplasm of pollen
grains, pollen tubes, and also root trichoblast cells. The expression of EXO70C2-GFP
complemented the aberrant growth of exo70C2 pollen tubes. The absent EXO70C2 interactions
with core exocyst subunits in the yeast two-hybrid assay, cytoplasmic localization,
and genetic effect suggest an unconventional EXO70 function possibly as a regulator
of exocytosis outside the exocyst complex. In conclusion, EXO70C2 is a novel factor
contributing to the regulation of optimal tip growth of Arabidopsis pollen tubes. '
article_processing_charge: No
article_type: original
author:
- first_name: Lukáš
full_name: Synek, Lukáš
last_name: Synek
- first_name: Nemanja
full_name: Vukašinović, Nemanja
last_name: Vukašinović
- first_name: Ivan
full_name: Kulich, Ivan
last_name: Kulich
- first_name: Michal
full_name: Hála, Michal
last_name: Hála
- first_name: Klára
full_name: Aldorfová, Klára
last_name: Aldorfová
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: Viktor
full_name: Žárský, Viktor
last_name: Žárský
citation:
ama: Synek L, Vukašinović N, Kulich I, et al. EXO70C2 is a key regulatory factor
for optimal tip growth of pollen. Plant Physiology. 2017;174(1):223-240.
doi:10.1104/pp.16.01282
apa: Synek, L., Vukašinović, N., Kulich, I., Hála, M., Aldorfová, K., Fendrych,
M., & Žárský, V. (2017). EXO70C2 is a key regulatory factor for optimal tip
growth of pollen. Plant Physiology. American Society of Plant Biologists.
https://doi.org/10.1104/pp.16.01282
chicago: Synek, Lukáš, Nemanja Vukašinović, Ivan Kulich, Michal Hála, Klára Aldorfová,
Matyas Fendrych, and Viktor Žárský. “EXO70C2 Is a Key Regulatory Factor for Optimal
Tip Growth of Pollen.” Plant Physiology. American Society of Plant Biologists,
2017. https://doi.org/10.1104/pp.16.01282.
ieee: L. Synek et al., “EXO70C2 is a key regulatory factor for optimal tip
growth of pollen,” Plant Physiology, vol. 174, no. 1. American Society
of Plant Biologists, pp. 223–240, 2017.
ista: Synek L, Vukašinović N, Kulich I, Hála M, Aldorfová K, Fendrych M, Žárský
V. 2017. EXO70C2 is a key regulatory factor for optimal tip growth of pollen.
Plant Physiology. 174(1), 223–240.
mla: Synek, Lukáš, et al. “EXO70C2 Is a Key Regulatory Factor for Optimal Tip Growth
of Pollen.” Plant Physiology, vol. 174, no. 1, American Society of Plant
Biologists, 2017, pp. 223–40, doi:10.1104/pp.16.01282.
short: L. Synek, N. Vukašinović, I. Kulich, M. Hála, K. Aldorfová, M. Fendrych,
V. Žárský, Plant Physiology 174 (2017) 223–240.
date_created: 2018-12-11T11:47:49Z
date_published: 2017-05-01T00:00:00Z
date_updated: 2021-01-12T08:08:35Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1104/pp.16.01282
external_id:
pmid:
- '28356503'
file:
- access_level: open_access
checksum: 97155acc6aa5f0d0a78e0589a932fe02
content_type: application/pdf
creator: dernst
date_created: 2019-11-18T16:16:18Z
date_updated: 2020-07-14T12:47:37Z
file_id: '7041'
file_name: 2017_PlantPhysio_Synek.pdf
file_size: 2176903
relation: main_file
file_date_updated: 2020-07-14T12:47:37Z
has_accepted_license: '1'
intvolume: ' 174'
issue: '1'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Submitted Version
page: 223 - 240
pmid: 1
publication: Plant Physiology
publication_identifier:
issn:
- '00320889'
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '7058'
quality_controlled: '1'
scopus_import: 1
status: public
title: EXO70C2 is a key regulatory factor for optimal tip growth of pollen
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 174
year: '2017'
...
---
_id: '722'
abstract:
- lang: eng
text: Plants are sessile organisms rooted in one place. The soil resources that
plants require are often distributed in a highly heterogeneous pattern. To aid
foraging, plants have evolved roots whose growth and development are highly responsive
to soil signals. As a result, 3D root architecture is shaped by myriad environmental
signals to ensure resource capture is optimised and unfavourable environments
are avoided. The first signals sensed by newly germinating seeds — gravity and
light — direct root growth into the soil to aid seedling establishment. Heterogeneous
soil resources, such as water, nitrogen and phosphate, also act as signals that
shape 3D root growth to optimise uptake. Root architecture is also modified through
biotic interactions that include soil fungi and neighbouring plants. This developmental
plasticity results in a ‘custom-made’ 3D root system that is best adapted to forage
for resources in each soil environment that a plant colonises.
author:
- first_name: Emily
full_name: Morris, Emily
last_name: Morris
- first_name: Marcus
full_name: Griffiths, Marcus
last_name: Griffiths
- first_name: Agata
full_name: Golebiowska, Agata
last_name: Golebiowska
- first_name: Stefan
full_name: Mairhofer, Stefan
last_name: Mairhofer
- first_name: Jasmine
full_name: Burr Hersey, Jasmine
last_name: Burr Hersey
- first_name: Tatsuaki
full_name: Goh, Tatsuaki
last_name: Goh
- first_name: Daniel
full_name: Von Wangenheim, Daniel
id: 49E91952-F248-11E8-B48F-1D18A9856A87
last_name: Von Wangenheim
orcid: 0000-0002-6862-1247
- first_name: Brian
full_name: Atkinson, Brian
last_name: Atkinson
- first_name: Craig
full_name: Sturrock, Craig
last_name: Sturrock
- first_name: Jonathan
full_name: Lynch, Jonathan
last_name: Lynch
- first_name: Kris
full_name: Vissenberg, Kris
last_name: Vissenberg
- first_name: Karl
full_name: Ritz, Karl
last_name: Ritz
- first_name: Darren
full_name: Wells, Darren
last_name: Wells
- first_name: Sacha
full_name: Mooney, Sacha
last_name: Mooney
- first_name: Malcolm
full_name: Bennett, Malcolm
last_name: Bennett
citation:
ama: Morris E, Griffiths M, Golebiowska A, et al. Shaping 3D root system architecture.
Current Biology. 2017;27(17):R919-R930. doi:10.1016/j.cub.2017.06.043
apa: Morris, E., Griffiths, M., Golebiowska, A., Mairhofer, S., Burr Hersey, J.,
Goh, T., … Bennett, M. (2017). Shaping 3D root system architecture. Current
Biology. Cell Press. https://doi.org/10.1016/j.cub.2017.06.043
chicago: Morris, Emily, Marcus Griffiths, Agata Golebiowska, Stefan Mairhofer, Jasmine
Burr Hersey, Tatsuaki Goh, Daniel von Wangenheim, et al. “Shaping 3D Root System
Architecture.” Current Biology. Cell Press, 2017. https://doi.org/10.1016/j.cub.2017.06.043.
ieee: E. Morris et al., “Shaping 3D root system architecture,” Current
Biology, vol. 27, no. 17. Cell Press, pp. R919–R930, 2017.
ista: Morris E, Griffiths M, Golebiowska A, Mairhofer S, Burr Hersey J, Goh T, von
Wangenheim D, Atkinson B, Sturrock C, Lynch J, Vissenberg K, Ritz K, Wells D,
Mooney S, Bennett M. 2017. Shaping 3D root system architecture. Current Biology.
27(17), R919–R930.
mla: Morris, Emily, et al. “Shaping 3D Root System Architecture.” Current Biology,
vol. 27, no. 17, Cell Press, 2017, pp. R919–30, doi:10.1016/j.cub.2017.06.043.
short: E. Morris, M. Griffiths, A. Golebiowska, S. Mairhofer, J. Burr Hersey, T.
Goh, D. von Wangenheim, B. Atkinson, C. Sturrock, J. Lynch, K. Vissenberg, K.
Ritz, D. Wells, S. Mooney, M. Bennett, Current Biology 27 (2017) R919–R930.
date_created: 2018-12-11T11:48:08Z
date_published: 2017-09-11T00:00:00Z
date_updated: 2021-01-12T08:12:29Z
day: '11'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.1016/j.cub.2017.06.043
ec_funded: 1
external_id:
pmid:
- '28898665'
file:
- access_level: open_access
checksum: e45588b21097b408da6276a3e5eedb2e
content_type: application/pdf
creator: dernst
date_created: 2019-04-17T07:46:40Z
date_updated: 2020-07-14T12:47:54Z
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file_name: 2017_CurrentBiology_Morris.pdf
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has_accepted_license: '1'
intvolume: ' 27'
issue: '17'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Submitted Version
page: R919 - R930
pmid: 1
project:
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
publication: Current Biology
publication_identifier:
issn:
- '09609822'
publication_status: published
publisher: Cell Press
publist_id: '6956'
pubrep_id: '982'
quality_controlled: '1'
scopus_import: 1
status: public
title: Shaping 3D root system architecture
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 27
year: '2017'
...
---
_id: '938'
abstract:
- lang: eng
text: The thesis encompasses several topics of plant cell biology which were studied
in the model plant Arabidopsis thaliana. Chapter 1 concerns the plant hormone
auxin and its polar transport through cells and tissues. The highly controlled,
directional transport of auxin is facilitated by plasma membrane-localized transporters.
Transporters from the PIN family direct auxin transport due to their polarized
localizations at cell membranes. Substantial effort has been put into research
on cellular trafficking of PIN proteins, which is thought to underlie their polar
distribution. I participated in a forward genetic screen aimed at identifying
novel regulators of PIN polarity. The screen yielded several genes which may be
involved in PIN polarity regulation or participate in polar auxin transport by
other means. Chapter 2 focuses on the endomembrane system, with particular attention
to clathrin-mediated endocytosis. The project started with identification of several
proteins that interact with clathrin light chains. Among them, I focused on two
putative homologues of auxilin, which in non-plant systems is an endocytotic factor
known for uncoating clathrin-coated vesicles in the final step of endocytosis.
The body of my work consisted of an in-depth characterization of transgenic A.
thaliana lines overexpressing these putative auxilins in an inducible manner.
Overexpression of these proteins leads to an inhibition of endocytosis, as documented
by imaging of cargoes and clathrin-related endocytic machinery. An extension of
this work is an investigation into a concept of homeostatic regulation acting
between distinct transport processes in the endomembrane system. With auxilin
overexpressing lines, where endocytosis is blocked specifically, I made observations
on the mutual relationship between two opposite trafficking processes of secretion
and endocytosis. In Chapter 3, I analyze cortical microtubule arrays and their
relationship to auxin signaling and polarized growth in elongating cells. In plants,
microtubules are organized into arrays just below the plasma membrane, and it
is thought that their function is to guide membrane-docked cellulose synthase
complexes. These, in turn, influence cell wall structure and cell shape by directed
deposition of cellulose fibres. In elongating cells, cortical microtubule arrays
are able to reorient in relation to long cell axis, and these reorientations have
been linked to cell growth and to signaling of growth-regulating factors such
as auxin or light. In this chapter, I am addressing the causal relationship between
microtubule array reorientation, growth, and auxin signaling. I arrive at a model
where array reorientation is not guided by auxin directly, but instead is only
controlled by growth, which, in turn, is regulated by auxin.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
citation:
ama: Adamowski M. Investigations into cell polarity and trafficking in the plant
model Arabidopsis thaliana . 2017. doi:10.15479/AT:ISTA:th_842
apa: Adamowski, M. (2017). Investigations into cell polarity and trafficking
in the plant model Arabidopsis thaliana . Institute of Science and Technology
Austria. https://doi.org/10.15479/AT:ISTA:th_842
chicago: Adamowski, Maciek. “Investigations into Cell Polarity and Trafficking in
the Plant Model Arabidopsis Thaliana .” Institute of Science and Technology Austria,
2017. https://doi.org/10.15479/AT:ISTA:th_842.
ieee: M. Adamowski, “Investigations into cell polarity and trafficking in the plant
model Arabidopsis thaliana ,” Institute of Science and Technology Austria, 2017.
ista: Adamowski M. 2017. Investigations into cell polarity and trafficking in the
plant model Arabidopsis thaliana . Institute of Science and Technology Austria.
mla: Adamowski, Maciek. Investigations into Cell Polarity and Trafficking in
the Plant Model Arabidopsis Thaliana . Institute of Science and Technology
Austria, 2017, doi:10.15479/AT:ISTA:th_842.
short: M. Adamowski, Investigations into Cell Polarity and Trafficking in the Plant
Model Arabidopsis Thaliana , Institute of Science and Technology Austria, 2017.
date_created: 2018-12-11T11:49:18Z
date_published: 2017-06-02T00:00:00Z
date_updated: 2023-09-07T12:06:09Z
day: '02'
ddc:
- '581'
- '583'
- '580'
degree_awarded: PhD
department:
- _id: JiFr
doi: 10.15479/AT:ISTA:th_842
file:
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language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
page: '117'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '6483'
pubrep_id: '842'
related_material:
record:
- id: '1591'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
title: 'Investigations into cell polarity and trafficking in the plant model Arabidopsis
thaliana '
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2017'
...
---
_id: '1127'
abstract:
- lang: eng
text: "Plant hormone auxin and its transport between cells belong to the most important\r\nmechanisms
controlling plant development. Auxin itself could change localization of PINs
and\r\nthereby control direction of its own flow. We performed an expression profiling
experiment\r\nin Arabidopsis roots to identify potential regulators of PIN polarity
which are transcriptionally\r\nregulated by auxin signalling. We identified several
novel regulators and performed a detailed\r\ncharacterization of the transcription
factor WRKY23 (At2g47260) and its role in auxin\r\nfeedback on PIN polarity. Gain-of-function
and dominant-negative mutants revealed that\r\nWRKY23 plays a crucial role in
mediating the auxin effect on PIN polarity. In concordance,\r\ntypical polar auxin
transport processes such as gravitropism and leaf vascular pattern\r\nformation
were disturbed by interfering with WRKY23 function.\r\nIn order to identify direct
targets of WRKY23, we performed consequential expression\r\nprofiling experiments
using a WRKY23 inducible gain-of-function line and dominant-negative\r\nWRKY23
line that is defunct in PIN re-arrangement. Among several genes mostly related
to\r\nthe groups of cell wall and defense process regulators, we identified LYSINE-HISTIDINE\r\nTRANSPORTER
1 (LHT1; At5g40780), a small amino acid permease gene from the amino\r\nacid/auxin
permease family (AAAP), we present its detailed characterisation in auxin feedback\r\non
PIN repolarization, identified its transcriptional regulation, we propose a potential\r\nmechanism
of its action. Moreover, we identified also a member of receptor-like protein\r\nkinase
LRR-RLK (LEUCINE-RICH REPEAT TRANSMEMBRANE PROTEIN KINASE PROTEIN 1;\r\nLRRK1;
At1g05700), which also affects auxin-dependent PIN re-arrangement. We described\r\nits
transcriptional behaviour, subcellular localization. Based on global expression
data, we\r\ntried to identify ligand responsible for mechanism of signalling and
suggest signalling partner\r\nand interactors. Additionally, we described role
of novel phytohormone group, strigolactone,\r\nin auxin-dependent PIN re-arrangement,
that could be a fundament for future studies in this\r\nfield.\r\nOur results
provide first insights into an auxin transcriptional network targeting PIN\r\nlocalization
and thus regulating plant development. We highlighted WRKY23 transcriptional\r\nnetwork
and characterised its mediatory role in plant development. We identified direct\r\neffectors
of this network, LHT1 and LRRK1, and describe their roles in PIN re-arrangement
and\r\nPIN-dependent auxin transport processes."
acknowledgement: I would like to first acknowledge my supervisor Jiří Friml for support,
kind advice and patience. It was a pleasure to be a part of your lab, Jiří. I will
remember the atmosphere present in auxin lab at VIB in Ghent and at IST in Klosterneuburg
forever. I would like to thank all past and present lab members for the friendship
and friendly and scientific environment in the groups. It was so nice to cooperate
with you, guys. There was always someone who helped me with experiments, troubleshoot
issues coming from our work etc. At this place, I would like to thank especially
to Gergo Molnár. I’m happy (and lucky) that I have met him; he naturally became
my tutor and guide through my PhD. From no one else during my entire professional
career, I’ve learned that much.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Tomas
full_name: Prat, Tomas
id: 3DA3BFEE-F248-11E8-B48F-1D18A9856A87
last_name: Prat
citation:
ama: Prat T. Identification of novel regulators of PIN polarity and development
of novel auxin sensor. 2017.
apa: Prat, T. (2017). Identification of novel regulators of PIN polarity and
development of novel auxin sensor. Institute of Science and Technology Austria.
chicago: Prat, Tomas. “Identification of Novel Regulators of PIN Polarity and Development
of Novel Auxin Sensor.” Institute of Science and Technology Austria, 2017.
ieee: T. Prat, “Identification of novel regulators of PIN polarity and development
of novel auxin sensor,” Institute of Science and Technology Austria, 2017.
ista: Prat T. 2017. Identification of novel regulators of PIN polarity and development
of novel auxin sensor. Institute of Science and Technology Austria.
mla: Prat, Tomas. Identification of Novel Regulators of PIN Polarity and Development
of Novel Auxin Sensor. Institute of Science and Technology Austria, 2017.
short: T. Prat, Identification of Novel Regulators of PIN Polarity and Development
of Novel Auxin Sensor, Institute of Science and Technology Austria, 2017.
date_created: 2018-12-11T11:50:17Z
date_published: 2017-01-12T00:00:00Z
date_updated: 2023-09-19T10:39:33Z
day: '12'
ddc:
- '580'
degree_awarded: PhD
department:
- _id: JiFr
file:
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checksum: d192c7c6c5ea32c8432437286dc4909e
content_type: application/pdf
creator: dernst
date_created: 2019-04-05T08:45:14Z
date_updated: 2019-04-05T08:45:14Z
file_id: '6209'
file_name: IST_Austria_Thesis_Tomáš_Prát.pdf
file_size: 10285946
relation: main_file
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checksum: bab18b52cf98145926042d8ed99fdb3b
content_type: application/pdf
creator: dernst
date_created: 2021-02-22T11:52:56Z
date_updated: 2021-02-22T11:52:56Z
file_id: '9185'
file_name: 2017_Thesis_Prat.pdf
file_size: 9802991
relation: main_file
success: 1
file_date_updated: 2021-02-22T11:52:56Z
has_accepted_license: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: '131'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '6233'
related_material:
record:
- id: '449'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
title: Identification of novel regulators of PIN polarity and development of novel
auxin sensor
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2017'
...
---
_id: '1159'
abstract:
- lang: eng
text: Auxin steers numerous physiological processes in plants, making the tight
control of its endogenous levels and spatiotemporal distribution a necessity.
This regulation is achieved by different mechanisms, including auxin biosynthesis,
metabolic conversions, degradation, and transport. Here, we introduce cis-cinnamic
acid (c-CA) as a novel and unique addition to a small group of endogenous molecules
affecting in planta auxin concentrations. c-CA is the photo-isomerization product
of the phenylpropanoid pathway intermediate trans-CA (t-CA). When grown on c-CA-containing
medium, an evolutionary diverse set of plant species were shown to exhibit phenotypes
characteristic for high auxin levels, including inhibition of primary root growth,
induction of root hairs, and promotion of adventitious and lateral rooting. By
molecular docking and receptor binding assays, we showed that c-CA itself is neither
an auxin nor an anti-auxin, and auxin profiling data revealed that c-CA does not
significantly interfere with auxin biosynthesis. Single cell-based auxin accumulation
assays showed that c-CA, and not t-CA, is a potent inhibitor of auxin efflux.
Auxin signaling reporters detected changes in spatiotemporal distribution of the
auxin response along the root of c-CA-treated plants, and long-distance auxin
transport assays showed no inhibition of rootward auxin transport. Overall, these
results suggest that the phenotypes of c-CA-treated plants are the consequence
of a local change in auxin accumulation, induced by the inhibition of auxin efflux.
This work reveals a novel mechanism how plants may regulate auxin levels and adds
a novel, naturally occurring molecule to the chemical toolbox for the studies
of auxin homeostasis.
article_processing_charge: No
article_type: original
author:
- first_name: Ward
full_name: Steenackers, Ward
last_name: Steenackers
- first_name: Petr
full_name: Klíma, Petr
last_name: Klíma
- first_name: Mussa
full_name: Quareshy, Mussa
last_name: Quareshy
- first_name: Igor
full_name: Cesarino, Igor
last_name: Cesarino
- first_name: Robert
full_name: Kumpf, Robert
last_name: Kumpf
- first_name: Sander
full_name: Corneillie, Sander
last_name: Corneillie
- first_name: Pedro
full_name: Araújo, Pedro
last_name: Araújo
- first_name: Tom
full_name: Viaene, Tom
last_name: Viaene
- first_name: Geert
full_name: Goeminne, Geert
last_name: Goeminne
- first_name: Moritz
full_name: Nowack, Moritz
last_name: Nowack
- first_name: Karin
full_name: Ljung, Karin
last_name: Ljung
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Joshua
full_name: Blakeslee, Joshua
last_name: Blakeslee
- first_name: Ondřej
full_name: Novák, Ondřej
last_name: Novák
- first_name: Eva
full_name: Zažímalová, Eva
last_name: Zažímalová
- first_name: Richard
full_name: Napier, Richard
last_name: Napier
- first_name: Wout
full_name: Boerjan, Wout
last_name: Boerjan
- first_name: Bartel
full_name: Vanholme, Bartel
last_name: Vanholme
citation:
ama: Steenackers W, Klíma P, Quareshy M, et al. Cis-cinnamic acid is a novel natural
auxin efflux inhibitor that promotes lateral root formation. Plant Physiology.
2017;173(1):552-565. doi:10.1104/pp.16.00943
apa: Steenackers, W., Klíma, P., Quareshy, M., Cesarino, I., Kumpf, R., Corneillie,
S., … Vanholme, B. (2017). Cis-cinnamic acid is a novel natural auxin efflux inhibitor
that promotes lateral root formation. Plant Physiology. American Society
of Plant Biologists. https://doi.org/10.1104/pp.16.00943
chicago: Steenackers, Ward, Petr Klíma, Mussa Quareshy, Igor Cesarino, Robert Kumpf,
Sander Corneillie, Pedro Araújo, et al. “Cis-Cinnamic Acid Is a Novel Natural
Auxin Efflux Inhibitor That Promotes Lateral Root Formation.” Plant Physiology.
American Society of Plant Biologists, 2017. https://doi.org/10.1104/pp.16.00943.
ieee: W. Steenackers et al., “Cis-cinnamic acid is a novel natural auxin
efflux inhibitor that promotes lateral root formation,” Plant Physiology,
vol. 173, no. 1. American Society of Plant Biologists, pp. 552–565, 2017.
ista: Steenackers W, Klíma P, Quareshy M, Cesarino I, Kumpf R, Corneillie S, Araújo
P, Viaene T, Goeminne G, Nowack M, Ljung K, Friml J, Blakeslee J, Novák O, Zažímalová
E, Napier R, Boerjan W, Vanholme B. 2017. Cis-cinnamic acid is a novel natural
auxin efflux inhibitor that promotes lateral root formation. Plant Physiology.
173(1), 552–565.
mla: Steenackers, Ward, et al. “Cis-Cinnamic Acid Is a Novel Natural Auxin Efflux
Inhibitor That Promotes Lateral Root Formation.” Plant Physiology, vol.
173, no. 1, American Society of Plant Biologists, 2017, pp. 552–65, doi:10.1104/pp.16.00943.
short: W. Steenackers, P. Klíma, M. Quareshy, I. Cesarino, R. Kumpf, S. Corneillie,
P. Araújo, T. Viaene, G. Goeminne, M. Nowack, K. Ljung, J. Friml, J. Blakeslee,
O. Novák, E. Zažímalová, R. Napier, W. Boerjan, B. Vanholme, Plant Physiology
173 (2017) 552–565.
date_created: 2018-12-11T11:50:28Z
date_published: 2017-01-01T00:00:00Z
date_updated: 2023-09-20T11:29:17Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1104/pp.16.00943
ec_funded: 1
external_id:
isi:
- '000394135800041'
pmid:
- '27837086'
file:
- access_level: open_access
checksum: fd4d1cfe7ed70e54bb12ae3881f3fb91
content_type: application/pdf
creator: dernst
date_created: 2019-11-18T16:12:25Z
date_updated: 2020-07-14T12:44:36Z
file_id: '7040'
file_name: 2016_PlantPhysi_Steenackers.pdf
file_size: 4109142
relation: main_file
file_date_updated: 2020-07-14T12:44:36Z
has_accepted_license: '1'
intvolume: ' 173'
isi: 1
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Submitted Version
page: 552 - 565
pmid: 1
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Plant Physiology
publication_identifier:
issn:
- 0032-0889
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '6199'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Cis-cinnamic acid is a novel natural auxin efflux inhibitor that promotes lateral
root formation
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 173
year: '2017'
...
---
_id: '1110'
abstract:
- lang: eng
text: The phytohormone auxin is a major determinant and regulatory component important
for plant development. Auxin transport between cells is mediated by a complex
system of transporters such as AUX1/LAX, PIN, and ABCB proteins, and their localization
and activity is thought to be influenced by phosphatases and kinases. Flavonols
have been shown to alter auxin transport activity and changes in flavonol accumulation
in the Arabidopsis thaliana rol1-2 mutant cause defects in auxin transport and
seedling development. A new mutation in ROOTS CURL IN NPA 1 (RCN1), encoding a
regulatory subunit of the phosphatase PP2A, was found to suppress the growth defects
of rol1-2 without changing the flavonol content. rol1-2 rcn1-3 double mutants
show wild type-like auxin transport activity while levels of free auxin are not
affected by rcn1-3. In the rol1-2 mutant, PIN2 shows a flavonol-induced basal-to-apical
shift in polar localization which is reversed in the rol1-2 rcn1-3 to basal localization.
In vivo analysis of PINOID action, a kinase known to influence PIN protein localization
in a PP2A-antagonistic manner, revealed a negative impact of flavonols on PINOID
activity. Together, these data suggest that flavonols affect auxin transport by
modifying the antagonistic kinase/phosphatase equilibrium.
acknowledgement: European Research Council (project ERC-2011-StG-20101109-PSDP), European
Social Fund (CZ.1.07/2.3.00/20.0043) and the Czech Science Foundation (GA13-40637S)
[JF].
article_number: '41906'
article_processing_charge: No
author:
- first_name: Benjamin
full_name: Kuhn, Benjamin
last_name: Kuhn
- first_name: Tomasz
full_name: Nodzyński, Tomasz
last_name: Nodzyński
- first_name: Sanae
full_name: Errafi, Sanae
last_name: Errafi
- first_name: Rahel
full_name: Bucher, Rahel
last_name: Bucher
- first_name: Shibu
full_name: Gupta, Shibu
last_name: Gupta
- first_name: Bibek
full_name: Aryal, Bibek
last_name: Aryal
- first_name: Petre
full_name: Dobrev, Petre
last_name: Dobrev
- first_name: Laurent
full_name: Bigler, Laurent
last_name: Bigler
- first_name: Markus
full_name: Geisler, Markus
last_name: Geisler
- first_name: Eva
full_name: Zažímalová, Eva
last_name: Zažímalová
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Christoph
full_name: Ringli, Christoph
last_name: Ringli
citation:
ama: Kuhn B, Nodzyński T, Errafi S, et al. Flavonol-induced changes in PIN2 polarity
and auxin transport in the Arabidopsis thaliana rol1-2 mutant require phosphatase
activity. Scientific Reports. 2017;7. doi:10.1038/srep41906
apa: Kuhn, B., Nodzyński, T., Errafi, S., Bucher, R., Gupta, S., Aryal, B., … Ringli,
C. (2017). Flavonol-induced changes in PIN2 polarity and auxin transport in the
Arabidopsis thaliana rol1-2 mutant require phosphatase activity. Scientific
Reports. Nature Publishing Group. https://doi.org/10.1038/srep41906
chicago: Kuhn, Benjamin, Tomasz Nodzyński, Sanae Errafi, Rahel Bucher, Shibu Gupta,
Bibek Aryal, Petre Dobrev, et al. “Flavonol-Induced Changes in PIN2 Polarity and
Auxin Transport in the Arabidopsis Thaliana Rol1-2 Mutant Require Phosphatase
Activity.” Scientific Reports. Nature Publishing Group, 2017. https://doi.org/10.1038/srep41906.
ieee: B. Kuhn et al., “Flavonol-induced changes in PIN2 polarity and auxin
transport in the Arabidopsis thaliana rol1-2 mutant require phosphatase activity,”
Scientific Reports, vol. 7. Nature Publishing Group, 2017.
ista: Kuhn B, Nodzyński T, Errafi S, Bucher R, Gupta S, Aryal B, Dobrev P, Bigler
L, Geisler M, Zažímalová E, Friml J, Ringli C. 2017. Flavonol-induced changes
in PIN2 polarity and auxin transport in the Arabidopsis thaliana rol1-2 mutant
require phosphatase activity. Scientific Reports. 7, 41906.
mla: Kuhn, Benjamin, et al. “Flavonol-Induced Changes in PIN2 Polarity and Auxin
Transport in the Arabidopsis Thaliana Rol1-2 Mutant Require Phosphatase Activity.”
Scientific Reports, vol. 7, 41906, Nature Publishing Group, 2017, doi:10.1038/srep41906.
short: B. Kuhn, T. Nodzyński, S. Errafi, R. Bucher, S. Gupta, B. Aryal, P. Dobrev,
L. Bigler, M. Geisler, E. Zažímalová, J. Friml, C. Ringli, Scientific Reports
7 (2017).
date_created: 2018-12-11T11:50:12Z
date_published: 2017-02-06T00:00:00Z
date_updated: 2023-09-20T11:35:35Z
day: '06'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.1038/srep41906
ec_funded: 1
external_id:
isi:
- '000393367600001'
file:
- access_level: open_access
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:18:09Z
date_updated: 2018-12-12T10:18:09Z
file_id: '5328'
file_name: IST-2017-803-v1+1_srep41906.pdf
file_size: 1654496
relation: main_file
file_date_updated: 2018-12-12T10:18:09Z
has_accepted_license: '1'
intvolume: ' 7'
isi: 1
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Scientific Reports
publication_identifier:
issn:
- '20452322'
publication_status: published
publisher: Nature Publishing Group
publist_id: '6258'
pubrep_id: '803'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Flavonol-induced changes in PIN2 polarity and auxin transport in the Arabidopsis
thaliana rol1-2 mutant require phosphatase activity
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 7
year: '2017'
...
---
_id: '799'
abstract:
- lang: eng
text: Membrane traffic at the trans-Golgi network (TGN) is crucial for correctly
distributing various membrane proteins to their destination. Polarly localized
auxin efflux proteins, including PIN-FORMED1 (PIN1), are dynamically transported
between the endosomes and the plasma membrane (PM) in the plant cells. The intracellular
trafficking of PIN1 protein is sensitive to a fungal toxin brefeldin A (BFA),
which is known to inhibit guanine-nucleotide exchange factors for ADP ribosylation
factors (ARF GEFs) such as GNOM. However, the molecular details of the BFA-sensitive
trafficking pathway have not been revealed fully. In a previous study, we have
identified an Arabidopsis mutant BFA-visualized endocytic trafficking defective
3 (ben3) which exhibited reduced sensitivity to BFA in terms of BFA-induced intracellular
PIN1 agglomeration. Here, we show that BEN3 encodes a member of BIG family ARF
GEFs, BIG2. Fluorescent proteins tagged BEN3/BIG2 co-localized with markers for
TGN / early endosome (EE). Inspection of conditionally induced de novo synthesized
PIN1 confirmed that its secretion to the PM is BFA-sensitive and established BEN3/BIG2
as a crucial component of this BFA action at the level of TGN/EE. Furthermore,
ben3 mutation alleviated BFA-induced agglomeration of another TGN-localized ARF
GEF BEN1/MIN7. Taken together our results suggest that BEN3/BIG2 is an ARF GEF
component, which confers BFA sensitivity to the TGN/EE in Arabidopsis.
article_number: 1801-1811
article_processing_charge: No
author:
- first_name: Saeko
full_name: Kitakura, Saeko
last_name: Kitakura
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Yuki
full_name: Matsuura, Yuki
last_name: Matsuura
- first_name: Luca
full_name: Santuari, Luca
last_name: Santuari
- first_name: Hirotaka
full_name: Kouno, Hirotaka
last_name: Kouno
- first_name: Kohei
full_name: Arima, Kohei
last_name: Arima
- first_name: Christian
full_name: Hardtke, Christian
last_name: Hardtke
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Tatsuo
full_name: Kakimoto, Tatsuo
last_name: Kakimoto
- first_name: Hirokazu
full_name: Tanaka, Hirokazu
last_name: Tanaka
citation:
ama: Kitakura S, Adamowski M, Matsuura Y, et al. BEN3/BIG2 ARF GEF is involved in
brefeldin a-sensitive trafficking at the trans-Golgi network/early endosome in
Arabidopsis thaliana. Plant and Cell Physiology. 2017;58(10). doi:10.1093/pcp/pcx118
apa: Kitakura, S., Adamowski, M., Matsuura, Y., Santuari, L., Kouno, H., Arima,
K., … Tanaka, H. (2017). BEN3/BIG2 ARF GEF is involved in brefeldin a-sensitive
trafficking at the trans-Golgi network/early endosome in Arabidopsis thaliana.
Plant and Cell Physiology. Oxford University Press. https://doi.org/10.1093/pcp/pcx118
chicago: Kitakura, Saeko, Maciek Adamowski, Yuki Matsuura, Luca Santuari, Hirotaka
Kouno, Kohei Arima, Christian Hardtke, Jiří Friml, Tatsuo Kakimoto, and Hirokazu
Tanaka. “BEN3/BIG2 ARF GEF Is Involved in Brefeldin a-Sensitive Trafficking at
the Trans-Golgi Network/Early Endosome in Arabidopsis Thaliana.” Plant and
Cell Physiology. Oxford University Press, 2017. https://doi.org/10.1093/pcp/pcx118.
ieee: S. Kitakura et al., “BEN3/BIG2 ARF GEF is involved in brefeldin a-sensitive
trafficking at the trans-Golgi network/early endosome in Arabidopsis thaliana,”
Plant and Cell Physiology, vol. 58, no. 10. Oxford University Press, 2017.
ista: Kitakura S, Adamowski M, Matsuura Y, Santuari L, Kouno H, Arima K, Hardtke
C, Friml J, Kakimoto T, Tanaka H. 2017. BEN3/BIG2 ARF GEF is involved in brefeldin
a-sensitive trafficking at the trans-Golgi network/early endosome in Arabidopsis
thaliana. Plant and Cell Physiology. 58(10), 1801–1811.
mla: Kitakura, Saeko, et al. “BEN3/BIG2 ARF GEF Is Involved in Brefeldin a-Sensitive
Trafficking at the Trans-Golgi Network/Early Endosome in Arabidopsis Thaliana.”
Plant and Cell Physiology, vol. 58, no. 10, 1801–1811, Oxford University
Press, 2017, doi:10.1093/pcp/pcx118.
short: S. Kitakura, M. Adamowski, Y. Matsuura, L. Santuari, H. Kouno, K. Arima,
C. Hardtke, J. Friml, T. Kakimoto, H. Tanaka, Plant and Cell Physiology 58 (2017).
date_created: 2018-12-11T11:48:34Z
date_published: 2017-08-21T00:00:00Z
date_updated: 2023-09-27T11:00:19Z
day: '21'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.1093/pcp/pcx118
external_id:
isi:
- '000413220400019'
pmid:
- '29016942'
file:
- access_level: open_access
checksum: bd3e3a94d55416739cbb19624bb977f8
content_type: application/pdf
creator: dernst
date_created: 2019-04-17T07:52:34Z
date_updated: 2020-07-14T12:48:06Z
file_id: '6333'
file_name: 2017_PlantCellPhysio_Kitakura.pdf
file_size: 1352913
relation: main_file
file_date_updated: 2020-07-14T12:48:06Z
has_accepted_license: '1'
intvolume: ' 58'
isi: 1
issue: '10'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Submitted Version
pmid: 1
publication: Plant and Cell Physiology
publication_identifier:
issn:
- '00320781'
publication_status: published
publisher: Oxford University Press
publist_id: '6854'
pubrep_id: '1009'
quality_controlled: '1'
scopus_import: '1'
status: public
title: BEN3/BIG2 ARF GEF is involved in brefeldin a-sensitive trafficking at the trans-Golgi
network/early endosome in Arabidopsis thaliana
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 58
year: '2017'
...
---
_id: '545'
abstract:
- lang: eng
text: Development of vascular tissue is a remarkable example of intercellular communication
and coordinated development involving hormonal signaling and tissue polarity.
Thus far, studies on vascular patterning and regeneration have been conducted
mainly in trees—woody plants—with a well-developed layer of vascular cambium and
secondary tissues. Trees are difficult to use as genetic models, i.e., due to
long generation time, unstable environmental conditions, and lack of available
mutants and transgenic lines. Therefore, the use of the main genetic model plant
Arabidopsis thaliana (L.) Heynh., with a wealth of available marker and transgenic
lines, provides a unique opportunity to address molecular mechanism of vascular
tissue formation and regeneration. With specific treatments, the tiny weed Arabidopsis
can serve as a model to understand the growth of mighty trees and interconnect
a tree physiology with molecular genetics and cell biology of Arabidopsis.
alternative_title:
- Agricultural and Biological Sciences
author:
- first_name: Ewa
full_name: Mazur, Ewa
last_name: Mazur
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Mazur E, Friml J. Vascular tissue development and regeneration in the model
plant arabidopsis. In: Jurić S, ed. Plant Engineering. Plant Engineering.
InTech; 2017:113-140. doi:10.5772/intechopen.69712'
apa: Mazur, E., & Friml, J. (2017). Vascular tissue development and regeneration
in the model plant arabidopsis. In S. Jurić (Ed.), Plant Engineering (pp.
113–140). InTech. https://doi.org/10.5772/intechopen.69712
chicago: Mazur, Ewa, and Jiří Friml. “Vascular Tissue Development and Regeneration
in the Model Plant Arabidopsis.” In Plant Engineering, edited by Snježana
Jurić, 113–40. Plant Engineering. InTech, 2017. https://doi.org/10.5772/intechopen.69712.
ieee: E. Mazur and J. Friml, “Vascular tissue development and regeneration in the
model plant arabidopsis,” in Plant Engineering, S. Jurić, Ed. InTech, 2017,
pp. 113–140.
ista: 'Mazur E, Friml J. 2017.Vascular tissue development and regeneration in the
model plant arabidopsis. In: Plant Engineering. Agricultural and Biological Sciences,
, 113–140.'
mla: Mazur, Ewa, and Jiří Friml. “Vascular Tissue Development and Regeneration in
the Model Plant Arabidopsis.” Plant Engineering, edited by Snježana Jurić,
InTech, 2017, pp. 113–40, doi:10.5772/intechopen.69712.
short: E. Mazur, J. Friml, in:, S. Jurić (Ed.), Plant Engineering, InTech, 2017,
pp. 113–140.
date_created: 2018-12-11T11:47:05Z
date_published: 2017-11-17T00:00:00Z
date_updated: 2024-02-12T12:03:42Z
day: '17'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.5772/intechopen.69712
ec_funded: 1
editor:
- first_name: Snježana
full_name: Jurić, Snježana
last_name: Jurić
file:
- access_level: open_access
checksum: e1f05e5850dfd9f9434d2d373ca61941
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:12:49Z
date_updated: 2020-07-14T12:46:58Z
file_id: '4969'
file_name: IST-2018-929-v1+1_56106.pdf
file_size: 7443683
relation: main_file
file_date_updated: 2020-07-14T12:46:58Z
has_accepted_license: '1'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
page: 113 - 140
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Plant Engineering
publication_status: published
publisher: InTech
publist_id: '7269'
pubrep_id: '929'
quality_controlled: '1'
related_material:
record:
- id: '1274'
relation: earlier_version
status: public
series_title: Plant Engineering
status: public
title: Vascular tissue development and regeneration in the model plant arabidopsis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: book_chapter
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
year: '2017'
...
---
_id: '946'
abstract:
- lang: eng
text: Roots navigate through soil integrating environmental signals to orient their
growth. The Arabidopsis root is a widely used model for developmental, physiological
and cell biological studies. Live imaging greatly aids these efforts, but the
horizontal sample position and continuous root tip displacement present significant
difficulties. Here, we develop a confocal microscope setup for vertical sample
mounting and integrated directional illumination. We present TipTracker – a custom
software for automatic tracking of diverse moving objects usable on various microscope
setups. Combined, this enables observation of root tips growing along the natural
gravity vector over prolonged periods of time, as well as the ability to induce
rapid gravity or light stimulation. We also track migrating cells in the developing
zebrafish embryo, demonstrating the utility of this system in the acquisition
of high-resolution data sets of dynamic samples. We provide detailed descriptions
of the tools enabling the easy implementation on other microscopes.
acknowledged_ssus:
- _id: M-Shop
- _id: Bio
acknowledgement: "Funding: Marie Curie Actions (FP7/2007-2013 no 291734) to Daniel
von Wangenheim; Austrian Science Fund (M 2128-B21) to Matyáš Fendrych; Austrian
Science Fund (FWF01_I1774S) to Eva Benková; European Research Council (FP7/2007-2013
no 282300) to Jiří Friml. \r\nThe authors are grateful to the Miba Machine Shop
at IST Austria for their contribution to the microscope setup and to Yvonne Kemper
for reading, understanding and correcting the manuscript.\r\n#BioimagingFacility"
article_number: e26792
article_processing_charge: Yes
author:
- first_name: Daniel
full_name: Von Wangenheim, Daniel
id: 49E91952-F248-11E8-B48F-1D18A9856A87
last_name: Von Wangenheim
orcid: 0000-0002-6862-1247
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: Vanessa
full_name: Barone, Vanessa
id: 419EECCC-F248-11E8-B48F-1D18A9856A87
last_name: Barone
orcid: 0000-0003-2676-3367
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: von Wangenheim D, Hauschild R, Fendrych M, Barone V, Benková E, Friml J. Live
tracking of moving samples in confocal microscopy for vertically grown roots.
eLife. 2017;6. doi:10.7554/eLife.26792
apa: von Wangenheim, D., Hauschild, R., Fendrych, M., Barone, V., Benková, E., &
Friml, J. (2017). Live tracking of moving samples in confocal microscopy for vertically
grown roots. ELife. eLife Sciences Publications. https://doi.org/10.7554/eLife.26792
chicago: Wangenheim, Daniel von, Robert Hauschild, Matyas Fendrych, Vanessa Barone,
Eva Benková, and Jiří Friml. “Live Tracking of Moving Samples in Confocal Microscopy
for Vertically Grown Roots.” ELife. eLife Sciences Publications, 2017.
https://doi.org/10.7554/eLife.26792.
ieee: D. von Wangenheim, R. Hauschild, M. Fendrych, V. Barone, E. Benková, and J.
Friml, “Live tracking of moving samples in confocal microscopy for vertically
grown roots,” eLife, vol. 6. eLife Sciences Publications, 2017.
ista: von Wangenheim D, Hauschild R, Fendrych M, Barone V, Benková E, Friml J. 2017.
Live tracking of moving samples in confocal microscopy for vertically grown roots.
eLife. 6, e26792.
mla: von Wangenheim, Daniel, et al. “Live Tracking of Moving Samples in Confocal
Microscopy for Vertically Grown Roots.” ELife, vol. 6, e26792, eLife Sciences
Publications, 2017, doi:10.7554/eLife.26792.
short: D. von Wangenheim, R. Hauschild, M. Fendrych, V. Barone, E. Benková, J. Friml,
ELife 6 (2017).
date_created: 2018-12-11T11:49:21Z
date_published: 2017-06-19T00:00:00Z
date_updated: 2024-02-21T13:49:34Z
day: '19'
ddc:
- '570'
department:
- _id: JiFr
- _id: Bio
- _id: CaHe
- _id: EvBe
doi: 10.7554/eLife.26792
ec_funded: 1
external_id:
isi:
- '000404728300001'
file:
- access_level: open_access
checksum: 9af3398cb0d81f99d79016a616df22e9
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:17:57Z
date_updated: 2020-07-14T12:48:15Z
file_id: '5315'
file_name: IST-2017-847-v1+1_elife-26792-v2.pdf
file_size: 19581847
relation: main_file
file_date_updated: 2020-07-14T12:48:15Z
has_accepted_license: '1'
intvolume: ' 6'
isi: 1
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
project:
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
- _id: 2572ED28-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: M02128
name: Molecular basis of root growth inhibition by auxin
- _id: 2542D156-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I 1774-B16
name: Hormone cross-talk drives nutrient dependent plant development
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: eLife
publication_status: published
publisher: eLife Sciences Publications
publist_id: '6471'
pubrep_id: '847'
quality_controlled: '1'
related_material:
record:
- id: '5566'
relation: popular_science
status: public
scopus_import: '1'
status: public
title: Live tracking of moving samples in confocal microscopy for vertically grown
roots
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 6
year: '2017'
...
---
_id: '1078'
abstract:
- lang: eng
text: 'One of the key questions in understanding plant development is how single
cells behave in a larger context of the tissue. Therefore, it requires the observation
of the whole organ with a high spatial- as well as temporal resolution over prolonged
periods of time, which may cause photo-toxic effects. This protocol shows a plant
sample preparation method for light-sheet microscopy, which is characterized by
mounting the plant vertically on the surface of a gel. The plant is mounted in
such a way that the roots are submerged in a liquid medium while the leaves remain
in the air. In order to ensure photosynthetic activity of the plant, a custom-made
lighting system illuminates the leaves. To keep the roots in darkness the water
surface is covered with sheets of black plastic foil. This method allows long-term
imaging of plant organ development in standardized conditions. '
acknowledged_ssus:
- _id: M-Shop
- _id: Bio
article_number: e55044
article_processing_charge: No
author:
- first_name: Daniel
full_name: Von Wangenheim, Daniel
id: 49E91952-F248-11E8-B48F-1D18A9856A87
last_name: Von Wangenheim
orcid: 0000-0002-6862-1247
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: von Wangenheim D, Hauschild R, Friml J. Light sheet fluorescence microscopy
of plant roots growing on the surface of a gel. Journal of visualized experiments
JoVE. 2017;2017(119). doi:10.3791/55044
apa: von Wangenheim, D., Hauschild, R., & Friml, J. (2017). Light sheet fluorescence
microscopy of plant roots growing on the surface of a gel. Journal of Visualized
Experiments JoVE. Journal of Visualized Experiments. https://doi.org/10.3791/55044
chicago: Wangenheim, Daniel von, Robert Hauschild, and Jiří Friml. “Light Sheet
Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel.” Journal
of Visualized Experiments JoVE. Journal of Visualized Experiments, 2017. https://doi.org/10.3791/55044.
ieee: D. von Wangenheim, R. Hauschild, and J. Friml, “Light sheet fluorescence microscopy
of plant roots growing on the surface of a gel,” Journal of visualized experiments
JoVE, vol. 2017, no. 119. Journal of Visualized Experiments, 2017.
ista: von Wangenheim D, Hauschild R, Friml J. 2017. Light sheet fluorescence microscopy
of plant roots growing on the surface of a gel. Journal of visualized experiments
JoVE. 2017(119), e55044.
mla: von Wangenheim, Daniel, et al. “Light Sheet Fluorescence Microscopy of Plant
Roots Growing on the Surface of a Gel.” Journal of Visualized Experiments JoVE,
vol. 2017, no. 119, e55044, Journal of Visualized Experiments, 2017, doi:10.3791/55044.
short: D. von Wangenheim, R. Hauschild, J. Friml, Journal of Visualized Experiments
JoVE 2017 (2017).
date_created: 2018-12-11T11:50:01Z
date_published: 2017-01-18T00:00:00Z
date_updated: 2024-02-21T13:49:12Z
day: '18'
ddc:
- '580'
department:
- _id: JiFr
- _id: Bio
doi: 10.3791/55044
ec_funded: 1
external_id:
isi:
- '000397847200041'
file:
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content_type: application/pdf
creator: system
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date_updated: 2018-12-12T10:16:31Z
file_id: '5219'
file_name: IST-2017-808-v1+1_2017_VWangenheim_list.pdf
file_size: 57678
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content_type: application/pdf
creator: system
date_created: 2018-12-12T10:16:32Z
date_updated: 2018-12-12T10:16:32Z
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intvolume: ' 2017'
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issue: '119'
language:
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month: '01'
oa: 1
oa_version: Published Version
project:
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- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Journal of visualized experiments JoVE
publication_status: published
publisher: Journal of Visualized Experiments
publist_id: '6302'
pubrep_id: '808'
related_material:
record:
- id: '5565'
relation: popular_science
status: public
scopus_import: '1'
status: public
title: Light sheet fluorescence microscopy of plant roots growing on the surface of
a gel
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 2017
year: '2017'
...
---
_id: '5565'
abstract:
- lang: eng
text: "One of the key questions in understanding plant development is how single
cells behave in a larger context of the tissue. Therefore, it requires the observation
of the whole organ with a high spatial- as well as temporal resolution over prolonged
periods of time, which may cause photo-toxic effects. This protocol shows a plant
sample preparation method for light-sheet microscopy, which is characterized by
mounting the plant vertically on the surface of a gel. The plant is mounted in
such a way that the roots are submerged in a liquid medium while the leaves remain
in the air. In order to ensure photosynthetic activity of the plant, a custom-made
lighting system illuminates the leaves. To keep the roots in darkness the water
surface is covered with sheets of black plastic foil. This method allows long-term
imaging of plant organ development in standardized conditions. \r\nThe Video is
licensed under a CC BY NC ND license. "
acknowledgement: 'fund: FP7-ERC 0101109'
article_processing_charge: No
author:
- first_name: Daniel
full_name: Von Wangenheim, Daniel
id: 49E91952-F248-11E8-B48F-1D18A9856A87
last_name: Von Wangenheim
orcid: 0000-0002-6862-1247
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: von Wangenheim D, Hauschild R, Friml J. Light Sheet Fluorescence microscopy
of plant roots growing on the surface of a gel. 2017. doi:10.15479/AT:ISTA:66
apa: von Wangenheim, D., Hauschild, R., & Friml, J. (2017). Light Sheet Fluorescence
microscopy of plant roots growing on the surface of a gel. Institute of Science
and Technology Austria. https://doi.org/10.15479/AT:ISTA:66
chicago: Wangenheim, Daniel von, Robert Hauschild, and Jiří Friml. “Light Sheet
Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel.” Institute
of Science and Technology Austria, 2017. https://doi.org/10.15479/AT:ISTA:66.
ieee: D. von Wangenheim, R. Hauschild, and J. Friml, “Light Sheet Fluorescence microscopy
of plant roots growing on the surface of a gel.” Institute of Science and Technology
Austria, 2017.
ista: von Wangenheim D, Hauschild R, Friml J. 2017. Light Sheet Fluorescence microscopy
of plant roots growing on the surface of a gel, Institute of Science and Technology
Austria, 10.15479/AT:ISTA:66.
mla: von Wangenheim, Daniel, et al. Light Sheet Fluorescence Microscopy of Plant
Roots Growing on the Surface of a Gel. Institute of Science and Technology
Austria, 2017, doi:10.15479/AT:ISTA:66.
short: D. von Wangenheim, R. Hauschild, J. Friml, (2017).
datarep_id: '66'
date_created: 2018-12-12T12:31:34Z
date_published: 2017-04-10T00:00:00Z
date_updated: 2024-02-21T13:49:13Z
day: '10'
ddc:
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department:
- _id: JiFr
- _id: Bio
doi: 10.15479/AT:ISTA:66
ec_funded: 1
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content_type: video/mp4
creator: system
date_created: 2018-12-12T13:02:33Z
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month: '04'
oa: 1
oa_version: Published Version
project:
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
publisher: Institute of Science and Technology Austria
publist_id: '6302'
related_material:
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relation: research_paper
status: public
status: public
title: Light Sheet Fluorescence microscopy of plant roots growing on the surface of
a gel
type: research_data
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2017'
...
---
_id: '1081'
abstract:
- lang: eng
text: The asymmetric localization of proteins in the plasma membrane domains of
eukaryotic cells is a fundamental manifestation of cell polarity that is central
to multicellular organization and developmental patterning. In plants, the mechanisms
underlying the polar localization of cargo proteins are still largely unknown
and appear to be fundamentally distinct from those operating in mammals. Here,
we present a systematic, quantitative comparative analysis of the polar delivery
and subcellular localization of proteins that characterize distinct polar plasma
membrane domains in plant cells. The combination of microscopic analyses and computational
modeling revealed a mechanistic framework common to diverse polar cargos and underlying
the establishment and maintenance of apical, basal, and lateral polar domains
in plant cells. This mechanism depends on the polar secretion, constitutive endocytic
recycling, and restricted lateral diffusion of cargos within the plasma membrane.
Moreover, our observations suggest that polar cargo distribution involves the
individual protein potential to form clusters within the plasma membrane and interact
with the extracellular matrix. Our observations provide insights into the shared
cellular mechanisms of polar cargo delivery and polarity maintenance in plant
cells.
acknowledgement: "We thank Bonnie Bartel, Jenny Russinova and Niko Geldner\r\nfor
sharing published material, Martine de Cock and Annick\r\nBleys for help in preparing
the manuscript. This work was\r\nsupported by the European Research Council (project\r\nERC-2011-StG-20101109-PSDP);
Czech Science Foundation\r\nGAČR (GA13-40637S); project CEITEC—Central European\r\nInstitute
of Technology (CZ.1.05/1.1.00/02.0068). SV is a\r\npostdoctoral fellow of the Research
Foundation-Flanders.\r\nSN is a Project Assistant Professor supported by the Japanese\r\nSociety
for the Promotion of Science (JSPS; 30612022 to SN),\r\nthe NC-CARP project of the
Ministry of Education, Culture,\r\nSports, Science and Technology in Japan to SN."
article_number: '16018'
author:
- first_name: Łukasz
full_name: Łangowski, Łukasz
last_name: Łangowski
- first_name: Krzysztof T
full_name: Wabnik, Krzysztof T
id: 4DE369A4-F248-11E8-B48F-1D18A9856A87
last_name: Wabnik
orcid: 0000-0001-7263-0560
- first_name: Hongjiang
full_name: Li, Hongjiang
id: 33CA54A6-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0001-5039-9660
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Satoshi
full_name: Naramoto, Satoshi
last_name: Naramoto
- first_name: Hirokazu
full_name: Tanaka, Hirokazu
last_name: Tanaka
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Łangowski Ł, Wabnik KT, Li H, et al. Cellular mechanisms for cargo delivery
and polarity maintenance at different polar domains in plant cells. Cell Discovery.
2016;2. doi:10.1038/celldisc.2016.18
apa: Łangowski, Ł., Wabnik, K. T., Li, H., Vanneste, S., Naramoto, S., Tanaka, H.,
& Friml, J. (2016). Cellular mechanisms for cargo delivery and polarity maintenance
at different polar domains in plant cells. Cell Discovery. Nature Publishing
Group. https://doi.org/10.1038/celldisc.2016.18
chicago: Łangowski, Łukasz, Krzysztof T Wabnik, Hongjiang Li, Steffen Vanneste,
Satoshi Naramoto, Hirokazu Tanaka, and Jiří Friml. “Cellular Mechanisms for Cargo
Delivery and Polarity Maintenance at Different Polar Domains in Plant Cells.”
Cell Discovery. Nature Publishing Group, 2016. https://doi.org/10.1038/celldisc.2016.18.
ieee: Ł. Łangowski et al., “Cellular mechanisms for cargo delivery and polarity
maintenance at different polar domains in plant cells,” Cell Discovery,
vol. 2. Nature Publishing Group, 2016.
ista: Łangowski Ł, Wabnik KT, Li H, Vanneste S, Naramoto S, Tanaka H, Friml J. 2016.
Cellular mechanisms for cargo delivery and polarity maintenance at different polar
domains in plant cells. Cell Discovery. 2, 16018.
mla: Łangowski, Łukasz, et al. “Cellular Mechanisms for Cargo Delivery and Polarity
Maintenance at Different Polar Domains in Plant Cells.” Cell Discovery,
vol. 2, 16018, Nature Publishing Group, 2016, doi:10.1038/celldisc.2016.18.
short: Ł. Łangowski, K.T. Wabnik, H. Li, S. Vanneste, S. Naramoto, H. Tanaka, J.
Friml, Cell Discovery 2 (2016).
date_created: 2018-12-11T11:50:02Z
date_published: 2016-07-19T00:00:00Z
date_updated: 2021-01-12T06:48:08Z
day: '19'
ddc:
- '580'
department:
- _id: EvBe
- _id: JiFr
doi: 10.1038/celldisc.2016.18
ec_funded: 1
file:
- access_level: open_access
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:13:33Z
date_updated: 2018-12-12T10:13:33Z
file_id: '5017'
file_name: IST-2017-757-v1+1_celldisc201618.pdf
file_size: 5261671
relation: main_file
file_date_updated: 2018-12-12T10:13:33Z
has_accepted_license: '1'
intvolume: ' 2'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Cell Discovery
publication_status: published
publisher: Nature Publishing Group
publist_id: '6299'
pubrep_id: '757'
quality_controlled: '1'
scopus_import: 1
status: public
title: Cellular mechanisms for cargo delivery and polarity maintenance at different
polar domains in plant cells
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 2
year: '2016'
...
---
_id: '1145'
abstract:
- lang: eng
text: Auxin directs plant ontogenesis via differential accumulation within tissues
depending largely on the activity of PIN proteins that mediate auxin efflux from
cells and its directional cell-to-cell transport. Regardless of the developmental
importance of PINs, the structure of these transporters is poorly characterized.
Here, we present experimental data concerning protein topology of plasma membrane-localized
PINs. Utilizing approaches based on pH-dependent quenching of fluorescent reporters
combined with immunolocalization techniques, we mapped the membrane topology of
PINs and further cross-validated our results using available topology modeling
software. We delineated the topology of PIN1 with two transmembrane (TM) bundles
of five α-helices linked by a large intracellular loop and a C-terminus positioned
outside the cytoplasm. Using constraints derived from our experimental data, we
also provide an updated position of helical regions generating a verisimilitude
model of PIN1. Since the canonical long PINs show a high degree of conservation
in TM domains and auxin transport capacity has been demonstrated for Arabidopsis
representatives of this group, this empirically enhanced topological model of
PIN1 will be an important starting point for further studies on PIN structure–function
relationships. In addition, we have established protocols that can be used to
probe the topology of other plasma membrane proteins in plants. © 2016 The Authors
acknowledgement: This research has been financially supported by the Ministry of Education,
Youth and Sports of the Czech Republic under the project CEITEC 2020 (LQ1601) (T.N.,
M.Z., M.P., J.H.), Czech Science Foundation (13-40637S [J.F., M.Z.], 13-39982S [J.H.]);
Research Foundation Flanders (Grant number FWO09/PDO/196) (S.V.) and the European
Research Council (project ERC-2011-StG-20101109-PSDP) (J.F.). We thank David G.
Robinson and Ranjan Swarup for sharing published material; Maria Šimášková, Mamoona
Khan, Eva Benková for technical assistance; and R. Tejos, J. Kleine-Vehn, and E.
Feraru for helpful discussions.
author:
- first_name: Tomasz
full_name: Nodzyński, Tomasz
last_name: Nodzyński
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Marta
full_name: Zwiewka, Marta
last_name: Zwiewka
- first_name: Markéta
full_name: Pernisová, Markéta
last_name: Pernisová
- first_name: Jan
full_name: Hejátko, Jan
last_name: Hejátko
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Nodzyński T, Vanneste S, Zwiewka M, Pernisová M, Hejátko J, Friml J. Enquiry
into the topology of plasma membrane localized PIN auxin transport components.
Molecular Plant. 2016;9(11):1504-1519. doi:10.1016/j.molp.2016.08.010
apa: Nodzyński, T., Vanneste, S., Zwiewka, M., Pernisová, M., Hejátko, J., &
Friml, J. (2016). Enquiry into the topology of plasma membrane localized PIN auxin
transport components. Molecular Plant. Cell Press. https://doi.org/10.1016/j.molp.2016.08.010
chicago: Nodzyński, Tomasz, Steffen Vanneste, Marta Zwiewka, Markéta Pernisová,
Jan Hejátko, and Jiří Friml. “Enquiry into the Topology of Plasma Membrane Localized
PIN Auxin Transport Components.” Molecular Plant. Cell Press, 2016. https://doi.org/10.1016/j.molp.2016.08.010.
ieee: T. Nodzyński, S. Vanneste, M. Zwiewka, M. Pernisová, J. Hejátko, and J. Friml,
“Enquiry into the topology of plasma membrane localized PIN auxin transport components,”
Molecular Plant, vol. 9, no. 11. Cell Press, pp. 1504–1519, 2016.
ista: Nodzyński T, Vanneste S, Zwiewka M, Pernisová M, Hejátko J, Friml J. 2016.
Enquiry into the topology of plasma membrane localized PIN auxin transport components.
Molecular Plant. 9(11), 1504–1519.
mla: Nodzyński, Tomasz, et al. “Enquiry into the Topology of Plasma Membrane Localized
PIN Auxin Transport Components.” Molecular Plant, vol. 9, no. 11, Cell
Press, 2016, pp. 1504–19, doi:10.1016/j.molp.2016.08.010.
short: T. Nodzyński, S. Vanneste, M. Zwiewka, M. Pernisová, J. Hejátko, J. Friml,
Molecular Plant 9 (2016) 1504–1519.
date_created: 2018-12-11T11:50:23Z
date_published: 2016-11-07T00:00:00Z
date_updated: 2021-01-12T06:48:37Z
day: '07'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.1016/j.molp.2016.08.010
ec_funded: 1
file:
- access_level: open_access
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:13:22Z
date_updated: 2018-12-12T10:13:22Z
file_id: '5004'
file_name: IST-2017-746-v1+1_1-s2.0-S1674205216301915-main.pdf
file_size: 5005876
relation: main_file
file_date_updated: 2018-12-12T10:13:22Z
has_accepted_license: '1'
intvolume: ' 9'
issue: '11'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
page: 1504 - 1519
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Molecular Plant
publication_status: published
publisher: Cell Press
publist_id: '6213'
pubrep_id: '746'
quality_controlled: '1'
scopus_import: 1
status: public
title: Enquiry into the topology of plasma membrane localized PIN auxin transport
components
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 9
year: '2016'
...
---
_id: '1147'
abstract:
- lang: eng
text: Apical dominance is one of the fundamental developmental phenomena in plant
biology, which determines the overall architecture of aerial plant parts. Here
we show apex decapitation activated competition for dominance in adjacent upper
and lower axillary buds. A two-nodal-bud pea (Pisum sativum L.) was used as a
model system to monitor and assess auxin flow, auxin transport channels, and dormancy
and initiation status of axillary buds. Auxin flow was manipulated by lateral
stem wounds or chemically by auxin efflux inhibitors 2,3,5-triiodobenzoic acid
(TIBA), 1-N-naphtylphtalamic acid (NPA), or protein synthesis inhibitor cycloheximide
(CHX) treatments, which served to interfere with axillary bud competition. Redirecting
auxin flow to different points influenced which bud formed the outgrowing and
dominant shoot. The obtained results proved that competition between upper and
lower axillary buds as secondary auxin sources is based on the same auxin canalization
principle that operates between the shoot apex and axillary bud. © The Author(s)
2016.
acknowledgement: This research was carried out under the project CEITEC 2020 (LQ1601)
with financial support from the Ministry of Education, Youth and Sports of the Czech
Republic under the National Sustainability Programme II., supported by the project
“CEITEC–Central European Institute of Technology” (CZ.1.05/1.1.00/02.0068) and the
Agronomy faculty grant from Mendel University “IGA AF MENDELU” (IP 14/2013).
article_number: '35955'
author:
- first_name: Jozef
full_name: Balla, Jozef
last_name: Balla
- first_name: Zuzana
full_name: Medved'Ová, Zuzana
last_name: Medved'Ová
- first_name: Petr
full_name: Kalousek, Petr
last_name: Kalousek
- first_name: Natálie
full_name: Matiješčuková, Natálie
last_name: Matiješčuková
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Vilém
full_name: Reinöhl, Vilém
last_name: Reinöhl
- first_name: Stanislav
full_name: Procházka, Stanislav
last_name: Procházka
citation:
ama: Balla J, Medved’Ová Z, Kalousek P, et al. Auxin flow mediated competition between
axillary buds to restore apical dominance. Scientific Reports. 2016;6.
doi:10.1038/srep35955
apa: Balla, J., Medved’Ová, Z., Kalousek, P., Matiješčuková, N., Friml, J., Reinöhl,
V., & Procházka, S. (2016). Auxin flow mediated competition between axillary
buds to restore apical dominance. Scientific Reports. Nature Publishing
Group. https://doi.org/10.1038/srep35955
chicago: Balla, Jozef, Zuzana Medved’Ová, Petr Kalousek, Natálie Matiješčuková,
Jiří Friml, Vilém Reinöhl, and Stanislav Procházka. “Auxin Flow Mediated Competition
between Axillary Buds to Restore Apical Dominance.” Scientific Reports.
Nature Publishing Group, 2016. https://doi.org/10.1038/srep35955.
ieee: J. Balla et al., “Auxin flow mediated competition between axillary
buds to restore apical dominance,” Scientific Reports, vol. 6. Nature Publishing
Group, 2016.
ista: Balla J, Medved’Ová Z, Kalousek P, Matiješčuková N, Friml J, Reinöhl V, Procházka
S. 2016. Auxin flow mediated competition between axillary buds to restore apical
dominance. Scientific Reports. 6, 35955.
mla: Balla, Jozef, et al. “Auxin Flow Mediated Competition between Axillary Buds
to Restore Apical Dominance.” Scientific Reports, vol. 6, 35955, Nature
Publishing Group, 2016, doi:10.1038/srep35955.
short: J. Balla, Z. Medved’Ová, P. Kalousek, N. Matiješčuková, J. Friml, V. Reinöhl,
S. Procházka, Scientific Reports 6 (2016).
date_created: 2018-12-11T11:50:24Z
date_published: 2016-11-08T00:00:00Z
date_updated: 2021-01-12T06:48:38Z
day: '08'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.1038/srep35955
file:
- access_level: open_access
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:09:28Z
date_updated: 2018-12-12T10:09:28Z
file_id: '4752'
file_name: IST-2017-745-v1+1_srep35955.pdf
file_size: 1587544
relation: main_file
file_date_updated: 2018-12-12T10:09:28Z
has_accepted_license: '1'
intvolume: ' 6'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
publication: Scientific Reports
publication_status: published
publisher: Nature Publishing Group
publist_id: '6211'
pubrep_id: '745'
quality_controlled: '1'
scopus_import: 1
status: public
title: Auxin flow mediated competition between axillary buds to restore apical dominance
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 6
year: '2016'
...
---
_id: '1151'
abstract:
- lang: eng
text: Tissue patterning in multicellular organisms is the output of precise spatio–temporal
regulation of gene expression coupled with changes in hormone dynamics. In plants,
the hormone auxin regulates growth and development at every stage of a plant’s
life cycle. Auxin signaling occurs through binding of the auxin molecule to a
TIR1/AFB F-box ubiquitin ligase, allowing interaction with Aux/IAA transcriptional
repressor proteins. These are subsequently ubiquitinated and degraded via the
26S proteasome, leading to derepression of auxin response factors (ARFs). How
auxin is able to elicit such a diverse range of developmental responses through
a single signaling module has not yet been resolved. Here we present an alternative
auxin-sensing mechanism in which the ARF ARF3/ETTIN controls gene expression through
interactions with process-specific transcription factors. This noncanonical hormonesensing
mechanism exhibits strong preference for the naturally occurring auxin indole
3-acetic acid (IAA) and is important for coordinating growth and patterning in
diverse developmental contexts such as gynoecium morphogenesis, lateral root emergence,
ovule development, and primary branch formation. Disrupting this IAA-sensing ability
induces morphological aberrations with consequences for plant fitness. Therefore,
our findings introduce a novel transcription factor-based mechanism of hormone
perception in plants. © 2016 Simonini et al.
acknowledgement: "We thank Norwich Research Park Bioimaging, Grant Calder, Roy\r\nDunford,
Caroline Smith, Paul Thomas, and Mark Youles for\r\ntechnical support; Charlie Scutt,
Alejandro Ferrando, and George\r\nLomonossoff for plasmids; Toshiro Ito for seeds;
Brendan Davies\r\nand Barry Causier for the REGIA library; and Mark Buttner,\r\nSimona
Masiero, Fabio Rossi, Doris Wagner, and Jun Xiao for\r\nhelp and material. We are
also grateful to Stefano Bencivenga,\r\nMarie Brüser, Friederike Jantzen, Lukasz
Langowski, Xinran Li,\r\nand Nicola Stacey for discussions and helpful comments
on the\r\nmanuscript. This work was supported by grants BB/M004112/1\r\nand BB/I017232/1
(Crop Improvement Research Club) to L.Ø.\r\nfrom the Biotechnological and Biological
Sciences Research\r\nCouncil, and Institute Strategic Programme grant (BB/J004553/\r\n1)
to the John Innes Centre. S.S., J.D., and L.Ø conceived the ex-\r\nperiments. "
author:
- first_name: Sara
full_name: Simonini, Sara
last_name: Simonini
- first_name: Joyita
full_name: Deb, Joyita
last_name: Deb
- first_name: Laila
full_name: Moubayidin, Laila
last_name: Moubayidin
- first_name: Pauline
full_name: Stephenson, Pauline
last_name: Stephenson
- first_name: Manoj
full_name: Valluru, Manoj
last_name: Valluru
- first_name: Alejandra
full_name: Freire Rios, Alejandra
last_name: Freire Rios
- first_name: Karim
full_name: Sorefan, Karim
last_name: Sorefan
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Lars
full_name: Östergaard, Lars
last_name: Östergaard
citation:
ama: Simonini S, Deb J, Moubayidin L, et al. A noncanonical auxin sensing mechanism
is required for organ morphogenesis in arabidopsis. Genes and Development.
2016;30(20):2286-2296. doi:10.1101/gad.285361.116
apa: Simonini, S., Deb, J., Moubayidin, L., Stephenson, P., Valluru, M., Freire
Rios, A., … Östergaard, L. (2016). A noncanonical auxin sensing mechanism is required
for organ morphogenesis in arabidopsis. Genes and Development. Cold Spring
Harbor Laboratory Press. https://doi.org/10.1101/gad.285361.116
chicago: Simonini, Sara, Joyita Deb, Laila Moubayidin, Pauline Stephenson, Manoj
Valluru, Alejandra Freire Rios, Karim Sorefan, Dolf Weijers, Jiří Friml, and Lars
Östergaard. “A Noncanonical Auxin Sensing Mechanism Is Required for Organ Morphogenesis
in Arabidopsis.” Genes and Development. Cold Spring Harbor Laboratory Press,
2016. https://doi.org/10.1101/gad.285361.116.
ieee: S. Simonini et al., “A noncanonical auxin sensing mechanism is required
for organ morphogenesis in arabidopsis,” Genes and Development, vol. 30,
no. 20. Cold Spring Harbor Laboratory Press, pp. 2286–2296, 2016.
ista: Simonini S, Deb J, Moubayidin L, Stephenson P, Valluru M, Freire Rios A, Sorefan
K, Weijers D, Friml J, Östergaard L. 2016. A noncanonical auxin sensing mechanism
is required for organ morphogenesis in arabidopsis. Genes and Development. 30(20),
2286–2296.
mla: Simonini, Sara, et al. “A Noncanonical Auxin Sensing Mechanism Is Required
for Organ Morphogenesis in Arabidopsis.” Genes and Development, vol. 30,
no. 20, Cold Spring Harbor Laboratory Press, 2016, pp. 2286–96, doi:10.1101/gad.285361.116.
short: S. Simonini, J. Deb, L. Moubayidin, P. Stephenson, M. Valluru, A. Freire
Rios, K. Sorefan, D. Weijers, J. Friml, L. Östergaard, Genes and Development 30
(2016) 2286–2296.
date_created: 2018-12-11T11:50:25Z
date_published: 2016-10-15T00:00:00Z
date_updated: 2021-01-12T06:48:39Z
day: '15'
ddc:
- '570'
department:
- _id: JiFr
doi: 10.1101/gad.285361.116
external_id:
pmid:
- '27898393'
file:
- access_level: open_access
content_type: application/pdf
creator: dernst
date_created: 2019-01-25T09:32:55Z
date_updated: 2019-01-25T09:32:55Z
file_id: '5882'
file_name: 2016_GeneDev_Simonini.pdf
file_size: 1419263
relation: main_file
success: 1
file_date_updated: 2019-01-25T09:32:55Z
has_accepted_license: '1'
intvolume: ' 30'
issue: '20'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
page: 2286 - 2296
pmid: 1
publication: Genes and Development
publication_status: published
publisher: Cold Spring Harbor Laboratory Press
publist_id: '6207'
quality_controlled: '1'
scopus_import: 1
status: public
title: A noncanonical auxin sensing mechanism is required for organ morphogenesis
in arabidopsis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 30
year: '2016'
...
---
_id: '1153'
abstract:
- lang: eng
text: Differential cell growth enables flexible organ bending in the presence of
environmental signals such as light or gravity. A prominent example of the developmental
processes based on differential cell growth is the formation of the apical hook
that protects the fragile shoot apical meristem when it breaks through the soil
during germination. Here, we combined in silico and in vivo approaches to identify
a minimal mechanism producing auxin gradient-guided differential growth during
the establishment of the apical hook in the model plant Arabidopsis thaliana.
Computer simulation models based on experimental data demonstrate that asymmetric
expression of the PIN-FORMED auxin efflux carrier at the concave (inner) versus
convex (outer) side of the hook suffices to establish an auxin maximum in the
epidermis at the concave side of the apical hook. Furthermore, we propose a mechanism
that translates this maximum into differential growth, and thus curvature, of
the apical hook. Through a combination of experimental and in silico computational
approaches, we have identified the individual contributions of differential cell
elongation and proliferation to defining the apical hook and reveal the role of
auxin-ethylene crosstalk in balancing these two processes. © 2016 American Society
of Plant Biologists. All rights reserved.
acknowledgement: "We thank Martine De Cock and Annick Bleys for help in preparing
the manuscript, Daniel Van Damme for sharing material and stimulating discussion,
and Rudiger Simon for support during revision of the manuscript.\r\nThis work was
supported by grants from the European Research Council (StartingIndependentResearchGrantERC-2007-Stg-207362-HCPO)and
the Czech Science Foundation (GACR CZ.1.07/2.3.00/20.0043) to E.B.\r\nand Natural
Sciences and Engineering Research Council of Canada Discovery Grant 2014-05325 to
P.P. K.W. acknowledges funding from a Human Frontier Science Program Long-Term Fellowship
(LT-000209-2014)."
author:
- first_name: Petra
full_name: Žádníková, Petra
last_name: Žádníková
- first_name: Krzysztof T
full_name: Wabnik, Krzysztof T
id: 4DE369A4-F248-11E8-B48F-1D18A9856A87
last_name: Wabnik
orcid: 0000-0001-7263-0560
- first_name: Anas
full_name: Abuzeineh, Anas
last_name: Abuzeineh
- first_name: Marçal
full_name: Gallemí, Marçal
last_name: Gallemí
- first_name: Dominique
full_name: Van Der Straeten, Dominique
last_name: Van Der Straeten
- first_name: Richard
full_name: Smith, Richard
last_name: Smith
- first_name: Dirk
full_name: Inze, Dirk
last_name: Inze
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Przemysław
full_name: Prusinkiewicz, Przemysław
last_name: Prusinkiewicz
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Žádníková P, Wabnik KT, Abuzeineh A, et al. A model of differential growth
guided apical hook formation in plants. Plant Cell. 2016;28(10):2464-2477.
doi:10.1105/tpc.15.00569
apa: Žádníková, P., Wabnik, K. T., Abuzeineh, A., Gallemí, M., Van Der Straeten,
D., Smith, R., … Benková, E. (2016). A model of differential growth guided apical
hook formation in plants. Plant Cell. American Society of Plant Biologists.
https://doi.org/10.1105/tpc.15.00569
chicago: Žádníková, Petra, Krzysztof T Wabnik, Anas Abuzeineh, Marçal Gallemí, Dominique
Van Der Straeten, Richard Smith, Dirk Inze, Jiří Friml, Przemysław Prusinkiewicz,
and Eva Benková. “A Model of Differential Growth Guided Apical Hook Formation
in Plants.” Plant Cell. American Society of Plant Biologists, 2016. https://doi.org/10.1105/tpc.15.00569.
ieee: P. Žádníková et al., “A model of differential growth guided apical
hook formation in plants,” Plant Cell, vol. 28, no. 10. American Society
of Plant Biologists, pp. 2464–2477, 2016.
ista: Žádníková P, Wabnik KT, Abuzeineh A, Gallemí M, Van Der Straeten D, Smith
R, Inze D, Friml J, Prusinkiewicz P, Benková E. 2016. A model of differential
growth guided apical hook formation in plants. Plant Cell. 28(10), 2464–2477.
mla: Žádníková, Petra, et al. “A Model of Differential Growth Guided Apical Hook
Formation in Plants.” Plant Cell, vol. 28, no. 10, American Society of
Plant Biologists, 2016, pp. 2464–77, doi:10.1105/tpc.15.00569.
short: P. Žádníková, K.T. Wabnik, A. Abuzeineh, M. Gallemí, D. Van Der Straeten,
R. Smith, D. Inze, J. Friml, P. Prusinkiewicz, E. Benková, Plant Cell 28 (2016)
2464–2477.
date_created: 2018-12-11T11:50:26Z
date_published: 2016-10-01T00:00:00Z
date_updated: 2021-01-12T06:48:40Z
day: '01'
department:
- _id: EvBe
- _id: JiFr
doi: 10.1105/tpc.15.00569
ec_funded: 1
intvolume: ' 28'
issue: '10'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5134968/
month: '10'
oa: 1
oa_version: Submitted Version
page: 2464 - 2477
project:
- _id: 253FCA6A-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '207362'
name: Hormonal cross-talk in plant organogenesis
publication: Plant Cell
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '6205'
quality_controlled: '1'
scopus_import: 1
status: public
title: A model of differential growth guided apical hook formation in plants
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 28
year: '2016'
...
---
_id: '1212'
abstract:
- lang: eng
text: 'Plants adjust their growth according to gravity. Gravitropism involves gravity
perception, signal transduction, and asymmetric growth response, with organ bending
as a consequence [1]. Asymmetric growth results from the asymmetric distribution
of the plant-specific signaling molecule auxin [2] that is generated by lateral
transport, mediated in the hypocotyl predominantly by the auxin transporter PIN-FORMED3
(PIN3) [3–5]. Gravity stimulation polarizes PIN3 to the bottom sides of endodermal
cells, correlating with increased auxin accumulation in adjacent tissues at the
lower side of the stimulated organ, where auxin induces cell elongation and, hence,
organ bending. A curvature response allows the hypocotyl to resume straight growth
at a defined angle [6], implying that at some point auxin symmetry is restored
to prevent overbending. Here, we present initial insights into cellular and molecular
mechanisms that lead to the termination of the tropic response. We identified
an auxin feedback on PIN3 polarization as underlying mechanism that restores symmetry
of the PIN3-dependent auxin flow. Thus, two mechanistically distinct PIN3 polarization
events redirect auxin fluxes at different time points of the gravity response:
first, gravity-mediated redirection of PIN3-mediated auxin flow toward the lower
hypocotyl side, where auxin gradually accumulates and promotes growth, and later
PIN3 polarization to the opposite cell side, depleting this auxin maximum to end
the bending. Accordingly, genetic or pharmacological interference with the late
PIN3 polarization prevents termination of the response and leads to hypocotyl
overbending. This observation reveals a role of auxin feedback on PIN polarity
in the termination of the tropic response. © 2016 Elsevier Ltd'
acknowledgement: "We thank Dr. Jie Li (Key Laboratory of Plant Molecular Physiology,
Chinese Academy of Science, China) for the pPIN3::PIN3-GFP/DII::VENUS line and Martine
De Cock for help in preparing the manuscript. This work was supported by the European
Research Council (project ERC-2011-StG-20101109-PSDP), by the Czech Science Foundation
GAČR (GA13-40637S) to J.F., and by the Ministry of Education, Youth and Sports of
the Czech Republic under the project CEITEC 2020 (LQ1601) to H.S.R. H.R. is indebted
to the Agency for Innovation by Science and Technology (IWT) for a predoctoral fellowship.\r\n"
author:
- first_name: Hana
full_name: Rakusová, Hana
last_name: Rakusová
- first_name: Mohamad
full_name: Abbas, Mohamad
id: 47E8FC1C-F248-11E8-B48F-1D18A9856A87
last_name: Abbas
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
- first_name: Siyuan
full_name: Song, Siyuan
last_name: Song
- first_name: Hélène
full_name: Robert, Hélène
last_name: Robert
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Rakusová H, Abbas M, Han H, Song S, Robert H, Friml J. Termination of shoot
gravitropic responses by auxin feedback on PIN3 polarity. Current Biology.
2016;26(22):3026-3032. doi:10.1016/j.cub.2016.08.067
apa: Rakusová, H., Abbas, M., Han, H., Song, S., Robert, H., & Friml, J. (2016).
Termination of shoot gravitropic responses by auxin feedback on PIN3 polarity.
Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2016.08.067
chicago: Rakusová, Hana, Mohamad Abbas, Huibin Han, Siyuan Song, Hélène Robert,
and Jiří Friml. “Termination of Shoot Gravitropic Responses by Auxin Feedback
on PIN3 Polarity.” Current Biology. Cell Press, 2016. https://doi.org/10.1016/j.cub.2016.08.067.
ieee: H. Rakusová, M. Abbas, H. Han, S. Song, H. Robert, and J. Friml, “Termination
of shoot gravitropic responses by auxin feedback on PIN3 polarity,” Current
Biology, vol. 26, no. 22. Cell Press, pp. 3026–3032, 2016.
ista: Rakusová H, Abbas M, Han H, Song S, Robert H, Friml J. 2016. Termination of
shoot gravitropic responses by auxin feedback on PIN3 polarity. Current Biology.
26(22), 3026–3032.
mla: Rakusová, Hana, et al. “Termination of Shoot Gravitropic Responses by Auxin
Feedback on PIN3 Polarity.” Current Biology, vol. 26, no. 22, Cell Press,
2016, pp. 3026–32, doi:10.1016/j.cub.2016.08.067.
short: H. Rakusová, M. Abbas, H. Han, S. Song, H. Robert, J. Friml, Current Biology
26 (2016) 3026–3032.
date_created: 2018-12-11T11:50:44Z
date_published: 2016-11-21T00:00:00Z
date_updated: 2021-01-12T06:49:08Z
day: '21'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.1016/j.cub.2016.08.067
ec_funded: 1
file:
- access_level: open_access
checksum: 79ed2498185a027cf51a8f88100379e6
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:09:33Z
date_updated: 2020-07-14T12:44:39Z
file_id: '4757'
file_name: IST-2018-1008-v1+1_Rakusova_CurrBiol_2016_proof.pdf
file_size: 5391923
relation: main_file
file_date_updated: 2020-07-14T12:44:39Z
has_accepted_license: '1'
intvolume: ' 26'
issue: '22'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Submitted Version
page: 3026 - 3032
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Current Biology
publication_status: published
publisher: Cell Press
publist_id: '6138'
pubrep_id: '1008'
quality_controlled: '1'
scopus_import: 1
status: public
title: Termination of shoot gravitropic responses by auxin feedback on PIN3 polarity
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 26
year: '2016'
...
---
_id: '1221'
abstract:
- lang: eng
text: The Auxin Binding Protein 1 (ABP1) is one of the most studied proteins in
plants. Since decades ago, it has been the prime receptor candidate for the plant
hormone auxin with a plethora of described functions in auxin signaling and development.
The developmental importance of ABP1 has recently been questioned by identification
of Arabidopsis thaliana abp1 knock-out alleles that show no obvious phenotypes
under normal growth conditions. In this study, we examined the contradiction between
the normal growth and development of the abp1 knock-outs and the strong morphological
defects observed in three different ethanol-inducible abp1 knock-down mutants
( abp1-AS, SS12K, SS12S). By analyzing segregating populations of abp1 knock-out
vs. abp1 knock-down crosses we show that the strong morphological defects that
were believed to be the result of conditional down-regulation of ABP1 can be reproduced
also in the absence of the functional ABP1 protein. This data suggests that the
phenotypes in abp1 knock-down lines are due to the off-target effects and asks
for further reflections on the biological function of ABP1 or alternative explanations
for the missing phenotypic defects in the abp1 loss-of-function alleles.
acknowledgement: "This work was supported by ERC Independent Research grant (ERC-2011-StG-20101109-PSDP
to JF). JM internship was supported by the grant “Action Austria – Slovakia”. MG
was supported by the scholarship \"Stipendien der Stipendienstiftung der Republik
Österreich\". Work by EH and CPR were supported by ANR blanc ANR-14-CE11-0018. We
would like to thank Mark Estelle and Yunde Zhao for provid\r\n-\r\ning \r\nabp1-c1\r\n,
\r\nabp1-TD1 \r\nand \r\nabp1-WTc1 \r\nseeds. We thank Emeline \r\nHuault for technical
assistance."
article_number: '86'
article_processing_charge: No
article_type: original
author:
- first_name: Jaroslav
full_name: Michalko, Jaroslav
id: 483727CA-F248-11E8-B48F-1D18A9856A87
last_name: Michalko
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: Catherine
full_name: Perrot Rechenmann, Catherine
last_name: Perrot Rechenmann
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Michalko J, Glanc M, Perrot Rechenmann C, Friml J. Strong morphological defects
in conditional Arabidopsis abp1 knock-down mutants generated in absence of functional
ABP1 protein. F1000 Research . 2016;5. doi:10.12688/f1000research.7654.1
apa: Michalko, J., Glanc, M., Perrot Rechenmann, C., & Friml, J. (2016). Strong
morphological defects in conditional Arabidopsis abp1 knock-down mutants generated
in absence of functional ABP1 protein. F1000 Research . F1000 Research.
https://doi.org/10.12688/f1000research.7654.1
chicago: Michalko, Jaroslav, Matous Glanc, Catherine Perrot Rechenmann, and Jiří
Friml. “Strong Morphological Defects in Conditional Arabidopsis Abp1 Knock-down
Mutants Generated in Absence of Functional ABP1 Protein.” F1000 Research .
F1000 Research, 2016. https://doi.org/10.12688/f1000research.7654.1.
ieee: J. Michalko, M. Glanc, C. Perrot Rechenmann, and J. Friml, “Strong morphological
defects in conditional Arabidopsis abp1 knock-down mutants generated in absence
of functional ABP1 protein,” F1000 Research , vol. 5. F1000 Research, 2016.
ista: Michalko J, Glanc M, Perrot Rechenmann C, Friml J. 2016. Strong morphological
defects in conditional Arabidopsis abp1 knock-down mutants generated in absence
of functional ABP1 protein. F1000 Research . 5, 86.
mla: Michalko, Jaroslav, et al. “Strong Morphological Defects in Conditional Arabidopsis
Abp1 Knock-down Mutants Generated in Absence of Functional ABP1 Protein.” F1000
Research , vol. 5, 86, F1000 Research, 2016, doi:10.12688/f1000research.7654.1.
short: J. Michalko, M. Glanc, C. Perrot Rechenmann, J. Friml, F1000 Research 5
(2016).
date_created: 2018-12-11T11:50:47Z
date_published: 2016-01-20T00:00:00Z
date_updated: 2022-03-24T09:12:49Z
day: '20'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.12688/f1000research.7654.1
ec_funded: 1
file:
- access_level: open_access
checksum: c9e50bb6096a7ba4a832969935820f19
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:15:33Z
date_updated: 2020-07-14T12:44:39Z
file_id: '5154'
file_name: IST-2016-711-v1+1_770cf1e0-612f-4e85-a500-54b6349fbbab_7654_-_jaroslav_michalko.pdf
file_size: 2990459
relation: main_file
file_date_updated: 2020-07-14T12:44:39Z
has_accepted_license: '1'
intvolume: ' 5'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: 'F1000 Research '
publication_status: published
publisher: F1000 Research
publist_id: '6113'
pubrep_id: '711'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Strong morphological defects in conditional Arabidopsis abp1 knock-down mutants
generated in absence of functional ABP1 protein
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 5
year: '2016'
...
---
_id: '1238'
abstract:
- lang: eng
text: The dynamic localization of endosomal compartments labeled with targeted fluorescent
protein tags is routinely followed by time lapse fluorescence microscopy approaches
and single particle tracking algorithms. In this way trajectories of individual
endosomes can be mapped and linked to physiological processes as cell growth.
However, other aspects of dynamic behavior including endosomal interactions are
difficult to follow in this manner. Therefore, we characterized the localization
and dynamic properties of early and late endosomes throughout the entire course
of root hair formation by means of spinning disc time lapse imaging and post-acquisition
automated multitracking and quantitative analysis. Our results show differential
motile behavior of early and late endosomes and interactions of late endosomes
that may be specified to particular root hair domains. Detailed data analysis
revealed a particular transient interaction between late endosomes—termed herein
as dancing-endosomes—which is not concluding to vesicular fusion. Endosomes preferentially
located in the root hair tip interacted as dancing-endosomes and traveled short
distances during this interaction. Finally, sizes of early and late endosomes
were addressed by means of super-resolution structured illumination microscopy
(SIM) to corroborate measurements on the spinning disc. This is a first study
providing quantitative microscopic data on dynamic spatio-temporal interactions
of endosomes during root hair tip growth.
acknowledgement: "This work was supported by National Program for Sustainability I
(grant no. LO1204) provided by the Czech Ministry of Education and by Institutional
Fund of Palacký University Olomouc (GK and OŠ).\r\nWe thank Sabine Fischer for help
with the statistics."
article_number: '1262'
author:
- first_name: Daniel
full_name: Von Wangenheim, Daniel
id: 49E91952-F248-11E8-B48F-1D18A9856A87
last_name: Von Wangenheim
orcid: 0000-0002-6862-1247
- first_name: Amparo
full_name: Rosero, Amparo
last_name: Rosero
- first_name: George
full_name: Komis, George
last_name: Komis
- first_name: Olga
full_name: Šamajová, Olga
last_name: Šamajová
- first_name: Miroslav
full_name: Ovečka, Miroslav
last_name: Ovečka
- first_name: Boris
full_name: Voigt, Boris
last_name: Voigt
- first_name: Jozef
full_name: Šamaj, Jozef
last_name: Šamaj
citation:
ama: von Wangenheim D, Rosero A, Komis G, et al. Endosomal interactions during root
hair growth. Frontiers in Plant Science. 2016;6(JAN2016). doi:10.3389/fpls.2015.01262
apa: von Wangenheim, D., Rosero, A., Komis, G., Šamajová, O., Ovečka, M., Voigt,
B., & Šamaj, J. (2016). Endosomal interactions during root hair growth. Frontiers
in Plant Science. Frontiers Research Foundation. https://doi.org/10.3389/fpls.2015.01262
chicago: Wangenheim, Daniel von, Amparo Rosero, George Komis, Olga Šamajová, Miroslav
Ovečka, Boris Voigt, and Jozef Šamaj. “Endosomal Interactions during Root Hair
Growth.” Frontiers in Plant Science. Frontiers Research Foundation, 2016.
https://doi.org/10.3389/fpls.2015.01262.
ieee: D. von Wangenheim et al., “Endosomal interactions during root hair
growth,” Frontiers in Plant Science, vol. 6, no. JAN2016. Frontiers Research
Foundation, 2016.
ista: von Wangenheim D, Rosero A, Komis G, Šamajová O, Ovečka M, Voigt B, Šamaj
J. 2016. Endosomal interactions during root hair growth. Frontiers in Plant Science.
6(JAN2016), 1262.
mla: von Wangenheim, Daniel, et al. “Endosomal Interactions during Root Hair Growth.”
Frontiers in Plant Science, vol. 6, no. JAN2016, 1262, Frontiers Research
Foundation, 2016, doi:10.3389/fpls.2015.01262.
short: D. von Wangenheim, A. Rosero, G. Komis, O. Šamajová, M. Ovečka, B. Voigt,
J. Šamaj, Frontiers in Plant Science 6 (2016).
date_created: 2018-12-11T11:50:53Z
date_published: 2016-01-29T00:00:00Z
date_updated: 2021-01-12T06:49:18Z
day: '29'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.3389/fpls.2015.01262
file:
- access_level: open_access
checksum: 3127eab844d53564bf47e2b6b42f1ca0
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:09:36Z
date_updated: 2020-07-14T12:44:41Z
file_id: '4760'
file_name: IST-2016-710-v1+1_fpls-06-01262.pdf
file_size: 1640550
relation: main_file
file_date_updated: 2020-07-14T12:44:41Z
has_accepted_license: '1'
intvolume: ' 6'
issue: JAN2016
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
publication: Frontiers in Plant Science
publication_status: published
publisher: Frontiers Research Foundation
publist_id: '6094'
pubrep_id: '710'
quality_controlled: '1'
scopus_import: 1
status: public
title: Endosomal interactions during root hair growth
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 6
year: '2016'
...
---
_id: '1247'
abstract:
- lang: eng
text: The shaping of organs in plants depends on the intercellular flow of the phytohormone
auxin, of which the directional signaling is determined by the polar subcellular
localization of PIN-FORMED (PIN) auxin transport proteins. Phosphorylation dynamics
of PIN proteins are affected by the protein phosphatase 2A (PP2A) and the PINOID
kinase, which act antagonistically to mediate their apical-basal polar delivery.
Here, we identified the ROTUNDA3 (RON3) protein as a regulator of the PP2A phosphatase
activity in Arabidopsis thaliana. The RON3 gene was map-based cloned starting
from the ron3-1 leaf mutant and found to be a unique, plant-specific gene coding
for a protein with high and dispersed proline content. The ron3-1 and ron3-2 mutant
phenotypes [i.e., reduced apical dominance, primary root length, lateral root
emergence, and growth; increased ectopic stages II, IV, and V lateral root primordia;
decreased auxin maxima in indole-3-acetic acid (IAA)-treated root apical meristems;
hypergravitropic root growth and response; increased IAA levels in shoot apices;
and reduced auxin accumulation in root meristems] support a role for RON3 in auxin
biology. The affinity-purified PP2A complex with RON3 as bait suggested that RON3
might act in PIN transporter trafficking. Indeed, pharmacological interference
with vesicle trafficking processes revealed that single ron3-2 and double ron3-2
rcn1 mutants have altered PIN polarity and endocytosis in specific cells. Our
data indicate that RON3 contributes to auxin-mediated development by playing a
role in PIN recycling and polarity establishment through regulation of the PP2A
complex activity.
acknowledgement: "This work was supported by the Ghent University Special Research
Fund (M.K.), the European Research Council (Project ERC-2011-StG-20101109-PSDP)
(to J.F.), and the Körber European Science Foun-\r\ndation (J.F.). S.D.G. is indebted
to the Agency for Science and Technology for\r\na predoctoral fellowship."
author:
- first_name: Michael
full_name: Karampelias, Michael
last_name: Karampelias
- first_name: Pia
full_name: Neyt, Pia
last_name: Neyt
- first_name: Steven
full_name: De Groeve, Steven
last_name: De Groeve
- first_name: Stijn
full_name: Aesaert, Stijn
last_name: Aesaert
- first_name: Griet
full_name: Coussens, Griet
last_name: Coussens
- first_name: Jakub
full_name: Rolčík, Jakub
last_name: Rolčík
- first_name: Leonardo
full_name: Bruno, Leonardo
last_name: Bruno
- first_name: Nancy
full_name: De Winne, Nancy
last_name: De Winne
- first_name: Annemie
full_name: Van Minnebruggen, Annemie
last_name: Van Minnebruggen
- first_name: Marc
full_name: Van Montagu, Marc
last_name: Van Montagu
- first_name: Maria
full_name: Ponce, Maria
last_name: Ponce
- first_name: José
full_name: Micol, José
last_name: Micol
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Geert
full_name: De Jaeger, Geert
last_name: De Jaeger
- first_name: Mieke
full_name: Van Lijsebettens, Mieke
last_name: Van Lijsebettens
citation:
ama: Karampelias M, Neyt P, De Groeve S, et al. ROTUNDA3 function in plant development
by phosphatase 2A-mediated regulation of auxin transporter recycling. PNAS.
2016;113(10):2768-2773. doi:10.1073/pnas.1501343112
apa: Karampelias, M., Neyt, P., De Groeve, S., Aesaert, S., Coussens, G., Rolčík,
J., … Van Lijsebettens, M. (2016). ROTUNDA3 function in plant development by phosphatase
2A-mediated regulation of auxin transporter recycling. PNAS. National Academy
of Sciences. https://doi.org/10.1073/pnas.1501343112
chicago: Karampelias, Michael, Pia Neyt, Steven De Groeve, Stijn Aesaert, Griet
Coussens, Jakub Rolčík, Leonardo Bruno, et al. “ROTUNDA3 Function in Plant Development
by Phosphatase 2A-Mediated Regulation of Auxin Transporter Recycling.” PNAS.
National Academy of Sciences, 2016. https://doi.org/10.1073/pnas.1501343112.
ieee: M. Karampelias et al., “ROTUNDA3 function in plant development by phosphatase
2A-mediated regulation of auxin transporter recycling,” PNAS, vol. 113,
no. 10. National Academy of Sciences, pp. 2768–2773, 2016.
ista: Karampelias M, Neyt P, De Groeve S, Aesaert S, Coussens G, Rolčík J, Bruno
L, De Winne N, Van Minnebruggen A, Van Montagu M, Ponce M, Micol J, Friml J, De
Jaeger G, Van Lijsebettens M. 2016. ROTUNDA3 function in plant development by
phosphatase 2A-mediated regulation of auxin transporter recycling. PNAS. 113(10),
2768–2773.
mla: Karampelias, Michael, et al. “ROTUNDA3 Function in Plant Development by Phosphatase
2A-Mediated Regulation of Auxin Transporter Recycling.” PNAS, vol. 113,
no. 10, National Academy of Sciences, 2016, pp. 2768–73, doi:10.1073/pnas.1501343112.
short: M. Karampelias, P. Neyt, S. De Groeve, S. Aesaert, G. Coussens, J. Rolčík,
L. Bruno, N. De Winne, A. Van Minnebruggen, M. Van Montagu, M. Ponce, J. Micol,
J. Friml, G. De Jaeger, M. Van Lijsebettens, PNAS 113 (2016) 2768–2773.
date_created: 2018-12-11T11:50:56Z
date_published: 2016-03-08T00:00:00Z
date_updated: 2021-01-12T06:49:22Z
day: '08'
department:
- _id: JiFr
doi: 10.1073/pnas.1501343112
ec_funded: 1
intvolume: ' 113'
issue: '10'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4791031/
month: '03'
oa: 1
oa_version: Submitted Version
page: 2768 - 2773
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '6081'
quality_controlled: '1'
scopus_import: 1
status: public
title: ROTUNDA3 function in plant development by phosphatase 2A-mediated regulation
of auxin transporter recycling
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 113
year: '2016'
...
---
_id: '1251'
abstract:
- lang: eng
text: Plant growth and architecture is regulated by the polar distribution of the
hormone auxin. Polarity and flexibility of this process is provided by constant
cycling of auxin transporter vesicles along actin filaments, coordinated by a
positive auxinactin feedback loop. Both polar auxin transport and vesicle cycling
are inhibited by synthetic auxin transport inhibitors, such as 1-Nnaphthylphthalamic
acid (NPA), counteracting the effect of auxin; however, underlying targets and
mechanisms are unclear. Using NMR, we map the NPA binding surface on the Arabidopsis
thaliana ABCB chaperone TWISTED DWARF1 (TWD1).We identify ACTIN7 as a relevant,
although likely indirect, TWD1 interactor, and show TWD1-dependent regulation
of actin filament organization and dynamics and that TWD1 is required for NPA-mediated
actin cytoskeleton remodeling. The TWD1-ACTIN7 axis controls plasma membrane presence
of efflux transporters, and as a consequence act7 and twd1 share developmental
and physiological phenotypes indicative of defects in auxin transport. These can
be phenocopied by NPA treatment or by chemical actin (de)stabilization. We provide
evidence that TWD1 determines downstreamlocations of auxin efflux transporters
by adjusting actin filament debundling and dynamizing processes and mediating
NPA action on the latter. This function appears to be evolutionary conserved since
TWD1 expression in budding yeast alters actin polarization and cell polarity and
provides NPA sensitivity.
acknowledgement: ' This work was supported by grants from the European Social Fund
(CZ.1.07/2.3.00/20.0043), the Czech Science Foundation GAČR (GA13-40637S) to J.F.
and M.Z., the Ministry of Education, Youth, and Sports of the Czech Republic under
the project CEITEC 2020 (LQ1601) to M.Z., the Ministry for Higher Education and
Research of Luxembourg (REC-LOCM-20140703) to C.T., the Partial Funding Program
for Short Stays Abroad of CONICET Argentina (to N.I.B.), Swiss National Funds, the
Pool de Recherche of the University of Fribourg, and the Novartis Foundation (all
to M.G.). '
author:
- first_name: Jinsheng
full_name: Zhu, Jinsheng
last_name: Zhu
- first_name: Aurélien
full_name: Bailly, Aurélien
last_name: Bailly
- first_name: Marta
full_name: Zwiewka, Marta
last_name: Zwiewka
- first_name: Valpuri
full_name: Sovero, Valpuri
last_name: Sovero
- first_name: Martin
full_name: Di Donato, Martin
last_name: Di Donato
- first_name: Pei
full_name: Ge, Pei
last_name: Ge
- first_name: Jacqueline
full_name: Oehri, Jacqueline
last_name: Oehri
- first_name: Bibek
full_name: Aryal, Bibek
last_name: Aryal
- first_name: Pengchao
full_name: Hao, Pengchao
last_name: Hao
- first_name: Miriam
full_name: Linnert, Miriam
last_name: Linnert
- first_name: Noelia
full_name: Burgardt, Noelia
last_name: Burgardt
- first_name: Christian
full_name: Lücke, Christian
last_name: Lücke
- first_name: Matthias
full_name: Weiwad, Matthias
last_name: Weiwad
- first_name: Max
full_name: Michel, Max
last_name: Michel
- first_name: Oliver
full_name: Weiergräber, Oliver
last_name: Weiergräber
- first_name: Stephan
full_name: Pollmann, Stephan
last_name: Pollmann
- first_name: Elisa
full_name: Azzarello, Elisa
last_name: Azzarello
- first_name: Stefano
full_name: Mancuso, Stefano
last_name: Mancuso
- first_name: Noel
full_name: Ferro, Noel
last_name: Ferro
- first_name: Yoichiro
full_name: Fukao, Yoichiro
last_name: Fukao
- first_name: Céline
full_name: Hoffmann, Céline
last_name: Hoffmann
- first_name: Roland
full_name: Wedlich Söldner, Roland
last_name: Wedlich Söldner
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Clément
full_name: Thomas, Clément
last_name: Thomas
- first_name: Markus
full_name: Geisler, Markus
last_name: Geisler
citation:
ama: Zhu J, Bailly A, Zwiewka M, et al. TWISTED DWARF1 mediates the action of auxin
transport inhibitors on actin cytoskeleton dynamics. Plant Cell. 2016;28(4):930-948.
doi:10.1105/tpc.15.00726
apa: Zhu, J., Bailly, A., Zwiewka, M., Sovero, V., Di Donato, M., Ge, P., … Geisler,
M. (2016). TWISTED DWARF1 mediates the action of auxin transport inhibitors on
actin cytoskeleton dynamics. Plant Cell. American Society of Plant Biologists.
https://doi.org/10.1105/tpc.15.00726
chicago: Zhu, Jinsheng, Aurélien Bailly, Marta Zwiewka, Valpuri Sovero, Martin Di
Donato, Pei Ge, Jacqueline Oehri, et al. “TWISTED DWARF1 Mediates the Action of
Auxin Transport Inhibitors on Actin Cytoskeleton Dynamics.” Plant Cell.
American Society of Plant Biologists, 2016. https://doi.org/10.1105/tpc.15.00726.
ieee: J. Zhu et al., “TWISTED DWARF1 mediates the action of auxin transport
inhibitors on actin cytoskeleton dynamics,” Plant Cell, vol. 28, no. 4.
American Society of Plant Biologists, pp. 930–948, 2016.
ista: Zhu J, Bailly A, Zwiewka M, Sovero V, Di Donato M, Ge P, Oehri J, Aryal B,
Hao P, Linnert M, Burgardt N, Lücke C, Weiwad M, Michel M, Weiergräber O, Pollmann
S, Azzarello E, Mancuso S, Ferro N, Fukao Y, Hoffmann C, Wedlich Söldner R, Friml
J, Thomas C, Geisler M. 2016. TWISTED DWARF1 mediates the action of auxin transport
inhibitors on actin cytoskeleton dynamics. Plant Cell. 28(4), 930–948.
mla: Zhu, Jinsheng, et al. “TWISTED DWARF1 Mediates the Action of Auxin Transport
Inhibitors on Actin Cytoskeleton Dynamics.” Plant Cell, vol. 28, no. 4,
American Society of Plant Biologists, 2016, pp. 930–48, doi:10.1105/tpc.15.00726.
short: J. Zhu, A. Bailly, M. Zwiewka, V. Sovero, M. Di Donato, P. Ge, J. Oehri,
B. Aryal, P. Hao, M. Linnert, N. Burgardt, C. Lücke, M. Weiwad, M. Michel, O.
Weiergräber, S. Pollmann, E. Azzarello, S. Mancuso, N. Ferro, Y. Fukao, C. Hoffmann,
R. Wedlich Söldner, J. Friml, C. Thomas, M. Geisler, Plant Cell 28 (2016) 930–948.
date_created: 2018-12-11T11:50:57Z
date_published: 2016-04-01T00:00:00Z
date_updated: 2021-01-12T06:49:24Z
day: '01'
department:
- _id: JiFr
doi: 10.1105/tpc.15.00726
intvolume: ' 28'
issue: '4'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4863381/
month: '04'
oa: 1
oa_version: Submitted Version
page: 930 - 948
publication: Plant Cell
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '6078'
quality_controlled: '1'
scopus_import: 1
status: public
title: TWISTED DWARF1 mediates the action of auxin transport inhibitors on actin cytoskeleton
dynamics
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 28
year: '2016'
...
---
_id: '1264'
abstract:
- lang: eng
text: n contrast with the wealth of recent reports about the function of μ-adaptins
and clathrin adaptor protein (AP) complexes, there is very little information
about the motifs that determine the sorting of membrane proteins within clathrin-coated
vesicles in plants. Here, we investigated putative sorting signals in the large
cytosolic loop of the Arabidopsis (Arabidopsis thaliana) PIN-FORMED1 (PIN1) auxin
transporter, which are involved in binding μ-adaptins and thus in PIN1 trafficking
and localization. We found that Phe-165 and Tyr-280, Tyr-328, and Tyr-394 are
involved in the binding of different μ-adaptins in vitro. However, only Phe-165,
which binds μA(μ2)- and μD(μ3)-adaptin, was found to be essential for PIN1 trafficking
and localization in vivo. The PIN1:GFP-F165A mutant showed reduced endocytosis
but also localized to intracellular structures containing several layers of membranes
and endoplasmic reticulum (ER) markers, suggesting that they correspond to ER
or ER-derived membranes. While PIN1:GFP localized normally in a μA (μ2)-adaptin
mutant, it accumulated in big intracellular structures containing LysoTracker
in a μD (μ3)-adaptin mutant, consistent with previous results obtained with mutants
of other subunits of the AP-3 complex. Our data suggest that Phe-165, through
the binding of μA (μ2)- and μD (μ3)-adaptin, is important for PIN1 endocytosis
and for PIN1 trafficking along the secretory pathway, respectively.
acknowledgement: "We thank Dr. R. Offringa (Leiden University) for providing the GST-\r\nPIN-CL
construct; Sandra Richter and Gerd Jurgens (University of Tübin-\r\ngen) for providing
the estradiol-inducible PIN1-RFP construct and the\r\ngnl1 mutant expressing BFA-sensitive
GNL1; F.J. Santonja (University of Valencia)\r\nfor help with the statistical analysis;
Jurgen Kleine-Vehn, Elke Barbez, and\r\nEva Benkova for helpful discussions; the
Salk Institute Genomic Analysis\r\nLaboratory for providing the sequence-indexed
Arabidopsis T-DNA in-\r\nsertion mutants; and the greenhouse section and the microscopy
section\r\nof SCSIE (University of Valencia) and Pilar Selvi for excellent technical\r\nassistance."
author:
- first_name: Gloria
full_name: Sancho Andrés, Gloria
last_name: Sancho Andrés
- first_name: Esther
full_name: Soriano Ortega, Esther
last_name: Soriano Ortega
- first_name: Caiji
full_name: Gao, Caiji
last_name: Gao
- first_name: Joan
full_name: Bernabé Orts, Joan
last_name: Bernabé Orts
- first_name: Madhumitha
full_name: Narasimhan, Madhumitha
id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
last_name: Narasimhan
orcid: 0000-0002-8600-0671
- first_name: Anna
full_name: Müller, Anna
id: 420AB15A-F248-11E8-B48F-1D18A9856A87
last_name: Müller
- first_name: Ricardo
full_name: Tejos, Ricardo
last_name: Tejos
- first_name: Liwen
full_name: Jiang, Liwen
last_name: Jiang
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Fernando
full_name: Aniento, Fernando
last_name: Aniento
- first_name: Maria
full_name: Marcote, Maria
last_name: Marcote
citation:
ama: Sancho Andrés G, Soriano Ortega E, Gao C, et al. Sorting motifs involved in
the trafficking and localization of the PIN1 auxin efflux carrier. Plant Physiology.
2016;171(3):1965-1982. doi:10.1104/pp.16.00373
apa: Sancho Andrés, G., Soriano Ortega, E., Gao, C., Bernabé Orts, J., Narasimhan,
M., Müller, A., … Marcote, M. (2016). Sorting motifs involved in the trafficking
and localization of the PIN1 auxin efflux carrier. Plant Physiology. American
Society of Plant Biologists. https://doi.org/10.1104/pp.16.00373
chicago: Sancho Andrés, Gloria, Esther Soriano Ortega, Caiji Gao, Joan Bernabé Orts,
Madhumitha Narasimhan, Anna Müller, Ricardo Tejos, et al. “Sorting Motifs Involved
in the Trafficking and Localization of the PIN1 Auxin Efflux Carrier.” Plant
Physiology. American Society of Plant Biologists, 2016. https://doi.org/10.1104/pp.16.00373.
ieee: G. Sancho Andrés et al., “Sorting motifs involved in the trafficking
and localization of the PIN1 auxin efflux carrier,” Plant Physiology, vol.
171, no. 3. American Society of Plant Biologists, pp. 1965–1982, 2016.
ista: Sancho Andrés G, Soriano Ortega E, Gao C, Bernabé Orts J, Narasimhan M, Müller
A, Tejos R, Jiang L, Friml J, Aniento F, Marcote M. 2016. Sorting motifs involved
in the trafficking and localization of the PIN1 auxin efflux carrier. Plant Physiology.
171(3), 1965–1982.
mla: Sancho Andrés, Gloria, et al. “Sorting Motifs Involved in the Trafficking and
Localization of the PIN1 Auxin Efflux Carrier.” Plant Physiology, vol.
171, no. 3, American Society of Plant Biologists, 2016, pp. 1965–82, doi:10.1104/pp.16.00373.
short: G. Sancho Andrés, E. Soriano Ortega, C. Gao, J. Bernabé Orts, M. Narasimhan,
A. Müller, R. Tejos, L. Jiang, J. Friml, F. Aniento, M. Marcote, Plant Physiology
171 (2016) 1965–1982.
date_created: 2018-12-11T11:51:01Z
date_published: 2016-07-01T00:00:00Z
date_updated: 2021-01-12T06:49:29Z
day: '01'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1104/pp.16.00373
ec_funded: 1
intvolume: ' 171'
issue: '3'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4936568/
month: '07'
oa: 1
oa_version: Submitted Version
page: 1965 - 1982
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Plant Physiology
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '6059'
quality_controlled: '1'
scopus_import: 1
status: public
title: Sorting motifs involved in the trafficking and localization of the PIN1 auxin
efflux carrier
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 171
year: '2016'
...
---
_id: '1277'
abstract:
- lang: eng
text: "The Arabidopsis thaliana endogenous elicitor peptides (AtPeps) are released
into the apoplast after cellular damage caused by pathogens or wounding to induce
innate immunity by direct binding to the membrane-localized leucine-rich repeat
receptor kinases, PEP RECEPTOR1 (PEPR1) and PEPR2. Although the PEPR-mediated
signaling components and responses have been studied extensively, the contributions
of the subcellular localization and dynamics of the active PEPRs remain largely
unknown. We used live-cell imaging of the fluorescently labeled and bioactive
pep1 to visualize the intracellular behavior of the PEPRs in the Arabidopsis root
meristem. We found that AtPep1 decorated the plasma membrane (PM) in a receptor-dependent
manner and cointernalized with PEPRs. Trafficking of the AtPep1-PEPR1 complexes
to the vacuole required neither the trans-Golgi network/early endosome (TGN/EE)-localized
vacuolar H+ -ATPase activity nor the function of the brefeldin A-sensitive ADP-ribosylation
factor-guanine exchange factors (ARF-GEFs). In addition, AtPep1 and different
TGN/EE markers colocalized only rarely, implying that the intracellular route
of this receptor-ligand pair is largely independent of the TGN/EE. Inducible overexpression
of the Arabidopsis clathrin coat disassembly factor, Auxilin2, which inhibits
clathrin-mediated endocytosis (CME), impaired the AtPep1-PEPR1 internalization
and compromised AtPep1-mediated responses. Our results show that clathrin function
at the PM is required to induce plant defense responses, likely through CME of
cell surface-located signaling components.\r\n"
acknowledgement: "F.A.O.-M. was supported by special\r\nresearch funding from the
Flemish Government for a joint doctorate fellowship\r\nat Ghent University, and
funding from the Student Program\r\n–\r\nGraduate Studies\r\nPlan Program from the
Coordination for the Improvement of Higher Educa-\r\ntion Personnel, Brazil, for
a doctorate fellowship at the University of São Paulo.\r\nX.Z. and Q.L. are indebted
to the China Science Council and G.P.d.O. to the\r\n“\r\nCiência sem Fronteiras\r\n”\r\nfor
predoctoral fellowships. R.K. and Y.L. have re-\r\nceived postdoctoral fellowships
from the Belgian Science Policy Office. This\r\nresearch was supported by Flanders
Research Foundation Grant G008416N\r\n(to E.R.) and by the São Paulo Research Foundation
and the National Council\r\nfor Scientific and Technological Development (CNPq)
(D.S.d.M.). D.S.d.M. is a\r\nresearch fellow of CNPq.\r\nWe thank D. Van Damme,
E. Mylle, M. Castro Silva-Filho,\r\nand J. Goeman for providing usefu\r\nl advice
and technical assistance;\r\nI. Hara-Nishimura, J. Lin, G. Jürgens, M. A. Johnson,
and P. Bozhkov for sharing\r\npublished materials; and M. Nowack and M. Fendrych
for kindly donating the\r\npUBQ10::ATG8-YFP\r\n-expressing marker line."
author:
- first_name: Fausto
full_name: Ortiz Morea, Fausto
last_name: Ortiz Morea
- first_name: Daniel
full_name: Savatin, Daniel
last_name: Savatin
- first_name: Wim
full_name: Dejonghe, Wim
last_name: Dejonghe
- first_name: Rahul
full_name: Kumar, Rahul
last_name: Kumar
- first_name: Yu
full_name: Luo, Yu
last_name: Luo
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Jos
full_name: Van Begin, Jos
last_name: Van Begin
- first_name: Keini
full_name: Dressano, Keini
last_name: Dressano
- first_name: Guilherme
full_name: De Oliveira, Guilherme
last_name: De Oliveira
- first_name: Xiuyang
full_name: Zhao, Xiuyang
last_name: Zhao
- first_name: Qing
full_name: Lu, Qing
last_name: Lu
- first_name: Annemieke
full_name: Madder, Annemieke
last_name: Madder
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Daniel
full_name: De Moura, Daniel
last_name: De Moura
- first_name: Eugenia
full_name: Russinova, Eugenia
last_name: Russinova
citation:
ama: Ortiz Morea F, Savatin D, Dejonghe W, et al. Danger-associated peptide signaling
in Arabidopsis requires clathrin. PNAS. 2016;113(39):11028-11033. doi:10.1073/pnas.1605588113
apa: Ortiz Morea, F., Savatin, D., Dejonghe, W., Kumar, R., Luo, Y., Adamowski,
M., … Russinova, E. (2016). Danger-associated peptide signaling in Arabidopsis
requires clathrin. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1605588113
chicago: Ortiz Morea, Fausto, Daniel Savatin, Wim Dejonghe, Rahul Kumar, Yu Luo,
Maciek Adamowski, Jos Van Begin, et al. “Danger-Associated Peptide Signaling in
Arabidopsis Requires Clathrin.” PNAS. National Academy of Sciences, 2016.
https://doi.org/10.1073/pnas.1605588113.
ieee: F. Ortiz Morea et al., “Danger-associated peptide signaling in Arabidopsis
requires clathrin,” PNAS, vol. 113, no. 39. National Academy of Sciences,
pp. 11028–11033, 2016.
ista: Ortiz Morea F, Savatin D, Dejonghe W, Kumar R, Luo Y, Adamowski M, Van Begin
J, Dressano K, De Oliveira G, Zhao X, Lu Q, Madder A, Friml J, De Moura D, Russinova
E. 2016. Danger-associated peptide signaling in Arabidopsis requires clathrin.
PNAS. 113(39), 11028–11033.
mla: Ortiz Morea, Fausto, et al. “Danger-Associated Peptide Signaling in Arabidopsis
Requires Clathrin.” PNAS, vol. 113, no. 39, National Academy of Sciences,
2016, pp. 11028–33, doi:10.1073/pnas.1605588113.
short: F. Ortiz Morea, D. Savatin, W. Dejonghe, R. Kumar, Y. Luo, M. Adamowski,
J. Van Begin, K. Dressano, G. De Oliveira, X. Zhao, Q. Lu, A. Madder, J. Friml,
D. De Moura, E. Russinova, PNAS 113 (2016) 11028–11033.
date_created: 2018-12-11T11:51:06Z
date_published: 2016-09-27T00:00:00Z
date_updated: 2021-01-12T06:49:34Z
day: '27'
department:
- _id: JiFr
doi: 10.1073/pnas.1605588113
intvolume: ' 113'
issue: '39'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5047203/
month: '09'
oa: 1
oa_version: Preprint
page: 11028 - 11033
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '6039'
quality_controlled: '1'
scopus_import: 1
status: public
title: Danger-associated peptide signaling in Arabidopsis requires clathrin
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 113
year: '2016'
...
---
_id: '1344'
abstract:
- lang: eng
text: Despite being composed of immobile cells, plants reorient along directional
stimuli. The hormone auxin is redistributed in stimulated organs leading to differential
growth and bending. Auxin application triggers rapid cell wall acidification and
elongation of aerial organs of plants, but the molecular players mediating these
effects are still controversial. Here we use genetically-encoded pH and auxin
signaling sensors, pharmacological and genetic manipulations available for Arabidopsis
etiolated hypocotyls to clarify how auxin is perceived and the downstream growth
executed. We show that auxin-induced acidification occurs by local activation
of H+-ATPases, which in the context of gravity response is restricted to the lower
organ side. This auxin-stimulated acidification and growth require TIR1/AFB-Aux/IAA
nuclear auxin perception. In addition, auxin-induced gene transcription and specifically
SAUR proteins are crucial downstream mediators of this growth. Our study provides
strong experimental support for the acid growth theory and clarified the contribution
of the upstream auxin perception mechanisms.
acknowledgement: "The authors express their gratitude to Veronika Bierbaum, Robert
Hauschild for help with MATLAB,\r\nDaniel von Wangenheim for the gravitropism assay.
We are thankful to Bill Gray, Mark Estelle,\r\nMichael Prigge, Ottoline Leyser,
Claudia Oecking for sharing the seeds with us. We thank Katelyn\r\nSageman-Furnas
and the members of the Friml lab for critical reading of the manuscript. The\r\nresearch
leading to these results has received funding from the People Programme (Marie Curie\r\nActions)
of the European Union’s Seventh Framework Programme (FP7/2007-2013) under REA grant\r\nagreement
n° 291734. This work was also supported by the European Research Council (project\r\nERC-2011-StG-20101109-PSDP)."
article_number: e19048
author:
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: Jeffrey
full_name: Leung, Jeffrey
last_name: Leung
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Fendrych M, Leung J, Friml J. TIR1 AFB Aux IAA auxin perception mediates rapid
cell wall acidification and growth of Arabidopsis hypocotyls. eLife. 2016;5.
doi:10.7554/eLife.19048
apa: Fendrych, M., Leung, J., & Friml, J. (2016). TIR1 AFB Aux IAA auxin perception
mediates rapid cell wall acidification and growth of Arabidopsis hypocotyls. ELife.
eLife Sciences Publications. https://doi.org/10.7554/eLife.19048
chicago: Fendrych, Matyas, Jeffrey Leung, and Jiří Friml. “TIR1 AFB Aux IAA Auxin
Perception Mediates Rapid Cell Wall Acidification and Growth of Arabidopsis Hypocotyls.”
ELife. eLife Sciences Publications, 2016. https://doi.org/10.7554/eLife.19048.
ieee: M. Fendrych, J. Leung, and J. Friml, “TIR1 AFB Aux IAA auxin perception mediates
rapid cell wall acidification and growth of Arabidopsis hypocotyls,” eLife,
vol. 5. eLife Sciences Publications, 2016.
ista: Fendrych M, Leung J, Friml J. 2016. TIR1 AFB Aux IAA auxin perception mediates
rapid cell wall acidification and growth of Arabidopsis hypocotyls. eLife. 5,
e19048.
mla: Fendrych, Matyas, et al. “TIR1 AFB Aux IAA Auxin Perception Mediates Rapid
Cell Wall Acidification and Growth of Arabidopsis Hypocotyls.” ELife, vol.
5, e19048, eLife Sciences Publications, 2016, doi:10.7554/eLife.19048.
short: M. Fendrych, J. Leung, J. Friml, ELife 5 (2016).
date_created: 2018-12-11T11:51:29Z
date_published: 2016-09-14T00:00:00Z
date_updated: 2021-01-12T06:50:01Z
day: '14'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.7554/eLife.19048
ec_funded: 1
file:
- access_level: open_access
checksum: 9209541fbba00f24daad21a5d568540d
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:09:24Z
date_updated: 2020-07-14T12:44:45Z
file_id: '4748'
file_name: IST-2016-693-v1+1_e19048-download.pdf
file_size: 5666343
relation: main_file
file_date_updated: 2020-07-14T12:44:45Z
has_accepted_license: '1'
intvolume: ' 5'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
project:
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: eLife
publication_status: published
publisher: eLife Sciences Publications
publist_id: '5908'
pubrep_id: '654'
quality_controlled: '1'
scopus_import: 1
status: public
title: TIR1 AFB Aux IAA auxin perception mediates rapid cell wall acidification and
growth of Arabidopsis hypocotyls
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 5
year: '2016'
...
---
_id: '1345'
abstract:
- lang: eng
text: The electrostatic charge at the inner surface of the plasma membrane is strongly
negative in higher organisms. A new study shows that phosphatidylinositol-4-phosphate
plays a critical role in establishing plasma membrane surface charge in Arabidopsis,
which regulates the correct localization of signalling components.
article_number: '16102'
author:
- first_name: Gergely
full_name: Molnar, Gergely
id: 34F1AF46-F248-11E8-B48F-1D18A9856A87
last_name: Molnar
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Molnar G, Fendrych M, Friml J. Plasma membrane: Negative attraction. Nature
Plants. 2016;2. doi:10.1038/nplants.2016.102'
apa: 'Molnar, G., Fendrych, M., & Friml, J. (2016). Plasma membrane: Negative
attraction. Nature Plants. Nature Publishing Group. https://doi.org/10.1038/nplants.2016.102'
chicago: 'Molnar, Gergely, Matyas Fendrych, and Jiří Friml. “Plasma Membrane: Negative
Attraction.” Nature Plants. Nature Publishing Group, 2016. https://doi.org/10.1038/nplants.2016.102.'
ieee: 'G. Molnar, M. Fendrych, and J. Friml, “Plasma membrane: Negative attraction,”
Nature Plants, vol. 2. Nature Publishing Group, 2016.'
ista: 'Molnar G, Fendrych M, Friml J. 2016. Plasma membrane: Negative attraction.
Nature Plants. 2, 16102.'
mla: 'Molnar, Gergely, et al. “Plasma Membrane: Negative Attraction.” Nature
Plants, vol. 2, 16102, Nature Publishing Group, 2016, doi:10.1038/nplants.2016.102.'
short: G. Molnar, M. Fendrych, J. Friml, Nature Plants 2 (2016).
date_created: 2018-12-11T11:51:30Z
date_published: 2016-07-01T00:00:00Z
date_updated: 2021-01-12T06:50:02Z
day: '01'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.1038/nplants.2016.102
file:
- access_level: open_access
checksum: 9ba65f558563b287f875f48fa9f30fb2
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:12:36Z
date_updated: 2020-07-14T12:44:45Z
file_id: '4954'
file_name: IST-2018-1007-v1+1_Molnar_NatPlants_2016.pdf
file_size: 127781
relation: main_file
- access_level: open_access
checksum: 550d252be808d8ca2b43e83dddb4212f
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:12:37Z
date_updated: 2020-07-14T12:44:45Z
file_id: '4955'
file_name: IST-2018-1007-v1+2_Molnar_NatPlants_2016_editor_statement.pdf
file_size: 430556
relation: main_file
file_date_updated: 2020-07-14T12:44:45Z
has_accepted_license: '1'
intvolume: ' 2'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
publication: Nature Plants
publication_status: published
publisher: Nature Publishing Group
publist_id: '5907'
pubrep_id: '1007'
quality_controlled: '1'
scopus_import: 1
status: public
title: 'Plasma membrane: Negative attraction'
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 2
year: '2016'
...
---
_id: '1372'
abstract:
- lang: eng
text: Redirection of intercellular auxin fluxes via relocalization of the PIN-FORMED
3 (PIN3) and PIN7 auxin efflux carriers has been suggested to be necessary for
the root gravitropic response. Cytokinins have also been proposed to play a role
in controlling root gravitropism, but conclusive evidence is lacking. We present
a detailed study of the dynamics of root bending early after gravistimulation,
which revealed a delayed gravitropic response in transgenic lines with depleted
endogenous cytokinins (Pro35S:AtCKX) and cytokinin signaling mutants. Pro35S:AtCKX
lines, as well as a cytokinin receptor mutant ahk3, showed aberrations in the
auxin response distribution in columella cells consistent with defects in the
auxin transport machinery. Using in vivo real-time imaging of PIN3-GFP and PIN7-GFP
in AtCKX3 overexpression and ahk3 backgrounds, we observed wild-type-like relocalization
of PIN proteins in the columella early after gravistimulation, with gravity-induced
relocalization of PIN7 faster than that of PIN3. Nonetheless, the cellular distribution
of PIN3 and PIN7 and expression of PIN7 and the auxin influx carrier AUX1 was
affected in AtCKX overexpression lines. Based on the retained cytokinin sensitivity
in pin3 pin4 pin7 mutant, we propose the AUX1-mediated auxin transport rather
than columella-located PIN proteins as a target of endogenous cytokinins in the
control of root gravitropism.
acknowledgement: 'Funded by Ministry of Education, Youth and Sports Czech Republic.
Grant Numbers: CEITEC 2020, LQ1601, LO1204, LH14104 and The European Research Council.
Grant Number: ERC-2011-StG-20101109-PSDP and The Czech Science Foundation. Grant
Numbers: GAP501/11/1150, GA13-40637S, GP14-30004P'
author:
- first_name: Markéta
full_name: Pernisová, Markéta
last_name: Pernisová
- first_name: Tomas
full_name: Prat, Tomas
id: 3DA3BFEE-F248-11E8-B48F-1D18A9856A87
last_name: Prat
- first_name: Peter
full_name: Grones, Peter
id: 399876EC-F248-11E8-B48F-1D18A9856A87
last_name: Grones
- first_name: Danka
full_name: Haruštiaková, Danka
last_name: Haruštiaková
- first_name: Martina
full_name: Matonohova, Martina
last_name: Matonohova
- first_name: Lukáš
full_name: Spíchal, Lukáš
last_name: Spíchal
- first_name: Tomasz
full_name: Nodzyński, Tomasz
last_name: Nodzyński
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Jan
full_name: Hejátko, Jan
last_name: Hejátko
citation:
ama: Pernisová M, Prat T, Grones P, et al. Cytokinins influence root gravitropism
via differential regulation of auxin transporter expression and localization in
Arabidopsis. New Phytologist. 2016;212(2):497-509. doi:10.1111/nph.14049
apa: Pernisová, M., Prat, T., Grones, P., Haruštiaková, D., Matonohova, M., Spíchal,
L., … Hejátko, J. (2016). Cytokinins influence root gravitropism via differential
regulation of auxin transporter expression and localization in Arabidopsis. New
Phytologist. Wiley-Blackwell. https://doi.org/10.1111/nph.14049
chicago: Pernisová, Markéta, Tomas Prat, Peter Grones, Danka Haruštiaková, Martina
Matonohova, Lukáš Spíchal, Tomasz Nodzyński, Jiří Friml, and Jan Hejátko. “Cytokinins
Influence Root Gravitropism via Differential Regulation of Auxin Transporter Expression
and Localization in Arabidopsis.” New Phytologist. Wiley-Blackwell, 2016.
https://doi.org/10.1111/nph.14049.
ieee: M. Pernisová et al., “Cytokinins influence root gravitropism via differential
regulation of auxin transporter expression and localization in Arabidopsis,” New
Phytologist, vol. 212, no. 2. Wiley-Blackwell, pp. 497–509, 2016.
ista: Pernisová M, Prat T, Grones P, Haruštiaková D, Matonohova M, Spíchal L, Nodzyński
T, Friml J, Hejátko J. 2016. Cytokinins influence root gravitropism via differential
regulation of auxin transporter expression and localization in Arabidopsis. New
Phytologist. 212(2), 497–509.
mla: Pernisová, Markéta, et al. “Cytokinins Influence Root Gravitropism via Differential
Regulation of Auxin Transporter Expression and Localization in Arabidopsis.” New
Phytologist, vol. 212, no. 2, Wiley-Blackwell, 2016, pp. 497–509, doi:10.1111/nph.14049.
short: M. Pernisová, T. Prat, P. Grones, D. Haruštiaková, M. Matonohova, L. Spíchal,
T. Nodzyński, J. Friml, J. Hejátko, New Phytologist 212 (2016) 497–509.
date_created: 2018-12-11T11:51:38Z
date_published: 2016-10-01T00:00:00Z
date_updated: 2021-01-12T06:50:13Z
day: '01'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.1111/nph.14049
file:
- access_level: open_access
checksum: 27fd841ceaf0403559d7048ef51500f9
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:14:53Z
date_updated: 2020-07-14T12:44:47Z
file_id: '5108'
file_name: IST-2018-1006-v1+1_Pernisova_NewPhytol_2016_peer_review.pdf
file_size: 972763
relation: main_file
file_date_updated: 2020-07-14T12:44:47Z
has_accepted_license: '1'
intvolume: ' 212'
issue: '2'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Submitted Version
page: 497 - 509
publication: New Phytologist
publication_status: published
publisher: Wiley-Blackwell
publist_id: '5839'
pubrep_id: '1006'
quality_controlled: '1'
scopus_import: 1
status: public
title: Cytokinins influence root gravitropism via differential regulation of auxin
transporter expression and localization in Arabidopsis
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 212
year: '2016'
...
---
_id: '1410'
abstract:
- lang: eng
text: The pollen grains arise after meiosis of pollen mother cells within the anthers.
A series of complex structural changes follows, generating mature pollen grains
capable of performing the double fertilization of the female megasporophyte. Several
signaling molecules, including hormones and lipids, have been involved in the
regulation and appropriate control of pollen development. Phosphatidylinositol
4-phophate 5-kinases (PIP5K), which catalyze the biosynthesis of the phosphoinositide
PtdIns(4,5)P2, are important for tip polar growth of root hairs and pollen tubes,
embryo development, vegetative plant growth, and responses to the environment.
Here, we report a role of PIP5Ks during microgametogenesis. PIP5K1 and PIP5K2
are expressed during early stages of pollen development and their transcriptional
activity respond to auxin in pollen grains. Early male gametophytic lethality
to certain grade was observed in both pip5k1-/- and pip5k2-/- single mutants.
The number of pip5k mutant alleles is directly related to the frequency of aborted
pollen grains suggesting the two genes are involved in the same function. Indeed
PIP5K1 and PIP5K2 are functionally redundant since homozygous double mutants did
not render viable pollen grains. The loss of function of PIP5K1 and PIP5K2results
in defects in vacuole morphology in pollen at the later stages and epidermal root
cells. Our results show that PIP5K1, PIP5K2 and phosphoinositide signaling are
important cues for early developmental stages and vacuole formation during microgametogenesis.
acknowledgement: the Odysseus Program of the Research Foundation-Flanders [G091608]
to JF.
author:
- first_name: José
full_name: Ugalde, José
last_name: Ugalde
- first_name: Cecilia
full_name: Rodríguez Furlán, Cecilia
last_name: Rodríguez Furlán
- first_name: Riet
full_name: De Rycke, Riet
last_name: De Rycke
- first_name: Lorena
full_name: Norambuena, Lorena
last_name: Norambuena
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Gabriel
full_name: León, Gabriel
last_name: León
- first_name: Ricardo
full_name: Tejos, Ricardo
last_name: Tejos
citation:
ama: Ugalde J, Rodríguez Furlán C, De Rycke R, et al. Phosphatidylinositol 4-phosphate
5-kinases 1 and 2 are involved in the regulation of vacuole morphology during
Arabidopsis thaliana pollen development. Plant Science. 2016;250:10-19.
doi:10.1016/j.plantsci.2016.05.014
apa: Ugalde, J., Rodríguez Furlán, C., De Rycke, R., Norambuena, L., Friml, J.,
León, G., & Tejos, R. (2016). Phosphatidylinositol 4-phosphate 5-kinases 1
and 2 are involved in the regulation of vacuole morphology during Arabidopsis
thaliana pollen development. Plant Science. Elsevier. https://doi.org/10.1016/j.plantsci.2016.05.014
chicago: Ugalde, José, Cecilia Rodríguez Furlán, Riet De Rycke, Lorena Norambuena,
Jiří Friml, Gabriel León, and Ricardo Tejos. “Phosphatidylinositol 4-Phosphate
5-Kinases 1 and 2 Are Involved in the Regulation of Vacuole Morphology during
Arabidopsis Thaliana Pollen Development.” Plant Science. Elsevier, 2016.
https://doi.org/10.1016/j.plantsci.2016.05.014.
ieee: J. Ugalde et al., “Phosphatidylinositol 4-phosphate 5-kinases 1 and
2 are involved in the regulation of vacuole morphology during Arabidopsis thaliana
pollen development,” Plant Science, vol. 250. Elsevier, pp. 10–19, 2016.
ista: Ugalde J, Rodríguez Furlán C, De Rycke R, Norambuena L, Friml J, León G, Tejos
R. 2016. Phosphatidylinositol 4-phosphate 5-kinases 1 and 2 are involved in the
regulation of vacuole morphology during Arabidopsis thaliana pollen development.
Plant Science. 250, 10–19.
mla: Ugalde, José, et al. “Phosphatidylinositol 4-Phosphate 5-Kinases 1 and 2 Are
Involved in the Regulation of Vacuole Morphology during Arabidopsis Thaliana Pollen
Development.” Plant Science, vol. 250, Elsevier, 2016, pp. 10–19, doi:10.1016/j.plantsci.2016.05.014.
short: J. Ugalde, C. Rodríguez Furlán, R. De Rycke, L. Norambuena, J. Friml, G.
León, R. Tejos, Plant Science 250 (2016) 10–19.
date_created: 2018-12-11T11:51:51Z
date_published: 2016-09-01T00:00:00Z
date_updated: 2021-01-12T06:50:33Z
day: '01'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.1016/j.plantsci.2016.05.014
external_id:
pmid:
- '27457979'
file:
- access_level: open_access
checksum: ca08de036e6ddc81e6f760e0ccdebd3f
content_type: application/pdf
creator: dernst
date_created: 2019-04-17T07:41:57Z
date_updated: 2020-07-14T12:44:53Z
file_id: '6331'
file_name: 2016_PlantScience_Ugalde.pdf
file_size: 4338545
relation: main_file
file_date_updated: 2020-07-14T12:44:53Z
has_accepted_license: '1'
intvolume: ' 250'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Submitted Version
page: 10 - 19
pmid: 1
publication: Plant Science
publication_status: published
publisher: Elsevier
publist_id: '5797'
pubrep_id: '1005'
quality_controlled: '1'
scopus_import: 1
status: public
title: Phosphatidylinositol 4-phosphate 5-kinases 1 and 2 are involved in the regulation
of vacuole morphology during Arabidopsis thaliana pollen development
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 250
year: '2016'
...
---
_id: '1417'
abstract:
- lang: eng
text: Plant development mediated by the phytohormone auxin depends on tightly controlled
cellular auxin levels at its target tissue that are largely established by intercellular
and intracellular auxin transport mediated by PIN auxin transporters. Among the
eight members of the Arabidopsis PIN family, PIN6 is the least characterized candidate.
In this study we generated functional, fluorescent protein-tagged PIN6 proteins
and performed comprehensive analysis of their subcellular localization and also
performed a detailed functional characterization of PIN6 and its developmental
roles. The localization study of PIN6 revealed a dual localization at the plasma
membrane (PM) and endoplasmic reticulum (ER). Transport and metabolic profiling
assays in cultured cells and Arabidopsis strongly suggest that PIN6 mediates both
auxin transport across the PM and intracellular auxin homeostasis, including the
regulation of free auxin and auxin conjugates levels. As evidenced by the loss-
and gain-of-function analysis, the complex function of PIN6 in auxin transport
and homeostasis is required for auxin distribution during lateral and adventitious
root organogenesis and for progression of these developmental processes. These
results illustrate a unique position of PIN6 within the family of PIN auxin transporters
and further add complexity to the developmentally crucial process of auxin transport.
acknowledgement: This work was supported by the European Research Council (project
ERC-2011-StG-20101109-PSDP, project CEITEC (CZ.1.05/1.1.00/02.0068) and the Czech
Science Foundation GACR (project no. 13-4063 7S to J.F.)
author:
- first_name: Sibu
full_name: Simon, Sibu
id: 4542EF9A-F248-11E8-B48F-1D18A9856A87
last_name: Simon
orcid: 0000-0002-1998-6741
- first_name: Petr
full_name: Skůpa, Petr
last_name: Skůpa
- first_name: Tom
full_name: Viaene, Tom
last_name: Viaene
- first_name: Marta
full_name: Zwiewka, Marta
last_name: Zwiewka
- first_name: Ricardo
full_name: Tejos, Ricardo
last_name: Tejos
- first_name: Petr
full_name: Klíma, Petr
last_name: Klíma
- first_name: Mária
full_name: Čarná, Mária
last_name: Čarná
- first_name: Jakub
full_name: Rolčík, Jakub
last_name: Rolčík
- first_name: Riet
full_name: De Rycke, Riet
last_name: De Rycke
- first_name: Ignacio
full_name: Moreno, Ignacio
last_name: Moreno
- first_name: Petre
full_name: Dobrev, Petre
last_name: Dobrev
- first_name: Ariel
full_name: Orellana, Ariel
last_name: Orellana
- first_name: Eva
full_name: Zažímalová, Eva
last_name: Zažímalová
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Simon S, Skůpa P, Viaene T, et al. PIN6 auxin transporter at endoplasmic reticulum
and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis.
New Phytologist. 2016;211(1):65-74. doi:10.1111/nph.14019
apa: Simon, S., Skůpa, P., Viaene, T., Zwiewka, M., Tejos, R., Klíma, P., … Friml,
J. (2016). PIN6 auxin transporter at endoplasmic reticulum and plasma membrane
mediates auxin homeostasis and organogenesis in Arabidopsis. New Phytologist.
Wiley-Blackwell. https://doi.org/10.1111/nph.14019
chicago: Simon, Sibu, Petr Skůpa, Tom Viaene, Marta Zwiewka, Ricardo Tejos, Petr
Klíma, Mária Čarná, et al. “PIN6 Auxin Transporter at Endoplasmic Reticulum and
Plasma Membrane Mediates Auxin Homeostasis and Organogenesis in Arabidopsis.”
New Phytologist. Wiley-Blackwell, 2016. https://doi.org/10.1111/nph.14019.
ieee: S. Simon et al., “PIN6 auxin transporter at endoplasmic reticulum and
plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis,”
New Phytologist, vol. 211, no. 1. Wiley-Blackwell, pp. 65–74, 2016.
ista: Simon S, Skůpa P, Viaene T, Zwiewka M, Tejos R, Klíma P, Čarná M, Rolčík J,
De Rycke R, Moreno I, Dobrev P, Orellana A, Zažímalová E, Friml J. 2016. PIN6
auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin
homeostasis and organogenesis in Arabidopsis. New Phytologist. 211(1), 65–74.
mla: Simon, Sibu, et al. “PIN6 Auxin Transporter at Endoplasmic Reticulum and Plasma
Membrane Mediates Auxin Homeostasis and Organogenesis in Arabidopsis.” New
Phytologist, vol. 211, no. 1, Wiley-Blackwell, 2016, pp. 65–74, doi:10.1111/nph.14019.
short: S. Simon, P. Skůpa, T. Viaene, M. Zwiewka, R. Tejos, P. Klíma, M. Čarná,
J. Rolčík, R. De Rycke, I. Moreno, P. Dobrev, A. Orellana, E. Zažímalová, J. Friml,
New Phytologist 211 (2016) 65–74.
date_created: 2018-12-11T11:51:54Z
date_published: 2016-07-01T00:00:00Z
date_updated: 2021-01-12T06:50:36Z
day: '01'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.1111/nph.14019
file:
- access_level: open_access
checksum: 23522ced3508ffe7a4f247c4230e6493
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:13:32Z
date_updated: 2020-07-14T12:44:53Z
file_id: '5016'
file_name: IST-2018-1004-v1+1_Simon_NewPhytol_2016_proof.pdf
file_size: 3828383
relation: main_file
file_date_updated: 2020-07-14T12:44:53Z
has_accepted_license: '1'
intvolume: ' 211'
issue: '1'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Submitted Version
page: 65 - 74
publication: New Phytologist
publication_status: published
publisher: Wiley-Blackwell
publist_id: '5790'
pubrep_id: '1004'
quality_controlled: '1'
scopus_import: 1
status: public
title: PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates
auxin homeostasis and organogenesis in Arabidopsis
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 211
year: '2016'
...
---
_id: '1482'
abstract:
- lang: eng
text: Plants have the ability to continously generate new organs by maintaining
populations of stem cells throught their lives. The shoot apical meristem (SAM)
provides a stable environment for the maintenance of stem cells. All cells inside
the SAM divide, yet boundaries and patterns are maintained. Experimental evidence
indicates that patterning is independent of cell lineage, thus a dynamic self-regulatory
mechanism is required. A pivotal role in the organization of the SAM is played
by the WUSCHEL gene (WUS). An important question in this regard is that how WUS
expression is positioned in the SAM via a cell-lineage independent signaling mechanism.
In this study we demonstrate via mathematical modeling that a combination of an
inhibitor of the Cytokinin (CK) receptor, Arabidopsis histidine kinase 4 (AHK4)
and two morphogens originating from the top cell layer, can plausibly account
for the cell lineage-independent centering of WUS expression within SAM. Furthermore,
our laser ablation and microsurgical experiments support the hypothesis that patterning
in SAM occurs at the level of CK reception and signaling. The model suggests that
the interplay between CK signaling, WUS/CLV feedback loop and boundary signals
can account for positioning of the WUS expression, and provides directions for
further experimental investigation.
acknowledgement: We thank J. Traas, B. Müller and V. Reddy for providing seed materials
and Y. Deb for advice regarding the laser ablation experiments. We specially thank
Thomas Laux for stimulating discussions and support in the initial phase of this
project.
article_number: e0147830
author:
- first_name: Milad
full_name: Adibi, Milad
last_name: Adibi
- first_name: Saiko
full_name: Yoshida, Saiko
id: 2E46069C-F248-11E8-B48F-1D18A9856A87
last_name: Yoshida
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
- first_name: Christian
full_name: Fleck, Christian
last_name: Fleck
citation:
ama: Adibi M, Yoshida S, Weijers D, Fleck C. Centering the organizing center in
the Arabidopsis thaliana shoot apical meristem by a combination of cytokinin signaling
and self-organization. PLoS One. 2016;11(2). doi:10.1371/journal.pone.0147830
apa: Adibi, M., Yoshida, S., Weijers, D., & Fleck, C. (2016). Centering the
organizing center in the Arabidopsis thaliana shoot apical meristem by a combination
of cytokinin signaling and self-organization. PLoS One. Public Library
of Science. https://doi.org/10.1371/journal.pone.0147830
chicago: Adibi, Milad, Saiko Yoshida, Dolf Weijers, and Christian Fleck. “Centering
the Organizing Center in the Arabidopsis Thaliana Shoot Apical Meristem by a Combination
of Cytokinin Signaling and Self-Organization.” PLoS One. Public Library
of Science, 2016. https://doi.org/10.1371/journal.pone.0147830.
ieee: M. Adibi, S. Yoshida, D. Weijers, and C. Fleck, “Centering the organizing
center in the Arabidopsis thaliana shoot apical meristem by a combination of cytokinin
signaling and self-organization,” PLoS One, vol. 11, no. 2. Public Library
of Science, 2016.
ista: Adibi M, Yoshida S, Weijers D, Fleck C. 2016. Centering the organizing center
in the Arabidopsis thaliana shoot apical meristem by a combination of cytokinin
signaling and self-organization. PLoS One. 11(2), e0147830.
mla: Adibi, Milad, et al. “Centering the Organizing Center in the Arabidopsis Thaliana
Shoot Apical Meristem by a Combination of Cytokinin Signaling and Self-Organization.”
PLoS One, vol. 11, no. 2, e0147830, Public Library of Science, 2016, doi:10.1371/journal.pone.0147830.
short: M. Adibi, S. Yoshida, D. Weijers, C. Fleck, PLoS One 11 (2016).
date_created: 2018-12-11T11:52:17Z
date_published: 2016-02-01T00:00:00Z
date_updated: 2021-01-12T06:51:03Z
day: '01'
ddc:
- '570'
department:
- _id: JiFr
doi: 10.1371/journal.pone.0147830
file:
- access_level: open_access
checksum: 6066146e527335030f83aa5924ab72a6
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:14:16Z
date_updated: 2020-07-14T12:44:57Z
file_id: '5066'
file_name: IST-2016-521-v1+1_journal.pone.0147830.PDF
file_size: 4297148
relation: main_file
file_date_updated: 2020-07-14T12:44:57Z
has_accepted_license: '1'
intvolume: ' 11'
issue: '2'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
publication: PLoS One
publication_status: published
publisher: Public Library of Science
publist_id: '5711'
pubrep_id: '521'
quality_controlled: '1'
scopus_import: 1
status: public
title: Centering the organizing center in the Arabidopsis thaliana shoot apical meristem
by a combination of cytokinin signaling and self-organization
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 11
year: '2016'
...
---
_id: '1484'
acknowledgement: We thank Maciek Adamowski for helpful discussions and Qiang Zhu and
Israel Ausin for critical reading of the manuscript. We sincerely apologize to colleagues
whose work we could not include owing to space limitations.
article_type: review
author:
- first_name: Xu
full_name: Chen, Xu
id: 4E5ADCAA-F248-11E8-B48F-1D18A9856A87
last_name: Chen
- first_name: Shuang
full_name: Wu, Shuang
last_name: Wu
- first_name: Zengyu
full_name: Liu, Zengyu
last_name: Liu
- first_name: Jiřĺ
full_name: Friml, Jiřĺ
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Chen X, Wu S, Liu Z, Friml J. Environmental and endogenous control of cortical
microtubule orientation. Trends in Cell Biology. 2016;26(6):409-419. doi:10.1016/j.tcb.2016.02.003
apa: Chen, X., Wu, S., Liu, Z., & Friml, J. (2016). Environmental and endogenous
control of cortical microtubule orientation. Trends in Cell Biology. Cell
Press. https://doi.org/10.1016/j.tcb.2016.02.003
chicago: Chen, Xu, Shuang Wu, Zengyu Liu, and Jiří Friml. “Environmental and Endogenous
Control of Cortical Microtubule Orientation.” Trends in Cell Biology. Cell
Press, 2016. https://doi.org/10.1016/j.tcb.2016.02.003.
ieee: X. Chen, S. Wu, Z. Liu, and J. Friml, “Environmental and endogenous control
of cortical microtubule orientation,” Trends in Cell Biology, vol. 26,
no. 6. Cell Press, pp. 409–419, 2016.
ista: Chen X, Wu S, Liu Z, Friml J. 2016. Environmental and endogenous control of
cortical microtubule orientation. Trends in Cell Biology. 26(6), 409–419.
mla: Chen, Xu, et al. “Environmental and Endogenous Control of Cortical Microtubule
Orientation.” Trends in Cell Biology, vol. 26, no. 6, Cell Press, 2016,
pp. 409–19, doi:10.1016/j.tcb.2016.02.003.
short: X. Chen, S. Wu, Z. Liu, J. Friml, Trends in Cell Biology 26 (2016) 409–419.
date_created: 2018-12-11T11:52:17Z
date_published: 2016-06-01T00:00:00Z
date_updated: 2021-01-12T06:51:04Z
day: '01'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.1016/j.tcb.2016.02.003
file:
- access_level: open_access
checksum: b229e5bb4676ec3e27b7b9ea603b3a63
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:15:34Z
date_updated: 2020-07-14T12:44:57Z
file_id: '5155'
file_name: IST-2018-1002-v1+1_Chen_TICB_2016_proofs.pdf
file_size: 2329117
relation: main_file
file_date_updated: 2020-07-14T12:44:57Z
has_accepted_license: '1'
intvolume: ' 26'
issue: '6'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Submitted Version
page: 409 - 419
publication: Trends in Cell Biology
publication_status: published
publisher: Cell Press
publist_id: '5704'
pubrep_id: '1002'
quality_controlled: '1'
scopus_import: 1
status: public
title: Environmental and endogenous control of cortical microtubule orientation
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 26
year: '2016'
...
---
_id: '1641'
abstract:
- lang: eng
text: The plant hormone auxin (indole-3-acetic acid) is a major regulator of plant
growth and development including embryo and root patterning, lateral organ formation
and growth responses to environmental stimuli. Auxin is directionally transported
from cell to cell by the action of specific auxin influx [AUXIN-RESISTANT1 (AUX1)]
and efflux [PIN-FORMED (PIN)] transport regulators, whose polar, subcellular localizations
are aligned with the direction of the auxin flow. Auxin itself regulates its own
transport by modulation of the expression and subcellular localization of the
auxin transporters. Increased auxin levels promote the transcription of PIN2 and
AUX1 genes as well as stabilize PIN proteins at the plasma membrane, whereas prolonged
auxin exposure increases the turnover of PIN proteins and their degradation in
the vacuole. In this study, we applied a forward genetic approach, to identify
molecular components playing a role in the auxin-mediated degradation. We generated
EMS-mutagenized Arabidopsis PIN2::PIN2:GFP, AUX1::AUX1:YFP eir1aux1 populations
and designed a screen for mutants with persistently strong fluorescent signals
of the tagged PIN2 and AUX1 after prolonged treatment with the synthetic auxin
2,4-dichlorophenoxyacetic acid (2,4-D). This approach yielded novel auxin degradation
mutants defective in trafficking and degradation of PIN2 and AUX1 proteins and
established a role for auxin-mediated degradation in plant development.
acknowledgement: 'European Social Fund (CZ.1.07/2.3.00/20.0043) and the Czech Science
Foundation GAČR (GA13-40637S) to JF. '
author:
- first_name: Radka
full_name: Zemová, Radka
last_name: Zemová
- first_name: Marta
full_name: Zwiewka, Marta
last_name: Zwiewka
- first_name: Agnieszka
full_name: Bielach, Agnieszka
last_name: Bielach
- first_name: Hélène
full_name: Robert, Hélène
last_name: Robert
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Zemová R, Zwiewka M, Bielach A, Robert H, Friml J. A forward genetic screen
for new regulators of auxin mediated degradation of auxin transport proteins in
Arabidopsis thaliana. Journal of Plant Growth Regulation. 2016;35(2):465-476.
doi:10.1007/s00344-015-9553-2
apa: Zemová, R., Zwiewka, M., Bielach, A., Robert, H., & Friml, J. (2016). A
forward genetic screen for new regulators of auxin mediated degradation of auxin
transport proteins in Arabidopsis thaliana. Journal of Plant Growth Regulation.
Springer. https://doi.org/10.1007/s00344-015-9553-2
chicago: Zemová, Radka, Marta Zwiewka, Agnieszka Bielach, Hélène Robert, and Jiří
Friml. “A Forward Genetic Screen for New Regulators of Auxin Mediated Degradation
of Auxin Transport Proteins in Arabidopsis Thaliana.” Journal of Plant Growth
Regulation. Springer, 2016. https://doi.org/10.1007/s00344-015-9553-2.
ieee: R. Zemová, M. Zwiewka, A. Bielach, H. Robert, and J. Friml, “A forward genetic
screen for new regulators of auxin mediated degradation of auxin transport proteins
in Arabidopsis thaliana,” Journal of Plant Growth Regulation, vol. 35,
no. 2. Springer, pp. 465–476, 2016.
ista: Zemová R, Zwiewka M, Bielach A, Robert H, Friml J. 2016. A forward genetic
screen for new regulators of auxin mediated degradation of auxin transport proteins
in Arabidopsis thaliana. Journal of Plant Growth Regulation. 35(2), 465–476.
mla: Zemová, Radka, et al. “A Forward Genetic Screen for New Regulators of Auxin
Mediated Degradation of Auxin Transport Proteins in Arabidopsis Thaliana.” Journal
of Plant Growth Regulation, vol. 35, no. 2, Springer, 2016, pp. 465–76, doi:10.1007/s00344-015-9553-2.
short: R. Zemová, M. Zwiewka, A. Bielach, H. Robert, J. Friml, Journal of Plant
Growth Regulation 35 (2016) 465–476.
date_created: 2018-12-11T11:53:12Z
date_published: 2016-06-01T00:00:00Z
date_updated: 2021-01-12T06:52:11Z
day: '01'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.1007/s00344-015-9553-2
file:
- access_level: open_access
checksum: 0dc6a300cde6536ceedd2bcdd2060efb
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:08:34Z
date_updated: 2020-07-14T12:45:08Z
file_id: '4695'
file_name: IST-2018-1001-v1+1_Zemova_JPlantGrowthRegul_2016_proofs.pdf
file_size: 5637591
relation: main_file
file_date_updated: 2020-07-14T12:45:08Z
has_accepted_license: '1'
intvolume: ' 35'
issue: '2'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Preprint
page: 465 - 476
publication: Journal of Plant Growth Regulation
publication_status: published
publisher: Springer
publist_id: '5512'
pubrep_id: '1001'
quality_controlled: '1'
scopus_import: 1
status: public
title: A forward genetic screen for new regulators of auxin mediated degradation of
auxin transport proteins in Arabidopsis thaliana
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 35
year: '2016'
...
---
_id: '1346'
abstract:
- lang: eng
text: ATP production requires the establishment of an electrochemical proton gradient
across the inner mitochondrial membrane. Mitochondrial uncouplers dissipate this
proton gradient and disrupt numerous cellular processes, including vesicular trafficking,
mainly through energy depletion. Here we show that Endosidin9 (ES9), a novel mitochondrial
uncoupler, is a potent inhibitor of clathrin-mediated endocytosis (CME) in different
systems and that ES9 induces inhibition of CME not because of its effect on cellular
ATP, but rather due to its protonophore activity that leads to cytoplasm acidification.
We show that the known tyrosine kinase inhibitor tyrphostinA23, which is routinely
used to block CME, displays similar properties, thus questioning its use as a
specific inhibitor of cargo recognition by the AP-2 adaptor complex via tyrosine
motif-based endocytosis signals. Furthermore, we show that cytoplasm acidification
dramatically affects the dynamics and recruitment of clathrin and associated adaptors,
and leads to reduction of phosphatidylinositol 4,5-biphosphate from the plasma
membrane.
acknowledgement: "We thank Yvon Jaillais, Ikuko Hara-Nishimura, Akihiko Nakano, Takashi
Ueda and Jinxing Lin for providing materials, Natasha Raikhel, Glenn Hicks, Steffen
Vanneste, and Ricardo Tejos for useful suggestions, Patrick Callaerts for providing
S2 Drosophila cell cultures, Michael Sixt for providing HeLa cells, Annick Bleys
for literature searches, VIB Bio Imaging Core for help with imaging conditions and
Martine De Cock for help in preparing the article. This work was supported by the
Agency for Innovation by Science\r\nand Technology for a pre-doctoral fellowship
to W.D.; the Research fund KU Leuven\r\n(GOA), a Methusalem grant of the Flemish
government and VIB to S.K., J.K. and P.V.;\r\nby the Netherlands Organisation for
Scientific Research (NWO) for ALW grants\r\n846.11.002 (C.T.) and 867.15.020 (T.M.);
the European Research Council (project\r\nERC-2011-StG-20101109 PSDP) (to J.F.);
a European Research Council (ERC) Starting\r\nGrant (grant 260678) (to P.V.), the
Research Foundation-Flanders (grants G.0747.09,\r\nG094011 and G095511) (to P.V.),
the Hercules Foundation, an Interuniversity Attraction\r\nPoles Poles Program, initiated
by the Belgian State, Science Policy Office (to P.V.),\r\nthe Swedish VetenskapsRådet
grant to O.K., the Ghent University ‘Bijzonder\r\nOnderzoek Fonds’ (BOF) for a predoctoral
fellowship to F.A.O.-M., the Research\r\nFoundation-Flanders (FWO) to K.M. and E.R."
article_number: '11710'
author:
- first_name: Wim
full_name: Dejonghe, Wim
last_name: Dejonghe
- first_name: Sabine
full_name: Kuenen, Sabine
last_name: Kuenen
- first_name: Evelien
full_name: Mylle, Evelien
last_name: Mylle
- first_name: Mina K
full_name: Vasileva, Mina K
id: 3407EB18-F248-11E8-B48F-1D18A9856A87
last_name: Vasileva
- first_name: Olivier
full_name: Keech, Olivier
last_name: Keech
- first_name: Corrado
full_name: Viotti, Corrado
last_name: Viotti
- first_name: Jef
full_name: Swerts, Jef
last_name: Swerts
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: Fausto
full_name: Ortiz Morea, Fausto
last_name: Ortiz Morea
- first_name: Kiril
full_name: Mishev, Kiril
last_name: Mishev
- first_name: Simon
full_name: Delang, Simon
last_name: Delang
- first_name: Stefan
full_name: Scholl, Stefan
last_name: Scholl
- first_name: Xavier
full_name: Zarza, Xavier
last_name: Zarza
- first_name: Mareike
full_name: Heilmann, Mareike
last_name: Heilmann
- first_name: Jiorgos
full_name: Kourelis, Jiorgos
last_name: Kourelis
- first_name: Jaroslaw
full_name: Kasprowicz, Jaroslaw
last_name: Kasprowicz
- first_name: Le
full_name: Nguyen, Le
last_name: Nguyen
- first_name: Andrzej
full_name: Drozdzecki, Andrzej
last_name: Drozdzecki
- first_name: Isabelle
full_name: Van Houtte, Isabelle
last_name: Van Houtte
- first_name: Anna
full_name: Szatmári, Anna
last_name: Szatmári
- first_name: Mateusz
full_name: Majda, Mateusz
last_name: Majda
- first_name: Gary
full_name: Baisa, Gary
last_name: Baisa
- first_name: Sebastian
full_name: Bednarek, Sebastian
last_name: Bednarek
- first_name: Stéphanie
full_name: Robert, Stéphanie
last_name: Robert
- first_name: Dominique
full_name: Audenaert, Dominique
last_name: Audenaert
- first_name: Christa
full_name: Testerink, Christa
last_name: Testerink
- first_name: Teun
full_name: Munnik, Teun
last_name: Munnik
- first_name: Daniël
full_name: Van Damme, Daniël
last_name: Van Damme
- first_name: Ingo
full_name: Heilmann, Ingo
last_name: Heilmann
- first_name: Karin
full_name: Schumacher, Karin
last_name: Schumacher
- first_name: Johan
full_name: Winne, Johan
last_name: Winne
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Patrik
full_name: Verstreken, Patrik
last_name: Verstreken
- first_name: Eugenia
full_name: Russinova, Eugenia
last_name: Russinova
citation:
ama: Dejonghe W, Kuenen S, Mylle E, et al. Mitochondrial uncouplers inhibit clathrin-mediated
endocytosis largely through cytoplasmic acidification. Nature Communications.
2016;7. doi:10.1038/ncomms11710
apa: Dejonghe, W., Kuenen, S., Mylle, E., Vasileva, M. K., Keech, O., Viotti, C.,
… Russinova, E. (2016). Mitochondrial uncouplers inhibit clathrin-mediated endocytosis
largely through cytoplasmic acidification. Nature Communications. Nature
Publishing Group. https://doi.org/10.1038/ncomms11710
chicago: Dejonghe, Wim, Sabine Kuenen, Evelien Mylle, Mina K Vasileva, Olivier Keech,
Corrado Viotti, Jef Swerts, et al. “Mitochondrial Uncouplers Inhibit Clathrin-Mediated
Endocytosis Largely through Cytoplasmic Acidification.” Nature Communications.
Nature Publishing Group, 2016. https://doi.org/10.1038/ncomms11710.
ieee: W. Dejonghe et al., “Mitochondrial uncouplers inhibit clathrin-mediated
endocytosis largely through cytoplasmic acidification,” Nature Communications,
vol. 7. Nature Publishing Group, 2016.
ista: Dejonghe W, Kuenen S, Mylle E, Vasileva MK, Keech O, Viotti C, Swerts J, Fendrych
M, Ortiz Morea F, Mishev K, Delang S, Scholl S, Zarza X, Heilmann M, Kourelis
J, Kasprowicz J, Nguyen L, Drozdzecki A, Van Houtte I, Szatmári A, Majda M, Baisa
G, Bednarek S, Robert S, Audenaert D, Testerink C, Munnik T, Van Damme D, Heilmann
I, Schumacher K, Winne J, Friml J, Verstreken P, Russinova E. 2016. Mitochondrial
uncouplers inhibit clathrin-mediated endocytosis largely through cytoplasmic acidification.
Nature Communications. 7, 11710.
mla: Dejonghe, Wim, et al. “Mitochondrial Uncouplers Inhibit Clathrin-Mediated Endocytosis
Largely through Cytoplasmic Acidification.” Nature Communications, vol.
7, 11710, Nature Publishing Group, 2016, doi:10.1038/ncomms11710.
short: W. Dejonghe, S. Kuenen, E. Mylle, M.K. Vasileva, O. Keech, C. Viotti, J.
Swerts, M. Fendrych, F. Ortiz Morea, K. Mishev, S. Delang, S. Scholl, X. Zarza,
M. Heilmann, J. Kourelis, J. Kasprowicz, L. Nguyen, A. Drozdzecki, I. Van Houtte,
A. Szatmári, M. Majda, G. Baisa, S. Bednarek, S. Robert, D. Audenaert, C. Testerink,
T. Munnik, D. Van Damme, I. Heilmann, K. Schumacher, J. Winne, J. Friml, P. Verstreken,
E. Russinova, Nature Communications 7 (2016).
date_created: 2018-12-11T11:51:30Z
date_published: 2016-06-08T00:00:00Z
date_updated: 2023-09-07T12:54:35Z
day: '08'
ddc:
- '570'
department:
- _id: JiFr
doi: 10.1038/ncomms11710
ec_funded: 1
file:
- access_level: open_access
checksum: e8dc81b3e44db5a7718d7f1501ce1aa7
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:18:47Z
date_updated: 2020-07-14T12:44:45Z
file_id: '5369'
file_name: IST-2016-653-v1+1_ncomms11710_1_.pdf
file_size: 3532505
relation: main_file
file_date_updated: 2020-07-14T12:44:45Z
has_accepted_license: '1'
intvolume: ' 7'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Nature Communications
publication_status: published
publisher: Nature Publishing Group
publist_id: '5906'
pubrep_id: '653'
quality_controlled: '1'
related_material:
record:
- id: '7172'
relation: dissertation_contains
status: public
scopus_import: 1
status: public
title: Mitochondrial uncouplers inhibit clathrin-mediated endocytosis largely through
cytoplasmic acidification
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 7
year: '2016'
...
---
_id: '510'
abstract:
- lang: eng
text: 'The CLE (CLAVATA3/Embryo Surrounding Region-related) peptides are small secreted
signaling peptides that are primarily involved in the regulation of stem cell
homeostasis in different plant meristems. Particularly, the characterization of
the CLE41-PXY/TDR signaling pathway has greatly advanced our understanding on
the potential roles of CLE peptides in vascular development and wood formation.
Nevertheless, our knowledge on this gene family in a tree species is limited.
In a recent study, we reported on a systematically investigation of the CLE gene
family in Populus trichocarpa . The potential roles of PtCLE genes were studied
by comparative analysis and transcriptional pro fi ling. Among fi fty PtCLE members,
many PtCLE proteins share identical CLE motifs or contain the same CLE motif as
that of AtCLEs, while PtCLE genes exhibited either comparable or distinct expression
patterns comparing to their Arabidopsis counterparts. These fi ndings indicate
the existence of both functional conservation and functional divergence between
PtCLEs and their AtCLE orthologues. Our results provide valuable resources for
future functional investigations of these critical signaling molecules in woody
plants. '
acknowledgement: 'We are grateful to Dr. Long (Laboratoire de Reproduction et Developpement
des Plantes,CNRS,INRA,ENSLyon,UCBL,Universite de Lyon,France)for critical reading
of the article. Work in our group is supported by the National Natural Science Foundation
of China (31271575; 31200902), the Fundamental Research Funds for the Central Univ
ersities (GK201103005), the Specialized Research Fund for the Doctoral Program of
Higher Education from the Ministry of Education of China (20120202120009), the Scientific
Research Foundation for the Returned Overseas Chinese Scholars, State Education
Ministry, and the Natural Science Basic Research Plan in Shaanxi Province of China
(2014JM3064). '
article_number: e1191734
article_processing_charge: No
author:
- first_name: Zhijun
full_name: Liu, Zhijun
last_name: Liu
- first_name: 'Nan'
full_name: Yang, Nan
last_name: Yang
- first_name: Yanting
full_name: Lv, Yanting
last_name: Lv
- first_name: Lixia
full_name: Pan, Lixia
last_name: Pan
- first_name: Shuo
full_name: Lv, Shuo
last_name: Lv
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
- first_name: Guodong
full_name: Wang, Guodong
last_name: Wang
citation:
ama: Liu Z, Yang N, Lv Y, et al. The CLE gene family in Populus trichocarpa. Plant
Signaling & Behavior. 2016;11(6). doi:10.1080/15592324.2016.1191734
apa: Liu, Z., Yang, N., Lv, Y., Pan, L., Lv, S., Han, H., & Wang, G. (2016).
The CLE gene family in Populus trichocarpa. Plant Signaling & Behavior.
Taylor & Francis. https://doi.org/10.1080/15592324.2016.1191734
chicago: Liu, Zhijun, Nan Yang, Yanting Lv, Lixia Pan, Shuo Lv, Huibin Han, and
Guodong Wang. “The CLE Gene Family in Populus Trichocarpa.” Plant Signaling
& Behavior. Taylor & Francis, 2016. https://doi.org/10.1080/15592324.2016.1191734.
ieee: Z. Liu et al., “The CLE gene family in Populus trichocarpa,” Plant
Signaling & Behavior, vol. 11, no. 6. Taylor & Francis, 2016.
ista: Liu Z, Yang N, Lv Y, Pan L, Lv S, Han H, Wang G. 2016. The CLE gene family
in Populus trichocarpa. Plant Signaling & Behavior. 11(6), e1191734.
mla: Liu, Zhijun, et al. “The CLE Gene Family in Populus Trichocarpa.” Plant
Signaling & Behavior, vol. 11, no. 6, e1191734, Taylor & Francis,
2016, doi:10.1080/15592324.2016.1191734.
short: Z. Liu, N. Yang, Y. Lv, L. Pan, S. Lv, H. Han, G. Wang, Plant Signaling &
Behavior 11 (2016).
date_created: 2018-12-11T11:46:53Z
date_published: 2016-06-02T00:00:00Z
date_updated: 2023-10-17T11:13:40Z
day: '02'
department:
- _id: JiFr
doi: 10.1080/15592324.2016.1191734
intvolume: ' 11'
issue: '6'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973754/
month: '06'
oa: 1
oa_version: Submitted Version
publication: Plant Signaling & Behavior
publication_status: published
publisher: Taylor & Francis
publist_id: '7308'
quality_controlled: '1'
scopus_import: '1'
status: public
title: The CLE gene family in Populus trichocarpa
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 11
year: '2016'
...
---
_id: '1274'
abstract:
- lang: eng
text: Synchronized tissue polarization during regeneration or de novo vascular tissue
formation is a plant-specific example of intercellular communication and coordinated
development. According to the canalization hypothesis, the plant hormone auxin
serves as polarizing signal that mediates directional channel formation underlying
the spatio-temporal vasculature patterning. A necessary part of canalization is
a positive feedback between auxin signaling and polarity of the intercellular
auxin flow. The cellular and molecular mechanisms of this process are still poorly
understood, not the least, because of a lack of a suitable model system. We show
that the main genetic model plant, Arabidopsis (Arabidopsis thaliana) can be used
to study the canalization during vascular cambium regeneration and new vasculature
formation. We monitored localized auxin responses, directional auxin-transport
channels formation, and establishment of new vascular cambium polarity during
regenerative processes after stem wounding. The increased auxin response above
and around the wound preceded the formation of PIN1 auxin transporter-marked channels
from the primarily homogenous tissue and the transient, gradual changes in PIN1
localization preceded the polarity of newly formed vascular tissue. Thus, Arabidopsis
is a useful model for studies of coordinated tissue polarization and vasculature
formation after wounding allowing for genetic and mechanistic dissection of the
canalization hypothesis.
acknowledgement: We wish to thank Prof. Ewa U. Kurczyńska for initiation of this work
and valuable advices. We thank Martine De Cock for help in preparing the manuscript.
This work was supported by the European Research Council (project ERC-2011-StG-20101109-PSDP),
the European Social Fund (CZ.1.07/2.3.00/20.0043), and the Czech Science Foundation
GAČR (GA13-40637 S) to J.F., (GA 13-39982S) to E.B. and E.M. and in part by the
European Regional Development Fund (project “CEITEC, Central European Institute
of Technology”, CZ.1.05/1.1.00/02.0068).
article_number: '33754'
article_processing_charge: No
author:
- first_name: Ewa
full_name: Mazur, Ewa
last_name: Mazur
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Mazur E, Benková E, Friml J. Vascular cambium regeneration and vessel formation
in wounded inflorescence stems of Arabidopsis. Scientific Reports. 2016;6.
doi:10.1038/srep33754
apa: Mazur, E., Benková, E., & Friml, J. (2016). Vascular cambium regeneration
and vessel formation in wounded inflorescence stems of Arabidopsis. Scientific
Reports. Nature Publishing Group. https://doi.org/10.1038/srep33754
chicago: Mazur, Ewa, Eva Benková, and Jiří Friml. “Vascular Cambium Regeneration
and Vessel Formation in Wounded Inflorescence Stems of Arabidopsis.” Scientific
Reports. Nature Publishing Group, 2016. https://doi.org/10.1038/srep33754.
ieee: E. Mazur, E. Benková, and J. Friml, “Vascular cambium regeneration and vessel
formation in wounded inflorescence stems of Arabidopsis,” Scientific Reports,
vol. 6. Nature Publishing Group, 2016.
ista: Mazur E, Benková E, Friml J. 2016. Vascular cambium regeneration and vessel
formation in wounded inflorescence stems of Arabidopsis. Scientific Reports. 6,
33754.
mla: Mazur, Ewa, et al. “Vascular Cambium Regeneration and Vessel Formation in Wounded
Inflorescence Stems of Arabidopsis.” Scientific Reports, vol. 6, 33754,
Nature Publishing Group, 2016, doi:10.1038/srep33754.
short: E. Mazur, E. Benková, J. Friml, Scientific Reports 6 (2016).
date_created: 2018-12-11T11:51:05Z
date_published: 2016-09-21T00:00:00Z
date_updated: 2024-02-12T12:03:42Z
day: '21'
ddc:
- '581'
department:
- _id: EvBe
- _id: JiFr
doi: 10.1038/srep33754
external_id:
pmid:
- '27649687'
file:
- access_level: open_access
checksum: ee371fbc9124ad93157a95829264e4fe
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:13:25Z
date_updated: 2020-07-14T12:44:42Z
file_id: '5008'
file_name: IST-2016-692-v1+1_srep33754.pdf
file_size: 2895147
relation: main_file
file_date_updated: 2020-07-14T12:44:42Z
has_accepted_license: '1'
intvolume: ' 6'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
publication: Scientific Reports
publication_status: published
publisher: Nature Publishing Group
publist_id: '6042'
pubrep_id: '692'
quality_controlled: '1'
related_material:
record:
- id: '545'
relation: later_version
status: public
scopus_import: '1'
status: public
title: Vascular cambium regeneration and vessel formation in wounded inflorescence
stems of Arabidopsis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 6
year: '2016'
...
---
_id: '1383'
abstract:
- lang: eng
text: In plants, vacuolar H+-ATPase (V-ATPase) activity acidifies both the trans-Golgi
network/early endosome (TGN/EE) and the vacuole. This dual V-ATPase function has
impeded our understanding of how the pH homeostasis within the plant TGN/EE controls
exo- and endocytosis. Here, we show that the weak V-ATPase mutant deetiolated3
(det3) displayed a pH increase in the TGN/EE, but not in the vacuole, strongly
impairing secretion and recycling of the brassinosteroid receptor and the cellulose
synthase complexes to the plasma membrane, in contrast to mutants lacking tonoplast-localized
V-ATPase activity only. The brassinosteroid insensitivity and the cellulose deficiency
defects in det3 were tightly correlated with reduced Golgi and TGN/EE motility.
Thus, our results provide strong evidence that acidification of the TGN/EE, but
not of the vacuole, is indispensable for functional secretion and recycling in
plants.
article_number: '15094'
article_processing_charge: No
article_type: original
author:
- first_name: Luo
full_name: Yu, Luo
last_name: Yu
- first_name: Stefan
full_name: Scholl, Stefan
last_name: Scholl
- first_name: Anett
full_name: Doering, Anett
last_name: Doering
- first_name: Zhang
full_name: Yi, Zhang
last_name: Yi
- first_name: Niloufer
full_name: Irani, Niloufer
last_name: Irani
- first_name: Simone
full_name: Di Rubbo, Simone
last_name: Di Rubbo
- first_name: Lutz
full_name: Neumetzler, Lutz
last_name: Neumetzler
- first_name: Praveen
full_name: Krishnamoorthy, Praveen
last_name: Krishnamoorthy
- first_name: Isabelle
full_name: Van Houtte, Isabelle
last_name: Van Houtte
- first_name: Evelien
full_name: Mylle, Evelien
last_name: Mylle
- first_name: Volker
full_name: Bischoff, Volker
last_name: Bischoff
- first_name: Samantha
full_name: Vernhettes, Samantha
last_name: Vernhettes
- first_name: Johan
full_name: Winne, Johan
last_name: Winne
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: York
full_name: Stierhof, York
last_name: Stierhof
- first_name: Karin
full_name: Schumacher, Karin
last_name: Schumacher
- first_name: Staffan
full_name: Persson, Staffan
last_name: Persson
- first_name: Eugenia
full_name: Russinova, Eugenia
last_name: Russinova
citation:
ama: Yu L, Scholl S, Doering A, et al. V-ATPase activity in the TGN/EE is required
for exocytosis and recycling in Arabidopsis. Nature Plants. 2015;1(7).
doi:10.1038/nplants.2015.94
apa: Yu, L., Scholl, S., Doering, A., Yi, Z., Irani, N., Di Rubbo, S., … Russinova,
E. (2015). V-ATPase activity in the TGN/EE is required for exocytosis and recycling
in Arabidopsis. Nature Plants. Nature Publishing Group. https://doi.org/10.1038/nplants.2015.94
chicago: Yu, Luo, Stefan Scholl, Anett Doering, Zhang Yi, Niloufer Irani, Simone
Di Rubbo, Lutz Neumetzler, et al. “V-ATPase Activity in the TGN/EE Is Required
for Exocytosis and Recycling in Arabidopsis.” Nature Plants. Nature Publishing
Group, 2015. https://doi.org/10.1038/nplants.2015.94.
ieee: L. Yu et al., “V-ATPase activity in the TGN/EE is required for exocytosis
and recycling in Arabidopsis,” Nature Plants, vol. 1, no. 7. Nature Publishing
Group, 2015.
ista: Yu L, Scholl S, Doering A, Yi Z, Irani N, Di Rubbo S, Neumetzler L, Krishnamoorthy
P, Van Houtte I, Mylle E, Bischoff V, Vernhettes S, Winne J, Friml J, Stierhof
Y, Schumacher K, Persson S, Russinova E. 2015. V-ATPase activity in the TGN/EE
is required for exocytosis and recycling in Arabidopsis. Nature Plants. 1(7),
15094.
mla: Yu, Luo, et al. “V-ATPase Activity in the TGN/EE Is Required for Exocytosis
and Recycling in Arabidopsis.” Nature Plants, vol. 1, no. 7, 15094, Nature
Publishing Group, 2015, doi:10.1038/nplants.2015.94.
short: L. Yu, S. Scholl, A. Doering, Z. Yi, N. Irani, S. Di Rubbo, L. Neumetzler,
P. Krishnamoorthy, I. Van Houtte, E. Mylle, V. Bischoff, S. Vernhettes, J. Winne,
J. Friml, Y. Stierhof, K. Schumacher, S. Persson, E. Russinova, Nature Plants
1 (2015).
date_created: 2018-12-11T11:51:42Z
date_published: 2015-07-06T00:00:00Z
date_updated: 2021-01-12T06:50:18Z
day: '06'
department:
- _id: JiFr
doi: 10.1038/nplants.2015.94
external_id:
pmid:
- '27250258'
intvolume: ' 1'
issue: '7'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4905525/
month: '07'
oa: 1
oa_version: Submitted Version
pmid: 1
publication: Nature Plants
publication_status: published
publisher: Nature Publishing Group
publist_id: '5827'
quality_controlled: '1'
scopus_import: 1
status: public
title: V-ATPase activity in the TGN/EE is required for exocytosis and recycling in
Arabidopsis
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 1
year: '2015'
...
---
_id: '1532'
abstract:
- lang: eng
text: Ammonium is the major nitrogen source in some plant ecosystems but is toxic
at high concentrations, especially when available as the exclusive nitrogen source.
Ammonium stress rapidly leads to various metabolic and hormonal imbalances that
ultimately inhibit root and shoot growth in many plant species, including Arabidopsis
thaliana (L.) Heynh. To identify molecular and genetic factors involved in seedling
survival with prolonged exclusive NH4+ nutrition, a transcriptomic analysis with
microarrays was used. Substantial transcriptional differences were most pronounced
in (NH4)2SO4-grown seedlings, compared with plants grown on KNO3 or NH4NO3. Consistent
with previous physiological analyses, major differences in the expression modules
of photosynthesis-related genes, an altered mitochondrial metabolism, differential
expression of the primary NH4+ assimilation, alteration of transporter gene expression
and crucial changes in cell wall biosynthesis were found. A major difference in
plant hormone responses, particularly of auxin but not cytokinin, was striking.
The activity of the DR5::GUS reporter revealed a dramatically decreased auxin
response in (NH4)2SO4-grown primary roots. The impaired root growth on (NH4)2SO4
was partially rescued by exogenous auxin or in specific mutants in the auxin pathway.
The data suggest that NH4+-induced nutritional and metabolic imbalances can be
partially overcome by elevated auxin levels.
article_processing_charge: No
article_type: original
author:
- first_name: Huaiyu
full_name: Yang, Huaiyu
last_name: Yang
- first_name: Jenny
full_name: Von Der Fecht Bartenbach, Jenny
last_name: Von Der Fecht Bartenbach
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Jan
full_name: Lohmann, Jan
last_name: Lohmann
- first_name: Benjamin
full_name: Neuhäuser, Benjamin
last_name: Neuhäuser
- first_name: Uwe
full_name: Ludewig, Uwe
last_name: Ludewig
citation:
ama: Yang H, Von Der Fecht Bartenbach J, Friml J, Lohmann J, Neuhäuser B, Ludewig
U. Auxin-modulated root growth inhibition in Arabidopsis thaliana seedlings with
ammonium as the sole nitrogen source. Functional Plant Biology. 2015;42(3):239-251.
doi:10.1071/FP14171
apa: Yang, H., Von Der Fecht Bartenbach, J., Friml, J., Lohmann, J., Neuhäuser,
B., & Ludewig, U. (2015). Auxin-modulated root growth inhibition in Arabidopsis
thaliana seedlings with ammonium as the sole nitrogen source. Functional Plant
Biology. CSIRO. https://doi.org/10.1071/FP14171
chicago: Yang, Huaiyu, Jenny Von Der Fecht Bartenbach, Jiří Friml, Jan Lohmann,
Benjamin Neuhäuser, and Uwe Ludewig. “Auxin-Modulated Root Growth Inhibition in
Arabidopsis Thaliana Seedlings with Ammonium as the Sole Nitrogen Source.” Functional
Plant Biology. CSIRO, 2015. https://doi.org/10.1071/FP14171.
ieee: H. Yang, J. Von Der Fecht Bartenbach, J. Friml, J. Lohmann, B. Neuhäuser,
and U. Ludewig, “Auxin-modulated root growth inhibition in Arabidopsis thaliana
seedlings with ammonium as the sole nitrogen source,” Functional Plant Biology,
vol. 42, no. 3. CSIRO, pp. 239–251, 2015.
ista: Yang H, Von Der Fecht Bartenbach J, Friml J, Lohmann J, Neuhäuser B, Ludewig
U. 2015. Auxin-modulated root growth inhibition in Arabidopsis thaliana seedlings
with ammonium as the sole nitrogen source. Functional Plant Biology. 42(3), 239–251.
mla: Yang, Huaiyu, et al. “Auxin-Modulated Root Growth Inhibition in Arabidopsis
Thaliana Seedlings with Ammonium as the Sole Nitrogen Source.” Functional Plant
Biology, vol. 42, no. 3, CSIRO, 2015, pp. 239–51, doi:10.1071/FP14171.
short: H. Yang, J. Von Der Fecht Bartenbach, J. Friml, J. Lohmann, B. Neuhäuser,
U. Ludewig, Functional Plant Biology 42 (2015) 239–251.
date_created: 2018-12-11T11:52:34Z
date_published: 2015-03-01T00:00:00Z
date_updated: 2022-05-24T09:02:24Z
day: '01'
department:
- _id: JiFr
doi: 10.1071/FP14171
external_id:
pmid:
- '32480670'
intvolume: ' 42'
issue: '3'
language:
- iso: eng
month: '03'
oa_version: None
page: 239 - 251
pmid: 1
publication: Functional Plant Biology
publication_identifier:
issn:
- 1445-4408
publication_status: published
publisher: CSIRO
publist_id: '5639'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Auxin-modulated root growth inhibition in Arabidopsis thaliana seedlings with
ammonium as the sole nitrogen source
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 42
year: '2015'
...
---
_id: '1534'
abstract:
- lang: eng
text: PIN proteins are auxin export carriers that direct intercellular auxin flow
and in turn regulate many aspects of plant growth and development including responses
to environmental changes. The Arabidopsis R2R3-MYB transcription factor FOUR LIPS
(FLP) and its paralogue MYB88 regulate terminal divisions during stomatal development,
as well as female reproductive development and stress responses. Here we show
that FLP and MYB88 act redundantly but differentially in regulating the transcription
of PIN3 and PIN7 in gravity-sensing cells of primary and lateral roots. On the
one hand, FLP is involved in responses to gravity stimulation in primary roots,
whereas on the other, FLP and MYB88 function complementarily in establishing the
gravitropic set-point angles of lateral roots. Our results support a model in
which FLP and MYB88 expression specifically determines the temporal-spatial patterns
of PIN3 and PIN7 transcription that are closely associated with their preferential
functions during root responses to gravity.
article_number: '8822'
author:
- first_name: Hongzhe
full_name: Wang, Hongzhe
last_name: Wang
- first_name: Kezhen
full_name: Yang, Kezhen
last_name: Yang
- first_name: Junjie
full_name: Zou, Junjie
last_name: Zou
- first_name: Lingling
full_name: Zhu, Lingling
last_name: Zhu
- first_name: Zidian
full_name: Xie, Zidian
last_name: Xie
- first_name: Miyoterao
full_name: Morita, Miyoterao
last_name: Morita
- first_name: Masao
full_name: Tasaka, Masao
last_name: Tasaka
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Erich
full_name: Grotewold, Erich
last_name: Grotewold
- first_name: Tom
full_name: Beeckman, Tom
last_name: Beeckman
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Fred
full_name: Sack, Fred
last_name: Sack
- first_name: Jie
full_name: Le, Jie
last_name: Le
citation:
ama: Wang H, Yang K, Zou J, et al. Transcriptional regulation of PIN genes by FOUR
LIPS and MYB88 during Arabidopsis root gravitropism. Nature Communications.
2015;6. doi:10.1038/ncomms9822
apa: Wang, H., Yang, K., Zou, J., Zhu, L., Xie, Z., Morita, M., … Le, J. (2015).
Transcriptional regulation of PIN genes by FOUR LIPS and MYB88 during Arabidopsis
root gravitropism. Nature Communications. Nature Publishing Group. https://doi.org/10.1038/ncomms9822
chicago: Wang, Hongzhe, Kezhen Yang, Junjie Zou, Lingling Zhu, Zidian Xie, Miyoterao
Morita, Masao Tasaka, et al. “Transcriptional Regulation of PIN Genes by FOUR
LIPS and MYB88 during Arabidopsis Root Gravitropism.” Nature Communications.
Nature Publishing Group, 2015. https://doi.org/10.1038/ncomms9822.
ieee: H. Wang et al., “Transcriptional regulation of PIN genes by FOUR LIPS
and MYB88 during Arabidopsis root gravitropism,” Nature Communications,
vol. 6. Nature Publishing Group, 2015.
ista: Wang H, Yang K, Zou J, Zhu L, Xie Z, Morita M, Tasaka M, Friml J, Grotewold
E, Beeckman T, Vanneste S, Sack F, Le J. 2015. Transcriptional regulation of PIN
genes by FOUR LIPS and MYB88 during Arabidopsis root gravitropism. Nature Communications.
6, 8822.
mla: Wang, Hongzhe, et al. “Transcriptional Regulation of PIN Genes by FOUR LIPS
and MYB88 during Arabidopsis Root Gravitropism.” Nature Communications,
vol. 6, 8822, Nature Publishing Group, 2015, doi:10.1038/ncomms9822.
short: H. Wang, K. Yang, J. Zou, L. Zhu, Z. Xie, M. Morita, M. Tasaka, J. Friml,
E. Grotewold, T. Beeckman, S. Vanneste, F. Sack, J. Le, Nature Communications
6 (2015).
date_created: 2018-12-11T11:52:34Z
date_published: 2015-11-18T00:00:00Z
date_updated: 2021-01-12T06:51:26Z
day: '18'
ddc:
- '570'
department:
- _id: JiFr
doi: 10.1038/ncomms9822
ec_funded: 1
file:
- access_level: open_access
checksum: 3c06735fc7cd7e482ca830cbd26001bf
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:17:07Z
date_updated: 2020-07-14T12:45:01Z
file_id: '5259'
file_name: IST-2016-485-v1+1_ncomms9822.pdf
file_size: 1852268
relation: main_file
file_date_updated: 2020-07-14T12:45:01Z
has_accepted_license: '1'
intvolume: ' 6'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Nature Communications
publication_status: published
publisher: Nature Publishing Group
publist_id: '5637'
pubrep_id: '485'
quality_controlled: '1'
scopus_import: 1
status: public
title: Transcriptional regulation of PIN genes by FOUR LIPS and MYB88 during Arabidopsis
root gravitropism
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 6
year: '2015'
...
---
_id: '1536'
abstract:
- lang: eng
text: Strigolactones, first discovered as germination stimulants for parasitic weeds
[1], are carotenoid-derived phytohormones that play major roles in inhibiting
lateral bud outgrowth and promoting plant-mycorrhizal symbiosis [2-4]. Furthermore,
strigolactones are involved in the regulation of lateral and adventitious root
development, root cell division [5, 6], secondary growth [7], and leaf senescence
[8]. Recently, we discovered the strigolactone transporter Petunia axillaris PLEIOTROPIC
DRUG RESISTANCE 1 (PaPDR1), which is required for efficient mycorrhizal colonization
and inhibition of lateral bud outgrowth [9]. However, how strigolactones are transported
through the plant remained unknown. Here we show that PaPDR1 exhibits a cell-type-specific
asymmetric localization in different root tissues. In root tips, PaPDR1 is co-expressed
with the strigolactone biosynthetic gene DAD1 (CCD8), and it is localized at the
apical membrane of root hypodermal cells, presumably mediating the shootward transport
of strigolactone. Above the root tip, in the hypodermal passage cells that form
gates for the entry of mycorrhizal fungi, PaPDR1 is present in the outer-lateral
membrane, compatible with its postulated function as strigolactone exporter from
root to soil. Transport studies are in line with our localization studies since
(1) a papdr1 mutant displays impaired transport of strigolactones out of the root
tip to the shoot as well as into the rhizosphere and (2) DAD1 expression and PIN1/PIN2
levels change in plants deregulated for PDR1 expression, suggestive of variations
in endogenous strigolactone contents. In conclusion, our results indicate that
the polar localizations of PaPDR1 mediate directional shootward strigolactone
transport as well as localized exudation into the soil.
acknowledgement: "This work was funded by a grant of the Swiss National Foundation
to E.M.\r\nWe thank Dr. José María Mateos (University of Zurich) for providing us
with the vibratome, Prof. Dolf Weijers (Wageningen University, the Netherlands)
for shipping us his set of ligation-independent cloning vectors, Prof. Bruno Humbel
(University of Lausanne) for suggestions on GFP-PDR1 detection, and Dr. Undine Krügel
(University of Zurich) and Prof. Michal Jasinski (Polish Academy of Science) for
hints on protein quantification."
author:
- first_name: Joëlle
full_name: Sasse, Joëlle
last_name: Sasse
- first_name: Sibu
full_name: Simon, Sibu
id: 4542EF9A-F248-11E8-B48F-1D18A9856A87
last_name: Simon
orcid: 0000-0002-1998-6741
- first_name: Christian
full_name: Gübeli, Christian
last_name: Gübeli
- first_name: Guowei
full_name: Liu, Guowei
last_name: Liu
- first_name: Xi
full_name: Cheng, Xi
last_name: Cheng
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Harro
full_name: Bouwmeester, Harro
last_name: Bouwmeester
- first_name: Enrico
full_name: Martinoia, Enrico
last_name: Martinoia
- first_name: Lorenzo
full_name: Borghi, Lorenzo
last_name: Borghi
citation:
ama: Sasse J, Simon S, Gübeli C, et al. Asymmetric localizations of the ABC transporter
PaPDR1 trace paths of directional strigolactone transport. Current Biology.
2015;25(5):647-655. doi:10.1016/j.cub.2015.01.015
apa: Sasse, J., Simon, S., Gübeli, C., Liu, G., Cheng, X., Friml, J., … Borghi,
L. (2015). Asymmetric localizations of the ABC transporter PaPDR1 trace paths
of directional strigolactone transport. Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2015.01.015
chicago: Sasse, Joëlle, Sibu Simon, Christian Gübeli, Guowei Liu, Xi Cheng, Jiří
Friml, Harro Bouwmeester, Enrico Martinoia, and Lorenzo Borghi. “Asymmetric Localizations
of the ABC Transporter PaPDR1 Trace Paths of Directional Strigolactone Transport.”
Current Biology. Cell Press, 2015. https://doi.org/10.1016/j.cub.2015.01.015.
ieee: J. Sasse et al., “Asymmetric localizations of the ABC transporter PaPDR1
trace paths of directional strigolactone transport,” Current Biology, vol.
25, no. 5. Cell Press, pp. 647–655, 2015.
ista: Sasse J, Simon S, Gübeli C, Liu G, Cheng X, Friml J, Bouwmeester H, Martinoia
E, Borghi L. 2015. Asymmetric localizations of the ABC transporter PaPDR1 trace
paths of directional strigolactone transport. Current Biology. 25(5), 647–655.
mla: Sasse, Joëlle, et al. “Asymmetric Localizations of the ABC Transporter PaPDR1
Trace Paths of Directional Strigolactone Transport.” Current Biology, vol.
25, no. 5, Cell Press, 2015, pp. 647–55, doi:10.1016/j.cub.2015.01.015.
short: J. Sasse, S. Simon, C. Gübeli, G. Liu, X. Cheng, J. Friml, H. Bouwmeester,
E. Martinoia, L. Borghi, Current Biology 25 (2015) 647–655.
date_created: 2018-12-11T11:52:35Z
date_published: 2015-02-12T00:00:00Z
date_updated: 2021-01-12T06:51:27Z
day: '12'
department:
- _id: JiFr
doi: 10.1016/j.cub.2015.01.015
intvolume: ' 25'
issue: '5'
language:
- iso: eng
month: '02'
oa_version: None
page: 647 - 655
publication: Current Biology
publication_status: published
publisher: Cell Press
publist_id: '5635'
quality_controlled: '1'
scopus_import: 1
status: public
title: Asymmetric localizations of the ABC transporter PaPDR1 trace paths of directional
strigolactone transport
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 25
year: '2015'
...
---
_id: '1543'
abstract:
- lang: eng
text: A plethora of diverse programmed cell death (PCD) processes has been described
in living organisms. In animals and plants, different forms of PCD play crucial
roles in development, immunity, and responses to the environment. While the molecular
control of some animal PCD forms such as apoptosis is known in great detail, we
still know comparatively little about the regulation of the diverse types of plant
PCD. In part, this deficiency in molecular understanding is caused by the lack
of reliable reporters to detect PCD processes. Here, we addressed this issue by
using a combination of bioinformatics approaches to identify commonly regulated
genes during diverse plant PCD processes in Arabidopsis (Arabidopsis thaliana).
Our results indicate that the transcriptional signatures of developmentally controlled
cell death are largely distinct from the ones associated with environmentally
induced cell death. Moreover, different cases of developmental PCD share a set
of cell death-associated genes. Most of these genes are evolutionary conserved
within the green plant lineage, arguing for an evolutionary conserved core machinery
of developmental PCD. Based on this information, we established an array of specific
promoter-reporter lines for developmental PCD in Arabidopsis. These PCD indicators
represent a powerful resource that can be used in addition to established morphological
and biochemical methods to detect and analyze PCD processes in vivo and in planta.
author:
- first_name: Yadira
full_name: Olvera Carrillo, Yadira
last_name: Olvera Carrillo
- first_name: Michiel
full_name: Van Bel, Michiel
last_name: Van Bel
- first_name: Tom
full_name: Van Hautegem, Tom
last_name: Van Hautegem
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: Marlies
full_name: Huysmans, Marlies
last_name: Huysmans
- first_name: Mária
full_name: Šimášková, Mária
last_name: Šimášková
- first_name: Matthias
full_name: Van Durme, Matthias
last_name: Van Durme
- first_name: Pierre
full_name: Buscaill, Pierre
last_name: Buscaill
- first_name: Susana
full_name: Rivas, Susana
last_name: Rivas
- first_name: Núria
full_name: Coll, Núria
last_name: Coll
- first_name: Frederik
full_name: Coppens, Frederik
last_name: Coppens
- first_name: Steven
full_name: Maere, Steven
last_name: Maere
- first_name: Moritz
full_name: Nowack, Moritz
last_name: Nowack
citation:
ama: Olvera Carrillo Y, Van Bel M, Van Hautegem T, et al. A conserved core of programmed
cell death indicator genes discriminates developmentally and environmentally induced
programmed cell death in plants. Plant Physiology. 2015;169(4):2684-2699.
doi:10.1104/pp.15.00769
apa: Olvera Carrillo, Y., Van Bel, M., Van Hautegem, T., Fendrych, M., Huysmans,
M., Šimášková, M., … Nowack, M. (2015). A conserved core of programmed cell death
indicator genes discriminates developmentally and environmentally induced programmed
cell death in plants. Plant Physiology. American Society of Plant Biologists.
https://doi.org/10.1104/pp.15.00769
chicago: Olvera Carrillo, Yadira, Michiel Van Bel, Tom Van Hautegem, Matyas Fendrych,
Marlies Huysmans, Mária Šimášková, Matthias Van Durme, et al. “A Conserved Core
of Programmed Cell Death Indicator Genes Discriminates Developmentally and Environmentally
Induced Programmed Cell Death in Plants.” Plant Physiology. American Society
of Plant Biologists, 2015. https://doi.org/10.1104/pp.15.00769.
ieee: Y. Olvera Carrillo et al., “A conserved core of programmed cell death
indicator genes discriminates developmentally and environmentally induced programmed
cell death in plants,” Plant Physiology, vol. 169, no. 4. American Society
of Plant Biologists, pp. 2684–2699, 2015.
ista: Olvera Carrillo Y, Van Bel M, Van Hautegem T, Fendrych M, Huysmans M, Šimášková
M, Van Durme M, Buscaill P, Rivas S, Coll N, Coppens F, Maere S, Nowack M. 2015.
A conserved core of programmed cell death indicator genes discriminates developmentally
and environmentally induced programmed cell death in plants. Plant Physiology.
169(4), 2684–2699.
mla: Olvera Carrillo, Yadira, et al. “A Conserved Core of Programmed Cell Death
Indicator Genes Discriminates Developmentally and Environmentally Induced Programmed
Cell Death in Plants.” Plant Physiology, vol. 169, no. 4, American Society
of Plant Biologists, 2015, pp. 2684–99, doi:10.1104/pp.15.00769.
short: Y. Olvera Carrillo, M. Van Bel, T. Van Hautegem, M. Fendrych, M. Huysmans,
M. Šimášková, M. Van Durme, P. Buscaill, S. Rivas, N. Coll, F. Coppens, S. Maere,
M. Nowack, Plant Physiology 169 (2015) 2684–2699.
date_created: 2018-12-11T11:52:38Z
date_published: 2015-12-01T00:00:00Z
date_updated: 2021-01-12T06:51:30Z
day: '01'
department:
- _id: JiFr
doi: 10.1104/pp.15.00769
intvolume: ' 169'
issue: '4'
language:
- iso: eng
month: '12'
oa_version: None
page: 2684 - 2699
publication: Plant Physiology
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '5628'
scopus_import: 1
status: public
title: A conserved core of programmed cell death indicator genes discriminates developmentally
and environmentally induced programmed cell death in plants
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 169
year: '2015'
...
---
_id: '1556'
abstract:
- lang: eng
text: The elongator complex subunit 2 (ELP2) protein, one subunit of an evolutionarily
conserved histone acetyltransferase complex, has been shown to participate in
leaf patterning, plant immune and abiotic stress responses in Arabidopsis thaliana.
Here, its role in root development was explored. Compared to the wild type, the
elp2 mutant exhibited an accelerated differentiation of its root stem cells and
cell division was more active in its quiescent centre (QC). The key transcription
factors responsible for maintaining root stem cell and QC identity, such as AP2
transcription factors PLT1 (PLETHORA1) and PLT2 (PLETHORA2), GRAS transcription
factors such as SCR (SCARECROW) and SHR (SHORT ROOT) and WUSCHEL-RELATED HOMEOBOX5
transcription factor WOX5, were all strongly down-regulated in the mutant. On
the other hand, expression of the G2/M transition activator CYCB1 was substantially
induced in elp2. The auxin efflux transporters PIN1 and PIN2 showed decreased
protein levels and PIN1 also displayed mild polarity alterations in elp2, which
resulted in a reduced auxin content in the root tip. Either the acetylation or
methylation level of each of these genes differed between the mutant and the wild
type, suggesting that the ELP2 regulation of root development involves the epigenetic
modification of a range of transcription factors and other developmental regulators.
author:
- first_name: Yuebin
full_name: Jia, Yuebin
last_name: Jia
- first_name: Huiyu
full_name: Tian, Huiyu
last_name: Tian
- first_name: Hongjiang
full_name: Li, Hongjiang
id: 33CA54A6-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0001-5039-9660
- first_name: Qianqian
full_name: Yu, Qianqian
last_name: Yu
- first_name: Lei
full_name: Wang, Lei
last_name: Wang
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Zhaojun
full_name: Ding, Zhaojun
last_name: Ding
citation:
ama: Jia Y, Tian H, Li H, et al. The Arabidopsis thaliana elongator complex subunit
2 epigenetically affects root development. Journal of Experimental Botany.
2015;66(15):4631-4642. doi:10.1093/jxb/erv230
apa: Jia, Y., Tian, H., Li, H., Yu, Q., Wang, L., Friml, J., & Ding, Z. (2015).
The Arabidopsis thaliana elongator complex subunit 2 epigenetically affects root
development. Journal of Experimental Botany. Oxford University Press. https://doi.org/10.1093/jxb/erv230
chicago: Jia, Yuebin, Huiyu Tian, Hongjiang Li, Qianqian Yu, Lei Wang, Jiří Friml,
and Zhaojun Ding. “The Arabidopsis Thaliana Elongator Complex Subunit 2 Epigenetically
Affects Root Development.” Journal of Experimental Botany. Oxford University
Press, 2015. https://doi.org/10.1093/jxb/erv230.
ieee: Y. Jia et al., “The Arabidopsis thaliana elongator complex subunit
2 epigenetically affects root development,” Journal of Experimental Botany,
vol. 66, no. 15. Oxford University Press, pp. 4631–4642, 2015.
ista: Jia Y, Tian H, Li H, Yu Q, Wang L, Friml J, Ding Z. 2015. The Arabidopsis
thaliana elongator complex subunit 2 epigenetically affects root development.
Journal of Experimental Botany. 66(15), 4631–4642.
mla: Jia, Yuebin, et al. “The Arabidopsis Thaliana Elongator Complex Subunit 2 Epigenetically
Affects Root Development.” Journal of Experimental Botany, vol. 66, no.
15, Oxford University Press, 2015, pp. 4631–42, doi:10.1093/jxb/erv230.
short: Y. Jia, H. Tian, H. Li, Q. Yu, L. Wang, J. Friml, Z. Ding, Journal of Experimental
Botany 66 (2015) 4631–4642.
date_created: 2018-12-11T11:52:42Z
date_published: 2015-08-01T00:00:00Z
date_updated: 2021-01-12T06:51:35Z
day: '01'
ddc:
- '570'
department:
- _id: JiFr
doi: 10.1093/jxb/erv230
file:
- access_level: open_access
checksum: 257919be0ce3d306185d3891ad7acf39
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:14:02Z
date_updated: 2020-07-14T12:45:02Z
file_id: '5051'
file_name: IST-2016-480-v1+1_J._Exp._Bot.-2015-Jia-4631-42.pdf
file_size: 7753043
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file_date_updated: 2020-07-14T12:45:02Z
has_accepted_license: '1'
intvolume: ' 66'
issue: '15'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
page: 4631 - 4642
publication: Journal of Experimental Botany
publication_status: published
publisher: Oxford University Press
publist_id: '5615'
pubrep_id: '480'
quality_controlled: '1'
scopus_import: 1
status: public
title: The Arabidopsis thaliana elongator complex subunit 2 epigenetically affects
root development
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 66
year: '2015'
...
---
_id: '1558'
abstract:
- lang: eng
text: CyclophilinAis a conserved peptidyl-prolyl cis-trans isomerase (PPIase) best
known as the cellular receptor of the immunosuppressant cyclosporine A. Despite
significant effort, evidence of developmental functions of cyclophilin A in non-plant
systems has remained obscure. Mutations in a tomato (Solanum lycopersicum) cyclophilin
A ortholog, DIAGEOTROPICA (DGT), have been shown to abolish the organogenesis
of lateral roots; however, a mechanistic explanation of the phenotype is lacking.
Here, we show that the dgt mutant lacks auxin maxima relevant to priming and specification
of lateral root founder cells. DGT is expressed in shoot and root, and localizes
to both the nucleus and cytoplasm during lateral root organogenesis. Mutation
of ENTIRE/ IAA9, a member of the auxin-responsive Aux/IAA protein family of transcriptional
repressors, partially restores the inability of dgt to initiate lateral root primordia
but not the primordia outgrowth. By comparison, grafting of a wild-type scion
restores the process of lateral root formation, consistent with participation
of a mobile signal. Antibodies do not detect movement of the DGT protein into
the dgt rootstock; however, experiments with radiolabeled auxin and an auxin-specific
microelectrode demonstrate abnormal auxin fluxes. Functional studies of DGT in
heterologous yeast and tobacco-leaf auxin-transport systems demonstrate that DGT
negatively regulates PIN-FORMED (PIN) auxin efflux transporters by affecting their
plasma membrane localization. Studies in tomato support complex effects of the
dgt mutation on PIN expression level, expression domain and plasma membrane localization.
Our data demonstrate that DGT regulates auxin transport in lateral root formation.
author:
- first_name: Maria
full_name: Ivanchenko, Maria
last_name: Ivanchenko
- first_name: Jinsheng
full_name: Zhu, Jinsheng
last_name: Zhu
- first_name: Bangjun
full_name: Wang, Bangjun
last_name: Wang
- first_name: Eva
full_name: Medvecka, Eva
id: 298814E2-F248-11E8-B48F-1D18A9856A87
last_name: Medvecka
- first_name: Yunlong
full_name: Du, Yunlong
last_name: Du
- first_name: Elisa
full_name: Azzarello, Elisa
last_name: Azzarello
- first_name: Stefano
full_name: Mancuso, Stefano
last_name: Mancuso
- first_name: Molly
full_name: Megraw, Molly
last_name: Megraw
- first_name: Sergei
full_name: Filichkin, Sergei
last_name: Filichkin
- first_name: Joseph
full_name: Dubrovsky, Joseph
last_name: Dubrovsky
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Markus
full_name: Geisler, Markus
last_name: Geisler
citation:
ama: Ivanchenko M, Zhu J, Wang B, et al. The cyclophilin a DIAGEOTROPICA gene affects
auxin transport in both root and shoot to control lateral root formation. Development.
2015;142(4):712-721. doi:10.1242/dev.113225
apa: Ivanchenko, M., Zhu, J., Wang, B., Medvecka, E., Du, Y., Azzarello, E., … Geisler,
M. (2015). The cyclophilin a DIAGEOTROPICA gene affects auxin transport in both
root and shoot to control lateral root formation. Development. Company
of Biologists. https://doi.org/10.1242/dev.113225
chicago: Ivanchenko, Maria, Jinsheng Zhu, Bangjun Wang, Eva Medvecka, Yunlong Du,
Elisa Azzarello, Stefano Mancuso, et al. “The Cyclophilin a DIAGEOTROPICA Gene
Affects Auxin Transport in Both Root and Shoot to Control Lateral Root Formation.”
Development. Company of Biologists, 2015. https://doi.org/10.1242/dev.113225.
ieee: M. Ivanchenko et al., “The cyclophilin a DIAGEOTROPICA gene affects
auxin transport in both root and shoot to control lateral root formation,” Development,
vol. 142, no. 4. Company of Biologists, pp. 712–721, 2015.
ista: Ivanchenko M, Zhu J, Wang B, Medvecka E, Du Y, Azzarello E, Mancuso S, Megraw
M, Filichkin S, Dubrovsky J, Friml J, Geisler M. 2015. The cyclophilin a DIAGEOTROPICA
gene affects auxin transport in both root and shoot to control lateral root formation.
Development. 142(4), 712–721.
mla: Ivanchenko, Maria, et al. “The Cyclophilin a DIAGEOTROPICA Gene Affects Auxin
Transport in Both Root and Shoot to Control Lateral Root Formation.” Development,
vol. 142, no. 4, Company of Biologists, 2015, pp. 712–21, doi:10.1242/dev.113225.
short: M. Ivanchenko, J. Zhu, B. Wang, E. Medvecka, Y. Du, E. Azzarello, S. Mancuso,
M. Megraw, S. Filichkin, J. Dubrovsky, J. Friml, M. Geisler, Development 142 (2015)
712–721.
date_created: 2018-12-11T11:52:42Z
date_published: 2015-02-15T00:00:00Z
date_updated: 2021-01-12T06:51:35Z
day: '15'
department:
- _id: JiFr
doi: 10.1242/dev.113225
intvolume: ' 142'
issue: '4'
language:
- iso: eng
month: '02'
oa_version: None
page: 712 - 721
publication: Development
publication_status: published
publisher: Company of Biologists
publist_id: '5613'
quality_controlled: '1'
scopus_import: 1
status: public
title: The cyclophilin a DIAGEOTROPICA gene affects auxin transport in both root and
shoot to control lateral root formation
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 142
year: '2015'
...
---
_id: '1554'
abstract:
- lang: eng
text: The visualization of hormonal signaling input and output is key to understanding
how multicellular development is regulated. The plant signaling molecule auxin
triggers many growth and developmental responses, but current tools lack the sensitivity
or precision to visualize these. We developed a set of fluorescent reporters that
allow sensitive and semiquantitative readout of auxin responses at cellular resolution
in Arabidopsis thaliana. These generic tools are suitable for any transformable
plant species.
author:
- first_name: Cheyang
full_name: Liao, Cheyang
last_name: Liao
- first_name: Wouter
full_name: Smet, Wouter
last_name: Smet
- first_name: Géraldine
full_name: Brunoud, Géraldine
last_name: Brunoud
- first_name: Saiko
full_name: Yoshida, Saiko
id: 2E46069C-F248-11E8-B48F-1D18A9856A87
last_name: Yoshida
- first_name: Teva
full_name: Vernoux, Teva
last_name: Vernoux
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
citation:
ama: Liao C, Smet W, Brunoud G, Yoshida S, Vernoux T, Weijers D. Reporters for sensitive
and quantitative measurement of auxin response. Nature Methods. 2015;12(3):207-210.
doi:10.1038/nmeth.3279
apa: Liao, C., Smet, W., Brunoud, G., Yoshida, S., Vernoux, T., & Weijers, D.
(2015). Reporters for sensitive and quantitative measurement of auxin response.
Nature Methods. Nature Publishing Group. https://doi.org/10.1038/nmeth.3279
chicago: Liao, Cheyang, Wouter Smet, Géraldine Brunoud, Saiko Yoshida, Teva Vernoux,
and Dolf Weijers. “Reporters for Sensitive and Quantitative Measurement of Auxin
Response.” Nature Methods. Nature Publishing Group, 2015. https://doi.org/10.1038/nmeth.3279.
ieee: C. Liao, W. Smet, G. Brunoud, S. Yoshida, T. Vernoux, and D. Weijers, “Reporters
for sensitive and quantitative measurement of auxin response,” Nature Methods,
vol. 12, no. 3. Nature Publishing Group, pp. 207–210, 2015.
ista: Liao C, Smet W, Brunoud G, Yoshida S, Vernoux T, Weijers D. 2015. Reporters
for sensitive and quantitative measurement of auxin response. Nature Methods.
12(3), 207–210.
mla: Liao, Cheyang, et al. “Reporters for Sensitive and Quantitative Measurement
of Auxin Response.” Nature Methods, vol. 12, no. 3, Nature Publishing Group,
2015, pp. 207–10, doi:10.1038/nmeth.3279.
short: C. Liao, W. Smet, G. Brunoud, S. Yoshida, T. Vernoux, D. Weijers, Nature
Methods 12 (2015) 207–210.
date_created: 2018-12-11T11:52:41Z
date_published: 2015-02-26T00:00:00Z
date_updated: 2021-01-12T06:51:34Z
day: '26'
department:
- _id: JiFr
doi: 10.1038/nmeth.3279
external_id:
pmid:
- '25643149'
intvolume: ' 12'
issue: '3'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344836/
month: '02'
oa: 1
oa_version: Submitted Version
page: 207 - 210
pmid: 1
publication: Nature Methods
publication_status: published
publisher: Nature Publishing Group
publist_id: '5617'
quality_controlled: '1'
scopus_import: 1
status: public
title: Reporters for sensitive and quantitative measurement of auxin response
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 12
year: '2015'
...