---
_id: '8822'
abstract:
- lang: eng
text: "Self-organization is a hallmark of plant development manifested e.g. by intricate
leaf vein patterns, flexible formation of vasculature during organogenesis or
its regeneration following wounding. Spontaneously arising channels transporting
the phytohormone auxin, created by coordinated polar localizations of PIN-FORMED
1 (PIN1) auxin exporter, provide positional cues for these as well as other plant
patterning processes. To find regulators acting downstream of auxin and the TIR1/AFB
auxin signaling pathway essential for PIN1 coordinated polarization during auxin
canalization, we performed microarray experiments. Besides the known components
of general PIN polarity maintenance, such as PID and PIP5K kinases, we identified
and characterized a new regulator of auxin canalization, the transcription factor
WRKY DNA-BINDING PROTEIN 23 (WRKY23).\r\nNext, we designed a subsequent microarray
experiment to further uncover other molecular players, downstream of auxin-TIR1/AFB-WRKY23
involved in the regulation of auxin-mediated PIN repolarization. We identified
a novel and crucial part of the molecular machinery underlying auxin canalization.
The auxin-regulated malectin-type receptor-like kinase CAMEL and the associated
leucine-rich repeat receptor-like kinase CANAR target and directly phosphorylate
PIN auxin transporters. camel and canar mutants are impaired in PIN1 subcellular
trafficking and auxin-mediated repolarization leading to defects in auxin transport,
ultimately to leaf venation and vasculature regeneration defects. Our results
describe the CAMEL-CANAR receptor complex, which is required for auxin feed-back
on its own transport and thus for coordinated tissue polarization during auxin
canalization."
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Jakub
full_name: Hajny, Jakub
id: 4800CC20-F248-11E8-B48F-1D18A9856A87
last_name: Hajny
orcid: 0000-0003-2140-7195
citation:
ama: Hajny J. Identification and characterization of the molecular machinery of
auxin-dependent canalization during vasculature formation and regeneration. 2020.
doi:10.15479/AT:ISTA:8822
apa: Hajny, J. (2020). Identification and characterization of the molecular machinery
of auxin-dependent canalization during vasculature formation and regeneration.
Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:8822
chicago: Hajny, Jakub. “Identification and Characterization of the Molecular Machinery
of Auxin-Dependent Canalization during Vasculature Formation and Regeneration.”
Institute of Science and Technology Austria, 2020. https://doi.org/10.15479/AT:ISTA:8822.
ieee: J. Hajny, “Identification and characterization of the molecular machinery
of auxin-dependent canalization during vasculature formation and regeneration,”
Institute of Science and Technology Austria, 2020.
ista: Hajny J. 2020. Identification and characterization of the molecular machinery
of auxin-dependent canalization during vasculature formation and regeneration.
Institute of Science and Technology Austria.
mla: Hajny, Jakub. Identification and Characterization of the Molecular Machinery
of Auxin-Dependent Canalization during Vasculature Formation and Regeneration.
Institute of Science and Technology Austria, 2020, doi:10.15479/AT:ISTA:8822.
short: J. Hajny, Identification and Characterization of the Molecular Machinery
of Auxin-Dependent Canalization during Vasculature Formation and Regeneration,
Institute of Science and Technology Austria, 2020.
date_created: 2020-12-01T12:38:18Z
date_published: 2020-12-01T00:00:00Z
date_updated: 2023-09-19T10:39:33Z
day: '01'
ddc:
- '580'
degree_awarded: PhD
department:
- _id: JiFr
doi: 10.15479/AT:ISTA:8822
file:
- access_level: closed
checksum: 210a9675af5e4c78b0b56d920ac82866
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: jhajny
date_created: 2020-12-04T07:27:52Z
date_updated: 2021-07-16T22:30:03Z
embargo_to: open_access
file_id: '8919'
file_name: Jakub Hajný IST Austria final_JH.docx
file_size: 91279806
relation: source_file
- access_level: open_access
checksum: 1781385b4aa73eba89cc76c6172f71d2
content_type: application/pdf
creator: jhajny
date_created: 2020-12-09T15:04:41Z
date_updated: 2021-12-08T23:30:03Z
embargo: 2021-12-07
file_id: '8933'
file_name: Jakub Hajný IST Austria final_JH-merged without Science.pdf
file_size: 68707697
relation: main_file
file_date_updated: 2021-12-08T23:30:03Z
has_accepted_license: '1'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
page: '249'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '7427'
relation: part_of_dissertation
status: public
- id: '6260'
relation: part_of_dissertation
status: public
- id: '7500'
relation: part_of_dissertation
status: public
- id: '191'
relation: part_of_dissertation
status: public
- id: '449'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
title: Identification and characterization of the molecular machinery of auxin-dependent
canalization during vasculature formation and regeneration
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2020'
...
---
_id: '8986'
abstract:
- lang: eng
text: 'Flowering plants display the highest diversity among plant species and have
notably shaped terrestrial landscapes. Nonetheless, the evolutionary origin of
their unprecedented morphological complexity remains largely an enigma. Here,
we show that the coevolution of cis-regulatory and coding regions of PIN-FORMED
(PIN) auxin transporters confined their expression to certain cell types and directed
their subcellular localization to particular cell sides, which together enabled
dynamic auxin gradients across tissues critical to the complex architecture of
flowering plants. Extensive intraspecies and interspecies genetic complementation
experiments with PINs from green alga up to flowering plant lineages showed that
PIN genes underwent three subsequent, critical evolutionary innovations and thus
acquired a triple function to regulate the development of three essential components
of the flowering plant Arabidopsis: shoot/root, inflorescence, and floral organ.
Our work highlights the critical role of functional innovations within the PIN
gene family as essential prerequisites for the origin of flowering plants.'
acknowledgement: 'We thank C.Löhne (Botanic Gardens, University of Bonn) for providing
us with A. trichopoda. We would like to thank T.Han, A.Mally (IST, Austria), and
C.Hartinger (University of Oxford) for constructive comment and careful reading.
Funding: The research leading to these results has received funding from the European
Union’s Horizon 2020 Research and Innovation Programme (ERC grant agreement number
742985), Austrian Science Fund (FWF, grant number I 3630-B25), DOC Fellowship of
the Austrian Academy of Sciences, and IST Fellow program. '
article_number: eabc8895
article_processing_charge: No
article_type: original
author:
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: Lesia
full_name: Rodriguez Solovey, Lesia
id: 3922B506-F248-11E8-B48F-1D18A9856A87
last_name: Rodriguez Solovey
orcid: 0000-0002-7244-7237
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Xixi
full_name: Zhang, Xixi
id: 61A66458-47E9-11EA-85BA-8AEAAF14E49A
last_name: Zhang
orcid: 0000-0001-7048-4627
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Zhang Y, Rodriguez Solovey L, Li L, Zhang X, Friml J. Functional innovations
of PIN auxin transporters mark crucial evolutionary transitions during rise of
flowering plants. Science Advances. 2020;6(50). doi:10.1126/sciadv.abc8895
apa: Zhang, Y., Rodriguez Solovey, L., Li, L., Zhang, X., & Friml, J. (2020).
Functional innovations of PIN auxin transporters mark crucial evolutionary transitions
during rise of flowering plants. Science Advances. AAAS. https://doi.org/10.1126/sciadv.abc8895
chicago: Zhang, Yuzhou, Lesia Rodriguez Solovey, Lanxin Li, Xixi Zhang, and Jiří
Friml. “Functional Innovations of PIN Auxin Transporters Mark Crucial Evolutionary
Transitions during Rise of Flowering Plants.” Science Advances. AAAS, 2020.
https://doi.org/10.1126/sciadv.abc8895.
ieee: Y. Zhang, L. Rodriguez Solovey, L. Li, X. Zhang, and J. Friml, “Functional
innovations of PIN auxin transporters mark crucial evolutionary transitions during
rise of flowering plants,” Science Advances, vol. 6, no. 50. AAAS, 2020.
ista: Zhang Y, Rodriguez Solovey L, Li L, Zhang X, Friml J. 2020. Functional innovations
of PIN auxin transporters mark crucial evolutionary transitions during rise of
flowering plants. Science Advances. 6(50), eabc8895.
mla: Zhang, Yuzhou, et al. “Functional Innovations of PIN Auxin Transporters Mark
Crucial Evolutionary Transitions during Rise of Flowering Plants.” Science
Advances, vol. 6, no. 50, eabc8895, AAAS, 2020, doi:10.1126/sciadv.abc8895.
short: Y. Zhang, L. Rodriguez Solovey, L. Li, X. Zhang, J. Friml, Science Advances
6 (2020).
date_created: 2021-01-03T23:01:23Z
date_published: 2020-12-11T00:00:00Z
date_updated: 2024-03-18T23:30:45Z
day: '11'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1126/sciadv.abc8895
ec_funded: 1
external_id:
isi:
- '000599903600014'
pmid:
- '33310852'
file:
- access_level: open_access
checksum: 5ac2500b191c08ef6dab5327f40ff663
content_type: application/pdf
creator: dernst
date_created: 2021-01-07T12:44:33Z
date_updated: 2021-01-07T12:44:33Z
file_id: '8994'
file_name: 2020_ScienceAdvances_Zhang.pdf
file_size: 10578145
relation: main_file
success: 1
file_date_updated: 2021-01-07T12:44:33Z
has_accepted_license: '1'
intvolume: ' 6'
isi: 1
issue: '50'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 26B4D67E-B435-11E9-9278-68D0E5697425
grant_number: '25351'
name: 'A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated
Rapid Growth Inhibition in Arabidopsis Root'
publication: Science Advances
publication_identifier:
eissn:
- 2375-2548
publication_status: published
publisher: AAAS
quality_controlled: '1'
related_material:
record:
- id: '10083'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Functional innovations of PIN auxin transporters mark crucial evolutionary
transitions during rise of flowering plants
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 6
year: '2020'
...
---
_id: '8283'
abstract:
- lang: eng
text: 'Drought and salt stress are the main environmental cues affecting the survival,
development, distribution, and yield of crops worldwide. MYB transcription factors
play a crucial role in plants’ biological processes, but the function of pineapple
MYB genes is still obscure. In this study, one of the pineapple MYB transcription
factors, AcoMYB4, was isolated and characterized. The results showed that AcoMYB4
is localized in the cell nucleus, and its expression is induced by low temperature,
drought, salt stress, and hormonal stimulation, especially by abscisic acid (ABA).
Overexpression of AcoMYB4 in rice and Arabidopsis enhanced plant sensitivity to
osmotic stress; it led to an increase in the number stomata on leaf surfaces and
lower germination rate under salt and drought stress. Furthermore, in AcoMYB4
OE lines, the membrane oxidation index, free proline, and soluble sugar contents
were decreased. In contrast, electrolyte leakage and malondialdehyde (MDA) content
increased significantly due to membrane injury, indicating higher sensitivity
to drought and salinity stresses. Besides the above, both the expression level
and activities of several antioxidant enzymes were decreased, indicating lower
antioxidant activity in AcoMYB4 transgenic plants. Moreover, under osmotic stress,
overexpression of AcoMYB4 inhibited ABA biosynthesis through a decrease in the
transcription of genes responsible for ABA synthesis (ABA1 and ABA2) and ABA signal
transduction factor ABI5. These results suggest that AcoMYB4 negatively regulates
osmotic stress by attenuating cellular ABA biosynthesis and signal transduction
pathways. '
acknowledgement: 'We would like to thank the reviewers for their helpful comments
on the original manuscript. '
article_number: '5272'
article_processing_charge: No
article_type: original
author:
- first_name: Huihuang
full_name: Chen, Huihuang
last_name: Chen
- first_name: Linyi
full_name: Lai, Linyi
last_name: Lai
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Liping
full_name: Liu, Liping
last_name: Liu
- first_name: Bello Hassan
full_name: Jakada, Bello Hassan
last_name: Jakada
- first_name: Youmei
full_name: Huang, Youmei
last_name: Huang
- first_name: Qing
full_name: He, Qing
last_name: He
- first_name: Mengnan
full_name: Chai, Mengnan
last_name: Chai
- first_name: Xiaoping
full_name: Niu, Xiaoping
last_name: Niu
- first_name: Yuan
full_name: Qin, Yuan
last_name: Qin
citation:
ama: Chen H, Lai L, Li L, et al. AcoMYB4, an Ananas comosus L. MYB transcription
factor, functions in osmotic stress through negative regulation of ABA signaling.
International Journal of Molecular Sciences. 2020;21(16). doi:10.3390/ijms21165727
apa: Chen, H., Lai, L., Li, L., Liu, L., Jakada, B. H., Huang, Y., … Qin, Y. (2020).
AcoMYB4, an Ananas comosus L. MYB transcription factor, functions in osmotic stress
through negative regulation of ABA signaling. International Journal of Molecular
Sciences. MDPI. https://doi.org/10.3390/ijms21165727
chicago: Chen, Huihuang, Linyi Lai, Lanxin Li, Liping Liu, Bello Hassan Jakada,
Youmei Huang, Qing He, Mengnan Chai, Xiaoping Niu, and Yuan Qin. “AcoMYB4, an
Ananas Comosus L. MYB Transcription Factor, Functions in Osmotic Stress through
Negative Regulation of ABA Signaling.” International Journal of Molecular Sciences.
MDPI, 2020. https://doi.org/10.3390/ijms21165727.
ieee: H. Chen et al., “AcoMYB4, an Ananas comosus L. MYB transcription factor,
functions in osmotic stress through negative regulation of ABA signaling,” International
Journal of Molecular Sciences, vol. 21, no. 16. MDPI, 2020.
ista: Chen H, Lai L, Li L, Liu L, Jakada BH, Huang Y, He Q, Chai M, Niu X, Qin Y.
2020. AcoMYB4, an Ananas comosus L. MYB transcription factor, functions in osmotic
stress through negative regulation of ABA signaling. International Journal of
Molecular Sciences. 21(16), 5272.
mla: Chen, Huihuang, et al. “AcoMYB4, an Ananas Comosus L. MYB Transcription Factor,
Functions in Osmotic Stress through Negative Regulation of ABA Signaling.” International
Journal of Molecular Sciences, vol. 21, no. 16, 5272, MDPI, 2020, doi:10.3390/ijms21165727.
short: H. Chen, L. Lai, L. Li, L. Liu, B.H. Jakada, Y. Huang, Q. He, M. Chai, X.
Niu, Y. Qin, International Journal of Molecular Sciences 21 (2020).
date_created: 2020-08-24T06:24:03Z
date_published: 2020-08-10T00:00:00Z
date_updated: 2024-03-18T23:30:45Z
day: '10'
ddc:
- '570'
department:
- _id: JiFr
doi: 10.3390/ijms21165727
external_id:
isi:
- '000565090300001'
pmid:
- '32785037'
file:
- access_level: open_access
checksum: 03b039244e6ae80580385fd9f577e2b2
content_type: application/pdf
creator: cziletti
date_created: 2020-08-25T09:53:50Z
date_updated: 2020-08-25T09:53:50Z
file_id: '8292'
file_name: 2020_IntMolecSciences_Chen.pdf
file_size: 5718755
relation: main_file
success: 1
file_date_updated: 2020-08-25T09:53:50Z
has_accepted_license: '1'
intvolume: ' 21'
isi: 1
issue: '16'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
publication: International Journal of Molecular Sciences
publication_identifier:
eissn:
- '14220067'
issn:
- '16616596'
publication_status: published
publisher: MDPI
quality_controlled: '1'
related_material:
record:
- id: '10083'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: AcoMYB4, an Ananas comosus L. MYB transcription factor, functions in osmotic
stress through negative regulation of ABA signaling
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 21
year: '2020'
...
---
_id: '8139'
abstract:
- lang: eng
text: 'Clathrin-mediated endocytosis (CME) is a crucial cellular process implicated
in many aspects of plant growth, development, intra- and inter-cellular signaling,
nutrient uptake and pathogen defense. Despite these significant roles, little
is known about the precise molecular details of how it functions in planta. In
order to facilitate the direct quantitative study of plant CME, here we review
current routinely used methods and present refined, standardized quantitative
imaging protocols which allow the detailed characterization of CME at multiple
scales in plant tissues. These include: (i) an efficient electron microscopy protocol
for the imaging of Arabidopsis CME vesicles in situ, thus providing a method for
the detailed characterization of the ultra-structure of clathrin-coated vesicles;
(ii) a detailed protocol and analysis for quantitative live-cell fluorescence
microscopy to precisely examine the temporal interplay of endocytosis components
during single CME events; (iii) a semi-automated analysis to allow the quantitative
characterization of global internalization of cargos in whole plant tissues; and
(iv) an overview and validation of useful genetic and pharmacological tools to
interrogate the molecular mechanisms and function of CME in intact plant samples.'
acknowledged_ssus:
- _id: EM-Fac
- _id: Bio
acknowledgement: "This paper is dedicated to the memory of Christien Merrifield. He
pioneered quantitative\r\nimaging approaches in mammalian CME and his mentorship
inspired the development of all\r\nthe analysis methods presented here. His joy
in research, pure scientific curiosity and\r\nmicroscopy excellence remain a constant
inspiration. We thank Daniel Van Damme for gifting\r\nus the CLC2-GFP x TPLATE-TagRFP
plants used in this manuscript. We further thank the\r\nScientific Service Units
at IST Austria; specifically, the Electron Microscopy Facility for\r\ntechnical
assistance (in particular Vanessa Zheden) and the BioImaging Facility BioImaging\r\nFacility
for access to equipment. "
article_number: jcs248062
article_processing_charge: No
article_type: original
author:
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Nataliia
full_name: Gnyliukh, Nataliia
id: 390C1120-F248-11E8-B48F-1D18A9856A87
last_name: Gnyliukh
orcid: 0000-0002-2198-0509
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Madhumitha
full_name: Narasimhan, Madhumitha
id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
last_name: Narasimhan
orcid: 0000-0002-8600-0671
- first_name: G
full_name: Vert, G
last_name: Vert
- first_name: SY
full_name: Bednarek, SY
last_name: Bednarek
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Johnson AJ, Gnyliukh N, Kaufmann W, et al. Experimental toolbox for quantitative
evaluation of clathrin-mediated endocytosis in the plant model Arabidopsis. Journal
of Cell Science. 2020;133(15). doi:10.1242/jcs.248062
apa: Johnson, A. J., Gnyliukh, N., Kaufmann, W., Narasimhan, M., Vert, G., Bednarek,
S., & Friml, J. (2020). Experimental toolbox for quantitative evaluation of
clathrin-mediated endocytosis in the plant model Arabidopsis. Journal of Cell
Science. The Company of Biologists. https://doi.org/10.1242/jcs.248062
chicago: Johnson, Alexander J, Nataliia Gnyliukh, Walter Kaufmann, Madhumitha Narasimhan,
G Vert, SY Bednarek, and Jiří Friml. “Experimental Toolbox for Quantitative Evaluation
of Clathrin-Mediated Endocytosis in the Plant Model Arabidopsis.” Journal of
Cell Science. The Company of Biologists, 2020. https://doi.org/10.1242/jcs.248062.
ieee: A. J. Johnson et al., “Experimental toolbox for quantitative evaluation
of clathrin-mediated endocytosis in the plant model Arabidopsis,” Journal of
Cell Science, vol. 133, no. 15. The Company of Biologists, 2020.
ista: Johnson AJ, Gnyliukh N, Kaufmann W, Narasimhan M, Vert G, Bednarek S, Friml
J. 2020. Experimental toolbox for quantitative evaluation of clathrin-mediated
endocytosis in the plant model Arabidopsis. Journal of Cell Science. 133(15),
jcs248062.
mla: Johnson, Alexander J., et al. “Experimental Toolbox for Quantitative Evaluation
of Clathrin-Mediated Endocytosis in the Plant Model Arabidopsis.” Journal of
Cell Science, vol. 133, no. 15, jcs248062, The Company of Biologists, 2020,
doi:10.1242/jcs.248062.
short: A.J. Johnson, N. Gnyliukh, W. Kaufmann, M. Narasimhan, G. Vert, S. Bednarek,
J. Friml, Journal of Cell Science 133 (2020).
date_created: 2020-07-21T08:58:19Z
date_published: 2020-08-06T00:00:00Z
date_updated: 2023-12-01T13:51:07Z
day: '06'
ddc:
- '575'
department:
- _id: JiFr
- _id: EM-Fac
doi: 10.1242/jcs.248062
ec_funded: 1
external_id:
isi:
- '000561047900021'
pmid:
- '32616560'
file:
- access_level: open_access
checksum: 2d11f79a0b4e0a380fb002b933da331a
content_type: application/pdf
creator: ajohnson
date_created: 2020-11-26T17:12:51Z
date_updated: 2021-08-08T22:30:03Z
embargo: 2021-08-07
file_id: '8815'
file_name: 2020 - Johnson - JSC - plant CME toolbox.pdf
file_size: 15150403
relation: main_file
file_date_updated: 2021-08-08T22:30:03Z
has_accepted_license: '1'
intvolume: ' 133'
isi: 1
issue: '15'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
publication: Journal of Cell Science
publication_identifier:
eissn:
- 1477-9137
issn:
- 0021-9533
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
related_material:
record:
- id: '14510'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Experimental toolbox for quantitative evaluation of clathrin-mediated endocytosis
in the plant model Arabidopsis
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 133
year: '2020'
...
---
_id: '5908'
abstract:
- lang: eng
text: The interorganelle communication mediated by membrane contact sites (MCSs)
is an evolutionary hallmark of eukaryotic cells. MCS connections enable the nonvesicular
exchange of information between organelles and allow them to coordinate responses
to changing cellular environments. In plants, the importance of MCS components
in the responses to environmental stress has been widely established, but the
molecular mechanisms regulating interorganelle connectivity during stress still
remain opaque. In this report, we use the model plant Arabidopsis thaliana to
show that ionic stress increases endoplasmic reticulum (ER)–plasma membrane (PM)
connectivity by promoting the cortical expansion of synaptotagmin 1 (SYT1)-enriched
ER–PM contact sites (S-EPCSs). We define differential roles for the cortical cytoskeleton
in the regulation of S-EPCS dynamics and ER–PM connectivity, and we identify the
accumulation of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] at the PM as
a molecular signal associated with the ER–PM connectivity changes. Our study highlights
the functional conservation of EPCS components and PM phosphoinositides as modulators
of ER–PM connectivity in eukaryotes, and uncovers unique aspects of the spatiotemporal
regulation of ER–PM connectivity in plants.
article_processing_charge: No
article_type: original
author:
- first_name: Eunkyoung
full_name: Lee, Eunkyoung
last_name: Lee
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Jessica
full_name: Pérez-Sancho, Jessica
last_name: Pérez-Sancho
- first_name: Francisco
full_name: Benitez-Fuente, Francisco
last_name: Benitez-Fuente
- first_name: Matthew
full_name: Strelau, Matthew
last_name: Strelau
- first_name: Alberto P.
full_name: Macho, Alberto P.
last_name: Macho
- first_name: Miguel A.
full_name: Botella, Miguel A.
last_name: Botella
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Abel
full_name: Rosado, Abel
last_name: Rosado
citation:
ama: Lee E, Vanneste S, Pérez-Sancho J, et al. Ionic stress enhances ER–PM connectivity
via phosphoinositide-associated SYT1 contact site expansion in Arabidopsis. Proceedings
of the National Academy of Sciences of the United States of America. 2019;116(4):1420-1429.
doi:10.1073/pnas.1818099116
apa: Lee, E., Vanneste, S., Pérez-Sancho, J., Benitez-Fuente, F., Strelau, M., Macho,
A. P., … Rosado, A. (2019). Ionic stress enhances ER–PM connectivity via phosphoinositide-associated
SYT1 contact site expansion in Arabidopsis. Proceedings of the National Academy
of Sciences of the United States of America. National Academy of Sciences.
https://doi.org/10.1073/pnas.1818099116
chicago: Lee, Eunkyoung, Steffen Vanneste, Jessica Pérez-Sancho, Francisco Benitez-Fuente,
Matthew Strelau, Alberto P. Macho, Miguel A. Botella, Jiří Friml, and Abel Rosado.
“Ionic Stress Enhances ER–PM Connectivity via Phosphoinositide-Associated SYT1
Contact Site Expansion in Arabidopsis.” Proceedings of the National Academy
of Sciences of the United States of America. National Academy of Sciences,
2019. https://doi.org/10.1073/pnas.1818099116.
ieee: E. Lee et al., “Ionic stress enhances ER–PM connectivity via phosphoinositide-associated
SYT1 contact site expansion in Arabidopsis,” Proceedings of the National Academy
of Sciences of the United States of America, vol. 116, no. 4. National Academy
of Sciences, pp. 1420–1429, 2019.
ista: Lee E, Vanneste S, Pérez-Sancho J, Benitez-Fuente F, Strelau M, Macho AP,
Botella MA, Friml J, Rosado A. 2019. Ionic stress enhances ER–PM connectivity
via phosphoinositide-associated SYT1 contact site expansion in Arabidopsis. Proceedings
of the National Academy of Sciences of the United States of America. 116(4), 1420–1429.
mla: Lee, Eunkyoung, et al. “Ionic Stress Enhances ER–PM Connectivity via Phosphoinositide-Associated
SYT1 Contact Site Expansion in Arabidopsis.” Proceedings of the National Academy
of Sciences of the United States of America, vol. 116, no. 4, National Academy
of Sciences, 2019, pp. 1420–29, doi:10.1073/pnas.1818099116.
short: E. Lee, S. Vanneste, J. Pérez-Sancho, F. Benitez-Fuente, M. Strelau, A.P.
Macho, M.A. Botella, J. Friml, A. Rosado, Proceedings of the National Academy
of Sciences of the United States of America 116 (2019) 1420–1429.
date_created: 2019-02-03T22:59:14Z
date_published: 2019-01-22T00:00:00Z
date_updated: 2023-08-24T14:31:09Z
day: '22'
department:
- _id: JiFr
doi: 10.1073/pnas.1818099116
external_id:
isi:
- '000456336100050'
pmid:
- '30610176'
intvolume: ' 116'
isi: 1
issue: '4'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1073/pnas.1818099116
month: '01'
oa: 1
oa_version: Published Version
page: 1420-1429
pmid: 1
publication: Proceedings of the National Academy of Sciences of the United States
of America
publication_status: published
publisher: National Academy of Sciences
quality_controlled: '1'
scopus_import: '1'
status: public
title: Ionic stress enhances ER–PM connectivity via phosphoinositide-associated SYT1
contact site expansion in Arabidopsis
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 116
year: '2019'
...
---
_id: '6023'
abstract:
- lang: eng
text: Multicellular development requires coordinated cell polarization relative
to body axes, and translation to oriented cell division 1–3 . In plants, it is
unknown how cell polarities are connected to organismal axes and translated to
division. Here, we identify Arabidopsis SOSEKI proteins that integrate apical–basal
and radial organismal axes to localize to polar cell edges. Localization does
not depend on tissue context, requires cell wall integrity and is defined by a
transferrable, protein-specific motif. A Domain of Unknown Function in SOSEKI
proteins resembles the DIX oligomerization domain in the animal Dishevelled polarity
regulator. The DIX-like domain self-interacts and is required for edge localization
and for influencing division orientation, together with a second domain that defines
the polar membrane domain. Our work shows that SOSEKI proteins locally interpret
global polarity cues and can influence cell division orientation. Furthermore,
this work reveals that, despite fundamental differences, cell polarity mechanisms
in plants and animals converge on a similar protein domain.
article_processing_charge: No
author:
- first_name: Saiko
full_name: Yoshida, Saiko
id: 2E46069C-F248-11E8-B48F-1D18A9856A87
last_name: Yoshida
- first_name: Alja
full_name: Van Der Schuren, Alja
last_name: Van Der Schuren
- first_name: Maritza
full_name: Van Dop, Maritza
last_name: Van Dop
- first_name: Luc
full_name: Van Galen, Luc
last_name: Van Galen
- first_name: Shunsuke
full_name: Saiga, Shunsuke
last_name: Saiga
- first_name: Milad
full_name: Adibi, Milad
last_name: Adibi
- first_name: Barbara
full_name: Möller, Barbara
last_name: Möller
- first_name: Colette A.
full_name: Ten Hove, Colette A.
last_name: Ten Hove
- first_name: Peter
full_name: Marhavy, Peter
id: 3F45B078-F248-11E8-B48F-1D18A9856A87
last_name: Marhavy
orcid: 0000-0001-5227-5741
- first_name: Richard
full_name: Smith, Richard
last_name: Smith
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
citation:
ama: Yoshida S, Van Der Schuren A, Van Dop M, et al. A SOSEKI-based coordinate system
interprets global polarity cues in arabidopsis. Nature Plants. 2019;5(2):160-166.
doi:10.1038/s41477-019-0363-6
apa: Yoshida, S., Van Der Schuren, A., Van Dop, M., Van Galen, L., Saiga, S., Adibi,
M., … Weijers, D. (2019). A SOSEKI-based coordinate system interprets global polarity
cues in arabidopsis. Nature Plants. Springer Nature. https://doi.org/10.1038/s41477-019-0363-6
chicago: Yoshida, Saiko, Alja Van Der Schuren, Maritza Van Dop, Luc Van Galen, Shunsuke
Saiga, Milad Adibi, Barbara Möller, et al. “A SOSEKI-Based Coordinate System Interprets
Global Polarity Cues in Arabidopsis.” Nature Plants. Springer Nature, 2019.
https://doi.org/10.1038/s41477-019-0363-6.
ieee: S. Yoshida et al., “A SOSEKI-based coordinate system interprets global
polarity cues in arabidopsis,” Nature Plants, vol. 5, no. 2. Springer Nature,
pp. 160–166, 2019.
ista: Yoshida S, Van Der Schuren A, Van Dop M, Van Galen L, Saiga S, Adibi M, Möller
B, Ten Hove CA, Marhavý P, Smith R, Friml J, Weijers D. 2019. A SOSEKI-based coordinate
system interprets global polarity cues in arabidopsis. Nature Plants. 5(2), 160–166.
mla: Yoshida, Saiko, et al. “A SOSEKI-Based Coordinate System Interprets Global
Polarity Cues in Arabidopsis.” Nature Plants, vol. 5, no. 2, Springer Nature,
2019, pp. 160–66, doi:10.1038/s41477-019-0363-6.
short: S. Yoshida, A. Van Der Schuren, M. Van Dop, L. Van Galen, S. Saiga, M. Adibi,
B. Möller, C.A. Ten Hove, P. Marhavý, R. Smith, J. Friml, D. Weijers, Nature Plants
5 (2019) 160–166.
date_created: 2019-02-17T22:59:21Z
date_published: 2019-02-08T00:00:00Z
date_updated: 2023-08-24T14:46:47Z
day: '08'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1038/s41477-019-0363-6
ec_funded: 1
external_id:
isi:
- '000460479600014'
intvolume: ' 5'
isi: 1
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.biorxiv.org/content/10.1101/479113v1.abstract
month: '02'
oa: 1
oa_version: Submitted Version
page: 160-166
project:
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
publication: Nature Plants
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: A SOSEKI-based coordinate system interprets global polarity cues in arabidopsis
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 5
year: '2019'
...
---
_id: '6104'
abstract:
- lang: eng
text: Abiotic stress poses constant challenges for plant survival and is a serious
problem for global agricultural productivity. On a molecular level, stress conditions
result in elevation of reactive oxygen species (ROS) production causing oxidative
stress associated with oxidation of proteins and nucleic acids as well as impairment
of membrane functions. Adaptation of root growth to ROS accumulation is facilitated
through modification of auxin and cytokinin hormone homeostasis. Here, we report
that in Arabidopsis root meristem, ROS-induced changes of auxin levels correspond
to decreased abundance of PIN auxin efflux carriers at the plasma membrane (PM).
Specifically, increase in H2O2 levels affects PIN2 endocytic recycling. We show
that the PIN2 intracellular trafficking during adaptation to oxidative stress
requires the function of the ADP-ribosylation factor (ARF)-guanine-nucleotide
exchange factor (GEF) BEN1, an actin-associated regulator of the trafficking from
the PM to early endosomes and, presumably, indirectly, trafficking to the vacuoles.
We propose that H2O2 levels affect the actin dynamics thus modulating ARF-GEF-dependent
trafficking of PIN2. This mechanism provides a way how root growth acclimates
to stress and adapts to a changing environment.
article_processing_charge: No
author:
- first_name: Marta
full_name: Zwiewka, Marta
last_name: Zwiewka
- first_name: Agnieszka
full_name: Bielach, Agnieszka
last_name: Bielach
- first_name: Prashanth
full_name: Tamizhselvan, Prashanth
last_name: Tamizhselvan
- first_name: Sharmila
full_name: Madhavan, Sharmila
last_name: Madhavan
- first_name: Eman Elrefaay
full_name: Ryad, Eman Elrefaay
last_name: Ryad
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Mónika
full_name: Hrtyan, Mónika
id: 45A71A74-F248-11E8-B48F-1D18A9856A87
last_name: Hrtyan
- first_name: Petre
full_name: Dobrev, Petre
last_name: Dobrev
- first_name: Radomira
full_name: Vanková, Radomira
last_name: Vanková
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Vanesa B.
full_name: Tognetti, Vanesa B.
last_name: Tognetti
citation:
ama: Zwiewka M, Bielach A, Tamizhselvan P, et al. Root adaptation to H2O2-induced
oxidative stress by ARF-GEF BEN1- and cytoskeleton-mediated PIN2 trafficking.
Plant and Cell Physiology. 2019;60(2):255-273. doi:10.1093/pcp/pcz001
apa: Zwiewka, M., Bielach, A., Tamizhselvan, P., Madhavan, S., Ryad, E. E., Tan,
S., … Tognetti, V. B. (2019). Root adaptation to H2O2-induced oxidative stress
by ARF-GEF BEN1- and cytoskeleton-mediated PIN2 trafficking. Plant and Cell
Physiology. Oxford University Press. https://doi.org/10.1093/pcp/pcz001
chicago: Zwiewka, Marta, Agnieszka Bielach, Prashanth Tamizhselvan, Sharmila Madhavan,
Eman Elrefaay Ryad, Shutang Tan, Mónika Hrtyan, et al. “Root Adaptation to H2O2-Induced
Oxidative Stress by ARF-GEF BEN1- and Cytoskeleton-Mediated PIN2 Trafficking.”
Plant and Cell Physiology. Oxford University Press, 2019. https://doi.org/10.1093/pcp/pcz001.
ieee: M. Zwiewka et al., “Root adaptation to H2O2-induced oxidative stress
by ARF-GEF BEN1- and cytoskeleton-mediated PIN2 trafficking,” Plant and Cell
Physiology, vol. 60, no. 2. Oxford University Press, pp. 255–273, 2019.
ista: Zwiewka M, Bielach A, Tamizhselvan P, Madhavan S, Ryad EE, Tan S, Hrtyan M,
Dobrev P, Vanková R, Friml J, Tognetti VB. 2019. Root adaptation to H2O2-induced
oxidative stress by ARF-GEF BEN1- and cytoskeleton-mediated PIN2 trafficking.
Plant and Cell Physiology. 60(2), 255–273.
mla: Zwiewka, Marta, et al. “Root Adaptation to H2O2-Induced Oxidative Stress by
ARF-GEF BEN1- and Cytoskeleton-Mediated PIN2 Trafficking.” Plant and Cell Physiology,
vol. 60, no. 2, Oxford University Press, 2019, pp. 255–73, doi:10.1093/pcp/pcz001.
short: M. Zwiewka, A. Bielach, P. Tamizhselvan, S. Madhavan, E.E. Ryad, S. Tan,
M. Hrtyan, P. Dobrev, R. Vanková, J. Friml, V.B. Tognetti, Plant and Cell Physiology
60 (2019) 255–273.
date_created: 2019-03-17T22:59:14Z
date_published: 2019-02-01T00:00:00Z
date_updated: 2023-08-25T08:05:28Z
day: '01'
department:
- _id: JiFr
doi: 10.1093/pcp/pcz001
external_id:
isi:
- '000459634300002'
pmid:
- '30668780'
intvolume: ' 60'
isi: 1
issue: '2'
language:
- iso: eng
month: '02'
oa_version: None
page: 255-273
pmid: 1
publication: Plant and Cell Physiology
publication_identifier:
eissn:
- 1471-9053
issn:
- 0032-0781
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Root adaptation to H2O2-induced oxidative stress by ARF-GEF BEN1- and cytoskeleton-mediated
PIN2 trafficking
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 60
year: '2019'
...
---
_id: '6262'
abstract:
- lang: eng
text: "Gravitropism is an adaptive response that orients plant growth parallel to
the gravity vector. Asymmetric\r\ndistribution of the phytohormone auxin is a
necessary prerequisite to the tropic bending both in roots and\r\nshoots. During
hypocotyl gravitropic response, the PIN3 auxin transporter polarizes within gravity-sensing\r\ncells
to redirect intercellular auxin fluxes. First gravity-induced PIN3 polarization
to the bottom cell mem-\r\nbranes leads to the auxin accumulation at the lower
side of the organ, initiating bending and, later, auxin\r\nfeedback-mediated repolarization
restores symmetric auxin distribution to terminate bending. Here, we per-\r\nformed
a forward genetic screen to identify regulators of both PIN3 polarization events
during gravitropic\r\nresponse. We searched for mutants with defective PIN3 polarizations
based on easy-to-score morphological\r\noutputs of decreased or increased gravity-induced
hypocotyl bending. We identified the number of\r\nhypocotyl reduced bending (hrb)
and hypocotyl hyperbending (hhb) mutants, revealing that reduced bending corre-\r\nlated
typically with defective gravity-induced PIN3 relocation whereas all analyzed
hhb mutants showed\r\ndefects in the second, auxin-mediated PIN3 relocation. Next-generation
sequencing-aided mutation map-\r\nping identified several candidate genes, including
SCARECROW and ACTIN2, revealing roles of endodermis\r\nspecification and actin
cytoskeleton in the respective gravity- and auxin-induced PIN polarization events.\r\nThe
hypocotyl gravitropism screen thus promises to provide novel insights into mechanisms
underlying cell\r\npolarity and plant adaptive development."
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Hana
full_name: Rakusová, Hana
last_name: Rakusová
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
- first_name: Petr
full_name: Valošek, Petr
id: 3CDB6F94-F248-11E8-B48F-1D18A9856A87
last_name: Valošek
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Rakusová H, Han H, Valošek P, Friml J. Genetic screen for factors mediating
PIN polarization in gravistimulated Arabidopsis thaliana hypocotyls. The Plant
Journal. 2019;98(6):1048-1059. doi:10.1111/tpj.14301
apa: Rakusová, H., Han, H., Valošek, P., & Friml, J. (2019). Genetic screen
for factors mediating PIN polarization in gravistimulated Arabidopsis thaliana
hypocotyls. The Plant Journal. Wiley. https://doi.org/10.1111/tpj.14301
chicago: Rakusová, Hana, Huibin Han, Petr Valošek, and Jiří Friml. “Genetic Screen
for Factors Mediating PIN Polarization in Gravistimulated Arabidopsis Thaliana
Hypocotyls.” The Plant Journal. Wiley, 2019. https://doi.org/10.1111/tpj.14301.
ieee: H. Rakusová, H. Han, P. Valošek, and J. Friml, “Genetic screen for factors
mediating PIN polarization in gravistimulated Arabidopsis thaliana hypocotyls,”
The Plant Journal, vol. 98, no. 6. Wiley, pp. 1048–1059, 2019.
ista: Rakusová H, Han H, Valošek P, Friml J. 2019. Genetic screen for factors mediating
PIN polarization in gravistimulated Arabidopsis thaliana hypocotyls. The Plant
Journal. 98(6), 1048–1059.
mla: Rakusová, Hana, et al. “Genetic Screen for Factors Mediating PIN Polarization
in Gravistimulated Arabidopsis Thaliana Hypocotyls.” The Plant Journal,
vol. 98, no. 6, Wiley, 2019, pp. 1048–59, doi:10.1111/tpj.14301.
short: H. Rakusová, H. Han, P. Valošek, J. Friml, The Plant Journal 98 (2019) 1048–1059.
date_created: 2019-04-09T08:46:44Z
date_published: 2019-06-01T00:00:00Z
date_updated: 2023-08-25T10:11:03Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/tpj.14301
ec_funded: 1
external_id:
isi:
- '000473644100008'
pmid:
- '30821050'
file:
- access_level: open_access
checksum: ad3b5e270b67ba2a45f894ce3be27920
content_type: application/pdf
creator: dernst
date_created: 2019-04-15T09:38:43Z
date_updated: 2020-07-14T12:47:25Z
file_id: '6304'
file_name: 2019_PlantJournal_Rakusov.pdf
file_size: 1383100
relation: main_file
file_date_updated: 2020-07-14T12:47:25Z
has_accepted_license: '1'
intvolume: ' 98'
isi: 1
issue: '6'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
page: 1048-1059
pmid: 1
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: The Plant Journal
publication_identifier:
eissn:
- 1365-313x
issn:
- 0960-7412
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Genetic screen for factors mediating PIN polarization in gravistimulated Arabidopsis
thaliana hypocotyls
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 98
year: '2019'
...
---
_id: '6261'
abstract:
- lang: eng
text: Nitrate regulation of root stem cell activity is auxin-dependent.
article_processing_charge: No
article_type: letter_note
author:
- first_name: Y
full_name: Wang, Y
last_name: Wang
- first_name: Z
full_name: Gong, Z
last_name: Gong
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: J
full_name: Zhang, J
last_name: Zhang
citation:
ama: Wang Y, Gong Z, Friml J, Zhang J. Nitrate modulates the differentiation of
root distal stem cells. Plant Physiology. 2019;180(1):22-25. doi:10.1104/pp.18.01305
apa: Wang, Y., Gong, Z., Friml, J., & Zhang, J. (2019). Nitrate modulates the
differentiation of root distal stem cells. Plant Physiology. ASPB. https://doi.org/10.1104/pp.18.01305
chicago: Wang, Y, Z Gong, Jiří Friml, and J Zhang. “Nitrate Modulates the Differentiation
of Root Distal Stem Cells.” Plant Physiology. ASPB, 2019. https://doi.org/10.1104/pp.18.01305.
ieee: Y. Wang, Z. Gong, J. Friml, and J. Zhang, “Nitrate modulates the differentiation
of root distal stem cells,” Plant Physiology, vol. 180, no. 1. ASPB, pp.
22–25, 2019.
ista: Wang Y, Gong Z, Friml J, Zhang J. 2019. Nitrate modulates the differentiation
of root distal stem cells. Plant Physiology. 180(1), 22–25.
mla: Wang, Y., et al. “Nitrate Modulates the Differentiation of Root Distal Stem
Cells.” Plant Physiology, vol. 180, no. 1, ASPB, 2019, pp. 22–25, doi:10.1104/pp.18.01305.
short: Y. Wang, Z. Gong, J. Friml, J. Zhang, Plant Physiology 180 (2019) 22–25.
date_created: 2019-04-09T08:46:17Z
date_published: 2019-05-01T00:00:00Z
date_updated: 2023-08-25T10:10:23Z
day: '01'
department:
- _id: JiFr
doi: 10.1104/pp.18.01305
external_id:
isi:
- '000466860800010'
pmid:
- '30787134'
intvolume: ' 180'
isi: 1
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1104/pp.18.01305
month: '05'
oa: 1
oa_version: Published Version
page: 22-25
pmid: 1
publication: Plant Physiology
publication_identifier:
eissn:
- 1532-2548
issn:
- 0032-0889
publication_status: published
publisher: ASPB
quality_controlled: '1'
scopus_import: '1'
status: public
title: Nitrate modulates the differentiation of root distal stem cells
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 180
year: '2019'
...
---
_id: '6504'
abstract:
- lang: eng
text: "Root gravitropism is one of the most important processes allowing plant adaptation
to the land environment. Auxin plays a central role in mediating root gravitropism,
but how auxin contributes to gravitational perception and the subsequent response
is still unclear.\r\n\r\nHere, we showed that the local auxin maximum/gradient
within the root apex, which is generated by the PIN directional auxin transporters,
regulates the expression of three key starch granule synthesis genes, SS4, PGM
and ADG1, which in turn influence the accumulation of starch granules that serve
as a statolith perceiving gravity.\r\n\r\nMoreover, using the cvxIAA‐ccvTIR1 system,
we also showed that TIR1‐mediated auxin signaling is required for starch granule
formation and gravitropic response within root tips. In addition, axr3 mutants
showed reduced auxin‐mediated starch granule accumulation and disruption of gravitropism
within the root apex.\r\n\r\nOur results indicate that auxin‐mediated statolith
production relies on the TIR1/AFB‐AXR3‐mediated auxin signaling pathway. In summary,
we propose a dual role for auxin in gravitropism: the regulation of both gravity
perception and response."
article_processing_charge: No
article_type: original
author:
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: P
full_name: He, P
last_name: He
- first_name: X
full_name: Ma, X
last_name: Ma
- first_name: Z
full_name: Yang, Z
last_name: Yang
- first_name: C
full_name: Pang, C
last_name: Pang
- first_name: J
full_name: Yu, J
last_name: Yu
- first_name: G
full_name: Wang, G
last_name: Wang
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: G
full_name: Xiao, G
last_name: Xiao
citation:
ama: Zhang Y, He P, Ma X, et al. Auxin-mediated statolith production for root gravitropism.
New Phytologist. 2019;224(2):761-774. doi:10.1111/nph.15932
apa: Zhang, Y., He, P., Ma, X., Yang, Z., Pang, C., Yu, J., … Xiao, G. (2019). Auxin-mediated
statolith production for root gravitropism. New Phytologist. Wiley. https://doi.org/10.1111/nph.15932
chicago: Zhang, Yuzhou, P He, X Ma, Z Yang, C Pang, J Yu, G Wang, Jiří Friml, and
G Xiao. “Auxin-Mediated Statolith Production for Root Gravitropism.” New Phytologist.
Wiley, 2019. https://doi.org/10.1111/nph.15932.
ieee: Y. Zhang et al., “Auxin-mediated statolith production for root gravitropism,”
New Phytologist, vol. 224, no. 2. Wiley, pp. 761–774, 2019.
ista: Zhang Y, He P, Ma X, Yang Z, Pang C, Yu J, Wang G, Friml J, Xiao G. 2019.
Auxin-mediated statolith production for root gravitropism. New Phytologist. 224(2),
761–774.
mla: Zhang, Yuzhou, et al. “Auxin-Mediated Statolith Production for Root Gravitropism.”
New Phytologist, vol. 224, no. 2, Wiley, 2019, pp. 761–74, doi:10.1111/nph.15932.
short: Y. Zhang, P. He, X. Ma, Z. Yang, C. Pang, J. Yu, G. Wang, J. Friml, G. Xiao,
New Phytologist 224 (2019) 761–774.
date_created: 2019-05-28T14:33:26Z
date_published: 2019-10-01T00:00:00Z
date_updated: 2023-08-28T08:40:13Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/nph.15932
external_id:
isi:
- '000487184200024'
pmid:
- '31111487'
file:
- access_level: open_access
checksum: 6488243334538f5c39099a701cbf76b9
content_type: application/pdf
creator: dernst
date_created: 2020-10-14T08:59:33Z
date_updated: 2020-10-14T08:59:33Z
file_id: '8661'
file_name: 2019_NewPhytologist_Zhang_accepted.pdf
file_size: 1099061
relation: main_file
success: 1
file_date_updated: 2020-10-14T08:59:33Z
has_accepted_license: '1'
intvolume: ' 224'
isi: 1
issue: '2'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Submitted Version
page: 761-774
pmid: 1
publication: New Phytologist
publication_identifier:
eissn:
- 1469-8137
issn:
- 0028-646x
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Auxin-mediated statolith production for root gravitropism
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 224
year: '2019'
...
---
_id: '6611'
abstract:
- lang: eng
text: 'Cell polarity is crucial for the coordinated development of all multicellular
organisms. In plants, this is exemplified by the PIN-FORMED (PIN) efflux carriers
of the phytohormone auxin: The polar subcellular localization of the PINs is instructive
to the directional intercellular auxin transport, and thus to a plethora of auxin-regulated
growth and developmental processes. Despite its importance, the regulation of
PIN polar subcellular localization remains poorly understood. Here, we have employed
advanced live-cell imaging techniques to study the roles of microtubules and actin
microfilaments in the establishment of apical polar localization of PIN2 in the
epidermis of the Arabidopsis root meristem. We report that apical PIN2 polarity
requires neither intact actin microfilaments nor microtubules, suggesting that
the primary spatial cue for polar PIN distribution is likely independent of cytoskeleton-guided
endomembrane trafficking.'
acknowledged_ssus:
- _id: Bio
article_number: '222'
article_processing_charge: No
author:
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Glanc M, Fendrych M, Friml J. PIN2 polarity establishment in arabidopsis in
the absence of an intact cytoskeleton. Biomolecules. 2019;9(6). doi:10.3390/biom9060222
apa: Glanc, M., Fendrych, M., & Friml, J. (2019). PIN2 polarity establishment
in arabidopsis in the absence of an intact cytoskeleton. Biomolecules.
MDPI. https://doi.org/10.3390/biom9060222
chicago: Glanc, Matous, Matyas Fendrych, and Jiří Friml. “PIN2 Polarity Establishment
in Arabidopsis in the Absence of an Intact Cytoskeleton.” Biomolecules.
MDPI, 2019. https://doi.org/10.3390/biom9060222.
ieee: M. Glanc, M. Fendrych, and J. Friml, “PIN2 polarity establishment in arabidopsis
in the absence of an intact cytoskeleton,” Biomolecules, vol. 9, no. 6.
MDPI, 2019.
ista: Glanc M, Fendrych M, Friml J. 2019. PIN2 polarity establishment in arabidopsis
in the absence of an intact cytoskeleton. Biomolecules. 9(6), 222.
mla: Glanc, Matous, et al. “PIN2 Polarity Establishment in Arabidopsis in the Absence
of an Intact Cytoskeleton.” Biomolecules, vol. 9, no. 6, 222, MDPI, 2019,
doi:10.3390/biom9060222.
short: M. Glanc, M. Fendrych, J. Friml, Biomolecules 9 (2019).
date_created: 2019-07-07T21:59:21Z
date_published: 2019-06-07T00:00:00Z
date_updated: 2023-08-28T12:30:24Z
day: '07'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.3390/biom9060222
ec_funded: 1
external_id:
isi:
- '000475301500018'
pmid:
- '31181636'
file:
- access_level: open_access
checksum: 1ce1bd36038fe5381057a1bcc6760083
content_type: application/pdf
creator: kschuh
date_created: 2019-07-08T15:46:32Z
date_updated: 2020-07-14T12:47:34Z
file_id: '6625'
file_name: biomolecules-2019-Matous.pdf
file_size: 1066773
relation: main_file
file_date_updated: 2020-07-14T12:47:34Z
has_accepted_license: '1'
intvolume: ' 9'
isi: 1
issue: '6'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Biomolecules
publication_status: published
publisher: MDPI
quality_controlled: '1'
scopus_import: '1'
status: public
title: PIN2 polarity establishment in arabidopsis in the absence of an intact cytoskeleton
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 9
year: '2019'
...
---
_id: '6778'
abstract:
- lang: eng
text: "An important adaptation during colonization of land by plants is gravitropic
growth of roots, which enabled roots to reach water and nutrients, and firmly
anchor plants in the ground. Here we provide insights into the evolution of an
efficient root gravitropic mechanism in the seed plants. Architectural innovation,
with gravity perception constrained in the root tips\r\nalong with a shootward
transport route for the phytohormone auxin, appeared only upon the emergence of
seed plants. Interspecies complementation and protein domain swapping revealed
functional innovations within the PIN family of auxin transporters leading to
the evolution of gravitropism-specific PINs. The unique apical/shootward subcellular
localization of PIN proteins is the major evolutionary innovation that connected
the anatomically separated sites of gravity perception and growth response via
the mobile auxin signal. We conclude that the crucial anatomical and functional
components emerged hand-in-hand to facilitate the evolution of fast gravitropic
response, which is one of the major adaptations of seed plants to dry land."
article_number: '3480'
article_processing_charge: No
article_type: original
author:
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: G
full_name: Xiao, G
last_name: Xiao
- first_name: X
full_name: Wang, X
last_name: Wang
- first_name: Xixi
full_name: Zhang, Xixi
id: 61A66458-47E9-11EA-85BA-8AEAAF14E49A
last_name: Zhang
orcid: 0000-0001-7048-4627
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Zhang Y, Xiao G, Wang X, Zhang X, Friml J. Evolution of fast root gravitropism
in seed plants. Nature Communications. 2019;10. doi:10.1038/s41467-019-11471-8
apa: Zhang, Y., Xiao, G., Wang, X., Zhang, X., & Friml, J. (2019). Evolution
of fast root gravitropism in seed plants. Nature Communications. Springer
Nature. https://doi.org/10.1038/s41467-019-11471-8
chicago: Zhang, Yuzhou, G Xiao, X Wang, Xixi Zhang, and Jiří Friml. “Evolution of
Fast Root Gravitropism in Seed Plants.” Nature Communications. Springer
Nature, 2019. https://doi.org/10.1038/s41467-019-11471-8.
ieee: Y. Zhang, G. Xiao, X. Wang, X. Zhang, and J. Friml, “Evolution of fast root
gravitropism in seed plants,” Nature Communications, vol. 10. Springer
Nature, 2019.
ista: Zhang Y, Xiao G, Wang X, Zhang X, Friml J. 2019. Evolution of fast root gravitropism
in seed plants. Nature Communications. 10, 3480.
mla: Zhang, Yuzhou, et al. “Evolution of Fast Root Gravitropism in Seed Plants.”
Nature Communications, vol. 10, 3480, Springer Nature, 2019, doi:10.1038/s41467-019-11471-8.
short: Y. Zhang, G. Xiao, X. Wang, X. Zhang, J. Friml, Nature Communications 10
(2019).
date_created: 2019-08-09T08:46:26Z
date_published: 2019-08-02T00:00:00Z
date_updated: 2023-08-29T07:02:44Z
day: '02'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41467-019-11471-8
ec_funded: 1
external_id:
isi:
- '000478576500012'
pmid:
- '31375675'
file:
- access_level: open_access
checksum: d2c654fdb97f33078f606fe0c298bf6e
content_type: application/pdf
creator: dernst
date_created: 2019-08-12T07:09:20Z
date_updated: 2020-07-14T12:47:40Z
file_id: '6798'
file_name: 2019_NatureComm_Zhang.pdf
file_size: 6406141
relation: main_file
file_date_updated: 2020-07-14T12:47:40Z
has_accepted_license: '1'
intvolume: ' 10'
isi: 1
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
publication: Nature Communications
publication_identifier:
issn:
- 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/when-plant-roots-learned-to-follow-gravity/
scopus_import: '1'
status: public
title: Evolution of fast root gravitropism in seed plants
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 10
year: '2019'
...
---
_id: '6366'
abstract:
- lang: eng
text: Plants have a remarkable capacity to adjust their growth and development to
elevated ambient temperatures. Increased elongation growth of roots, hypocotyls
and petioles in warm temperatures are hallmarks of seedling thermomorphogenesis.
In the last decade, significant progress has been made to identify the molecular
signaling components regulating these growth responses. Increased ambient temperature
utilizes diverse components of the light sensing and signal transduction network
to trigger growth adjustments. However, it remains unknown whether temperature
sensing and responses are universal processes that occur uniformly in all plant
organs. Alternatively, temperature sensing may be confined to specific tissues
or organs, which would require a systemic signal that mediates responses in distal
parts of the plant. Here we show that Arabidopsis (Arabidopsis thaliana) seedlings
show organ-specific transcriptome responses to elevated temperatures, and that
thermomorphogenesis involves both autonomous and organ-interdependent temperature
sensing and signaling. Seedling roots can sense and respond to temperature in
a shoot-independent manner, whereas shoot temperature responses require both local
and systemic processes. The induction of cell elongation in hypocotyls requires
temperature sensing in cotyledons, followed by generation of a mobile auxin signal.
Subsequently, auxin travels to the hypocotyl where it triggers local brassinosteroid-induced
cell elongation in seedling stems, which depends upon a distinct, permissive temperature
sensor in the hypocotyl.
article_processing_charge: No
article_type: original
author:
- first_name: Julia
full_name: Bellstaedt, Julia
last_name: Bellstaedt
- first_name: Jana
full_name: Trenner, Jana
last_name: Trenner
- first_name: Rebecca
full_name: Lippmann, Rebecca
last_name: Lippmann
- first_name: Yvonne
full_name: Poeschl, Yvonne
last_name: Poeschl
- first_name: Xixi
full_name: Zhang, Xixi
id: 61A66458-47E9-11EA-85BA-8AEAAF14E49A
last_name: Zhang
orcid: 0000-0001-7048-4627
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Marcel
full_name: Quint, Marcel
last_name: Quint
- first_name: Carolin
full_name: Delker, Carolin
last_name: Delker
citation:
ama: Bellstaedt J, Trenner J, Lippmann R, et al. A mobile auxin signal connects
temperature sensing in cotyledons with growth responses in hypocotyls. Plant
Physiology. 2019;180(2):757-766. doi:10.1104/pp.18.01377
apa: Bellstaedt, J., Trenner, J., Lippmann, R., Poeschl, Y., Zhang, X., Friml, J.,
… Delker, C. (2019). A mobile auxin signal connects temperature sensing in cotyledons
with growth responses in hypocotyls. Plant Physiology. ASPB. https://doi.org/10.1104/pp.18.01377
chicago: Bellstaedt, Julia, Jana Trenner, Rebecca Lippmann, Yvonne Poeschl, Xixi
Zhang, Jiří Friml, Marcel Quint, and Carolin Delker. “A Mobile Auxin Signal Connects
Temperature Sensing in Cotyledons with Growth Responses in Hypocotyls.” Plant
Physiology. ASPB, 2019. https://doi.org/10.1104/pp.18.01377.
ieee: J. Bellstaedt et al., “A mobile auxin signal connects temperature sensing
in cotyledons with growth responses in hypocotyls,” Plant Physiology, vol.
180, no. 2. ASPB, pp. 757–766, 2019.
ista: Bellstaedt J, Trenner J, Lippmann R, Poeschl Y, Zhang X, Friml J, Quint M,
Delker C. 2019. A mobile auxin signal connects temperature sensing in cotyledons
with growth responses in hypocotyls. Plant Physiology. 180(2), 757–766.
mla: Bellstaedt, Julia, et al. “A Mobile Auxin Signal Connects Temperature Sensing
in Cotyledons with Growth Responses in Hypocotyls.” Plant Physiology, vol.
180, no. 2, ASPB, 2019, pp. 757–66, doi:10.1104/pp.18.01377.
short: J. Bellstaedt, J. Trenner, R. Lippmann, Y. Poeschl, X. Zhang, J. Friml, M.
Quint, C. Delker, Plant Physiology 180 (2019) 757–766.
date_created: 2019-04-30T15:24:22Z
date_published: 2019-06-01T00:00:00Z
date_updated: 2023-09-05T12:25:19Z
day: '01'
department:
- _id: JiFr
doi: 10.1104/pp.18.01377
external_id:
isi:
- '000470086100019'
pmid:
- '31000634'
intvolume: ' 180'
isi: 1
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: www.doi.org/10.1104/pp.18.01377
month: '06'
oa: 1
oa_version: Published Version
page: 757-766
pmid: 1
publication: Plant Physiology
publication_identifier:
eissn:
- 1532-2548
issn:
- 0032-0889
publication_status: published
publisher: ASPB
quality_controlled: '1'
scopus_import: '1'
status: public
title: A mobile auxin signal connects temperature sensing in cotyledons with growth
responses in hypocotyls
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 180
year: '2019'
...
---
_id: '6259'
abstract:
- lang: eng
text: The plant hormone auxin has crucial roles in almost all aspects of plant growth
and development. Concentrations of auxin vary across different tissues, mediating
distinct developmental outcomes and contributing to the functional diversity of
auxin. However, the mechanisms that underlie these activities are poorly understood.
Here we identify an auxin signalling mechanism, which acts in parallel to the
canonical auxin pathway based on the transport inhibitor response1 (TIR1) and
other auxin receptor F-box (AFB) family proteins (TIR1/AFB receptors)1,2, that
translates levels of cellular auxin to mediate differential growth during apical-hook
development. This signalling mechanism operates at the concave side of the apical
hook, and involves auxin-mediated C-terminal cleavage of transmembrane kinase
1 (TMK1). The cytosolic and nucleus-translocated C terminus of TMK1 specifically
interacts with and phosphorylates two non-canonical transcriptional repressors
of the auxin or indole-3-acetic acid (Aux/IAA) family (IAA32 and IAA34), thereby
regulating ARF transcription factors. In contrast to the degradation of Aux/IAA
transcriptional repressors in the canonical pathway, the newly identified mechanism
stabilizes the non-canonical IAA32 and IAA34 transcriptional repressors to regulate
gene expression and ultimately inhibit growth. The auxin–TMK1 signalling pathway
originates at the cell surface, is triggered by high levels of auxin and shares
a partially overlapping set of transcription factors with the TIR1/AFB signalling
pathway. This allows distinct interpretations of different concentrations of cellular
auxin, and thus enables this versatile signalling molecule to mediate complex
developmental outcomes.
article_processing_charge: No
article_type: original
author:
- first_name: Min
full_name: Cao, Min
last_name: Cao
- first_name: Rong
full_name: Chen, Rong
last_name: Chen
- first_name: Pan
full_name: Li, Pan
last_name: Li
- first_name: Yongqiang
full_name: Yu, Yongqiang
last_name: Yu
- first_name: Rui
full_name: Zheng, Rui
last_name: Zheng
- first_name: Danfeng
full_name: Ge, Danfeng
last_name: Ge
- first_name: Wei
full_name: Zheng, Wei
last_name: Zheng
- first_name: Xuhui
full_name: Wang, Xuhui
last_name: Wang
- first_name: Yangtao
full_name: Gu, Yangtao
last_name: Gu
- first_name: Zuzana
full_name: Gelová, Zuzana
id: 0AE74790-0E0B-11E9-ABC7-1ACFE5697425
last_name: Gelová
orcid: 0000-0003-4783-1752
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Heng
full_name: Zhang, Heng
last_name: Zhang
- first_name: Renyi
full_name: Liu, Renyi
last_name: Liu
- first_name: Jun
full_name: He, Jun
last_name: He
- first_name: Tongda
full_name: Xu, Tongda
last_name: Xu
citation:
ama: Cao M, Chen R, Li P, et al. TMK1-mediated auxin signalling regulates differential
growth of the apical hook. Nature. 2019;568:240-243. doi:10.1038/s41586-019-1069-7
apa: Cao, M., Chen, R., Li, P., Yu, Y., Zheng, R., Ge, D., … Xu, T. (2019). TMK1-mediated
auxin signalling regulates differential growth of the apical hook. Nature.
Springer Nature. https://doi.org/10.1038/s41586-019-1069-7
chicago: Cao, Min, Rong Chen, Pan Li, Yongqiang Yu, Rui Zheng, Danfeng Ge, Wei Zheng,
et al. “TMK1-Mediated Auxin Signalling Regulates Differential Growth of the Apical
Hook.” Nature. Springer Nature, 2019. https://doi.org/10.1038/s41586-019-1069-7.
ieee: M. Cao et al., “TMK1-mediated auxin signalling regulates differential
growth of the apical hook,” Nature, vol. 568. Springer Nature, pp. 240–243,
2019.
ista: Cao M, Chen R, Li P, Yu Y, Zheng R, Ge D, Zheng W, Wang X, Gu Y, Gelová Z,
Friml J, Zhang H, Liu R, He J, Xu T. 2019. TMK1-mediated auxin signalling regulates
differential growth of the apical hook. Nature. 568, 240–243.
mla: Cao, Min, et al. “TMK1-Mediated Auxin Signalling Regulates Differential Growth
of the Apical Hook.” Nature, vol. 568, Springer Nature, 2019, pp. 240–43,
doi:10.1038/s41586-019-1069-7.
short: M. Cao, R. Chen, P. Li, Y. Yu, R. Zheng, D. Ge, W. Zheng, X. Wang, Y. Gu,
Z. Gelová, J. Friml, H. Zhang, R. Liu, J. He, T. Xu, Nature 568 (2019) 240–243.
date_created: 2019-04-09T08:37:05Z
date_published: 2019-04-11T00:00:00Z
date_updated: 2023-09-05T14:58:41Z
day: '11'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41586-019-1069-7
ec_funded: 1
external_id:
isi:
- '000464412700050'
pmid:
- '30944466'
file:
- access_level: open_access
checksum: 6b84ab602a34382cf0340a37a1378c75
content_type: application/pdf
creator: dernst
date_created: 2020-11-13T07:37:41Z
date_updated: 2020-11-13T07:37:41Z
file_id: '8751'
file_name: 2019_Nature _Cao_accepted.pdf
file_size: 4321328
relation: main_file
success: 1
file_date_updated: 2020-11-13T07:37:41Z
has_accepted_license: '1'
intvolume: ' 568'
isi: 1
language:
- iso: eng
month: '04'
oa: 1
oa_version: Submitted Version
page: 240-243
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/newly-discovered-mechanism-of-plant-hormone-auxin-acts-the-opposite-way/
scopus_import: '1'
status: public
title: TMK1-mediated auxin signalling regulates differential growth of the apical
hook
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 568
year: '2019'
...
---
_id: '7106'
abstract:
- lang: eng
text: PIN-FORMED (PIN) transporters mediate directional, intercellular movement
of the phytohormone auxin in land plants. To elucidate the evolutionary origins
of this developmentally crucial mechanism, we analysed the single PIN homologue
of a simple green alga Klebsormidium flaccidum. KfPIN functions as a plasma membrane-localized
auxin exporter in land plants and heterologous models. While its role in algae
remains unclear, PIN-driven auxin export is probably an ancient and conserved
trait within streptophytes.
article_processing_charge: No
article_type: original
author:
- first_name: Roman
full_name: Skokan, Roman
last_name: Skokan
- first_name: Eva
full_name: Medvecká, Eva
last_name: Medvecká
- first_name: Tom
full_name: Viaene, Tom
last_name: Viaene
- first_name: Stanislav
full_name: Vosolsobě, Stanislav
last_name: Vosolsobě
- first_name: Marta
full_name: Zwiewka, Marta
last_name: Zwiewka
- first_name: Karel
full_name: Müller, Karel
last_name: Müller
- first_name: Petr
full_name: Skůpa, Petr
last_name: Skůpa
- first_name: Michal
full_name: Karady, Michal
last_name: Karady
- first_name: Yuzhou
full_name: Zhang, Yuzhou
last_name: Zhang
- first_name: Dorina P.
full_name: Janacek, Dorina P.
last_name: Janacek
- first_name: Ulrich Z.
full_name: Hammes, Ulrich Z.
last_name: Hammes
- first_name: Karin
full_name: Ljung, Karin
last_name: Ljung
- first_name: Tomasz
full_name: Nodzyński, Tomasz
last_name: Nodzyński
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Skokan R, Medvecká E, Viaene T, et al. PIN-driven auxin transport emerged early
in streptophyte evolution. Nature Plants. 2019;5(11):1114-1119. doi:10.1038/s41477-019-0542-5
apa: Skokan, R., Medvecká, E., Viaene, T., Vosolsobě, S., Zwiewka, M., Müller, K.,
… Friml, J. (2019). PIN-driven auxin transport emerged early in streptophyte evolution.
Nature Plants. Springer Nature. https://doi.org/10.1038/s41477-019-0542-5
chicago: Skokan, Roman, Eva Medvecká, Tom Viaene, Stanislav Vosolsobě, Marta Zwiewka,
Karel Müller, Petr Skůpa, et al. “PIN-Driven Auxin Transport Emerged Early in
Streptophyte Evolution.” Nature Plants. Springer Nature, 2019. https://doi.org/10.1038/s41477-019-0542-5.
ieee: R. Skokan et al., “PIN-driven auxin transport emerged early in streptophyte
evolution,” Nature Plants, vol. 5, no. 11. Springer Nature, pp. 1114–1119,
2019.
ista: Skokan R, Medvecká E, Viaene T, Vosolsobě S, Zwiewka M, Müller K, Skůpa P,
Karady M, Zhang Y, Janacek DP, Hammes UZ, Ljung K, Nodzyński T, Petrášek J, Friml
J. 2019. PIN-driven auxin transport emerged early in streptophyte evolution. Nature
Plants. 5(11), 1114–1119.
mla: Skokan, Roman, et al. “PIN-Driven Auxin Transport Emerged Early in Streptophyte
Evolution.” Nature Plants, vol. 5, no. 11, Springer Nature, 2019, pp. 1114–19,
doi:10.1038/s41477-019-0542-5.
short: R. Skokan, E. Medvecká, T. Viaene, S. Vosolsobě, M. Zwiewka, K. Müller, P.
Skůpa, M. Karady, Y. Zhang, D.P. Janacek, U.Z. Hammes, K. Ljung, T. Nodzyński,
J. Petrášek, J. Friml, Nature Plants 5 (2019) 1114–1119.
date_created: 2019-11-25T09:08:04Z
date_published: 2019-11-01T00:00:00Z
date_updated: 2023-09-06T11:09:49Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41477-019-0542-5
ec_funded: 1
external_id:
isi:
- '000496526100010'
pmid:
- '31712756'
file:
- access_level: open_access
checksum: 94e0426856aad9a9bd0135d5436efbf1
content_type: application/pdf
creator: dernst
date_created: 2020-10-14T08:54:49Z
date_updated: 2020-10-14T08:54:49Z
file_id: '8660'
file_name: 2019_NaturePlants_Skokan_accepted.pdf
file_size: 1980851
relation: main_file
success: 1
file_date_updated: 2020-10-14T08:54:49Z
has_accepted_license: '1'
intvolume: ' 5'
isi: 1
issue: '11'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Submitted Version
page: 1114-1119
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Nature Plants
publication_identifier:
issn:
- 2055-0278
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: PIN-driven auxin transport emerged early in streptophyte evolution
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 5
year: '2019'
...
---
_id: '7143'
abstract:
- lang: eng
text: Roots grow downwards parallel to the gravity vector, to anchor a plant in
soil and acquire water and nutrients, using a gravitropic mechanism dependent
on the asymmetric distribution of the phytohormone auxin. Recently, Chang et al.
demonstrate that asymmetric distribution of another phytohormone, cytokinin, directs
root growth towards higher water content.
article_processing_charge: No
article_type: original
author:
- first_name: Scott A
full_name: Sinclair, Scott A
id: 2D99FE6A-F248-11E8-B48F-1D18A9856A87
last_name: Sinclair
orcid: 0000-0002-4566-0593
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Sinclair SA, Friml J. Defying gravity: a plant’s quest for moisture. Cell
Research. 2019;29:965-966. doi:10.1038/s41422-019-0254-4'
apa: 'Sinclair, S. A., & Friml, J. (2019). Defying gravity: a plant’s quest
for moisture. Cell Research. Springer Nature. https://doi.org/10.1038/s41422-019-0254-4'
chicago: 'Sinclair, Scott A, and Jiří Friml. “Defying Gravity: A Plant’s Quest for
Moisture.” Cell Research. Springer Nature, 2019. https://doi.org/10.1038/s41422-019-0254-4.'
ieee: 'S. A. Sinclair and J. Friml, “Defying gravity: a plant’s quest for moisture,”
Cell Research, vol. 29. Springer Nature, pp. 965–966, 2019.'
ista: 'Sinclair SA, Friml J. 2019. Defying gravity: a plant’s quest for moisture.
Cell Research. 29, 965–966.'
mla: 'Sinclair, Scott A., and Jiří Friml. “Defying Gravity: A Plant’s Quest for
Moisture.” Cell Research, vol. 29, Springer Nature, 2019, pp. 965–66, doi:10.1038/s41422-019-0254-4.'
short: S.A. Sinclair, J. Friml, Cell Research 29 (2019) 965–966.
date_created: 2019-12-02T12:30:48Z
date_published: 2019-12-01T00:00:00Z
date_updated: 2023-09-06T11:20:58Z
day: '01'
department:
- _id: JiFr
doi: 10.1038/s41422-019-0254-4
external_id:
isi:
- '000500749600001'
pmid:
- '31745287'
intvolume: ' 29'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1038/s41422-019-0254-4
month: '12'
oa: 1
oa_version: Published Version
page: 965-966
pmid: 1
publication: Cell Research
publication_identifier:
eissn:
- 1748-7838
issn:
- 1001-0602
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Defying gravity: a plant''s quest for moisture'
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 29
year: '2019'
...
---
_id: '7182'
abstract:
- lang: eng
text: During infection pathogens secrete small molecules, termed effectors, to manipulate
and control the interaction with their specific hosts. Both the pathogen and the
plant are under high selective pressure to rapidly adapt and co-evolve in what
is usually referred to as molecular arms race. Components of the host’s immune
system form a network that processes information about molecules with a foreign
origin and damage-associated signals, integrating them with developmental and
abiotic cues to adapt the plant’s responses. Both in the case of nucleotide-binding
leucine-rich repeat receptors and leucine-rich repeat receptor kinases interaction
networks have been extensively characterized. However, little is known on whether
pathogenic effectors form complexes to overcome plant immunity and promote disease.
Ustilago maydis, a biotrophic fungal pathogen that infects maize plants, produces
effectors that target hubs in the immune network of the host cell. Here we assess
the capability of U. maydis effector candidates to interact with each other, which
may play a crucial role during the infection process. Using a systematic yeast-two-hybrid
approach and based on a preliminary pooled screen, we selected 63 putative effectors
for one-on-one matings with a library of nearly 300 effector candidates. We found
that 126 of these effector candidates interacted either with themselves or other
predicted effectors. Although the functional relevance of the observed interactions
remains elusive, we propose that the observed abundance in complex formation between
effectors adds an additional level of complexity to effector research and should
be taken into consideration when studying effector evolution and function. Based
on this fundamental finding, we suggest various scenarios which could evolutionarily
drive the formation and stabilization of an effector interactome.
article_number: '1437'
article_processing_charge: No
article_type: original
author:
- first_name: André
full_name: Alcântara, André
last_name: Alcântara
- first_name: Jason
full_name: Bosch, Jason
last_name: Bosch
- first_name: Fahimeh
full_name: Nazari, Fahimeh
last_name: Nazari
- first_name: Gesa
full_name: Hoffmann, Gesa
last_name: Hoffmann
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Simon
full_name: Uhse, Simon
last_name: Uhse
- first_name: Martin A.
full_name: Darino, Martin A.
last_name: Darino
- first_name: Toluwase
full_name: Olukayode, Toluwase
last_name: Olukayode
- first_name: Daniel
full_name: Reumann, Daniel
last_name: Reumann
- first_name: Laura
full_name: Baggaley, Laura
last_name: Baggaley
- first_name: Armin
full_name: Djamei, Armin
last_name: Djamei
citation:
ama: Alcântara A, Bosch J, Nazari F, et al. Systematic Y2H screening reveals extensive
effector-complex formation. Frontiers in Plant Science. 2019;10(11). doi:10.3389/fpls.2019.01437
apa: Alcântara, A., Bosch, J., Nazari, F., Hoffmann, G., Gallei, M. C., Uhse, S.,
… Djamei, A. (2019). Systematic Y2H screening reveals extensive effector-complex
formation. Frontiers in Plant Science. Frontiers. https://doi.org/10.3389/fpls.2019.01437
chicago: Alcântara, André, Jason Bosch, Fahimeh Nazari, Gesa Hoffmann, Michelle
C Gallei, Simon Uhse, Martin A. Darino, et al. “Systematic Y2H Screening Reveals
Extensive Effector-Complex Formation.” Frontiers in Plant Science. Frontiers,
2019. https://doi.org/10.3389/fpls.2019.01437.
ieee: A. Alcântara et al., “Systematic Y2H screening reveals extensive effector-complex
formation,” Frontiers in Plant Science, vol. 10, no. 11. Frontiers, 2019.
ista: Alcântara A, Bosch J, Nazari F, Hoffmann G, Gallei MC, Uhse S, Darino MA,
Olukayode T, Reumann D, Baggaley L, Djamei A. 2019. Systematic Y2H screening reveals
extensive effector-complex formation. Frontiers in Plant Science. 10(11), 1437.
mla: Alcântara, André, et al. “Systematic Y2H Screening Reveals Extensive Effector-Complex
Formation.” Frontiers in Plant Science, vol. 10, no. 11, 1437, Frontiers,
2019, doi:10.3389/fpls.2019.01437.
short: A. Alcântara, J. Bosch, F. Nazari, G. Hoffmann, M.C. Gallei, S. Uhse, M.A.
Darino, T. Olukayode, D. Reumann, L. Baggaley, A. Djamei, Frontiers in Plant Science
10 (2019).
date_created: 2019-12-15T23:00:43Z
date_published: 2019-11-14T00:00:00Z
date_updated: 2023-09-06T14:33:46Z
day: '14'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.3389/fpls.2019.01437
external_id:
isi:
- '000499821700001'
pmid:
- '31803201'
file:
- access_level: open_access
checksum: 995aa838aec2064d93550de82b40bbd1
content_type: application/pdf
creator: dernst
date_created: 2019-12-16T07:58:43Z
date_updated: 2020-07-14T12:47:52Z
file_id: '7185'
file_name: 2019_FrontiersPlant_Alcantara.pdf
file_size: 1532505
relation: main_file
file_date_updated: 2020-07-14T12:47:52Z
has_accepted_license: '1'
intvolume: ' 10'
isi: 1
issue: '11'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
publication: Frontiers in Plant Science
publication_identifier:
eissn:
- 1664462X
publication_status: published
publisher: Frontiers
quality_controlled: '1'
scopus_import: '1'
status: public
title: Systematic Y2H screening reveals extensive effector-complex formation
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 10
year: '2019'
...
---
_id: '6377'
abstract:
- lang: eng
text: Clathrin-mediated endocytosis (CME) is a highly conserved and essential cellular
process in eukaryotic cells, but its dynamic and vital nature makes it challenging
to study using classical genetics tools. In contrast, although small molecules
can acutely and reversibly perturb CME, the few chemical CME inhibitors that have
been applied to plants are either ineffective or show undesirable side effects.
Here, we identify the previously described endosidin9 (ES9) as an inhibitor of
clathrin heavy chain (CHC) function in both Arabidopsis and human cells through
affinity-based target isolation, in vitro binding studies and X-ray crystallography.
Moreover, we present a chemically improved ES9 analog, ES9-17, which lacks the
undesirable side effects of ES9 while retaining the ability to target CHC. ES9
and ES9-17 have expanded the chemical toolbox used to probe CHC function, and
present chemical scaffolds for further design of more specific and potent CHC
inhibitors across different systems.
article_processing_charge: No
article_type: original
author:
- first_name: Wim
full_name: Dejonghe, Wim
last_name: Dejonghe
- first_name: Isha
full_name: Sharma, Isha
last_name: Sharma
- first_name: Bram
full_name: Denoo, Bram
last_name: Denoo
- first_name: Steven
full_name: De Munck, Steven
last_name: De Munck
- first_name: Qing
full_name: Lu, Qing
last_name: Lu
- first_name: Kiril
full_name: Mishev, Kiril
last_name: Mishev
- first_name: Haydar
full_name: Bulut, Haydar
last_name: Bulut
- first_name: Evelien
full_name: Mylle, Evelien
last_name: Mylle
- first_name: Riet
full_name: De Rycke, Riet
last_name: De Rycke
- first_name: Mina K
full_name: Vasileva, Mina K
id: 3407EB18-F248-11E8-B48F-1D18A9856A87
last_name: Vasileva
- first_name: Daniel V.
full_name: Savatin, Daniel V.
last_name: Savatin
- first_name: Wim
full_name: Nerinckx, Wim
last_name: Nerinckx
- first_name: An
full_name: Staes, An
last_name: Staes
- first_name: Andrzej
full_name: Drozdzecki, Andrzej
last_name: Drozdzecki
- first_name: Dominique
full_name: Audenaert, Dominique
last_name: Audenaert
- first_name: Klaas
full_name: Yperman, Klaas
last_name: Yperman
- first_name: Annemieke
full_name: Madder, Annemieke
last_name: Madder
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Daniël
full_name: Van Damme, Daniël
last_name: Van Damme
- first_name: Kris
full_name: Gevaert, Kris
last_name: Gevaert
- first_name: Volker
full_name: Haucke, Volker
last_name: Haucke
- first_name: Savvas N.
full_name: Savvides, Savvas N.
last_name: Savvides
- first_name: Johan
full_name: Winne, Johan
last_name: Winne
- first_name: Eugenia
full_name: Russinova, Eugenia
last_name: Russinova
citation:
ama: Dejonghe W, Sharma I, Denoo B, et al. Disruption of endocytosis through chemical
inhibition of clathrin heavy chain function. Nature Chemical Biology. 2019;15(6):641–649.
doi:10.1038/s41589-019-0262-1
apa: Dejonghe, W., Sharma, I., Denoo, B., De Munck, S., Lu, Q., Mishev, K., … Russinova,
E. (2019). Disruption of endocytosis through chemical inhibition of clathrin heavy
chain function. Nature Chemical Biology. Springer Nature. https://doi.org/10.1038/s41589-019-0262-1
chicago: Dejonghe, Wim, Isha Sharma, Bram Denoo, Steven De Munck, Qing Lu, Kiril
Mishev, Haydar Bulut, et al. “Disruption of Endocytosis through Chemical Inhibition
of Clathrin Heavy Chain Function.” Nature Chemical Biology. Springer Nature,
2019. https://doi.org/10.1038/s41589-019-0262-1.
ieee: W. Dejonghe et al., “Disruption of endocytosis through chemical inhibition
of clathrin heavy chain function,” Nature Chemical Biology, vol. 15, no.
6. Springer Nature, pp. 641–649, 2019.
ista: Dejonghe W, Sharma I, Denoo B, De Munck S, Lu Q, Mishev K, Bulut H, Mylle
E, De Rycke R, Vasileva MK, Savatin DV, Nerinckx W, Staes A, Drozdzecki A, Audenaert
D, Yperman K, Madder A, Friml J, Van Damme D, Gevaert K, Haucke V, Savvides SN,
Winne J, Russinova E. 2019. Disruption of endocytosis through chemical inhibition
of clathrin heavy chain function. Nature Chemical Biology. 15(6), 641–649.
mla: Dejonghe, Wim, et al. “Disruption of Endocytosis through Chemical Inhibition
of Clathrin Heavy Chain Function.” Nature Chemical Biology, vol. 15, no.
6, Springer Nature, 2019, pp. 641–649, doi:10.1038/s41589-019-0262-1.
short: W. Dejonghe, I. Sharma, B. Denoo, S. De Munck, Q. Lu, K. Mishev, H. Bulut,
E. Mylle, R. De Rycke, M.K. Vasileva, D.V. Savatin, W. Nerinckx, A. Staes, A.
Drozdzecki, D. Audenaert, K. Yperman, A. Madder, J. Friml, D. Van Damme, K. Gevaert,
V. Haucke, S.N. Savvides, J. Winne, E. Russinova, Nature Chemical Biology 15 (2019)
641–649.
date_created: 2019-05-05T21:59:11Z
date_published: 2019-06-01T00:00:00Z
date_updated: 2023-09-07T12:54:35Z
day: '01'
department:
- _id: JiFr
doi: 10.1038/s41589-019-0262-1
external_id:
isi:
- '000468195600018'
intvolume: ' 15'
isi: 1
issue: '6'
language:
- iso: eng
month: '06'
oa_version: None
page: 641–649
publication: Nature Chemical Biology
publication_identifier:
eissn:
- '15524469'
issn:
- '15524450'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
record:
- id: '7172'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Disruption of endocytosis through chemical inhibition of clathrin heavy chain
function
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 15
year: '2019'
...
---
_id: '7172'
abstract:
- lang: eng
text: "The development and growth of Arabidopsis thaliana is regulated by a combination
of genetic programing and also by the environmental influences. An important role
in these processes play the phytohormones and among them, auxin is crucial as
it controls many important functions. It is transported through the whole plant
body by creating local and temporal concentration maxima and minima, which have
an impact on the cell status, tissue and organ identity. Auxin has the property
to undergo a directional and finely regulated cell-to-cell transport, which is
enabled by the transport proteins, localized on the plasma membrane. An important
role in this process have the PIN auxin efflux proteins, which have an asymmetric/polar
subcellular localization and determine the directionality of the auxin transport.
During the last years, there were significant advances in understanding how the
trafficking molecular machineries function, including studies on molecular interactions,
function, subcellular localization and intracellular distribution. However, there
is still a lack of detailed characterization on the steps of endocytosis, exocytosis,
endocytic recycling and degradation. Due to this fact, I focused on the identification
of novel trafficking factors and better characterization of the intracellular
trafficking pathways. My PhD thesis consists of an introductory chapter, three
experimental chapters, a chapter containing general discussion, conclusions and
perspectives and also an appendix chapter with published collaborative papers.\r\nThe
first chapter is separated in two different parts: I start by a general introduction
to auxin biology and then I introduce the trafficking pathways in the model plant
Arabidopsis thaliana. Then, I explain also the phosphorylation-signals for polar
targeting and also the roles of the phytohormone strigolactone.\r\nThe second
chapter includes the characterization of bar1/sacsin mutant, which was identified
in a forward genetic screen for novel trafficking components in Arabidopsis thaliana,
where by the implementation of an EMS-treated pPIN1::PIN1-GFP marker line and
by using the established inhibitor of ARF-GEFs, Brefeldin A (BFA) as a tool to
study trafficking processes, we identified a novel factor, which is mediating
the adaptation of the plant cell to ARF-GEF inhibition. The mutation is in a previously
uncharacterized gene, encoding a very big protein that we, based on its homologies,
called SACSIN with domains suggesting roles as a molecular chaperon or as a component
of the ubiquitin-proteasome system. Our physiology and imaging studies revealed
that SACSIN is a crucial plant cell component of the adaptation to the ARF-GEF
inhibition.\r\nThe third chapter includes six subchapters, where I focus on the
role of the phytohormone strigolactone, which interferes with auxin feedback on
PIN internalization. Strigolactone moderates the polar auxin transport by increasing
the internalization of the PIN auxin efflux carriers, which reduces the canalization
related growth responses. In addition, I also studied the role of phosphorylation
in the strigolactone regulation of auxin feedback on PIN internalization. In this
chapter I also present my results on the MAX2-dependence of strigolactone-mediated
root growth inhibition and I also share my results on the auxin metabolomics profiling
after application of GR24.\r\nIn the fourth chapter I studied the effect of two
small molecules ES-9 and ES9-17, which were identified from a collection of small
molecules with the property to impair the clathrin-mediated endocytosis.\r\nIn
the fifth chapter, I discuss all my observations and experimental findings and
suggest alternative hypothesis to interpret my results.\r\nIn the appendix there
are three collaborative published projects. In the first, I participated in the
characterization of the role of ES9 as a small molecule, which is inhibitor of
clathrin- mediated endocytosis in different model organisms. In the second paper,
I contributed to the characterization of another small molecule ES9-17, which
is a non-protonophoric analog of ES9 and also impairs the clathrin-mediated endocytosis
not only in plant cells, but also in mammalian HeLa cells. Last but not least,
I also attach another paper, where I tried to establish the grafting method as
a technique in our lab to study canalization related processes."
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Mina K
full_name: Vasileva, Mina K
id: 3407EB18-F248-11E8-B48F-1D18A9856A87
last_name: Vasileva
citation:
ama: Vasileva MK. Molecular mechanisms of endomembrane trafficking in Arabidopsis
thaliana. 2019. doi:10.15479/AT:ISTA:7172
apa: Vasileva, M. K. (2019). Molecular mechanisms of endomembrane trafficking
in Arabidopsis thaliana. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:7172
chicago: Vasileva, Mina K. “Molecular Mechanisms of Endomembrane Trafficking in
Arabidopsis Thaliana.” Institute of Science and Technology Austria, 2019. https://doi.org/10.15479/AT:ISTA:7172.
ieee: M. K. Vasileva, “Molecular mechanisms of endomembrane trafficking in Arabidopsis
thaliana,” Institute of Science and Technology Austria, 2019.
ista: Vasileva MK. 2019. Molecular mechanisms of endomembrane trafficking in Arabidopsis
thaliana. Institute of Science and Technology Austria.
mla: Vasileva, Mina K. Molecular Mechanisms of Endomembrane Trafficking in Arabidopsis
Thaliana. Institute of Science and Technology Austria, 2019, doi:10.15479/AT:ISTA:7172.
short: M.K. Vasileva, Molecular Mechanisms of Endomembrane Trafficking in Arabidopsis
Thaliana, Institute of Science and Technology Austria, 2019.
date_created: 2019-12-11T21:24:39Z
date_published: 2019-12-12T00:00:00Z
date_updated: 2023-09-19T10:39:33Z
day: '12'
ddc:
- '570'
degree_awarded: PhD
department:
- _id: JiFr
doi: 10.15479/AT:ISTA:7172
file:
- access_level: closed
checksum: ef981c1a3b1d9da0edcbedcff4970d37
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: mvasilev
date_created: 2019-12-12T09:32:36Z
date_updated: 2020-07-14T12:47:51Z
file_id: '7175'
file_name: Thesis_Mina_final_upload_7.docx
file_size: 20454014
relation: source_file
- access_level: open_access
checksum: 3882c4585e46c9cfb486e4225cad54ab
content_type: application/pdf
creator: mvasilev
date_created: 2019-12-12T09:33:10Z
date_updated: 2020-07-14T12:47:51Z
file_id: '7176'
file_name: Thesis_Mina_final_upload_7.pdf
file_size: 11565025
relation: main_file
file_date_updated: 2020-07-14T12:47:51Z
has_accepted_license: '1'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
page: '192'
publication_identifier:
eissn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '1346'
relation: part_of_dissertation
status: public
- id: '6377'
relation: part_of_dissertation
status: public
- id: '449'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
title: Molecular mechanisms of endomembrane trafficking in Arabidopsis thaliana
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2019'
...
---
_id: '6999'
abstract:
- lang: eng
text: Plasmodesmata (PD) are plant-specific membrane-lined channels that create
cytoplasmic and membrane continuities between adjacent cells, thereby facilitating
cell–cell communication and virus movement. Plant cells have evolved diverse mechanisms
to regulate PD plasticity in response to numerous environmental stimuli. In particular,
during defense against plant pathogens, the defense hormone, salicylic acid (SA),
plays a crucial role in the regulation of PD permeability in a callose-dependent
manner. Here, we uncover a mechanism by which plants restrict the spreading of
virus and PD cargoes using SA signaling by increasing lipid order and closure
of PD. We showed that exogenous SA application triggered the compartmentalization
of lipid raft nanodomains through a modulation of the lipid raft-regulatory protein,
Remorin (REM). Genetic studies, superresolution imaging, and transmission electron
microscopy observation together demonstrated that Arabidopsis REM1.2 and REM1.3
are crucial for plasma membrane nanodomain assembly to control PD aperture and
functionality. In addition, we also found that a 14-3-3 epsilon protein modulates
REM clustering and membrane nanodomain compartmentalization through its direct
interaction with REM proteins. This study unveils a molecular mechanism by which
the key plant defense hormone, SA, triggers membrane lipid nanodomain reorganization,
thereby regulating PD closure to impede virus spreading.
article_processing_charge: No
article_type: original
author:
- first_name: D
full_name: Huang, D
last_name: Huang
- first_name: Y
full_name: Sun, Y
last_name: Sun
- first_name: Z
full_name: Ma, Z
last_name: Ma
- first_name: M
full_name: Ke, M
last_name: Ke
- first_name: Y
full_name: Cui, Y
last_name: Cui
- first_name: Z
full_name: Chen, Z
last_name: Chen
- first_name: C
full_name: Chen, C
last_name: Chen
- first_name: C
full_name: Ji, C
last_name: Ji
- first_name: TM
full_name: Tran, TM
last_name: Tran
- first_name: L
full_name: Yang, L
last_name: Yang
- first_name: SM
full_name: Lam, SM
last_name: Lam
- first_name: Y
full_name: Han, Y
last_name: Han
- first_name: G
full_name: Shu, G
last_name: Shu
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Y
full_name: Miao, Y
last_name: Miao
- first_name: L
full_name: Jiang, L
last_name: Jiang
- first_name: X
full_name: Chen, X
last_name: Chen
citation:
ama: Huang D, Sun Y, Ma Z, et al. Salicylic acid-mediated plasmodesmal closure via
Remorin-dependent lipid organization. Proceedings of the National Academy of
Sciences of the United States of America. 2019;116(42):21274-21284. doi:10.1073/pnas.1911892116
apa: Huang, D., Sun, Y., Ma, Z., Ke, M., Cui, Y., Chen, Z., … Chen, X. (2019). Salicylic
acid-mediated plasmodesmal closure via Remorin-dependent lipid organization. Proceedings
of the National Academy of Sciences of the United States of America. Proceedings
of the National Academy of Sciences. https://doi.org/10.1073/pnas.1911892116
chicago: Huang, D, Y Sun, Z Ma, M Ke, Y Cui, Z Chen, C Chen, et al. “Salicylic Acid-Mediated
Plasmodesmal Closure via Remorin-Dependent Lipid Organization.” Proceedings
of the National Academy of Sciences of the United States of America. Proceedings
of the National Academy of Sciences, 2019. https://doi.org/10.1073/pnas.1911892116.
ieee: D. Huang et al., “Salicylic acid-mediated plasmodesmal closure via
Remorin-dependent lipid organization,” Proceedings of the National Academy
of Sciences of the United States of America, vol. 116, no. 42. Proceedings
of the National Academy of Sciences, pp. 21274–21284, 2019.
ista: Huang D, Sun Y, Ma Z, Ke M, Cui Y, Chen Z, Chen C, Ji C, Tran T, Yang L, Lam
S, Han Y, Shu G, Friml J, Miao Y, Jiang L, Chen X. 2019. Salicylic acid-mediated
plasmodesmal closure via Remorin-dependent lipid organization. Proceedings of
the National Academy of Sciences of the United States of America. 116(42), 21274–21284.
mla: Huang, D., et al. “Salicylic Acid-Mediated Plasmodesmal Closure via Remorin-Dependent
Lipid Organization.” Proceedings of the National Academy of Sciences of the
United States of America, vol. 116, no. 42, Proceedings of the National Academy
of Sciences, 2019, pp. 21274–84, doi:10.1073/pnas.1911892116.
short: D. Huang, Y. Sun, Z. Ma, M. Ke, Y. Cui, Z. Chen, C. Chen, C. Ji, T. Tran,
L. Yang, S. Lam, Y. Han, G. Shu, J. Friml, Y. Miao, L. Jiang, X. Chen, Proceedings
of the National Academy of Sciences of the United States of America 116 (2019)
21274–21284.
date_created: 2019-11-12T11:42:05Z
date_published: 2019-10-15T00:00:00Z
date_updated: 2023-10-17T12:32:37Z
day: '15'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1073/pnas.1911892116
external_id:
isi:
- '000490183000068'
pmid:
- '31575745'
file:
- access_level: open_access
checksum: 258c666bc6253eab81961f61169eefae
content_type: application/pdf
creator: dernst
date_created: 2019-11-13T08:22:28Z
date_updated: 2020-07-14T12:47:46Z
file_id: '7012'
file_name: 2019_PNAS_Huang.pdf
file_size: 3287466
relation: main_file
file_date_updated: 2020-07-14T12:47:46Z
has_accepted_license: '1'
intvolume: ' 116'
isi: 1
issue: '42'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
page: 21274-21284
pmid: 1
publication: Proceedings of the National Academy of Sciences of the United States
of America
publication_identifier:
eissn:
- 1091-6490
issn:
- 0027-8424
publication_status: published
publisher: Proceedings of the National Academy of Sciences
quality_controlled: '1'
related_material:
link:
- relation: erratum
url: https://doi.org/10.1073/pnas.2004738117
scopus_import: '1'
status: public
title: Salicylic acid-mediated plasmodesmal closure via Remorin-dependent lipid organization
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 116
year: '2019'
...
---
_id: '6269'
abstract:
- lang: eng
text: 'Clathrin-Mediated Endocytosis (CME) is an aspect of cellular trafficking
that is constantly regulated for mediating developmental and physiological responses.
The main aim of my thesis is to decipher the basic mechanisms of CME and post-endocytic
trafficking in the whole multicellular organ systems of Arabidopsis. The first
chapter of my thesis describes the search for new components involved in CME.
Tandem affinity purification was conducted using CLC and its interacting partners
were identified. Amongst the identified proteins were the Auxilin-likes1 and 2
(Axl1/2), putative uncoating factors, for which we made a full functional analysis.
Over-expression of Axl1/2 causes extreme modifications in the dynamics of the
machinery proteins and inhibition of endocytosis altogether. However the loss
of function of the axl1/2 did not present any cellular or physiological phenotype,
meaning Auxilin-likes do not form the major uncoating machinery. The second chapter
of my thesis describes the establishment/utilisation of techniques to capture
the dynamicity and the complexity of CME and post-endocytic trafficking. We have
studied the development of endocytic pits at the PM – specifically, the mode of
membrane remodeling during pit development and the role of actin in it, given
plant cells possess high turgor pressure. Utilizing the improved z-resolution
of TIRF and VAEM techniques, we captured the time-lapse of the endocytic events
at the plasma membrane; and using particle detection software, we quantitatively
analysed all the endocytic trajectories in an unbiased way to obtain the endocytic
rate of the system. This together with the direct analysis of cargo internalisation
from the PM provided an estimate on the endocytic potential of the cell. We also
developed a methodology for ultrastructural analysis of different populations
of Clathrin-Coated Structures (CCSs) in both PM and endomembranes in unroofed
protoplasts. Structural analysis, together with the intensity profile of CCSs
at the PM show that the mode of CCP development at the PM follows ‘Constant curvature
model’; meaning that clathrin polymerisation energy is a major contributing factor
of membrane remodeling. In addition, other analyses clearly show that actin is
not required for membrane remodeling during invagination or any other step of
CCP development, despite the prevalent high turgor pressure. However, actin is
essential in orchestrating the post-endocytic trafficking of CCVs facilitating
the EE formation. We also observed that the uncoating process post-endocytosis
is not immediate; an alternative mechanism of uncoating – Sequential multi-step
process – functions in the cell. Finally we also looked at one of the important
physiological stimuli modulating the process – hormone, auxin. auxin has been
known to influence CME before. We have made a detailed study on the concentration-time
based effect of auxin on the machinery proteins, CCP development, and the specificity
of cargoes endocytosed. To this end, we saw no general effect of auxin on CME
at earlier time points. However, very low concentration of IAA, such as 50nM,
accelerates endocytosis of specifically PIN2 through CME. Such a tight regulatory
control with high specificity to PIN2 could be essential in modulating its polarity. '
acknowledged_ssus:
- _id: Bio
- _id: EM-Fac
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Madhumitha
full_name: Narasimhan, Madhumitha
id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
last_name: Narasimhan
orcid: 0000-0002-8600-0671
citation:
ama: Narasimhan M. Clathrin-Mediated endocytosis, post-endocytic trafficking and
their regulatory controls in plants . 2019. doi:10.15479/at:ista:th1075
apa: Narasimhan, M. (2019). Clathrin-Mediated endocytosis, post-endocytic trafficking
and their regulatory controls in plants . Institute of Science and Technology
Austria. https://doi.org/10.15479/at:ista:th1075
chicago: Narasimhan, Madhumitha. “Clathrin-Mediated Endocytosis, Post-Endocytic
Trafficking and Their Regulatory Controls in Plants .” Institute of Science and
Technology Austria, 2019. https://doi.org/10.15479/at:ista:th1075.
ieee: M. Narasimhan, “Clathrin-Mediated endocytosis, post-endocytic trafficking
and their regulatory controls in plants ,” Institute of Science and Technology
Austria, 2019.
ista: Narasimhan M. 2019. Clathrin-Mediated endocytosis, post-endocytic trafficking
and their regulatory controls in plants . Institute of Science and Technology
Austria.
mla: Narasimhan, Madhumitha. Clathrin-Mediated Endocytosis, Post-Endocytic Trafficking
and Their Regulatory Controls in Plants . Institute of Science and Technology
Austria, 2019, doi:10.15479/at:ista:th1075.
short: M. Narasimhan, Clathrin-Mediated Endocytosis, Post-Endocytic Trafficking
and Their Regulatory Controls in Plants , Institute of Science and Technology
Austria, 2019.
date_created: 2019-04-09T14:37:06Z
date_published: 2019-02-04T00:00:00Z
date_updated: 2023-09-08T11:43:03Z
day: '04'
ddc:
- '575'
degree_awarded: PhD
department:
- _id: JiFr
doi: 10.15479/at:ista:th1075
file:
- access_level: open_access
checksum: c958f27dd752712886e7e2638b847a3c
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:18Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6270'
file_name: Supplementary_movie_1.avi
file_size: 5402078
relation: main_file
- access_level: open_access
checksum: 8786fdc29c62987c0aad3c866a4d3691
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:18Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6271'
file_name: 3.7_supplementary_movie_10.avi
file_size: 5927736
relation: main_file
- access_level: open_access
checksum: 25f784c5159d6f4d966b2f9b371ebaf6
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:18Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6272'
file_name: 3.7_supplementary_movie_9.avi
file_size: 9570210
relation: main_file
- access_level: open_access
checksum: 917069272a7a08d1f38224d5e12765d6
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:18Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6273'
file_name: 3.7_supplementary_movie_8.avi
file_size: 2827360
relation: main_file
- access_level: open_access
checksum: 81e74f5ca0ad70050504f18192236dc0
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:18Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6274'
file_name: 3.7_supplementary_movie_7.avi
file_size: 5771410
relation: main_file
- access_level: open_access
checksum: 47eb37b27a2930252713924307ea8c6f
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:18Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6275'
file_name: 3.7_supplementary_movie_6.avi
file_size: 1113486
relation: main_file
- access_level: open_access
checksum: f68f66721041ce84e331959c9a5779c3
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:18Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6276'
file_name: 3.7_supplementary_movie_5.avi
file_size: 1057232
relation: main_file
- access_level: open_access
checksum: 67c01cefab51b363c5e214fe4cd671f3
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:23Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6277'
file_name: 3.7_supplementary_movie_3.avi
file_size: 127472916
relation: main_file
- access_level: open_access
checksum: e5a397edbee05b8821e2b19b3c1a9260
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:19Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6278'
file_name: 3.7_supplementary_movie_4.avi
file_size: 3181238
relation: main_file
- access_level: open_access
checksum: 32d92b2a9277f956fdb0b42351d07c0b
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:19Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6279'
file_name: 3.7_supplementary_movie_2.avi
file_size: 5970952
relation: main_file
- access_level: open_access
checksum: efe7001f5d9a8c61e631e12d5f324ade
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:21Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6280'
file_name: 3.7_Supplementary_movie_1.avi
file_size: 39835236
relation: main_file
- access_level: open_access
checksum: eeb0a5603c6449c5f34eacd5ff0b3a16
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:21Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6281'
file_name: 2.5_Suppl_Movie_4_AP2A1_TagRFP.avi
file_size: 3696740
relation: main_file
- access_level: open_access
checksum: 8e7c00ef6223bf0e177deb168338af13
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:21Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6282'
file_name: 2.5_Suppl_Movie_3_TPLATE_GFP.avi
file_size: 6741232
relation: main_file
- access_level: open_access
checksum: 3636006a7cb709a7543d6581e359b28d
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:22Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6283'
file_name: 2.5_Suppl_Movie_2_CLC_GFP.avi
file_size: 2445946
relation: main_file
- access_level: open_access
checksum: 39ca5519a6e9a38356e7b3704004fea7
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:22Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6284'
file_name: 2.5_Suppl_Movie_1_CLC_GFPxAxl1_mcherry.avi
file_size: 58594
relation: main_file
- access_level: open_access
checksum: 4fcdaa3a6c645514a3b3205f0f69dc76
content_type: application/pdf
creator: dernst
date_created: 2019-04-09T14:35:33Z
date_updated: 2021-02-11T11:17:15Z
embargo: 2020-02-11
file_id: '6285'
file_name: 2019_Thesis_Narasimhan.pdf
file_size: 10553937
relation: main_file
- access_level: closed
checksum: 268f0b6bad21d5f0d671e5d4b88104a7
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: dernst
date_created: 2019-04-09T14:35:36Z
date_updated: 2020-07-14T12:47:26Z
embargo_to: open_access
file_id: '6286'
file_name: 2019_Thesis_Narasimhan_source.docx
file_size: 135291990
relation: source_file
file_date_updated: 2021-02-11T23:30:15Z
has_accepted_license: '1'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: '138'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '412'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
title: 'Clathrin-Mediated endocytosis, post-endocytic trafficking and their regulatory
controls in plants '
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2019'
...
---
_id: '6351'
abstract:
- lang: eng
text: "A process of restorative patterning in plant roots correctly replaces eliminated
cells to heal local injuries despite the absence of cell migration, which underpins
wound healing in animals. \r\n\r\nPatterning in plants relies on oriented cell
divisions and acquisition of specific cell identities. Plants regularly endure
wounds caused by abiotic or biotic environmental stimuli and have developed extraordinary
abilities to restore their tissues after injuries. Here, we provide insight into
a mechanism of restorative patterning that repairs tissues after wounding. Laser-assisted
elimination of different cells in Arabidopsis root combined with live-imaging
tracking during vertical growth allowed analysis of the regeneration processes
in vivo. Specifically, the cells adjacent to the inner side of the injury re-activated
their stem cell transcriptional programs. They accelerated their progression through
cell cycle, coordinately changed the cell division orientation, and ultimately
acquired de novo the correct cell fates to replace missing cells. These observations
highlight existence of unknown intercellular positional signaling and demonstrate
the capability of specified cells to re-acquire stem cell programs as a crucial
part of the plant-specific mechanism of wound healing."
acknowledged_ssus:
- _id: Bio
article_processing_charge: No
author:
- first_name: Petra
full_name: Marhavá, Petra
id: 44E59624-F248-11E8-B48F-1D18A9856A87
last_name: Marhavá
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
orcid: 0000-0001-8295-2926
- first_name: Saiko
full_name: Yoshida, Saiko
id: 2E46069C-F248-11E8-B48F-1D18A9856A87
last_name: Yoshida
- first_name: Peter
full_name: Marhavy, Peter
id: 3F45B078-F248-11E8-B48F-1D18A9856A87
last_name: Marhavy
orcid: 0000-0001-5227-5741
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Marhavá P, Hörmayer L, Yoshida S, Marhavý P, Benková E, Friml J. Re-activation
of stem cell pathways for pattern restoration in plant wound healing. Cell.
2019;177(4):957-969.e13. doi:10.1016/j.cell.2019.04.015
apa: Marhavá, P., Hörmayer, L., Yoshida, S., Marhavý, P., Benková, E., & Friml,
J. (2019). Re-activation of stem cell pathways for pattern restoration in plant
wound healing. Cell. Elsevier. https://doi.org/10.1016/j.cell.2019.04.015
chicago: Marhavá, Petra, Lukas Hörmayer, Saiko Yoshida, Peter Marhavý, Eva Benková,
and Jiří Friml. “Re-Activation of Stem Cell Pathways for Pattern Restoration in
Plant Wound Healing.” Cell. Elsevier, 2019. https://doi.org/10.1016/j.cell.2019.04.015.
ieee: P. Marhavá, L. Hörmayer, S. Yoshida, P. Marhavý, E. Benková, and J. Friml,
“Re-activation of stem cell pathways for pattern restoration in plant wound healing,”
Cell, vol. 177, no. 4. Elsevier, p. 957–969.e13, 2019.
ista: Marhavá P, Hörmayer L, Yoshida S, Marhavý P, Benková E, Friml J. 2019. Re-activation
of stem cell pathways for pattern restoration in plant wound healing. Cell. 177(4),
957–969.e13.
mla: Marhavá, Petra, et al. “Re-Activation of Stem Cell Pathways for Pattern Restoration
in Plant Wound Healing.” Cell, vol. 177, no. 4, Elsevier, 2019, p. 957–969.e13,
doi:10.1016/j.cell.2019.04.015.
short: P. Marhavá, L. Hörmayer, S. Yoshida, P. Marhavý, E. Benková, J. Friml, Cell
177 (2019) 957–969.e13.
date_created: 2019-04-28T21:59:14Z
date_published: 2019-05-02T00:00:00Z
date_updated: 2024-03-18T23:30:10Z
day: '02'
ddc:
- '570'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1016/j.cell.2019.04.015
ec_funded: 1
external_id:
isi:
- '000466843000015'
pmid:
- '31051107'
file:
- access_level: open_access
checksum: 4ceba04a96a74f5092ec3ce2c579a0c7
content_type: application/pdf
creator: dernst
date_created: 2019-05-13T06:12:45Z
date_updated: 2020-07-14T12:47:28Z
file_id: '6411'
file_name: 2019_Cell_Marhava.pdf
file_size: 10272032
relation: main_file
file_date_updated: 2020-07-14T12:47:28Z
has_accepted_license: '1'
intvolume: ' 177'
isi: 1
issue: '4'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
page: 957-969.e13
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Cell
publication_identifier:
eissn:
- '10974172'
issn:
- '00928674'
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/specialized-plant-cells-regain-stem-cell-features-to-heal-wounds/
record:
- id: '9992'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Re-activation of stem cell pathways for pattern restoration in plant wound
healing
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 177
year: '2019'
...
---
_id: '6943'
abstract:
- lang: eng
text: Plants as sessile organisms are constantly under attack by herbivores, rough
environmental situations, or mechanical pressure. These challenges often lead
to the induction of wounds or destruction of already specified and developed tissues.
Additionally, wounding makes plants vulnerable to invasion by pathogens, which
is why wound signalling often triggers specific defence responses. To stay competitive
or, eventually, survive under these circumstances, plants need to regenerate efficiently,
which in rigid, tissue migration-incompatible plant tissues requires post-embryonic
patterning and organogenesis. Now, several studies used laser-assisted single
cell ablation in the Arabidopsis root tip as a minimal wounding proxy. Here, we
discuss their findings and put them into context of a broader spectrum of wound
signalling, pathogen responses and tissue as well as organ regeneration.
article_processing_charge: No
article_type: original
author:
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
orcid: 0000-0001-8295-2926
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Hörmayer L, Friml J. Targeted cell ablation-based insights into wound healing
and restorative patterning. Current Opinion in Plant Biology. 2019;52:124-130.
doi:10.1016/j.pbi.2019.08.006
apa: Hörmayer, L., & Friml, J. (2019). Targeted cell ablation-based insights
into wound healing and restorative patterning. Current Opinion in Plant Biology.
Elsevier. https://doi.org/10.1016/j.pbi.2019.08.006
chicago: Hörmayer, Lukas, and Jiří Friml. “Targeted Cell Ablation-Based Insights
into Wound Healing and Restorative Patterning.” Current Opinion in Plant Biology.
Elsevier, 2019. https://doi.org/10.1016/j.pbi.2019.08.006.
ieee: L. Hörmayer and J. Friml, “Targeted cell ablation-based insights into wound
healing and restorative patterning,” Current Opinion in Plant Biology,
vol. 52. Elsevier, pp. 124–130, 2019.
ista: Hörmayer L, Friml J. 2019. Targeted cell ablation-based insights into wound
healing and restorative patterning. Current Opinion in Plant Biology. 52, 124–130.
mla: Hörmayer, Lukas, and Jiří Friml. “Targeted Cell Ablation-Based Insights into
Wound Healing and Restorative Patterning.” Current Opinion in Plant Biology,
vol. 52, Elsevier, 2019, pp. 124–30, doi:10.1016/j.pbi.2019.08.006.
short: L. Hörmayer, J. Friml, Current Opinion in Plant Biology 52 (2019) 124–130.
date_created: 2019-10-14T07:00:24Z
date_published: 2019-12-01T00:00:00Z
date_updated: 2024-03-18T23:30:10Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.pbi.2019.08.006
ec_funded: 1
external_id:
isi:
- '000502890600017'
pmid:
- '31585333'
file:
- access_level: open_access
checksum: d6fd68a6e965f1efe3f0bf2d2070a616
content_type: application/pdf
creator: dernst
date_created: 2019-10-14T14:48:21Z
date_updated: 2020-07-14T12:47:45Z
file_id: '6946'
file_name: 2019_CurrentOpinionPlant_Hoermayer.pdf
file_size: 1659288
relation: main_file
file_date_updated: 2020-07-14T12:47:45Z
has_accepted_license: '1'
intvolume: ' 52'
isi: 1
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
page: 124-130
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Current Opinion in Plant Biology
publication_identifier:
issn:
- 1369-5266
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
record:
- id: '9992'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Targeted cell ablation-based insights into wound healing and restorative patterning
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 52
year: '2019'
...
---
_id: '6260'
abstract:
- lang: eng
text: Polar auxin transport plays a pivotal role in plant growth and development.
PIN auxin efflux carriers regulate directional auxin movement by establishing
local auxin maxima, minima, and gradients that drive multiple developmental processes
and responses to environmental signals. Auxin has been proposed to modulate its
own transport by regulating subcellular PIN trafficking via processes such as
clathrin-mediated PIN endocytosis and constitutive recycling. Here, we further
investigated the mechanisms by which auxin affects PIN trafficking by screening
auxin analogs and identified pinstatic acid (PISA) as a positive modulator of
polar auxin transport in Arabidopsis thaliana. PISA had an auxin-like effect on
hypocotyl elongation and adventitious root formation via positive regulation of
auxin transport. PISA did not activate SCFTIR1/AFB signaling and yet induced PIN
accumulation at the cell surface by inhibiting PIN internalization from the plasma
membrane. This work demonstrates PISA to be a promising chemical tool to dissect
the regulatory mechanisms behind subcellular PIN trafficking and auxin transport.
acknowledgement: "We thank Dr. H. Fukaki (University of Kobe), Dr. R. Offringa (Leiden
University), Dr. Jianwei Pan (Zhejiang Normal University), and Dr. M. Estelle (University
of California at San Diego) for providing mutants and transgenic line seeds.\r\nThis
work was supported by the Ministry of Education, Culture, Sports, Science, and Technology
(Grant-in-Aid for Scientific Research no. JP25114518 to K.H.), the Biotechnology
and Biological Sciences Research Council (award no. BB/L009366/1 to R.N. and S.K.),
and the European Union’s Horizon2020 program (European Research Council grant agreement
no. 742985 to J.F.)."
article_processing_charge: No
article_type: original
author:
- first_name: A
full_name: Oochi, A
last_name: Oochi
- first_name: Jakub
full_name: Hajny, Jakub
id: 4800CC20-F248-11E8-B48F-1D18A9856A87
last_name: Hajny
orcid: 0000-0003-2140-7195
- first_name: K
full_name: Fukui, K
last_name: Fukui
- first_name: Y
full_name: Nakao, Y
last_name: Nakao
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: M
full_name: Quareshy, M
last_name: Quareshy
- first_name: K
full_name: Takahashi, K
last_name: Takahashi
- first_name: T
full_name: Kinoshita, T
last_name: Kinoshita
- first_name: SR
full_name: Harborough, SR
last_name: Harborough
- first_name: S
full_name: Kepinski, S
last_name: Kepinski
- first_name: H
full_name: Kasahara, H
last_name: Kasahara
- first_name: RM
full_name: Napier, RM
last_name: Napier
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: KI
full_name: Hayashi, KI
last_name: Hayashi
citation:
ama: Oochi A, Hajny J, Fukui K, et al. Pinstatic acid promotes auxin transport by
inhibiting PIN internalization. Plant Physiology. 2019;180(2):1152-1165.
doi:10.1104/pp.19.00201
apa: Oochi, A., Hajny, J., Fukui, K., Nakao, Y., Gallei, M. C., Quareshy, M., …
Hayashi, K. (2019). Pinstatic acid promotes auxin transport by inhibiting PIN
internalization. Plant Physiology. ASPB. https://doi.org/10.1104/pp.19.00201
chicago: Oochi, A, Jakub Hajny, K Fukui, Y Nakao, Michelle C Gallei, M Quareshy,
K Takahashi, et al. “Pinstatic Acid Promotes Auxin Transport by Inhibiting PIN
Internalization.” Plant Physiology. ASPB, 2019. https://doi.org/10.1104/pp.19.00201.
ieee: A. Oochi et al., “Pinstatic acid promotes auxin transport by inhibiting
PIN internalization,” Plant Physiology, vol. 180, no. 2. ASPB, pp. 1152–1165,
2019.
ista: Oochi A, Hajny J, Fukui K, Nakao Y, Gallei MC, Quareshy M, Takahashi K, Kinoshita
T, Harborough S, Kepinski S, Kasahara H, Napier R, Friml J, Hayashi K. 2019. Pinstatic
acid promotes auxin transport by inhibiting PIN internalization. Plant Physiology.
180(2), 1152–1165.
mla: Oochi, A., et al. “Pinstatic Acid Promotes Auxin Transport by Inhibiting PIN
Internalization.” Plant Physiology, vol. 180, no. 2, ASPB, 2019, pp. 1152–65,
doi:10.1104/pp.19.00201.
short: A. Oochi, J. Hajny, K. Fukui, Y. Nakao, M.C. Gallei, M. Quareshy, K. Takahashi,
T. Kinoshita, S. Harborough, S. Kepinski, H. Kasahara, R. Napier, J. Friml, K.
Hayashi, Plant Physiology 180 (2019) 1152–1165.
date_created: 2019-04-09T08:38:20Z
date_published: 2019-06-01T00:00:00Z
date_updated: 2024-03-18T23:30:39Z
day: '01'
department:
- _id: JiFr
doi: 10.1104/pp.19.00201
ec_funded: 1
external_id:
isi:
- '000470086100045'
pmid:
- '30936248'
intvolume: ' 180'
isi: 1
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1104/pp.19.00201
month: '06'
oa: 1
oa_version: Published Version
page: 1152-1165
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Plant Physiology
publication_identifier:
eissn:
- 1532-2548
issn:
- 0032-0889
publication_status: published
publisher: ASPB
quality_controlled: '1'
related_material:
record:
- id: '11626'
relation: dissertation_contains
status: public
- id: '8822'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Pinstatic acid promotes auxin transport by inhibiting PIN internalization
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 180
year: '2019'
...
---
_id: '6627'
abstract:
- lang: eng
text: Cortical microtubule arrays in elongating epidermal cells in both the root
and stem of plants have the propensity of dynamic reorientations that are correlated
with the activation or inhibition of growth. Factors regulating plant growth,
among them the hormone auxin, have been recognized as regulators of microtubule
array orientations. Some previous work in the field has aimed at elucidating the
causal relationship between cell growth, the signaling of auxin or other growth-regulating
factors, and microtubule array reorientations, with various conclusions. Here,
we revisit this problem of causality with a comprehensive set of experiments in
Arabidopsis thaliana, using the now available pharmacological and genetic tools.
We use isolated, auxin-depleted hypocotyls, an experimental system allowing for
full control of both growth and auxin signaling. We demonstrate that reorientation
of microtubules is not directly triggered by an auxin signal during growth activation.
Instead, reorientation is triggered by the activation of the growth process itself
and is auxin-independent in its nature. We discuss these findings in the context
of previous relevant work, including that on the mechanical regulation of microtubule
array orientation.
article_number: '3337'
article_processing_charge: Yes
article_type: original
author:
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Adamowski M, Li L, Friml J. Reorientation of cortical microtubule arrays in
the hypocotyl of arabidopsis thaliana is induced by the cell growth process and
independent of auxin signaling. International Journal of Molecular Sciences.
2019;20(13). doi:10.3390/ijms20133337
apa: Adamowski, M., Li, L., & Friml, J. (2019). Reorientation of cortical microtubule
arrays in the hypocotyl of arabidopsis thaliana is induced by the cell growth
process and independent of auxin signaling. International Journal of Molecular
Sciences. MDPI. https://doi.org/10.3390/ijms20133337
chicago: Adamowski, Maciek, Lanxin Li, and Jiří Friml. “Reorientation of Cortical
Microtubule Arrays in the Hypocotyl of Arabidopsis Thaliana Is Induced by the
Cell Growth Process and Independent of Auxin Signaling.” International Journal
of Molecular Sciences. MDPI, 2019. https://doi.org/10.3390/ijms20133337.
ieee: M. Adamowski, L. Li, and J. Friml, “Reorientation of cortical microtubule
arrays in the hypocotyl of arabidopsis thaliana is induced by the cell growth
process and independent of auxin signaling,” International Journal of Molecular
Sciences, vol. 20, no. 13. MDPI, 2019.
ista: Adamowski M, Li L, Friml J. 2019. Reorientation of cortical microtubule arrays
in the hypocotyl of arabidopsis thaliana is induced by the cell growth process
and independent of auxin signaling. International Journal of Molecular Sciences.
20(13), 3337.
mla: Adamowski, Maciek, et al. “Reorientation of Cortical Microtubule Arrays in
the Hypocotyl of Arabidopsis Thaliana Is Induced by the Cell Growth Process and
Independent of Auxin Signaling.” International Journal of Molecular Sciences,
vol. 20, no. 13, 3337, MDPI, 2019, doi:10.3390/ijms20133337.
short: M. Adamowski, L. Li, J. Friml, International Journal of Molecular Sciences
20 (2019).
date_created: 2019-07-11T12:00:32Z
date_published: 2019-07-07T00:00:00Z
date_updated: 2024-03-18T23:30:45Z
day: '07'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.3390/ijms20133337
ec_funded: 1
external_id:
isi:
- '000477041100221'
pmid:
- '31284661'
file:
- access_level: open_access
checksum: dd9d1cbb933a72ceb666c9667890ac51
content_type: application/pdf
creator: dernst
date_created: 2019-07-17T06:17:15Z
date_updated: 2020-07-14T12:47:34Z
file_id: '6645'
file_name: 2019_JournalMolecularScience_Adamowski.pdf
file_size: 3330291
relation: main_file
file_date_updated: 2020-07-14T12:47:34Z
has_accepted_license: '1'
intvolume: ' 20'
isi: 1
issue: '13'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
- _id: B67AFEDC-15C9-11EA-A837-991A96BB2854
name: IST Austria Open Access Fund
publication: International Journal of Molecular Sciences
publication_identifier:
eissn:
- 1422-0067
publication_status: published
publisher: MDPI
quality_controlled: '1'
related_material:
record:
- id: '10083'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Reorientation of cortical microtubule arrays in the hypocotyl of arabidopsis
thaliana is induced by the cell growth process and independent of auxin signaling
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 20
year: '2019'
...
---
_id: '408'
abstract:
- lang: eng
text: Adventitious roots (AR) are de novo formed roots that emerge from any part
of the plant or from callus in tissue culture, except root tissue. The plant tissue
origin and the method by which they are induced determine the physiological properties
of emerged ARs. Hence, a standard method encompassing all types of AR does not
exist. Here we describe a method for the induction and analysis of AR that emerge
from the etiolated hypocotyl of dicot plants. The hypocotyl is formed during embryogenesis
and shows a determined developmental pattern which usually does not involve AR
formation. However, the hypocotyl shows propensity to form de novo roots under
specific circumstances such as removal of the root system, high humidity or flooding,
or during de-etiolation. The hypocotyl AR emerge from a pericycle-like cell layer
surrounding the vascular tissue of the central cylinder, which is reminiscent
to the developmental program of lateral roots. Here we propose an easy protocol
for in vitro hypocotyl AR induction from etiolated Arabidopsis seedlings.
alternative_title:
- MIMB
article_processing_charge: No
author:
- first_name: Hoang
full_name: Trinh, Hoang
last_name: Trinh
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Danny
full_name: Geelen, Danny
last_name: Geelen
citation:
ama: 'Trinh H, Verstraeten I, Geelen D. In vitro assay for induction of adventitious
rooting on intact arabidopsis hypocotyls. In: Root Development . Vol 1761.
Springer Nature; 2018:95-102. doi:10.1007/978-1-4939-7747-5_7'
apa: Trinh, H., Verstraeten, I., & Geelen, D. (2018). In vitro assay for induction
of adventitious rooting on intact arabidopsis hypocotyls. In Root Development
(Vol. 1761, pp. 95–102). Springer Nature. https://doi.org/10.1007/978-1-4939-7747-5_7
chicago: Trinh, Hoang, Inge Verstraeten, and Danny Geelen. “In Vitro Assay for Induction
of Adventitious Rooting on Intact Arabidopsis Hypocotyls.” In Root Development
, 1761:95–102. Springer Nature, 2018. https://doi.org/10.1007/978-1-4939-7747-5_7.
ieee: H. Trinh, I. Verstraeten, and D. Geelen, “In vitro assay for induction of
adventitious rooting on intact arabidopsis hypocotyls,” in Root Development
, vol. 1761, Springer Nature, 2018, pp. 95–102.
ista: 'Trinh H, Verstraeten I, Geelen D. 2018.In vitro assay for induction of adventitious
rooting on intact arabidopsis hypocotyls. In: Root Development . MIMB, vol. 1761,
95–102.'
mla: Trinh, Hoang, et al. “In Vitro Assay for Induction of Adventitious Rooting
on Intact Arabidopsis Hypocotyls.” Root Development , vol. 1761, Springer
Nature, 2018, pp. 95–102, doi:10.1007/978-1-4939-7747-5_7.
short: H. Trinh, I. Verstraeten, D. Geelen, in:, Root Development , Springer Nature,
2018, pp. 95–102.
date_created: 2018-12-11T11:46:18Z
date_published: 2018-03-01T00:00:00Z
date_updated: 2021-01-12T07:54:21Z
day: '01'
department:
- _id: JiFr
doi: 10.1007/978-1-4939-7747-5_7
external_id:
pmid:
- '29525951'
intvolume: ' 1761'
language:
- iso: eng
month: '03'
oa_version: None
page: 95 - 102
pmid: 1
publication: 'Root Development '
publication_identifier:
issn:
- 1064-3745
publication_status: published
publisher: Springer Nature
publist_id: '7421'
quality_controlled: '1'
scopus_import: '1'
status: public
title: In vitro assay for induction of adventitious rooting on intact arabidopsis
hypocotyls
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 1761
year: '2018'
...
---
_id: '411'
abstract:
- lang: eng
text: Immunolocalization is a valuable tool for cell biology research that allows
to rapidly determine the localization and expression levels of endogenous proteins.
In plants, whole-mount in situ immunolocalization remains a challenging method,
especially in tissues protected by waxy layers and complex cell wall carbohydrates.
Here, we present a robust method for whole-mount in situ immunolocalization in
primary root meristems and lateral root primordia in Arabidopsis thaliana. For
good epitope preservation, fixation is done in an alkaline paraformaldehyde/glutaraldehyde
mixture. This fixative is suitable for detecting a wide range of proteins, including
integral transmembrane proteins and proteins peripherally attached to the plasma
membrane. From initiation until emergence from the primary root, lateral root
primordia are surrounded by several layers of differentiated tissues with a complex
cell wall composition that interferes with the efficient penetration of all buffers.
Therefore, immunolocalization in early lateral root primordia requires a modified
method, including a strong solvent treatment for removal of hydrophobic barriers
and a specific cocktail of cell wall-degrading enzymes. The presented method allows
for easy, reliable, and high-quality in situ detection of the subcellular localization
of endogenous proteins in primary and lateral root meristems without the need
of time-consuming crosses or making translational fusions to fluorescent proteins.
alternative_title:
- Methods in Molecular Biology
author:
- first_name: Michael
full_name: Karampelias, Michael
last_name: Karampelias
- first_name: Ricardo
full_name: Tejos, Ricardo
last_name: Tejos
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
citation:
ama: 'Karampelias M, Tejos R, Friml J, Vanneste S. Optimized whole mount in situ
immunolocalization for Arabidopsis thaliana root meristems and lateral root primordia.
In: Ristova D, Barbez E, eds. Root Development. Methods and Protocols.
Vol 1761. MIMB. Springer; 2018:131-143. doi:10.1007/978-1-4939-7747-5_10'
apa: Karampelias, M., Tejos, R., Friml, J., & Vanneste, S. (2018). Optimized
whole mount in situ immunolocalization for Arabidopsis thaliana root meristems
and lateral root primordia. In D. Ristova & E. Barbez (Eds.), Root Development.
Methods and Protocols (Vol. 1761, pp. 131–143). Springer. https://doi.org/10.1007/978-1-4939-7747-5_10
chicago: Karampelias, Michael, Ricardo Tejos, Jiří Friml, and Steffen Vanneste.
“Optimized Whole Mount in Situ Immunolocalization for Arabidopsis Thaliana Root
Meristems and Lateral Root Primordia.” In Root Development. Methods and Protocols,
edited by Daniela Ristova and Elke Barbez, 1761:131–43. MIMB. Springer, 2018.
https://doi.org/10.1007/978-1-4939-7747-5_10.
ieee: M. Karampelias, R. Tejos, J. Friml, and S. Vanneste, “Optimized whole mount
in situ immunolocalization for Arabidopsis thaliana root meristems and lateral
root primordia,” in Root Development. Methods and Protocols, vol. 1761,
D. Ristova and E. Barbez, Eds. Springer, 2018, pp. 131–143.
ista: 'Karampelias M, Tejos R, Friml J, Vanneste S. 2018.Optimized whole mount in
situ immunolocalization for Arabidopsis thaliana root meristems and lateral root
primordia. In: Root Development. Methods and Protocols. Methods in Molecular Biology,
vol. 1761, 131–143.'
mla: Karampelias, Michael, et al. “Optimized Whole Mount in Situ Immunolocalization
for Arabidopsis Thaliana Root Meristems and Lateral Root Primordia.” Root
Development. Methods and Protocols, edited by Daniela Ristova and Elke Barbez,
vol. 1761, Springer, 2018, pp. 131–43, doi:10.1007/978-1-4939-7747-5_10.
short: M. Karampelias, R. Tejos, J. Friml, S. Vanneste, in:, D. Ristova, E. Barbez
(Eds.), Root Development. Methods and Protocols, Springer, 2018, pp. 131–143.
date_created: 2018-12-11T11:46:20Z
date_published: 2018-03-11T00:00:00Z
date_updated: 2021-01-12T07:54:34Z
day: '11'
department:
- _id: JiFr
doi: 10.1007/978-1-4939-7747-5_10
editor:
- first_name: Daniela
full_name: Ristova, Daniela
last_name: Ristova
- first_name: Elke
full_name: Barbez, Elke
last_name: Barbez
intvolume: ' 1761'
language:
- iso: eng
month: '03'
oa_version: None
page: 131 - 143
publication: Root Development. Methods and Protocols
publication_status: published
publisher: Springer
publist_id: '7418'
quality_controlled: '1'
scopus_import: 1
series_title: MIMB
status: public
title: Optimized whole mount in situ immunolocalization for Arabidopsis thaliana root
meristems and lateral root primordia
type: book_chapter
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 1761
year: '2018'
...
---
_id: '203'
abstract:
- lang: eng
text: Asymmetric auxin distribution is instrumental for the differential growth
that causes organ bending on tropic stimuli and curvatures during plant development.
Local differences in auxin concentrations are achieved mainly by polarized cellular
distribution of PIN auxin transporters, but whether other mechanisms involving
auxin homeostasis are also relevant for the formation of auxin gradients is not
clear. Here we show that auxin methylation is required for asymmetric auxin distribution
across the hypocotyl, particularly during its response to gravity. We found that
loss-of-function mutants in Arabidopsis IAA CARBOXYL METHYLTRANSFERASE1 (IAMT1)
prematurely unfold the apical hook, and that their hypocotyls are impaired in
gravitropic reorientation. This defect is linked to an auxin-dependent increase
in PIN gene expression, leading to an increased polar auxin transport and lack
of asymmetric distribution of PIN3 in the iamt1 mutant. Gravitropic reorientation
in the iamt1 mutant could be restored with either endodermis-specific expression
of IAMT1 or partial inhibition of polar auxin transport, which also results in
normal PIN gene expression levels. We propose that IAA methylation is necessary
in gravity-sensing cells to restrict polar auxin transport within the range of
auxin levels that allow for differential responses.
article_processing_charge: No
author:
- first_name: Mohamad
full_name: Abbas, Mohamad
id: 47E8FC1C-F248-11E8-B48F-1D18A9856A87
last_name: Abbas
- first_name: García J
full_name: Hernández, García J
last_name: Hernández
- first_name: Stephan
full_name: Pollmann, Stephan
last_name: Pollmann
- first_name: Sophia L
full_name: Samodelov, Sophia L
last_name: Samodelov
- first_name: Martina
full_name: Kolb, Martina
last_name: Kolb
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Ulrich Z
full_name: Hammes, Ulrich Z
last_name: Hammes
- first_name: Matias D
full_name: Zurbriggen, Matias D
last_name: Zurbriggen
- first_name: Miguel
full_name: Blázquez, Miguel
last_name: Blázquez
- first_name: David
full_name: Alabadí, David
last_name: Alabadí
citation:
ama: Abbas M, Hernández GJ, Pollmann S, et al. Auxin methylation is required for
differential growth in Arabidopsis. PNAS. 2018;115(26):6864-6869. doi:10.1073/pnas.1806565115
apa: Abbas, M., Hernández, G. J., Pollmann, S., Samodelov, S. L., Kolb, M., Friml,
J., … Alabadí, D. (2018). Auxin methylation is required for differential growth
in Arabidopsis. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1806565115
chicago: Abbas, Mohamad, García J Hernández, Stephan Pollmann, Sophia L Samodelov,
Martina Kolb, Jiří Friml, Ulrich Z Hammes, Matias D Zurbriggen, Miguel Blázquez,
and David Alabadí. “Auxin Methylation Is Required for Differential Growth in Arabidopsis.”
PNAS. National Academy of Sciences, 2018. https://doi.org/10.1073/pnas.1806565115.
ieee: M. Abbas et al., “Auxin methylation is required for differential growth
in Arabidopsis,” PNAS, vol. 115, no. 26. National Academy of Sciences,
pp. 6864–6869, 2018.
ista: Abbas M, Hernández GJ, Pollmann S, Samodelov SL, Kolb M, Friml J, Hammes UZ,
Zurbriggen MD, Blázquez M, Alabadí D. 2018. Auxin methylation is required for
differential growth in Arabidopsis. PNAS. 115(26), 6864–6869.
mla: Abbas, Mohamad, et al. “Auxin Methylation Is Required for Differential Growth
in Arabidopsis.” PNAS, vol. 115, no. 26, National Academy of Sciences,
2018, pp. 6864–69, doi:10.1073/pnas.1806565115.
short: M. Abbas, G.J. Hernández, S. Pollmann, S.L. Samodelov, M. Kolb, J. Friml,
U.Z. Hammes, M.D. Zurbriggen, M. Blázquez, D. Alabadí, PNAS 115 (2018) 6864–6869.
date_created: 2018-12-11T11:45:11Z
date_published: 2018-06-26T00:00:00Z
date_updated: 2023-09-08T13:24:40Z
day: '26'
department:
- _id: JiFr
doi: 10.1073/pnas.1806565115
ec_funded: 1
external_id:
isi:
- '000436245000096'
intvolume: ' 115'
isi: 1
issue: '26'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://eprints.nottingham.ac.uk/52388/
month: '06'
oa: 1
oa_version: None
page: 6864-6869
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '7710'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Auxin methylation is required for differential growth in Arabidopsis
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 115
year: '2018'
...
---
_id: '5830'
abstract:
- lang: eng
text: CLE peptides have been implicated in various developmental processes of plants
and mediate their responses to environmental stimuli. However, the biological
relevance of most CLE genes remains to be functionally characterized. Here, we
report that CLE9, which is expressed in stomata, acts as an essential regulator
in the induction of stomatal closure. Exogenous application of CLE9 peptides or
overexpression of CLE9 effectively led to stomatal closure and enhanced drought
tolerance, whereas CLE9 loss-of-function mutants were sensitivity to drought stress.
CLE9-induced stomatal closure was impaired in abscisic acid (ABA)-deficient mutants,
indicating that ABA is required for CLE9-medaited guard cell signalling. We further
deciphered that two guard cell ABA-signalling components, OST1 and SLAC1, were
responsible for CLE9-induced stomatal closure. MPK3 and MPK6 were activated by
the CLE9 peptide, and CLE9 peptides failed to close stomata in mpk3 and mpk6 mutants.
In addition, CLE9 peptides stimulated the induction of hydrogen peroxide (H2O2)
and nitric oxide (NO) synthesis associated with stomatal closure, which was abolished
in the NADPH oxidase-deficient mutants or nitric reductase mutants, respectively.
Collectively, our results reveal a novel ABA-dependent function of CLE9 in the
regulation of stomatal apertures, thereby suggesting a potential role of CLE9
in the stress acclimatization of plants.
article_processing_charge: No
author:
- first_name: Luosha
full_name: Zhang, Luosha
last_name: Zhang
- first_name: Xiong
full_name: Shi, Xiong
last_name: Shi
- first_name: Yutao
full_name: Zhang, Yutao
last_name: Zhang
- first_name: Jiajing
full_name: Wang, Jiajing
last_name: Wang
- first_name: Jingwei
full_name: Yang, Jingwei
last_name: Yang
- first_name: Takashi
full_name: Ishida, Takashi
last_name: Ishida
- first_name: Wenqian
full_name: Jiang, Wenqian
last_name: Jiang
- first_name: Xiangyu
full_name: Han, Xiangyu
last_name: Han
- first_name: Jingke
full_name: Kang, Jingke
last_name: Kang
- first_name: Xuening
full_name: Wang, Xuening
last_name: Wang
- first_name: Lixia
full_name: Pan, Lixia
last_name: Pan
- first_name: Shuo
full_name: Lv, Shuo
last_name: Lv
- first_name: Bing
full_name: Cao, Bing
last_name: Cao
- first_name: Yonghong
full_name: Zhang, Yonghong
last_name: Zhang
- first_name: Jinbin
full_name: Wu, Jinbin
last_name: Wu
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
- first_name: Zhubing
full_name: Hu, Zhubing
last_name: Hu
- first_name: Langjun
full_name: Cui, Langjun
last_name: Cui
- first_name: Shinichiro
full_name: Sawa, Shinichiro
last_name: Sawa
- first_name: Junmin
full_name: He, Junmin
last_name: He
- first_name: Guodong
full_name: Wang, Guodong
last_name: Wang
citation:
ama: Zhang L, Shi X, Zhang Y, et al. CLE9 peptide-induced stomatal closure is mediated
by abscisic acid, hydrogen peroxide, and nitric oxide in arabidopsis thaliana.
Plant Cell and Environment. 2018. doi:10.1111/pce.13475
apa: Zhang, L., Shi, X., Zhang, Y., Wang, J., Yang, J., Ishida, T., … Wang, G. (2018).
CLE9 peptide-induced stomatal closure is mediated by abscisic acid, hydrogen peroxide,
and nitric oxide in arabidopsis thaliana. Plant Cell and Environment. Wiley.
https://doi.org/10.1111/pce.13475
chicago: Zhang, Luosha, Xiong Shi, Yutao Zhang, Jiajing Wang, Jingwei Yang, Takashi
Ishida, Wenqian Jiang, et al. “CLE9 Peptide-Induced Stomatal Closure Is Mediated
by Abscisic Acid, Hydrogen Peroxide, and Nitric Oxide in Arabidopsis Thaliana.”
Plant Cell and Environment. Wiley, 2018. https://doi.org/10.1111/pce.13475.
ieee: L. Zhang et al., “CLE9 peptide-induced stomatal closure is mediated
by abscisic acid, hydrogen peroxide, and nitric oxide in arabidopsis thaliana,”
Plant Cell and Environment. Wiley, 2018.
ista: Zhang L, Shi X, Zhang Y, Wang J, Yang J, Ishida T, Jiang W, Han X, Kang J,
Wang X, Pan L, Lv S, Cao B, Zhang Y, Wu J, Han H, Hu Z, Cui L, Sawa S, He J, Wang
G. 2018. CLE9 peptide-induced stomatal closure is mediated by abscisic acid, hydrogen
peroxide, and nitric oxide in arabidopsis thaliana. Plant Cell and Environment.
mla: Zhang, Luosha, et al. “CLE9 Peptide-Induced Stomatal Closure Is Mediated by
Abscisic Acid, Hydrogen Peroxide, and Nitric Oxide in Arabidopsis Thaliana.” Plant
Cell and Environment, Wiley, 2018, doi:10.1111/pce.13475.
short: L. Zhang, X. Shi, Y. Zhang, J. Wang, J. Yang, T. Ishida, W. Jiang, X. Han,
J. Kang, X. Wang, L. Pan, S. Lv, B. Cao, Y. Zhang, J. Wu, H. Han, Z. Hu, L. Cui,
S. Sawa, J. He, G. Wang, Plant Cell and Environment (2018).
date_created: 2019-01-13T22:59:11Z
date_published: 2018-10-31T00:00:00Z
date_updated: 2023-09-11T12:43:31Z
day: '31'
department:
- _id: JiFr
doi: 10.1111/pce.13475
external_id:
isi:
- '000459014800021'
pmid:
- '30378140'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pubmed/30378140
month: '10'
oa: 1
oa_version: Published Version
pmid: 1
publication: Plant Cell and Environment
publication_identifier:
issn:
- '01407791'
publication_status: epub_ahead
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: CLE9 peptide-induced stomatal closure is mediated by abscisic acid, hydrogen
peroxide, and nitric oxide in arabidopsis thaliana
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2018'
...
---
_id: '428'
abstract:
- lang: eng
text: The plant hormone gibberellic acid (GA) is a crucial regulator of growth and
development. The main paradigm of GA signaling puts forward transcriptional regulation
via the degradation of DELLA transcriptional repressors. GA has also been shown
to regulate tropic responses by modulation of the plasma membrane incidence of
PIN auxin transporters by an unclear mechanism. Here we uncovered the cellular
and molecular mechanisms by which GA redirects protein trafficking and thus regulates
cell surface functionality. Photoconvertible reporters revealed that GA balances
the protein traffic between the vacuole degradation route and recycling back to
the cell surface. Low GA levels promote vacuolar delivery and degradation of multiple
cargos, including PIN proteins, whereas high GA levels promote their recycling
to the plasma membrane. This GA effect requires components of the retromer complex,
such as Sorting Nexin 1 (SNX1) and its interacting, microtubule (MT)-associated
protein, the Cytoplasmic Linker-Associated Protein (CLASP1). Accordingly, GA regulates
the subcellular distribution of SNX1 and CLASP1, and the intact MT cytoskeleton
is essential for the GA effect on trafficking. This GA cellular action occurs
through DELLA proteins that regulate the MT and retromer presumably via their
interaction partners Prefoldins (PFDs). Our study identified a branching of the
GA signaling pathway at the level of DELLA proteins, which, in parallel to regulating
transcription, also target by a nontranscriptional mechanism the retromer complex
acting at the intersection of the degradation and recycling trafficking routes.
By this mechanism, GA can redirect receptors and transporters to the cell surface,
thus coregulating multiple processes, including PIN-dependent auxin fluxes during
tropic responses.
acknowledgement: "We gratefully acknowledge M. Blázquez (Instituto de Biología Molecular
y Celular de Plantas), M. Fendrych, C. Cuesta Moliner (Institute of Science and
Technology Austria), M. Vanstraelen, M. Nowack (Center for Plant Systems Biology,
Ghent), C. Luschnig (Universitat fur Bodenkultur Wien, Vienna), S. Simon (Central
European Institute of Technology, Brno), C. Sommerville (Carnegie Institution for
Science), and Y. Gu (Penn State University) for making available the materials used
in this study;\r\n...funding from the European Research Council (ERC) under the
European Union’s Seventh Framework Programme (FP7/2007-2013)/ERC Grant Agreement
282300.\r\nCC BY NC ND"
article_processing_charge: No
author:
- first_name: Yuliya
full_name: Salanenka, Yuliya
id: 46DAAE7E-F248-11E8-B48F-1D18A9856A87
last_name: Salanenka
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Christian
full_name: Löfke, Christian
last_name: Löfke
- first_name: Kaori
full_name: Tabata, Kaori
id: 7DAAEDA4-02D0-11E9-B11A-A5A4D7DFFFD0
last_name: Tabata
- first_name: Satoshi
full_name: Naramoto, Satoshi
last_name: Naramoto
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Salanenka Y, Verstraeten I, Löfke C, et al. Gibberellin DELLA signaling targets
the retromer complex to redirect protein trafficking to the plasma membrane. PNAS.
2018;115(14):3716-3721. doi:10.1073/pnas.1721760115
apa: Salanenka, Y., Verstraeten, I., Löfke, C., Tabata, K., Naramoto, S., Glanc,
M., & Friml, J. (2018). Gibberellin DELLA signaling targets the retromer complex
to redirect protein trafficking to the plasma membrane. PNAS. National
Academy of Sciences. https://doi.org/10.1073/pnas.1721760115
chicago: Salanenka, Yuliya, Inge Verstraeten, Christian Löfke, Kaori Tabata, Satoshi
Naramoto, Matous Glanc, and Jiří Friml. “Gibberellin DELLA Signaling Targets the
Retromer Complex to Redirect Protein Trafficking to the Plasma Membrane.” PNAS.
National Academy of Sciences, 2018. https://doi.org/10.1073/pnas.1721760115.
ieee: Y. Salanenka et al., “Gibberellin DELLA signaling targets the retromer
complex to redirect protein trafficking to the plasma membrane,” PNAS,
vol. 115, no. 14. National Academy of Sciences, pp. 3716–3721, 2018.
ista: Salanenka Y, Verstraeten I, Löfke C, Tabata K, Naramoto S, Glanc M, Friml
J. 2018. Gibberellin DELLA signaling targets the retromer complex to redirect
protein trafficking to the plasma membrane. PNAS. 115(14), 3716–3721.
mla: Salanenka, Yuliya, et al. “Gibberellin DELLA Signaling Targets the Retromer
Complex to Redirect Protein Trafficking to the Plasma Membrane.” PNAS,
vol. 115, no. 14, National Academy of Sciences, 2018, pp. 3716–21, doi:10.1073/pnas.1721760115.
short: Y. Salanenka, I. Verstraeten, C. Löfke, K. Tabata, S. Naramoto, M. Glanc,
J. Friml, PNAS 115 (2018) 3716–3721.
date_created: 2018-12-11T11:46:25Z
date_published: 2018-04-03T00:00:00Z
date_updated: 2023-09-11T14:06:34Z
day: '03'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1073/pnas.1721760115
ec_funded: 1
external_id:
isi:
- '000429012500073'
file:
- access_level: open_access
checksum: 1fcf7223fb8f99559cfa80bd6f24ce44
content_type: application/pdf
creator: dernst
date_created: 2018-12-17T12:30:14Z
date_updated: 2020-07-14T12:46:26Z
file_id: '5700'
file_name: 2018_PNAS_Salanenka.pdf
file_size: 1924101
relation: main_file
file_date_updated: 2020-07-14T12:46:26Z
has_accepted_license: '1'
intvolume: ' 115'
isi: 1
issue: '14'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
page: ' 3716 - 3721'
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '7395'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Gibberellin DELLA signaling targets the retromer complex to redirect protein
trafficking to the plasma membrane
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 115
year: '2018'
...
---
_id: '280'
abstract:
- lang: eng
text: Flowers have a species-specific functional life span that determines the time
window in which pollination, fertilization and seed set can occur. The stigma
tissue plays a key role in flower receptivity by intercepting pollen and initiating
pollen tube growth toward the ovary. In this article, we show that a developmentally
controlled cell death programme terminates the functional life span of stigma
cells in Arabidopsis. We identified the leaf senescence regulator ORESARA1 (also
known as ANAC092) and the previously uncharacterized KIRA1 (also known as ANAC074)
as partially redundant transcription factors that modulate stigma longevity by
controlling the expression of programmed cell death-associated genes. KIRA1 expression
is sufficient to induce cell death and terminate floral receptivity, whereas lack
of both KIRA1 and ORESARA1 substantially increases stigma life span. Surprisingly,
the extension of stigma longevity is accompanied by only a moderate extension
of flower receptivity, suggesting that additional processes participate in the
control of the flower's receptive life span.
acknowledgement: We gratefully acknowledge funding from the Chinese Scholarship Council
(CSC; project number 201206910025 to Z.G.), the Fonds Wetenschappelijk Onderzoek
(FWO; project number G005112N to A.D.; fellowship number 12I7417N to Z.L.), the
Belgian Federal Science Policy Office (BELSPO; to Y.S.), the Agency for Innovation
by Science and Technology of Belgium (IWT; fellowship number 121110 to M.V.D.),
the Hercules foundation (grant AUGE-09-029 to K.D.), and the ERC StG PROCELLDEATH
(project number 639234 to M.K.N.).
article_processing_charge: No
author:
- first_name: Zhen
full_name: Gao, Zhen
last_name: Gao
- first_name: Anna
full_name: Daneva, Anna
last_name: Daneva
- first_name: Yuliya
full_name: Salanenka, Yuliya
id: 46DAAE7E-F248-11E8-B48F-1D18A9856A87
last_name: Salanenka
- first_name: Matthias
full_name: Van Durme, Matthias
last_name: Van Durme
- first_name: Marlies
full_name: Huysmans, Marlies
last_name: Huysmans
- first_name: Zongcheng
full_name: Lin, Zongcheng
last_name: Lin
- first_name: Freya
full_name: De Winter, Freya
last_name: De Winter
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Mansour
full_name: Karimi, Mansour
last_name: Karimi
- first_name: Jan
full_name: Van De Velde, Jan
last_name: Van De Velde
- first_name: Klaas
full_name: Vandepoele, Klaas
last_name: Vandepoele
- first_name: Davy
full_name: Van De Walle, Davy
last_name: Van De Walle
- first_name: Koen
full_name: Dewettinck, Koen
last_name: Dewettinck
- first_name: Bart
full_name: Lambrecht, Bart
last_name: Lambrecht
- first_name: Moritz
full_name: Nowack, Moritz
last_name: Nowack
citation:
ama: Gao Z, Daneva A, Salanenka Y, et al. KIRA1 and ORESARA1 terminate flower receptivity
by promoting cell death in the stigma of Arabidopsis. Nature Plants. 2018;4(6):365-375.
doi:10.1038/s41477-018-0160-7
apa: Gao, Z., Daneva, A., Salanenka, Y., Van Durme, M., Huysmans, M., Lin, Z., …
Nowack, M. (2018). KIRA1 and ORESARA1 terminate flower receptivity by promoting
cell death in the stigma of Arabidopsis. Nature Plants. Nature Publishing
Group. https://doi.org/10.1038/s41477-018-0160-7
chicago: Gao, Zhen, Anna Daneva, Yuliya Salanenka, Matthias Van Durme, Marlies Huysmans,
Zongcheng Lin, Freya De Winter, et al. “KIRA1 and ORESARA1 Terminate Flower Receptivity
by Promoting Cell Death in the Stigma of Arabidopsis.” Nature Plants. Nature
Publishing Group, 2018. https://doi.org/10.1038/s41477-018-0160-7.
ieee: Z. Gao et al., “KIRA1 and ORESARA1 terminate flower receptivity by
promoting cell death in the stigma of Arabidopsis,” Nature Plants, vol.
4, no. 6. Nature Publishing Group, pp. 365–375, 2018.
ista: Gao Z, Daneva A, Salanenka Y, Van Durme M, Huysmans M, Lin Z, De Winter F,
Vanneste S, Karimi M, Van De Velde J, Vandepoele K, Van De Walle D, Dewettinck
K, Lambrecht B, Nowack M. 2018. KIRA1 and ORESARA1 terminate flower receptivity
by promoting cell death in the stigma of Arabidopsis. Nature Plants. 4(6), 365–375.
mla: Gao, Zhen, et al. “KIRA1 and ORESARA1 Terminate Flower Receptivity by Promoting
Cell Death in the Stigma of Arabidopsis.” Nature Plants, vol. 4, no. 6,
Nature Publishing Group, 2018, pp. 365–75, doi:10.1038/s41477-018-0160-7.
short: Z. Gao, A. Daneva, Y. Salanenka, M. Van Durme, M. Huysmans, Z. Lin, F. De
Winter, S. Vanneste, M. Karimi, J. Van De Velde, K. Vandepoele, D. Van De Walle,
K. Dewettinck, B. Lambrecht, M. Nowack, Nature Plants 4 (2018) 365–375.
date_created: 2018-12-11T11:45:35Z
date_published: 2018-05-28T00:00:00Z
date_updated: 2023-09-13T08:24:17Z
day: '28'
department:
- _id: JiFr
doi: 10.1038/s41477-018-0160-7
external_id:
isi:
- '000435571000017'
intvolume: ' 4'
isi: 1
issue: '6'
language:
- iso: eng
month: '05'
oa_version: None
page: 365 - 375
publication: Nature Plants
publication_status: published
publisher: Nature Publishing Group
publist_id: '7619'
quality_controlled: '1'
scopus_import: '1'
status: public
title: KIRA1 and ORESARA1 terminate flower receptivity by promoting cell death in
the stigma of Arabidopsis
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 4
year: '2018'
...
---
_id: '158'
abstract:
- lang: eng
text: 'The angiosperm seed is composed of three genetically distinct tissues: the
diploid embryo that originates from the fertilized egg cell, the triploid endosperm
that is produced from the fertilized central cell, and the maternal sporophytic
integuments that develop into the seed coat1. At the onset of embryo development
in Arabidopsis thaliana, the zygote divides asymmetrically, producing a small
apical embryonic cell and a larger basal cell that connects the embryo to the
maternal tissue2. The coordinated and synchronous development of the embryo and
the surrounding integuments, and the alignment of their growth axes, suggest communication
between maternal tissues and the embryo. In contrast to animals, however, where
a network of maternal factors that direct embryo patterning have been identified3,4,
only a few maternal mutations have been described to affect embryo development
in plants5–7. Early embryo patterning in Arabidopsis requires accumulation of
the phytohormone auxin in the apical cell by directed transport from the suspensor8–10.
However, the origin of this auxin has remained obscure. Here we investigate the
source of auxin for early embryogenesis and provide evidence that the mother plant
coordinates seed development by supplying auxin to the early embryo from the integuments
of the ovule. We show that auxin response increases in ovules after fertilization,
due to upregulated auxin biosynthesis in the integuments, and this maternally
produced auxin is required for correct embryo development.'
acknowledgement: This work was further supported by the Czech Science Foundation GACR
(GA13-40637S) to J.F.;
article_processing_charge: No
author:
- first_name: Hélène
full_name: Robert, Hélène
last_name: Robert
- first_name: Chulmin
full_name: Park, Chulmin
last_name: Park
- first_name: Carla
full_name: Gutièrrez, Carla
last_name: Gutièrrez
- first_name: Barbara
full_name: Wójcikowska, Barbara
last_name: Wójcikowska
- first_name: Aleš
full_name: Pěnčík, Aleš
last_name: Pěnčík
- first_name: Ondřej
full_name: Novák, Ondřej
last_name: Novák
- first_name: Junyi
full_name: Chen, Junyi
last_name: Chen
- first_name: Wim
full_name: Grunewald, Wim
last_name: Grunewald
- first_name: Thomas
full_name: Dresselhaus, Thomas
last_name: Dresselhaus
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Thomas
full_name: Laux, Thomas
last_name: Laux
citation:
ama: Robert H, Park C, Gutièrrez C, et al. Maternal auxin supply contributes to
early embryo patterning in Arabidopsis. Nature Plants. 2018;4(8):548-553.
doi:10.1038/s41477-018-0204-z
apa: Robert, H., Park, C., Gutièrrez, C., Wójcikowska, B., Pěnčík, A., Novák, O.,
… Laux, T. (2018). Maternal auxin supply contributes to early embryo patterning
in Arabidopsis. Nature Plants. Nature Publishing Group. https://doi.org/10.1038/s41477-018-0204-z
chicago: Robert, Hélène, Chulmin Park, Carla Gutièrrez, Barbara Wójcikowska, Aleš
Pěnčík, Ondřej Novák, Junyi Chen, et al. “Maternal Auxin Supply Contributes to
Early Embryo Patterning in Arabidopsis.” Nature Plants. Nature Publishing
Group, 2018. https://doi.org/10.1038/s41477-018-0204-z.
ieee: H. Robert et al., “Maternal auxin supply contributes to early embryo
patterning in Arabidopsis,” Nature Plants, vol. 4, no. 8. Nature Publishing
Group, pp. 548–553, 2018.
ista: Robert H, Park C, Gutièrrez C, Wójcikowska B, Pěnčík A, Novák O, Chen J, Grunewald
W, Dresselhaus T, Friml J, Laux T. 2018. Maternal auxin supply contributes to
early embryo patterning in Arabidopsis. Nature Plants. 4(8), 548–553.
mla: Robert, Hélène, et al. “Maternal Auxin Supply Contributes to Early Embryo Patterning
in Arabidopsis.” Nature Plants, vol. 4, no. 8, Nature Publishing Group,
2018, pp. 548–53, doi:10.1038/s41477-018-0204-z.
short: H. Robert, C. Park, C. Gutièrrez, B. Wójcikowska, A. Pěnčík, O. Novák, J.
Chen, W. Grunewald, T. Dresselhaus, J. Friml, T. Laux, Nature Plants 4 (2018)
548–553.
date_created: 2018-12-11T11:44:56Z
date_published: 2018-07-16T00:00:00Z
date_updated: 2023-09-13T08:53:28Z
day: '16'
department:
- _id: JiFr
doi: 10.1038/s41477-018-0204-z
ec_funded: 1
external_id:
isi:
- '000443861300011'
pmid:
- '30013211'
intvolume: ' 4'
isi: 1
issue: '8'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pubmed/30013211
month: '07'
oa: 1
oa_version: Submitted Version
page: 548 - 553
pmid: 1
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Nature Plants
publication_status: published
publisher: Nature Publishing Group
publist_id: '7763'
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/plant-mothers-talk-to-their-embryos-via-the-hormone-auxin/
scopus_import: '1'
status: public
title: Maternal auxin supply contributes to early embryo patterning in Arabidopsis
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 4
year: '2018'
...
---
_id: '462'
abstract:
- lang: eng
text: 'AtNHX5 and AtNHX6 are endosomal Na+,K+/H+ antiporters that are critical for
growth and development in Arabidopsis, but the mechanism behind their action remains
unknown. Here, we report that AtNHX5 and AtNHX6, functioning as H+ leak, control
auxin homeostasis and auxin-mediated development. We found that nhx5 nhx6 exhibited
growth variations of auxin-related defects. We further showed that nhx5 nhx6 was
affected in auxin homeostasis. Genetic analysis showed that AtNHX5 and AtNHX6
were required for the function of the ER-localized auxin transporter PIN5. Although
AtNHX5 and AtNHX6 were co-localized with PIN5 at ER, they did not interact directly.
Instead, the conserved acidic residues in AtNHX5 and AtNHX6, which are essential
for exchange activity, were required for PIN5 function. AtNHX5 and AtNHX6 regulated
the pH in ER. Overall, AtNHX5 and AtNHX6 may regulate auxin transport across the
ER via the pH gradient created by their transport activity. H+-leak pathway provides
a fine-tuning mechanism that controls cellular auxin fluxes. '
acknowledgement: 'This work was supported by the National Natural Science Foundation
of China (31571464, 31371438 and 31070222 to Q.S.Q.), the National Basic Research
Program of China (973 project, 2013CB429904 to Q.S.Q.), the Research Fund for the
Doctoral Program of Higher Education of China (20130211110001 to Q.S.Q.), the Ministry
of Education, Youth and Sports of the Czech Republic (the National Program for Sustainability
I, LO1204), and The Czech Science Foundation GAČR (GA13–40637S) to JF. We thank
Dr. Tom J. Guilfoyle for DR5::GUS line and Dr. Jia Li for pBIB‐RFP vector and DR5::GFP
line. We thank Liping Guan and Yang Zhao for their help with the confocal microscope
assay. '
article_processing_charge: No
article_type: original
author:
- first_name: Ligang
full_name: Fan, Ligang
last_name: Fan
- first_name: Lei
full_name: Zhao, Lei
last_name: Zhao
- first_name: Wei
full_name: Hu, Wei
last_name: Hu
- first_name: Weina
full_name: Li, Weina
last_name: Li
- first_name: Ondřej
full_name: Novák, Ondřej
last_name: Novák
- first_name: Miroslav
full_name: Strnad, Miroslav
last_name: Strnad
- first_name: Sibu
full_name: Simon, Sibu
id: 4542EF9A-F248-11E8-B48F-1D18A9856A87
last_name: Simon
orcid: 0000-0002-1998-6741
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Jinbo
full_name: Shen, Jinbo
last_name: Shen
- first_name: Liwen
full_name: Jiang, Liwen
last_name: Jiang
- first_name: Quan
full_name: Qiu, Quan
last_name: Qiu
citation:
ama: Fan L, Zhao L, Hu W, et al. NHX antiporters regulate the pH of endoplasmic
reticulum and auxin-mediated development. Plant, Cell and Environment.
2018;41:850-864. doi:10.1111/pce.13153
apa: Fan, L., Zhao, L., Hu, W., Li, W., Novák, O., Strnad, M., … Qiu, Q. (2018).
NHX antiporters regulate the pH of endoplasmic reticulum and auxin-mediated development.
Plant, Cell and Environment. Wiley-Blackwell. https://doi.org/10.1111/pce.13153
chicago: Fan, Ligang, Lei Zhao, Wei Hu, Weina Li, Ondřej Novák, Miroslav Strnad,
Sibu Simon, et al. “NHX Antiporters Regulate the PH of Endoplasmic Reticulum and
Auxin-Mediated Development.” Plant, Cell and Environment. Wiley-Blackwell,
2018. https://doi.org/10.1111/pce.13153.
ieee: L. Fan et al., “NHX antiporters regulate the pH of endoplasmic reticulum
and auxin-mediated development,” Plant, Cell and Environment, vol. 41.
Wiley-Blackwell, pp. 850–864, 2018.
ista: Fan L, Zhao L, Hu W, Li W, Novák O, Strnad M, Simon S, Friml J, Shen J, Jiang
L, Qiu Q. 2018. NHX antiporters regulate the pH of endoplasmic reticulum and auxin-mediated
development. Plant, Cell and Environment. 41, 850–864.
mla: Fan, Ligang, et al. “NHX Antiporters Regulate the PH of Endoplasmic Reticulum
and Auxin-Mediated Development.” Plant, Cell and Environment, vol. 41,
Wiley-Blackwell, 2018, pp. 850–64, doi:10.1111/pce.13153.
short: L. Fan, L. Zhao, W. Hu, W. Li, O. Novák, M. Strnad, S. Simon, J. Friml, J.
Shen, L. Jiang, Q. Qiu, Plant, Cell and Environment 41 (2018) 850–864.
date_created: 2018-12-11T11:46:36Z
date_published: 2018-05-01T00:00:00Z
date_updated: 2023-09-13T09:03:18Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/pce.13153
external_id:
isi:
- '000426870500012'
pmid:
- '29360148'
file:
- access_level: open_access
checksum: 6a20f843565f962cb20281cdf5e40914
content_type: application/pdf
creator: dernst
date_created: 2019-11-18T16:22:22Z
date_updated: 2020-07-14T12:46:32Z
file_id: '7042'
file_name: 2018_PlantCellEnv_Fan.pdf
file_size: 1937976
relation: main_file
file_date_updated: 2020-07-14T12:46:32Z
has_accepted_license: '1'
intvolume: ' 41'
isi: 1
language:
- iso: eng
month: '05'
oa: 1
oa_version: Submitted Version
page: 850 - 864
pmid: 1
publication: Plant, Cell and Environment
publication_status: published
publisher: Wiley-Blackwell
publist_id: '7359'
quality_controlled: '1'
scopus_import: '1'
status: public
title: NHX antiporters regulate the pH of endoplasmic reticulum and auxin-mediated
development
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 41
year: '2018'
...
---
_id: '192'
abstract:
- lang: eng
text: The phytohormone auxin is the information carrier in a plethora of developmental
and physiological processes in plants(1). It has been firmly established that
canonical, nuclear auxin signalling acts through regulation of gene transcription(2).
Here, we combined microfluidics, live imaging, genetic engineering and computational
modelling to reanalyse the classical case of root growth inhibition(3) by auxin.
We show that Arabidopsis roots react to addition and removal of auxin by extremely
rapid adaptation of growth rate. This process requires intracellular auxin perception
but not transcriptional reprogramming. The formation of the canonical TIR1/AFB-Aux/IAA
co-receptor complex is required for the growth regulation, hinting to a novel,
non-transcriptional branch of this signalling pathway. Our results challenge the
current understanding of root growth regulation by auxin and suggest another,
presumably non-transcriptional, signalling output of the canonical auxin pathway.
article_processing_charge: No
article_type: original
author:
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: Maria
full_name: Akhmanova, Maria
id: 3425EC26-F248-11E8-B48F-1D18A9856A87
last_name: Akhmanova
orcid: 0000-0003-1522-3162
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Matous
full_name: Glanc, Matous
last_name: Glanc
- first_name: Shinya
full_name: Hagihara, Shinya
last_name: Hagihara
- first_name: Koji
full_name: Takahashi, Koji
last_name: Takahashi
- first_name: Naoyuki
full_name: Uchida, Naoyuki
last_name: Uchida
- first_name: Keiko U
full_name: Torii, Keiko U
last_name: Torii
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Fendrych M, Akhmanova M, Merrin J, et al. Rapid and reversible root growth
inhibition by TIR1 auxin signalling. Nature Plants. 2018;4(7):453-459.
doi:10.1038/s41477-018-0190-1
apa: Fendrych, M., Akhmanova, M., Merrin, J., Glanc, M., Hagihara, S., Takahashi,
K., … Friml, J. (2018). Rapid and reversible root growth inhibition by TIR1 auxin
signalling. Nature Plants. Springer Nature. https://doi.org/10.1038/s41477-018-0190-1
chicago: Fendrych, Matyas, Maria Akhmanova, Jack Merrin, Matous Glanc, Shinya Hagihara,
Koji Takahashi, Naoyuki Uchida, Keiko U Torii, and Jiří Friml. “Rapid and Reversible
Root Growth Inhibition by TIR1 Auxin Signalling.” Nature Plants. Springer
Nature, 2018. https://doi.org/10.1038/s41477-018-0190-1.
ieee: M. Fendrych et al., “Rapid and reversible root growth inhibition by
TIR1 auxin signalling,” Nature Plants, vol. 4, no. 7. Springer Nature,
pp. 453–459, 2018.
ista: Fendrych M, Akhmanova M, Merrin J, Glanc M, Hagihara S, Takahashi K, Uchida
N, Torii KU, Friml J. 2018. Rapid and reversible root growth inhibition by TIR1
auxin signalling. Nature Plants. 4(7), 453–459.
mla: Fendrych, Matyas, et al. “Rapid and Reversible Root Growth Inhibition by TIR1
Auxin Signalling.” Nature Plants, vol. 4, no. 7, Springer Nature, 2018,
pp. 453–59, doi:10.1038/s41477-018-0190-1.
short: M. Fendrych, M. Akhmanova, J. Merrin, M. Glanc, S. Hagihara, K. Takahashi,
N. Uchida, K.U. Torii, J. Friml, Nature Plants 4 (2018) 453–459.
date_created: 2018-12-11T11:45:07Z
date_published: 2018-06-25T00:00:00Z
date_updated: 2023-09-15T12:11:03Z
day: '25'
department:
- _id: JiFr
- _id: DaSi
- _id: NanoFab
doi: 10.1038/s41477-018-0190-1
external_id:
isi:
- '000443221200017'
pmid:
- '29942048'
intvolume: ' 4'
isi: 1
issue: '7'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pubmed/29942048
month: '06'
oa: 1
oa_version: Submitted Version
page: 453 - 459
pmid: 1
publication: Nature Plants
publication_status: published
publisher: Springer Nature
publist_id: '7728'
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/new-mechanism-for-the-plant-hormone-auxin-discovered/
scopus_import: '1'
status: public
title: Rapid and reversible root growth inhibition by TIR1 auxin signalling
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 4
year: '2018'
...
---
_id: '14'
abstract:
- lang: eng
text: The intercellular transport of auxin is driven by PIN-formed (PIN) auxin efflux
carriers. PINs are localized at the plasma membrane (PM) and on constitutively
recycling endomembrane vesicles. Therefore, PINs can mediate auxin transport either
by direct translocation across the PM or by pumping auxin into secretory vesicles
(SVs), leading to its secretory release upon fusion with the PM. Which of these
two mechanisms dominates is a matter of debate. Here, we addressed the issue with
a mathematical modeling approach. We demonstrate that the efficiency of secretory
transport depends on SV size, half-life of PINs on the PM, pH, exocytosis frequency
and PIN density. 3D structured illumination microscopy (SIM) was used to determine
PIN density on the PM. Combining this data with published values of the other
parameters, we show that the transport activity of PINs in SVs would have to be
at least 1000× greater than on the PM in order to produce a comparable macroscopic
auxin transport. If both transport mechanisms operated simultaneously and PINs
were equally active on SVs and PM, the contribution of secretion to the total
auxin flux would be negligible. In conclusion, while secretory vesicle-mediated
transport of auxin is an intriguing and theoretically possible model, it is unlikely
to be a major mechanism of auxin transport inplanta.
acknowledgement: 'European Research Council (ERC): 742985 to Jiri Friml; M.A. was
supported by the Austrian Science Fund (FWF) (M2379-B28); AJ was supported by the
Austria Science Fund (FWF): I03630 to Jiri Friml.'
article_processing_charge: No
article_type: original
author:
- first_name: Sander
full_name: Hille, Sander
last_name: Hille
- first_name: Maria
full_name: Akhmanova, Maria
id: 3425EC26-F248-11E8-B48F-1D18A9856A87
last_name: Akhmanova
orcid: 0000-0003-1522-3162
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Hille S, Akhmanova M, Glanc M, Johnson AJ, Friml J. Relative contribution
of PIN-containing secretory vesicles and plasma membrane PINs to the directed
auxin transport: Theoretical estimation. International Journal of Molecular
Sciences. 2018;19(11). doi:10.3390/ijms19113566'
apa: 'Hille, S., Akhmanova, M., Glanc, M., Johnson, A. J., & Friml, J. (2018).
Relative contribution of PIN-containing secretory vesicles and plasma membrane
PINs to the directed auxin transport: Theoretical estimation. International
Journal of Molecular Sciences. MDPI. https://doi.org/10.3390/ijms19113566'
chicago: 'Hille, Sander, Maria Akhmanova, Matous Glanc, Alexander J Johnson, and
Jiří Friml. “Relative Contribution of PIN-Containing Secretory Vesicles and Plasma
Membrane PINs to the Directed Auxin Transport: Theoretical Estimation.” International
Journal of Molecular Sciences. MDPI, 2018. https://doi.org/10.3390/ijms19113566.'
ieee: 'S. Hille, M. Akhmanova, M. Glanc, A. J. Johnson, and J. Friml, “Relative
contribution of PIN-containing secretory vesicles and plasma membrane PINs to
the directed auxin transport: Theoretical estimation,” International Journal
of Molecular Sciences, vol. 19, no. 11. MDPI, 2018.'
ista: 'Hille S, Akhmanova M, Glanc M, Johnson AJ, Friml J. 2018. Relative contribution
of PIN-containing secretory vesicles and plasma membrane PINs to the directed
auxin transport: Theoretical estimation. International Journal of Molecular Sciences.
19(11).'
mla: 'Hille, Sander, et al. “Relative Contribution of PIN-Containing Secretory Vesicles
and Plasma Membrane PINs to the Directed Auxin Transport: Theoretical Estimation.”
International Journal of Molecular Sciences, vol. 19, no. 11, MDPI, 2018,
doi:10.3390/ijms19113566.'
short: S. Hille, M. Akhmanova, M. Glanc, A.J. Johnson, J. Friml, International Journal
of Molecular Sciences 19 (2018).
date_created: 2018-12-11T11:44:09Z
date_published: 2018-11-12T00:00:00Z
date_updated: 2023-09-18T08:09:32Z
day: '12'
ddc:
- '580'
department:
- _id: DaSi
- _id: JiFr
doi: 10.3390/ijms19113566
ec_funded: 1
external_id:
isi:
- '000451528500282'
file:
- access_level: open_access
checksum: e4b59c2599b0ca26ebf5b8434bcde94a
content_type: application/pdf
creator: dernst
date_created: 2018-12-17T16:04:11Z
date_updated: 2020-07-14T12:44:50Z
file_id: '5719'
file_name: 2018_IJMS_Hille.pdf
file_size: 2200593
relation: main_file
file_date_updated: 2020-07-14T12:44:50Z
has_accepted_license: '1'
intvolume: ' 19'
isi: 1
issue: '11'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: International Journal of Molecular Sciences
publication_identifier:
eissn:
- 1422-0067
publication_status: published
publisher: MDPI
publist_id: '8042'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Relative contribution of PIN-containing secretory vesicles and plasma membrane
PINs to the directed auxin transport: Theoretical estimation'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 19
year: '2018'
...
---
_id: '36'
abstract:
- lang: eng
text: Wheat (Triticum ssp.) is one of the most important human food sources. However,
this crop is very sensitive to temperature changes. Specifically, processes during
wheat leaf, flower, and seed development and photosynthesis, which all contribute
to the yield of this crop, are affected by high temperature. While this has to
some extent been investigated on physiological, developmental, and molecular levels,
very little is known about early signalling events associated with an increase
in temperature. Phosphorylation-mediated signalling mechanisms, which are quick
and dynamic, are associated with plant growth and development, also under abiotic
stress conditions. Therefore, we probed the impact of a short-term and mild increase
in temperature on the wheat leaf and spikelet phosphoproteome. In total, 3822
(containing 5178 phosphosites) and 5581 phosphopeptides (containing 7023 phosphosites)
were identified in leaf and spikelet samples, respectively. Following statistical
analysis, the resulting data set provides the scientific community with a first
large-scale plant phosphoproteome under the control of higher ambient temperature.
This community resource on the high temperature-mediated wheat phosphoproteome
will be valuable for future studies. Our analyses also revealed a core set of
common proteins between leaf and spikelet, suggesting some level of conserved
regulatory mechanisms. Furthermore, we observed temperature-regulated interconversion
of phosphoforms, which probably impacts protein activity.
acknowledgement: TZ is supported by a grant from the Chinese Scholarship Council.
article_processing_charge: No
author:
- first_name: Lam
full_name: Vu, Lam
last_name: Vu
- first_name: Tingting
full_name: Zhu, Tingting
last_name: Zhu
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Brigitte
full_name: Van De Cotte, Brigitte
last_name: Van De Cotte
- first_name: Kris
full_name: Gevaert, Kris
last_name: Gevaert
- first_name: Ive
full_name: De Smet, Ive
last_name: De Smet
citation:
ama: Vu L, Zhu T, Verstraeten I, Van De Cotte B, Gevaert K, De Smet I. Temperature-induced
changes in the wheat phosphoproteome reveal temperature-regulated interconversion
of phosphoforms. Journal of Experimental Botany. 2018;69(19):4609-4624.
doi:10.1093/jxb/ery204
apa: Vu, L., Zhu, T., Verstraeten, I., Van De Cotte, B., Gevaert, K., & De Smet,
I. (2018). Temperature-induced changes in the wheat phosphoproteome reveal temperature-regulated
interconversion of phosphoforms. Journal of Experimental Botany. Oxford
University Press. https://doi.org/10.1093/jxb/ery204
chicago: Vu, Lam, Tingting Zhu, Inge Verstraeten, Brigitte Van De Cotte, Kris Gevaert,
and Ive De Smet. “Temperature-Induced Changes in the Wheat Phosphoproteome Reveal
Temperature-Regulated Interconversion of Phosphoforms.” Journal of Experimental
Botany. Oxford University Press, 2018. https://doi.org/10.1093/jxb/ery204.
ieee: L. Vu, T. Zhu, I. Verstraeten, B. Van De Cotte, K. Gevaert, and I. De Smet,
“Temperature-induced changes in the wheat phosphoproteome reveal temperature-regulated
interconversion of phosphoforms,” Journal of Experimental Botany, vol.
69, no. 19. Oxford University Press, pp. 4609–4624, 2018.
ista: Vu L, Zhu T, Verstraeten I, Van De Cotte B, Gevaert K, De Smet I. 2018. Temperature-induced
changes in the wheat phosphoproteome reveal temperature-regulated interconversion
of phosphoforms. Journal of Experimental Botany. 69(19), 4609–4624.
mla: Vu, Lam, et al. “Temperature-Induced Changes in the Wheat Phosphoproteome Reveal
Temperature-Regulated Interconversion of Phosphoforms.” Journal of Experimental
Botany, vol. 69, no. 19, Oxford University Press, 2018, pp. 4609–24, doi:10.1093/jxb/ery204.
short: L. Vu, T. Zhu, I. Verstraeten, B. Van De Cotte, K. Gevaert, I. De Smet, Journal
of Experimental Botany 69 (2018) 4609–4624.
date_created: 2018-12-11T11:44:17Z
date_published: 2018-08-31T00:00:00Z
date_updated: 2023-09-19T10:00:46Z
day: '31'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.1093/jxb/ery204
external_id:
isi:
- '000443568700010'
file:
- access_level: open_access
checksum: 34cb0a1611588b75bd6f4913fb4e30f1
content_type: application/pdf
creator: dernst
date_created: 2018-12-18T09:47:51Z
date_updated: 2020-07-14T12:46:13Z
file_id: '5741'
file_name: 2018_JournalExperimBotany_Vu.pdf
file_size: 3359316
relation: main_file
file_date_updated: 2020-07-14T12:46:13Z
has_accepted_license: '1'
intvolume: ' 69'
isi: 1
issue: '19'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
page: 4609 - 4624
publication: Journal of Experimental Botany
publication_status: published
publisher: Oxford University Press
publist_id: '8019'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Temperature-induced changes in the wheat phosphoproteome reveal temperature-regulated
interconversion of phosphoforms
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 69
year: '2018'
...
---
_id: '148'
abstract:
- lang: eng
text: 'Land plants evolved from charophytic algae, among which Charophyceae possess
the most complex body plans. We present the genome of Chara braunii; comparison
of the genome to those of land plants identified evolutionary novelties for plant
terrestrialization and land plant heritage genes. C. braunii employs unique xylan
synthases for cell wall biosynthesis, a phragmoplast (cell separation) mechanism
similar to that of land plants, and many phytohormones. C. braunii plastids are
controlled via land-plant-like retrograde signaling, and transcriptional regulation
is more elaborate than in other algae. The morphological complexity of this organism
may result from expanded gene families, with three cases of particular note: genes
effecting tolerance to reactive oxygen species (ROS), LysM receptor-like kinases,
and transcription factors (TFs). Transcriptomic analysis of sexual reproductive
structures reveals intricate control by TFs, activity of the ROS gene network,
and the ancestral use of plant-like storage and stress protection proteins in
the zygote.'
acknowledgement: In-Data-Review
article_processing_charge: No
author:
- first_name: Tomoaki
full_name: Nishiyama, Tomoaki
last_name: Nishiyama
- first_name: Hidetoshi
full_name: Sakayama, Hidetoshi
last_name: Sakayama
- first_name: Jan
full_name: De Vries, Jan
last_name: De Vries
- first_name: Henrik
full_name: Buschmann, Henrik
last_name: Buschmann
- first_name: Denis
full_name: Saint Marcoux, Denis
last_name: Saint Marcoux
- first_name: Kristian
full_name: Ullrich, Kristian
last_name: Ullrich
- first_name: Fabian
full_name: Haas, Fabian
last_name: Haas
- first_name: Lisa
full_name: Vanderstraeten, Lisa
last_name: Vanderstraeten
- first_name: Dirk
full_name: Becker, Dirk
last_name: Becker
- first_name: Daniel
full_name: Lang, Daniel
last_name: Lang
- first_name: Stanislav
full_name: Vosolsobě, Stanislav
last_name: Vosolsobě
- first_name: Stephane
full_name: Rombauts, Stephane
last_name: Rombauts
- first_name: Per
full_name: Wilhelmsson, Per
last_name: Wilhelmsson
- first_name: Philipp
full_name: Janitza, Philipp
last_name: Janitza
- first_name: Ramona
full_name: Kern, Ramona
last_name: Kern
- first_name: Alexander
full_name: Heyl, Alexander
last_name: Heyl
- first_name: Florian
full_name: Rümpler, Florian
last_name: Rümpler
- first_name: Luz
full_name: Calderón Villalobos, Luz
last_name: Calderón Villalobos
- first_name: John
full_name: Clay, John
last_name: Clay
- first_name: Roman
full_name: Skokan, Roman
last_name: Skokan
- first_name: Atsushi
full_name: Toyoda, Atsushi
last_name: Toyoda
- first_name: Yutaka
full_name: Suzuki, Yutaka
last_name: Suzuki
- first_name: Hiroshi
full_name: Kagoshima, Hiroshi
last_name: Kagoshima
- first_name: Elio
full_name: Schijlen, Elio
last_name: Schijlen
- first_name: Navindra
full_name: Tajeshwar, Navindra
last_name: Tajeshwar
- first_name: Bruno
full_name: Catarino, Bruno
last_name: Catarino
- first_name: Alexander
full_name: Hetherington, Alexander
last_name: Hetherington
- first_name: Assia
full_name: Saltykova, Assia
last_name: Saltykova
- first_name: Clemence
full_name: Bonnot, Clemence
last_name: Bonnot
- first_name: Holger
full_name: Breuninger, Holger
last_name: Breuninger
- first_name: Aikaterini
full_name: Symeonidi, Aikaterini
last_name: Symeonidi
- first_name: Guru
full_name: Radhakrishnan, Guru
last_name: Radhakrishnan
- first_name: Filip
full_name: Van Nieuwerburgh, Filip
last_name: Van Nieuwerburgh
- first_name: Dieter
full_name: Deforce, Dieter
last_name: Deforce
- first_name: Caren
full_name: Chang, Caren
last_name: Chang
- first_name: Kenneth
full_name: Karol, Kenneth
last_name: Karol
- first_name: Rainer
full_name: Hedrich, Rainer
last_name: Hedrich
- first_name: Peter
full_name: Ulvskov, Peter
last_name: Ulvskov
- first_name: Gernot
full_name: Glöckner, Gernot
last_name: Glöckner
- first_name: Charles
full_name: Delwiche, Charles
last_name: Delwiche
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Yves
full_name: Van De Peer, Yves
last_name: Van De Peer
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Mary
full_name: Beilby, Mary
last_name: Beilby
- first_name: Liam
full_name: Dolan, Liam
last_name: Dolan
- first_name: Yuji
full_name: Kohara, Yuji
last_name: Kohara
- first_name: Sumio
full_name: Sugano, Sumio
last_name: Sugano
- first_name: Asao
full_name: Fujiyama, Asao
last_name: Fujiyama
- first_name: Pierre Marc
full_name: Delaux, Pierre Marc
last_name: Delaux
- first_name: Marcel
full_name: Quint, Marcel
last_name: Quint
- first_name: Gunter
full_name: Theissen, Gunter
last_name: Theissen
- first_name: Martin
full_name: Hagemann, Martin
last_name: Hagemann
- first_name: Jesper
full_name: Harholt, Jesper
last_name: Harholt
- first_name: Christophe
full_name: Dunand, Christophe
last_name: Dunand
- first_name: Sabine
full_name: Zachgo, Sabine
last_name: Zachgo
- first_name: Jane
full_name: Langdale, Jane
last_name: Langdale
- first_name: Florian
full_name: Maumus, Florian
last_name: Maumus
- first_name: Dominique
full_name: Van Der Straeten, Dominique
last_name: Van Der Straeten
- first_name: Sven B
full_name: Gould, Sven B
last_name: Gould
- first_name: Stefan
full_name: Rensing, Stefan
last_name: Rensing
citation:
ama: 'Nishiyama T, Sakayama H, De Vries J, et al. The Chara genome: Secondary complexity
and implications for plant terrestrialization. Cell. 2018;174(2):448-464.e24.
doi:10.1016/j.cell.2018.06.033'
apa: 'Nishiyama, T., Sakayama, H., De Vries, J., Buschmann, H., Saint Marcoux, D.,
Ullrich, K., … Rensing, S. (2018). The Chara genome: Secondary complexity and
implications for plant terrestrialization. Cell. Cell Press. https://doi.org/10.1016/j.cell.2018.06.033'
chicago: 'Nishiyama, Tomoaki, Hidetoshi Sakayama, Jan De Vries, Henrik Buschmann,
Denis Saint Marcoux, Kristian Ullrich, Fabian Haas, et al. “The Chara Genome:
Secondary Complexity and Implications for Plant Terrestrialization.” Cell.
Cell Press, 2018. https://doi.org/10.1016/j.cell.2018.06.033.'
ieee: 'T. Nishiyama et al., “The Chara genome: Secondary complexity and implications
for plant terrestrialization,” Cell, vol. 174, no. 2. Cell Press, p. 448–464.e24,
2018.'
ista: 'Nishiyama T, Sakayama H, De Vries J, Buschmann H, Saint Marcoux D, Ullrich
K, Haas F, Vanderstraeten L, Becker D, Lang D, Vosolsobě S, Rombauts S, Wilhelmsson
P, Janitza P, Kern R, Heyl A, Rümpler F, Calderón Villalobos L, Clay J, Skokan
R, Toyoda A, Suzuki Y, Kagoshima H, Schijlen E, Tajeshwar N, Catarino B, Hetherington
A, Saltykova A, Bonnot C, Breuninger H, Symeonidi A, Radhakrishnan G, Van Nieuwerburgh
F, Deforce D, Chang C, Karol K, Hedrich R, Ulvskov P, Glöckner G, Delwiche C,
Petrášek J, Van De Peer Y, Friml J, Beilby M, Dolan L, Kohara Y, Sugano S, Fujiyama
A, Delaux PM, Quint M, Theissen G, Hagemann M, Harholt J, Dunand C, Zachgo S,
Langdale J, Maumus F, Van Der Straeten D, Gould SB, Rensing S. 2018. The Chara
genome: Secondary complexity and implications for plant terrestrialization. Cell.
174(2), 448–464.e24.'
mla: 'Nishiyama, Tomoaki, et al. “The Chara Genome: Secondary Complexity and Implications
for Plant Terrestrialization.” Cell, vol. 174, no. 2, Cell Press, 2018,
p. 448–464.e24, doi:10.1016/j.cell.2018.06.033.'
short: T. Nishiyama, H. Sakayama, J. De Vries, H. Buschmann, D. Saint Marcoux, K.
Ullrich, F. Haas, L. Vanderstraeten, D. Becker, D. Lang, S. Vosolsobě, S. Rombauts,
P. Wilhelmsson, P. Janitza, R. Kern, A. Heyl, F. Rümpler, L. Calderón Villalobos,
J. Clay, R. Skokan, A. Toyoda, Y. Suzuki, H. Kagoshima, E. Schijlen, N. Tajeshwar,
B. Catarino, A. Hetherington, A. Saltykova, C. Bonnot, H. Breuninger, A. Symeonidi,
G. Radhakrishnan, F. Van Nieuwerburgh, D. Deforce, C. Chang, K. Karol, R. Hedrich,
P. Ulvskov, G. Glöckner, C. Delwiche, J. Petrášek, Y. Van De Peer, J. Friml, M.
Beilby, L. Dolan, Y. Kohara, S. Sugano, A. Fujiyama, P.M. Delaux, M. Quint, G.
Theissen, M. Hagemann, J. Harholt, C. Dunand, S. Zachgo, J. Langdale, F. Maumus,
D. Van Der Straeten, S.B. Gould, S. Rensing, Cell 174 (2018) 448–464.e24.
date_created: 2018-12-11T11:44:53Z
date_published: 2018-07-12T00:00:00Z
date_updated: 2023-09-19T10:02:47Z
day: '12'
department:
- _id: JiFr
doi: 10.1016/j.cell.2018.06.033
ec_funded: 1
external_id:
isi:
- '000438482800019'
pmid:
- '30007417'
intvolume: ' 174'
isi: 1
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pubmed/30007417
month: '07'
oa: 1
oa_version: Published Version
page: 448 - 464.e24
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Cell
publication_status: published
publisher: Cell Press
publist_id: '7774'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'The Chara genome: Secondary complexity and implications for plant terrestrialization'
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 174
year: '2018'
...
---
_id: '147'
abstract:
- lang: eng
text: The trafficking of subcellular cargos in eukaryotic cells crucially depends
on vesicle budding, a process mediated by ARF-GEFs (ADP-ribosylation factor guanine
nucleotide exchange factors). In plants, ARF-GEFs play essential roles in endocytosis,
vacuolar trafficking, recycling, secretion, and polar trafficking. Moreover, they
are important for plant development, mainly through controlling the polar subcellular
localization of PIN-FORMED (PIN) transporters of the plant hormone auxin. Here,
using a chemical genetics screen in Arabidopsis thaliana, we identified Endosidin
4 (ES4), an inhibitor of eukaryotic ARF-GEFs. ES4 acts similarly to and synergistically
with the established ARF-GEF inhibitor Brefeldin A and has broad effects on intracellular
trafficking, including endocytosis, exocytosis, and vacuolar targeting. Additionally,
Arabidopsis and yeast (Sacharomyces cerevisiae) mutants defective in ARF-GEF show
altered sensitivity to ES4. ES4 interferes with the activation-based membrane
association of the ARF1 GTPases, but not of their mutant variants that are activated
independently of ARF-GEF activity. Biochemical approaches and docking simulations
confirmed that ES4 specifically targets the SEC7 domain-containing ARF-GEFs. These
observations collectively identify ES4 as a chemical tool enabling the study of
ARF-GEF-mediated processes, including ARF-GEF-mediated plant development.
acknowledgement: We thank Gerd Jürgens, Sandra Richter, and Sheng Yang He for providing
antibodies; Maciek Adamowski, Fernando Aniento, Sebastian Bednarek, Nico Callewaert,
Matyás Fendrych, Elena Feraru, and Mugurel I. Feraru for helpful suggestions; Siamsa
Doyle for critical reading of the manuscript and helpful comments and suggestions;
and Stephanie Smith and Martine De Cock for help in editing and language corrections.
We acknowledge the core facility Cellular Imaging of CEITEC supported by the Czech-BioImaging
large RI project (LM2015062 funded by MEYS CR) for their support with obtaining
scientific data presented in this article. Plant Sciences Core Facility of CEITEC
Masaryk University is gratefully acknowledged for obtaining part of the scientific
data presented in this article. We acknowledge support from the Fondation pour la
Recherche Médicale and from the Institut National du Cancer (J.C.). The research
leading to these results was funded by the European Research Council under the European
Union's 7th Framework Program (FP7/2007-2013)/ERC grant agreement numbers 282300
and 742985 and the Czech Science Foundation GAČR (GA18-26981S; J.F.); Ministry of
Education, Youth, and Sports/MEYS of the Czech Republic under the Project CEITEC
2020 (LQ1601; T.N.); the China Science Council for a predoctoral fellowship (Q.L.);
a joint research project within the framework of cooperation between the Research
Foundation-Flanders and the Bulgarian Academy of Sciences (VS.025.13N; K.M. and
E.R.); Vetenskapsrådet and Vinnova (Verket för Innovationssystem; S.R.), Knut och
Alice Wallenbergs Stiftelse via “Shapesystem” Grant 2012.0050 (S.R.), Kempe stiftelserna
(P.G.), Tryggers CTS410 (P.G.).
article_processing_charge: No
article_type: original
author:
- first_name: Urszula
full_name: Kania, Urszula
id: 4AE5C486-F248-11E8-B48F-1D18A9856A87
last_name: Kania
- first_name: Tomasz
full_name: Nodzyński, Tomasz
last_name: Nodzyński
- first_name: Qing
full_name: Lu, Qing
last_name: Lu
- first_name: Glenn R
full_name: Hicks, Glenn R
last_name: Hicks
- first_name: Wim
full_name: Nerinckx, Wim
last_name: Nerinckx
- first_name: Kiril
full_name: Mishev, Kiril
last_name: Mishev
- first_name: Francois
full_name: Peurois, Francois
last_name: Peurois
- first_name: Jacqueline
full_name: Cherfils, Jacqueline
last_name: Cherfils
- first_name: Rycke Riet Maria
full_name: De, Rycke Riet Maria
last_name: De
- first_name: Peter
full_name: Grones, Peter
id: 399876EC-F248-11E8-B48F-1D18A9856A87
last_name: Grones
- first_name: Stéphanie
full_name: Robert, Stéphanie
last_name: Robert
- first_name: Eugenia
full_name: Russinova, Eugenia
last_name: Russinova
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Kania U, Nodzyński T, Lu Q, et al. The inhibitor Endosidin 4 targets SEC7 domain-type
ARF GTPase exchange factors and interferes with sub cellular trafficking in eukaryotes.
The Plant Cell. 2018;30(10):2553-2572. doi:10.1105/tpc.18.00127
apa: Kania, U., Nodzyński, T., Lu, Q., Hicks, G. R., Nerinckx, W., Mishev, K., …
Friml, J. (2018). The inhibitor Endosidin 4 targets SEC7 domain-type ARF GTPase
exchange factors and interferes with sub cellular trafficking in eukaryotes. The
Plant Cell. Oxford University Press. https://doi.org/10.1105/tpc.18.00127
chicago: Kania, Urszula, Tomasz Nodzyński, Qing Lu, Glenn R Hicks, Wim Nerinckx,
Kiril Mishev, Francois Peurois, et al. “The Inhibitor Endosidin 4 Targets SEC7
Domain-Type ARF GTPase Exchange Factors and Interferes with Sub Cellular Trafficking
in Eukaryotes.” The Plant Cell. Oxford University Press, 2018. https://doi.org/10.1105/tpc.18.00127.
ieee: U. Kania et al., “The inhibitor Endosidin 4 targets SEC7 domain-type
ARF GTPase exchange factors and interferes with sub cellular trafficking in eukaryotes,”
The Plant Cell, vol. 30, no. 10. Oxford University Press, pp. 2553–2572,
2018.
ista: Kania U, Nodzyński T, Lu Q, Hicks GR, Nerinckx W, Mishev K, Peurois F, Cherfils
J, De RRM, Grones P, Robert S, Russinova E, Friml J. 2018. The inhibitor Endosidin
4 targets SEC7 domain-type ARF GTPase exchange factors and interferes with sub
cellular trafficking in eukaryotes. The Plant Cell. 30(10), 2553–2572.
mla: Kania, Urszula, et al. “The Inhibitor Endosidin 4 Targets SEC7 Domain-Type
ARF GTPase Exchange Factors and Interferes with Sub Cellular Trafficking in Eukaryotes.”
The Plant Cell, vol. 30, no. 10, Oxford University Press, 2018, pp. 2553–72,
doi:10.1105/tpc.18.00127.
short: U. Kania, T. Nodzyński, Q. Lu, G.R. Hicks, W. Nerinckx, K. Mishev, F. Peurois,
J. Cherfils, R.R.M. De, P. Grones, S. Robert, E. Russinova, J. Friml, The Plant
Cell 30 (2018) 2553–2572.
date_created: 2018-12-11T11:44:52Z
date_published: 2018-11-12T00:00:00Z
date_updated: 2023-09-19T10:09:12Z
day: '12'
department:
- _id: JiFr
doi: 10.1105/tpc.18.00127
ec_funded: 1
external_id:
isi:
- '000450000500023'
pmid:
- '30018156'
intvolume: ' 30'
isi: 1
issue: '10'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1105/tpc.18.00127
month: '11'
oa: 1
oa_version: Published Version
page: 2553 - 2572
pmid: 1
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: The Plant Cell
publication_identifier:
issn:
- 1040-4651
publication_status: published
publisher: Oxford University Press
publist_id: '7776'
quality_controlled: '1'
scopus_import: '1'
status: public
title: The inhibitor Endosidin 4 targets SEC7 domain-type ARF GTPase exchange factors
and interferes with sub cellular trafficking in eukaryotes
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 30
year: '2018'
...
---
_id: '146'
abstract:
- lang: eng
text: The root cap protects the stem cell niche of angiosperm roots from damage.
In Arabidopsis, lateral root cap (LRC) cells covering the meristematic zone are
regularly lost through programmed cell death, while the outermost layer of the
root cap covering the tip is repeatedly sloughed. Efficient coordination with
stem cells producing new layers is needed to maintain a constant size of the cap.
We present a signalling pair, the peptide IDA-LIKE1 (IDL1) and its receptor HAESA-LIKE2
(HSL2), mediating such communication. Live imaging over several days characterized
this process from initial fractures in LRC cell files to full separation of a
layer. Enhanced expression of IDL1 in the separating root cap layers resulted
in increased frequency of sloughing, balanced with generation of new layers in
a HSL2-dependent manner. Transcriptome analyses linked IDL1-HSL2 signalling to
the transcription factors BEARSKIN1/2 and genes associated with programmed cell
death. Mutations in either IDL1 or HSL2 slowed down cell division, maturation
and separation. Thus, IDL1-HSL2 signalling potentiates dynamic regulation of the
homeostatic balance between stem cell division and sloughing activity.
article_processing_charge: No
article_type: original
author:
- first_name: Chun Lin
full_name: Shi, Chun Lin
last_name: Shi
- first_name: Daniel
full_name: Von Wangenheim, Daniel
id: 49E91952-F248-11E8-B48F-1D18A9856A87
last_name: Von Wangenheim
orcid: 0000-0002-6862-1247
- first_name: Ullrich
full_name: Herrmann, Ullrich
last_name: Herrmann
- first_name: Mari
full_name: Wildhagen, Mari
last_name: Wildhagen
- first_name: Ivan
full_name: Kulik, Ivan
id: F0AB3FCE-02D1-11E9-BD0E-99399A5D3DEB
last_name: Kulik
- first_name: Andreas
full_name: Kopf, Andreas
last_name: Kopf
- first_name: Takashi
full_name: Ishida, Takashi
last_name: Ishida
- first_name: Vilde
full_name: Olsson, Vilde
last_name: Olsson
- first_name: Mari Kristine
full_name: Anker, Mari Kristine
last_name: Anker
- first_name: Markus
full_name: Albert, Markus
last_name: Albert
- first_name: Melinka A
full_name: Butenko, Melinka A
last_name: Butenko
- first_name: Georg
full_name: Felix, Georg
last_name: Felix
- first_name: Shinichiro
full_name: Sawa, Shinichiro
last_name: Sawa
- first_name: Manfred
full_name: Claassen, Manfred
last_name: Claassen
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Reidunn B
full_name: Aalen, Reidunn B
last_name: Aalen
citation:
ama: Shi CL, von Wangenheim D, Herrmann U, et al. The dynamics of root cap sloughing
in Arabidopsis is regulated by peptide signalling. Nature Plants. 2018;4(8):596-604.
doi:10.1038/s41477-018-0212-z
apa: Shi, C. L., von Wangenheim, D., Herrmann, U., Wildhagen, M., Kulik, I., Kopf,
A., … Aalen, R. B. (2018). The dynamics of root cap sloughing in Arabidopsis is
regulated by peptide signalling. Nature Plants. Nature Publishing Group.
https://doi.org/10.1038/s41477-018-0212-z
chicago: Shi, Chun Lin, Daniel von Wangenheim, Ullrich Herrmann, Mari Wildhagen,
Ivan Kulik, Andreas Kopf, Takashi Ishida, et al. “The Dynamics of Root Cap Sloughing
in Arabidopsis Is Regulated by Peptide Signalling.” Nature Plants. Nature
Publishing Group, 2018. https://doi.org/10.1038/s41477-018-0212-z.
ieee: C. L. Shi et al., “The dynamics of root cap sloughing in Arabidopsis
is regulated by peptide signalling,” Nature Plants, vol. 4, no. 8. Nature
Publishing Group, pp. 596–604, 2018.
ista: Shi CL, von Wangenheim D, Herrmann U, Wildhagen M, Kulik I, Kopf A, Ishida
T, Olsson V, Anker MK, Albert M, Butenko MA, Felix G, Sawa S, Claassen M, Friml
J, Aalen RB. 2018. The dynamics of root cap sloughing in Arabidopsis is regulated
by peptide signalling. Nature Plants. 4(8), 596–604.
mla: Shi, Chun Lin, et al. “The Dynamics of Root Cap Sloughing in Arabidopsis Is
Regulated by Peptide Signalling.” Nature Plants, vol. 4, no. 8, Nature
Publishing Group, 2018, pp. 596–604, doi:10.1038/s41477-018-0212-z.
short: C.L. Shi, D. von Wangenheim, U. Herrmann, M. Wildhagen, I. Kulik, A. Kopf,
T. Ishida, V. Olsson, M.K. Anker, M. Albert, M.A. Butenko, G. Felix, S. Sawa,
M. Claassen, J. Friml, R.B. Aalen, Nature Plants 4 (2018) 596–604.
date_created: 2018-12-11T11:44:52Z
date_published: 2018-07-30T00:00:00Z
date_updated: 2023-09-19T10:08:45Z
day: '30'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41477-018-0212-z
external_id:
isi:
- '000443861300016'
pmid:
- '30061750'
file:
- access_level: open_access
checksum: da33101c76ee1b2dc5ab28fd2ccba9d0
content_type: application/pdf
creator: dernst
date_created: 2019-11-18T16:24:07Z
date_updated: 2020-07-14T12:44:56Z
file_id: '7043'
file_name: 2018_NaturePlants_Shi.pdf
file_size: 226829
relation: main_file
file_date_updated: 2020-07-14T12:44:56Z
has_accepted_license: '1'
intvolume: ' 4'
isi: 1
issue: '8'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Submitted Version
page: 596 - 604
pmid: 1
publication: Nature Plants
publication_status: published
publisher: Nature Publishing Group
publist_id: '7777'
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/new-process-in-root-development-discovered/
scopus_import: '1'
status: public
title: The dynamics of root cap sloughing in Arabidopsis is regulated by peptide signalling
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 4
year: '2018'
...
---
_id: '10881'
abstract:
- lang: eng
text: Strigolactones (SLs) are a relatively recent addition to the list of plant
hormones that control different aspects of plant development. SL signalling is
perceived by an α/β hydrolase, DWARF 14 (D14). A close homolog of D14, KARRIKIN
INSENSTIVE2 (KAI2), is involved in perception of an uncharacterized molecule called
karrikin (KAR). Recent studies in Arabidopsis identified the SUPPRESSOR OF MAX2
1 (SMAX1) and SMAX1-LIKE 7 (SMXL7) to be potential SCF–MAX2 complex-mediated proteasome
targets of KAI2 and D14, respectively. Genetic studies on SMXL7 and SMAX1 demonstrated
distinct developmental roles for each, but very little is known about these repressors
in terms of their sequence features. In this study, we performed an extensive
comparative analysis of SMXLs and determined their phylogenetic and evolutionary
history in the plant lineage. Our results show that SMXL family members can be
sub-divided into four distinct phylogenetic clades/classes, with an ancient SMAX1.
Further, we identified the clade-specific motifs that have evolved and that might
act as determinants of SL-KAR signalling specificity. These specificities resulted
from functional diversities among the clades. Our results suggest that a gradual
co-evolution of SMXL members with their upstream receptors D14/KAI2 provided an
increased specificity to both the SL perception and response in land plants.
acknowledgement: "This project received funding from the European Union’s Horizon
2020 research and innovation programme under the Marie Skłodowska-Curie Actions
and it is co-financed by the South Moravian Region under grant agreement No. 665860
(SS). Access to computing and storage facilities owned by parties and projects contributing
to the national grid infrastructure, MetaCentrum, provided under the program ‘Projects
of Large Infrastructure for Research, Development, and Innovations’ (LM2010005)
was greatly appreciated (RSV). The project was funded by The Ministry of Education,
Youth and Sports/MES of the Czech Republic under the project CEITEC 2020 (LQ1601)
(TN, TRM). JF was supported by the European Research Council (project ERC-2011-StG
20101109-PSDP) and the Czech Science Foundation GAČR (GA13-40637S). We thank Dr
Kamel Chibani for active discussions on the evolutionary analysis and Nandan Mysore
Vardarajan for his critical comments on the manuscript. This article reflects\r\nonly
the authors’ views, and the EU is not responsible for any use that may be made of
the information it contains. "
article_processing_charge: No
article_type: original
author:
- first_name: Taraka Ramji
full_name: Moturu, Taraka Ramji
last_name: Moturu
- first_name: Sravankumar
full_name: Thula, Sravankumar
last_name: Thula
- first_name: Ravi Kumar
full_name: Singh, Ravi Kumar
last_name: Singh
- first_name: Tomasz
full_name: Nodzyński, Tomasz
last_name: Nodzyński
- first_name: Radka Svobodová
full_name: Vařeková, Radka Svobodová
last_name: Vařeková
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Sibu
full_name: Simon, Sibu
last_name: Simon
citation:
ama: Moturu TR, Thula S, Singh RK, et al. Molecular evolution and diversification
of the SMXL gene family. Journal of Experimental Botany. 2018;69(9):2367-2378.
doi:10.1093/jxb/ery097
apa: Moturu, T. R., Thula, S., Singh, R. K., Nodzyński, T., Vařeková, R. S., Friml,
J., & Simon, S. (2018). Molecular evolution and diversification of the SMXL
gene family. Journal of Experimental Botany. Oxford University Press. https://doi.org/10.1093/jxb/ery097
chicago: Moturu, Taraka Ramji, Sravankumar Thula, Ravi Kumar Singh, Tomasz Nodzyński,
Radka Svobodová Vařeková, Jiří Friml, and Sibu Simon. “Molecular Evolution and
Diversification of the SMXL Gene Family.” Journal of Experimental Botany.
Oxford University Press, 2018. https://doi.org/10.1093/jxb/ery097.
ieee: T. R. Moturu et al., “Molecular evolution and diversification of the
SMXL gene family,” Journal of Experimental Botany, vol. 69, no. 9. Oxford
University Press, pp. 2367–2378, 2018.
ista: Moturu TR, Thula S, Singh RK, Nodzyński T, Vařeková RS, Friml J, Simon S.
2018. Molecular evolution and diversification of the SMXL gene family. Journal
of Experimental Botany. 69(9), 2367–2378.
mla: Moturu, Taraka Ramji, et al. “Molecular Evolution and Diversification of the
SMXL Gene Family.” Journal of Experimental Botany, vol. 69, no. 9, Oxford
University Press, 2018, pp. 2367–78, doi:10.1093/jxb/ery097.
short: T.R. Moturu, S. Thula, R.K. Singh, T. Nodzyński, R.S. Vařeková, J. Friml,
S. Simon, Journal of Experimental Botany 69 (2018) 2367–2378.
date_created: 2022-03-18T12:43:22Z
date_published: 2018-04-13T00:00:00Z
date_updated: 2023-09-19T15:10:43Z
day: '13'
department:
- _id: JiFr
doi: 10.1093/jxb/ery097
ec_funded: 1
external_id:
isi:
- '000430727000016'
pmid:
- '29538714'
intvolume: ' 69'
isi: 1
issue: '9'
keyword:
- Plant Science
- Physiology
language:
- iso: eng
month: '04'
oa_version: None
page: 2367-2378
pmid: 1
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Journal of Experimental Botany
publication_identifier:
eissn:
- 1460-2431
issn:
- 0022-0957
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Molecular evolution and diversification of the SMXL gene family
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 69
year: '2018'
...
---
_id: '913'
abstract:
- lang: eng
text: Coordinated cell polarization in developing tissues is a recurrent theme in
multicellular organisms. In plants, a directional distribution of the plant hormone
auxin is at the core of many developmental programs. A feedback regulation of
auxin on the polarized localization of PIN auxin transporters in individual cells
has been proposed as a self-organizing mechanism for coordinated tissue polarization,
but the molecular mechanisms linking auxin signalling to PIN-dependent auxin transport
remain unknown. We performed a microarray-based approach to find regulators of
the auxin-induced PIN relocation in the Arabidopsis thaliana root. We identified
a subset of a family of phosphatidylinositol transfer proteins (PITP), the PATELLINs
(PATL). Here, we show that PATLs are expressed in partially overlapping cells
types in different tissues going through mitosis or initiating differentiation
programs. PATLs are plasma membrane-associated proteins accumulated in Arabidopsis
embryos, primary roots, lateral root primordia, and developing stomata. Higher
order patl mutants display reduced PIN1 repolarization in response to auxin, shorter
root apical meristem, and drastic defects in embryo and seedling development.
This suggests PATLs redundantly play a crucial role in polarity and patterning
in Arabidopsis.
article_number: jcs.204198
article_processing_charge: No
author:
- first_name: Ricardo
full_name: Tejos, Ricardo
last_name: Tejos
- first_name: Cecilia
full_name: Rodríguez Furlán, Cecilia
last_name: Rodríguez Furlán
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Michael
full_name: Sauer, Michael
last_name: Sauer
- first_name: Lorena
full_name: Norambuena, Lorena
last_name: Norambuena
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Tejos R, Rodríguez Furlán C, Adamowski M, Sauer M, Norambuena L, Friml J. PATELLINS
are regulators of auxin mediated PIN1 relocation and plant development in Arabidopsis
thaliana. Journal of Cell Science. 2018;131(2). doi:10.1242/jcs.204198
apa: Tejos, R., Rodríguez Furlán, C., Adamowski, M., Sauer, M., Norambuena, L.,
& Friml, J. (2018). PATELLINS are regulators of auxin mediated PIN1 relocation
and plant development in Arabidopsis thaliana. Journal of Cell Science.
Company of Biologists. https://doi.org/10.1242/jcs.204198
chicago: Tejos, Ricardo, Cecilia Rodríguez Furlán, Maciek Adamowski, Michael Sauer,
Lorena Norambuena, and Jiří Friml. “PATELLINS Are Regulators of Auxin Mediated
PIN1 Relocation and Plant Development in Arabidopsis Thaliana.” Journal of
Cell Science. Company of Biologists, 2018. https://doi.org/10.1242/jcs.204198.
ieee: R. Tejos, C. Rodríguez Furlán, M. Adamowski, M. Sauer, L. Norambuena, and
J. Friml, “PATELLINS are regulators of auxin mediated PIN1 relocation and plant
development in Arabidopsis thaliana,” Journal of Cell Science, vol. 131,
no. 2. Company of Biologists, 2018.
ista: Tejos R, Rodríguez Furlán C, Adamowski M, Sauer M, Norambuena L, Friml J.
2018. PATELLINS are regulators of auxin mediated PIN1 relocation and plant development
in Arabidopsis thaliana. Journal of Cell Science. 131(2), jcs. 204198.
mla: Tejos, Ricardo, et al. “PATELLINS Are Regulators of Auxin Mediated PIN1 Relocation
and Plant Development in Arabidopsis Thaliana.” Journal of Cell Science,
vol. 131, no. 2, jcs. 204198, Company of Biologists, 2018, doi:10.1242/jcs.204198.
short: R. Tejos, C. Rodríguez Furlán, M. Adamowski, M. Sauer, L. Norambuena, J.
Friml, Journal of Cell Science 131 (2018).
date_created: 2018-12-11T11:49:10Z
date_published: 2018-01-29T00:00:00Z
date_updated: 2023-09-26T15:47:50Z
day: '29'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.1242/jcs.204198
ec_funded: 1
external_id:
isi:
- '000424842400019'
file:
- access_level: open_access
checksum: bf156c20a4f117b4b932370d54cbac8c
content_type: application/pdf
creator: dernst
date_created: 2019-04-12T08:46:32Z
date_updated: 2020-07-14T12:48:15Z
file_id: '6299'
file_name: 2017_adamowski_PATELLINS_are.pdf
file_size: 14925985
relation: main_file
file_date_updated: 2020-07-14T12:48:15Z
has_accepted_license: '1'
intvolume: ' 131'
isi: 1
issue: '2'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Journal of Cell Science
publication_identifier:
issn:
- '00219533'
publication_status: published
publisher: Company of Biologists
publist_id: '6530'
pubrep_id: '988'
quality_controlled: '1'
scopus_import: '1'
status: public
title: PATELLINS are regulators of auxin mediated PIN1 relocation and plant development
in Arabidopsis thaliana
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 131
year: '2018'
...
---
_id: '5673'
abstract:
- lang: eng
text: Cell polarity, manifested by the localization of proteins to distinct polar
plasma membrane domains, is a key prerequisite of multicellular life. In plants,
PIN auxin transporters are prominent polarity markers crucial for a plethora of
developmental processes. Cell polarity mechanisms in plants are distinct from
other eukaryotes and still largely elusive. In particular, how the cell polarities
are propagated and maintained following cell division remains unknown. Plant cytokinesis
is orchestrated by the cell plate—a transient centrifugally growing endomembrane
compartment ultimately forming the cross wall1. Trafficking of polar membrane
proteins is typically redirected to the cell plate, and these will consequently
have opposite polarity in at least one of the daughter cells2–5. Here, we provide
mechanistic insights into post-cytokinetic re-establishment of cell polarity as
manifested by the apical, polar localization of PIN2. We show that the apical
domain is defined in a cell-intrinsic manner and that re-establishment of PIN2
localization to this domain requires de novo protein secretion and endocytosis,
but not basal-to-apical transcytosis. Furthermore, we identify a PINOID-related
kinase WAG1, which phosphorylates PIN2 in vitro6 and is transcriptionally upregulated
specifically in dividing cells, as a crucial regulator of post-cytokinetic PIN2
polarity re-establishment.
article_processing_charge: No
author:
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Glanc M, Fendrych M, Friml J. Mechanistic framework for cell-intrinsic re-establishment
of PIN2 polarity after cell division. Nature Plants. 2018;4(12):1082-1088.
doi:10.1038/s41477-018-0318-3
apa: Glanc, M., Fendrych, M., & Friml, J. (2018). Mechanistic framework for
cell-intrinsic re-establishment of PIN2 polarity after cell division. Nature
Plants. Nature Research. https://doi.org/10.1038/s41477-018-0318-3
chicago: Glanc, Matous, Matyas Fendrych, and Jiří Friml. “Mechanistic Framework
for Cell-Intrinsic Re-Establishment of PIN2 Polarity after Cell Division.” Nature
Plants. Nature Research, 2018. https://doi.org/10.1038/s41477-018-0318-3.
ieee: M. Glanc, M. Fendrych, and J. Friml, “Mechanistic framework for cell-intrinsic
re-establishment of PIN2 polarity after cell division,” Nature Plants,
vol. 4, no. 12. Nature Research, pp. 1082–1088, 2018.
ista: Glanc M, Fendrych M, Friml J. 2018. Mechanistic framework for cell-intrinsic
re-establishment of PIN2 polarity after cell division. Nature Plants. 4(12), 1082–1088.
mla: Glanc, Matous, et al. “Mechanistic Framework for Cell-Intrinsic Re-Establishment
of PIN2 Polarity after Cell Division.” Nature Plants, vol. 4, no. 12, Nature
Research, 2018, pp. 1082–88, doi:10.1038/s41477-018-0318-3.
short: M. Glanc, M. Fendrych, J. Friml, Nature Plants 4 (2018) 1082–1088.
date_created: 2018-12-16T22:59:18Z
date_published: 2018-12-03T00:00:00Z
date_updated: 2023-10-17T12:19:28Z
day: '03'
department:
- _id: JiFr
doi: 10.1038/s41477-018-0318-3
ec_funded: 1
external_id:
isi:
- '000454576600017'
pmid:
- '30518833'
intvolume: ' 4'
isi: 1
issue: '12'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pubmed/30518833
month: '12'
oa: 1
oa_version: Submitted Version
page: 1082-1088
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Nature Plants
publication_identifier:
issn:
- 2055-0278
publication_status: published
publisher: Nature Research
quality_controlled: '1'
scopus_import: '1'
status: public
title: Mechanistic framework for cell-intrinsic re-establishment of PIN2 polarity
after cell division
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 4
year: '2018'
...
---
_id: '412'
abstract:
- lang: eng
text: Clathrin-mediated endocytosis (CME) is a cellular trafficking process in which
cargoes and lipids are internalized from the plasma membrane into vesicles coated
with clathrin and adaptor proteins. CME is essential for many developmental and
physiological processes in plants, but its underlying mechanism is not well characterised
compared to that in yeast and animal systems. Here, we searched for new factors
involved in CME in Arabidopsis thaliana by performing Tandem Affinity Purification
of proteins that interact with clathrin light chain, a principal component of
the clathrin coat. Among the confirmed interactors, we found two putative homologues
of the clathrin-coat uncoating factor auxilin previously described in non-plant
systems. Overexpression of AUXILIN-LIKE1 and AUXILIN-LIKE2 in A. thaliana caused
an arrest of seedling growth and development. This was concomitant with inhibited
endocytosis due to blocking of clathrin recruitment after the initial step of
adaptor protein binding to the plasma membrane. By contrast, auxilin-like(1/2)
loss-of-function lines did not present endocytosis-related developmental or cellular
phenotypes under normal growth conditions. This work contributes to the on-going
characterization of the endocytotic machinery in plants and provides a robust
tool for conditionally and specifically interfering with CME in A. thaliana.
acknowledgement: We thank James Matthew Watson, Monika Borowska, and Peggy Stolt-Bergner
at ProTech Facility of the Vienna Biocenter Core Facilities for the CRISPR/CAS9
construct; Anna Müller for assistance with molecular cloning; Sebastian Bednarek,
Liwen Jiang, and Daniël Van Damme for sharing published material; Matyáš Fendrych,
Daniël Van Damme, and Lindy Abas for valuable discussions; and Martine De Cock for
help with correcting the manuscript. This work was supported by the European Research
Council under the European Union Seventh Framework Programme (FP7/2007-2013)/ERC
Grant 282300 and by the Ministry of Education of the Czech Republic/MŠMT project
NPUI-LO1417.
article_processing_charge: No
article_type: original
author:
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Madhumitha
full_name: Narasimhan, Madhumitha
id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
last_name: Narasimhan
orcid: 0000-0002-8600-0671
- first_name: Urszula
full_name: Kania, Urszula
id: 4AE5C486-F248-11E8-B48F-1D18A9856A87
last_name: Kania
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: Geert
full_name: De Jaeger, Geert
last_name: De Jaeger
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Adamowski M, Narasimhan M, Kania U, Glanc M, De Jaeger G, Friml J. A functional
study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors in Arabidopsis.
The Plant Cell. 2018;30(3):700-716. doi:10.1105/tpc.17.00785
apa: Adamowski, M., Narasimhan, M., Kania, U., Glanc, M., De Jaeger, G., & Friml,
J. (2018). A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating
factors in Arabidopsis. The Plant Cell. American Society of Plant Biologists.
https://doi.org/10.1105/tpc.17.00785
chicago: Adamowski, Maciek, Madhumitha Narasimhan, Urszula Kania, Matous Glanc,
Geert De Jaeger, and Jiří Friml. “A Functional Study of AUXILIN LIKE1 and 2 Two
Putative Clathrin Uncoating Factors in Arabidopsis.” The Plant Cell. American
Society of Plant Biologists, 2018. https://doi.org/10.1105/tpc.17.00785.
ieee: M. Adamowski, M. Narasimhan, U. Kania, M. Glanc, G. De Jaeger, and J. Friml,
“A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors
in Arabidopsis,” The Plant Cell, vol. 30, no. 3. American Society of Plant
Biologists, pp. 700–716, 2018.
ista: Adamowski M, Narasimhan M, Kania U, Glanc M, De Jaeger G, Friml J. 2018. A
functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors
in Arabidopsis. The Plant Cell. 30(3), 700–716.
mla: Adamowski, Maciek, et al. “A Functional Study of AUXILIN LIKE1 and 2 Two Putative
Clathrin Uncoating Factors in Arabidopsis.” The Plant Cell, vol. 30, no.
3, American Society of Plant Biologists, 2018, pp. 700–16, doi:10.1105/tpc.17.00785.
short: M. Adamowski, M. Narasimhan, U. Kania, M. Glanc, G. De Jaeger, J. Friml,
The Plant Cell 30 (2018) 700–716.
date_created: 2018-12-11T11:46:20Z
date_published: 2018-04-09T00:00:00Z
date_updated: 2024-03-18T23:30:06Z
day: '09'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1105/tpc.17.00785
ec_funded: 1
external_id:
isi:
- '000429441400018'
pmid:
- '29511054'
file:
- access_level: open_access
checksum: 4e165e653b67d3f0684697f21aace5a1
content_type: application/pdf
creator: dernst
date_created: 2022-05-23T09:12:38Z
date_updated: 2022-05-23T09:12:38Z
file_id: '11406'
file_name: 2018_PlantCell_Adamowski.pdf
file_size: 4407538
relation: main_file
success: 1
file_date_updated: 2022-05-23T09:12:38Z
has_accepted_license: '1'
intvolume: ' 30'
isi: 1
issue: '3'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
page: 700 - 716
pmid: 1
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: The Plant Cell
publication_identifier:
eissn:
- 1532-298X
issn:
- 1040-4651
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '7417'
quality_controlled: '1'
related_material:
record:
- id: '6269'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors
in Arabidopsis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 30
year: '2018'
...
---
_id: '449'
abstract:
- lang: eng
text: Auxin is unique among plant hormones due to its directional transport that
is mediated by the polarly distributed PIN auxin transporters at the plasma membrane.
The canalization hypothesis proposes that the auxin feedback on its polar flow
is a crucial, plant-specific mechanism mediating multiple self-organizing developmental
processes. Here, we used the auxin effect on the PIN polar localization in Arabidopsis
thaliana roots as a proxy for the auxin feedback on the PIN polarity during canalization.
We performed microarray experiments to find regulators of this process that act
downstream of auxin. We identified genes that were transcriptionally regulated
by auxin in an AXR3/IAA17- and ARF7/ARF19-dependent manner. Besides the known
components of the PIN polarity, such as PID and PIP5K kinases, a number of potential
new regulators were detected, among which the WRKY23 transcription factor, which
was characterized in more detail. Gain- and loss-of-function mutants confirmed
a role for WRKY23 in mediating the auxin effect on the PIN polarity. Accordingly,
processes requiring auxin-mediated PIN polarity rearrangements, such as vascular
tissue development during leaf venation, showed a higher WRKY23 expression and
required the WRKY23 activity. Our results provide initial insights into the auxin
transcriptional network acting upstream of PIN polarization and, potentially,
canalization-mediated plant development.
article_processing_charge: Yes
author:
- first_name: Tomas
full_name: Prat, Tomas
id: 3DA3BFEE-F248-11E8-B48F-1D18A9856A87
last_name: Prat
- first_name: Jakub
full_name: Hajny, Jakub
id: 4800CC20-F248-11E8-B48F-1D18A9856A87
last_name: Hajny
orcid: 0000-0003-2140-7195
- first_name: Wim
full_name: Grunewald, Wim
last_name: Grunewald
- first_name: Mina K
full_name: Vasileva, Mina K
id: 3407EB18-F248-11E8-B48F-1D18A9856A87
last_name: Vasileva
- first_name: Gergely
full_name: Molnar, Gergely
id: 34F1AF46-F248-11E8-B48F-1D18A9856A87
last_name: Molnar
- first_name: Ricardo
full_name: Tejos, Ricardo
last_name: Tejos
- first_name: Markus
full_name: Schmid, Markus
last_name: Schmid
- first_name: Michael
full_name: Sauer, Michael
last_name: Sauer
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Prat T, Hajny J, Grunewald W, et al. WRKY23 is a component of the transcriptional
network mediating auxin feedback on PIN polarity. PLoS Genetics. 2018;14(1).
doi:10.1371/journal.pgen.1007177
apa: Prat, T., Hajny, J., Grunewald, W., Vasileva, M. K., Molnar, G., Tejos, R.,
… Friml, J. (2018). WRKY23 is a component of the transcriptional network mediating
auxin feedback on PIN polarity. PLoS Genetics. Public Library of Science.
https://doi.org/10.1371/journal.pgen.1007177
chicago: Prat, Tomas, Jakub Hajny, Wim Grunewald, Mina K Vasileva, Gergely Molnar,
Ricardo Tejos, Markus Schmid, Michael Sauer, and Jiří Friml. “WRKY23 Is a Component
of the Transcriptional Network Mediating Auxin Feedback on PIN Polarity.” PLoS
Genetics. Public Library of Science, 2018. https://doi.org/10.1371/journal.pgen.1007177.
ieee: T. Prat et al., “WRKY23 is a component of the transcriptional network
mediating auxin feedback on PIN polarity,” PLoS Genetics, vol. 14, no.
1. Public Library of Science, 2018.
ista: Prat T, Hajny J, Grunewald W, Vasileva MK, Molnar G, Tejos R, Schmid M, Sauer
M, Friml J. 2018. WRKY23 is a component of the transcriptional network mediating
auxin feedback on PIN polarity. PLoS Genetics. 14(1).
mla: Prat, Tomas, et al. “WRKY23 Is a Component of the Transcriptional Network Mediating
Auxin Feedback on PIN Polarity.” PLoS Genetics, vol. 14, no. 1, Public
Library of Science, 2018, doi:10.1371/journal.pgen.1007177.
short: T. Prat, J. Hajny, W. Grunewald, M.K. Vasileva, G. Molnar, R. Tejos, M. Schmid,
M. Sauer, J. Friml, PLoS Genetics 14 (2018).
date_created: 2018-12-11T11:46:32Z
date_published: 2018-01-29T00:00:00Z
date_updated: 2024-03-18T23:30:39Z
day: '29'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.1371/journal.pgen.1007177
ec_funded: 1
external_id:
isi:
- '000423718600034'
file:
- access_level: open_access
checksum: 0276d66788ec076f4924164a39e6a712
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:10:52Z
date_updated: 2020-07-14T12:46:30Z
file_id: '4843'
file_name: IST-2018-967-v1+1_journal.pgen.1007177.pdf
file_size: 24709062
relation: main_file
file_date_updated: 2020-07-14T12:46:30Z
has_accepted_license: '1'
intvolume: ' 14'
isi: 1
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: PLoS Genetics
publication_status: published
publisher: Public Library of Science
publist_id: '7373'
pubrep_id: '967'
quality_controlled: '1'
related_material:
record:
- id: '1127'
relation: dissertation_contains
status: public
- id: '7172'
relation: dissertation_contains
status: public
- id: '8822'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: WRKY23 is a component of the transcriptional network mediating auxin feedback
on PIN polarity
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 14
year: '2018'
...
---
_id: '191'
abstract:
- lang: eng
text: Intercellular distribution of the plant hormone auxin largely depends on the
polar subcellular distribution of the plasma membrane PIN-FORMED (PIN) auxin transporters.
PIN polarity switches in response to different developmental and environmental
signals have been shown to redirect auxin fluxes mediating certain developmental
responses. PIN phosphorylation at different sites and by different kinases is
crucial for PIN function. Here we investigate the role of PIN phosphorylation
during gravitropic response. Loss- and gain-of-function mutants in PINOID and
related kinases but not in D6PK kinase as well as mutations mimicking constitutive
dephosphorylated or phosphorylated status of two clusters of predicted phosphorylation
sites partially disrupted PIN3 phosphorylation and caused defects in gravitropic
bending in roots and hypocotyls. In particular, they impacted PIN3 polarity rearrangements
in response to gravity and during feed-back regulation by auxin itself. Thus PIN
phosphorylation, besides regulating transport activity and apical-basal targeting,
is also important for the rapid polarity switches in response to environmental
and endogenous signals.
article_number: '10279'
article_processing_charge: No
author:
- first_name: Peter
full_name: Grones, Peter
id: 399876EC-F248-11E8-B48F-1D18A9856A87
last_name: Grones
- first_name: Melinda F
full_name: Abas, Melinda F
id: 3CFB3B1C-F248-11E8-B48F-1D18A9856A87
last_name: Abas
- first_name: Jakub
full_name: Hajny, Jakub
id: 4800CC20-F248-11E8-B48F-1D18A9856A87
last_name: Hajny
orcid: 0000-0003-2140-7195
- first_name: Angharad
full_name: Jones, Angharad
last_name: Jones
- first_name: Sascha
full_name: Waidmann, Sascha
last_name: Waidmann
- first_name: Jürgen
full_name: Kleine Vehn, Jürgen
last_name: Kleine Vehn
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Grones P, Abas MF, Hajny J, et al. PID/WAG-mediated phosphorylation of the
Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism.
Scientific Reports. 2018;8(1). doi:10.1038/s41598-018-28188-1
apa: Grones, P., Abas, M. F., Hajny, J., Jones, A., Waidmann, S., Kleine Vehn, J.,
& Friml, J. (2018). PID/WAG-mediated phosphorylation of the Arabidopsis PIN3
auxin transporter mediates polarity switches during gravitropism. Scientific
Reports. Springer. https://doi.org/10.1038/s41598-018-28188-1
chicago: Grones, Peter, Melinda F Abas, Jakub Hajny, Angharad Jones, Sascha Waidmann,
Jürgen Kleine Vehn, and Jiří Friml. “PID/WAG-Mediated Phosphorylation of the Arabidopsis
PIN3 Auxin Transporter Mediates Polarity Switches during Gravitropism.” Scientific
Reports. Springer, 2018. https://doi.org/10.1038/s41598-018-28188-1.
ieee: P. Grones et al., “PID/WAG-mediated phosphorylation of the Arabidopsis
PIN3 auxin transporter mediates polarity switches during gravitropism,” Scientific
Reports, vol. 8, no. 1. Springer, 2018.
ista: Grones P, Abas MF, Hajny J, Jones A, Waidmann S, Kleine Vehn J, Friml J. 2018.
PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates
polarity switches during gravitropism. Scientific Reports. 8(1), 10279.
mla: Grones, Peter, et al. “PID/WAG-Mediated Phosphorylation of the Arabidopsis
PIN3 Auxin Transporter Mediates Polarity Switches during Gravitropism.” Scientific
Reports, vol. 8, no. 1, 10279, Springer, 2018, doi:10.1038/s41598-018-28188-1.
short: P. Grones, M.F. Abas, J. Hajny, A. Jones, S. Waidmann, J. Kleine Vehn, J.
Friml, Scientific Reports 8 (2018).
date_created: 2018-12-11T11:45:06Z
date_published: 2018-07-06T00:00:00Z
date_updated: 2024-03-18T23:30:39Z
day: '06'
ddc:
- '581'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1038/s41598-018-28188-1
ec_funded: 1
external_id:
isi:
- '000437673200053'
file:
- access_level: open_access
checksum: 266b03f4fb8198e83141617aaa99dcab
content_type: application/pdf
creator: dernst
date_created: 2018-12-17T15:38:56Z
date_updated: 2020-07-14T12:45:20Z
file_id: '5714'
file_name: 2018_ScientificReports_Grones.pdf
file_size: 2413876
relation: main_file
file_date_updated: 2020-07-14T12:45:20Z
has_accepted_license: '1'
intvolume: ' 8'
isi: 1
issue: '1'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Scientific Reports
publication_status: published
publisher: Springer
publist_id: '7729'
quality_controlled: '1'
related_material:
record:
- id: '8822'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter
mediates polarity switches during gravitropism
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 8
year: '2018'
...
---
_id: '442'
abstract:
- lang: eng
text: The rapid auxin-triggered growth of the Arabidopsis hypocotyls involves the
nuclear TIR1/AFB-Aux/IAA signaling and is accompanied by acidification of the
apoplast and cell walls (Fendrych et al., 2016). Here, we describe in detail the
method for analysis of the elongation and the TIR1/AFB-Aux/IAA-dependent auxin
response in hypocotyl segments as well as the determination of relative values
of the cell wall pH.
acknowledgement: 'This protocol was adapted from Fendrych et al., 2016. This project
has received funding from the European Union’s Horizon 2020 research and innovation
programme under the Marie Skłodowska-Curie Grant Agreement No. 665385, and Austrian
Science Fund (FWF) [M 2128-B21]. '
article_processing_charge: No
article_type: original
author:
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Gabriel
full_name: Krens, Gabriel
id: 2B819732-F248-11E8-B48F-1D18A9856A87
last_name: Krens
orcid: 0000-0003-4761-5996
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Li L, Krens G, Fendrych M, Friml J. Real-time analysis of auxin response, cell
wall pH and elongation in Arabidopsis thaliana Hypocotyls. Bio-protocol.
2018;8(1). doi:10.21769/BioProtoc.2685
apa: Li, L., Krens, G., Fendrych, M., & Friml, J. (2018). Real-time analysis
of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls.
Bio-Protocol. Bio-protocol. https://doi.org/10.21769/BioProtoc.2685
chicago: Li, Lanxin, Gabriel Krens, Matyas Fendrych, and Jiří Friml. “Real-Time
Analysis of Auxin Response, Cell Wall PH and Elongation in Arabidopsis Thaliana
Hypocotyls.” Bio-Protocol. Bio-protocol, 2018. https://doi.org/10.21769/BioProtoc.2685.
ieee: L. Li, G. Krens, M. Fendrych, and J. Friml, “Real-time analysis of auxin response,
cell wall pH and elongation in Arabidopsis thaliana Hypocotyls,” Bio-protocol,
vol. 8, no. 1. Bio-protocol, 2018.
ista: Li L, Krens G, Fendrych M, Friml J. 2018. Real-time analysis of auxin response,
cell wall pH and elongation in Arabidopsis thaliana Hypocotyls. Bio-protocol.
8(1).
mla: Li, Lanxin, et al. “Real-Time Analysis of Auxin Response, Cell Wall PH and
Elongation in Arabidopsis Thaliana Hypocotyls.” Bio-Protocol, vol. 8, no.
1, Bio-protocol, 2018, doi:10.21769/BioProtoc.2685.
short: L. Li, G. Krens, M. Fendrych, J. Friml, Bio-Protocol 8 (2018).
date_created: 2018-12-11T11:46:30Z
date_published: 2018-01-05T00:00:00Z
date_updated: 2024-03-18T23:30:45Z
day: '05'
ddc:
- '576'
- '581'
department:
- _id: JiFr
- _id: Bio
doi: 10.21769/BioProtoc.2685
ec_funded: 1
file:
- access_level: open_access
checksum: 6644ba698206eda32b0abf09128e63e3
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:17:43Z
date_updated: 2020-07-14T12:46:29Z
file_id: '5299'
file_name: IST-2018-970-v1+1_2018_Lanxin_Real-time_analysis.pdf
file_size: 11352389
relation: main_file
file_date_updated: 2020-07-14T12:46:29Z
has_accepted_license: '1'
intvolume: ' 8'
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
publication: Bio-protocol
publication_identifier:
eissn:
- 2331-8325
publication_status: published
publisher: Bio-protocol
publist_id: '7381'
pubrep_id: '970'
quality_controlled: '1'
related_material:
record:
- id: '10083'
relation: dissertation_contains
status: public
status: public
title: Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis
thaliana Hypocotyls
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 8
year: '2018'
...
---
_id: '572'
abstract:
- lang: eng
text: In this review, we summarize the different biosynthesis-related pathways that
contribute to the regulation of endogenous auxin in plants. We demonstrate that
all known genes involved in auxin biosynthesis also have a role in root formation,
from the initiation of a root meristem during embryogenesis to the generation
of a functional root system with a primary root, secondary lateral root branches
and adventitious roots. Furthermore, the versatile adaptation of root development
in response to environmental challenges is mediated by both local and distant
control of auxin biosynthesis. In conclusion, auxin homeostasis mediated by spatial
and temporal regulation of auxin biosynthesis plays a central role in determining
root architecture.
article_number: '2587'
article_processing_charge: No
author:
- first_name: Damilola
full_name: Olatunji, Damilola
last_name: Olatunji
- first_name: Danny
full_name: Geelen, Danny
last_name: Geelen
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
citation:
ama: Olatunji D, Geelen D, Verstraeten I. Control of endogenous auxin levels in
plant root development. International Journal of Molecular Sciences. 2017;18(12).
doi:10.3390/ijms18122587
apa: Olatunji, D., Geelen, D., & Verstraeten, I. (2017). Control of endogenous
auxin levels in plant root development. International Journal of Molecular
Sciences. MDPI. https://doi.org/10.3390/ijms18122587
chicago: Olatunji, Damilola, Danny Geelen, and Inge Verstraeten. “Control of Endogenous
Auxin Levels in Plant Root Development.” International Journal of Molecular
Sciences. MDPI, 2017. https://doi.org/10.3390/ijms18122587.
ieee: D. Olatunji, D. Geelen, and I. Verstraeten, “Control of endogenous auxin levels
in plant root development,” International Journal of Molecular Sciences,
vol. 18, no. 12. MDPI, 2017.
ista: Olatunji D, Geelen D, Verstraeten I. 2017. Control of endogenous auxin levels
in plant root development. International Journal of Molecular Sciences. 18(12),
2587.
mla: Olatunji, Damilola, et al. “Control of Endogenous Auxin Levels in Plant Root
Development.” International Journal of Molecular Sciences, vol. 18, no.
12, 2587, MDPI, 2017, doi:10.3390/ijms18122587.
short: D. Olatunji, D. Geelen, I. Verstraeten, International Journal of Molecular
Sciences 18 (2017).
date_created: 2018-12-11T11:47:15Z
date_published: 2017-12-01T00:00:00Z
date_updated: 2021-01-12T08:03:16Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.3390/ijms18122587
file:
- access_level: open_access
checksum: 82d51f11e493f7eec02976d9a9a9805e
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:08:55Z
date_updated: 2020-07-14T12:47:10Z
file_id: '4718'
file_name: IST-2017-917-v1+1_ijms-18-02587.pdf
file_size: 920962
relation: main_file
file_date_updated: 2020-07-14T12:47:10Z
has_accepted_license: '1'
intvolume: ' 18'
issue: '12'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
publication: International Journal of Molecular Sciences
publication_status: published
publisher: MDPI
publist_id: '7242'
pubrep_id: '917'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Control of endogenous auxin levels in plant root development
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 18
year: '2017'
...
---
_id: '657'
abstract:
- lang: eng
text: Plant organs are typically organized into three main tissue layers. The middle
ground tissue layer comprises the majority of the plant body and serves a wide
range of functions, including photosynthesis, selective nutrient uptake and storage,
and gravity sensing. Ground tissue patterning and maintenance in Arabidopsis are
controlled by a well-established gene network revolving around the key regulator
SHORT-ROOT (SHR). In contrast, it is completely unknown how ground tissue identity
is first specified from totipotent precursor cells in the embryo. The plant signaling
molecule auxin, acting through AUXIN RESPONSE FACTOR (ARF) transcription factors,
is critical for embryo patterning. The auxin effector ARF5/MONOPTEROS (MP) acts
both cell-autonomously and noncell-autonomously to control embryonic vascular
tissue formation and root initiation, respectively. Here we show that auxin response
and ARF activity cell-autonomously control the asymmetric division of the first
ground tissue cells. By identifying embryonic target genes, we show that MP transcriptionally
initiates the ground tissue lineage and acts upstream of the regulatory network
that controls ground tissue patterning and maintenance. Strikingly, whereas the
SHR network depends on MP, this MP function is, at least in part, SHR independent.
Our study therefore identifies auxin response as a regulator of ground tissue
specification in the embryonic root, and reveals that ground tissue initiation
and maintenance use different regulators and mechanisms. Moreover, our data provide
a framework for the simultaneous formation of multiple cell types by the same
transcriptional regulator.
author:
- first_name: Barbara
full_name: Möller, Barbara
last_name: Möller
- first_name: Colette
full_name: Ten Hove, Colette
last_name: Ten Hove
- first_name: Daoquan
full_name: Xiang, Daoquan
last_name: Xiang
- first_name: Nerys
full_name: Williams, Nerys
last_name: Williams
- first_name: Lorena
full_name: López, Lorena
last_name: López
- first_name: Saiko
full_name: Yoshida, Saiko
id: 2E46069C-F248-11E8-B48F-1D18A9856A87
last_name: Yoshida
- first_name: Margot
full_name: Smit, Margot
last_name: Smit
- first_name: Raju
full_name: Datla, Raju
last_name: Datla
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
citation:
ama: Möller B, Ten Hove C, Xiang D, et al. Auxin response cell autonomously controls
ground tissue initiation in the early arabidopsis embryo. PNAS. 2017;114(12):E2533-E2539.
doi:10.1073/pnas.1616493114
apa: Möller, B., Ten Hove, C., Xiang, D., Williams, N., López, L., Yoshida, S.,
… Weijers, D. (2017). Auxin response cell autonomously controls ground tissue
initiation in the early arabidopsis embryo. PNAS. National Academy of Sciences.
https://doi.org/10.1073/pnas.1616493114
chicago: Möller, Barbara, Colette Ten Hove, Daoquan Xiang, Nerys Williams, Lorena
López, Saiko Yoshida, Margot Smit, Raju Datla, and Dolf Weijers. “Auxin Response
Cell Autonomously Controls Ground Tissue Initiation in the Early Arabidopsis Embryo.”
PNAS. National Academy of Sciences, 2017. https://doi.org/10.1073/pnas.1616493114.
ieee: B. Möller et al., “Auxin response cell autonomously controls ground
tissue initiation in the early arabidopsis embryo,” PNAS, vol. 114, no.
12. National Academy of Sciences, pp. E2533–E2539, 2017.
ista: Möller B, Ten Hove C, Xiang D, Williams N, López L, Yoshida S, Smit M, Datla
R, Weijers D. 2017. Auxin response cell autonomously controls ground tissue initiation
in the early arabidopsis embryo. PNAS. 114(12), E2533–E2539.
mla: Möller, Barbara, et al. “Auxin Response Cell Autonomously Controls Ground Tissue
Initiation in the Early Arabidopsis Embryo.” PNAS, vol. 114, no. 12, National
Academy of Sciences, 2017, pp. E2533–39, doi:10.1073/pnas.1616493114.
short: B. Möller, C. Ten Hove, D. Xiang, N. Williams, L. López, S. Yoshida, M. Smit,
R. Datla, D. Weijers, PNAS 114 (2017) E2533–E2539.
date_created: 2018-12-11T11:47:45Z
date_published: 2017-03-21T00:00:00Z
date_updated: 2021-01-12T08:08:02Z
day: '21'
department:
- _id: JiFr
doi: 10.1073/pnas.1616493114
external_id:
pmid:
- '28265057'
intvolume: ' 114'
issue: '12'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5373392/
month: '03'
oa: 1
oa_version: Submitted Version
page: E2533 - E2539
pmid: 1
publication: PNAS
publication_identifier:
issn:
- '00278424'
publication_status: published
publisher: National Academy of Sciences
publist_id: '7076'
quality_controlled: '1'
scopus_import: 1
status: public
title: Auxin response cell autonomously controls ground tissue initiation in the early
arabidopsis embryo
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 114
year: '2017'
...
---
_id: '669'
abstract:
- lang: eng
text: 'The exocyst, a eukaryotic tethering complex, coregulates targeted exocytosis
as an effector of small GTPases in polarized cell growth. In land plants, several
exocyst subunits are encoded by double or triple paralogs, culminating in tens
of EXO70 paralogs. Out of 23 Arabidopsis thaliana EXO70 isoforms, we analyzed
seven isoforms expressed in pollen. Genetic and microscopic analyses of single
mutants in EXO70A2, EXO70C1, EXO70C2, EXO70F1, EXO70H3, EXO70H5, and EXO70H6 genes
revealed that only a loss-of-function EXO70C2 allele resulted in a significant
male-specific transmission defect (segregation 40%:51%:9%) due to aberrant pollen
tube growth. Mutant pollen tubes grown in vitro exhibited an enhanced growth rate
and a decreased thickness of the tip cell wall, causing tip bursts. However, exo70C2
pollen tubes could frequently recover and restart their speedy elongation, resulting
in a repetitive stop-and-go growth dynamics. A pollenspecific depletion of the
closest paralog, EXO70C1, using artificial microRNA in the exo70C2 mutant background,
resulted in a complete pollen-specific transmission defect, suggesting redundant
functions of EXO70C1 and EXO70C2. Both EXO70C1 and EXO70C2, GFP tagged and expressed
under the control of their native promoters, localized in the cytoplasm of pollen
grains, pollen tubes, and also root trichoblast cells. The expression of EXO70C2-GFP
complemented the aberrant growth of exo70C2 pollen tubes. The absent EXO70C2 interactions
with core exocyst subunits in the yeast two-hybrid assay, cytoplasmic localization,
and genetic effect suggest an unconventional EXO70 function possibly as a regulator
of exocytosis outside the exocyst complex. In conclusion, EXO70C2 is a novel factor
contributing to the regulation of optimal tip growth of Arabidopsis pollen tubes. '
article_processing_charge: No
article_type: original
author:
- first_name: Lukáš
full_name: Synek, Lukáš
last_name: Synek
- first_name: Nemanja
full_name: Vukašinović, Nemanja
last_name: Vukašinović
- first_name: Ivan
full_name: Kulich, Ivan
last_name: Kulich
- first_name: Michal
full_name: Hála, Michal
last_name: Hála
- first_name: Klára
full_name: Aldorfová, Klára
last_name: Aldorfová
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: Viktor
full_name: Žárský, Viktor
last_name: Žárský
citation:
ama: Synek L, Vukašinović N, Kulich I, et al. EXO70C2 is a key regulatory factor
for optimal tip growth of pollen. Plant Physiology. 2017;174(1):223-240.
doi:10.1104/pp.16.01282
apa: Synek, L., Vukašinović, N., Kulich, I., Hála, M., Aldorfová, K., Fendrych,
M., & Žárský, V. (2017). EXO70C2 is a key regulatory factor for optimal tip
growth of pollen. Plant Physiology. American Society of Plant Biologists.
https://doi.org/10.1104/pp.16.01282
chicago: Synek, Lukáš, Nemanja Vukašinović, Ivan Kulich, Michal Hála, Klára Aldorfová,
Matyas Fendrych, and Viktor Žárský. “EXO70C2 Is a Key Regulatory Factor for Optimal
Tip Growth of Pollen.” Plant Physiology. American Society of Plant Biologists,
2017. https://doi.org/10.1104/pp.16.01282.
ieee: L. Synek et al., “EXO70C2 is a key regulatory factor for optimal tip
growth of pollen,” Plant Physiology, vol. 174, no. 1. American Society
of Plant Biologists, pp. 223–240, 2017.
ista: Synek L, Vukašinović N, Kulich I, Hála M, Aldorfová K, Fendrych M, Žárský
V. 2017. EXO70C2 is a key regulatory factor for optimal tip growth of pollen.
Plant Physiology. 174(1), 223–240.
mla: Synek, Lukáš, et al. “EXO70C2 Is a Key Regulatory Factor for Optimal Tip Growth
of Pollen.” Plant Physiology, vol. 174, no. 1, American Society of Plant
Biologists, 2017, pp. 223–40, doi:10.1104/pp.16.01282.
short: L. Synek, N. Vukašinović, I. Kulich, M. Hála, K. Aldorfová, M. Fendrych,
V. Žárský, Plant Physiology 174 (2017) 223–240.
date_created: 2018-12-11T11:47:49Z
date_published: 2017-05-01T00:00:00Z
date_updated: 2021-01-12T08:08:35Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1104/pp.16.01282
external_id:
pmid:
- '28356503'
file:
- access_level: open_access
checksum: 97155acc6aa5f0d0a78e0589a932fe02
content_type: application/pdf
creator: dernst
date_created: 2019-11-18T16:16:18Z
date_updated: 2020-07-14T12:47:37Z
file_id: '7041'
file_name: 2017_PlantPhysio_Synek.pdf
file_size: 2176903
relation: main_file
file_date_updated: 2020-07-14T12:47:37Z
has_accepted_license: '1'
intvolume: ' 174'
issue: '1'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Submitted Version
page: 223 - 240
pmid: 1
publication: Plant Physiology
publication_identifier:
issn:
- '00320889'
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '7058'
quality_controlled: '1'
scopus_import: 1
status: public
title: EXO70C2 is a key regulatory factor for optimal tip growth of pollen
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 174
year: '2017'
...
---
_id: '722'
abstract:
- lang: eng
text: Plants are sessile organisms rooted in one place. The soil resources that
plants require are often distributed in a highly heterogeneous pattern. To aid
foraging, plants have evolved roots whose growth and development are highly responsive
to soil signals. As a result, 3D root architecture is shaped by myriad environmental
signals to ensure resource capture is optimised and unfavourable environments
are avoided. The first signals sensed by newly germinating seeds — gravity and
light — direct root growth into the soil to aid seedling establishment. Heterogeneous
soil resources, such as water, nitrogen and phosphate, also act as signals that
shape 3D root growth to optimise uptake. Root architecture is also modified through
biotic interactions that include soil fungi and neighbouring plants. This developmental
plasticity results in a ‘custom-made’ 3D root system that is best adapted to forage
for resources in each soil environment that a plant colonises.
author:
- first_name: Emily
full_name: Morris, Emily
last_name: Morris
- first_name: Marcus
full_name: Griffiths, Marcus
last_name: Griffiths
- first_name: Agata
full_name: Golebiowska, Agata
last_name: Golebiowska
- first_name: Stefan
full_name: Mairhofer, Stefan
last_name: Mairhofer
- first_name: Jasmine
full_name: Burr Hersey, Jasmine
last_name: Burr Hersey
- first_name: Tatsuaki
full_name: Goh, Tatsuaki
last_name: Goh
- first_name: Daniel
full_name: Von Wangenheim, Daniel
id: 49E91952-F248-11E8-B48F-1D18A9856A87
last_name: Von Wangenheim
orcid: 0000-0002-6862-1247
- first_name: Brian
full_name: Atkinson, Brian
last_name: Atkinson
- first_name: Craig
full_name: Sturrock, Craig
last_name: Sturrock
- first_name: Jonathan
full_name: Lynch, Jonathan
last_name: Lynch
- first_name: Kris
full_name: Vissenberg, Kris
last_name: Vissenberg
- first_name: Karl
full_name: Ritz, Karl
last_name: Ritz
- first_name: Darren
full_name: Wells, Darren
last_name: Wells
- first_name: Sacha
full_name: Mooney, Sacha
last_name: Mooney
- first_name: Malcolm
full_name: Bennett, Malcolm
last_name: Bennett
citation:
ama: Morris E, Griffiths M, Golebiowska A, et al. Shaping 3D root system architecture.
Current Biology. 2017;27(17):R919-R930. doi:10.1016/j.cub.2017.06.043
apa: Morris, E., Griffiths, M., Golebiowska, A., Mairhofer, S., Burr Hersey, J.,
Goh, T., … Bennett, M. (2017). Shaping 3D root system architecture. Current
Biology. Cell Press. https://doi.org/10.1016/j.cub.2017.06.043
chicago: Morris, Emily, Marcus Griffiths, Agata Golebiowska, Stefan Mairhofer, Jasmine
Burr Hersey, Tatsuaki Goh, Daniel von Wangenheim, et al. “Shaping 3D Root System
Architecture.” Current Biology. Cell Press, 2017. https://doi.org/10.1016/j.cub.2017.06.043.
ieee: E. Morris et al., “Shaping 3D root system architecture,” Current
Biology, vol. 27, no. 17. Cell Press, pp. R919–R930, 2017.
ista: Morris E, Griffiths M, Golebiowska A, Mairhofer S, Burr Hersey J, Goh T, von
Wangenheim D, Atkinson B, Sturrock C, Lynch J, Vissenberg K, Ritz K, Wells D,
Mooney S, Bennett M. 2017. Shaping 3D root system architecture. Current Biology.
27(17), R919–R930.
mla: Morris, Emily, et al. “Shaping 3D Root System Architecture.” Current Biology,
vol. 27, no. 17, Cell Press, 2017, pp. R919–30, doi:10.1016/j.cub.2017.06.043.
short: E. Morris, M. Griffiths, A. Golebiowska, S. Mairhofer, J. Burr Hersey, T.
Goh, D. von Wangenheim, B. Atkinson, C. Sturrock, J. Lynch, K. Vissenberg, K.
Ritz, D. Wells, S. Mooney, M. Bennett, Current Biology 27 (2017) R919–R930.
date_created: 2018-12-11T11:48:08Z
date_published: 2017-09-11T00:00:00Z
date_updated: 2021-01-12T08:12:29Z
day: '11'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.1016/j.cub.2017.06.043
ec_funded: 1
external_id:
pmid:
- '28898665'
file:
- access_level: open_access
checksum: e45588b21097b408da6276a3e5eedb2e
content_type: application/pdf
creator: dernst
date_created: 2019-04-17T07:46:40Z
date_updated: 2020-07-14T12:47:54Z
file_id: '6332'
file_name: 2017_CurrentBiology_Morris.pdf
file_size: 1576593
relation: main_file
file_date_updated: 2020-07-14T12:47:54Z
has_accepted_license: '1'
intvolume: ' 27'
issue: '17'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Submitted Version
page: R919 - R930
pmid: 1
project:
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
publication: Current Biology
publication_identifier:
issn:
- '09609822'
publication_status: published
publisher: Cell Press
publist_id: '6956'
pubrep_id: '982'
quality_controlled: '1'
scopus_import: 1
status: public
title: Shaping 3D root system architecture
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 27
year: '2017'
...