---
_id: '14826'
abstract:
- lang: eng
text: The plant-signaling molecule auxin triggers fast and slow cellular responses
across land plants and algae. The nuclear auxin pathway mediates gene expression
and controls growth and development in land plants, but this pathway is absent
from algal sister groups. Several components of rapid responses have been identified
in Arabidopsis, but it is unknown if these are part of a conserved mechanism.
We recently identified a fast, proteome-wide phosphorylation response to auxin.
Here, we show that this response occurs across 5 land plant and algal species
and converges on a core group of shared targets. We found conserved rapid physiological
responses to auxin in the same species and identified rapidly accelerated fibrosarcoma
(RAF)-like protein kinases as central mediators of auxin-triggered phosphorylation
across species. Genetic analysis connects this kinase to both auxin-triggered
protein phosphorylation and rapid cellular response, thus identifying an ancient
mechanism for fast auxin responses in the green lineage.
acknowledgement: 'We are grateful to Asuka Shitaku and Eri Koide for generating and
sharing the Marchantia PRAF-mCitrine line and Peng-Cheng Wang for sharing the Arabidopsis
raf mutant. We are grateful to our team members for discussions and helpful advice.
This work was supported by funding from the Netherlands Organization for Scientific
Research (NWO): VICI grant 865.14.001 and ENW-KLEIN OCENW.KLEIN.027 grants to D.W.;
VENI grant VI.VENI.212.003 to A.K.; the European Research Council AdG DIRNDL (contract
number 833867) to D.W.; CoG CATCH to J.S.; StG CELLONGATE (contract 803048) to M.F.;
and AdG ETAP (contract 742985) to J.F.; MEXT KAKENHI grant number JP19H05675 to
T.K.; JSPS KAKENHI grant number JP20H03275 to R.N.; Takeda Science Foundation to
R.N.; and the Austrian Science Fund (FWF, P29988) to J.F.'
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Andre
full_name: Kuhn, Andre
last_name: Kuhn
- first_name: Mark
full_name: Roosjen, Mark
last_name: Roosjen
- first_name: Sumanth
full_name: Mutte, Sumanth
last_name: Mutte
- first_name: Shiv Mani
full_name: Dubey, Shiv Mani
last_name: Dubey
- first_name: Vanessa Polet
full_name: Carrillo Carrasco, Vanessa Polet
last_name: Carrillo Carrasco
- first_name: Sjef
full_name: Boeren, Sjef
last_name: Boeren
- first_name: Aline
full_name: Monzer, Aline
id: 2DB5D88C-D7B3-11E9-B8FD-7907E6697425
last_name: Monzer
- first_name: Jasper
full_name: Koehorst, Jasper
last_name: Koehorst
- first_name: Takayuki
full_name: Kohchi, Takayuki
last_name: Kohchi
- first_name: Ryuichi
full_name: Nishihama, Ryuichi
last_name: Nishihama
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: Joris
full_name: Sprakel, Joris
last_name: Sprakel
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
citation:
ama: Kuhn A, Roosjen M, Mutte S, et al. RAF-like protein kinases mediate a deeply
conserved, rapid auxin response. Cell. 2024;187(1):130-148.e17. doi:10.1016/j.cell.2023.11.021
apa: Kuhn, A., Roosjen, M., Mutte, S., Dubey, S. M., Carrillo Carrasco, V. P., Boeren,
S., … Weijers, D. (2024). RAF-like protein kinases mediate a deeply conserved,
rapid auxin response. Cell. Elsevier. https://doi.org/10.1016/j.cell.2023.11.021
chicago: Kuhn, Andre, Mark Roosjen, Sumanth Mutte, Shiv Mani Dubey, Vanessa Polet
Carrillo Carrasco, Sjef Boeren, Aline Monzer, et al. “RAF-like Protein Kinases
Mediate a Deeply Conserved, Rapid Auxin Response.” Cell. Elsevier, 2024.
https://doi.org/10.1016/j.cell.2023.11.021.
ieee: A. Kuhn et al., “RAF-like protein kinases mediate a deeply conserved,
rapid auxin response,” Cell, vol. 187, no. 1. Elsevier, p. 130–148.e17,
2024.
ista: Kuhn A, Roosjen M, Mutte S, Dubey SM, Carrillo Carrasco VP, Boeren S, Monzer
A, Koehorst J, Kohchi T, Nishihama R, Fendrych M, Sprakel J, Friml J, Weijers
D. 2024. RAF-like protein kinases mediate a deeply conserved, rapid auxin response.
Cell. 187(1), 130–148.e17.
mla: Kuhn, Andre, et al. “RAF-like Protein Kinases Mediate a Deeply Conserved, Rapid
Auxin Response.” Cell, vol. 187, no. 1, Elsevier, 2024, p. 130–148.e17,
doi:10.1016/j.cell.2023.11.021.
short: A. Kuhn, M. Roosjen, S. Mutte, S.M. Dubey, V.P. Carrillo Carrasco, S. Boeren,
A. Monzer, J. Koehorst, T. Kohchi, R. Nishihama, M. Fendrych, J. Sprakel, J. Friml,
D. Weijers, Cell 187 (2024) 130–148.e17.
date_created: 2024-01-17T12:45:40Z
date_published: 2024-01-04T00:00:00Z
date_updated: 2024-01-22T13:43:40Z
day: '04'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.cell.2023.11.021
ec_funded: 1
external_id:
pmid:
- '38128538'
file:
- access_level: open_access
checksum: 06fd236a9ee0b46ccb05f44695bfc34b
content_type: application/pdf
creator: dernst
date_created: 2024-01-22T13:41:41Z
date_updated: 2024-01-22T13:41:41Z
file_id: '14874'
file_name: 2024_Cell_Kuhn.pdf
file_size: 13194060
relation: main_file
success: 1
file_date_updated: 2024-01-22T13:41:41Z
has_accepted_license: '1'
intvolume: ' 187'
issue: '1'
keyword:
- General Biochemistry
- Genetics and Molecular Biology
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc/4.0/
month: '01'
oa: 1
oa_version: Published Version
page: 130-148.e17
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 262EF96E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29988
name: RNA-directed DNA methylation in plant development
publication: Cell
publication_identifier:
eissn:
- 1097-4172
issn:
- 0092-8674
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: RAF-like protein kinases mediate a deeply conserved, rapid auxin response
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 187
year: '2024'
...
---
_id: '14251'
abstract:
- lang: eng
text: The phytohormone auxin and its directional transport through tissues play
a fundamental role in development of higher plants. This polar auxin transport
predominantly relies on PIN-FORMED (PIN) auxin exporters. Hence, PIN polarization
is crucial for development, but its evolution during the rise of morphological
complexity in land plants remains unclear. Here, we performed a cross-species
investigation by observing the trafficking and localization of endogenous and
exogenous PINs in two bryophytes, Physcomitrium patens and Marchantia polymorpha,
and in the flowering plant Arabidopsis thaliana. We confirmed that the GFP fusion
did not compromise the auxin export function of all examined PINs by using radioactive
auxin export assay and by observing the phenotypic changes in transgenic bryophytes.
Endogenous PINs polarize to filamentous apices, while exogenous Arabidopsis PINs
distribute symmetrically on the membrane in both bryophytes. In Arabidopsis root
epidermis, bryophytic PINs show no defined polarity. Pharmacological interference
revealed a strong cytoskeleton dependence of bryophytic but not Arabidopsis PIN
polarization. The divergence of PIN polarization and trafficking is also observed
within the bryophyte clade and between tissues of individual species. These results
collectively reveal a divergence of PIN trafficking and polarity mechanisms throughout
land plant evolution and a co-evolution of PIN sequence-based and cell-based polarity
mechanisms.
acknowledgement: This work was supported by the ERC grant (PR1023ERC02) to H. T. and
J. F., and by the ministry of science and technology (grant number 110-2636-B-005-001)
to K. J. L.
article_number: '100669'
article_processing_charge: Yes
article_type: original
author:
- first_name: Han
full_name: Tang, Han
id: 19BDF720-25A0-11EA-AC6E-928F3DDC885E
last_name: Tang
orcid: 0000-0001-6152-6637
- first_name: KJ
full_name: Lu, KJ
last_name: Lu
- first_name: Y
full_name: Zhang, Y
last_name: Zhang
- first_name: YL
full_name: Cheng, YL
last_name: Cheng
- first_name: SL
full_name: Tu, SL
last_name: Tu
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Tang H, Lu K, Zhang Y, Cheng Y, Tu S, Friml J. Divergence of trafficking and
polarization mechanisms for PIN auxin transporters during land plant evolution.
Plant Communications. 2024;5(1). doi:10.1016/j.xplc.2023.100669
apa: Tang, H., Lu, K., Zhang, Y., Cheng, Y., Tu, S., & Friml, J. (2024). Divergence
of trafficking and polarization mechanisms for PIN auxin transporters during land
plant evolution. Plant Communications. Elsevier. https://doi.org/10.1016/j.xplc.2023.100669
chicago: Tang, Han, KJ Lu, Y Zhang, YL Cheng, SL Tu, and Jiří Friml. “Divergence
of Trafficking and Polarization Mechanisms for PIN Auxin Transporters during Land
Plant Evolution.” Plant Communications. Elsevier, 2024. https://doi.org/10.1016/j.xplc.2023.100669.
ieee: H. Tang, K. Lu, Y. Zhang, Y. Cheng, S. Tu, and J. Friml, “Divergence of trafficking
and polarization mechanisms for PIN auxin transporters during land plant evolution,”
Plant Communications, vol. 5, no. 1. Elsevier, 2024.
ista: Tang H, Lu K, Zhang Y, Cheng Y, Tu S, Friml J. 2024. Divergence of trafficking
and polarization mechanisms for PIN auxin transporters during land plant evolution.
Plant Communications. 5(1), 100669.
mla: Tang, Han, et al. “Divergence of Trafficking and Polarization Mechanisms for
PIN Auxin Transporters during Land Plant Evolution.” Plant Communications,
vol. 5, no. 1, 100669, Elsevier, 2024, doi:10.1016/j.xplc.2023.100669.
short: H. Tang, K. Lu, Y. Zhang, Y. Cheng, S. Tu, J. Friml, Plant Communications
5 (2024).
date_created: 2023-09-01T11:32:02Z
date_published: 2024-01-08T00:00:00Z
date_updated: 2024-01-30T13:00:47Z
day: '08'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.xplc.2023.100669
ec_funded: 1
external_id:
pmid:
- '37528584'
file:
- access_level: open_access
checksum: edbc44c6d4a394d2bf70f92fdbb08f0a
content_type: application/pdf
creator: dernst
date_created: 2024-01-30T12:59:57Z
date_updated: 2024-01-30T12:59:57Z
file_id: '14911'
file_name: 2023_PlantCommunications_Tang.pdf
file_size: 2825565
relation: main_file
success: 1
file_date_updated: 2024-01-30T12:59:57Z
has_accepted_license: '1'
intvolume: ' 5'
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Plant Communications
publication_identifier:
issn:
- 2590-3462
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Divergence of trafficking and polarization mechanisms for PIN auxin transporters
during land plant evolution
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 5
year: '2024'
...
---
_id: '15033'
abstract:
- lang: eng
text: The GNOM (GN) Guanine nucleotide Exchange Factor for ARF small GTPases (ARF-GEF)
is among the best studied trafficking regulators in plants, playing crucial and
unique developmental roles in patterning and polarity. The current models place
GN at the Golgi apparatus (GA), where it mediates secretion/recycling, and at
the plasma membrane (PM) presumably contributing to clathrin-mediated endocytosis
(CME). The mechanistic basis of the developmental function of GN, distinct from
the other ARF-GEFs including its closest homologue GNOM-LIKE1 (GNL1), remains
elusive. Insights from this study largely extend the current notions of GN function.
We show that GN, but not GNL1, localizes to the cell periphery at long-lived structures
distinct from clathrin-coated pits, while CME and secretion proceed normally in
gn knockouts. The functional GN mutant variant GNfewerroots,
absent from the GA, suggests that the cell periphery is the major site of GN action
responsible for its developmental function. Following inhibition by Brefeldin
A, GN, but not GNL1, relocates to the PM likely on exocytic vesicles, suggesting
selective molecular associations en route to the cell periphery. A study of GN-GNL1
chimeric ARF-GEFs indicates that all GN domains contribute to the specific GN
function in a partially redundant manner. Together, this study offers significant
steps toward the elucidation of the mechanism underlying unique cellular and development
functions of GNOM.
acknowledgement: "The authors would like to gratefully acknowledge Dr Xixi Zhang for
cloning the GNL1/pDONR221 construct and for useful discussions.H2020 European Research\r\nCouncil
Advanced Grant ETAP742985 to Jiří Friml, Austrian Science Fund I 3630-B25 to Jiří
Friml"
article_processing_charge: Yes
article_type: original
author:
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Ivana
full_name: Matijevic, Ivana
id: 83c17ce3-15b2-11ec-abd3-f486545870bd
last_name: Matijevic
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Adamowski M, Matijevic I, Friml J. Developmental patterning function of GNOM
ARF-GEF mediated from the cell periphery. eLife. 2024;13. doi:10.7554/elife.68993
apa: Adamowski, M., Matijevic, I., & Friml, J. (2024). Developmental patterning
function of GNOM ARF-GEF mediated from the cell periphery. ELife. eLife
Sciences Publications. https://doi.org/10.7554/elife.68993
chicago: Adamowski, Maciek, Ivana Matijevic, and Jiří Friml. “Developmental Patterning
Function of GNOM ARF-GEF Mediated from the Cell Periphery.” ELife. eLife
Sciences Publications, 2024. https://doi.org/10.7554/elife.68993.
ieee: M. Adamowski, I. Matijevic, and J. Friml, “Developmental patterning function
of GNOM ARF-GEF mediated from the cell periphery,” eLife, vol. 13. eLife
Sciences Publications, 2024.
ista: Adamowski M, Matijevic I, Friml J. 2024. Developmental patterning function
of GNOM ARF-GEF mediated from the cell periphery. eLife. 13.
mla: Adamowski, Maciek, et al. “Developmental Patterning Function of GNOM ARF-GEF
Mediated from the Cell Periphery.” ELife, vol. 13, eLife Sciences Publications,
2024, doi:10.7554/elife.68993.
short: M. Adamowski, I. Matijevic, J. Friml, ELife 13 (2024).
date_created: 2024-02-27T07:10:11Z
date_published: 2024-02-21T00:00:00Z
date_updated: 2024-02-28T12:29:43Z
day: '21'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.7554/elife.68993
ec_funded: 1
has_accepted_license: '1'
intvolume: ' 13'
keyword:
- General Immunology and Microbiology
- General Biochemistry
- Genetics and Molecular Biology
- General Medicine
- General Neuroscience
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.7554/eLife.68993
month: '02'
oa: 1
oa_version: Published Version
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: eLife
publication_identifier:
issn:
- 2050-084X
publication_status: epub_ahead
publisher: eLife Sciences Publications
quality_controlled: '1'
status: public
title: Developmental patterning function of GNOM ARF-GEF mediated from the cell periphery
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 13
year: '2024'
...
---
_id: '12878'
abstract:
- lang: eng
text: Salicylic acid (SA) plays important roles in different aspects of plant development,
including root growth, where auxin is also a major player by means of its asymmetric
distribution. However, the mechanism underlying the effect of SA on the development
of rice roots remains poorly understood. Here, we show that SA inhibits rice root
growth by interfering with auxin transport associated with the OsPIN3t- and clathrin-mediated
gene regulatory network (GRN). SA inhibits root growth as well as Brefeldin A-sensitive
trafficking through a non-canonical SA signaling mechanism. Transcriptome analysis
of rice seedlings treated with SA revealed that the OsPIN3t auxin transporter
is at the center of a GRN involving the coat protein clathrin. The root growth
and endocytic trafficking in both the pin3t and clathrin heavy chain mutants were
SA insensitivity. SA inhibitory effect on the endocytosis of OsPIN3t was dependent
on clathrin; however, the root growth and endocytic trafficking mediated by tyrphostin
A23 (TyrA23) were independent of the pin3t mutant under SA treatment. These data
reveal that SA affects rice root growth through the convergence of transcriptional
and non-SA signaling mechanisms involving OsPIN3t-mediated auxin transport and
clathrin-mediated trafficking as key components.
acknowledgement: The authors thank Professor Jianqiang Wu (Kunming Institute of Botany,
Chinese Academy of Sciences) for support with phytohormone measurement. Thanks also
go to Professor Pieter. B. F. Ouwerkerk (Leiden University) and Professor Jean-Benoit
Morel (Plant Health Institute of Montpellier) for provision of the rice lines NB-7B-70
and NB-7B-76 and wild-type NB-61-WT, Professor Zuhua He (Chinese Academy of Sciences)
for provision of the rice OsNPR1-RNAi mutant, and Professor Yinong Yang (The Pennsylvania
State University) for provision of the rice line NahG. This work was supported by
grants from the National Natural Science Foundation of China (Grant Nos. 32260085,
31460453, 31660501, 31860064, 31970609, 31801792 and 31960554), the Key Projects
of the Applied Basic Research Plan of Yunnan Province (202301AS070082), the Major
Special Program for Scientific Research, Education Department of Yunnan Province
(Grant No. ZD2015005), the Start-up fund from Xishuangbanna Tropical Botanical Garden,
and ‘Top Talents Program in Science and Technology’ from Yunnan Province, the SRF
for ROCS, SEM (Grant No. [2013] 1792), and the Major Science and Technology Project
in Yunnan Province (202102AE090042 and 202202AE090036); and the young and middle-aged
academic and technical leaders reserve talent program in Yunnan Province (202205AC160076).
article_processing_charge: No
article_type: original
author:
- first_name: Lihui
full_name: Jiang, Lihui
last_name: Jiang
- first_name: Baolin
full_name: Yao, Baolin
last_name: Yao
- first_name: Xiaoyan
full_name: Zhang, Xiaoyan
last_name: Zhang
- first_name: Lixia
full_name: Wu, Lixia
last_name: Wu
- first_name: Qijing
full_name: Fu, Qijing
last_name: Fu
- first_name: Yiting
full_name: Zhao, Yiting
last_name: Zhao
- first_name: Yuxin
full_name: Cao, Yuxin
last_name: Cao
- first_name: Ruomeng
full_name: Zhu, Ruomeng
last_name: Zhu
- first_name: Xinqi
full_name: Lu, Xinqi
last_name: Lu
- first_name: Wuying
full_name: Huang, Wuying
last_name: Huang
- first_name: Jianping
full_name: Zhao, Jianping
last_name: Zhao
- first_name: Kuixiu
full_name: Li, Kuixiu
last_name: Li
- first_name: Shuanglu
full_name: Zhao, Shuanglu
last_name: Zhao
- first_name: Li
full_name: Han, Li
last_name: Han
- first_name: Xuan
full_name: Zhou, Xuan
last_name: Zhou
- first_name: Chongyu
full_name: Luo, Chongyu
last_name: Luo
- first_name: Haiyan
full_name: Zhu, Haiyan
last_name: Zhu
- first_name: Jing
full_name: Yang, Jing
last_name: Yang
- first_name: Huichuan
full_name: Huang, Huichuan
last_name: Huang
- first_name: Zhengge
full_name: Zhu, Zhengge
last_name: Zhu
- first_name: Xiahong
full_name: He, Xiahong
last_name: He
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Zhongkai
full_name: Zhang, Zhongkai
last_name: Zhang
- first_name: Changning
full_name: Liu, Changning
last_name: Liu
- first_name: Yunlong
full_name: Du, Yunlong
last_name: Du
citation:
ama: Jiang L, Yao B, Zhang X, et al. Salicylic acid inhibits rice endocytic protein
trafficking mediated by OsPIN3t and clathrin to affect root growth. Plant Journal.
2023;115(1):155-174. doi:10.1111/tpj.16218
apa: Jiang, L., Yao, B., Zhang, X., Wu, L., Fu, Q., Zhao, Y., … Du, Y. (2023). Salicylic
acid inhibits rice endocytic protein trafficking mediated by OsPIN3t and clathrin
to affect root growth. Plant Journal. Wiley. https://doi.org/10.1111/tpj.16218
chicago: Jiang, Lihui, Baolin Yao, Xiaoyan Zhang, Lixia Wu, Qijing Fu, Yiting Zhao,
Yuxin Cao, et al. “Salicylic Acid Inhibits Rice Endocytic Protein Trafficking
Mediated by OsPIN3t and Clathrin to Affect Root Growth.” Plant Journal.
Wiley, 2023. https://doi.org/10.1111/tpj.16218.
ieee: L. Jiang et al., “Salicylic acid inhibits rice endocytic protein trafficking
mediated by OsPIN3t and clathrin to affect root growth,” Plant Journal,
vol. 115, no. 1. Wiley, pp. 155–174, 2023.
ista: Jiang L, Yao B, Zhang X, Wu L, Fu Q, Zhao Y, Cao Y, Zhu R, Lu X, Huang W,
Zhao J, Li K, Zhao S, Han L, Zhou X, Luo C, Zhu H, Yang J, Huang H, Zhu Z, He
X, Friml J, Zhang Z, Liu C, Du Y. 2023. Salicylic acid inhibits rice endocytic
protein trafficking mediated by OsPIN3t and clathrin to affect root growth. Plant
Journal. 115(1), 155–174.
mla: Jiang, Lihui, et al. “Salicylic Acid Inhibits Rice Endocytic Protein Trafficking
Mediated by OsPIN3t and Clathrin to Affect Root Growth.” Plant Journal,
vol. 115, no. 1, Wiley, 2023, pp. 155–74, doi:10.1111/tpj.16218.
short: L. Jiang, B. Yao, X. Zhang, L. Wu, Q. Fu, Y. Zhao, Y. Cao, R. Zhu, X. Lu,
W. Huang, J. Zhao, K. Li, S. Zhao, L. Han, X. Zhou, C. Luo, H. Zhu, J. Yang, H.
Huang, Z. Zhu, X. He, J. Friml, Z. Zhang, C. Liu, Y. Du, Plant Journal 115 (2023)
155–174.
date_created: 2023-04-30T22:01:06Z
date_published: 2023-07-01T00:00:00Z
date_updated: 2023-08-01T14:16:33Z
day: '01'
department:
- _id: JiFr
doi: 10.1111/tpj.16218
external_id:
isi:
- '000971861400001'
pmid:
- '37025008 '
intvolume: ' 115'
isi: 1
issue: '1'
language:
- iso: eng
month: '07'
oa_version: None
page: 155-174
pmid: 1
publication: Plant Journal
publication_identifier:
eissn:
- 1365-313X
issn:
- 0960-7412
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Salicylic acid inhibits rice endocytic protein trafficking mediated by OsPIN3t
and clathrin to affect root growth
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 115
year: '2023'
...
---
_id: '13213'
abstract:
- lang: eng
text: The primary cell wall is a fundamental plant constituent that is flexible
but sufficiently rigid to support the plant cell shape. Although many studies
have demonstrated that reactive oxygen species (ROS) serve as important signaling
messengers to modify the cell wall structure and affect cellular growth, the regulatory
mechanism underlying the spatial-temporal regulation of ROS activity for cell
wall maintenance remains largely unclear. Here, we demonstrate the role of the
Arabidopsis (Arabidopsis thaliana) multicopper oxidase-like protein skewed 5 (SKU5)
and its homolog SKU5-similar 1 (SKS1) in root cell wall formation through modulating
ROS homeostasis. Loss of SKU5 and SKS1 function resulted in aberrant division
planes, protruding cell walls, ectopic deposition of iron, and reduced nicotinamide
adeninedinucleotide phosphate (NADPH) oxidase-dependent ROS overproduction in
the root epidermis–cortex and cortex–endodermis junctions. A decrease in ROS level
or inhibition of NADPH oxidase activity rescued the cell wall defects of sku5
sks1 double mutants. SKU5 and SKS1 proteins were activated by iron treatment,
and iron over-accumulated in the walls between the root epidermis and cortex cell
layers of sku5 sks1. The glycosylphosphatidylinositol-anchored motif was crucial
for membrane association and functionality of SKU5 and SKS1. Overall, our results
identified SKU5 and SKS1 as regulators of ROS at the cell surface for regulation
of cell wall structure and root cell growth.
acknowledgement: We thank Dong liu for offering iron staining technique; ZhiChang
Chen and Zhenbiao Yang for discussion; Dandan Zheng for earlier attempt; Liwen Jiang
and Dingquan Huang for initial tests of the TEM experiment; John C. Sedbrook for
a donation of sku5 and pSKU5::SKU5-GFP seeds; Catherine Perrot-Rechenmann and Ke
Zhou for the donation of sks1, sks2, and sku5 sks1 seeds; Zengyu Liu and Zhongquan
Lin for live-imaging microscopy assistance. We are grateful to Can Peng, and Xixia
Li for helping with sample preparation, and taking TEM images, at the Center for
Biological Imaging (CBI), Institute of Biophysics, Chinese Academy of Science.
article_processing_charge: No
article_type: original
author:
- first_name: C
full_name: Chen, C
last_name: Chen
- first_name: Y
full_name: Zhang, Y
last_name: Zhang
- first_name: J
full_name: Cai, J
last_name: Cai
- first_name: Y
full_name: Qiu, Y
last_name: Qiu
- first_name: L
full_name: Li, L
last_name: Li
- first_name: C
full_name: Gao, C
last_name: Gao
- first_name: Y
full_name: Gao, Y
last_name: Gao
- first_name: M
full_name: Ke, M
last_name: Ke
- first_name: S
full_name: Wu, S
last_name: Wu
- first_name: C
full_name: Wei, C
last_name: Wei
- first_name: J
full_name: Chen, J
last_name: Chen
- first_name: T
full_name: Xu, T
last_name: Xu
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: J
full_name: Wang, J
last_name: Wang
- first_name: R
full_name: Li, R
last_name: Li
- first_name: D
full_name: Chao, D
last_name: Chao
- first_name: B
full_name: Zhang, B
last_name: Zhang
- first_name: X
full_name: Chen, X
last_name: Chen
- first_name: Z
full_name: Gao, Z
last_name: Gao
citation:
ama: Chen C, Zhang Y, Cai J, et al. Multi-copper oxidases SKU5 and SKS1 coordinate
cell wall formation using apoplastic redox-based reactions in roots. Plant
Physiology. 2023;192(3):2243-2260. doi:10.1093/plphys/kiad207
apa: Chen, C., Zhang, Y., Cai, J., Qiu, Y., Li, L., Gao, C., … Gao, Z. (2023). Multi-copper
oxidases SKU5 and SKS1 coordinate cell wall formation using apoplastic redox-based
reactions in roots. Plant Physiology. American Society of Plant Biologists.
https://doi.org/10.1093/plphys/kiad207
chicago: Chen, C, Y Zhang, J Cai, Y Qiu, L Li, C Gao, Y Gao, et al. “Multi-Copper
Oxidases SKU5 and SKS1 Coordinate Cell Wall Formation Using Apoplastic Redox-Based
Reactions in Roots.” Plant Physiology. American Society of Plant Biologists,
2023. https://doi.org/10.1093/plphys/kiad207.
ieee: C. Chen et al., “Multi-copper oxidases SKU5 and SKS1 coordinate cell
wall formation using apoplastic redox-based reactions in roots,” Plant Physiology,
vol. 192, no. 3. American Society of Plant Biologists, pp. 2243–2260, 2023.
ista: Chen C, Zhang Y, Cai J, Qiu Y, Li L, Gao C, Gao Y, Ke M, Wu S, Wei C, Chen
J, Xu T, Friml J, Wang J, Li R, Chao D, Zhang B, Chen X, Gao Z. 2023. Multi-copper
oxidases SKU5 and SKS1 coordinate cell wall formation using apoplastic redox-based
reactions in roots. Plant Physiology. 192(3), 2243–2260.
mla: Chen, C., et al. “Multi-Copper Oxidases SKU5 and SKS1 Coordinate Cell Wall
Formation Using Apoplastic Redox-Based Reactions in Roots.” Plant Physiology,
vol. 192, no. 3, American Society of Plant Biologists, 2023, pp. 2243–60, doi:10.1093/plphys/kiad207.
short: C. Chen, Y. Zhang, J. Cai, Y. Qiu, L. Li, C. Gao, Y. Gao, M. Ke, S. Wu, C.
Wei, J. Chen, T. Xu, J. Friml, J. Wang, R. Li, D. Chao, B. Zhang, X. Chen, Z.
Gao, Plant Physiology 192 (2023) 2243–2260.
date_created: 2023-07-12T07:32:58Z
date_published: 2023-07-01T00:00:00Z
date_updated: 2023-08-02T06:27:55Z
day: '01'
ddc:
- '575'
department:
- _id: JiFr
doi: 10.1093/plphys/kiad207
external_id:
isi:
- '000971795800001'
pmid:
- '37010107'
file:
- access_level: open_access
checksum: 5492e1d18ac3eaf202633d210fa0fb75
content_type: application/pdf
creator: cchlebak
date_created: 2023-07-13T13:26:33Z
date_updated: 2023-07-13T13:26:33Z
file_id: '13220'
file_name: 2023_PlantPhys_Chen.pdf
file_size: 2076977
relation: main_file
success: 1
file_date_updated: 2023-07-13T13:26:33Z
has_accepted_license: '1'
intvolume: ' 192'
isi: 1
issue: '3'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
page: 2243-2260
pmid: 1
publication: Plant Physiology
publication_identifier:
eissn:
- 1532-2548
issn:
- 0032-0889
publication_status: published
publisher: American Society of Plant Biologists
quality_controlled: '1'
status: public
title: Multi-copper oxidases SKU5 and SKS1 coordinate cell wall formation using apoplastic
redox-based reactions in roots
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 192
year: '2023'
...
---
_id: '12543'
abstract:
- lang: eng
text: Treating sick group members is a hallmark of collective disease defence in
vertebrates and invertebrates alike. Despite substantial effects on pathogen fitness
and epidemiology, it is still largely unknown how pathogens react to the selection
pressure imposed by care intervention. Using social insects and pathogenic fungi,
we here performed a serial passage experiment in the presence or absence of colony
members, which provide social immunity by grooming off infectious spores from
exposed individuals. We found specific effects on pathogen diversity, virulence
and transmission. Under selection of social immunity, pathogens invested into
higher spore production, but spores were less virulent. Notably, they also elicited
a lower grooming response in colony members, compared with spores from the individual
host selection lines. Chemical spore analysis suggested that the spores from social
selection lines escaped the caregivers’ detection by containing lower levels of
ergosterol, a key fungal membrane component. Experimental application of chemically
pure ergosterol indeed induced sanitary grooming, supporting its role as a microbe-associated
cue triggering host social immunity against fungal pathogens. By reducing this
detection cue, pathogens were able to evade the otherwise very effective collective
disease defences of their social hosts.
acknowledged_ssus:
- _id: LifeSc
acknowledgement: We thank B. M. Steinwender, N. V. Meyling and J. Eilenberg for the
fungal strains; J. Anaya-Rojas for statistical advice; the Social Immunity team
at ISTA for ant collection and experimental help, in particular H. Leitner, and
the ISTA Lab Support Facility for general laboratory support; D. Ebert, H. Schulenburg
and J. Heinze for continued project discussion; and M. Sixt, R. Roemhild and the
Social Immunity team for comments on the manuscript. The study was funded by the
German Research Foundation (CR118/3-1) within the Framework of the Priority Program
SPP 1399, and the European Research Council (ERC) under the European Union’s Horizon
2020 Research and Innovation Programme (No. 771402; EPIDEMICSonCHIP), both to S.C.
article_processing_charge: No
article_type: original
author:
- first_name: Miriam
full_name: Stock, Miriam
id: 42462816-F248-11E8-B48F-1D18A9856A87
last_name: Stock
- first_name: Barbara
full_name: Milutinovic, Barbara
id: 2CDC32B8-F248-11E8-B48F-1D18A9856A87
last_name: Milutinovic
orcid: 0000-0002-8214-4758
- first_name: Michaela
full_name: Hönigsberger, Michaela
id: 953894f3-25bd-11ec-8556-f70a9d38ef60
last_name: Hönigsberger
- first_name: Anna V
full_name: Grasse, Anna V
id: 406F989C-F248-11E8-B48F-1D18A9856A87
last_name: Grasse
- first_name: Florian
full_name: Wiesenhofer, Florian
id: 39523C54-F248-11E8-B48F-1D18A9856A87
last_name: Wiesenhofer
- first_name: Niklas
full_name: Kampleitner, Niklas
id: 2AC57FAC-F248-11E8-B48F-1D18A9856A87
last_name: Kampleitner
- first_name: Madhumitha
full_name: Narasimhan, Madhumitha
id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
last_name: Narasimhan
orcid: 0000-0002-8600-0671
- first_name: Thomas
full_name: Schmitt, Thomas
last_name: Schmitt
- first_name: Sylvia
full_name: Cremer, Sylvia
id: 2F64EC8C-F248-11E8-B48F-1D18A9856A87
last_name: Cremer
orcid: 0000-0002-2193-3868
citation:
ama: Stock M, Milutinovic B, Hönigsberger M, et al. Pathogen evasion of social immunity.
Nature Ecology and Evolution. 2023;7:450-460. doi:10.1038/s41559-023-01981-6
apa: Stock, M., Milutinovic, B., Hönigsberger, M., Grasse, A. V., Wiesenhofer, F.,
Kampleitner, N., … Cremer, S. (2023). Pathogen evasion of social immunity. Nature
Ecology and Evolution. Springer Nature. https://doi.org/10.1038/s41559-023-01981-6
chicago: Stock, Miriam, Barbara Milutinovic, Michaela Hönigsberger, Anna V Grasse,
Florian Wiesenhofer, Niklas Kampleitner, Madhumitha Narasimhan, Thomas Schmitt,
and Sylvia Cremer. “Pathogen Evasion of Social Immunity.” Nature Ecology and
Evolution. Springer Nature, 2023. https://doi.org/10.1038/s41559-023-01981-6.
ieee: M. Stock et al., “Pathogen evasion of social immunity,” Nature Ecology
and Evolution, vol. 7. Springer Nature, pp. 450–460, 2023.
ista: Stock M, Milutinovic B, Hönigsberger M, Grasse AV, Wiesenhofer F, Kampleitner
N, Narasimhan M, Schmitt T, Cremer S. 2023. Pathogen evasion of social immunity.
Nature Ecology and Evolution. 7, 450–460.
mla: Stock, Miriam, et al. “Pathogen Evasion of Social Immunity.” Nature Ecology
and Evolution, vol. 7, Springer Nature, 2023, pp. 450–60, doi:10.1038/s41559-023-01981-6.
short: M. Stock, B. Milutinovic, M. Hönigsberger, A.V. Grasse, F. Wiesenhofer, N.
Kampleitner, M. Narasimhan, T. Schmitt, S. Cremer, Nature Ecology and Evolution
7 (2023) 450–460.
date_created: 2023-02-12T23:00:59Z
date_published: 2023-03-01T00:00:00Z
date_updated: 2023-08-16T11:55:48Z
day: '01'
ddc:
- '570'
department:
- _id: SyCr
- _id: LifeSc
- _id: JiFr
doi: 10.1038/s41559-023-01981-6
ec_funded: 1
external_id:
isi:
- '000924572800001'
pmid:
- '36732670'
file:
- access_level: open_access
checksum: 8244f4650a0e7aeea488d1bcd4a31702
content_type: application/pdf
creator: dernst
date_created: 2023-08-16T11:54:59Z
date_updated: 2023-08-16T11:54:59Z
file_id: '14069'
file_name: 2023_NatureEcoEvo_Stock.pdf
file_size: 1600499
relation: main_file
success: 1
file_date_updated: 2023-08-16T11:54:59Z
has_accepted_license: '1'
intvolume: ' 7'
isi: 1
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
page: 450-460
pmid: 1
project:
- _id: 2649B4DE-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '771402'
name: Epidemics in ant societies on a chip
- _id: 25DAF0B2-B435-11E9-9278-68D0E5697425
grant_number: CR-118/3-1
name: Host-Parasite Coevolution
publication: Nature Ecology and Evolution
publication_identifier:
eissn:
- 2397-334X
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on ISTA website
relation: press_release
url: https://ista.ac.at/en/news/how-sneaky-germs-hide-from-ants/
scopus_import: '1'
status: public
title: Pathogen evasion of social immunity
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 7
year: '2023'
...
---
_id: '14313'
abstract:
- lang: eng
text: To respond to auxin, the chief orchestrator of their multicellularity, plants
evolved multiple receptor systems and signal transduction cascades. Despite decades
of research, however, we are still lacking a satisfactory synthesis of various
auxin signaling mechanisms. The chief discrepancy and historical controversy of
the field is that of rapid and slow auxin effects on plant physiology and development.
How is it possible that ions begin to trickle across the plasma membrane as soon
as auxin enters the cell, even though the best-characterized transcriptional auxin
pathway can take effect only after tens of minutes? Recently, unexpected progress
has been made in understanding this and other unknowns of auxin signaling. We
provide a perspective on these exciting developments and concepts whose general
applicability might have ramifications beyond auxin signaling.
acknowledgement: The opening quote is not intended to reflect any political views
of the authors. The authors by no means endorse the rhetoric of Donald Rumsfeld
or the 2003 invasion of Iraq by the United States. Nevertheless, Rumsfeld's quote
led to both public and academic debates on the concept of known and unknown unknowns,
which can be applied to the recent unexpected developments in the auxin signaling
field. We thank Linlin Qi and Huihuang Chen for their suggestions on figure presentation
and inspiring discussions of TIR1/AFB signaling. Finally, we thank Aroosa Hussain
for discussion of Greek mythology.
article_number: '102443'
article_processing_charge: No
article_type: review
author:
- first_name: Lukas
full_name: Fiedler, Lukas
id: 7c417475-8972-11ed-ae7b-8b674ca26986
last_name: Fiedler
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Fiedler L, Friml J. Rapid auxin signaling: Unknowns old and new. Current
Opinion in Plant Biology. 2023;75(10). doi:10.1016/j.pbi.2023.102443'
apa: 'Fiedler, L., & Friml, J. (2023). Rapid auxin signaling: Unknowns old and
new. Current Opinion in Plant Biology. Elsevier. https://doi.org/10.1016/j.pbi.2023.102443'
chicago: 'Fiedler, Lukas, and Jiří Friml. “Rapid Auxin Signaling: Unknowns Old and
New.” Current Opinion in Plant Biology. Elsevier, 2023. https://doi.org/10.1016/j.pbi.2023.102443.'
ieee: 'L. Fiedler and J. Friml, “Rapid auxin signaling: Unknowns old and new,” Current
Opinion in Plant Biology, vol. 75, no. 10. Elsevier, 2023.'
ista: 'Fiedler L, Friml J. 2023. Rapid auxin signaling: Unknowns old and new. Current
Opinion in Plant Biology. 75(10), 102443.'
mla: 'Fiedler, Lukas, and Jiří Friml. “Rapid Auxin Signaling: Unknowns Old and New.”
Current Opinion in Plant Biology, vol. 75, no. 10, 102443, Elsevier, 2023,
doi:10.1016/j.pbi.2023.102443.'
short: L. Fiedler, J. Friml, Current Opinion in Plant Biology 75 (2023).
date_created: 2023-09-10T22:01:11Z
date_published: 2023-10-01T00:00:00Z
date_updated: 2023-11-07T08:17:13Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.pbi.2023.102443
external_id:
pmid:
- '37666097'
file:
- access_level: open_access
checksum: 1c476c3414d2dfb0c85db0cb6cfd8a28
content_type: application/pdf
creator: amally
date_created: 2023-11-02T17:03:20Z
date_updated: 2023-11-02T17:03:20Z
file_id: '14482'
file_name: Fiedler CurrOpinOlantBiol 2023_revised.pdf
file_size: 737872
relation: main_file
success: 1
file_date_updated: 2023-11-02T17:03:20Z
has_accepted_license: '1'
intvolume: ' 75'
issue: '10'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Submitted Version
pmid: 1
publication: Current Opinion in Plant Biology
publication_identifier:
issn:
- 1369-5266
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Rapid auxin signaling: Unknowns old and new'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 75
year: '2023'
...
---
_id: '14591'
abstract:
- lang: eng
text: Clathrin-mediated endocytosis (CME) is vital for the regulation of plant growth
and development by controlling plasma membrane protein composition and cargo uptake.
CME relies on the precise recruitment of regulators for vesicle maturation and
release. Homologues of components of mammalian vesicle scission are strong candidates
to be part of the scissin machinery in plants, but the precise roles of these
proteins in this process is not fully understood. Here, we characterised the roles
of Plant Dynamin-Related Proteins 2 (DRP2s) and SH3-domain containing protein
2 (SH3P2), the plant homologue to Dynamins’ recruiters, like Endophilin and Amphiphysin,
in the CME by combining high-resolution imaging of endocytic events in vivo and
characterisation of the purified proteins in vitro. Although DRP2s and SH3P2 arrive
similarly late during CME and physically interact, genetic analysis of the Dsh3p1,2,3
triple-mutant and complementation assays with non-SH3P2-interacting DRP2 variants
suggests that SH3P2 does not directly recruit DRP2s to the site of endocytosis.
These observations imply that despite the presence of many well-conserved endocytic
components, plants have acquired a distinct mechanism for CME. One Sentence Summary
In contrast to predictions based on mammalian systems, plant Dynamin-related proteins
2 are recruited to the site of Clathrin-mediated endocytosis independently of
BAR-SH3 proteins.
acknowledged_ssus:
- _id: EM-Fac
- _id: LifeSc
- _id: Bio
article_processing_charge: No
author:
- first_name: Nataliia
full_name: Gnyliukh, Nataliia
id: 390C1120-F248-11E8-B48F-1D18A9856A87
last_name: Gnyliukh
orcid: 0000-0002-2198-0509
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Marie-Kristin
full_name: Nagel, Marie-Kristin
last_name: Nagel
- first_name: Aline
full_name: Monzer, Aline
id: 2DB5D88C-D7B3-11E9-B8FD-7907E6697425
last_name: Monzer
- first_name: Annamaria
full_name: Hlavata, Annamaria
id: 36062FEC-F248-11E8-B48F-1D18A9856A87
last_name: Hlavata
- first_name: Erika
full_name: Isono, Erika
last_name: Isono
- first_name: Martin
full_name: Loose, Martin
id: 462D4284-F248-11E8-B48F-1D18A9856A87
last_name: Loose
orcid: 0000-0001-7309-9724
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Gnyliukh N, Johnson AJ, Nagel M-K, et al. Role of dynamin-related proteins
2 and SH3P2 in clathrin-mediated endocytosis in plants. bioRxiv. doi:10.1101/2023.10.09.561523
apa: Gnyliukh, N., Johnson, A. J., Nagel, M.-K., Monzer, A., Hlavata, A., Isono,
E., … Friml, J. (n.d.). Role of dynamin-related proteins 2 and SH3P2 in clathrin-mediated
endocytosis in plants. bioRxiv. https://doi.org/10.1101/2023.10.09.561523
chicago: Gnyliukh, Nataliia, Alexander J Johnson, Marie-Kristin Nagel, Aline Monzer,
Annamaria Hlavata, Erika Isono, Martin Loose, and Jiří Friml. “Role of Dynamin-Related
Proteins 2 and SH3P2 in Clathrin-Mediated Endocytosis in Plants.” BioRxiv,
n.d. https://doi.org/10.1101/2023.10.09.561523.
ieee: N. Gnyliukh et al., “Role of dynamin-related proteins 2 and SH3P2 in
clathrin-mediated endocytosis in plants,” bioRxiv. .
ista: Gnyliukh N, Johnson AJ, Nagel M-K, Monzer A, Hlavata A, Isono E, Loose M,
Friml J. Role of dynamin-related proteins 2 and SH3P2 in clathrin-mediated endocytosis
in plants. bioRxiv, 10.1101/2023.10.09.561523.
mla: Gnyliukh, Nataliia, et al. “Role of Dynamin-Related Proteins 2 and SH3P2 in
Clathrin-Mediated Endocytosis in Plants.” BioRxiv, doi:10.1101/2023.10.09.561523.
short: N. Gnyliukh, A.J. Johnson, M.-K. Nagel, A. Monzer, A. Hlavata, E. Isono,
M. Loose, J. Friml, BioRxiv (n.d.).
date_created: 2023-11-22T10:17:49Z
date_published: 2023-10-10T00:00:00Z
date_updated: 2023-12-01T13:51:06Z
day: '10'
department:
- _id: JiFr
- _id: MaLo
- _id: CaBe
doi: 10.1101/2023.10.09.561523
ec_funded: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.biorxiv.org/content/10.1101/2023.10.09.561523v2
month: '10'
oa: 1
oa_version: Preprint
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
publication: bioRxiv
publication_status: submitted
related_material:
record:
- id: '14510'
relation: dissertation_contains
status: public
status: public
title: Role of dynamin-related proteins 2 and SH3P2 in clathrin-mediated endocytosis
in plants
type: preprint
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
year: '2023'
...
---
_id: '14339'
abstract:
- lang: eng
text: Lateral roots are typically maintained at non-vertical angles with respect
to gravity. These gravitropic setpoint angles are intriguing because their maintenance
requires that roots are able to effect growth response both with and against the
gravity vector, a phenomenon previously attributed to gravitropism acting against
an antigravitropic offset mechanism. Here we show how the components mediating
gravitropism in the vertical primary root—PINs and phosphatases acting upon them—are
reconfigured in their regulation such that lateral root growth at a range of angles
can be maintained. We show that the ability of Arabidopsis lateral roots to bend
both downward and upward requires the generation of auxin asymmetries and is driven
by angle-dependent variation in downward gravitropic auxin flux acting against
angle-independent upward, antigravitropic flux. Further, we demonstrate a symmetry
in auxin distribution in lateral roots at gravitropic setpoint angle that can
be traced back to a net, balanced polarization of PIN3 and PIN7 auxin transporters
in the columella. These auxin fluxes are shifted by altering PIN protein phosphoregulation
in the columella, either by introducing PIN3 phosphovariant versions or via manipulation
of levels of the phosphatase subunit PP2A/RCN1. Finally, we show that auxin, in
addition to driving lateral root directional growth, acts within the lateral root
columella to induce more vertical growth by increasing RCN1 levels, causing a
downward shift in PIN3 localization, thereby diminishing the magnitude of the
upward, antigravitropic auxin flux.
acknowledgement: We thank D. Weijers, C. Schwechheimer and R. Offringa for generous
sharing of published and unpublished materials and P. Masson for advice on the use
of the ARL2 promoter. We are grateful to M. Del Bianco and O. Leyser for critical
reading of the manuscript. This work was supported by the BBSRC (grants BB/N010124/1
and BB/R000859/1 to S.K.), the Gatsby Charitable Foundation and the Leverhulme Trust
(RPG-2018-137 to S.K.).
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: S
full_name: Roychoudhry, S
last_name: Roychoudhry
- first_name: K
full_name: Sageman-Furnas, K
last_name: Sageman-Furnas
- first_name: C
full_name: Wolverton, C
last_name: Wolverton
- first_name: Peter
full_name: Grones, Peter
id: 399876EC-F248-11E8-B48F-1D18A9856A87
last_name: Grones
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Gergely
full_name: Molnar, Gergely
id: 34F1AF46-F248-11E8-B48F-1D18A9856A87
last_name: Molnar
- first_name: M
full_name: De Angelis, M
last_name: De Angelis
- first_name: HL
full_name: Goodman, HL
last_name: Goodman
- first_name: N
full_name: Capstaff, N
last_name: Capstaff
- first_name: Lloyd
full_name: JPB, Lloyd
last_name: JPB
- first_name: J
full_name: Mullen, J
last_name: Mullen
- first_name: R
full_name: Hangarter, R
last_name: Hangarter
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: S
full_name: Kepinski, S
last_name: Kepinski
citation:
ama: Roychoudhry S, Sageman-Furnas K, Wolverton C, et al. Antigravitropic PIN polarization
maintains non-vertical growth in lateral roots. Nature Plants. 2023;9:1500-1513.
doi:10.1038/s41477-023-01478-x
apa: Roychoudhry, S., Sageman-Furnas, K., Wolverton, C., Grones, P., Tan, S., Molnar,
G., … Kepinski, S. (2023). Antigravitropic PIN polarization maintains non-vertical
growth in lateral roots. Nature Plants. Springer Nature. https://doi.org/10.1038/s41477-023-01478-x
chicago: Roychoudhry, S, K Sageman-Furnas, C Wolverton, Peter Grones, Shutang Tan,
Gergely Molnar, M De Angelis, et al. “Antigravitropic PIN Polarization Maintains
Non-Vertical Growth in Lateral Roots.” Nature Plants. Springer Nature,
2023. https://doi.org/10.1038/s41477-023-01478-x.
ieee: S. Roychoudhry et al., “Antigravitropic PIN polarization maintains
non-vertical growth in lateral roots,” Nature Plants, vol. 9. Springer
Nature, pp. 1500–1513, 2023.
ista: Roychoudhry S, Sageman-Furnas K, Wolverton C, Grones P, Tan S, Molnar G, De
Angelis M, Goodman H, Capstaff N, JPB L, Mullen J, Hangarter R, Friml J, Kepinski
S. 2023. Antigravitropic PIN polarization maintains non-vertical growth in lateral
roots. Nature Plants. 9, 1500–1513.
mla: Roychoudhry, S., et al. “Antigravitropic PIN Polarization Maintains Non-Vertical
Growth in Lateral Roots.” Nature Plants, vol. 9, Springer Nature, 2023,
pp. 1500–13, doi:10.1038/s41477-023-01478-x.
short: S. Roychoudhry, K. Sageman-Furnas, C. Wolverton, P. Grones, S. Tan, G. Molnar,
M. De Angelis, H. Goodman, N. Capstaff, L. JPB, J. Mullen, R. Hangarter, J. Friml,
S. Kepinski, Nature Plants 9 (2023) 1500–1513.
date_created: 2023-09-15T09:56:01Z
date_published: 2023-09-01T00:00:00Z
date_updated: 2023-12-13T12:23:49Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41477-023-01478-x
external_id:
isi:
- '001069238800014'
pmid:
- '37666965'
file:
- access_level: open_access
checksum: 3d6d5d5abb937c14a5f6f0afba3b8624
content_type: application/pdf
creator: dernst
date_created: 2023-09-20T10:51:31Z
date_updated: 2023-09-20T10:51:31Z
file_id: '14351'
file_name: 2023_NaturePlants_Roychoudhry.pdf
file_size: 9647103
relation: main_file
success: 1
file_date_updated: 2023-09-20T10:51:31Z
has_accepted_license: '1'
intvolume: ' 9'
isi: 1
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
page: 1500-1513
pmid: 1
publication: Nature Plants
publication_identifier:
issn:
- 2055-0278
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
status: public
title: Antigravitropic PIN polarization maintains non-vertical growth in lateral roots
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 9
year: '2023'
...
---
_id: '14447'
abstract:
- lang: eng
text: "Auxin belongs among major phytohormones and governs multiple aspects of plant
growth and development. The establishment of auxin concentration gradients, determines,
among other processes, plant organ positioning and growth responses to environmental
stimuli.\r\nHerein we report the synthesis of new NBD- or DNS-labelled IAA derivatives
and the elucidation of their biological activity, fluorescence properties and
subcellular accumulation patterns in planta. These novel compounds did not show
auxin-like activity, but instead antagonized physiological auxin effects. The
DNS-labelled derivatives FL5 and FL6 showed strong anti-auxin activity in roots
and hypocotyls, which also occurred at the level of gene transcription as confirmed
by quantitative PCR analysis. The auxin antagonism of our derivatives was further
demonstrated in vitro using an SPR-based binding assay. The NBD-labelled compound
FL4 with the best fluorescence properties proved to be unsuitable to study auxin
accumulation patterns in planta. On the other hand, the strongest anti-auxin activity
possessing compounds FL5 and FL6 could be useful to study binding mechanisms to
auxin receptors and for manipulations of auxin-regulated processes."
acknowledgement: The authors would like to thank Karolína Kubiasová and Iñigo Saiz-Fernández
for valuable scientific discussions. Open access publishing supported by the National
Technical Library in Prague. This work was supported by the Palacký University Olomouc
Young Researcher Grant Competition (JG_2020_002), by the Internal Grant Agency of
Palacký University Olomouc (IGA_PrF_2023_016, IGA_PrF_2023_031), by the Ministry
of Education, Youth and Sports of the Czech Republic through the European Regional
Development Fund-Project Plants as a tool for sustainable global development (CZ.02.1.01/0.0/0.0/16_019/0000827)
and the project Support of mobility at Palacký University Olomouc II. (CZ.02.2.69/0.0/0.0/18_053/0016919).
The Biacore T200 SPR instrument was provided by the WISB Research Technology Facility
within the School of Life Sciences, University of Warwick.
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Kristýna
full_name: Bieleszová, Kristýna
last_name: Bieleszová
- first_name: Pavel
full_name: Hladík, Pavel
last_name: Hladík
- first_name: Martin
full_name: Kubala, Martin
last_name: Kubala
- first_name: Richard
full_name: Napier, Richard
last_name: Napier
- first_name: Federica
full_name: Brunoni, Federica
last_name: Brunoni
- first_name: Zuzana
full_name: Gelová, Zuzana
id: 0AE74790-0E0B-11E9-ABC7-1ACFE5697425
last_name: Gelová
orcid: 0000-0003-4783-1752
- first_name: Lukas
full_name: Fiedler, Lukas
id: 7c417475-8972-11ed-ae7b-8b674ca26986
last_name: Fiedler
- first_name: Ivan
full_name: Kulich, Ivan
id: 57a1567c-8314-11eb-9063-c9ddc3451a54
last_name: Kulich
- first_name: Miroslav
full_name: Strnad, Miroslav
last_name: Strnad
- first_name: Karel
full_name: Doležal, Karel
last_name: Doležal
- first_name: Ondřej
full_name: Novák, Ondřej
last_name: Novák
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Asta
full_name: Žukauskaitė, Asta
last_name: Žukauskaitė
citation:
ama: 'Bieleszová K, Hladík P, Kubala M, et al. New fluorescent auxin derivatives:
anti-auxin activity and accumulation patterns in Arabidopsis thaliana. Plant
Growth Regulation. 2023. doi:10.1007/s10725-023-01083-0'
apa: 'Bieleszová, K., Hladík, P., Kubala, M., Napier, R., Brunoni, F., Gelová, Z.,
… Žukauskaitė, A. (2023). New fluorescent auxin derivatives: anti-auxin activity
and accumulation patterns in Arabidopsis thaliana. Plant Growth Regulation.
Springer Nature. https://doi.org/10.1007/s10725-023-01083-0'
chicago: 'Bieleszová, Kristýna, Pavel Hladík, Martin Kubala, Richard Napier, Federica
Brunoni, Zuzana Gelová, Lukas Fiedler, et al. “New Fluorescent Auxin Derivatives:
Anti-Auxin Activity and Accumulation Patterns in Arabidopsis Thaliana.” Plant
Growth Regulation. Springer Nature, 2023. https://doi.org/10.1007/s10725-023-01083-0.'
ieee: 'K. Bieleszová et al., “New fluorescent auxin derivatives: anti-auxin
activity and accumulation patterns in Arabidopsis thaliana,” Plant Growth Regulation.
Springer Nature, 2023.'
ista: 'Bieleszová K, Hladík P, Kubala M, Napier R, Brunoni F, Gelová Z, Fiedler
L, Kulich I, Strnad M, Doležal K, Novák O, Friml J, Žukauskaitė A. 2023. New fluorescent
auxin derivatives: anti-auxin activity and accumulation patterns in Arabidopsis
thaliana. Plant Growth Regulation.'
mla: 'Bieleszová, Kristýna, et al. “New Fluorescent Auxin Derivatives: Anti-Auxin
Activity and Accumulation Patterns in Arabidopsis Thaliana.” Plant Growth Regulation,
Springer Nature, 2023, doi:10.1007/s10725-023-01083-0.'
short: K. Bieleszová, P. Hladík, M. Kubala, R. Napier, F. Brunoni, Z. Gelová, L.
Fiedler, I. Kulich, M. Strnad, K. Doležal, O. Novák, J. Friml, A. Žukauskaitė,
Plant Growth Regulation (2023).
date_created: 2023-10-22T22:01:15Z
date_published: 2023-10-13T00:00:00Z
date_updated: 2023-12-13T13:08:25Z
day: '13'
department:
- _id: JiFr
doi: 10.1007/s10725-023-01083-0
external_id:
isi:
- '001084334300001'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1007/s10725-023-01083-0
month: '10'
oa: 1
oa_version: Published Version
publication: Plant Growth Regulation
publication_identifier:
eissn:
- 1573-5087
issn:
- 0167-6903
publication_status: epub_ahead
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'New fluorescent auxin derivatives: anti-auxin activity and accumulation patterns
in Arabidopsis thaliana'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2023'
...
---
_id: '14709'
abstract:
- lang: eng
text: Amid the delays due to the global pandemic, in early October 2022, the auxin
community gathered in the idyllic peninsula of Cavtat, Croatia. More than 170
scientists from across the world converged to discuss the latest advancements
in fundamental and applied research in the field. The topics, from signalling
and transport to plant architecture and response to the environment, show how
auxin research must bridge from the molecular realm to macroscopic developmental
responses. This is mirrored in this collection of reviews, contributed by participants
of the Auxin 2022 meeting.
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Marta
full_name: Del Bianco, Marta
last_name: Del Bianco
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Lucia
full_name: Strader, Lucia
last_name: Strader
- first_name: Stefan
full_name: Kepinski, Stefan
last_name: Kepinski
citation:
ama: 'Del Bianco M, Friml J, Strader L, Kepinski S. Auxin research: Creating tools
for a greener future. Journal of Experimental Botany. 2023;74(22):6889-6892.
doi:10.1093/jxb/erad420'
apa: 'Del Bianco, M., Friml, J., Strader, L., & Kepinski, S. (2023). Auxin research:
Creating tools for a greener future. Journal of Experimental Botany. Oxford
University Press. https://doi.org/10.1093/jxb/erad420'
chicago: 'Del Bianco, Marta, Jiří Friml, Lucia Strader, and Stefan Kepinski. “Auxin
Research: Creating Tools for a Greener Future.” Journal of Experimental Botany.
Oxford University Press, 2023. https://doi.org/10.1093/jxb/erad420.'
ieee: 'M. Del Bianco, J. Friml, L. Strader, and S. Kepinski, “Auxin research: Creating
tools for a greener future,” Journal of Experimental Botany, vol. 74, no.
22. Oxford University Press, pp. 6889–6892, 2023.'
ista: 'Del Bianco M, Friml J, Strader L, Kepinski S. 2023. Auxin research: Creating
tools for a greener future. Journal of Experimental Botany. 74(22), 6889–6892.'
mla: 'Del Bianco, Marta, et al. “Auxin Research: Creating Tools for a Greener Future.”
Journal of Experimental Botany, vol. 74, no. 22, Oxford University Press,
2023, pp. 6889–92, doi:10.1093/jxb/erad420.'
short: M. Del Bianco, J. Friml, L. Strader, S. Kepinski, Journal of Experimental
Botany 74 (2023) 6889–6892.
date_created: 2023-12-24T23:00:53Z
date_published: 2023-12-01T00:00:00Z
date_updated: 2024-01-02T09:29:24Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1093/jxb/erad420
external_id:
pmid:
- '38038239'
file:
- access_level: open_access
checksum: f66fb960fd791dea53fd0e087f2fbbe8
content_type: application/pdf
creator: dernst
date_created: 2024-01-02T09:23:57Z
date_updated: 2024-01-02T09:23:57Z
file_id: '14724'
file_name: 2023_JourExperimentalBotany_DelBianco.pdf
file_size: 425194
relation: main_file
success: 1
file_date_updated: 2024-01-02T09:23:57Z
has_accepted_license: '1'
intvolume: ' 74'
issue: '22'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
page: 6889-6892
pmid: 1
publication: Journal of Experimental Botany
publication_identifier:
eissn:
- 1460-2431
issn:
- 0022-0957
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Auxin research: Creating tools for a greener future'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 74
year: '2023'
...
---
_id: '14776'
abstract:
- lang: eng
text: Soluble chaperones residing in the endoplasmic reticulum (ER) play vitally
important roles in folding and quality control of newly synthesized proteins that
transiently pass through the ER en route to their final destinations. These soluble
residents of the ER are themselves endowed with an ER retrieval signal that enables
the cell to bring the escaped residents back from the Golgi. Here, by using purified
proteins, we showed that Nicotiana tabacum phytaspase, a plant aspartate-specific
protease, introduces two breaks at the C-terminus of the N. tabacum ER resident
calreticulin-3. These cleavages resulted in removal of either a dipeptide or a
hexapeptide from the C-terminus of calreticulin-3 encompassing part or all of
the ER retrieval signal. Consistently, expression of the calreticulin-3 derivative
mimicking the phytaspase cleavage product in Nicotiana benthamiana cells demonstrated
loss of the ER accumulation of the protein. Notably, upon its escape from the
ER, calreticulin-3 was further processed by an unknown protease(s) to generate
the free N-terminal (N) domain of calreticulin-3, which was ultimately secreted
into the apoplast. Our study thus identified a specific proteolytic enzyme capable
of precise detachment of the ER retrieval signal from a plant ER resident protein,
with implications for the further fate of the escaped resident.
acknowledgement: "We thank C.U.T. Hellen for critically reading the manuscript. The
MALDI MS facility and CLSM became available to us in the framework of Moscow State
University Development Programs PNG 5.13 and PNR 5.13.\r\nThis work was funded by
the Russian Science Foundation, grant numbers 19-14-00010 and 22-14-00071."
article_number: '16527'
article_processing_charge: Yes
article_type: original
author:
- first_name: Anastasiia
full_name: Teplova, Anastasiia
id: e3736151-106c-11ec-b916-c2558e2762c6
last_name: Teplova
- first_name: Artemii A.
full_name: Pigidanov, Artemii A.
last_name: Pigidanov
- first_name: Marina V.
full_name: Serebryakova, Marina V.
last_name: Serebryakova
- first_name: Sergei A.
full_name: Golyshev, Sergei A.
last_name: Golyshev
- first_name: Raisa A.
full_name: Galiullina, Raisa A.
last_name: Galiullina
- first_name: Nina V.
full_name: Chichkova, Nina V.
last_name: Chichkova
- first_name: Andrey B.
full_name: Vartapetian, Andrey B.
last_name: Vartapetian
citation:
ama: Teplova A, Pigidanov AA, Serebryakova MV, et al. Phytaspase Is capable of detaching
the endoplasmic reticulum retrieval signal from tobacco calreticulin-3. International
Journal of Molecular Sciences. 2023;24(22). doi:10.3390/ijms242216527
apa: Teplova, A., Pigidanov, A. A., Serebryakova, M. V., Golyshev, S. A., Galiullina,
R. A., Chichkova, N. V., & Vartapetian, A. B. (2023). Phytaspase Is capable
of detaching the endoplasmic reticulum retrieval signal from tobacco calreticulin-3.
International Journal of Molecular Sciences. MDPI. https://doi.org/10.3390/ijms242216527
chicago: Teplova, Anastasiia, Artemii A. Pigidanov, Marina V. Serebryakova, Sergei
A. Golyshev, Raisa A. Galiullina, Nina V. Chichkova, and Andrey B. Vartapetian.
“Phytaspase Is Capable of Detaching the Endoplasmic Reticulum Retrieval Signal
from Tobacco Calreticulin-3.” International Journal of Molecular Sciences.
MDPI, 2023. https://doi.org/10.3390/ijms242216527.
ieee: A. Teplova et al., “Phytaspase Is capable of detaching the endoplasmic
reticulum retrieval signal from tobacco calreticulin-3,” International Journal
of Molecular Sciences, vol. 24, no. 22. MDPI, 2023.
ista: Teplova A, Pigidanov AA, Serebryakova MV, Golyshev SA, Galiullina RA, Chichkova
NV, Vartapetian AB. 2023. Phytaspase Is capable of detaching the endoplasmic reticulum
retrieval signal from tobacco calreticulin-3. International Journal of Molecular
Sciences. 24(22), 16527.
mla: Teplova, Anastasiia, et al. “Phytaspase Is Capable of Detaching the Endoplasmic
Reticulum Retrieval Signal from Tobacco Calreticulin-3.” International Journal
of Molecular Sciences, vol. 24, no. 22, 16527, MDPI, 2023, doi:10.3390/ijms242216527.
short: A. Teplova, A.A. Pigidanov, M.V. Serebryakova, S.A. Golyshev, R.A. Galiullina,
N.V. Chichkova, A.B. Vartapetian, International Journal of Molecular Sciences
24 (2023).
date_created: 2024-01-10T09:24:35Z
date_published: 2023-11-01T00:00:00Z
date_updated: 2024-01-10T13:41:10Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.3390/ijms242216527
external_id:
isi:
- '001113792600001'
pmid:
- '38003717'
file:
- access_level: open_access
checksum: 4df7d206ba022b7f54eff1f0aec1659a
content_type: application/pdf
creator: dernst
date_created: 2024-01-10T13:39:42Z
date_updated: 2024-01-10T13:39:42Z
file_id: '14791'
file_name: 2023_IJMS_Teplova.pdf
file_size: 2637784
relation: main_file
success: 1
file_date_updated: 2024-01-10T13:39:42Z
has_accepted_license: '1'
intvolume: ' 24'
isi: 1
issue: '22'
keyword:
- Inorganic Chemistry
- Organic Chemistry
- Physical and Theoretical Chemistry
- Computer Science Applications
- Spectroscopy
- Molecular Biology
- General Medicine
- Catalysis
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
publication: International Journal of Molecular Sciences
publication_identifier:
issn:
- 1422-0067
publication_status: published
publisher: MDPI
quality_controlled: '1'
status: public
title: Phytaspase Is capable of detaching the endoplasmic reticulum retrieval signal
from tobacco calreticulin-3
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 24
year: '2023'
...
---
_id: '13212'
abstract:
- lang: eng
text: Auxin is the major plant hormone regulating growth and development (Friml,
2022). Forward genetic approaches in the model plant Arabidopsis thaliana have
identified major components of auxin signalling and established the canonical
mechanism mediating transcriptional and thus developmental reprogramming. In this
textbook view, TRANSPORT INHIBITOR RESPONSE 1 (TIR1)/AUXIN-SIGNALING F-BOX (AFBs)
are auxin receptors, which act as F-box subunits determining the substrate specificity
of the Skp1-Cullin1-F box protein (SCF) type E3 ubiquitin ligase complex. Auxin
acts as a “molecular glue” increasing the affinity between TIR1/AFBs and the Aux/IAA
repressors. Subsequently, Aux/IAAs are ubiquitinated and degraded, thus releasing
auxin transcription factors from their repression making them free to mediate
transcription of auxin response genes (Yu et al., 2022). Nonetheless, accumulating
evidence suggests existence of rapid, non-transcriptional responses downstream
of TIR1/AFBs such as auxin-induced cytosolic calcium (Ca2+) transients, plasma
membrane depolarization and apoplast alkalinisation, all converging on the process
of root growth inhibition and root gravitropism (Li et al., 2022). Particularly,
these rapid responses are mostly contributed by predominantly cytosolic AFB1,
while the long-term growth responses are mediated by mainly nuclear TIR1 and AFB2-AFB5
(Li et al., 2021; Prigge et al., 2020; Serre et al., 2021). How AFB1 conducts
auxin-triggered rapid responses and how it is different from TIR1 and AFB2-AFB5
remains elusive. Here, we compare the roles of TIR1 and AFB1 in transcriptional
and rapid responses by modulating their subcellular localization in Arabidopsis
and by testing their ability to mediate transcriptional responses when part of
the minimal auxin circuit reconstituted in yeast.
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
acknowledgement: We thank all the authors for sharing the published materials. This
research was supported by the Lab Support Facility and the Imaging and Optics Facility
of ISTA. We thank Lukáš Fiedler (ISTA) for critical reading of the manuscript. This
project was funded by the European Research Council Advanced Grant (ETAP-742985).
article_processing_charge: Yes (via OA deal)
article_type: letter_note
author:
- first_name: Huihuang
full_name: Chen, Huihuang
id: 83c96512-15b2-11ec-abd3-b7eede36184f
last_name: Chen
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Minxia
full_name: Zou, Minxia
id: 5c243f41-03f3-11ec-841c-96faf48a7ef9
last_name: Zou
- first_name: Linlin
full_name: Qi, Linlin
id: 44B04502-A9ED-11E9-B6FC-583AE6697425
last_name: Qi
orcid: 0000-0001-5187-8401
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Chen H, Li L, Zou M, Qi L, Friml J. Distinct functions of TIR1 and AFB1 receptors
in auxin signalling. Molecular Plant. 2023;16(7):1117-1119. doi:10.1016/j.molp.2023.06.007
apa: Chen, H., Li, L., Zou, M., Qi, L., & Friml, J. (2023). Distinct functions
of TIR1 and AFB1 receptors in auxin signalling. Molecular Plant. Elsevier
. https://doi.org/10.1016/j.molp.2023.06.007
chicago: Chen, Huihuang, Lanxin Li, Minxia Zou, Linlin Qi, and Jiří Friml. “Distinct
Functions of TIR1 and AFB1 Receptors in Auxin Signalling.” Molecular Plant.
Elsevier , 2023. https://doi.org/10.1016/j.molp.2023.06.007.
ieee: H. Chen, L. Li, M. Zou, L. Qi, and J. Friml, “Distinct functions of TIR1 and
AFB1 receptors in auxin signalling.,” Molecular Plant, vol. 16, no. 7.
Elsevier , pp. 1117–1119, 2023.
ista: Chen H, Li L, Zou M, Qi L, Friml J. 2023. Distinct functions of TIR1 and AFB1
receptors in auxin signalling. Molecular Plant. 16(7), 1117–1119.
mla: Chen, Huihuang, et al. “Distinct Functions of TIR1 and AFB1 Receptors in Auxin
Signalling.” Molecular Plant, vol. 16, no. 7, Elsevier , 2023, pp. 1117–19,
doi:10.1016/j.molp.2023.06.007.
short: H. Chen, L. Li, M. Zou, L. Qi, J. Friml, Molecular Plant 16 (2023) 1117–1119.
date_created: 2023-07-12T07:32:46Z
date_published: 2023-07-01T00:00:00Z
date_updated: 2024-01-29T10:38:57Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.molp.2023.06.007
ec_funded: 1
external_id:
isi:
- '001044410900001'
pmid:
- '37393433'
file:
- access_level: open_access
checksum: 6012b7e4a2f680ee6c1f84001e2b945f
content_type: application/pdf
creator: dernst
date_created: 2024-01-29T10:37:05Z
date_updated: 2024-01-29T10:37:05Z
file_id: '14894'
file_name: 2023_MolecularPlant_Chen.pdf
file_size: 1000871
relation: main_file
success: 1
file_date_updated: 2024-01-29T10:37:05Z
has_accepted_license: '1'
intvolume: ' 16'
isi: 1
issue: '7'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '07'
oa: 1
oa_version: Published Version
page: 1117-1119
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Molecular Plant
publication_identifier:
eissn:
- 1674-2052
issn:
- 1752-9867
publication_status: published
publisher: 'Elsevier '
quality_controlled: '1'
scopus_import: '1'
status: public
title: Distinct functions of TIR1 and AFB1 receptors in auxin signalling.
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 16
year: '2023'
...
---
_id: '13266'
abstract:
- lang: eng
text: The 3′,5′-cyclic adenosine monophosphate (cAMP) is a versatile second messenger
in many mammalian signaling pathways. However, its role in plants remains not
well-recognized. Recent discovery of adenylate cyclase (AC) activity for transport
inhibitor response 1/auxin-signaling F-box proteins (TIR1/AFB) auxin receptors
and the demonstration of its importance for canonical auxin signaling put plant
cAMP research back into spotlight. This insight briefly summarizes the well-established
cAMP signaling pathways in mammalian cells and describes the turbulent and controversial
history of plant cAMP research highlighting the major progress and the unresolved
points. We also briefly review the current paradigm of auxin signaling to provide
a background for the discussion on the AC activity of TIR1/AFB auxin receptors
and its potential role in transcriptional auxin signaling as well as impact of
these discoveries on plant cAMP research in general.
acknowledgement: 'We gratefully acknowledge our brave colleagues, whose excellent
efforts kept the plant cAMP research going in the last two decades. The authors
were financially supported by the Austrian Science Fund (FWF): I 6123 and P 37051-B.'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Linlin
full_name: Qi, Linlin
id: 44B04502-A9ED-11E9-B6FC-583AE6697425
last_name: Qi
orcid: 0000-0001-5187-8401
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Qi L, Friml J. Tale of cAMP as a second messenger in auxin signaling and beyond.
New Phytologist. 2023;240(2):489-495. doi:10.1111/nph.19123
apa: Qi, L., & Friml, J. (2023). Tale of cAMP as a second messenger in auxin
signaling and beyond. New Phytologist. Wiley. https://doi.org/10.1111/nph.19123
chicago: Qi, Linlin, and Jiří Friml. “Tale of CAMP as a Second Messenger in Auxin
Signaling and Beyond.” New Phytologist. Wiley, 2023. https://doi.org/10.1111/nph.19123.
ieee: L. Qi and J. Friml, “Tale of cAMP as a second messenger in auxin signaling
and beyond,” New Phytologist, vol. 240, no. 2. Wiley, pp. 489–495, 2023.
ista: Qi L, Friml J. 2023. Tale of cAMP as a second messenger in auxin signaling
and beyond. New Phytologist. 240(2), 489–495.
mla: Qi, Linlin, and Jiří Friml. “Tale of CAMP as a Second Messenger in Auxin Signaling
and Beyond.” New Phytologist, vol. 240, no. 2, Wiley, 2023, pp. 489–95,
doi:10.1111/nph.19123.
short: L. Qi, J. Friml, New Phytologist 240 (2023) 489–495.
date_created: 2023-07-23T22:01:13Z
date_published: 2023-10-01T00:00:00Z
date_updated: 2024-01-29T11:21:55Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/nph.19123
external_id:
isi:
- '001026321500001'
pmid:
- '37434303'
file:
- access_level: open_access
checksum: 6d9bbd45b8e7bb3ceee2586d447bacb2
content_type: application/pdf
creator: dernst
date_created: 2024-01-29T11:21:43Z
date_updated: 2024-01-29T11:21:43Z
file_id: '14898'
file_name: 2023_NewPhytologist_Qi.pdf
file_size: 974464
relation: main_file
success: 1
file_date_updated: 2024-01-29T11:21:43Z
has_accepted_license: '1'
intvolume: ' 240'
isi: 1
issue: '2'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
page: 489-495
pmid: 1
project:
- _id: bd76d395-d553-11ed-ba76-f678c14f9033
grant_number: I06123
name: Peptide receptor complexes for auxin canalization and regeneration in Arabidopsis
- _id: 7bcece63-9f16-11ee-852c-ae94e099eeb6
grant_number: P37051
name: Guanylate cyclase activity of TIR1/AFBs auxin receptors
publication: New Phytologist
publication_identifier:
eissn:
- 1469-8137
issn:
- 0028-646X
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Tale of cAMP as a second messenger in auxin signaling and beyond
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 240
year: '2023'
...
---
_id: '13209'
abstract:
- lang: eng
text: The phytohormone auxin plays central roles in many growth and developmental
processes in plants. Development of chemical tools targeting the auxin pathway
is useful for both plant biology and agriculture. Here we reveal that naproxen,
a synthetic compound with anti-inflammatory activity in humans, acts as an auxin
transport inhibitor targeting PIN-FORMED (PIN) transporters in plants. Physiological
experiments indicate that exogenous naproxen treatment affects pleiotropic auxin-regulated
developmental processes. Additional cellular and biochemical evidence indicates
that naproxen suppresses auxin transport, specifically PIN-mediated auxin efflux.
Moreover, biochemical and structural analyses confirm that naproxen binds directly
to PIN1 protein via the same binding cavity as the indole-3-acetic acid substrate.
Thus, by combining cellular, biochemical, and structural approaches, this study
clearly establishes that naproxen is a PIN inhibitor and elucidates the underlying
mechanisms. Further use of this compound may advance our understanding of the
molecular mechanisms of PIN-mediated auxin transport and expand our toolkit in
auxin biology and agriculture.
acknowledgement: "This work was supported by the Strategic Priority Research Program
of the Chinese Academy of Sciences (XDB37020103 to Linfeng Sun); research funds
from the Center for Advanced Interdisciplinary Science\r\nand Biomedicine of IHM,
Division of Life Sciences and Medicine, University of Science and Technology of
China (QYPY20220012 to S.T.); start-up funding from the University of Science and
Technology of China and the\r\nChinese Academy of Sciences (GG9100007007, KY9100000026,KY9100000051,
and KJ2070000079 to S.T.); the National Natural Science Foundation of China (31900885
to X.L. and 31870732 to Linfeng Sun); the Natural Science Foundation of Anhui Province
(2008085MC90 to X.L. and 2008085J15 to Linfeng Sun); the Fundamental Research Funds
for the Central Universities (WK9100000021 to S.T. and WK9100000031 to Linfeng Sun);
and the USTC Research Funds of the Double First-Class Initiative (YD9100002016 to
S.T. and YD9100002004 to Linfeng Sun). Linfeng Sun is supported by an Outstanding
Young Scholar Award from the Qiu Shi Science and Technologies Foundation and a Young
Scholar Award from the Cyrus Tang Foundation.We thank Dr. Yang Zhao for sharing
published materials (Center for Excellence in Molecular Plant Sciences, Chinese
Academy of Sciences) and the Cryo-EM Center of the University of Science and Technology
of China for the EM facility support. We are grateful to Y. Gao and all other staff
members for their technical support on cryo-EM data collection. "
article_number: '100632'
article_processing_charge: Yes
article_type: original
author:
- first_name: Jing
full_name: Xia, Jing
last_name: Xia
- first_name: Mengjuan
full_name: Kong, Mengjuan
last_name: Kong
- first_name: Zhisen
full_name: Yang, Zhisen
last_name: Yang
- first_name: Lianghanxiao
full_name: Sun, Lianghanxiao
last_name: Sun
- first_name: Yakun
full_name: Peng, Yakun
last_name: Peng
- first_name: Yanbo
full_name: Mao, Yanbo
last_name: Mao
- first_name: Hong
full_name: Wei, Hong
last_name: Wei
- first_name: Wei
full_name: Ying, Wei
last_name: Ying
- first_name: Yongxiao
full_name: Gao, Yongxiao
last_name: Gao
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Jianping
full_name: Weng, Jianping
last_name: Weng
- first_name: Xin
full_name: Liu, Xin
last_name: Liu
- first_name: Linfeng
full_name: Sun, Linfeng
last_name: Sun
- first_name: Shutang
full_name: Tan, Shutang
last_name: Tan
citation:
ama: Xia J, Kong M, Yang Z, et al. Chemical inhibition of Arabidopsis PIN-FORMED
auxin transporters by the anti-inflammatory drug naproxen. Plant Communications.
2023;4(6). doi:10.1016/j.xplc.2023.100632
apa: Xia, J., Kong, M., Yang, Z., Sun, L., Peng, Y., Mao, Y., … Tan, S. (2023).
Chemical inhibition of Arabidopsis PIN-FORMED auxin transporters by the anti-inflammatory
drug naproxen. Plant Communications. Elsevier . https://doi.org/10.1016/j.xplc.2023.100632
chicago: Xia, Jing, Mengjuan Kong, Zhisen Yang, Lianghanxiao Sun, Yakun Peng, Yanbo
Mao, Hong Wei, et al. “Chemical Inhibition of Arabidopsis PIN-FORMED Auxin Transporters
by the Anti-Inflammatory Drug Naproxen.” Plant Communications. Elsevier
, 2023. https://doi.org/10.1016/j.xplc.2023.100632.
ieee: J. Xia et al., “Chemical inhibition of Arabidopsis PIN-FORMED auxin
transporters by the anti-inflammatory drug naproxen,” Plant Communications,
vol. 4, no. 6. Elsevier , 2023.
ista: Xia J, Kong M, Yang Z, Sun L, Peng Y, Mao Y, Wei H, Ying W, Gao Y, Friml J,
Weng J, Liu X, Sun L, Tan S. 2023. Chemical inhibition of Arabidopsis PIN-FORMED
auxin transporters by the anti-inflammatory drug naproxen. Plant Communications.
4(6), 100632.
mla: Xia, Jing, et al. “Chemical Inhibition of Arabidopsis PIN-FORMED Auxin Transporters
by the Anti-Inflammatory Drug Naproxen.” Plant Communications, vol. 4,
no. 6, 100632, Elsevier , 2023, doi:10.1016/j.xplc.2023.100632.
short: J. Xia, M. Kong, Z. Yang, L. Sun, Y. Peng, Y. Mao, H. Wei, W. Ying, Y. Gao,
J. Friml, J. Weng, X. Liu, L. Sun, S. Tan, Plant Communications 4 (2023).
date_created: 2023-07-12T07:32:00Z
date_published: 2023-11-13T00:00:00Z
date_updated: 2024-01-30T10:55:34Z
day: '13'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.xplc.2023.100632
external_id:
isi:
- '001113003000001'
pmid:
- '37254481'
file:
- access_level: open_access
checksum: f8ef92af6096834f91ce38587fb1db9f
content_type: application/pdf
creator: dernst
date_created: 2024-01-30T10:54:40Z
date_updated: 2024-01-30T10:54:40Z
file_id: '14900'
file_name: 2023_PlantCommunications_Xia.pdf
file_size: 1434862
relation: main_file
success: 1
file_date_updated: 2024-01-30T10:54:40Z
has_accepted_license: '1'
intvolume: ' 4'
isi: 1
issue: '6'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
publication: Plant Communications
publication_identifier:
eissn:
- 2590-3462
publication_status: published
publisher: 'Elsevier '
quality_controlled: '1'
status: public
title: Chemical inhibition of Arabidopsis PIN-FORMED auxin transporters by the anti-inflammatory
drug naproxen
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 4
year: '2023'
...
---
_id: '13201'
abstract:
- lang: eng
text: As a crucial nitrogen source, nitrate (NO3−) is a key nutrient for plants.
Accordingly, root systems adapt to maximize NO3− availability, a developmental
regulation also involving the phytohormone auxin. Nonetheless, the molecular mechanisms
underlying this regulation remain poorly understood. Here, we identify low-nitrate-resistant
mutant (lonr) in Arabidopsis (Arabidopsis thaliana), whose root growth fails to
adapt to low-NO3− conditions. lonr2 is defective in the high-affinity NO3− transporter
NRT2.1. lonr2 (nrt2.1) mutants exhibit defects in polar auxin transport, and their
low-NO3−-induced root phenotype depends on the PIN7 auxin exporter activity. NRT2.1
directly associates with PIN7 and antagonizes PIN7-mediated auxin efflux depending
on NO3− levels. These results reveal a mechanism by which NRT2.1 in response to
NO3− limitation directly regulates auxin transport activity and, thus, root growth.
This adaptive mechanism contributes to the root developmental plasticity to help
plants cope with changes in NO3− availability.
acknowledgement: We are grateful to Caifu Jiang for providing ethyl metha-nesulfonate-
mutagenized population, Yi Wang for providing Xenopus oocytes, Jun Fan and Zhaosheng
Kong for providing tobacco BY- 2 cells, and Claus Schwechheimer, Alain Gojon, and
Shutang Tan for helpful discussions. This work was supported by the National Key
Research and Development Program of China (2021YFF1000500), the National Natural Science Foundation of China (32170265 and 32022007), Hainan Provincial Natural Science Foundation of China (323CXTD379), Chinese Universities Scientific Fund (2023TC019), Beijing Municipal Natural Science Foundation (5192011), Beijing Outstanding University Discipline Program, and China
Postdoctoral Science Foundation (BH2020259460).
article_number: e2221313120
article_processing_charge: No
article_type: original
author:
- first_name: Yalu
full_name: Wang, Yalu
last_name: Wang
- first_name: Zhi
full_name: Yuan, Zhi
last_name: Yuan
- first_name: Jinyi
full_name: Wang, Jinyi
last_name: Wang
- first_name: Huixin
full_name: Xiao, Huixin
last_name: Xiao
- first_name: Lu
full_name: Wan, Lu
last_name: Wan
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Yan
full_name: Guo, Yan
last_name: Guo
- first_name: Zhizhong
full_name: Gong, Zhizhong
last_name: Gong
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Jing
full_name: Zhang, Jing
last_name: Zhang
citation:
ama: Wang Y, Yuan Z, Wang J, et al. The nitrate transporter NRT2.1 directly antagonizes
PIN7-mediated auxin transport for root growth adaptation. Proceedings of the
National Academy of Sciences of the United States of America. 2023;120(25).
doi:10.1073/pnas.2221313120
apa: Wang, Y., Yuan, Z., Wang, J., Xiao, H., Wan, L., Li, L., … Zhang, J. (2023).
The nitrate transporter NRT2.1 directly antagonizes PIN7-mediated auxin transport
for root growth adaptation. Proceedings of the National Academy of Sciences
of the United States of America. National Academy of Sciences. https://doi.org/10.1073/pnas.2221313120
chicago: Wang, Yalu, Zhi Yuan, Jinyi Wang, Huixin Xiao, Lu Wan, Lanxin Li, Yan Guo,
Zhizhong Gong, Jiří Friml, and Jing Zhang. “The Nitrate Transporter NRT2.1 Directly
Antagonizes PIN7-Mediated Auxin Transport for Root Growth Adaptation.” Proceedings
of the National Academy of Sciences of the United States of America. National
Academy of Sciences, 2023. https://doi.org/10.1073/pnas.2221313120.
ieee: Y. Wang et al., “The nitrate transporter NRT2.1 directly antagonizes
PIN7-mediated auxin transport for root growth adaptation,” Proceedings of the
National Academy of Sciences of the United States of America, vol. 120, no.
25. National Academy of Sciences, 2023.
ista: Wang Y, Yuan Z, Wang J, Xiao H, Wan L, Li L, Guo Y, Gong Z, Friml J, Zhang
J. 2023. The nitrate transporter NRT2.1 directly antagonizes PIN7-mediated auxin
transport for root growth adaptation. Proceedings of the National Academy of Sciences
of the United States of America. 120(25), e2221313120.
mla: Wang, Yalu, et al. “The Nitrate Transporter NRT2.1 Directly Antagonizes PIN7-Mediated
Auxin Transport for Root Growth Adaptation.” Proceedings of the National Academy
of Sciences of the United States of America, vol. 120, no. 25, e2221313120,
National Academy of Sciences, 2023, doi:10.1073/pnas.2221313120.
short: Y. Wang, Z. Yuan, J. Wang, H. Xiao, L. Wan, L. Li, Y. Guo, Z. Gong, J. Friml,
J. Zhang, Proceedings of the National Academy of Sciences of the United States
of America 120 (2023).
date_created: 2023-07-09T22:01:12Z
date_published: 2023-06-12T00:00:00Z
date_updated: 2023-12-13T23:30:04Z
day: '12'
ddc:
- '570'
department:
- _id: JiFr
doi: 10.1073/pnas.2221313120
external_id:
isi:
- '001030689600003'
pmid:
- '37307446'
file:
- access_level: open_access
checksum: d800e06252eaefba28531fa9440f23f0
content_type: application/pdf
creator: alisjak
date_created: 2023-07-10T08:48:40Z
date_updated: 2023-12-13T23:30:03Z
embargo: 2023-12-12
file_id: '13204'
file_name: 2023_PNAS_Wang.pdf
file_size: 5244581
relation: main_file
file_date_updated: 2023-12-13T23:30:03Z
has_accepted_license: '1'
intvolume: ' 120'
isi: 1
issue: '25'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
pmid: 1
publication: Proceedings of the National Academy of Sciences of the United States
of America
publication_identifier:
eissn:
- 1091-6490
issn:
- 0027-8424
publication_status: published
publisher: National Academy of Sciences
quality_controlled: '1'
scopus_import: '1'
status: public
title: The nitrate transporter NRT2.1 directly antagonizes PIN7-mediated auxin transport
for root growth adaptation
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 120
year: '2023'
...
---
_id: '14510'
acknowledged_ssus:
- _id: EM-Fac
- _id: Bio
- _id: LifeSc
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Nataliia
full_name: Gnyliukh, Nataliia
id: 390C1120-F248-11E8-B48F-1D18A9856A87
last_name: Gnyliukh
orcid: 0000-0002-2198-0509
citation:
ama: Gnyliukh N. Mechanism of clathrin-coated vesicle formation during endocytosis
in plants. 2023. doi:10.15479/at:ista:14510
apa: Gnyliukh, N. (2023). Mechanism of clathrin-coated vesicle formation during
endocytosis in plants. Institute of Science and Technology Austria. https://doi.org/10.15479/at:ista:14510
chicago: Gnyliukh, Nataliia. “Mechanism of Clathrin-Coated Vesicle Formation during
Endocytosis in Plants.” Institute of Science and Technology Austria, 2023. https://doi.org/10.15479/at:ista:14510.
ieee: N. Gnyliukh, “Mechanism of clathrin-coated vesicle formation during endocytosis
in plants,” Institute of Science and Technology Austria, 2023.
ista: Gnyliukh N. 2023. Mechanism of clathrin-coated vesicle formation during endocytosis
in plants. Institute of Science and Technology Austria.
mla: Gnyliukh, Nataliia. Mechanism of Clathrin-Coated Vesicle Formation during
Endocytosis in Plants. Institute of Science and Technology Austria, 2023,
doi:10.15479/at:ista:14510.
short: N. Gnyliukh, Mechanism of Clathrin-Coated Vesicle Formation during Endocytosis
in Plants, Institute of Science and Technology Austria, 2023.
date_created: 2023-11-10T09:10:06Z
date_published: 2023-11-10T00:00:00Z
date_updated: 2024-03-27T23:30:45Z
day: '10'
ddc:
- '570'
degree_awarded: PhD
department:
- _id: GradSch
- _id: JiFr
- _id: MaLo
doi: 10.15479/at:ista:14510
ec_funded: 1
file:
- access_level: closed
checksum: 3d5e680bfc61f98e308c434f45cc9bd6
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: ngnyliuk
date_created: 2023-11-20T09:18:51Z
date_updated: 2023-11-20T09:18:51Z
file_id: '14567'
file_name: Thesis_Gnyliukh_final_08_11_23.docx
file_size: 20824903
relation: source_file
- access_level: closed
checksum: bfc96d47fc4e7e857dd71656097214a4
content_type: application/pdf
creator: ngnyliuk
date_created: 2023-11-20T09:23:11Z
date_updated: 2023-11-23T13:10:55Z
embargo: 2024-11-23
embargo_to: open_access
file_id: '14568'
file_name: Thesis_Gnyliukh_final_20_11_23.pdf
file_size: 24871844
relation: main_file
file_date_updated: 2023-11-23T13:10:55Z
has_accepted_license: '1'
keyword:
- Clathrin-Mediated Endocytosis
- vesicle scission
- Dynamin-Related Protein 2
- SH3P2
- TPLATE complex
- Total internal reflection fluorescence microscopy
- Arabidopsis thaliana
language:
- iso: eng
month: '11'
oa_version: Published Version
page: '180'
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
publication_identifier:
isbn:
- 978-3-99078-037-4
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '14591'
relation: part_of_dissertation
status: public
- id: '9887'
relation: part_of_dissertation
status: public
- id: '8139'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Martin
full_name: Loose, Martin
id: 462D4284-F248-11E8-B48F-1D18A9856A87
last_name: Loose
orcid: 0000-0001-7309-9724
title: Mechanism of clathrin-coated vesicle formation during endocytosis in plants
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: dissertation
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
year: '2023'
...
---
_id: '10016'
abstract:
- lang: eng
text: 'Auxin has always been at the forefront of research in plant physiology and
development. Since the earliest contemplations by Julius von Sachs and Charles
Darwin, more than a century-long struggle has been waged to understand its function.
This largely reflects the failures, successes, and inevitable progress in the
entire field of plant signaling and development. Here I present 14 stations on
our long and sometimes mystical journey to understand auxin. These highlights
were selected to give a flavor of the field and to show the scope and limits of
our current knowledge. A special focus is put on features that make auxin unique
among phytohormones, such as its dynamic, directional transport network, which
integrates external and internal signals, including self-organizing feedback.
Accented are persistent mysteries and controversies. The unexpected discoveries
related to rapid auxin responses and growth regulation recently disturbed our
contentment regarding understanding of the auxin signaling mechanism. These new
revelations, along with advances in technology, usher us into a new, exciting
era in auxin research. '
acknowledgement: "The author thanks the whole community of researchers consciously
or unconsciously working on questions related to auxin, whose hard work and enthusiasm
contributed to development of this exciting story. Particular thanks go to many\r\nbrilliant
present and past members of the Friml group and our numerous excellent collaborators,
without whom my own personal journey would not be possible. The way of the cross
with its 14 stations is a popular devotion among Roman Catholics and inspires them
to make a spiritual pilgrimage through contemplation of Christ on his last day.
Its aspects of gradual progress, struggle, passion, and revelation served as an
inspiration for the formal depiction of our journey to understanding auxin as described
in this review. It is in no way intended to reflect the personal beliefs of the
author and readers. I am grateful to Nick Barton, Eva Benková, Lenka Caisová, Matyáš
Fendrych, Lukáš Fiedler, Monika Frátriková, Jarmila Frimlová, Michelle Gallei, Jakub
Hajný, Lukas Hoermayer, Alexandra Mally, Ondrˇej Novák, Jan Petrášek, Aleš Pěnčík,
Steffen Vanneste, Tongda Xu, and Zhenbiao Yang for their valuable comments. Special
thanks go to Michelle Gallei for her invaluable assistance with the figures."
article_number: a039859
article_processing_charge: No
article_type: review
author:
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Friml J. Fourteen stations of auxin. Cold Spring Harbor Perspectives in
Biology. 2022;14(5). doi:10.1101/cshperspect.a039859
apa: Friml, J. (2022). Fourteen stations of auxin. Cold Spring Harbor Perspectives
in Biology. Cold Spring Harbor Laboratory. https://doi.org/10.1101/cshperspect.a039859
chicago: Friml, Jiří. “Fourteen Stations of Auxin.” Cold Spring Harbor Perspectives
in Biology. Cold Spring Harbor Laboratory, 2022. https://doi.org/10.1101/cshperspect.a039859 .
ieee: J. Friml, “Fourteen stations of auxin,” Cold Spring Harbor Perspectives
in Biology, vol. 14, no. 5. Cold Spring Harbor Laboratory, 2022.
ista: Friml J. 2022. Fourteen stations of auxin. Cold Spring Harbor Perspectives
in Biology. 14(5), a039859.
mla: Friml, Jiří. “Fourteen Stations of Auxin.” Cold Spring Harbor Perspectives
in Biology, vol. 14, no. 5, a039859, Cold Spring Harbor Laboratory, 2022,
doi:10.1101/cshperspect.a039859
.
short: J. Friml, Cold Spring Harbor Perspectives in Biology 14 (2022).
date_created: 2021-09-14T11:36:53Z
date_published: 2022-05-27T00:00:00Z
date_updated: 2023-08-02T06:54:42Z
day: '27'
department:
- _id: JiFr
doi: '10.1101/cshperspect.a039859 '
external_id:
isi:
- '000806563000003'
pmid:
- '34400554'
intvolume: ' 14'
isi: 1
issue: '5'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: 'https://doi.org/10.1101/cshperspect.a039859 '
month: '05'
oa: 1
oa_version: Published Version
pmid: 1
publication: Cold Spring Harbor Perspectives in Biology
publication_identifier:
issn:
- 1943-0264
publication_status: published
publisher: Cold Spring Harbor Laboratory
quality_controlled: '1'
scopus_import: '1'
status: public
title: Fourteen stations of auxin
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 14
year: '2022'
...
---
_id: '10583'
abstract:
- lang: eng
text: The synthetic strigolactone (SL) analog, rac-GR24, has been instrumental in
studying the role of SLs as well as karrikins because it activates the receptors
DWARF14 (D14) and KARRIKIN INSENSITIVE 2 (KAI2) of their signaling pathways, respectively.
Treatment with rac-GR24 modifies the root architecture at different levels, such
as decreasing the lateral root density (LRD), while promoting root hair elongation
or flavonol accumulation. Previously, we have shown that the flavonol biosynthesis
is transcriptionally activated in the root by rac-GR24 treatment, but, thus far,
the molecular players involved in that response have remained unknown. To get
an in-depth insight into the changes that occur after the compound is perceived
by the roots, we compared the root transcriptomes of the wild type and the more
axillary growth2 (max2) mutant, affected in both SL and karrikin signaling pathways,
with and without rac-GR24 treatment. Quantitative reverse transcription (qRT)-PCR,
reporter line analysis and mutant phenotyping indicated that the flavonol response
and the root hair elongation are controlled by the ELONGATED HYPOCOTYL 5 (HY5)
and MYB12 transcription factors, but HY5, in contrast to MYB12, affects the LRD
as well. Furthermore, we identified the transcription factors TARGET OF MONOPTEROS
5 (TMO5) and TMO5 LIKE1 as negative and the Mediator complex as positive regulators
of the rac-GR24 effect on LRD. Altogether, hereby, we get closer toward understanding
the molecular mechanisms that underlay the rac-GR24 responses in the root.
acknowledgement: The authors thank Ralf Stracke (Bielefeld University, Bielefeld,
Germany) for providing the myb mutants and their colleagues Bert De Rybel for the
tmo5t;mo5l1 double mutant, Boris Parizot for tips on the RNA-seq analysis, Veronique
Storme for statistical help on both the RNA-seq and lateral root density, and Martine
De Cock for help in preparing the manuscript.
article_processing_charge: No
article_type: original
author:
- first_name: Sylwia
full_name: Struk, Sylwia
last_name: Struk
- first_name: Lukas
full_name: Braem, Lukas
last_name: Braem
- first_name: Cedrick
full_name: Matthys, Cedrick
last_name: Matthys
- first_name: Alan
full_name: Walton, Alan
last_name: Walton
- first_name: Nick
full_name: Vangheluwe, Nick
last_name: Vangheluwe
- first_name: Stan
full_name: Van Praet, Stan
last_name: Van Praet
- first_name: Lingxiang
full_name: Jiang, Lingxiang
last_name: Jiang
- first_name: Pawel
full_name: Baster, Pawel
id: 3028BD74-F248-11E8-B48F-1D18A9856A87
last_name: Baster
- first_name: Carolien
full_name: De Cuyper, Carolien
last_name: De Cuyper
- first_name: Francois-Didier
full_name: Boyer, Francois-Didier
last_name: Boyer
- first_name: Elisabeth
full_name: Stes, Elisabeth
last_name: Stes
- first_name: Tom
full_name: Beeckman, Tom
last_name: Beeckman
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Kris
full_name: Gevaert, Kris
last_name: Gevaert
- first_name: Sofie
full_name: Goormachtig, Sofie
last_name: Goormachtig
citation:
ama: Struk S, Braem L, Matthys C, et al. Transcriptional analysis in the Arabidopsis
roots reveals new regulators that link rac-GR24 treatment with changes in flavonol
accumulation, root hair elongation and lateral root density. Plant & Cell
Physiology. 2022;63(1):104-119. doi:10.1093/pcp/pcab149
apa: Struk, S., Braem, L., Matthys, C., Walton, A., Vangheluwe, N., Van Praet, S.,
… Goormachtig, S. (2022). Transcriptional analysis in the Arabidopsis roots reveals
new regulators that link rac-GR24 treatment with changes in flavonol accumulation,
root hair elongation and lateral root density. Plant & Cell Physiology.
Oxford University Press. https://doi.org/10.1093/pcp/pcab149
chicago: Struk, Sylwia, Lukas Braem, Cedrick Matthys, Alan Walton, Nick Vangheluwe,
Stan Van Praet, Lingxiang Jiang, et al. “Transcriptional Analysis in the Arabidopsis
Roots Reveals New Regulators That Link Rac-GR24 Treatment with Changes in Flavonol
Accumulation, Root Hair Elongation and Lateral Root Density.” Plant & Cell
Physiology. Oxford University Press, 2022. https://doi.org/10.1093/pcp/pcab149.
ieee: S. Struk et al., “Transcriptional analysis in the Arabidopsis roots
reveals new regulators that link rac-GR24 treatment with changes in flavonol accumulation,
root hair elongation and lateral root density,” Plant & Cell Physiology,
vol. 63, no. 1. Oxford University Press, pp. 104–119, 2022.
ista: Struk S, Braem L, Matthys C, Walton A, Vangheluwe N, Van Praet S, Jiang L,
Baster P, De Cuyper C, Boyer F-D, Stes E, Beeckman T, Friml J, Gevaert K, Goormachtig
S. 2022. Transcriptional analysis in the Arabidopsis roots reveals new regulators
that link rac-GR24 treatment with changes in flavonol accumulation, root hair
elongation and lateral root density. Plant & Cell Physiology. 63(1), 104–119.
mla: Struk, Sylwia, et al. “Transcriptional Analysis in the Arabidopsis Roots Reveals
New Regulators That Link Rac-GR24 Treatment with Changes in Flavonol Accumulation,
Root Hair Elongation and Lateral Root Density.” Plant & Cell Physiology,
vol. 63, no. 1, Oxford University Press, 2022, pp. 104–19, doi:10.1093/pcp/pcab149.
short: S. Struk, L. Braem, C. Matthys, A. Walton, N. Vangheluwe, S. Van Praet, L.
Jiang, P. Baster, C. De Cuyper, F.-D. Boyer, E. Stes, T. Beeckman, J. Friml, K.
Gevaert, S. Goormachtig, Plant & Cell Physiology 63 (2022) 104–119.
date_created: 2021-12-28T11:44:18Z
date_published: 2022-01-21T00:00:00Z
date_updated: 2023-08-02T13:40:43Z
day: '21'
department:
- _id: JiFr
doi: 10.1093/pcp/pcab149
external_id:
isi:
- '000877899400009'
pmid:
- '34791413'
intvolume: ' 63'
isi: 1
issue: '1'
keyword:
- flavonols
- MAX2
- rac-Gr24
- RNA-seq
- root development
- transcriptional regulation
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1093/pcp/pcab149
month: '01'
oa: 1
oa_version: Published Version
page: 104-119
pmid: 1
publication: Plant & Cell Physiology
publication_identifier:
eissn:
- 1471-9053
issn:
- 0032-0781
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Transcriptional analysis in the Arabidopsis roots reveals new regulators that
link rac-GR24 treatment with changes in flavonol accumulation, root hair elongation
and lateral root density
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 63
year: '2022'
...
---
_id: '10717'
abstract:
- lang: eng
text: Much of what we know about the role of auxin in plant development derives
from exogenous manipulations of auxin distribution and signaling, using inhibitors,
auxins and auxin analogs. In this context, synthetic auxin analogs, such as 1-Naphtalene
Acetic Acid (1-NAA), are often favored over the endogenous auxin indole-3-acetic
acid (IAA), in part due to their higher stability. While such auxin analogs have
proven to be instrumental to reveal the various faces of auxin, they display in
some cases distinct bioactivities compared to IAA. Here, we focused on the effect
of auxin analogs on the accumulation of PIN proteins in Brefeldin A-sensitive
endosomal aggregations (BFA bodies), and the correlation with the ability to elicit
Ca 2+ responses. For a set of commonly used auxin analogs, we evaluated if auxin-analog
induced Ca 2+ signaling inhibits PIN accumulation. Not all auxin analogs elicited
a Ca 2+ response, and their differential ability to elicit Ca 2+ responses correlated
partially with their ability to inhibit BFA-body formation. However, in tir1/afb
and cngc14, 1-NAA-induced Ca 2+ signaling was strongly impaired, yet 1-NAA still
could inhibit PIN accumulation in BFA bodies. This demonstrates that TIR1/AFB-CNGC14-dependent
Ca 2+ signaling does not inhibit BFA body formation in Arabidopsis roots.
acknowledgement: "We thank Joerg Kudla (WWU Munster, Germany), Petra Dietrich (F.A.
University of Erlangen-Nurnberg, Germany) for sharing published materials, and NASC
for providing seeds. We thank Veronique Storme for help with the statistical analyses.
Part of the imaging analysis was carried out at NOLIMITS, an advanced imaging facility
established by the University of Milan.\r\nThis work was supported by grants of
the China Scholarship Council (CSC) to RW and JC; Fonds Wetenschappelijk Onderzoek
(FWO) to TB and (G002220N) SV; the special research fund of Ghent University to
EH; the Deutsche Forschungsgemeinschaft (DFG) through Grants within FOR964 (MK and
KS); Piano di Sviluppo di Ateneo 2019 (University of Milan) to AC; the European
Research Council (ERC) T-Rex project 682436 to DVD; the ERC ETAP project 742985
to JF, and by a PhD fellowship from the University of Milan to MG."
article_number: erac019
article_processing_charge: No
article_type: original
author:
- first_name: R
full_name: Wang, R
last_name: Wang
- first_name: E
full_name: Himschoot, E
last_name: Himschoot
- first_name: M
full_name: Grenzi, M
last_name: Grenzi
- first_name: J
full_name: Chen, J
last_name: Chen
- first_name: A
full_name: Safi, A
last_name: Safi
- first_name: M
full_name: Krebs, M
last_name: Krebs
- first_name: K
full_name: Schumacher, K
last_name: Schumacher
- first_name: MK
full_name: Nowack, MK
last_name: Nowack
- first_name: W
full_name: Moeder, W
last_name: Moeder
- first_name: K
full_name: Yoshioka, K
last_name: Yoshioka
- first_name: D
full_name: Van Damme, D
last_name: Van Damme
- first_name: I
full_name: De Smet, I
last_name: De Smet
- first_name: D
full_name: Geelen, D
last_name: Geelen
- first_name: T
full_name: Beeckman, T
last_name: Beeckman
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: A
full_name: Costa, A
last_name: Costa
- first_name: S
full_name: Vanneste, S
last_name: Vanneste
citation:
ama: Wang R, Himschoot E, Grenzi M, et al. Auxin analog-induced Ca2+ signaling is
independent of inhibition of endosomal aggregation in Arabidopsis roots. Journal
of Experimental Botany. 2022;73(8). doi:10.1093/jxb/erac019
apa: Wang, R., Himschoot, E., Grenzi, M., Chen, J., Safi, A., Krebs, M., … Vanneste,
S. (2022). Auxin analog-induced Ca2+ signaling is independent of inhibition of
endosomal aggregation in Arabidopsis roots. Journal of Experimental Botany.
Oxford Academic. https://doi.org/10.1093/jxb/erac019
chicago: Wang, R, E Himschoot, M Grenzi, J Chen, A Safi, M Krebs, K Schumacher,
et al. “Auxin Analog-Induced Ca2+ Signaling Is Independent of Inhibition of Endosomal
Aggregation in Arabidopsis Roots.” Journal of Experimental Botany. Oxford
Academic, 2022. https://doi.org/10.1093/jxb/erac019.
ieee: R. Wang et al., “Auxin analog-induced Ca2+ signaling is independent
of inhibition of endosomal aggregation in Arabidopsis roots,” Journal of Experimental
Botany, vol. 73, no. 8. Oxford Academic, 2022.
ista: Wang R, Himschoot E, Grenzi M, Chen J, Safi A, Krebs M, Schumacher K, Nowack
M, Moeder W, Yoshioka K, Van Damme D, De Smet I, Geelen D, Beeckman T, Friml J,
Costa A, Vanneste S. 2022. Auxin analog-induced Ca2+ signaling is independent
of inhibition of endosomal aggregation in Arabidopsis roots. Journal of Experimental
Botany. 73(8), erac019.
mla: Wang, R., et al. “Auxin Analog-Induced Ca2+ Signaling Is Independent of Inhibition
of Endosomal Aggregation in Arabidopsis Roots.” Journal of Experimental Botany,
vol. 73, no. 8, erac019, Oxford Academic, 2022, doi:10.1093/jxb/erac019.
short: R. Wang, E. Himschoot, M. Grenzi, J. Chen, A. Safi, M. Krebs, K. Schumacher,
M. Nowack, W. Moeder, K. Yoshioka, D. Van Damme, I. De Smet, D. Geelen, T. Beeckman,
J. Friml, A. Costa, S. Vanneste, Journal of Experimental Botany 73 (2022).
date_created: 2022-02-03T09:19:01Z
date_published: 2022-04-18T00:00:00Z
date_updated: 2023-08-02T14:07:58Z
day: '18'
department:
- _id: JiFr
doi: 10.1093/jxb/erac019
ec_funded: 1
external_id:
isi:
- '000764220900001'
pmid:
- '35085386'
intvolume: ' 73'
isi: 1
issue: '8'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://biblio.ugent.be/publication/8738721
month: '04'
oa: 1
oa_version: Submitted Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Journal of Experimental Botany
publication_identifier:
eissn:
- 1460-2431
issn:
- 0022-0957
publication_status: published
publisher: Oxford Academic
quality_controlled: '1'
scopus_import: '1'
status: public
title: Auxin analog-induced Ca2+ signaling is independent of inhibition of endosomal
aggregation in Arabidopsis roots
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 73
year: '2022'
...
---
_id: '10719'
abstract:
- lang: eng
text: Auxin, one of the first identified and most widely studied phytohormones,
has been and will remain a hot topic in plant biology. After more than a century
of passionate exploration, the mysteries of its synthesis, transport, signaling,
and metabolism have largely been unlocked. Due to the rapid development of new
technologies, new methods, and new genetic materials, the study of auxin has entered
the fast lane over the past 30 years. Here, we highlight advances in understanding
auxin signaling, including auxin perception, rapid auxin responses, TRANSPORT
INHIBITOR RESPONSE 1 and AUXIN SIGNALING F-boxes (TIR1/AFBs)-mediated transcriptional
and non-transcriptional branches, and the epigenetic regulation of auxin signaling.
We also focus on feedback inhibition mechanisms that prevent the over-amplification
of auxin signals. In addition, we cover the TRANSMEMBRANE KINASEs (TMKs)-mediated
non-canonical signaling, which converges with TIR1/AFBs-mediated transcriptional
regulation to coordinate plant growth and development. The identification of additional
auxin signaling components and their regulation will continue to open new avenues
of research in this field, leading to an increasingly deeper, more comprehensive
understanding of how auxin signals are interpreted at the cellular level to regulate
plant growth and development.
acknowledgement: "This research was financially supported by the National Natural
Science Foundation of China and the Israel Science Foundation (NSFC-ISF; 32061143005),
National Natural Science Foundation of China (32000225), Natural Science Foundation
of Shandong Province (ZR2020QC036), and China Postdoctoral Science Foundation (2020M682165).\r\n"
article_processing_charge: No
article_type: review
author:
- first_name: Z
full_name: Yu, Z
last_name: Yu
- first_name: F
full_name: Zhang, F
last_name: Zhang
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Z
full_name: Ding, Z
last_name: Ding
citation:
ama: 'Yu Z, Zhang F, Friml J, Ding Z. Auxin signaling: Research advances over the
past 30 years. Journal of Integrative Plant Biology. 2022;64(2):371-392.
doi:10.1111/jipb.13225'
apa: 'Yu, Z., Zhang, F., Friml, J., & Ding, Z. (2022). Auxin signaling: Research
advances over the past 30 years. Journal of Integrative Plant Biology.
Wiley. https://doi.org/10.1111/jipb.13225'
chicago: 'Yu, Z, F Zhang, Jiří Friml, and Z Ding. “Auxin Signaling: Research Advances
over the Past 30 Years.” Journal of Integrative Plant Biology. Wiley, 2022.
https://doi.org/10.1111/jipb.13225.'
ieee: 'Z. Yu, F. Zhang, J. Friml, and Z. Ding, “Auxin signaling: Research advances
over the past 30 years,” Journal of Integrative Plant Biology, vol. 64,
no. 2. Wiley, pp. 371–392, 2022.'
ista: 'Yu Z, Zhang F, Friml J, Ding Z. 2022. Auxin signaling: Research advances
over the past 30 years. Journal of Integrative Plant Biology. 64(2), 371–392.'
mla: 'Yu, Z., et al. “Auxin Signaling: Research Advances over the Past 30 Years.”
Journal of Integrative Plant Biology, vol. 64, no. 2, Wiley, 2022, pp.
371–92, doi:10.1111/jipb.13225.'
short: Z. Yu, F. Zhang, J. Friml, Z. Ding, Journal of Integrative Plant Biology
64 (2022) 371–392.
date_created: 2022-02-03T09:52:59Z
date_published: 2022-02-01T00:00:00Z
date_updated: 2023-08-02T14:08:30Z
day: '01'
department:
- _id: JiFr
doi: 10.1111/jipb.13225
external_id:
isi:
- '000761281200011'
pmid:
- '35018726'
intvolume: ' 64'
isi: 1
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1111/jipb.13225
month: '02'
oa: 1
oa_version: Published Version
page: 371-392
pmid: 1
publication: Journal of Integrative Plant Biology
publication_identifier:
eissn:
- 1744-7909
issn:
- 1672-9072
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Auxin signaling: Research advances over the past 30 years'
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 64
year: '2022'
...
---
_id: '10768'
abstract:
- lang: eng
text: Among the most fascinated properties of the plant hormone auxin is its ability
to promote formation of its own directional transport routes. These gradually
narrowing auxin channels form from the auxin source toward the sink and involve
coordinated, collective polarization of individual cells. Once established, the
channels provide positional information, along which new vascular strands form,
for example, during organogenesis, regeneration, or leave venation. The main prerequisite
of this still mysterious auxin canalization mechanism is a feedback between auxin
signaling and its directional transport. This is manifested by auxin-induced re-arrangements
of polar, subcellular localization of PIN-FORMED (PIN) auxin exporters. Immanent
open questions relate to how position of auxin source and sink as well as tissue
context are sensed and translated into tissue polarization and how cells communicate
to polarize coordinately. Recently, identification of the first molecular players
opens new avenues into molecular studies of this intriguing example of self-organizing
plant development.
acknowledgement: The authors apologize to those researchers whose work was not cited.
In addition, exciting topics such as PIN polarization in context of phyllotaxis,
shoot branching and termination of gravitropic bending, or role of additional auxin
transporters could not have been included owing to lack of space. This work was
supported by the Czech Science Foundation GAČR (GA18-26981S). The authors also acknowledge
the EMBO for supporting J.H. with a long-term fellowship (ALTF217-2021).
article_number: '102174'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Jakub
full_name: Hajny, Jakub
id: 4800CC20-F248-11E8-B48F-1D18A9856A87
last_name: Hajny
orcid: 0000-0003-2140-7195
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Hajny J, Tan S, Friml J. Auxin canalization: From speculative models toward
molecular players. Current Opinion in Plant Biology. 2022;65(2). doi:10.1016/j.pbi.2022.102174'
apa: 'Hajny, J., Tan, S., & Friml, J. (2022). Auxin canalization: From speculative
models toward molecular players. Current Opinion in Plant Biology. Elsevier.
https://doi.org/10.1016/j.pbi.2022.102174'
chicago: 'Hajny, Jakub, Shutang Tan, and Jiří Friml. “Auxin Canalization: From Speculative
Models toward Molecular Players.” Current Opinion in Plant Biology. Elsevier,
2022. https://doi.org/10.1016/j.pbi.2022.102174.'
ieee: 'J. Hajny, S. Tan, and J. Friml, “Auxin canalization: From speculative models
toward molecular players,” Current Opinion in Plant Biology, vol. 65, no.
2. Elsevier, 2022.'
ista: 'Hajny J, Tan S, Friml J. 2022. Auxin canalization: From speculative models
toward molecular players. Current Opinion in Plant Biology. 65(2), 102174.'
mla: 'Hajny, Jakub, et al. “Auxin Canalization: From Speculative Models toward Molecular
Players.” Current Opinion in Plant Biology, vol. 65, no. 2, 102174, Elsevier,
2022, doi:10.1016/j.pbi.2022.102174.'
short: J. Hajny, S. Tan, J. Friml, Current Opinion in Plant Biology 65 (2022).
date_created: 2022-02-20T23:01:32Z
date_published: 2022-02-01T00:00:00Z
date_updated: 2023-08-02T14:29:12Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.pbi.2022.102174
external_id:
isi:
- '000758724700004'
pmid:
- '35123880'
file:
- access_level: open_access
checksum: f1ee02b6fb4200934eeb31fa69120885
content_type: application/pdf
creator: dernst
date_created: 2022-03-10T13:34:09Z
date_updated: 2022-03-10T13:34:09Z
file_id: '10844'
file_name: 2022_CurrentOpPlantBiology_Hajny.pdf
file_size: 820322
relation: main_file
success: 1
file_date_updated: 2022-03-10T13:34:09Z
has_accepted_license: '1'
intvolume: ' 65'
isi: 1
issue: '2'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
publication: Current Opinion in Plant Biology
publication_identifier:
issn:
- 1369-5266
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Auxin canalization: From speculative models toward molecular players'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 65
year: '2022'
...
---
_id: '10841'
abstract:
- lang: eng
text: In eukaryotes, clathrin-coated vesicles (CCVs) facilitate the internalization
of material from the cell surface as well as the movement of cargo in post-Golgi
trafficking pathways. This diversity of functions is partially provided by multiple
monomeric and multimeric clathrin adaptor complexes that provide compartment and
cargo selectivity. The adaptor-protein assembly polypeptide-1 (AP-1) complex operates
as part of the secretory pathway at the trans-Golgi network (TGN), while the AP-2
complex and the TPLATE complex jointly operate at the plasma membrane to execute
clathrin-mediated endocytosis. Key to our further understanding of clathrin-mediated
trafficking in plants will be the comprehensive identification and characterization
of the network of evolutionarily conserved and plant-specific core and accessory
machinery involved in the formation and targeting of CCVs. To facilitate these
studies, we have analyzed the proteome of enriched TGN/early endosome-derived
and endocytic CCVs isolated from dividing and expanding suspension-cultured Arabidopsis
(Arabidopsis thaliana) cells. Tandem mass spectrometry analysis results were validated
by differential chemical labeling experiments to identify proteins co-enriching
with CCVs. Proteins enriched in CCVs included previously characterized CCV components
and cargos such as the vacuolar sorting receptors in addition to conserved and
plant-specific components whose function in clathrin-mediated trafficking has
not been previously defined. Notably, in addition to AP-1 and AP-2, all subunits
of the AP-4 complex, but not AP-3 or AP-5, were found to be in high abundance
in the CCV proteome. The association of AP-4 with suspension-cultured Arabidopsis
CCVs is further supported via additional biochemical data.
acknowledged_ssus:
- _id: EM-Fac
acknowledgement: 'The authors would like to acknowledge the VIB Proteomics Core Facility
(VIB-UGent Center for Medical Biotechnology in Ghent, Belgium) and the Research
Technology Support Facility Proteomics Core (Michigan State University in East Lansing,
Michigan) for sample analysis, as well as the University of Wisconsin Biotechnology
Center Mass Spectrometry Core Facility (Madison, WI) for help with data processing.
Additionally, we are grateful to Sue Weintraub (UT Health San Antonio) and Sydney
Thomas (UW- Madison) for assistance with data analysis. This research was supported
by grants to S.Y.B. from the National Science Foundation (Nos. 1121998 and 1614915)
and a Vilas Associate Award (University of Wisconsin, Madison, Graduate School);
to J.P. from the National Natural Science Foundation of China (Nos. 91754104, 31820103008,
and 31670283); to I.H. from the National Research Foundation of Korea (No. 2019R1A2B5B03099982).
This research was also supported by the Scientific Service Units (SSU) of IST Austria
through resources provided by the Electron microscopy Facility (EMF). A.J. is supported
by funding from the Austrian Science Fund (FWF): I3630B25 to J.F. A.H. is supported
by funding from the National Science Foundation (NSF IOS Nos. 1025837 and 1147032).'
article_processing_charge: No
article_type: original
author:
- first_name: DA
full_name: Dahhan, DA
last_name: Dahhan
- first_name: GD
full_name: Reynolds, GD
last_name: Reynolds
- first_name: JJ
full_name: Cárdenas, JJ
last_name: Cárdenas
- first_name: D
full_name: Eeckhout, D
last_name: Eeckhout
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: K
full_name: Yperman, K
last_name: Yperman
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: N
full_name: Vang, N
last_name: Vang
- first_name: X
full_name: Yan, X
last_name: Yan
- first_name: I
full_name: Hwang, I
last_name: Hwang
- first_name: A
full_name: Heese, A
last_name: Heese
- first_name: G
full_name: De Jaeger, G
last_name: De Jaeger
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: D
full_name: Van Damme, D
last_name: Van Damme
- first_name: J
full_name: Pan, J
last_name: Pan
- first_name: SY
full_name: Bednarek, SY
last_name: Bednarek
citation:
ama: Dahhan D, Reynolds G, Cárdenas J, et al. Proteomic characterization of isolated
Arabidopsis clathrin-coated vesicles reveals evolutionarily conserved and plant-specific
components. Plant Cell. 2022;34(6):2150-2173. doi:10.1093/plcell/koac071
apa: Dahhan, D., Reynolds, G., Cárdenas, J., Eeckhout, D., Johnson, A. J., Yperman,
K., … Bednarek, S. (2022). Proteomic characterization of isolated Arabidopsis
clathrin-coated vesicles reveals evolutionarily conserved and plant-specific components.
Plant Cell. Oxford Academic. https://doi.org/10.1093/plcell/koac071
chicago: Dahhan, DA, GD Reynolds, JJ Cárdenas, D Eeckhout, Alexander J Johnson,
K Yperman, Walter Kaufmann, et al. “Proteomic Characterization of Isolated Arabidopsis
Clathrin-Coated Vesicles Reveals Evolutionarily Conserved and Plant-Specific Components.”
Plant Cell. Oxford Academic, 2022. https://doi.org/10.1093/plcell/koac071.
ieee: D. Dahhan et al., “Proteomic characterization of isolated Arabidopsis
clathrin-coated vesicles reveals evolutionarily conserved and plant-specific components,”
Plant Cell, vol. 34, no. 6. Oxford Academic, pp. 2150–2173, 2022.
ista: Dahhan D, Reynolds G, Cárdenas J, Eeckhout D, Johnson AJ, Yperman K, Kaufmann
W, Vang N, Yan X, Hwang I, Heese A, De Jaeger G, Friml J, Van Damme D, Pan J,
Bednarek S. 2022. Proteomic characterization of isolated Arabidopsis clathrin-coated
vesicles reveals evolutionarily conserved and plant-specific components. Plant
Cell. 34(6), 2150–2173.
mla: Dahhan, DA, et al. “Proteomic Characterization of Isolated Arabidopsis Clathrin-Coated
Vesicles Reveals Evolutionarily Conserved and Plant-Specific Components.” Plant
Cell, vol. 34, no. 6, Oxford Academic, 2022, pp. 2150–73, doi:10.1093/plcell/koac071.
short: D. Dahhan, G. Reynolds, J. Cárdenas, D. Eeckhout, A.J. Johnson, K. Yperman,
W. Kaufmann, N. Vang, X. Yan, I. Hwang, A. Heese, G. De Jaeger, J. Friml, D. Van
Damme, J. Pan, S. Bednarek, Plant Cell 34 (2022) 2150–2173.
date_created: 2022-03-08T13:47:51Z
date_published: 2022-06-01T00:00:00Z
date_updated: 2023-08-02T14:46:48Z
day: '01'
department:
- _id: JiFr
- _id: EM-Fac
doi: 10.1093/plcell/koac071
external_id:
isi:
- '000767438800001'
pmid:
- '35218346'
intvolume: ' 34'
isi: 1
issue: '6'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2021.09.16.460678
month: '06'
oa: 1
oa_version: Preprint
page: 2150-2173
pmid: 1
project:
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: Plant Cell
publication_identifier:
eissn:
- 1532-298x
issn:
- 1040-4651
publication_status: published
publisher: Oxford Academic
quality_controlled: '1'
scopus_import: '1'
status: public
title: Proteomic characterization of isolated Arabidopsis clathrin-coated vesicles
reveals evolutionarily conserved and plant-specific components
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 34
year: '2022'
...
---
_id: '10888'
abstract:
- lang: eng
text: Despite the growing interest in using chemical genetics in plant research,
small molecule target identification remains a major challenge. The cellular thermal
shift assay coupled with high-resolution mass spectrometry (CETSA MS) that monitors
changes in the thermal stability of proteins caused by their interactions with
small molecules, other proteins, or posttranslational modifications, allows the
discovery of drug targets or the study of protein–metabolite and protein–protein
interactions mainly in mammalian cells. To showcase the applicability of this
method in plants, we applied CETSA MS to intact Arabidopsis thaliana cells and
identified the thermal proteome of the plant-specific glycogen synthase kinase
3 (GSK3) inhibitor, bikinin. A comparison between the thermal and the phosphoproteomes
of bikinin revealed the auxin efflux carrier PIN-FORMED1 (PIN1) as a substrate
of the Arabidopsis GSK3s that negatively regulate the brassinosteroid signaling.
We established that PIN1 phosphorylation by the GSK3s is essential for maintaining
its intracellular polarity that is required for auxin-mediated regulation of vascular
patterning in the leaf, thus revealing cross-talk between brassinosteroid and
auxin signaling.
acknowledgement: "We thank Yanhai Yin for providing the anti-BES1 antibody, Johan
Winne and Brenda Callebaut for synthesizing bikinin, Yuki Kondo and Hiroo Fukuda
for published materials, Tomasz Nodzy\x03nski for useful advice, and Martine De
Cock for help in preparing the manuscript. This\r\nwork was supported by the China
Scholarship Council for predoctoral (Q.L. and X.X.) and postdoctoral (Y.Z.) fellowships;
the Agency for Innovation by Science and Technology for a predoctoral fellowship
(W.D.); the Research Foundation-Flanders, Projects G009018N and G002121N (E.R.);
and the VIB TechWatch Fund (E.R.)."
article_number: e2118220119
article_processing_charge: No
article_type: original
author:
- first_name: Qing
full_name: Lu, Qing
last_name: Lu
- first_name: Yonghong
full_name: Zhang, Yonghong
last_name: Zhang
- first_name: Joakim
full_name: Hellner, Joakim
last_name: Hellner
- first_name: Caterina
full_name: Giannini, Caterina
id: e3fdddd5-f6e0-11ea-865d-ca99ee6367f4
last_name: Giannini
- first_name: Xiangyu
full_name: Xu, Xiangyu
last_name: Xu
- first_name: Jarne
full_name: Pauwels, Jarne
last_name: Pauwels
- first_name: Qian
full_name: Ma, Qian
last_name: Ma
- first_name: Wim
full_name: Dejonghe, Wim
last_name: Dejonghe
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
- first_name: Brigitte
full_name: Van De Cotte, Brigitte
last_name: Van De Cotte
- first_name: Francis
full_name: Impens, Francis
last_name: Impens
- first_name: Kris
full_name: Gevaert, Kris
last_name: Gevaert
- first_name: Ive
full_name: De Smet, Ive
last_name: De Smet
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Daniel Martinez
full_name: Molina, Daniel Martinez
last_name: Molina
- first_name: Eugenia
full_name: Russinova, Eugenia
last_name: Russinova
citation:
ama: Lu Q, Zhang Y, Hellner J, et al. Proteome-wide cellular thermal shift assay
reveals unexpected cross-talk between brassinosteroid and auxin signaling. Proceedings
of the National Academy of Sciences of the United States of America. 2022;119(11).
doi:10.1073/pnas.2118220119
apa: Lu, Q., Zhang, Y., Hellner, J., Giannini, C., Xu, X., Pauwels, J., … Russinova,
E. (2022). Proteome-wide cellular thermal shift assay reveals unexpected cross-talk
between brassinosteroid and auxin signaling. Proceedings of the National Academy
of Sciences of the United States of America. Proceedings of the National Academy
of Sciences. https://doi.org/10.1073/pnas.2118220119
chicago: Lu, Qing, Yonghong Zhang, Joakim Hellner, Caterina Giannini, Xiangyu Xu,
Jarne Pauwels, Qian Ma, et al. “Proteome-Wide Cellular Thermal Shift Assay Reveals Unexpected
Cross-Talk between Brassinosteroid and Auxin Signaling.” Proceedings of the
National Academy of Sciences of the United States of America. Proceedings
of the National Academy of Sciences, 2022. https://doi.org/10.1073/pnas.2118220119.
ieee: Q. Lu et al., “Proteome-wide cellular thermal shift assay reveals unexpected
cross-talk between brassinosteroid and auxin signaling,” Proceedings of the
National Academy of Sciences of the United States of America, vol. 119, no.
11. Proceedings of the National Academy of Sciences, 2022.
ista: Lu Q, Zhang Y, Hellner J, Giannini C, Xu X, Pauwels J, Ma Q, Dejonghe W, Han
H, Van De Cotte B, Impens F, Gevaert K, De Smet I, Friml J, Molina DM, Russinova
E. 2022. Proteome-wide cellular thermal shift assay reveals unexpected cross-talk
between brassinosteroid and auxin signaling. Proceedings of the National Academy
of Sciences of the United States of America. 119(11), e2118220119.
mla: Lu, Qing, et al. “Proteome-Wide Cellular Thermal Shift Assay Reveals Unexpected
Cross-Talk between Brassinosteroid and Auxin Signaling.” Proceedings of the
National Academy of Sciences of the United States of America, vol. 119, no.
11, e2118220119, Proceedings of the National Academy of Sciences, 2022, doi:10.1073/pnas.2118220119.
short: Q. Lu, Y. Zhang, J. Hellner, C. Giannini, X. Xu, J. Pauwels, Q. Ma, W. Dejonghe,
H. Han, B. Van De Cotte, F. Impens, K. Gevaert, I. De Smet, J. Friml, D.M. Molina,
E. Russinova, Proceedings of the National Academy of Sciences of the United States
of America 119 (2022).
date_created: 2022-03-20T23:01:39Z
date_published: 2022-03-07T00:00:00Z
date_updated: 2023-08-03T06:06:27Z
day: '07'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1073/pnas.2118220119
external_id:
isi:
- '000771756300008'
pmid:
- '35254915'
file:
- access_level: open_access
checksum: 83e0fea7919570d0b519b41193342571
content_type: application/pdf
creator: dernst
date_created: 2022-03-21T09:19:47Z
date_updated: 2022-03-21T09:19:47Z
file_id: '10910'
file_name: 2022_PNAS_Lu.pdf
file_size: 2169534
relation: main_file
success: 1
file_date_updated: 2022-03-21T09:19:47Z
has_accepted_license: '1'
intvolume: ' 119'
isi: 1
issue: '11'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
publication: Proceedings of the National Academy of Sciences of the United States
of America
publication_identifier:
eissn:
- 1091-6490
publication_status: published
publisher: Proceedings of the National Academy of Sciences
quality_controlled: '1'
scopus_import: '1'
status: public
title: Proteome-wide cellular thermal shift assay reveals unexpected cross-talk between
brassinosteroid and auxin signaling
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 119
year: '2022'
...
---
_id: '11589'
abstract:
- lang: eng
text: Calcium-dependent protein kinases (CPK) are key components of a wide array
of signaling pathways, translating stress and nutrient signaling into the modulation
of cellular processes such as ion transport and transcription. However, not much
is known about CPKs in endomembrane trafficking. Here, we screened for CPKs that
impact on root growth and gravitropism, by overexpressing constitutively active
forms of CPKs under the control of an inducible promoter in Arabidopsis thaliana.
We found that inducible overexpression of an constitutive active CPK30 (CA-CPK30)
resulted in a loss of root gravitropism and ectopic auxin accumulation in the
root tip. Immunolocalization revealed that CA-CPK30 roots have reduced PIN protein
levels, PIN1 polarity defects and impaired Brefeldin A (BFA)-sensitive trafficking.
Moreover, FM4-64 uptake was reduced, indicative of a defect in endocytosis. The
effects on BFA-sensitive trafficking were not specific to PINs, as BFA could not
induce aggregation of ARF1- and CHC-labeled endosomes in CA-CPK30. Interestingly,
the interference with BFA-body formation, could be reverted by increasing the
extracellular pH, indicating a pH-dependence of this CA-CPK30 effect. Altogether,
our data reveal an important role for CPK30 in root growth regulation and endomembrane
trafficking in Arabidopsis thaliana.
acknowledgement: "RW and JC predoctoral fellows that were supported by the Chinese
Science Counsil. The IPS2 benefits from the support of the LabEx Saclay Plant Sciences-SPS
(ANR-10-LABX-0040-SPS).\r\nWe thank Jen Sheen for establishing and generously sharing
the CKP family clone sets, and for providing useful feedback on the manuscript."
article_number: '862398'
article_processing_charge: No
article_type: original
author:
- first_name: Ren
full_name: Wang, Ren
last_name: Wang
- first_name: Ellie
full_name: Himschoot, Ellie
last_name: Himschoot
- first_name: Jian
full_name: Chen, Jian
last_name: Chen
- first_name: Marie
full_name: Boudsocq, Marie
last_name: Boudsocq
- first_name: Danny
full_name: Geelen, Danny
last_name: Geelen
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Tom
full_name: Beeckman, Tom
last_name: Beeckman
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
citation:
ama: Wang R, Himschoot E, Chen J, et al. Constitutive active CPK30 interferes with
root growth and endomembrane trafficking in Arabidopsis thaliana. Frontiers
in Plant Science. 2022;13. doi:10.3389/fpls.2022.862398
apa: Wang, R., Himschoot, E., Chen, J., Boudsocq, M., Geelen, D., Friml, J., … Vanneste,
S. (2022). Constitutive active CPK30 interferes with root growth and endomembrane
trafficking in Arabidopsis thaliana. Frontiers in Plant Science. Frontiers.
https://doi.org/10.3389/fpls.2022.862398
chicago: Wang, Ren, Ellie Himschoot, Jian Chen, Marie Boudsocq, Danny Geelen, Jiří
Friml, Tom Beeckman, and Steffen Vanneste. “Constitutive Active CPK30 Interferes
with Root Growth and Endomembrane Trafficking in Arabidopsis Thaliana.” Frontiers
in Plant Science. Frontiers, 2022. https://doi.org/10.3389/fpls.2022.862398.
ieee: R. Wang et al., “Constitutive active CPK30 interferes with root growth
and endomembrane trafficking in Arabidopsis thaliana,” Frontiers in Plant Science,
vol. 13. Frontiers, 2022.
ista: Wang R, Himschoot E, Chen J, Boudsocq M, Geelen D, Friml J, Beeckman T, Vanneste
S. 2022. Constitutive active CPK30 interferes with root growth and endomembrane
trafficking in Arabidopsis thaliana. Frontiers in Plant Science. 13, 862398.
mla: Wang, Ren, et al. “Constitutive Active CPK30 Interferes with Root Growth and
Endomembrane Trafficking in Arabidopsis Thaliana.” Frontiers in Plant Science,
vol. 13, 862398, Frontiers, 2022, doi:10.3389/fpls.2022.862398.
short: R. Wang, E. Himschoot, J. Chen, M. Boudsocq, D. Geelen, J. Friml, T. Beeckman,
S. Vanneste, Frontiers in Plant Science 13 (2022).
date_created: 2022-07-17T22:01:54Z
date_published: 2022-06-16T00:00:00Z
date_updated: 2023-08-03T12:01:47Z
day: '16'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.3389/fpls.2022.862398
external_id:
isi:
- '000819250500001'
pmid:
- '35783951'
file:
- access_level: open_access
checksum: 95313515637c0f84de591d204375d764
content_type: application/pdf
creator: dernst
date_created: 2022-07-18T08:05:15Z
date_updated: 2022-07-18T08:05:15Z
file_id: '11596'
file_name: 2022_FrontiersPlantScience_Wang.pdf
file_size: 5040638
relation: main_file
success: 1
file_date_updated: 2022-07-18T08:05:15Z
has_accepted_license: '1'
intvolume: ' 13'
isi: 1
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
pmid: 1
publication: Frontiers in Plant Science
publication_identifier:
eissn:
- 1664-462X
publication_status: published
publisher: Frontiers
quality_controlled: '1'
related_material:
link:
- relation: erratum
url: https://doi.org/10.3389/fpls.2022.1100792
scopus_import: '1'
status: public
title: Constitutive active CPK30 interferes with root growth and endomembrane trafficking
in Arabidopsis thaliana
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 13
year: '2022'
...
---
_id: '11723'
abstract:
- lang: eng
text: Plant cell growth responds rapidly to various stimuli, adapting architecture
to environmental changes. Two major endogenous signals regulating growth are the
phytohormone auxin and the secreted peptides rapid alkalinization factors (RALFs).
Both trigger very rapid cellular responses and also exert long-term effects [Du
et al., Annu. Rev. Plant Biol. 71, 379–402 (2020); Blackburn et al., Plant Physiol.
182, 1657–1666 (2020)]. However, the way, in which these distinct signaling pathways
converge to regulate growth, remains unknown. Here, using vertical confocal microscopy
combined with a microfluidic chip, we addressed the mechanism of RALF action on
growth. We observed correlation between RALF1-induced rapid Arabidopsis thaliana
root growth inhibition and apoplast alkalinization during the initial phase of
the response, and revealed that RALF1 reversibly inhibits primary root growth
through apoplast alkalinization faster than within 1 min. This rapid apoplast
alkalinization was the result of RALF1-induced net H+ influx and was mediated
by the receptor FERONIA (FER). Furthermore, we investigated the cross-talk between
RALF1 and the auxin signaling pathways during root growth regulation. The results
showed that RALF-FER signaling triggered auxin signaling with a delay of approximately
1 h by up-regulating auxin biosynthesis, thus contributing to sustained RALF1-induced
growth inhibition. This biphasic RALF1 action on growth allows plants to respond
rapidly to environmental stimuli and also reprogram growth and development in
the long term.
acknowledgement: We thank Sarah M. Assmann, Kris Vissenberg, and Nadine Paris for
kindly sharing seeds; Matyáš Fendrych for initiating this project and providing
constant support; Lukas Fiedler for revising the manuscript; and Huibin Han and
Arseny Savin for contributing to genotyping. This work was supported by the Austrian
Science Fund (FWF) I 3630-B25 (to J.F.) and the Doctoral Fellowship Progrmme of
the Austrian Academy of Sciences (to L.L.) We also acknowledge Taif University Researchers
Supporting Project TURSP-HC2021/02 and funding “Plants as a tool for sustainable
global development (no. CZ.02.1.01/0.0/0.0/16_019/0000827).”
article_number: e2121058119
article_processing_charge: No
article_type: original
author:
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Huihuang
full_name: Chen, Huihuang
id: 83c96512-15b2-11ec-abd3-b7eede36184f
last_name: Chen
- first_name: Saqer S.
full_name: Alotaibi, Saqer S.
last_name: Alotaibi
- first_name: Aleš
full_name: Pěnčík, Aleš
last_name: Pěnčík
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Ondřej
full_name: Novák, Ondřej
last_name: Novák
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Li L, Chen H, Alotaibi SS, et al. RALF1 peptide triggers biphasic root growth
inhibition upstream of auxin biosynthesis. Proceedings of the National Academy
of Sciences. 2022;119(31). doi:10.1073/pnas.2121058119
apa: Li, L., Chen, H., Alotaibi, S. S., Pěnčík, A., Adamowski, M., Novák, O., &
Friml, J. (2022). RALF1 peptide triggers biphasic root growth inhibition upstream
of auxin biosynthesis. Proceedings of the National Academy of Sciences.
Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.2121058119
chicago: Li, Lanxin, Huihuang Chen, Saqer S. Alotaibi, Aleš Pěnčík, Maciek Adamowski,
Ondřej Novák, and Jiří Friml. “RALF1 Peptide Triggers Biphasic Root Growth Inhibition
Upstream of Auxin Biosynthesis.” Proceedings of the National Academy of Sciences.
Proceedings of the National Academy of Sciences, 2022. https://doi.org/10.1073/pnas.2121058119.
ieee: L. Li et al., “RALF1 peptide triggers biphasic root growth inhibition
upstream of auxin biosynthesis,” Proceedings of the National Academy of Sciences,
vol. 119, no. 31. Proceedings of the National Academy of Sciences, 2022.
ista: Li L, Chen H, Alotaibi SS, Pěnčík A, Adamowski M, Novák O, Friml J. 2022.
RALF1 peptide triggers biphasic root growth inhibition upstream of auxin biosynthesis.
Proceedings of the National Academy of Sciences. 119(31), e2121058119.
mla: Li, Lanxin, et al. “RALF1 Peptide Triggers Biphasic Root Growth Inhibition
Upstream of Auxin Biosynthesis.” Proceedings of the National Academy of Sciences,
vol. 119, no. 31, e2121058119, Proceedings of the National Academy of Sciences,
2022, doi:10.1073/pnas.2121058119.
short: L. Li, H. Chen, S.S. Alotaibi, A. Pěnčík, M. Adamowski, O. Novák, J. Friml,
Proceedings of the National Academy of Sciences 119 (2022).
date_created: 2022-08-04T20:06:49Z
date_published: 2022-07-25T00:00:00Z
date_updated: 2023-08-03T12:43:53Z
day: '25'
ddc:
- '580'
department:
- _id: GradSch
- _id: JiFr
doi: 10.1073/pnas.2121058119
external_id:
isi:
- '000881496900002'
pmid:
- '35878023'
file:
- access_level: open_access
checksum: ae6f19b0d9efba6687f9e4dc1bab1d6e
content_type: application/pdf
creator: dernst
date_created: 2022-08-08T07:42:09Z
date_updated: 2022-08-08T07:42:09Z
file_id: '11747'
file_name: 2022_PNAS_Li.pdf
file_size: 2506262
relation: main_file
success: 1
file_date_updated: 2022-08-08T07:42:09Z
has_accepted_license: '1'
intvolume: ' 119'
isi: 1
issue: '31'
keyword:
- Multidisciplinary
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 26B4D67E-B435-11E9-9278-68D0E5697425
grant_number: '25351'
name: 'A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated
Rapid Growth Inhibition in Arabidopsis Root'
publication: Proceedings of the National Academy of Sciences
publication_identifier:
eissn:
- 1091-6490
issn:
- 0027-8424
publication_status: published
publisher: Proceedings of the National Academy of Sciences
quality_controlled: '1'
scopus_import: '1'
status: public
title: RALF1 peptide triggers biphasic root growth inhibition upstream of auxin biosynthesis
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 119
year: '2022'
...
---
_id: '12053'
abstract:
- lang: eng
text: Strigolactones (SLs) are a class of phytohormones that regulate plant shoot
branching and adventitious root development. However, little is known regarding
the role of SLs in controlling the behavior of the smallest unit of the organism,
the single cell. Here, taking advantage of a classic single-cell model offered
by the cotton (Gossypium hirsutum) fiber cell, we show that SLs, whose biosynthesis
is fine-tuned by gibberellins (GAs), positively regulate cell elongation and cell
wall thickness by promoting the biosynthesis of very-long-chain fatty acids (VLCFAs)
and cellulose, respectively. Furthermore, we identified two layers of transcription
factors (TFs) involved in the hierarchical regulation of this GA-SL crosstalk.
The top-layer TF GROWTH-REGULATING FACTOR 4 (GhGRF4) directly activates expression
of the SL biosynthetic gene DWARF27 (D27) to increase SL accumulation in fiber
cells and GAs induce GhGRF4 expression. SLs induce the expression of four second-layer
TF genes (GhNAC100-2, GhBLH51, GhGT2, and GhB9SHZ1), which transmit SL signals
downstream to two ketoacyl-CoA synthase genes (KCS) and three cellulose synthase
(CesA) genes by directly activating their transcription. Finally, the KCS and
CesA enzymes catalyze the biosynthesis of very long chain fatty acids and cellulose,
respectively, to regulate development of high-grade cotton fibers. In addition
to providing a theoretical basis for cotton fiber improvement, our results shed
light on SL signaling in plant development at the single-cell level.
acknowledgement: This work was supported by the National Natural Science Foundation
of China (32070549), Shaanxi Youth Entrusted Talent Program (20190205), Fundamental
Research Funds for the Central Universities (GK202002005 and GK202201017), Young
Elite Scientists Sponsorship Program by China Association for Science and Technology
(CAST) (2019-2021QNRC001), State Key Laboratory of Cotton Biology Open Fund (CB2020A12
and CB2021A21) and FWF Stand-alone Project (P29988).
article_processing_charge: No
article_type: original
author:
- first_name: Z
full_name: Tian, Z
last_name: Tian
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: L
full_name: Zhu, L
last_name: Zhu
- first_name: B
full_name: Jiang, B
last_name: Jiang
- first_name: H
full_name: Wang, H
last_name: Wang
- first_name: R
full_name: Gao, R
last_name: Gao
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: G
full_name: Xiao, G
last_name: Xiao
citation:
ama: Tian Z, Zhang Y, Zhu L, et al. Strigolactones act downstream of gibberellins
to regulate fiber cell elongation and cell wall thickness in cotton (Gossypium
hirsutum). The Plant Cell. 2022;34(12):4816-4839. doi:10.1093/plcell/koac270
apa: Tian, Z., Zhang, Y., Zhu, L., Jiang, B., Wang, H., Gao, R., … Xiao, G. (2022).
Strigolactones act downstream of gibberellins to regulate fiber cell elongation
and cell wall thickness in cotton (Gossypium hirsutum). The Plant Cell.
Oxford University Press. https://doi.org/10.1093/plcell/koac270
chicago: Tian, Z, Yuzhou Zhang, L Zhu, B Jiang, H Wang, R Gao, Jiří Friml, and G
Xiao. “Strigolactones Act Downstream of Gibberellins to Regulate Fiber Cell Elongation
and Cell Wall Thickness in Cotton (Gossypium Hirsutum).” The Plant Cell.
Oxford University Press, 2022. https://doi.org/10.1093/plcell/koac270.
ieee: Z. Tian et al., “Strigolactones act downstream of gibberellins to regulate
fiber cell elongation and cell wall thickness in cotton (Gossypium hirsutum),”
The Plant Cell, vol. 34, no. 12. Oxford University Press, pp. 4816–4839,
2022.
ista: Tian Z, Zhang Y, Zhu L, Jiang B, Wang H, Gao R, Friml J, Xiao G. 2022. Strigolactones
act downstream of gibberellins to regulate fiber cell elongation and cell wall
thickness in cotton (Gossypium hirsutum). The Plant Cell. 34(12), 4816–4839.
mla: Tian, Z., et al. “Strigolactones Act Downstream of Gibberellins to Regulate
Fiber Cell Elongation and Cell Wall Thickness in Cotton (Gossypium Hirsutum).”
The Plant Cell, vol. 34, no. 12, Oxford University Press, 2022, pp. 4816–39,
doi:10.1093/plcell/koac270.
short: Z. Tian, Y. Zhang, L. Zhu, B. Jiang, H. Wang, R. Gao, J. Friml, G. Xiao,
The Plant Cell 34 (2022) 4816–4839.
date_created: 2022-09-07T14:19:39Z
date_published: 2022-12-01T00:00:00Z
date_updated: 2023-08-03T13:41:06Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1093/plcell/koac270
external_id:
isi:
- '000852753000001'
pmid:
- '36040191'
file:
- access_level: open_access
checksum: 1c606d9545f29dfca15235f69ad27b58
content_type: application/pdf
creator: dernst
date_created: 2023-01-20T08:29:12Z
date_updated: 2023-01-20T08:29:12Z
file_id: '12318'
file_name: 2022_PlantCell_Tian.pdf
file_size: 3282540
relation: main_file
success: 1
file_date_updated: 2023-01-20T08:29:12Z
has_accepted_license: '1'
intvolume: ' 34'
isi: 1
issue: '12'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
page: 4816-4839
pmid: 1
project:
- _id: 262EF96E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29988
name: RNA-directed DNA methylation in plant development
publication: The Plant Cell
publication_identifier:
eissn:
- 1532-298X
issn:
- 1040-4651
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
related_material:
link:
- relation: erratum
url: https://doi.org/10.1093/plcell/koac342
scopus_import: '1'
status: public
title: Strigolactones act downstream of gibberellins to regulate fiber cell elongation
and cell wall thickness in cotton (Gossypium hirsutum)
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 34
year: '2022'
...
---
_id: '12052'
abstract:
- lang: eng
text: Directionality in the intercellular transport of the plant hormone auxin is
determined by polar plasma membrane localization of PIN-FORMED (PIN) auxin transport
proteins. However, apart from PIN phosphorylation at conserved motifs, no further
determinants explicitly controlling polar PIN sorting decisions have been identified.
Here we present Arabidopsis WAVY GROWTH 3 (WAV3) and closely related RING-finger
E3 ubiquitin ligases, whose loss-of-function mutants show a striking apical-to-basal
polarity switch in PIN2 localization in root meristem cells. WAV3 E3 ligases function
as essential determinants for PIN polarity, acting independently from PINOID/WAG-dependent
PIN phosphorylation. They antagonize ectopic deposition of de novo synthesized
PIN proteins already immediately following completion of cell division, presumably
via preventing PIN sorting into basal, ARF GEF-mediated trafficking. Our findings
reveal an involvement of E3 ligases in the selective targeting of apically localized
PINs in higher plants.
acknowledgement: We would like to thank Tatsuo Sakai, Marcus Heisler, Toru Fujiwara,
Lucia Strader, Christian Hardtke, Malcolm Bennett, Claus Schwechheimer, Gerd Jürgens
and Remko Offringa for sharing published materials and Alba Grau Gimeno for support.
We are greatly indebted to Bert de Rybel for supporting N.K. and M.G. to work on
the final stages of manuscript preparation as postdocs in his laboratory. A full-length
SOR1 cDNA clone (J090099M14) was obtained from the National Agriculture and Food
Research Organization (NARO, Japan). Support by the Multiscale Imaging Core Facility
at the BOKU is greatly acknowledged. This work has been supported by grants from
the Austrian Science Fund (FWF P25931-B16; P31493-B25 to Christian Luschnig; I3630-B25
to Jiří Friml; P30850-B32 to Barbara Korbei) and from the Swiss National Funds (31003A-165877/1
to Markus Geisler) and the European Union’s Horizon 2020 research and innovation
program (Marie Skłodowska-Curie grant agreement No 885979 to Matouš Glanc).
article_number: '5147'
article_processing_charge: No
article_type: original
author:
- first_name: N
full_name: Konstantinova, N
last_name: Konstantinova
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: R
full_name: Keshkeih, R
last_name: Keshkeih
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: M
full_name: Di Donato, M
last_name: Di Donato
- first_name: K
full_name: Retzer, K
last_name: Retzer
- first_name: J
full_name: Moulinier-Anzola, J
last_name: Moulinier-Anzola
- first_name: M
full_name: Schwihla, M
last_name: Schwihla
- first_name: B
full_name: Korbei, B
last_name: Korbei
- first_name: M
full_name: Geisler, M
last_name: Geisler
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: C
full_name: Luschnig, C
last_name: Luschnig
citation:
ama: Konstantinova N, Hörmayer L, Glanc M, et al. WAVY GROWTH Arabidopsis E3 ubiquitin
ligases affect apical PIN sorting decisions. Nature Communications. 2022;13.
doi:10.1038/s41467-022-32888-8
apa: Konstantinova, N., Hörmayer, L., Glanc, M., Keshkeih, R., Tan, S., Di Donato,
M., … Luschnig, C. (2022). WAVY GROWTH Arabidopsis E3 ubiquitin ligases affect
apical PIN sorting decisions. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-022-32888-8
chicago: Konstantinova, N, Lukas Hörmayer, Matous Glanc, R Keshkeih, Shutang Tan,
M Di Donato, K Retzer, et al. “WAVY GROWTH Arabidopsis E3 Ubiquitin Ligases Affect
Apical PIN Sorting Decisions.” Nature Communications. Springer Nature,
2022. https://doi.org/10.1038/s41467-022-32888-8.
ieee: N. Konstantinova et al., “WAVY GROWTH Arabidopsis E3 ubiquitin ligases
affect apical PIN sorting decisions,” Nature Communications, vol. 13. Springer
Nature, 2022.
ista: Konstantinova N, Hörmayer L, Glanc M, Keshkeih R, Tan S, Di Donato M, Retzer
K, Moulinier-Anzola J, Schwihla M, Korbei B, Geisler M, Friml J, Luschnig C. 2022.
WAVY GROWTH Arabidopsis E3 ubiquitin ligases affect apical PIN sorting decisions.
Nature Communications. 13, 5147.
mla: Konstantinova, N., et al. “WAVY GROWTH Arabidopsis E3 Ubiquitin Ligases Affect
Apical PIN Sorting Decisions.” Nature Communications, vol. 13, 5147, Springer
Nature, 2022, doi:10.1038/s41467-022-32888-8.
short: N. Konstantinova, L. Hörmayer, M. Glanc, R. Keshkeih, S. Tan, M. Di Donato,
K. Retzer, J. Moulinier-Anzola, M. Schwihla, B. Korbei, M. Geisler, J. Friml,
C. Luschnig, Nature Communications 13 (2022).
date_created: 2022-09-07T14:19:26Z
date_published: 2022-09-01T00:00:00Z
date_updated: 2023-08-03T13:40:32Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41467-022-32888-8
external_id:
isi:
- '000848744900004'
pmid:
- '36050482'
file:
- access_level: open_access
checksum: 43336758c89cd6c045839089af070afe
content_type: application/pdf
creator: dernst
date_created: 2022-09-08T07:46:16Z
date_updated: 2022-09-08T07:46:16Z
file_id: '12063'
file_name: 2022_NatureCommunications_Konstantinova.pdf
file_size: 6678579
relation: main_file
success: 1
file_date_updated: 2022-09-08T07:46:16Z
has_accepted_license: '1'
intvolume: ' 13'
isi: 1
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: Nature Communications
publication_identifier:
issn:
- 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- relation: erratum
url: https://doi.org/10.1038/s41467-022-33198-9
status: public
title: WAVY GROWTH Arabidopsis E3 ubiquitin ligases affect apical PIN sorting decisions
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 13
year: '2022'
...
---
_id: '12054'
abstract:
- lang: eng
text: 'Polar auxin transport is unique to plants and coordinates their growth and
development1,2. The PIN-FORMED (PIN) auxin transporters exhibit highly asymmetrical
localizations at the plasma membrane and drive polar auxin transport3,4; however,
their structures and transport mechanisms remain largely unknown. Here, we report
three inward-facing conformation structures of Arabidopsis thaliana PIN1: the
apo state, bound to the natural auxin indole-3-acetic acid (IAA), and in complex
with the polar auxin transport inhibitor N-1-naphthylphthalamic acid (NPA). The
transmembrane domain of PIN1 shares a conserved NhaA fold5. In the substrate-bound
structure, IAA is coordinated by both hydrophobic stacking and hydrogen bonding.
NPA competes with IAA for the same site at the intracellular pocket, but with
a much higher affinity. These findings inform our understanding of the substrate
recognition and transport mechanisms of PINs and set up a framework for future
research on directional auxin transport, one of the most crucial processes underlying
plant development.'
acknowledgement: We thank the Cryo-EM Center of the University of Science and Technology
of China (USTC) and the Center for Biological Imaging (CBI), Institute of Biophysics,
Chinese Academy of Science, for the EM facility support; we thank B. Zhu, X. Huang
and all the other staff members for their technical support on cryo-EM data collection.
We thank J. Ren for his technical support with the transport assays and M. Seeger
for providing the sybody libraries. This work was supported by the Strategic Priority
Research Program of Chinese Academy of Sciences (XDB 37020204 to D.L. and XDB37020103
to Linfeng Sun), National Natural Science Foundation of China (82151215 and 31870726
to D.L., 31900885 to X.L., and 31870732 to Linfeng Sun), Natural Science Foundation
of Anhui Province (2008085MC90 to X.L. and 2008085J15 to Linfeng Sun), the Fundamental
Research Funds for the Central Universities (WK9100000031 to Linfeng Sun), and the
USTC Research Funds of the Double First-Class Initiative (YD9100002004 to Linfeng
Sun). Linfeng Sun is supported by an Outstanding Young Scholar Award from the Qiu
Shi Science and Technologies Foundation, and a Young Scholar Award from the Cyrus
Tang Foundation.
article_processing_charge: No
article_type: original
author:
- first_name: Z
full_name: Yang, Z
last_name: Yang
- first_name: J
full_name: Xia, J
last_name: Xia
- first_name: J
full_name: Hong, J
last_name: Hong
- first_name: C
full_name: Zhang, C
last_name: Zhang
- first_name: H
full_name: Wei, H
last_name: Wei
- first_name: W
full_name: Ying, W
last_name: Ying
- first_name: C
full_name: Sun, C
last_name: Sun
- first_name: L
full_name: Sun, L
last_name: Sun
- first_name: Y
full_name: Mao, Y
last_name: Mao
- first_name: Y
full_name: Gao, Y
last_name: Gao
- first_name: S
full_name: Tan, S
last_name: Tan
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: D
full_name: Li, D
last_name: Li
- first_name: X
full_name: Liu, X
last_name: Liu
- first_name: L
full_name: Sun, L
last_name: Sun
citation:
ama: Yang Z, Xia J, Hong J, et al. Structural insights into auxin recognition and
efflux by Arabidopsis PIN1. Nature. 2022;609(7927):611-615. doi:10.1038/s41586-022-05143-9
apa: Yang, Z., Xia, J., Hong, J., Zhang, C., Wei, H., Ying, W., … Sun, L. (2022).
Structural insights into auxin recognition and efflux by Arabidopsis PIN1. Nature.
Springer Nature. https://doi.org/10.1038/s41586-022-05143-9
chicago: Yang, Z, J Xia, J Hong, C Zhang, H Wei, W Ying, C Sun, et al. “Structural
Insights into Auxin Recognition and Efflux by Arabidopsis PIN1.” Nature.
Springer Nature, 2022. https://doi.org/10.1038/s41586-022-05143-9.
ieee: Z. Yang et al., “Structural insights into auxin recognition and efflux
by Arabidopsis PIN1,” Nature, vol. 609, no. 7927. Springer Nature, pp.
611–615, 2022.
ista: Yang Z, Xia J, Hong J, Zhang C, Wei H, Ying W, Sun C, Sun L, Mao Y, Gao Y,
Tan S, Friml J, Li D, Liu X, Sun L. 2022. Structural insights into auxin recognition
and efflux by Arabidopsis PIN1. Nature. 609(7927), 611–615.
mla: Yang, Z., et al. “Structural Insights into Auxin Recognition and Efflux by
Arabidopsis PIN1.” Nature, vol. 609, no. 7927, Springer Nature, 2022, pp.
611–15, doi:10.1038/s41586-022-05143-9.
short: Z. Yang, J. Xia, J. Hong, C. Zhang, H. Wei, W. Ying, C. Sun, L. Sun, Y. Mao,
Y. Gao, S. Tan, J. Friml, D. Li, X. Liu, L. Sun, Nature 609 (2022) 611–615.
date_created: 2022-09-07T14:19:52Z
date_published: 2022-08-02T00:00:00Z
date_updated: 2023-08-03T13:41:44Z
day: '02'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41586-022-05143-9
external_id:
isi:
- '000848082900002'
pmid:
- '35917925'
file:
- access_level: open_access
checksum: 3136a585f8e1c7e73b5e1418b3d01898
content_type: application/pdf
creator: dernst
date_created: 2022-09-08T08:02:54Z
date_updated: 2022-09-08T08:02:54Z
file_id: '12064'
file_name: 2022_Nature_Yang.pdf
file_size: 32344580
relation: main_file
success: 1
file_date_updated: 2022-09-08T08:02:54Z
has_accepted_license: '1'
intvolume: ' 609'
isi: 1
issue: '7927'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
page: 611-615
pmid: 1
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Structural insights into auxin recognition and efflux by Arabidopsis PIN1
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 609
year: '2022'
...
---
_id: '12121'
abstract:
- lang: eng
text: Autophagosomes are double-membraned vesicles that traffic harmful or unwanted
cellular macromolecules to the vacuole for recycling. Although autophagosome biogenesis
has been extensively studied, autophagosome maturation, i.e., delivery and fusion
with the vacuole, remains largely unknown in plants. Here, we have identified
an autophagy adaptor, CFS1, that directly interacts with the autophagosome marker
ATG8 and localizes on both membranes of the autophagosome. Autophagosomes form
normally in Arabidopsis thaliana cfs1 mutants, but their delivery to the vacuole
is disrupted. CFS1’s function is evolutionarily conserved in plants, as it also
localizes to the autophagosomes and plays a role in autophagic flux in the liverwort
Marchantia polymorpha. CFS1 regulates autophagic flux by bridging autophagosomes
with the multivesicular body-localized ESCRT-I component VPS23A, leading to the
formation of amphisomes. Similar to CFS1-ATG8 interaction, disrupting the CFS1-VPS23A
interaction blocks autophagic flux and renders plants sensitive to nitrogen starvation.
Altogether, our results reveal a conserved vacuolar sorting hub that regulates
autophagic flux in plants.
acknowledgement: "We thank Suayip Ustün, Karin Schumacher, Erika Isono, Gerd Juergens,
Takashi Ueda, Daniel Hofius, and Liwen Jiang for sharing published materials.\r\nWe
acknowledge funding from Austrian Academy of Sciences, Austrian Science Fund (FWF,
P 32355, P 34944), Austrian Science Fund (FWF-SFB F79), Vienna Science and Technology\r\nFund
(WWTF, LS17-047) to Y. Dagdas; Austrian Academy of Sciences DOC Fellowship to J.
Zhao, Marie Curie VIP2 Fellowship to J.C. De La Concepcion and M. Clavel; Hong Kong
Research Grant Council (GRF14121019, 14113921, AoE/M-05/12, C4002-17G) to B.-H.
Kang. We thank Vienna Biocenter Core Facilities (VBCF) Protein Chemistry, Biooptics,
Plant Sciences, Molecular Biology, and Protein Technologies. We thank J. Matthew
Watson\r\nand members of the Dagdas lab for the critical reading and editing of
the manuscript."
article_number: e202203139
article_processing_charge: No
article_type: original
author:
- first_name: Jierui
full_name: Zhao, Jierui
last_name: Zhao
- first_name: Mai Thu
full_name: Bui, Mai Thu
last_name: Bui
- first_name: Juncai
full_name: Ma, Juncai
last_name: Ma
- first_name: Fabian
full_name: Künzl, Fabian
last_name: Künzl
- first_name: Lorenzo
full_name: Picchianti, Lorenzo
last_name: Picchianti
- first_name: Juan Carlos
full_name: De La Concepcion, Juan Carlos
last_name: De La Concepcion
- first_name: Yixuan
full_name: Chen, Yixuan
last_name: Chen
- first_name: Sofia
full_name: Petsangouraki, Sofia
last_name: Petsangouraki
- first_name: Azadeh
full_name: Mohseni, Azadeh
last_name: Mohseni
- first_name: Marta
full_name: García-Leon, Marta
last_name: García-Leon
- first_name: Marta Salas
full_name: Gomez, Marta Salas
last_name: Gomez
- first_name: Caterina
full_name: Giannini, Caterina
id: e3fdddd5-f6e0-11ea-865d-ca99ee6367f4
last_name: Giannini
- first_name: Dubois
full_name: Gwennogan, Dubois
last_name: Gwennogan
- first_name: Roksolana
full_name: Kobylinska, Roksolana
last_name: Kobylinska
- first_name: Marion
full_name: Clavel, Marion
last_name: Clavel
- first_name: Swen
full_name: Schellmann, Swen
last_name: Schellmann
- first_name: Yvon
full_name: Jaillais, Yvon
last_name: Jaillais
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Byung-Ho
full_name: Kang, Byung-Ho
last_name: Kang
- first_name: Yasin
full_name: Dagdas, Yasin
last_name: Dagdas
citation:
ama: Zhao J, Bui MT, Ma J, et al. Plant autophagosomes mature into amphisomes prior
to their delivery to the central vacuole. Journal of Cell Biology. 2022;221(12).
doi:10.1083/jcb.202203139
apa: Zhao, J., Bui, M. T., Ma, J., Künzl, F., Picchianti, L., De La Concepcion,
J. C., … Dagdas, Y. (2022). Plant autophagosomes mature into amphisomes prior
to their delivery to the central vacuole. Journal of Cell Biology. Rockefeller
University Press. https://doi.org/10.1083/jcb.202203139
chicago: Zhao, Jierui, Mai Thu Bui, Juncai Ma, Fabian Künzl, Lorenzo Picchianti,
Juan Carlos De La Concepcion, Yixuan Chen, et al. “Plant Autophagosomes Mature
into Amphisomes Prior to Their Delivery to the Central Vacuole.” Journal of
Cell Biology. Rockefeller University Press, 2022. https://doi.org/10.1083/jcb.202203139.
ieee: J. Zhao et al., “Plant autophagosomes mature into amphisomes prior
to their delivery to the central vacuole,” Journal of Cell Biology, vol.
221, no. 12. Rockefeller University Press, 2022.
ista: Zhao J, Bui MT, Ma J, Künzl F, Picchianti L, De La Concepcion JC, Chen Y,
Petsangouraki S, Mohseni A, García-Leon M, Gomez MS, Giannini C, Gwennogan D,
Kobylinska R, Clavel M, Schellmann S, Jaillais Y, Friml J, Kang B-H, Dagdas Y.
2022. Plant autophagosomes mature into amphisomes prior to their delivery to the
central vacuole. Journal of Cell Biology. 221(12), e202203139.
mla: Zhao, Jierui, et al. “Plant Autophagosomes Mature into Amphisomes Prior to
Their Delivery to the Central Vacuole.” Journal of Cell Biology, vol. 221,
no. 12, e202203139, Rockefeller University Press, 2022, doi:10.1083/jcb.202203139.
short: J. Zhao, M.T. Bui, J. Ma, F. Künzl, L. Picchianti, J.C. De La Concepcion,
Y. Chen, S. Petsangouraki, A. Mohseni, M. García-Leon, M.S. Gomez, C. Giannini,
D. Gwennogan, R. Kobylinska, M. Clavel, S. Schellmann, Y. Jaillais, J. Friml,
B.-H. Kang, Y. Dagdas, Journal of Cell Biology 221 (2022).
date_created: 2023-01-12T11:57:10Z
date_published: 2022-12-01T00:00:00Z
date_updated: 2023-08-03T14:20:15Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1083/jcb.202203139
external_id:
isi:
- '000932958800001'
pmid:
- '36260289'
file:
- access_level: open_access
checksum: 050b5cc4b25e6b94fe3e3cbfe0f5c06b
content_type: application/pdf
creator: dernst
date_created: 2023-01-23T10:30:11Z
date_updated: 2023-01-23T10:30:11Z
file_id: '12342'
file_name: 2022_JCB_Zhao.pdf
file_size: 10365777
relation: main_file
success: 1
file_date_updated: 2023-01-23T10:30:11Z
has_accepted_license: '1'
intvolume: ' 221'
isi: 1
issue: '12'
keyword:
- Cell Biology
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
pmid: 1
publication: Journal of Cell Biology
publication_identifier:
eissn:
- 1540-8140
issn:
- 0021-9525
publication_status: published
publisher: Rockefeller University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Plant autophagosomes mature into amphisomes prior to their delivery to the
central vacuole
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 221
year: '2022'
...
---
_id: '12130'
abstract:
- lang: eng
text: Germline determination is essential for species survival and evolution in
multicellular organisms. In most flowering plants, formation of the female germline
is initiated with specification of one megaspore mother cell (MMC) in each ovule;
however, the molecular mechanism underlying this key event remains unclear. Here
we report that spatially restricted auxin signaling promotes MMC fate in Arabidopsis.
Our results show that the microRNA160 (miR160) targeted gene ARF17 (AUXIN RESPONSE
FACTOR17) is required for promoting MMC specification by genetically interacting
with the SPL/NZZ (SPOROCYTELESS/NOZZLE) gene. Alterations of auxin signaling cause
formation of supernumerary MMCs in an ARF17- and SPL/NZZ-dependent manner. Furthermore,
miR160 and ARF17 are indispensable for attaining a normal auxin maximum at the
ovule apex via modulating the expression domain of PIN1 (PIN-FORMED1) auxin transporter.
Our findings elucidate the mechanism by which auxin signaling promotes the acquisition
of female germline cell fate in plants.
acknowledgement: "We thank A. Cheung,W. Lukowitz, V.Walbot, D.Weijers, and R. Yadegari
for critically reading the manuscript; E. Xiong and G. Zhang for preparing some
experiments, T. Schuck, J. Gonnering, and P. Engevold for plant care, the Arabidopsis
Biological Resource Center (ABRC) for ARF10,ARF16, ARF17, EMS1,MIR160a BAC clones
and cDNAs, the SALK_090804 seed, T. Nakagawa for pGBW vectors, Y. Zhao for the YUC1
cDNA, Q. Chen for the pHEE401E vector, R. Yadegari for pAT5G01860::n1GFP, pAT5G45980:n1GFP,
pAT5G50490::n1GFP, pAT5G56200:n1GFP vectors, and D.Weijers for the pGreenII KAN
SV40-3×GFP and R2D2 vectors, W. Yang for the splmutant, Y. Qin for the pKNU::KNU-VENUS
vector and seed, G. Tang for the STTM160/160-48 vector, and L. Colombo for pPIN1::PIN1-GFP
spl and pin1-5 seeds. This work was supported by the US National Science Foundation
(NSF)-Israel Binational Science Foundation (BSF) research grant to D.Z. (IOS-1322796)
and T.A. (2012756). D.Z. also\r\ngratefully acknowledges supports of the Shaw Scientist
Award from the Greater Milwaukee Foundation, USDA National Institute of Food and
Agriculture (NIFA, 2022-67013-36294), the UWM Discovery and Innovation Grant, the
Bradley Catalyst Award from the UWM Research\r\nFoundation, and WiSys and UW System
Applied Research Funding Programs."
article_number: '6960'
article_processing_charge: No
article_type: original
author:
- first_name: Jian
full_name: Huang, Jian
last_name: Huang
- first_name: Lei
full_name: Zhao, Lei
last_name: Zhao
- first_name: Shikha
full_name: Malik, Shikha
last_name: Malik
- first_name: Benjamin R.
full_name: Gentile, Benjamin R.
last_name: Gentile
- first_name: Va
full_name: Xiong, Va
last_name: Xiong
- first_name: Tzahi
full_name: Arazi, Tzahi
last_name: Arazi
- first_name: Heather A.
full_name: Owen, Heather A.
last_name: Owen
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Dazhong
full_name: Zhao, Dazhong
last_name: Zhao
citation:
ama: Huang J, Zhao L, Malik S, et al. Specification of female germline by microRNA
orchestrated auxin signaling in Arabidopsis. Nature Communications. 2022;13.
doi:10.1038/s41467-022-34723-6
apa: Huang, J., Zhao, L., Malik, S., Gentile, B. R., Xiong, V., Arazi, T., … Zhao,
D. (2022). Specification of female germline by microRNA orchestrated auxin signaling
in Arabidopsis. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-022-34723-6
chicago: Huang, Jian, Lei Zhao, Shikha Malik, Benjamin R. Gentile, Va Xiong, Tzahi
Arazi, Heather A. Owen, Jiří Friml, and Dazhong Zhao. “Specification of Female
Germline by MicroRNA Orchestrated Auxin Signaling in Arabidopsis.” Nature Communications.
Springer Nature, 2022. https://doi.org/10.1038/s41467-022-34723-6.
ieee: J. Huang et al., “Specification of female germline by microRNA orchestrated
auxin signaling in Arabidopsis,” Nature Communications, vol. 13. Springer
Nature, 2022.
ista: Huang J, Zhao L, Malik S, Gentile BR, Xiong V, Arazi T, Owen HA, Friml J,
Zhao D. 2022. Specification of female germline by microRNA orchestrated auxin
signaling in Arabidopsis. Nature Communications. 13, 6960.
mla: Huang, Jian, et al. “Specification of Female Germline by MicroRNA Orchestrated
Auxin Signaling in Arabidopsis.” Nature Communications, vol. 13, 6960,
Springer Nature, 2022, doi:10.1038/s41467-022-34723-6.
short: J. Huang, L. Zhao, S. Malik, B.R. Gentile, V. Xiong, T. Arazi, H.A. Owen,
J. Friml, D. Zhao, Nature Communications 13 (2022).
date_created: 2023-01-12T12:02:41Z
date_published: 2022-11-15T00:00:00Z
date_updated: 2023-08-04T08:52:01Z
day: '15'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41467-022-34723-6
external_id:
isi:
- '000884426700001'
pmid:
- '36379956'
file:
- access_level: open_access
checksum: 233922a7b9507d9d48591e6799e4526e
content_type: application/pdf
creator: dernst
date_created: 2023-01-23T11:17:33Z
date_updated: 2023-01-23T11:17:33Z
file_id: '12346'
file_name: 2022_NatureCommunications_Huang.pdf
file_size: 3375249
relation: main_file
success: 1
file_date_updated: 2023-01-23T11:17:33Z
has_accepted_license: '1'
intvolume: ' 13'
isi: 1
keyword:
- General Physics and Astronomy
- General Biochemistry
- Genetics and Molecular Biology
- General Chemistry
- Multidisciplinary
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
publication: Nature Communications
publication_identifier:
issn:
- 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Specification of female germline by microRNA orchestrated auxin signaling in
Arabidopsis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 13
year: '2022'
...
---
_id: '12239'
abstract:
- lang: eng
text: Biological systems are the sum of their dynamic three-dimensional (3D) parts.
Therefore, it is critical to study biological structures in 3D and at high resolution
to gain insights into their physiological functions. Electron microscopy of metal
replicas of unroofed cells and isolated organelles has been a key technique to
visualize intracellular structures at nanometer resolution. However, many of these
methods require specialized equipment and personnel to complete them. Here, we
present novel accessible methods to analyze biological structures in unroofed
cells and biochemically isolated organelles in 3D and at nanometer resolution,
focusing on Arabidopsis clathrin-coated vesicles (CCVs). While CCVs are essential
trafficking organelles, their detailed structural information is lacking due to
their poor preservation when observed via classical electron microscopy protocols
experiments. First, we establish a method to visualize CCVs in unroofed cells
using scanning transmission electron microscopy tomography, providing sufficient
resolution to define the clathrin coat arrangements. Critically, the samples are
prepared directly on electron microscopy grids, removing the requirement to use
extremely corrosive acids, thereby enabling the use of this method in any electron
microscopy lab. Secondly, we demonstrate that this standardized sample preparation
allows the direct comparison of isolated CCV samples with those visualized in
cells. Finally, to facilitate the high-throughput and robust screening of metal
replicated samples, we provide a deep learning analysis method to screen the “pseudo
3D” morphologies of CCVs imaged with 2D modalities. Collectively, our work establishes
accessible ways to examine the 3D structure of biological samples and provide
novel insights into the structure of plant CCVs.
acknowledged_ssus:
- _id: EM-Fac
- _id: LifeSc
- _id: Bio
acknowledgement: A.J. is supported by funding from the Austrian Science Fund I3630B25
(to J.F.). This research was supported by the Scientific Service Units of Institute
of Science and Technology Austria (ISTA) through resources provided by the Electron
Microscopy Facility, Lab Support Facility, and the Imaging and Optics Facility.
We acknowledge Prof. David Robinson (Heidelberg) and Prof. Jan Traas (Lyon) for
making us aware of previously published classical on-grid preparation methods. No
conflict of interest declared.
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Christoph M
full_name: Sommer, Christoph M
id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
last_name: Sommer
orcid: 0000-0003-1216-9105
- first_name: Tommaso
full_name: Costanzo, Tommaso
id: D93824F4-D9BA-11E9-BB12-F207E6697425
last_name: Costanzo
orcid: 0000-0001-9732-3815
- first_name: Dana A.
full_name: Dahhan, Dana A.
last_name: Dahhan
- first_name: Sebastian Y.
full_name: Bednarek, Sebastian Y.
last_name: Bednarek
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Johnson AJ, Kaufmann W, Sommer CM, et al. Three-dimensional visualization of
planta clathrin-coated vesicles at ultrastructural resolution. Molecular Plant.
2022;15(10):1533-1542. doi:10.1016/j.molp.2022.09.003
apa: Johnson, A. J., Kaufmann, W., Sommer, C. M., Costanzo, T., Dahhan, D. A., Bednarek,
S. Y., & Friml, J. (2022). Three-dimensional visualization of planta clathrin-coated
vesicles at ultrastructural resolution. Molecular Plant. Elsevier. https://doi.org/10.1016/j.molp.2022.09.003
chicago: Johnson, Alexander J, Walter Kaufmann, Christoph M Sommer, Tommaso Costanzo,
Dana A. Dahhan, Sebastian Y. Bednarek, and Jiří Friml. “Three-Dimensional Visualization
of Planta Clathrin-Coated Vesicles at Ultrastructural Resolution.” Molecular
Plant. Elsevier, 2022. https://doi.org/10.1016/j.molp.2022.09.003.
ieee: A. J. Johnson et al., “Three-dimensional visualization of planta clathrin-coated
vesicles at ultrastructural resolution,” Molecular Plant, vol. 15, no.
10. Elsevier, pp. 1533–1542, 2022.
ista: Johnson AJ, Kaufmann W, Sommer CM, Costanzo T, Dahhan DA, Bednarek SY, Friml
J. 2022. Three-dimensional visualization of planta clathrin-coated vesicles at
ultrastructural resolution. Molecular Plant. 15(10), 1533–1542.
mla: Johnson, Alexander J., et al. “Three-Dimensional Visualization of Planta Clathrin-Coated
Vesicles at Ultrastructural Resolution.” Molecular Plant, vol. 15, no.
10, Elsevier, 2022, pp. 1533–42, doi:10.1016/j.molp.2022.09.003.
short: A.J. Johnson, W. Kaufmann, C.M. Sommer, T. Costanzo, D.A. Dahhan, S.Y. Bednarek,
J. Friml, Molecular Plant 15 (2022) 1533–1542.
date_created: 2023-01-16T09:51:49Z
date_published: 2022-10-03T00:00:00Z
date_updated: 2023-08-04T09:39:24Z
day: '03'
ddc:
- '580'
department:
- _id: JiFr
- _id: EM-Fac
- _id: Bio
doi: 10.1016/j.molp.2022.09.003
external_id:
isi:
- '000882769800009'
pmid:
- '36081349'
file:
- access_level: open_access
checksum: 04d5c12490052d03e4dc4412338a43dd
content_type: application/pdf
creator: dernst
date_created: 2023-01-30T07:46:51Z
date_updated: 2023-01-30T07:46:51Z
file_id: '12435'
file_name: 2022_MolecularPlant_Johnson.pdf
file_size: 2307251
relation: main_file
success: 1
file_date_updated: 2023-01-30T07:46:51Z
has_accepted_license: '1'
intvolume: ' 15'
isi: 1
issue: '10'
keyword:
- Plant Science
- Molecular Biology
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
page: 1533-1542
pmid: 1
project:
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: Molecular Plant
publication_identifier:
issn:
- 1674-2052
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Three-dimensional visualization of planta clathrin-coated vesicles at ultrastructural
resolution
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 15
year: '2022'
...
---
_id: '11489'
abstract:
- lang: eng
text: Much of plant development depends on cell-to-cell redistribution of the plant
hormone auxin, which is facilitated by the plasma membrane (PM) localized PIN
FORMED (PIN) proteins. Auxin export activity, developmental roles, subcellular
trafficking, and polarity of PINs have been well studied, but their structure
remains elusive besides a rough outline that they contain two groups of 5 alpha-helices
connected by a large hydrophilic loop (HL). Here, we focus on the PIN1 HL as we
could produce it in sufficient quantities for biochemical investigations to provide
insights into its secondary structure. Circular dichroism (CD) studies revealed
its nature as an intrinsically disordered protein (IDP), manifested by the increase
of structure content upon thermal melting. Consistent with IDPs serving as interaction
platforms, PIN1 loops homodimerize. PIN1 HL cytoplasmic overexpression in Arabidopsis
disrupts early endocytic trafficking of PIN1 and PIN2 and causes defects in the
cotyledon vasculature formation. In summary, we demonstrate that PIN1 HL has an
intrinsically disordered nature, which must be considered to gain further structural
insights. Some secondary structures may form transiently during pairing with known
and yet-to-be-discovered interactors.
acknowledgement: 'We thank Charo del Genio from Coventry University and Richard Napier
from the University of Warwick for helpful discussion concerning protein modeling
and inspiration concerning CD spectroscopy, respectively. We thank Jan Hejatko for
sharing the published AHP2 construct. We also thank Josef Houser from the core facility
BIC CEITEC for valuable assistance, discussions, and ideas relating to CD. We acknowledge
the: Core Facility CELLIM of CEITEC supported by the Czech-BioImaging large RI project
(LM2018129 funded by MEYS CR), part of the Euro-BioImaging (www.eurobioimaging.eu
accessed on 1 January 2016) ALM and medical imaging Node (Brno, CZ), CF Biomolecular
Interactions and Crystallization of CIISB, Instruct-CZ Centre, supported by MEYS
CR (LM2018127) and European Regional Development Fund-Project “UP CIISB“ (No. CZ.02.1.01/0.0/0.0/18_046/0015974)
for their support with obtaining scientific data presented in this paper; Plant
Sciences Core Facility of CEITEC Masaryk University for technical support. Open
Access Funding by the Austrian Science Fund (FWF).'
article_processing_charge: Yes
article_type: original
author:
- first_name: V
full_name: Bilanovičová, V
last_name: Bilanovičová
- first_name: N
full_name: Rýdza, N
last_name: Rýdza
- first_name: L
full_name: Koczka, L
last_name: Koczka
- first_name: M
full_name: Hess, M
last_name: Hess
- first_name: E
full_name: Feraru, E
last_name: Feraru
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: T
full_name: Nodzyński, T
last_name: Nodzyński
citation:
ama: Bilanovičová V, Rýdza N, Koczka L, et al. The hydrophilic loop of Arabidopsis
PIN1 auxin efflux carrier harbors hallmarks of an intrinsically disordered protein.
International Journal of Molecular Sciences. 2022;23(11):6352. doi:10.3390/ijms23116352
apa: Bilanovičová, V., Rýdza, N., Koczka, L., Hess, M., Feraru, E., Friml, J., &
Nodzyński, T. (2022). The hydrophilic loop of Arabidopsis PIN1 auxin efflux carrier
harbors hallmarks of an intrinsically disordered protein. International Journal
of Molecular Sciences. MDPI. https://doi.org/10.3390/ijms23116352
chicago: Bilanovičová, V, N Rýdza, L Koczka, M Hess, E Feraru, Jiří Friml, and T
Nodzyński. “The Hydrophilic Loop of Arabidopsis PIN1 Auxin Efflux Carrier Harbors
Hallmarks of an Intrinsically Disordered Protein.” International Journal of
Molecular Sciences. MDPI, 2022. https://doi.org/10.3390/ijms23116352.
ieee: V. Bilanovičová et al., “The hydrophilic loop of Arabidopsis PIN1 auxin
efflux carrier harbors hallmarks of an intrinsically disordered protein,” International
Journal of Molecular Sciences, vol. 23, no. 11. MDPI, p. 6352, 2022.
ista: Bilanovičová V, Rýdza N, Koczka L, Hess M, Feraru E, Friml J, Nodzyński T.
2022. The hydrophilic loop of Arabidopsis PIN1 auxin efflux carrier harbors hallmarks
of an intrinsically disordered protein. International Journal of Molecular Sciences.
23(11), 6352.
mla: Bilanovičová, V., et al. “The Hydrophilic Loop of Arabidopsis PIN1 Auxin Efflux
Carrier Harbors Hallmarks of an Intrinsically Disordered Protein.” International
Journal of Molecular Sciences, vol. 23, no. 11, MDPI, 2022, p. 6352, doi:10.3390/ijms23116352.
short: V. Bilanovičová, N. Rýdza, L. Koczka, M. Hess, E. Feraru, J. Friml, T. Nodzyński,
International Journal of Molecular Sciences 23 (2022) 6352.
date_created: 2022-07-05T15:14:34Z
date_published: 2022-06-06T00:00:00Z
date_updated: 2023-08-09T10:13:57Z
day: '06'
ddc:
- '570'
department:
- _id: JiFr
doi: 10.3390/ijms23116352
external_id:
isi:
- '000808733300001'
pmid:
- '35683031'
file:
- access_level: open_access
checksum: e997a57a928ec9d51fad8ce824a05935
content_type: application/pdf
creator: cchlebak
date_created: 2022-07-06T07:36:59Z
date_updated: 2022-07-06T07:36:59Z
file_id: '11492'
file_name: 2022_IntJMolSci_Bilanovicova.pdf
file_size: 2324542
relation: main_file
success: 1
file_date_updated: 2022-07-06T07:36:59Z
has_accepted_license: '1'
intvolume: ' 23'
isi: 1
issue: '11'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
page: '6352'
pmid: 1
project:
- _id: 262EF96E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29988
name: RNA-directed DNA methylation in plant development
publication: International Journal of Molecular Sciences
publication_identifier:
issn:
- 1422-0067
publication_status: published
publisher: MDPI
quality_controlled: '1'
status: public
title: The hydrophilic loop of Arabidopsis PIN1 auxin efflux carrier harbors hallmarks
of an intrinsically disordered protein
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 23
year: '2022'
...
---
_id: '12144'
abstract:
- lang: eng
text: The phytohormone auxin is the major coordinative signal in plant development1,
mediating transcriptional reprogramming by a well-established canonical signalling
pathway. TRANSPORT INHIBITOR RESPONSE 1 (TIR1)/AUXIN-SIGNALING F-BOX (AFB) auxin
receptors are F-box subunits of ubiquitin ligase complexes. In response to auxin,
they associate with Aux/IAA transcriptional repressors and target them for degradation
via ubiquitination2,3. Here we identify adenylate cyclase (AC) activity as an
additional function of TIR1/AFB receptors across land plants. Auxin, together
with Aux/IAAs, stimulates cAMP production. Three separate mutations in the AC
motif of the TIR1 C-terminal region, all of which abolish the AC activity, each
render TIR1 ineffective in mediating gravitropism and sustained auxin-induced
root growth inhibition, and also affect auxin-induced transcriptional regulation.
These results highlight the importance of TIR1/AFB AC activity in canonical auxin
signalling. They also identify a unique phytohormone receptor cassette combining
F-box and AC motifs, and the role of cAMP as a second messenger in plants.
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
acknowledgement: This research was supported by the Lab Support Facility (LSF) and
the Imaging and Optics Facility (IOF) of IST Austria. We thank C. Gehring for suggestions
and advice; and K. U. Torii and G. Stacey for seeds and plasmids. This project was
funded by a European Research Council Advanced Grant (ETAP-742985). M.F.K. and R.N.
acknowledge the support of the EU MSCA-IF project CrysPINs (792329). M.K. was supported
by the project POWR.03.05.00-00-Z302/17 Universitas Copernicana Thoruniensis in
Futuro–IDS “Academia Copernicana”. CIDG acknowledges support from UKRI under Future
Leaders Fellowship grant number MR/T020652/1.
article_processing_charge: No
article_type: original
author:
- first_name: Linlin
full_name: Qi, Linlin
id: 44B04502-A9ED-11E9-B6FC-583AE6697425
last_name: Qi
orcid: 0000-0001-5187-8401
- first_name: Mateusz
full_name: Kwiatkowski, Mateusz
last_name: Kwiatkowski
- first_name: Huihuang
full_name: Chen, Huihuang
id: 83c96512-15b2-11ec-abd3-b7eede36184f
last_name: Chen
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
orcid: 0000-0001-8295-2926
- first_name: Scott A
full_name: Sinclair, Scott A
id: 2D99FE6A-F248-11E8-B48F-1D18A9856A87
last_name: Sinclair
orcid: 0000-0002-4566-0593
- first_name: Minxia
full_name: Zou, Minxia
id: 5c243f41-03f3-11ec-841c-96faf48a7ef9
last_name: Zou
- first_name: Charo I.
full_name: del Genio, Charo I.
last_name: del Genio
- first_name: Martin F.
full_name: Kubeš, Martin F.
last_name: Kubeš
- first_name: Richard
full_name: Napier, Richard
last_name: Napier
- first_name: Krzysztof
full_name: Jaworski, Krzysztof
last_name: Jaworski
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Qi L, Kwiatkowski M, Chen H, et al. Adenylate cyclase activity of TIR1/AFB
auxin receptors in plants. Nature. 2022;611(7934):133-138. doi:10.1038/s41586-022-05369-7
apa: Qi, L., Kwiatkowski, M., Chen, H., Hörmayer, L., Sinclair, S. A., Zou, M.,
… Friml, J. (2022). Adenylate cyclase activity of TIR1/AFB auxin receptors in
plants. Nature. Springer Nature. https://doi.org/10.1038/s41586-022-05369-7
chicago: Qi, Linlin, Mateusz Kwiatkowski, Huihuang Chen, Lukas Hörmayer, Scott A
Sinclair, Minxia Zou, Charo I. del Genio, et al. “Adenylate Cyclase Activity of
TIR1/AFB Auxin Receptors in Plants.” Nature. Springer Nature, 2022. https://doi.org/10.1038/s41586-022-05369-7.
ieee: L. Qi et al., “Adenylate cyclase activity of TIR1/AFB auxin receptors
in plants,” Nature, vol. 611, no. 7934. Springer Nature, pp. 133–138, 2022.
ista: Qi L, Kwiatkowski M, Chen H, Hörmayer L, Sinclair SA, Zou M, del Genio CI,
Kubeš MF, Napier R, Jaworski K, Friml J. 2022. Adenylate cyclase activity of TIR1/AFB
auxin receptors in plants. Nature. 611(7934), 133–138.
mla: Qi, Linlin, et al. “Adenylate Cyclase Activity of TIR1/AFB Auxin Receptors
in Plants.” Nature, vol. 611, no. 7934, Springer Nature, 2022, pp. 133–38,
doi:10.1038/s41586-022-05369-7.
short: L. Qi, M. Kwiatkowski, H. Chen, L. Hörmayer, S.A. Sinclair, M. Zou, C.I.
del Genio, M.F. Kubeš, R. Napier, K. Jaworski, J. Friml, Nature 611 (2022) 133–138.
date_created: 2023-01-12T12:06:05Z
date_published: 2022-11-03T00:00:00Z
date_updated: 2023-10-03T11:04:53Z
day: '03'
department:
- _id: JiFr
doi: 10.1038/s41586-022-05369-7
ec_funded: 1
external_id:
isi:
- '000875061600013'
pmid:
- '36289340'
intvolume: ' 611'
isi: 1
issue: '7934'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://wrap.warwick.ac.uk/168325/1/WRAP-denylate-cyclase-activity-TIR1-AFB-auxin-receptors-root-growth-22.pdf
month: '11'
oa: 1
oa_version: Submitted Version
page: 133-138
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Adenylate cyclase activity of TIR1/AFB auxin receptors in plants
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 611
year: '2022'
...
---
_id: '12120'
abstract:
- lang: eng
text: Plant root architecture flexibly adapts to changing nitrate (NO3−) availability
in the soil; however, the underlying molecular mechanism of this adaptive development
remains under-studied. To explore the regulation of NO3−-mediated root growth,
we screened for low-nitrate-resistant mutant (lonr) and identified mutants that
were defective in the NAC transcription factor NAC075 (lonr1) as being less sensitive
to low NO3− in terms of primary root growth. We show that NAC075 is a mobile transcription
factor relocating from the root stele tissues to the endodermis based on NO3−
availability. Under low-NO3− availability, the kinase CBL-interacting protein
kinase 1 (CIPK1) is activated, and it phosphorylates NAC075, restricting its movement
from the stele, which leads to the transcriptional regulation of downstream target
WRKY53, consequently leading to adapted root architecture. Our work thus identifies
an adaptive mechanism involving translocation of transcription factor based on
nutrient availability and leading to cell-specific reprogramming of plant root
growth.
acknowledgement: The authors are grateful to Jörg Kudla, Ying Miao, Yu Zheng, Gang
Li, and Jun Zheng for providing published materials and to Wenkun Zhou and Caifu
Jiang for helpful discussions. This work was supported by grants from the National
Key Research and Development Program of China (2021YFF1000500), the National Natural
Science Foundation of China (32170265 and 32022007), the Beijing Municipal Natural
Science Foundation (5192011), and the Chinese Universities Scientific Fund (2022TC153).
article_processing_charge: No
article_type: original
author:
- first_name: Huixin
full_name: Xiao, Huixin
last_name: Xiao
- first_name: Yumei
full_name: Hu, Yumei
last_name: Hu
- first_name: Yaping
full_name: Wang, Yaping
last_name: Wang
- first_name: Jinkui
full_name: Cheng, Jinkui
last_name: Cheng
- first_name: Jinyi
full_name: Wang, Jinyi
last_name: Wang
- first_name: Guojingwei
full_name: Chen, Guojingwei
last_name: Chen
- first_name: Qian
full_name: Li, Qian
last_name: Li
- first_name: Shuwei
full_name: Wang, Shuwei
last_name: Wang
- first_name: Yalu
full_name: Wang, Yalu
last_name: Wang
- first_name: Shao-Shuai
full_name: Wang, Shao-Shuai
last_name: Wang
- first_name: Yi
full_name: Wang, Yi
last_name: Wang
- first_name: Wei
full_name: Xuan, Wei
last_name: Xuan
- first_name: Zhen
full_name: Li, Zhen
last_name: Li
- first_name: Yan
full_name: Guo, Yan
last_name: Guo
- first_name: Zhizhong
full_name: Gong, Zhizhong
last_name: Gong
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Jing
full_name: Zhang, Jing
last_name: Zhang
citation:
ama: Xiao H, Hu Y, Wang Y, et al. Nitrate availability controls translocation of
the transcription factor NAC075 for cell-type-specific reprogramming of root growth.
Developmental Cell. 2022;57(23):2638-2651.e6. doi:10.1016/j.devcel.2022.11.006
apa: Xiao, H., Hu, Y., Wang, Y., Cheng, J., Wang, J., Chen, G., … Zhang, J. (2022).
Nitrate availability controls translocation of the transcription factor NAC075
for cell-type-specific reprogramming of root growth. Developmental Cell.
Elsevier. https://doi.org/10.1016/j.devcel.2022.11.006
chicago: Xiao, Huixin, Yumei Hu, Yaping Wang, Jinkui Cheng, Jinyi Wang, Guojingwei
Chen, Qian Li, et al. “Nitrate Availability Controls Translocation of the Transcription
Factor NAC075 for Cell-Type-Specific Reprogramming of Root Growth.” Developmental
Cell. Elsevier, 2022. https://doi.org/10.1016/j.devcel.2022.11.006.
ieee: H. Xiao et al., “Nitrate availability controls translocation of the
transcription factor NAC075 for cell-type-specific reprogramming of root growth,”
Developmental Cell, vol. 57, no. 23. Elsevier, p. 2638–2651.e6, 2022.
ista: Xiao H, Hu Y, Wang Y, Cheng J, Wang J, Chen G, Li Q, Wang S, Wang Y, Wang
S-S, Wang Y, Xuan W, Li Z, Guo Y, Gong Z, Friml J, Zhang J. 2022. Nitrate availability
controls translocation of the transcription factor NAC075 for cell-type-specific
reprogramming of root growth. Developmental Cell. 57(23), 2638–2651.e6.
mla: Xiao, Huixin, et al. “Nitrate Availability Controls Translocation of the Transcription
Factor NAC075 for Cell-Type-Specific Reprogramming of Root Growth.” Developmental
Cell, vol. 57, no. 23, Elsevier, 2022, p. 2638–2651.e6, doi:10.1016/j.devcel.2022.11.006.
short: H. Xiao, Y. Hu, Y. Wang, J. Cheng, J. Wang, G. Chen, Q. Li, S. Wang, Y. Wang,
S.-S. Wang, Y. Wang, W. Xuan, Z. Li, Y. Guo, Z. Gong, J. Friml, J. Zhang, Developmental
Cell 57 (2022) 2638–2651.e6.
date_created: 2023-01-12T11:57:00Z
date_published: 2022-12-05T00:00:00Z
date_updated: 2023-10-04T08:23:20Z
day: '05'
department:
- _id: JiFr
doi: 10.1016/j.devcel.2022.11.006
external_id:
isi:
- '000919603800005'
pmid:
- '36473460'
intvolume: ' 57'
isi: 1
issue: '23'
keyword:
- Developmental Biology
- Cell Biology
- General Biochemistry
- Genetics and Molecular Biology
- Molecular Biology
language:
- iso: eng
month: '12'
oa_version: None
page: 2638-2651.e6
pmid: 1
publication: Developmental Cell
publication_identifier:
issn:
- 1534-5807
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Nitrate availability controls translocation of the transcription factor NAC075
for cell-type-specific reprogramming of root growth
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 57
year: '2022'
...
---
_id: '12291'
abstract:
- lang: eng
text: The phytohormone auxin triggers transcriptional reprogramming through a well-characterized
perception machinery in the nucleus. By contrast, mechanisms that underlie fast
effects of auxin, such as the regulation of ion fluxes, rapid phosphorylation
of proteins or auxin feedback on its transport, remain unclear1,2,3. Whether auxin-binding
protein 1 (ABP1) is an auxin receptor has been a source of debate for decades1,4.
Here we show that a fraction of Arabidopsis thaliana ABP1 is secreted and binds
auxin specifically at an acidic pH that is typical of the apoplast. ABP1 and its
plasma-membrane-localized partner, transmembrane kinase 1 (TMK1), are required
for the auxin-induced ultrafast global phospho-response and for downstream processes
that include the activation of H+-ATPase and accelerated cytoplasmic streaming.
abp1 and tmk mutants cannot establish auxin-transporting channels and show defective
auxin-induced vasculature formation and regeneration. An ABP1(M2X) variant that
lacks the capacity to bind auxin is unable to complement these defects in abp1
mutants. These data indicate that ABP1 is the auxin receptor for TMK1-based cell-surface
signalling, which mediates the global phospho-response and auxin canalization.
acknowledged_ssus:
- _id: Bio
- _id: EM-Fac
- _id: LifeSc
acknowledgement: We acknowledge K. Kubiasová for excellent technical assistance, J.
Neuhold, A. Lehner and A. Sedivy for technical assistance with protein production
and purification at Vienna Biocenter Core Facilities; Creoptix for performing GCI;
and the Bioimaging, Electron Microscopy and Life Science Facilities at ISTA, the
Plant Sciences Core Facility of CEITEC Masaryk University, the Core Facility CELLIM
(MEYS CR, LM2018129 Czech-BioImaging) and J. Sprakel for their assistance. J.F.
is grateful to R. Napier for many insightful suggestions and support. We thank all
past and present members of the Friml group for their support and for other contributions
to this effort to clarify the controversial role of ABP1 over the past seven years.
The project received funding from the European Research Council (ERC) under the
European Union’s Horizon 2020 research and innovation program (grant agreement no.
742985 to J.F. and 833867 to D.W.); the Austrian Science Fund (FWF; P29988 to J.F.);
the Netherlands Organization for Scientific Research (NWO; VICI grant 865.14.001
to D.W. and VENI grant VI.Veni.212.003 to A.K.); the Ministry of Education, Science
and Technological Development of the Republic of Serbia (contract no. 451-03-68/2022-14/200053
to B.D.Ž.); and the MEXT/JSPS KAKENHI to K.T. (20K06685) and T.K. (20H05687 and
20H05910).
article_processing_charge: No
article_type: original
author:
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Zuzana
full_name: Gelová, Zuzana
id: 0AE74790-0E0B-11E9-ABC7-1ACFE5697425
last_name: Gelová
orcid: 0000-0003-4783-1752
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Ewa
full_name: Mazur, Ewa
last_name: Mazur
- first_name: Aline
full_name: Monzer, Aline
id: 2DB5D88C-D7B3-11E9-B8FD-7907E6697425
last_name: Monzer
- first_name: Lesia
full_name: Rodriguez Solovey, Lesia
id: 3922B506-F248-11E8-B48F-1D18A9856A87
last_name: Rodriguez Solovey
orcid: 0000-0002-7244-7237
- first_name: Mark
full_name: Roosjen, Mark
last_name: Roosjen
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Branka D.
full_name: Živanović, Branka D.
last_name: Živanović
- first_name: Minxia
full_name: Zou, Minxia
id: 5c243f41-03f3-11ec-841c-96faf48a7ef9
last_name: Zou
- first_name: Lukas
full_name: Fiedler, Lukas
id: 7c417475-8972-11ed-ae7b-8b674ca26986
last_name: Fiedler
- first_name: Caterina
full_name: Giannini, Caterina
id: e3fdddd5-f6e0-11ea-865d-ca99ee6367f4
last_name: Giannini
- first_name: Peter
full_name: Grones, Peter
last_name: Grones
- first_name: Mónika
full_name: Hrtyan, Mónika
id: 45A71A74-F248-11E8-B48F-1D18A9856A87
last_name: Hrtyan
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Andre
full_name: Kuhn, Andre
last_name: Kuhn
- first_name: Madhumitha
full_name: Narasimhan, Madhumitha
id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
last_name: Narasimhan
orcid: 0000-0002-8600-0671
- first_name: Marek
full_name: Randuch, Marek
id: 6ac4636d-15b2-11ec-abd3-fb8df79972ae
last_name: Randuch
- first_name: Nikola
full_name: Rýdza, Nikola
last_name: Rýdza
- first_name: Koji
full_name: Takahashi, Koji
last_name: Takahashi
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Anastasiia
full_name: Teplova, Anastasiia
id: e3736151-106c-11ec-b916-c2558e2762c6
last_name: Teplova
- first_name: Toshinori
full_name: Kinoshita, Toshinori
last_name: Kinoshita
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
- first_name: Hana
full_name: Rakusová, Hana
last_name: Rakusová
citation:
ama: Friml J, Gallei MC, Gelová Z, et al. ABP1–TMK auxin perception for global phosphorylation
and auxin canalization. Nature. 2022;609(7927):575-581. doi:10.1038/s41586-022-05187-x
apa: Friml, J., Gallei, M. C., Gelová, Z., Johnson, A. J., Mazur, E., Monzer, A.,
… Rakusová, H. (2022). ABP1–TMK auxin perception for global phosphorylation and
auxin canalization. Nature. Springer Nature. https://doi.org/10.1038/s41586-022-05187-x
chicago: Friml, Jiří, Michelle C Gallei, Zuzana Gelová, Alexander J Johnson, Ewa
Mazur, Aline Monzer, Lesia Rodriguez Solovey, et al. “ABP1–TMK Auxin Perception
for Global Phosphorylation and Auxin Canalization.” Nature. Springer Nature,
2022. https://doi.org/10.1038/s41586-022-05187-x.
ieee: J. Friml et al., “ABP1–TMK auxin perception for global phosphorylation
and auxin canalization,” Nature, vol. 609, no. 7927. Springer Nature, pp.
575–581, 2022.
ista: Friml J, Gallei MC, Gelová Z, Johnson AJ, Mazur E, Monzer A, Rodriguez Solovey
L, Roosjen M, Verstraeten I, Živanović BD, Zou M, Fiedler L, Giannini C, Grones
P, Hrtyan M, Kaufmann W, Kuhn A, Narasimhan M, Randuch M, Rýdza N, Takahashi K,
Tan S, Teplova A, Kinoshita T, Weijers D, Rakusová H. 2022. ABP1–TMK auxin perception
for global phosphorylation and auxin canalization. Nature. 609(7927), 575–581.
mla: Friml, Jiří, et al. “ABP1–TMK Auxin Perception for Global Phosphorylation and
Auxin Canalization.” Nature, vol. 609, no. 7927, Springer Nature, 2022,
pp. 575–81, doi:10.1038/s41586-022-05187-x.
short: J. Friml, M.C. Gallei, Z. Gelová, A.J. Johnson, E. Mazur, A. Monzer, L. Rodriguez
Solovey, M. Roosjen, I. Verstraeten, B.D. Živanović, M. Zou, L. Fiedler, C. Giannini,
P. Grones, M. Hrtyan, W. Kaufmann, A. Kuhn, M. Narasimhan, M. Randuch, N. Rýdza,
K. Takahashi, S. Tan, A. Teplova, T. Kinoshita, D. Weijers, H. Rakusová, Nature
609 (2022) 575–581.
date_created: 2023-01-16T10:04:48Z
date_published: 2022-09-15T00:00:00Z
date_updated: 2023-11-07T08:16:09Z
day: '15'
ddc:
- '580'
department:
- _id: JiFr
- _id: GradSch
- _id: EvBe
- _id: EM-Fac
doi: 10.1038/s41586-022-05187-x
ec_funded: 1
external_id:
isi:
- '000851357500002'
pmid:
- '36071161'
file:
- access_level: open_access
checksum: a6055c606aefb900bf62ae3e7d15f921
content_type: application/pdf
creator: amally
date_created: 2023-11-02T17:12:37Z
date_updated: 2023-11-02T17:12:37Z
file_id: '14483'
file_name: Friml Nature 2022_merged.pdf
file_size: 79774945
relation: main_file
success: 1
file_date_updated: 2023-11-02T17:12:37Z
has_accepted_license: '1'
intvolume: ' 609'
isi: 1
issue: '7927'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Submitted Version
page: 575-581
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 262EF96E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29988
name: RNA-directed DNA methylation in plant development
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: ABP1–TMK auxin perception for global phosphorylation and auxin canalization
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 609
year: '2022'
...
---
_id: '11626'
abstract:
- lang: eng
text: Plant growth and development is well known to be both, flexible and dynamic.
The high capacity for post-embryonic organ formation and tissue regeneration requires
tightly regulated intercellular communication and coordinated tissue polarization.
One of the most important drivers for patterning and polarity in plant development
is the phytohormone auxin. Auxin has the unique characteristic to establish polarized
channels for its own active directional cell to cell transport. This fascinating
phenomenon is called auxin canalization. Those auxin transport channels are characterized
by the expression and polar, subcellular localization of PIN auxin efflux carriers.
PIN proteins have the ability to dynamically change their localization and auxin
itself can affect this by interfering with trafficking. Most of the underlying
molecular mechanisms of canalization still remain enigmatic. What is known so
far is that canonical auxin signaling is indispensable but also other non-canonical
signaling components are thought to play a role. In order to shed light into the
mysteries auf auxin canalization this study revisits the branches of auxin signaling
in detail. Further a new auxin analogue, PISA, is developed which triggers auxin-like
responses but does not directly activate canonical transcriptional auxin signaling.
We revisit the direct auxin effect on PIN trafficking where we found that, contradictory
to previous observations, auxin is very specifically promoting endocytosis of
PIN2 but has no overall effect on endocytosis. Further, we evaluate which cellular
processes related to PIN subcellular dynamics are involved in the establishment
of auxin conducting channels and the formation of vascular tissue. We are re-evaluating
the function of AUXIN BINDING PROTEIN 1 (ABP1) and provide a comprehensive picture
about its developmental phneotypes and involvement in auxin signaling and canalization.
Lastly, we are focusing on the crosstalk between the hormone strigolactone (SL)
and auxin and found that SL is interfering with essentially all processes involved
in auxin canalization in a non-transcriptional manner. Lastly we identify a new
way of SL perception and signaling which is emanating from mitochondria, is independent
of canonical SL signaling and is modulating primary root growth.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
citation:
ama: Gallei MC. Auxin and strigolactone non-canonical signaling regulating development
in Arabidopsis thaliana. 2022. doi:10.15479/at:ista:11626
apa: Gallei, M. C. (2022). Auxin and strigolactone non-canonical signaling regulating
development in Arabidopsis thaliana. Institute of Science and Technology Austria.
https://doi.org/10.15479/at:ista:11626
chicago: Gallei, Michelle C. “Auxin and Strigolactone Non-Canonical Signaling Regulating
Development in Arabidopsis Thaliana.” Institute of Science and Technology Austria,
2022. https://doi.org/10.15479/at:ista:11626.
ieee: M. C. Gallei, “Auxin and strigolactone non-canonical signaling regulating
development in Arabidopsis thaliana,” Institute of Science and Technology Austria,
2022.
ista: Gallei MC. 2022. Auxin and strigolactone non-canonical signaling regulating
development in Arabidopsis thaliana. Institute of Science and Technology Austria.
mla: Gallei, Michelle C. Auxin and Strigolactone Non-Canonical Signaling Regulating
Development in Arabidopsis Thaliana. Institute of Science and Technology Austria,
2022, doi:10.15479/at:ista:11626.
short: M.C. Gallei, Auxin and Strigolactone Non-Canonical Signaling Regulating Development
in Arabidopsis Thaliana, Institute of Science and Technology Austria, 2022.
date_created: 2022-07-20T11:21:53Z
date_published: 2022-07-20T00:00:00Z
date_updated: 2023-11-07T08:20:13Z
day: '20'
ddc:
- '575'
degree_awarded: PhD
department:
- _id: GradSch
- _id: JiFr
doi: 10.15479/at:ista:11626
ec_funded: 1
file:
- access_level: open_access
checksum: bd7ac35403cf5b4b2607287d2a104b3a
content_type: application/pdf
creator: mgallei
date_created: 2022-07-25T09:08:47Z
date_updated: 2022-07-25T09:08:47Z
file_id: '11645'
file_name: Thesis_Gallei.pdf
file_size: 9730864
relation: main_file
- access_level: closed
checksum: a9e54fe5471ba25dc13c2150c1b8ccbb
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: mgallei
date_created: 2022-07-25T09:09:09Z
date_updated: 2022-07-25T09:39:58Z
file_id: '11646'
file_name: Thesis_Gallei_source.docx
file_size: 19560720
relation: source_file
- access_level: closed
checksum: 3994f7f20058941b5bb8a16886b21e71
content_type: application/pdf
creator: mgallei
date_created: 2022-07-25T09:09:32Z
date_updated: 2022-07-25T09:39:58Z
description: This is the print version of the thesis including the full appendix
file_id: '11647'
file_name: Thesis_Gallei_to_print.pdf
file_size: 24542837
relation: source_file
- access_level: open_access
checksum: f24acd3c0d864f4c6676e8b0d7bfa76b
content_type: application/pdf
creator: mgallei
date_created: 2022-07-25T11:48:45Z
date_updated: 2022-07-25T11:48:45Z
file_id: '11650'
file_name: Thesis_Gallei_Appendix.pdf
file_size: 15435966
relation: main_file
file_date_updated: 2022-07-25T11:48:45Z
has_accepted_license: '1'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
page: '248'
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication_identifier:
isbn:
- 978-3-99078-019-0
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '8931'
relation: part_of_dissertation
status: public
- id: '9287'
relation: part_of_dissertation
status: public
- id: '7142'
relation: part_of_dissertation
status: public
- id: '7465'
relation: part_of_dissertation
status: public
- id: '8138'
relation: part_of_dissertation
status: public
- id: '6260'
relation: part_of_dissertation
status: public
- id: '10411'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Eilon
full_name: Shani, Eilon
last_name: Shani
title: Auxin and strigolactone non-canonical signaling regulating development in Arabidopsis
thaliana
type: dissertation
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
year: '2022'
...
---
_id: '10411'
abstract:
- lang: eng
text: The phytohormone auxin is the major growth regulator governing tropic responses
including gravitropism. Auxin build-up at the lower side of stimulated shoots
promotes cell expansion, whereas in roots it inhibits growth, leading to upward
shoot bending and downward root bending, respectively. Yet it remains an enigma
how the same signal can trigger such opposite cellular responses. In this review,
we discuss several recent unexpected insights into the mechanisms underlying auxin
regulation of growth, challenging several existing models. We focus on the divergent
mechanisms of apoplastic pH regulation in shoots and roots revisiting the classical
Acid Growth Theory and discuss coordinated involvement of multiple auxin signaling
pathways. From this emerges a more comprehensive, updated picture how auxin regulates
growth.
acknowledgement: The authors thank Alexandra Mally for editing the text. This work
was supported by the Austrian Science Fund (FWF) I 3630-B25 to Jiří Friml and the
DOC Fellowship of the Austrian Academy of Sciences to Lanxin Li. All figures were
created with BioRender.com.
article_processing_charge: No
article_type: original
author:
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Li L, Gallei MC, Friml J. Bending to auxin: Fast acid growth for tropisms.
Trends in Plant Science. 2022;27(5):440-449. doi:10.1016/j.tplants.2021.11.006'
apa: 'Li, L., Gallei, M. C., & Friml, J. (2022). Bending to auxin: Fast acid
growth for tropisms. Trends in Plant Science. Cell Press. https://doi.org/10.1016/j.tplants.2021.11.006'
chicago: 'Li, Lanxin, Michelle C Gallei, and Jiří Friml. “Bending to Auxin: Fast
Acid Growth for Tropisms.” Trends in Plant Science. Cell Press, 2022. https://doi.org/10.1016/j.tplants.2021.11.006.'
ieee: 'L. Li, M. C. Gallei, and J. Friml, “Bending to auxin: Fast acid growth for
tropisms,” Trends in Plant Science, vol. 27, no. 5. Cell Press, pp. 440–449,
2022.'
ista: 'Li L, Gallei MC, Friml J. 2022. Bending to auxin: Fast acid growth for tropisms.
Trends in Plant Science. 27(5), 440–449.'
mla: 'Li, Lanxin, et al. “Bending to Auxin: Fast Acid Growth for Tropisms.” Trends
in Plant Science, vol. 27, no. 5, Cell Press, 2022, pp. 440–49, doi:10.1016/j.tplants.2021.11.006.'
short: L. Li, M.C. Gallei, J. Friml, Trends in Plant Science 27 (2022) 440–449.
date_created: 2021-12-05T23:01:43Z
date_published: 2022-05-01T00:00:00Z
date_updated: 2023-11-07T08:20:14Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.tplants.2021.11.006
external_id:
isi:
- '000793707900005'
pmid:
- '34848141'
file:
- access_level: open_access
checksum: 3d94980ee1ff6bec100dd813f6a921a6
content_type: application/pdf
creator: amally
date_created: 2023-11-02T17:00:03Z
date_updated: 2023-11-02T17:00:03Z
file_id: '14480'
file_name: Li Plants 2021_accepted.pdf
file_size: 805779
relation: main_file
success: 1
file_date_updated: 2023-11-02T17:00:03Z
has_accepted_license: '1'
intvolume: ' 27'
isi: 1
issue: '5'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Submitted Version
page: 440-449
pmid: 1
project:
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 26B4D67E-B435-11E9-9278-68D0E5697425
grant_number: '25351'
name: 'A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated
Rapid Growth Inhibition in Arabidopsis Root'
publication: Trends in Plant Science
publication_identifier:
issn:
- 1360-1385
publication_status: published
publisher: Cell Press
quality_controlled: '1'
related_material:
record:
- id: '11626'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: 'Bending to auxin: Fast acid growth for tropisms'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 27
year: '2022'
...
---
_id: '13240'
abstract:
- lang: eng
text: Ustilago maydis is a biotrophic phytopathogenic fungus that causes corn smut
disease. As a well-established model system, U. maydis is genetically fully accessible
with large omics datasets available and subject to various biological questions
ranging from DNA-repair, RNA-transport, and protein secretion to disease biology.
For many genetic approaches, tight control of transgene regulation is important.
Here we established an optimised version of the Tetracycline-ON (TetON) system
for U. maydis. We demonstrate the Tetracycline concentration-dependent expression
of fluorescent protein transgenes and the system’s suitability for the induced
expression of the toxic protein BCL2 Associated X-1 (Bax1). The Golden Gate compatible
vector system contains a native minimal promoter from the mating factor a-1 encoding
gene, mfa with ten copies of the tet-regulated operator (tetO) and a codon optimised
Tet-repressor (tetR*) which is translationally fused to the native transcriptional
corepressor Mql1 (UMAG_05501). The metabolism-independent transcriptional regulator
system is functional both, in liquid culture as well as on solid media in the
presence of the inducer and can become a useful tool for toxin-antitoxin studies,
identification of antifungal proteins, and to study functions of toxic gene products
in Ustilago maydis.
acknowledgement: "The research leading to these results received funding from the
European Research Council under the European Union’s Seventh Framework Programme
ERC-2013-STG (grant agreement: 335691), the Austrian Science Fund (I 3033-B22),
the Austrian Academy of Sciences, and the Deutsche Forschungsgemeinschaft (DFG,
German Research Foundation) under Germany's Excellence Strategy EXC-2070-390732324
(PhenoRob) and DFG grant (DJ 64/5-1).\r\nWe would like to thank the GMI/IMBA/IMP
core facilities for their excellent technical support. We would like to acknowledge
Dr. Sinéad A. O’Sullivan from DZNE, University of Bonn for providing anti-GFP antibodies.
The authors are thankful to the Excellence University of Bonn for providing infrastructure
and instrumentation facilities at the INRES-Plant Pathology department."
article_number: '1029114'
article_processing_charge: Yes
article_type: original
author:
- first_name: Kishor D.
full_name: Ingole, Kishor D.
last_name: Ingole
- first_name: Nithya
full_name: Nagarajan, Nithya
last_name: Nagarajan
- first_name: Simon
full_name: Uhse, Simon
last_name: Uhse
- first_name: Caterina
full_name: Giannini, Caterina
id: e3fdddd5-f6e0-11ea-865d-ca99ee6367f4
last_name: Giannini
- first_name: Armin
full_name: Djamei, Armin
last_name: Djamei
citation:
ama: Ingole KD, Nagarajan N, Uhse S, Giannini C, Djamei A. Tetracycline-controlled
(TetON) gene expression system for the smut fungus Ustilago maydis. Frontiers
in Fungal Biology. 2022;3. doi:10.3389/ffunb.2022.1029114
apa: Ingole, K. D., Nagarajan, N., Uhse, S., Giannini, C., & Djamei, A. (2022).
Tetracycline-controlled (TetON) gene expression system for the smut fungus Ustilago
maydis. Frontiers in Fungal Biology. Frontiers Media. https://doi.org/10.3389/ffunb.2022.1029114
chicago: Ingole, Kishor D., Nithya Nagarajan, Simon Uhse, Caterina Giannini, and
Armin Djamei. “Tetracycline-Controlled (TetON) Gene Expression System for the
Smut Fungus Ustilago Maydis.” Frontiers in Fungal Biology. Frontiers Media,
2022. https://doi.org/10.3389/ffunb.2022.1029114.
ieee: K. D. Ingole, N. Nagarajan, S. Uhse, C. Giannini, and A. Djamei, “Tetracycline-controlled
(TetON) gene expression system for the smut fungus Ustilago maydis,” Frontiers
in Fungal Biology, vol. 3. Frontiers Media, 2022.
ista: Ingole KD, Nagarajan N, Uhse S, Giannini C, Djamei A. 2022. Tetracycline-controlled
(TetON) gene expression system for the smut fungus Ustilago maydis. Frontiers
in Fungal Biology. 3, 1029114.
mla: Ingole, Kishor D., et al. “Tetracycline-Controlled (TetON) Gene Expression
System for the Smut Fungus Ustilago Maydis.” Frontiers in Fungal Biology,
vol. 3, 1029114, Frontiers Media, 2022, doi:10.3389/ffunb.2022.1029114.
short: K.D. Ingole, N. Nagarajan, S. Uhse, C. Giannini, A. Djamei, Frontiers in
Fungal Biology 3 (2022).
date_created: 2023-07-16T22:01:12Z
date_published: 2022-10-19T00:00:00Z
date_updated: 2024-03-06T14:01:57Z
day: '19'
ddc:
- '579'
department:
- _id: JiFr
doi: 10.3389/ffunb.2022.1029114
file:
- access_level: open_access
checksum: 2254e0119c0749d6f7237084fefcece6
content_type: application/pdf
creator: dernst
date_created: 2023-07-17T11:46:34Z
date_updated: 2023-07-17T11:46:34Z
file_id: '13242'
file_name: 2023_FrontiersFungalBio_Ingole.pdf
file_size: 27966699
relation: main_file
success: 1
file_date_updated: 2023-07-17T11:46:34Z
has_accepted_license: '1'
intvolume: ' 3'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
publication: Frontiers in Fungal Biology
publication_identifier:
eissn:
- 2673-6128
publication_status: published
publisher: Frontiers Media
quality_controlled: '1'
scopus_import: '1'
status: public
title: Tetracycline-controlled (TetON) gene expression system for the smut fungus
Ustilago maydis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 3
year: '2022'
...
---
_id: '10267'
abstract:
- lang: eng
text: Tropisms are among the most important growth responses for plant adaptation
to the surrounding environment. One of the most common tropisms is root gravitropism.
Root gravitropism enables the plant to anchor securely to the soil enabling the
absorption of water and nutrients. Most of the knowledge related to the plant
gravitropism has been acquired from the flowering plants, due to limited research
in non-seed plants. Limited research on non-seed plants is due in large part to
the lack of standard research methods. Here, we describe the experimental methods
to evaluate gravitropism in representative non-seed plant species, including the
non-vascular plant moss Physcomitrium patens, the early diverging extant vascular
plant lycophyte Selaginella moellendorffii and fern Ceratopteris richardii. In
addition, we introduce the methods used for statistical analysis of the root gravitropism
in non-seed plant species.
acknowledgement: The Ceratopteris richardii spores were obtained from the lab of Jo
Ann Banks at Purdue University. This work was supported by funding from the European
Union’s Horizon 2020 research and innovation program (ERC grant agreement number
742985), Austrian Science Fund (FWF, grant number I 3630-B25), IST Fellow program
and DOC Fellowship of the Austrian Academy of Sciences.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Zhang Y, Li L, Friml J. Evaluation of gravitropism in non-seed plants. In:
Blancaflor EB, ed. Plant Gravitropism. Vol 2368. MIMB. Springer Nature;
2021:43-51. doi:10.1007/978-1-0716-1677-2_2'
apa: Zhang, Y., Li, L., & Friml, J. (2021). Evaluation of gravitropism in non-seed
plants. In E. B. Blancaflor (Ed.), Plant Gravitropism (Vol. 2368, pp. 43–51).
Springer Nature. https://doi.org/10.1007/978-1-0716-1677-2_2
chicago: Zhang, Yuzhou, Lanxin Li, and Jiří Friml. “Evaluation of Gravitropism in
Non-Seed Plants.” In Plant Gravitropism, edited by Elison B Blancaflor,
2368:43–51. MIMB. Springer Nature, 2021. https://doi.org/10.1007/978-1-0716-1677-2_2.
ieee: Y. Zhang, L. Li, and J. Friml, “Evaluation of gravitropism in non-seed plants,”
in Plant Gravitropism, vol. 2368, E. B. Blancaflor, Ed. Springer Nature,
2021, pp. 43–51.
ista: 'Zhang Y, Li L, Friml J. 2021.Evaluation of gravitropism in non-seed plants.
In: Plant Gravitropism. Methods in Molecular Biology, vol. 2368, 43–51.'
mla: Zhang, Yuzhou, et al. “Evaluation of Gravitropism in Non-Seed Plants.” Plant
Gravitropism, edited by Elison B Blancaflor, vol. 2368, Springer Nature, 2021,
pp. 43–51, doi:10.1007/978-1-0716-1677-2_2.
short: Y. Zhang, L. Li, J. Friml, in:, E.B. Blancaflor (Ed.), Plant Gravitropism,
Springer Nature, 2021, pp. 43–51.
date_created: 2021-11-11T09:26:10Z
date_published: 2021-10-14T00:00:00Z
date_updated: 2022-08-26T09:13:00Z
day: '14'
department:
- _id: JiFr
doi: 10.1007/978-1-0716-1677-2_2
ec_funded: 1
editor:
- first_name: Elison B
full_name: Blancaflor, Elison B
last_name: Blancaflor
external_id:
pmid:
- '34647246'
intvolume: ' 2368'
language:
- iso: eng
month: '10'
oa_version: None
page: 43-51
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: Plant Gravitropism
publication_identifier:
eisbn:
- 978-1-0716-1677-2
isbn:
- 978-1-0716-1676-5
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
series_title: MIMB
status: public
title: Evaluation of gravitropism in non-seed plants
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2368
year: '2021'
...
---
_id: '10268'
abstract:
- lang: eng
text: The analysis of dynamic cellular processes such as plant cytokinesis stands
and falls with live-cell time-lapse confocal imaging. Conventional approaches
to time-lapse imaging of cell division in Arabidopsis root tips are tedious and
have low throughput. Here, we describe a protocol for long-term time-lapse simultaneous
imaging of multiple root tips on a vertical-stage confocal microscope with automated
root tracking. We also provide modifications of the basic protocol to implement
this imaging method in the analysis of genetic, pharmacological or laser ablation
wounding-mediated experimental manipulations. Our method dramatically improves
the efficiency of cell division time-lapse imaging by increasing the throughput,
while reducing the person-hour requirements of such experiments.
acknowledged_ssus:
- _id: Bio
acknowledgement: We thank B. De Rybel for allowing M.G. to work on this manuscript
during a postdoc in his laboratory, and EMBO for supporting M.G. with a Long-Term
fellowship (ALTF 1005-2019) during this time. We acknowledge the service and support
by the Bioimaging Facility at IST Austria, and finally, we thank A. Mally for proofreading
and correcting the manuscript.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
citation:
ama: 'Hörmayer L, Friml J, Glanc M. Automated time-lapse imaging and manipulation
of cell divisions in Arabidopsis roots by vertical-stage confocal microscopy.
In: Plant Cell Division. Vol 2382. MIMB. Humana Press; 2021:105-114. doi:10.1007/978-1-0716-1744-1_6'
apa: Hörmayer, L., Friml, J., & Glanc, M. (2021). Automated time-lapse imaging
and manipulation of cell divisions in Arabidopsis roots by vertical-stage confocal
microscopy. In Plant Cell Division (Vol. 2382, pp. 105–114). Humana Press.
https://doi.org/10.1007/978-1-0716-1744-1_6
chicago: Hörmayer, Lukas, Jiří Friml, and Matous Glanc. “Automated Time-Lapse Imaging
and Manipulation of Cell Divisions in Arabidopsis Roots by Vertical-Stage Confocal
Microscopy.” In Plant Cell Division, 2382:105–14. MIMB. Humana Press, 2021.
https://doi.org/10.1007/978-1-0716-1744-1_6.
ieee: L. Hörmayer, J. Friml, and M. Glanc, “Automated time-lapse imaging and manipulation
of cell divisions in Arabidopsis roots by vertical-stage confocal microscopy,”
in Plant Cell Division, vol. 2382, Humana Press, 2021, pp. 105–114.
ista: 'Hörmayer L, Friml J, Glanc M. 2021.Automated time-lapse imaging and manipulation
of cell divisions in Arabidopsis roots by vertical-stage confocal microscopy.
In: Plant Cell Division. Methods in Molecular Biology, vol. 2382, 105–114.'
mla: Hörmayer, Lukas, et al. “Automated Time-Lapse Imaging and Manipulation of Cell
Divisions in Arabidopsis Roots by Vertical-Stage Confocal Microscopy.” Plant
Cell Division, vol. 2382, Humana Press, 2021, pp. 105–14, doi:10.1007/978-1-0716-1744-1_6.
short: L. Hörmayer, J. Friml, M. Glanc, in:, Plant Cell Division, Humana Press,
2021, pp. 105–114.
date_created: 2021-11-11T10:03:30Z
date_published: 2021-10-28T00:00:00Z
date_updated: 2022-06-03T06:47:06Z
day: '28'
department:
- _id: JiFr
doi: 10.1007/978-1-0716-1744-1_6
external_id:
pmid:
- '34705235'
intvolume: ' 2382'
language:
- iso: eng
month: '10'
oa_version: None
page: 105-114
pmid: 1
publication: Plant Cell Division
publication_identifier:
eisbn:
- 978-1-0716-1744-1
eissn:
- 1940-6029
isbn:
- 978-1-0716-1743-4
issn:
- 1064-3745
publication_status: published
publisher: Humana Press
quality_controlled: '1'
scopus_import: '1'
series_title: MIMB
status: public
title: Automated time-lapse imaging and manipulation of cell divisions in Arabidopsis
roots by vertical-stage confocal microscopy
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2382
year: '2021'
...
---
_id: '8582'
abstract:
- lang: eng
text: "Cell and tissue polarization is fundamental for plant growth and morphogenesis.
The polar, cellular localization of Arabidopsis PIN‐FORMED (PIN) proteins is crucial
for their function in directional auxin transport. The clustering of PIN polar
cargoes within the plasma membrane has been proposed to be important for the maintenance
of their polar distribution. However, the more detailed features of PIN clusters
and the cellular requirements of cargo clustering remain unclear.\r\nHere, we
characterized PIN clusters in detail by means of multiple advanced microscopy
and quantification methods, such as 3D quantitative imaging or freeze‐fracture
replica labeling. The size and aggregation types of PIN clusters were determined
by electron microscopy at the nanometer level at different polar domains and at
different developmental stages, revealing a strong preference for clustering at
the polar domains.\r\nPharmacological and genetic studies revealed that PIN clusters
depend on phosphoinositol pathways, cytoskeletal structures and specific cell‐wall
components as well as connections between the cell wall and the plasma membrane.\r\nThis
study identifies the role of different cellular processes and structures in polar
cargo clustering and provides initial mechanistic insight into the maintenance
of polarity in plants and other systems."
acknowledged_ssus:
- _id: Bio
acknowledgement: We thank Dr Ingo Heilmann (Martin‐Luther‐University Halle‐Wittenberg)
for the XVE>>PIP5K1‐YFP line, Dr Brad Day (Michigan State University) for the ndr1‐1
mutant and the complementation lines, and Dr Patricia C. Zambryski (University of
California, Berkeley) for the 35S::P30‐GFP line, the Bioimaging team (IST Austria)
for assistance with imaging, group members for discussions, Martine De Cock for
help in preparing the manuscript and Nataliia Gnyliukh for critical reading and
revision of the manuscript. This project received funding from the European Research
Council (ERC) under the European Union's Horizon 2020 research and innovation program
(grant agreement No. 742985) and Comisión Nacional de Investigación Científica y
Tecnológica (Project CONICYT‐PAI 82130047). DvW received funding from the People
Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme
(FP7/2007‐2013) under REA grant agreement no. 291734.
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Hongjiang
full_name: Li, Hongjiang
id: 33CA54A6-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0001-5039-9660
- first_name: Daniel
full_name: von Wangenheim, Daniel
id: 49E91952-F248-11E8-B48F-1D18A9856A87
last_name: von Wangenheim
orcid: 0000-0002-6862-1247
- first_name: Xixi
full_name: Zhang, Xixi
id: 61A66458-47E9-11EA-85BA-8AEAAF14E49A
last_name: Zhang
orcid: 0000-0001-7048-4627
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Nasser
full_name: Darwish-Miranda, Nasser
id: 39CD9926-F248-11E8-B48F-1D18A9856A87
last_name: Darwish-Miranda
orcid: 0000-0002-8821-8236
- first_name: Satoshi
full_name: Naramoto, Satoshi
last_name: Naramoto
- first_name: Krzysztof T
full_name: Wabnik, Krzysztof T
id: 4DE369A4-F248-11E8-B48F-1D18A9856A87
last_name: Wabnik
orcid: 0000-0001-7263-0560
- first_name: Riet
full_name: de Rycke, Riet
last_name: de Rycke
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Daniel J
full_name: Gütl, Daniel J
id: 381929CE-F248-11E8-B48F-1D18A9856A87
last_name: Gütl
- first_name: Ricardo
full_name: Tejos, Ricardo
last_name: Tejos
- first_name: Peter
full_name: Grones, Peter
id: 399876EC-F248-11E8-B48F-1D18A9856A87
last_name: Grones
- first_name: Meiyu
full_name: Ke, Meiyu
last_name: Ke
- first_name: Xu
full_name: Chen, Xu
id: 4E5ADCAA-F248-11E8-B48F-1D18A9856A87
last_name: Chen
- first_name: Jan
full_name: Dettmer, Jan
last_name: Dettmer
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Li H, von Wangenheim D, Zhang X, et al. Cellular requirements for PIN polar
cargo clustering in Arabidopsis thaliana. New Phytologist. 2021;229(1):351-369.
doi:10.1111/nph.16887
apa: Li, H., von Wangenheim, D., Zhang, X., Tan, S., Darwish-Miranda, N., Naramoto,
S., … Friml, J. (2021). Cellular requirements for PIN polar cargo clustering in
Arabidopsis thaliana. New Phytologist. Wiley. https://doi.org/10.1111/nph.16887
chicago: Li, Hongjiang, Daniel von Wangenheim, Xixi Zhang, Shutang Tan, Nasser Darwish-Miranda,
Satoshi Naramoto, Krzysztof T Wabnik, et al. “Cellular Requirements for PIN Polar
Cargo Clustering in Arabidopsis Thaliana.” New Phytologist. Wiley, 2021.
https://doi.org/10.1111/nph.16887.
ieee: H. Li et al., “Cellular requirements for PIN polar cargo clustering
in Arabidopsis thaliana,” New Phytologist, vol. 229, no. 1. Wiley, pp.
351–369, 2021.
ista: Li H, von Wangenheim D, Zhang X, Tan S, Darwish-Miranda N, Naramoto S, Wabnik
KT, de Rycke R, Kaufmann W, Gütl DJ, Tejos R, Grones P, Ke M, Chen X, Dettmer
J, Friml J. 2021. Cellular requirements for PIN polar cargo clustering in Arabidopsis
thaliana. New Phytologist. 229(1), 351–369.
mla: Li, Hongjiang, et al. “Cellular Requirements for PIN Polar Cargo Clustering
in Arabidopsis Thaliana.” New Phytologist, vol. 229, no. 1, Wiley, 2021,
pp. 351–69, doi:10.1111/nph.16887.
short: H. Li, D. von Wangenheim, X. Zhang, S. Tan, N. Darwish-Miranda, S. Naramoto,
K.T. Wabnik, R. de Rycke, W. Kaufmann, D.J. Gütl, R. Tejos, P. Grones, M. Ke,
X. Chen, J. Dettmer, J. Friml, New Phytologist 229 (2021) 351–369.
date_created: 2020-09-28T08:59:28Z
date_published: 2021-01-01T00:00:00Z
date_updated: 2023-08-04T11:01:21Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
- _id: EM-Fac
- _id: Bio
- _id: EvBe
doi: 10.1111/nph.16887
ec_funded: 1
external_id:
isi:
- '000570187900001'
file:
- access_level: open_access
checksum: b45621607b4cab97eeb1605ab58e896e
content_type: application/pdf
creator: dernst
date_created: 2021-02-04T09:44:17Z
date_updated: 2021-02-04T09:44:17Z
file_id: '9084'
file_name: 2021_NewPhytologist_Li.pdf
file_size: 4061962
relation: main_file
success: 1
file_date_updated: 2021-02-04T09:44:17Z
has_accepted_license: '1'
intvolume: ' 229'
isi: 1
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: 351-369
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
publication: New Phytologist
publication_identifier:
eissn:
- '14698137'
issn:
- 0028646X
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 229
year: '2021'
...
---
_id: '8606'
abstract:
- lang: eng
text: The leaf is a crucial organ evolved with remarkable morphological diversity
to maximize plant photosynthesis. The leaf shape is a key trait that affects photosynthesis,
flowering rates, disease resistance, and yield. Although many genes regulating
leaf development have been identified in the past years, the precise regulatory
architecture underlying the generation of diverse leaf shapes remains to be elucidated.
We used cotton as a reference model to probe the genetic framework underlying
divergent leaf forms. Comparative transcriptome analysis revealed that the GhARF16‐1
and GhKNOX2‐1 genes might be potential regulators of leaf shape. We functionally
characterized the auxin‐responsive factor ARF16‐1 acting upstream of GhKNOX2‐1
to determine leaf morphology in cotton. The transcription of GhARF16‐1 was significantly
higher in lobed‐leaved cotton than in smooth‐leaved cotton. Furthermore, the overexpression
of GhARF16‐1 led to the upregulation of GhKNOX2‐1 and resulted in more and deeper
serrations in cotton leaves, similar to the leaf shape of cotton plants overexpressing
GhKNOX2‐1. We found that GhARF16‐1 specifically bound to the promoter of GhKNOX2‐1
to induce its expression. The heterologous expression of GhARF16‐1 and GhKNOX2‐1
in Arabidopsis led to lobed and curly leaves, and a genetic analysis revealed
that GhKNOX2‐1 is epistatic to GhARF16‐1 in Arabidopsis, suggesting that the GhARF16‐1
and GhKNOX2‐1 interaction paradigm also functions to regulate leaf shape in Arabidopsis.
To our knowledge, our results uncover a novel mechanism by which auxin, through
the key component ARF16‐1 and its downstream‐activated gene KNOX2‐1, determines
leaf morphology in eudicots.
acknowledgement: We are thankful to Professor Yuxian Zhu from Wuhan University for
his extremely valuable remarks and helpful comments on the manuscript. This work
was supported by the Shaanxi Natural Science Foundation (2019JQ‐062 and 2020JQ‐410),
Shaanxi Youth Entrusted Talents Program (20190205), China Postdoctoral Science Foundation
(2018M640947, 2020T130394), Shaanxi Postdoctoral Project (2018BSHYDZZ76), Natural
Science Basic Research Plan in Shaanxi Province of China (2018JZ3006), Fundamental
Research Funds for the Central Universities (GK201903064, GK201901004, GK202002005
and GK202001004), and State Key Laboratory of Cotton Biology Open Fund (CB2020A12).
article_processing_charge: No
article_type: original
author:
- first_name: P
full_name: He, P
last_name: He
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: H
full_name: Li, H
last_name: Li
- first_name: X
full_name: Fu, X
last_name: Fu
- first_name: H
full_name: Shang, H
last_name: Shang
- first_name: C
full_name: Zou, C
last_name: Zou
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: G
full_name: Xiao, G
last_name: Xiao
citation:
ama: He P, Zhang Y, Li H, et al. GhARF16-1 modulates leaf development by transcriptionally
regulating the GhKNOX2-1 gene in cotton. Plant Biotechnology Journal. 2021;19(3):548-562.
doi:10.1111/pbi.13484
apa: He, P., Zhang, Y., Li, H., Fu, X., Shang, H., Zou, C., … Xiao, G. (2021). GhARF16-1
modulates leaf development by transcriptionally regulating the GhKNOX2-1 gene
in cotton. Plant Biotechnology Journal. Wiley. https://doi.org/10.1111/pbi.13484
chicago: He, P, Yuzhou Zhang, H Li, X Fu, H Shang, C Zou, Jiří Friml, and G Xiao.
“GhARF16-1 Modulates Leaf Development by Transcriptionally Regulating the GhKNOX2-1
Gene in Cotton.” Plant Biotechnology Journal. Wiley, 2021. https://doi.org/10.1111/pbi.13484.
ieee: P. He et al., “GhARF16-1 modulates leaf development by transcriptionally
regulating the GhKNOX2-1 gene in cotton,” Plant Biotechnology Journal,
vol. 19, no. 3. Wiley, pp. 548–562, 2021.
ista: He P, Zhang Y, Li H, Fu X, Shang H, Zou C, Friml J, Xiao G. 2021. GhARF16-1
modulates leaf development by transcriptionally regulating the GhKNOX2-1 gene
in cotton. Plant Biotechnology Journal. 19(3), 548–562.
mla: He, P., et al. “GhARF16-1 Modulates Leaf Development by Transcriptionally Regulating
the GhKNOX2-1 Gene in Cotton.” Plant Biotechnology Journal, vol. 19, no.
3, Wiley, 2021, pp. 548–62, doi:10.1111/pbi.13484.
short: P. He, Y. Zhang, H. Li, X. Fu, H. Shang, C. Zou, J. Friml, G. Xiao, Plant
Biotechnology Journal 19 (2021) 548–562.
date_created: 2020-10-05T12:44:33Z
date_published: 2021-03-01T00:00:00Z
date_updated: 2023-08-04T11:03:10Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/pbi.13484
external_id:
isi:
- '000577682300001'
pmid:
- '32981232'
file:
- access_level: open_access
checksum: 63845be37fb962586e0c7773f2355970
content_type: application/pdf
creator: dernst
date_created: 2021-04-12T12:29:07Z
date_updated: 2021-04-12T12:29:07Z
file_id: '9321'
file_name: 2021_PlantBiotechJournal_He.pdf
file_size: 15691871
relation: main_file
success: 1
file_date_updated: 2021-04-12T12:29:07Z
has_accepted_license: '1'
intvolume: ' 19'
isi: 1
issue: '3'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
page: 548-562
pmid: 1
publication: Plant Biotechnology Journal
publication_identifier:
issn:
- 1467-7644
- 1467-7652
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: GhARF16-1 modulates leaf development by transcriptionally regulating the GhKNOX2-1
gene in cotton
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 19
year: '2021'
...
---
_id: '8992'
abstract:
- lang: eng
text: The phytohormone auxin plays a central role in shaping plant growth and development.
With decades of genetic and biochemical studies, numerous core molecular components
and their networks, underlying auxin biosynthesis, transport, and signaling, have
been identified. Notably, protein phosphorylation, catalyzed by kinases and oppositely
hydrolyzed by phosphatases, has been emerging to be a crucial type of post-translational
modification, regulating physiological and developmental auxin output at all levels.
In this review, we comprehensively discuss earlier and recent advances in our
understanding of genetics, biochemistry, and cell biology of the kinases and phosphatases
participating in auxin action. We provide insights into the mechanisms by which
reversible protein phosphorylation defines developmental auxin responses, discuss
current challenges, and provide our perspectives on future directions involving
the integration of the control of protein phosphorylation into the molecular auxin
network.
acknowledgement: This work was supported by the European Union’s Horizon 2020 Program
(ERC grant agreement no. 742985 to J.F.). S.T. was funded by a European Molecular
Biology Organization (EMBO) long-term postdoctoral fellowship (ALTF 723-2015). C.L.
is supported by the Austrian Science Fund (FWF; P 31493).
article_processing_charge: No
article_type: original
author:
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Christian
full_name: Luschnig, Christian
last_name: Luschnig
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Tan S, Luschnig C, Friml J. Pho-view of auxin: Reversible protein phosphorylation
in auxin biosynthesis, transport and signaling. Molecular Plant. 2021;14(1):151-165.
doi:10.1016/j.molp.2020.11.004'
apa: 'Tan, S., Luschnig, C., & Friml, J. (2021). Pho-view of auxin: Reversible
protein phosphorylation in auxin biosynthesis, transport and signaling. Molecular
Plant. Elsevier. https://doi.org/10.1016/j.molp.2020.11.004'
chicago: 'Tan, Shutang, Christian Luschnig, and Jiří Friml. “Pho-View of Auxin:
Reversible Protein Phosphorylation in Auxin Biosynthesis, Transport and Signaling.”
Molecular Plant. Elsevier, 2021. https://doi.org/10.1016/j.molp.2020.11.004.'
ieee: 'S. Tan, C. Luschnig, and J. Friml, “Pho-view of auxin: Reversible protein
phosphorylation in auxin biosynthesis, transport and signaling,” Molecular
Plant, vol. 14, no. 1. Elsevier, pp. 151–165, 2021.'
ista: 'Tan S, Luschnig C, Friml J. 2021. Pho-view of auxin: Reversible protein phosphorylation
in auxin biosynthesis, transport and signaling. Molecular Plant. 14(1), 151–165.'
mla: 'Tan, Shutang, et al. “Pho-View of Auxin: Reversible Protein Phosphorylation
in Auxin Biosynthesis, Transport and Signaling.” Molecular Plant, vol.
14, no. 1, Elsevier, 2021, pp. 151–65, doi:10.1016/j.molp.2020.11.004.'
short: S. Tan, C. Luschnig, J. Friml, Molecular Plant 14 (2021) 151–165.
date_created: 2021-01-03T23:01:23Z
date_published: 2021-01-04T00:00:00Z
date_updated: 2023-08-04T11:21:13Z
day: '04'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.molp.2020.11.004
ec_funded: 1
external_id:
isi:
- '000605359400014'
pmid:
- '33186755'
file:
- access_level: open_access
checksum: 917e60e57092f22e16beac70b1775ea6
content_type: application/pdf
creator: dernst
date_created: 2021-01-07T14:03:53Z
date_updated: 2021-01-07T14:03:53Z
file_id: '8995'
file_name: 2020_MolecularPlant_Tan.pdf
file_size: 871088
relation: main_file
success: 1
file_date_updated: 2021-01-07T14:03:53Z
has_accepted_license: '1'
intvolume: ' 14'
isi: 1
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: 151-165
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 256FEF10-B435-11E9-9278-68D0E5697425
grant_number: 723-2015
name: Long Term Fellowship
publication: Molecular Plant
publication_identifier:
eissn:
- '17529867'
issn:
- '16742052'
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Pho-view of auxin: Reversible protein phosphorylation in auxin biosynthesis,
transport and signaling'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 14
year: '2021'
...
---
_id: '8993'
abstract:
- lang: eng
text: N-1-naphthylphthalamic acid (NPA) is a key inhibitor of directional (polar)
transport of the hormone auxin in plants. For decades, it has been a pivotal tool
in elucidating the unique polar auxin transport-based processes underlying plant
growth and development. Its exact mode of action has long been sought after and
is still being debated, with prevailing mechanistic schemes describing only indirect
connections between NPA and the main transporters responsible for directional
transport, namely PIN auxin exporters. Here we present data supporting a model
in which NPA associates with PINs in a more direct manner than hitherto postulated.
We show that NPA inhibits PIN activity in a heterologous oocyte system and that
expression of NPA-sensitive PINs in plant, yeast, and oocyte membranes leads to
specific saturable NPA binding. We thus propose that PINs are a bona fide NPA
target. This offers a straightforward molecular basis for NPA inhibition of PIN-dependent
auxin transport and a logical parsimonious explanation for the known physiological
effects of NPA on plant growth, as well as an alternative hypothesis to interpret
past and future results. We also introduce PIN dimerization and describe an effect
of NPA on this, suggesting that NPA binding could be exploited to gain insights
into structural aspects of PINs related to their transport mechanism.
acknowledgement: "This work was supported by Austrian Science Fund Grant FWF P21533-B20
(to L.A.); German Research Foundation Grant DFG HA3468/6-1 (to U.Z.H.); and European
Research Council Grant 742985 (to J.F.). We thank Herta Steinkellner and Alexandra
Castilho for N. benthamiana plants, Fabian Nagelreiter for statistical advice, Lanassa
Bassukas for help with [ɣ32P]-\r\nATP assays, and Josef Penninger for providing
access to mass spectrometry instruments at the Vienna BioCenter Core Facilities.
We thank PNAS reviewers for the many comments and suggestions that helped to improve
this manuscript."
article_number: e2020857118
article_processing_charge: No
article_type: original
author:
- first_name: Lindy
full_name: Abas, Lindy
last_name: Abas
- first_name: Martina
full_name: Kolb, Martina
last_name: Kolb
- first_name: Johannes
full_name: Stadlmann, Johannes
last_name: Stadlmann
- first_name: Dorina P.
full_name: Janacek, Dorina P.
last_name: Janacek
- first_name: Kristina
full_name: Lukic, Kristina
id: 2B04DB84-F248-11E8-B48F-1D18A9856A87
last_name: Lukic
orcid: 0000-0003-1581-881X
- first_name: Claus
full_name: Schwechheimer, Claus
last_name: Schwechheimer
- first_name: Leonid A
full_name: Sazanov, Leonid A
id: 338D39FE-F248-11E8-B48F-1D18A9856A87
last_name: Sazanov
orcid: 0000-0002-0977-7989
- first_name: Lukas
full_name: Mach, Lukas
last_name: Mach
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Ulrich Z.
full_name: Hammes, Ulrich Z.
last_name: Hammes
citation:
ama: Abas L, Kolb M, Stadlmann J, et al. Naphthylphthalamic acid associates with
and inhibits PIN auxin transporters. PNAS. 2021;118(1). doi:10.1073/pnas.2020857118
apa: Abas, L., Kolb, M., Stadlmann, J., Janacek, D. P., Lukic, K., Schwechheimer,
C., … Hammes, U. Z. (2021). Naphthylphthalamic acid associates with and inhibits
PIN auxin transporters. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.2020857118
chicago: Abas, Lindy, Martina Kolb, Johannes Stadlmann, Dorina P. Janacek, Kristina
Lukic, Claus Schwechheimer, Leonid A Sazanov, Lukas Mach, Jiří Friml, and Ulrich
Z. Hammes. “Naphthylphthalamic Acid Associates with and Inhibits PIN Auxin Transporters.”
PNAS. National Academy of Sciences, 2021. https://doi.org/10.1073/pnas.2020857118.
ieee: L. Abas et al., “Naphthylphthalamic acid associates with and inhibits
PIN auxin transporters,” PNAS, vol. 118, no. 1. National Academy of Sciences,
2021.
ista: Abas L, Kolb M, Stadlmann J, Janacek DP, Lukic K, Schwechheimer C, Sazanov
LA, Mach L, Friml J, Hammes UZ. 2021. Naphthylphthalamic acid associates with
and inhibits PIN auxin transporters. PNAS. 118(1), e2020857118.
mla: Abas, Lindy, et al. “Naphthylphthalamic Acid Associates with and Inhibits PIN
Auxin Transporters.” PNAS, vol. 118, no. 1, e2020857118, National Academy
of Sciences, 2021, doi:10.1073/pnas.2020857118.
short: L. Abas, M. Kolb, J. Stadlmann, D.P. Janacek, K. Lukic, C. Schwechheimer,
L.A. Sazanov, L. Mach, J. Friml, U.Z. Hammes, PNAS 118 (2021).
date_created: 2021-01-03T23:01:23Z
date_published: 2021-01-05T00:00:00Z
date_updated: 2023-08-07T13:29:23Z
day: '05'
department:
- _id: JiFr
- _id: LeSa
doi: 10.1073/pnas.2020857118
ec_funded: 1
external_id:
isi:
- '000607270100073'
pmid:
- '33443187'
intvolume: ' 118'
isi: 1
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1073/pnas.2020857118
month: '01'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: PNAS
publication_identifier:
eissn:
- '10916490'
issn:
- '00278424'
publication_status: published
publisher: National Academy of Sciences
quality_controlled: '1'
related_material:
link:
- relation: erratum
url: https://doi.org/10.1073/pnas.2102232118
scopus_import: '1'
status: public
title: Naphthylphthalamic acid associates with and inhibits PIN auxin transporters
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 118
year: '2021'
...
---
_id: '9254'
abstract:
- lang: eng
text: 'Auxin is a key regulator of plant growth and development. Local auxin biosynthesis
and intercellular transport generates regional gradients in the root that are
instructive for processes such as specification of developmental zones that maintain
root growth and tropic responses. Here we present a toolbox to study auxin-mediated
root development that features: (i) the ability to control auxin synthesis with
high spatio-temporal resolution and (ii) single-cell nucleus tracking and morphokinetic
analysis infrastructure. Integration of these two features enables cutting-edge
analysis of root development at single-cell resolution based on morphokinetic
parameters under normal growth conditions and during cell-type-specific induction
of auxin biosynthesis. We show directional auxin flow in the root and refine the
contributions of key players in this process. In addition, we determine the quantitative
kinetics of Arabidopsis root meristem skewing, which depends on local auxin gradients
but does not require PIN2 and AUX1 auxin transporter activities. Beyond the mechanistic
insights into root development, the tools developed here will enable biologists
to study kinetics and morphology of various critical processes at the single cell-level
in whole organisms.'
acknowledgement: This work was supported by grants from the Israel Science Foundation
(2378/19 to E.S.), the Joint NSFC-ISF Research Grant (3419/20 to E.S. and Z.D.),
the Human Frontier Science Program (HFSP—LIY000540/2020 to E.S.), the European Research
Council Starting Grant (757683- RobustHormoneTrans to E.S.), PBC postdoctoral fellowships
(to Y.H. and M.O.), NIH (GM114660 to Y.Z.), Breast Cancer Research Foundation (BCRF
to I.T.).
article_number: '1657'
article_processing_charge: No
article_type: original
author:
- first_name: Yangjie
full_name: Hu, Yangjie
last_name: Hu
- first_name: Moutasem
full_name: Omary, Moutasem
last_name: Omary
- first_name: Yun
full_name: Hu, Yun
last_name: Hu
- first_name: Ohad
full_name: Doron, Ohad
last_name: Doron
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
- first_name: Qingguo
full_name: Chen, Qingguo
last_name: Chen
- first_name: Or
full_name: Megides, Or
last_name: Megides
- first_name: Ori
full_name: Chekli, Ori
last_name: Chekli
- first_name: Zhaojun
full_name: Ding, Zhaojun
last_name: Ding
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Yunde
full_name: Zhao, Yunde
last_name: Zhao
- first_name: Ilan
full_name: Tsarfaty, Ilan
last_name: Tsarfaty
- first_name: Eilon
full_name: Shani, Eilon
last_name: Shani
citation:
ama: Hu Y, Omary M, Hu Y, et al. Cell kinetics of auxin transport and activity in
Arabidopsis root growth and skewing. Nature Communications. 2021;12. doi:10.1038/s41467-021-21802-3
apa: Hu, Y., Omary, M., Hu, Y., Doron, O., Hörmayer, L., Chen, Q., … Shani, E. (2021).
Cell kinetics of auxin transport and activity in Arabidopsis root growth and skewing.
Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-021-21802-3
chicago: Hu, Yangjie, Moutasem Omary, Yun Hu, Ohad Doron, Lukas Hörmayer, Qingguo
Chen, Or Megides, et al. “Cell Kinetics of Auxin Transport and Activity in Arabidopsis
Root Growth and Skewing.” Nature Communications. Springer Nature, 2021.
https://doi.org/10.1038/s41467-021-21802-3.
ieee: Y. Hu et al., “Cell kinetics of auxin transport and activity in Arabidopsis
root growth and skewing,” Nature Communications, vol. 12. Springer Nature,
2021.
ista: Hu Y, Omary M, Hu Y, Doron O, Hörmayer L, Chen Q, Megides O, Chekli O, Ding
Z, Friml J, Zhao Y, Tsarfaty I, Shani E. 2021. Cell kinetics of auxin transport
and activity in Arabidopsis root growth and skewing. Nature Communications. 12,
1657.
mla: Hu, Yangjie, et al. “Cell Kinetics of Auxin Transport and Activity in Arabidopsis
Root Growth and Skewing.” Nature Communications, vol. 12, 1657, Springer
Nature, 2021, doi:10.1038/s41467-021-21802-3.
short: Y. Hu, M. Omary, Y. Hu, O. Doron, L. Hörmayer, Q. Chen, O. Megides, O. Chekli,
Z. Ding, J. Friml, Y. Zhao, I. Tsarfaty, E. Shani, Nature Communications 12 (2021).
date_created: 2021-03-21T23:01:19Z
date_published: 2021-03-12T00:00:00Z
date_updated: 2023-08-07T14:17:55Z
day: '12'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41467-021-21802-3
external_id:
isi:
- '000630419400048'
pmid:
- '33712581'
file:
- access_level: open_access
checksum: e1022f3aee349853ded2b2b3e092362d
content_type: application/pdf
creator: dernst
date_created: 2021-03-22T11:18:58Z
date_updated: 2021-03-22T11:18:58Z
file_id: '9275'
file_name: 2021_NatureComm_Hu.pdf
file_size: 8602096
relation: main_file
success: 1
file_date_updated: 2021-03-22T11:18:58Z
has_accepted_license: '1'
intvolume: ' 12'
isi: 1
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
publication: Nature Communications
publication_identifier:
eissn:
- '20411723'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Cell kinetics of auxin transport and activity in Arabidopsis root growth and
skewing
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 12
year: '2021'
...
---
_id: '9443'
abstract:
- lang: eng
text: Endoplasmic reticulum–plasma membrane contact sites (ER–PM CS) play fundamental
roles in all eukaryotic cells. Arabidopsis thaliana mutants lacking the ER–PM
protein tether synaptotagmin1 (SYT1) exhibit decreased PM integrity under multiple
abiotic stresses, such as freezing, high salt, osmotic stress, and mechanical
damage. Here, we show that, together with SYT1, the stress-induced SYT3 is an
ER–PM tether that also functions in maintaining PM integrity. The ER–PM CS localization
of SYT1 and SYT3 is dependent on PM phosphatidylinositol-4-phosphate and is regulated
by abiotic stress. Lipidomic analysis revealed that cold stress increased the
accumulation of diacylglycerol at the PM in a syt1/3 double mutant relative to
wild-type while the levels of most glycerolipid species remain unchanged. In addition,
the SYT1-green fluorescent protein fusion preferentially binds diacylglycerol
in vivo with little affinity for polar glycerolipids. Our work uncovers a SYT-dependent
mechanism of stress adaptation counteracting the detrimental accumulation of diacylglycerol
at the PM produced during episodes of abiotic stress.
acknowledgement: "We would also like to thank Lothar Willmitzer for the lipidomic
analysis at the Max Planck Institute of Molecular Plant Physiology (Potsdam, Germany).
We thank Manuela Vega from SCI for her technical assistance in image analysis. We
thank John R. Pearson and the Bionand Nanoimaging Unit, F. David Navas Fernández
and the SCAI Imaging Facility and The Plant Cell Biology facility at the Shanghai
Center for Plant Stress Biology for assistance with confocal microscopy. The FaFAH1
clone was a gift from Iraida Amaya Saavedra (IFAPA-Centro de Churriana, Málaga,
Spain). The AHA3 antibody against the H+-ATPase was a gift from Ramón Serrano Salom
(Instituto de Biología Molecular y Celular de Plantas, Valencia, Spain). The MAP-mTU2-SAC1
construct was provided by Yvon Jaillais (Laboratoire Reproduction et Développement
des Plantes, Univ Lyon, France). The pGWB5 from the pGWB vector series, was provided
by Tsuyoshi Nakagawa (Department of Molecular and Functional Genomics, Shimane University).
We thank Plan Propio from the University of Málaga for financial support.\r\nFunding"
article_processing_charge: No
article_type: original
author:
- first_name: N
full_name: Ruiz-Lopez, N
last_name: Ruiz-Lopez
- first_name: J
full_name: Pérez-Sancho, J
last_name: Pérez-Sancho
- first_name: A
full_name: Esteban Del Valle, A
last_name: Esteban Del Valle
- first_name: RP
full_name: Haslam, RP
last_name: Haslam
- first_name: S
full_name: Vanneste, S
last_name: Vanneste
- first_name: R
full_name: Catalá, R
last_name: Catalá
- first_name: C
full_name: Perea-Resa, C
last_name: Perea-Resa
- first_name: D
full_name: Van Damme, D
last_name: Van Damme
- first_name: S
full_name: García-Hernández, S
last_name: García-Hernández
- first_name: A
full_name: Albert, A
last_name: Albert
- first_name: J
full_name: Vallarino, J
last_name: Vallarino
- first_name: J
full_name: Lin, J
last_name: Lin
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: AP
full_name: Macho, AP
last_name: Macho
- first_name: J
full_name: Salinas, J
last_name: Salinas
- first_name: A
full_name: Rosado, A
last_name: Rosado
- first_name: JA
full_name: Napier, JA
last_name: Napier
- first_name: V
full_name: Amorim-Silva, V
last_name: Amorim-Silva
- first_name: MA
full_name: Botella, MA
last_name: Botella
citation:
ama: Ruiz-Lopez N, Pérez-Sancho J, Esteban Del Valle A, et al. Synaptotagmins at
the endoplasmic reticulum-plasma membrane contact sites maintain diacylglycerol
homeostasis during abiotic stress. Plant Cell. 2021;33(7):2431-2453. doi:10.1093/plcell/koab122
apa: Ruiz-Lopez, N., Pérez-Sancho, J., Esteban Del Valle, A., Haslam, R., Vanneste,
S., Catalá, R., … Botella, M. (2021). Synaptotagmins at the endoplasmic reticulum-plasma
membrane contact sites maintain diacylglycerol homeostasis during abiotic stress.
Plant Cell. American Society of Plant Biologists. https://doi.org/10.1093/plcell/koab122
chicago: Ruiz-Lopez, N, J Pérez-Sancho, A Esteban Del Valle, RP Haslam, S Vanneste,
R Catalá, C Perea-Resa, et al. “Synaptotagmins at the Endoplasmic Reticulum-Plasma
Membrane Contact Sites Maintain Diacylglycerol Homeostasis during Abiotic Stress.”
Plant Cell. American Society of Plant Biologists, 2021. https://doi.org/10.1093/plcell/koab122.
ieee: N. Ruiz-Lopez et al., “Synaptotagmins at the endoplasmic reticulum-plasma
membrane contact sites maintain diacylglycerol homeostasis during abiotic stress,”
Plant Cell, vol. 33, no. 7. American Society of Plant Biologists, pp. 2431–2453,
2021.
ista: Ruiz-Lopez N, Pérez-Sancho J, Esteban Del Valle A, Haslam R, Vanneste S, Catalá
R, Perea-Resa C, Van Damme D, García-Hernández S, Albert A, Vallarino J, Lin J,
Friml J, Macho A, Salinas J, Rosado A, Napier J, Amorim-Silva V, Botella M. 2021.
Synaptotagmins at the endoplasmic reticulum-plasma membrane contact sites maintain
diacylglycerol homeostasis during abiotic stress. Plant Cell. 33(7), 2431–2453.
mla: Ruiz-Lopez, N., et al. “Synaptotagmins at the Endoplasmic Reticulum-Plasma
Membrane Contact Sites Maintain Diacylglycerol Homeostasis during Abiotic Stress.”
Plant Cell, vol. 33, no. 7, American Society of Plant Biologists, 2021,
pp. 2431–53, doi:10.1093/plcell/koab122.
short: N. Ruiz-Lopez, J. Pérez-Sancho, A. Esteban Del Valle, R. Haslam, S. Vanneste,
R. Catalá, C. Perea-Resa, D. Van Damme, S. García-Hernández, A. Albert, J. Vallarino,
J. Lin, J. Friml, A. Macho, J. Salinas, A. Rosado, J. Napier, V. Amorim-Silva,
M. Botella, Plant Cell 33 (2021) 2431–2453.
date_created: 2021-06-02T13:13:58Z
date_published: 2021-07-01T00:00:00Z
date_updated: 2023-08-08T13:54:32Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1093/plcell/koab122
ec_funded: 1
external_id:
isi:
- '000703938100026'
pmid:
- '33944955'
file:
- access_level: open_access
checksum: 22d596678d00310d793611864a6d0fcd
content_type: application/pdf
creator: cchlebak
date_created: 2021-10-14T13:36:38Z
date_updated: 2021-10-14T13:36:38Z
file_id: '10141'
file_name: 2021_PlantCell_RuizLopez.pdf
file_size: 2952028
relation: main_file
success: 1
file_date_updated: 2021-10-14T13:36:38Z
has_accepted_license: '1'
intvolume: ' 33'
isi: 1
issue: '7'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
page: 2431-2453
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Plant Cell
publication_identifier:
eissn:
- 1532-298x
issn:
- 1040-4651
publication_status: published
publisher: American Society of Plant Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Synaptotagmins at the endoplasmic reticulum-plasma membrane contact sites maintain
diacylglycerol homeostasis during abiotic stress
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 33
year: '2021'
...
---
_id: '9657'
abstract:
- lang: eng
text: To overcome nitrogen deficiency, legume roots establish symbiotic interactions
with nitrogen-fixing rhizobia that is fostered in specialized organs (nodules).
Similar to other organs, nodule formation is determined by a local maximum of
the phytohormone auxin at the primordium site. However, how auxin regulates nodule
development remains poorly understood. Here, we found that in soybean, (Glycine
max), dynamic auxin transport driven by PIN-FORMED (PIN) transporter GmPIN1 is
involved in nodule primordium formation. GmPIN1 was specifically expressed in
nodule primordium cells and GmPIN1 was polarly localized in these cells. Two nodulation
regulators, (iso)flavonoids trigger expanded distribution of GmPIN1b to root cortical
cells, and cytokinin rearranges GmPIN1b polarity. Gmpin1abc triple mutants generated
with CRISPR-Cas9 showed impaired establishment of auxin maxima in nodule meristems
and aberrant divisions in the nodule primordium cells. Moreover, overexpression
of GmPIN1 suppressed nodule primordium initiation. GmPIN9d, an ortholog of Arabidopsis
thaliana PIN2, acts together with GmPIN1 later in nodule development to acropetally
transport auxin in vascular bundles, fine-tuning the auxin supply for nodule enlargement.
Our findings reveal how PIN-dependent auxin transport modulates different aspects
of soybean nodule development and suggest that establishment of auxin gradient
is a prerequisite for the proper interaction between legumes and rhizobia.
article_processing_charge: No
article_type: original
author:
- first_name: Z
full_name: Gao, Z
last_name: Gao
- first_name: Z
full_name: Chen, Z
last_name: Chen
- first_name: Y
full_name: Cui, Y
last_name: Cui
- first_name: M
full_name: Ke, M
last_name: Ke
- first_name: H
full_name: Xu, H
last_name: Xu
- first_name: Q
full_name: Xu, Q
last_name: Xu
- first_name: J
full_name: Chen, J
last_name: Chen
- first_name: Y
full_name: Li, Y
last_name: Li
- first_name: L
full_name: Huang, L
last_name: Huang
- first_name: H
full_name: Zhao, H
last_name: Zhao
- first_name: D
full_name: Huang, D
last_name: Huang
- first_name: S
full_name: Mai, S
last_name: Mai
- first_name: T
full_name: Xu, T
last_name: Xu
- first_name: X
full_name: Liu, X
last_name: Liu
- first_name: S
full_name: Li, S
last_name: Li
- first_name: Y
full_name: Guan, Y
last_name: Guan
- first_name: W
full_name: Yang, W
last_name: Yang
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: J
full_name: Petrášek, J
last_name: Petrášek
- first_name: J
full_name: Zhang, J
last_name: Zhang
- first_name: X
full_name: Chen, X
last_name: Chen
citation:
ama: Gao Z, Chen Z, Cui Y, et al. GmPIN-dependent polar auxin transport is involved
in soybean nodule development. Plant Cell. 2021;33(9):2981–3003. doi:10.1093/plcell/koab183
apa: Gao, Z., Chen, Z., Cui, Y., Ke, M., Xu, H., Xu, Q., … Chen, X. (2021). GmPIN-dependent
polar auxin transport is involved in soybean nodule development. Plant Cell.
American Society of Plant Biologists. https://doi.org/10.1093/plcell/koab183
chicago: Gao, Z, Z Chen, Y Cui, M Ke, H Xu, Q Xu, J Chen, et al. “GmPIN-Dependent
Polar Auxin Transport Is Involved in Soybean Nodule Development.” Plant Cell.
American Society of Plant Biologists, 2021. https://doi.org/10.1093/plcell/koab183.
ieee: Z. Gao et al., “GmPIN-dependent polar auxin transport is involved in
soybean nodule development,” Plant Cell, vol. 33, no. 9. American Society
of Plant Biologists, pp. 2981–3003, 2021.
ista: Gao Z, Chen Z, Cui Y, Ke M, Xu H, Xu Q, Chen J, Li Y, Huang L, Zhao H, Huang
D, Mai S, Xu T, Liu X, Li S, Guan Y, Yang W, Friml J, Petrášek J, Zhang J, Chen
X. 2021. GmPIN-dependent polar auxin transport is involved in soybean nodule development.
Plant Cell. 33(9), 2981–3003.
mla: Gao, Z., et al. “GmPIN-Dependent Polar Auxin Transport Is Involved in Soybean
Nodule Development.” Plant Cell, vol. 33, no. 9, American Society of Plant
Biologists, 2021, pp. 2981–3003, doi:10.1093/plcell/koab183.
short: Z. Gao, Z. Chen, Y. Cui, M. Ke, H. Xu, Q. Xu, J. Chen, Y. Li, L. Huang, H.
Zhao, D. Huang, S. Mai, T. Xu, X. Liu, S. Li, Y. Guan, W. Yang, J. Friml, J. Petrášek,
J. Zhang, X. Chen, Plant Cell 33 (2021) 2981–3003.
date_created: 2021-07-14T15:32:43Z
date_published: 2021-07-07T00:00:00Z
date_updated: 2023-08-10T14:01:41Z
day: '07'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1093/plcell/koab183
external_id:
isi:
- '000702165300012'
pmid:
- '34240197'
file:
- access_level: open_access
checksum: 6715712ec306c321f0204c817b7f8ae7
content_type: application/pdf
creator: cziletti
date_created: 2021-07-19T12:13:34Z
date_updated: 2021-07-19T12:13:34Z
file_id: '9691'
file_name: 2021_PlantCell_Gao.pdf
file_size: 10566921
relation: main_file
success: 1
file_date_updated: 2021-07-19T12:13:34Z
has_accepted_license: '1'
intvolume: ' 33'
isi: 1
issue: '9'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
page: 2981–3003
pmid: 1
publication: Plant Cell
publication_identifier:
eissn:
- 1532-298x
issn:
- 1040-4651
publication_status: published
publisher: American Society of Plant Biologists
quality_controlled: '1'
status: public
title: GmPIN-dependent polar auxin transport is involved in soybean nodule development
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 33
year: '2021'
...
---
_id: '9656'
abstract:
- lang: eng
text: Tropisms, growth responses to environmental stimuli such as light or gravity,
are spectacular examples of adaptive plant development. The plant hormone auxin
serves as a major coordinative signal. The PIN auxin exporters, through their
dynamic polar subcellular localizations, redirect auxin fluxes in response to
environmental stimuli and the resulting auxin gradients across organs underly
differential cell elongation and bending. In this review, we discuss recent advances
concerning regulations of PIN polarity during tropisms, focusing on PIN phosphorylation
and trafficking. We also cover how environmental cues regulate PIN actions during
tropisms, and a crucial role of auxin feedback on PIN polarity during bending
termination. Finally, the interactions between different tropisms are reviewed
to understand plant adaptive growth in the natural environment.
acknowledgement: We are grateful to Lukas Fiedler, Alexandra Mally (IST Austria) and
Dr. Bartel Vanholme (VIB, Ghent) for their critical comments on the manuscript.
We apologize to those researchers whose great work was not cited. This work is supported
by the European Research Council under the European Union’s Horizon 2020 research
and innovation Programme (ERC grant agreement number 742985), and the Austrian Science
Fund (FWF, grant number I 3630-B25) to JF. HH is supported by the China Scholarship
Council (CSC scholarship, 201506870018) and a starting grant from Jiangxi Agriculture
University (9232308314).
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Linlin
full_name: Qi, Linlin
id: 44B04502-A9ED-11E9-B6FC-583AE6697425
last_name: Qi
orcid: 0000-0001-5187-8401
- first_name: SS
full_name: Alotaibi, SS
last_name: Alotaibi
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Han H, Adamowski M, Qi L, Alotaibi S, Friml J. PIN-mediated polar auxin transport
regulations in plant tropic responses. New Phytologist. 2021;232(2):510-522.
doi:10.1111/nph.17617
apa: Han, H., Adamowski, M., Qi, L., Alotaibi, S., & Friml, J. (2021). PIN-mediated
polar auxin transport regulations in plant tropic responses. New Phytologist.
Wiley. https://doi.org/10.1111/nph.17617
chicago: Han, Huibin, Maciek Adamowski, Linlin Qi, SS Alotaibi, and Jiří Friml.
“PIN-Mediated Polar Auxin Transport Regulations in Plant Tropic Responses.” New
Phytologist. Wiley, 2021. https://doi.org/10.1111/nph.17617.
ieee: H. Han, M. Adamowski, L. Qi, S. Alotaibi, and J. Friml, “PIN-mediated polar
auxin transport regulations in plant tropic responses,” New Phytologist,
vol. 232, no. 2. Wiley, pp. 510–522, 2021.
ista: Han H, Adamowski M, Qi L, Alotaibi S, Friml J. 2021. PIN-mediated polar auxin
transport regulations in plant tropic responses. New Phytologist. 232(2), 510–522.
mla: Han, Huibin, et al. “PIN-Mediated Polar Auxin Transport Regulations in Plant
Tropic Responses.” New Phytologist, vol. 232, no. 2, Wiley, 2021, pp. 510–22,
doi:10.1111/nph.17617.
short: H. Han, M. Adamowski, L. Qi, S. Alotaibi, J. Friml, New Phytologist 232 (2021)
510–522.
date_created: 2021-07-14T15:29:14Z
date_published: 2021-10-01T00:00:00Z
date_updated: 2023-08-10T14:02:41Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/nph.17617
ec_funded: 1
external_id:
isi:
- '000680587100001'
pmid:
- '34254313'
file:
- access_level: open_access
checksum: 6422a6eb329b52d96279daaee0fcf189
content_type: application/pdf
creator: kschuh
date_created: 2021-10-07T13:42:47Z
date_updated: 2021-10-07T13:42:47Z
file_id: '10105'
file_name: 2021_NewPhytologist_Han.pdf
file_size: 1939800
relation: main_file
success: 1
file_date_updated: 2021-10-07T13:42:47Z
has_accepted_license: '1'
intvolume: ' 232'
isi: 1
issue: '2'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
page: 510-522
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: New Phytologist
publication_identifier:
eissn:
- 1469-8137
issn:
- 0028-646x
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: PIN-mediated polar auxin transport regulations in plant tropic responses
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 232
year: '2021'
...
---
_id: '9909'
abstract:
- lang: eng
text: Roots are composed of different root types and, in the dicotyledonous Arabidopsis,
typically consist of a primary root that branches into lateral roots. Adventitious
roots emerge from non-root tissue and are formed upon wounding or other types
of abiotic stress. Here, we investigated adventitious root (AR) formation in Arabidopsis
hypocotyls under conditions of altered abscisic acid (ABA) signaling. Exogenously
applied ABA suppressed AR formation at 0.25 µM or higher doses. AR formation was
less sensitive to the synthetic ABA analog pyrabactin (PB). However, PB was a
more potent inhibitor at concentrations above 1 µM, suggesting that it was more
selective in triggering a root inhibition response. Analysis of a series of phosphonamide
and phosphonate pyrabactin analogs suggested that adventitious root formation
and lateral root branching are differentially regulated by ABA signaling. ABA
biosynthesis and signaling mutants affirmed a general inhibitory role of ABA and
point to PYL1 and PYL2 as candidate ABA receptors that regulate AR inhibition.
acknowledgement: We thank S. Cutler (Riverside, USA) for providing the ABA biosynthesis
mutants and ABA signaling mutants.
article_number: '1141'
article_processing_charge: Yes
article_type: original
author:
- first_name: Yinwei
full_name: Zeng, Yinwei
last_name: Zeng
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Hoang Khai
full_name: Trinh, Hoang Khai
last_name: Trinh
- first_name: Thomas
full_name: Heugebaert, Thomas
last_name: Heugebaert
- first_name: Christian V.
full_name: Stevens, Christian V.
last_name: Stevens
- first_name: Irene
full_name: Garcia-Maquilon, Irene
last_name: Garcia-Maquilon
- first_name: Pedro L.
full_name: Rodriguez, Pedro L.
last_name: Rodriguez
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Danny
full_name: Geelen, Danny
last_name: Geelen
citation:
ama: Zeng Y, Verstraeten I, Trinh HK, et al. Arabidopsis hypocotyl adventitious
root formation is suppressed by ABA signaling. Genes. 2021;12(8). doi:10.3390/genes12081141
apa: Zeng, Y., Verstraeten, I., Trinh, H. K., Heugebaert, T., Stevens, C. V., Garcia-Maquilon,
I., … Geelen, D. (2021). Arabidopsis hypocotyl adventitious root formation is
suppressed by ABA signaling. Genes. MDPI. https://doi.org/10.3390/genes12081141
chicago: Zeng, Yinwei, Inge Verstraeten, Hoang Khai Trinh, Thomas Heugebaert, Christian
V. Stevens, Irene Garcia-Maquilon, Pedro L. Rodriguez, Steffen Vanneste, and Danny
Geelen. “Arabidopsis Hypocotyl Adventitious Root Formation Is Suppressed by ABA
Signaling.” Genes. MDPI, 2021. https://doi.org/10.3390/genes12081141.
ieee: Y. Zeng et al., “Arabidopsis hypocotyl adventitious root formation
is suppressed by ABA signaling,” Genes, vol. 12, no. 8. MDPI, 2021.
ista: Zeng Y, Verstraeten I, Trinh HK, Heugebaert T, Stevens CV, Garcia-Maquilon
I, Rodriguez PL, Vanneste S, Geelen D. 2021. Arabidopsis hypocotyl adventitious
root formation is suppressed by ABA signaling. Genes. 12(8), 1141.
mla: Zeng, Yinwei, et al. “Arabidopsis Hypocotyl Adventitious Root Formation Is
Suppressed by ABA Signaling.” Genes, vol. 12, no. 8, 1141, MDPI, 2021,
doi:10.3390/genes12081141.
short: Y. Zeng, I. Verstraeten, H.K. Trinh, T. Heugebaert, C.V. Stevens, I. Garcia-Maquilon,
P.L. Rodriguez, S. Vanneste, D. Geelen, Genes 12 (2021).
date_created: 2021-08-15T22:01:28Z
date_published: 2021-07-27T00:00:00Z
date_updated: 2023-08-11T10:32:21Z
day: '27'
ddc:
- '580'
- '570'
department:
- _id: JiFr
doi: 10.3390/genes12081141
external_id:
isi:
- '000690558000001'
file:
- access_level: open_access
checksum: 3d99535618cf9a5b14d264408fa52e97
content_type: application/pdf
creator: asandaue
date_created: 2021-08-16T09:02:40Z
date_updated: 2021-08-16T09:02:40Z
file_id: '9919'
file_name: 2021_Genes_Zeng.pdf
file_size: 1340305
relation: main_file
success: 1
file_date_updated: 2021-08-16T09:02:40Z
has_accepted_license: '1'
intvolume: ' 12'
isi: 1
issue: '8'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
publication: Genes
publication_identifier:
eissn:
- '20734425'
publication_status: published
publisher: MDPI
quality_controlled: '1'
scopus_import: '1'
status: public
title: Arabidopsis hypocotyl adventitious root formation is suppressed by ABA signaling
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 12
year: '2021'
...