---
_id: '11723'
abstract:
- lang: eng
text: Plant cell growth responds rapidly to various stimuli, adapting architecture
to environmental changes. Two major endogenous signals regulating growth are the
phytohormone auxin and the secreted peptides rapid alkalinization factors (RALFs).
Both trigger very rapid cellular responses and also exert long-term effects [Du
et al., Annu. Rev. Plant Biol. 71, 379–402 (2020); Blackburn et al., Plant Physiol.
182, 1657–1666 (2020)]. However, the way, in which these distinct signaling pathways
converge to regulate growth, remains unknown. Here, using vertical confocal microscopy
combined with a microfluidic chip, we addressed the mechanism of RALF action on
growth. We observed correlation between RALF1-induced rapid Arabidopsis thaliana
root growth inhibition and apoplast alkalinization during the initial phase of
the response, and revealed that RALF1 reversibly inhibits primary root growth
through apoplast alkalinization faster than within 1 min. This rapid apoplast
alkalinization was the result of RALF1-induced net H+ influx and was mediated
by the receptor FERONIA (FER). Furthermore, we investigated the cross-talk between
RALF1 and the auxin signaling pathways during root growth regulation. The results
showed that RALF-FER signaling triggered auxin signaling with a delay of approximately
1 h by up-regulating auxin biosynthesis, thus contributing to sustained RALF1-induced
growth inhibition. This biphasic RALF1 action on growth allows plants to respond
rapidly to environmental stimuli and also reprogram growth and development in
the long term.
acknowledgement: We thank Sarah M. Assmann, Kris Vissenberg, and Nadine Paris for
kindly sharing seeds; Matyáš Fendrych for initiating this project and providing
constant support; Lukas Fiedler for revising the manuscript; and Huibin Han and
Arseny Savin for contributing to genotyping. This work was supported by the Austrian
Science Fund (FWF) I 3630-B25 (to J.F.) and the Doctoral Fellowship Progrmme of
the Austrian Academy of Sciences (to L.L.) We also acknowledge Taif University Researchers
Supporting Project TURSP-HC2021/02 and funding “Plants as a tool for sustainable
global development (no. CZ.02.1.01/0.0/0.0/16_019/0000827).”
article_number: e2121058119
article_processing_charge: No
article_type: original
author:
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Huihuang
full_name: Chen, Huihuang
id: 83c96512-15b2-11ec-abd3-b7eede36184f
last_name: Chen
- first_name: Saqer S.
full_name: Alotaibi, Saqer S.
last_name: Alotaibi
- first_name: Aleš
full_name: Pěnčík, Aleš
last_name: Pěnčík
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Ondřej
full_name: Novák, Ondřej
last_name: Novák
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Li L, Chen H, Alotaibi SS, et al. RALF1 peptide triggers biphasic root growth
inhibition upstream of auxin biosynthesis. Proceedings of the National Academy
of Sciences. 2022;119(31). doi:10.1073/pnas.2121058119
apa: Li, L., Chen, H., Alotaibi, S. S., Pěnčík, A., Adamowski, M., Novák, O., &
Friml, J. (2022). RALF1 peptide triggers biphasic root growth inhibition upstream
of auxin biosynthesis. Proceedings of the National Academy of Sciences.
Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.2121058119
chicago: Li, Lanxin, Huihuang Chen, Saqer S. Alotaibi, Aleš Pěnčík, Maciek Adamowski,
Ondřej Novák, and Jiří Friml. “RALF1 Peptide Triggers Biphasic Root Growth Inhibition
Upstream of Auxin Biosynthesis.” Proceedings of the National Academy of Sciences.
Proceedings of the National Academy of Sciences, 2022. https://doi.org/10.1073/pnas.2121058119.
ieee: L. Li et al., “RALF1 peptide triggers biphasic root growth inhibition
upstream of auxin biosynthesis,” Proceedings of the National Academy of Sciences,
vol. 119, no. 31. Proceedings of the National Academy of Sciences, 2022.
ista: Li L, Chen H, Alotaibi SS, Pěnčík A, Adamowski M, Novák O, Friml J. 2022.
RALF1 peptide triggers biphasic root growth inhibition upstream of auxin biosynthesis.
Proceedings of the National Academy of Sciences. 119(31), e2121058119.
mla: Li, Lanxin, et al. “RALF1 Peptide Triggers Biphasic Root Growth Inhibition
Upstream of Auxin Biosynthesis.” Proceedings of the National Academy of Sciences,
vol. 119, no. 31, e2121058119, Proceedings of the National Academy of Sciences,
2022, doi:10.1073/pnas.2121058119.
short: L. Li, H. Chen, S.S. Alotaibi, A. Pěnčík, M. Adamowski, O. Novák, J. Friml,
Proceedings of the National Academy of Sciences 119 (2022).
date_created: 2022-08-04T20:06:49Z
date_published: 2022-07-25T00:00:00Z
date_updated: 2023-08-03T12:43:53Z
day: '25'
ddc:
- '580'
department:
- _id: GradSch
- _id: JiFr
doi: 10.1073/pnas.2121058119
external_id:
isi:
- '000881496900002'
pmid:
- '35878023'
file:
- access_level: open_access
checksum: ae6f19b0d9efba6687f9e4dc1bab1d6e
content_type: application/pdf
creator: dernst
date_created: 2022-08-08T07:42:09Z
date_updated: 2022-08-08T07:42:09Z
file_id: '11747'
file_name: 2022_PNAS_Li.pdf
file_size: 2506262
relation: main_file
success: 1
file_date_updated: 2022-08-08T07:42:09Z
has_accepted_license: '1'
intvolume: ' 119'
isi: 1
issue: '31'
keyword:
- Multidisciplinary
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '07'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 26B4D67E-B435-11E9-9278-68D0E5697425
grant_number: '25351'
name: 'A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated
Rapid Growth Inhibition in Arabidopsis Root'
publication: Proceedings of the National Academy of Sciences
publication_identifier:
eissn:
- 1091-6490
issn:
- 0027-8424
publication_status: published
publisher: Proceedings of the National Academy of Sciences
quality_controlled: '1'
scopus_import: '1'
status: public
title: RALF1 peptide triggers biphasic root growth inhibition upstream of auxin biosynthesis
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 119
year: '2022'
...
---
_id: '12053'
abstract:
- lang: eng
text: Strigolactones (SLs) are a class of phytohormones that regulate plant shoot
branching and adventitious root development. However, little is known regarding
the role of SLs in controlling the behavior of the smallest unit of the organism,
the single cell. Here, taking advantage of a classic single-cell model offered
by the cotton (Gossypium hirsutum) fiber cell, we show that SLs, whose biosynthesis
is fine-tuned by gibberellins (GAs), positively regulate cell elongation and cell
wall thickness by promoting the biosynthesis of very-long-chain fatty acids (VLCFAs)
and cellulose, respectively. Furthermore, we identified two layers of transcription
factors (TFs) involved in the hierarchical regulation of this GA-SL crosstalk.
The top-layer TF GROWTH-REGULATING FACTOR 4 (GhGRF4) directly activates expression
of the SL biosynthetic gene DWARF27 (D27) to increase SL accumulation in fiber
cells and GAs induce GhGRF4 expression. SLs induce the expression of four second-layer
TF genes (GhNAC100-2, GhBLH51, GhGT2, and GhB9SHZ1), which transmit SL signals
downstream to two ketoacyl-CoA synthase genes (KCS) and three cellulose synthase
(CesA) genes by directly activating their transcription. Finally, the KCS and
CesA enzymes catalyze the biosynthesis of very long chain fatty acids and cellulose,
respectively, to regulate development of high-grade cotton fibers. In addition
to providing a theoretical basis for cotton fiber improvement, our results shed
light on SL signaling in plant development at the single-cell level.
acknowledgement: This work was supported by the National Natural Science Foundation
of China (32070549), Shaanxi Youth Entrusted Talent Program (20190205), Fundamental
Research Funds for the Central Universities (GK202002005 and GK202201017), Young
Elite Scientists Sponsorship Program by China Association for Science and Technology
(CAST) (2019-2021QNRC001), State Key Laboratory of Cotton Biology Open Fund (CB2020A12
and CB2021A21) and FWF Stand-alone Project (P29988).
article_processing_charge: No
article_type: original
author:
- first_name: Z
full_name: Tian, Z
last_name: Tian
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: L
full_name: Zhu, L
last_name: Zhu
- first_name: B
full_name: Jiang, B
last_name: Jiang
- first_name: H
full_name: Wang, H
last_name: Wang
- first_name: R
full_name: Gao, R
last_name: Gao
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: G
full_name: Xiao, G
last_name: Xiao
citation:
ama: Tian Z, Zhang Y, Zhu L, et al. Strigolactones act downstream of gibberellins
to regulate fiber cell elongation and cell wall thickness in cotton (Gossypium
hirsutum). The Plant Cell. 2022;34(12):4816-4839. doi:10.1093/plcell/koac270
apa: Tian, Z., Zhang, Y., Zhu, L., Jiang, B., Wang, H., Gao, R., … Xiao, G. (2022).
Strigolactones act downstream of gibberellins to regulate fiber cell elongation
and cell wall thickness in cotton (Gossypium hirsutum). The Plant Cell.
Oxford University Press. https://doi.org/10.1093/plcell/koac270
chicago: Tian, Z, Yuzhou Zhang, L Zhu, B Jiang, H Wang, R Gao, Jiří Friml, and G
Xiao. “Strigolactones Act Downstream of Gibberellins to Regulate Fiber Cell Elongation
and Cell Wall Thickness in Cotton (Gossypium Hirsutum).” The Plant Cell.
Oxford University Press, 2022. https://doi.org/10.1093/plcell/koac270.
ieee: Z. Tian et al., “Strigolactones act downstream of gibberellins to regulate
fiber cell elongation and cell wall thickness in cotton (Gossypium hirsutum),”
The Plant Cell, vol. 34, no. 12. Oxford University Press, pp. 4816–4839,
2022.
ista: Tian Z, Zhang Y, Zhu L, Jiang B, Wang H, Gao R, Friml J, Xiao G. 2022. Strigolactones
act downstream of gibberellins to regulate fiber cell elongation and cell wall
thickness in cotton (Gossypium hirsutum). The Plant Cell. 34(12), 4816–4839.
mla: Tian, Z., et al. “Strigolactones Act Downstream of Gibberellins to Regulate
Fiber Cell Elongation and Cell Wall Thickness in Cotton (Gossypium Hirsutum).”
The Plant Cell, vol. 34, no. 12, Oxford University Press, 2022, pp. 4816–39,
doi:10.1093/plcell/koac270.
short: Z. Tian, Y. Zhang, L. Zhu, B. Jiang, H. Wang, R. Gao, J. Friml, G. Xiao,
The Plant Cell 34 (2022) 4816–4839.
date_created: 2022-09-07T14:19:39Z
date_published: 2022-12-01T00:00:00Z
date_updated: 2023-08-03T13:41:06Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1093/plcell/koac270
external_id:
isi:
- '000852753000001'
pmid:
- '36040191'
file:
- access_level: open_access
checksum: 1c606d9545f29dfca15235f69ad27b58
content_type: application/pdf
creator: dernst
date_created: 2023-01-20T08:29:12Z
date_updated: 2023-01-20T08:29:12Z
file_id: '12318'
file_name: 2022_PlantCell_Tian.pdf
file_size: 3282540
relation: main_file
success: 1
file_date_updated: 2023-01-20T08:29:12Z
has_accepted_license: '1'
intvolume: ' 34'
isi: 1
issue: '12'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
page: 4816-4839
pmid: 1
project:
- _id: 262EF96E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29988
name: RNA-directed DNA methylation in plant development
publication: The Plant Cell
publication_identifier:
eissn:
- 1532-298X
issn:
- 1040-4651
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
related_material:
link:
- relation: erratum
url: https://doi.org/10.1093/plcell/koac342
scopus_import: '1'
status: public
title: Strigolactones act downstream of gibberellins to regulate fiber cell elongation
and cell wall thickness in cotton (Gossypium hirsutum)
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 34
year: '2022'
...
---
_id: '12052'
abstract:
- lang: eng
text: Directionality in the intercellular transport of the plant hormone auxin is
determined by polar plasma membrane localization of PIN-FORMED (PIN) auxin transport
proteins. However, apart from PIN phosphorylation at conserved motifs, no further
determinants explicitly controlling polar PIN sorting decisions have been identified.
Here we present Arabidopsis WAVY GROWTH 3 (WAV3) and closely related RING-finger
E3 ubiquitin ligases, whose loss-of-function mutants show a striking apical-to-basal
polarity switch in PIN2 localization in root meristem cells. WAV3 E3 ligases function
as essential determinants for PIN polarity, acting independently from PINOID/WAG-dependent
PIN phosphorylation. They antagonize ectopic deposition of de novo synthesized
PIN proteins already immediately following completion of cell division, presumably
via preventing PIN sorting into basal, ARF GEF-mediated trafficking. Our findings
reveal an involvement of E3 ligases in the selective targeting of apically localized
PINs in higher plants.
acknowledgement: We would like to thank Tatsuo Sakai, Marcus Heisler, Toru Fujiwara,
Lucia Strader, Christian Hardtke, Malcolm Bennett, Claus Schwechheimer, Gerd Jürgens
and Remko Offringa for sharing published materials and Alba Grau Gimeno for support.
We are greatly indebted to Bert de Rybel for supporting N.K. and M.G. to work on
the final stages of manuscript preparation as postdocs in his laboratory. A full-length
SOR1 cDNA clone (J090099M14) was obtained from the National Agriculture and Food
Research Organization (NARO, Japan). Support by the Multiscale Imaging Core Facility
at the BOKU is greatly acknowledged. This work has been supported by grants from
the Austrian Science Fund (FWF P25931-B16; P31493-B25 to Christian Luschnig; I3630-B25
to Jiří Friml; P30850-B32 to Barbara Korbei) and from the Swiss National Funds (31003A-165877/1
to Markus Geisler) and the European Union’s Horizon 2020 research and innovation
program (Marie Skłodowska-Curie grant agreement No 885979 to Matouš Glanc).
article_number: '5147'
article_processing_charge: No
article_type: original
author:
- first_name: N
full_name: Konstantinova, N
last_name: Konstantinova
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: R
full_name: Keshkeih, R
last_name: Keshkeih
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: M
full_name: Di Donato, M
last_name: Di Donato
- first_name: K
full_name: Retzer, K
last_name: Retzer
- first_name: J
full_name: Moulinier-Anzola, J
last_name: Moulinier-Anzola
- first_name: M
full_name: Schwihla, M
last_name: Schwihla
- first_name: B
full_name: Korbei, B
last_name: Korbei
- first_name: M
full_name: Geisler, M
last_name: Geisler
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: C
full_name: Luschnig, C
last_name: Luschnig
citation:
ama: Konstantinova N, Hörmayer L, Glanc M, et al. WAVY GROWTH Arabidopsis E3 ubiquitin
ligases affect apical PIN sorting decisions. Nature Communications. 2022;13.
doi:10.1038/s41467-022-32888-8
apa: Konstantinova, N., Hörmayer, L., Glanc, M., Keshkeih, R., Tan, S., Di Donato,
M., … Luschnig, C. (2022). WAVY GROWTH Arabidopsis E3 ubiquitin ligases affect
apical PIN sorting decisions. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-022-32888-8
chicago: Konstantinova, N, Lukas Hörmayer, Matous Glanc, R Keshkeih, Shutang Tan,
M Di Donato, K Retzer, et al. “WAVY GROWTH Arabidopsis E3 Ubiquitin Ligases Affect
Apical PIN Sorting Decisions.” Nature Communications. Springer Nature,
2022. https://doi.org/10.1038/s41467-022-32888-8.
ieee: N. Konstantinova et al., “WAVY GROWTH Arabidopsis E3 ubiquitin ligases
affect apical PIN sorting decisions,” Nature Communications, vol. 13. Springer
Nature, 2022.
ista: Konstantinova N, Hörmayer L, Glanc M, Keshkeih R, Tan S, Di Donato M, Retzer
K, Moulinier-Anzola J, Schwihla M, Korbei B, Geisler M, Friml J, Luschnig C. 2022.
WAVY GROWTH Arabidopsis E3 ubiquitin ligases affect apical PIN sorting decisions.
Nature Communications. 13, 5147.
mla: Konstantinova, N., et al. “WAVY GROWTH Arabidopsis E3 Ubiquitin Ligases Affect
Apical PIN Sorting Decisions.” Nature Communications, vol. 13, 5147, Springer
Nature, 2022, doi:10.1038/s41467-022-32888-8.
short: N. Konstantinova, L. Hörmayer, M. Glanc, R. Keshkeih, S. Tan, M. Di Donato,
K. Retzer, J. Moulinier-Anzola, M. Schwihla, B. Korbei, M. Geisler, J. Friml,
C. Luschnig, Nature Communications 13 (2022).
date_created: 2022-09-07T14:19:26Z
date_published: 2022-09-01T00:00:00Z
date_updated: 2023-08-03T13:40:32Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41467-022-32888-8
external_id:
isi:
- '000848744900004'
pmid:
- '36050482'
file:
- access_level: open_access
checksum: 43336758c89cd6c045839089af070afe
content_type: application/pdf
creator: dernst
date_created: 2022-09-08T07:46:16Z
date_updated: 2022-09-08T07:46:16Z
file_id: '12063'
file_name: 2022_NatureCommunications_Konstantinova.pdf
file_size: 6678579
relation: main_file
success: 1
file_date_updated: 2022-09-08T07:46:16Z
has_accepted_license: '1'
intvolume: ' 13'
isi: 1
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: Nature Communications
publication_identifier:
issn:
- 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- relation: erratum
url: https://doi.org/10.1038/s41467-022-33198-9
status: public
title: WAVY GROWTH Arabidopsis E3 ubiquitin ligases affect apical PIN sorting decisions
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 13
year: '2022'
...
---
_id: '12054'
abstract:
- lang: eng
text: 'Polar auxin transport is unique to plants and coordinates their growth and
development1,2. The PIN-FORMED (PIN) auxin transporters exhibit highly asymmetrical
localizations at the plasma membrane and drive polar auxin transport3,4; however,
their structures and transport mechanisms remain largely unknown. Here, we report
three inward-facing conformation structures of Arabidopsis thaliana PIN1: the
apo state, bound to the natural auxin indole-3-acetic acid (IAA), and in complex
with the polar auxin transport inhibitor N-1-naphthylphthalamic acid (NPA). The
transmembrane domain of PIN1 shares a conserved NhaA fold5. In the substrate-bound
structure, IAA is coordinated by both hydrophobic stacking and hydrogen bonding.
NPA competes with IAA for the same site at the intracellular pocket, but with
a much higher affinity. These findings inform our understanding of the substrate
recognition and transport mechanisms of PINs and set up a framework for future
research on directional auxin transport, one of the most crucial processes underlying
plant development.'
acknowledgement: We thank the Cryo-EM Center of the University of Science and Technology
of China (USTC) and the Center for Biological Imaging (CBI), Institute of Biophysics,
Chinese Academy of Science, for the EM facility support; we thank B. Zhu, X. Huang
and all the other staff members for their technical support on cryo-EM data collection.
We thank J. Ren for his technical support with the transport assays and M. Seeger
for providing the sybody libraries. This work was supported by the Strategic Priority
Research Program of Chinese Academy of Sciences (XDB 37020204 to D.L. and XDB37020103
to Linfeng Sun), National Natural Science Foundation of China (82151215 and 31870726
to D.L., 31900885 to X.L., and 31870732 to Linfeng Sun), Natural Science Foundation
of Anhui Province (2008085MC90 to X.L. and 2008085J15 to Linfeng Sun), the Fundamental
Research Funds for the Central Universities (WK9100000031 to Linfeng Sun), and the
USTC Research Funds of the Double First-Class Initiative (YD9100002004 to Linfeng
Sun). Linfeng Sun is supported by an Outstanding Young Scholar Award from the Qiu
Shi Science and Technologies Foundation, and a Young Scholar Award from the Cyrus
Tang Foundation.
article_processing_charge: No
article_type: original
author:
- first_name: Z
full_name: Yang, Z
last_name: Yang
- first_name: J
full_name: Xia, J
last_name: Xia
- first_name: J
full_name: Hong, J
last_name: Hong
- first_name: C
full_name: Zhang, C
last_name: Zhang
- first_name: H
full_name: Wei, H
last_name: Wei
- first_name: W
full_name: Ying, W
last_name: Ying
- first_name: C
full_name: Sun, C
last_name: Sun
- first_name: L
full_name: Sun, L
last_name: Sun
- first_name: Y
full_name: Mao, Y
last_name: Mao
- first_name: Y
full_name: Gao, Y
last_name: Gao
- first_name: S
full_name: Tan, S
last_name: Tan
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: D
full_name: Li, D
last_name: Li
- first_name: X
full_name: Liu, X
last_name: Liu
- first_name: L
full_name: Sun, L
last_name: Sun
citation:
ama: Yang Z, Xia J, Hong J, et al. Structural insights into auxin recognition and
efflux by Arabidopsis PIN1. Nature. 2022;609(7927):611-615. doi:10.1038/s41586-022-05143-9
apa: Yang, Z., Xia, J., Hong, J., Zhang, C., Wei, H., Ying, W., … Sun, L. (2022).
Structural insights into auxin recognition and efflux by Arabidopsis PIN1. Nature.
Springer Nature. https://doi.org/10.1038/s41586-022-05143-9
chicago: Yang, Z, J Xia, J Hong, C Zhang, H Wei, W Ying, C Sun, et al. “Structural
Insights into Auxin Recognition and Efflux by Arabidopsis PIN1.” Nature.
Springer Nature, 2022. https://doi.org/10.1038/s41586-022-05143-9.
ieee: Z. Yang et al., “Structural insights into auxin recognition and efflux
by Arabidopsis PIN1,” Nature, vol. 609, no. 7927. Springer Nature, pp.
611–615, 2022.
ista: Yang Z, Xia J, Hong J, Zhang C, Wei H, Ying W, Sun C, Sun L, Mao Y, Gao Y,
Tan S, Friml J, Li D, Liu X, Sun L. 2022. Structural insights into auxin recognition
and efflux by Arabidopsis PIN1. Nature. 609(7927), 611–615.
mla: Yang, Z., et al. “Structural Insights into Auxin Recognition and Efflux by
Arabidopsis PIN1.” Nature, vol. 609, no. 7927, Springer Nature, 2022, pp.
611–15, doi:10.1038/s41586-022-05143-9.
short: Z. Yang, J. Xia, J. Hong, C. Zhang, H. Wei, W. Ying, C. Sun, L. Sun, Y. Mao,
Y. Gao, S. Tan, J. Friml, D. Li, X. Liu, L. Sun, Nature 609 (2022) 611–615.
date_created: 2022-09-07T14:19:52Z
date_published: 2022-08-02T00:00:00Z
date_updated: 2023-08-03T13:41:44Z
day: '02'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41586-022-05143-9
external_id:
isi:
- '000848082900002'
pmid:
- '35917925'
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publication_identifier:
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issn:
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publication_status: published
publisher: Springer Nature
quality_controlled: '1'
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title: Structural insights into auxin recognition and efflux by Arabidopsis PIN1
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 609
year: '2022'
...
---
_id: '12121'
abstract:
- lang: eng
text: Autophagosomes are double-membraned vesicles that traffic harmful or unwanted
cellular macromolecules to the vacuole for recycling. Although autophagosome biogenesis
has been extensively studied, autophagosome maturation, i.e., delivery and fusion
with the vacuole, remains largely unknown in plants. Here, we have identified
an autophagy adaptor, CFS1, that directly interacts with the autophagosome marker
ATG8 and localizes on both membranes of the autophagosome. Autophagosomes form
normally in Arabidopsis thaliana cfs1 mutants, but their delivery to the vacuole
is disrupted. CFS1’s function is evolutionarily conserved in plants, as it also
localizes to the autophagosomes and plays a role in autophagic flux in the liverwort
Marchantia polymorpha. CFS1 regulates autophagic flux by bridging autophagosomes
with the multivesicular body-localized ESCRT-I component VPS23A, leading to the
formation of amphisomes. Similar to CFS1-ATG8 interaction, disrupting the CFS1-VPS23A
interaction blocks autophagic flux and renders plants sensitive to nitrogen starvation.
Altogether, our results reveal a conserved vacuolar sorting hub that regulates
autophagic flux in plants.
acknowledgement: "We thank Suayip Ustün, Karin Schumacher, Erika Isono, Gerd Juergens,
Takashi Ueda, Daniel Hofius, and Liwen Jiang for sharing published materials.\r\nWe
acknowledge funding from Austrian Academy of Sciences, Austrian Science Fund (FWF,
P 32355, P 34944), Austrian Science Fund (FWF-SFB F79), Vienna Science and Technology\r\nFund
(WWTF, LS17-047) to Y. Dagdas; Austrian Academy of Sciences DOC Fellowship to J.
Zhao, Marie Curie VIP2 Fellowship to J.C. De La Concepcion and M. Clavel; Hong Kong
Research Grant Council (GRF14121019, 14113921, AoE/M-05/12, C4002-17G) to B.-H.
Kang. We thank Vienna Biocenter Core Facilities (VBCF) Protein Chemistry, Biooptics,
Plant Sciences, Molecular Biology, and Protein Technologies. We thank J. Matthew
Watson\r\nand members of the Dagdas lab for the critical reading and editing of
the manuscript."
article_number: e202203139
article_processing_charge: No
article_type: original
author:
- first_name: Jierui
full_name: Zhao, Jierui
last_name: Zhao
- first_name: Mai Thu
full_name: Bui, Mai Thu
last_name: Bui
- first_name: Juncai
full_name: Ma, Juncai
last_name: Ma
- first_name: Fabian
full_name: Künzl, Fabian
last_name: Künzl
- first_name: Lorenzo
full_name: Picchianti, Lorenzo
last_name: Picchianti
- first_name: Juan Carlos
full_name: De La Concepcion, Juan Carlos
last_name: De La Concepcion
- first_name: Yixuan
full_name: Chen, Yixuan
last_name: Chen
- first_name: Sofia
full_name: Petsangouraki, Sofia
last_name: Petsangouraki
- first_name: Azadeh
full_name: Mohseni, Azadeh
last_name: Mohseni
- first_name: Marta
full_name: García-Leon, Marta
last_name: García-Leon
- first_name: Marta Salas
full_name: Gomez, Marta Salas
last_name: Gomez
- first_name: Caterina
full_name: Giannini, Caterina
id: e3fdddd5-f6e0-11ea-865d-ca99ee6367f4
last_name: Giannini
- first_name: Dubois
full_name: Gwennogan, Dubois
last_name: Gwennogan
- first_name: Roksolana
full_name: Kobylinska, Roksolana
last_name: Kobylinska
- first_name: Marion
full_name: Clavel, Marion
last_name: Clavel
- first_name: Swen
full_name: Schellmann, Swen
last_name: Schellmann
- first_name: Yvon
full_name: Jaillais, Yvon
last_name: Jaillais
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Byung-Ho
full_name: Kang, Byung-Ho
last_name: Kang
- first_name: Yasin
full_name: Dagdas, Yasin
last_name: Dagdas
citation:
ama: Zhao J, Bui MT, Ma J, et al. Plant autophagosomes mature into amphisomes prior
to their delivery to the central vacuole. Journal of Cell Biology. 2022;221(12).
doi:10.1083/jcb.202203139
apa: Zhao, J., Bui, M. T., Ma, J., Künzl, F., Picchianti, L., De La Concepcion,
J. C., … Dagdas, Y. (2022). Plant autophagosomes mature into amphisomes prior
to their delivery to the central vacuole. Journal of Cell Biology. Rockefeller
University Press. https://doi.org/10.1083/jcb.202203139
chicago: Zhao, Jierui, Mai Thu Bui, Juncai Ma, Fabian Künzl, Lorenzo Picchianti,
Juan Carlos De La Concepcion, Yixuan Chen, et al. “Plant Autophagosomes Mature
into Amphisomes Prior to Their Delivery to the Central Vacuole.” Journal of
Cell Biology. Rockefeller University Press, 2022. https://doi.org/10.1083/jcb.202203139.
ieee: J. Zhao et al., “Plant autophagosomes mature into amphisomes prior
to their delivery to the central vacuole,” Journal of Cell Biology, vol.
221, no. 12. Rockefeller University Press, 2022.
ista: Zhao J, Bui MT, Ma J, Künzl F, Picchianti L, De La Concepcion JC, Chen Y,
Petsangouraki S, Mohseni A, García-Leon M, Gomez MS, Giannini C, Gwennogan D,
Kobylinska R, Clavel M, Schellmann S, Jaillais Y, Friml J, Kang B-H, Dagdas Y.
2022. Plant autophagosomes mature into amphisomes prior to their delivery to the
central vacuole. Journal of Cell Biology. 221(12), e202203139.
mla: Zhao, Jierui, et al. “Plant Autophagosomes Mature into Amphisomes Prior to
Their Delivery to the Central Vacuole.” Journal of Cell Biology, vol. 221,
no. 12, e202203139, Rockefeller University Press, 2022, doi:10.1083/jcb.202203139.
short: J. Zhao, M.T. Bui, J. Ma, F. Künzl, L. Picchianti, J.C. De La Concepcion,
Y. Chen, S. Petsangouraki, A. Mohseni, M. García-Leon, M.S. Gomez, C. Giannini,
D. Gwennogan, R. Kobylinska, M. Clavel, S. Schellmann, Y. Jaillais, J. Friml,
B.-H. Kang, Y. Dagdas, Journal of Cell Biology 221 (2022).
date_created: 2023-01-12T11:57:10Z
date_published: 2022-12-01T00:00:00Z
date_updated: 2023-08-03T14:20:15Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1083/jcb.202203139
external_id:
isi:
- '000932958800001'
pmid:
- '36260289'
file:
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checksum: 050b5cc4b25e6b94fe3e3cbfe0f5c06b
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creator: dernst
date_created: 2023-01-23T10:30:11Z
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file_date_updated: 2023-01-23T10:30:11Z
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intvolume: ' 221'
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issue: '12'
keyword:
- Cell Biology
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
pmid: 1
publication: Journal of Cell Biology
publication_identifier:
eissn:
- 1540-8140
issn:
- 0021-9525
publication_status: published
publisher: Rockefeller University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Plant autophagosomes mature into amphisomes prior to their delivery to the
central vacuole
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 221
year: '2022'
...