---
_id: '1221'
abstract:
- lang: eng
text: The Auxin Binding Protein 1 (ABP1) is one of the most studied proteins in
plants. Since decades ago, it has been the prime receptor candidate for the plant
hormone auxin with a plethora of described functions in auxin signaling and development.
The developmental importance of ABP1 has recently been questioned by identification
of Arabidopsis thaliana abp1 knock-out alleles that show no obvious phenotypes
under normal growth conditions. In this study, we examined the contradiction between
the normal growth and development of the abp1 knock-outs and the strong morphological
defects observed in three different ethanol-inducible abp1 knock-down mutants
( abp1-AS, SS12K, SS12S). By analyzing segregating populations of abp1 knock-out
vs. abp1 knock-down crosses we show that the strong morphological defects that
were believed to be the result of conditional down-regulation of ABP1 can be reproduced
also in the absence of the functional ABP1 protein. This data suggests that the
phenotypes in abp1 knock-down lines are due to the off-target effects and asks
for further reflections on the biological function of ABP1 or alternative explanations
for the missing phenotypic defects in the abp1 loss-of-function alleles.
acknowledgement: "This work was supported by ERC Independent Research grant (ERC-2011-StG-20101109-PSDP
to JF). JM internship was supported by the grant “Action Austria – Slovakia”. MG
was supported by the scholarship \"Stipendien der Stipendienstiftung der Republik
Österreich\". Work by EH and CPR were supported by ANR blanc ANR-14-CE11-0018. We
would like to thank Mark Estelle and Yunde Zhao for provid\r\n-\r\ning \r\nabp1-c1\r\n,
\r\nabp1-TD1 \r\nand \r\nabp1-WTc1 \r\nseeds. We thank Emeline \r\nHuault for technical
assistance."
article_number: '86'
article_processing_charge: No
article_type: original
author:
- first_name: Jaroslav
full_name: Michalko, Jaroslav
id: 483727CA-F248-11E8-B48F-1D18A9856A87
last_name: Michalko
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: Catherine
full_name: Perrot Rechenmann, Catherine
last_name: Perrot Rechenmann
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Michalko J, Glanc M, Perrot Rechenmann C, Friml J. Strong morphological defects
in conditional Arabidopsis abp1 knock-down mutants generated in absence of functional
ABP1 protein. F1000 Research . 2016;5. doi:10.12688/f1000research.7654.1
apa: Michalko, J., Glanc, M., Perrot Rechenmann, C., & Friml, J. (2016). Strong
morphological defects in conditional Arabidopsis abp1 knock-down mutants generated
in absence of functional ABP1 protein. F1000 Research . F1000 Research.
https://doi.org/10.12688/f1000research.7654.1
chicago: Michalko, Jaroslav, Matous Glanc, Catherine Perrot Rechenmann, and Jiří
Friml. “Strong Morphological Defects in Conditional Arabidopsis Abp1 Knock-down
Mutants Generated in Absence of Functional ABP1 Protein.” F1000 Research .
F1000 Research, 2016. https://doi.org/10.12688/f1000research.7654.1.
ieee: J. Michalko, M. Glanc, C. Perrot Rechenmann, and J. Friml, “Strong morphological
defects in conditional Arabidopsis abp1 knock-down mutants generated in absence
of functional ABP1 protein,” F1000 Research , vol. 5. F1000 Research, 2016.
ista: Michalko J, Glanc M, Perrot Rechenmann C, Friml J. 2016. Strong morphological
defects in conditional Arabidopsis abp1 knock-down mutants generated in absence
of functional ABP1 protein. F1000 Research . 5, 86.
mla: Michalko, Jaroslav, et al. “Strong Morphological Defects in Conditional Arabidopsis
Abp1 Knock-down Mutants Generated in Absence of Functional ABP1 Protein.” F1000
Research , vol. 5, 86, F1000 Research, 2016, doi:10.12688/f1000research.7654.1.
short: J. Michalko, M. Glanc, C. Perrot Rechenmann, J. Friml, F1000 Research 5
(2016).
date_created: 2018-12-11T11:50:47Z
date_published: 2016-01-20T00:00:00Z
date_updated: 2022-03-24T09:12:49Z
day: '20'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.12688/f1000research.7654.1
ec_funded: 1
file:
- access_level: open_access
checksum: c9e50bb6096a7ba4a832969935820f19
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:15:33Z
date_updated: 2020-07-14T12:44:39Z
file_id: '5154'
file_name: IST-2016-711-v1+1_770cf1e0-612f-4e85-a500-54b6349fbbab_7654_-_jaroslav_michalko.pdf
file_size: 2990459
relation: main_file
file_date_updated: 2020-07-14T12:44:39Z
has_accepted_license: '1'
intvolume: ' 5'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: 'F1000 Research '
publication_status: published
publisher: F1000 Research
publist_id: '6113'
pubrep_id: '711'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Strong morphological defects in conditional Arabidopsis abp1 knock-down mutants
generated in absence of functional ABP1 protein
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 5
year: '2016'
...
---
_id: '1238'
abstract:
- lang: eng
text: The dynamic localization of endosomal compartments labeled with targeted fluorescent
protein tags is routinely followed by time lapse fluorescence microscopy approaches
and single particle tracking algorithms. In this way trajectories of individual
endosomes can be mapped and linked to physiological processes as cell growth.
However, other aspects of dynamic behavior including endosomal interactions are
difficult to follow in this manner. Therefore, we characterized the localization
and dynamic properties of early and late endosomes throughout the entire course
of root hair formation by means of spinning disc time lapse imaging and post-acquisition
automated multitracking and quantitative analysis. Our results show differential
motile behavior of early and late endosomes and interactions of late endosomes
that may be specified to particular root hair domains. Detailed data analysis
revealed a particular transient interaction between late endosomes—termed herein
as dancing-endosomes—which is not concluding to vesicular fusion. Endosomes preferentially
located in the root hair tip interacted as dancing-endosomes and traveled short
distances during this interaction. Finally, sizes of early and late endosomes
were addressed by means of super-resolution structured illumination microscopy
(SIM) to corroborate measurements on the spinning disc. This is a first study
providing quantitative microscopic data on dynamic spatio-temporal interactions
of endosomes during root hair tip growth.
acknowledgement: "This work was supported by National Program for Sustainability I
(grant no. LO1204) provided by the Czech Ministry of Education and by Institutional
Fund of Palacký University Olomouc (GK and OŠ).\r\nWe thank Sabine Fischer for help
with the statistics."
article_number: '1262'
author:
- first_name: Daniel
full_name: Von Wangenheim, Daniel
id: 49E91952-F248-11E8-B48F-1D18A9856A87
last_name: Von Wangenheim
orcid: 0000-0002-6862-1247
- first_name: Amparo
full_name: Rosero, Amparo
last_name: Rosero
- first_name: George
full_name: Komis, George
last_name: Komis
- first_name: Olga
full_name: Šamajová, Olga
last_name: Šamajová
- first_name: Miroslav
full_name: Ovečka, Miroslav
last_name: Ovečka
- first_name: Boris
full_name: Voigt, Boris
last_name: Voigt
- first_name: Jozef
full_name: Šamaj, Jozef
last_name: Šamaj
citation:
ama: von Wangenheim D, Rosero A, Komis G, et al. Endosomal interactions during root
hair growth. Frontiers in Plant Science. 2016;6(JAN2016). doi:10.3389/fpls.2015.01262
apa: von Wangenheim, D., Rosero, A., Komis, G., Šamajová, O., Ovečka, M., Voigt,
B., & Šamaj, J. (2016). Endosomal interactions during root hair growth. Frontiers
in Plant Science. Frontiers Research Foundation. https://doi.org/10.3389/fpls.2015.01262
chicago: Wangenheim, Daniel von, Amparo Rosero, George Komis, Olga Šamajová, Miroslav
Ovečka, Boris Voigt, and Jozef Šamaj. “Endosomal Interactions during Root Hair
Growth.” Frontiers in Plant Science. Frontiers Research Foundation, 2016.
https://doi.org/10.3389/fpls.2015.01262.
ieee: D. von Wangenheim et al., “Endosomal interactions during root hair
growth,” Frontiers in Plant Science, vol. 6, no. JAN2016. Frontiers Research
Foundation, 2016.
ista: von Wangenheim D, Rosero A, Komis G, Šamajová O, Ovečka M, Voigt B, Šamaj
J. 2016. Endosomal interactions during root hair growth. Frontiers in Plant Science.
6(JAN2016), 1262.
mla: von Wangenheim, Daniel, et al. “Endosomal Interactions during Root Hair Growth.”
Frontiers in Plant Science, vol. 6, no. JAN2016, 1262, Frontiers Research
Foundation, 2016, doi:10.3389/fpls.2015.01262.
short: D. von Wangenheim, A. Rosero, G. Komis, O. Šamajová, M. Ovečka, B. Voigt,
J. Šamaj, Frontiers in Plant Science 6 (2016).
date_created: 2018-12-11T11:50:53Z
date_published: 2016-01-29T00:00:00Z
date_updated: 2021-01-12T06:49:18Z
day: '29'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.3389/fpls.2015.01262
file:
- access_level: open_access
checksum: 3127eab844d53564bf47e2b6b42f1ca0
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:09:36Z
date_updated: 2020-07-14T12:44:41Z
file_id: '4760'
file_name: IST-2016-710-v1+1_fpls-06-01262.pdf
file_size: 1640550
relation: main_file
file_date_updated: 2020-07-14T12:44:41Z
has_accepted_license: '1'
intvolume: ' 6'
issue: JAN2016
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
publication: Frontiers in Plant Science
publication_status: published
publisher: Frontiers Research Foundation
publist_id: '6094'
pubrep_id: '710'
quality_controlled: '1'
scopus_import: 1
status: public
title: Endosomal interactions during root hair growth
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 6
year: '2016'
...
---
_id: '1247'
abstract:
- lang: eng
text: The shaping of organs in plants depends on the intercellular flow of the phytohormone
auxin, of which the directional signaling is determined by the polar subcellular
localization of PIN-FORMED (PIN) auxin transport proteins. Phosphorylation dynamics
of PIN proteins are affected by the protein phosphatase 2A (PP2A) and the PINOID
kinase, which act antagonistically to mediate their apical-basal polar delivery.
Here, we identified the ROTUNDA3 (RON3) protein as a regulator of the PP2A phosphatase
activity in Arabidopsis thaliana. The RON3 gene was map-based cloned starting
from the ron3-1 leaf mutant and found to be a unique, plant-specific gene coding
for a protein with high and dispersed proline content. The ron3-1 and ron3-2 mutant
phenotypes [i.e., reduced apical dominance, primary root length, lateral root
emergence, and growth; increased ectopic stages II, IV, and V lateral root primordia;
decreased auxin maxima in indole-3-acetic acid (IAA)-treated root apical meristems;
hypergravitropic root growth and response; increased IAA levels in shoot apices;
and reduced auxin accumulation in root meristems] support a role for RON3 in auxin
biology. The affinity-purified PP2A complex with RON3 as bait suggested that RON3
might act in PIN transporter trafficking. Indeed, pharmacological interference
with vesicle trafficking processes revealed that single ron3-2 and double ron3-2
rcn1 mutants have altered PIN polarity and endocytosis in specific cells. Our
data indicate that RON3 contributes to auxin-mediated development by playing a
role in PIN recycling and polarity establishment through regulation of the PP2A
complex activity.
acknowledgement: "This work was supported by the Ghent University Special Research
Fund (M.K.), the European Research Council (Project ERC-2011-StG-20101109-PSDP)
(to J.F.), and the Körber European Science Foun-\r\ndation (J.F.). S.D.G. is indebted
to the Agency for Science and Technology for\r\na predoctoral fellowship."
author:
- first_name: Michael
full_name: Karampelias, Michael
last_name: Karampelias
- first_name: Pia
full_name: Neyt, Pia
last_name: Neyt
- first_name: Steven
full_name: De Groeve, Steven
last_name: De Groeve
- first_name: Stijn
full_name: Aesaert, Stijn
last_name: Aesaert
- first_name: Griet
full_name: Coussens, Griet
last_name: Coussens
- first_name: Jakub
full_name: Rolčík, Jakub
last_name: Rolčík
- first_name: Leonardo
full_name: Bruno, Leonardo
last_name: Bruno
- first_name: Nancy
full_name: De Winne, Nancy
last_name: De Winne
- first_name: Annemie
full_name: Van Minnebruggen, Annemie
last_name: Van Minnebruggen
- first_name: Marc
full_name: Van Montagu, Marc
last_name: Van Montagu
- first_name: Maria
full_name: Ponce, Maria
last_name: Ponce
- first_name: José
full_name: Micol, José
last_name: Micol
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Geert
full_name: De Jaeger, Geert
last_name: De Jaeger
- first_name: Mieke
full_name: Van Lijsebettens, Mieke
last_name: Van Lijsebettens
citation:
ama: Karampelias M, Neyt P, De Groeve S, et al. ROTUNDA3 function in plant development
by phosphatase 2A-mediated regulation of auxin transporter recycling. PNAS.
2016;113(10):2768-2773. doi:10.1073/pnas.1501343112
apa: Karampelias, M., Neyt, P., De Groeve, S., Aesaert, S., Coussens, G., Rolčík,
J., … Van Lijsebettens, M. (2016). ROTUNDA3 function in plant development by phosphatase
2A-mediated regulation of auxin transporter recycling. PNAS. National Academy
of Sciences. https://doi.org/10.1073/pnas.1501343112
chicago: Karampelias, Michael, Pia Neyt, Steven De Groeve, Stijn Aesaert, Griet
Coussens, Jakub Rolčík, Leonardo Bruno, et al. “ROTUNDA3 Function in Plant Development
by Phosphatase 2A-Mediated Regulation of Auxin Transporter Recycling.” PNAS.
National Academy of Sciences, 2016. https://doi.org/10.1073/pnas.1501343112.
ieee: M. Karampelias et al., “ROTUNDA3 function in plant development by phosphatase
2A-mediated regulation of auxin transporter recycling,” PNAS, vol. 113,
no. 10. National Academy of Sciences, pp. 2768–2773, 2016.
ista: Karampelias M, Neyt P, De Groeve S, Aesaert S, Coussens G, Rolčík J, Bruno
L, De Winne N, Van Minnebruggen A, Van Montagu M, Ponce M, Micol J, Friml J, De
Jaeger G, Van Lijsebettens M. 2016. ROTUNDA3 function in plant development by
phosphatase 2A-mediated regulation of auxin transporter recycling. PNAS. 113(10),
2768–2773.
mla: Karampelias, Michael, et al. “ROTUNDA3 Function in Plant Development by Phosphatase
2A-Mediated Regulation of Auxin Transporter Recycling.” PNAS, vol. 113,
no. 10, National Academy of Sciences, 2016, pp. 2768–73, doi:10.1073/pnas.1501343112.
short: M. Karampelias, P. Neyt, S. De Groeve, S. Aesaert, G. Coussens, J. Rolčík,
L. Bruno, N. De Winne, A. Van Minnebruggen, M. Van Montagu, M. Ponce, J. Micol,
J. Friml, G. De Jaeger, M. Van Lijsebettens, PNAS 113 (2016) 2768–2773.
date_created: 2018-12-11T11:50:56Z
date_published: 2016-03-08T00:00:00Z
date_updated: 2021-01-12T06:49:22Z
day: '08'
department:
- _id: JiFr
doi: 10.1073/pnas.1501343112
ec_funded: 1
intvolume: ' 113'
issue: '10'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4791031/
month: '03'
oa: 1
oa_version: Submitted Version
page: 2768 - 2773
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '6081'
quality_controlled: '1'
scopus_import: 1
status: public
title: ROTUNDA3 function in plant development by phosphatase 2A-mediated regulation
of auxin transporter recycling
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 113
year: '2016'
...
---
_id: '1251'
abstract:
- lang: eng
text: Plant growth and architecture is regulated by the polar distribution of the
hormone auxin. Polarity and flexibility of this process is provided by constant
cycling of auxin transporter vesicles along actin filaments, coordinated by a
positive auxinactin feedback loop. Both polar auxin transport and vesicle cycling
are inhibited by synthetic auxin transport inhibitors, such as 1-Nnaphthylphthalamic
acid (NPA), counteracting the effect of auxin; however, underlying targets and
mechanisms are unclear. Using NMR, we map the NPA binding surface on the Arabidopsis
thaliana ABCB chaperone TWISTED DWARF1 (TWD1).We identify ACTIN7 as a relevant,
although likely indirect, TWD1 interactor, and show TWD1-dependent regulation
of actin filament organization and dynamics and that TWD1 is required for NPA-mediated
actin cytoskeleton remodeling. The TWD1-ACTIN7 axis controls plasma membrane presence
of efflux transporters, and as a consequence act7 and twd1 share developmental
and physiological phenotypes indicative of defects in auxin transport. These can
be phenocopied by NPA treatment or by chemical actin (de)stabilization. We provide
evidence that TWD1 determines downstreamlocations of auxin efflux transporters
by adjusting actin filament debundling and dynamizing processes and mediating
NPA action on the latter. This function appears to be evolutionary conserved since
TWD1 expression in budding yeast alters actin polarization and cell polarity and
provides NPA sensitivity.
acknowledgement: ' This work was supported by grants from the European Social Fund
(CZ.1.07/2.3.00/20.0043), the Czech Science Foundation GAČR (GA13-40637S) to J.F.
and M.Z., the Ministry of Education, Youth, and Sports of the Czech Republic under
the project CEITEC 2020 (LQ1601) to M.Z., the Ministry for Higher Education and
Research of Luxembourg (REC-LOCM-20140703) to C.T., the Partial Funding Program
for Short Stays Abroad of CONICET Argentina (to N.I.B.), Swiss National Funds, the
Pool de Recherche of the University of Fribourg, and the Novartis Foundation (all
to M.G.). '
author:
- first_name: Jinsheng
full_name: Zhu, Jinsheng
last_name: Zhu
- first_name: Aurélien
full_name: Bailly, Aurélien
last_name: Bailly
- first_name: Marta
full_name: Zwiewka, Marta
last_name: Zwiewka
- first_name: Valpuri
full_name: Sovero, Valpuri
last_name: Sovero
- first_name: Martin
full_name: Di Donato, Martin
last_name: Di Donato
- first_name: Pei
full_name: Ge, Pei
last_name: Ge
- first_name: Jacqueline
full_name: Oehri, Jacqueline
last_name: Oehri
- first_name: Bibek
full_name: Aryal, Bibek
last_name: Aryal
- first_name: Pengchao
full_name: Hao, Pengchao
last_name: Hao
- first_name: Miriam
full_name: Linnert, Miriam
last_name: Linnert
- first_name: Noelia
full_name: Burgardt, Noelia
last_name: Burgardt
- first_name: Christian
full_name: Lücke, Christian
last_name: Lücke
- first_name: Matthias
full_name: Weiwad, Matthias
last_name: Weiwad
- first_name: Max
full_name: Michel, Max
last_name: Michel
- first_name: Oliver
full_name: Weiergräber, Oliver
last_name: Weiergräber
- first_name: Stephan
full_name: Pollmann, Stephan
last_name: Pollmann
- first_name: Elisa
full_name: Azzarello, Elisa
last_name: Azzarello
- first_name: Stefano
full_name: Mancuso, Stefano
last_name: Mancuso
- first_name: Noel
full_name: Ferro, Noel
last_name: Ferro
- first_name: Yoichiro
full_name: Fukao, Yoichiro
last_name: Fukao
- first_name: Céline
full_name: Hoffmann, Céline
last_name: Hoffmann
- first_name: Roland
full_name: Wedlich Söldner, Roland
last_name: Wedlich Söldner
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Clément
full_name: Thomas, Clément
last_name: Thomas
- first_name: Markus
full_name: Geisler, Markus
last_name: Geisler
citation:
ama: Zhu J, Bailly A, Zwiewka M, et al. TWISTED DWARF1 mediates the action of auxin
transport inhibitors on actin cytoskeleton dynamics. Plant Cell. 2016;28(4):930-948.
doi:10.1105/tpc.15.00726
apa: Zhu, J., Bailly, A., Zwiewka, M., Sovero, V., Di Donato, M., Ge, P., … Geisler,
M. (2016). TWISTED DWARF1 mediates the action of auxin transport inhibitors on
actin cytoskeleton dynamics. Plant Cell. American Society of Plant Biologists.
https://doi.org/10.1105/tpc.15.00726
chicago: Zhu, Jinsheng, Aurélien Bailly, Marta Zwiewka, Valpuri Sovero, Martin Di
Donato, Pei Ge, Jacqueline Oehri, et al. “TWISTED DWARF1 Mediates the Action of
Auxin Transport Inhibitors on Actin Cytoskeleton Dynamics.” Plant Cell.
American Society of Plant Biologists, 2016. https://doi.org/10.1105/tpc.15.00726.
ieee: J. Zhu et al., “TWISTED DWARF1 mediates the action of auxin transport
inhibitors on actin cytoskeleton dynamics,” Plant Cell, vol. 28, no. 4.
American Society of Plant Biologists, pp. 930–948, 2016.
ista: Zhu J, Bailly A, Zwiewka M, Sovero V, Di Donato M, Ge P, Oehri J, Aryal B,
Hao P, Linnert M, Burgardt N, Lücke C, Weiwad M, Michel M, Weiergräber O, Pollmann
S, Azzarello E, Mancuso S, Ferro N, Fukao Y, Hoffmann C, Wedlich Söldner R, Friml
J, Thomas C, Geisler M. 2016. TWISTED DWARF1 mediates the action of auxin transport
inhibitors on actin cytoskeleton dynamics. Plant Cell. 28(4), 930–948.
mla: Zhu, Jinsheng, et al. “TWISTED DWARF1 Mediates the Action of Auxin Transport
Inhibitors on Actin Cytoskeleton Dynamics.” Plant Cell, vol. 28, no. 4,
American Society of Plant Biologists, 2016, pp. 930–48, doi:10.1105/tpc.15.00726.
short: J. Zhu, A. Bailly, M. Zwiewka, V. Sovero, M. Di Donato, P. Ge, J. Oehri,
B. Aryal, P. Hao, M. Linnert, N. Burgardt, C. Lücke, M. Weiwad, M. Michel, O.
Weiergräber, S. Pollmann, E. Azzarello, S. Mancuso, N. Ferro, Y. Fukao, C. Hoffmann,
R. Wedlich Söldner, J. Friml, C. Thomas, M. Geisler, Plant Cell 28 (2016) 930–948.
date_created: 2018-12-11T11:50:57Z
date_published: 2016-04-01T00:00:00Z
date_updated: 2021-01-12T06:49:24Z
day: '01'
department:
- _id: JiFr
doi: 10.1105/tpc.15.00726
intvolume: ' 28'
issue: '4'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4863381/
month: '04'
oa: 1
oa_version: Submitted Version
page: 930 - 948
publication: Plant Cell
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '6078'
quality_controlled: '1'
scopus_import: 1
status: public
title: TWISTED DWARF1 mediates the action of auxin transport inhibitors on actin cytoskeleton
dynamics
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 28
year: '2016'
...
---
_id: '1264'
abstract:
- lang: eng
text: n contrast with the wealth of recent reports about the function of μ-adaptins
and clathrin adaptor protein (AP) complexes, there is very little information
about the motifs that determine the sorting of membrane proteins within clathrin-coated
vesicles in plants. Here, we investigated putative sorting signals in the large
cytosolic loop of the Arabidopsis (Arabidopsis thaliana) PIN-FORMED1 (PIN1) auxin
transporter, which are involved in binding μ-adaptins and thus in PIN1 trafficking
and localization. We found that Phe-165 and Tyr-280, Tyr-328, and Tyr-394 are
involved in the binding of different μ-adaptins in vitro. However, only Phe-165,
which binds μA(μ2)- and μD(μ3)-adaptin, was found to be essential for PIN1 trafficking
and localization in vivo. The PIN1:GFP-F165A mutant showed reduced endocytosis
but also localized to intracellular structures containing several layers of membranes
and endoplasmic reticulum (ER) markers, suggesting that they correspond to ER
or ER-derived membranes. While PIN1:GFP localized normally in a μA (μ2)-adaptin
mutant, it accumulated in big intracellular structures containing LysoTracker
in a μD (μ3)-adaptin mutant, consistent with previous results obtained with mutants
of other subunits of the AP-3 complex. Our data suggest that Phe-165, through
the binding of μA (μ2)- and μD (μ3)-adaptin, is important for PIN1 endocytosis
and for PIN1 trafficking along the secretory pathway, respectively.
acknowledgement: "We thank Dr. R. Offringa (Leiden University) for providing the GST-\r\nPIN-CL
construct; Sandra Richter and Gerd Jurgens (University of Tübin-\r\ngen) for providing
the estradiol-inducible PIN1-RFP construct and the\r\ngnl1 mutant expressing BFA-sensitive
GNL1; F.J. Santonja (University of Valencia)\r\nfor help with the statistical analysis;
Jurgen Kleine-Vehn, Elke Barbez, and\r\nEva Benkova for helpful discussions; the
Salk Institute Genomic Analysis\r\nLaboratory for providing the sequence-indexed
Arabidopsis T-DNA in-\r\nsertion mutants; and the greenhouse section and the microscopy
section\r\nof SCSIE (University of Valencia) and Pilar Selvi for excellent technical\r\nassistance."
author:
- first_name: Gloria
full_name: Sancho Andrés, Gloria
last_name: Sancho Andrés
- first_name: Esther
full_name: Soriano Ortega, Esther
last_name: Soriano Ortega
- first_name: Caiji
full_name: Gao, Caiji
last_name: Gao
- first_name: Joan
full_name: Bernabé Orts, Joan
last_name: Bernabé Orts
- first_name: Madhumitha
full_name: Narasimhan, Madhumitha
id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
last_name: Narasimhan
orcid: 0000-0002-8600-0671
- first_name: Anna
full_name: Müller, Anna
id: 420AB15A-F248-11E8-B48F-1D18A9856A87
last_name: Müller
- first_name: Ricardo
full_name: Tejos, Ricardo
last_name: Tejos
- first_name: Liwen
full_name: Jiang, Liwen
last_name: Jiang
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Fernando
full_name: Aniento, Fernando
last_name: Aniento
- first_name: Maria
full_name: Marcote, Maria
last_name: Marcote
citation:
ama: Sancho Andrés G, Soriano Ortega E, Gao C, et al. Sorting motifs involved in
the trafficking and localization of the PIN1 auxin efflux carrier. Plant Physiology.
2016;171(3):1965-1982. doi:10.1104/pp.16.00373
apa: Sancho Andrés, G., Soriano Ortega, E., Gao, C., Bernabé Orts, J., Narasimhan,
M., Müller, A., … Marcote, M. (2016). Sorting motifs involved in the trafficking
and localization of the PIN1 auxin efflux carrier. Plant Physiology. American
Society of Plant Biologists. https://doi.org/10.1104/pp.16.00373
chicago: Sancho Andrés, Gloria, Esther Soriano Ortega, Caiji Gao, Joan Bernabé Orts,
Madhumitha Narasimhan, Anna Müller, Ricardo Tejos, et al. “Sorting Motifs Involved
in the Trafficking and Localization of the PIN1 Auxin Efflux Carrier.” Plant
Physiology. American Society of Plant Biologists, 2016. https://doi.org/10.1104/pp.16.00373.
ieee: G. Sancho Andrés et al., “Sorting motifs involved in the trafficking
and localization of the PIN1 auxin efflux carrier,” Plant Physiology, vol.
171, no. 3. American Society of Plant Biologists, pp. 1965–1982, 2016.
ista: Sancho Andrés G, Soriano Ortega E, Gao C, Bernabé Orts J, Narasimhan M, Müller
A, Tejos R, Jiang L, Friml J, Aniento F, Marcote M. 2016. Sorting motifs involved
in the trafficking and localization of the PIN1 auxin efflux carrier. Plant Physiology.
171(3), 1965–1982.
mla: Sancho Andrés, Gloria, et al. “Sorting Motifs Involved in the Trafficking and
Localization of the PIN1 Auxin Efflux Carrier.” Plant Physiology, vol.
171, no. 3, American Society of Plant Biologists, 2016, pp. 1965–82, doi:10.1104/pp.16.00373.
short: G. Sancho Andrés, E. Soriano Ortega, C. Gao, J. Bernabé Orts, M. Narasimhan,
A. Müller, R. Tejos, L. Jiang, J. Friml, F. Aniento, M. Marcote, Plant Physiology
171 (2016) 1965–1982.
date_created: 2018-12-11T11:51:01Z
date_published: 2016-07-01T00:00:00Z
date_updated: 2021-01-12T06:49:29Z
day: '01'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1104/pp.16.00373
ec_funded: 1
intvolume: ' 171'
issue: '3'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4936568/
month: '07'
oa: 1
oa_version: Submitted Version
page: 1965 - 1982
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Plant Physiology
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '6059'
quality_controlled: '1'
scopus_import: 1
status: public
title: Sorting motifs involved in the trafficking and localization of the PIN1 auxin
efflux carrier
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 171
year: '2016'
...