---
_id: '14709'
abstract:
- lang: eng
text: Amid the delays due to the global pandemic, in early October 2022, the auxin
community gathered in the idyllic peninsula of Cavtat, Croatia. More than 170
scientists from across the world converged to discuss the latest advancements
in fundamental and applied research in the field. The topics, from signalling
and transport to plant architecture and response to the environment, show how
auxin research must bridge from the molecular realm to macroscopic developmental
responses. This is mirrored in this collection of reviews, contributed by participants
of the Auxin 2022 meeting.
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Marta
full_name: Del Bianco, Marta
last_name: Del Bianco
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Lucia
full_name: Strader, Lucia
last_name: Strader
- first_name: Stefan
full_name: Kepinski, Stefan
last_name: Kepinski
citation:
ama: 'Del Bianco M, Friml J, Strader L, Kepinski S. Auxin research: Creating tools
for a greener future. Journal of Experimental Botany. 2023;74(22):6889-6892.
doi:10.1093/jxb/erad420'
apa: 'Del Bianco, M., Friml, J., Strader, L., & Kepinski, S. (2023). Auxin research:
Creating tools for a greener future. Journal of Experimental Botany. Oxford
University Press. https://doi.org/10.1093/jxb/erad420'
chicago: 'Del Bianco, Marta, Jiří Friml, Lucia Strader, and Stefan Kepinski. “Auxin
Research: Creating Tools for a Greener Future.” Journal of Experimental Botany.
Oxford University Press, 2023. https://doi.org/10.1093/jxb/erad420.'
ieee: 'M. Del Bianco, J. Friml, L. Strader, and S. Kepinski, “Auxin research: Creating
tools for a greener future,” Journal of Experimental Botany, vol. 74, no.
22. Oxford University Press, pp. 6889–6892, 2023.'
ista: 'Del Bianco M, Friml J, Strader L, Kepinski S. 2023. Auxin research: Creating
tools for a greener future. Journal of Experimental Botany. 74(22), 6889–6892.'
mla: 'Del Bianco, Marta, et al. “Auxin Research: Creating Tools for a Greener Future.”
Journal of Experimental Botany, vol. 74, no. 22, Oxford University Press,
2023, pp. 6889–92, doi:10.1093/jxb/erad420.'
short: M. Del Bianco, J. Friml, L. Strader, S. Kepinski, Journal of Experimental
Botany 74 (2023) 6889–6892.
date_created: 2023-12-24T23:00:53Z
date_published: 2023-12-01T00:00:00Z
date_updated: 2024-01-02T09:29:24Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1093/jxb/erad420
external_id:
pmid:
- '38038239'
file:
- access_level: open_access
checksum: f66fb960fd791dea53fd0e087f2fbbe8
content_type: application/pdf
creator: dernst
date_created: 2024-01-02T09:23:57Z
date_updated: 2024-01-02T09:23:57Z
file_id: '14724'
file_name: 2023_JourExperimentalBotany_DelBianco.pdf
file_size: 425194
relation: main_file
success: 1
file_date_updated: 2024-01-02T09:23:57Z
has_accepted_license: '1'
intvolume: ' 74'
issue: '22'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
page: 6889-6892
pmid: 1
publication: Journal of Experimental Botany
publication_identifier:
eissn:
- 1460-2431
issn:
- 0022-0957
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Auxin research: Creating tools for a greener future'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 74
year: '2023'
...
---
_id: '14776'
abstract:
- lang: eng
text: Soluble chaperones residing in the endoplasmic reticulum (ER) play vitally
important roles in folding and quality control of newly synthesized proteins that
transiently pass through the ER en route to their final destinations. These soluble
residents of the ER are themselves endowed with an ER retrieval signal that enables
the cell to bring the escaped residents back from the Golgi. Here, by using purified
proteins, we showed that Nicotiana tabacum phytaspase, a plant aspartate-specific
protease, introduces two breaks at the C-terminus of the N. tabacum ER resident
calreticulin-3. These cleavages resulted in removal of either a dipeptide or a
hexapeptide from the C-terminus of calreticulin-3 encompassing part or all of
the ER retrieval signal. Consistently, expression of the calreticulin-3 derivative
mimicking the phytaspase cleavage product in Nicotiana benthamiana cells demonstrated
loss of the ER accumulation of the protein. Notably, upon its escape from the
ER, calreticulin-3 was further processed by an unknown protease(s) to generate
the free N-terminal (N) domain of calreticulin-3, which was ultimately secreted
into the apoplast. Our study thus identified a specific proteolytic enzyme capable
of precise detachment of the ER retrieval signal from a plant ER resident protein,
with implications for the further fate of the escaped resident.
acknowledgement: "We thank C.U.T. Hellen for critically reading the manuscript. The
MALDI MS facility and CLSM became available to us in the framework of Moscow State
University Development Programs PNG 5.13 and PNR 5.13.\r\nThis work was funded by
the Russian Science Foundation, grant numbers 19-14-00010 and 22-14-00071."
article_number: '16527'
article_processing_charge: Yes
article_type: original
author:
- first_name: Anastasiia
full_name: Teplova, Anastasiia
id: e3736151-106c-11ec-b916-c2558e2762c6
last_name: Teplova
- first_name: Artemii A.
full_name: Pigidanov, Artemii A.
last_name: Pigidanov
- first_name: Marina V.
full_name: Serebryakova, Marina V.
last_name: Serebryakova
- first_name: Sergei A.
full_name: Golyshev, Sergei A.
last_name: Golyshev
- first_name: Raisa A.
full_name: Galiullina, Raisa A.
last_name: Galiullina
- first_name: Nina V.
full_name: Chichkova, Nina V.
last_name: Chichkova
- first_name: Andrey B.
full_name: Vartapetian, Andrey B.
last_name: Vartapetian
citation:
ama: Teplova A, Pigidanov AA, Serebryakova MV, et al. Phytaspase Is capable of detaching
the endoplasmic reticulum retrieval signal from tobacco calreticulin-3. International
Journal of Molecular Sciences. 2023;24(22). doi:10.3390/ijms242216527
apa: Teplova, A., Pigidanov, A. A., Serebryakova, M. V., Golyshev, S. A., Galiullina,
R. A., Chichkova, N. V., & Vartapetian, A. B. (2023). Phytaspase Is capable
of detaching the endoplasmic reticulum retrieval signal from tobacco calreticulin-3.
International Journal of Molecular Sciences. MDPI. https://doi.org/10.3390/ijms242216527
chicago: Teplova, Anastasiia, Artemii A. Pigidanov, Marina V. Serebryakova, Sergei
A. Golyshev, Raisa A. Galiullina, Nina V. Chichkova, and Andrey B. Vartapetian.
“Phytaspase Is Capable of Detaching the Endoplasmic Reticulum Retrieval Signal
from Tobacco Calreticulin-3.” International Journal of Molecular Sciences.
MDPI, 2023. https://doi.org/10.3390/ijms242216527.
ieee: A. Teplova et al., “Phytaspase Is capable of detaching the endoplasmic
reticulum retrieval signal from tobacco calreticulin-3,” International Journal
of Molecular Sciences, vol. 24, no. 22. MDPI, 2023.
ista: Teplova A, Pigidanov AA, Serebryakova MV, Golyshev SA, Galiullina RA, Chichkova
NV, Vartapetian AB. 2023. Phytaspase Is capable of detaching the endoplasmic reticulum
retrieval signal from tobacco calreticulin-3. International Journal of Molecular
Sciences. 24(22), 16527.
mla: Teplova, Anastasiia, et al. “Phytaspase Is Capable of Detaching the Endoplasmic
Reticulum Retrieval Signal from Tobacco Calreticulin-3.” International Journal
of Molecular Sciences, vol. 24, no. 22, 16527, MDPI, 2023, doi:10.3390/ijms242216527.
short: A. Teplova, A.A. Pigidanov, M.V. Serebryakova, S.A. Golyshev, R.A. Galiullina,
N.V. Chichkova, A.B. Vartapetian, International Journal of Molecular Sciences
24 (2023).
date_created: 2024-01-10T09:24:35Z
date_published: 2023-11-01T00:00:00Z
date_updated: 2024-01-10T13:41:10Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.3390/ijms242216527
external_id:
isi:
- '001113792600001'
pmid:
- '38003717'
file:
- access_level: open_access
checksum: 4df7d206ba022b7f54eff1f0aec1659a
content_type: application/pdf
creator: dernst
date_created: 2024-01-10T13:39:42Z
date_updated: 2024-01-10T13:39:42Z
file_id: '14791'
file_name: 2023_IJMS_Teplova.pdf
file_size: 2637784
relation: main_file
success: 1
file_date_updated: 2024-01-10T13:39:42Z
has_accepted_license: '1'
intvolume: ' 24'
isi: 1
issue: '22'
keyword:
- Inorganic Chemistry
- Organic Chemistry
- Physical and Theoretical Chemistry
- Computer Science Applications
- Spectroscopy
- Molecular Biology
- General Medicine
- Catalysis
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
publication: International Journal of Molecular Sciences
publication_identifier:
issn:
- 1422-0067
publication_status: published
publisher: MDPI
quality_controlled: '1'
status: public
title: Phytaspase Is capable of detaching the endoplasmic reticulum retrieval signal
from tobacco calreticulin-3
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 24
year: '2023'
...
---
_id: '13212'
abstract:
- lang: eng
text: Auxin is the major plant hormone regulating growth and development (Friml,
2022). Forward genetic approaches in the model plant Arabidopsis thaliana have
identified major components of auxin signalling and established the canonical
mechanism mediating transcriptional and thus developmental reprogramming. In this
textbook view, TRANSPORT INHIBITOR RESPONSE 1 (TIR1)/AUXIN-SIGNALING F-BOX (AFBs)
are auxin receptors, which act as F-box subunits determining the substrate specificity
of the Skp1-Cullin1-F box protein (SCF) type E3 ubiquitin ligase complex. Auxin
acts as a “molecular glue” increasing the affinity between TIR1/AFBs and the Aux/IAA
repressors. Subsequently, Aux/IAAs are ubiquitinated and degraded, thus releasing
auxin transcription factors from their repression making them free to mediate
transcription of auxin response genes (Yu et al., 2022). Nonetheless, accumulating
evidence suggests existence of rapid, non-transcriptional responses downstream
of TIR1/AFBs such as auxin-induced cytosolic calcium (Ca2+) transients, plasma
membrane depolarization and apoplast alkalinisation, all converging on the process
of root growth inhibition and root gravitropism (Li et al., 2022). Particularly,
these rapid responses are mostly contributed by predominantly cytosolic AFB1,
while the long-term growth responses are mediated by mainly nuclear TIR1 and AFB2-AFB5
(Li et al., 2021; Prigge et al., 2020; Serre et al., 2021). How AFB1 conducts
auxin-triggered rapid responses and how it is different from TIR1 and AFB2-AFB5
remains elusive. Here, we compare the roles of TIR1 and AFB1 in transcriptional
and rapid responses by modulating their subcellular localization in Arabidopsis
and by testing their ability to mediate transcriptional responses when part of
the minimal auxin circuit reconstituted in yeast.
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
acknowledgement: We thank all the authors for sharing the published materials. This
research was supported by the Lab Support Facility and the Imaging and Optics Facility
of ISTA. We thank Lukáš Fiedler (ISTA) for critical reading of the manuscript. This
project was funded by the European Research Council Advanced Grant (ETAP-742985).
article_processing_charge: Yes (via OA deal)
article_type: letter_note
author:
- first_name: Huihuang
full_name: Chen, Huihuang
id: 83c96512-15b2-11ec-abd3-b7eede36184f
last_name: Chen
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Minxia
full_name: Zou, Minxia
id: 5c243f41-03f3-11ec-841c-96faf48a7ef9
last_name: Zou
- first_name: Linlin
full_name: Qi, Linlin
id: 44B04502-A9ED-11E9-B6FC-583AE6697425
last_name: Qi
orcid: 0000-0001-5187-8401
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Chen H, Li L, Zou M, Qi L, Friml J. Distinct functions of TIR1 and AFB1 receptors
in auxin signalling. Molecular Plant. 2023;16(7):1117-1119. doi:10.1016/j.molp.2023.06.007
apa: Chen, H., Li, L., Zou, M., Qi, L., & Friml, J. (2023). Distinct functions
of TIR1 and AFB1 receptors in auxin signalling. Molecular Plant. Elsevier
. https://doi.org/10.1016/j.molp.2023.06.007
chicago: Chen, Huihuang, Lanxin Li, Minxia Zou, Linlin Qi, and Jiří Friml. “Distinct
Functions of TIR1 and AFB1 Receptors in Auxin Signalling.” Molecular Plant.
Elsevier , 2023. https://doi.org/10.1016/j.molp.2023.06.007.
ieee: H. Chen, L. Li, M. Zou, L. Qi, and J. Friml, “Distinct functions of TIR1 and
AFB1 receptors in auxin signalling.,” Molecular Plant, vol. 16, no. 7.
Elsevier , pp. 1117–1119, 2023.
ista: Chen H, Li L, Zou M, Qi L, Friml J. 2023. Distinct functions of TIR1 and AFB1
receptors in auxin signalling. Molecular Plant. 16(7), 1117–1119.
mla: Chen, Huihuang, et al. “Distinct Functions of TIR1 and AFB1 Receptors in Auxin
Signalling.” Molecular Plant, vol. 16, no. 7, Elsevier , 2023, pp. 1117–19,
doi:10.1016/j.molp.2023.06.007.
short: H. Chen, L. Li, M. Zou, L. Qi, J. Friml, Molecular Plant 16 (2023) 1117–1119.
date_created: 2023-07-12T07:32:46Z
date_published: 2023-07-01T00:00:00Z
date_updated: 2024-01-29T10:38:57Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.molp.2023.06.007
ec_funded: 1
external_id:
isi:
- '001044410900001'
pmid:
- '37393433'
file:
- access_level: open_access
checksum: 6012b7e4a2f680ee6c1f84001e2b945f
content_type: application/pdf
creator: dernst
date_created: 2024-01-29T10:37:05Z
date_updated: 2024-01-29T10:37:05Z
file_id: '14894'
file_name: 2023_MolecularPlant_Chen.pdf
file_size: 1000871
relation: main_file
success: 1
file_date_updated: 2024-01-29T10:37:05Z
has_accepted_license: '1'
intvolume: ' 16'
isi: 1
issue: '7'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '07'
oa: 1
oa_version: Published Version
page: 1117-1119
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Molecular Plant
publication_identifier:
eissn:
- 1674-2052
issn:
- 1752-9867
publication_status: published
publisher: 'Elsevier '
quality_controlled: '1'
scopus_import: '1'
status: public
title: Distinct functions of TIR1 and AFB1 receptors in auxin signalling.
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 16
year: '2023'
...
---
_id: '13266'
abstract:
- lang: eng
text: The 3′,5′-cyclic adenosine monophosphate (cAMP) is a versatile second messenger
in many mammalian signaling pathways. However, its role in plants remains not
well-recognized. Recent discovery of adenylate cyclase (AC) activity for transport
inhibitor response 1/auxin-signaling F-box proteins (TIR1/AFB) auxin receptors
and the demonstration of its importance for canonical auxin signaling put plant
cAMP research back into spotlight. This insight briefly summarizes the well-established
cAMP signaling pathways in mammalian cells and describes the turbulent and controversial
history of plant cAMP research highlighting the major progress and the unresolved
points. We also briefly review the current paradigm of auxin signaling to provide
a background for the discussion on the AC activity of TIR1/AFB auxin receptors
and its potential role in transcriptional auxin signaling as well as impact of
these discoveries on plant cAMP research in general.
acknowledgement: 'We gratefully acknowledge our brave colleagues, whose excellent
efforts kept the plant cAMP research going in the last two decades. The authors
were financially supported by the Austrian Science Fund (FWF): I 6123 and P 37051-B.'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Linlin
full_name: Qi, Linlin
id: 44B04502-A9ED-11E9-B6FC-583AE6697425
last_name: Qi
orcid: 0000-0001-5187-8401
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Qi L, Friml J. Tale of cAMP as a second messenger in auxin signaling and beyond.
New Phytologist. 2023;240(2):489-495. doi:10.1111/nph.19123
apa: Qi, L., & Friml, J. (2023). Tale of cAMP as a second messenger in auxin
signaling and beyond. New Phytologist. Wiley. https://doi.org/10.1111/nph.19123
chicago: Qi, Linlin, and Jiří Friml. “Tale of CAMP as a Second Messenger in Auxin
Signaling and Beyond.” New Phytologist. Wiley, 2023. https://doi.org/10.1111/nph.19123.
ieee: L. Qi and J. Friml, “Tale of cAMP as a second messenger in auxin signaling
and beyond,” New Phytologist, vol. 240, no. 2. Wiley, pp. 489–495, 2023.
ista: Qi L, Friml J. 2023. Tale of cAMP as a second messenger in auxin signaling
and beyond. New Phytologist. 240(2), 489–495.
mla: Qi, Linlin, and Jiří Friml. “Tale of CAMP as a Second Messenger in Auxin Signaling
and Beyond.” New Phytologist, vol. 240, no. 2, Wiley, 2023, pp. 489–95,
doi:10.1111/nph.19123.
short: L. Qi, J. Friml, New Phytologist 240 (2023) 489–495.
date_created: 2023-07-23T22:01:13Z
date_published: 2023-10-01T00:00:00Z
date_updated: 2024-01-29T11:21:55Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/nph.19123
external_id:
isi:
- '001026321500001'
pmid:
- '37434303'
file:
- access_level: open_access
checksum: 6d9bbd45b8e7bb3ceee2586d447bacb2
content_type: application/pdf
creator: dernst
date_created: 2024-01-29T11:21:43Z
date_updated: 2024-01-29T11:21:43Z
file_id: '14898'
file_name: 2023_NewPhytologist_Qi.pdf
file_size: 974464
relation: main_file
success: 1
file_date_updated: 2024-01-29T11:21:43Z
has_accepted_license: '1'
intvolume: ' 240'
isi: 1
issue: '2'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
page: 489-495
pmid: 1
project:
- _id: bd76d395-d553-11ed-ba76-f678c14f9033
grant_number: I06123
name: Peptide receptor complexes for auxin canalization and regeneration in Arabidopsis
- _id: 7bcece63-9f16-11ee-852c-ae94e099eeb6
grant_number: P37051
name: Guanylate cyclase activity of TIR1/AFBs auxin receptors
publication: New Phytologist
publication_identifier:
eissn:
- 1469-8137
issn:
- 0028-646X
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Tale of cAMP as a second messenger in auxin signaling and beyond
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 240
year: '2023'
...
---
_id: '13209'
abstract:
- lang: eng
text: The phytohormone auxin plays central roles in many growth and developmental
processes in plants. Development of chemical tools targeting the auxin pathway
is useful for both plant biology and agriculture. Here we reveal that naproxen,
a synthetic compound with anti-inflammatory activity in humans, acts as an auxin
transport inhibitor targeting PIN-FORMED (PIN) transporters in plants. Physiological
experiments indicate that exogenous naproxen treatment affects pleiotropic auxin-regulated
developmental processes. Additional cellular and biochemical evidence indicates
that naproxen suppresses auxin transport, specifically PIN-mediated auxin efflux.
Moreover, biochemical and structural analyses confirm that naproxen binds directly
to PIN1 protein via the same binding cavity as the indole-3-acetic acid substrate.
Thus, by combining cellular, biochemical, and structural approaches, this study
clearly establishes that naproxen is a PIN inhibitor and elucidates the underlying
mechanisms. Further use of this compound may advance our understanding of the
molecular mechanisms of PIN-mediated auxin transport and expand our toolkit in
auxin biology and agriculture.
acknowledgement: "This work was supported by the Strategic Priority Research Program
of the Chinese Academy of Sciences (XDB37020103 to Linfeng Sun); research funds
from the Center for Advanced Interdisciplinary Science\r\nand Biomedicine of IHM,
Division of Life Sciences and Medicine, University of Science and Technology of
China (QYPY20220012 to S.T.); start-up funding from the University of Science and
Technology of China and the\r\nChinese Academy of Sciences (GG9100007007, KY9100000026,KY9100000051,
and KJ2070000079 to S.T.); the National Natural Science Foundation of China (31900885
to X.L. and 31870732 to Linfeng Sun); the Natural Science Foundation of Anhui Province
(2008085MC90 to X.L. and 2008085J15 to Linfeng Sun); the Fundamental Research Funds
for the Central Universities (WK9100000021 to S.T. and WK9100000031 to Linfeng Sun);
and the USTC Research Funds of the Double First-Class Initiative (YD9100002016 to
S.T. and YD9100002004 to Linfeng Sun). Linfeng Sun is supported by an Outstanding
Young Scholar Award from the Qiu Shi Science and Technologies Foundation and a Young
Scholar Award from the Cyrus Tang Foundation.We thank Dr. Yang Zhao for sharing
published materials (Center for Excellence in Molecular Plant Sciences, Chinese
Academy of Sciences) and the Cryo-EM Center of the University of Science and Technology
of China for the EM facility support. We are grateful to Y. Gao and all other staff
members for their technical support on cryo-EM data collection. "
article_number: '100632'
article_processing_charge: Yes
article_type: original
author:
- first_name: Jing
full_name: Xia, Jing
last_name: Xia
- first_name: Mengjuan
full_name: Kong, Mengjuan
last_name: Kong
- first_name: Zhisen
full_name: Yang, Zhisen
last_name: Yang
- first_name: Lianghanxiao
full_name: Sun, Lianghanxiao
last_name: Sun
- first_name: Yakun
full_name: Peng, Yakun
last_name: Peng
- first_name: Yanbo
full_name: Mao, Yanbo
last_name: Mao
- first_name: Hong
full_name: Wei, Hong
last_name: Wei
- first_name: Wei
full_name: Ying, Wei
last_name: Ying
- first_name: Yongxiao
full_name: Gao, Yongxiao
last_name: Gao
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Jianping
full_name: Weng, Jianping
last_name: Weng
- first_name: Xin
full_name: Liu, Xin
last_name: Liu
- first_name: Linfeng
full_name: Sun, Linfeng
last_name: Sun
- first_name: Shutang
full_name: Tan, Shutang
last_name: Tan
citation:
ama: Xia J, Kong M, Yang Z, et al. Chemical inhibition of Arabidopsis PIN-FORMED
auxin transporters by the anti-inflammatory drug naproxen. Plant Communications.
2023;4(6). doi:10.1016/j.xplc.2023.100632
apa: Xia, J., Kong, M., Yang, Z., Sun, L., Peng, Y., Mao, Y., … Tan, S. (2023).
Chemical inhibition of Arabidopsis PIN-FORMED auxin transporters by the anti-inflammatory
drug naproxen. Plant Communications. Elsevier . https://doi.org/10.1016/j.xplc.2023.100632
chicago: Xia, Jing, Mengjuan Kong, Zhisen Yang, Lianghanxiao Sun, Yakun Peng, Yanbo
Mao, Hong Wei, et al. “Chemical Inhibition of Arabidopsis PIN-FORMED Auxin Transporters
by the Anti-Inflammatory Drug Naproxen.” Plant Communications. Elsevier
, 2023. https://doi.org/10.1016/j.xplc.2023.100632.
ieee: J. Xia et al., “Chemical inhibition of Arabidopsis PIN-FORMED auxin
transporters by the anti-inflammatory drug naproxen,” Plant Communications,
vol. 4, no. 6. Elsevier , 2023.
ista: Xia J, Kong M, Yang Z, Sun L, Peng Y, Mao Y, Wei H, Ying W, Gao Y, Friml J,
Weng J, Liu X, Sun L, Tan S. 2023. Chemical inhibition of Arabidopsis PIN-FORMED
auxin transporters by the anti-inflammatory drug naproxen. Plant Communications.
4(6), 100632.
mla: Xia, Jing, et al. “Chemical Inhibition of Arabidopsis PIN-FORMED Auxin Transporters
by the Anti-Inflammatory Drug Naproxen.” Plant Communications, vol. 4,
no. 6, 100632, Elsevier , 2023, doi:10.1016/j.xplc.2023.100632.
short: J. Xia, M. Kong, Z. Yang, L. Sun, Y. Peng, Y. Mao, H. Wei, W. Ying, Y. Gao,
J. Friml, J. Weng, X. Liu, L. Sun, S. Tan, Plant Communications 4 (2023).
date_created: 2023-07-12T07:32:00Z
date_published: 2023-11-13T00:00:00Z
date_updated: 2024-01-30T10:55:34Z
day: '13'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.xplc.2023.100632
external_id:
isi:
- '001113003000001'
pmid:
- '37254481'
file:
- access_level: open_access
checksum: f8ef92af6096834f91ce38587fb1db9f
content_type: application/pdf
creator: dernst
date_created: 2024-01-30T10:54:40Z
date_updated: 2024-01-30T10:54:40Z
file_id: '14900'
file_name: 2023_PlantCommunications_Xia.pdf
file_size: 1434862
relation: main_file
success: 1
file_date_updated: 2024-01-30T10:54:40Z
has_accepted_license: '1'
intvolume: ' 4'
isi: 1
issue: '6'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
publication: Plant Communications
publication_identifier:
eissn:
- 2590-3462
publication_status: published
publisher: 'Elsevier '
quality_controlled: '1'
status: public
title: Chemical inhibition of Arabidopsis PIN-FORMED auxin transporters by the anti-inflammatory
drug naproxen
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 4
year: '2023'
...
---
_id: '13201'
abstract:
- lang: eng
text: As a crucial nitrogen source, nitrate (NO3−) is a key nutrient for plants.
Accordingly, root systems adapt to maximize NO3− availability, a developmental
regulation also involving the phytohormone auxin. Nonetheless, the molecular mechanisms
underlying this regulation remain poorly understood. Here, we identify low-nitrate-resistant
mutant (lonr) in Arabidopsis (Arabidopsis thaliana), whose root growth fails to
adapt to low-NO3− conditions. lonr2 is defective in the high-affinity NO3− transporter
NRT2.1. lonr2 (nrt2.1) mutants exhibit defects in polar auxin transport, and their
low-NO3−-induced root phenotype depends on the PIN7 auxin exporter activity. NRT2.1
directly associates with PIN7 and antagonizes PIN7-mediated auxin efflux depending
on NO3− levels. These results reveal a mechanism by which NRT2.1 in response to
NO3− limitation directly regulates auxin transport activity and, thus, root growth.
This adaptive mechanism contributes to the root developmental plasticity to help
plants cope with changes in NO3− availability.
acknowledgement: We are grateful to Caifu Jiang for providing ethyl metha-nesulfonate-
mutagenized population, Yi Wang for providing Xenopus oocytes, Jun Fan and Zhaosheng
Kong for providing tobacco BY- 2 cells, and Claus Schwechheimer, Alain Gojon, and
Shutang Tan for helpful discussions. This work was supported by the National Key
Research and Development Program of China (2021YFF1000500), the National Natural Science Foundation of China (32170265 and 32022007), Hainan Provincial Natural Science Foundation of China (323CXTD379), Chinese Universities Scientific Fund (2023TC019), Beijing Municipal Natural Science Foundation (5192011), Beijing Outstanding University Discipline Program, and China
Postdoctoral Science Foundation (BH2020259460).
article_number: e2221313120
article_processing_charge: No
article_type: original
author:
- first_name: Yalu
full_name: Wang, Yalu
last_name: Wang
- first_name: Zhi
full_name: Yuan, Zhi
last_name: Yuan
- first_name: Jinyi
full_name: Wang, Jinyi
last_name: Wang
- first_name: Huixin
full_name: Xiao, Huixin
last_name: Xiao
- first_name: Lu
full_name: Wan, Lu
last_name: Wan
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Yan
full_name: Guo, Yan
last_name: Guo
- first_name: Zhizhong
full_name: Gong, Zhizhong
last_name: Gong
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Jing
full_name: Zhang, Jing
last_name: Zhang
citation:
ama: Wang Y, Yuan Z, Wang J, et al. The nitrate transporter NRT2.1 directly antagonizes
PIN7-mediated auxin transport for root growth adaptation. Proceedings of the
National Academy of Sciences of the United States of America. 2023;120(25).
doi:10.1073/pnas.2221313120
apa: Wang, Y., Yuan, Z., Wang, J., Xiao, H., Wan, L., Li, L., … Zhang, J. (2023).
The nitrate transporter NRT2.1 directly antagonizes PIN7-mediated auxin transport
for root growth adaptation. Proceedings of the National Academy of Sciences
of the United States of America. National Academy of Sciences. https://doi.org/10.1073/pnas.2221313120
chicago: Wang, Yalu, Zhi Yuan, Jinyi Wang, Huixin Xiao, Lu Wan, Lanxin Li, Yan Guo,
Zhizhong Gong, Jiří Friml, and Jing Zhang. “The Nitrate Transporter NRT2.1 Directly
Antagonizes PIN7-Mediated Auxin Transport for Root Growth Adaptation.” Proceedings
of the National Academy of Sciences of the United States of America. National
Academy of Sciences, 2023. https://doi.org/10.1073/pnas.2221313120.
ieee: Y. Wang et al., “The nitrate transporter NRT2.1 directly antagonizes
PIN7-mediated auxin transport for root growth adaptation,” Proceedings of the
National Academy of Sciences of the United States of America, vol. 120, no.
25. National Academy of Sciences, 2023.
ista: Wang Y, Yuan Z, Wang J, Xiao H, Wan L, Li L, Guo Y, Gong Z, Friml J, Zhang
J. 2023. The nitrate transporter NRT2.1 directly antagonizes PIN7-mediated auxin
transport for root growth adaptation. Proceedings of the National Academy of Sciences
of the United States of America. 120(25), e2221313120.
mla: Wang, Yalu, et al. “The Nitrate Transporter NRT2.1 Directly Antagonizes PIN7-Mediated
Auxin Transport for Root Growth Adaptation.” Proceedings of the National Academy
of Sciences of the United States of America, vol. 120, no. 25, e2221313120,
National Academy of Sciences, 2023, doi:10.1073/pnas.2221313120.
short: Y. Wang, Z. Yuan, J. Wang, H. Xiao, L. Wan, L. Li, Y. Guo, Z. Gong, J. Friml,
J. Zhang, Proceedings of the National Academy of Sciences of the United States
of America 120 (2023).
date_created: 2023-07-09T22:01:12Z
date_published: 2023-06-12T00:00:00Z
date_updated: 2023-12-13T23:30:04Z
day: '12'
ddc:
- '570'
department:
- _id: JiFr
doi: 10.1073/pnas.2221313120
external_id:
isi:
- '001030689600003'
pmid:
- '37307446'
file:
- access_level: open_access
checksum: d800e06252eaefba28531fa9440f23f0
content_type: application/pdf
creator: alisjak
date_created: 2023-07-10T08:48:40Z
date_updated: 2023-12-13T23:30:03Z
embargo: 2023-12-12
file_id: '13204'
file_name: 2023_PNAS_Wang.pdf
file_size: 5244581
relation: main_file
file_date_updated: 2023-12-13T23:30:03Z
has_accepted_license: '1'
intvolume: ' 120'
isi: 1
issue: '25'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
pmid: 1
publication: Proceedings of the National Academy of Sciences of the United States
of America
publication_identifier:
eissn:
- 1091-6490
issn:
- 0027-8424
publication_status: published
publisher: National Academy of Sciences
quality_controlled: '1'
scopus_import: '1'
status: public
title: The nitrate transporter NRT2.1 directly antagonizes PIN7-mediated auxin transport
for root growth adaptation
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 120
year: '2023'
...
---
_id: '14510'
acknowledged_ssus:
- _id: EM-Fac
- _id: Bio
- _id: LifeSc
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Nataliia
full_name: Gnyliukh, Nataliia
id: 390C1120-F248-11E8-B48F-1D18A9856A87
last_name: Gnyliukh
orcid: 0000-0002-2198-0509
citation:
ama: Gnyliukh N. Mechanism of clathrin-coated vesicle formation during endocytosis
in plants. 2023. doi:10.15479/at:ista:14510
apa: Gnyliukh, N. (2023). Mechanism of clathrin-coated vesicle formation during
endocytosis in plants. Institute of Science and Technology Austria. https://doi.org/10.15479/at:ista:14510
chicago: Gnyliukh, Nataliia. “Mechanism of Clathrin-Coated Vesicle Formation during
Endocytosis in Plants.” Institute of Science and Technology Austria, 2023. https://doi.org/10.15479/at:ista:14510.
ieee: N. Gnyliukh, “Mechanism of clathrin-coated vesicle formation during endocytosis
in plants,” Institute of Science and Technology Austria, 2023.
ista: Gnyliukh N. 2023. Mechanism of clathrin-coated vesicle formation during endocytosis
in plants. Institute of Science and Technology Austria.
mla: Gnyliukh, Nataliia. Mechanism of Clathrin-Coated Vesicle Formation during
Endocytosis in Plants. Institute of Science and Technology Austria, 2023,
doi:10.15479/at:ista:14510.
short: N. Gnyliukh, Mechanism of Clathrin-Coated Vesicle Formation during Endocytosis
in Plants, Institute of Science and Technology Austria, 2023.
date_created: 2023-11-10T09:10:06Z
date_published: 2023-11-10T00:00:00Z
date_updated: 2024-03-27T23:30:45Z
day: '10'
ddc:
- '570'
degree_awarded: PhD
department:
- _id: GradSch
- _id: JiFr
- _id: MaLo
doi: 10.15479/at:ista:14510
ec_funded: 1
file:
- access_level: closed
checksum: 3d5e680bfc61f98e308c434f45cc9bd6
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: ngnyliuk
date_created: 2023-11-20T09:18:51Z
date_updated: 2023-11-20T09:18:51Z
file_id: '14567'
file_name: Thesis_Gnyliukh_final_08_11_23.docx
file_size: 20824903
relation: source_file
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checksum: bfc96d47fc4e7e857dd71656097214a4
content_type: application/pdf
creator: ngnyliuk
date_created: 2023-11-20T09:23:11Z
date_updated: 2023-11-23T13:10:55Z
embargo: 2024-11-23
embargo_to: open_access
file_id: '14568'
file_name: Thesis_Gnyliukh_final_20_11_23.pdf
file_size: 24871844
relation: main_file
file_date_updated: 2023-11-23T13:10:55Z
has_accepted_license: '1'
keyword:
- Clathrin-Mediated Endocytosis
- vesicle scission
- Dynamin-Related Protein 2
- SH3P2
- TPLATE complex
- Total internal reflection fluorescence microscopy
- Arabidopsis thaliana
language:
- iso: eng
month: '11'
oa_version: Published Version
page: '180'
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
publication_identifier:
isbn:
- 978-3-99078-037-4
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '14591'
relation: part_of_dissertation
status: public
- id: '9887'
relation: part_of_dissertation
status: public
- id: '8139'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Martin
full_name: Loose, Martin
id: 462D4284-F248-11E8-B48F-1D18A9856A87
last_name: Loose
orcid: 0000-0001-7309-9724
title: Mechanism of clathrin-coated vesicle formation during endocytosis in plants
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: dissertation
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
year: '2023'
...
---
_id: '10016'
abstract:
- lang: eng
text: 'Auxin has always been at the forefront of research in plant physiology and
development. Since the earliest contemplations by Julius von Sachs and Charles
Darwin, more than a century-long struggle has been waged to understand its function.
This largely reflects the failures, successes, and inevitable progress in the
entire field of plant signaling and development. Here I present 14 stations on
our long and sometimes mystical journey to understand auxin. These highlights
were selected to give a flavor of the field and to show the scope and limits of
our current knowledge. A special focus is put on features that make auxin unique
among phytohormones, such as its dynamic, directional transport network, which
integrates external and internal signals, including self-organizing feedback.
Accented are persistent mysteries and controversies. The unexpected discoveries
related to rapid auxin responses and growth regulation recently disturbed our
contentment regarding understanding of the auxin signaling mechanism. These new
revelations, along with advances in technology, usher us into a new, exciting
era in auxin research. '
acknowledgement: "The author thanks the whole community of researchers consciously
or unconsciously working on questions related to auxin, whose hard work and enthusiasm
contributed to development of this exciting story. Particular thanks go to many\r\nbrilliant
present and past members of the Friml group and our numerous excellent collaborators,
without whom my own personal journey would not be possible. The way of the cross
with its 14 stations is a popular devotion among Roman Catholics and inspires them
to make a spiritual pilgrimage through contemplation of Christ on his last day.
Its aspects of gradual progress, struggle, passion, and revelation served as an
inspiration for the formal depiction of our journey to understanding auxin as described
in this review. It is in no way intended to reflect the personal beliefs of the
author and readers. I am grateful to Nick Barton, Eva Benková, Lenka Caisová, Matyáš
Fendrych, Lukáš Fiedler, Monika Frátriková, Jarmila Frimlová, Michelle Gallei, Jakub
Hajný, Lukas Hoermayer, Alexandra Mally, Ondrˇej Novák, Jan Petrášek, Aleš Pěnčík,
Steffen Vanneste, Tongda Xu, and Zhenbiao Yang for their valuable comments. Special
thanks go to Michelle Gallei for her invaluable assistance with the figures."
article_number: a039859
article_processing_charge: No
article_type: review
author:
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Friml J. Fourteen stations of auxin. Cold Spring Harbor Perspectives in
Biology. 2022;14(5). doi:10.1101/cshperspect.a039859
apa: Friml, J. (2022). Fourteen stations of auxin. Cold Spring Harbor Perspectives
in Biology. Cold Spring Harbor Laboratory. https://doi.org/10.1101/cshperspect.a039859
chicago: Friml, Jiří. “Fourteen Stations of Auxin.” Cold Spring Harbor Perspectives
in Biology. Cold Spring Harbor Laboratory, 2022. https://doi.org/10.1101/cshperspect.a039859 .
ieee: J. Friml, “Fourteen stations of auxin,” Cold Spring Harbor Perspectives
in Biology, vol. 14, no. 5. Cold Spring Harbor Laboratory, 2022.
ista: Friml J. 2022. Fourteen stations of auxin. Cold Spring Harbor Perspectives
in Biology. 14(5), a039859.
mla: Friml, Jiří. “Fourteen Stations of Auxin.” Cold Spring Harbor Perspectives
in Biology, vol. 14, no. 5, a039859, Cold Spring Harbor Laboratory, 2022,
doi:10.1101/cshperspect.a039859
.
short: J. Friml, Cold Spring Harbor Perspectives in Biology 14 (2022).
date_created: 2021-09-14T11:36:53Z
date_published: 2022-05-27T00:00:00Z
date_updated: 2023-08-02T06:54:42Z
day: '27'
department:
- _id: JiFr
doi: '10.1101/cshperspect.a039859 '
external_id:
isi:
- '000806563000003'
pmid:
- '34400554'
intvolume: ' 14'
isi: 1
issue: '5'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: 'https://doi.org/10.1101/cshperspect.a039859 '
month: '05'
oa: 1
oa_version: Published Version
pmid: 1
publication: Cold Spring Harbor Perspectives in Biology
publication_identifier:
issn:
- 1943-0264
publication_status: published
publisher: Cold Spring Harbor Laboratory
quality_controlled: '1'
scopus_import: '1'
status: public
title: Fourteen stations of auxin
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 14
year: '2022'
...
---
_id: '10583'
abstract:
- lang: eng
text: The synthetic strigolactone (SL) analog, rac-GR24, has been instrumental in
studying the role of SLs as well as karrikins because it activates the receptors
DWARF14 (D14) and KARRIKIN INSENSITIVE 2 (KAI2) of their signaling pathways, respectively.
Treatment with rac-GR24 modifies the root architecture at different levels, such
as decreasing the lateral root density (LRD), while promoting root hair elongation
or flavonol accumulation. Previously, we have shown that the flavonol biosynthesis
is transcriptionally activated in the root by rac-GR24 treatment, but, thus far,
the molecular players involved in that response have remained unknown. To get
an in-depth insight into the changes that occur after the compound is perceived
by the roots, we compared the root transcriptomes of the wild type and the more
axillary growth2 (max2) mutant, affected in both SL and karrikin signaling pathways,
with and without rac-GR24 treatment. Quantitative reverse transcription (qRT)-PCR,
reporter line analysis and mutant phenotyping indicated that the flavonol response
and the root hair elongation are controlled by the ELONGATED HYPOCOTYL 5 (HY5)
and MYB12 transcription factors, but HY5, in contrast to MYB12, affects the LRD
as well. Furthermore, we identified the transcription factors TARGET OF MONOPTEROS
5 (TMO5) and TMO5 LIKE1 as negative and the Mediator complex as positive regulators
of the rac-GR24 effect on LRD. Altogether, hereby, we get closer toward understanding
the molecular mechanisms that underlay the rac-GR24 responses in the root.
acknowledgement: The authors thank Ralf Stracke (Bielefeld University, Bielefeld,
Germany) for providing the myb mutants and their colleagues Bert De Rybel for the
tmo5t;mo5l1 double mutant, Boris Parizot for tips on the RNA-seq analysis, Veronique
Storme for statistical help on both the RNA-seq and lateral root density, and Martine
De Cock for help in preparing the manuscript.
article_processing_charge: No
article_type: original
author:
- first_name: Sylwia
full_name: Struk, Sylwia
last_name: Struk
- first_name: Lukas
full_name: Braem, Lukas
last_name: Braem
- first_name: Cedrick
full_name: Matthys, Cedrick
last_name: Matthys
- first_name: Alan
full_name: Walton, Alan
last_name: Walton
- first_name: Nick
full_name: Vangheluwe, Nick
last_name: Vangheluwe
- first_name: Stan
full_name: Van Praet, Stan
last_name: Van Praet
- first_name: Lingxiang
full_name: Jiang, Lingxiang
last_name: Jiang
- first_name: Pawel
full_name: Baster, Pawel
id: 3028BD74-F248-11E8-B48F-1D18A9856A87
last_name: Baster
- first_name: Carolien
full_name: De Cuyper, Carolien
last_name: De Cuyper
- first_name: Francois-Didier
full_name: Boyer, Francois-Didier
last_name: Boyer
- first_name: Elisabeth
full_name: Stes, Elisabeth
last_name: Stes
- first_name: Tom
full_name: Beeckman, Tom
last_name: Beeckman
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Kris
full_name: Gevaert, Kris
last_name: Gevaert
- first_name: Sofie
full_name: Goormachtig, Sofie
last_name: Goormachtig
citation:
ama: Struk S, Braem L, Matthys C, et al. Transcriptional analysis in the Arabidopsis
roots reveals new regulators that link rac-GR24 treatment with changes in flavonol
accumulation, root hair elongation and lateral root density. Plant & Cell
Physiology. 2022;63(1):104-119. doi:10.1093/pcp/pcab149
apa: Struk, S., Braem, L., Matthys, C., Walton, A., Vangheluwe, N., Van Praet, S.,
… Goormachtig, S. (2022). Transcriptional analysis in the Arabidopsis roots reveals
new regulators that link rac-GR24 treatment with changes in flavonol accumulation,
root hair elongation and lateral root density. Plant & Cell Physiology.
Oxford University Press. https://doi.org/10.1093/pcp/pcab149
chicago: Struk, Sylwia, Lukas Braem, Cedrick Matthys, Alan Walton, Nick Vangheluwe,
Stan Van Praet, Lingxiang Jiang, et al. “Transcriptional Analysis in the Arabidopsis
Roots Reveals New Regulators That Link Rac-GR24 Treatment with Changes in Flavonol
Accumulation, Root Hair Elongation and Lateral Root Density.” Plant & Cell
Physiology. Oxford University Press, 2022. https://doi.org/10.1093/pcp/pcab149.
ieee: S. Struk et al., “Transcriptional analysis in the Arabidopsis roots
reveals new regulators that link rac-GR24 treatment with changes in flavonol accumulation,
root hair elongation and lateral root density,” Plant & Cell Physiology,
vol. 63, no. 1. Oxford University Press, pp. 104–119, 2022.
ista: Struk S, Braem L, Matthys C, Walton A, Vangheluwe N, Van Praet S, Jiang L,
Baster P, De Cuyper C, Boyer F-D, Stes E, Beeckman T, Friml J, Gevaert K, Goormachtig
S. 2022. Transcriptional analysis in the Arabidopsis roots reveals new regulators
that link rac-GR24 treatment with changes in flavonol accumulation, root hair
elongation and lateral root density. Plant & Cell Physiology. 63(1), 104–119.
mla: Struk, Sylwia, et al. “Transcriptional Analysis in the Arabidopsis Roots Reveals
New Regulators That Link Rac-GR24 Treatment with Changes in Flavonol Accumulation,
Root Hair Elongation and Lateral Root Density.” Plant & Cell Physiology,
vol. 63, no. 1, Oxford University Press, 2022, pp. 104–19, doi:10.1093/pcp/pcab149.
short: S. Struk, L. Braem, C. Matthys, A. Walton, N. Vangheluwe, S. Van Praet, L.
Jiang, P. Baster, C. De Cuyper, F.-D. Boyer, E. Stes, T. Beeckman, J. Friml, K.
Gevaert, S. Goormachtig, Plant & Cell Physiology 63 (2022) 104–119.
date_created: 2021-12-28T11:44:18Z
date_published: 2022-01-21T00:00:00Z
date_updated: 2023-08-02T13:40:43Z
day: '21'
department:
- _id: JiFr
doi: 10.1093/pcp/pcab149
external_id:
isi:
- '000877899400009'
pmid:
- '34791413'
intvolume: ' 63'
isi: 1
issue: '1'
keyword:
- flavonols
- MAX2
- rac-Gr24
- RNA-seq
- root development
- transcriptional regulation
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1093/pcp/pcab149
month: '01'
oa: 1
oa_version: Published Version
page: 104-119
pmid: 1
publication: Plant & Cell Physiology
publication_identifier:
eissn:
- 1471-9053
issn:
- 0032-0781
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Transcriptional analysis in the Arabidopsis roots reveals new regulators that
link rac-GR24 treatment with changes in flavonol accumulation, root hair elongation
and lateral root density
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 63
year: '2022'
...
---
_id: '10717'
abstract:
- lang: eng
text: Much of what we know about the role of auxin in plant development derives
from exogenous manipulations of auxin distribution and signaling, using inhibitors,
auxins and auxin analogs. In this context, synthetic auxin analogs, such as 1-Naphtalene
Acetic Acid (1-NAA), are often favored over the endogenous auxin indole-3-acetic
acid (IAA), in part due to their higher stability. While such auxin analogs have
proven to be instrumental to reveal the various faces of auxin, they display in
some cases distinct bioactivities compared to IAA. Here, we focused on the effect
of auxin analogs on the accumulation of PIN proteins in Brefeldin A-sensitive
endosomal aggregations (BFA bodies), and the correlation with the ability to elicit
Ca 2+ responses. For a set of commonly used auxin analogs, we evaluated if auxin-analog
induced Ca 2+ signaling inhibits PIN accumulation. Not all auxin analogs elicited
a Ca 2+ response, and their differential ability to elicit Ca 2+ responses correlated
partially with their ability to inhibit BFA-body formation. However, in tir1/afb
and cngc14, 1-NAA-induced Ca 2+ signaling was strongly impaired, yet 1-NAA still
could inhibit PIN accumulation in BFA bodies. This demonstrates that TIR1/AFB-CNGC14-dependent
Ca 2+ signaling does not inhibit BFA body formation in Arabidopsis roots.
acknowledgement: "We thank Joerg Kudla (WWU Munster, Germany), Petra Dietrich (F.A.
University of Erlangen-Nurnberg, Germany) for sharing published materials, and NASC
for providing seeds. We thank Veronique Storme for help with the statistical analyses.
Part of the imaging analysis was carried out at NOLIMITS, an advanced imaging facility
established by the University of Milan.\r\nThis work was supported by grants of
the China Scholarship Council (CSC) to RW and JC; Fonds Wetenschappelijk Onderzoek
(FWO) to TB and (G002220N) SV; the special research fund of Ghent University to
EH; the Deutsche Forschungsgemeinschaft (DFG) through Grants within FOR964 (MK and
KS); Piano di Sviluppo di Ateneo 2019 (University of Milan) to AC; the European
Research Council (ERC) T-Rex project 682436 to DVD; the ERC ETAP project 742985
to JF, and by a PhD fellowship from the University of Milan to MG."
article_number: erac019
article_processing_charge: No
article_type: original
author:
- first_name: R
full_name: Wang, R
last_name: Wang
- first_name: E
full_name: Himschoot, E
last_name: Himschoot
- first_name: M
full_name: Grenzi, M
last_name: Grenzi
- first_name: J
full_name: Chen, J
last_name: Chen
- first_name: A
full_name: Safi, A
last_name: Safi
- first_name: M
full_name: Krebs, M
last_name: Krebs
- first_name: K
full_name: Schumacher, K
last_name: Schumacher
- first_name: MK
full_name: Nowack, MK
last_name: Nowack
- first_name: W
full_name: Moeder, W
last_name: Moeder
- first_name: K
full_name: Yoshioka, K
last_name: Yoshioka
- first_name: D
full_name: Van Damme, D
last_name: Van Damme
- first_name: I
full_name: De Smet, I
last_name: De Smet
- first_name: D
full_name: Geelen, D
last_name: Geelen
- first_name: T
full_name: Beeckman, T
last_name: Beeckman
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: A
full_name: Costa, A
last_name: Costa
- first_name: S
full_name: Vanneste, S
last_name: Vanneste
citation:
ama: Wang R, Himschoot E, Grenzi M, et al. Auxin analog-induced Ca2+ signaling is
independent of inhibition of endosomal aggregation in Arabidopsis roots. Journal
of Experimental Botany. 2022;73(8). doi:10.1093/jxb/erac019
apa: Wang, R., Himschoot, E., Grenzi, M., Chen, J., Safi, A., Krebs, M., … Vanneste,
S. (2022). Auxin analog-induced Ca2+ signaling is independent of inhibition of
endosomal aggregation in Arabidopsis roots. Journal of Experimental Botany.
Oxford Academic. https://doi.org/10.1093/jxb/erac019
chicago: Wang, R, E Himschoot, M Grenzi, J Chen, A Safi, M Krebs, K Schumacher,
et al. “Auxin Analog-Induced Ca2+ Signaling Is Independent of Inhibition of Endosomal
Aggregation in Arabidopsis Roots.” Journal of Experimental Botany. Oxford
Academic, 2022. https://doi.org/10.1093/jxb/erac019.
ieee: R. Wang et al., “Auxin analog-induced Ca2+ signaling is independent
of inhibition of endosomal aggregation in Arabidopsis roots,” Journal of Experimental
Botany, vol. 73, no. 8. Oxford Academic, 2022.
ista: Wang R, Himschoot E, Grenzi M, Chen J, Safi A, Krebs M, Schumacher K, Nowack
M, Moeder W, Yoshioka K, Van Damme D, De Smet I, Geelen D, Beeckman T, Friml J,
Costa A, Vanneste S. 2022. Auxin analog-induced Ca2+ signaling is independent
of inhibition of endosomal aggregation in Arabidopsis roots. Journal of Experimental
Botany. 73(8), erac019.
mla: Wang, R., et al. “Auxin Analog-Induced Ca2+ Signaling Is Independent of Inhibition
of Endosomal Aggregation in Arabidopsis Roots.” Journal of Experimental Botany,
vol. 73, no. 8, erac019, Oxford Academic, 2022, doi:10.1093/jxb/erac019.
short: R. Wang, E. Himschoot, M. Grenzi, J. Chen, A. Safi, M. Krebs, K. Schumacher,
M. Nowack, W. Moeder, K. Yoshioka, D. Van Damme, I. De Smet, D. Geelen, T. Beeckman,
J. Friml, A. Costa, S. Vanneste, Journal of Experimental Botany 73 (2022).
date_created: 2022-02-03T09:19:01Z
date_published: 2022-04-18T00:00:00Z
date_updated: 2023-08-02T14:07:58Z
day: '18'
department:
- _id: JiFr
doi: 10.1093/jxb/erac019
ec_funded: 1
external_id:
isi:
- '000764220900001'
pmid:
- '35085386'
intvolume: ' 73'
isi: 1
issue: '8'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://biblio.ugent.be/publication/8738721
month: '04'
oa: 1
oa_version: Submitted Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Journal of Experimental Botany
publication_identifier:
eissn:
- 1460-2431
issn:
- 0022-0957
publication_status: published
publisher: Oxford Academic
quality_controlled: '1'
scopus_import: '1'
status: public
title: Auxin analog-induced Ca2+ signaling is independent of inhibition of endosomal
aggregation in Arabidopsis roots
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 73
year: '2022'
...
---
_id: '10719'
abstract:
- lang: eng
text: Auxin, one of the first identified and most widely studied phytohormones,
has been and will remain a hot topic in plant biology. After more than a century
of passionate exploration, the mysteries of its synthesis, transport, signaling,
and metabolism have largely been unlocked. Due to the rapid development of new
technologies, new methods, and new genetic materials, the study of auxin has entered
the fast lane over the past 30 years. Here, we highlight advances in understanding
auxin signaling, including auxin perception, rapid auxin responses, TRANSPORT
INHIBITOR RESPONSE 1 and AUXIN SIGNALING F-boxes (TIR1/AFBs)-mediated transcriptional
and non-transcriptional branches, and the epigenetic regulation of auxin signaling.
We also focus on feedback inhibition mechanisms that prevent the over-amplification
of auxin signals. In addition, we cover the TRANSMEMBRANE KINASEs (TMKs)-mediated
non-canonical signaling, which converges with TIR1/AFBs-mediated transcriptional
regulation to coordinate plant growth and development. The identification of additional
auxin signaling components and their regulation will continue to open new avenues
of research in this field, leading to an increasingly deeper, more comprehensive
understanding of how auxin signals are interpreted at the cellular level to regulate
plant growth and development.
acknowledgement: "This research was financially supported by the National Natural
Science Foundation of China and the Israel Science Foundation (NSFC-ISF; 32061143005),
National Natural Science Foundation of China (32000225), Natural Science Foundation
of Shandong Province (ZR2020QC036), and China Postdoctoral Science Foundation (2020M682165).\r\n"
article_processing_charge: No
article_type: review
author:
- first_name: Z
full_name: Yu, Z
last_name: Yu
- first_name: F
full_name: Zhang, F
last_name: Zhang
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Z
full_name: Ding, Z
last_name: Ding
citation:
ama: 'Yu Z, Zhang F, Friml J, Ding Z. Auxin signaling: Research advances over the
past 30 years. Journal of Integrative Plant Biology. 2022;64(2):371-392.
doi:10.1111/jipb.13225'
apa: 'Yu, Z., Zhang, F., Friml, J., & Ding, Z. (2022). Auxin signaling: Research
advances over the past 30 years. Journal of Integrative Plant Biology.
Wiley. https://doi.org/10.1111/jipb.13225'
chicago: 'Yu, Z, F Zhang, Jiří Friml, and Z Ding. “Auxin Signaling: Research Advances
over the Past 30 Years.” Journal of Integrative Plant Biology. Wiley, 2022.
https://doi.org/10.1111/jipb.13225.'
ieee: 'Z. Yu, F. Zhang, J. Friml, and Z. Ding, “Auxin signaling: Research advances
over the past 30 years,” Journal of Integrative Plant Biology, vol. 64,
no. 2. Wiley, pp. 371–392, 2022.'
ista: 'Yu Z, Zhang F, Friml J, Ding Z. 2022. Auxin signaling: Research advances
over the past 30 years. Journal of Integrative Plant Biology. 64(2), 371–392.'
mla: 'Yu, Z., et al. “Auxin Signaling: Research Advances over the Past 30 Years.”
Journal of Integrative Plant Biology, vol. 64, no. 2, Wiley, 2022, pp.
371–92, doi:10.1111/jipb.13225.'
short: Z. Yu, F. Zhang, J. Friml, Z. Ding, Journal of Integrative Plant Biology
64 (2022) 371–392.
date_created: 2022-02-03T09:52:59Z
date_published: 2022-02-01T00:00:00Z
date_updated: 2023-08-02T14:08:30Z
day: '01'
department:
- _id: JiFr
doi: 10.1111/jipb.13225
external_id:
isi:
- '000761281200011'
pmid:
- '35018726'
intvolume: ' 64'
isi: 1
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1111/jipb.13225
month: '02'
oa: 1
oa_version: Published Version
page: 371-392
pmid: 1
publication: Journal of Integrative Plant Biology
publication_identifier:
eissn:
- 1744-7909
issn:
- 1672-9072
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Auxin signaling: Research advances over the past 30 years'
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 64
year: '2022'
...
---
_id: '10768'
abstract:
- lang: eng
text: Among the most fascinated properties of the plant hormone auxin is its ability
to promote formation of its own directional transport routes. These gradually
narrowing auxin channels form from the auxin source toward the sink and involve
coordinated, collective polarization of individual cells. Once established, the
channels provide positional information, along which new vascular strands form,
for example, during organogenesis, regeneration, or leave venation. The main prerequisite
of this still mysterious auxin canalization mechanism is a feedback between auxin
signaling and its directional transport. This is manifested by auxin-induced re-arrangements
of polar, subcellular localization of PIN-FORMED (PIN) auxin exporters. Immanent
open questions relate to how position of auxin source and sink as well as tissue
context are sensed and translated into tissue polarization and how cells communicate
to polarize coordinately. Recently, identification of the first molecular players
opens new avenues into molecular studies of this intriguing example of self-organizing
plant development.
acknowledgement: The authors apologize to those researchers whose work was not cited.
In addition, exciting topics such as PIN polarization in context of phyllotaxis,
shoot branching and termination of gravitropic bending, or role of additional auxin
transporters could not have been included owing to lack of space. This work was
supported by the Czech Science Foundation GAČR (GA18-26981S). The authors also acknowledge
the EMBO for supporting J.H. with a long-term fellowship (ALTF217-2021).
article_number: '102174'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Jakub
full_name: Hajny, Jakub
id: 4800CC20-F248-11E8-B48F-1D18A9856A87
last_name: Hajny
orcid: 0000-0003-2140-7195
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Hajny J, Tan S, Friml J. Auxin canalization: From speculative models toward
molecular players. Current Opinion in Plant Biology. 2022;65(2). doi:10.1016/j.pbi.2022.102174'
apa: 'Hajny, J., Tan, S., & Friml, J. (2022). Auxin canalization: From speculative
models toward molecular players. Current Opinion in Plant Biology. Elsevier.
https://doi.org/10.1016/j.pbi.2022.102174'
chicago: 'Hajny, Jakub, Shutang Tan, and Jiří Friml. “Auxin Canalization: From Speculative
Models toward Molecular Players.” Current Opinion in Plant Biology. Elsevier,
2022. https://doi.org/10.1016/j.pbi.2022.102174.'
ieee: 'J. Hajny, S. Tan, and J. Friml, “Auxin canalization: From speculative models
toward molecular players,” Current Opinion in Plant Biology, vol. 65, no.
2. Elsevier, 2022.'
ista: 'Hajny J, Tan S, Friml J. 2022. Auxin canalization: From speculative models
toward molecular players. Current Opinion in Plant Biology. 65(2), 102174.'
mla: 'Hajny, Jakub, et al. “Auxin Canalization: From Speculative Models toward Molecular
Players.” Current Opinion in Plant Biology, vol. 65, no. 2, 102174, Elsevier,
2022, doi:10.1016/j.pbi.2022.102174.'
short: J. Hajny, S. Tan, J. Friml, Current Opinion in Plant Biology 65 (2022).
date_created: 2022-02-20T23:01:32Z
date_published: 2022-02-01T00:00:00Z
date_updated: 2023-08-02T14:29:12Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.pbi.2022.102174
external_id:
isi:
- '000758724700004'
pmid:
- '35123880'
file:
- access_level: open_access
checksum: f1ee02b6fb4200934eeb31fa69120885
content_type: application/pdf
creator: dernst
date_created: 2022-03-10T13:34:09Z
date_updated: 2022-03-10T13:34:09Z
file_id: '10844'
file_name: 2022_CurrentOpPlantBiology_Hajny.pdf
file_size: 820322
relation: main_file
success: 1
file_date_updated: 2022-03-10T13:34:09Z
has_accepted_license: '1'
intvolume: ' 65'
isi: 1
issue: '2'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
publication: Current Opinion in Plant Biology
publication_identifier:
issn:
- 1369-5266
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Auxin canalization: From speculative models toward molecular players'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 65
year: '2022'
...
---
_id: '10841'
abstract:
- lang: eng
text: In eukaryotes, clathrin-coated vesicles (CCVs) facilitate the internalization
of material from the cell surface as well as the movement of cargo in post-Golgi
trafficking pathways. This diversity of functions is partially provided by multiple
monomeric and multimeric clathrin adaptor complexes that provide compartment and
cargo selectivity. The adaptor-protein assembly polypeptide-1 (AP-1) complex operates
as part of the secretory pathway at the trans-Golgi network (TGN), while the AP-2
complex and the TPLATE complex jointly operate at the plasma membrane to execute
clathrin-mediated endocytosis. Key to our further understanding of clathrin-mediated
trafficking in plants will be the comprehensive identification and characterization
of the network of evolutionarily conserved and plant-specific core and accessory
machinery involved in the formation and targeting of CCVs. To facilitate these
studies, we have analyzed the proteome of enriched TGN/early endosome-derived
and endocytic CCVs isolated from dividing and expanding suspension-cultured Arabidopsis
(Arabidopsis thaliana) cells. Tandem mass spectrometry analysis results were validated
by differential chemical labeling experiments to identify proteins co-enriching
with CCVs. Proteins enriched in CCVs included previously characterized CCV components
and cargos such as the vacuolar sorting receptors in addition to conserved and
plant-specific components whose function in clathrin-mediated trafficking has
not been previously defined. Notably, in addition to AP-1 and AP-2, all subunits
of the AP-4 complex, but not AP-3 or AP-5, were found to be in high abundance
in the CCV proteome. The association of AP-4 with suspension-cultured Arabidopsis
CCVs is further supported via additional biochemical data.
acknowledged_ssus:
- _id: EM-Fac
acknowledgement: 'The authors would like to acknowledge the VIB Proteomics Core Facility
(VIB-UGent Center for Medical Biotechnology in Ghent, Belgium) and the Research
Technology Support Facility Proteomics Core (Michigan State University in East Lansing,
Michigan) for sample analysis, as well as the University of Wisconsin Biotechnology
Center Mass Spectrometry Core Facility (Madison, WI) for help with data processing.
Additionally, we are grateful to Sue Weintraub (UT Health San Antonio) and Sydney
Thomas (UW- Madison) for assistance with data analysis. This research was supported
by grants to S.Y.B. from the National Science Foundation (Nos. 1121998 and 1614915)
and a Vilas Associate Award (University of Wisconsin, Madison, Graduate School);
to J.P. from the National Natural Science Foundation of China (Nos. 91754104, 31820103008,
and 31670283); to I.H. from the National Research Foundation of Korea (No. 2019R1A2B5B03099982).
This research was also supported by the Scientific Service Units (SSU) of IST Austria
through resources provided by the Electron microscopy Facility (EMF). A.J. is supported
by funding from the Austrian Science Fund (FWF): I3630B25 to J.F. A.H. is supported
by funding from the National Science Foundation (NSF IOS Nos. 1025837 and 1147032).'
article_processing_charge: No
article_type: original
author:
- first_name: DA
full_name: Dahhan, DA
last_name: Dahhan
- first_name: GD
full_name: Reynolds, GD
last_name: Reynolds
- first_name: JJ
full_name: Cárdenas, JJ
last_name: Cárdenas
- first_name: D
full_name: Eeckhout, D
last_name: Eeckhout
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: K
full_name: Yperman, K
last_name: Yperman
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: N
full_name: Vang, N
last_name: Vang
- first_name: X
full_name: Yan, X
last_name: Yan
- first_name: I
full_name: Hwang, I
last_name: Hwang
- first_name: A
full_name: Heese, A
last_name: Heese
- first_name: G
full_name: De Jaeger, G
last_name: De Jaeger
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: D
full_name: Van Damme, D
last_name: Van Damme
- first_name: J
full_name: Pan, J
last_name: Pan
- first_name: SY
full_name: Bednarek, SY
last_name: Bednarek
citation:
ama: Dahhan D, Reynolds G, Cárdenas J, et al. Proteomic characterization of isolated
Arabidopsis clathrin-coated vesicles reveals evolutionarily conserved and plant-specific
components. Plant Cell. 2022;34(6):2150-2173. doi:10.1093/plcell/koac071
apa: Dahhan, D., Reynolds, G., Cárdenas, J., Eeckhout, D., Johnson, A. J., Yperman,
K., … Bednarek, S. (2022). Proteomic characterization of isolated Arabidopsis
clathrin-coated vesicles reveals evolutionarily conserved and plant-specific components.
Plant Cell. Oxford Academic. https://doi.org/10.1093/plcell/koac071
chicago: Dahhan, DA, GD Reynolds, JJ Cárdenas, D Eeckhout, Alexander J Johnson,
K Yperman, Walter Kaufmann, et al. “Proteomic Characterization of Isolated Arabidopsis
Clathrin-Coated Vesicles Reveals Evolutionarily Conserved and Plant-Specific Components.”
Plant Cell. Oxford Academic, 2022. https://doi.org/10.1093/plcell/koac071.
ieee: D. Dahhan et al., “Proteomic characterization of isolated Arabidopsis
clathrin-coated vesicles reveals evolutionarily conserved and plant-specific components,”
Plant Cell, vol. 34, no. 6. Oxford Academic, pp. 2150–2173, 2022.
ista: Dahhan D, Reynolds G, Cárdenas J, Eeckhout D, Johnson AJ, Yperman K, Kaufmann
W, Vang N, Yan X, Hwang I, Heese A, De Jaeger G, Friml J, Van Damme D, Pan J,
Bednarek S. 2022. Proteomic characterization of isolated Arabidopsis clathrin-coated
vesicles reveals evolutionarily conserved and plant-specific components. Plant
Cell. 34(6), 2150–2173.
mla: Dahhan, DA, et al. “Proteomic Characterization of Isolated Arabidopsis Clathrin-Coated
Vesicles Reveals Evolutionarily Conserved and Plant-Specific Components.” Plant
Cell, vol. 34, no. 6, Oxford Academic, 2022, pp. 2150–73, doi:10.1093/plcell/koac071.
short: D. Dahhan, G. Reynolds, J. Cárdenas, D. Eeckhout, A.J. Johnson, K. Yperman,
W. Kaufmann, N. Vang, X. Yan, I. Hwang, A. Heese, G. De Jaeger, J. Friml, D. Van
Damme, J. Pan, S. Bednarek, Plant Cell 34 (2022) 2150–2173.
date_created: 2022-03-08T13:47:51Z
date_published: 2022-06-01T00:00:00Z
date_updated: 2023-08-02T14:46:48Z
day: '01'
department:
- _id: JiFr
- _id: EM-Fac
doi: 10.1093/plcell/koac071
external_id:
isi:
- '000767438800001'
pmid:
- '35218346'
intvolume: ' 34'
isi: 1
issue: '6'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2021.09.16.460678
month: '06'
oa: 1
oa_version: Preprint
page: 2150-2173
pmid: 1
project:
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: Plant Cell
publication_identifier:
eissn:
- 1532-298x
issn:
- 1040-4651
publication_status: published
publisher: Oxford Academic
quality_controlled: '1'
scopus_import: '1'
status: public
title: Proteomic characterization of isolated Arabidopsis clathrin-coated vesicles
reveals evolutionarily conserved and plant-specific components
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 34
year: '2022'
...
---
_id: '10888'
abstract:
- lang: eng
text: Despite the growing interest in using chemical genetics in plant research,
small molecule target identification remains a major challenge. The cellular thermal
shift assay coupled with high-resolution mass spectrometry (CETSA MS) that monitors
changes in the thermal stability of proteins caused by their interactions with
small molecules, other proteins, or posttranslational modifications, allows the
discovery of drug targets or the study of protein–metabolite and protein–protein
interactions mainly in mammalian cells. To showcase the applicability of this
method in plants, we applied CETSA MS to intact Arabidopsis thaliana cells and
identified the thermal proteome of the plant-specific glycogen synthase kinase
3 (GSK3) inhibitor, bikinin. A comparison between the thermal and the phosphoproteomes
of bikinin revealed the auxin efflux carrier PIN-FORMED1 (PIN1) as a substrate
of the Arabidopsis GSK3s that negatively regulate the brassinosteroid signaling.
We established that PIN1 phosphorylation by the GSK3s is essential for maintaining
its intracellular polarity that is required for auxin-mediated regulation of vascular
patterning in the leaf, thus revealing cross-talk between brassinosteroid and
auxin signaling.
acknowledgement: "We thank Yanhai Yin for providing the anti-BES1 antibody, Johan
Winne and Brenda Callebaut for synthesizing bikinin, Yuki Kondo and Hiroo Fukuda
for published materials, Tomasz Nodzy\x03nski for useful advice, and Martine De
Cock for help in preparing the manuscript. This\r\nwork was supported by the China
Scholarship Council for predoctoral (Q.L. and X.X.) and postdoctoral (Y.Z.) fellowships;
the Agency for Innovation by Science and Technology for a predoctoral fellowship
(W.D.); the Research Foundation-Flanders, Projects G009018N and G002121N (E.R.);
and the VIB TechWatch Fund (E.R.)."
article_number: e2118220119
article_processing_charge: No
article_type: original
author:
- first_name: Qing
full_name: Lu, Qing
last_name: Lu
- first_name: Yonghong
full_name: Zhang, Yonghong
last_name: Zhang
- first_name: Joakim
full_name: Hellner, Joakim
last_name: Hellner
- first_name: Caterina
full_name: Giannini, Caterina
id: e3fdddd5-f6e0-11ea-865d-ca99ee6367f4
last_name: Giannini
- first_name: Xiangyu
full_name: Xu, Xiangyu
last_name: Xu
- first_name: Jarne
full_name: Pauwels, Jarne
last_name: Pauwels
- first_name: Qian
full_name: Ma, Qian
last_name: Ma
- first_name: Wim
full_name: Dejonghe, Wim
last_name: Dejonghe
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
- first_name: Brigitte
full_name: Van De Cotte, Brigitte
last_name: Van De Cotte
- first_name: Francis
full_name: Impens, Francis
last_name: Impens
- first_name: Kris
full_name: Gevaert, Kris
last_name: Gevaert
- first_name: Ive
full_name: De Smet, Ive
last_name: De Smet
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Daniel Martinez
full_name: Molina, Daniel Martinez
last_name: Molina
- first_name: Eugenia
full_name: Russinova, Eugenia
last_name: Russinova
citation:
ama: Lu Q, Zhang Y, Hellner J, et al. Proteome-wide cellular thermal shift assay
reveals unexpected cross-talk between brassinosteroid and auxin signaling. Proceedings
of the National Academy of Sciences of the United States of America. 2022;119(11).
doi:10.1073/pnas.2118220119
apa: Lu, Q., Zhang, Y., Hellner, J., Giannini, C., Xu, X., Pauwels, J., … Russinova,
E. (2022). Proteome-wide cellular thermal shift assay reveals unexpected cross-talk
between brassinosteroid and auxin signaling. Proceedings of the National Academy
of Sciences of the United States of America. Proceedings of the National Academy
of Sciences. https://doi.org/10.1073/pnas.2118220119
chicago: Lu, Qing, Yonghong Zhang, Joakim Hellner, Caterina Giannini, Xiangyu Xu,
Jarne Pauwels, Qian Ma, et al. “Proteome-Wide Cellular Thermal Shift Assay Reveals Unexpected
Cross-Talk between Brassinosteroid and Auxin Signaling.” Proceedings of the
National Academy of Sciences of the United States of America. Proceedings
of the National Academy of Sciences, 2022. https://doi.org/10.1073/pnas.2118220119.
ieee: Q. Lu et al., “Proteome-wide cellular thermal shift assay reveals unexpected
cross-talk between brassinosteroid and auxin signaling,” Proceedings of the
National Academy of Sciences of the United States of America, vol. 119, no.
11. Proceedings of the National Academy of Sciences, 2022.
ista: Lu Q, Zhang Y, Hellner J, Giannini C, Xu X, Pauwels J, Ma Q, Dejonghe W, Han
H, Van De Cotte B, Impens F, Gevaert K, De Smet I, Friml J, Molina DM, Russinova
E. 2022. Proteome-wide cellular thermal shift assay reveals unexpected cross-talk
between brassinosteroid and auxin signaling. Proceedings of the National Academy
of Sciences of the United States of America. 119(11), e2118220119.
mla: Lu, Qing, et al. “Proteome-Wide Cellular Thermal Shift Assay Reveals Unexpected
Cross-Talk between Brassinosteroid and Auxin Signaling.” Proceedings of the
National Academy of Sciences of the United States of America, vol. 119, no.
11, e2118220119, Proceedings of the National Academy of Sciences, 2022, doi:10.1073/pnas.2118220119.
short: Q. Lu, Y. Zhang, J. Hellner, C. Giannini, X. Xu, J. Pauwels, Q. Ma, W. Dejonghe,
H. Han, B. Van De Cotte, F. Impens, K. Gevaert, I. De Smet, J. Friml, D.M. Molina,
E. Russinova, Proceedings of the National Academy of Sciences of the United States
of America 119 (2022).
date_created: 2022-03-20T23:01:39Z
date_published: 2022-03-07T00:00:00Z
date_updated: 2023-08-03T06:06:27Z
day: '07'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1073/pnas.2118220119
external_id:
isi:
- '000771756300008'
pmid:
- '35254915'
file:
- access_level: open_access
checksum: 83e0fea7919570d0b519b41193342571
content_type: application/pdf
creator: dernst
date_created: 2022-03-21T09:19:47Z
date_updated: 2022-03-21T09:19:47Z
file_id: '10910'
file_name: 2022_PNAS_Lu.pdf
file_size: 2169534
relation: main_file
success: 1
file_date_updated: 2022-03-21T09:19:47Z
has_accepted_license: '1'
intvolume: ' 119'
isi: 1
issue: '11'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
publication: Proceedings of the National Academy of Sciences of the United States
of America
publication_identifier:
eissn:
- 1091-6490
publication_status: published
publisher: Proceedings of the National Academy of Sciences
quality_controlled: '1'
scopus_import: '1'
status: public
title: Proteome-wide cellular thermal shift assay reveals unexpected cross-talk between
brassinosteroid and auxin signaling
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 119
year: '2022'
...
---
_id: '11589'
abstract:
- lang: eng
text: Calcium-dependent protein kinases (CPK) are key components of a wide array
of signaling pathways, translating stress and nutrient signaling into the modulation
of cellular processes such as ion transport and transcription. However, not much
is known about CPKs in endomembrane trafficking. Here, we screened for CPKs that
impact on root growth and gravitropism, by overexpressing constitutively active
forms of CPKs under the control of an inducible promoter in Arabidopsis thaliana.
We found that inducible overexpression of an constitutive active CPK30 (CA-CPK30)
resulted in a loss of root gravitropism and ectopic auxin accumulation in the
root tip. Immunolocalization revealed that CA-CPK30 roots have reduced PIN protein
levels, PIN1 polarity defects and impaired Brefeldin A (BFA)-sensitive trafficking.
Moreover, FM4-64 uptake was reduced, indicative of a defect in endocytosis. The
effects on BFA-sensitive trafficking were not specific to PINs, as BFA could not
induce aggregation of ARF1- and CHC-labeled endosomes in CA-CPK30. Interestingly,
the interference with BFA-body formation, could be reverted by increasing the
extracellular pH, indicating a pH-dependence of this CA-CPK30 effect. Altogether,
our data reveal an important role for CPK30 in root growth regulation and endomembrane
trafficking in Arabidopsis thaliana.
acknowledgement: "RW and JC predoctoral fellows that were supported by the Chinese
Science Counsil. The IPS2 benefits from the support of the LabEx Saclay Plant Sciences-SPS
(ANR-10-LABX-0040-SPS).\r\nWe thank Jen Sheen for establishing and generously sharing
the CKP family clone sets, and for providing useful feedback on the manuscript."
article_number: '862398'
article_processing_charge: No
article_type: original
author:
- first_name: Ren
full_name: Wang, Ren
last_name: Wang
- first_name: Ellie
full_name: Himschoot, Ellie
last_name: Himschoot
- first_name: Jian
full_name: Chen, Jian
last_name: Chen
- first_name: Marie
full_name: Boudsocq, Marie
last_name: Boudsocq
- first_name: Danny
full_name: Geelen, Danny
last_name: Geelen
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Tom
full_name: Beeckman, Tom
last_name: Beeckman
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
citation:
ama: Wang R, Himschoot E, Chen J, et al. Constitutive active CPK30 interferes with
root growth and endomembrane trafficking in Arabidopsis thaliana. Frontiers
in Plant Science. 2022;13. doi:10.3389/fpls.2022.862398
apa: Wang, R., Himschoot, E., Chen, J., Boudsocq, M., Geelen, D., Friml, J., … Vanneste,
S. (2022). Constitutive active CPK30 interferes with root growth and endomembrane
trafficking in Arabidopsis thaliana. Frontiers in Plant Science. Frontiers.
https://doi.org/10.3389/fpls.2022.862398
chicago: Wang, Ren, Ellie Himschoot, Jian Chen, Marie Boudsocq, Danny Geelen, Jiří
Friml, Tom Beeckman, and Steffen Vanneste. “Constitutive Active CPK30 Interferes
with Root Growth and Endomembrane Trafficking in Arabidopsis Thaliana.” Frontiers
in Plant Science. Frontiers, 2022. https://doi.org/10.3389/fpls.2022.862398.
ieee: R. Wang et al., “Constitutive active CPK30 interferes with root growth
and endomembrane trafficking in Arabidopsis thaliana,” Frontiers in Plant Science,
vol. 13. Frontiers, 2022.
ista: Wang R, Himschoot E, Chen J, Boudsocq M, Geelen D, Friml J, Beeckman T, Vanneste
S. 2022. Constitutive active CPK30 interferes with root growth and endomembrane
trafficking in Arabidopsis thaliana. Frontiers in Plant Science. 13, 862398.
mla: Wang, Ren, et al. “Constitutive Active CPK30 Interferes with Root Growth and
Endomembrane Trafficking in Arabidopsis Thaliana.” Frontiers in Plant Science,
vol. 13, 862398, Frontiers, 2022, doi:10.3389/fpls.2022.862398.
short: R. Wang, E. Himschoot, J. Chen, M. Boudsocq, D. Geelen, J. Friml, T. Beeckman,
S. Vanneste, Frontiers in Plant Science 13 (2022).
date_created: 2022-07-17T22:01:54Z
date_published: 2022-06-16T00:00:00Z
date_updated: 2023-08-03T12:01:47Z
day: '16'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.3389/fpls.2022.862398
external_id:
isi:
- '000819250500001'
pmid:
- '35783951'
file:
- access_level: open_access
checksum: 95313515637c0f84de591d204375d764
content_type: application/pdf
creator: dernst
date_created: 2022-07-18T08:05:15Z
date_updated: 2022-07-18T08:05:15Z
file_id: '11596'
file_name: 2022_FrontiersPlantScience_Wang.pdf
file_size: 5040638
relation: main_file
success: 1
file_date_updated: 2022-07-18T08:05:15Z
has_accepted_license: '1'
intvolume: ' 13'
isi: 1
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
pmid: 1
publication: Frontiers in Plant Science
publication_identifier:
eissn:
- 1664-462X
publication_status: published
publisher: Frontiers
quality_controlled: '1'
related_material:
link:
- relation: erratum
url: https://doi.org/10.3389/fpls.2022.1100792
scopus_import: '1'
status: public
title: Constitutive active CPK30 interferes with root growth and endomembrane trafficking
in Arabidopsis thaliana
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 13
year: '2022'
...
---
_id: '11723'
abstract:
- lang: eng
text: Plant cell growth responds rapidly to various stimuli, adapting architecture
to environmental changes. Two major endogenous signals regulating growth are the
phytohormone auxin and the secreted peptides rapid alkalinization factors (RALFs).
Both trigger very rapid cellular responses and also exert long-term effects [Du
et al., Annu. Rev. Plant Biol. 71, 379–402 (2020); Blackburn et al., Plant Physiol.
182, 1657–1666 (2020)]. However, the way, in which these distinct signaling pathways
converge to regulate growth, remains unknown. Here, using vertical confocal microscopy
combined with a microfluidic chip, we addressed the mechanism of RALF action on
growth. We observed correlation between RALF1-induced rapid Arabidopsis thaliana
root growth inhibition and apoplast alkalinization during the initial phase of
the response, and revealed that RALF1 reversibly inhibits primary root growth
through apoplast alkalinization faster than within 1 min. This rapid apoplast
alkalinization was the result of RALF1-induced net H+ influx and was mediated
by the receptor FERONIA (FER). Furthermore, we investigated the cross-talk between
RALF1 and the auxin signaling pathways during root growth regulation. The results
showed that RALF-FER signaling triggered auxin signaling with a delay of approximately
1 h by up-regulating auxin biosynthesis, thus contributing to sustained RALF1-induced
growth inhibition. This biphasic RALF1 action on growth allows plants to respond
rapidly to environmental stimuli and also reprogram growth and development in
the long term.
acknowledgement: We thank Sarah M. Assmann, Kris Vissenberg, and Nadine Paris for
kindly sharing seeds; Matyáš Fendrych for initiating this project and providing
constant support; Lukas Fiedler for revising the manuscript; and Huibin Han and
Arseny Savin for contributing to genotyping. This work was supported by the Austrian
Science Fund (FWF) I 3630-B25 (to J.F.) and the Doctoral Fellowship Progrmme of
the Austrian Academy of Sciences (to L.L.) We also acknowledge Taif University Researchers
Supporting Project TURSP-HC2021/02 and funding “Plants as a tool for sustainable
global development (no. CZ.02.1.01/0.0/0.0/16_019/0000827).”
article_number: e2121058119
article_processing_charge: No
article_type: original
author:
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Huihuang
full_name: Chen, Huihuang
id: 83c96512-15b2-11ec-abd3-b7eede36184f
last_name: Chen
- first_name: Saqer S.
full_name: Alotaibi, Saqer S.
last_name: Alotaibi
- first_name: Aleš
full_name: Pěnčík, Aleš
last_name: Pěnčík
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Ondřej
full_name: Novák, Ondřej
last_name: Novák
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Li L, Chen H, Alotaibi SS, et al. RALF1 peptide triggers biphasic root growth
inhibition upstream of auxin biosynthesis. Proceedings of the National Academy
of Sciences. 2022;119(31). doi:10.1073/pnas.2121058119
apa: Li, L., Chen, H., Alotaibi, S. S., Pěnčík, A., Adamowski, M., Novák, O., &
Friml, J. (2022). RALF1 peptide triggers biphasic root growth inhibition upstream
of auxin biosynthesis. Proceedings of the National Academy of Sciences.
Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.2121058119
chicago: Li, Lanxin, Huihuang Chen, Saqer S. Alotaibi, Aleš Pěnčík, Maciek Adamowski,
Ondřej Novák, and Jiří Friml. “RALF1 Peptide Triggers Biphasic Root Growth Inhibition
Upstream of Auxin Biosynthesis.” Proceedings of the National Academy of Sciences.
Proceedings of the National Academy of Sciences, 2022. https://doi.org/10.1073/pnas.2121058119.
ieee: L. Li et al., “RALF1 peptide triggers biphasic root growth inhibition
upstream of auxin biosynthesis,” Proceedings of the National Academy of Sciences,
vol. 119, no. 31. Proceedings of the National Academy of Sciences, 2022.
ista: Li L, Chen H, Alotaibi SS, Pěnčík A, Adamowski M, Novák O, Friml J. 2022.
RALF1 peptide triggers biphasic root growth inhibition upstream of auxin biosynthesis.
Proceedings of the National Academy of Sciences. 119(31), e2121058119.
mla: Li, Lanxin, et al. “RALF1 Peptide Triggers Biphasic Root Growth Inhibition
Upstream of Auxin Biosynthesis.” Proceedings of the National Academy of Sciences,
vol. 119, no. 31, e2121058119, Proceedings of the National Academy of Sciences,
2022, doi:10.1073/pnas.2121058119.
short: L. Li, H. Chen, S.S. Alotaibi, A. Pěnčík, M. Adamowski, O. Novák, J. Friml,
Proceedings of the National Academy of Sciences 119 (2022).
date_created: 2022-08-04T20:06:49Z
date_published: 2022-07-25T00:00:00Z
date_updated: 2023-08-03T12:43:53Z
day: '25'
ddc:
- '580'
department:
- _id: GradSch
- _id: JiFr
doi: 10.1073/pnas.2121058119
external_id:
isi:
- '000881496900002'
pmid:
- '35878023'
file:
- access_level: open_access
checksum: ae6f19b0d9efba6687f9e4dc1bab1d6e
content_type: application/pdf
creator: dernst
date_created: 2022-08-08T07:42:09Z
date_updated: 2022-08-08T07:42:09Z
file_id: '11747'
file_name: 2022_PNAS_Li.pdf
file_size: 2506262
relation: main_file
success: 1
file_date_updated: 2022-08-08T07:42:09Z
has_accepted_license: '1'
intvolume: ' 119'
isi: 1
issue: '31'
keyword:
- Multidisciplinary
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 26B4D67E-B435-11E9-9278-68D0E5697425
grant_number: '25351'
name: 'A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated
Rapid Growth Inhibition in Arabidopsis Root'
publication: Proceedings of the National Academy of Sciences
publication_identifier:
eissn:
- 1091-6490
issn:
- 0027-8424
publication_status: published
publisher: Proceedings of the National Academy of Sciences
quality_controlled: '1'
scopus_import: '1'
status: public
title: RALF1 peptide triggers biphasic root growth inhibition upstream of auxin biosynthesis
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 119
year: '2022'
...
---
_id: '12053'
abstract:
- lang: eng
text: Strigolactones (SLs) are a class of phytohormones that regulate plant shoot
branching and adventitious root development. However, little is known regarding
the role of SLs in controlling the behavior of the smallest unit of the organism,
the single cell. Here, taking advantage of a classic single-cell model offered
by the cotton (Gossypium hirsutum) fiber cell, we show that SLs, whose biosynthesis
is fine-tuned by gibberellins (GAs), positively regulate cell elongation and cell
wall thickness by promoting the biosynthesis of very-long-chain fatty acids (VLCFAs)
and cellulose, respectively. Furthermore, we identified two layers of transcription
factors (TFs) involved in the hierarchical regulation of this GA-SL crosstalk.
The top-layer TF GROWTH-REGULATING FACTOR 4 (GhGRF4) directly activates expression
of the SL biosynthetic gene DWARF27 (D27) to increase SL accumulation in fiber
cells and GAs induce GhGRF4 expression. SLs induce the expression of four second-layer
TF genes (GhNAC100-2, GhBLH51, GhGT2, and GhB9SHZ1), which transmit SL signals
downstream to two ketoacyl-CoA synthase genes (KCS) and three cellulose synthase
(CesA) genes by directly activating their transcription. Finally, the KCS and
CesA enzymes catalyze the biosynthesis of very long chain fatty acids and cellulose,
respectively, to regulate development of high-grade cotton fibers. In addition
to providing a theoretical basis for cotton fiber improvement, our results shed
light on SL signaling in plant development at the single-cell level.
acknowledgement: This work was supported by the National Natural Science Foundation
of China (32070549), Shaanxi Youth Entrusted Talent Program (20190205), Fundamental
Research Funds for the Central Universities (GK202002005 and GK202201017), Young
Elite Scientists Sponsorship Program by China Association for Science and Technology
(CAST) (2019-2021QNRC001), State Key Laboratory of Cotton Biology Open Fund (CB2020A12
and CB2021A21) and FWF Stand-alone Project (P29988).
article_processing_charge: No
article_type: original
author:
- first_name: Z
full_name: Tian, Z
last_name: Tian
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: L
full_name: Zhu, L
last_name: Zhu
- first_name: B
full_name: Jiang, B
last_name: Jiang
- first_name: H
full_name: Wang, H
last_name: Wang
- first_name: R
full_name: Gao, R
last_name: Gao
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: G
full_name: Xiao, G
last_name: Xiao
citation:
ama: Tian Z, Zhang Y, Zhu L, et al. Strigolactones act downstream of gibberellins
to regulate fiber cell elongation and cell wall thickness in cotton (Gossypium
hirsutum). The Plant Cell. 2022;34(12):4816-4839. doi:10.1093/plcell/koac270
apa: Tian, Z., Zhang, Y., Zhu, L., Jiang, B., Wang, H., Gao, R., … Xiao, G. (2022).
Strigolactones act downstream of gibberellins to regulate fiber cell elongation
and cell wall thickness in cotton (Gossypium hirsutum). The Plant Cell.
Oxford University Press. https://doi.org/10.1093/plcell/koac270
chicago: Tian, Z, Yuzhou Zhang, L Zhu, B Jiang, H Wang, R Gao, Jiří Friml, and G
Xiao. “Strigolactones Act Downstream of Gibberellins to Regulate Fiber Cell Elongation
and Cell Wall Thickness in Cotton (Gossypium Hirsutum).” The Plant Cell.
Oxford University Press, 2022. https://doi.org/10.1093/plcell/koac270.
ieee: Z. Tian et al., “Strigolactones act downstream of gibberellins to regulate
fiber cell elongation and cell wall thickness in cotton (Gossypium hirsutum),”
The Plant Cell, vol. 34, no. 12. Oxford University Press, pp. 4816–4839,
2022.
ista: Tian Z, Zhang Y, Zhu L, Jiang B, Wang H, Gao R, Friml J, Xiao G. 2022. Strigolactones
act downstream of gibberellins to regulate fiber cell elongation and cell wall
thickness in cotton (Gossypium hirsutum). The Plant Cell. 34(12), 4816–4839.
mla: Tian, Z., et al. “Strigolactones Act Downstream of Gibberellins to Regulate
Fiber Cell Elongation and Cell Wall Thickness in Cotton (Gossypium Hirsutum).”
The Plant Cell, vol. 34, no. 12, Oxford University Press, 2022, pp. 4816–39,
doi:10.1093/plcell/koac270.
short: Z. Tian, Y. Zhang, L. Zhu, B. Jiang, H. Wang, R. Gao, J. Friml, G. Xiao,
The Plant Cell 34 (2022) 4816–4839.
date_created: 2022-09-07T14:19:39Z
date_published: 2022-12-01T00:00:00Z
date_updated: 2023-08-03T13:41:06Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1093/plcell/koac270
external_id:
isi:
- '000852753000001'
pmid:
- '36040191'
file:
- access_level: open_access
checksum: 1c606d9545f29dfca15235f69ad27b58
content_type: application/pdf
creator: dernst
date_created: 2023-01-20T08:29:12Z
date_updated: 2023-01-20T08:29:12Z
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file_date_updated: 2023-01-20T08:29:12Z
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intvolume: ' 34'
isi: 1
issue: '12'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
page: 4816-4839
pmid: 1
project:
- _id: 262EF96E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29988
name: RNA-directed DNA methylation in plant development
publication: The Plant Cell
publication_identifier:
eissn:
- 1532-298X
issn:
- 1040-4651
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
related_material:
link:
- relation: erratum
url: https://doi.org/10.1093/plcell/koac342
scopus_import: '1'
status: public
title: Strigolactones act downstream of gibberellins to regulate fiber cell elongation
and cell wall thickness in cotton (Gossypium hirsutum)
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 34
year: '2022'
...
---
_id: '12052'
abstract:
- lang: eng
text: Directionality in the intercellular transport of the plant hormone auxin is
determined by polar plasma membrane localization of PIN-FORMED (PIN) auxin transport
proteins. However, apart from PIN phosphorylation at conserved motifs, no further
determinants explicitly controlling polar PIN sorting decisions have been identified.
Here we present Arabidopsis WAVY GROWTH 3 (WAV3) and closely related RING-finger
E3 ubiquitin ligases, whose loss-of-function mutants show a striking apical-to-basal
polarity switch in PIN2 localization in root meristem cells. WAV3 E3 ligases function
as essential determinants for PIN polarity, acting independently from PINOID/WAG-dependent
PIN phosphorylation. They antagonize ectopic deposition of de novo synthesized
PIN proteins already immediately following completion of cell division, presumably
via preventing PIN sorting into basal, ARF GEF-mediated trafficking. Our findings
reveal an involvement of E3 ligases in the selective targeting of apically localized
PINs in higher plants.
acknowledgement: We would like to thank Tatsuo Sakai, Marcus Heisler, Toru Fujiwara,
Lucia Strader, Christian Hardtke, Malcolm Bennett, Claus Schwechheimer, Gerd Jürgens
and Remko Offringa for sharing published materials and Alba Grau Gimeno for support.
We are greatly indebted to Bert de Rybel for supporting N.K. and M.G. to work on
the final stages of manuscript preparation as postdocs in his laboratory. A full-length
SOR1 cDNA clone (J090099M14) was obtained from the National Agriculture and Food
Research Organization (NARO, Japan). Support by the Multiscale Imaging Core Facility
at the BOKU is greatly acknowledged. This work has been supported by grants from
the Austrian Science Fund (FWF P25931-B16; P31493-B25 to Christian Luschnig; I3630-B25
to Jiří Friml; P30850-B32 to Barbara Korbei) and from the Swiss National Funds (31003A-165877/1
to Markus Geisler) and the European Union’s Horizon 2020 research and innovation
program (Marie Skłodowska-Curie grant agreement No 885979 to Matouš Glanc).
article_number: '5147'
article_processing_charge: No
article_type: original
author:
- first_name: N
full_name: Konstantinova, N
last_name: Konstantinova
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: R
full_name: Keshkeih, R
last_name: Keshkeih
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: M
full_name: Di Donato, M
last_name: Di Donato
- first_name: K
full_name: Retzer, K
last_name: Retzer
- first_name: J
full_name: Moulinier-Anzola, J
last_name: Moulinier-Anzola
- first_name: M
full_name: Schwihla, M
last_name: Schwihla
- first_name: B
full_name: Korbei, B
last_name: Korbei
- first_name: M
full_name: Geisler, M
last_name: Geisler
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: C
full_name: Luschnig, C
last_name: Luschnig
citation:
ama: Konstantinova N, Hörmayer L, Glanc M, et al. WAVY GROWTH Arabidopsis E3 ubiquitin
ligases affect apical PIN sorting decisions. Nature Communications. 2022;13.
doi:10.1038/s41467-022-32888-8
apa: Konstantinova, N., Hörmayer, L., Glanc, M., Keshkeih, R., Tan, S., Di Donato,
M., … Luschnig, C. (2022). WAVY GROWTH Arabidopsis E3 ubiquitin ligases affect
apical PIN sorting decisions. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-022-32888-8
chicago: Konstantinova, N, Lukas Hörmayer, Matous Glanc, R Keshkeih, Shutang Tan,
M Di Donato, K Retzer, et al. “WAVY GROWTH Arabidopsis E3 Ubiquitin Ligases Affect
Apical PIN Sorting Decisions.” Nature Communications. Springer Nature,
2022. https://doi.org/10.1038/s41467-022-32888-8.
ieee: N. Konstantinova et al., “WAVY GROWTH Arabidopsis E3 ubiquitin ligases
affect apical PIN sorting decisions,” Nature Communications, vol. 13. Springer
Nature, 2022.
ista: Konstantinova N, Hörmayer L, Glanc M, Keshkeih R, Tan S, Di Donato M, Retzer
K, Moulinier-Anzola J, Schwihla M, Korbei B, Geisler M, Friml J, Luschnig C. 2022.
WAVY GROWTH Arabidopsis E3 ubiquitin ligases affect apical PIN sorting decisions.
Nature Communications. 13, 5147.
mla: Konstantinova, N., et al. “WAVY GROWTH Arabidopsis E3 Ubiquitin Ligases Affect
Apical PIN Sorting Decisions.” Nature Communications, vol. 13, 5147, Springer
Nature, 2022, doi:10.1038/s41467-022-32888-8.
short: N. Konstantinova, L. Hörmayer, M. Glanc, R. Keshkeih, S. Tan, M. Di Donato,
K. Retzer, J. Moulinier-Anzola, M. Schwihla, B. Korbei, M. Geisler, J. Friml,
C. Luschnig, Nature Communications 13 (2022).
date_created: 2022-09-07T14:19:26Z
date_published: 2022-09-01T00:00:00Z
date_updated: 2023-08-03T13:40:32Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41467-022-32888-8
external_id:
isi:
- '000848744900004'
pmid:
- '36050482'
file:
- access_level: open_access
checksum: 43336758c89cd6c045839089af070afe
content_type: application/pdf
creator: dernst
date_created: 2022-09-08T07:46:16Z
date_updated: 2022-09-08T07:46:16Z
file_id: '12063'
file_name: 2022_NatureCommunications_Konstantinova.pdf
file_size: 6678579
relation: main_file
success: 1
file_date_updated: 2022-09-08T07:46:16Z
has_accepted_license: '1'
intvolume: ' 13'
isi: 1
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: Nature Communications
publication_identifier:
issn:
- 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- relation: erratum
url: https://doi.org/10.1038/s41467-022-33198-9
status: public
title: WAVY GROWTH Arabidopsis E3 ubiquitin ligases affect apical PIN sorting decisions
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 13
year: '2022'
...
---
_id: '12054'
abstract:
- lang: eng
text: 'Polar auxin transport is unique to plants and coordinates their growth and
development1,2. The PIN-FORMED (PIN) auxin transporters exhibit highly asymmetrical
localizations at the plasma membrane and drive polar auxin transport3,4; however,
their structures and transport mechanisms remain largely unknown. Here, we report
three inward-facing conformation structures of Arabidopsis thaliana PIN1: the
apo state, bound to the natural auxin indole-3-acetic acid (IAA), and in complex
with the polar auxin transport inhibitor N-1-naphthylphthalamic acid (NPA). The
transmembrane domain of PIN1 shares a conserved NhaA fold5. In the substrate-bound
structure, IAA is coordinated by both hydrophobic stacking and hydrogen bonding.
NPA competes with IAA for the same site at the intracellular pocket, but with
a much higher affinity. These findings inform our understanding of the substrate
recognition and transport mechanisms of PINs and set up a framework for future
research on directional auxin transport, one of the most crucial processes underlying
plant development.'
acknowledgement: We thank the Cryo-EM Center of the University of Science and Technology
of China (USTC) and the Center for Biological Imaging (CBI), Institute of Biophysics,
Chinese Academy of Science, for the EM facility support; we thank B. Zhu, X. Huang
and all the other staff members for their technical support on cryo-EM data collection.
We thank J. Ren for his technical support with the transport assays and M. Seeger
for providing the sybody libraries. This work was supported by the Strategic Priority
Research Program of Chinese Academy of Sciences (XDB 37020204 to D.L. and XDB37020103
to Linfeng Sun), National Natural Science Foundation of China (82151215 and 31870726
to D.L., 31900885 to X.L., and 31870732 to Linfeng Sun), Natural Science Foundation
of Anhui Province (2008085MC90 to X.L. and 2008085J15 to Linfeng Sun), the Fundamental
Research Funds for the Central Universities (WK9100000031 to Linfeng Sun), and the
USTC Research Funds of the Double First-Class Initiative (YD9100002004 to Linfeng
Sun). Linfeng Sun is supported by an Outstanding Young Scholar Award from the Qiu
Shi Science and Technologies Foundation, and a Young Scholar Award from the Cyrus
Tang Foundation.
article_processing_charge: No
article_type: original
author:
- first_name: Z
full_name: Yang, Z
last_name: Yang
- first_name: J
full_name: Xia, J
last_name: Xia
- first_name: J
full_name: Hong, J
last_name: Hong
- first_name: C
full_name: Zhang, C
last_name: Zhang
- first_name: H
full_name: Wei, H
last_name: Wei
- first_name: W
full_name: Ying, W
last_name: Ying
- first_name: C
full_name: Sun, C
last_name: Sun
- first_name: L
full_name: Sun, L
last_name: Sun
- first_name: Y
full_name: Mao, Y
last_name: Mao
- first_name: Y
full_name: Gao, Y
last_name: Gao
- first_name: S
full_name: Tan, S
last_name: Tan
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: D
full_name: Li, D
last_name: Li
- first_name: X
full_name: Liu, X
last_name: Liu
- first_name: L
full_name: Sun, L
last_name: Sun
citation:
ama: Yang Z, Xia J, Hong J, et al. Structural insights into auxin recognition and
efflux by Arabidopsis PIN1. Nature. 2022;609(7927):611-615. doi:10.1038/s41586-022-05143-9
apa: Yang, Z., Xia, J., Hong, J., Zhang, C., Wei, H., Ying, W., … Sun, L. (2022).
Structural insights into auxin recognition and efflux by Arabidopsis PIN1. Nature.
Springer Nature. https://doi.org/10.1038/s41586-022-05143-9
chicago: Yang, Z, J Xia, J Hong, C Zhang, H Wei, W Ying, C Sun, et al. “Structural
Insights into Auxin Recognition and Efflux by Arabidopsis PIN1.” Nature.
Springer Nature, 2022. https://doi.org/10.1038/s41586-022-05143-9.
ieee: Z. Yang et al., “Structural insights into auxin recognition and efflux
by Arabidopsis PIN1,” Nature, vol. 609, no. 7927. Springer Nature, pp.
611–615, 2022.
ista: Yang Z, Xia J, Hong J, Zhang C, Wei H, Ying W, Sun C, Sun L, Mao Y, Gao Y,
Tan S, Friml J, Li D, Liu X, Sun L. 2022. Structural insights into auxin recognition
and efflux by Arabidopsis PIN1. Nature. 609(7927), 611–615.
mla: Yang, Z., et al. “Structural Insights into Auxin Recognition and Efflux by
Arabidopsis PIN1.” Nature, vol. 609, no. 7927, Springer Nature, 2022, pp.
611–15, doi:10.1038/s41586-022-05143-9.
short: Z. Yang, J. Xia, J. Hong, C. Zhang, H. Wei, W. Ying, C. Sun, L. Sun, Y. Mao,
Y. Gao, S. Tan, J. Friml, D. Li, X. Liu, L. Sun, Nature 609 (2022) 611–615.
date_created: 2022-09-07T14:19:52Z
date_published: 2022-08-02T00:00:00Z
date_updated: 2023-08-03T13:41:44Z
day: '02'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41586-022-05143-9
external_id:
isi:
- '000848082900002'
pmid:
- '35917925'
file:
- access_level: open_access
checksum: 3136a585f8e1c7e73b5e1418b3d01898
content_type: application/pdf
creator: dernst
date_created: 2022-09-08T08:02:54Z
date_updated: 2022-09-08T08:02:54Z
file_id: '12064'
file_name: 2022_Nature_Yang.pdf
file_size: 32344580
relation: main_file
success: 1
file_date_updated: 2022-09-08T08:02:54Z
has_accepted_license: '1'
intvolume: ' 609'
isi: 1
issue: '7927'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
page: 611-615
pmid: 1
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Structural insights into auxin recognition and efflux by Arabidopsis PIN1
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 609
year: '2022'
...
---
_id: '12121'
abstract:
- lang: eng
text: Autophagosomes are double-membraned vesicles that traffic harmful or unwanted
cellular macromolecules to the vacuole for recycling. Although autophagosome biogenesis
has been extensively studied, autophagosome maturation, i.e., delivery and fusion
with the vacuole, remains largely unknown in plants. Here, we have identified
an autophagy adaptor, CFS1, that directly interacts with the autophagosome marker
ATG8 and localizes on both membranes of the autophagosome. Autophagosomes form
normally in Arabidopsis thaliana cfs1 mutants, but their delivery to the vacuole
is disrupted. CFS1’s function is evolutionarily conserved in plants, as it also
localizes to the autophagosomes and plays a role in autophagic flux in the liverwort
Marchantia polymorpha. CFS1 regulates autophagic flux by bridging autophagosomes
with the multivesicular body-localized ESCRT-I component VPS23A, leading to the
formation of amphisomes. Similar to CFS1-ATG8 interaction, disrupting the CFS1-VPS23A
interaction blocks autophagic flux and renders plants sensitive to nitrogen starvation.
Altogether, our results reveal a conserved vacuolar sorting hub that regulates
autophagic flux in plants.
acknowledgement: "We thank Suayip Ustün, Karin Schumacher, Erika Isono, Gerd Juergens,
Takashi Ueda, Daniel Hofius, and Liwen Jiang for sharing published materials.\r\nWe
acknowledge funding from Austrian Academy of Sciences, Austrian Science Fund (FWF,
P 32355, P 34944), Austrian Science Fund (FWF-SFB F79), Vienna Science and Technology\r\nFund
(WWTF, LS17-047) to Y. Dagdas; Austrian Academy of Sciences DOC Fellowship to J.
Zhao, Marie Curie VIP2 Fellowship to J.C. De La Concepcion and M. Clavel; Hong Kong
Research Grant Council (GRF14121019, 14113921, AoE/M-05/12, C4002-17G) to B.-H.
Kang. We thank Vienna Biocenter Core Facilities (VBCF) Protein Chemistry, Biooptics,
Plant Sciences, Molecular Biology, and Protein Technologies. We thank J. Matthew
Watson\r\nand members of the Dagdas lab for the critical reading and editing of
the manuscript."
article_number: e202203139
article_processing_charge: No
article_type: original
author:
- first_name: Jierui
full_name: Zhao, Jierui
last_name: Zhao
- first_name: Mai Thu
full_name: Bui, Mai Thu
last_name: Bui
- first_name: Juncai
full_name: Ma, Juncai
last_name: Ma
- first_name: Fabian
full_name: Künzl, Fabian
last_name: Künzl
- first_name: Lorenzo
full_name: Picchianti, Lorenzo
last_name: Picchianti
- first_name: Juan Carlos
full_name: De La Concepcion, Juan Carlos
last_name: De La Concepcion
- first_name: Yixuan
full_name: Chen, Yixuan
last_name: Chen
- first_name: Sofia
full_name: Petsangouraki, Sofia
last_name: Petsangouraki
- first_name: Azadeh
full_name: Mohseni, Azadeh
last_name: Mohseni
- first_name: Marta
full_name: García-Leon, Marta
last_name: García-Leon
- first_name: Marta Salas
full_name: Gomez, Marta Salas
last_name: Gomez
- first_name: Caterina
full_name: Giannini, Caterina
id: e3fdddd5-f6e0-11ea-865d-ca99ee6367f4
last_name: Giannini
- first_name: Dubois
full_name: Gwennogan, Dubois
last_name: Gwennogan
- first_name: Roksolana
full_name: Kobylinska, Roksolana
last_name: Kobylinska
- first_name: Marion
full_name: Clavel, Marion
last_name: Clavel
- first_name: Swen
full_name: Schellmann, Swen
last_name: Schellmann
- first_name: Yvon
full_name: Jaillais, Yvon
last_name: Jaillais
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Byung-Ho
full_name: Kang, Byung-Ho
last_name: Kang
- first_name: Yasin
full_name: Dagdas, Yasin
last_name: Dagdas
citation:
ama: Zhao J, Bui MT, Ma J, et al. Plant autophagosomes mature into amphisomes prior
to their delivery to the central vacuole. Journal of Cell Biology. 2022;221(12).
doi:10.1083/jcb.202203139
apa: Zhao, J., Bui, M. T., Ma, J., Künzl, F., Picchianti, L., De La Concepcion,
J. C., … Dagdas, Y. (2022). Plant autophagosomes mature into amphisomes prior
to their delivery to the central vacuole. Journal of Cell Biology. Rockefeller
University Press. https://doi.org/10.1083/jcb.202203139
chicago: Zhao, Jierui, Mai Thu Bui, Juncai Ma, Fabian Künzl, Lorenzo Picchianti,
Juan Carlos De La Concepcion, Yixuan Chen, et al. “Plant Autophagosomes Mature
into Amphisomes Prior to Their Delivery to the Central Vacuole.” Journal of
Cell Biology. Rockefeller University Press, 2022. https://doi.org/10.1083/jcb.202203139.
ieee: J. Zhao et al., “Plant autophagosomes mature into amphisomes prior
to their delivery to the central vacuole,” Journal of Cell Biology, vol.
221, no. 12. Rockefeller University Press, 2022.
ista: Zhao J, Bui MT, Ma J, Künzl F, Picchianti L, De La Concepcion JC, Chen Y,
Petsangouraki S, Mohseni A, García-Leon M, Gomez MS, Giannini C, Gwennogan D,
Kobylinska R, Clavel M, Schellmann S, Jaillais Y, Friml J, Kang B-H, Dagdas Y.
2022. Plant autophagosomes mature into amphisomes prior to their delivery to the
central vacuole. Journal of Cell Biology. 221(12), e202203139.
mla: Zhao, Jierui, et al. “Plant Autophagosomes Mature into Amphisomes Prior to
Their Delivery to the Central Vacuole.” Journal of Cell Biology, vol. 221,
no. 12, e202203139, Rockefeller University Press, 2022, doi:10.1083/jcb.202203139.
short: J. Zhao, M.T. Bui, J. Ma, F. Künzl, L. Picchianti, J.C. De La Concepcion,
Y. Chen, S. Petsangouraki, A. Mohseni, M. García-Leon, M.S. Gomez, C. Giannini,
D. Gwennogan, R. Kobylinska, M. Clavel, S. Schellmann, Y. Jaillais, J. Friml,
B.-H. Kang, Y. Dagdas, Journal of Cell Biology 221 (2022).
date_created: 2023-01-12T11:57:10Z
date_published: 2022-12-01T00:00:00Z
date_updated: 2023-08-03T14:20:15Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1083/jcb.202203139
external_id:
isi:
- '000932958800001'
pmid:
- '36260289'
file:
- access_level: open_access
checksum: 050b5cc4b25e6b94fe3e3cbfe0f5c06b
content_type: application/pdf
creator: dernst
date_created: 2023-01-23T10:30:11Z
date_updated: 2023-01-23T10:30:11Z
file_id: '12342'
file_name: 2022_JCB_Zhao.pdf
file_size: 10365777
relation: main_file
success: 1
file_date_updated: 2023-01-23T10:30:11Z
has_accepted_license: '1'
intvolume: ' 221'
isi: 1
issue: '12'
keyword:
- Cell Biology
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
pmid: 1
publication: Journal of Cell Biology
publication_identifier:
eissn:
- 1540-8140
issn:
- 0021-9525
publication_status: published
publisher: Rockefeller University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Plant autophagosomes mature into amphisomes prior to their delivery to the
central vacuole
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 221
year: '2022'
...