---
_id: '1562'
abstract:
- lang: eng
text: The plant hormone auxin is a key regulator of plant growth and development.
Auxin levels are sensed and interpreted by distinct receptor systems that activate
a broad range of cellular responses. The Auxin-Binding Protein1 (ABP1) that has
been identified based on its ability to bind auxin with high affinity is a prime
candidate for the extracellular receptor responsible for mediating a range of
auxin effects, in particular, the fast non-transcriptional ones. Contradictory
genetic studies suggested prominent or no importance of ABP1 in many developmental
processes. However, how crucial the role of auxin binding to ABP1 is for its functions
has not been addressed. Here, we show that the auxin-binding pocket of ABP1 is
essential for its gain-of-function cellular and developmental roles. In total,
16 different abp1 mutants were prepared that possessed substitutions in the metal
core or in the hydrophobic amino acids of the auxin-binding pocket as well as
neutral mutations. Their analysis revealed that an intact auxin-binding pocket
is a prerequisite for ABP1 to activate downstream components of the ABP1 signalling
pathway, such as Rho of Plants (ROPs) and to mediate the clathrin association
with membranes for endocytosis regulation. In planta analyses demonstrated the
importance of the auxin binding pocket for all known ABP1-mediated postembryonic
developmental processes, including morphology of leaf epidermal cells, root growth
and root meristem activity, and vascular tissue differentiation. Taken together,
these findings suggest that auxin binding to ABP1 is central to its function,
supporting the role of ABP1 as auxin receptor.
acknowledgement: This work was supported by ERC Independent Research grant (ERC-2011-StG-
20101109-PSDP to JF); the European Social Fund and the state budget of the Czech
Republic [the project ‘Employment of Newly Graduated Doctors of Science for Scientific
Excellence’ (CZ.1.07/2.3.00/30.0009) to TN]; the Czech Science Foundation (GACR)
[project 13-40637S to JF].
article_type: original
author:
- first_name: Peter
full_name: Grones, Peter
id: 399876EC-F248-11E8-B48F-1D18A9856A87
last_name: Grones
- first_name: Xu
full_name: Chen, Xu
id: 4E5ADCAA-F248-11E8-B48F-1D18A9856A87
last_name: Chen
- first_name: Sibu
full_name: Simon, Sibu
id: 4542EF9A-F248-11E8-B48F-1D18A9856A87
last_name: Simon
orcid: 0000-0002-1998-6741
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Riet
full_name: De Rycke, Riet
last_name: De Rycke
- first_name: Tomasz
full_name: Nodzyński, Tomasz
last_name: Nodzyński
- first_name: Eva
full_name: Zažímalová, Eva
last_name: Zažímalová
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Grones P, Chen X, Simon S, et al. Auxin-binding pocket of ABP1 is crucial for
its gain-of-function cellular and developmental roles. Journal of Experimental
Botany. 2015;66(16):5055-5065. doi:10.1093/jxb/erv177
apa: Grones, P., Chen, X., Simon, S., Kaufmann, W., De Rycke, R., Nodzyński, T.,
… Friml, J. (2015). Auxin-binding pocket of ABP1 is crucial for its gain-of-function
cellular and developmental roles. Journal of Experimental Botany. Oxford
University Press. https://doi.org/10.1093/jxb/erv177
chicago: Grones, Peter, Xu Chen, Sibu Simon, Walter Kaufmann, Riet De Rycke, Tomasz
Nodzyński, Eva Zažímalová, and Jiří Friml. “Auxin-Binding Pocket of ABP1 Is Crucial
for Its Gain-of-Function Cellular and Developmental Roles.” Journal of Experimental
Botany. Oxford University Press, 2015. https://doi.org/10.1093/jxb/erv177.
ieee: P. Grones et al., “Auxin-binding pocket of ABP1 is crucial for its
gain-of-function cellular and developmental roles,” Journal of Experimental
Botany, vol. 66, no. 16. Oxford University Press, pp. 5055–5065, 2015.
ista: Grones P, Chen X, Simon S, Kaufmann W, De Rycke R, Nodzyński T, Zažímalová
E, Friml J. 2015. Auxin-binding pocket of ABP1 is crucial for its gain-of-function
cellular and developmental roles. Journal of Experimental Botany. 66(16), 5055–5065.
mla: Grones, Peter, et al. “Auxin-Binding Pocket of ABP1 Is Crucial for Its Gain-of-Function
Cellular and Developmental Roles.” Journal of Experimental Botany, vol.
66, no. 16, Oxford University Press, 2015, pp. 5055–65, doi:10.1093/jxb/erv177.
short: P. Grones, X. Chen, S. Simon, W. Kaufmann, R. De Rycke, T. Nodzyński, E.
Zažímalová, J. Friml, Journal of Experimental Botany 66 (2015) 5055–5065.
date_created: 2018-12-11T11:52:44Z
date_published: 2015-08-01T00:00:00Z
date_updated: 2023-02-23T10:04:26Z
day: '01'
department:
- _id: JiFr
- _id: EM-Fac
doi: 10.1093/jxb/erv177
ec_funded: 1
intvolume: ' 66'
issue: '16'
language:
- iso: eng
month: '08'
oa_version: None
page: 5055 - 5065
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Journal of Experimental Botany
publication_status: published
publisher: Oxford University Press
publist_id: '5609'
quality_controlled: '1'
scopus_import: 1
status: public
title: Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and
developmental roles
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 66
year: '2015'
...
---
_id: '1574'
abstract:
- lang: eng
text: Multiple plant developmental processes, such as lateral root development,
depend on auxin distribution patterns that are in part generated by the PIN-formed
family of auxin-efflux transporters. Here we propose that AUXIN RESPONSE FACTOR7
(ARF7) and the ARF7-regulated FOUR LIPS/MYB124 (FLP) transcription factors jointly
form a coherent feed-forward motif that mediates the auxin-responsive PIN3 transcription
in planta to steer the early steps of lateral root formation. This regulatory
mechanism might endow the PIN3 circuitry with a temporal 'memory' of auxin stimuli,
potentially maintaining and enhancing the robustness of the auxin flux directionality
during lateral root development. The cooperative action between canonical auxin
signalling and other transcription factors might constitute a general mechanism
by which transcriptional auxin-sensitivity can be regulated at a tissue-specific
level.
acknowledgement: 'of the European Research Council (project ERC-2011-StG-20101109-PSDP)
(to J.F.), a FEBS long-term fellowship (to P.M.) '
article_number: '8821'
author:
- first_name: Qian
full_name: Chen, Qian
last_name: Chen
- first_name: Yang
full_name: Liu, Yang
last_name: Liu
- first_name: Steven
full_name: Maere, Steven
last_name: Maere
- first_name: Eunkyoung
full_name: Lee, Eunkyoung
last_name: Lee
- first_name: Gert
full_name: Van Isterdael, Gert
last_name: Van Isterdael
- first_name: Zidian
full_name: Xie, Zidian
last_name: Xie
- first_name: Wei
full_name: Xuan, Wei
last_name: Xuan
- first_name: Jessica
full_name: Lucas, Jessica
last_name: Lucas
- first_name: Valya
full_name: Vassileva, Valya
last_name: Vassileva
- first_name: Saeko
full_name: Kitakura, Saeko
last_name: Kitakura
- first_name: Peter
full_name: Marhavy, Peter
id: 3F45B078-F248-11E8-B48F-1D18A9856A87
last_name: Marhavy
orcid: 0000-0001-5227-5741
- first_name: Krzysztof T
full_name: Wabnik, Krzysztof T
id: 4DE369A4-F248-11E8-B48F-1D18A9856A87
last_name: Wabnik
orcid: 0000-0001-7263-0560
- first_name: Niko
full_name: Geldner, Niko
last_name: Geldner
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Jie
full_name: Le, Jie
last_name: Le
- first_name: Hidehiro
full_name: Fukaki, Hidehiro
last_name: Fukaki
- first_name: Erich
full_name: Grotewold, Erich
last_name: Grotewold
- first_name: Chuanyou
full_name: Li, Chuanyou
last_name: Li
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Fred
full_name: Sack, Fred
last_name: Sack
- first_name: Tom
full_name: Beeckman, Tom
last_name: Beeckman
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
citation:
ama: Chen Q, Liu Y, Maere S, et al. A coherent transcriptional feed-forward motif
model for mediating auxin-sensitive PIN3 expression during lateral root development.
Nature Communications. 2015;6. doi:10.1038/ncomms9821
apa: Chen, Q., Liu, Y., Maere, S., Lee, E., Van Isterdael, G., Xie, Z., … Vanneste,
S. (2015). A coherent transcriptional feed-forward motif model for mediating auxin-sensitive
PIN3 expression during lateral root development. Nature Communications.
Nature Publishing Group. https://doi.org/10.1038/ncomms9821
chicago: Chen, Qian, Yang Liu, Steven Maere, Eunkyoung Lee, Gert Van Isterdael,
Zidian Xie, Wei Xuan, et al. “A Coherent Transcriptional Feed-Forward Motif Model
for Mediating Auxin-Sensitive PIN3 Expression during Lateral Root Development.”
Nature Communications. Nature Publishing Group, 2015. https://doi.org/10.1038/ncomms9821.
ieee: Q. Chen et al., “A coherent transcriptional feed-forward motif model
for mediating auxin-sensitive PIN3 expression during lateral root development,”
Nature Communications, vol. 6. Nature Publishing Group, 2015.
ista: Chen Q, Liu Y, Maere S, Lee E, Van Isterdael G, Xie Z, Xuan W, Lucas J, Vassileva
V, Kitakura S, Marhavý P, Wabnik KT, Geldner N, Benková E, Le J, Fukaki H, Grotewold
E, Li C, Friml J, Sack F, Beeckman T, Vanneste S. 2015. A coherent transcriptional
feed-forward motif model for mediating auxin-sensitive PIN3 expression during
lateral root development. Nature Communications. 6, 8821.
mla: Chen, Qian, et al. “A Coherent Transcriptional Feed-Forward Motif Model for
Mediating Auxin-Sensitive PIN3 Expression during Lateral Root Development.” Nature
Communications, vol. 6, 8821, Nature Publishing Group, 2015, doi:10.1038/ncomms9821.
short: Q. Chen, Y. Liu, S. Maere, E. Lee, G. Van Isterdael, Z. Xie, W. Xuan, J.
Lucas, V. Vassileva, S. Kitakura, P. Marhavý, K.T. Wabnik, N. Geldner, E. Benková,
J. Le, H. Fukaki, E. Grotewold, C. Li, J. Friml, F. Sack, T. Beeckman, S. Vanneste,
Nature Communications 6 (2015).
date_created: 2018-12-11T11:52:48Z
date_published: 2015-11-18T00:00:00Z
date_updated: 2021-01-12T06:51:42Z
day: '18'
ddc:
- '580'
department:
- _id: EvBe
- _id: JiFr
doi: 10.1038/ncomms9821
file:
- access_level: open_access
checksum: 8ff5c108899b548806e1cb7a302fe76d
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:14:32Z
date_updated: 2020-07-14T12:45:02Z
file_id: '5085'
file_name: IST-2016-477-v1+1_ncomms9821.pdf
file_size: 1701815
relation: main_file
file_date_updated: 2020-07-14T12:45:02Z
has_accepted_license: '1'
intvolume: ' 6'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
publication: Nature Communications
publication_status: published
publisher: Nature Publishing Group
publist_id: '5597'
pubrep_id: '477'
quality_controlled: '1'
scopus_import: 1
status: public
title: A coherent transcriptional feed-forward motif model for mediating auxin-sensitive
PIN3 expression during lateral root development
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 6
year: '2015'
...
---
_id: '1569'
abstract:
- lang: eng
text: Spatial regulation of the plant hormone indole-3-acetic acid (IAA, or auxin)
is essential for plant development. Auxin gradient establishment is mediated by
polarly localized auxin transporters, including PIN-FORMED (PIN) proteins. Their
localization and abundance at the plasma membrane are tightly regulated by endomembrane
machinery, especially the endocytic and recycling pathways mediated by the ADP
ribosylation factor guanine nucleotide exchange factor (ARF-GEF) GNOM. We assessed
the role of the early secretory pathway in establishing PIN1 polarity in Arabidopsis
thaliana by pharmacological and genetic approaches. We identified the compound
endosidin 8 (ES8), which selectively interferes with PIN1 basal polarity without
altering the polarity of apical proteins. ES8 alters the auxin distribution pattern
in the root and induces a strong developmental phenotype, including reduced root
length. The ARF-GEF- defective mutants gnom-like 1 ( gnl1-1) and gnom ( van7)
are significantly resistant to ES8. The compound does not affect recycling or
vacuolar trafficking of PIN1 but leads to its intracellular accumulation, resulting
in loss of PIN1 basal polarity at the plasma membrane. Our data confirm a role
for GNOM in endoplasmic reticulum (ER) - Golgi trafficking and reveal that a GNL1/GNOM-mediated
early secretory pathway selectively regulates PIN1 basal polarity establishment
in a manner essential for normal plant development.
acknowledgement: 'This work was supported by Vetenskapsrådet and Vinnova (Verket för
Innovationssystemet) (S.M.D., T.V., M.Ł., and S.R.), Knut och Alice Wallenbergs
Stiftelse (S.M.D., A.R., and C.V.), Kempestiftelserna (A.H. and Q.M.), Carl Tryggers
Stiftelse för Vetenskaplig Forskning (Q.M.), European Research Council Grant ERC-2011-StG-20101109-PSDP
(to J.F.), US Department of Energy Grant DE-FG02-02ER15295 (to N.V.R.), and National
Science Foundation Grant MCB-0817916 (to N.V.R. and G.R.H.). '
author:
- first_name: Siamsa
full_name: Doyle, Siamsa
last_name: Doyle
- first_name: Ash
full_name: Haegera, Ash
last_name: Haegera
- first_name: Thomas
full_name: Vain, Thomas
last_name: Vain
- first_name: Adeline
full_name: Rigala, Adeline
last_name: Rigala
- first_name: Corrado
full_name: Viotti, Corrado
last_name: Viotti
- first_name: Małgorzata
full_name: Łangowskaa, Małgorzata
last_name: Łangowskaa
- first_name: Qian
full_name: Maa, Qian
last_name: Maa
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Natasha
full_name: Raikhel, Natasha
last_name: Raikhel
- first_name: Glenn
full_name: Hickse, Glenn
last_name: Hickse
- first_name: Stéphanie
full_name: Robert, Stéphanie
last_name: Robert
citation:
ama: Doyle S, Haegera A, Vain T, et al. An early secretory pathway mediated by gnom-like
1 and gnom is essential for basal polarity establishment in Arabidopsis thaliana.
PNAS. 2015;112(7):E806-E815. doi:10.1073/pnas.1424856112
apa: Doyle, S., Haegera, A., Vain, T., Rigala, A., Viotti, C., Łangowskaa, M., …
Robert, S. (2015). An early secretory pathway mediated by gnom-like 1 and gnom
is essential for basal polarity establishment in Arabidopsis thaliana. PNAS.
National Academy of Sciences. https://doi.org/10.1073/pnas.1424856112
chicago: Doyle, Siamsa, Ash Haegera, Thomas Vain, Adeline Rigala, Corrado Viotti,
Małgorzata Łangowskaa, Qian Maa, et al. “An Early Secretory Pathway Mediated by
Gnom-like 1 and Gnom Is Essential for Basal Polarity Establishment in Arabidopsis
Thaliana.” PNAS. National Academy of Sciences, 2015. https://doi.org/10.1073/pnas.1424856112.
ieee: S. Doyle et al., “An early secretory pathway mediated by gnom-like
1 and gnom is essential for basal polarity establishment in Arabidopsis thaliana,”
PNAS, vol. 112, no. 7. National Academy of Sciences, pp. E806–E815, 2015.
ista: Doyle S, Haegera A, Vain T, Rigala A, Viotti C, Łangowskaa M, Maa Q, Friml
J, Raikhel N, Hickse G, Robert S. 2015. An early secretory pathway mediated by
gnom-like 1 and gnom is essential for basal polarity establishment in Arabidopsis
thaliana. PNAS. 112(7), E806–E815.
mla: Doyle, Siamsa, et al. “An Early Secretory Pathway Mediated by Gnom-like 1 and
Gnom Is Essential for Basal Polarity Establishment in Arabidopsis Thaliana.” PNAS,
vol. 112, no. 7, National Academy of Sciences, 2015, pp. E806–15, doi:10.1073/pnas.1424856112.
short: S. Doyle, A. Haegera, T. Vain, A. Rigala, C. Viotti, M. Łangowskaa, Q. Maa,
J. Friml, N. Raikhel, G. Hickse, S. Robert, PNAS 112 (2015) E806–E815.
date_created: 2018-12-11T11:52:46Z
date_published: 2015-02-17T00:00:00Z
date_updated: 2021-01-12T06:51:39Z
day: '17'
department:
- _id: JiFr
doi: 10.1073/pnas.1424856112
ec_funded: 1
intvolume: ' 112'
issue: '7'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4343110/
month: '02'
oa: 1
oa_version: Published Version
page: E806 - E815
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '5602'
quality_controlled: '1'
scopus_import: 1
status: public
title: An early secretory pathway mediated by gnom-like 1 and gnom is essential for
basal polarity establishment in Arabidopsis thaliana
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 112
year: '2015'
...
---
_id: '1640'
abstract:
- lang: eng
text: Auxin and cytokinin are key endogenous regulators of plant development. Although
cytokinin-mediated modulation of auxin distribution is a developmentally crucial
hormonal interaction, its molecular basis is largely unknown. Here we show a direct
regulatory link between cytokinin signalling and the auxin transport machinery
uncovering a mechanistic framework for cytokinin-auxin cross-talk. We show that
the CYTOKININ RESPONSE FACTORS (CRFs), transcription factors downstream of cytokinin
perception, transcriptionally control genes encoding PIN-FORMED (PIN) auxin transporters
at a specific PIN CYTOKININ RESPONSE ELEMENT (PCRE) domain. Removal of this cis-regulatory
element effectively uncouples PIN transcription from the CRF-mediated cytokinin
regulation and attenuates plant cytokinin sensitivity. We propose that CRFs represent
a missing cross-talk component that fine-tunes auxin transport capacity downstream
of cytokinin signalling to control plant development.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: This work was supported by the European Research Council Starting
Independent Research grant (ERC-2007-Stg-207362-HCPO to E.B., M.S., C.C.), by the
Ghent University Multidisciplinary Research Partnership ‘Biotechnology for a Sustainable
Economy’ no.01MRB510W, by the Research Foundation—Flanders (grant 3G033711 to J.-A.O.),
by the Austrian Science Fund (FWF01_I1774S) to K.Ö.,E.B., and by the Interuniversity
Attraction Poles Programme (IUAP P7/29 ‘MARS’) initiated by the Belgian Science
Policy Office. I.D.C. and S.V. are post-doctoral fellows of the Research Foundation—Flanders
(FWO). This research was supported by the Scientific Service Units (SSU) of IST-Austria
through resources provided by the Bioimaging Facility (BIF), the Life Science Facility
(LSF).
article_number: '8717'
author:
- first_name: Mária
full_name: Šimášková, Mária
last_name: Šimášková
- first_name: José
full_name: O'Brien, José
last_name: O'Brien
- first_name: Mamoona
full_name: Khan-Djamei, Mamoona
id: 391B5BBC-F248-11E8-B48F-1D18A9856A87
last_name: Khan-Djamei
- first_name: Giel
full_name: Van Noorden, Giel
last_name: Van Noorden
- first_name: Krisztina
full_name: Ötvös, Krisztina
id: 29B901B0-F248-11E8-B48F-1D18A9856A87
last_name: Ötvös
orcid: 0000-0002-5503-4983
- first_name: Anne
full_name: Vieten, Anne
last_name: Vieten
- first_name: Inge
full_name: De Clercq, Inge
last_name: De Clercq
- first_name: Johanna
full_name: Van Haperen, Johanna
last_name: Van Haperen
- first_name: Candela
full_name: Cuesta, Candela
id: 33A3C818-F248-11E8-B48F-1D18A9856A87
last_name: Cuesta
orcid: 0000-0003-1923-2410
- first_name: Klára
full_name: Hoyerová, Klára
last_name: Hoyerová
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Peter
full_name: Marhavy, Peter
id: 3F45B078-F248-11E8-B48F-1D18A9856A87
last_name: Marhavy
orcid: 0000-0001-5227-5741
- first_name: Krzysztof T
full_name: Wabnik, Krzysztof T
id: 4DE369A4-F248-11E8-B48F-1D18A9856A87
last_name: Wabnik
orcid: 0000-0001-7263-0560
- first_name: Frank
full_name: Van Breusegem, Frank
last_name: Van Breusegem
- first_name: Moritz
full_name: Nowack, Moritz
last_name: Nowack
- first_name: Angus
full_name: Murphy, Angus
last_name: Murphy
- first_name: Jiřĺ
full_name: Friml, Jiřĺ
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
- first_name: Tom
full_name: Beeckman, Tom
last_name: Beeckman
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Šimášková M, O’Brien J, Khan-Djamei M, et al. Cytokinin response factors regulate
PIN-FORMED auxin transporters. Nature Communications. 2015;6. doi:10.1038/ncomms9717
apa: Šimášková, M., O’Brien, J., Khan-Djamei, M., Van Noorden, G., Ötvös, K., Vieten,
A., … Benková, E. (2015). Cytokinin response factors regulate PIN-FORMED auxin
transporters. Nature Communications. Nature Publishing Group. https://doi.org/10.1038/ncomms9717
chicago: Šimášková, Mária, José O’Brien, Mamoona Khan-Djamei, Giel Van Noorden,
Krisztina Ötvös, Anne Vieten, Inge De Clercq, et al. “Cytokinin Response Factors
Regulate PIN-FORMED Auxin Transporters.” Nature Communications. Nature
Publishing Group, 2015. https://doi.org/10.1038/ncomms9717.
ieee: M. Šimášková et al., “Cytokinin response factors regulate PIN-FORMED
auxin transporters,” Nature Communications, vol. 6. Nature Publishing Group,
2015.
ista: Šimášková M, O’Brien J, Khan-Djamei M, Van Noorden G, Ötvös K, Vieten A, De
Clercq I, Van Haperen J, Cuesta C, Hoyerová K, Vanneste S, Marhavý P, Wabnik KT,
Van Breusegem F, Nowack M, Murphy A, Friml J, Weijers D, Beeckman T, Benková E.
2015. Cytokinin response factors regulate PIN-FORMED auxin transporters. Nature
Communications. 6, 8717.
mla: Šimášková, Mária, et al. “Cytokinin Response Factors Regulate PIN-FORMED Auxin
Transporters.” Nature Communications, vol. 6, 8717, Nature Publishing Group,
2015, doi:10.1038/ncomms9717.
short: M. Šimášková, J. O’Brien, M. Khan-Djamei, G. Van Noorden, K. Ötvös, A. Vieten,
I. De Clercq, J. Van Haperen, C. Cuesta, K. Hoyerová, S. Vanneste, P. Marhavý,
K.T. Wabnik, F. Van Breusegem, M. Nowack, A. Murphy, J. Friml, D. Weijers, T.
Beeckman, E. Benková, Nature Communications 6 (2015).
date_created: 2018-12-11T11:53:12Z
date_published: 2015-01-01T00:00:00Z
date_updated: 2021-01-12T06:52:11Z
day: '01'
ddc:
- '580'
department:
- _id: EvBe
- _id: JiFr
doi: 10.1038/ncomms9717
ec_funded: 1
file:
- access_level: open_access
checksum: c2c84bca37401435fedf76bad0ba0579
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:18:36Z
date_updated: 2020-07-14T12:45:08Z
file_id: '5358'
file_name: IST-2018-1020-v1+1_Simaskova_et_al_NatCom_2015.pdf
file_size: 1471217
relation: main_file
file_date_updated: 2020-07-14T12:45:08Z
has_accepted_license: '1'
intvolume: ' 6'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Submitted Version
project:
- _id: 253FCA6A-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '207362'
name: Hormonal cross-talk in plant organogenesis
- _id: 2542D156-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I 1774-B16
name: Hormone cross-talk drives nutrient dependent plant development
publication: Nature Communications
publication_status: published
publisher: Nature Publishing Group
publist_id: '5513'
pubrep_id: '1020'
quality_controlled: '1'
scopus_import: 1
status: public
title: Cytokinin response factors regulate PIN-FORMED auxin transporters
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 6
year: '2015'
...
---
_id: '1819'
abstract:
- lang: eng
text: 'The sessile life style of plants creates the need to deal with an often adverse
environment, in which water availability can change on a daily basis, challenging
the cellular physiology and integrity. Changes in osmotic conditions disrupt the
equilibrium of the plasma membrane: hypoosmotic conditions increase and hyperosmotic
environment decrease the cell volume. Here, we show that short-term extracellular
osmotic treatments are closely followed by a shift in the balance between endocytosis
and exocytosis in root meristem cells. Acute hyperosmotic treatments (ionic and
nonionic) enhance clathrin-mediated endocytosis simultaneously attenuating exocytosis,
whereas hypoosmotic treatments have the opposite effects. In addition to clathrin
recruitment to the plasma membrane, components of early endocytic trafficking
are essential during hyperosmotic stress responses. Consequently, growth of seedlings
defective in elements of clathrin or early endocytic machinery is more sensitive
to hyperosmotic treatments. We also found that the endocytotic response to a change
of osmotic status in the environment is dominant over the presumably evolutionary
more recent regulatory effect of plant hormones, such as auxin. These results
imply that osmotic perturbation influences the balance between endocytosis and
exocytosis acting through clathrin-mediated endocytosis. We propose that tension
on the plasma membrane determines the addition or removal of membranes at the
cell surface, thus preserving cell integrity.'
acknowledgement: This work was supported by the European Research Council (project
ERC-2011-StG-20101109-PSDP); European Social Fund (CZ.1.07/2.3.00/20.0043) and the
Czech Science Foundation GAČR (GA13-40637S) to J.F.; project Postdoc I. (CZ.1.07/2.3.00/30.0009)
co-financed by the European Social Fund and the state budget of the Czech Republic
to M.Z. and T.N..
author:
- first_name: Marta
full_name: Zwiewka, Marta
last_name: Zwiewka
- first_name: Tomasz
full_name: Nodzyński, Tomasz
last_name: Nodzyński
- first_name: Stéphanie
full_name: Robert, Stéphanie
last_name: Robert
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Jiřĺ
full_name: Friml, Jiřĺ
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Zwiewka M, Nodzyński T, Robert S, Vanneste S, Friml J. Osmotic stress modulates
the balance between exocytosis and clathrin mediated endocytosis in Arabidopsis
thaliana. Molecular Plant. 2015;8(8):1175-1187. doi:10.1016/j.molp.2015.03.007
apa: Zwiewka, M., Nodzyński, T., Robert, S., Vanneste, S., & Friml, J. (2015).
Osmotic stress modulates the balance between exocytosis and clathrin mediated
endocytosis in Arabidopsis thaliana. Molecular Plant. Elsevier. https://doi.org/10.1016/j.molp.2015.03.007
chicago: Zwiewka, Marta, Tomasz Nodzyński, Stéphanie Robert, Steffen Vanneste, and
Jiří Friml. “Osmotic Stress Modulates the Balance between Exocytosis and Clathrin
Mediated Endocytosis in Arabidopsis Thaliana.” Molecular Plant. Elsevier,
2015. https://doi.org/10.1016/j.molp.2015.03.007.
ieee: M. Zwiewka, T. Nodzyński, S. Robert, S. Vanneste, and J. Friml, “Osmotic stress
modulates the balance between exocytosis and clathrin mediated endocytosis in
Arabidopsis thaliana,” Molecular Plant, vol. 8, no. 8. Elsevier, pp. 1175–1187,
2015.
ista: Zwiewka M, Nodzyński T, Robert S, Vanneste S, Friml J. 2015. Osmotic stress
modulates the balance between exocytosis and clathrin mediated endocytosis in
Arabidopsis thaliana. Molecular Plant. 8(8), 1175–1187.
mla: Zwiewka, Marta, et al. “Osmotic Stress Modulates the Balance between Exocytosis
and Clathrin Mediated Endocytosis in Arabidopsis Thaliana.” Molecular Plant,
vol. 8, no. 8, Elsevier, 2015, pp. 1175–87, doi:10.1016/j.molp.2015.03.007.
short: M. Zwiewka, T. Nodzyński, S. Robert, S. Vanneste, J. Friml, Molecular Plant
8 (2015) 1175–1187.
date_created: 2018-12-11T11:54:11Z
date_published: 2015-08-03T00:00:00Z
date_updated: 2021-01-12T06:53:24Z
day: '03'
department:
- _id: JiFr
doi: 10.1016/j.molp.2015.03.007
ec_funded: 1
intvolume: ' 8'
issue: '8'
language:
- iso: eng
month: '08'
oa_version: None
page: 1175 - 1187
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Molecular Plant
publication_status: published
publisher: Elsevier
publist_id: '5287'
quality_controlled: '1'
scopus_import: 1
status: public
title: Osmotic stress modulates the balance between exocytosis and clathrin mediated
endocytosis in Arabidopsis thaliana
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 8
year: '2015'
...
---
_id: '1849'
abstract:
- lang: eng
text: 'Cell polarity is a fundamental property of pro- and eukaryotic cells. It
is necessary for coordination of cell division, cell morphogenesis and signaling
processes. How polarity is generated and maintained is a complex issue governed
by interconnected feed-back regulations between small GTPase signaling and membrane
tension-based signaling that controls membrane trafficking, and cytoskeleton organization
and dynamics. Here, we will review the potential role for calcium as a crucial
signal that connects and coordinates the respective processes during polarization
processes in plants. This article is part of a Special Issue entitled: 13th European
Symposium on Calcium.'
acknowledgement: The contributing authors were supported by the Ghent University Special
Research Fund (to E.H.), the Interuniversity Attraction Poles Programme (IAP VI/33
and IUAP P7/29 ‘MARS’), the European Research Council (project ERC-2011-StG-20101109-PSDP,
to J.F.), and the Research Foundation Flanders (to S.V.).
author:
- first_name: Ellie
full_name: Himschoot, Ellie
last_name: Himschoot
- first_name: Tom
full_name: Beeckman, Tom
last_name: Beeckman
- first_name: Jiřĺ
full_name: Friml, Jiřĺ
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
citation:
ama: Himschoot E, Beeckman T, Friml J, Vanneste S. Calcium is an organizer of cell
polarity in plants. Biochimica et Biophysica Acta - Molecular Cell Research.
2015;1853(9):2168-2172. doi:10.1016/j.bbamcr.2015.02.017
apa: Himschoot, E., Beeckman, T., Friml, J., & Vanneste, S. (2015). Calcium
is an organizer of cell polarity in plants. Biochimica et Biophysica Acta -
Molecular Cell Research. Elsevier. https://doi.org/10.1016/j.bbamcr.2015.02.017
chicago: Himschoot, Ellie, Tom Beeckman, Jiří Friml, and Steffen Vanneste. “Calcium
Is an Organizer of Cell Polarity in Plants.” Biochimica et Biophysica Acta
- Molecular Cell Research. Elsevier, 2015. https://doi.org/10.1016/j.bbamcr.2015.02.017.
ieee: E. Himschoot, T. Beeckman, J. Friml, and S. Vanneste, “Calcium is an organizer
of cell polarity in plants,” Biochimica et Biophysica Acta - Molecular Cell
Research, vol. 1853, no. 9. Elsevier, pp. 2168–2172, 2015.
ista: Himschoot E, Beeckman T, Friml J, Vanneste S. 2015. Calcium is an organizer
of cell polarity in plants. Biochimica et Biophysica Acta - Molecular Cell Research.
1853(9), 2168–2172.
mla: Himschoot, Ellie, et al. “Calcium Is an Organizer of Cell Polarity in Plants.”
Biochimica et Biophysica Acta - Molecular Cell Research, vol. 1853, no.
9, Elsevier, 2015, pp. 2168–72, doi:10.1016/j.bbamcr.2015.02.017.
short: E. Himschoot, T. Beeckman, J. Friml, S. Vanneste, Biochimica et Biophysica
Acta - Molecular Cell Research 1853 (2015) 2168–2172.
date_created: 2018-12-11T11:54:21Z
date_published: 2015-09-01T00:00:00Z
date_updated: 2021-01-12T06:53:36Z
day: '01'
department:
- _id: JiFr
doi: 10.1016/j.bbamcr.2015.02.017
intvolume: ' 1853'
issue: '9'
language:
- iso: eng
month: '09'
oa_version: None
page: 2168 - 2172
publication: Biochimica et Biophysica Acta - Molecular Cell Research
publication_status: published
publisher: Elsevier
publist_id: '5252'
quality_controlled: '1'
scopus_import: 1
status: public
title: Calcium is an organizer of cell polarity in plants
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 1853
year: '2015'
...
---
_id: '1847'
acknowledgement: This work was supported by the European Research Council (project
ERC-2011-StG-20101109-PSDP), European Social Fund (CZ.1.07/2.3.00/20.0043), and
the Czech Science Foundation GAČR (GA13-40637S).
author:
- first_name: Peter
full_name: Grones, Peter
id: 399876EC-F248-11E8-B48F-1D18A9856A87
last_name: Grones
- first_name: Jiřĺ
full_name: Friml, Jiřĺ
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Grones P, Friml J. ABP1: Finally docking. Molecular Plant. 2015;8(3):356-358.
doi:10.1016/j.molp.2014.12.013'
apa: 'Grones, P., & Friml, J. (2015). ABP1: Finally docking. Molecular Plant.
Elsevier. https://doi.org/10.1016/j.molp.2014.12.013'
chicago: 'Grones, Peter, and Jiří Friml. “ABP1: Finally Docking.” Molecular Plant.
Elsevier, 2015. https://doi.org/10.1016/j.molp.2014.12.013.'
ieee: 'P. Grones and J. Friml, “ABP1: Finally docking,” Molecular Plant,
vol. 8, no. 3. Elsevier, pp. 356–358, 2015.'
ista: 'Grones P, Friml J. 2015. ABP1: Finally docking. Molecular Plant. 8(3), 356–358.'
mla: 'Grones, Peter, and Jiří Friml. “ABP1: Finally Docking.” Molecular Plant,
vol. 8, no. 3, Elsevier, 2015, pp. 356–58, doi:10.1016/j.molp.2014.12.013.'
short: P. Grones, J. Friml, Molecular Plant 8 (2015) 356–358.
date_created: 2018-12-11T11:54:20Z
date_published: 2015-03-02T00:00:00Z
date_updated: 2021-01-12T06:53:35Z
day: '02'
department:
- _id: JiFr
doi: 10.1016/j.molp.2014.12.013
intvolume: ' 8'
issue: '3'
language:
- iso: eng
month: '03'
oa_version: None
page: 356 - 358
publication: Molecular Plant
publication_status: published
publisher: Elsevier
publist_id: '5254'
quality_controlled: '1'
scopus_import: 1
status: public
title: 'ABP1: Finally docking'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 8
year: '2015'
...
---
_id: '1865'
abstract:
- lang: eng
text: The plant hormone auxin and its directional transport are known to play a
crucial role in defining the embryonic axis and subsequent development of the
body plan. Although the role of PIN auxin efflux transporters has been clearly
assigned during embryonic shoot and root specification, the role of the auxin
influx carriers AUX1 and LIKE-AUX1 (LAX) proteins is not well established. Here,
we used chemical and genetic tools on Brassica napus microspore-derived embryos
and Arabidopsis thaliana zygotic embryos, and demonstrate that AUX1, LAX1 and
LAX2 are required for both shoot and root pole formation, in concert with PIN
efflux carriers. Furthermore, we uncovered a positive-feedback loop betweenMONOPTEROS(ARF5)-dependent
auxin signalling and auxin transport. ThisMONOPTEROSdependent transcriptional
regulation of auxin influx (AUX1, LAX1 and LAX2) and auxin efflux (PIN1 and PIN4)
carriers by MONOPTEROS helps to maintain proper auxin transport to the root tip.
These results indicate that auxin-dependent cell specification during embryo development
requires balanced auxin transport involving both influx and efflux mechanisms,
and that this transport is maintained by a positive transcriptional feedback on
auxin signalling.
acknowledgement: W.G. is a post-doctoral fellow of the Research Foundation Flanders.
H.S.R. is supported by Employment of Best Young Scientists for International Cooperation
Empowerment [CZ.1.07/2.3.00/30.0037], co-financed by the European Social Fund and
the state budget of the Czech Republic. Mi.S. was funded by the Ramón y Cajal program.
This work was supported by the European Research Council [project ERC-2011-StG-20101109-PSDP],
project ‘CEITEC – Central European Institute of Technology’ [CZ.1.05/1.1.00/02.0068],
the European Social Fund [CZ.1.07/2.3.00/20.0043] and the Czech Science Foundation
GACR [GA13-40637S] to J.F. We acknowledge funding from the Biological and Biotechnological
Science Research Council (BBSRC) and Engineering Physics Science Research Council
(EPSRC) to R.S. and M.B
author:
- first_name: Hélène
full_name: Robert, Hélène
last_name: Robert
- first_name: Wim
full_name: Grunewald, Wim
last_name: Grunewald
- first_name: Michael
full_name: Sauer, Michael
last_name: Sauer
- first_name: Bernard
full_name: Cannoot, Bernard
last_name: Cannoot
- first_name: Mercedes
full_name: Soriano, Mercedes
last_name: Soriano
- first_name: Ranjan
full_name: Swarup, Ranjan
last_name: Swarup
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
- first_name: Malcolm
full_name: Bennett, Malcolm
last_name: Bennett
- first_name: Kim
full_name: Boutilier, Kim
last_name: Boutilier
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Robert H, Grunewald W, Sauer M, et al. Plant embryogenesis requires AUX/LAX-mediated
auxin influx. Development. 2015;142(4):702-711. doi:10.1242/dev.115832
apa: Robert, H., Grunewald, W., Sauer, M., Cannoot, B., Soriano, M., Swarup, R.,
… Friml, J. (2015). Plant embryogenesis requires AUX/LAX-mediated auxin influx.
Development. Company of Biologists. https://doi.org/10.1242/dev.115832
chicago: Robert, Hélène, Wim Grunewald, Michael Sauer, Bernard Cannoot, Mercedes
Soriano, Ranjan Swarup, Dolf Weijers, Malcolm Bennett, Kim Boutilier, and Jiří
Friml. “Plant Embryogenesis Requires AUX/LAX-Mediated Auxin Influx.” Development.
Company of Biologists, 2015. https://doi.org/10.1242/dev.115832.
ieee: H. Robert et al., “Plant embryogenesis requires AUX/LAX-mediated auxin
influx,” Development, vol. 142, no. 4. Company of Biologists, pp. 702–711,
2015.
ista: Robert H, Grunewald W, Sauer M, Cannoot B, Soriano M, Swarup R, Weijers D,
Bennett M, Boutilier K, Friml J. 2015. Plant embryogenesis requires AUX/LAX-mediated
auxin influx. Development. 142(4), 702–711.
mla: Robert, Hélène, et al. “Plant Embryogenesis Requires AUX/LAX-Mediated Auxin
Influx.” Development, vol. 142, no. 4, Company of Biologists, 2015, pp.
702–11, doi:10.1242/dev.115832.
short: H. Robert, W. Grunewald, M. Sauer, B. Cannoot, M. Soriano, R. Swarup, D.
Weijers, M. Bennett, K. Boutilier, J. Friml, Development 142 (2015) 702–711.
date_created: 2018-12-11T11:54:26Z
date_published: 2015-02-15T00:00:00Z
date_updated: 2021-01-12T06:53:43Z
day: '15'
department:
- _id: JiFr
doi: 10.1242/dev.115832
ec_funded: 1
intvolume: ' 142'
issue: '4'
language:
- iso: eng
month: '02'
oa_version: None
page: 702 - 711
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Development
publication_status: published
publisher: Company of Biologists
publist_id: '5231'
quality_controlled: '1'
scopus_import: 1
status: public
title: Plant embryogenesis requires AUX/LAX-mediated auxin influx
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 142
year: '2015'
...
---
_id: '1871'
abstract:
- lang: eng
text: The plant hormone auxin is a key regulator of plant growth and development.
Differences in auxin distribution within tissues are mediated by the polar auxin
transport machinery, and cellular auxin responses occur depending on changes in
cellular auxin levels. Multiple receptor systems at the cell surface and in the
interior operate to sense and interpret fluctuations in auxin distribution that
occur during plant development. Until now, three proteins or protein complexes
that can bind auxin have been identified. SCFTIR1 [a SKP1-cullin-1-F-box complex
that contains transport inhibitor response 1 (TIR1) as the F-box protein] and
S-phase-kinaseassociated protein 2 (SKP2) localize to the nucleus, whereas auxinbinding
protein 1 (ABP1), predominantly associates with the endoplasmic reticulum and
cell surface. In this Cell Science at a Glance article, we summarize recent discoveries
in the field of auxin transport and signaling that have led to the identification
of new components of these pathways, as well as their mutual interaction.
acknowledgement: This work was supported by the European Research Council [project
ERC-2011-StG-20101109-PSDP]; European Social Fund [grant number CZ.1.07/2.3.00/20.0043]
and the Czech Science Foundation GAČR [grant number GA13-40637S]
author:
- first_name: Peter
full_name: Grones, Peter
id: 399876EC-F248-11E8-B48F-1D18A9856A87
last_name: Grones
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Grones P, Friml J. Auxin transporters and binding proteins at a glance. Journal
of Cell Science. 2015;128(1):1-7. doi:10.1242/jcs.159418
apa: Grones, P., & Friml, J. (2015). Auxin transporters and binding proteins
at a glance. Journal of Cell Science. Company of Biologists. https://doi.org/10.1242/jcs.159418
chicago: Grones, Peter, and Jiří Friml. “Auxin Transporters and Binding Proteins
at a Glance.” Journal of Cell Science. Company of Biologists, 2015. https://doi.org/10.1242/jcs.159418.
ieee: P. Grones and J. Friml, “Auxin transporters and binding proteins at a glance,”
Journal of Cell Science, vol. 128, no. 1. Company of Biologists, pp. 1–7,
2015.
ista: Grones P, Friml J. 2015. Auxin transporters and binding proteins at a glance.
Journal of Cell Science. 128(1), 1–7.
mla: Grones, Peter, and Jiří Friml. “Auxin Transporters and Binding Proteins at
a Glance.” Journal of Cell Science, vol. 128, no. 1, Company of Biologists,
2015, pp. 1–7, doi:10.1242/jcs.159418.
short: P. Grones, J. Friml, Journal of Cell Science 128 (2015) 1–7.
date_created: 2018-12-11T11:54:28Z
date_published: 2015-01-01T00:00:00Z
date_updated: 2021-01-12T06:53:45Z
day: '01'
ddc:
- '570'
department:
- _id: JiFr
doi: 10.1242/jcs.159418
file:
- access_level: open_access
checksum: 24c779f4cd9d549ca6833e26f486be27
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:11:00Z
date_updated: 2020-07-14T12:45:19Z
file_id: '4852'
file_name: IST-2016-563-v1+1_1.full.pdf
file_size: 1688844
relation: main_file
file_date_updated: 2020-07-14T12:45:19Z
has_accepted_license: '1'
intvolume: ' 128'
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Submitted Version
page: 1 - 7
publication: Journal of Cell Science
publication_status: published
publisher: Company of Biologists
publist_id: '5225'
pubrep_id: '563'
quality_controlled: '1'
scopus_import: 1
status: public
title: Auxin transporters and binding proteins at a glance
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 128
year: '2015'
...
---
_id: '1879'
abstract:
- lang: eng
text: When electron microscopy (EM) was introduced in the 1930s it gave scientists
their first look into the nanoworld of cells. Over the last 80 years EM has vastly
increased our understanding of the complex cellular structures that underlie the
diverse functions that cells need to maintain life. One drawback that has been
difficult to overcome was the inherent lack of volume information, mainly due
to the limit on the thickness of sections that could be viewed in a transmission
electron microscope (TEM). For many years scientists struggled to achieve three-dimensional
(3D) EM using serial section reconstructions, TEM tomography, and scanning EM
(SEM) techniques such as freeze-fracture. Although each technique yielded some
special information, they required a significant amount of time and specialist
expertise to obtain even a very small 3D EM dataset. Almost 20 years ago scientists
began to exploit SEMs to image blocks of embedded tissues and perform serial sectioning
of these tissues inside the SEM chamber. Using first focused ion beams (FIB) and
subsequently robotic ultramicrotomes (serial block-face, SBF-SEM) microscopists
were able to collect large volumes of 3D EM information at resolutions that could
address many important biological questions, and do so in an efficient manner.
We present here some examples of 3D EM taken from the many diverse specimens that
have been imaged in our core facility. We propose that the next major step forward
will be to efficiently correlate functional information obtained using light microscopy
(LM) with 3D EM datasets to more completely investigate the important links between
cell structures and their functions.
acknowledgement: The Zeiss Merlin with Gatan 3View2XP and Zeiss Auriga were acquired
through a CLEM grant from Minister Ingrid Lieten to the VIB Bio-Imaging-Core. Michiel
Krols and Saskia Lippens are the recipients of a fellowship from the FWO (Fonds
Wetenschappelijk Onderzoek) of Flanders.
author:
- first_name: A
full_name: Kremer, A
last_name: Kremer
- first_name: Stefaan
full_name: Lippens, Stefaan
last_name: Lippens
- first_name: Sonia
full_name: Bartunkova, Sonia
last_name: Bartunkova
- first_name: Bob
full_name: Asselbergh, Bob
last_name: Asselbergh
- first_name: Cendric
full_name: Blanpain, Cendric
last_name: Blanpain
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: A
full_name: Goossens, A
last_name: Goossens
- first_name: Matthew
full_name: Holt, Matthew
last_name: Holt
- first_name: Sophie
full_name: Janssens, Sophie
last_name: Janssens
- first_name: Michiel
full_name: Krols, Michiel
last_name: Krols
- first_name: Jean
full_name: Larsimont, Jean
last_name: Larsimont
- first_name: Conor
full_name: Mc Guire, Conor
last_name: Mc Guire
- first_name: Moritz
full_name: Nowack, Moritz
last_name: Nowack
- first_name: Xavier
full_name: Saelens, Xavier
last_name: Saelens
- first_name: Andreas
full_name: Schertel, Andreas
last_name: Schertel
- first_name: B
full_name: Schepens, B
last_name: Schepens
- first_name: M
full_name: Slezak, M
last_name: Slezak
- first_name: Vincent
full_name: Timmerman, Vincent
last_name: Timmerman
- first_name: Clara
full_name: Theunis, Clara
last_name: Theunis
- first_name: Ronald
full_name: Van Brempt, Ronald
last_name: Van Brempt
- first_name: Y
full_name: Visser, Y
last_name: Visser
- first_name: Christophe
full_name: Guérin, Christophe
last_name: Guérin
citation:
ama: Kremer A, Lippens S, Bartunkova S, et al. Developing 3D SEM in a broad biological
context. Journal of Microscopy. 2015;259(2):80-96. doi:10.1111/jmi.12211
apa: Kremer, A., Lippens, S., Bartunkova, S., Asselbergh, B., Blanpain, C., Fendrych,
M., … Guérin, C. (2015). Developing 3D SEM in a broad biological context. Journal
of Microscopy. Wiley-Blackwell. https://doi.org/10.1111/jmi.12211
chicago: Kremer, A, Stefaan Lippens, Sonia Bartunkova, Bob Asselbergh, Cendric Blanpain,
Matyas Fendrych, A Goossens, et al. “Developing 3D SEM in a Broad Biological Context.”
Journal of Microscopy. Wiley-Blackwell, 2015. https://doi.org/10.1111/jmi.12211.
ieee: A. Kremer et al., “Developing 3D SEM in a broad biological context,”
Journal of Microscopy, vol. 259, no. 2. Wiley-Blackwell, pp. 80–96, 2015.
ista: Kremer A, Lippens S, Bartunkova S, Asselbergh B, Blanpain C, Fendrych M, Goossens
A, Holt M, Janssens S, Krols M, Larsimont J, Mc Guire C, Nowack M, Saelens X,
Schertel A, Schepens B, Slezak M, Timmerman V, Theunis C, Van Brempt R, Visser
Y, Guérin C. 2015. Developing 3D SEM in a broad biological context. Journal of
Microscopy. 259(2), 80–96.
mla: Kremer, A., et al. “Developing 3D SEM in a Broad Biological Context.” Journal
of Microscopy, vol. 259, no. 2, Wiley-Blackwell, 2015, pp. 80–96, doi:10.1111/jmi.12211.
short: A. Kremer, S. Lippens, S. Bartunkova, B. Asselbergh, C. Blanpain, M. Fendrych,
A. Goossens, M. Holt, S. Janssens, M. Krols, J. Larsimont, C. Mc Guire, M. Nowack,
X. Saelens, A. Schertel, B. Schepens, M. Slezak, V. Timmerman, C. Theunis, R.
Van Brempt, Y. Visser, C. Guérin, Journal of Microscopy 259 (2015) 80–96.
date_created: 2018-12-11T11:54:30Z
date_published: 2015-08-01T00:00:00Z
date_updated: 2021-01-12T06:53:48Z
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title: Developing 3D SEM in a broad biological context
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