---
_id: '6269'
abstract:
- lang: eng
text: 'Clathrin-Mediated Endocytosis (CME) is an aspect of cellular trafficking
that is constantly regulated for mediating developmental and physiological responses.
The main aim of my thesis is to decipher the basic mechanisms of CME and post-endocytic
trafficking in the whole multicellular organ systems of Arabidopsis. The first
chapter of my thesis describes the search for new components involved in CME.
Tandem affinity purification was conducted using CLC and its interacting partners
were identified. Amongst the identified proteins were the Auxilin-likes1 and 2
(Axl1/2), putative uncoating factors, for which we made a full functional analysis.
Over-expression of Axl1/2 causes extreme modifications in the dynamics of the
machinery proteins and inhibition of endocytosis altogether. However the loss
of function of the axl1/2 did not present any cellular or physiological phenotype,
meaning Auxilin-likes do not form the major uncoating machinery. The second chapter
of my thesis describes the establishment/utilisation of techniques to capture
the dynamicity and the complexity of CME and post-endocytic trafficking. We have
studied the development of endocytic pits at the PM – specifically, the mode of
membrane remodeling during pit development and the role of actin in it, given
plant cells possess high turgor pressure. Utilizing the improved z-resolution
of TIRF and VAEM techniques, we captured the time-lapse of the endocytic events
at the plasma membrane; and using particle detection software, we quantitatively
analysed all the endocytic trajectories in an unbiased way to obtain the endocytic
rate of the system. This together with the direct analysis of cargo internalisation
from the PM provided an estimate on the endocytic potential of the cell. We also
developed a methodology for ultrastructural analysis of different populations
of Clathrin-Coated Structures (CCSs) in both PM and endomembranes in unroofed
protoplasts. Structural analysis, together with the intensity profile of CCSs
at the PM show that the mode of CCP development at the PM follows ‘Constant curvature
model’; meaning that clathrin polymerisation energy is a major contributing factor
of membrane remodeling. In addition, other analyses clearly show that actin is
not required for membrane remodeling during invagination or any other step of
CCP development, despite the prevalent high turgor pressure. However, actin is
essential in orchestrating the post-endocytic trafficking of CCVs facilitating
the EE formation. We also observed that the uncoating process post-endocytosis
is not immediate; an alternative mechanism of uncoating – Sequential multi-step
process – functions in the cell. Finally we also looked at one of the important
physiological stimuli modulating the process – hormone, auxin. auxin has been
known to influence CME before. We have made a detailed study on the concentration-time
based effect of auxin on the machinery proteins, CCP development, and the specificity
of cargoes endocytosed. To this end, we saw no general effect of auxin on CME
at earlier time points. However, very low concentration of IAA, such as 50nM,
accelerates endocytosis of specifically PIN2 through CME. Such a tight regulatory
control with high specificity to PIN2 could be essential in modulating its polarity. '
acknowledged_ssus:
- _id: Bio
- _id: EM-Fac
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Madhumitha
full_name: Narasimhan, Madhumitha
id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
last_name: Narasimhan
orcid: 0000-0002-8600-0671
citation:
ama: Narasimhan M. Clathrin-Mediated endocytosis, post-endocytic trafficking and
their regulatory controls in plants . 2019. doi:10.15479/at:ista:th1075
apa: Narasimhan, M. (2019). Clathrin-Mediated endocytosis, post-endocytic trafficking
and their regulatory controls in plants . Institute of Science and Technology
Austria. https://doi.org/10.15479/at:ista:th1075
chicago: Narasimhan, Madhumitha. “Clathrin-Mediated Endocytosis, Post-Endocytic
Trafficking and Their Regulatory Controls in Plants .” Institute of Science and
Technology Austria, 2019. https://doi.org/10.15479/at:ista:th1075.
ieee: M. Narasimhan, “Clathrin-Mediated endocytosis, post-endocytic trafficking
and their regulatory controls in plants ,” Institute of Science and Technology
Austria, 2019.
ista: Narasimhan M. 2019. Clathrin-Mediated endocytosis, post-endocytic trafficking
and their regulatory controls in plants . Institute of Science and Technology
Austria.
mla: Narasimhan, Madhumitha. Clathrin-Mediated Endocytosis, Post-Endocytic Trafficking
and Their Regulatory Controls in Plants . Institute of Science and Technology
Austria, 2019, doi:10.15479/at:ista:th1075.
short: M. Narasimhan, Clathrin-Mediated Endocytosis, Post-Endocytic Trafficking
and Their Regulatory Controls in Plants , Institute of Science and Technology
Austria, 2019.
date_created: 2019-04-09T14:37:06Z
date_published: 2019-02-04T00:00:00Z
date_updated: 2023-09-08T11:43:03Z
day: '04'
ddc:
- '575'
degree_awarded: PhD
department:
- _id: JiFr
doi: 10.15479/at:ista:th1075
file:
- access_level: open_access
checksum: c958f27dd752712886e7e2638b847a3c
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:18Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6270'
file_name: Supplementary_movie_1.avi
file_size: 5402078
relation: main_file
- access_level: open_access
checksum: 8786fdc29c62987c0aad3c866a4d3691
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:18Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6271'
file_name: 3.7_supplementary_movie_10.avi
file_size: 5927736
relation: main_file
- access_level: open_access
checksum: 25f784c5159d6f4d966b2f9b371ebaf6
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:18Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6272'
file_name: 3.7_supplementary_movie_9.avi
file_size: 9570210
relation: main_file
- access_level: open_access
checksum: 917069272a7a08d1f38224d5e12765d6
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:18Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6273'
file_name: 3.7_supplementary_movie_8.avi
file_size: 2827360
relation: main_file
- access_level: open_access
checksum: 81e74f5ca0ad70050504f18192236dc0
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:18Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6274'
file_name: 3.7_supplementary_movie_7.avi
file_size: 5771410
relation: main_file
- access_level: open_access
checksum: 47eb37b27a2930252713924307ea8c6f
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:18Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6275'
file_name: 3.7_supplementary_movie_6.avi
file_size: 1113486
relation: main_file
- access_level: open_access
checksum: f68f66721041ce84e331959c9a5779c3
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:18Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6276'
file_name: 3.7_supplementary_movie_5.avi
file_size: 1057232
relation: main_file
- access_level: open_access
checksum: 67c01cefab51b363c5e214fe4cd671f3
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:23Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6277'
file_name: 3.7_supplementary_movie_3.avi
file_size: 127472916
relation: main_file
- access_level: open_access
checksum: e5a397edbee05b8821e2b19b3c1a9260
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:19Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6278'
file_name: 3.7_supplementary_movie_4.avi
file_size: 3181238
relation: main_file
- access_level: open_access
checksum: 32d92b2a9277f956fdb0b42351d07c0b
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:19Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6279'
file_name: 3.7_supplementary_movie_2.avi
file_size: 5970952
relation: main_file
- access_level: open_access
checksum: efe7001f5d9a8c61e631e12d5f324ade
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:21Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6280'
file_name: 3.7_Supplementary_movie_1.avi
file_size: 39835236
relation: main_file
- access_level: open_access
checksum: eeb0a5603c6449c5f34eacd5ff0b3a16
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:21Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6281'
file_name: 2.5_Suppl_Movie_4_AP2A1_TagRFP.avi
file_size: 3696740
relation: main_file
- access_level: open_access
checksum: 8e7c00ef6223bf0e177deb168338af13
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:21Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6282'
file_name: 2.5_Suppl_Movie_3_TPLATE_GFP.avi
file_size: 6741232
relation: main_file
- access_level: open_access
checksum: 3636006a7cb709a7543d6581e359b28d
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:22Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6283'
file_name: 2.5_Suppl_Movie_2_CLC_GFP.avi
file_size: 2445946
relation: main_file
- access_level: open_access
checksum: 39ca5519a6e9a38356e7b3704004fea7
content_type: video/x-msvideo
creator: dernst
date_created: 2019-04-09T14:35:22Z
date_updated: 2021-02-11T23:30:15Z
embargo: 2020-02-11
file_id: '6284'
file_name: 2.5_Suppl_Movie_1_CLC_GFPxAxl1_mcherry.avi
file_size: 58594
relation: main_file
- access_level: open_access
checksum: 4fcdaa3a6c645514a3b3205f0f69dc76
content_type: application/pdf
creator: dernst
date_created: 2019-04-09T14:35:33Z
date_updated: 2021-02-11T11:17:15Z
embargo: 2020-02-11
file_id: '6285'
file_name: 2019_Thesis_Narasimhan.pdf
file_size: 10553937
relation: main_file
- access_level: closed
checksum: 268f0b6bad21d5f0d671e5d4b88104a7
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: dernst
date_created: 2019-04-09T14:35:36Z
date_updated: 2020-07-14T12:47:26Z
embargo_to: open_access
file_id: '6286'
file_name: 2019_Thesis_Narasimhan_source.docx
file_size: 135291990
relation: source_file
file_date_updated: 2021-02-11T23:30:15Z
has_accepted_license: '1'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: '138'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '412'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
title: 'Clathrin-Mediated endocytosis, post-endocytic trafficking and their regulatory
controls in plants '
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2019'
...
---
_id: '6351'
abstract:
- lang: eng
text: "A process of restorative patterning in plant roots correctly replaces eliminated
cells to heal local injuries despite the absence of cell migration, which underpins
wound healing in animals. \r\n\r\nPatterning in plants relies on oriented cell
divisions and acquisition of specific cell identities. Plants regularly endure
wounds caused by abiotic or biotic environmental stimuli and have developed extraordinary
abilities to restore their tissues after injuries. Here, we provide insight into
a mechanism of restorative patterning that repairs tissues after wounding. Laser-assisted
elimination of different cells in Arabidopsis root combined with live-imaging
tracking during vertical growth allowed analysis of the regeneration processes
in vivo. Specifically, the cells adjacent to the inner side of the injury re-activated
their stem cell transcriptional programs. They accelerated their progression through
cell cycle, coordinately changed the cell division orientation, and ultimately
acquired de novo the correct cell fates to replace missing cells. These observations
highlight existence of unknown intercellular positional signaling and demonstrate
the capability of specified cells to re-acquire stem cell programs as a crucial
part of the plant-specific mechanism of wound healing."
acknowledged_ssus:
- _id: Bio
article_processing_charge: No
author:
- first_name: Petra
full_name: Marhavá, Petra
id: 44E59624-F248-11E8-B48F-1D18A9856A87
last_name: Marhavá
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
orcid: 0000-0001-8295-2926
- first_name: Saiko
full_name: Yoshida, Saiko
id: 2E46069C-F248-11E8-B48F-1D18A9856A87
last_name: Yoshida
- first_name: Peter
full_name: Marhavy, Peter
id: 3F45B078-F248-11E8-B48F-1D18A9856A87
last_name: Marhavy
orcid: 0000-0001-5227-5741
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Marhavá P, Hörmayer L, Yoshida S, Marhavý P, Benková E, Friml J. Re-activation
of stem cell pathways for pattern restoration in plant wound healing. Cell.
2019;177(4):957-969.e13. doi:10.1016/j.cell.2019.04.015
apa: Marhavá, P., Hörmayer, L., Yoshida, S., Marhavý, P., Benková, E., & Friml,
J. (2019). Re-activation of stem cell pathways for pattern restoration in plant
wound healing. Cell. Elsevier. https://doi.org/10.1016/j.cell.2019.04.015
chicago: Marhavá, Petra, Lukas Hörmayer, Saiko Yoshida, Peter Marhavý, Eva Benková,
and Jiří Friml. “Re-Activation of Stem Cell Pathways for Pattern Restoration in
Plant Wound Healing.” Cell. Elsevier, 2019. https://doi.org/10.1016/j.cell.2019.04.015.
ieee: P. Marhavá, L. Hörmayer, S. Yoshida, P. Marhavý, E. Benková, and J. Friml,
“Re-activation of stem cell pathways for pattern restoration in plant wound healing,”
Cell, vol. 177, no. 4. Elsevier, p. 957–969.e13, 2019.
ista: Marhavá P, Hörmayer L, Yoshida S, Marhavý P, Benková E, Friml J. 2019. Re-activation
of stem cell pathways for pattern restoration in plant wound healing. Cell. 177(4),
957–969.e13.
mla: Marhavá, Petra, et al. “Re-Activation of Stem Cell Pathways for Pattern Restoration
in Plant Wound Healing.” Cell, vol. 177, no. 4, Elsevier, 2019, p. 957–969.e13,
doi:10.1016/j.cell.2019.04.015.
short: P. Marhavá, L. Hörmayer, S. Yoshida, P. Marhavý, E. Benková, J. Friml, Cell
177 (2019) 957–969.e13.
date_created: 2019-04-28T21:59:14Z
date_published: 2019-05-02T00:00:00Z
date_updated: 2024-03-28T23:30:10Z
day: '02'
ddc:
- '570'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1016/j.cell.2019.04.015
ec_funded: 1
external_id:
isi:
- '000466843000015'
pmid:
- '31051107'
file:
- access_level: open_access
checksum: 4ceba04a96a74f5092ec3ce2c579a0c7
content_type: application/pdf
creator: dernst
date_created: 2019-05-13T06:12:45Z
date_updated: 2020-07-14T12:47:28Z
file_id: '6411'
file_name: 2019_Cell_Marhava.pdf
file_size: 10272032
relation: main_file
file_date_updated: 2020-07-14T12:47:28Z
has_accepted_license: '1'
intvolume: ' 177'
isi: 1
issue: '4'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
page: 957-969.e13
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Cell
publication_identifier:
eissn:
- '10974172'
issn:
- '00928674'
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/specialized-plant-cells-regain-stem-cell-features-to-heal-wounds/
record:
- id: '9992'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Re-activation of stem cell pathways for pattern restoration in plant wound
healing
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 177
year: '2019'
...
---
_id: '6943'
abstract:
- lang: eng
text: Plants as sessile organisms are constantly under attack by herbivores, rough
environmental situations, or mechanical pressure. These challenges often lead
to the induction of wounds or destruction of already specified and developed tissues.
Additionally, wounding makes plants vulnerable to invasion by pathogens, which
is why wound signalling often triggers specific defence responses. To stay competitive
or, eventually, survive under these circumstances, plants need to regenerate efficiently,
which in rigid, tissue migration-incompatible plant tissues requires post-embryonic
patterning and organogenesis. Now, several studies used laser-assisted single
cell ablation in the Arabidopsis root tip as a minimal wounding proxy. Here, we
discuss their findings and put them into context of a broader spectrum of wound
signalling, pathogen responses and tissue as well as organ regeneration.
article_processing_charge: No
article_type: original
author:
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
orcid: 0000-0001-8295-2926
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Hörmayer L, Friml J. Targeted cell ablation-based insights into wound healing
and restorative patterning. Current Opinion in Plant Biology. 2019;52:124-130.
doi:10.1016/j.pbi.2019.08.006
apa: Hörmayer, L., & Friml, J. (2019). Targeted cell ablation-based insights
into wound healing and restorative patterning. Current Opinion in Plant Biology.
Elsevier. https://doi.org/10.1016/j.pbi.2019.08.006
chicago: Hörmayer, Lukas, and Jiří Friml. “Targeted Cell Ablation-Based Insights
into Wound Healing and Restorative Patterning.” Current Opinion in Plant Biology.
Elsevier, 2019. https://doi.org/10.1016/j.pbi.2019.08.006.
ieee: L. Hörmayer and J. Friml, “Targeted cell ablation-based insights into wound
healing and restorative patterning,” Current Opinion in Plant Biology,
vol. 52. Elsevier, pp. 124–130, 2019.
ista: Hörmayer L, Friml J. 2019. Targeted cell ablation-based insights into wound
healing and restorative patterning. Current Opinion in Plant Biology. 52, 124–130.
mla: Hörmayer, Lukas, and Jiří Friml. “Targeted Cell Ablation-Based Insights into
Wound Healing and Restorative Patterning.” Current Opinion in Plant Biology,
vol. 52, Elsevier, 2019, pp. 124–30, doi:10.1016/j.pbi.2019.08.006.
short: L. Hörmayer, J. Friml, Current Opinion in Plant Biology 52 (2019) 124–130.
date_created: 2019-10-14T07:00:24Z
date_published: 2019-12-01T00:00:00Z
date_updated: 2024-03-28T23:30:10Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.pbi.2019.08.006
ec_funded: 1
external_id:
isi:
- '000502890600017'
pmid:
- '31585333'
file:
- access_level: open_access
checksum: d6fd68a6e965f1efe3f0bf2d2070a616
content_type: application/pdf
creator: dernst
date_created: 2019-10-14T14:48:21Z
date_updated: 2020-07-14T12:47:45Z
file_id: '6946'
file_name: 2019_CurrentOpinionPlant_Hoermayer.pdf
file_size: 1659288
relation: main_file
file_date_updated: 2020-07-14T12:47:45Z
has_accepted_license: '1'
intvolume: ' 52'
isi: 1
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
page: 124-130
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Current Opinion in Plant Biology
publication_identifier:
issn:
- 1369-5266
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
record:
- id: '9992'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Targeted cell ablation-based insights into wound healing and restorative patterning
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 52
year: '2019'
...
---
_id: '6260'
abstract:
- lang: eng
text: Polar auxin transport plays a pivotal role in plant growth and development.
PIN auxin efflux carriers regulate directional auxin movement by establishing
local auxin maxima, minima, and gradients that drive multiple developmental processes
and responses to environmental signals. Auxin has been proposed to modulate its
own transport by regulating subcellular PIN trafficking via processes such as
clathrin-mediated PIN endocytosis and constitutive recycling. Here, we further
investigated the mechanisms by which auxin affects PIN trafficking by screening
auxin analogs and identified pinstatic acid (PISA) as a positive modulator of
polar auxin transport in Arabidopsis thaliana. PISA had an auxin-like effect on
hypocotyl elongation and adventitious root formation via positive regulation of
auxin transport. PISA did not activate SCFTIR1/AFB signaling and yet induced PIN
accumulation at the cell surface by inhibiting PIN internalization from the plasma
membrane. This work demonstrates PISA to be a promising chemical tool to dissect
the regulatory mechanisms behind subcellular PIN trafficking and auxin transport.
acknowledgement: "We thank Dr. H. Fukaki (University of Kobe), Dr. R. Offringa (Leiden
University), Dr. Jianwei Pan (Zhejiang Normal University), and Dr. M. Estelle (University
of California at San Diego) for providing mutants and transgenic line seeds.\r\nThis
work was supported by the Ministry of Education, Culture, Sports, Science, and Technology
(Grant-in-Aid for Scientific Research no. JP25114518 to K.H.), the Biotechnology
and Biological Sciences Research Council (award no. BB/L009366/1 to R.N. and S.K.),
and the European Union’s Horizon2020 program (European Research Council grant agreement
no. 742985 to J.F.)."
article_processing_charge: No
article_type: original
author:
- first_name: A
full_name: Oochi, A
last_name: Oochi
- first_name: Jakub
full_name: Hajny, Jakub
id: 4800CC20-F248-11E8-B48F-1D18A9856A87
last_name: Hajny
orcid: 0000-0003-2140-7195
- first_name: K
full_name: Fukui, K
last_name: Fukui
- first_name: Y
full_name: Nakao, Y
last_name: Nakao
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: M
full_name: Quareshy, M
last_name: Quareshy
- first_name: K
full_name: Takahashi, K
last_name: Takahashi
- first_name: T
full_name: Kinoshita, T
last_name: Kinoshita
- first_name: SR
full_name: Harborough, SR
last_name: Harborough
- first_name: S
full_name: Kepinski, S
last_name: Kepinski
- first_name: H
full_name: Kasahara, H
last_name: Kasahara
- first_name: RM
full_name: Napier, RM
last_name: Napier
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: KI
full_name: Hayashi, KI
last_name: Hayashi
citation:
ama: Oochi A, Hajny J, Fukui K, et al. Pinstatic acid promotes auxin transport by
inhibiting PIN internalization. Plant Physiology. 2019;180(2):1152-1165.
doi:10.1104/pp.19.00201
apa: Oochi, A., Hajny, J., Fukui, K., Nakao, Y., Gallei, M. C., Quareshy, M., …
Hayashi, K. (2019). Pinstatic acid promotes auxin transport by inhibiting PIN
internalization. Plant Physiology. ASPB. https://doi.org/10.1104/pp.19.00201
chicago: Oochi, A, Jakub Hajny, K Fukui, Y Nakao, Michelle C Gallei, M Quareshy,
K Takahashi, et al. “Pinstatic Acid Promotes Auxin Transport by Inhibiting PIN
Internalization.” Plant Physiology. ASPB, 2019. https://doi.org/10.1104/pp.19.00201.
ieee: A. Oochi et al., “Pinstatic acid promotes auxin transport by inhibiting
PIN internalization,” Plant Physiology, vol. 180, no. 2. ASPB, pp. 1152–1165,
2019.
ista: Oochi A, Hajny J, Fukui K, Nakao Y, Gallei MC, Quareshy M, Takahashi K, Kinoshita
T, Harborough S, Kepinski S, Kasahara H, Napier R, Friml J, Hayashi K. 2019. Pinstatic
acid promotes auxin transport by inhibiting PIN internalization. Plant Physiology.
180(2), 1152–1165.
mla: Oochi, A., et al. “Pinstatic Acid Promotes Auxin Transport by Inhibiting PIN
Internalization.” Plant Physiology, vol. 180, no. 2, ASPB, 2019, pp. 1152–65,
doi:10.1104/pp.19.00201.
short: A. Oochi, J. Hajny, K. Fukui, Y. Nakao, M.C. Gallei, M. Quareshy, K. Takahashi,
T. Kinoshita, S. Harborough, S. Kepinski, H. Kasahara, R. Napier, J. Friml, K.
Hayashi, Plant Physiology 180 (2019) 1152–1165.
date_created: 2019-04-09T08:38:20Z
date_published: 2019-06-01T00:00:00Z
date_updated: 2024-03-28T23:30:38Z
day: '01'
department:
- _id: JiFr
doi: 10.1104/pp.19.00201
ec_funded: 1
external_id:
isi:
- '000470086100045'
pmid:
- '30936248'
intvolume: ' 180'
isi: 1
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1104/pp.19.00201
month: '06'
oa: 1
oa_version: Published Version
page: 1152-1165
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Plant Physiology
publication_identifier:
eissn:
- 1532-2548
issn:
- 0032-0889
publication_status: published
publisher: ASPB
quality_controlled: '1'
related_material:
record:
- id: '11626'
relation: dissertation_contains
status: public
- id: '8822'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Pinstatic acid promotes auxin transport by inhibiting PIN internalization
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 180
year: '2019'
...
---
_id: '6627'
abstract:
- lang: eng
text: Cortical microtubule arrays in elongating epidermal cells in both the root
and stem of plants have the propensity of dynamic reorientations that are correlated
with the activation or inhibition of growth. Factors regulating plant growth,
among them the hormone auxin, have been recognized as regulators of microtubule
array orientations. Some previous work in the field has aimed at elucidating the
causal relationship between cell growth, the signaling of auxin or other growth-regulating
factors, and microtubule array reorientations, with various conclusions. Here,
we revisit this problem of causality with a comprehensive set of experiments in
Arabidopsis thaliana, using the now available pharmacological and genetic tools.
We use isolated, auxin-depleted hypocotyls, an experimental system allowing for
full control of both growth and auxin signaling. We demonstrate that reorientation
of microtubules is not directly triggered by an auxin signal during growth activation.
Instead, reorientation is triggered by the activation of the growth process itself
and is auxin-independent in its nature. We discuss these findings in the context
of previous relevant work, including that on the mechanical regulation of microtubule
array orientation.
article_number: '3337'
article_processing_charge: Yes
article_type: original
author:
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Adamowski M, Li L, Friml J. Reorientation of cortical microtubule arrays in
the hypocotyl of arabidopsis thaliana is induced by the cell growth process and
independent of auxin signaling. International Journal of Molecular Sciences.
2019;20(13). doi:10.3390/ijms20133337
apa: Adamowski, M., Li, L., & Friml, J. (2019). Reorientation of cortical microtubule
arrays in the hypocotyl of arabidopsis thaliana is induced by the cell growth
process and independent of auxin signaling. International Journal of Molecular
Sciences. MDPI. https://doi.org/10.3390/ijms20133337
chicago: Adamowski, Maciek, Lanxin Li, and Jiří Friml. “Reorientation of Cortical
Microtubule Arrays in the Hypocotyl of Arabidopsis Thaliana Is Induced by the
Cell Growth Process and Independent of Auxin Signaling.” International Journal
of Molecular Sciences. MDPI, 2019. https://doi.org/10.3390/ijms20133337.
ieee: M. Adamowski, L. Li, and J. Friml, “Reorientation of cortical microtubule
arrays in the hypocotyl of arabidopsis thaliana is induced by the cell growth
process and independent of auxin signaling,” International Journal of Molecular
Sciences, vol. 20, no. 13. MDPI, 2019.
ista: Adamowski M, Li L, Friml J. 2019. Reorientation of cortical microtubule arrays
in the hypocotyl of arabidopsis thaliana is induced by the cell growth process
and independent of auxin signaling. International Journal of Molecular Sciences.
20(13), 3337.
mla: Adamowski, Maciek, et al. “Reorientation of Cortical Microtubule Arrays in
the Hypocotyl of Arabidopsis Thaliana Is Induced by the Cell Growth Process and
Independent of Auxin Signaling.” International Journal of Molecular Sciences,
vol. 20, no. 13, 3337, MDPI, 2019, doi:10.3390/ijms20133337.
short: M. Adamowski, L. Li, J. Friml, International Journal of Molecular Sciences
20 (2019).
date_created: 2019-07-11T12:00:32Z
date_published: 2019-07-07T00:00:00Z
date_updated: 2024-03-28T23:30:44Z
day: '07'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.3390/ijms20133337
ec_funded: 1
external_id:
isi:
- '000477041100221'
pmid:
- '31284661'
file:
- access_level: open_access
checksum: dd9d1cbb933a72ceb666c9667890ac51
content_type: application/pdf
creator: dernst
date_created: 2019-07-17T06:17:15Z
date_updated: 2020-07-14T12:47:34Z
file_id: '6645'
file_name: 2019_JournalMolecularScience_Adamowski.pdf
file_size: 3330291
relation: main_file
file_date_updated: 2020-07-14T12:47:34Z
has_accepted_license: '1'
intvolume: ' 20'
isi: 1
issue: '13'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
- _id: B67AFEDC-15C9-11EA-A837-991A96BB2854
name: IST Austria Open Access Fund
publication: International Journal of Molecular Sciences
publication_identifier:
eissn:
- 1422-0067
publication_status: published
publisher: MDPI
quality_controlled: '1'
related_material:
record:
- id: '10083'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Reorientation of cortical microtubule arrays in the hypocotyl of arabidopsis
thaliana is induced by the cell growth process and independent of auxin signaling
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 20
year: '2019'
...
---
_id: '408'
abstract:
- lang: eng
text: Adventitious roots (AR) are de novo formed roots that emerge from any part
of the plant or from callus in tissue culture, except root tissue. The plant tissue
origin and the method by which they are induced determine the physiological properties
of emerged ARs. Hence, a standard method encompassing all types of AR does not
exist. Here we describe a method for the induction and analysis of AR that emerge
from the etiolated hypocotyl of dicot plants. The hypocotyl is formed during embryogenesis
and shows a determined developmental pattern which usually does not involve AR
formation. However, the hypocotyl shows propensity to form de novo roots under
specific circumstances such as removal of the root system, high humidity or flooding,
or during de-etiolation. The hypocotyl AR emerge from a pericycle-like cell layer
surrounding the vascular tissue of the central cylinder, which is reminiscent
to the developmental program of lateral roots. Here we propose an easy protocol
for in vitro hypocotyl AR induction from etiolated Arabidopsis seedlings.
alternative_title:
- MIMB
article_processing_charge: No
author:
- first_name: Hoang
full_name: Trinh, Hoang
last_name: Trinh
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Danny
full_name: Geelen, Danny
last_name: Geelen
citation:
ama: 'Trinh H, Verstraeten I, Geelen D. In vitro assay for induction of adventitious
rooting on intact arabidopsis hypocotyls. In: Root Development . Vol 1761.
Springer Nature; 2018:95-102. doi:10.1007/978-1-4939-7747-5_7'
apa: Trinh, H., Verstraeten, I., & Geelen, D. (2018). In vitro assay for induction
of adventitious rooting on intact arabidopsis hypocotyls. In Root Development
(Vol. 1761, pp. 95–102). Springer Nature. https://doi.org/10.1007/978-1-4939-7747-5_7
chicago: Trinh, Hoang, Inge Verstraeten, and Danny Geelen. “In Vitro Assay for Induction
of Adventitious Rooting on Intact Arabidopsis Hypocotyls.” In Root Development
, 1761:95–102. Springer Nature, 2018. https://doi.org/10.1007/978-1-4939-7747-5_7.
ieee: H. Trinh, I. Verstraeten, and D. Geelen, “In vitro assay for induction of
adventitious rooting on intact arabidopsis hypocotyls,” in Root Development
, vol. 1761, Springer Nature, 2018, pp. 95–102.
ista: 'Trinh H, Verstraeten I, Geelen D. 2018.In vitro assay for induction of adventitious
rooting on intact arabidopsis hypocotyls. In: Root Development . MIMB, vol. 1761,
95–102.'
mla: Trinh, Hoang, et al. “In Vitro Assay for Induction of Adventitious Rooting
on Intact Arabidopsis Hypocotyls.” Root Development , vol. 1761, Springer
Nature, 2018, pp. 95–102, doi:10.1007/978-1-4939-7747-5_7.
short: H. Trinh, I. Verstraeten, D. Geelen, in:, Root Development , Springer Nature,
2018, pp. 95–102.
date_created: 2018-12-11T11:46:18Z
date_published: 2018-03-01T00:00:00Z
date_updated: 2021-01-12T07:54:21Z
day: '01'
department:
- _id: JiFr
doi: 10.1007/978-1-4939-7747-5_7
external_id:
pmid:
- '29525951'
intvolume: ' 1761'
language:
- iso: eng
month: '03'
oa_version: None
page: 95 - 102
pmid: 1
publication: 'Root Development '
publication_identifier:
issn:
- 1064-3745
publication_status: published
publisher: Springer Nature
publist_id: '7421'
quality_controlled: '1'
scopus_import: '1'
status: public
title: In vitro assay for induction of adventitious rooting on intact arabidopsis
hypocotyls
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 1761
year: '2018'
...
---
_id: '411'
abstract:
- lang: eng
text: Immunolocalization is a valuable tool for cell biology research that allows
to rapidly determine the localization and expression levels of endogenous proteins.
In plants, whole-mount in situ immunolocalization remains a challenging method,
especially in tissues protected by waxy layers and complex cell wall carbohydrates.
Here, we present a robust method for whole-mount in situ immunolocalization in
primary root meristems and lateral root primordia in Arabidopsis thaliana. For
good epitope preservation, fixation is done in an alkaline paraformaldehyde/glutaraldehyde
mixture. This fixative is suitable for detecting a wide range of proteins, including
integral transmembrane proteins and proteins peripherally attached to the plasma
membrane. From initiation until emergence from the primary root, lateral root
primordia are surrounded by several layers of differentiated tissues with a complex
cell wall composition that interferes with the efficient penetration of all buffers.
Therefore, immunolocalization in early lateral root primordia requires a modified
method, including a strong solvent treatment for removal of hydrophobic barriers
and a specific cocktail of cell wall-degrading enzymes. The presented method allows
for easy, reliable, and high-quality in situ detection of the subcellular localization
of endogenous proteins in primary and lateral root meristems without the need
of time-consuming crosses or making translational fusions to fluorescent proteins.
alternative_title:
- Methods in Molecular Biology
author:
- first_name: Michael
full_name: Karampelias, Michael
last_name: Karampelias
- first_name: Ricardo
full_name: Tejos, Ricardo
last_name: Tejos
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
citation:
ama: 'Karampelias M, Tejos R, Friml J, Vanneste S. Optimized whole mount in situ
immunolocalization for Arabidopsis thaliana root meristems and lateral root primordia.
In: Ristova D, Barbez E, eds. Root Development. Methods and Protocols.
Vol 1761. MIMB. Springer; 2018:131-143. doi:10.1007/978-1-4939-7747-5_10'
apa: Karampelias, M., Tejos, R., Friml, J., & Vanneste, S. (2018). Optimized
whole mount in situ immunolocalization for Arabidopsis thaliana root meristems
and lateral root primordia. In D. Ristova & E. Barbez (Eds.), Root Development.
Methods and Protocols (Vol. 1761, pp. 131–143). Springer. https://doi.org/10.1007/978-1-4939-7747-5_10
chicago: Karampelias, Michael, Ricardo Tejos, Jiří Friml, and Steffen Vanneste.
“Optimized Whole Mount in Situ Immunolocalization for Arabidopsis Thaliana Root
Meristems and Lateral Root Primordia.” In Root Development. Methods and Protocols,
edited by Daniela Ristova and Elke Barbez, 1761:131–43. MIMB. Springer, 2018.
https://doi.org/10.1007/978-1-4939-7747-5_10.
ieee: M. Karampelias, R. Tejos, J. Friml, and S. Vanneste, “Optimized whole mount
in situ immunolocalization for Arabidopsis thaliana root meristems and lateral
root primordia,” in Root Development. Methods and Protocols, vol. 1761,
D. Ristova and E. Barbez, Eds. Springer, 2018, pp. 131–143.
ista: 'Karampelias M, Tejos R, Friml J, Vanneste S. 2018.Optimized whole mount in
situ immunolocalization for Arabidopsis thaliana root meristems and lateral root
primordia. In: Root Development. Methods and Protocols. Methods in Molecular Biology,
vol. 1761, 131–143.'
mla: Karampelias, Michael, et al. “Optimized Whole Mount in Situ Immunolocalization
for Arabidopsis Thaliana Root Meristems and Lateral Root Primordia.” Root
Development. Methods and Protocols, edited by Daniela Ristova and Elke Barbez,
vol. 1761, Springer, 2018, pp. 131–43, doi:10.1007/978-1-4939-7747-5_10.
short: M. Karampelias, R. Tejos, J. Friml, S. Vanneste, in:, D. Ristova, E. Barbez
(Eds.), Root Development. Methods and Protocols, Springer, 2018, pp. 131–143.
date_created: 2018-12-11T11:46:20Z
date_published: 2018-03-11T00:00:00Z
date_updated: 2021-01-12T07:54:34Z
day: '11'
department:
- _id: JiFr
doi: 10.1007/978-1-4939-7747-5_10
editor:
- first_name: Daniela
full_name: Ristova, Daniela
last_name: Ristova
- first_name: Elke
full_name: Barbez, Elke
last_name: Barbez
intvolume: ' 1761'
language:
- iso: eng
month: '03'
oa_version: None
page: 131 - 143
publication: Root Development. Methods and Protocols
publication_status: published
publisher: Springer
publist_id: '7418'
quality_controlled: '1'
scopus_import: 1
series_title: MIMB
status: public
title: Optimized whole mount in situ immunolocalization for Arabidopsis thaliana root
meristems and lateral root primordia
type: book_chapter
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 1761
year: '2018'
...
---
_id: '203'
abstract:
- lang: eng
text: Asymmetric auxin distribution is instrumental for the differential growth
that causes organ bending on tropic stimuli and curvatures during plant development.
Local differences in auxin concentrations are achieved mainly by polarized cellular
distribution of PIN auxin transporters, but whether other mechanisms involving
auxin homeostasis are also relevant for the formation of auxin gradients is not
clear. Here we show that auxin methylation is required for asymmetric auxin distribution
across the hypocotyl, particularly during its response to gravity. We found that
loss-of-function mutants in Arabidopsis IAA CARBOXYL METHYLTRANSFERASE1 (IAMT1)
prematurely unfold the apical hook, and that their hypocotyls are impaired in
gravitropic reorientation. This defect is linked to an auxin-dependent increase
in PIN gene expression, leading to an increased polar auxin transport and lack
of asymmetric distribution of PIN3 in the iamt1 mutant. Gravitropic reorientation
in the iamt1 mutant could be restored with either endodermis-specific expression
of IAMT1 or partial inhibition of polar auxin transport, which also results in
normal PIN gene expression levels. We propose that IAA methylation is necessary
in gravity-sensing cells to restrict polar auxin transport within the range of
auxin levels that allow for differential responses.
article_processing_charge: No
author:
- first_name: Mohamad
full_name: Abbas, Mohamad
id: 47E8FC1C-F248-11E8-B48F-1D18A9856A87
last_name: Abbas
- first_name: García J
full_name: Hernández, García J
last_name: Hernández
- first_name: Stephan
full_name: Pollmann, Stephan
last_name: Pollmann
- first_name: Sophia L
full_name: Samodelov, Sophia L
last_name: Samodelov
- first_name: Martina
full_name: Kolb, Martina
last_name: Kolb
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Ulrich Z
full_name: Hammes, Ulrich Z
last_name: Hammes
- first_name: Matias D
full_name: Zurbriggen, Matias D
last_name: Zurbriggen
- first_name: Miguel
full_name: Blázquez, Miguel
last_name: Blázquez
- first_name: David
full_name: Alabadí, David
last_name: Alabadí
citation:
ama: Abbas M, Hernández GJ, Pollmann S, et al. Auxin methylation is required for
differential growth in Arabidopsis. PNAS. 2018;115(26):6864-6869. doi:10.1073/pnas.1806565115
apa: Abbas, M., Hernández, G. J., Pollmann, S., Samodelov, S. L., Kolb, M., Friml,
J., … Alabadí, D. (2018). Auxin methylation is required for differential growth
in Arabidopsis. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1806565115
chicago: Abbas, Mohamad, García J Hernández, Stephan Pollmann, Sophia L Samodelov,
Martina Kolb, Jiří Friml, Ulrich Z Hammes, Matias D Zurbriggen, Miguel Blázquez,
and David Alabadí. “Auxin Methylation Is Required for Differential Growth in Arabidopsis.”
PNAS. National Academy of Sciences, 2018. https://doi.org/10.1073/pnas.1806565115.
ieee: M. Abbas et al., “Auxin methylation is required for differential growth
in Arabidopsis,” PNAS, vol. 115, no. 26. National Academy of Sciences,
pp. 6864–6869, 2018.
ista: Abbas M, Hernández GJ, Pollmann S, Samodelov SL, Kolb M, Friml J, Hammes UZ,
Zurbriggen MD, Blázquez M, Alabadí D. 2018. Auxin methylation is required for
differential growth in Arabidopsis. PNAS. 115(26), 6864–6869.
mla: Abbas, Mohamad, et al. “Auxin Methylation Is Required for Differential Growth
in Arabidopsis.” PNAS, vol. 115, no. 26, National Academy of Sciences,
2018, pp. 6864–69, doi:10.1073/pnas.1806565115.
short: M. Abbas, G.J. Hernández, S. Pollmann, S.L. Samodelov, M. Kolb, J. Friml,
U.Z. Hammes, M.D. Zurbriggen, M. Blázquez, D. Alabadí, PNAS 115 (2018) 6864–6869.
date_created: 2018-12-11T11:45:11Z
date_published: 2018-06-26T00:00:00Z
date_updated: 2023-09-08T13:24:40Z
day: '26'
department:
- _id: JiFr
doi: 10.1073/pnas.1806565115
ec_funded: 1
external_id:
isi:
- '000436245000096'
intvolume: ' 115'
isi: 1
issue: '26'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://eprints.nottingham.ac.uk/52388/
month: '06'
oa: 1
oa_version: None
page: 6864-6869
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '7710'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Auxin methylation is required for differential growth in Arabidopsis
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 115
year: '2018'
...
---
_id: '5830'
abstract:
- lang: eng
text: CLE peptides have been implicated in various developmental processes of plants
and mediate their responses to environmental stimuli. However, the biological
relevance of most CLE genes remains to be functionally characterized. Here, we
report that CLE9, which is expressed in stomata, acts as an essential regulator
in the induction of stomatal closure. Exogenous application of CLE9 peptides or
overexpression of CLE9 effectively led to stomatal closure and enhanced drought
tolerance, whereas CLE9 loss-of-function mutants were sensitivity to drought stress.
CLE9-induced stomatal closure was impaired in abscisic acid (ABA)-deficient mutants,
indicating that ABA is required for CLE9-medaited guard cell signalling. We further
deciphered that two guard cell ABA-signalling components, OST1 and SLAC1, were
responsible for CLE9-induced stomatal closure. MPK3 and MPK6 were activated by
the CLE9 peptide, and CLE9 peptides failed to close stomata in mpk3 and mpk6 mutants.
In addition, CLE9 peptides stimulated the induction of hydrogen peroxide (H2O2)
and nitric oxide (NO) synthesis associated with stomatal closure, which was abolished
in the NADPH oxidase-deficient mutants or nitric reductase mutants, respectively.
Collectively, our results reveal a novel ABA-dependent function of CLE9 in the
regulation of stomatal apertures, thereby suggesting a potential role of CLE9
in the stress acclimatization of plants.
article_processing_charge: No
author:
- first_name: Luosha
full_name: Zhang, Luosha
last_name: Zhang
- first_name: Xiong
full_name: Shi, Xiong
last_name: Shi
- first_name: Yutao
full_name: Zhang, Yutao
last_name: Zhang
- first_name: Jiajing
full_name: Wang, Jiajing
last_name: Wang
- first_name: Jingwei
full_name: Yang, Jingwei
last_name: Yang
- first_name: Takashi
full_name: Ishida, Takashi
last_name: Ishida
- first_name: Wenqian
full_name: Jiang, Wenqian
last_name: Jiang
- first_name: Xiangyu
full_name: Han, Xiangyu
last_name: Han
- first_name: Jingke
full_name: Kang, Jingke
last_name: Kang
- first_name: Xuening
full_name: Wang, Xuening
last_name: Wang
- first_name: Lixia
full_name: Pan, Lixia
last_name: Pan
- first_name: Shuo
full_name: Lv, Shuo
last_name: Lv
- first_name: Bing
full_name: Cao, Bing
last_name: Cao
- first_name: Yonghong
full_name: Zhang, Yonghong
last_name: Zhang
- first_name: Jinbin
full_name: Wu, Jinbin
last_name: Wu
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
- first_name: Zhubing
full_name: Hu, Zhubing
last_name: Hu
- first_name: Langjun
full_name: Cui, Langjun
last_name: Cui
- first_name: Shinichiro
full_name: Sawa, Shinichiro
last_name: Sawa
- first_name: Junmin
full_name: He, Junmin
last_name: He
- first_name: Guodong
full_name: Wang, Guodong
last_name: Wang
citation:
ama: Zhang L, Shi X, Zhang Y, et al. CLE9 peptide-induced stomatal closure is mediated
by abscisic acid, hydrogen peroxide, and nitric oxide in arabidopsis thaliana.
Plant Cell and Environment. 2018. doi:10.1111/pce.13475
apa: Zhang, L., Shi, X., Zhang, Y., Wang, J., Yang, J., Ishida, T., … Wang, G. (2018).
CLE9 peptide-induced stomatal closure is mediated by abscisic acid, hydrogen peroxide,
and nitric oxide in arabidopsis thaliana. Plant Cell and Environment. Wiley.
https://doi.org/10.1111/pce.13475
chicago: Zhang, Luosha, Xiong Shi, Yutao Zhang, Jiajing Wang, Jingwei Yang, Takashi
Ishida, Wenqian Jiang, et al. “CLE9 Peptide-Induced Stomatal Closure Is Mediated
by Abscisic Acid, Hydrogen Peroxide, and Nitric Oxide in Arabidopsis Thaliana.”
Plant Cell and Environment. Wiley, 2018. https://doi.org/10.1111/pce.13475.
ieee: L. Zhang et al., “CLE9 peptide-induced stomatal closure is mediated
by abscisic acid, hydrogen peroxide, and nitric oxide in arabidopsis thaliana,”
Plant Cell and Environment. Wiley, 2018.
ista: Zhang L, Shi X, Zhang Y, Wang J, Yang J, Ishida T, Jiang W, Han X, Kang J,
Wang X, Pan L, Lv S, Cao B, Zhang Y, Wu J, Han H, Hu Z, Cui L, Sawa S, He J, Wang
G. 2018. CLE9 peptide-induced stomatal closure is mediated by abscisic acid, hydrogen
peroxide, and nitric oxide in arabidopsis thaliana. Plant Cell and Environment.
mla: Zhang, Luosha, et al. “CLE9 Peptide-Induced Stomatal Closure Is Mediated by
Abscisic Acid, Hydrogen Peroxide, and Nitric Oxide in Arabidopsis Thaliana.” Plant
Cell and Environment, Wiley, 2018, doi:10.1111/pce.13475.
short: L. Zhang, X. Shi, Y. Zhang, J. Wang, J. Yang, T. Ishida, W. Jiang, X. Han,
J. Kang, X. Wang, L. Pan, S. Lv, B. Cao, Y. Zhang, J. Wu, H. Han, Z. Hu, L. Cui,
S. Sawa, J. He, G. Wang, Plant Cell and Environment (2018).
date_created: 2019-01-13T22:59:11Z
date_published: 2018-10-31T00:00:00Z
date_updated: 2023-09-11T12:43:31Z
day: '31'
department:
- _id: JiFr
doi: 10.1111/pce.13475
external_id:
isi:
- '000459014800021'
pmid:
- '30378140'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pubmed/30378140
month: '10'
oa: 1
oa_version: Published Version
pmid: 1
publication: Plant Cell and Environment
publication_identifier:
issn:
- '01407791'
publication_status: epub_ahead
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: CLE9 peptide-induced stomatal closure is mediated by abscisic acid, hydrogen
peroxide, and nitric oxide in arabidopsis thaliana
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2018'
...
---
_id: '428'
abstract:
- lang: eng
text: The plant hormone gibberellic acid (GA) is a crucial regulator of growth and
development. The main paradigm of GA signaling puts forward transcriptional regulation
via the degradation of DELLA transcriptional repressors. GA has also been shown
to regulate tropic responses by modulation of the plasma membrane incidence of
PIN auxin transporters by an unclear mechanism. Here we uncovered the cellular
and molecular mechanisms by which GA redirects protein trafficking and thus regulates
cell surface functionality. Photoconvertible reporters revealed that GA balances
the protein traffic between the vacuole degradation route and recycling back to
the cell surface. Low GA levels promote vacuolar delivery and degradation of multiple
cargos, including PIN proteins, whereas high GA levels promote their recycling
to the plasma membrane. This GA effect requires components of the retromer complex,
such as Sorting Nexin 1 (SNX1) and its interacting, microtubule (MT)-associated
protein, the Cytoplasmic Linker-Associated Protein (CLASP1). Accordingly, GA regulates
the subcellular distribution of SNX1 and CLASP1, and the intact MT cytoskeleton
is essential for the GA effect on trafficking. This GA cellular action occurs
through DELLA proteins that regulate the MT and retromer presumably via their
interaction partners Prefoldins (PFDs). Our study identified a branching of the
GA signaling pathway at the level of DELLA proteins, which, in parallel to regulating
transcription, also target by a nontranscriptional mechanism the retromer complex
acting at the intersection of the degradation and recycling trafficking routes.
By this mechanism, GA can redirect receptors and transporters to the cell surface,
thus coregulating multiple processes, including PIN-dependent auxin fluxes during
tropic responses.
acknowledgement: "We gratefully acknowledge M. Blázquez (Instituto de Biología Molecular
y Celular de Plantas), M. Fendrych, C. Cuesta Moliner (Institute of Science and
Technology Austria), M. Vanstraelen, M. Nowack (Center for Plant Systems Biology,
Ghent), C. Luschnig (Universitat fur Bodenkultur Wien, Vienna), S. Simon (Central
European Institute of Technology, Brno), C. Sommerville (Carnegie Institution for
Science), and Y. Gu (Penn State University) for making available the materials used
in this study;\r\n...funding from the European Research Council (ERC) under the
European Union’s Seventh Framework Programme (FP7/2007-2013)/ERC Grant Agreement
282300.\r\nCC BY NC ND"
article_processing_charge: No
author:
- first_name: Yuliya
full_name: Salanenka, Yuliya
id: 46DAAE7E-F248-11E8-B48F-1D18A9856A87
last_name: Salanenka
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Christian
full_name: Löfke, Christian
last_name: Löfke
- first_name: Kaori
full_name: Tabata, Kaori
id: 7DAAEDA4-02D0-11E9-B11A-A5A4D7DFFFD0
last_name: Tabata
- first_name: Satoshi
full_name: Naramoto, Satoshi
last_name: Naramoto
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Salanenka Y, Verstraeten I, Löfke C, et al. Gibberellin DELLA signaling targets
the retromer complex to redirect protein trafficking to the plasma membrane. PNAS.
2018;115(14):3716-3721. doi:10.1073/pnas.1721760115
apa: Salanenka, Y., Verstraeten, I., Löfke, C., Tabata, K., Naramoto, S., Glanc,
M., & Friml, J. (2018). Gibberellin DELLA signaling targets the retromer complex
to redirect protein trafficking to the plasma membrane. PNAS. National
Academy of Sciences. https://doi.org/10.1073/pnas.1721760115
chicago: Salanenka, Yuliya, Inge Verstraeten, Christian Löfke, Kaori Tabata, Satoshi
Naramoto, Matous Glanc, and Jiří Friml. “Gibberellin DELLA Signaling Targets the
Retromer Complex to Redirect Protein Trafficking to the Plasma Membrane.” PNAS.
National Academy of Sciences, 2018. https://doi.org/10.1073/pnas.1721760115.
ieee: Y. Salanenka et al., “Gibberellin DELLA signaling targets the retromer
complex to redirect protein trafficking to the plasma membrane,” PNAS,
vol. 115, no. 14. National Academy of Sciences, pp. 3716–3721, 2018.
ista: Salanenka Y, Verstraeten I, Löfke C, Tabata K, Naramoto S, Glanc M, Friml
J. 2018. Gibberellin DELLA signaling targets the retromer complex to redirect
protein trafficking to the plasma membrane. PNAS. 115(14), 3716–3721.
mla: Salanenka, Yuliya, et al. “Gibberellin DELLA Signaling Targets the Retromer
Complex to Redirect Protein Trafficking to the Plasma Membrane.” PNAS,
vol. 115, no. 14, National Academy of Sciences, 2018, pp. 3716–21, doi:10.1073/pnas.1721760115.
short: Y. Salanenka, I. Verstraeten, C. Löfke, K. Tabata, S. Naramoto, M. Glanc,
J. Friml, PNAS 115 (2018) 3716–3721.
date_created: 2018-12-11T11:46:25Z
date_published: 2018-04-03T00:00:00Z
date_updated: 2023-09-11T14:06:34Z
day: '03'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1073/pnas.1721760115
ec_funded: 1
external_id:
isi:
- '000429012500073'
file:
- access_level: open_access
checksum: 1fcf7223fb8f99559cfa80bd6f24ce44
content_type: application/pdf
creator: dernst
date_created: 2018-12-17T12:30:14Z
date_updated: 2020-07-14T12:46:26Z
file_id: '5700'
file_name: 2018_PNAS_Salanenka.pdf
file_size: 1924101
relation: main_file
file_date_updated: 2020-07-14T12:46:26Z
has_accepted_license: '1'
intvolume: ' 115'
isi: 1
issue: '14'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
page: ' 3716 - 3721'
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '7395'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Gibberellin DELLA signaling targets the retromer complex to redirect protein
trafficking to the plasma membrane
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 115
year: '2018'
...