[{"status":"public","type":"journal_article","_id":"2290","department":[{"_id":"JiFr"}],"date_updated":"2021-01-12T06:56:33Z","intvolume":" 110","month":"10","main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3791722/"}],"scopus_import":1,"oa_version":"Submitted Version","pmid":1,"abstract":[{"text":"The plant hormone indole-acetic acid (auxin) is essential for many aspects of plant development. Auxin-mediated growth regulation typically involves the establishment of an auxin concentration gradient mediated by polarly localized auxin transporters. The localization of auxin carriers and their amount at the plasma membrane are controlled by membrane trafficking processes such as secretion, endocytosis, and recycling. In contrast to endocytosis or recycling, how the secretory pathway mediates the localization of auxin carriers is not well understood. In this study we have used the differential cell elongation process during apical hook development to elucidate the mechanisms underlying the post-Golgi trafficking of auxin carriers in Arabidopsis. We show that differential cell elongation during apical hook development is defective in Arabidopsis mutant echidna (ech). ECH protein is required for the trans-Golgi network (TGN)-mediated trafficking of the auxin influx carrier AUX1 to the plasma membrane. In contrast, ech mutation only marginally perturbs the trafficking of the highly related auxin influx carrier LIKE-AUX1-3 or the auxin efflux carrier PIN-FORMED-3, both also involved in hook development. Electron tomography reveals that the trafficking defects in ech mutant are associated with the perturbation of secretory vesicle genesis from the TGN. Our results identify differential mechanisms for the post-Golgi trafficking of de novo-synthesized auxin carriers to plasma membrane from the TGN and reveal how trafficking of auxin influx carriers mediates the control of differential cell elongation in apical hook development.","lang":"eng"}],"volume":110,"issue":"40","language":[{"iso":"eng"}],"publication_status":"published","title":"ECHIDNA mediated post Golgi trafficking of auxin carriers for differential cell elongation","external_id":{"pmid":["24043780"]},"author":[{"first_name":"Yohann","full_name":"Boutté, Yohann","last_name":"Boutté"},{"first_name":"Kristoffer","full_name":"Jonsson, Kristoffer","last_name":"Jonsson"},{"last_name":"Mcfarlane","full_name":"Mcfarlane, Heather","first_name":"Heather"},{"first_name":"Errin","full_name":"Johnson, Errin","last_name":"Johnson"},{"first_name":"Delphine","last_name":"Gendre","full_name":"Gendre, Delphine"},{"last_name":"Swarup","full_name":"Swarup, Ranjan","first_name":"Ranjan"},{"last_name":"Friml","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Samuels","full_name":"Samuels, Lacey","first_name":"Lacey"},{"last_name":"Robert","full_name":"Robert, Stéphanie","first_name":"Stéphanie"},{"full_name":"Bhalerao, Rishikesh","last_name":"Bhalerao","first_name":"Rishikesh"}],"publist_id":"4639","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"ista":"Boutté Y, Jonsson K, Mcfarlane H, Johnson E, Gendre D, Swarup R, Friml J, Samuels L, Robert S, Bhalerao R. 2013. ECHIDNA mediated post Golgi trafficking of auxin carriers for differential cell elongation. PNAS. 110(40), 16259–16264.","chicago":"Boutté, Yohann, Kristoffer Jonsson, Heather Mcfarlane, Errin Johnson, Delphine Gendre, Ranjan Swarup, Jiří Friml, Lacey Samuels, Stéphanie Robert, and Rishikesh Bhalerao. “ECHIDNA Mediated Post Golgi Trafficking of Auxin Carriers for Differential Cell Elongation.” PNAS. National Academy of Sciences, 2013. https://doi.org/10.1073/pnas.1309057110.","ama":"Boutté Y, Jonsson K, Mcfarlane H, et al. ECHIDNA mediated post Golgi trafficking of auxin carriers for differential cell elongation. PNAS. 2013;110(40):16259-16264. doi:10.1073/pnas.1309057110","apa":"Boutté, Y., Jonsson, K., Mcfarlane, H., Johnson, E., Gendre, D., Swarup, R., … Bhalerao, R. (2013). ECHIDNA mediated post Golgi trafficking of auxin carriers for differential cell elongation. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1309057110","ieee":"Y. Boutté et al., “ECHIDNA mediated post Golgi trafficking of auxin carriers for differential cell elongation,” PNAS, vol. 110, no. 40. National Academy of Sciences, pp. 16259–16264, 2013.","short":"Y. Boutté, K. Jonsson, H. Mcfarlane, E. Johnson, D. Gendre, R. Swarup, J. Friml, L. Samuels, S. Robert, R. Bhalerao, PNAS 110 (2013) 16259–16264.","mla":"Boutté, Yohann, et al. “ECHIDNA Mediated Post Golgi Trafficking of Auxin Carriers for Differential Cell Elongation.” PNAS, vol. 110, no. 40, National Academy of Sciences, 2013, pp. 16259–64, doi:10.1073/pnas.1309057110."},"oa":1,"publisher":"National Academy of Sciences","quality_controlled":"1","date_created":"2018-12-11T11:56:48Z","doi":"10.1073/pnas.1309057110","date_published":"2013-10-01T00:00:00Z","page":"16259 - 16264","publication":"PNAS","day":"01","year":"2013"},{"scopus_import":"1","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1111/nph.12437"}],"month":"12","intvolume":" 200","abstract":[{"lang":"eng","text":"The mode of action of auxin is based on its non-uniform distribution within tissues and organs. Despite the wide use of several auxin analogues in research and agriculture, little is known about the specificity of different auxin-related transport and signalling processes towards these compounds. Using seedlings of Arabidopsis thaliana and suspension-cultured cells of Nicotiana tabacum (BY-2), the physiological activity of several auxin analogues was investigated, together with their capacity to induce auxin-dependent gene expression, to inhibit endocytosis and to be transported across the plasma membrane. This study shows that the specificity criteria for different auxin-related processes vary widely. Notably, the special behaviour of some synthetic auxin analogues suggests that they might be useful tools in investigations of the molecular mechanism of auxin action. Thus, due to their differential stimulatory effects on DR5 expression, indole-3-propionic (IPA) and 2,4,5-trichlorophenoxy acetic (2,4,5-T) acids can serve in studies of TRANSPORT INHIBITOR RESPONSE 1/AUXIN SIGNALLING F-BOX (TIR1/AFB)-mediated auxin signalling, and 5-fluoroindole-3-acetic acid (5-F-IAA) can help to discriminate between transcriptional and non-transcriptional pathways of auxin signalling. The results demonstrate that the major determinants for the auxin-like physiological potential of a particular compound are very complex and involve its chemical and metabolic stability, its ability to distribute in tissues in a polar manner and its activity towards auxin signalling machinery."}],"oa_version":"Published Version","volume":200,"issue":"4","ec_funded":1,"publication_status":"published","language":[{"iso":"eng"}],"type":"journal_article","article_type":"original","status":"public","_id":"2443","department":[{"_id":"JiFr"}],"date_updated":"2022-06-07T08:57:52Z","quality_controlled":"1","publisher":"Wiley","oa":1,"acknowledgement":"The authors thank Dr Christian Luschnig (University of Natural Resources and Life Sciences (BOKU), Vienna, Austria) for the anti-PIN2 antibody, Professor Mark Estelle (University of California, San Diego, CA, USA) for tir1-1 mutant seeds and, last but not least, to Dr David Morris for critical reading of the manuscript. We also thank Markéta Pařezová and Jana Stýblová for excellent technical assistance. This work was supported by the Grant Agency of the Czech Republic (P305/11/0797 to E.Z. and 13-40637S to J.F.), the Central European Institute of Technology project CZ.1.05/1.1.00/02.0068 from the European Regional Development Fund and by a European Research Council starting independent research grant ERC-2011-StG-20101109-PSDP (to J.F.).","page":"1034 - 1048","doi":"10.1111/nph.12437","date_published":"2013-12-01T00:00:00Z","date_created":"2018-12-11T11:57:41Z","year":"2013","day":"01","publication":"New Phytologist","project":[{"call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425","name":"Polarity and subcellular dynamics in plants","grant_number":"282300"}],"author":[{"orcid":"0000-0002-1998-6741","full_name":"Simon, Sibu","last_name":"Simon","first_name":"Sibu","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Kubeš","full_name":"Kubeš, Martin","first_name":"Martin"},{"full_name":"Baster, Pawel","last_name":"Baster","id":"3028BD74-F248-11E8-B48F-1D18A9856A87","first_name":"Pawel"},{"full_name":"Robert, Stéphanie","last_name":"Robert","first_name":"Stéphanie"},{"last_name":"Dobrev","full_name":"Dobrev, Petre","first_name":"Petre"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","last_name":"Friml"},{"first_name":"Jan","full_name":"Petrášek, Jan","last_name":"Petrášek"},{"first_name":"Eva","last_name":"Zažímalová","full_name":"Zažímalová, Eva"}],"publist_id":"4460","article_processing_charge":"No","title":"Defining the selectivity of processes along the auxin response chain: A study using auxin analogues","citation":{"ista":"Simon S, Kubeš M, Baster P, Robert S, Dobrev P, Friml J, Petrášek J, Zažímalová E. 2013. Defining the selectivity of processes along the auxin response chain: A study using auxin analogues. New Phytologist. 200(4), 1034–1048.","chicago":"Simon, Sibu, Martin Kubeš, Pawel Baster, Stéphanie Robert, Petre Dobrev, Jiří Friml, Jan Petrášek, and Eva Zažímalová. “Defining the Selectivity of Processes along the Auxin Response Chain: A Study Using Auxin Analogues.” New Phytologist. Wiley, 2013. https://doi.org/10.1111/nph.12437.","ieee":"S. Simon et al., “Defining the selectivity of processes along the auxin response chain: A study using auxin analogues,” New Phytologist, vol. 200, no. 4. Wiley, pp. 1034–1048, 2013.","short":"S. Simon, M. Kubeš, P. Baster, S. Robert, P. Dobrev, J. Friml, J. Petrášek, E. Zažímalová, New Phytologist 200 (2013) 1034–1048.","apa":"Simon, S., Kubeš, M., Baster, P., Robert, S., Dobrev, P., Friml, J., … Zažímalová, E. (2013). Defining the selectivity of processes along the auxin response chain: A study using auxin analogues. New Phytologist. Wiley. https://doi.org/10.1111/nph.12437","ama":"Simon S, Kubeš M, Baster P, et al. Defining the selectivity of processes along the auxin response chain: A study using auxin analogues. New Phytologist. 2013;200(4):1034-1048. doi:10.1111/nph.12437","mla":"Simon, Sibu, et al. “Defining the Selectivity of Processes along the Auxin Response Chain: A Study Using Auxin Analogues.” New Phytologist, vol. 200, no. 4, Wiley, 2013, pp. 1034–48, doi:10.1111/nph.12437."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"},{"_id":"2449","status":"public","type":"journal_article","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"ista":"Nodzyński T, Feraru M, Hirsch S, De Rycke R, Nicuales C, Van Leene J, De Jaeger G, Vanneste S, Friml J. 2013. Retromer subunits VPS35A and VPS29 mediate prevacuolar compartment (PVC) function in Arabidopsis. Molecular Plant. 6(6), 1849–1862.","chicago":"Nodzyński, Tomasz, Murguel Feraru, Sibylle Hirsch, Riet De Rycke, Claudiu Nicuales, Jelle Van Leene, Geert De Jaeger, Steffen Vanneste, and Jiří Friml. “Retromer Subunits VPS35A and VPS29 Mediate Prevacuolar Compartment (PVC) Function in Arabidopsis.” Molecular Plant. Cell Press, 2013. https://doi.org/10.1093/mp/sst044.","apa":"Nodzyński, T., Feraru, M., Hirsch, S., De Rycke, R., Nicuales, C., Van Leene, J., … Friml, J. (2013). Retromer subunits VPS35A and VPS29 mediate prevacuolar compartment (PVC) function in Arabidopsis. Molecular Plant. Cell Press. https://doi.org/10.1093/mp/sst044","ama":"Nodzyński T, Feraru M, Hirsch S, et al. Retromer subunits VPS35A and VPS29 mediate prevacuolar compartment (PVC) function in Arabidopsis. Molecular Plant. 2013;6(6):1849-1862. doi:10.1093/mp/sst044","ieee":"T. Nodzyński et al., “Retromer subunits VPS35A and VPS29 mediate prevacuolar compartment (PVC) function in Arabidopsis,” Molecular Plant, vol. 6, no. 6. Cell Press, pp. 1849–1862, 2013.","short":"T. Nodzyński, M. Feraru, S. Hirsch, R. De Rycke, C. Nicuales, J. Van Leene, G. De Jaeger, S. Vanneste, J. Friml, Molecular Plant 6 (2013) 1849–1862.","mla":"Nodzyński, Tomasz, et al. “Retromer Subunits VPS35A and VPS29 Mediate Prevacuolar Compartment (PVC) Function in Arabidopsis.” Molecular Plant, vol. 6, no. 6, Cell Press, 2013, pp. 1849–62, doi:10.1093/mp/sst044."},"date_updated":"2021-01-12T06:57:33Z","title":"Retromer subunits VPS35A and VPS29 mediate prevacuolar compartment (PVC) function in Arabidopsis","department":[{"_id":"JiFr"}],"publist_id":"4454","author":[{"last_name":"Nodzyński","full_name":"Nodzyński, Tomasz","first_name":"Tomasz"},{"first_name":"Murguel","full_name":"Feraru, Murguel","last_name":"Feraru"},{"last_name":"Hirsch","full_name":"Hirsch, Sibylle","first_name":"Sibylle"},{"first_name":"Riet","last_name":"De Rycke","full_name":"De Rycke, Riet"},{"full_name":"Nicuales, Claudiu","last_name":"Nicuales","first_name":"Claudiu"},{"last_name":"Van Leene","full_name":"Van Leene, Jelle","first_name":"Jelle"},{"first_name":"Geert","last_name":"De Jaeger","full_name":"De Jaeger, Geert"},{"first_name":"Steffen","last_name":"Vanneste","full_name":"Vanneste, Steffen"},{"full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí"}],"oa_version":"None","abstract":[{"text":"Intracellular protein routing is mediated by vesicular transport which is tightly regulated in eukaryotes. The protein and lipid homeostasis depends on coordinated delivery of de novo synthesized or recycled cargoes to the plasma membrane by exocytosis and their subsequent removal by rerouting them for recycling or degradation. Here, we report the characterization of protein affected trafficking 3 (pat3) mutant that we identified by an epifluorescence-based forward genetic screen for mutants defective in subcellular distribution of Arabidopsis auxin transporter PIN1–GFP. While pat3 displays largely normal plant morphology and development in nutrient-rich conditions, it shows strong ectopic intracellular accumulations of different plasma membrane cargoes in structures that resemble prevacuolar compartments (PVC) with an aberrant morphology. Genetic mapping revealed that pat3 is defective in vacuolar protein sorting 35A (VPS35A), a putative subunit of the retromer complex that mediates retrograde trafficking between the PVC and trans-Golgi network. Similarly, a mutant defective in another retromer subunit, vps29, shows comparable subcellular defects in PVC morphology and protein accumulation. Thus, our data provide evidence that the retromer components VPS35A and VPS29 are essential for normal PVC morphology and normal trafficking of plasma membrane proteins in plants. In addition, we show that, out of the three VPS35 retromer subunits present in Arabidopsis thaliana genome, the VPS35 homolog A plays a prevailing role in trafficking to the lytic vacuole, presenting another level of complexity in the retromer-dependent vacuolar sorting. ","lang":"eng"}],"month":"11","intvolume":" 6","scopus_import":1,"publisher":"Cell Press","quality_controlled":"1","day":"01","publication":"Molecular Plant","language":[{"iso":"eng"}],"year":"2013","publication_status":"published","issue":"6","date_published":"2013-11-01T00:00:00Z","doi":"10.1093/mp/sst044","volume":6,"date_created":"2018-12-11T11:57:44Z","page":"1849 - 1862"},{"publication_status":"published","file":[{"file_id":"5222","checksum":"3be71828b6c2ba9c90eb7056e3f7f57a","access_level":"open_access","relation":"main_file","content_type":"application/pdf","date_created":"2018-12-12T10:16:34Z","file_name":"IST-2015-393-v1+1_journal.pone.0070069.pdf","creator":"system","date_updated":"2020-07-14T12:45:41Z","file_size":9003465}],"language":[{"iso":"eng"}],"volume":8,"issue":"7","license":"https://creativecommons.org/licenses/by/4.0/","ec_funded":1,"abstract":[{"text":"Plant-specific PIN-formed (PIN) efflux transporters for the plant hormone auxin are required for tissue-specific directional auxin transport and cellular auxin homeostasis. The Arabidopsis PIN protein family has been shown to play important roles in developmental processes such as embryogenesis, organogenesis, vascular tissue differentiation, root meristem patterning and tropic growth. Here we analyzed roles of the less characterised Arabidopsis PIN6 auxin transporter. PIN6 is auxin-inducible and is expressed during multiple auxin-regulated developmental processes. Loss of pin6 function interfered with primary root growth and lateral root development. Misexpression of PIN6 affected auxin transport and interfered with auxin homeostasis in other growth processes such as shoot apical dominance, lateral root primordia development, adventitious root formation, root hair outgrowth and root waving. These changes in auxin-regulated growth correlated with a reduction in total auxin transport as well as with an altered activity of DR5-GUS auxin response reporter. Overall, the data indicate that PIN6 regulates auxin homeostasis during plant development.","lang":"eng"}],"oa_version":"Published Version","scopus_import":1,"month":"07","intvolume":" 8","date_updated":"2021-01-12T06:57:41Z","ddc":["580","570"],"department":[{"_id":"JiFr"},{"_id":"EvBe"}],"file_date_updated":"2020-07-14T12:45:41Z","_id":"2472","type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"status":"public","pubrep_id":"393","has_accepted_license":"1","year":"2013","day":"29","publication":"PLoS One","date_published":"2013-07-29T00:00:00Z","doi":"10.1371/journal.pone.0070069","date_created":"2018-12-11T11:57:52Z","publisher":"Public Library of Science","quality_controlled":"1","oa":1,"citation":{"chicago":"Cazzonelli, Christopher, Marleen Vanstraelen, Sibu Simon, Kuide Yin, Ashley Carron Arthur, Nazia Nisar, Gauri Tarle, et al. “Role of the Arabidopsis PIN6 Auxin Transporter in Auxin Homeostasis and Auxin-Mediated Development.” PLoS One. Public Library of Science, 2013. https://doi.org/10.1371/journal.pone.0070069.","ista":"Cazzonelli C, Vanstraelen M, Simon S, Yin K, Carron Arthur A, Nisar N, Tarle G, Cuttriss A, Searle I, Benková E, Mathesius U, Masle J, Friml J, Pogson B. 2013. Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and auxin-mediated development. PLoS One. 8(7), e70069.","mla":"Cazzonelli, Christopher, et al. “Role of the Arabidopsis PIN6 Auxin Transporter in Auxin Homeostasis and Auxin-Mediated Development.” PLoS One, vol. 8, no. 7, e70069, Public Library of Science, 2013, doi:10.1371/journal.pone.0070069.","apa":"Cazzonelli, C., Vanstraelen, M., Simon, S., Yin, K., Carron Arthur, A., Nisar, N., … Pogson, B. (2013). Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and auxin-mediated development. PLoS One. Public Library of Science. https://doi.org/10.1371/journal.pone.0070069","ama":"Cazzonelli C, Vanstraelen M, Simon S, et al. Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and auxin-mediated development. PLoS One. 2013;8(7). doi:10.1371/journal.pone.0070069","ieee":"C. Cazzonelli et al., “Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and auxin-mediated development,” PLoS One, vol. 8, no. 7. Public Library of Science, 2013.","short":"C. Cazzonelli, M. Vanstraelen, S. Simon, K. Yin, A. Carron Arthur, N. Nisar, G. Tarle, A. Cuttriss, I. Searle, E. Benková, U. Mathesius, J. Masle, J. Friml, B. Pogson, PLoS One 8 (2013)."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"first_name":"Christopher","full_name":"Cazzonelli, Christopher","last_name":"Cazzonelli"},{"last_name":"Vanstraelen","full_name":"Vanstraelen, Marleen","first_name":"Marleen"},{"full_name":"Simon, Sibu","orcid":"0000-0002-1998-6741","last_name":"Simon","first_name":"Sibu","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Kuide","full_name":"Yin, Kuide","last_name":"Yin"},{"full_name":"Carron Arthur, Ashley","last_name":"Carron Arthur","first_name":"Ashley"},{"full_name":"Nisar, Nazia","last_name":"Nisar","first_name":"Nazia"},{"full_name":"Tarle, Gauri","last_name":"Tarle","first_name":"Gauri"},{"full_name":"Cuttriss, Abby","last_name":"Cuttriss","first_name":"Abby"},{"first_name":"Iain","last_name":"Searle","full_name":"Searle, Iain"},{"first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","last_name":"Benková"},{"first_name":"Ulrike","last_name":"Mathesius","full_name":"Mathesius, Ulrike"},{"full_name":"Masle, Josette","last_name":"Masle","first_name":"Josette"},{"full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí"},{"last_name":"Pogson","full_name":"Pogson, Barry","first_name":"Barry"}],"publist_id":"4431","title":"Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and auxin-mediated development","article_number":"e70069","project":[{"_id":"253FCA6A-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","grant_number":"207362","name":"Hormonal cross-talk in plant organogenesis"},{"name":"Polarity and subcellular dynamics in plants","grant_number":"282300","_id":"25716A02-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"}]},{"title":"Overexpression of the auxin binding PROTEIN1 modulates PIN-dependent auxin transport in tobacco cells","publist_id":"4432","author":[{"last_name":"Čovanová","full_name":"Čovanová, Milada","first_name":"Milada"},{"first_name":"Michael","full_name":"Sauer, Michael","last_name":"Sauer"},{"first_name":"Jan","full_name":"Rychtář, Jan","last_name":"Rychtář"},{"first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","last_name":"Friml"},{"last_name":"Petrášek","full_name":"Petrášek, Jan","first_name":"Jan"},{"full_name":"Zažímalová, Eva","last_name":"Zažímalová","first_name":"Eva"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"short":"M. Čovanová, M. Sauer, J. Rychtář, J. Friml, J. Petrášek, E. Zažímalová, PLoS One 8 (2013).","ieee":"M. Čovanová, M. Sauer, J. Rychtář, J. Friml, J. Petrášek, and E. Zažímalová, “Overexpression of the auxin binding PROTEIN1 modulates PIN-dependent auxin transport in tobacco cells,” PLoS One, vol. 8, no. 7. Public Library of Science, 2013.","ama":"Čovanová M, Sauer M, Rychtář J, Friml J, Petrášek J, Zažímalová E. Overexpression of the auxin binding PROTEIN1 modulates PIN-dependent auxin transport in tobacco cells. PLoS One. 2013;8(7). doi:10.1371/journal.pone.0070050","apa":"Čovanová, M., Sauer, M., Rychtář, J., Friml, J., Petrášek, J., & Zažímalová, E. (2013). Overexpression of the auxin binding PROTEIN1 modulates PIN-dependent auxin transport in tobacco cells. PLoS One. Public Library of Science. https://doi.org/10.1371/journal.pone.0070050","mla":"Čovanová, Milada, et al. “Overexpression of the Auxin Binding PROTEIN1 Modulates PIN-Dependent Auxin Transport in Tobacco Cells.” PLoS One, vol. 8, no. 7, e70050, Public Library of Science, 2013, doi:10.1371/journal.pone.0070050.","ista":"Čovanová M, Sauer M, Rychtář J, Friml J, Petrášek J, Zažímalová E. 2013. Overexpression of the auxin binding PROTEIN1 modulates PIN-dependent auxin transport in tobacco cells. PLoS One. 8(7), e70050.","chicago":"Čovanová, Milada, Michael Sauer, Jan Rychtář, Jiří Friml, Jan Petrášek, and Eva Zažímalová. “Overexpression of the Auxin Binding PROTEIN1 Modulates PIN-Dependent Auxin Transport in Tobacco Cells.” PLoS One. Public Library of Science, 2013. https://doi.org/10.1371/journal.pone.0070050."},"article_number":"e70050","doi":"10.1371/journal.pone.0070050","date_published":"2013-07-23T00:00:00Z","date_created":"2018-12-11T11:57:51Z","day":"23","publication":"PLoS One","has_accepted_license":"1","year":"2013","quality_controlled":"1","publisher":"Public Library of Science","oa":1,"file_date_updated":"2020-07-14T12:45:41Z","department":[{"_id":"JiFr"}],"ddc":["570"],"date_updated":"2021-01-12T06:57:40Z","status":"public","pubrep_id":"413","type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"_id":"2470","issue":"7","volume":8,"file":[{"checksum":"2d47ef47616ef4de1d517d146548184e","file_id":"4681","content_type":"application/pdf","access_level":"open_access","relation":"main_file","date_created":"2018-12-12T10:08:21Z","file_name":"IST-2016-413-v1+1_journal.pone.0070050.pdf","date_updated":"2020-07-14T12:45:41Z","file_size":2294955,"creator":"system"}],"language":[{"iso":"eng"}],"publication_status":"published","month":"07","intvolume":" 8","scopus_import":1,"oa_version":"Published Version","abstract":[{"lang":"eng","text":"Background:Auxin binding protein 1 (ABP1) is a putative auxin receptor and its function is indispensable for plant growth and development. ABP1 has been shown to be involved in auxin-dependent regulation of cell division and expansion, in plasma-membrane-related processes such as changes in transmembrane potential, and in the regulation of clathrin-dependent endocytosis. However, the ABP1-regulated downstream pathway remains elusive.Methodology/Principal Findings:Using auxin transport assays and quantitative analysis of cellular morphology we show that ABP1 regulates auxin efflux from tobacco BY-2 cells. The overexpression of ABP1can counterbalance increased auxin efflux and auxin starvation phenotypes caused by the overexpression of PIN auxin efflux carrier. Relevant mechanism involves the ABP1-controlled vesicle trafficking processes, including positive regulation of endocytosis of PIN auxin efflux carriers, as indicated by fluorescence recovery after photobleaching (FRAP) and pharmacological manipulations.Conclusions/Significance:The findings indicate the involvement of ABP1 in control of rate of auxin transport across plasma membrane emphasizing the role of ABP1 in regulation of PIN activity at the plasma membrane, and highlighting the relevance of ABP1 for the formation of developmentally important, PIN-dependent auxin gradients."}]},{"department":[{"_id":"JiFr"}],"date_updated":"2021-01-12T06:59:51Z","status":"public","type":"journal_article","_id":"2808","issue":"3","volume":162,"language":[{"iso":"eng"}],"publication_status":"published","intvolume":" 162","month":"07","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3707547/","open_access":"1"}],"scopus_import":1,"oa_version":"Submitted Version","pmid":1,"abstract":[{"lang":"eng","text":"In order to establish a reference for analysis of the function of auxin and the auxin biosynthesis regulators SHORT INTERNODE/ STYLISH (SHI/STY) during Physcomitrella patens reproductive development, we have described male (antheridial) and female (archegonial) development in detail, including temporal and positional information of organ initiation. This has allowed us to define discrete stages of organ morphogenesis and to show that reproductive organ development in P. patens is highly organized and that organ phyllotaxis differs between vegetative and reproductive development. Using the PpSHI1 and PpSHI2 reporter and knockout lines, the auxin reporters GmGH3pro:GUS and PpPINApro:GFP-GUS, and the auxin-conjugating transgene PpSHI2pro:IAAL, we could show that the PpSHI genes, and by inference also auxin, play important roles for reproductive organ development in moss. The PpSHI genes are required for the apical opening of the reproductive organs, the final differentiation of the egg cell, and the progression of canal cells into a cell death program. The apical cells of the archegonium, the canal cells, and the egg cell are also sites of auxin responsiveness and are affected by reduced levels of active auxin, suggesting that auxin mediates PpSHI function in the reproductive organs."}],"title":"The moss physcomitrella patens reproductive organ development is highly organized, affected by the two SHI/STY genes and by the level of active auxin in the SHI/STY expression domain","external_id":{"pmid":["23669745"]},"author":[{"first_name":"Katarina","full_name":"Landberg, Katarina","last_name":"Landberg"},{"full_name":"Pederson, Eric","last_name":"Pederson","first_name":"Eric"},{"first_name":"Tom","full_name":"Viaene, Tom","last_name":"Viaene"},{"first_name":"Behruz","last_name":"Bozorg","full_name":"Bozorg, Behruz"},{"last_name":"Friml","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Jönsson, Henrik","last_name":"Jönsson","first_name":"Henrik"},{"full_name":"Thelander, Mattias","last_name":"Thelander","first_name":"Mattias"},{"first_name":"Eva","full_name":"Sundberg, Eva","last_name":"Sundberg"}],"publist_id":"4079","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"apa":"Landberg, K., Pederson, E., Viaene, T., Bozorg, B., Friml, J., Jönsson, H., … Sundberg, E. (2013). The moss physcomitrella patens reproductive organ development is highly organized, affected by the two SHI/STY genes and by the level of active auxin in the SHI/STY expression domain. Plant Physiology. American Society of Plant Biologists. https://doi.org/10.1104/pp.113.214023","ama":"Landberg K, Pederson E, Viaene T, et al. The moss physcomitrella patens reproductive organ development is highly organized, affected by the two SHI/STY genes and by the level of active auxin in the SHI/STY expression domain. Plant Physiology. 2013;162(3):1406-1419. doi:10.1104/pp.113.214023","ieee":"K. Landberg et al., “The moss physcomitrella patens reproductive organ development is highly organized, affected by the two SHI/STY genes and by the level of active auxin in the SHI/STY expression domain,” Plant Physiology, vol. 162, no. 3. American Society of Plant Biologists, pp. 1406–1419, 2013.","short":"K. Landberg, E. Pederson, T. Viaene, B. Bozorg, J. Friml, H. Jönsson, M. Thelander, E. Sundberg, Plant Physiology 162 (2013) 1406–1419.","mla":"Landberg, Katarina, et al. “The Moss Physcomitrella Patens Reproductive Organ Development Is Highly Organized, Affected by the Two SHI/STY Genes and by the Level of Active Auxin in the SHI/STY Expression Domain.” Plant Physiology, vol. 162, no. 3, American Society of Plant Biologists, 2013, pp. 1406–19, doi:10.1104/pp.113.214023.","ista":"Landberg K, Pederson E, Viaene T, Bozorg B, Friml J, Jönsson H, Thelander M, Sundberg E. 2013. The moss physcomitrella patens reproductive organ development is highly organized, affected by the two SHI/STY genes and by the level of active auxin in the SHI/STY expression domain. Plant Physiology. 162(3), 1406–1419.","chicago":"Landberg, Katarina, Eric Pederson, Tom Viaene, Behruz Bozorg, Jiří Friml, Henrik Jönsson, Mattias Thelander, and Eva Sundberg. “The Moss Physcomitrella Patens Reproductive Organ Development Is Highly Organized, Affected by the Two SHI/STY Genes and by the Level of Active Auxin in the SHI/STY Expression Domain.” Plant Physiology. American Society of Plant Biologists, 2013. https://doi.org/10.1104/pp.113.214023."},"date_created":"2018-12-11T11:59:42Z","doi":"10.1104/pp.113.214023","date_published":"2013-07-03T00:00:00Z","page":"1406 - 1419","publication":"Plant Physiology","day":"03","year":"2013","oa":1,"publisher":"American Society of Plant Biologists","quality_controlled":"1"},{"publisher":"American Society of Plant Biologists","quality_controlled":"1","oa":1,"day":"24","publication":"Plant Cell","year":"2013","date_published":"2013-04-24T00:00:00Z","doi":"10.1105/tpc.113.110353","date_created":"2018-12-11T11:59:46Z","page":"901 - 926","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"mla":"Remy, Estelle, et al. “A Major Facilitator Superfamily Transporter Plays a Dual Role in Polar Auxin Transport and Drought Stress Tolerance in Arabidopsis.” Plant Cell, vol. 25, no. 3, American Society of Plant Biologists, 2013, pp. 901–26, doi:10.1105/tpc.113.110353.","ama":"Remy E, Cabrito T, Baster P, et al. A major facilitator superfamily transporter plays a dual role in polar auxin transport and drought stress tolerance in Arabidopsis. Plant Cell. 2013;25(3):901-926. doi:10.1105/tpc.113.110353","apa":"Remy, E., Cabrito, T., Baster, P., Batista, R., Teixeira, M., Friml, J., … Duque, P. (2013). A major facilitator superfamily transporter plays a dual role in polar auxin transport and drought stress tolerance in Arabidopsis. Plant Cell. American Society of Plant Biologists. https://doi.org/10.1105/tpc.113.110353","ieee":"E. Remy et al., “A major facilitator superfamily transporter plays a dual role in polar auxin transport and drought stress tolerance in Arabidopsis,” Plant Cell, vol. 25, no. 3. American Society of Plant Biologists, pp. 901–926, 2013.","short":"E. Remy, T. Cabrito, P. Baster, R. Batista, M. Teixeira, J. Friml, I. Sá Correia, P. Duque, Plant Cell 25 (2013) 901–926.","chicago":"Remy, Estelle, Tânia Cabrito, Pawel Baster, Rita Batista, Miguel Teixeira, Jiří Friml, Isabel Sá Correia, and Paula Duque. “A Major Facilitator Superfamily Transporter Plays a Dual Role in Polar Auxin Transport and Drought Stress Tolerance in Arabidopsis.” Plant Cell. American Society of Plant Biologists, 2013. https://doi.org/10.1105/tpc.113.110353.","ista":"Remy E, Cabrito T, Baster P, Batista R, Teixeira M, Friml J, Sá Correia I, Duque P. 2013. A major facilitator superfamily transporter plays a dual role in polar auxin transport and drought stress tolerance in Arabidopsis. Plant Cell. 25(3), 901–926."},"title":"A major facilitator superfamily transporter plays a dual role in polar auxin transport and drought stress tolerance in Arabidopsis","author":[{"first_name":"Estelle","full_name":"Remy, Estelle","last_name":"Remy"},{"full_name":"Cabrito, Tânia","last_name":"Cabrito","first_name":"Tânia"},{"first_name":"Pawel","id":"3028BD74-F248-11E8-B48F-1D18A9856A87","last_name":"Baster","full_name":"Baster, Pawel"},{"full_name":"Batista, Rita","last_name":"Batista","first_name":"Rita"},{"first_name":"Miguel","last_name":"Teixeira","full_name":"Teixeira, Miguel"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí","last_name":"Friml","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí"},{"last_name":"Sá Correia","full_name":"Sá Correia, Isabel","first_name":"Isabel"},{"full_name":"Duque, Paula","last_name":"Duque","first_name":"Paula"}],"publist_id":"3980","external_id":{"pmid":["23524662"]},"pmid":1,"oa_version":"Submitted Version","abstract":[{"lang":"eng","text":"Many key aspects of plant development are regulated by the polarized transport of the phytohormone auxin. Cellular auxin efflux, the rate-limiting step in this process, has been shown to rely on the coordinated action of PIN-formed (PIN) and B-type ATP binding cassette (ABCB) carriers. Here, we report that polar auxin transport in the Arabidopsis thaliana root also requires the action of a Major Facilitator Superfamily (MFS) transporter, Zinc-Induced Facilitator-Like 1 (ZIFL1). Sequencing, promoter-reporter, and fluorescent protein fusion experiments indicate that the full-length ZIFL1.1 protein and a truncated splice isoform, ZIFL1.3, localize to the tonoplast of root cells and the plasma membrane of leaf stomatal guard cells, respectively. Using reverse genetics, we show that the ZIFL1.1 transporter regulates various root auxin-related processes, while the ZIFL1.3 isoform mediates drought tolerance by regulating stomatal closure. Auxin transport and immunolocalization assays demonstrate that ZIFL1.1 indirectly modulates cellular auxin efflux during shootward auxin transport at the root tip, likely by regulating plasma membrane PIN2 abundance. Finally, heterologous expression in yeast revealed that ZIFL1.1 and ZIFL1.3 share H+-coupled K+ transport activity. Thus, by determining the subcellular and tissue distribution of two isoforms, alternative splicing dictates a dual function for the ZIFL1 transporter. We propose that this MFS carrier regulates stomatal movements and polar auxin transport by modulating potassium and proton fluxes in Arabidopsis cells."}],"month":"04","intvolume":" 25","scopus_import":1,"main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3634696/"}],"language":[{"iso":"eng"}],"publication_status":"published","volume":25,"issue":"3","_id":"2821","status":"public","type":"journal_article","date_updated":"2021-01-12T06:59:57Z","department":[{"_id":"JiFr"}]},{"citation":{"chicago":"Du, Yunlong, Ricardo Tejos, Martina Beck, Ellie Himschoot, Hongjiang Li, Silke Robatzek, Steffen Vanneste, and Jiří Friml. “Salicylic Acid Interferes with Clathrin-Mediated Endocytic Protein Trafficking.” PNAS. National Academy of Sciences, 2013. https://doi.org/10.1073/pnas.1220205110.","ista":"Du Y, Tejos R, Beck M, Himschoot E, Li H, Robatzek S, Vanneste S, Friml J. 2013. Salicylic acid interferes with clathrin-mediated endocytic protein trafficking. PNAS. 110(19), 7946–7951.","mla":"Du, Yunlong, et al. “Salicylic Acid Interferes with Clathrin-Mediated Endocytic Protein Trafficking.” PNAS, vol. 110, no. 19, National Academy of Sciences, 2013, pp. 7946–51, doi:10.1073/pnas.1220205110.","ama":"Du Y, Tejos R, Beck M, et al. Salicylic acid interferes with clathrin-mediated endocytic protein trafficking. PNAS. 2013;110(19):7946-7951. doi:10.1073/pnas.1220205110","apa":"Du, Y., Tejos, R., Beck, M., Himschoot, E., Li, H., Robatzek, S., … Friml, J. (2013). Salicylic acid interferes with clathrin-mediated endocytic protein trafficking. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1220205110","ieee":"Y. Du et al., “Salicylic acid interferes with clathrin-mediated endocytic protein trafficking,” PNAS, vol. 110, no. 19. National Academy of Sciences, pp. 7946–7951, 2013.","short":"Y. Du, R. Tejos, M. Beck, E. Himschoot, H. Li, S. Robatzek, S. Vanneste, J. Friml, PNAS 110 (2013) 7946–7951."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","external_id":{"pmid":["23613581"]},"publist_id":"3972","author":[{"full_name":"Du, Yunlong","last_name":"Du","first_name":"Yunlong"},{"first_name":"Ricardo","last_name":"Tejos","full_name":"Tejos, Ricardo"},{"full_name":"Beck, Martina","last_name":"Beck","first_name":"Martina"},{"full_name":"Himschoot, Ellie","last_name":"Himschoot","first_name":"Ellie"},{"id":"33CA54A6-F248-11E8-B48F-1D18A9856A87","first_name":"Hongjiang","last_name":"Li","orcid":"0000-0001-5039-9660","full_name":"Li, Hongjiang"},{"first_name":"Silke","full_name":"Robatzek, Silke","last_name":"Robatzek"},{"full_name":"Vanneste, Steffen","last_name":"Vanneste","first_name":"Steffen"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí","last_name":"Friml","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596"}],"title":"Salicylic acid interferes with clathrin-mediated endocytic protein trafficking","project":[{"_id":"2574781E-B435-11E9-9278-68D0E5697425","name":"Koerber Prize 2010"}],"year":"2013","publication":"PNAS","day":"07","page":"7946 - 7951","date_created":"2018-12-11T11:59:48Z","doi":"10.1073/pnas.1220205110","date_published":"2013-05-07T00:00:00Z","oa":1,"quality_controlled":"1","publisher":"National Academy of Sciences","date_updated":"2021-01-12T06:59:59Z","department":[{"_id":"JiFr"}],"_id":"2827","type":"journal_article","status":"public","publication_status":"published","language":[{"iso":"eng"}],"volume":110,"issue":"19","abstract":[{"lang":"eng","text":"Removal of cargos from the cell surface via endocytosis is an efficient mechanism to regulate activities of plasma membrane (PM)-resident proteins, such as receptors or transporters. Salicylic acid (SA) is an important plant hormone that is traditionally associated with pathogen defense. Here, we describe an unanticipated effect of SA on subcellular endocytic cycling of proteins. Both exogenous treatments and endogenously enhanced SA levels repressed endocytosis of different PM proteins. The SA effect on endocytosis did not involve transcription or known components of the SA signaling pathway for transcriptional regulation. SA likely targets an endocytic mechanism that involves the coat protein clathrin, because SA interfered with the clathrin incidence at the PM and clathrin-deficient mutants were less sensitive to the impact of SA on the auxin distribution and root bending during the gravitropic response. By contrast, SA did not affect the ligand-induced endocytosis of the FLAGELLIN SENSING2 (FLS2) receptor during pathogen responses. Our data suggest that the established SA impact on transcription in plant immunity and the nontranscriptional effect of SA on clathrin-mediated endocytosis are independent mechanisms by which SA regulates distinct aspects of plant physiology."}],"oa_version":"Submitted Version","pmid":1,"main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3651428/"}],"scopus_import":1,"intvolume":" 110","month":"05"},{"date_updated":"2021-01-12T07:00:03Z","ddc":["570"],"department":[{"_id":"JiFr"}],"file_date_updated":"2020-07-14T12:45:50Z","_id":"2832","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"type":"journal_article","pubrep_id":"411","status":"public","publication_status":"published","language":[{"iso":"eng"}],"file":[{"file_id":"4957","checksum":"050237d6c53e8d1601b26808ee1dd6d8","relation":"main_file","access_level":"open_access","content_type":"application/pdf","file_name":"IST-2016-411-v1+1_journal.pgen.1003540.pdf","date_created":"2018-12-12T10:12:39Z","creator":"system","file_size":3813091,"date_updated":"2020-07-14T12:45:50Z"}],"ec_funded":1,"issue":"5","volume":9,"abstract":[{"text":"PIN-FORMED (PIN) proteins localize asymmetrically at the plasma membrane and mediate intercellular polar transport of the plant hormone auxin that is crucial for a multitude of developmental processes in plants. PIN localization is under extensive control by environmental or developmental cues, but mechanisms regulating PIN localization are not fully understood. Here we show that early endosomal components ARF GEF BEN1 and newly identified Sec1/Munc18 family protein BEN2 are involved in distinct steps of early endosomal trafficking. BEN1 and BEN2 are collectively required for polar PIN localization, for their dynamic repolarization, and consequently for auxin activity gradient formation and auxin-related developmental processes including embryonic patterning, organogenesis, and vasculature venation patterning. These results show that early endosomal trafficking is crucial for cell polarity and auxin-dependent regulation of plant architecture.","lang":"eng"}],"oa_version":"Published Version","scopus_import":1,"intvolume":" 9","month":"05","citation":{"chicago":"Tanaka, Hirokazu, Saeko Kitakura, Hana Rakusová, Tomohiro Uemura, Mugurel Feraru, Riet De Rycke, Stéphanie Robert, Tatsuo Kakimoto, and Jiří Friml. “Cell Polarity and Patterning by PIN Trafficking through Early Endosomal Compartments in Arabidopsis Thaliana.” PLoS Genetics. Public Library of Science, 2013. https://doi.org/10.1371/journal.pgen.1003540.","ista":"Tanaka H, Kitakura S, Rakusová H, Uemura T, Feraru M, De Rycke R, Robert S, Kakimoto T, Friml J. 2013. Cell polarity and patterning by PIN trafficking through early endosomal compartments in arabidopsis thaliana. PLoS Genetics. 9(5), e1003540.","mla":"Tanaka, Hirokazu, et al. “Cell Polarity and Patterning by PIN Trafficking through Early Endosomal Compartments in Arabidopsis Thaliana.” PLoS Genetics, vol. 9, no. 5, e1003540, Public Library of Science, 2013, doi:10.1371/journal.pgen.1003540.","apa":"Tanaka, H., Kitakura, S., Rakusová, H., Uemura, T., Feraru, M., De Rycke, R., … Friml, J. (2013). Cell polarity and patterning by PIN trafficking through early endosomal compartments in arabidopsis thaliana. PLoS Genetics. Public Library of Science. https://doi.org/10.1371/journal.pgen.1003540","ama":"Tanaka H, Kitakura S, Rakusová H, et al. Cell polarity and patterning by PIN trafficking through early endosomal compartments in arabidopsis thaliana. PLoS Genetics. 2013;9(5). doi:10.1371/journal.pgen.1003540","short":"H. Tanaka, S. Kitakura, H. Rakusová, T. Uemura, M. Feraru, R. De Rycke, S. Robert, T. Kakimoto, J. Friml, PLoS Genetics 9 (2013).","ieee":"H. Tanaka et al., “Cell polarity and patterning by PIN trafficking through early endosomal compartments in arabidopsis thaliana,” PLoS Genetics, vol. 9, no. 5. Public Library of Science, 2013."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"first_name":"Hirokazu","last_name":"Tanaka","full_name":"Tanaka, Hirokazu"},{"first_name":"Saeko","last_name":"Kitakura","full_name":"Kitakura, Saeko"},{"last_name":"Rakusová","full_name":"Rakusová, Hana","first_name":"Hana"},{"first_name":"Tomohiro","last_name":"Uemura","full_name":"Uemura, Tomohiro"},{"first_name":"Mugurel","full_name":"Feraru, Mugurel","last_name":"Feraru"},{"first_name":"Riet","full_name":"De Rycke, Riet","last_name":"De Rycke"},{"full_name":"Robert, Stéphanie","last_name":"Robert","first_name":"Stéphanie"},{"last_name":"Kakimoto","full_name":"Kakimoto, Tatsuo","first_name":"Tatsuo"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí"}],"publist_id":"3967","title":"Cell polarity and patterning by PIN trafficking through early endosomal compartments in arabidopsis thaliana","article_number":"e1003540","project":[{"grant_number":"282300","name":"Polarity and subcellular dynamics in plants","call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425"}],"year":"2013","has_accepted_license":"1","publication":"PLoS Genetics","day":"05","date_created":"2018-12-11T11:59:50Z","doi":"10.1371/journal.pgen.1003540","date_published":"2013-05-05T00:00:00Z","oa":1,"quality_controlled":"1","publisher":"Public Library of Science"},{"status":"public","type":"journal_article","_id":"2835","department":[{"_id":"JiFr"}],"date_updated":"2021-01-12T07:00:05Z","intvolume":" 162","month":"06","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3668084/","open_access":"1"}],"scopus_import":1,"pmid":1,"oa_version":"Submitted Version","abstract":[{"lang":"eng","text":"The phytohormone auxin regulates virtually every aspect of plant development. To identify new genes involved in auxin activity, a genetic screen was performed for Arabidopsis (Arabidopsis thaliana) mutants with altered expression of the auxin-responsive reporter DR5rev:GFP. One of the mutants recovered in the screen, designated as weak auxin response3 (wxr3), exhibits much lower DR5rev:GFP expression when treated with the synthetic auxin 2,4-dichlorophenoxyacetic acid and displays severe defects in root development. The wxr3 mutant decreases polar auxin transport and results in a disruption of the asymmetric auxin distribution. The levels of the auxin transporters AUXIN1 and PIN-FORMED are dramatically reduced in the wxr3 root tip. Molecular analyses demonstrate that WXR3 is ROOT ULTRAVIOLET B-SENSITIVE1 (RUS1), a member of the conserved Domain of Unknown Function647 protein family found in diverse eukaryotic organisms. Our data suggest that RUS1/WXR3 plays an essential role in the regulation of polar auxin transport by maintaining the proper level of auxin transporters on the plasma membrane."}],"volume":162,"issue":"2","language":[{"iso":"eng"}],"publication_status":"published","title":"Root ultraviolet b-sensitive1/weak auxin response3 is essential for polar auxin transport in arabidopsis","external_id":{"pmid":["23580592"]},"author":[{"first_name":"Hong","full_name":"Yu, Hong","last_name":"Yu"},{"first_name":"Michael","full_name":"Karampelias, Michael","last_name":"Karampelias"},{"first_name":"Stéphanie","last_name":"Robert","full_name":"Robert, Stéphanie"},{"last_name":"Peer","full_name":"Peer, Wendy","first_name":"Wendy"},{"first_name":"Ranjan","full_name":"Swarup, Ranjan","last_name":"Swarup"},{"last_name":"Ye","full_name":"Ye, Songqing","first_name":"Songqing"},{"first_name":"Lei","last_name":"Ge","full_name":"Ge, Lei"},{"first_name":"Jerry","full_name":"Cohen, Jerry","last_name":"Cohen"},{"full_name":"Murphy, Angus","last_name":"Murphy","first_name":"Angus"},{"first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596"},{"first_name":"Mark","full_name":"Estelle, Mark","last_name":"Estelle"}],"publist_id":"3964","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"chicago":"Yu, Hong, Michael Karampelias, Stéphanie Robert, Wendy Peer, Ranjan Swarup, Songqing Ye, Lei Ge, et al. “Root Ultraviolet B-Sensitive1/Weak Auxin Response3 Is Essential for Polar Auxin Transport in Arabidopsis.” Plant Physiology. American Society of Plant Biologists, 2013. https://doi.org/10.1104/pp.113.217018.","ista":"Yu H, Karampelias M, Robert S, Peer W, Swarup R, Ye S, Ge L, Cohen J, Murphy A, Friml J, Estelle M. 2013. Root ultraviolet b-sensitive1/weak auxin response3 is essential for polar auxin transport in arabidopsis. Plant Physiology. 162(2), 965–976.","mla":"Yu, Hong, et al. “Root Ultraviolet B-Sensitive1/Weak Auxin Response3 Is Essential for Polar Auxin Transport in Arabidopsis.” Plant Physiology, vol. 162, no. 2, American Society of Plant Biologists, 2013, pp. 965–76, doi:10.1104/pp.113.217018.","short":"H. Yu, M. Karampelias, S. Robert, W. Peer, R. Swarup, S. Ye, L. Ge, J. Cohen, A. Murphy, J. Friml, M. Estelle, Plant Physiology 162 (2013) 965–976.","ieee":"H. Yu et al., “Root ultraviolet b-sensitive1/weak auxin response3 is essential for polar auxin transport in arabidopsis,” Plant Physiology, vol. 162, no. 2. American Society of Plant Biologists, pp. 965–976, 2013.","apa":"Yu, H., Karampelias, M., Robert, S., Peer, W., Swarup, R., Ye, S., … Estelle, M. (2013). Root ultraviolet b-sensitive1/weak auxin response3 is essential for polar auxin transport in arabidopsis. Plant Physiology. American Society of Plant Biologists. https://doi.org/10.1104/pp.113.217018","ama":"Yu H, Karampelias M, Robert S, et al. Root ultraviolet b-sensitive1/weak auxin response3 is essential for polar auxin transport in arabidopsis. Plant Physiology. 2013;162(2):965-976. doi:10.1104/pp.113.217018"},"oa":1,"publisher":"American Society of Plant Biologists","quality_controlled":"1","date_created":"2018-12-11T11:59:51Z","date_published":"2013-06-01T00:00:00Z","doi":"10.1104/pp.113.217018","page":"965 - 976","publication":"Plant Physiology","day":"01","year":"2013"},{"_id":"2844","type":"journal_article","status":"public","project":[{"call_identifier":"FP7","_id":"253FCA6A-B435-11E9-9278-68D0E5697425","grant_number":"207362","name":"Hormonal cross-talk in plant organogenesis"}],"date_updated":"2021-01-12T07:00:10Z","citation":{"ista":"Rosquete M, von Wangenheim D, Marhavý P, Barbez E, Stelzer E, Benková E, Maizel A, Kleine Vehn J. 2013. An auxin transport mechanism restricts positive orthogravitropism in lateral roots. Current Biology. 23(9), 817–822.","chicago":"Rosquete, Michel, Daniel von Wangenheim, Peter Marhavý, Elke Barbez, Ernst Stelzer, Eva Benková, Alexis Maizel, and Jürgen Kleine Vehn. “An Auxin Transport Mechanism Restricts Positive Orthogravitropism in Lateral Roots.” Current Biology. Cell Press, 2013. https://doi.org/10.1016/j.cub.2013.03.064.","ieee":"M. Rosquete et al., “An auxin transport mechanism restricts positive orthogravitropism in lateral roots,” Current Biology, vol. 23, no. 9. Cell Press, pp. 817–822, 2013.","short":"M. Rosquete, D. von Wangenheim, P. Marhavý, E. Barbez, E. Stelzer, E. Benková, A. Maizel, J. Kleine Vehn, Current Biology 23 (2013) 817–822.","apa":"Rosquete, M., von Wangenheim, D., Marhavý, P., Barbez, E., Stelzer, E., Benková, E., … Kleine Vehn, J. (2013). An auxin transport mechanism restricts positive orthogravitropism in lateral roots. Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2013.03.064","ama":"Rosquete M, von Wangenheim D, Marhavý P, et al. An auxin transport mechanism restricts positive orthogravitropism in lateral roots. Current Biology. 2013;23(9):817-822. doi:10.1016/j.cub.2013.03.064","mla":"Rosquete, Michel, et al. “An Auxin Transport Mechanism Restricts Positive Orthogravitropism in Lateral Roots.” Current Biology, vol. 23, no. 9, Cell Press, 2013, pp. 817–22, doi:10.1016/j.cub.2013.03.064."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publist_id":"3950","author":[{"full_name":"Rosquete, Michel","last_name":"Rosquete","first_name":"Michel"},{"last_name":"Von Wangenheim","orcid":"0000-0002-6862-1247","full_name":"Von Wangenheim, Daniel","first_name":"Daniel","id":"49E91952-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Peter","id":"3F45B078-F248-11E8-B48F-1D18A9856A87","last_name":"Marhavy","orcid":"0000-0001-5227-5741","full_name":"Marhavy, Peter"},{"full_name":"Barbez, Elke","last_name":"Barbez","first_name":"Elke"},{"last_name":"Stelzer","full_name":"Stelzer, Ernst","first_name":"Ernst"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","last_name":"Benková","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva"},{"last_name":"Maizel","full_name":"Maizel, Alexis","first_name":"Alexis"},{"last_name":"Kleine Vehn","full_name":"Kleine Vehn, Jürgen","first_name":"Jürgen"}],"department":[{"_id":"JiFr"},{"_id":"EvBe"}],"title":"An auxin transport mechanism restricts positive orthogravitropism in lateral roots","abstract":[{"text":"As soon as a seed germinates, plant growth relates to gravity to ensure that the root penetrates the soil and the shoot expands aerially. Whereas mechanisms of positive and negative orthogravitropism of primary roots and shoots are relatively well understood [1-3], lateral organs often show more complex growth behavior [4]. Lateral roots (LRs) seemingly suppress positive gravitropic growth and show a defined gravitropic set-point angle (GSA) that allows radial expansion of the root system (plagiotropism) [3, 4]. Despite its eminent importance for root architecture, it so far remains completely unknown how lateral organs partially suppress positive orthogravitropism. Here we show that the phytohormone auxin steers GSA formation and limits positive orthogravitropism in LR. Low and high auxin levels/signaling lead to radial or axial root systems, respectively. At a cellular level, it is the auxin transport-dependent regulation of asymmetric growth in the elongation zone that determines GSA. Our data suggest that strong repression of PIN4/PIN7 and transient PIN3 expression limit auxin redistribution in young LR columella cells. We conclude that PIN activity, by temporally limiting the asymmetric auxin fluxes in the tip of LRs, induces transient, differential growth responses in the elongation zone and, consequently, controls root architecture.","lang":"eng"}],"oa_version":"None","scopus_import":1,"publisher":"Cell Press","quality_controlled":"1","intvolume":" 23","month":"05","year":"2013","publication_status":"published","language":[{"iso":"eng"}],"publication":"Current Biology","day":"06","page":"817 - 822","date_created":"2018-12-11T11:59:53Z","ec_funded":1,"doi":"10.1016/j.cub.2013.03.064","volume":23,"date_published":"2013-05-06T00:00:00Z","issue":"9"},{"status":"public","type":"journal_article","_id":"2883","department":[{"_id":"JiFr"}],"date_updated":"2021-01-12T07:00:28Z","month":"01","intvolume":" 25","scopus_import":1,"main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584535/"}],"pmid":1,"oa_version":"Submitted Version","abstract":[{"text":"Plant architecture is influenced by the polar, cell-to-cell transport of auxin that is primarily provided and regulated by plasma membrane efflux catalysts of the PIN-FORMED and B family of ABC transporter (ABCB) classes. The latter were shown to require the functionality of the FK506 binding protein42 TWISTED DWARF1 (TWD1), although underlying mechanisms are unclear. By genetic manipulation of TWD1 expression, we show here that TWD1 affects shootward root auxin reflux and, thus, downstream developmental traits, such as epidermal twisting and gravitropism of the root. Using immunological assays, we demonstrate a predominant lateral, mainly outward-facing, plasma membrane location for TWD1 in the root epidermis characterized by the lateral marker ABC transporter G36/PLEIOTROPIC DRUG-RESISTANCE8/PENETRATION3. At these epidermal plasma membrane domains, TWD1 colocalizes with nonpolar ABCB1. In planta bioluminescence resonance energy transfer analysis was used to verify specific ABC transporter B1 (ABCB1)-TWD1 interaction. Our data support a model in which TWD1 promotes lateral ABCB-mediated auxin efflux via protein-protein interaction at the plasma membrane, minimizing reflux from the root apoplast into the cytoplasm.","lang":"eng"}],"volume":25,"issue":"1","language":[{"iso":"eng"}],"publication_status":"published","title":"Arabidopsis TWISTED DWARF1 functionally interacts with auxin exporter ABCB1 on the root plasma membrane","author":[{"last_name":"Wang","full_name":"Wang, Bangjun","first_name":"Bangjun"},{"first_name":"Aurélien","last_name":"Bailly","full_name":"Bailly, Aurélien"},{"first_name":"Marta","last_name":"Zwiewk","full_name":"Zwiewk, Marta"},{"first_name":"Sina","full_name":"Henrichs, Sina","last_name":"Henrichs"},{"last_name":"Azzarello","full_name":"Azzarello, Elisa","first_name":"Elisa"},{"last_name":"Mancuso","full_name":"Mancuso, Stefano","first_name":"Stefano"},{"first_name":"Masayoshi","full_name":"Maeshima, Masayoshi","last_name":"Maeshima"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí"},{"first_name":"Alexander","last_name":"Schulz","full_name":"Schulz, Alexander"},{"first_name":"Markus","last_name":"Geisler","full_name":"Geisler, Markus"}],"publist_id":"3878","external_id":{"pmid":["23321285"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"ista":"Wang B, Bailly A, Zwiewk M, Henrichs S, Azzarello E, Mancuso S, Maeshima M, Friml J, Schulz A, Geisler M. 2013. Arabidopsis TWISTED DWARF1 functionally interacts with auxin exporter ABCB1 on the root plasma membrane. Plant Cell. 25(1), 202–214.","chicago":"Wang, Bangjun, Aurélien Bailly, Marta Zwiewk, Sina Henrichs, Elisa Azzarello, Stefano Mancuso, Masayoshi Maeshima, Jiří Friml, Alexander Schulz, and Markus Geisler. “Arabidopsis TWISTED DWARF1 Functionally Interacts with Auxin Exporter ABCB1 on the Root Plasma Membrane.” Plant Cell. American Society of Plant Biologists, 2013. https://doi.org/10.1105/tpc.112.105999.","ieee":"B. Wang et al., “Arabidopsis TWISTED DWARF1 functionally interacts with auxin exporter ABCB1 on the root plasma membrane,” Plant Cell, vol. 25, no. 1. American Society of Plant Biologists, pp. 202–214, 2013.","short":"B. Wang, A. Bailly, M. Zwiewk, S. Henrichs, E. Azzarello, S. Mancuso, M. Maeshima, J. Friml, A. Schulz, M. Geisler, Plant Cell 25 (2013) 202–214.","ama":"Wang B, Bailly A, Zwiewk M, et al. Arabidopsis TWISTED DWARF1 functionally interacts with auxin exporter ABCB1 on the root plasma membrane. Plant Cell. 2013;25(1):202-214. doi:10.1105/tpc.112.105999","apa":"Wang, B., Bailly, A., Zwiewk, M., Henrichs, S., Azzarello, E., Mancuso, S., … Geisler, M. (2013). Arabidopsis TWISTED DWARF1 functionally interacts with auxin exporter ABCB1 on the root plasma membrane. Plant Cell. American Society of Plant Biologists. https://doi.org/10.1105/tpc.112.105999","mla":"Wang, Bangjun, et al. “Arabidopsis TWISTED DWARF1 Functionally Interacts with Auxin Exporter ABCB1 on the Root Plasma Membrane.” Plant Cell, vol. 25, no. 1, American Society of Plant Biologists, 2013, pp. 202–14, doi:10.1105/tpc.112.105999."},"publisher":"American Society of Plant Biologists","quality_controlled":"1","oa":1,"acknowledgement":"We would thank Vincent Vincenzetti and Laurence Charrier for excellent technical assistance, A. von Arnim for the donation of BRET vectors, E. Spalding for TWD1-CFP, TWD1-CFP/29-1-GFP/ER-YFP, and ABCB4-GFP lines, M. Palmgren for discussion and support, and E. Martinoia for TT12 cDNA, support, and mentorship. Imaging data were partially collected at the Center for Advanced Bioimaging, University of Copenhagen, Denmark. This work was supported by grants from the Novartis Foundation (to M.G.), from the Danish Research School for Biotechnology (to M.G. and A.S.), from the Forschungskredit of the University of Zurich (to A.B.), from the Pool de Recherche of the University of Fribourg (to M.G.), and from the Swiss National Funds (to M.G.). M.G. dedicates this work to his father, who passed away during the resubmission process.","date_published":"2013-01-01T00:00:00Z","doi":"10.1105/tpc.112.105999","date_created":"2018-12-11T12:00:08Z","page":"202 - 214","day":"01","publication":"Plant Cell","year":"2013"},{"issue":"9","volume":110,"language":[{"iso":"eng"}],"publication_status":"published","month":"02","intvolume":" 110","scopus_import":1,"main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3587205/"}],"oa_version":"Submitted Version","pmid":1,"abstract":[{"text":"Gravitropic bending of plant organs is mediated by an asymmetric signaling of the plant hormone auxin between the upper and lower side of the respective organ. Here, we show that also another plant hormone, gibberellic acid (GA), shows asymmetric action during gravitropic responses. Immunodetection using an antibody against GA and monitoring GA signaling output by downstream degradation of DELLA proteins revealed an asymmetric GA distribution and response with the maximum at the lower side of gravistimulated roots. Genetic or pharmacological manipulation of GA levels or response affects gravity-mediated auxin redistribution and root bending response. The higher GA levels at the lower side of the root correlate with increased amounts of PIN-FORMED2 (PIN2) auxin transporter at the plasma membrane. The observed increase in PIN2 stability is caused by a specific GA effect on trafficking of PIN proteins to lytic vacuoles that presumably occurs downstream of brefeldin A-sensitive endosomes. Our results suggest that asymmetric auxin distribution instructive for gravity-induced differential growth is consolidated by the asymmetric action of GA that stabilizes the PIN-dependent auxin stream along the lower side of gravistimulated roots.","lang":"eng"}],"department":[{"_id":"JiFr"}],"date_updated":"2021-01-12T07:00:27Z","status":"public","type":"journal_article","_id":"2882","date_published":"2013-02-26T00:00:00Z","doi":"10.1073/pnas.1300107110","date_created":"2018-12-11T12:00:07Z","page":"3627 - 3632","day":"26","publication":"PNAS","year":"2013","quality_controlled":"1","publisher":"National Academy of Sciences","oa":1,"title":"Asymmetric gibberellin signaling regulates vacuolar trafficking of PIN auxin transporters during root gravitropism","author":[{"last_name":"Löfke","full_name":"Löfke, Christian","first_name":"Christian"},{"full_name":"Zwiewka, Marta","last_name":"Zwiewka","first_name":"Marta"},{"first_name":"Ingo","last_name":"Heilmann","full_name":"Heilmann, Ingo"},{"first_name":"Marc","last_name":"Van Montagu","full_name":"Van Montagu, Marc"},{"first_name":"Thomas","full_name":"Teichmann, Thomas","last_name":"Teichmann"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí"}],"publist_id":"3879","external_id":{"pmid":["23391733"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"mla":"Löfke, Christian, et al. “Asymmetric Gibberellin Signaling Regulates Vacuolar Trafficking of PIN Auxin Transporters during Root Gravitropism.” PNAS, vol. 110, no. 9, National Academy of Sciences, 2013, pp. 3627–32, doi:10.1073/pnas.1300107110.","ama":"Löfke C, Zwiewka M, Heilmann I, Van Montagu M, Teichmann T, Friml J. Asymmetric gibberellin signaling regulates vacuolar trafficking of PIN auxin transporters during root gravitropism. PNAS. 2013;110(9):3627-3632. doi:10.1073/pnas.1300107110","apa":"Löfke, C., Zwiewka, M., Heilmann, I., Van Montagu, M., Teichmann, T., & Friml, J. (2013). Asymmetric gibberellin signaling regulates vacuolar trafficking of PIN auxin transporters during root gravitropism. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1300107110","ieee":"C. Löfke, M. Zwiewka, I. Heilmann, M. Van Montagu, T. Teichmann, and J. Friml, “Asymmetric gibberellin signaling regulates vacuolar trafficking of PIN auxin transporters during root gravitropism,” PNAS, vol. 110, no. 9. National Academy of Sciences, pp. 3627–3632, 2013.","short":"C. Löfke, M. Zwiewka, I. Heilmann, M. Van Montagu, T. Teichmann, J. Friml, PNAS 110 (2013) 3627–3632.","chicago":"Löfke, Christian, Marta Zwiewka, Ingo Heilmann, Marc Van Montagu, Thomas Teichmann, and Jiří Friml. “Asymmetric Gibberellin Signaling Regulates Vacuolar Trafficking of PIN Auxin Transporters during Root Gravitropism.” PNAS. National Academy of Sciences, 2013. https://doi.org/10.1073/pnas.1300107110.","ista":"Löfke C, Zwiewka M, Heilmann I, Van Montagu M, Teichmann T, Friml J. 2013. Asymmetric gibberellin signaling regulates vacuolar trafficking of PIN auxin transporters during root gravitropism. PNAS. 110(9), 3627–3632."}},{"citation":{"mla":"Baster, Pawel, et al. “SCF^TIR1 AFB-Auxin Signalling Regulates PIN Vacuolar Trafficking and Auxin Fluxes during Root Gravitropism.” EMBO Journal, vol. 32, no. 2, Wiley-Blackwell, 2013, pp. 260–74, doi:10.1038/emboj.2012.310.","apa":"Baster, P., Robert, S., Kleine Vehn, J., Vanneste, S., Kania, U., Grunewald, W., … Friml, J. (2013). SCF^TIR1 AFB-auxin signalling regulates PIN vacuolar trafficking and auxin fluxes during root gravitropism. EMBO Journal. Wiley-Blackwell. https://doi.org/10.1038/emboj.2012.310","ama":"Baster P, Robert S, Kleine Vehn J, et al. SCF^TIR1 AFB-auxin signalling regulates PIN vacuolar trafficking and auxin fluxes during root gravitropism. EMBO Journal. 2013;32(2):260-274. doi:10.1038/emboj.2012.310","short":"P. Baster, S. Robert, J. Kleine Vehn, S. Vanneste, U. Kania, W. Grunewald, B. De Rybel, T. Beeckman, J. Friml, EMBO Journal 32 (2013) 260–274.","ieee":"P. Baster et al., “SCF^TIR1 AFB-auxin signalling regulates PIN vacuolar trafficking and auxin fluxes during root gravitropism,” EMBO Journal, vol. 32, no. 2. Wiley-Blackwell, pp. 260–274, 2013.","chicago":"Baster, Pawel, Stéphanie Robert, Jürgen Kleine Vehn, Steffen Vanneste, Urszula Kania, Wim Grunewald, Bert De Rybel, Tom Beeckman, and Jiří Friml. “SCF^TIR1 AFB-Auxin Signalling Regulates PIN Vacuolar Trafficking and Auxin Fluxes during Root Gravitropism.” EMBO Journal. Wiley-Blackwell, 2013. https://doi.org/10.1038/emboj.2012.310.","ista":"Baster P, Robert S, Kleine Vehn J, Vanneste S, Kania U, Grunewald W, De Rybel B, Beeckman T, Friml J. 2013. SCF^TIR1 AFB-auxin signalling regulates PIN vacuolar trafficking and auxin fluxes during root gravitropism. EMBO Journal. 32(2), 260–274."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","external_id":{"pmid":["23211744"]},"publist_id":"3818","author":[{"full_name":"Baster, Pawel","last_name":"Baster","first_name":"Pawel","id":"3028BD74-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Stéphanie","full_name":"Robert, Stéphanie","last_name":"Robert"},{"last_name":"Kleine Vehn","full_name":"Kleine Vehn, Jürgen","first_name":"Jürgen"},{"full_name":"Vanneste, Steffen","last_name":"Vanneste","first_name":"Steffen"},{"first_name":"Urszula","id":"4AE5C486-F248-11E8-B48F-1D18A9856A87","full_name":"Kania, Urszula","last_name":"Kania"},{"last_name":"Grunewald","full_name":"Grunewald, Wim","first_name":"Wim"},{"last_name":"De Rybel","full_name":"De Rybel, Bert","first_name":"Bert"},{"full_name":"Beeckman, Tom","last_name":"Beeckman","first_name":"Tom"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","last_name":"Friml"}],"title":"SCF^TIR1 AFB-auxin signalling regulates PIN vacuolar trafficking and auxin fluxes during root gravitropism","year":"2013","publication":"EMBO Journal","day":"23","page":"260 - 274","date_created":"2018-12-11T12:00:20Z","date_published":"2013-01-23T00:00:00Z","doi":"10.1038/emboj.2012.310","oa":1,"quality_controlled":"1","publisher":"Wiley-Blackwell","date_updated":"2021-01-12T07:00:41Z","department":[{"_id":"JiFr"}],"_id":"2919","type":"journal_article","status":"public","publication_status":"published","language":[{"iso":"eng"}],"volume":32,"issue":"2","abstract":[{"lang":"eng","text":"The distribution of the phytohormone auxin regulates many aspects of plant development including growth response to gravity. Gravitropic root curvature involves coordinated and asymmetric cell elongation between the lower and upper side of the root, mediated by differential cellular auxin levels. The asymmetry in the auxin distribution is established and maintained by a spatio-temporal regulation of the PIN-FORMED (PIN) auxin transporter activity. We provide novel insights into the complex regulation of PIN abundance and activity during root gravitropism. We show that PIN2 turnover is differentially regulated on the upper and lower side of gravistimulated roots by distinct but partially overlapping auxin feedback mechanisms. In addition to regulating transcription and clathrin-mediated internalization, auxin also controls PIN abundance at the plasma membrane by promoting their vacuolar targeting and degradation. This effect of elevated auxin levels requires the activity of SKP-Cullin-F-box TIR1/AFB (SCF TIR1/AFB)-dependent pathway. Importantly, also suboptimal auxin levels mediate PIN degradation utilizing the same signalling pathway. These feedback mechanisms are functionally important during gravitropic response and ensure fine-tuning of auxin fluxes for maintaining as well as terminating asymmetric growth."}],"pmid":1,"oa_version":"Submitted Version","main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3553380/"}],"scopus_import":1,"intvolume":" 32","month":"01"},{"external_id":{"pmid":["23975899"]},"author":[{"first_name":"Simone","full_name":"Di Rubbo, Simone","last_name":"Di Rubbo"},{"first_name":"Niloufer","last_name":"Irani","full_name":"Irani, Niloufer"},{"full_name":"Kim, Soo","last_name":"Kim","first_name":"Soo"},{"full_name":"Xu, Zheng","last_name":"Xu","first_name":"Zheng"},{"last_name":"Gadeyne","full_name":"Gadeyne, Astrid","first_name":"Astrid"},{"first_name":"Wim","last_name":"Dejonghe","full_name":"Dejonghe, Wim"},{"first_name":"Isabelle","full_name":"Vanhoutte, Isabelle","last_name":"Vanhoutte"},{"first_name":"Geert","last_name":"Persiau","full_name":"Persiau, Geert"},{"first_name":"Dominique","last_name":"Eeckhout","full_name":"Eeckhout, Dominique"},{"last_name":"Simon","orcid":"0000-0002-1998-6741","full_name":"Simon, Sibu","first_name":"Sibu","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Song, Kyungyoung","last_name":"Song","first_name":"Kyungyoung"},{"first_name":"Jürgen","full_name":"Kleine Vehn, Jürgen","last_name":"Kleine Vehn"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí","last_name":"Friml","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596"},{"full_name":"De Jaeger, Geert","last_name":"De Jaeger","first_name":"Geert"},{"full_name":"Van Damme, Daniël","last_name":"Van Damme","first_name":"Daniël"},{"last_name":"Hwang","full_name":"Hwang, Inhwan","first_name":"Inhwan"},{"first_name":"Eugenia","last_name":"Russinova","full_name":"Russinova, Eugenia"}],"publist_id":"7311","title":"The clathrin adaptor complex AP-2 mediates endocytosis of brassinosteroid INSENSITIVE1 in arabidopsis","citation":{"mla":"Di Rubbo, Simone, et al. “The Clathrin Adaptor Complex AP-2 Mediates Endocytosis of Brassinosteroid INSENSITIVE1 in Arabidopsis.” Plant Cell, vol. 25, no. 8, American Society of Plant Biologists, 2013, pp. 2986–97, doi:10.1105/tpc.113.114058.","ama":"Di Rubbo S, Irani N, Kim S, et al. The clathrin adaptor complex AP-2 mediates endocytosis of brassinosteroid INSENSITIVE1 in arabidopsis. Plant Cell. 2013;25(8):2986-2997. doi:10.1105/tpc.113.114058","apa":"Di Rubbo, S., Irani, N., Kim, S., Xu, Z., Gadeyne, A., Dejonghe, W., … Russinova, E. (2013). The clathrin adaptor complex AP-2 mediates endocytosis of brassinosteroid INSENSITIVE1 in arabidopsis. Plant Cell. American Society of Plant Biologists. https://doi.org/10.1105/tpc.113.114058","ieee":"S. Di Rubbo et al., “The clathrin adaptor complex AP-2 mediates endocytosis of brassinosteroid INSENSITIVE1 in arabidopsis,” Plant Cell, vol. 25, no. 8. American Society of Plant Biologists, pp. 2986–2997, 2013.","short":"S. Di Rubbo, N. Irani, S. Kim, Z. Xu, A. Gadeyne, W. Dejonghe, I. Vanhoutte, G. Persiau, D. Eeckhout, S. Simon, K. Song, J. Kleine Vehn, J. Friml, G. De Jaeger, D. Van Damme, I. Hwang, E. Russinova, Plant Cell 25 (2013) 2986–2997.","chicago":"Di Rubbo, Simone, Niloufer Irani, Soo Kim, Zheng Xu, Astrid Gadeyne, Wim Dejonghe, Isabelle Vanhoutte, et al. “The Clathrin Adaptor Complex AP-2 Mediates Endocytosis of Brassinosteroid INSENSITIVE1 in Arabidopsis.” Plant Cell. American Society of Plant Biologists, 2013. https://doi.org/10.1105/tpc.113.114058.","ista":"Di Rubbo S, Irani N, Kim S, Xu Z, Gadeyne A, Dejonghe W, Vanhoutte I, Persiau G, Eeckhout D, Simon S, Song K, Kleine Vehn J, Friml J, De Jaeger G, Van Damme D, Hwang I, Russinova E. 2013. The clathrin adaptor complex AP-2 mediates endocytosis of brassinosteroid INSENSITIVE1 in arabidopsis. Plant Cell. 25(8), 2986–2997."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","page":"2986 - 2997","date_created":"2018-12-11T11:46:52Z","doi":"10.1105/tpc.113.114058","date_published":"2013-08-01T00:00:00Z","year":"2013","publication":"Plant Cell","day":"01","oa":1,"publisher":"American Society of Plant Biologists","quality_controlled":"1","department":[{"_id":"JiFr"}],"date_updated":"2021-01-12T08:01:13Z","type":"journal_article","status":"public","_id":"509","volume":25,"issue":"8","publication_status":"published","language":[{"iso":"eng"}],"main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3784593/","open_access":"1"}],"scopus_import":1,"intvolume":" 25","month":"08","abstract":[{"text":"Clathrin-mediated endocytosis (CME) regulates many aspects of plant development, including hormone signaling and responses to environmental stresses. Despite the importance of this process, the machinery that regulates CME in plants is largely unknown. In mammals, the heterotetrameric ADAPTOR PROTEIN COMPLEX-2 (AP-2) is required for the formation of clathrin-coated vesicles at the plasma membrane (PM). Although the existence of AP-2 has been predicted in Arabidopsis thaliana, the biochemistry and functionality of the complex is still uncharacterized. Here, we identified all the subunits of the Arabidopsis AP-2 by tandem affinity purification and found that one of the large AP-2 subunits, AP2A1, localized at the PM and interacted with clathrin. Furthermore, endocytosis of the leucine-rich repeat receptor kinase, BRASSINOSTEROID INSENSITIVE1 (BRI1), was shown to depend on AP-2. Knockdown of the two Arabidopsis AP2A genes or overexpression of a dominant-negative version of the medium AP-2 subunit, AP2M, impaired BRI1 endocytosis and enhanced the brassinosteroid signaling. Our data reveal that the CME machinery in Arabidopsis is evolutionarily conserved and that AP-2 functions in receptormediated endocytosis. ","lang":"eng"}],"oa_version":"Submitted Version","pmid":1},{"language":[{"iso":"eng"}],"publication_status":"published","volume":25,"issue":"8","pmid":1,"oa_version":"Submitted Version","abstract":[{"text":"Fertilization in flowering plants requires the temporal and spatial coordination of many developmental processes, including pollen production, anther dehiscence, ovule production, and pollen tube elongation. However, it remains elusive as to how this coordination occurs during reproduction. Here, we present evidence that endocytosis, involving heterotetrameric adaptor protein complex 2 (AP-2), plays a crucial role in fertilization. An Arabidopsis thaliana mutant ap2m displays multiple defects in pollen production and viability, as well as elongation of staminal filaments and pollen tubes, all of which are pivotal processes needed for fertilization. Of these abnormalities, the defects in elongation of staminal filaments and pollen tubes were partially rescued by exogenous auxin. Moreover, DR5rev:GFP (for green fluorescent protein) expression was greatly reduced in filaments and anthers in ap2m mutant plants. At the cellular level, ap2m mutants displayed defects in both endocytosis of N-(3-triethylammonium-propyl)-4- (4-diethylaminophenylhexatrienyl) pyridinium dibromide, a lypophilic dye used as an endocytosis marker, and polar localization of auxin-efflux carrier PIN FORMED2 (PIN2) in the stamen filaments. Moreover, these defects were phenocopied by treatment with Tyrphostin A23, an inhibitor of endocytosis. Based on these results, we propose that AP-2-dependent endocytosis plays a crucial role in coordinating the multiple developmental aspects of male reproductive organs by modulating cellular auxin level through the regulation of the amount and polarity of PINs.","lang":"eng"}],"month":"08","intvolume":" 25","scopus_import":1,"main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3784592/"}],"date_updated":"2021-01-12T08:01:12Z","department":[{"_id":"JiFr"}],"_id":"507","status":"public","type":"journal_article","day":"01","publication":"Plant Cell","year":"2013","doi":"10.1105/tpc.113.114264","date_published":"2013-08-01T00:00:00Z","date_created":"2018-12-11T11:46:52Z","page":"2970 - 2985","publisher":"American Society of Plant Biologists","quality_controlled":"1","oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"mla":"Kim, Soo, et al. “Adaptor Protein Complex 2-Mediated Endocytosis Is Crucial for Male Reproductive Organ Development in Arabidopsis.” Plant Cell, vol. 25, no. 8, American Society of Plant Biologists, 2013, pp. 2970–85, doi:10.1105/tpc.113.114264.","short":"S. Kim, Z. Xu, K. Song, D. Kim, H. Kang, I. Reichardt, E. Sohn, J. Friml, G. Juergens, I. Hwang, Plant Cell 25 (2013) 2970–2985.","ieee":"S. Kim et al., “Adaptor protein complex 2-mediated endocytosis is crucial for male reproductive organ development in arabidopsis,” Plant Cell, vol. 25, no. 8. American Society of Plant Biologists, pp. 2970–2985, 2013.","apa":"Kim, S., Xu, Z., Song, K., Kim, D., Kang, H., Reichardt, I., … Hwang, I. (2013). Adaptor protein complex 2-mediated endocytosis is crucial for male reproductive organ development in arabidopsis. Plant Cell. American Society of Plant Biologists. https://doi.org/10.1105/tpc.113.114264","ama":"Kim S, Xu Z, Song K, et al. Adaptor protein complex 2-mediated endocytosis is crucial for male reproductive organ development in arabidopsis. Plant Cell. 2013;25(8):2970-2985. doi:10.1105/tpc.113.114264","chicago":"Kim, Soo, Zheng Xu, Kyungyoung Song, Dae Kim, Hyangju Kang, Ilka Reichardt, Eun Sohn, Jiří Friml, Gerd Juergens, and Inhwan Hwang. “Adaptor Protein Complex 2-Mediated Endocytosis Is Crucial for Male Reproductive Organ Development in Arabidopsis.” Plant Cell. American Society of Plant Biologists, 2013. https://doi.org/10.1105/tpc.113.114264.","ista":"Kim S, Xu Z, Song K, Kim D, Kang H, Reichardt I, Sohn E, Friml J, Juergens G, Hwang I. 2013. Adaptor protein complex 2-mediated endocytosis is crucial for male reproductive organ development in arabidopsis. Plant Cell. 25(8), 2970–2985."},"title":"Adaptor protein complex 2-mediated endocytosis is crucial for male reproductive organ development in arabidopsis","publist_id":"7312","author":[{"first_name":"Soo","full_name":"Kim, Soo","last_name":"Kim"},{"last_name":"Xu","full_name":"Xu, Zheng","first_name":"Zheng"},{"last_name":"Song","full_name":"Song, Kyungyoung","first_name":"Kyungyoung"},{"last_name":"Kim","full_name":"Kim, Dae","first_name":"Dae"},{"last_name":"Kang","full_name":"Kang, Hyangju","first_name":"Hyangju"},{"first_name":"Ilka","last_name":"Reichardt","full_name":"Reichardt, Ilka"},{"first_name":"Eun","full_name":"Sohn, Eun","last_name":"Sohn"},{"full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","last_name":"Friml","first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Gerd","last_name":"Juergens","full_name":"Juergens, Gerd"},{"full_name":"Hwang, Inhwan","last_name":"Hwang","first_name":"Inhwan"}],"external_id":{"pmid":["23975898"]}},{"publisher":"American Society of Plant Biologists","quality_controlled":"1","oa":1,"day":"01","publication":"Plant Cell","year":"2013","date_published":"2013-10-01T00:00:00Z","doi":"10.1105/tpc.113.114421","date_created":"2018-12-11T11:46:53Z","page":"3858 - 3870","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"ista":"Pěnčík A, Simonovik B, Petersson S, Henyková E, Simon S, Greenham K, Zhang Y, Kowalczyk M, Estelle M, Zažímalová E, Novák O, Sandberg G, Ljung K. 2013. Regulation of auxin homeostasis and gradients in Arabidopsis roots through the formation of the indole-3-acetic acid catabolite 2-oxindole-3-acetic acid. Plant Cell. 25(10), 3858–3870.","chicago":"Pěnčík, Aleš, Biljana Simonovik, Sara Petersson, Eva Henyková, Sibu Simon, Kathleen Greenham, Yi Zhang, et al. “Regulation of Auxin Homeostasis and Gradients in Arabidopsis Roots through the Formation of the Indole-3-Acetic Acid Catabolite 2-Oxindole-3-Acetic Acid.” Plant Cell. American Society of Plant Biologists, 2013. https://doi.org/10.1105/tpc.113.114421.","ieee":"A. Pěnčík et al., “Regulation of auxin homeostasis and gradients in Arabidopsis roots through the formation of the indole-3-acetic acid catabolite 2-oxindole-3-acetic acid,” Plant Cell, vol. 25, no. 10. American Society of Plant Biologists, pp. 3858–3870, 2013.","short":"A. Pěnčík, B. Simonovik, S. Petersson, E. Henyková, S. Simon, K. Greenham, Y. Zhang, M. Kowalczyk, M. Estelle, E. Zažímalová, O. Novák, G. Sandberg, K. Ljung, Plant Cell 25 (2013) 3858–3870.","apa":"Pěnčík, A., Simonovik, B., Petersson, S., Henyková, E., Simon, S., Greenham, K., … Ljung, K. (2013). Regulation of auxin homeostasis and gradients in Arabidopsis roots through the formation of the indole-3-acetic acid catabolite 2-oxindole-3-acetic acid. Plant Cell. American Society of Plant Biologists. https://doi.org/10.1105/tpc.113.114421","ama":"Pěnčík A, Simonovik B, Petersson S, et al. Regulation of auxin homeostasis and gradients in Arabidopsis roots through the formation of the indole-3-acetic acid catabolite 2-oxindole-3-acetic acid. Plant Cell. 2013;25(10):3858-3870. doi:10.1105/tpc.113.114421","mla":"Pěnčík, Aleš, et al. “Regulation of Auxin Homeostasis and Gradients in Arabidopsis Roots through the Formation of the Indole-3-Acetic Acid Catabolite 2-Oxindole-3-Acetic Acid.” Plant Cell, vol. 25, no. 10, American Society of Plant Biologists, 2013, pp. 3858–70, doi:10.1105/tpc.113.114421."},"title":"Regulation of auxin homeostasis and gradients in Arabidopsis roots through the formation of the indole-3-acetic acid catabolite 2-oxindole-3-acetic acid","publist_id":"7309","author":[{"first_name":"Aleš","last_name":"Pěnčík","full_name":"Pěnčík, Aleš"},{"first_name":"Biljana","full_name":"Simonovik, Biljana","last_name":"Simonovik"},{"first_name":"Sara","last_name":"Petersson","full_name":"Petersson, Sara"},{"last_name":"Henyková","full_name":"Henyková, Eva","first_name":"Eva"},{"last_name":"Simon","orcid":"0000-0002-1998-6741","full_name":"Simon, Sibu","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87","first_name":"Sibu"},{"first_name":"Kathleen","last_name":"Greenham","full_name":"Greenham, Kathleen"},{"full_name":"Zhang, Yi","last_name":"Zhang","first_name":"Yi"},{"full_name":"Kowalczyk, Mariusz","last_name":"Kowalczyk","first_name":"Mariusz"},{"first_name":"Mark","full_name":"Estelle, Mark","last_name":"Estelle"},{"full_name":"Zažímalová, Eva","last_name":"Zažímalová","first_name":"Eva"},{"first_name":"Ondřej","full_name":"Novák, Ondřej","last_name":"Novák"},{"first_name":"Göran","full_name":"Sandberg, Göran","last_name":"Sandberg"},{"last_name":"Ljung","full_name":"Ljung, Karin","first_name":"Karin"}],"external_id":{"pmid":["24163311"]},"oa_version":"Published Version","pmid":1,"abstract":[{"lang":"eng","text":"The native auxin, indole-3-acetic acid (IAA), is a major regulator of plant growth and development. Its nonuniform distribution between cells and tissues underlies the spatiotemporal coordination of many developmental events and responses to environmental stimuli. The regulation of auxin gradients and the formation of auxin maxima/minima most likely involve the regulation of both metabolic and transport processes. In this article, we have demonstrated that 2-oxindole-3-acetic acid (oxIAA) is a major primary IAA catabolite formed in Arabidopsis thaliana root tissues. OxIAA had little biological activity and was formed rapidly and irreversibly in response to increases in auxin levels. We further showed that there is cell type-specific regulation of oxIAA levels in the Arabidopsis root apex. We propose that oxIAA is an important element in the regulation of output from auxin gradients and, therefore, in the regulation of auxin homeostasis and response mechanisms."}],"month":"10","intvolume":" 25","scopus_import":1,"main_file_link":[{"open_access":"1","url":"www.doi.org/10.1105/tpc.113.114421"}],"language":[{"iso":"eng"}],"publication_status":"published","issue":"10","volume":25,"_id":"511","status":"public","type":"journal_article","date_updated":"2021-01-12T08:01:15Z","department":[{"_id":"JiFr"}]},{"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","citation":{"ieee":"B. Bargmann et al., “A map of cell type‐specific auxin responses,” Molecular Systems Biology, vol. 9, no. 1. Nature Publishing Group, 2013.","short":"B. Bargmann, S. Vanneste, G. Krouk, T. Nawy, I. Efroni, E. Shani, G. Choe, J. Friml, D. Bergmann, M. Estelle, K. Birnbaum, Molecular Systems Biology 9 (2013).","ama":"Bargmann B, Vanneste S, Krouk G, et al. A map of cell type‐specific auxin responses. Molecular Systems Biology. 2013;9(1). doi:10.1038/msb.2013.40","apa":"Bargmann, B., Vanneste, S., Krouk, G., Nawy, T., Efroni, I., Shani, E., … Birnbaum, K. (2013). A map of cell type‐specific auxin responses. Molecular Systems Biology. Nature Publishing Group. https://doi.org/10.1038/msb.2013.40","mla":"Bargmann, Bastiaan, et al. “A Map of Cell Type‐specific Auxin Responses.” Molecular Systems Biology, vol. 9, no. 1, 688, Nature Publishing Group, 2013, doi:10.1038/msb.2013.40.","ista":"Bargmann B, Vanneste S, Krouk G, Nawy T, Efroni I, Shani E, Choe G, Friml J, Bergmann D, Estelle M, Birnbaum K. 2013. A map of cell type‐specific auxin responses. Molecular Systems Biology. 9(1), 688.","chicago":"Bargmann, Bastiaan, Steffen Vanneste, Gabriel Krouk, Tal Nawy, Idan Efroni, Eilon Shani, Goh Choe, et al. “A Map of Cell Type‐specific Auxin Responses.” Molecular Systems Biology. Nature Publishing Group, 2013. https://doi.org/10.1038/msb.2013.40."},"title":"A map of cell type‐specific auxin responses","article_processing_charge":"No","author":[{"first_name":"Bastiaan","full_name":"Bargmann, Bastiaan","last_name":"Bargmann"},{"last_name":"Vanneste","full_name":"Vanneste, Steffen","first_name":"Steffen"},{"last_name":"Krouk","full_name":"Krouk, Gabriel","first_name":"Gabriel"},{"first_name":"Tal","full_name":"Nawy, Tal","last_name":"Nawy"},{"first_name":"Idan","full_name":"Efroni, Idan","last_name":"Efroni"},{"last_name":"Shani","full_name":"Shani, Eilon","first_name":"Eilon"},{"first_name":"Goh","full_name":"Choe, Goh","last_name":"Choe"},{"first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596"},{"last_name":"Bergmann","full_name":"Bergmann, Dominique","first_name":"Dominique"},{"first_name":"Mark","last_name":"Estelle","full_name":"Estelle, Mark"},{"full_name":"Birnbaum, Kenneth","last_name":"Birnbaum","first_name":"Kenneth"}],"publist_id":"7303","article_number":"688","publication":"Molecular Systems Biology","day":"10","year":"2013","has_accepted_license":"1","date_created":"2018-12-11T11:46:55Z","doi":"10.1038/msb.2013.40","date_published":"2013-09-10T00:00:00Z","oa":1,"publisher":"Nature Publishing Group","quality_controlled":"1","ddc":["581"],"date_updated":"2021-01-12T08:01:17Z","file_date_updated":"2020-07-14T12:46:36Z","department":[{"_id":"JiFr"}],"_id":"516","pubrep_id":"936","status":"public","tmp":{"name":"Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)","image":"/images/cc_by_nc_sa.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode","short":"CC BY-NC-SA (4.0)"},"type":"journal_article","language":[{"iso":"eng"}],"file":[{"file_id":"4644","checksum":"9c4fbe793af4bb22b3fe50cc677a39bf","relation":"main_file","access_level":"open_access","content_type":"application/pdf","file_name":"IST-2018-936-v1+1_2008_Barton_A_map.pdf","date_created":"2018-12-12T10:07:46Z","creator":"system","file_size":3257692,"date_updated":"2020-07-14T12:46:36Z"}],"publication_status":"published","license":"https://creativecommons.org/licenses/by-nc-sa/4.0/","issue":"1","volume":9,"oa_version":"Published Version","abstract":[{"lang":"eng","text":"In plants, changes in local auxin concentrations can trigger a range of developmental processes as distinct tissues respond differently to the same auxin stimulus. However, little is known about how auxin is interpreted by individual cell types. We performed a transcriptomic analysis of responses to auxin within four distinct tissues of the Arabidopsis thaliana root and demonstrate that different cell types show competence for discrete responses. The majority of auxin‐responsive genes displayed a spatial bias in their induction or repression. The novel data set was used to examine how auxin influences tissue‐specific transcriptional regulation of cell‐identity markers. Additionally, the data were used in combination with spatial expression maps of the root to plot a transcriptomic auxin‐response gradient across the apical and basal meristem. The readout revealed a strong correlation for thousands of genes between the relative response to auxin and expression along the longitudinal axis of the root. This data set and comparative analysis provide a transcriptome‐level spatial breakdown of the response to auxin within an organ where this hormone mediates many aspects of development."}],"intvolume":" 9","month":"09","scopus_import":1},{"_id":"528","status":"public","project":[{"name":"Polarity and subcellular dynamics in plants","grant_number":"282300","call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425"}],"type":"journal_article","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"chicago":"Robert, Hélène, Peter Grones, Anna Stepanova, Linda Robles, Annemarie Lokerse, Jose Alonso, Dolf Weijers, and Jiří Friml. “Local Auxin Sources Orient the Apical Basal Axis in Arabidopsis Embryos.” Current Biology. Cell Press, 2013. https://doi.org/10.1016/j.cub.2013.09.039.","ista":"Robert H, Grones P, Stepanova A, Robles L, Lokerse A, Alonso J, Weijers D, Friml J. 2013. Local auxin sources orient the apical basal axis in arabidopsis embryos. Current Biology. 23(24), 2506–2512.","mla":"Robert, Hélène, et al. “Local Auxin Sources Orient the Apical Basal Axis in Arabidopsis Embryos.” Current Biology, vol. 23, no. 24, Cell Press, 2013, pp. 2506–12, doi:10.1016/j.cub.2013.09.039.","apa":"Robert, H., Grones, P., Stepanova, A., Robles, L., Lokerse, A., Alonso, J., … Friml, J. (2013). Local auxin sources orient the apical basal axis in arabidopsis embryos. Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2013.09.039","ama":"Robert H, Grones P, Stepanova A, et al. Local auxin sources orient the apical basal axis in arabidopsis embryos. Current Biology. 2013;23(24):2506-2512. doi:10.1016/j.cub.2013.09.039","ieee":"H. Robert et al., “Local auxin sources orient the apical basal axis in arabidopsis embryos,” Current Biology, vol. 23, no. 24. Cell Press, pp. 2506–2512, 2013.","short":"H. Robert, P. Grones, A. Stepanova, L. Robles, A. Lokerse, J. Alonso, D. Weijers, J. Friml, Current Biology 23 (2013) 2506–2512."},"date_updated":"2021-01-12T08:01:25Z","department":[{"_id":"JiFr"}],"title":"Local auxin sources orient the apical basal axis in arabidopsis embryos","publist_id":"7291","author":[{"full_name":"Robert, Hélène","last_name":"Robert","first_name":"Hélène"},{"id":"399876EC-F248-11E8-B48F-1D18A9856A87","first_name":"Peter","full_name":"Grones, Peter","last_name":"Grones"},{"full_name":"Stepanova, Anna","last_name":"Stepanova","first_name":"Anna"},{"last_name":"Robles","full_name":"Robles, Linda","first_name":"Linda"},{"first_name":"Annemarie","full_name":"Lokerse, Annemarie","last_name":"Lokerse"},{"full_name":"Alonso, Jose","last_name":"Alonso","first_name":"Jose"},{"full_name":"Weijers, Dolf","last_name":"Weijers","first_name":"Dolf"},{"first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","last_name":"Friml"}],"oa_version":"None","abstract":[{"lang":"eng","text":"Establishment of the embryonic axis foreshadows the main body axis of adults both in plants and in animals, but underlying mechanisms are considered distinct. Plants utilize directional, cell-to-cell transport of the growth hormone auxin [1, 2] to generate an asymmetric auxin response that specifies the embryonic apical-basal axis [3-6]. The auxin flow directionality depends on the polarized subcellular localization of PIN-FORMED (PIN) auxin transporters [7, 8]. It remains unknown which mechanisms and spatial cues guide cell polarization and axis orientation in early embryos. Herein, we provide conceptually novel insights into the formation of embryonic axis in Arabidopsis by identifying a crucial role of localized tryptophan-dependent auxin biosynthesis [9-12]. Local auxin production at the base of young embryos and the accompanying PIN7-mediated auxin flow toward the proembryo are required for the apical auxin response maximum and the specification of apical embryonic structures. Later in embryogenesis, the precisely timed onset of localized apical auxin biosynthesis mediates PIN1 polarization, basal auxin response maximum, and specification of the root pole. Thus, the tight spatiotemporal control of distinct local auxin sources provides a necessary, non-cell-autonomous trigger for the coordinated cell polarization and subsequent apical-basal axis orientation during embryogenesis and, presumably, also for other polarization events during postembryonic plant life [13, 14]."}],"month":"12","intvolume":" 23","scopus_import":1,"publisher":"Cell Press","quality_controlled":"1","day":"16","publication":"Current Biology","language":[{"iso":"eng"}],"publication_status":"published","year":"2013","date_published":"2013-12-16T00:00:00Z","issue":"24","volume":23,"doi":"10.1016/j.cub.2013.09.039","ec_funded":1,"date_created":"2018-12-11T11:46:59Z","page":"2506 - 2512"},{"title":"Modeling framework for the establishment of the apical-basal embryonic axis in plants","department":[{"_id":"EvBe"},{"_id":"JiFr"}],"author":[{"orcid":"0000-0001-7263-0560","full_name":"Wabnik, Krzysztof T","last_name":"Wabnik","id":"4DE369A4-F248-11E8-B48F-1D18A9856A87","first_name":"Krzysztof T"},{"full_name":"Robert, Hélène","last_name":"Robert","first_name":"Hélène"},{"last_name":"Smith","full_name":"Smith, Richard","first_name":"Richard"},{"first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","last_name":"Friml"}],"publist_id":"7292","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"mla":"Wabnik, Krzysztof T., et al. “Modeling Framework for the Establishment of the Apical-Basal Embryonic Axis in Plants.” Current Biology, vol. 23, no. 24, Cell Press, 2013, pp. 2513–18, doi:10.1016/j.cub.2013.10.038.","short":"K.T. Wabnik, H. Robert, R. Smith, J. Friml, Current Biology 23 (2013) 2513–2518.","ieee":"K. T. Wabnik, H. Robert, R. Smith, and J. Friml, “Modeling framework for the establishment of the apical-basal embryonic axis in plants,” Current Biology, vol. 23, no. 24. Cell Press, pp. 2513–2518, 2013.","ama":"Wabnik KT, Robert H, Smith R, Friml J. Modeling framework for the establishment of the apical-basal embryonic axis in plants. Current Biology. 2013;23(24):2513-2518. doi:10.1016/j.cub.2013.10.038","apa":"Wabnik, K. T., Robert, H., Smith, R., & Friml, J. (2013). Modeling framework for the establishment of the apical-basal embryonic axis in plants. Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2013.10.038","chicago":"Wabnik, Krzysztof T, Hélène Robert, Richard Smith, and Jiří Friml. “Modeling Framework for the Establishment of the Apical-Basal Embryonic Axis in Plants.” Current Biology. Cell Press, 2013. https://doi.org/10.1016/j.cub.2013.10.038.","ista":"Wabnik KT, Robert H, Smith R, Friml J. 2013. Modeling framework for the establishment of the apical-basal embryonic axis in plants. Current Biology. 23(24), 2513–2518."},"date_updated":"2021-01-12T08:01:24Z","project":[{"name":"Polarity and subcellular dynamics in plants","grant_number":"282300","call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425"}],"status":"public","type":"journal_article","_id":"527","date_created":"2018-12-11T11:46:58Z","ec_funded":1,"issue":"24","volume":23,"doi":"10.1016/j.cub.2013.10.038","date_published":"2013-12-16T00:00:00Z","page":"2513 - 2518","publication":"Current Biology","language":[{"iso":"eng"}],"day":"16","year":"2013","publication_status":"published","intvolume":" 23","month":"12","publisher":"Cell Press","scopus_import":1,"quality_controlled":"1","oa_version":"None","abstract":[{"text":"The apical-basal axis of the early plant embryo determines the body plan of the adult organism. To establish a polarized embryonic axis, plants evolved a unique mechanism that involves directional, cell-to-cell transport of the growth regulator auxin. Auxin transport relies on PIN auxin transporters [1], whose polar subcellular localization determines the flow directionality. PIN-mediated auxin transport mediates the spatial and temporal activity of the auxin response machinery [2-7] that contributes to embryo patterning processes, including establishment of the apical (shoot) and basal (root) embryo poles [8]. However, little is known of upstream mechanisms guiding the (re)polarization of auxin fluxes during embryogenesis [9]. Here, we developed a model of plant embryogenesis that correctly generates emergent cell polarities and auxin-mediated sequential initiation of apical-basal axis of plant embryo. The model relies on two precisely localized auxin sources and a feedback between auxin and the polar, subcellular PIN transporter localization. Simulations reproduced PIN polarity and auxin distribution, as well as previously unknown polarization events during early embryogenesis. The spectrum of validated model predictions suggests that our model corresponds to a minimal mechanistic framework for initiation and orientation of the apical-basal axis to guide both embryonic and postembryonic plant development.","lang":"eng"}]},{"article_number":"e25688","project":[{"call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425","name":"Polarity and subcellular dynamics in plants","grant_number":"282300"}],"citation":{"mla":"Remy, Estelle, et al. “ZIFL1.1 Transporter Modulates Polar Auxin Transport by Stabilizing Membrane Abundance of Multiple PINs in Arabidopsis Root Tip.” Plant Signaling & Behavior, vol. 8, no. 10, e25688, Taylor & Francis, 2013, doi:10.4161/psb.25688.","apa":"Remy, E., Baster, P., Friml, J., & Duque, P. (2013). ZIFL1.1 transporter modulates polar auxin transport by stabilizing membrane abundance of multiple PINs in Arabidopsis root tip. Plant Signaling & Behavior. Taylor & Francis. https://doi.org/10.4161/psb.25688","ama":"Remy E, Baster P, Friml J, Duque P. ZIFL1.1 transporter modulates polar auxin transport by stabilizing membrane abundance of multiple PINs in Arabidopsis root tip. Plant Signaling & Behavior. 2013;8(10). doi:10.4161/psb.25688","ieee":"E. Remy, P. Baster, J. Friml, and P. Duque, “ZIFL1.1 transporter modulates polar auxin transport by stabilizing membrane abundance of multiple PINs in Arabidopsis root tip,” Plant Signaling & Behavior, vol. 8, no. 10. Taylor & Francis, 2013.","short":"E. Remy, P. Baster, J. Friml, P. Duque, Plant Signaling & Behavior 8 (2013).","chicago":"Remy, Estelle, Pawel Baster, Jiří Friml, and Paula Duque. “ZIFL1.1 Transporter Modulates Polar Auxin Transport by Stabilizing Membrane Abundance of Multiple PINs in Arabidopsis Root Tip.” Plant Signaling & Behavior. Taylor & Francis, 2013. https://doi.org/10.4161/psb.25688.","ista":"Remy E, Baster P, Friml J, Duque P. 2013. ZIFL1.1 transporter modulates polar auxin transport by stabilizing membrane abundance of multiple PINs in Arabidopsis root tip. Plant Signaling & Behavior. 8(10), e25688."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"last_name":"Remy","full_name":"Remy, Estelle","first_name":"Estelle"},{"last_name":"Baster","full_name":"Baster, Pawel","id":"3028BD74-F248-11E8-B48F-1D18A9856A87","first_name":"Pawel"},{"first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596"},{"last_name":"Duque","full_name":"Duque, Paula","first_name":"Paula"}],"publist_id":"4455","external_id":{"pmid":["23857365"]},"article_processing_charge":"No","title":"ZIFL1.1 transporter modulates polar auxin transport by stabilizing membrane abundance of multiple PINs in Arabidopsis root tip","quality_controlled":"1","publisher":"Taylor & Francis","oa":1,"year":"2013","day":"10","publication":"Plant Signaling & Behavior","date_published":"2013-07-10T00:00:00Z","doi":"10.4161/psb.25688","date_created":"2018-12-11T11:57:43Z","_id":"2448","article_type":"original","type":"journal_article","status":"public","date_updated":"2023-10-17T11:15:14Z","department":[{"_id":"JiFr"}],"abstract":[{"lang":"eng","text":"Cell-to-cell directional flow of the phytohormone auxin is primarily established by polar localization of the PIN auxin transporters, a process tightly regulated at multiple levels by auxin itself. We recently reported that, in the context of strong auxin flows, activity of the vacuolar ZIFL1.1 transporter is required for fine-tuning of polar auxin transport rates in the Arabidopsis root. In particular, ZIFL1.1 function protects plasma-membrane stability of the PIN2 carrier in epidermal root tip cells under conditions normally triggering PIN2 degradation. Here, we show that ZIFL1.1 activity at the root tip also promotes PIN1 plasma-membrane abundance in central cylinder cells, thus supporting the notion that ZIFL1.1 acts as a general positive modulator of polar auxin transport in roots."}],"oa_version":"Submitted Version","pmid":1,"scopus_import":"1","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4091088/","open_access":"1"}],"month":"07","intvolume":" 8","publication_status":"published","language":[{"iso":"eng"}],"issue":"10","volume":8,"ec_funded":1}]