[{"doi":"10.1038/s41477-018-0160-7","type":"journal_article","article_processing_charge":"No","page":"365 - 375","publication_status":"published","publication":"Nature Plants","date_published":"2018-05-28T00:00:00Z","citation":{"ieee":"Z. Gao et al., “KIRA1 and ORESARA1 terminate flower receptivity by promoting cell death in the stigma of Arabidopsis,” Nature Plants, vol. 4, no. 6. Nature Publishing Group, pp. 365–375, 2018.","ista":"Gao Z, Daneva A, Salanenka Y, Van Durme M, Huysmans M, Lin Z, De Winter F, Vanneste S, Karimi M, Van De Velde J, Vandepoele K, Van De Walle D, Dewettinck K, Lambrecht B, Nowack M. 2018. KIRA1 and ORESARA1 terminate flower receptivity by promoting cell death in the stigma of Arabidopsis. Nature Plants. 4(6), 365–375.","mla":"Gao, Zhen, et al. “KIRA1 and ORESARA1 Terminate Flower Receptivity by Promoting Cell Death in the Stigma of Arabidopsis.” Nature Plants, vol. 4, no. 6, Nature Publishing Group, 2018, pp. 365–75, doi:10.1038/s41477-018-0160-7.","chicago":"Gao, Zhen, Anna Daneva, Yuliya Salanenka, Matthias Van Durme, Marlies Huysmans, Zongcheng Lin, Freya De Winter, et al. “KIRA1 and ORESARA1 Terminate Flower Receptivity by Promoting Cell Death in the Stigma of Arabidopsis.” Nature Plants. Nature Publishing Group, 2018. https://doi.org/10.1038/s41477-018-0160-7.","apa":"Gao, Z., Daneva, A., Salanenka, Y., Van Durme, M., Huysmans, M., Lin, Z., … Nowack, M. (2018). KIRA1 and ORESARA1 terminate flower receptivity by promoting cell death in the stigma of Arabidopsis. Nature Plants. Nature Publishing Group. https://doi.org/10.1038/s41477-018-0160-7","ama":"Gao Z, Daneva A, Salanenka Y, et al. KIRA1 and ORESARA1 terminate flower receptivity by promoting cell death in the stigma of Arabidopsis. Nature Plants. 2018;4(6):365-375. doi:10.1038/s41477-018-0160-7","short":"Z. Gao, A. Daneva, Y. Salanenka, M. Van Durme, M. Huysmans, Z. Lin, F. De Winter, S. Vanneste, M. Karimi, J. Van De Velde, K. Vandepoele, D. Van De Walle, K. Dewettinck, B. Lambrecht, M. Nowack, Nature Plants 4 (2018) 365–375."},"month":"05","intvolume":" 4","oa_version":"None","scopus_import":"1","day":"28","year":"2018","language":[{"iso":"eng"}],"status":"public","volume":4,"department":[{"_id":"JiFr"}],"author":[{"last_name":"Gao","first_name":"Zhen","full_name":"Gao, Zhen"},{"last_name":"Daneva","full_name":"Daneva, Anna","first_name":"Anna"},{"full_name":"Salanenka, Yuliya","first_name":"Yuliya","last_name":"Salanenka","id":"46DAAE7E-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Van Durme, Matthias","first_name":"Matthias","last_name":"Van Durme"},{"last_name":"Huysmans","full_name":"Huysmans, Marlies","first_name":"Marlies"},{"first_name":"Zongcheng","full_name":"Lin, Zongcheng","last_name":"Lin"},{"last_name":"De Winter","first_name":"Freya","full_name":"De Winter, Freya"},{"last_name":"Vanneste","first_name":"Steffen","full_name":"Vanneste, Steffen"},{"last_name":"Karimi","first_name":"Mansour","full_name":"Karimi, Mansour"},{"last_name":"Van De Velde","full_name":"Van De Velde, Jan","first_name":"Jan"},{"last_name":"Vandepoele","first_name":"Klaas","full_name":"Vandepoele, Klaas"},{"first_name":"Davy","full_name":"Van De Walle, Davy","last_name":"Van De Walle"},{"full_name":"Dewettinck, Koen","first_name":"Koen","last_name":"Dewettinck"},{"last_name":"Lambrecht","first_name":"Bart","full_name":"Lambrecht, Bart"},{"full_name":"Nowack, Moritz","first_name":"Moritz","last_name":"Nowack"}],"abstract":[{"text":"Flowers have a species-specific functional life span that determines the time window in which pollination, fertilization and seed set can occur. The stigma tissue plays a key role in flower receptivity by intercepting pollen and initiating pollen tube growth toward the ovary. In this article, we show that a developmentally controlled cell death programme terminates the functional life span of stigma cells in Arabidopsis. We identified the leaf senescence regulator ORESARA1 (also known as ANAC092) and the previously uncharacterized KIRA1 (also known as ANAC074) as partially redundant transcription factors that modulate stigma longevity by controlling the expression of programmed cell death-associated genes. KIRA1 expression is sufficient to induce cell death and terminate floral receptivity, whereas lack of both KIRA1 and ORESARA1 substantially increases stigma life span. Surprisingly, the extension of stigma longevity is accompanied by only a moderate extension of flower receptivity, suggesting that additional processes participate in the control of the flower's receptive life span.","lang":"eng"}],"issue":"6","publist_id":"7619","publisher":"Nature Publishing Group","date_created":"2018-12-11T11:45:35Z","acknowledgement":"We gratefully acknowledge funding from the Chinese Scholarship Council (CSC; project number 201206910025 to Z.G.), the Fonds Wetenschappelijk Onderzoek (FWO; project number G005112N to A.D.; fellowship number 12I7417N to Z.L.), the Belgian Federal Science Policy Office (BELSPO; to Y.S.), the Agency for Innovation by Science and Technology of Belgium (IWT; fellowship number 121110 to M.V.D.), the Hercules foundation (grant AUGE-09-029 to K.D.), and the ERC StG PROCELLDEATH (project number 639234 to M.K.N.).","external_id":{"isi":["000435571000017"]},"date_updated":"2023-09-13T08:24:17Z","quality_controlled":"1","_id":"280","isi":1,"title":"KIRA1 and ORESARA1 terminate flower receptivity by promoting cell death in the stigma of Arabidopsis","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1"},{"quality_controlled":"1","_id":"158","isi":1,"title":"Maternal auxin supply contributes to early embryo patterning in Arabidopsis","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","abstract":[{"text":"The angiosperm seed is composed of three genetically distinct tissues: the diploid embryo that originates from the fertilized egg cell, the triploid endosperm that is produced from the fertilized central cell, and the maternal sporophytic integuments that develop into the seed coat1. At the onset of embryo development in Arabidopsis thaliana, the zygote divides asymmetrically, producing a small apical embryonic cell and a larger basal cell that connects the embryo to the maternal tissue2. The coordinated and synchronous development of the embryo and the surrounding integuments, and the alignment of their growth axes, suggest communication between maternal tissues and the embryo. In contrast to animals, however, where a network of maternal factors that direct embryo patterning have been identified3,4, only a few maternal mutations have been described to affect embryo development in plants5–7. Early embryo patterning in Arabidopsis requires accumulation of the phytohormone auxin in the apical cell by directed transport from the suspensor8–10. However, the origin of this auxin has remained obscure. Here we investigate the source of auxin for early embryogenesis and provide evidence that the mother plant coordinates seed development by supplying auxin to the early embryo from the integuments of the ovule. We show that auxin response increases in ovules after fertilization, due to upregulated auxin biosynthesis in the integuments, and this maternally produced auxin is required for correct embryo development.","lang":"eng"}],"ec_funded":1,"issue":"8","related_material":{"link":[{"url":"https://ist.ac.at/en/news/plant-mothers-talk-to-their-embryos-via-the-hormone-auxin/","description":"News on IST Homepage","relation":"press_release"}]},"project":[{"call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425","grant_number":"282300","name":"Polarity and subcellular dynamics in plants"}],"oa":1,"publist_id":"7763","publisher":"Nature Publishing Group","date_created":"2018-12-11T11:44:56Z","acknowledgement":"This work was further supported by the Czech Science Foundation GACR (GA13-40637S) to J.F.;","main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pubmed/30013211","open_access":"1"}],"external_id":{"isi":["000443861300011"],"pmid":["30013211"]},"date_updated":"2023-09-13T08:53:28Z","language":[{"iso":"eng"}],"day":"16","year":"2018","status":"public","volume":4,"department":[{"_id":"JiFr"}],"pmid":1,"author":[{"last_name":"Robert","full_name":"Robert, Hélène","first_name":"Hélène"},{"last_name":"Park","full_name":"Park, Chulmin","first_name":"Chulmin"},{"full_name":"Gutièrrez, Carla","first_name":"Carla","last_name":"Gutièrrez"},{"last_name":"Wójcikowska","first_name":"Barbara","full_name":"Wójcikowska, Barbara"},{"full_name":"Pěnčík, Aleš","first_name":"Aleš","last_name":"Pěnčík"},{"last_name":"Novák","first_name":"Ondřej","full_name":"Novák, Ondřej"},{"full_name":"Chen, Junyi","first_name":"Junyi","last_name":"Chen"},{"last_name":"Grunewald","first_name":"Wim","full_name":"Grunewald, Wim"},{"full_name":"Dresselhaus, Thomas","first_name":"Thomas","last_name":"Dresselhaus"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","first_name":"Jirí","orcid":"0000-0002-8302-7596"},{"full_name":"Laux, Thomas","first_name":"Thomas","last_name":"Laux"}],"doi":"10.1038/s41477-018-0204-z","type":"journal_article","article_processing_charge":"No","page":"548 - 553","publication_status":"published","publication":"Nature Plants","date_published":"2018-07-16T00:00:00Z","citation":{"ieee":"H. Robert et al., “Maternal auxin supply contributes to early embryo patterning in Arabidopsis,” Nature Plants, vol. 4, no. 8. Nature Publishing Group, pp. 548–553, 2018.","mla":"Robert, Hélène, et al. “Maternal Auxin Supply Contributes to Early Embryo Patterning in Arabidopsis.” Nature Plants, vol. 4, no. 8, Nature Publishing Group, 2018, pp. 548–53, doi:10.1038/s41477-018-0204-z.","ista":"Robert H, Park C, Gutièrrez C, Wójcikowska B, Pěnčík A, Novák O, Chen J, Grunewald W, Dresselhaus T, Friml J, Laux T. 2018. Maternal auxin supply contributes to early embryo patterning in Arabidopsis. Nature Plants. 4(8), 548–553.","short":"H. Robert, C. Park, C. Gutièrrez, B. Wójcikowska, A. Pěnčík, O. Novák, J. Chen, W. Grunewald, T. Dresselhaus, J. Friml, T. Laux, Nature Plants 4 (2018) 548–553.","apa":"Robert, H., Park, C., Gutièrrez, C., Wójcikowska, B., Pěnčík, A., Novák, O., … Laux, T. (2018). Maternal auxin supply contributes to early embryo patterning in Arabidopsis. Nature Plants. Nature Publishing Group. https://doi.org/10.1038/s41477-018-0204-z","ama":"Robert H, Park C, Gutièrrez C, et al. Maternal auxin supply contributes to early embryo patterning in Arabidopsis. Nature Plants. 2018;4(8):548-553. doi:10.1038/s41477-018-0204-z","chicago":"Robert, Hélène, Chulmin Park, Carla Gutièrrez, Barbara Wójcikowska, Aleš Pěnčík, Ondřej Novák, Junyi Chen, et al. “Maternal Auxin Supply Contributes to Early Embryo Patterning in Arabidopsis.” Nature Plants. Nature Publishing Group, 2018. https://doi.org/10.1038/s41477-018-0204-z."},"intvolume":" 4","month":"07","scopus_import":"1","oa_version":"Submitted Version"},{"author":[{"last_name":"Fan","full_name":"Fan, Ligang","first_name":"Ligang"},{"last_name":"Zhao","first_name":"Lei","full_name":"Zhao, Lei"},{"last_name":"Hu","full_name":"Hu, Wei","first_name":"Wei"},{"last_name":"Li","first_name":"Weina","full_name":"Li, Weina"},{"last_name":"Novák","full_name":"Novák, Ondřej","first_name":"Ondřej"},{"full_name":"Strnad, Miroslav","first_name":"Miroslav","last_name":"Strnad"},{"id":"4542EF9A-F248-11E8-B48F-1D18A9856A87","last_name":"Simon","first_name":"Sibu","full_name":"Simon, Sibu","orcid":"0000-0002-1998-6741"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","first_name":"Jirí","orcid":"0000-0002-8302-7596"},{"first_name":"Jinbo","full_name":"Shen, Jinbo","last_name":"Shen"},{"last_name":"Jiang","full_name":"Jiang, Liwen","first_name":"Liwen"},{"last_name":"Qiu","full_name":"Qiu, Quan","first_name":"Quan"}],"volume":41,"department":[{"_id":"JiFr"}],"pmid":1,"day":"01","language":[{"iso":"eng"}],"year":"2018","status":"public","article_type":"original","has_accepted_license":"1","publication":"Plant, Cell and Environment","date_published":"2018-05-01T00:00:00Z","citation":{"mla":"Fan, Ligang, et al. “NHX Antiporters Regulate the PH of Endoplasmic Reticulum and Auxin-Mediated Development.” Plant, Cell and Environment, vol. 41, Wiley-Blackwell, 2018, pp. 850–64, doi:10.1111/pce.13153.","ista":"Fan L, Zhao L, Hu W, Li W, Novák O, Strnad M, Simon S, Friml J, Shen J, Jiang L, Qiu Q. 2018. NHX antiporters regulate the pH of endoplasmic reticulum and auxin-mediated development. Plant, Cell and Environment. 41, 850–864.","ieee":"L. Fan et al., “NHX antiporters regulate the pH of endoplasmic reticulum and auxin-mediated development,” Plant, Cell and Environment, vol. 41. Wiley-Blackwell, pp. 850–864, 2018.","chicago":"Fan, Ligang, Lei Zhao, Wei Hu, Weina Li, Ondřej Novák, Miroslav Strnad, Sibu Simon, et al. “NHX Antiporters Regulate the PH of Endoplasmic Reticulum and Auxin-Mediated Development.” Plant, Cell and Environment. Wiley-Blackwell, 2018. https://doi.org/10.1111/pce.13153.","apa":"Fan, L., Zhao, L., Hu, W., Li, W., Novák, O., Strnad, M., … Qiu, Q. (2018). NHX antiporters regulate the pH of endoplasmic reticulum and auxin-mediated development. Plant, Cell and Environment. Wiley-Blackwell. https://doi.org/10.1111/pce.13153","ama":"Fan L, Zhao L, Hu W, et al. NHX antiporters regulate the pH of endoplasmic reticulum and auxin-mediated development. Plant, Cell and Environment. 2018;41:850-864. doi:10.1111/pce.13153","short":"L. Fan, L. Zhao, W. Hu, W. Li, O. Novák, M. Strnad, S. Simon, J. Friml, J. Shen, L. Jiang, Q. Qiu, Plant, Cell and Environment 41 (2018) 850–864."},"intvolume":" 41","file_date_updated":"2020-07-14T12:46:32Z","month":"05","oa_version":"Submitted Version","scopus_import":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","short":"CC BY-NC (4.0)","image":"/images/cc_by_nc.png"},"page":"850 - 864","publication_status":"published","doi":"10.1111/pce.13153","type":"journal_article","article_processing_charge":"No","license":"https://creativecommons.org/licenses/by-nc/4.0/","title":"NHX antiporters regulate the pH of endoplasmic reticulum and auxin-mediated development","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","file":[{"access_level":"open_access","content_type":"application/pdf","file_name":"2018_PlantCellEnv_Fan.pdf","date_updated":"2020-07-14T12:46:32Z","relation":"main_file","creator":"dernst","file_id":"7042","date_created":"2019-11-18T16:22:22Z","checksum":"6a20f843565f962cb20281cdf5e40914","file_size":1937976}],"isi":1,"_id":"462","quality_controlled":"1","acknowledgement":"This work was supported by the National Natural Science Foundation of China (31571464, 31371438 and 31070222 to Q.S.Q.), the National Basic Research Program of China (973 project, 2013CB429904 to Q.S.Q.), the Research Fund for the Doctoral Program of Higher Education of China (20130211110001 to Q.S.Q.), the Ministry of Education, Youth and Sports of the Czech Republic (the National Program for Sustainability I, LO1204), and The Czech Science Foundation GAČR (GA13–40637S) to JF. We thank Dr. Tom J. Guilfoyle for DR5::GUS line and Dr. Jia Li for pBIB‐RFP vector and DR5::GFP line. We thank Liping Guan and Yang Zhao for their help with the confocal microscope assay. ","external_id":{"isi":["000426870500012"],"pmid":["29360148"]},"date_updated":"2023-09-13T09:03:18Z","publist_id":"7359","publisher":"Wiley-Blackwell","date_created":"2018-12-11T11:46:36Z","oa":1,"abstract":[{"text":"AtNHX5 and AtNHX6 are endosomal Na+,K+/H+ antiporters that are critical for growth and development in Arabidopsis, but the mechanism behind their action remains unknown. Here, we report that AtNHX5 and AtNHX6, functioning as H+ leak, control auxin homeostasis and auxin-mediated development. We found that nhx5 nhx6 exhibited growth variations of auxin-related defects. We further showed that nhx5 nhx6 was affected in auxin homeostasis. Genetic analysis showed that AtNHX5 and AtNHX6 were required for the function of the ER-localized auxin transporter PIN5. Although AtNHX5 and AtNHX6 were co-localized with PIN5 at ER, they did not interact directly. Instead, the conserved acidic residues in AtNHX5 and AtNHX6, which are essential for exchange activity, were required for PIN5 function. AtNHX5 and AtNHX6 regulated the pH in ER. Overall, AtNHX5 and AtNHX6 may regulate auxin transport across the ER via the pH gradient created by their transport activity. H+-leak pathway provides a fine-tuning mechanism that controls cellular auxin fluxes. ","lang":"eng"}],"ddc":["580"]},{"month":"06","intvolume":" 4","oa_version":"Submitted Version","scopus_import":"1","publication":"Nature Plants","date_published":"2018-06-25T00:00:00Z","citation":{"ama":"Fendrych M, Akhmanova M, Merrin J, et al. Rapid and reversible root growth inhibition by TIR1 auxin signalling. Nature Plants. 2018;4(7):453-459. doi:10.1038/s41477-018-0190-1","apa":"Fendrych, M., Akhmanova, M., Merrin, J., Glanc, M., Hagihara, S., Takahashi, K., … Friml, J. (2018). Rapid and reversible root growth inhibition by TIR1 auxin signalling. Nature Plants. Springer Nature. https://doi.org/10.1038/s41477-018-0190-1","short":"M. Fendrych, M. Akhmanova, J. Merrin, M. Glanc, S. Hagihara, K. Takahashi, N. Uchida, K.U. Torii, J. Friml, Nature Plants 4 (2018) 453–459.","chicago":"Fendrych, Matyas, Maria Akhmanova, Jack Merrin, Matous Glanc, Shinya Hagihara, Koji Takahashi, Naoyuki Uchida, Keiko U Torii, and Jiří Friml. “Rapid and Reversible Root Growth Inhibition by TIR1 Auxin Signalling.” Nature Plants. Springer Nature, 2018. https://doi.org/10.1038/s41477-018-0190-1.","ieee":"M. Fendrych et al., “Rapid and reversible root growth inhibition by TIR1 auxin signalling,” Nature Plants, vol. 4, no. 7. Springer Nature, pp. 453–459, 2018.","mla":"Fendrych, Matyas, et al. “Rapid and Reversible Root Growth Inhibition by TIR1 Auxin Signalling.” Nature Plants, vol. 4, no. 7, Springer Nature, 2018, pp. 453–59, doi:10.1038/s41477-018-0190-1.","ista":"Fendrych M, Akhmanova M, Merrin J, Glanc M, Hagihara S, Takahashi K, Uchida N, Torii KU, Friml J. 2018. Rapid and reversible root growth inhibition by TIR1 auxin signalling. Nature Plants. 4(7), 453–459."},"page":"453 - 459","publication_status":"published","article_processing_charge":"No","doi":"10.1038/s41477-018-0190-1","type":"journal_article","author":[{"first_name":"Matyas","full_name":"Fendrych, Matyas","orcid":"0000-0002-9767-8699","id":"43905548-F248-11E8-B48F-1D18A9856A87","last_name":"Fendrych"},{"last_name":"Akhmanova","id":"3425EC26-F248-11E8-B48F-1D18A9856A87","full_name":"Akhmanova, Maria","first_name":"Maria","orcid":"0000-0003-1522-3162"},{"id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin","orcid":"0000-0001-5145-4609","first_name":"Jack","full_name":"Merrin, Jack"},{"last_name":"Glanc","full_name":"Glanc, Matous","first_name":"Matous"},{"last_name":"Hagihara","first_name":"Shinya","full_name":"Hagihara, Shinya"},{"last_name":"Takahashi","full_name":"Takahashi, Koji","first_name":"Koji"},{"full_name":"Uchida, Naoyuki","first_name":"Naoyuki","last_name":"Uchida"},{"last_name":"Torii","first_name":"Keiko U","full_name":"Torii, Keiko U"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","first_name":"Jirí"}],"department":[{"_id":"JiFr"},{"_id":"DaSi"},{"_id":"NanoFab"}],"pmid":1,"volume":4,"year":"2018","day":"25","language":[{"iso":"eng"}],"status":"public","article_type":"original","external_id":{"isi":["000443221200017"],"pmid":["29942048"]},"date_updated":"2023-09-15T12:11:03Z","main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pubmed/29942048","open_access":"1"}],"publisher":"Springer Nature","date_created":"2018-12-11T11:45:07Z","publist_id":"7728","oa":1,"issue":"7","related_material":{"link":[{"url":"https://ist.ac.at/en/news/new-mechanism-for-the-plant-hormone-auxin-discovered/","description":"News on IST Homepage","relation":"press_release"}]},"abstract":[{"lang":"eng","text":"The phytohormone auxin is the information carrier in a plethora of developmental and physiological processes in plants(1). It has been firmly established that canonical, nuclear auxin signalling acts through regulation of gene transcription(2). Here, we combined microfluidics, live imaging, genetic engineering and computational modelling to reanalyse the classical case of root growth inhibition(3) by auxin. We show that Arabidopsis roots react to addition and removal of auxin by extremely rapid adaptation of growth rate. This process requires intracellular auxin perception but not transcriptional reprogramming. The formation of the canonical TIR1/AFB-Aux/IAA co-receptor complex is required for the growth regulation, hinting to a novel, non-transcriptional branch of this signalling pathway. Our results challenge the current understanding of root growth regulation by auxin and suggest another, presumably non-transcriptional, signalling output of the canonical auxin pathway."}],"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","title":"Rapid and reversible root growth inhibition by TIR1 auxin signalling","isi":1,"_id":"192","quality_controlled":"1"},{"publication_status":"published","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"doi":"10.3390/ijms19113566","type":"journal_article","license":"https://creativecommons.org/licenses/by/4.0/","article_processing_charge":"No","citation":{"short":"S. Hille, M. Akhmanova, M. Glanc, A.J. Johnson, J. Friml, International Journal of Molecular Sciences 19 (2018).","apa":"Hille, S., Akhmanova, M., Glanc, M., Johnson, A. J., & Friml, J. (2018). Relative contribution of PIN-containing secretory vesicles and plasma membrane PINs to the directed auxin transport: Theoretical estimation. International Journal of Molecular Sciences. MDPI. https://doi.org/10.3390/ijms19113566","ama":"Hille S, Akhmanova M, Glanc M, Johnson AJ, Friml J. Relative contribution of PIN-containing secretory vesicles and plasma membrane PINs to the directed auxin transport: Theoretical estimation. International Journal of Molecular Sciences. 2018;19(11). doi:10.3390/ijms19113566","chicago":"Hille, Sander, Maria Akhmanova, Matous Glanc, Alexander J Johnson, and Jiří Friml. “Relative Contribution of PIN-Containing Secretory Vesicles and Plasma Membrane PINs to the Directed Auxin Transport: Theoretical Estimation.” International Journal of Molecular Sciences. MDPI, 2018. https://doi.org/10.3390/ijms19113566.","ieee":"S. Hille, M. Akhmanova, M. Glanc, A. J. Johnson, and J. Friml, “Relative contribution of PIN-containing secretory vesicles and plasma membrane PINs to the directed auxin transport: Theoretical estimation,” International Journal of Molecular Sciences, vol. 19, no. 11. MDPI, 2018.","ista":"Hille S, Akhmanova M, Glanc M, Johnson AJ, Friml J. 2018. Relative contribution of PIN-containing secretory vesicles and plasma membrane PINs to the directed auxin transport: Theoretical estimation. International Journal of Molecular Sciences. 19(11).","mla":"Hille, Sander, et al. “Relative Contribution of PIN-Containing Secretory Vesicles and Plasma Membrane PINs to the Directed Auxin Transport: Theoretical Estimation.” International Journal of Molecular Sciences, vol. 19, no. 11, MDPI, 2018, doi:10.3390/ijms19113566."},"publication":"International Journal of Molecular Sciences","date_published":"2018-11-12T00:00:00Z","scopus_import":"1","oa_version":"Published Version","intvolume":" 19","month":"11","file_date_updated":"2020-07-14T12:44:50Z","status":"public","day":"12","year":"2018","language":[{"iso":"eng"}],"article_type":"original","has_accepted_license":"1","author":[{"full_name":"Hille, Sander","first_name":"Sander","last_name":"Hille"},{"full_name":"Akhmanova, Maria","first_name":"Maria","orcid":"0000-0003-1522-3162","last_name":"Akhmanova","id":"3425EC26-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Glanc","id":"1AE1EA24-02D0-11E9-9BAA-DAF4881429F2","full_name":"Glanc, Matous","first_name":"Matous","orcid":"0000-0003-0619-7783"},{"first_name":"Alexander J","full_name":"Johnson, Alexander J","orcid":"0000-0002-2739-8843","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","last_name":"Johnson"},{"orcid":"0000-0002-8302-7596","first_name":"Jirí","full_name":"Friml, Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"}],"volume":19,"department":[{"_id":"DaSi"},{"_id":"JiFr"}],"issue":"11","oa":1,"project":[{"call_identifier":"H2020","_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","grant_number":"742985"},{"call_identifier":"FWF","_id":"26538374-B435-11E9-9278-68D0E5697425","grant_number":"I03630","name":"Molecular mechanisms of endocytic cargo recognition in plants"}],"ec_funded":1,"ddc":["580"],"abstract":[{"lang":"eng","text":"The intercellular transport of auxin is driven by PIN-formed (PIN) auxin efflux carriers. PINs are localized at the plasma membrane (PM) and on constitutively recycling endomembrane vesicles. Therefore, PINs can mediate auxin transport either by direct translocation across the PM or by pumping auxin into secretory vesicles (SVs), leading to its secretory release upon fusion with the PM. Which of these two mechanisms dominates is a matter of debate. Here, we addressed the issue with a mathematical modeling approach. We demonstrate that the efficiency of secretory transport depends on SV size, half-life of PINs on the PM, pH, exocytosis frequency and PIN density. 3D structured illumination microscopy (SIM) was used to determine PIN density on the PM. Combining this data with published values of the other parameters, we show that the transport activity of PINs in SVs would have to be at least 1000× greater than on the PM in order to produce a comparable macroscopic auxin transport. If both transport mechanisms operated simultaneously and PINs were equally active on SVs and PM, the contribution of secretion to the total auxin flux would be negligible. In conclusion, while secretory vesicle-mediated transport of auxin is an intriguing and theoretically possible model, it is unlikely to be a major mechanism of auxin transport inplanta."}],"publication_identifier":{"eissn":["1422-0067"]},"acknowledgement":"European Research Council (ERC): 742985 to Jiri Friml; M.A. was supported by the Austrian Science Fund (FWF) (M2379-B28); AJ was supported by the Austria Science Fund (FWF): I03630 to Jiri Friml.","date_updated":"2023-09-18T08:09:32Z","external_id":{"isi":["000451528500282"]},"publist_id":"8042","date_created":"2018-12-11T11:44:09Z","publisher":"MDPI","_id":"14","quality_controlled":"1","title":"Relative contribution of PIN-containing secretory vesicles and plasma membrane PINs to the directed auxin transport: Theoretical estimation","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","isi":1,"file":[{"file_name":"2018_IJMS_Hille.pdf","content_type":"application/pdf","access_level":"open_access","file_size":2200593,"date_created":"2018-12-17T16:04:11Z","checksum":"e4b59c2599b0ca26ebf5b8434bcde94a","file_id":"5719","creator":"dernst","date_updated":"2020-07-14T12:44:50Z","relation":"main_file"}]},{"_id":"36","quality_controlled":"1","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","title":"Temperature-induced changes in the wheat phosphoproteome reveal temperature-regulated interconversion of phosphoforms","file":[{"content_type":"application/pdf","access_level":"open_access","file_name":"2018_JournalExperimBotany_Vu.pdf","creator":"dernst","file_id":"5741","relation":"main_file","date_updated":"2020-07-14T12:46:13Z","date_created":"2018-12-18T09:47:51Z","checksum":"34cb0a1611588b75bd6f4913fb4e30f1","file_size":3359316}],"isi":1,"oa":1,"issue":"19","abstract":[{"text":"Wheat (Triticum ssp.) is one of the most important human food sources. However, this crop is very sensitive to temperature changes. Specifically, processes during wheat leaf, flower, and seed development and photosynthesis, which all contribute to the yield of this crop, are affected by high temperature. While this has to some extent been investigated on physiological, developmental, and molecular levels, very little is known about early signalling events associated with an increase in temperature. Phosphorylation-mediated signalling mechanisms, which are quick and dynamic, are associated with plant growth and development, also under abiotic stress conditions. Therefore, we probed the impact of a short-term and mild increase in temperature on the wheat leaf and spikelet phosphoproteome. In total, 3822 (containing 5178 phosphosites) and 5581 phosphopeptides (containing 7023 phosphosites) were identified in leaf and spikelet samples, respectively. Following statistical analysis, the resulting data set provides the scientific community with a first large-scale plant phosphoproteome under the control of higher ambient temperature. This community resource on the high temperature-mediated wheat phosphoproteome will be valuable for future studies. Our analyses also revealed a core set of common proteins between leaf and spikelet, suggesting some level of conserved regulatory mechanisms. Furthermore, we observed temperature-regulated interconversion of phosphoforms, which probably impacts protein activity.","lang":"eng"}],"ddc":["581"],"external_id":{"isi":["000443568700010"]},"date_updated":"2023-09-19T10:00:46Z","acknowledgement":"TZ is supported by a grant from the Chinese Scholarship Council.","publisher":"Oxford University Press","date_created":"2018-12-11T11:44:17Z","publist_id":"8019","year":"2018","day":"31","language":[{"iso":"eng"}],"status":"public","has_accepted_license":"1","author":[{"last_name":"Vu","full_name":"Vu, Lam","first_name":"Lam"},{"full_name":"Zhu, Tingting","first_name":"Tingting","last_name":"Zhu"},{"id":"362BF7FE-F248-11E8-B48F-1D18A9856A87","last_name":"Verstraeten","orcid":"0000-0001-7241-2328","first_name":"Inge","full_name":"Verstraeten, Inge"},{"last_name":"Van De Cotte","first_name":"Brigitte","full_name":"Van De Cotte, Brigitte"},{"full_name":"Gevaert, Kris","first_name":"Kris","last_name":"Gevaert"},{"last_name":"De Smet","first_name":"Ive","full_name":"De Smet, Ive"}],"department":[{"_id":"JiFr"}],"volume":69,"page":"4609 - 4624","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"publication_status":"published","article_processing_charge":"No","type":"journal_article","doi":"10.1093/jxb/ery204","file_date_updated":"2020-07-14T12:46:13Z","month":"08","intvolume":" 69","scopus_import":"1","oa_version":"Published Version","date_published":"2018-08-31T00:00:00Z","publication":"Journal of Experimental Botany","citation":{"chicago":"Vu, Lam, Tingting Zhu, Inge Verstraeten, Brigitte Van De Cotte, Kris Gevaert, and Ive De Smet. “Temperature-Induced Changes in the Wheat Phosphoproteome Reveal Temperature-Regulated Interconversion of Phosphoforms.” Journal of Experimental Botany. Oxford University Press, 2018. https://doi.org/10.1093/jxb/ery204.","short":"L. Vu, T. Zhu, I. Verstraeten, B. Van De Cotte, K. Gevaert, I. De Smet, Journal of Experimental Botany 69 (2018) 4609–4624.","apa":"Vu, L., Zhu, T., Verstraeten, I., Van De Cotte, B., Gevaert, K., & De Smet, I. (2018). Temperature-induced changes in the wheat phosphoproteome reveal temperature-regulated interconversion of phosphoforms. Journal of Experimental Botany. Oxford University Press. https://doi.org/10.1093/jxb/ery204","ama":"Vu L, Zhu T, Verstraeten I, Van De Cotte B, Gevaert K, De Smet I. Temperature-induced changes in the wheat phosphoproteome reveal temperature-regulated interconversion of phosphoforms. Journal of Experimental Botany. 2018;69(19):4609-4624. doi:10.1093/jxb/ery204","ista":"Vu L, Zhu T, Verstraeten I, Van De Cotte B, Gevaert K, De Smet I. 2018. Temperature-induced changes in the wheat phosphoproteome reveal temperature-regulated interconversion of phosphoforms. Journal of Experimental Botany. 69(19), 4609–4624.","ieee":"L. Vu, T. Zhu, I. Verstraeten, B. Van De Cotte, K. Gevaert, and I. De Smet, “Temperature-induced changes in the wheat phosphoproteome reveal temperature-regulated interconversion of phosphoforms,” Journal of Experimental Botany, vol. 69, no. 19. Oxford University Press, pp. 4609–4624, 2018.","mla":"Vu, Lam, et al. “Temperature-Induced Changes in the Wheat Phosphoproteome Reveal Temperature-Regulated Interconversion of Phosphoforms.” Journal of Experimental Botany, vol. 69, no. 19, Oxford University Press, 2018, pp. 4609–24, doi:10.1093/jxb/ery204."}},{"ec_funded":1,"abstract":[{"text":"Land plants evolved from charophytic algae, among which Charophyceae possess the most complex body plans. We present the genome of Chara braunii; comparison of the genome to those of land plants identified evolutionary novelties for plant terrestrialization and land plant heritage genes. C. braunii employs unique xylan synthases for cell wall biosynthesis, a phragmoplast (cell separation) mechanism similar to that of land plants, and many phytohormones. C. braunii plastids are controlled via land-plant-like retrograde signaling, and transcriptional regulation is more elaborate than in other algae. The morphological complexity of this organism may result from expanded gene families, with three cases of particular note: genes effecting tolerance to reactive oxygen species (ROS), LysM receptor-like kinases, and transcription factors (TFs). Transcriptomic analysis of sexual reproductive structures reveals intricate control by TFs, activity of the ROS gene network, and the ancestral use of plant-like storage and stress protection proteins in the zygote.","lang":"eng"}],"oa":1,"project":[{"name":"Tracing Evolution of Auxin Transport and Polarity in Plants","grant_number":"742985","call_identifier":"H2020","_id":"261099A6-B435-11E9-9278-68D0E5697425"}],"issue":"2","date_created":"2018-12-11T11:44:53Z","publisher":"Cell Press","publist_id":"7774","date_updated":"2023-09-19T10:02:47Z","external_id":{"pmid":["30007417"],"isi":["000438482800019"]},"main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pubmed/30007417","open_access":"1"}],"acknowledgement":"In-Data-Review","quality_controlled":"1","_id":"148","isi":1,"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","title":"The Chara genome: Secondary complexity and implications for plant terrestrialization","article_processing_charge":"No","doi":"10.1016/j.cell.2018.06.033","type":"journal_article","publication_status":"published","page":"448 - 464.e24","oa_version":"Published Version","scopus_import":"1","intvolume":" 174","month":"07","citation":{"mla":"Nishiyama, Tomoaki, et al. “The Chara Genome: Secondary Complexity and Implications for Plant Terrestrialization.” Cell, vol. 174, no. 2, Cell Press, 2018, p. 448–464.e24, doi:10.1016/j.cell.2018.06.033.","ieee":"T. Nishiyama et al., “The Chara genome: Secondary complexity and implications for plant terrestrialization,” Cell, vol. 174, no. 2. Cell Press, p. 448–464.e24, 2018.","ista":"Nishiyama T, Sakayama H, De Vries J, Buschmann H, Saint Marcoux D, Ullrich K, Haas F, Vanderstraeten L, Becker D, Lang D, Vosolsobě S, Rombauts S, Wilhelmsson P, Janitza P, Kern R, Heyl A, Rümpler F, Calderón Villalobos L, Clay J, Skokan R, Toyoda A, Suzuki Y, Kagoshima H, Schijlen E, Tajeshwar N, Catarino B, Hetherington A, Saltykova A, Bonnot C, Breuninger H, Symeonidi A, Radhakrishnan G, Van Nieuwerburgh F, Deforce D, Chang C, Karol K, Hedrich R, Ulvskov P, Glöckner G, Delwiche C, Petrášek J, Van De Peer Y, Friml J, Beilby M, Dolan L, Kohara Y, Sugano S, Fujiyama A, Delaux PM, Quint M, Theissen G, Hagemann M, Harholt J, Dunand C, Zachgo S, Langdale J, Maumus F, Van Der Straeten D, Gould SB, Rensing S. 2018. The Chara genome: Secondary complexity and implications for plant terrestrialization. Cell. 174(2), 448–464.e24.","ama":"Nishiyama T, Sakayama H, De Vries J, et al. The Chara genome: Secondary complexity and implications for plant terrestrialization. Cell. 2018;174(2):448-464.e24. doi:10.1016/j.cell.2018.06.033","apa":"Nishiyama, T., Sakayama, H., De Vries, J., Buschmann, H., Saint Marcoux, D., Ullrich, K., … Rensing, S. (2018). The Chara genome: Secondary complexity and implications for plant terrestrialization. Cell. Cell Press. https://doi.org/10.1016/j.cell.2018.06.033","short":"T. Nishiyama, H. Sakayama, J. De Vries, H. Buschmann, D. Saint Marcoux, K. Ullrich, F. Haas, L. Vanderstraeten, D. Becker, D. Lang, S. Vosolsobě, S. Rombauts, P. Wilhelmsson, P. Janitza, R. Kern, A. Heyl, F. Rümpler, L. Calderón Villalobos, J. Clay, R. Skokan, A. Toyoda, Y. Suzuki, H. Kagoshima, E. Schijlen, N. Tajeshwar, B. Catarino, A. Hetherington, A. Saltykova, C. Bonnot, H. Breuninger, A. Symeonidi, G. Radhakrishnan, F. Van Nieuwerburgh, D. Deforce, C. Chang, K. Karol, R. Hedrich, P. Ulvskov, G. Glöckner, C. Delwiche, J. Petrášek, Y. Van De Peer, J. Friml, M. Beilby, L. Dolan, Y. Kohara, S. Sugano, A. Fujiyama, P.M. Delaux, M. Quint, G. Theissen, M. Hagemann, J. Harholt, C. Dunand, S. Zachgo, J. Langdale, F. Maumus, D. Van Der Straeten, S.B. Gould, S. Rensing, Cell 174 (2018) 448–464.e24.","chicago":"Nishiyama, Tomoaki, Hidetoshi Sakayama, Jan De Vries, Henrik Buschmann, Denis Saint Marcoux, Kristian Ullrich, Fabian Haas, et al. “The Chara Genome: Secondary Complexity and Implications for Plant Terrestrialization.” Cell. Cell Press, 2018. https://doi.org/10.1016/j.cell.2018.06.033."},"date_published":"2018-07-12T00:00:00Z","publication":"Cell","status":"public","day":"12","language":[{"iso":"eng"}],"year":"2018","pmid":1,"department":[{"_id":"JiFr"}],"volume":174,"author":[{"first_name":"Tomoaki","full_name":"Nishiyama, Tomoaki","last_name":"Nishiyama"},{"full_name":"Sakayama, Hidetoshi","first_name":"Hidetoshi","last_name":"Sakayama"},{"last_name":"De Vries","first_name":"Jan","full_name":"De Vries, Jan"},{"full_name":"Buschmann, Henrik","first_name":"Henrik","last_name":"Buschmann"},{"last_name":"Saint Marcoux","full_name":"Saint Marcoux, Denis","first_name":"Denis"},{"last_name":"Ullrich","full_name":"Ullrich, Kristian","first_name":"Kristian"},{"first_name":"Fabian","full_name":"Haas, Fabian","last_name":"Haas"},{"last_name":"Vanderstraeten","full_name":"Vanderstraeten, Lisa","first_name":"Lisa"},{"full_name":"Becker, Dirk","first_name":"Dirk","last_name":"Becker"},{"first_name":"Daniel","full_name":"Lang, Daniel","last_name":"Lang"},{"last_name":"Vosolsobě","full_name":"Vosolsobě, Stanislav","first_name":"Stanislav"},{"first_name":"Stephane","full_name":"Rombauts, Stephane","last_name":"Rombauts"},{"full_name":"Wilhelmsson, Per","first_name":"Per","last_name":"Wilhelmsson"},{"last_name":"Janitza","first_name":"Philipp","full_name":"Janitza, Philipp"},{"last_name":"Kern","first_name":"Ramona","full_name":"Kern, Ramona"},{"first_name":"Alexander","full_name":"Heyl, Alexander","last_name":"Heyl"},{"last_name":"Rümpler","full_name":"Rümpler, Florian","first_name":"Florian"},{"last_name":"Calderón Villalobos","first_name":"Luz","full_name":"Calderón Villalobos, Luz"},{"full_name":"Clay, John","first_name":"John","last_name":"Clay"},{"first_name":"Roman","full_name":"Skokan, Roman","last_name":"Skokan"},{"full_name":"Toyoda, Atsushi","first_name":"Atsushi","last_name":"Toyoda"},{"last_name":"Suzuki","full_name":"Suzuki, Yutaka","first_name":"Yutaka"},{"last_name":"Kagoshima","first_name":"Hiroshi","full_name":"Kagoshima, Hiroshi"},{"last_name":"Schijlen","first_name":"Elio","full_name":"Schijlen, Elio"},{"first_name":"Navindra","full_name":"Tajeshwar, Navindra","last_name":"Tajeshwar"},{"last_name":"Catarino","first_name":"Bruno","full_name":"Catarino, Bruno"},{"first_name":"Alexander","full_name":"Hetherington, Alexander","last_name":"Hetherington"},{"first_name":"Assia","full_name":"Saltykova, Assia","last_name":"Saltykova"},{"first_name":"Clemence","full_name":"Bonnot, Clemence","last_name":"Bonnot"},{"first_name":"Holger","full_name":"Breuninger, Holger","last_name":"Breuninger"},{"first_name":"Aikaterini","full_name":"Symeonidi, Aikaterini","last_name":"Symeonidi"},{"first_name":"Guru","full_name":"Radhakrishnan, Guru","last_name":"Radhakrishnan"},{"full_name":"Van Nieuwerburgh, Filip","first_name":"Filip","last_name":"Van Nieuwerburgh"},{"first_name":"Dieter","full_name":"Deforce, Dieter","last_name":"Deforce"},{"first_name":"Caren","full_name":"Chang, Caren","last_name":"Chang"},{"last_name":"Karol","first_name":"Kenneth","full_name":"Karol, Kenneth"},{"last_name":"Hedrich","first_name":"Rainer","full_name":"Hedrich, Rainer"},{"last_name":"Ulvskov","full_name":"Ulvskov, Peter","first_name":"Peter"},{"full_name":"Glöckner, Gernot","first_name":"Gernot","last_name":"Glöckner"},{"last_name":"Delwiche","full_name":"Delwiche, Charles","first_name":"Charles"},{"last_name":"Petrášek","first_name":"Jan","full_name":"Petrášek, Jan"},{"first_name":"Yves","full_name":"Van De Peer, Yves","last_name":"Van De Peer"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","first_name":"Jirí"},{"last_name":"Beilby","first_name":"Mary","full_name":"Beilby, Mary"},{"first_name":"Liam","full_name":"Dolan, Liam","last_name":"Dolan"},{"last_name":"Kohara","first_name":"Yuji","full_name":"Kohara, Yuji"},{"last_name":"Sugano","full_name":"Sugano, Sumio","first_name":"Sumio"},{"full_name":"Fujiyama, Asao","first_name":"Asao","last_name":"Fujiyama"},{"full_name":"Delaux, Pierre Marc","first_name":"Pierre Marc","last_name":"Delaux"},{"full_name":"Quint, Marcel","first_name":"Marcel","last_name":"Quint"},{"last_name":"Theissen","full_name":"Theissen, Gunter","first_name":"Gunter"},{"full_name":"Hagemann, Martin","first_name":"Martin","last_name":"Hagemann"},{"first_name":"Jesper","full_name":"Harholt, Jesper","last_name":"Harholt"},{"full_name":"Dunand, Christophe","first_name":"Christophe","last_name":"Dunand"},{"last_name":"Zachgo","full_name":"Zachgo, Sabine","first_name":"Sabine"},{"full_name":"Langdale, Jane","first_name":"Jane","last_name":"Langdale"},{"last_name":"Maumus","full_name":"Maumus, Florian","first_name":"Florian"},{"full_name":"Van Der Straeten, Dominique","first_name":"Dominique","last_name":"Van Der Straeten"},{"first_name":"Sven B","full_name":"Gould, Sven B","last_name":"Gould"},{"first_name":"Stefan","full_name":"Rensing, Stefan","last_name":"Rensing"}]},{"quality_controlled":"1","_id":"147","isi":1,"title":"The inhibitor Endosidin 4 targets SEC7 domain-type ARF GTPase exchange factors and interferes with sub cellular trafficking in eukaryotes","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","ec_funded":1,"abstract":[{"lang":"eng","text":"The trafficking of subcellular cargos in eukaryotic cells crucially depends on vesicle budding, a process mediated by ARF-GEFs (ADP-ribosylation factor guanine nucleotide exchange factors). In plants, ARF-GEFs play essential roles in endocytosis, vacuolar trafficking, recycling, secretion, and polar trafficking. Moreover, they are important for plant development, mainly through controlling the polar subcellular localization of PIN-FORMED (PIN) transporters of the plant hormone auxin. Here, using a chemical genetics screen in Arabidopsis thaliana, we identified Endosidin 4 (ES4), an inhibitor of eukaryotic ARF-GEFs. ES4 acts similarly to and synergistically with the established ARF-GEF inhibitor Brefeldin A and has broad effects on intracellular trafficking, including endocytosis, exocytosis, and vacuolar targeting. Additionally, Arabidopsis and yeast (Sacharomyces cerevisiae) mutants defective in ARF-GEF show altered sensitivity to ES4. ES4 interferes with the activation-based membrane association of the ARF1 GTPases, but not of their mutant variants that are activated independently of ARF-GEF activity. Biochemical approaches and docking simulations confirmed that ES4 specifically targets the SEC7 domain-containing ARF-GEFs. These observations collectively identify ES4 as a chemical tool enabling the study of ARF-GEF-mediated processes, including ARF-GEF-mediated plant development."}],"publication_identifier":{"issn":["1040-4651"]},"issue":"10","oa":1,"project":[{"grant_number":"282300","name":"Polarity and subcellular dynamics in plants","call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425"},{"_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","grant_number":"742985"}],"publist_id":"7776","date_created":"2018-12-11T11:44:52Z","publisher":"Oxford University Press","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1105/tpc.18.00127"}],"acknowledgement":"We thank Gerd Jürgens, Sandra Richter, and Sheng Yang He for providing antibodies; Maciek Adamowski, Fernando Aniento, Sebastian Bednarek, Nico Callewaert, Matyás Fendrych, Elena Feraru, and Mugurel I. Feraru for helpful suggestions; Siamsa Doyle for critical reading of the manuscript and helpful comments and suggestions; and Stephanie Smith and Martine De Cock for help in editing and language corrections. We acknowledge the core facility Cellular Imaging of CEITEC supported by the Czech-BioImaging large RI project (LM2015062 funded by MEYS CR) for their support with obtaining scientific data presented in this article. Plant Sciences Core Facility of CEITEC Masaryk University is gratefully acknowledged for obtaining part of the scientific data presented in this article. We acknowledge support from the Fondation pour la Recherche Médicale and from the Institut National du Cancer (J.C.). The research leading to these results was funded by the European Research Council under the European Union's 7th Framework Program (FP7/2007-2013)/ERC grant agreement numbers 282300 and 742985 and the Czech Science Foundation GAČR (GA18-26981S; J.F.); Ministry of Education, Youth, and Sports/MEYS of the Czech Republic under the Project CEITEC 2020 (LQ1601; T.N.); the China Science Council for a predoctoral fellowship (Q.L.); a joint research project within the framework of cooperation between the Research Foundation-Flanders and the Bulgarian Academy of Sciences (VS.025.13N; K.M. and E.R.); Vetenskapsrådet and Vinnova (Verket för Innovationssystem; S.R.), Knut och Alice Wallenbergs Stiftelse via “Shapesystem” Grant 2012.0050 (S.R.), Kempe stiftelserna (P.G.), Tryggers CTS410 (P.G.).","date_updated":"2023-09-19T10:09:12Z","external_id":{"isi":["000450000500023"],"pmid":["30018156"]},"article_type":"original","status":"public","year":"2018","language":[{"iso":"eng"}],"day":"12","volume":30,"department":[{"_id":"JiFr"}],"pmid":1,"author":[{"full_name":"Kania, Urszula","first_name":"Urszula","last_name":"Kania","id":"4AE5C486-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Tomasz","full_name":"Nodzyński, Tomasz","last_name":"Nodzyński"},{"last_name":"Lu","first_name":"Qing","full_name":"Lu, Qing"},{"full_name":"Hicks, Glenn R","first_name":"Glenn R","last_name":"Hicks"},{"last_name":"Nerinckx","full_name":"Nerinckx, Wim","first_name":"Wim"},{"full_name":"Mishev, Kiril","first_name":"Kiril","last_name":"Mishev"},{"last_name":"Peurois","full_name":"Peurois, Francois","first_name":"Francois"},{"last_name":"Cherfils","first_name":"Jacqueline","full_name":"Cherfils, Jacqueline"},{"first_name":"Rycke Riet Maria","full_name":"De, Rycke Riet Maria","last_name":"De"},{"id":"399876EC-F248-11E8-B48F-1D18A9856A87","last_name":"Grones","first_name":"Peter","full_name":"Grones, Peter"},{"first_name":"Stéphanie","full_name":"Robert, Stéphanie","last_name":"Robert"},{"last_name":"Russinova","full_name":"Russinova, Eugenia","first_name":"Eugenia"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"type":"journal_article","doi":"10.1105/tpc.18.00127","article_processing_charge":"No","publication_status":"published","page":"2553 - 2572","citation":{"mla":"Kania, Urszula, et al. “The Inhibitor Endosidin 4 Targets SEC7 Domain-Type ARF GTPase Exchange Factors and Interferes with Sub Cellular Trafficking in Eukaryotes.” The Plant Cell, vol. 30, no. 10, Oxford University Press, 2018, pp. 2553–72, doi:10.1105/tpc.18.00127.","ieee":"U. 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The inhibitor Endosidin 4 targets SEC7 domain-type ARF GTPase exchange factors and interferes with sub cellular trafficking in eukaryotes. The Plant Cell. Oxford University Press. https://doi.org/10.1105/tpc.18.00127","short":"U. Kania, T. Nodzyński, Q. Lu, G.R. Hicks, W. Nerinckx, K. Mishev, F. Peurois, J. Cherfils, R.R.M. De, P. Grones, S. Robert, E. Russinova, J. Friml, The Plant Cell 30 (2018) 2553–2572.","chicago":"Kania, Urszula, Tomasz Nodzyński, Qing Lu, Glenn R Hicks, Wim Nerinckx, Kiril Mishev, Francois Peurois, et al. “The Inhibitor Endosidin 4 Targets SEC7 Domain-Type ARF GTPase Exchange Factors and Interferes with Sub Cellular Trafficking in Eukaryotes.” The Plant Cell. Oxford University Press, 2018. https://doi.org/10.1105/tpc.18.00127."},"date_published":"2018-11-12T00:00:00Z","publication":"The Plant Cell","oa_version":"Published Version","scopus_import":"1","intvolume":" 30","month":"11"},{"language":[{"iso":"eng"}],"day":"30","year":"2018","status":"public","has_accepted_license":"1","article_type":"original","author":[{"last_name":"Shi","first_name":"Chun Lin","full_name":"Shi, Chun Lin"},{"last_name":"Von Wangenheim","id":"49E91952-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6862-1247","full_name":"Von Wangenheim, Daniel","first_name":"Daniel"},{"last_name":"Herrmann","first_name":"Ullrich","full_name":"Herrmann, Ullrich"},{"last_name":"Wildhagen","full_name":"Wildhagen, Mari","first_name":"Mari"},{"last_name":"Kulik","id":"F0AB3FCE-02D1-11E9-BD0E-99399A5D3DEB","full_name":"Kulik, Ivan","first_name":"Ivan"},{"full_name":"Kopf, Andreas","first_name":"Andreas","last_name":"Kopf"},{"last_name":"Ishida","full_name":"Ishida, Takashi","first_name":"Takashi"},{"first_name":"Vilde","full_name":"Olsson, Vilde","last_name":"Olsson"},{"last_name":"Anker","first_name":"Mari Kristine","full_name":"Anker, Mari Kristine"},{"last_name":"Albert","full_name":"Albert, Markus","first_name":"Markus"},{"last_name":"Butenko","first_name":"Melinka A","full_name":"Butenko, Melinka A"},{"last_name":"Felix","first_name":"Georg","full_name":"Felix, Georg"},{"full_name":"Sawa, Shinichiro","first_name":"Shinichiro","last_name":"Sawa"},{"last_name":"Claassen","first_name":"Manfred","full_name":"Claassen, Manfred"},{"first_name":"Jirí","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"},{"last_name":"Aalen","full_name":"Aalen, Reidunn B","first_name":"Reidunn B"}],"pmid":1,"department":[{"_id":"JiFr"}],"volume":4,"page":"596 - 604","publication_status":"published","article_processing_charge":"No","type":"journal_article","doi":"10.1038/s41477-018-0212-z","intvolume":" 4","file_date_updated":"2020-07-14T12:44:56Z","month":"07","oa_version":"Submitted Version","scopus_import":"1","date_published":"2018-07-30T00:00:00Z","publication":"Nature Plants","citation":{"ieee":"C. L. Shi et al., “The dynamics of root cap sloughing in Arabidopsis is regulated by peptide signalling,” Nature Plants, vol. 4, no. 8. Nature Publishing Group, pp. 596–604, 2018.","mla":"Shi, Chun Lin, et al. “The Dynamics of Root Cap Sloughing in Arabidopsis Is Regulated by Peptide Signalling.” Nature Plants, vol. 4, no. 8, Nature Publishing Group, 2018, pp. 596–604, doi:10.1038/s41477-018-0212-z.","ista":"Shi CL, von Wangenheim D, Herrmann U, Wildhagen M, Kulik I, Kopf A, Ishida T, Olsson V, Anker MK, Albert M, Butenko MA, Felix G, Sawa S, Claassen M, Friml J, Aalen RB. 2018. The dynamics of root cap sloughing in Arabidopsis is regulated by peptide signalling. Nature Plants. 4(8), 596–604.","chicago":"Shi, Chun Lin, Daniel von Wangenheim, Ullrich Herrmann, Mari Wildhagen, Ivan Kulik, Andreas Kopf, Takashi Ishida, et al. “The Dynamics of Root Cap Sloughing in Arabidopsis Is Regulated by Peptide Signalling.” Nature Plants. Nature Publishing Group, 2018. https://doi.org/10.1038/s41477-018-0212-z.","apa":"Shi, C. L., von Wangenheim, D., Herrmann, U., Wildhagen, M., Kulik, I., Kopf, A., … Aalen, R. B. (2018). The dynamics of root cap sloughing in Arabidopsis is regulated by peptide signalling. Nature Plants. Nature Publishing Group. https://doi.org/10.1038/s41477-018-0212-z","ama":"Shi CL, von Wangenheim D, Herrmann U, et al. The dynamics of root cap sloughing in Arabidopsis is regulated by peptide signalling. Nature Plants. 2018;4(8):596-604. doi:10.1038/s41477-018-0212-z","short":"C.L. Shi, D. von Wangenheim, U. Herrmann, M. Wildhagen, I. Kulik, A. Kopf, T. Ishida, V. Olsson, M.K. Anker, M. Albert, M.A. Butenko, G. Felix, S. Sawa, M. Claassen, J. Friml, R.B. Aalen, Nature Plants 4 (2018) 596–604."},"_id":"146","quality_controlled":"1","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","title":"The dynamics of root cap sloughing in Arabidopsis is regulated by peptide signalling","file":[{"file_id":"7043","creator":"dernst","relation":"main_file","date_updated":"2020-07-14T12:44:56Z","file_size":226829,"checksum":"da33101c76ee1b2dc5ab28fd2ccba9d0","date_created":"2019-11-18T16:24:07Z","content_type":"application/pdf","access_level":"open_access","file_name":"2018_NaturePlants_Shi.pdf"}],"isi":1,"oa":1,"issue":"8","related_material":{"link":[{"url":"https://ist.ac.at/en/news/new-process-in-root-development-discovered/","relation":"press_release","description":"News on IST Homepage"}]},"ddc":["580"],"abstract":[{"text":"The root cap protects the stem cell niche of angiosperm roots from damage. In Arabidopsis, lateral root cap (LRC) cells covering the meristematic zone are regularly lost through programmed cell death, while the outermost layer of the root cap covering the tip is repeatedly sloughed. Efficient coordination with stem cells producing new layers is needed to maintain a constant size of the cap. We present a signalling pair, the peptide IDA-LIKE1 (IDL1) and its receptor HAESA-LIKE2 (HSL2), mediating such communication. Live imaging over several days characterized this process from initial fractures in LRC cell files to full separation of a layer. Enhanced expression of IDL1 in the separating root cap layers resulted in increased frequency of sloughing, balanced with generation of new layers in a HSL2-dependent manner. Transcriptome analyses linked IDL1-HSL2 signalling to the transcription factors BEARSKIN1/2 and genes associated with programmed cell death. Mutations in either IDL1 or HSL2 slowed down cell division, maturation and separation. Thus, IDL1-HSL2 signalling potentiates dynamic regulation of the homeostatic balance between stem cell division and sloughing activity.","lang":"eng"}],"external_id":{"isi":["000443861300016"],"pmid":["30061750"]},"date_updated":"2023-09-19T10:08:45Z","publisher":"Nature Publishing Group","date_created":"2018-12-11T11:44:52Z","publist_id":"7777"},{"department":[{"_id":"JiFr"}],"pmid":1,"volume":69,"author":[{"first_name":"Taraka Ramji","full_name":"Moturu, Taraka Ramji","last_name":"Moturu"},{"last_name":"Thula","full_name":"Thula, Sravankumar","first_name":"Sravankumar"},{"first_name":"Ravi Kumar","full_name":"Singh, Ravi Kumar","last_name":"Singh"},{"last_name":"Nodzyński","first_name":"Tomasz","full_name":"Nodzyński, Tomasz"},{"last_name":"Vařeková","first_name":"Radka Svobodová","full_name":"Vařeková, Radka Svobodová"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","first_name":"Jiří","orcid":"0000-0002-8302-7596"},{"last_name":"Simon","first_name":"Sibu","full_name":"Simon, Sibu"}],"article_type":"original","status":"public","language":[{"iso":"eng"}],"day":"13","year":"2018","scopus_import":"1","oa_version":"None","intvolume":" 69","month":"04","citation":{"short":"T.R. Moturu, S. Thula, R.K. Singh, T. Nodzyński, R.S. Vařeková, J. Friml, S. Simon, Journal of Experimental Botany 69 (2018) 2367–2378.","apa":"Moturu, T. R., Thula, S., Singh, R. K., Nodzyński, T., Vařeková, R. S., Friml, J., & Simon, S. (2018). Molecular evolution and diversification of the SMXL gene family. Journal of Experimental Botany. Oxford University Press. https://doi.org/10.1093/jxb/ery097","ama":"Moturu TR, Thula S, Singh RK, et al. Molecular evolution and diversification of the SMXL gene family. Journal of Experimental Botany. 2018;69(9):2367-2378. doi:10.1093/jxb/ery097","chicago":"Moturu, Taraka Ramji, Sravankumar Thula, Ravi Kumar Singh, Tomasz Nodzyński, Radka Svobodová Vařeková, Jiří Friml, and Sibu Simon. “Molecular Evolution and Diversification of the SMXL Gene Family.” Journal of Experimental Botany. Oxford University Press, 2018. https://doi.org/10.1093/jxb/ery097.","ieee":"T. R. Moturu et al., “Molecular evolution and diversification of the SMXL gene family,” Journal of Experimental Botany, vol. 69, no. 9. Oxford University Press, pp. 2367–2378, 2018.","ista":"Moturu TR, Thula S, Singh RK, Nodzyński T, Vařeková RS, Friml J, Simon S. 2018. Molecular evolution and diversification of the SMXL gene family. Journal of Experimental Botany. 69(9), 2367–2378.","mla":"Moturu, Taraka Ramji, et al. “Molecular Evolution and Diversification of the SMXL Gene Family.” Journal of Experimental Botany, vol. 69, no. 9, Oxford University Press, 2018, pp. 2367–78, doi:10.1093/jxb/ery097."},"publication":"Journal of Experimental Botany","date_published":"2018-04-13T00:00:00Z","article_processing_charge":"No","type":"journal_article","doi":"10.1093/jxb/ery097","publication_status":"published","page":"2367-2378","isi":1,"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","title":"Molecular evolution and diversification of the SMXL gene family","keyword":["Plant Science","Physiology"],"quality_controlled":"1","_id":"10881","date_created":"2022-03-18T12:43:22Z","publisher":"Oxford University Press","date_updated":"2023-09-19T15:10:43Z","external_id":{"pmid":["29538714"],"isi":["000430727000016"]},"acknowledgement":"This project received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Actions and it is co-financed by the South Moravian Region under grant agreement No. 665860 (SS). Access to computing and storage facilities owned by parties and projects contributing to the national grid infrastructure, MetaCentrum, provided under the program ‘Projects of Large Infrastructure for Research, Development, and Innovations’ (LM2010005) was greatly appreciated (RSV). The project was funded by The Ministry of Education, Youth and Sports/MES of the Czech Republic under the project CEITEC 2020 (LQ1601) (TN, TRM). JF was supported by the European Research Council (project ERC-2011-StG 20101109-PSDP) and the Czech Science Foundation GAČR (GA13-40637S). We thank Dr Kamel Chibani for active discussions on the evolutionary analysis and Nandan Mysore Vardarajan for his critical comments on the manuscript. This article reflects\r\nonly the authors’ views, and the EU is not responsible for any use that may be made of the information it contains. ","publication_identifier":{"issn":["0022-0957"],"eissn":["1460-2431"]},"ec_funded":1,"abstract":[{"lang":"eng","text":"Strigolactones (SLs) are a relatively recent addition to the list of plant hormones that control different aspects of plant development. SL signalling is perceived by an α/β hydrolase, DWARF 14 (D14). A close homolog of D14, KARRIKIN INSENSTIVE2 (KAI2), is involved in perception of an uncharacterized molecule called karrikin (KAR). Recent studies in Arabidopsis identified the SUPPRESSOR OF MAX2 1 (SMAX1) and SMAX1-LIKE 7 (SMXL7) to be potential SCF–MAX2 complex-mediated proteasome targets of KAI2 and D14, respectively. Genetic studies on SMXL7 and SMAX1 demonstrated distinct developmental roles for each, but very little is known about these repressors in terms of their sequence features. In this study, we performed an extensive comparative analysis of SMXLs and determined their phylogenetic and evolutionary history in the plant lineage. Our results show that SMXL family members can be sub-divided into four distinct phylogenetic clades/classes, with an ancient SMAX1. Further, we identified the clade-specific motifs that have evolved and that might act as determinants of SL-KAR signalling specificity. These specificities resulted from functional diversities among the clades. Our results suggest that a gradual co-evolution of SMXL members with their upstream receptors D14/KAI2 provided an increased specificity to both the SL perception and response in land plants."}],"project":[{"_id":"25716A02-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","name":"Polarity and subcellular dynamics in plants","grant_number":"282300"}],"issue":"9"}]