@article{7889,
abstract = {Autoluminescent plants engineered to express a bacterial bioluminescence gene cluster in plastids have not been widely adopted because of low light output. We engineered tobacco plants with a fungal bioluminescence system that converts caffeic acid (present in all plants) into luciferin and report self-sustained luminescence that is visible to the naked eye. Our findings could underpin development of a suite of imaging tools for plants.},
author = {Mitiouchkina, Tatiana and Mishin, Alexander S. and Gonzalez Somermeyer, Louisa and Markina, Nadezhda M. and Chepurnyh, Tatiana V. and Guglya, Elena B. and Karataeva, Tatiana A. and Palkina, Kseniia A. and Shakhova, Ekaterina S. and Fakhranurova, Liliia I. and Chekova, Sofia V. and Tsarkova, Aleksandra S. and Golubev, Yaroslav V. and Negrebetsky, Vadim V. and Dolgushin, Sergey A. and Shalaev, Pavel V. and Shlykov, Dmitry and Melnik, Olesya A. and Shipunova, Victoria O. and Deyev, Sergey M. and Bubyrev, Andrey I. and Pushin, Alexander S. and Choob, Vladimir V. and Dolgov, Sergey V. and Kondrashov, Fyodor and Yampolsky, Ilia V. and Sarkisyan, Karen S.},
issn = {1087-0156},
journal = {Nature Biotechnology},
pages = {944--946},
publisher = {Springer Nature},
title = {{Plants with genetically encoded autoluminescence}},
doi = {10.1038/s41587-020-0500-9},
volume = {38},
year = {2020},
}
@article{7900,
abstract = {Hartree–Fock theory has been justified as a mean-field approximation for fermionic systems. However, it suffers from some defects in predicting physical properties, making necessary a theory of quantum correlations. Recently, bosonization of many-body correlations has been rigorously justified as an upper bound on the correlation energy at high density with weak interactions. We review the bosonic approximation, deriving an effective Hamiltonian. We then show that for systems with Coulomb interaction this effective theory predicts collective excitations (plasmons) in accordance with the random phase approximation of Bohm and Pines, and with experimental observation.},
author = {Benedikter, Niels P},
issn = {0129-055X},
journal = {Reviews in Mathematical Physics},
publisher = {World Scientific},
title = {{Bosonic collective excitations in Fermi gases}},
doi = {10.1142/s0129055x20600090},
year = {2020},
}
@article{7908,
abstract = {Volatile anesthetics are widely used for surgery, but neuronal mechanisms of anesthesia remain unidentified. At the calyx of Held in brainstem slices from rats of either sex, isoflurane at clinical doses attenuated EPSCs by decreasing the release probability and the number of readily releasable vesicles. In presynaptic recordings of Ca2+ currents and exocytic capacitance changes, isoflurane attenuated exocytosis by inhibiting Ca2+ currents evoked by a short presynaptic depolarization, whereas it inhibited exocytosis evoked by a prolonged depolarization via directly blocking exocytic machinery downstream of Ca2+ influx. Since the length of presynaptic depolarization can simulate the frequency of synaptic inputs, isoflurane anesthesia is likely mediated by distinct dual mechanisms, depending on input frequencies. In simultaneous presynaptic and postsynaptic action potential recordings, isoflurane impaired the fidelity of repetitive spike transmission, more strongly at higher frequencies. Furthermore, in the cerebrum of adult mice, isoflurane inhibited monosynaptic corticocortical spike transmission, preferentially at a higher frequency. We conclude that dual presynaptic mechanisms operate for the anesthetic action of isoflurane, of which direct inhibition of exocytic machinery plays a low-pass filtering role in spike transmission at central excitatory synapses.},
author = {Wang, Han Ying and Eguchi, Kohgaku and Yamashita, Takayuki and Takahashi, Tomoyuki},
issn = {15292401},
journal = {Journal of Neuroscience},
number = {21},
pages = {4103--4115},
publisher = {Society for Neuroscience},
title = {{Frequency-dependent block of excitatory neurotransmission by isoflurane via dual presynaptic mechanisms}},
doi = {10.1523/JNEUROSCI.2946-19.2020},
volume = {40},
year = {2020},
}
@article{7909,
abstract = {Cell migration entails networks and bundles of actin filaments termed lamellipodia and microspikes or filopodia, respectively, as well as focal adhesions, all of which recruit Ena/VASP family members hitherto thought to antagonize efficient cell motility. However, we find these proteins to act as positive regulators of migration in different murine cell lines. CRISPR/Cas9-mediated loss of Ena/VASP proteins reduced lamellipodial actin assembly and perturbed lamellipodial architecture, as evidenced by changed network geometry as well as reduction of filament length and number that was accompanied by abnormal Arp2/3 complex and heterodimeric capping protein accumulation. Loss of Ena/VASP function also abolished the formation of microspikes normally embedded in lamellipodia, but not of filopodia capable of emanating without lamellipodia. Ena/VASP-deficiency also impaired integrin-mediated adhesion accompanied by reduced traction forces exerted through these structures. Our data thus uncover novel Ena/VASP functions of these actin polymerases that are fully consistent with their promotion of cell migration.},
author = {Damiano-Guercio, Julia and Kurzawa, Laëtitia and Müller, Jan and Dimchev, Georgi A and Schaks, Matthias and Nemethova, Maria and Pokrant, Thomas and Brühmann, Stefan and Linkner, Joern and Blanchoin, Laurent and Sixt, Michael K and Rottner, Klemens and Faix, Jan},
issn = {2050084X},
journal = {eLife},
publisher = {eLife Sciences Publications},
title = {{Loss of Ena/VASP interferes with lamellipodium architecture, motility and integrin-dependent adhesion}},
doi = {10.7554/eLife.55351},
volume = {9},
year = {2020},
}
@article{7910,
abstract = {Quantum illumination uses entangled signal-idler photon pairs to boost the detection efficiency of low-reflectivity objects in environments with bright thermal noise. Its advantage is particularly evident at low signal powers, a promising feature for applications such as noninvasive biomedical scanning or low-power short-range radar. Here, we experimentally investigate the concept of quantum illumination at microwave frequencies. We generate entangled fields to illuminate a room-temperature object at a distance of 1 m in a free-space detection setup. We implement a digital phase-conjugate receiver based on linear quadrature measurements that outperforms a symmetric classical noise radar in the same conditions, despite the entanglement-breaking signal path. Starting from experimental data, we also simulate the case of perfect idler photon number detection, which results in a quantum advantage compared with the relative classical benchmark. Our results highlight the opportunities and challenges in the way toward a first room-temperature application of microwave quantum circuits.},
author = {Barzanjeh, Shabir and Pirandola, S. and Vitali, D and Fink, Johannes M},
issn = {23752548},
journal = {Science Advances},
number = {19},
publisher = {AAAS},
title = {{Microwave quantum illumination using a digital receiver}},
doi = {10.1126/sciadv.abb0451},
volume = {6},
year = {2020},
}
@article{7919,
abstract = {We explore the time evolution of two impurities in a trapped one-dimensional Bose gas that follows a change of the boson-impurity interaction. We study the induced impurity-impurity interactions and their effect on the quench dynamics. In particular, we report on the size of the impurity cloud, the impurity-impurity entanglement, and the impurity-impurity correlation function. The presented numerical simulations are based upon the variational multilayer multiconfiguration time-dependent Hartree method for bosons. To analyze and quantify induced impurity-impurity correlations, we employ an effective two-body Hamiltonian with a contact interaction. We show that the effective model consistent with the mean-field attraction of two heavy impurities explains qualitatively our results for weak interactions. Our findings suggest that the quench dynamics in cold-atom systems can be a tool for studying impurity-impurity correlations.},
author = {Mistakidis, S. I. and Volosniev, Artem and Schmelcher, P.},
issn = {2643-1564},
journal = {Physical Review Research},
publisher = {American Physical Society},
title = {{Induced correlations between impurities in a one-dimensional quenched Bose gas}},
doi = {10.1103/physrevresearch.2.023154},
volume = {2},
year = {2020},
}
@article{7931,
abstract = {In the course of sample preparation for Next Generation Sequencing (NGS), DNA is fragmented by various methods. Fragmentation shows a persistent bias with regard to the cleavage rates of various dinucleotides. With the exception of CpG dinucleotides the previously described biases were consistent with results of the DNA cleavage in solution. Here we computed cleavage rates of all dinucleotides including the methylated CpG and unmethylated CpG dinucleotides using data of the Whole Genome Sequencing datasets of the 1000 Genomes project. We found that the cleavage rate of CpG is significantly higher for the methylated CpG dinucleotides. Using this information, we developed a classifier for distinguishing cancer and healthy tissues based on their CpG islands statuses of the fragmentation. A simple Support Vector Machine classifier based on this algorithm shows an accuracy of 84%. The proposed method allows the detection of epigenetic markers purely based on mechanochemical DNA fragmentation, which can be detected by a simple analysis of the NGS sequencing data.},
author = {Uroshlev, Leonid A. and Abdullaev, Eldar T. and Umarova, Iren R. and Il’Icheva, Irina A. and Panchenko, Larisa A. and Polozov, Robert V. and Kondrashov, Fyodor and Nechipurenko, Yury D. and Grokhovsky, Sergei L.},
issn = {20452322},
journal = {Scientific Reports},
publisher = {Springer Nature},
title = {{A method for identification of the methylation level of CpG islands from NGS data}},
doi = {10.1038/s41598-020-65406-1},
volume = {10},
year = {2020},
}
@article{7932,
abstract = {Pulsating flows through tubular geometries are laminar provided that velocities are moderate. This in particular is also believed to apply to cardiovascular flows where inertial forces are typically too low to sustain turbulence. On the other hand, flow instabilities and fluctuating shear stresses are held responsible for a variety of cardiovascular diseases. Here we report a nonlinear instability mechanism for pulsating pipe flow that gives rise to bursts of turbulence at low flow rates. Geometrical distortions of small, yet finite, amplitude are found to excite a state consisting of helical vortices during flow deceleration. The resulting flow pattern grows rapidly in magnitude, breaks down into turbulence, and eventually returns to laminar when the flow accelerates. This scenario causes shear stress fluctuations and flow reversal during each pulsation cycle. Such unsteady conditions can adversely affect blood vessels and have been shown to promote inflammation and dysfunction of the shear stress-sensitive endothelial cell layer.},
author = {Xu, Duo and Varshney, Atul and Ma, Xingyu and Song, Baofang and Riedl, Michael and Avila, Marc and Hof, Björn},
issn = {10916490},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = {21},
pages = {11233--11239},
publisher = {National Academy of Sciences},
title = {{Nonlinear hydrodynamic instability and turbulence in pulsatile flow}},
doi = {10.1073/pnas.1913716117},
volume = {117},
year = {2020},
}
@article{7933,
abstract = {We study a mobile quantum impurity, possessing internal rotational degrees of freedom, confined to a ring in the presence of a many-particle bosonic bath. By considering the recently introduced rotating polaron problem, we define the Hamiltonian and examine the energy spectrum. The weak-coupling regime is studied by means of a variational ansatz in the truncated Fock space. The corresponding spectrum indicates that there emerges a coupling between the internal and orbital angular momenta of the impurity as a consequence of the phonon exchange. We interpret the coupling as a phonon-mediated spin-orbit coupling and quantify it by using a correlation function between the internal and the orbital angular momentum operators. The strong-coupling regime is investigated within the Pekar approach, and it is shown that the correlation function of the ground state shows a kink at a critical coupling, that is explained by a sharp transition from the noninteracting state to the states that exhibit strong interaction with the surroundings. The results might find applications in such fields as spintronics or topological insulators where spin-orbit coupling is of crucial importance.},
author = {Maslov, Mikhail and Lemeshko, Mikhail and Yakaboylu, Enderalp},
issn = {24699969},
journal = {Physical Review B},
number = {18},
publisher = {American Physical Society},
title = {{Synthetic spin-orbit coupling mediated by a bosonic environment}},
doi = {10.1103/PhysRevB.101.184104},
volume = {101},
year = {2020},
}
@inbook{7941,
abstract = {Expansion microscopy is a recently developed super-resolution imaging technique, which provides an alternative to optics-based methods such as deterministic approaches (e.g. STED) or stochastic approaches (e.g. PALM/STORM). The idea behind expansion microscopy is to embed the biological sample in a swellable gel, and then to expand it isotropically, thereby increasing the distance between the fluorophores. This approach breaks the diffraction barrier by simply separating the emission point-spread-functions of the fluorophores. The resolution attainable in expansion microscopy is thus directly dependent on the separation that can be achieved, i.e. on the expansion factor. The original implementation of the technique achieved an expansion factor of fourfold, for a resolution of 70–80 nm. The subsequently developed X10 method achieves an expansion factor of 10-fold, for a resolution of 25–30 nm. This technique can be implemented with minimal technical requirements on any standard fluorescence microscope, and is more easily applied for multi-color imaging than either deterministic or stochastic super-resolution approaches. This renders X10 expansion microscopy a highly promising tool for new biological discoveries, as discussed here, and as demonstrated by several recent applications.},
author = {Truckenbrodt, Sven M and Rizzoli, Silvio O.},
booktitle = {Methods in Cell Biology},
issn = {0091679X},
publisher = {Elsevier},
title = {{Simple multi-color super-resolution by X10 microscopy}},
doi = {10.1016/bs.mcb.2020.04.016},
year = {2020},
}
@article{7949,
abstract = {Peptides derived from non-functional precursors play important roles in various developmental processes, but also in (a)biotic stress signaling. Our (phospho)proteome-wide analyses of C-terminally encoded peptide 5 (CEP5)-mediated changes revealed an impact on abiotic stress-related processes. Drought has a dramatic impact on plant growth, development and reproduction, and the plant hormone auxin plays a role in drought responses. Our genetic, physiological, biochemical and pharmacological results demonstrated that CEP5-mediated signaling is relevant for osmotic and drought stress tolerance in Arabidopsis, and that CEP5 specifically counteracts auxin effects. Specifically, we found that CEP5 signaling stabilizes AUX/IAA transcriptional repressors, suggesting the existence of a novel peptide-dependent control mechanism that tunes auxin signaling. These observations align with the recently described role of AUX/IAAs in stress tolerance and provide a novel role for CEP5 in osmotic and drought stress tolerance.},
author = {Smith, S and Zhu, S and Joos, L and Roberts, I and Nikonorova, N and Vu, LD and Stes, E and Cho, H and Larrieu, A and Xuan, W and Goodall, B and van de Cotte, B and Waite, JM and Rigal, A and R Harborough, SR and Persiau, G and Vanneste, S and Kirschner, GK and Vandermarliere, E and Martens, L and Stahl, Y and Audenaert, D and Friml, Jiří and Felix, G and Simon, R and Bennett, M and Bishopp, A and De Jaeger, G and Ljung, K and Kepinski, S and Robert, S and Nemhauser, J and Hwang, I and Gevaert, K and Beeckman, T and De Smet, I},
issn = {1535-9476},
journal = {Molecular & Cellular Proteomics},
number = {8},
pages = {1248--1262},
publisher = {American Society for Biochemistry and Molecular Biology},
title = {{The CEP5 peptide promotes abiotic stress tolerance, as revealed by quantitative proteomics, and attenuates the AUX/IAA equilibrium in Arabidopsis}},
doi = {10.1074/mcp.ra119.001826},
volume = {19},
year = {2020},
}
@article{6184,
abstract = {We prove edge universality for a general class of correlated real symmetric or complex Hermitian Wigner matrices with arbitrary expectation. Our theorem also applies to internal edges of the self-consistent density of states. In particular, we establish a strong form of band rigidity which excludes mismatches between location and label of eigenvalues close to internal edges in these general models.},
author = {Alt, Johannes and Erdös, László and Krüger, Torben H and Schröder, Dominik J},
journal = {Annals of Probability},
number = {2},
pages = {963--1001},
publisher = {Project Euclid},
title = {{Correlated random matrices: Band rigidity and edge universality}},
volume = {48},
year = {2020},
}
@article{6563,
abstract = {This paper presents two algorithms. The first decides the existence of a pointed homotopy between given simplicial maps 𝑓,𝑔:𝑋→𝑌, and the second computes the group [𝛴𝑋,𝑌]∗ of pointed homotopy classes of maps from a suspension; in both cases, the target Y is assumed simply connected. More generally, these algorithms work relative to 𝐴⊆𝑋.},
author = {Filakovský, Marek and Vokřínek, Lukas},
issn = {16153383},
journal = {Foundations of Computational Mathematics},
pages = {311--330},
publisher = {Springer Nature},
title = {{Are two given maps homotopic? An algorithmic viewpoint}},
doi = {10.1007/s10208-019-09419-x},
volume = {20},
year = {2020},
}
@article{6593,
abstract = {We consider the monotone variational inequality problem in a Hilbert space and describe a projection-type method with inertial terms under the following properties: (a) The method generates a strongly convergent iteration sequence; (b) The method requires, at each iteration, only one projection onto the feasible set and two evaluations of the operator; (c) The method is designed for variational inequality for which the underline operator is monotone and uniformly continuous; (d) The method includes an inertial term. The latter is also shown to speed up the convergence in our numerical results. A comparison with some related methods is given and indicates that the new method is promising.},
author = {Shehu, Yekini and Li, Xiao-Huan and Dong, Qiao-Li},
issn = {1017-1398},
journal = {Numerical Algorithms},
pages = {365--388},
publisher = {Springer Nature},
title = {{An efficient projection-type method for monotone variational inequalities in Hilbert spaces}},
doi = {10.1007/s11075-019-00758-y},
volume = {84},
year = {2020},
}
@article{6649,
abstract = {While Hartree–Fock theory is well established as a fundamental approximation for interacting fermions, it has been unclear how to describe corrections to it due to many-body correlations. In this paper we start from the Hartree–Fock state given by plane waves and introduce collective particle–hole pair excitations. These pairs can be approximately described by a bosonic quadratic Hamiltonian. We use Bogoliubov theory to construct a trial state yielding a rigorous Gell-Mann–Brueckner–type upper bound to the ground state energy. Our result justifies the random-phase approximation in the mean-field scaling regime, for repulsive, regular interaction potentials.
},
author = {Benedikter, Niels P and Nam, Phan Thành and Porta, Marcello and Schlein, Benjamin and Seiringer, Robert},
issn = {1432-0916},
journal = {Communications in Mathematical Physics},
pages = {2097–2150},
publisher = {Springer Nature},
title = {{Optimal upper bound for the correlation energy of a Fermi gas in the mean-field regime}},
doi = {10.1007/s00220-019-03505-5},
volume = {374},
year = {2020},
}
@article{6796,
abstract = {Nearby grid cells have been observed to express a remarkable degree of long-rangeorder, which is often idealized as extending potentially to infinity. Yet their strict peri-odic firing and ensemble coherence are theoretically possible only in flat environments, much unlike the burrows which rodents usually live in. Are the symmetrical, coherent grid maps inferred in the lab relevant to chart their way in their natural habitat? We consider spheres as simple models of curved environments and waiting for the appropriate experiments to be performed, we use our adaptation model to predict what grid maps would emerge in a network with the same type of recurrent connections, which on the plane produce coherence among the units. We find that on the sphere such connections distort the maps that single grid units would express on their own, and aggregate them into clusters. When remapping to a different spherical environment, units in each cluster maintain only partial coherence, similar to what is observed in disordered materials, such as spin glasses.},
author = {Stella, Federico and Urdapilleta, Eugenio and Luo, Yifan and Treves, Alessandro},
issn = {10981063},
journal = {Hippocampus},
number = {4},
pages = {302--313},
publisher = {Wiley},
title = {{Partial coherence and frustration in self-organizing spherical grids}},
doi = {10.1002/hipo.23144},
volume = {30},
year = {2020},
}
@article{6808,
abstract = {Super-resolution fluorescence microscopy has become an important catalyst for discovery in the life sciences. In STimulated Emission Depletion (STED) microscopy, a pattern of light drives fluorophores from a signal-emitting on-state to a non-signalling off-state. Only emitters residing in a sub-diffraction volume around an intensity minimum are allowed to fluoresce, rendering them distinguishable from the nearby, but dark fluorophores. STED routinely achieves resolution in the few tens of nanometers range in biological samples and is suitable for live imaging. Here, we review the working principle of STED and provide general guidelines for successful STED imaging. The strive for ever higher resolution comes at the cost of increased light burden. We discuss techniques to reduce light exposure and mitigate its detrimental effects on the specimen. These include specialized illumination strategies as well as protecting fluorophores from photobleaching mediated by high-intensity STED light. This opens up the prospect of volumetric imaging in living cells and tissues with diffraction-unlimited resolution in all three spatial dimensions.},
author = {Jahr, Wiebke and Velicky, Philipp and Danzl, Johann G},
issn = {1046-2023},
journal = {Methods},
number = {3},
pages = {27--41},
publisher = {Elsevier},
title = {{Strategies to maximize performance in STimulated Emission Depletion (STED) nanoscopy of biological specimens}},
doi = {10.1016/j.ymeth.2019.07.019},
volume = {174},
year = {2020},
}
@article{7956,
abstract = {When short-range attractions are combined with long-range repulsions in colloidal particle systems, complex microphases can emerge. Here, we study a system of isotropic particles, which can form lamellar structures or a disordered fluid phase when temperature is varied. We show that, at equilibrium, the lamellar structure crystallizes, while out of equilibrium, the system forms a variety of structures at different shear rates and temperatures above melting. The shear-induced ordering is analyzed by means of principal component analysis and artificial neural networks, which are applied to data of reduced dimensionality. Our results reveal the possibility of inducing ordering by shear, potentially providing a feasible route to the fabrication of ordered lamellar structures from isotropic particles.},
author = {Pȩkalski, J. and Rzadkowski, Wojciech and Panagiotopoulos, A. Z.},
issn = {10897690},
journal = {The Journal of chemical physics},
number = {20},
publisher = {AIP},
title = {{Shear-induced ordering in systems with competing interactions: A machine learning study}},
doi = {10.1063/5.0005194},
volume = {152},
year = {2020},
}
@article{7962,
abstract = {A string graph is the intersection graph of a family of continuous arcs in the plane. The intersection graph of a family of plane convex sets is a string graph, but not all string graphs can be obtained in this way. We prove the following structure theorem conjectured by Janson and Uzzell: The vertex set of almost all string graphs on n vertices can be partitioned into five cliques such that some pair of them is not connected by any edge (n→∞). We also show that every graph with the above property is an intersection graph of plane convex sets. As a corollary, we obtain that almost all string graphs on n vertices are intersection graphs of plane convex sets.},
author = {Pach, János and Reed, Bruce and Yuditsky, Yelena},
issn = {14320444},
journal = {Discrete and Computational Geometry},
number = {4},
pages = {888--917},
publisher = {Springer Nature},
title = {{Almost all string graphs are intersection graphs of plane convex sets}},
doi = {10.1007/s00454-020-00213-z},
volume = {63},
year = {2020},
}
@inproceedings{7966,
abstract = {For 1≤m≤n, we consider a natural m-out-of-n multi-instance scenario for a public-key encryption (PKE) scheme. An adversary, given n independent instances of PKE, wins if he breaks at least m out of the n instances. In this work, we are interested in the scaling factor of PKE schemes, SF, which measures how well the difficulty of breaking m out of the n instances scales in m. That is, a scaling factor SF=ℓ indicates that breaking m out of n instances is at least ℓ times more difficult than breaking one single instance. A PKE scheme with small scaling factor hence provides an ideal target for mass surveillance. In fact, the Logjam attack (CCS 2015) implicitly exploited, among other things, an almost constant scaling factor of ElGamal over finite fields (with shared group parameters).
For Hashed ElGamal over elliptic curves, we use the generic group model to argue that the scaling factor depends on the scheme's granularity. In low granularity, meaning each public key contains its independent group parameter, the scheme has optimal scaling factor SF=m; In medium and high granularity, meaning all public keys share the same group parameter, the scheme still has a reasonable scaling factor SF=√m. Our findings underline that instantiating ElGamal over elliptic curves should be preferred to finite fields in a multi-instance scenario.
As our main technical contribution, we derive new generic-group lower bounds of Ω(√(mp)) on the difficulty of solving both the m-out-of-n Gap Discrete Logarithm and the m-out-of-n Gap Computational Diffie-Hellman problem over groups of prime order p, extending a recent result by Yun (EUROCRYPT 2015). We establish the lower bound by studying the hardness of a related computational problem which we call the search-by-hypersurface problem.},
author = {Auerbach, Benedikt and Giacon, Federico and Kiltz, Eike},
booktitle = {Advances in Cryptology – EUROCRYPT 2020},
isbn = {9783030457266},
issn = {0302-9743},
pages = {475--506},
publisher = {Springer Nature},
title = {{Everybody’s a target: Scalability in public-key encryption}},
doi = {10.1007/978-3-030-45727-3_16},
volume = {12107},
year = {2020},
}