---
_id: '15016'
abstract:
- lang: eng
text: 'The development, evolution, and function of the vertebrate central nervous
system (CNS) can be best studied using diverse model organisms. Amphibians, with
their unique phylogenetic position at the transition between aquatic and terrestrial
lifestyles, are valuable for understanding the origin and evolution of the tetrapod
brain and spinal cord. Their metamorphic developmental transitions and unique
regenerative abilities also facilitate the discovery of mechanisms for neural
circuit remodeling and replacement. The genetic toolkit for amphibians, however,
remains limited, with only a few species having sequenced genomes and a small
number of transgenic lines available. In mammals, recombinant adeno-associated
viral vectors (AAVs) have become a powerful alternative to genome modification
for visualizing and perturbing the nervous system. AAVs are DNA viruses that enable
neuronal transduction in both developing and adult animals with low toxicity and
spatial, temporal, and cell-type specificity. However, AAVs have never been shown
to transduce amphibian cells efficiently. To bridge this gap, we established a
simple, scalable, and robust strategy to screen AAV serotypes in three distantly-related
amphibian species: the frogs Xenopus laevis and Pelophylax bedriagae, and the
salamander Pleurodeles waltl, in both developing larval tadpoles and post-metamorphic
animals. For each species, we successfully identified at least two AAV serotypes
capable of infecting the CNS; however, no pan-amphibian serotype was identified,
indicating rapid evolution of AAV tropism. In addition, we developed an AAV-based
strategy that targets isochronic cohorts of developing neurons – a critical tool
for parsing neural circuit assembly. Finally, to enable visualization and manipulation
of neural circuits, we identified AAV variants for retrograde tracing of neuronal
projections in adult animals. Our findings expand the toolkit for amphibians to
include AAVs, establish a generalizable workflow for AAV screening in non-canonical
research organisms, generate testable hypotheses for the evolution of AAV tropism,
and lay the foundation for modern cross-species comparisons of vertebrate CNS
development, function, and evolution. '
acknowledgement: "We would like to extend our thanks to members of the Sweeney, Tosches,
Shein-Idelson,\r\nYamaguchi, Kelley, and Cline Labs for their contributions to this
project, discussion and support.\r\nWe additionally thank the Beckman Institute
Clover Center and Viviana Gradinaru (Caltech),\r\nKimberly Ritola (UNC NeuroTools),
Flavia Gama Gomez Leite (ISTA Viral Core), and Hüseyin\r\nCihan Önal (Shigemoto
Group, ISTA) for their consultation and assistance regarding AAVs, as\r\nwell as
Andras Simon and Alberto Joven for feedback and discussions on AAVs in Pleurodeles.\r\nTo
do these experiments, we have also benefited from the tremendous support of our
animal care and imaging facilities at our respective institutions, as well as the
amphibian stock centers\r\n(National Xenopus Resource Center, European Xenopus Resource
Center, Xenopus Express)\r\nand our funding sources: U.S. National Science Foundation
(NSF) Grant Number IOS 2110086\r\n(D.B.K., L.B.S., M.A.T., A.Y., and H.T.C.); United
States-Israel Binational Science Foundation\r\n(BSF) Grant Number 2020702 (M.S.-I.);
NSF Award Number 1645105 (G.J.G., M.E.H.); FTI\r\nStrategy Lower Austria Dissertation
Grant Number FTI21-D-046 (D.V.); Horizon Europe ERC\r\nStarting Grant Number 101041551
(L.B.S.); NIH grant number R35GM146973 (M.A.T.); Rita Allen\r\nFoundation award
number GA_032522_FE (M.A.T.); European Molecular Biology Organization\r\nLong-Term
Fellowship ALTF 874-2021 (A.D.); National Science Foundation Graduate Research\r\nFellowship
DGE 2036197 (E.C.J.B.); NIH grant number P40OD010997 (M.E.H)."
article_processing_charge: No
author:
- first_name: Eliza C.B.
full_name: Jaeger, Eliza C.B.
last_name: Jaeger
- first_name: David
full_name: Vijatovic, David
id: cf391e77-ec3c-11ea-a124-d69323410b58
last_name: Vijatovic
- first_name: Astrid
full_name: Deryckere, Astrid
last_name: Deryckere
- first_name: Nikol
full_name: Zorin, Nikol
last_name: Zorin
- first_name: Akemi L.
full_name: Nguyen, Akemi L.
last_name: Nguyen
- first_name: Georgiy
full_name: Ivanian, Georgiy
id: eaf2b366-cfd1-11ee-bbdf-c8790f800a05
last_name: Ivanian
- first_name: Jamie
full_name: Woych, Jamie
last_name: Woych
- first_name: Rebecca C
full_name: Arnold, Rebecca C
id: d6cce458-14c9-11ed-a755-c1c8fc6fde6f
last_name: Arnold
- first_name: Alonso
full_name: Ortega Gurrola, Alonso
last_name: Ortega Gurrola
- first_name: Arik
full_name: Shvartsman, Arik
last_name: Shvartsman
- first_name: Francesca
full_name: Barbieri, Francesca
id: a9492887-8972-11ed-ae7b-bfae10998254
last_name: Barbieri
- first_name: Florina-Alexandra
full_name: Toma, Florina-Alexandra
id: 85dd99f2-15b2-11ec-abd3-d1ae4d57f3b5
last_name: Toma
- first_name: Gary J.
full_name: Gorbsky, Gary J.
last_name: Gorbsky
- first_name: Marko E.
full_name: Horb, Marko E.
last_name: Horb
- first_name: Hollis T.
full_name: Cline, Hollis T.
last_name: Cline
- first_name: Timothy F.
full_name: Shay, Timothy F.
last_name: Shay
- first_name: Darcy B.
full_name: Kelley, Darcy B.
last_name: Kelley
- first_name: Ayako
full_name: Yamaguchi, Ayako
last_name: Yamaguchi
- first_name: Mark
full_name: Shein-Idelson, Mark
last_name: Shein-Idelson
- first_name: Maria Antonietta
full_name: Tosches, Maria Antonietta
last_name: Tosches
- first_name: Lora Beatrice Jaeger
full_name: Sweeney, Lora Beatrice Jaeger
id: 56BE8254-C4F0-11E9-8E45-0B23E6697425
last_name: Sweeney
orcid: 0000-0001-9242-5601
citation:
ama: Jaeger ECB, Vijatovic D, Deryckere A, et al. Adeno-associated viral tools to
trace neural development and connectivity across amphibians. bioRxiv. doi:10.1101/2024.02.15.580289
apa: Jaeger, E. C. B., Vijatovic, D., Deryckere, A., Zorin, N., Nguyen, A. L., Ivanian,
G., … Sweeney, L. B. (n.d.). Adeno-associated viral tools to trace neural development
and connectivity across amphibians. bioRxiv. https://doi.org/10.1101/2024.02.15.580289
chicago: Jaeger, Eliza C.B., David Vijatovic, Astrid Deryckere, Nikol Zorin, Akemi
L. Nguyen, Georgiy Ivanian, Jamie Woych, et al. “Adeno-Associated Viral Tools
to Trace Neural Development and Connectivity across Amphibians.” BioRxiv,
n.d. https://doi.org/10.1101/2024.02.15.580289.
ieee: E. C. B. Jaeger et al., “Adeno-associated viral tools to trace neural
development and connectivity across amphibians,” bioRxiv. .
ista: Jaeger ECB, Vijatovic D, Deryckere A, Zorin N, Nguyen AL, Ivanian G, Woych
J, Arnold RC, Ortega Gurrola A, Shvartsman A, Barbieri F, Toma F-A, Gorbsky GJ,
Horb ME, Cline HT, Shay TF, Kelley DB, Yamaguchi A, Shein-Idelson M, Tosches MA,
Sweeney LB. Adeno-associated viral tools to trace neural development and connectivity
across amphibians. bioRxiv, 10.1101/2024.02.15.580289.
mla: Jaeger, Eliza C. B., et al. “Adeno-Associated Viral Tools to Trace Neural Development
and Connectivity across Amphibians.” BioRxiv, doi:10.1101/2024.02.15.580289.
short: E.C.B. Jaeger, D. Vijatovic, A. Deryckere, N. Zorin, A.L. Nguyen, G. Ivanian,
J. Woych, R.C. Arnold, A. Ortega Gurrola, A. Shvartsman, F. Barbieri, F.-A. Toma,
G.J. Gorbsky, M.E. Horb, H.T. Cline, T.F. Shay, D.B. Kelley, A. Yamaguchi, M.
Shein-Idelson, M.A. Tosches, L.B. Sweeney, BioRxiv (n.d.).
date_created: 2024-02-20T09:20:32Z
date_published: 2024-02-16T00:00:00Z
date_updated: 2024-02-20T09:34:25Z
day: '16'
department:
- _id: LoSw
- _id: MaDe
- _id: GaNo
doi: 10.1101/2024.02.15.580289
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2024.02.15.580289
month: '02'
oa: 1
oa_version: Preprint
project:
- _id: bd73af52-d553-11ed-ba76-912049f0ac7a
grant_number: FTI21-D-046
name: Entwicklung und Funktion der V1 Interneuronen vom Schwimmen zum Laufen während
der Metamorphose von Xenopus
- _id: ebb66355-77a9-11ec-83b8-b8ac210a4dae
grant_number: '101041551'
name: Development and Evolution of Tetrapod Motor Circuits
publication: bioRxiv
publication_status: submitted
status: public
title: Adeno-associated viral tools to trace neural development and connectivity across
amphibians
type: preprint
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2024'
...
---
_id: '14443'
abstract:
- lang: eng
text: "Importance Climate change, pollution, urbanization, socioeconomic inequality,
and psychosocial effects of the COVID-19 pandemic have caused massive changes
in environmental conditions that affect brain health during the life span, both
on a population level as well as on the level of the individual. How these environmental
factors influence the brain, behavior, and mental illness is not well known.\r\nObservations
\ A research strategy enabling population neuroscience to contribute to identify
brain mechanisms underlying environment-related mental illness by leveraging innovative
enrichment tools for data federation, geospatial observation, climate and pollution
measures, digital health, and novel data integration techniques is described.
This strategy can inform innovative treatments that target causal cognitive and
molecular mechanisms of mental illness related to the environment. An example
is presented of the environMENTAL Project that is leveraging federated cohort
data of over 1.5 million European citizens and patients enriched with deep phenotyping
data from large-scale behavioral neuroimaging cohorts to identify brain mechanisms
related to environmental adversity underlying symptoms of depression, anxiety,
stress, and substance misuse.\r\nConclusions and Relevance This research will
lead to the development of objective biomarkers and evidence-based interventions
that will significantly improve outcomes of environment-related mental illness."
article_processing_charge: No
article_type: review
author:
- first_name: Gunter
full_name: Schumann, Gunter
last_name: Schumann
- first_name: Ole A.
full_name: Andreassen, Ole A.
last_name: Andreassen
- first_name: Tobias
full_name: Banaschewski, Tobias
last_name: Banaschewski
- first_name: Vince D.
full_name: Calhoun, Vince D.
last_name: Calhoun
- first_name: Nicholas
full_name: Clinton, Nicholas
last_name: Clinton
- first_name: Sylvane
full_name: Desrivieres, Sylvane
last_name: Desrivieres
- first_name: Ragnhild Eek
full_name: Brandlistuen, Ragnhild Eek
last_name: Brandlistuen
- first_name: Jianfeng
full_name: Feng, Jianfeng
last_name: Feng
- first_name: Soeren
full_name: Hese, Soeren
last_name: Hese
- first_name: Esther
full_name: Hitchen, Esther
last_name: Hitchen
- first_name: Per
full_name: Hoffmann, Per
last_name: Hoffmann
- first_name: Tianye
full_name: Jia, Tianye
last_name: Jia
- first_name: Viktor
full_name: Jirsa, Viktor
last_name: Jirsa
- first_name: Andre F.
full_name: Marquand, Andre F.
last_name: Marquand
- first_name: Frauke
full_name: Nees, Frauke
last_name: Nees
- first_name: Markus M.
full_name: Nöthen, Markus M.
last_name: Nöthen
- first_name: Gaia
full_name: Novarino, Gaia
id: 3E57A680-F248-11E8-B48F-1D18A9856A87
last_name: Novarino
orcid: 0000-0002-7673-7178
- first_name: Elli
full_name: Polemiti, Elli
last_name: Polemiti
- first_name: Markus
full_name: Ralser, Markus
last_name: Ralser
- first_name: Michael
full_name: Rapp, Michael
last_name: Rapp
- first_name: Kerstin
full_name: Schepanski, Kerstin
last_name: Schepanski
- first_name: Tamara
full_name: Schikowski, Tamara
last_name: Schikowski
- first_name: Mel
full_name: Slater, Mel
last_name: Slater
- first_name: Peter
full_name: Sommer, Peter
last_name: Sommer
- first_name: Bernd Carsten
full_name: Stahl, Bernd Carsten
last_name: Stahl
- first_name: Paul M.
full_name: Thompson, Paul M.
last_name: Thompson
- first_name: Sven
full_name: Twardziok, Sven
last_name: Twardziok
- first_name: Dennis
full_name: Van Der Meer, Dennis
last_name: Van Der Meer
- first_name: Henrik
full_name: Walter, Henrik
last_name: Walter
- first_name: Lars
full_name: Westlye, Lars
last_name: Westlye
citation:
ama: 'Schumann G, Andreassen OA, Banaschewski T, et al. Addressing global environmental
challenges to mental health using population neuroscience: A review. JAMA Psychiatry.
2023;80(10):1066-1074. doi:10.1001/jamapsychiatry.2023.2996'
apa: 'Schumann, G., Andreassen, O. A., Banaschewski, T., Calhoun, V. D., Clinton,
N., Desrivieres, S., … Westlye, L. (2023). Addressing global environmental challenges
to mental health using population neuroscience: A review. JAMA Psychiatry.
American Medical Association. https://doi.org/10.1001/jamapsychiatry.2023.2996'
chicago: 'Schumann, Gunter, Ole A. Andreassen, Tobias Banaschewski, Vince D. Calhoun,
Nicholas Clinton, Sylvane Desrivieres, Ragnhild Eek Brandlistuen, et al. “Addressing
Global Environmental Challenges to Mental Health Using Population Neuroscience:
A Review.” JAMA Psychiatry. American Medical Association, 2023. https://doi.org/10.1001/jamapsychiatry.2023.2996.'
ieee: 'G. Schumann et al., “Addressing global environmental challenges to
mental health using population neuroscience: A review,” JAMA Psychiatry,
vol. 80, no. 10. American Medical Association, pp. 1066–1074, 2023.'
ista: 'Schumann G, Andreassen OA, Banaschewski T, Calhoun VD, Clinton N, Desrivieres
S, Brandlistuen RE, Feng J, Hese S, Hitchen E, Hoffmann P, Jia T, Jirsa V, Marquand
AF, Nees F, Nöthen MM, Novarino G, Polemiti E, Ralser M, Rapp M, Schepanski K,
Schikowski T, Slater M, Sommer P, Stahl BC, Thompson PM, Twardziok S, Van Der
Meer D, Walter H, Westlye L. 2023. Addressing global environmental challenges
to mental health using population neuroscience: A review. JAMA Psychiatry. 80(10),
1066–1074.'
mla: 'Schumann, Gunter, et al. “Addressing Global Environmental Challenges to Mental
Health Using Population Neuroscience: A Review.” JAMA Psychiatry, vol.
80, no. 10, American Medical Association, 2023, pp. 1066–74, doi:10.1001/jamapsychiatry.2023.2996.'
short: G. Schumann, O.A. Andreassen, T. Banaschewski, V.D. Calhoun, N. Clinton,
S. Desrivieres, R.E. Brandlistuen, J. Feng, S. Hese, E. Hitchen, P. Hoffmann,
T. Jia, V. Jirsa, A.F. Marquand, F. Nees, M.M. Nöthen, G. Novarino, E. Polemiti,
M. Ralser, M. Rapp, K. Schepanski, T. Schikowski, M. Slater, P. Sommer, B.C. Stahl,
P.M. Thompson, S. Twardziok, D. Van Der Meer, H. Walter, L. Westlye, JAMA Psychiatry
80 (2023) 1066–1074.
date_created: 2023-10-22T22:01:14Z
date_published: 2023-10-01T00:00:00Z
date_updated: 2023-10-31T12:17:20Z
day: '01'
department:
- _id: GaNo
doi: 10.1001/jamapsychiatry.2023.2996
external_id:
pmid:
- '37610741'
intvolume: ' 80'
issue: '10'
language:
- iso: eng
month: '10'
oa_version: None
page: 1066-1074
pmid: 1
publication: JAMA Psychiatry
publication_identifier:
eissn:
- 2168-6238
publication_status: published
publisher: American Medical Association
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Addressing global environmental challenges to mental health using population
neuroscience: A review'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 80
year: '2023'
...
---
_id: '13168'
abstract:
- lang: eng
text: Urban-living individuals are exposed to many environmental factors that may
combine and interact to influence mental health. While individual factors of an
urban environment have been investigated in isolation, no attempt has been made
to model how complex, real-life exposure to living in the city relates to brain
and mental health, and how this is moderated by genetic factors. Using the data
of 156,075 participants from the UK Biobank, we carried out sparse canonical correlation
analyses to investigate the relationships between urban environments and psychiatric
symptoms. We found an environmental profile of social deprivation, air pollution,
street network and urban land-use density that was positively correlated with
an affective symptom group (r = 0.22, Pperm < 0.001), mediated by brain volume
differences consistent with reward processing, and moderated by genes enriched
for stress response, including CRHR1, explaining 2.01% of the variance in brain
volume differences. Protective factors such as greenness and generous destination
accessibility were negatively correlated with an anxiety symptom group (r = 0.10,
Pperm < 0.001), mediated by brain regions necessary for emotion regulation and
moderated by EXD3, explaining 1.65% of the variance. The third urban environmental
profile was correlated with an emotional instability symptom group (r = 0.03,
Pperm < 0.001). Our findings suggest that different environmental profiles of
urban living may influence specific psychiatric symptom groups through distinct
neurobiological pathways.
acknowledgement: This work received support from the European Union-funded Horizon
Europe project ‘environMENTAL’ (no. 101057429 to G.S., A.M. and M.M.N.) and cofunding
by UK Research and Innovation under the UK Government’s Horizon Europe funding guarantee
(nos. 10041392 and 10038599) for study design and data analysis; the Horizon 2020-funded
European Research Council Advanced Grant ‘STRATIFY’ (no. 695313 to G.S. for study
design and data analysis); the Human Brain Project (HBP SGA3, no. 945539 to G.S.
for study design and data analysis); the National Institutes of Health (grant no.
R01DA049238 to G.S. for study design and data analysis); the German Research Foundation
(COPE; grant no. 675346 to G.S. for study design and data analysis); the National
Natural Science Foundation of China (grant no. 82001797 to J.X., grant no. 82030053
to C.Y., grant no. 82202093 to J.T. and grant no. 82150710554 to G.S. for study
design, data analysis and preparation of the manuscript); National Key Research
and Development Program of China (grant no. 2018YFC1314301 to C.Y. for study design
and data analysis); Tianjin Applied Basic Research Diversified Investment Foundation
(grant no. 21JCYBJC01360 to J.X. for study design and data analysis); Tianjin Health
Technology Project (grant no. TJWJ2021QN002 to J.X. for preparation of the manuscript);
Science & Technology Development Fund of the Tianjin Education Commission for Higher
Education (grant no. 2019KJ195 to J.X. for preparation of the manuscript); the Tianjin
Medical University ‘Clinical Talent Training 123 Climbing Plan’ to J.X. for the
preparation of the manuscript; Tianjin Key Medical Discipline (Specialty) Construction
Project (grant no. TJYXZDXK-001A to C.Y. for preparation of the manuscript); the
National Key R&D Program of China (grant no. 2022YFE0209400 to L.Y. for study design
and data analysis); the Tsinghua University Initiative Scientific Research Program
(grant no. 2021Z11GHX002 to L.Y. for study design and data analysis); the National
Key Scientific and Technological Infrastructure Project ‘Earth System Science Numerical
Simulator Facility’ (EarthLab to L.Y. for study design and data analysis); the Chinese
National High-end Foreign Expert Recruitment Plan to G.S.; and the Alexander von
Humboldt Foundation to G.S. for study design and data analysis.
article_processing_charge: No
article_type: original
author:
- first_name: Jiayuan
full_name: Xu, Jiayuan
last_name: Xu
- first_name: Nana
full_name: Liu, Nana
last_name: Liu
- first_name: Elli
full_name: Polemiti, Elli
last_name: Polemiti
- first_name: Liliana
full_name: Garcia-Mondragon, Liliana
last_name: Garcia-Mondragon
- first_name: Jie
full_name: Tang, Jie
last_name: Tang
- first_name: Xiaoxuan
full_name: Liu, Xiaoxuan
last_name: Liu
- first_name: Tristram
full_name: Lett, Tristram
last_name: Lett
- first_name: Le
full_name: Yu, Le
last_name: Yu
- first_name: Markus M.
full_name: Nöthen, Markus M.
last_name: Nöthen
- first_name: Jianfeng
full_name: Feng, Jianfeng
last_name: Feng
- first_name: Chunshui
full_name: Yu, Chunshui
last_name: Yu
- first_name: Andre
full_name: Marquand, Andre
last_name: Marquand
- first_name: Gunter
full_name: Schumann, Gunter
last_name: Schumann
- first_name: Henrik
full_name: Walter, Henrik
last_name: Walter
- first_name: Andreas
full_name: Heinz, Andreas
last_name: Heinz
- first_name: Markus
full_name: Ralser, Markus
last_name: Ralser
- first_name: Sven
full_name: Twardziok, Sven
last_name: Twardziok
- first_name: Nilakshi
full_name: Vaidya, Nilakshi
last_name: Vaidya
- first_name: Emin
full_name: Serin, Emin
last_name: Serin
- first_name: Marcel
full_name: Jentsch, Marcel
last_name: Jentsch
- first_name: Esther
full_name: Hitchen, Esther
last_name: Hitchen
- first_name: Roland
full_name: Eils, Roland
last_name: Eils
- first_name: Ulrike Helene
full_name: Taron, Ulrike Helene
last_name: Taron
- first_name: Tatjana
full_name: Schütz, Tatjana
last_name: Schütz
- first_name: Kerstin
full_name: Schepanski, Kerstin
last_name: Schepanski
- first_name: Jamie
full_name: Banks, Jamie
last_name: Banks
- first_name: Tobias
full_name: Banaschewski, Tobias
last_name: Banaschewski
- first_name: Karina
full_name: Jansone, Karina
last_name: Jansone
- first_name: Nina
full_name: Christmann, Nina
last_name: Christmann
- first_name: Andreas
full_name: Meyer-Lindenberg, Andreas
last_name: Meyer-Lindenberg
- first_name: Heike
full_name: Tost, Heike
last_name: Tost
- first_name: Nathalie
full_name: Holz, Nathalie
last_name: Holz
- first_name: Emanuel
full_name: Schwarz, Emanuel
last_name: Schwarz
- first_name: Argyris
full_name: Stringaris, Argyris
last_name: Stringaris
- first_name: Maja
full_name: Neidhart, Maja
last_name: Neidhart
- first_name: Frauke
full_name: Nees, Frauke
last_name: Nees
- first_name: Sebastian
full_name: Siehl, Sebastian
last_name: Siehl
- first_name: Ole
full_name: A. Andreassen, Ole
last_name: A. Andreassen
- first_name: Lars
full_name: T. Westlye, Lars
last_name: T. Westlye
- first_name: Dennis
full_name: Van Der Meer, Dennis
last_name: Van Der Meer
- first_name: Sara
full_name: Fernandez, Sara
last_name: Fernandez
- first_name: Rikka
full_name: Kjelkenes, Rikka
last_name: Kjelkenes
- first_name: Helga
full_name: Ask, Helga
last_name: Ask
- first_name: Michael
full_name: Rapp, Michael
last_name: Rapp
- first_name: Mira
full_name: Tschorn, Mira
last_name: Tschorn
- first_name: Sarah Jane
full_name: Böttger, Sarah Jane
last_name: Böttger
- first_name: Gaia
full_name: Novarino, Gaia
id: 3E57A680-F248-11E8-B48F-1D18A9856A87
last_name: Novarino
orcid: 0000-0002-7673-7178
- first_name: Lena
full_name: Marr, Lena
id: 4406F586-F248-11E8-B48F-1D18A9856A87
last_name: Marr
- first_name: Mel
full_name: Slater, Mel
last_name: Slater
- first_name: Guillem Feixas
full_name: Viapiana, Guillem Feixas
last_name: Viapiana
- first_name: Francisco Eiroa
full_name: Orosa, Francisco Eiroa
last_name: Orosa
- first_name: Jaime
full_name: Gallego, Jaime
last_name: Gallego
- first_name: Alvaro
full_name: Pastor, Alvaro
last_name: Pastor
- first_name: Andreas
full_name: Forstner, Andreas
last_name: Forstner
- first_name: Per
full_name: Hoffmann, Per
last_name: Hoffmann
- first_name: Markus
full_name: M. Nöthen, Markus
last_name: M. Nöthen
- first_name: Andreas
full_name: J. Forstner, Andreas
last_name: J. Forstner
- first_name: Isabelle
full_name: Claus, Isabelle
last_name: Claus
- first_name: Abbi
full_name: Miller, Abbi
last_name: Miller
- first_name: Stefanie
full_name: Heilmann-Heimbach, Stefanie
last_name: Heilmann-Heimbach
- first_name: Peter
full_name: Sommer, Peter
last_name: Sommer
- first_name: Mona
full_name: Boye, Mona
last_name: Boye
- first_name: Johannes
full_name: Wilbertz, Johannes
last_name: Wilbertz
- first_name: Karen
full_name: Schmitt, Karen
last_name: Schmitt
- first_name: Viktor
full_name: Jirsa, Viktor
last_name: Jirsa
- first_name: Spase
full_name: Petkoski, Spase
last_name: Petkoski
- first_name: Séverine
full_name: Pitel, Séverine
last_name: Pitel
- first_name: Lisa
full_name: Otten, Lisa
last_name: Otten
- first_name: Anastasios Polykarpos
full_name: Athanasiadis, Anastasios Polykarpos
last_name: Athanasiadis
- first_name: Charlie
full_name: Pearmund, Charlie
last_name: Pearmund
- first_name: Bernhard
full_name: Spanlang, Bernhard
last_name: Spanlang
- first_name: Elena
full_name: Alvarez, Elena
last_name: Alvarez
- first_name: Mavi
full_name: Sanchez, Mavi
last_name: Sanchez
- first_name: Arantxa
full_name: Giner, Arantxa
last_name: Giner
- first_name: Sören
full_name: Hese, Sören
last_name: Hese
- first_name: Paul
full_name: Renner, Paul
last_name: Renner
- first_name: Tianye
full_name: Jia, Tianye
last_name: Jia
- first_name: Yanting
full_name: Gong, Yanting
last_name: Gong
- first_name: Yunman
full_name: Xia, Yunman
last_name: Xia
- first_name: Xiao
full_name: Chang, Xiao
last_name: Chang
- first_name: Vince
full_name: Calhoun, Vince
last_name: Calhoun
- first_name: Jingyu
full_name: Liu, Jingyu
last_name: Liu
- first_name: Paul
full_name: Thompson, Paul
last_name: Thompson
- first_name: Nicholas
full_name: Clinton, Nicholas
last_name: Clinton
- first_name: Sylvane
full_name: Desrivieres, Sylvane
last_name: Desrivieres
- first_name: Allan
full_name: H. Young, Allan
last_name: H. Young
- first_name: Bernd
full_name: Stahl, Bernd
last_name: Stahl
- first_name: George
full_name: Ogoh, George
last_name: Ogoh
citation:
ama: Xu J, Liu N, Polemiti E, et al. Effects of urban living environments on mental
health in adults. Nature Medicine. 2023;29:1456-1467. doi:10.1038/s41591-023-02365-w
apa: Xu, J., Liu, N., Polemiti, E., Garcia-Mondragon, L., Tang, J., Liu, X., … Ogoh,
G. (2023). Effects of urban living environments on mental health in adults. Nature
Medicine. Springer Nature. https://doi.org/10.1038/s41591-023-02365-w
chicago: Xu, Jiayuan, Nana Liu, Elli Polemiti, Liliana Garcia-Mondragon, Jie Tang,
Xiaoxuan Liu, Tristram Lett, et al. “Effects of Urban Living Environments on Mental
Health in Adults.” Nature Medicine. Springer Nature, 2023. https://doi.org/10.1038/s41591-023-02365-w.
ieee: J. Xu et al., “Effects of urban living environments on mental health
in adults,” Nature Medicine, vol. 29. Springer Nature, pp. 1456–1467, 2023.
ista: Xu J, Liu N, Polemiti E, Garcia-Mondragon L, Tang J, Liu X, Lett T, Yu L,
Nöthen MM, Feng J, Yu C, Marquand A, Schumann G, Walter H, Heinz A, Ralser M,
Twardziok S, Vaidya N, Serin E, Jentsch M, Hitchen E, Eils R, Taron UH, Schütz
T, Schepanski K, Banks J, Banaschewski T, Jansone K, Christmann N, Meyer-Lindenberg
A, Tost H, Holz N, Schwarz E, Stringaris A, Neidhart M, Nees F, Siehl S, A. Andreassen
O, T. Westlye L, Van Der Meer D, Fernandez S, Kjelkenes R, Ask H, Rapp M, Tschorn
M, Böttger SJ, Novarino G, Marr L, Slater M, Viapiana GF, Orosa FE, Gallego J,
Pastor A, Forstner A, Hoffmann P, M. Nöthen M, J. Forstner A, Claus I, Miller
A, Heilmann-Heimbach S, Sommer P, Boye M, Wilbertz J, Schmitt K, Jirsa V, Petkoski
S, Pitel S, Otten L, Athanasiadis AP, Pearmund C, Spanlang B, Alvarez E, Sanchez
M, Giner A, Hese S, Renner P, Jia T, Gong Y, Xia Y, Chang X, Calhoun V, Liu J,
Thompson P, Clinton N, Desrivieres S, H. Young A, Stahl B, Ogoh G. 2023. Effects
of urban living environments on mental health in adults. Nature Medicine. 29,
1456–1467.
mla: Xu, Jiayuan, et al. “Effects of Urban Living Environments on Mental Health
in Adults.” Nature Medicine, vol. 29, Springer Nature, 2023, pp. 1456–67,
doi:10.1038/s41591-023-02365-w.
short: J. Xu, N. Liu, E. Polemiti, L. Garcia-Mondragon, J. Tang, X. Liu, T. Lett,
L. Yu, M.M. Nöthen, J. Feng, C. Yu, A. Marquand, G. Schumann, H. Walter, A. Heinz,
M. Ralser, S. Twardziok, N. Vaidya, E. Serin, M. Jentsch, E. Hitchen, R. Eils,
U.H. Taron, T. Schütz, K. Schepanski, J. Banks, T. Banaschewski, K. Jansone, N.
Christmann, A. Meyer-Lindenberg, H. Tost, N. Holz, E. Schwarz, A. Stringaris,
M. Neidhart, F. Nees, S. Siehl, O. A. Andreassen, L. T. Westlye, D. Van Der Meer,
S. Fernandez, R. Kjelkenes, H. Ask, M. Rapp, M. Tschorn, S.J. Böttger, G. Novarino,
L. Marr, M. Slater, G.F. Viapiana, F.E. Orosa, J. Gallego, A. Pastor, A. Forstner,
P. Hoffmann, M. M. Nöthen, A. J. Forstner, I. Claus, A. Miller, S. Heilmann-Heimbach,
P. Sommer, M. Boye, J. Wilbertz, K. Schmitt, V. Jirsa, S. Petkoski, S. Pitel,
L. Otten, A.P. Athanasiadis, C. Pearmund, B. Spanlang, E. Alvarez, M. Sanchez,
A. Giner, S. Hese, P. Renner, T. Jia, Y. Gong, Y. Xia, X. Chang, V. Calhoun, J.
Liu, P. Thompson, N. Clinton, S. Desrivieres, A. H. Young, B. Stahl, G. Ogoh,
Nature Medicine 29 (2023) 1456–1467.
date_created: 2023-06-25T22:00:46Z
date_published: 2023-06-15T00:00:00Z
date_updated: 2023-12-13T11:25:55Z
day: '15'
ddc:
- '570'
department:
- _id: GaNo
doi: 10.1038/s41591-023-02365-w
external_id:
isi:
- '001013172700001'
file:
- access_level: open_access
checksum: bcd3225b2731c3442fa98987fd3bd46d
content_type: application/pdf
creator: dernst
date_created: 2023-06-26T10:15:44Z
date_updated: 2023-06-26T10:15:44Z
file_id: '13171'
file_name: 2023_NatureMedicine_Xu.pdf
file_size: 7365360
relation: main_file
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file_date_updated: 2023-06-26T10:15:44Z
has_accepted_license: '1'
intvolume: ' 29'
isi: 1
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
page: 1456-1467
publication: Nature Medicine
publication_identifier:
eissn:
- 1546-170X
issn:
- 1078-8956
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Effects of urban living environments on mental health in adults
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 29
year: '2023'
...
---
_id: '14455'
acknowledgement: The author(s) declare that financial support was received for the
research, authorship, and/or publication of this article. This work has been partially
supported by Italian Ministry of Health Grant RC2023 (and the 5 × 1,000 voluntary
contributions). The authors thank the children and their families with whom they
work daily.
article_number: '1287879'
article_processing_charge: Yes
article_type: letter_note
author:
- first_name: Antonio
full_name: Narzisi, Antonio
last_name: Narzisi
- first_name: Alycia
full_name: Halladay, Alycia
last_name: Halladay
- first_name: Gabriele
full_name: Masi, Gabriele
last_name: Masi
- first_name: Gaia
full_name: Novarino, Gaia
id: 3E57A680-F248-11E8-B48F-1D18A9856A87
last_name: Novarino
orcid: 0000-0002-7673-7178
- first_name: Catherine
full_name: Lord, Catherine
last_name: Lord
citation:
ama: 'Narzisi A, Halladay A, Masi G, Novarino G, Lord C. Tempering expectations:
Considerations on the current state of stem cells therapy for autism treatment.
Frontiers in Psychiatry. 2023;14. doi:10.3389/fpsyt.2023.1287879'
apa: 'Narzisi, A., Halladay, A., Masi, G., Novarino, G., & Lord, C. (2023).
Tempering expectations: Considerations on the current state of stem cells therapy
for autism treatment. Frontiers in Psychiatry. Frontiers. https://doi.org/10.3389/fpsyt.2023.1287879'
chicago: 'Narzisi, Antonio, Alycia Halladay, Gabriele Masi, Gaia Novarino, and Catherine
Lord. “Tempering Expectations: Considerations on the Current State of Stem Cells
Therapy for Autism Treatment.” Frontiers in Psychiatry. Frontiers, 2023.
https://doi.org/10.3389/fpsyt.2023.1287879.'
ieee: 'A. Narzisi, A. Halladay, G. Masi, G. Novarino, and C. Lord, “Tempering expectations:
Considerations on the current state of stem cells therapy for autism treatment,”
Frontiers in Psychiatry, vol. 14. Frontiers, 2023.'
ista: 'Narzisi A, Halladay A, Masi G, Novarino G, Lord C. 2023. Tempering expectations:
Considerations on the current state of stem cells therapy for autism treatment.
Frontiers in Psychiatry. 14, 1287879.'
mla: 'Narzisi, Antonio, et al. “Tempering Expectations: Considerations on the Current
State of Stem Cells Therapy for Autism Treatment.” Frontiers in Psychiatry,
vol. 14, 1287879, Frontiers, 2023, doi:10.3389/fpsyt.2023.1287879.'
short: A. Narzisi, A. Halladay, G. Masi, G. Novarino, C. Lord, Frontiers in Psychiatry
14 (2023).
date_created: 2023-10-29T23:01:16Z
date_published: 2023-10-03T00:00:00Z
date_updated: 2023-12-13T13:06:07Z
day: '03'
ddc:
- '570'
department:
- _id: GaNo
doi: 10.3389/fpsyt.2023.1287879
external_id:
isi:
- '001084841700001'
pmid:
- '37854442'
file:
- access_level: open_access
checksum: 0a76373e9a4c0fc199f80380de257e86
content_type: application/pdf
creator: dernst
date_created: 2023-10-30T12:48:40Z
date_updated: 2023-10-30T12:48:40Z
file_id: '14468'
file_name: 2023_FrontiersPsychiatry_Narzisi.pdf
file_size: 147878
relation: main_file
success: 1
file_date_updated: 2023-10-30T12:48:40Z
has_accepted_license: '1'
intvolume: ' 14'
isi: 1
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
pmid: 1
publication: Frontiers in Psychiatry
publication_identifier:
eissn:
- 1664-0640
publication_status: published
publisher: Frontiers
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Tempering expectations: Considerations on the current state of stem cells
therapy for autism treatment'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 14
year: '2023'
...
---
_id: '13267'
abstract:
- lang: eng
text: Three-dimensional (3D) reconstruction of living brain tissue down to an individual
synapse level would create opportunities for decoding the dynamics and structure–function
relationships of the brain’s complex and dense information processing network;
however, this has been hindered by insufficient 3D resolution, inadequate signal-to-noise
ratio and prohibitive light burden in optical imaging, whereas electron microscopy
is inherently static. Here we solved these challenges by developing an integrated
optical/machine-learning technology, LIONESS (live information-optimized nanoscopy
enabling saturated segmentation). This leverages optical modifications to stimulated
emission depletion microscopy in comprehensively, extracellularly labeled tissue
and previous information on sample structure via machine learning to simultaneously
achieve isotropic super-resolution, high signal-to-noise ratio and compatibility
with living tissue. This allows dense deep-learning-based instance segmentation
and 3D reconstruction at a synapse level, incorporating molecular, activity and
morphodynamic information. LIONESS opens up avenues for studying the dynamic functional
(nano-)architecture of living brain tissue.
acknowledged_ssus:
- _id: ScienComp
- _id: Bio
- _id: PreCl
- _id: E-Lib
- _id: LifeSc
- _id: M-Shop
acknowledgement: "We thank J. Vorlaufer, N. Agudelo and A. Wartak for microscope maintenance
and troubleshooting, C. Kreuzinger and A. Freeman for technical assistance, M. Šuplata
for hardware control support and M. Cunha dos Santos for initial exploration of
software. We\r\nthank P. Henderson for advice on deep-learning training and M. Sixt,
S. Boyd and T. Weiss for discussions and critical reading of the manuscript. L.
Lavis (Janelia Research Campus) generously provided the JF585-HaloTag ligand. We
acknowledge expert support by IST\r\nAustria’s scientific computing, imaging and
optics, preclinical, library and laboratory support facilities and by the Miba machine
shop. We gratefully acknowledge funding by the following sources: Austrian Science
Fund (F.W.F.) grant no. I3600-B27 (J.G.D.), grant no. DK W1232\r\n(J.G.D. and J.M.M.)
and grant no. Z 312-B27, Wittgenstein award (P.J.); the Gesellschaft für Forschungsförderung
NÖ grant no. LSC18-022 (J.G.D.); an ISTA Interdisciplinary project grant (J.G.D.
and B.B.); the European Union’s Horizon 2020 research and innovation programme,\r\nMarie-Skłodowska
Curie grant 665385 (J.M.M. and J.L.); the European Union’s Horizon 2020 research
and innovation programme, European Research Council grant no. 715767, MATERIALIZABLE
(B.B.); grant no. 715508, REVERSEAUTISM (G.N.); grant no. 695568, SYNNOVATE (S.G.N.G.);
and grant no. 692692, GIANTSYN (P.J.); the Simons\r\nFoundation Autism Research
Initiative grant no. 529085 (S.G.N.G.); the Wellcome Trust Technology Development
grant no. 202932 (S.G.N.G.); the Marie Skłodowska-Curie Actions Individual Fellowship
no. 101026635 under the EU Horizon 2020 program (J.F.W.);\r\nthe Human Frontier
Science Program postdoctoral fellowship LT000557/2018 (W.J.); and the National Science
Foundation grant no. IIS-1835231 (H.P.) and NCS-FO-2124179 (H.P.)."
article_processing_charge: Yes
article_type: original
author:
- first_name: Philipp
full_name: Velicky, Philipp
id: 39BDC62C-F248-11E8-B48F-1D18A9856A87
last_name: Velicky
orcid: 0000-0002-2340-7431
- first_name: Eder
full_name: Miguel Villalba, Eder
id: 3FB91342-F248-11E8-B48F-1D18A9856A87
last_name: Miguel Villalba
orcid: 0000-0001-5665-0430
- first_name: Julia M
full_name: Michalska, Julia M
id: 443DB6DE-F248-11E8-B48F-1D18A9856A87
last_name: Michalska
orcid: 0000-0003-3862-1235
- first_name: Julia
full_name: Lyudchik, Julia
id: 46E28B80-F248-11E8-B48F-1D18A9856A87
last_name: Lyudchik
- first_name: Donglai
full_name: Wei, Donglai
last_name: Wei
- first_name: Zudi
full_name: Lin, Zudi
last_name: Lin
- first_name: Jake
full_name: Watson, Jake
id: 63836096-4690-11EA-BD4E-32803DDC885E
last_name: Watson
orcid: 0000-0002-8698-3823
- first_name: Jakob
full_name: Troidl, Jakob
last_name: Troidl
- first_name: Johanna
full_name: Beyer, Johanna
last_name: Beyer
- first_name: Yoav
full_name: Ben Simon, Yoav
id: 43DF3136-F248-11E8-B48F-1D18A9856A87
last_name: Ben Simon
- first_name: Christoph M
full_name: Sommer, Christoph M
id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
last_name: Sommer
orcid: 0000-0003-1216-9105
- first_name: Wiebke
full_name: Jahr, Wiebke
id: 425C1CE8-F248-11E8-B48F-1D18A9856A87
last_name: Jahr
- first_name: Alban
full_name: Cenameri, Alban
id: 9ac8f577-2357-11eb-997a-e566c5550886
last_name: Cenameri
- first_name: Johannes
full_name: Broichhagen, Johannes
last_name: Broichhagen
- first_name: Seth G.N.
full_name: Grant, Seth G.N.
last_name: Grant
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: Gaia
full_name: Novarino, Gaia
id: 3E57A680-F248-11E8-B48F-1D18A9856A87
last_name: Novarino
orcid: 0000-0002-7673-7178
- first_name: Hanspeter
full_name: Pfister, Hanspeter
last_name: Pfister
- first_name: Bernd
full_name: Bickel, Bernd
id: 49876194-F248-11E8-B48F-1D18A9856A87
last_name: Bickel
orcid: 0000-0001-6511-9385
- first_name: Johann G
full_name: Danzl, Johann G
id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87
last_name: Danzl
orcid: 0000-0001-8559-3973
citation:
ama: Velicky P, Miguel Villalba E, Michalska JM, et al. Dense 4D nanoscale reconstruction
of living brain tissue. Nature Methods. 2023;20:1256-1265. doi:10.1038/s41592-023-01936-6
apa: Velicky, P., Miguel Villalba, E., Michalska, J. M., Lyudchik, J., Wei, D.,
Lin, Z., … Danzl, J. G. (2023). Dense 4D nanoscale reconstruction of living brain
tissue. Nature Methods. Springer Nature. https://doi.org/10.1038/s41592-023-01936-6
chicago: Velicky, Philipp, Eder Miguel Villalba, Julia M Michalska, Julia Lyudchik,
Donglai Wei, Zudi Lin, Jake Watson, et al. “Dense 4D Nanoscale Reconstruction
of Living Brain Tissue.” Nature Methods. Springer Nature, 2023. https://doi.org/10.1038/s41592-023-01936-6.
ieee: P. Velicky et al., “Dense 4D nanoscale reconstruction of living brain
tissue,” Nature Methods, vol. 20. Springer Nature, pp. 1256–1265, 2023.
ista: Velicky P, Miguel Villalba E, Michalska JM, Lyudchik J, Wei D, Lin Z, Watson
J, Troidl J, Beyer J, Ben Simon Y, Sommer CM, Jahr W, Cenameri A, Broichhagen
J, Grant SGN, Jonas PM, Novarino G, Pfister H, Bickel B, Danzl JG. 2023. Dense
4D nanoscale reconstruction of living brain tissue. Nature Methods. 20, 1256–1265.
mla: Velicky, Philipp, et al. “Dense 4D Nanoscale Reconstruction of Living Brain
Tissue.” Nature Methods, vol. 20, Springer Nature, 2023, pp. 1256–65, doi:10.1038/s41592-023-01936-6.
short: P. Velicky, E. Miguel Villalba, J.M. Michalska, J. Lyudchik, D. Wei, Z. Lin,
J. Watson, J. Troidl, J. Beyer, Y. Ben Simon, C.M. Sommer, W. Jahr, A. Cenameri,
J. Broichhagen, S.G.N. Grant, P.M. Jonas, G. Novarino, H. Pfister, B. Bickel,
J.G. Danzl, Nature Methods 20 (2023) 1256–1265.
date_created: 2023-07-23T22:01:13Z
date_published: 2023-08-01T00:00:00Z
date_updated: 2024-01-10T08:37:48Z
day: '01'
department:
- _id: PeJo
- _id: GaNo
- _id: BeBi
- _id: JoDa
- _id: Bio
doi: 10.1038/s41592-023-01936-6
ec_funded: 1
external_id:
isi:
- '001025621500001'
pmid:
- '37429995'
intvolume: ' 20'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1038/s41592-023-01936-6
month: '08'
oa: 1
oa_version: Published Version
page: 1256-1265
pmid: 1
project:
- _id: 265CB4D0-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03600
name: Optical control of synaptic function via adhesion molecules
- _id: 2548AE96-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: W1232-B24
name: Molecular Drug Targets
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: The Wittgenstein Prize
- _id: 23889792-32DE-11EA-91FC-C7463DDC885E
name: High content imaging to decode human immune cell interactions in health and
allergic disease
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
- _id: 24F9549A-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '715767'
name: 'MATERIALIZABLE: Intelligent fabrication-oriented Computational Design and
Modeling'
- _id: 25444568-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '715508'
name: Probing the Reversibility of Autism Spectrum Disorders by Employing in vivo
and in vitro Models
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: fc2be41b-9c52-11eb-aca3-faa90aa144e9
call_identifier: H2020
grant_number: '101026635'
name: Synaptic computations of the hippocampal CA3 circuitry
- _id: 2668BFA0-B435-11E9-9278-68D0E5697425
grant_number: LT00057
name: High-speed 3D-nanoscopy to study the role of adhesion during 3D cell migration
publication: Nature Methods
publication_identifier:
eissn:
- 1548-7105
issn:
- 1548-7091
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- relation: software
url: https://github.com/danzllab/LIONESS
record:
- id: '12817'
relation: research_data
status: public
- id: '14770'
relation: shorter_version
status: public
scopus_import: '1'
status: public
title: Dense 4D nanoscale reconstruction of living brain tissue
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 20
year: '2023'
...