[{"project":[{"_id":"9B954C5C-BA93-11EA-9121-9846C619BF3A","grant_number":"P33367","name":"Structure and isoform diversity of the Arp2/3 complex"}],"title":"Research data of the publication \"ArpC5 isoforms regulate Arp2/3 complex-dependent protrusion through differential Ena/VASP positioning\"","article_processing_charge":"No","author":[{"first_name":"Florian KM","id":"48AD8942-F248-11E8-B48F-1D18A9856A87","last_name":"Schur","full_name":"Schur, Florian KM","orcid":"0000-0003-4790-8078"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"mla":"Schur, Florian KM. Research Data of the Publication “ArpC5 Isoforms Regulate Arp2/3 Complex-Dependent Protrusion through Differential Ena/VASP Positioning.” Institute of Science and Technology Austria, 2023, doi:10.15479/AT:ISTA:14562.","ieee":"F. K. Schur, “Research data of the publication ‘ArpC5 isoforms regulate Arp2/3 complex-dependent protrusion through differential Ena/VASP positioning.’” Institute of Science and Technology Austria, 2023.","short":"F.K. Schur, (2023).","apa":"Schur, F. K. (2023). Research data of the publication “ArpC5 isoforms regulate Arp2/3 complex-dependent protrusion through differential Ena/VASP positioning.” Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:14562","ama":"Schur FK. Research data of the publication “ArpC5 isoforms regulate Arp2/3 complex-dependent protrusion through differential Ena/VASP positioning.” 2023. doi:10.15479/AT:ISTA:14562","chicago":"Schur, Florian KM. “Research Data of the Publication ‘ArpC5 Isoforms Regulate Arp2/3 Complex-Dependent Protrusion through Differential Ena/VASP Positioning.’” Institute of Science and Technology Austria, 2023. https://doi.org/10.15479/AT:ISTA:14562.","ista":"Schur FK. 2023. Research data of the publication ‘ArpC5 isoforms regulate Arp2/3 complex-dependent protrusion through differential Ena/VASP positioning’, Institute of Science and Technology Austria, 10.15479/AT:ISTA:14562."},"oa":1,"publisher":"Institute of Science and Technology Austria","acknowledgement":"We would like to thank K. von Peinen and B. Denker (Helmholtz Centre for Infection Research, Braunschweig, Germany) for experimental and technical assistance, respectively.\r\nFunding: This research was supported by the Scientific Service Units (SSUs) of ISTA through resources provided by Scientific Computing (SciComp), the Life Science Facility (LSF), the Imaging and Optics facility (IOF), and the Electron Microscopy Facility (EMF). We acknowledge support from ISTA and from the Austrian Science Fund (FWF) (P33367) to F.K.M.S., from the Research Training Group GRK2223 and the Helmholtz Society to K.R,. and from the Deutsche Forschungsgemeinschaft (DFG) to J.F. and K.R.","date_created":"2023-11-20T09:22:33Z","doi":"10.15479/AT:ISTA:14562","date_published":"2023-11-21T00:00:00Z","day":"21","year":"2023","has_accepted_license":"1","status":"public","tmp":{"short":"CC BY-SA (4.0)","image":"/images/cc_by_sa.png","legal_code_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode","name":"Creative Commons Attribution-ShareAlike 4.0 International Public License (CC BY-SA 4.0)"},"type":"research_data","_id":"14562","file_date_updated":"2023-11-20T11:49:58Z","department":[{"_id":"FlSc"}],"ddc":["570"],"date_updated":"2023-11-21T08:05:34Z","month":"11","oa_version":"Published Version","abstract":[{"text":"Regulation of the Arp2/3 complex is required for productive nucleation of branched actin networks. An emerging aspect of regulation is the incorporation of subunit isoforms into the Arp2/3 complex. Specifically, both ArpC5 subunit isoforms, ArpC5 and ArpC5L, have been reported to fine-tune nucleation activity and branch junction stability. We have combined reverse genetics and cellular structural biology to describe how ArpC5 and ArpC5L differentially affect cell migration. Both define the structural stability of ArpC1 in branch junctions and, in turn, by determining protrusion characteristics, affect protein dynamics and actin network ultrastructure. ArpC5 isoforms also affect the positioning of members of the Ena/Vasodilator-stimulated phosphoprotein (VASP) family of actin filament elongators, which mediate ArpC5 isoform–specific effects on the actin assembly level. Our results suggest that ArpC5 and Ena/VASP proteins are part of a signaling pathway enhancing cell migration.\r\n","lang":"eng"}],"acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"},{"_id":"ScienComp"},{"_id":"EM-Fac"}],"license":"https://creativecommons.org/licenses/by-sa/4.0/","contributor":[{"first_name":"Florian","id":"404F5528-F248-11E8-B48F-1D18A9856A87","contributor_type":"researcher","orcid":"0000-0001-7149-769X","last_name":"Fäßler"},{"last_name":"Javoor","contributor_type":"researcher","first_name":"Manjunath","id":"305ab18b-dc7d-11ea-9b2f-b58195228ea2"},{"orcid":"0000-0002-3616-8580","last_name":"Datler","contributor_type":"researcher","id":"3B12E2E6-F248-11E8-B48F-1D18A9856A87","first_name":"Julia"},{"last_name":"Döring","contributor_type":"researcher","first_name":"Hermann"},{"id":"b9d234ba-9e33-11ed-95b6-cd561df280e6","contributor_type":"researcher","first_name":"Florian","last_name":"Hofer"},{"contributor_type":"researcher","first_name":"Georgi A","id":"38C393BE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8370-6161","last_name":"Dimchev"},{"last_name":"Hodirnau","first_name":"Victor-Valentin","id":"3661B498-F248-11E8-B48F-1D18A9856A87","contributor_type":"researcher"},{"last_name":"Faix","first_name":"Jan","contributor_type":"researcher"},{"last_name":"Rottner","first_name":"Klemens","contributor_type":"researcher"},{"first_name":"Florian KM","contributor_type":"researcher","id":"48AD8942-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4790-8078","last_name":"Schur"}],"related_material":{"record":[{"status":"public","id":"12334","relation":"used_in_publication"}]},"file":[{"content_type":"application/zip","relation":"main_file","access_level":"open_access","success":1,"checksum":"e9bab797b44614f144a5b02d9636f8c3","file_id":"14570","file_size":1581687449,"date_updated":"2023-11-20T10:27:17Z","creator":"fschur","file_name":"Figure2.zip","date_created":"2023-11-20T10:27:17Z"},{"success":1,"file_id":"14571","checksum":"4efd388cccd03c549fc90f6e46d37006","relation":"main_file","access_level":"open_access","content_type":"application/zip","file_name":"SupplementaryFigure3.zip","date_created":"2023-11-20T10:29:18Z","creator":"fschur","file_size":116088565,"date_updated":"2023-11-20T10:29:18Z"},{"date_created":"2023-11-20T10:44:39Z","file_name":"Figure5.zip","date_updated":"2023-11-20T10:44:39Z","file_size":5154614201,"creator":"fschur","checksum":"bdeb232dc94d0c22a3f7e0d18189ce89","file_id":"14572","success":1,"content_type":"application/zip","access_level":"open_access","relation":"main_file"},{"content_type":"application/zip","access_level":"open_access","relation":"main_file","file_id":"14573","checksum":"83aee17d621a05d865f68f39c8892d27","success":1,"date_updated":"2023-11-20T10:46:00Z","file_size":1277893286,"creator":"fschur","date_created":"2023-11-20T10:46:00Z","file_name":"SupplementaryFigure7.zip"},{"date_created":"2023-11-20T10:46:08Z","file_name":"SupplementaryFigure9.zip","date_updated":"2023-11-20T10:46:08Z","file_size":228485124,"creator":"fschur","file_id":"14574","checksum":"fb9beb6fe15c8dac6679dd02044d2ea6","success":1,"content_type":"application/zip","access_level":"open_access","relation":"main_file"},{"file_name":"SupplementaryFigure10.zip","date_created":"2023-11-20T10:46:32Z","file_size":1226788198,"date_updated":"2023-11-20T10:46:32Z","creator":"fschur","success":1,"file_id":"14575","checksum":"4f3644e5feabe4824486d56885bb79fe","content_type":"application/zip","relation":"main_file","access_level":"open_access"},{"file_name":"SupplementaryFigure11.zip","date_created":"2023-11-20T10:46:17Z","file_size":277577131,"date_updated":"2023-11-20T10:46:17Z","creator":"fschur","success":1,"file_id":"14576","checksum":"96167f722ed0ca78e30681cd1573b9d7","content_type":"application/zip","relation":"main_file","access_level":"open_access"},{"date_created":"2023-11-20T10:46:29Z","file_name":"SupplementaryFigure15.zip","date_updated":"2023-11-20T10:46:29Z","file_size":591483468,"creator":"fschur","checksum":"d1e03c9805c18cfbc2e9fdf38a9f556f","file_id":"14577","success":1,"content_type":"application/zip","access_level":"open_access","relation":"main_file"},{"success":1,"file_id":"14578","checksum":"4d437c04fdb3c1e699618063c4bd21c3","content_type":"application/zip","relation":"main_file","access_level":"open_access","file_name":"SupplementaryFigure17.zip","date_created":"2023-11-20T10:47:00Z","file_size":1709528579,"date_updated":"2023-11-20T10:47:00Z","creator":"fschur"},{"creator":"fschur","file_size":1920765280,"date_updated":"2023-11-20T11:26:36Z","file_name":"SupplementaryFigure4.zip","date_created":"2023-11-20T11:26:36Z","relation":"main_file","access_level":"open_access","content_type":"application/zip","success":1,"file_id":"14581","checksum":"967b5378a4f16c43f490eae328afe50e"},{"file_size":3013566196,"date_updated":"2023-11-20T11:38:12Z","creator":"fschur","file_name":"Figure1_partA.zip","date_created":"2023-11-20T11:38:12Z","content_type":"application/zip","relation":"main_file","access_level":"open_access","success":1,"file_id":"14583","checksum":"11899986cf0b471d258fe168ee33a3ea"},{"relation":"main_file","access_level":"open_access","content_type":"application/zip","success":1,"file_id":"14584","checksum":"c452afe1ab506d58d32e601d5b3878bb","creator":"fschur","file_size":3250260203,"date_updated":"2023-11-20T11:43:23Z","file_name":"Figure1_partB.zip","date_created":"2023-11-20T11:43:23Z"},{"file_name":"ReadMe.rtf","date_created":"2023-11-20T11:49:58Z","file_size":1460,"date_updated":"2023-11-20T11:49:58Z","creator":"fschur","success":1,"checksum":"223c98eceecbe65dd268f4f363a620d8","file_id":"14585","content_type":"text/rtf","relation":"main_file","access_level":"open_access"}]},{"_id":"14592","status":"public","project":[{"_id":"9B954C5C-BA93-11EA-9121-9846C619BF3A","name":"Structure and isoform diversity of the Arp2/3 complex","grant_number":"P33367"}],"type":"research_data","tmp":{"name":"Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)","image":"/images/cc_by_nc_sa.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode","short":"CC BY-NC-SA (4.0)"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","ddc":["570"],"date_updated":"2024-02-21T12:44:48Z","citation":{"ista":"Schur FK. 2020. STL-files for 3D-printed grid holders described in Fäßler F, Zens B, et al.; 3D printed cell culture grid holders for improved cellular specimen preparation in cryo-electron microscopy, Institute of Science and Technology Austria, 10.15479/AT:ISTA:14592.","chicago":"Schur, Florian KM. “STL-Files for 3D-Printed Grid Holders Described in Fäßler F, Zens B, et Al.; 3D Printed Cell Culture Grid Holders for Improved Cellular Specimen Preparation in Cryo-Electron Microscopy.” Institute of Science and Technology Austria, 2020. https://doi.org/10.15479/AT:ISTA:14592.","ama":"Schur FK. STL-files for 3D-printed grid holders described in Fäßler F, Zens B, et al.; 3D printed cell culture grid holders for improved cellular specimen preparation in cryo-electron microscopy. 2020. doi:10.15479/AT:ISTA:14592","apa":"Schur, F. K. (2020). STL-files for 3D-printed grid holders described in Fäßler F, Zens B, et al.; 3D printed cell culture grid holders for improved cellular specimen preparation in cryo-electron microscopy. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:14592","short":"F.K. Schur, (2020).","ieee":"F. K. Schur, “STL-files for 3D-printed grid holders described in Fäßler F, Zens B, et al.; 3D printed cell culture grid holders for improved cellular specimen preparation in cryo-electron microscopy.” Institute of Science and Technology Austria, 2020.","mla":"Schur, Florian KM. STL-Files for 3D-Printed Grid Holders Described in Fäßler F, Zens B, et Al.; 3D Printed Cell Culture Grid Holders for Improved Cellular Specimen Preparation in Cryo-Electron Microscopy. Institute of Science and Technology Austria, 2020, doi:10.15479/AT:ISTA:14592."},"file_date_updated":"2023-12-01T10:39:59Z","department":[{"_id":"FlSc"}],"title":"STL-files for 3D-printed grid holders described in Fäßler F, Zens B, et al.; 3D printed cell culture grid holders for improved cellular specimen preparation in cryo-electron microscopy","author":[{"first_name":"Florian KM","id":"48AD8942-F248-11E8-B48F-1D18A9856A87","last_name":"Schur","full_name":"Schur, Florian KM","orcid":"0000-0003-4790-8078"}],"article_processing_charge":"No","oa_version":"Published Version","abstract":[{"text":"Cryo-electron microscopy (cryo-EM) of cellular specimens provides insights into biological processes and structures within a native context. However, a major challenge still lies in the efficient and reproducible preparation of adherent cells for subsequent cryo-EM analysis. This is due to the sensitivity of many cellular specimens to the varying seeding and culturing conditions required for EM experiments, the often limited amount of cellular material and also the fragility of EM grids and their substrate. Here, we present low-cost and reusable 3D printed grid holders, designed to improve specimen preparation when culturing challenging cellular samples directly on grids. The described grid holders increase cell culture reproducibility and throughput, and reduce the resources required for cell culturing. We show that grid holders can be integrated into various cryo-EM workflows, including micro-patterning approaches to control cell seeding on grids, and for generating samples for cryo-focused ion beam milling and cryo-electron tomography experiments. Their adaptable design allows for the generation of specialized grid holders customized to a large variety of applications.","lang":"eng"}],"month":"12","publisher":"Institute of Science and Technology Austria","oa":1,"day":"01","file":[{"content_type":"application/zip","access_level":"open_access","relation":"main_file","file_id":"14593","checksum":"0108616e2a59e51879ea51299a29b091","success":1,"date_updated":"2023-11-22T14:58:44Z","file_size":49297,"creator":"fschur","date_created":"2023-11-22T14:58:44Z","file_name":"3Dprint-files_download_v2.zip"},{"file_name":"readme.txt","date_created":"2023-12-01T10:39:59Z","creator":"cchlebak","file_size":641,"date_updated":"2023-12-01T10:39:59Z","success":1,"checksum":"4c66ddedee4d01c1c4a7978208350cfc","file_id":"14637","relation":"main_file","access_level":"open_access","content_type":"text/plain"}],"has_accepted_license":"1","year":"2020","date_published":"2020-12-01T00:00:00Z","related_material":{"record":[{"id":"8586","status":"public","relation":"research_data"}]},"doi":"10.15479/AT:ISTA:14592","date_created":"2023-11-22T15:00:57Z","contributor":[{"contributor_type":"researcher","id":"404F5528-F248-11E8-B48F-1D18A9856A87","first_name":"Florian","orcid":"0000-0001-7149-769X","last_name":"Fäßler"},{"contributor_type":"researcher","id":"45FD126C-F248-11E8-B48F-1D18A9856A87","first_name":"Bettina","last_name":"Zens"},{"last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","contributor_type":"researcher"},{"id":"48AD8942-F248-11E8-B48F-1D18A9856A87","contributor_type":"researcher","first_name":"Florian KM","orcid":"0000-0003-4790-8078","last_name":"Schur"}]}]