---
_id: '8924'
abstract:
- lang: eng
text: 'Maintaining fertility in a fluctuating environment is key to the reproductive
success of flowering plants. Meiosis and pollen formation are particularly sensitive
to changes in growing conditions, especially temperature. We have previously identified
cyclin-dependent kinase G1 (CDKG1) as a master regulator of temperature-dependent
meiosis and this may involve the regulation of alternative splicing (AS), including
of its own transcript. CDKG1 mRNA can undergo several AS events, potentially producing
two protein variants: CDKG1L and CDKG1S, differing in their N-terminal domain
which may be involved in co-factor interaction. In leaves, both isoforms have
distinct temperature-dependent functions on target mRNA processing, but their
role in pollen development is unknown. In the present study, we characterize the
role of CDKG1L and CDKG1S in maintaining Arabidopsis fertility. We show that the
long (L) form is necessary and sufficient to rescue the fertility defects of the
cdkg1-1 mutant, while the short (S) form is unable to rescue fertility. On the
other hand, an extra copy of CDKG1L reduces fertility. In addition, mutation of
the ATP binding pocket of the kinase indicates that kinase activity is necessary
for the function of CDKG1. Kinase mutants of CDKG1L and CDKG1S correctly localize
to the cell nucleus and nucleus and cytoplasm, respectively, but are unable to
rescue either the fertility or the splicing defects of the cdkg1-1 mutant. Furthermore,
we show that there is partial functional overlap between CDKG1 and its paralog
CDKG2 that could in part be explained by overlapping gene expression.'
acknowledgement: CN, DD, NF-F, and JD were funded by the BBSRC (grant number BB/M009459/1).
NK and AM were funded through the ERASMUS+Program. NC was funded by the VIPS Program
of the Austrian Federal Ministry of Science and Research and the City of Vienna.
article_number: '586870'
article_processing_charge: No
article_type: original
author:
- first_name: Candida
full_name: Nibau, Candida
last_name: Nibau
- first_name: Despoina
full_name: Dadarou, Despoina
last_name: Dadarou
- first_name: Nestoras
full_name: Kargios, Nestoras
last_name: Kargios
- first_name: Areti
full_name: Mallioura, Areti
last_name: Mallioura
- first_name: Narcis
full_name: Fernandez-Fuentes, Narcis
last_name: Fernandez-Fuentes
- first_name: Nicola
full_name: Cavallari, Nicola
id: 457160E6-F248-11E8-B48F-1D18A9856A87
last_name: Cavallari
- first_name: John H.
full_name: Doonan, John H.
last_name: Doonan
citation:
ama: Nibau C, Dadarou D, Kargios N, et al. A functional kinase is necessary for
cyclin-dependent kinase G1 (CDKG1) to maintain fertility at high ambient temperature
in Arabidopsis. Frontiers in Plant Science. 2020;11. doi:10.3389/fpls.2020.586870
apa: Nibau, C., Dadarou, D., Kargios, N., Mallioura, A., Fernandez-Fuentes, N.,
Cavallari, N., & Doonan, J. H. (2020). A functional kinase is necessary for
cyclin-dependent kinase G1 (CDKG1) to maintain fertility at high ambient temperature
in Arabidopsis. Frontiers in Plant Science. Frontiers. https://doi.org/10.3389/fpls.2020.586870
chicago: Nibau, Candida, Despoina Dadarou, Nestoras Kargios, Areti Mallioura, Narcis
Fernandez-Fuentes, Nicola Cavallari, and John H. Doonan. “A Functional Kinase
Is Necessary for Cyclin-Dependent Kinase G1 (CDKG1) to Maintain Fertility at High
Ambient Temperature in Arabidopsis.” Frontiers in Plant Science. Frontiers,
2020. https://doi.org/10.3389/fpls.2020.586870.
ieee: C. Nibau et al., “A functional kinase is necessary for cyclin-dependent
kinase G1 (CDKG1) to maintain fertility at high ambient temperature in Arabidopsis,”
Frontiers in Plant Science, vol. 11. Frontiers, 2020.
ista: Nibau C, Dadarou D, Kargios N, Mallioura A, Fernandez-Fuentes N, Cavallari
N, Doonan JH. 2020. A functional kinase is necessary for cyclin-dependent kinase
G1 (CDKG1) to maintain fertility at high ambient temperature in Arabidopsis. Frontiers
in Plant Science. 11, 586870.
mla: Nibau, Candida, et al. “A Functional Kinase Is Necessary for Cyclin-Dependent
Kinase G1 (CDKG1) to Maintain Fertility at High Ambient Temperature in Arabidopsis.”
Frontiers in Plant Science, vol. 11, 586870, Frontiers, 2020, doi:10.3389/fpls.2020.586870.
short: C. Nibau, D. Dadarou, N. Kargios, A. Mallioura, N. Fernandez-Fuentes, N.
Cavallari, J.H. Doonan, Frontiers in Plant Science 11 (2020).
date_created: 2020-12-06T23:01:14Z
date_published: 2020-11-10T00:00:00Z
date_updated: 2023-08-24T10:50:00Z
day: '10'
ddc:
- '580'
department:
- _id: EvBe
doi: 10.3389/fpls.2020.586870
external_id:
isi:
- '000591637000001'
file:
- access_level: open_access
checksum: 1c0ee6ce9950aa665d6a5cc64aa6b752
content_type: application/pdf
creator: dernst
date_created: 2020-12-09T09:14:19Z
date_updated: 2020-12-09T09:14:19Z
file_id: '8929'
file_name: 2020_Frontiers_Nibau.pdf
file_size: 1833244
relation: main_file
success: 1
file_date_updated: 2020-12-09T09:14:19Z
has_accepted_license: '1'
intvolume: ' 11'
isi: 1
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
publication: Frontiers in Plant Science
publication_identifier:
eissn:
- 1664-462X
publication_status: published
publisher: Frontiers
quality_controlled: '1'
scopus_import: '1'
status: public
title: A functional kinase is necessary for cyclin-dependent kinase G1 (CDKG1) to
maintain fertility at high ambient temperature in Arabidopsis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 11
year: '2020'
...
---
_id: '8142'
abstract:
- lang: eng
text: Cell production and differentiation for the acquisition of specific functions
are key features of living systems. The dynamic network of cellular microtubules
provides the necessary platform to accommodate processes associated with the transition
of cells through the individual phases of cytogenesis. Here, we show that the
plant hormone cytokinin fine‐tunes the activity of the microtubular cytoskeleton
during cell differentiation and counteracts microtubular rearrangements driven
by the hormone auxin. The endogenous upward gradient of cytokinin activity along
the longitudinal growth axis in Arabidopsis thaliana roots correlates with robust
rearrangements of the microtubule cytoskeleton in epidermal cells progressing
from the proliferative to the differentiation stage. Controlled increases in cytokinin
activity result in premature re‐organization of the microtubule network from transversal
to an oblique disposition in cells prior to their differentiation, whereas attenuated
hormone perception delays cytoskeleton conversion into a configuration typical
for differentiated cells. Intriguingly, cytokinin can interfere with microtubules
also in animal cells, such as leukocytes, suggesting that a cytokinin‐sensitive
control pathway for the microtubular cytoskeleton may be at least partially conserved
between plant and animal cells.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: We thank Takashi Aoyama, David Alabadi, and Bert De Rybel for sharing
material, Jiří Friml, Maciek Adamowski, and Katerina Schwarzerová for inspiring
discussions, and Martine De Cock for help in preparing the manuscript. This research
was supported by the Scientific Service Units (SSUs) of IST Austria through resources
provided by the Bioimaging Facility (BIF), especially to Robert Hauschild; and the
Life Science Facility (LSF). J.C.M. is the recipient of a EMBO Long‐Term Fellowship
(ALTF number 710‐2016). This work was supported with MEYS CR, project no.CZ.02.1.01/0.0/0.0/16_019/0000738
to J.P., and by the Austrian Science Fund (FWF01_I1774S) to E.B.
article_number: e104238
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Juan C
full_name: Montesinos López, Juan C
id: 310A8E3E-F248-11E8-B48F-1D18A9856A87
last_name: Montesinos López
orcid: 0000-0001-9179-6099
- first_name: A
full_name: Abuzeineh, A
last_name: Abuzeineh
- first_name: Aglaja
full_name: Kopf, Aglaja
id: 31DAC7B6-F248-11E8-B48F-1D18A9856A87
last_name: Kopf
orcid: 0000-0002-2187-6656
- first_name: Alba
full_name: Juanes Garcia, Alba
id: 40F05888-F248-11E8-B48F-1D18A9856A87
last_name: Juanes Garcia
orcid: 0000-0002-1009-9652
- first_name: Krisztina
full_name: Ötvös, Krisztina
id: 29B901B0-F248-11E8-B48F-1D18A9856A87
last_name: Ötvös
orcid: 0000-0002-5503-4983
- first_name: J
full_name: Petrášek, J
last_name: Petrášek
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Montesinos López JC, Abuzeineh A, Kopf A, et al. Phytohormone cytokinin guides
microtubule dynamics during cell progression from proliferative to differentiated
stage. The Embo Journal. 2020;39(17). doi:10.15252/embj.2019104238
apa: Montesinos López, J. C., Abuzeineh, A., Kopf, A., Juanes Garcia, A., Ötvös,
K., Petrášek, J., … Benková, E. (2020). Phytohormone cytokinin guides microtubule
dynamics during cell progression from proliferative to differentiated stage. The
Embo Journal. Embo Press. https://doi.org/10.15252/embj.2019104238
chicago: Montesinos López, Juan C, A Abuzeineh, Aglaja Kopf, Alba Juanes Garcia,
Krisztina Ötvös, J Petrášek, Michael K Sixt, and Eva Benková. “Phytohormone Cytokinin
Guides Microtubule Dynamics during Cell Progression from Proliferative to Differentiated
Stage.” The Embo Journal. Embo Press, 2020. https://doi.org/10.15252/embj.2019104238.
ieee: J. C. Montesinos López et al., “Phytohormone cytokinin guides microtubule
dynamics during cell progression from proliferative to differentiated stage,”
The Embo Journal, vol. 39, no. 17. Embo Press, 2020.
ista: Montesinos López JC, Abuzeineh A, Kopf A, Juanes Garcia A, Ötvös K, Petrášek
J, Sixt MK, Benková E. 2020. Phytohormone cytokinin guides microtubule dynamics
during cell progression from proliferative to differentiated stage. The Embo Journal.
39(17), e104238.
mla: Montesinos López, Juan C., et al. “Phytohormone Cytokinin Guides Microtubule
Dynamics during Cell Progression from Proliferative to Differentiated Stage.”
The Embo Journal, vol. 39, no. 17, e104238, Embo Press, 2020, doi:10.15252/embj.2019104238.
short: J.C. Montesinos López, A. Abuzeineh, A. Kopf, A. Juanes Garcia, K. Ötvös,
J. Petrášek, M.K. Sixt, E. Benková, The Embo Journal 39 (2020).
date_created: 2020-07-21T09:08:38Z
date_published: 2020-09-01T00:00:00Z
date_updated: 2023-09-05T13:05:47Z
day: '01'
ddc:
- '580'
department:
- _id: MiSi
- _id: EvBe
doi: 10.15252/embj.2019104238
external_id:
isi:
- '000548311800001'
pmid:
- '32667089'
file:
- access_level: open_access
checksum: 43d2b36598708e6ab05c69074e191d57
content_type: application/pdf
creator: dernst
date_created: 2020-12-02T09:13:23Z
date_updated: 2020-12-02T09:13:23Z
file_id: '8827'
file_name: 2020_EMBO_Montesinos.pdf
file_size: 3497156
relation: main_file
success: 1
file_date_updated: 2020-12-02T09:13:23Z
has_accepted_license: '1'
intvolume: ' 39'
isi: 1
issue: '17'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 253E54C8-B435-11E9-9278-68D0E5697425
grant_number: ALTF710-2016
name: Molecular mechanism of auxindriven formative divisions delineating lateral
root organogenesis in plants
- _id: 2542D156-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I 1774-B16
name: Hormone cross-talk drives nutrient dependent plant development
publication: The Embo Journal
publication_identifier:
eissn:
- 1460-2075
issn:
- 0261-4189
publication_status: published
publisher: Embo Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Phytohormone cytokinin guides microtubule dynamics during cell progression
from proliferative to differentiated stage
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 39
year: '2020'
...
---
_id: '15037'
abstract:
- lang: eng
text: Protein abundance and localization at the plasma membrane (PM) shapes plant
development and mediates adaptation to changing environmental conditions. It is
regulated by ubiquitination, a post-translational modification crucial for the
proper sorting of endocytosed PM proteins to the vacuole for subsequent degradation.
To understand the significance and the variety of roles played by this reversible
modification, the function of ubiquitin receptors, which translate the ubiquitin
signature into a cellular response, needs to be elucidated. In this study, we
show that TOL (TOM1-like) proteins function in plants as multivalent ubiquitin
receptors, governing ubiquitinated cargo delivery to the vacuole via the conserved
Endosomal Sorting Complex Required for Transport (ESCRT) pathway. TOL2 and TOL6
interact with components of the ESCRT machinery and bind to K63-linked ubiquitin
via two tandemly arranged conserved ubiquitin-binding domains. Mutation of these
domains results not only in a loss of ubiquitin binding but also altered localization,
abolishing TOL6 ubiquitin receptor activity. Function and localization of TOL6
is itself regulated by ubiquitination, whereby TOL6 ubiquitination potentially
modulates degradation of PM-localized cargoes, assisting in the fine-tuning of
the delicate interplay between protein recycling and downregulation. Taken together,
our findings demonstrate the function and regulation of a ubiquitin receptor that
mediates vacuolar degradation of PM proteins in higher plants.
article_processing_charge: No
article_type: original
author:
- first_name: Jeanette
full_name: Moulinier-Anzola, Jeanette
last_name: Moulinier-Anzola
- first_name: Maximilian
full_name: Schwihla, Maximilian
last_name: Schwihla
- first_name: Lucinda
full_name: De-Araújo, Lucinda
last_name: De-Araújo
- first_name: Christina
full_name: Artner, Christina
id: 45DF286A-F248-11E8-B48F-1D18A9856A87
last_name: Artner
- first_name: Lisa
full_name: Jörg, Lisa
last_name: Jörg
- first_name: Nataliia
full_name: Konstantinova, Nataliia
last_name: Konstantinova
- first_name: Christian
full_name: Luschnig, Christian
last_name: Luschnig
- first_name: Barbara
full_name: Korbei, Barbara
last_name: Korbei
citation:
ama: Moulinier-Anzola J, Schwihla M, De-Araújo L, et al. TOLs function as ubiquitin
receptors in the early steps of the ESCRT pathway in higher plants. Molecular
Plant. 2020;13(5):717-731. doi:10.1016/j.molp.2020.02.012
apa: Moulinier-Anzola, J., Schwihla, M., De-Araújo, L., Artner, C., Jörg, L., Konstantinova,
N., … Korbei, B. (2020). TOLs function as ubiquitin receptors in the early steps
of the ESCRT pathway in higher plants. Molecular Plant. Elsevier. https://doi.org/10.1016/j.molp.2020.02.012
chicago: Moulinier-Anzola, Jeanette, Maximilian Schwihla, Lucinda De-Araújo, Christina
Artner, Lisa Jörg, Nataliia Konstantinova, Christian Luschnig, and Barbara Korbei.
“TOLs Function as Ubiquitin Receptors in the Early Steps of the ESCRT Pathway
in Higher Plants.” Molecular Plant. Elsevier, 2020. https://doi.org/10.1016/j.molp.2020.02.012.
ieee: J. Moulinier-Anzola et al., “TOLs function as ubiquitin receptors in
the early steps of the ESCRT pathway in higher plants,” Molecular Plant,
vol. 13, no. 5. Elsevier, pp. 717–731, 2020.
ista: Moulinier-Anzola J, Schwihla M, De-Araújo L, Artner C, Jörg L, Konstantinova
N, Luschnig C, Korbei B. 2020. TOLs function as ubiquitin receptors in the early
steps of the ESCRT pathway in higher plants. Molecular Plant. 13(5), 717–731.
mla: Moulinier-Anzola, Jeanette, et al. “TOLs Function as Ubiquitin Receptors in
the Early Steps of the ESCRT Pathway in Higher Plants.” Molecular Plant,
vol. 13, no. 5, Elsevier, 2020, pp. 717–31, doi:10.1016/j.molp.2020.02.012.
short: J. Moulinier-Anzola, M. Schwihla, L. De-Araújo, C. Artner, L. Jörg, N. Konstantinova,
C. Luschnig, B. Korbei, Molecular Plant 13 (2020) 717–731.
date_created: 2024-02-28T08:55:56Z
date_published: 2020-05-04T00:00:00Z
date_updated: 2024-02-28T12:41:52Z
day: '04'
ddc:
- '580'
department:
- _id: EvBe
doi: 10.1016/j.molp.2020.02.012
external_id:
pmid:
- '32087370'
file:
- access_level: open_access
checksum: c538a5008f7827f62d17d40a3bfabe65
content_type: application/pdf
creator: dernst
date_created: 2024-02-28T12:39:56Z
date_updated: 2024-02-28T12:39:56Z
file_id: '15038'
file_name: 2020_MolecularPlant_MoulinierAnzola.pdf
file_size: 3089212
relation: main_file
success: 1
file_date_updated: 2024-02-28T12:39:56Z
has_accepted_license: '1'
intvolume: ' 13'
issue: '5'
keyword:
- Plant Science
- Molecular Biology
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
page: 717-731
pmid: 1
publication: Molecular Plant
publication_identifier:
issn:
- 1674-2052
publication_status: published
publisher: Elsevier
quality_controlled: '1'
status: public
title: TOLs function as ubiquitin receptors in the early steps of the ESCRT pathway
in higher plants
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 13
year: '2020'
...
---
_id: '8002'
abstract:
- lang: eng
text: Wound healing in plant tissues, consisting of rigid cell wall-encapsulated
cells, represents a considerable challenge and occurs through largely unknown
mechanisms distinct from those in animals. Owing to their inability to migrate,
plant cells rely on targeted cell division and expansion to regenerate wounds.
Strict coordination of these wound-induced responses is essential to ensure efficient,
spatially restricted wound healing. Single-cell tracking by live imaging allowed
us to gain mechanistic insight into the wound perception and coordination of wound
responses after laser-based wounding in Arabidopsis root. We revealed a crucial
contribution of the collapse of damaged cells in wound perception and detected
an auxin increase specific to cells immediately adjacent to the wound. This localized
auxin increase balances wound-induced cell expansion and restorative division
rates in a dose-dependent manner, leading to tumorous overproliferation when the
canonical TIR1 auxin signaling is disrupted. Auxin and wound-induced turgor pressure
changes together also spatially define the activation of key components of regeneration,
such as the transcription regulator ERF115. Our observations suggest that the
wound signaling involves the sensing of collapse of damaged cells and a local
auxin signaling activation to coordinate the downstream transcriptional responses
in the immediate wound vicinity.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
article_number: '202003346'
article_processing_charge: No
article_type: original
author:
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
orcid: 0000-0001-8295-2926
- first_name: Juan C
full_name: Montesinos López, Juan C
id: 310A8E3E-F248-11E8-B48F-1D18A9856A87
last_name: Montesinos López
orcid: 0000-0001-9179-6099
- first_name: Petra
full_name: Marhavá, Petra
id: 44E59624-F248-11E8-B48F-1D18A9856A87
last_name: Marhavá
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Saiko
full_name: Yoshida, Saiko
id: 2E46069C-F248-11E8-B48F-1D18A9856A87
last_name: Yoshida
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Hörmayer L, Montesinos López JC, Marhavá P, Benková E, Yoshida S, Friml J.
Wounding-induced changes in cellular pressure and localized auxin signalling spatially
coordinate restorative divisions in roots. Proceedings of the National Academy
of Sciences. 2020;117(26). doi:10.1073/pnas.2003346117
apa: Hörmayer, L., Montesinos López, J. C., Marhavá, P., Benková, E., Yoshida, S.,
& Friml, J. (2020). Wounding-induced changes in cellular pressure and localized
auxin signalling spatially coordinate restorative divisions in roots. Proceedings
of the National Academy of Sciences. Proceedings of the National Academy of
Sciences. https://doi.org/10.1073/pnas.2003346117
chicago: Hörmayer, Lukas, Juan C Montesinos López, Petra Marhavá, Eva Benková, Saiko
Yoshida, and Jiří Friml. “Wounding-Induced Changes in Cellular Pressure and Localized
Auxin Signalling Spatially Coordinate Restorative Divisions in Roots.” Proceedings
of the National Academy of Sciences. Proceedings of the National Academy of
Sciences, 2020. https://doi.org/10.1073/pnas.2003346117.
ieee: L. Hörmayer, J. C. Montesinos López, P. Marhavá, E. Benková, S. Yoshida, and
J. Friml, “Wounding-induced changes in cellular pressure and localized auxin signalling
spatially coordinate restorative divisions in roots,” Proceedings of the National
Academy of Sciences, vol. 117, no. 26. Proceedings of the National Academy
of Sciences, 2020.
ista: Hörmayer L, Montesinos López JC, Marhavá P, Benková E, Yoshida S, Friml J.
2020. Wounding-induced changes in cellular pressure and localized auxin signalling
spatially coordinate restorative divisions in roots. Proceedings of the National
Academy of Sciences. 117(26), 202003346.
mla: Hörmayer, Lukas, et al. “Wounding-Induced Changes in Cellular Pressure and
Localized Auxin Signalling Spatially Coordinate Restorative Divisions in Roots.”
Proceedings of the National Academy of Sciences, vol. 117, no. 26, 202003346,
Proceedings of the National Academy of Sciences, 2020, doi:10.1073/pnas.2003346117.
short: L. Hörmayer, J.C. Montesinos López, P. Marhavá, E. Benková, S. Yoshida, J.
Friml, Proceedings of the National Academy of Sciences 117 (2020).
date_created: 2020-06-22T13:33:52Z
date_published: 2020-06-30T00:00:00Z
date_updated: 2024-03-28T23:30:10Z
day: '30'
ddc:
- '580'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1073/pnas.2003346117
ec_funded: 1
external_id:
isi:
- '000565729700033'
pmid:
- '32541049'
file:
- access_level: open_access
checksum: 908b09437680181de9990915f2113aca
content_type: application/pdf
creator: dernst
date_created: 2020-06-23T11:30:53Z
date_updated: 2020-07-14T12:48:07Z
file_id: '8009'
file_name: 2020_PNAS_Hoermayer.pdf
file_size: 2407102
relation: main_file
file_date_updated: 2020-07-14T12:48:07Z
has_accepted_license: '1'
intvolume: ' 117'
isi: 1
issue: '26'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '06'
oa: 1
oa_version: None
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 262EF96E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29988
name: RNA-directed DNA methylation in plant development
publication: Proceedings of the National Academy of Sciences
publication_identifier:
eissn:
- 1091-6490
issn:
- 0027-8424
publication_status: published
publisher: Proceedings of the National Academy of Sciences
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/how-wounded-plants-coordinate-their-healing/
record:
- id: '9992'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Wounding-induced changes in cellular pressure and localized auxin signalling
spatially coordinate restorative divisions in roots
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 117
year: '2020'
...
---
_id: '7427'
abstract:
- lang: eng
text: Plants, like other multicellular organisms, survive through a delicate balance
between growth and defense against pathogens. Salicylic acid (SA) is a major defense
signal in plants, and the perception mechanism as well as downstream signaling
activating the immune response are known. Here, we identify a parallel SA signaling
that mediates growth attenuation. SA directly binds to A subunits of protein phosphatase
2A (PP2A), inhibiting activity of this complex. Among PP2A targets, the PIN2 auxin
transporter is hyperphosphorylated in response to SA, leading to changed activity
of this important growth regulator. Accordingly, auxin transport and auxin-mediated
root development, including growth, gravitropic response, and lateral root organogenesis,
are inhibited. This study reveals how SA, besides activating immunity, concomitantly
attenuates growth through crosstalk with the auxin distribution network. Further
analysis of this dual role of SA and characterization of additional SA-regulated
PP2A targets will provide further insights into mechanisms maintaining a balance
between growth and defense.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: "We thank Shigeyuki Betsuyaku (University of Tsukuba), Alison Delong
(Brown University), Xinnian Dong (Duke University), Dolf Weijers (Wageningen University),
Yuelin Zhang (UBC), and Martine Pastuglia (Institut Jean-Pierre Bourgin) for sharing
published materials; Jana Riederer for help with cantharidin physiological analysis;
David Domjan for help with cloning pET28a-PIN2HL; Qing Lu for help with DARTS; Hana
Kozubı´kova´ for technical support on SA derivative synthesis; Zuzana Vondra´ kova´
for technical support with tobacco cells; Lucia Strader (Washington University),
Bert De Rybel (Ghent University), Bartel Vanholme (Ghent University), and Lukas
Mach (BOKU) for helpful discussions; and bioimaging and life science facilities
of IST Austria for continuous support. We gratefully acknowledge the Nottingham
Arabidopsis Stock Center (NASC) for providing T-DNA insertional mutants. The DSC
and SPR instruments were provided by the EQ-BOKU VIBT GmbH and the BOKU Core Facility
for Biomolecular and Cellular Analysis, with help of Irene Schaffner. The research
leading to these results has received funding from the European Union’s Horizon
2020 program (ERC grant agreement no. 742985 to J.F.) and the People Programme (Marie
Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013)
under REA grant agreement no. 291734. S.T. was supported by a European Molecular
Biology Organization (EMBO) long-term postdoctoral fellowship (ALTF 723-2015). O.N.
was supported by the Ministry of Education, Youth and Sports of the Czech Republic
(European Regional Development Fund-Project ‘‘Centre for Experimental Plant Biology’’
no. CZ.02.1.01/0.0/0.0/16_019/0000738). J. Pospısil was supported by European Regional
Development Fund Project ‘‘Centre for Experimental Plant Biology’’\r\n(no. CZ.02.1.01/0.0/0.0/16_019/0000738).
J. Petrasek was supported by EU Operational Programme Prague-Competitiveness (no.
CZ.2.16/3.1.00/21519). "
article_processing_charge: No
article_type: original
author:
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Melinda F
full_name: Abas, Melinda F
id: 3CFB3B1C-F248-11E8-B48F-1D18A9856A87
last_name: Abas
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: Gergely
full_name: Molnar, Gergely
id: 34F1AF46-F248-11E8-B48F-1D18A9856A87
last_name: Molnar
- first_name: Jakub
full_name: Hajny, Jakub
id: 4800CC20-F248-11E8-B48F-1D18A9856A87
last_name: Hajny
orcid: 0000-0003-2140-7195
- first_name: Pavel
full_name: Lasák, Pavel
last_name: Lasák
- first_name: Ivan
full_name: Petřík, Ivan
last_name: Petřík
- first_name: Eugenia
full_name: Russinova, Eugenia
last_name: Russinova
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Ondřej
full_name: Novák, Ondřej
last_name: Novák
- first_name: Jiří
full_name: Pospíšil, Jiří
last_name: Pospíšil
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Tan S, Abas MF, Verstraeten I, et al. Salicylic acid targets protein phosphatase
2A to attenuate growth in plants. Current Biology. 2020;30(3):381-395.e8.
doi:10.1016/j.cub.2019.11.058
apa: Tan, S., Abas, M. F., Verstraeten, I., Glanc, M., Molnar, G., Hajny, J., …
Friml, J. (2020). Salicylic acid targets protein phosphatase 2A to attenuate growth
in plants. Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2019.11.058
chicago: Tan, Shutang, Melinda F Abas, Inge Verstraeten, Matous Glanc, Gergely Molnar,
Jakub Hajny, Pavel Lasák, et al. “Salicylic Acid Targets Protein Phosphatase 2A
to Attenuate Growth in Plants.” Current Biology. Cell Press, 2020. https://doi.org/10.1016/j.cub.2019.11.058.
ieee: S. Tan et al., “Salicylic acid targets protein phosphatase 2A to attenuate
growth in plants,” Current Biology, vol. 30, no. 3. Cell Press, p. 381–395.e8,
2020.
ista: Tan S, Abas MF, Verstraeten I, Glanc M, Molnar G, Hajny J, Lasák P, Petřík
I, Russinova E, Petrášek J, Novák O, Pospíšil J, Friml J. 2020. Salicylic acid
targets protein phosphatase 2A to attenuate growth in plants. Current Biology.
30(3), 381–395.e8.
mla: Tan, Shutang, et al. “Salicylic Acid Targets Protein Phosphatase 2A to Attenuate
Growth in Plants.” Current Biology, vol. 30, no. 3, Cell Press, 2020, p.
381–395.e8, doi:10.1016/j.cub.2019.11.058.
short: S. Tan, M.F. Abas, I. Verstraeten, M. Glanc, G. Molnar, J. Hajny, P. Lasák,
I. Petřík, E. Russinova, J. Petrášek, O. Novák, J. Pospíšil, J. Friml, Current
Biology 30 (2020) 381–395.e8.
date_created: 2020-02-02T23:01:00Z
date_published: 2020-02-03T00:00:00Z
date_updated: 2024-03-28T23:30:38Z
day: '03'
ddc:
- '580'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1016/j.cub.2019.11.058
ec_funded: 1
external_id:
isi:
- '000511287900018'
pmid:
- '31956021'
file:
- access_level: open_access
checksum: 16f7d51fe28f91c21e4896a2028df40b
content_type: application/pdf
creator: dernst
date_created: 2020-09-22T09:51:28Z
date_updated: 2020-09-22T09:51:28Z
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file_name: 2020_CurrentBiology_Tan.pdf
file_size: 5360135
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success: 1
file_date_updated: 2020-09-22T09:51:28Z
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issue: '3'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: 381-395.e8
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
- _id: 256FEF10-B435-11E9-9278-68D0E5697425
grant_number: 723-2015
name: Long Term Fellowship
publication: Current Biology
publication_identifier:
issn:
- '09609822'
publication_status: published
publisher: Cell Press
quality_controlled: '1'
related_material:
record:
- id: '8822'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Salicylic acid targets protein phosphatase 2A to attenuate growth in plants
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 30
year: '2020'
...