---
_id: '14795'
abstract:
- lang: eng
text: Metazoan development relies on the formation and remodeling of cell-cell contacts.
Dynamic reorganization of adhesion receptors and the actomyosin cell cortex in
space and time plays a central role in cell-cell contact formation and maturation.
Nevertheless, how this process is mechanistically achieved when new contacts are
formed remains unclear. Here, by building a biomimetic assay composed of progenitor
cells adhering to supported lipid bilayers functionalized with E-cadherin ectodomains,
we show that cortical F-actin flows, driven by the depletion of myosin-2 at the
cell contact center, mediate the dynamic reorganization of adhesion receptors
and cell cortex at the contact. E-cadherin-dependent downregulation of the small
GTPase RhoA at the forming contact leads to both a depletion of myosin-2 and a
decrease of F-actin at the contact center. At the contact rim, in contrast, myosin-2
becomes enriched by the retraction of bleb-like protrusions, resulting in a cortical
tension gradient from the contact rim to its center. This tension gradient, in
turn, triggers centrifugal F-actin flows, leading to further accumulation of F-actin
at the contact rim and the progressive redistribution of E-cadherin from the contact
center to the rim. Eventually, this combination of actomyosin downregulation and
flows at the contact determines the characteristic molecular organization, with
E-cadherin and F-actin accumulating at the contact rim, where they are needed
to mechanically link the contractile cortices of the adhering cells.
acknowledged_ssus:
- _id: Bio
- _id: PreCl
acknowledgement: "We are grateful to Edwin Munro for their feedback and help with
the single particle analysis. We thank members of the Heisenberg and Loose labs
for their help and feedback on the manuscript, notably Xin Tong for making the PCS2-mCherry-AHPH
plasmid. Finally, we thank the Aquatics and Imaging & Optics facilities of ISTA
for their continuous support, especially Yann Cesbron for assistance with the laser
cutter. This work was supported by an ERC\r\nAdvanced Grant (MECSPEC) to C.-P.H."
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Feyza N
full_name: Arslan, Feyza N
id: 49DA7910-F248-11E8-B48F-1D18A9856A87
last_name: Arslan
orcid: 0000-0001-5809-9566
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Martin
full_name: Loose, Martin
id: 462D4284-F248-11E8-B48F-1D18A9856A87
last_name: Loose
orcid: 0000-0001-7309-9724
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Arslan FN, Hannezo EB, Merrin J, Loose M, Heisenberg C-PJ. Adhesion-induced
cortical flows pattern E-cadherin-mediated cell contacts. Current Biology.
2024;34(1):171-182.e8. doi:10.1016/j.cub.2023.11.067
apa: Arslan, F. N., Hannezo, E. B., Merrin, J., Loose, M., & Heisenberg, C.-P.
J. (2024). Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts.
Current Biology. Elsevier. https://doi.org/10.1016/j.cub.2023.11.067
chicago: Arslan, Feyza N, Edouard B Hannezo, Jack Merrin, Martin Loose, and Carl-Philipp
J Heisenberg. “Adhesion-Induced Cortical Flows Pattern E-Cadherin-Mediated Cell
Contacts.” Current Biology. Elsevier, 2024. https://doi.org/10.1016/j.cub.2023.11.067.
ieee: F. N. Arslan, E. B. Hannezo, J. Merrin, M. Loose, and C.-P. J. Heisenberg,
“Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts,” Current
Biology, vol. 34, no. 1. Elsevier, p. 171–182.e8, 2024.
ista: Arslan FN, Hannezo EB, Merrin J, Loose M, Heisenberg C-PJ. 2024. Adhesion-induced
cortical flows pattern E-cadherin-mediated cell contacts. Current Biology. 34(1),
171–182.e8.
mla: Arslan, Feyza N., et al. “Adhesion-Induced Cortical Flows Pattern E-Cadherin-Mediated
Cell Contacts.” Current Biology, vol. 34, no. 1, Elsevier, 2024, p. 171–182.e8,
doi:10.1016/j.cub.2023.11.067.
short: F.N. Arslan, E.B. Hannezo, J. Merrin, M. Loose, C.-P.J. Heisenberg, Current
Biology 34 (2024) 171–182.e8.
date_created: 2024-01-14T23:00:56Z
date_published: 2024-01-08T00:00:00Z
date_updated: 2024-01-17T08:20:40Z
day: '08'
ddc:
- '570'
department:
- _id: CaHe
- _id: EdHa
- _id: MaLo
- _id: NanoFab
doi: 10.1016/j.cub.2023.11.067
ec_funded: 1
file:
- access_level: open_access
checksum: 51220b76d72a614208f84bdbfbaf9b72
content_type: application/pdf
creator: dernst
date_created: 2024-01-16T10:53:31Z
date_updated: 2024-01-16T10:53:31Z
file_id: '14813'
file_name: 2024_CurrentBiology_Arslan.pdf
file_size: 5183861
relation: main_file
success: 1
file_date_updated: 2024-01-16T10:53:31Z
has_accepted_license: '1'
intvolume: ' 34'
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: 171-182.e8
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742573'
name: Interaction and feedback between cell mechanics and fate specification in
vertebrate gastrulation
publication: Current Biology
publication_identifier:
eissn:
- 1879-0445
issn:
- 0960-9822
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 34
year: '2024'
...
---
_id: '15024'
abstract:
- lang: eng
text: Electrostatic correlations between ions dissolved in water are known to impact
their transport properties in numerous ways, from conductivity to ion selectivity.
The effects of these correlations on the solvent itself remain, however, much
less clear. In particular, the addition of salt has been consistently reported
to affect the solution’s viscosity, but most modeling attempts fail to reproduce
experimental data even at moderate salt concentrations. Here, we use an approach
based on stochastic density functional theory, which accurately captures charge
fluctuations and correlations. We derive a simple analytical expression for the
viscosity correction in concentrated electrolytes, by directly linking it to the
liquid’s structure factor. Our prediction compares quantitatively to experimental
data at all temperatures and all salt concentrations up to the saturation limit.
This universal link between the microscopic structure and viscosity allows us
to shed light on the nanoscale dynamics of water and ions under highly concentrated
and correlated conditions.
acknowledgement: The author thanks Lydéric Bocquet, Baptiste Coquinot, and Mathieu
Lizée for fruitful discussions. This project received funding from the European
Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie
Grant Agreement No. 101034413.
article_number: '064503'
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Paul
full_name: Robin, Paul
id: 48c58128-57b0-11ee-9095-dc28fd97fc1d
last_name: Robin
orcid: 0000-0002-5728-9189
citation:
ama: Robin P. Correlation-induced viscous dissipation in concentrated electrolytes.
Journal of Chemical Physics. 2024;160(6). doi:10.1063/5.0188215
apa: Robin, P. (2024). Correlation-induced viscous dissipation in concentrated electrolytes.
Journal of Chemical Physics. AIP Publishing. https://doi.org/10.1063/5.0188215
chicago: Robin, Paul. “Correlation-Induced Viscous Dissipation in Concentrated Electrolytes.”
Journal of Chemical Physics. AIP Publishing, 2024. https://doi.org/10.1063/5.0188215.
ieee: P. Robin, “Correlation-induced viscous dissipation in concentrated electrolytes,”
Journal of Chemical Physics, vol. 160, no. 6. AIP Publishing, 2024.
ista: Robin P. 2024. Correlation-induced viscous dissipation in concentrated electrolytes.
Journal of Chemical Physics. 160(6), 064503.
mla: Robin, Paul. “Correlation-Induced Viscous Dissipation in Concentrated Electrolytes.”
Journal of Chemical Physics, vol. 160, no. 6, 064503, AIP Publishing, 2024,
doi:10.1063/5.0188215.
short: P. Robin, Journal of Chemical Physics 160 (2024).
date_created: 2024-02-25T23:00:55Z
date_published: 2024-02-14T00:00:00Z
date_updated: 2024-02-27T08:16:06Z
day: '14'
ddc:
- '540'
department:
- _id: EdHa
doi: 10.1063/5.0188215
ec_funded: 1
external_id:
arxiv:
- '2311.11784'
pmid:
- '38349632'
file:
- access_level: open_access
checksum: 0a5e0ae70849bce674466fc054390ec0
content_type: application/pdf
creator: dernst
date_created: 2024-02-27T08:12:52Z
date_updated: 2024-02-27T08:12:52Z
file_id: '15034'
file_name: 2024_JourChemicalPhysics_Robin.pdf
file_size: 5452738
relation: main_file
success: 1
file_date_updated: 2024-02-27T08:12:52Z
has_accepted_license: '1'
intvolume: ' 160'
issue: '6'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: fc2ed2f7-9c52-11eb-aca3-c01059dda49c
call_identifier: H2020
grant_number: '101034413'
name: 'IST-BRIDGE: International postdoctoral program'
publication: Journal of Chemical Physics
publication_identifier:
eissn:
- 1089-7690
issn:
- 0021-9606
publication_status: published
publisher: AIP Publishing
quality_controlled: '1'
scopus_import: '1'
status: public
title: Correlation-induced viscous dissipation in concentrated electrolytes
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 160
year: '2024'
...
---
_id: '12428'
abstract:
- lang: eng
text: The mammary gland consists of a bilayered epithelial structure with an extensively
branched morphology. The majority of this epithelial tree is laid down during
puberty, during which actively proliferating terminal end buds repeatedly elongate
and bifurcate to form the basic structure of the ductal tree. Mammary ducts consist
of a basal and luminal cell layer with a multitude of identified sub-lineages
within both layers. The understanding of how these different cell lineages are
cooperatively driving branching morphogenesis is a problem of crossing multiple
scales, as this requires information on the macroscopic branched structure of
the gland, as well as data on single-cell dynamics driving the morphogenic program.
Here we describe a method to combine genetic lineage tracing with whole-gland
branching analysis. Quantitative data on the global organ structure can be used
to derive a model for mammary gland branching morphogenesis and provide a backbone
on which the dynamics of individual cell lineages can be simulated and compared
to lineage-tracing approaches. Eventually, these quantitative models and experiments
allow to understand the couplings between the macroscopic shape of the mammary
gland and the underlying single-cell dynamics driving branching morphogenesis.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Colinda L.G.J.
full_name: Scheele, Colinda L.G.J.
last_name: Scheele
citation:
ama: 'Hannezo EB, Scheele CLGJ. A Guide Toward Multi-scale and Quantitative Branching
Analysis in the Mammary Gland. In: Margadant C, ed. Cell Migration in Three
Dimensions. Vol 2608. MIMB. Springer Nature; 2023:183-205. doi:10.1007/978-1-0716-2887-4_12'
apa: Hannezo, E. B., & Scheele, C. L. G. J. (2023). A Guide Toward Multi-scale
and Quantitative Branching Analysis in the Mammary Gland. In C. Margadant (Ed.),
Cell Migration in Three Dimensions (Vol. 2608, pp. 183–205). Springer Nature.
https://doi.org/10.1007/978-1-0716-2887-4_12
chicago: Hannezo, Edouard B, and Colinda L.G.J. Scheele. “A Guide Toward Multi-Scale
and Quantitative Branching Analysis in the Mammary Gland.” In Cell Migration
in Three Dimensions, edited by Coert Margadant, 2608:183–205. MIMB. Springer
Nature, 2023. https://doi.org/10.1007/978-1-0716-2887-4_12.
ieee: E. B. Hannezo and C. L. G. J. Scheele, “A Guide Toward Multi-scale and Quantitative
Branching Analysis in the Mammary Gland,” in Cell Migration in Three Dimensions,
vol. 2608, C. Margadant, Ed. Springer Nature, 2023, pp. 183–205.
ista: 'Hannezo EB, Scheele CLGJ. 2023.A Guide Toward Multi-scale and Quantitative
Branching Analysis in the Mammary Gland. In: Cell Migration in Three Dimensions.
Methods in Molecular Biology, vol. 2608, 183–205.'
mla: Hannezo, Edouard B., and Colinda L. G. J. Scheele. “A Guide Toward Multi-Scale
and Quantitative Branching Analysis in the Mammary Gland.” Cell Migration in
Three Dimensions, edited by Coert Margadant, vol. 2608, Springer Nature, 2023,
pp. 183–205, doi:10.1007/978-1-0716-2887-4_12.
short: E.B. Hannezo, C.L.G.J. Scheele, in:, C. Margadant (Ed.), Cell Migration in
Three Dimensions, Springer Nature, 2023, pp. 183–205.
date_created: 2023-01-29T23:00:58Z
date_published: 2023-01-19T00:00:00Z
date_updated: 2023-02-03T10:58:56Z
day: '19'
ddc:
- '570'
department:
- _id: EdHa
doi: 10.1007/978-1-0716-2887-4_12
editor:
- first_name: Coert
full_name: Margadant, Coert
last_name: Margadant
external_id:
pmid:
- '36653709'
file:
- access_level: open_access
checksum: aec1b8d3ba938ddf9d8fcb777f3c38ee
content_type: application/pdf
creator: dernst
date_created: 2023-02-03T10:56:39Z
date_updated: 2023-02-03T10:56:39Z
file_id: '12500'
file_name: 2023_MIMB_Hannezo.pdf
file_size: 826598
relation: main_file
success: 1
file_date_updated: 2023-02-03T10:56:39Z
has_accepted_license: '1'
intvolume: ' 2608'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: 183-205
pmid: 1
publication: Cell Migration in Three Dimensions
publication_identifier:
eisbn:
- '9781071628874'
eissn:
- 1940-6029
isbn:
- '9781071628867'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
series_title: MIMB
status: public
title: A Guide Toward Multi-scale and Quantitative Branching Analysis in the Mammary
Gland
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2608
year: '2023'
...
---
_id: '12818'
abstract:
- lang: eng
text: The multicellular organization of diverse systems, including embryos, intestines,
and tumors relies on coordinated cell migration in curved environments. In these
settings, cells establish supracellular patterns of motion, including collective
rotation and invasion. While such collective modes have been studied extensively
in flat systems, the consequences of geometrical and topological constraints on
collective migration in curved systems are largely unknown. Here, we discover
a collective mode of cell migration in rotating spherical tissues manifesting
as a propagating single-wavelength velocity wave. This wave is accompanied by
an apparently incompressible supracellular flow pattern featuring topological
defects as dictated by the spherical topology. Using a minimal active particle
model, we reveal that this collective mode arises from the effect of curvature
on the active flocking behavior of a cell layer confined to a spherical surface.
Our results thus identify curvature-induced velocity waves as a mode of collective
cell migration, impacting the dynamical organization of 3D curved tissues.
acknowledgement: We thank H. Abbaszadeh, M.J. Bowick, G. Gradziuk, M.C. Marchetti,
and S. Shankar for their helpful discussions. Funded by the Deutsche Forschungsgemeinschaft
(DFG, German Research Foundation)—Project-ID 201269156-SFB 1032 (Project B12). D.B.B.
is a NOMIS fellow supported by the NOMIS foundation and was in part supported by
a DFG fellowship within the Graduate School of Quantitative Biosciences Munich (QBM)
and Joachim Herz Stiftung. R.A. acknowledges support from the Human Frontier Science
Program (LT000475/2018-C) and from the National Science Foundation, through the
Center for the Physics of Biological Function (PHY-1734030). M.G. acknowledges support
from NIH R01GM140108 and Alfred Sloan Foundation. Funded by the Deutsche Forschungsgemeinschaft
(DFG, German Research Foundation)—Project-ID 201269156-SFB 1032 (Project B12).Open
Access funding enabled and organized by Projekt DEAL.
article_number: '1643'
article_processing_charge: No
article_type: original
author:
- first_name: Tom
full_name: Brandstätter, Tom
last_name: Brandstätter
- first_name: David
full_name: Brückner, David
id: e1e86031-6537-11eb-953a-f7ab92be508d
last_name: Brückner
orcid: 0000-0001-7205-2975
- first_name: Yu Long
full_name: Han, Yu Long
last_name: Han
- first_name: Ricard
full_name: Alert, Ricard
last_name: Alert
- first_name: Ming
full_name: Guo, Ming
last_name: Guo
- first_name: Chase P.
full_name: Broedersz, Chase P.
last_name: Broedersz
citation:
ama: Brandstätter T, Brückner D, Han YL, Alert R, Guo M, Broedersz CP. Curvature
induces active velocity waves in rotating spherical tissues. Nature Communications.
2023;14. doi:10.1038/s41467-023-37054-2
apa: Brandstätter, T., Brückner, D., Han, Y. L., Alert, R., Guo, M., & Broedersz,
C. P. (2023). Curvature induces active velocity waves in rotating spherical tissues.
Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-023-37054-2
chicago: Brandstätter, Tom, David Brückner, Yu Long Han, Ricard Alert, Ming Guo,
and Chase P. Broedersz. “Curvature Induces Active Velocity Waves in Rotating Spherical
Tissues.” Nature Communications. Springer Nature, 2023. https://doi.org/10.1038/s41467-023-37054-2.
ieee: T. Brandstätter, D. Brückner, Y. L. Han, R. Alert, M. Guo, and C. P. Broedersz,
“Curvature induces active velocity waves in rotating spherical tissues,” Nature
Communications, vol. 14. Springer Nature, 2023.
ista: Brandstätter T, Brückner D, Han YL, Alert R, Guo M, Broedersz CP. 2023. Curvature
induces active velocity waves in rotating spherical tissues. Nature Communications.
14, 1643.
mla: Brandstätter, Tom, et al. “Curvature Induces Active Velocity Waves in Rotating
Spherical Tissues.” Nature Communications, vol. 14, 1643, Springer Nature,
2023, doi:10.1038/s41467-023-37054-2.
short: T. Brandstätter, D. Brückner, Y.L. Han, R. Alert, M. Guo, C.P. Broedersz,
Nature Communications 14 (2023).
date_created: 2023-04-09T22:01:00Z
date_published: 2023-03-24T00:00:00Z
date_updated: 2023-08-01T14:05:30Z
day: '24'
ddc:
- '570'
department:
- _id: EdHa
doi: 10.1038/s41467-023-37054-2
external_id:
isi:
- '000959887700008'
pmid:
- '36964141'
file:
- access_level: open_access
checksum: 54f06f9eee11d43bab253f3492c983ba
content_type: application/pdf
creator: dernst
date_created: 2023-04-11T06:27:00Z
date_updated: 2023-04-11T06:27:00Z
file_id: '12821'
file_name: 2023_NatureComm_Brandstaetter.pdf
file_size: 4146777
relation: main_file
success: 1
file_date_updated: 2023-04-11T06:27:00Z
has_accepted_license: '1'
intvolume: ' 14'
isi: 1
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
publication: Nature Communications
publication_identifier:
eissn:
- 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Curvature induces active velocity waves in rotating spherical tissues
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 14
year: '2023'
...
---
_id: '12964'
abstract:
- lang: eng
text: "Pattern formation is of great importance for its contribution across different
biological behaviours. During developmental processes for example, patterns of
chemical gradients are\r\nestablished to determine cell fate and complex tissue
patterns emerge to define structures such\r\nas limbs and vascular networks. Patterns
are also seen in collectively migrating groups, for\r\ninstance traveling waves
of density emerging in moving animal flocks as well as collectively migrating
cells and tissues. To what extent these biological patterns arise spontaneously
through\r\nthe local interaction of individual constituents or are dictated by
higher level instructions is\r\nstill an open question however there is evidence
for the involvement of both types of process.\r\nWhere patterns arise spontaneously
there is a long standing interest in how far the interplay\r\nof mechanics, e.g.
force generation and deformation, and chemistry, e.g. gene regulation\r\nand signaling,
contributes to the behaviour. This is because many systems are able to both\r\nchemically
regulate mechanical force production and chemically sense mechanical deformation,\r\nforming
mechano-chemical feedback loops which can potentially become unstable towards\r\nspatio
and/or temporal patterning.\r\nWe work with experimental collaborators to investigate
the possibility that this type of\r\ninteraction drives pattern formation in biological
systems at different scales. We focus first on\r\ntissue-level ERK-density waves
observed during the wound healing response across different\r\nsystems where many
previous studies have proposed that patterns depend on polarized cell\r\nmigration
and arise from a mechanical flocking-like mechanism. By combining theory with\r\nmechanical
and optogenetic perturbation experiments on in vitro monolayers we instead find\r\nevidence
for mechanochemical pattern formation involving only scalar bilateral feedbacks\r\nbetween
ERK signaling and cell contraction. We perform further modeling and experiment\r\nto
study how this instability couples with polar cell migration in order to produce
a robust\r\nand efficient wound healing response. In a following chapter we implement
ERK-density\r\ncoupling and cell migration in a 2D active vertex model to investigate
the interaction of\r\nERK-density patterning with different tissue rheologies
and find that the spatio-temporal\r\ndynamics are able to both locally and globally
fluidize a tissue across the solid-fluid glass\r\ntransition. In a last chapter
we move towards lower spatial scales in the context of subcellular\r\npatterning
of the cell cytoskeleton where we investigate the transition between phases of\r\nspatially
homogeneous temporal oscillations and chaotic spatio-temporal patterning in the\r\ndynamics
of myosin and ROCK activities (a motor component of the actomyosin cytoskeleton\r\nand
its activator). Experimental evidence supports an intrinsic chemical oscillator
which we\r\nencode in a reaction model and couple to a contractile active gel
description of the cell cortex.\r\nThe model exhibits phases of chemical oscillations
and contractile spatial patterning which\r\nreproduce many features of the dynamics
seen in Drosophila oocyte epithelia in vivo. However,\r\nadditional pharmacological
perturbations to inhibit myosin contractility leaves the role of\r\ncontractile
instability unclear. We discuss alternative hypotheses and investigate the possibility\r\nof
reaction-diffusion instability."
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Daniel R
full_name: Boocock, Daniel R
id: 453AF628-F248-11E8-B48F-1D18A9856A87
last_name: Boocock
orcid: 0000-0002-1585-2631
citation:
ama: Boocock DR. Mechanochemical pattern formation across biological scales. 2023.
doi:10.15479/at:ista:12964
apa: Boocock, D. R. (2023). Mechanochemical pattern formation across biological
scales. Institute of Science and Technology Austria. https://doi.org/10.15479/at:ista:12964
chicago: Boocock, Daniel R. “Mechanochemical Pattern Formation across Biological
Scales.” Institute of Science and Technology Austria, 2023. https://doi.org/10.15479/at:ista:12964.
ieee: D. R. Boocock, “Mechanochemical pattern formation across biological scales,”
Institute of Science and Technology Austria, 2023.
ista: Boocock DR. 2023. Mechanochemical pattern formation across biological scales.
Institute of Science and Technology Austria.
mla: Boocock, Daniel R. Mechanochemical Pattern Formation across Biological Scales.
Institute of Science and Technology Austria, 2023, doi:10.15479/at:ista:12964.
short: D.R. Boocock, Mechanochemical Pattern Formation across Biological Scales,
Institute of Science and Technology Austria, 2023.
date_created: 2023-05-15T14:52:36Z
date_published: 2023-05-17T00:00:00Z
date_updated: 2023-08-04T11:02:40Z
day: '17'
ddc:
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department:
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ec_funded: 1
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creator: dboocock
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date_updated: 2023-05-19T07:04:25Z
embargo: 2024-05-17
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creator: dboocock
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language:
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oa_version: Published Version
page: '146'
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
publication_identifier:
isbn:
- 978-3-99078-032-9
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '8602'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
title: Mechanochemical pattern formation across biological scales
tmp:
image: /images/cc_by_nc_sa.png
legal_code_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC
BY-NC-SA 4.0)
short: CC BY-NC-SA (4.0)
type: dissertation
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
year: '2023'
...
---
_id: '14277'
abstract:
- lang: eng
text: Living tissues are characterized by an intrinsically mechanochemical interplay
of active physical forces and complex biochemical signaling pathways. Either feature
alone can give rise to complex emergent phenomena, for example, mechanically driven
glassy dynamics and rigidity transitions, or chemically driven reaction-diffusion
instabilities. An important question is how to quantitatively assess the contribution
of these different cues to the large-scale dynamics of biological materials. We
address this in Madin-Darby canine kidney (MDCK) monolayers, considering both
mechanochemical feedback between extracellular signal-regulated kinase (ERK) signaling
activity and cellular density as well as a mechanically active tissue rheology
via a self-propelled vertex model. We show that the relative strength of active
migration forces to mechanochemical couplings controls a transition from a uniform
active glass to periodic spatiotemporal waves. We parametrize the model from published
experimental data sets on MDCK monolayers and use it to make new predictions on
the correlation functions of cellular dynamics and the dynamics of topological
defects associated with the oscillatory phase of cells. Interestingly, MDCK monolayers
are best described by an intermediary parameter region in which both mechanochemical
couplings and noisy active propulsion have a strong influence on the dynamics.
Finally, we study how tissue rheology and ERK waves produce feedback on one another
and uncover a mechanism via which tissue fluidity can be controlled by mechanochemical
waves at both the local and global levels.
acknowledgement: We thank all members of the Hannezo group for discussions and suggestions,
as well as Sound Wai Phow for technical assistance. This work received funding from
the European Research Council under the EU Horizon 2020 research and innovation
program Grant Agreement No. 851288 (E.H.), JSPS KAKENHI Grant No. 21H05290, and
the Ministry of Education under the Research Centres of Excellence program through
the MBI at NUS.
article_number: '013001'
article_processing_charge: Yes
article_type: original
author:
- first_name: Daniel R
full_name: Boocock, Daniel R
id: 453AF628-F248-11E8-B48F-1D18A9856A87
last_name: Boocock
orcid: 0000-0002-1585-2631
- first_name: Tsuyoshi
full_name: Hirashima, Tsuyoshi
last_name: Hirashima
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
citation:
ama: Boocock DR, Hirashima T, Hannezo EB. Interplay between mechanochemical patterning
and glassy dynamics in cellular monolayers. PRX Life. 2023;1(1). doi:10.1103/prxlife.1.013001
apa: Boocock, D. R., Hirashima, T., & Hannezo, E. B. (2023). Interplay between
mechanochemical patterning and glassy dynamics in cellular monolayers. PRX
Life. American Physical Society. https://doi.org/10.1103/prxlife.1.013001
chicago: Boocock, Daniel R, Tsuyoshi Hirashima, and Edouard B Hannezo. “Interplay
between Mechanochemical Patterning and Glassy Dynamics in Cellular Monolayers.”
PRX Life. American Physical Society, 2023. https://doi.org/10.1103/prxlife.1.013001.
ieee: D. R. Boocock, T. Hirashima, and E. B. Hannezo, “Interplay between mechanochemical
patterning and glassy dynamics in cellular monolayers,” PRX Life, vol.
1, no. 1. American Physical Society, 2023.
ista: Boocock DR, Hirashima T, Hannezo EB. 2023. Interplay between mechanochemical
patterning and glassy dynamics in cellular monolayers. PRX Life. 1(1), 013001.
mla: Boocock, Daniel R., et al. “Interplay between Mechanochemical Patterning and
Glassy Dynamics in Cellular Monolayers.” PRX Life, vol. 1, no. 1, 013001,
American Physical Society, 2023, doi:10.1103/prxlife.1.013001.
short: D.R. Boocock, T. Hirashima, E.B. Hannezo, PRX Life 1 (2023).
date_created: 2023-09-06T08:30:59Z
date_published: 2023-07-20T00:00:00Z
date_updated: 2023-09-15T06:39:17Z
day: '20'
ddc:
- '570'
department:
- _id: EdHa
doi: 10.1103/prxlife.1.013001
ec_funded: 1
file:
- access_level: open_access
checksum: f881d98c89eb9f1aa136d7b781511553
content_type: application/pdf
creator: dernst
date_created: 2023-09-15T06:30:50Z
date_updated: 2023-09-15T06:30:50Z
file_id: '14335'
file_name: 2023_PRXLife_Boocock.pdf
file_size: 2559520
relation: main_file
success: 1
file_date_updated: 2023-09-15T06:30:50Z
has_accepted_license: '1'
intvolume: ' 1'
issue: '1'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
project:
- _id: 05943252-7A3F-11EA-A408-12923DDC885E
call_identifier: H2020
grant_number: '851288'
name: Design Principles of Branching Morphogenesis
publication: PRX Life
publication_identifier:
issn:
- 2835-8279
publication_status: published
publisher: American Physical Society
quality_controlled: '1'
status: public
title: Interplay between mechanochemical patterning and glassy dynamics in cellular
monolayers
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 1
year: '2023'
...
---
_id: '12710'
abstract:
- lang: eng
text: Surface curvature both emerges from, and influences the behavior of, living
objects at length scales ranging from cell membranes to single cells to tissues
and organs. The relevance of surface curvature in biology is supported by numerous
experimental and theoretical investigations in recent years. In this review, first,
a brief introduction to the key ideas of surface curvature in the context of biological
systems is given and the challenges that arise when measuring surface curvature
are discussed. Giving an overview of the emergence of curvature in biological
systems, its significance at different length scales becomes apparent. On the
other hand, summarizing current findings also shows that both single cells and
entire cell sheets, tissues or organisms respond to curvature by modulating their
shape and their migration behavior. Finally, the interplay between the distribution
of morphogens or micro-organisms and the emergence of curvature across length
scales is addressed with examples demonstrating these key mechanistic principles
of morphogenesis. Overall, this review highlights that curved interfaces are not
merely a passive by-product of the chemical, biological, and mechanical processes
but that curvature acts also as a signal that co-determines these processes.
acknowledgement: B.S. and A.R. contributed equally to this work. A.P.G.C. and P.R.F.
acknowledge the funding from Fundação para a Ciência e Tecnologia (Portugal), through
IDMEC, under LAETA project UIDB/50022/2020. T.H.V.P. acknowledges the funding from
Fundação para a Ciência e Tecnologia (Portugal), through Ph.D. Grant 2020.04417.BD.
A.S. acknowledges that this work was partially supported by the ATTRACT Investigator
Grant (no. A17/MS/11572821/MBRACE, to A.S.) from the Luxembourg National Research
Fund. The author thanks Gerardo Ceada for his help in the graphical representations.
N.A.K. acknowledges support from the European Research Council (grant 851960) and
the Gravitation Program “Materials Driven Regeneration,” funded by the Netherlands
Organization for Scientific Research (024.003.013). M.B.A. acknowledges support
from the French National Research Agency (grant ANR-201-8-CE1-3-0008 for the project
“Epimorph”). G.E.S.T. acknowledges funding by the Australian Research Council through
project DP200102593. A.C. acknowledges the funding from the Deutsche Forschungsgemeinschaft
(DFG) Emmy Noether Grant CI 203/-2 1, the Spanish Ministry of Science and Innovation
(PID2021-123013O-BI00) and the IKERBASQUE Basque Foundation for Science.
article_number: '2206110'
article_processing_charge: No
article_type: review
author:
- first_name: Barbara
full_name: Schamberger, Barbara
last_name: Schamberger
- first_name: Ricardo
full_name: Ziege, Ricardo
last_name: Ziege
- first_name: Karine
full_name: Anselme, Karine
last_name: Anselme
- first_name: Martine
full_name: Ben Amar, Martine
last_name: Ben Amar
- first_name: Michał
full_name: Bykowski, Michał
last_name: Bykowski
- first_name: André P.G.
full_name: Castro, André P.G.
last_name: Castro
- first_name: Amaia
full_name: Cipitria, Amaia
last_name: Cipitria
- first_name: Rhoslyn A.
full_name: Coles, Rhoslyn A.
last_name: Coles
- first_name: Rumiana
full_name: Dimova, Rumiana
last_name: Dimova
- first_name: Michaela
full_name: Eder, Michaela
last_name: Eder
- first_name: Sebastian
full_name: Ehrig, Sebastian
last_name: Ehrig
- first_name: Luis M.
full_name: Escudero, Luis M.
last_name: Escudero
- first_name: Myfanwy E.
full_name: Evans, Myfanwy E.
last_name: Evans
- first_name: Paulo R.
full_name: Fernandes, Paulo R.
last_name: Fernandes
- first_name: Peter
full_name: Fratzl, Peter
last_name: Fratzl
- first_name: Liesbet
full_name: Geris, Liesbet
last_name: Geris
- first_name: Notburga
full_name: Gierlinger, Notburga
last_name: Gierlinger
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Aleš
full_name: Iglič, Aleš
last_name: Iglič
- first_name: Jacob J.K.
full_name: Kirkensgaard, Jacob J.K.
last_name: Kirkensgaard
- first_name: Philip
full_name: Kollmannsberger, Philip
last_name: Kollmannsberger
- first_name: Łucja
full_name: Kowalewska, Łucja
last_name: Kowalewska
- first_name: Nicholas A.
full_name: Kurniawan, Nicholas A.
last_name: Kurniawan
- first_name: Ioannis
full_name: Papantoniou, Ioannis
last_name: Papantoniou
- first_name: Laurent
full_name: Pieuchot, Laurent
last_name: Pieuchot
- first_name: Tiago H.V.
full_name: Pires, Tiago H.V.
last_name: Pires
- first_name: Lars D.
full_name: Renner, Lars D.
last_name: Renner
- first_name: Andrew O.
full_name: Sageman-Furnas, Andrew O.
last_name: Sageman-Furnas
- first_name: Gerd E.
full_name: Schröder-Turk, Gerd E.
last_name: Schröder-Turk
- first_name: Anupam
full_name: Sengupta, Anupam
last_name: Sengupta
- first_name: Vikas R.
full_name: Sharma, Vikas R.
last_name: Sharma
- first_name: Antonio
full_name: Tagua, Antonio
last_name: Tagua
- first_name: Caterina
full_name: Tomba, Caterina
last_name: Tomba
- first_name: Xavier
full_name: Trepat, Xavier
last_name: Trepat
- first_name: Sarah L.
full_name: Waters, Sarah L.
last_name: Waters
- first_name: Edwina F.
full_name: Yeo, Edwina F.
last_name: Yeo
- first_name: Andreas
full_name: Roschger, Andreas
last_name: Roschger
- first_name: Cécile M.
full_name: Bidan, Cécile M.
last_name: Bidan
- first_name: John W.C.
full_name: Dunlop, John W.C.
last_name: Dunlop
citation:
ama: 'Schamberger B, Ziege R, Anselme K, et al. Curvature in biological systems:
Its quantification, emergence, and implications across the scales. Advanced
Materials. 2023;35(13). doi:10.1002/adma.202206110'
apa: 'Schamberger, B., Ziege, R., Anselme, K., Ben Amar, M., Bykowski, M., Castro,
A. P. G., … Dunlop, J. W. C. (2023). Curvature in biological systems: Its quantification,
emergence, and implications across the scales. Advanced Materials. Wiley.
https://doi.org/10.1002/adma.202206110'
chicago: 'Schamberger, Barbara, Ricardo Ziege, Karine Anselme, Martine Ben Amar,
Michał Bykowski, André P.G. Castro, Amaia Cipitria, et al. “Curvature in Biological
Systems: Its Quantification, Emergence, and Implications across the Scales.” Advanced
Materials. Wiley, 2023. https://doi.org/10.1002/adma.202206110.'
ieee: 'B. Schamberger et al., “Curvature in biological systems: Its quantification,
emergence, and implications across the scales,” Advanced Materials, vol.
35, no. 13. Wiley, 2023.'
ista: 'Schamberger B, Ziege R, Anselme K, Ben Amar M, Bykowski M, Castro APG, Cipitria
A, Coles RA, Dimova R, Eder M, Ehrig S, Escudero LM, Evans ME, Fernandes PR, Fratzl
P, Geris L, Gierlinger N, Hannezo EB, Iglič A, Kirkensgaard JJK, Kollmannsberger
P, Kowalewska Ł, Kurniawan NA, Papantoniou I, Pieuchot L, Pires THV, Renner LD,
Sageman-Furnas AO, Schröder-Turk GE, Sengupta A, Sharma VR, Tagua A, Tomba C,
Trepat X, Waters SL, Yeo EF, Roschger A, Bidan CM, Dunlop JWC. 2023. Curvature
in biological systems: Its quantification, emergence, and implications across
the scales. Advanced Materials. 35(13), 2206110.'
mla: 'Schamberger, Barbara, et al. “Curvature in Biological Systems: Its Quantification,
Emergence, and Implications across the Scales.” Advanced Materials, vol.
35, no. 13, 2206110, Wiley, 2023, doi:10.1002/adma.202206110.'
short: B. Schamberger, R. Ziege, K. Anselme, M. Ben Amar, M. Bykowski, A.P.G. Castro,
A. Cipitria, R.A. Coles, R. Dimova, M. Eder, S. Ehrig, L.M. Escudero, M.E. Evans,
P.R. Fernandes, P. Fratzl, L. Geris, N. Gierlinger, E.B. Hannezo, A. Iglič, J.J.K.
Kirkensgaard, P. Kollmannsberger, Ł. Kowalewska, N.A. Kurniawan, I. Papantoniou,
L. Pieuchot, T.H.V. Pires, L.D. Renner, A.O. Sageman-Furnas, G.E. Schröder-Turk,
A. Sengupta, V.R. Sharma, A. Tagua, C. Tomba, X. Trepat, S.L. Waters, E.F. Yeo,
A. Roschger, C.M. Bidan, J.W.C. Dunlop, Advanced Materials 35 (2023).
date_created: 2023-03-05T23:01:06Z
date_published: 2023-03-29T00:00:00Z
date_updated: 2023-09-26T10:56:46Z
day: '29'
ddc:
- '570'
department:
- _id: EdHa
doi: 10.1002/adma.202206110
external_id:
isi:
- '000941068900001'
pmid:
- '36461812'
file:
- access_level: open_access
checksum: 5c04d68130e97a0ecd1ca27fbc15a246
content_type: application/pdf
creator: dernst
date_created: 2023-09-26T10:51:56Z
date_updated: 2023-09-26T10:51:56Z
file_id: '14373'
file_name: 2023_AdvancedMaterials_Schamberger.pdf
file_size: 2898063
relation: main_file
success: 1
file_date_updated: 2023-09-26T10:51:56Z
has_accepted_license: '1'
intvolume: ' 35'
isi: 1
issue: '13'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
publication: Advanced Materials
publication_identifier:
eissn:
- 1521-4095
issn:
- 0935-9648
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Curvature in biological systems: Its quantification, emergence, and implications
across the scales'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 35
year: '2023'
...
---
_id: '14279'
abstract:
- lang: eng
text: "The zip file includes source data used in the manuscript \"CCR7 acts as both
a sensor and a sink for CCL19 to coordinate collective leukocyte migration\",
as well as a representative Jupyter notebook to reproduce the main figures. Please
see the preprint on bioRxiv and the DOI link there to access the final published
version. Note the title change between the preprint and the published manuscript.\r\nA
sample script for particle-based simulations of collective chemotaxis by self-generated
gradients is also included (see Self-generated_chemotaxis_sample_script.ipynb)
to generate exemplary cell trajectories. A detailed description of the simulation
setup is provided in the supplementary information of the manuscipt."
article_processing_charge: No
author:
- first_name: Mehmet C
full_name: Ucar, Mehmet C
id: 50B2A802-6007-11E9-A42B-EB23E6697425
last_name: Ucar
orcid: 0000-0003-0506-4217
citation:
ama: Ucar MC. Source data for the manuscript “CCR7 acts as both a sensor and a sink
for CCL19 to coordinate collective leukocyte migration.” 2023. doi:10.5281/ZENODO.8133960
apa: Ucar, M. C. (2023). Source data for the manuscript “CCR7 acts as both a sensor
and a sink for CCL19 to coordinate collective leukocyte migration.” Zenodo. https://doi.org/10.5281/ZENODO.8133960
chicago: Ucar, Mehmet C. “Source Data for the Manuscript ‘CCR7 Acts as Both a Sensor
and a Sink for CCL19 to Coordinate Collective Leukocyte Migration.’” Zenodo, 2023.
https://doi.org/10.5281/ZENODO.8133960.
ieee: M. C. Ucar, “Source data for the manuscript ‘CCR7 acts as both a sensor and
a sink for CCL19 to coordinate collective leukocyte migration.’” Zenodo, 2023.
ista: Ucar MC. 2023. Source data for the manuscript ‘CCR7 acts as both a sensor
and a sink for CCL19 to coordinate collective leukocyte migration’, Zenodo, 10.5281/ZENODO.8133960.
mla: Ucar, Mehmet C. Source Data for the Manuscript “CCR7 Acts as Both a Sensor
and a Sink for CCL19 to Coordinate Collective Leukocyte Migration.” Zenodo,
2023, doi:10.5281/ZENODO.8133960.
short: M.C. Ucar, (2023).
date_created: 2023-09-06T08:39:25Z
date_published: 2023-07-11T00:00:00Z
date_updated: 2023-10-03T11:42:58Z
day: '11'
ddc:
- '570'
department:
- _id: EdHa
doi: 10.5281/ZENODO.8133960
has_accepted_license: '1'
main_file_link:
- open_access: '1'
url: https://doi.org/10.5281/zenodo.8133960
month: '07'
oa: 1
oa_version: Published Version
publisher: Zenodo
related_material:
record:
- id: '14274'
relation: used_in_publication
status: public
status: public
title: Source data for the manuscript "CCR7 acts as both a sensor and a sink for CCL19
to coordinate collective leukocyte migration"
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: research_data_reference
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2023'
...
---
_id: '12837'
abstract:
- lang: eng
text: As developing tissues grow in size and undergo morphogenetic changes, their
material properties may be altered. Such changes result from tension dynamics
at cell contacts or cellular jamming. Yet, in many cases, the cellular mechanisms
controlling the physical state of growing tissues are unclear. We found that at
early developmental stages, the epithelium in the developing mouse spinal cord
maintains both high junctional tension and high fluidity. This is achieved via
a mechanism in which interkinetic nuclear movements generate cell area dynamics
that drive extensive cell rearrangements. Over time, the cell proliferation rate
declines, effectively solidifying the tissue. Thus, unlike well-studied jamming
transitions, the solidification uncovered here resembles a glass transition that
depends on the dynamical stresses generated by proliferation and differentiation.
Our finding that the fluidity of developing epithelia is linked to interkinetic
nuclear movements and the dynamics of growth is likely to be relevant to multiple
developing tissues.
acknowledgement: 'We thank S. Hippenmeyer for the reagents and C. P. Heisenberg, J.
Briscoe and K. Page for comments on the manuscript. This work was supported by IST
Austria; the European Research Council under Horizon 2020 research and innovation
programme grant no. 680037 and Horizon Europe grant 101044579 (A.K.); Austrian Science
Fund (FWF): F78 (Stem Cell Modulation) (A.K.); ISTFELLOW postdoctoral program (A.S.);
Narodowe Centrum Nauki, Poland SONATA, 2017/26/D/NZ2/00454 (M.Z.); and the Polish
National Agency for Academic Exchange (M.Z.).'
article_processing_charge: No
article_type: original
author:
- first_name: Laura
full_name: Bocanegra, Laura
id: 4896F754-F248-11E8-B48F-1D18A9856A87
last_name: Bocanegra
- first_name: Amrita
full_name: Singh, Amrita
id: 76250f9f-3a21-11eb-9a80-a6180a0d7958
last_name: Singh
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Marcin P
full_name: Zagórski, Marcin P
id: 343DA0DC-F248-11E8-B48F-1D18A9856A87
last_name: Zagórski
orcid: 0000-0001-7896-7762
- first_name: Anna
full_name: Kicheva, Anna
id: 3959A2A0-F248-11E8-B48F-1D18A9856A87
last_name: Kicheva
orcid: 0000-0003-4509-4998
citation:
ama: Bocanegra L, Singh A, Hannezo EB, Zagórski MP, Kicheva A. Cell cycle dynamics
control fluidity of the developing mouse neuroepithelium. Nature Physics.
2023;19:1050-1058. doi:10.1038/s41567-023-01977-w
apa: Bocanegra, L., Singh, A., Hannezo, E. B., Zagórski, M. P., & Kicheva, A.
(2023). Cell cycle dynamics control fluidity of the developing mouse neuroepithelium.
Nature Physics. Springer Nature. https://doi.org/10.1038/s41567-023-01977-w
chicago: Bocanegra, Laura, Amrita Singh, Edouard B Hannezo, Marcin P Zagórski, and
Anna Kicheva. “Cell Cycle Dynamics Control Fluidity of the Developing Mouse Neuroepithelium.”
Nature Physics. Springer Nature, 2023. https://doi.org/10.1038/s41567-023-01977-w.
ieee: L. Bocanegra, A. Singh, E. B. Hannezo, M. P. Zagórski, and A. Kicheva, “Cell
cycle dynamics control fluidity of the developing mouse neuroepithelium,” Nature
Physics, vol. 19. Springer Nature, pp. 1050–1058, 2023.
ista: Bocanegra L, Singh A, Hannezo EB, Zagórski MP, Kicheva A. 2023. Cell cycle
dynamics control fluidity of the developing mouse neuroepithelium. Nature Physics.
19, 1050–1058.
mla: Bocanegra, Laura, et al. “Cell Cycle Dynamics Control Fluidity of the Developing
Mouse Neuroepithelium.” Nature Physics, vol. 19, Springer Nature, 2023,
pp. 1050–58, doi:10.1038/s41567-023-01977-w.
short: L. Bocanegra, A. Singh, E.B. Hannezo, M.P. Zagórski, A. Kicheva, Nature Physics
19 (2023) 1050–1058.
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title: Cell cycle dynamics control fluidity of the developing mouse neuroepithelium
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
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year: '2023'
...
---
_id: '14426'
abstract:
- lang: eng
text: To meet the physiological demands of the body, organs need to establish a
functional tissue architecture and adequate size as the embryo develops to adulthood.
In the liver, uni- and bipotent progenitor differentiation into hepatocytes and
biliary epithelial cells (BECs), and their relative proportions, comprise the
functional architecture. Yet, the contribution of individual liver progenitors
at the organ level to both fates, and their specific proportion, is unresolved.
Combining mathematical modelling with organ-wide, multispectral FRaeppli-NLS lineage
tracing in zebrafish, we demonstrate that a precise BEC-to-hepatocyte ratio is
established (i) fast, (ii) solely by heterogeneous lineage decisions from uni-
and bipotent progenitors, and (iii) independent of subsequent cell type–specific
proliferation. Extending lineage tracing to adulthood determined that embryonic
cells undergo spatially heterogeneous three-dimensional growth associated with
distinct environments. Strikingly, giant clusters comprising almost half a ventral
lobe suggest lobe-specific dominant-like growth behaviours. We show substantial
hepatocyte polyploidy in juveniles representing another hallmark of postembryonic
liver growth. Our findings uncover heterogeneous progenitor contributions to tissue
architecture-defining cell type proportions and postembryonic organ growth as
key mechanisms forming the adult liver.
acknowledgement: "We thank the Ober group for discussion and comments on the manuscript.
We are grateful to\r\nDr. F. Lemaigre for feedback on the manuscript and Dr. T.
Piotrowski for invaluable support.\r\nWe thank the department of experimental medicine
(AEM) in Copenhagen for expert fish\r\ncare. We gratefully acknowledge the DanStem
Imaging Platform (University of Copenhagen)\r\nfor support and assistance in this
work.\r\nThis work is supported by Novo Nordisk Foundation grant NNF17CC0027852
(EAO);\r\nNordisk Foundation grant NNF19OC0058327 (EAO); Novo Nordisk Foundation
grant\r\nNNF17OC0031204 (PRL); https://novonordiskfonden.dk/en/; Danish National\r\nResearch
Foundation grant DNRF116 (EAO and AT); https://dg.dk/en/; John and Birthe Meyer\r\nFoundation
(PRL) and European Research Council (ERC) under the EU Horizon 2020 research and
Innovation Programme Grant Agreement No. 851288 (EH)."
article_number: e3002315
article_processing_charge: No
article_type: original
author:
- first_name: Iris A.
full_name: Unterweger, Iris A.
last_name: Unterweger
- first_name: Julie
full_name: Klepstad, Julie
last_name: Klepstad
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Pia R.
full_name: Lundegaard, Pia R.
last_name: Lundegaard
- first_name: Ala
full_name: Trusina, Ala
last_name: Trusina
- first_name: Elke A.
full_name: Ober, Elke A.
last_name: Ober
citation:
ama: Unterweger IA, Klepstad J, Hannezo EB, Lundegaard PR, Trusina A, Ober EA. Lineage
tracing identifies heterogeneous hepatoblast contribution to cell lineages and
postembryonic organ growth dynamics. PLoS Biology. 2023;21(10). doi:10.1371/journal.pbio.3002315
apa: Unterweger, I. A., Klepstad, J., Hannezo, E. B., Lundegaard, P. R., Trusina,
A., & Ober, E. A. (2023). Lineage tracing identifies heterogeneous hepatoblast
contribution to cell lineages and postembryonic organ growth dynamics. PLoS
Biology. Public Library of Science. https://doi.org/10.1371/journal.pbio.3002315
chicago: Unterweger, Iris A., Julie Klepstad, Edouard B Hannezo, Pia R. Lundegaard,
Ala Trusina, and Elke A. Ober. “Lineage Tracing Identifies Heterogeneous Hepatoblast
Contribution to Cell Lineages and Postembryonic Organ Growth Dynamics.” PLoS
Biology. Public Library of Science, 2023. https://doi.org/10.1371/journal.pbio.3002315.
ieee: I. A. Unterweger, J. Klepstad, E. B. Hannezo, P. R. Lundegaard, A. Trusina,
and E. A. Ober, “Lineage tracing identifies heterogeneous hepatoblast contribution
to cell lineages and postembryonic organ growth dynamics,” PLoS Biology,
vol. 21, no. 10. Public Library of Science, 2023.
ista: Unterweger IA, Klepstad J, Hannezo EB, Lundegaard PR, Trusina A, Ober EA.
2023. Lineage tracing identifies heterogeneous hepatoblast contribution to cell
lineages and postembryonic organ growth dynamics. PLoS Biology. 21(10), e3002315.
mla: Unterweger, Iris A., et al. “Lineage Tracing Identifies Heterogeneous Hepatoblast
Contribution to Cell Lineages and Postembryonic Organ Growth Dynamics.” PLoS
Biology, vol. 21, no. 10, e3002315, Public Library of Science, 2023, doi:10.1371/journal.pbio.3002315.
short: I.A. Unterweger, J. Klepstad, E.B. Hannezo, P.R. Lundegaard, A. Trusina,
E.A. Ober, PLoS Biology 21 (2023).
date_created: 2023-10-15T22:01:10Z
date_published: 2023-10-04T00:00:00Z
date_updated: 2023-10-16T07:25:48Z
day: '04'
ddc:
- '570'
department:
- _id: EdHa
doi: 10.1371/journal.pbio.3002315
ec_funded: 1
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content_type: application/pdf
creator: dernst
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oa_version: Published Version
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- _id: 05943252-7A3F-11EA-A408-12923DDC885E
call_identifier: H2020
grant_number: '851288'
name: Design Principles of Branching Morphogenesis
publication: PLoS Biology
publication_identifier:
eissn:
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publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
related_material:
link:
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url: https://github.com/JulieKlepstad/LiverDevelopment
scopus_import: '1'
status: public
title: Lineage tracing identifies heterogeneous hepatoblast contribution to cell lineages
and postembryonic organ growth dynamics
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 21
year: '2023'
...