---
_id: '10705'
abstract:
- lang: eng
text: Although rigidity and jamming transitions have been widely studied in physics
and material science, their importance in a number of biological processes, including
embryo development, tissue homeostasis, wound healing, and disease progression,
has only begun to be recognized in the past few years. The hypothesis that biological
systems can undergo rigidity/jamming transitions is attractive, as it would allow
these systems to change their material properties rapidly and strongly. However,
whether such transitions indeed occur in biological systems, how they are being
regulated, and what their physiological relevance might be, is still being debated.
Here, we review theoretical and experimental advances from the past few years,
focusing on the regulation and role of potential tissue rigidity transitions in
different biological processes.
acknowledgement: We thank present and former members of the Heisenberg and Hannezo
groups, in particular Bernat Corominas-Murtra and Nicoletta Petridou, for helpful
discussions, and Claudia Flandoli for the artwork. We apologize for not being able
to cite a number of highly relevant studies, to stay within the maximum allowed
number of citations.
article_processing_charge: No
article_type: original
author:
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Hannezo EB, Heisenberg C-PJ. Rigidity transitions in development and disease.
Trends in Cell Biology. 2022;32(5):P433-444. doi:10.1016/j.tcb.2021.12.006
apa: Hannezo, E. B., & Heisenberg, C.-P. J. (2022). Rigidity transitions in
development and disease. Trends in Cell Biology. Cell Press. https://doi.org/10.1016/j.tcb.2021.12.006
chicago: Hannezo, Edouard B, and Carl-Philipp J Heisenberg. “Rigidity Transitions
in Development and Disease.” Trends in Cell Biology. Cell Press, 2022.
https://doi.org/10.1016/j.tcb.2021.12.006.
ieee: E. B. Hannezo and C.-P. J. Heisenberg, “Rigidity transitions in development
and disease,” Trends in Cell Biology, vol. 32, no. 5. Cell Press, pp. P433-444,
2022.
ista: Hannezo EB, Heisenberg C-PJ. 2022. Rigidity transitions in development and
disease. Trends in Cell Biology. 32(5), P433-444.
mla: Hannezo, Edouard B., and Carl-Philipp J. Heisenberg. “Rigidity Transitions
in Development and Disease.” Trends in Cell Biology, vol. 32, no. 5, Cell
Press, 2022, pp. P433-444, doi:10.1016/j.tcb.2021.12.006.
short: E.B. Hannezo, C.-P.J. Heisenberg, Trends in Cell Biology 32 (2022) P433-444.
date_created: 2022-01-30T23:01:34Z
date_published: 2022-05-01T00:00:00Z
date_updated: 2023-08-02T14:03:53Z
day: '01'
department:
- _id: EdHa
- _id: CaHe
doi: 10.1016/j.tcb.2021.12.006
external_id:
isi:
- '000795773900009'
pmid:
- '35058104'
intvolume: ' 32'
isi: 1
issue: '5'
language:
- iso: eng
month: '05'
oa_version: None
page: P433-444
pmid: 1
publication: Trends in Cell Biology
publication_identifier:
eissn:
- 1879-3088
issn:
- 0962-8924
publication_status: published
publisher: Cell Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Rigidity transitions in development and disease
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 32
year: '2022'
...
---
_id: '10825'
abstract:
- lang: eng
text: In development, lineage segregation is coordinated in time and space. An important
example is the mammalian inner cell mass, in which the primitive endoderm (PrE,
founder of the yolk sac) physically segregates from the epiblast (EPI, founder
of the fetus). While the molecular requirements have been well studied, the physical
mechanisms determining spatial segregation between EPI and PrE remain elusive.
Here, we investigate the mechanical basis of EPI and PrE sorting. We find that
rather than the differences in static cell surface mechanical parameters as in
classical sorting models, it is the differences in surface fluctuations that robustly
ensure physical lineage sorting. These differential surface fluctuations systematically
correlate with differential cellular fluidity, which we propose together constitute
a non-equilibrium sorting mechanism for EPI and PrE lineages. By combining experiments
and modeling, we identify cell surface dynamics as a key factor orchestrating
the correct spatial segregation of the founder embryonic lineages.
acknowledgement: We are grateful to H. Niwa for Dox regulatable PB vector; G. Charras
for EzrinT567D cDNA; K. Jones for tdTomato ESCs, R26-Confetti ESCs, and laboratory
assistance; M. Kinoshita for pPB-CAG-H2B-BFP plasmid; P. Humphreys and D. Clements
for imaging support; G. Chu, P. Attlesey, and staff for animal husbandry; S. Pallett
for laboratory assistance; C. Mulas for critical feedback on the project; T. Boroviak
for single-cell RNA-seq; the EMBL Genomics Core Facility for sequencing; and M.
Merkel for developing and sharing the original version of the 3D Voronoi code. This
work was financially supported by BBSRC ( BB/Moo4023/1 and BB/T007044/1 to K.J.C.
and J.N., Alert16 grant BB/R000042 to E.K.P.), Leverhulme Trust ( RPG-2014-080 to
K.J.C. and J.N.), European Research Council ( 772798 -CellFateTech to K.J.C., 311637
-MorphoCorDiv and 820188 -NanoMechShape to E.K.P., Starting Grant 851288 to E.H.,
and 772426 -MeChemGui to K.F.), the Isaac Newton Trust (to E.K.P.), Medical Research
Council UK (MRC program award MC_UU_00012/5 to E.K.P.), the European Union’s Horizon
2020 research and innovation program under the Marie Sklodowska-Curie grant agreement
no. 641639 ( ITN Biopol , H.D.B. and E.K.P.), the Alexander von Humboldt Foundation
(Alexander von Humboldt Professorship to K.F.), EMBO ALTF 522-2021 (to P.S.), Centre
for Trophoblast Research (Next Generation fellowship to S.A.), and JSPS Overseas
Research Fellowships (to A.Y.). The Wellcome-MRC Cambridge Stem Cell Institute receives
core funding from Wellcome Trust ( 203151/Z/16/Z ) and MRC ( MC_PC_17230 ). For
the purpose of open access, the author has applied a CC BY public copyright licence
to any Author Accepted Manuscript version arising from this submission.
article_processing_charge: No
article_type: original
author:
- first_name: Ayaka
full_name: Yanagida, Ayaka
last_name: Yanagida
- first_name: Elena
full_name: Corujo-Simon, Elena
last_name: Corujo-Simon
- first_name: Christopher K.
full_name: Revell, Christopher K.
last_name: Revell
- first_name: Preeti
full_name: Sahu, Preeti
id: 55BA52EE-A185-11EA-88FD-18AD3DDC885E
last_name: Sahu
- first_name: Giuliano G.
full_name: Stirparo, Giuliano G.
last_name: Stirparo
- first_name: Irene M.
full_name: Aspalter, Irene M.
last_name: Aspalter
- first_name: Alex K.
full_name: Winkel, Alex K.
last_name: Winkel
- first_name: Ruby
full_name: Peters, Ruby
last_name: Peters
- first_name: Henry
full_name: De Belly, Henry
last_name: De Belly
- first_name: Davide A.D.
full_name: Cassani, Davide A.D.
last_name: Cassani
- first_name: Sarra
full_name: Achouri, Sarra
last_name: Achouri
- first_name: Raphael
full_name: Blumenfeld, Raphael
last_name: Blumenfeld
- first_name: Kristian
full_name: Franze, Kristian
last_name: Franze
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Ewa K.
full_name: Paluch, Ewa K.
last_name: Paluch
- first_name: Jennifer
full_name: Nichols, Jennifer
last_name: Nichols
- first_name: Kevin J.
full_name: Chalut, Kevin J.
last_name: Chalut
citation:
ama: Yanagida A, Corujo-Simon E, Revell CK, et al. Cell surface fluctuations regulate
early embryonic lineage sorting. Cell. 2022;185(5):777-793.e20. doi:10.1016/j.cell.2022.01.022
apa: Yanagida, A., Corujo-Simon, E., Revell, C. K., Sahu, P., Stirparo, G. G., Aspalter,
I. M., … Chalut, K. J. (2022). Cell surface fluctuations regulate early embryonic
lineage sorting. Cell. Cell Press. https://doi.org/10.1016/j.cell.2022.01.022
chicago: Yanagida, Ayaka, Elena Corujo-Simon, Christopher K. Revell, Preeti Sahu,
Giuliano G. Stirparo, Irene M. Aspalter, Alex K. Winkel, et al. “Cell Surface
Fluctuations Regulate Early Embryonic Lineage Sorting.” Cell. Cell Press,
2022. https://doi.org/10.1016/j.cell.2022.01.022.
ieee: A. Yanagida et al., “Cell surface fluctuations regulate early embryonic
lineage sorting,” Cell, vol. 185, no. 5. Cell Press, p. 777–793.e20, 2022.
ista: Yanagida A, Corujo-Simon E, Revell CK, Sahu P, Stirparo GG, Aspalter IM, Winkel
AK, Peters R, De Belly H, Cassani DAD, Achouri S, Blumenfeld R, Franze K, Hannezo
EB, Paluch EK, Nichols J, Chalut KJ. 2022. Cell surface fluctuations regulate
early embryonic lineage sorting. Cell. 185(5), 777–793.e20.
mla: Yanagida, Ayaka, et al. “Cell Surface Fluctuations Regulate Early Embryonic
Lineage Sorting.” Cell, vol. 185, no. 5, Cell Press, 2022, p. 777–793.e20,
doi:10.1016/j.cell.2022.01.022.
short: A. Yanagida, E. Corujo-Simon, C.K. Revell, P. Sahu, G.G. Stirparo, I.M. Aspalter,
A.K. Winkel, R. Peters, H. De Belly, D.A.D. Cassani, S. Achouri, R. Blumenfeld,
K. Franze, E.B. Hannezo, E.K. Paluch, J. Nichols, K.J. Chalut, Cell 185 (2022)
777–793.e20.
date_created: 2022-03-06T23:01:52Z
date_published: 2022-02-22T00:00:00Z
date_updated: 2023-08-02T14:43:50Z
day: '22'
ddc:
- '570'
department:
- _id: EdHa
doi: 10.1016/j.cell.2022.01.022
ec_funded: 1
external_id:
isi:
- '000796293700007'
pmid:
- '35196500'
file:
- access_level: open_access
checksum: ae305060e8031297771b89dae9e36a29
content_type: application/pdf
creator: dernst
date_created: 2022-03-07T07:55:23Z
date_updated: 2022-03-07T07:55:23Z
file_id: '10831'
file_name: 2022_Cell_Yanagida.pdf
file_size: 8478995
relation: main_file
success: 1
file_date_updated: 2022-03-07T07:55:23Z
has_accepted_license: '1'
intvolume: ' 185'
isi: 1
issue: '5'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '02'
oa: 1
oa_version: Published Version
page: 777-793.e20
pmid: 1
project:
- _id: 05943252-7A3F-11EA-A408-12923DDC885E
call_identifier: H2020
grant_number: '851288'
name: Design Principles of Branching Morphogenesis
publication: Cell
publication_identifier:
eissn:
- '10974172'
issn:
- '00928674'
publication_status: published
publisher: Cell Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Cell surface fluctuations regulate early embryonic lineage sorting
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 185
year: '2022'
...
---
_id: '12209'
abstract:
- lang: eng
text: Embryo development requires biochemical signalling to generate patterns of
cell fates and active mechanical forces to drive tissue shape changes. However,
how these processes are coordinated, and how tissue patterning is preserved despite
the cellular flows occurring during morphogenesis, remains poorly understood.
Gastrulation is a crucial embryonic stage that involves both patterning and internalization
of the mesendoderm germ layer tissue. Here we show that, in zebrafish embryos,
a gradient in Nodal signalling orchestrates pattern-preserving internalization
movements by triggering a motility-driven unjamming transition. In addition to
its role as a morphogen determining embryo patterning, graded Nodal signalling
mechanically subdivides the mesendoderm into a small fraction of highly protrusive
leader cells, able to autonomously internalize via local unjamming, and less protrusive
followers, which need to be pulled inwards by the leaders. The Nodal gradient
further enforces a code of preferential adhesion coupling leaders to their immediate
followers, resulting in a collective and ordered mode of internalization that
preserves mesendoderm patterning. Integrating this dual mechanical role of Nodal
signalling into minimal active particle simulations quantitatively predicts both
physiological and experimentally perturbed internalization movements. This provides
a quantitative framework for how a morphogen-encoded unjamming transition can
bidirectionally couple tissue mechanics with patterning during complex three-dimensional
morphogenesis.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: "We thank K. Sampath, A. Pauli and Y. Bellaїche for feedback on the
manuscript. We also thank the members of the Heisenberg group, in particular A.
Schauer and F. Nur Arslan, for help, technical advice and discussions, and the Bioimaging
and Life Science facilities at IST\r\nAustria for continuous support. We thank C.
Flandoli for the artwork in the figures. This work was supported by postdoctoral
fellowships from EMBO (LTF-850-2017) and HFSP (LT000429/2018-L2) to D.P. and the
European Union (European Research Council starting grant 851288 to É.H. and European
Research Council advanced grant 742573 to C.-P.H.)."
article_processing_charge: No
article_type: original
author:
- first_name: Diana C
full_name: Nunes Pinheiro, Diana C
id: 2E839F16-F248-11E8-B48F-1D18A9856A87
last_name: Nunes Pinheiro
orcid: 0000-0003-4333-7503
- first_name: Roland
full_name: Kardos, Roland
id: 4039350E-F248-11E8-B48F-1D18A9856A87
last_name: Kardos
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Nunes Pinheiro DC, Kardos R, Hannezo EB, Heisenberg C-PJ. Morphogen gradient
orchestrates pattern-preserving tissue morphogenesis via motility-driven unjamming.
Nature Physics. 2022;18(12):1482-1493. doi:10.1038/s41567-022-01787-6
apa: Nunes Pinheiro, D. C., Kardos, R., Hannezo, E. B., & Heisenberg, C.-P.
J. (2022). Morphogen gradient orchestrates pattern-preserving tissue morphogenesis
via motility-driven unjamming. Nature Physics. Springer Nature. https://doi.org/10.1038/s41567-022-01787-6
chicago: Nunes Pinheiro, Diana C, Roland Kardos, Edouard B Hannezo, and Carl-Philipp
J Heisenberg. “Morphogen Gradient Orchestrates Pattern-Preserving Tissue Morphogenesis
via Motility-Driven Unjamming.” Nature Physics. Springer Nature, 2022.
https://doi.org/10.1038/s41567-022-01787-6.
ieee: D. C. Nunes Pinheiro, R. Kardos, E. B. Hannezo, and C.-P. J. Heisenberg, “Morphogen
gradient orchestrates pattern-preserving tissue morphogenesis via motility-driven
unjamming,” Nature Physics, vol. 18, no. 12. Springer Nature, pp. 1482–1493,
2022.
ista: Nunes Pinheiro DC, Kardos R, Hannezo EB, Heisenberg C-PJ. 2022. Morphogen
gradient orchestrates pattern-preserving tissue morphogenesis via motility-driven
unjamming. Nature Physics. 18(12), 1482–1493.
mla: Nunes Pinheiro, Diana C., et al. “Morphogen Gradient Orchestrates Pattern-Preserving
Tissue Morphogenesis via Motility-Driven Unjamming.” Nature Physics, vol.
18, no. 12, Springer Nature, 2022, pp. 1482–93, doi:10.1038/s41567-022-01787-6.
short: D.C. Nunes Pinheiro, R. Kardos, E.B. Hannezo, C.-P.J. Heisenberg, Nature
Physics 18 (2022) 1482–1493.
date_created: 2023-01-16T09:45:19Z
date_published: 2022-12-01T00:00:00Z
date_updated: 2023-08-04T09:15:58Z
day: '01'
ddc:
- '570'
department:
- _id: CaHe
- _id: EdHa
doi: 10.1038/s41567-022-01787-6
ec_funded: 1
external_id:
isi:
- '000871319900002'
file:
- access_level: open_access
checksum: c86a8e8d80d1bfc46d56a01e88a2526a
content_type: application/pdf
creator: dernst
date_created: 2023-01-27T07:32:01Z
date_updated: 2023-01-27T07:32:01Z
file_id: '12412'
file_name: 2022_NaturePhysics_Pinheiro.pdf
file_size: 36703569
relation: main_file
success: 1
file_date_updated: 2023-01-27T07:32:01Z
has_accepted_license: '1'
intvolume: ' 18'
isi: 1
issue: '12'
keyword:
- General Physics and Astronomy
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
page: 1482-1493
project:
- _id: 26520D1E-B435-11E9-9278-68D0E5697425
grant_number: ALTF 850-2017
name: Coordination of mesendoderm cell fate specification and internalization during
zebrafish gastrulation
- _id: 26520D1E-B435-11E9-9278-68D0E5697425
grant_number: ALTF 850-2017
name: Coordination of mesendoderm cell fate specification and internalization during
zebrafish gastrulation
- _id: 05943252-7A3F-11EA-A408-12923DDC885E
call_identifier: H2020
grant_number: '851288'
name: Design Principles of Branching Morphogenesis
- _id: 260F1432-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742573'
name: Interaction and feedback between cell mechanics and fate specification in
vertebrate gastrulation
publication: Nature Physics
publication_identifier:
eissn:
- 1745-2481
issn:
- 1745-2473
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Morphogen gradient orchestrates pattern-preserving tissue morphogenesis via
motility-driven unjamming
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 18
year: '2022'
...
---
_id: '12217'
abstract:
- lang: eng
text: The development dynamics and self-organization of glandular branched epithelia
is of utmost importance for our understanding of diverse processes ranging from
normal tissue growth to the growth of cancerous tissues. Using single primary
murine pancreatic ductal adenocarcinoma (PDAC) cells embedded in a collagen matrix
and adapted media supplementation, we generate organoids that self-organize into
highly branched structures displaying a seamless lumen connecting terminal end
buds, replicating in vivo PDAC architecture. We identify distinct morphogenesis
phases, each characterized by a unique pattern of cell invasion, matrix deformation,
protein expression, and respective molecular dependencies. We propose a minimal
theoretical model of a branching and proliferating tissue, capturing the dynamics
of the first phases. Observing the interaction of morphogenesis, mechanical environment
and gene expression in vitro sets a benchmark for the understanding of self-organization
processes governing complex organoid structure formation processes and branching
morphogenesis.
acknowledgement: "A.R.B. acknowledges the financial support of the European Research
Council (ERC) through the funding of the grant Principles of Integrin Mechanics
and Adhesion (PoINT) and the German Research Foundation (DFG, SFB 1032, project
ID 201269156). E.H. was supported by the European Union (European Research Council
Starting Grant 851288). D.S., M.R., and R.R. acknowledge the support by the German
Research Foundation (DFG, SFB1321 Modeling and Targeting Pancreatic Cancer, Project
S01, project ID 329628492). C.S. and M.R. acknowledge the support by the German
Research Foundation (DFG, SFB1321 Modeling and Targeting Pancreatic Cancer, Project
12, project ID 329628492). M.R. was supported by the German Research Foundation
(DFG RE 3723/4-1). A.P. and M.R. were supported by the German Cancer Aid (Max-Eder
Program 111273 and 70114328).\r\nOpen Access funding enabled and organized by Projekt
DEAL."
article_number: '5219'
article_processing_charge: No
article_type: original
author:
- first_name: S.
full_name: Randriamanantsoa, S.
last_name: Randriamanantsoa
- first_name: A.
full_name: Papargyriou, A.
last_name: Papargyriou
- first_name: H. C.
full_name: Maurer, H. C.
last_name: Maurer
- first_name: K.
full_name: Peschke, K.
last_name: Peschke
- first_name: M.
full_name: Schuster, M.
last_name: Schuster
- first_name: G.
full_name: Zecchin, G.
last_name: Zecchin
- first_name: K.
full_name: Steiger, K.
last_name: Steiger
- first_name: R.
full_name: Öllinger, R.
last_name: Öllinger
- first_name: D.
full_name: Saur, D.
last_name: Saur
- first_name: C.
full_name: Scheel, C.
last_name: Scheel
- first_name: R.
full_name: Rad, R.
last_name: Rad
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: M.
full_name: Reichert, M.
last_name: Reichert
- first_name: A. R.
full_name: Bausch, A. R.
last_name: Bausch
citation:
ama: Randriamanantsoa S, Papargyriou A, Maurer HC, et al. Spatiotemporal dynamics
of self-organized branching in pancreas-derived organoids. Nature Communications.
2022;13. doi:10.1038/s41467-022-32806-y
apa: Randriamanantsoa, S., Papargyriou, A., Maurer, H. C., Peschke, K., Schuster,
M., Zecchin, G., … Bausch, A. R. (2022). Spatiotemporal dynamics of self-organized
branching in pancreas-derived organoids. Nature Communications. Springer
Nature. https://doi.org/10.1038/s41467-022-32806-y
chicago: Randriamanantsoa, S., A. Papargyriou, H. C. Maurer, K. Peschke, M. Schuster,
G. Zecchin, K. Steiger, et al. “Spatiotemporal Dynamics of Self-Organized Branching
in Pancreas-Derived Organoids.” Nature Communications. Springer Nature,
2022. https://doi.org/10.1038/s41467-022-32806-y.
ieee: S. Randriamanantsoa et al., “Spatiotemporal dynamics of self-organized
branching in pancreas-derived organoids,” Nature Communications, vol. 13.
Springer Nature, 2022.
ista: Randriamanantsoa S, Papargyriou A, Maurer HC, Peschke K, Schuster M, Zecchin
G, Steiger K, Öllinger R, Saur D, Scheel C, Rad R, Hannezo EB, Reichert M, Bausch
AR. 2022. Spatiotemporal dynamics of self-organized branching in pancreas-derived
organoids. Nature Communications. 13, 5219.
mla: Randriamanantsoa, S., et al. “Spatiotemporal Dynamics of Self-Organized Branching
in Pancreas-Derived Organoids.” Nature Communications, vol. 13, 5219, Springer
Nature, 2022, doi:10.1038/s41467-022-32806-y.
short: S. Randriamanantsoa, A. Papargyriou, H.C. Maurer, K. Peschke, M. Schuster,
G. Zecchin, K. Steiger, R. Öllinger, D. Saur, C. Scheel, R. Rad, E.B. Hannezo,
M. Reichert, A.R. Bausch, Nature Communications 13 (2022).
date_created: 2023-01-16T09:46:53Z
date_published: 2022-09-05T00:00:00Z
date_updated: 2023-08-04T09:25:23Z
day: '05'
ddc:
- '570'
department:
- _id: EdHa
doi: 10.1038/s41467-022-32806-y
ec_funded: 1
external_id:
isi:
- '000850348400025'
file:
- access_level: open_access
checksum: 295261b5172274fd5b8f85a6a6058828
content_type: application/pdf
creator: dernst
date_created: 2023-01-27T08:14:48Z
date_updated: 2023-01-27T08:14:48Z
file_id: '12416'
file_name: 2022_NatureCommunications_Randriamanantsoa.pdf
file_size: 22645149
relation: main_file
success: 1
file_date_updated: 2023-01-27T08:14:48Z
has_accepted_license: '1'
intvolume: ' 13'
isi: 1
keyword:
- General Physics and Astronomy
- General Biochemistry
- Genetics and Molecular Biology
- General Chemistry
- Multidisciplinary
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
project:
- _id: 05943252-7A3F-11EA-A408-12923DDC885E
call_identifier: H2020
grant_number: '851288'
name: Design Principles of Branching Morphogenesis
publication: Nature Communications
publication_identifier:
issn:
- 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
record:
- id: '13068'
relation: research_data
status: public
scopus_import: '1'
status: public
title: Spatiotemporal dynamics of self-organized branching in pancreas-derived organoids
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 13
year: '2022'
...
---
_id: '12253'
abstract:
- lang: eng
text: The sculpting of germ layers during gastrulation relies on the coordinated
migration of progenitor cells, yet the cues controlling these long-range directed
movements remain largely unknown. While directional migration often relies on
a chemokine gradient generated from a localized source, we find that zebrafish
ventrolateral mesoderm is guided by a self-generated gradient of the initially
uniformly expressed and secreted protein Toddler/ELABELA/Apela. We show that the
Apelin receptor, which is specifically expressed in mesodermal cells, has a dual
role during gastrulation, acting as a scavenger receptor to generate a Toddler
gradient, and as a chemokine receptor to sense this guidance cue. Thus, we uncover
a single receptor–based self-generated gradient as the enigmatic guidance cue
that can robustly steer the directional migration of mesoderm through the complex
and continuously changing environment of the gastrulating embryo.
acknowledgement: 'We thank K. Aumayer and the team of the biooptics facility at the
Vienna Biocenter, particularly P. Pasierbek and T. Müller, for support with microscopy;
K. Panser, C. Pribitzer, and the animal facility personnel for taking care of zebrafish;
M. Binner and A. Bandura for help with genotyping; M. Codina Tobias for help with
establishing the conditions for the Toddler overexpression compensation experiment;
T. Lubiana Alves for sharing the code for scRNA-Seq analyses; the Heisenberg laboratory,
particularly D. Pinheiro, for joint laboratory meetings, discussions on the project,
and providing the tg(gsc:CAAX-GFP) fish line; the Raz laboratory for providing the
Lifeact-GFP plasmid; A. Andersen, A. Schier, C.-P. Heisenberg, and E. Tanaka for
comments on the manuscript; and the entire Pauli laboratory, particularly K. Gert
and V. Deneke, for valuable discussions and feedback on the manuscript. Funding:
Work in A.P.’s laboratory has been supported by the IMP, which receives institutional
funding from Boehringer Ingelheim and the Austrian Research Promotion Agency (Headquarter
grant FFG-852936), as well as the FWF START program (Y 1031-B28 to A.P.), the Human
Frontier Science Program (HFSP) Career Development Award (CDA00066/2015 to A.P.)
and Young Investigator Grant (RGY0079/2020 to A.P.), the SFB RNA-Deco (project number
F 80 to A.P.), a Whitman Center Fellowship from the Marine Biological Laboratory
(to A.P.), and EMBO-YIP funds (to A.P.). This work was supported by the European
Union (European Research Council Starting Grant 851288 to E.H.). For the purpose
of Open Access, the authors have applied a CC BY public copyright license to any
Author Accepted Manuscript (AAM) version arising from this submission.'
article_number: eadd2488
article_processing_charge: No
article_type: original
author:
- first_name: Jessica
full_name: Stock, Jessica
last_name: Stock
- first_name: Tomas
full_name: Kazmar, Tomas
last_name: Kazmar
- first_name: Friederike
full_name: Schlumm, Friederike
last_name: Schlumm
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Andrea
full_name: Pauli, Andrea
last_name: Pauli
citation:
ama: Stock J, Kazmar T, Schlumm F, Hannezo EB, Pauli A. A self-generated Toddler
gradient guides mesodermal cell migration. Science Advances. 2022;8(37).
doi:10.1126/sciadv.add2488
apa: Stock, J., Kazmar, T., Schlumm, F., Hannezo, E. B., & Pauli, A. (2022).
A self-generated Toddler gradient guides mesodermal cell migration. Science
Advances. American Association for the Advancement of Science. https://doi.org/10.1126/sciadv.add2488
chicago: Stock, Jessica, Tomas Kazmar, Friederike Schlumm, Edouard B Hannezo, and
Andrea Pauli. “A Self-Generated Toddler Gradient Guides Mesodermal Cell Migration.”
Science Advances. American Association for the Advancement of Science,
2022. https://doi.org/10.1126/sciadv.add2488.
ieee: J. Stock, T. Kazmar, F. Schlumm, E. B. Hannezo, and A. Pauli, “A self-generated
Toddler gradient guides mesodermal cell migration,” Science Advances, vol.
8, no. 37. American Association for the Advancement of Science, 2022.
ista: Stock J, Kazmar T, Schlumm F, Hannezo EB, Pauli A. 2022. A self-generated
Toddler gradient guides mesodermal cell migration. Science Advances. 8(37), eadd2488.
mla: Stock, Jessica, et al. “A Self-Generated Toddler Gradient Guides Mesodermal
Cell Migration.” Science Advances, vol. 8, no. 37, eadd2488, American Association
for the Advancement of Science, 2022, doi:10.1126/sciadv.add2488.
short: J. Stock, T. Kazmar, F. Schlumm, E.B. Hannezo, A. Pauli, Science Advances
8 (2022).
date_created: 2023-01-16T09:57:10Z
date_published: 2022-09-14T00:00:00Z
date_updated: 2023-08-04T09:49:59Z
day: '14'
ddc:
- '570'
department:
- _id: EdHa
doi: 10.1126/sciadv.add2488
ec_funded: 1
external_id:
isi:
- '000888875000009'
pmid:
- '36103529'
file:
- access_level: open_access
checksum: f59cdb824e5d4221045def81f46f6c65
content_type: application/pdf
creator: dernst
date_created: 2023-01-30T09:27:49Z
date_updated: 2023-01-30T09:27:49Z
file_id: '12444'
file_name: 2022_ScienceAdvances_Stock.pdf
file_size: 1636732
relation: main_file
success: 1
file_date_updated: 2023-01-30T09:27:49Z
has_accepted_license: '1'
intvolume: ' 8'
isi: 1
issue: '37'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 05943252-7A3F-11EA-A408-12923DDC885E
call_identifier: H2020
grant_number: '851288'
name: Design Principles of Branching Morphogenesis
publication: Science Advances
publication_identifier:
issn:
- 2375-2548
publication_status: published
publisher: American Association for the Advancement of Science
quality_controlled: '1'
scopus_import: '1'
status: public
title: A self-generated Toddler gradient guides mesodermal cell migration
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 8
year: '2022'
...
---
_id: '12277'
abstract:
- lang: eng
text: Cell migration in confining physiological environments relies on the concerted
dynamics of several cellular components, including protrusions, adhesions with
the environment, and the cell nucleus. However, it remains poorly understood how
the dynamic interplay of these components and the cell polarity determine the
emergent migration behavior at the cellular scale. Here, we combine data-driven
inference with a mechanistic bottom-up approach to develop a model for protrusion
and polarity dynamics in confined cell migration, revealing how the cellular dynamics
adapt to confining geometries. Specifically, we use experimental data of joint
protrusion-nucleus migration trajectories of cells on confining micropatterns
to systematically determine a mechanistic model linking the stochastic dynamics
of cell polarity, protrusions, and nucleus. This model indicates that the cellular
dynamics adapt to confining constrictions through a switch in the polarity dynamics
from a negative to a positive self-reinforcing feedback loop. Our model further
reveals how this feedback loop leads to stereotypical cycles of protrusion-nucleus
dynamics that drive the migration of the cell through constrictions. These cycles
are disrupted upon perturbation of cytoskeletal components, indicating that the
positive feedback is controlled by cellular migration mechanisms. Our data-driven
theoretical approach therefore identifies polarity feedback adaptation as a key
mechanism in confined cell migration.
acknowledgement: "We thank Grzegorz Gradziuk, StevenRiedijk, Janni Harju, and M. R.
Schnucki for helpful discussions, and Andriy Goychuk for advice on the image segmentation.
This project\r\nwas funded by the Deutsche Forschungsgemeinschaft (DFG, German Research
Foundation), Project No. 201269156—SFB 1032 (Projects B01 and B12). D. B. B. is
supported by the NOMIS Foundation and in part by a DFG fellowship within the Graduate
School of Quantitative Biosciences Munich (QBM), as well as by the Joachim Herz
Stiftung."
article_number: '031041'
article_processing_charge: No
article_type: original
author:
- first_name: David
full_name: Brückner, David
id: e1e86031-6537-11eb-953a-f7ab92be508d
last_name: Brückner
orcid: 0000-0001-7205-2975
- first_name: Matthew
full_name: Schmitt, Matthew
last_name: Schmitt
- first_name: Alexandra
full_name: Fink, Alexandra
last_name: Fink
- first_name: Georg
full_name: Ladurner, Georg
last_name: Ladurner
- first_name: Johannes
full_name: Flommersfeld, Johannes
last_name: Flommersfeld
- first_name: Nicolas
full_name: Arlt, Nicolas
last_name: Arlt
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Joachim O.
full_name: Rädler, Joachim O.
last_name: Rädler
- first_name: Chase P.
full_name: Broedersz, Chase P.
last_name: Broedersz
citation:
ama: Brückner D, Schmitt M, Fink A, et al. Geometry adaptation of protrusion and
polarity dynamics in confined cell migration. Physical Review X. 2022;12(3).
doi:10.1103/physrevx.12.031041
apa: Brückner, D., Schmitt, M., Fink, A., Ladurner, G., Flommersfeld, J., Arlt,
N., … Broedersz, C. P. (2022). Geometry adaptation of protrusion and polarity
dynamics in confined cell migration. Physical Review X. American Physical
Society. https://doi.org/10.1103/physrevx.12.031041
chicago: Brückner, David, Matthew Schmitt, Alexandra Fink, Georg Ladurner, Johannes
Flommersfeld, Nicolas Arlt, Edouard B Hannezo, Joachim O. Rädler, and Chase P.
Broedersz. “Geometry Adaptation of Protrusion and Polarity Dynamics in Confined
Cell Migration.” Physical Review X. American Physical Society, 2022. https://doi.org/10.1103/physrevx.12.031041.
ieee: D. Brückner et al., “Geometry adaptation of protrusion and polarity
dynamics in confined cell migration,” Physical Review X, vol. 12, no. 3.
American Physical Society, 2022.
ista: Brückner D, Schmitt M, Fink A, Ladurner G, Flommersfeld J, Arlt N, Hannezo
EB, Rädler JO, Broedersz CP. 2022. Geometry adaptation of protrusion and polarity
dynamics in confined cell migration. Physical Review X. 12(3), 031041.
mla: Brückner, David, et al. “Geometry Adaptation of Protrusion and Polarity Dynamics
in Confined Cell Migration.” Physical Review X, vol. 12, no. 3, 031041,
American Physical Society, 2022, doi:10.1103/physrevx.12.031041.
short: D. Brückner, M. Schmitt, A. Fink, G. Ladurner, J. Flommersfeld, N. Arlt,
E.B. Hannezo, J.O. Rädler, C.P. Broedersz, Physical Review X 12 (2022).
date_created: 2023-01-16T10:02:06Z
date_published: 2022-09-20T00:00:00Z
date_updated: 2023-08-04T10:25:49Z
day: '20'
ddc:
- '530'
- '570'
department:
- _id: EdHa
doi: 10.1103/physrevx.12.031041
external_id:
arxiv:
- '2106.01014'
isi:
- '000861534700001'
file:
- access_level: open_access
checksum: 40a8fbc3663bf07b37cb80020974d40d
content_type: application/pdf
creator: dernst
date_created: 2023-01-30T11:07:27Z
date_updated: 2023-01-30T11:07:27Z
file_id: '12458'
file_name: 2022_PhysicalReviewX_Brueckner.pdf
file_size: 4686804
relation: main_file
success: 1
file_date_updated: 2023-01-30T11:07:27Z
has_accepted_license: '1'
intvolume: ' 12'
isi: 1
issue: '3'
keyword:
- General Physics and Astronomy
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
publication: Physical Review X
publication_identifier:
issn:
- 2160-3308
publication_status: published
publisher: American Physical Society
quality_controlled: '1'
scopus_import: '1'
status: public
title: Geometry adaptation of protrusion and polarity dynamics in confined cell migration
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 12
year: '2022'
...
---
_id: '12274'
abstract:
- lang: eng
text: The morphology and functionality of the epithelial lining differ along the
intestinal tract, but tissue renewal at all sites is driven by stem cells at the
base of crypts1,2,3. Whether stem cell numbers and behaviour vary at different
sites is unknown. Here we show using intravital microscopy that, despite similarities
in the number and distribution of proliferative cells with an Lgr5 signature in
mice, small intestinal crypts contain twice as many effective stem cells as large
intestinal crypts. We find that, although passively displaced by a conveyor-belt-like
upward movement, small intestinal cells positioned away from the crypt base can
function as long-term effective stem cells owing to Wnt-dependent retrograde cellular
movement. By contrast, the near absence of retrograde movement in the large intestine
restricts cell repositioning, leading to a reduction in effective stem cell number.
Moreover, after suppression of the retrograde movement in the small intestine,
the number of effective stem cells is reduced, and the rate of monoclonal conversion
of crypts is accelerated. Together, these results show that the number of effective
stem cells is determined by active retrograde movement, revealing a new channel
of stem cell regulation that can be experimentally and pharmacologically manipulated.
acknowledgement: We thank the members of the van Rheenen laboratory for reading the
manuscript, and the members of the bioimaging, FACS and animal facility of the NKI
for experimental support. We acknowledge the staff at the MedH Flow Cytometry core
facility, Karolinska Institutet, and LCI facility/Nikon Center of Excellence, Karolinska
Institutet. This work was financially supported by the Netherlands Organization
of Scientific Research NWO (Veni grant 863.15.011 to S.I.J.E. and Vici grant 09150182110004
to J.v.R.) and the CancerGenomics.nl (Netherlands Organisation for Scientific Research)
program (to J.v.R.) the Doctor Josef Steiner Foundation (to J.v.R). B.D.S. acknowledges
funding from the Royal Society E.P. Abraham Research Professorship (RP\R1\180165)
and the Wellcome Trust (098357/Z/12/Z and 219478/Z/19/Z). B.C.-M. acknowledges the
support of the field of excellence ‘Complexity of life in basic research and innovation’
of the University of Graz. O.J.S. and their laboratory acknowledge CRUK core funding
to the CRUK Beatson Institute (A17196 and A31287) and CRUK core funding to the Sansom
laboratory (A21139). P.K. and their laboratory are supported by grants from the
Swedish Research Council (2018-03078), Cancerfonden (190634), Academy of Finland
Centre of Excellence (266869, 304591 and 320185) and the Jane and Aatos Erkko Foundation.
P.L. has received funding from the European Research Council (ERC) under the European
Union’s Horizon 2020 research and innovation programme (grant agreement no. 758617).
E.H. acknowledges funding from the European Research Council (ERC) under the European
Union’s Horizon 2020 research and innovation programme (grant agreement no. 851288).
article_processing_charge: No
article_type: original
author:
- first_name: Maria
full_name: Azkanaz, Maria
last_name: Azkanaz
- first_name: Bernat
full_name: Corominas-Murtra, Bernat
id: 43BE2298-F248-11E8-B48F-1D18A9856A87
last_name: Corominas-Murtra
orcid: 0000-0001-9806-5643
- first_name: Saskia I. J.
full_name: Ellenbroek, Saskia I. J.
last_name: Ellenbroek
- first_name: Lotte
full_name: Bruens, Lotte
last_name: Bruens
- first_name: Anna T.
full_name: Webb, Anna T.
last_name: Webb
- first_name: Dimitrios
full_name: Laskaris, Dimitrios
last_name: Laskaris
- first_name: Koen C.
full_name: Oost, Koen C.
last_name: Oost
- first_name: Simona J. A.
full_name: Lafirenze, Simona J. A.
last_name: Lafirenze
- first_name: Karl
full_name: Annusver, Karl
last_name: Annusver
- first_name: Hendrik A.
full_name: Messal, Hendrik A.
last_name: Messal
- first_name: Sharif
full_name: Iqbal, Sharif
last_name: Iqbal
- first_name: Dustin J.
full_name: Flanagan, Dustin J.
last_name: Flanagan
- first_name: David J.
full_name: Huels, David J.
last_name: Huels
- first_name: Felipe
full_name: Rojas-Rodríguez, Felipe
last_name: Rojas-Rodríguez
- first_name: Miguel
full_name: Vizoso, Miguel
last_name: Vizoso
- first_name: Maria
full_name: Kasper, Maria
last_name: Kasper
- first_name: Owen J.
full_name: Sansom, Owen J.
last_name: Sansom
- first_name: Hugo J.
full_name: Snippert, Hugo J.
last_name: Snippert
- first_name: Prisca
full_name: Liberali, Prisca
last_name: Liberali
- first_name: Benjamin D.
full_name: Simons, Benjamin D.
last_name: Simons
- first_name: Pekka
full_name: Katajisto, Pekka
last_name: Katajisto
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Jacco
full_name: van Rheenen, Jacco
last_name: van Rheenen
citation:
ama: Azkanaz M, Corominas-Murtra B, Ellenbroek SIJ, et al. Retrograde movements
determine effective stem cell numbers in the intestine. Nature. 2022;607(7919):548-554.
doi:10.1038/s41586-022-04962-0
apa: Azkanaz, M., Corominas-Murtra, B., Ellenbroek, S. I. J., Bruens, L., Webb,
A. T., Laskaris, D., … van Rheenen, J. (2022). Retrograde movements determine
effective stem cell numbers in the intestine. Nature. Springer Nature.
https://doi.org/10.1038/s41586-022-04962-0
chicago: Azkanaz, Maria, Bernat Corominas-Murtra, Saskia I. J. Ellenbroek, Lotte
Bruens, Anna T. Webb, Dimitrios Laskaris, Koen C. Oost, et al. “Retrograde Movements
Determine Effective Stem Cell Numbers in the Intestine.” Nature. Springer
Nature, 2022. https://doi.org/10.1038/s41586-022-04962-0.
ieee: M. Azkanaz et al., “Retrograde movements determine effective stem cell
numbers in the intestine,” Nature, vol. 607, no. 7919. Springer Nature,
pp. 548–554, 2022.
ista: Azkanaz M, Corominas-Murtra B, Ellenbroek SIJ, Bruens L, Webb AT, Laskaris
D, Oost KC, Lafirenze SJA, Annusver K, Messal HA, Iqbal S, Flanagan DJ, Huels
DJ, Rojas-Rodríguez F, Vizoso M, Kasper M, Sansom OJ, Snippert HJ, Liberali P,
Simons BD, Katajisto P, Hannezo EB, van Rheenen J. 2022. Retrograde movements
determine effective stem cell numbers in the intestine. Nature. 607(7919), 548–554.
mla: Azkanaz, Maria, et al. “Retrograde Movements Determine Effective Stem Cell
Numbers in the Intestine.” Nature, vol. 607, no. 7919, Springer Nature,
2022, pp. 548–54, doi:10.1038/s41586-022-04962-0.
short: M. Azkanaz, B. Corominas-Murtra, S.I.J. Ellenbroek, L. Bruens, A.T. Webb,
D. Laskaris, K.C. Oost, S.J.A. Lafirenze, K. Annusver, H.A. Messal, S. Iqbal,
D.J. Flanagan, D.J. Huels, F. Rojas-Rodríguez, M. Vizoso, M. Kasper, O.J. Sansom,
H.J. Snippert, P. Liberali, B.D. Simons, P. Katajisto, E.B. Hannezo, J. van Rheenen,
Nature 607 (2022) 548–554.
date_created: 2023-01-16T10:01:29Z
date_published: 2022-07-13T00:00:00Z
date_updated: 2023-10-03T11:16:30Z
day: '13'
department:
- _id: EdHa
doi: 10.1038/s41586-022-04962-0
ec_funded: 1
external_id:
isi:
- '000824430000004'
pmid:
- '35831497'
intvolume: ' 607'
isi: 1
issue: '7919'
keyword:
- Multidisciplinary
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://helda.helsinki.fi/items/94433455-4854-45c0-9de8-7326caea8780
month: '07'
oa: 1
oa_version: Submitted Version
page: 548-554
pmid: 1
project:
- _id: 05943252-7A3F-11EA-A408-12923DDC885E
call_identifier: H2020
grant_number: '851288'
name: Design Principles of Branching Morphogenesis
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- relation: software
url: https://github.com/JaccovanRheenenLab/Retrograde_movement_Azkanaz_Nature_2022
scopus_import: '1'
status: public
title: Retrograde movements determine effective stem cell numbers in the intestine
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 607
year: '2022'
...
---
_id: '13068'
abstract:
- lang: eng
text: Source data and source code for the graphs in "Spatiotemporal dynamics of
self-organized branching pancreatic cancer-derived organoids".
article_processing_charge: No
author:
- first_name: Samuel
full_name: Randriamanantsoa, Samuel
last_name: Randriamanantsoa
- first_name: Aristeidis
full_name: Papargyriou, Aristeidis
last_name: Papargyriou
- first_name: Carlo
full_name: Maurer, Carlo
last_name: Maurer
- first_name: Katja
full_name: Peschke, Katja
last_name: Peschke
- first_name: Maximilian
full_name: Schuster, Maximilian
last_name: Schuster
- first_name: Giulia
full_name: Zecchin, Giulia
last_name: Zecchin
- first_name: Katja
full_name: Steiger, Katja
last_name: Steiger
- first_name: Rupert
full_name: Öllinger, Rupert
last_name: Öllinger
- first_name: Dieter
full_name: Saur, Dieter
last_name: Saur
- first_name: Christina
full_name: Scheel, Christina
last_name: Scheel
- first_name: Roland
full_name: Rad, Roland
last_name: Rad
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Maximilian
full_name: Reichert, Maximilian
last_name: Reichert
- first_name: Andreas R.
full_name: Bausch, Andreas R.
last_name: Bausch
citation:
ama: Randriamanantsoa S, Papargyriou A, Maurer C, et al. Spatiotemporal dynamics
of self-organized branching in pancreas-derived organoids. 2021. doi:10.5281/ZENODO.5148117
apa: Randriamanantsoa, S., Papargyriou, A., Maurer, C., Peschke, K., Schuster, M.,
Zecchin, G., … Bausch, A. R. (2021). Spatiotemporal dynamics of self-organized
branching in pancreas-derived organoids. Zenodo. https://doi.org/10.5281/ZENODO.5148117
chicago: Randriamanantsoa, Samuel, Aristeidis Papargyriou, Carlo Maurer, Katja Peschke,
Maximilian Schuster, Giulia Zecchin, Katja Steiger, et al. “Spatiotemporal Dynamics
of Self-Organized Branching in Pancreas-Derived Organoids.” Zenodo, 2021. https://doi.org/10.5281/ZENODO.5148117.
ieee: S. Randriamanantsoa et al., “Spatiotemporal dynamics of self-organized
branching in pancreas-derived organoids.” Zenodo, 2021.
ista: Randriamanantsoa S, Papargyriou A, Maurer C, Peschke K, Schuster M, Zecchin
G, Steiger K, Öllinger R, Saur D, Scheel C, Rad R, Hannezo EB, Reichert M, Bausch
AR. 2021. Spatiotemporal dynamics of self-organized branching in pancreas-derived
organoids, Zenodo, 10.5281/ZENODO.5148117.
mla: Randriamanantsoa, Samuel, et al. Spatiotemporal Dynamics of Self-Organized
Branching in Pancreas-Derived Organoids. Zenodo, 2021, doi:10.5281/ZENODO.5148117.
short: S. Randriamanantsoa, A. Papargyriou, C. Maurer, K. Peschke, M. Schuster,
G. Zecchin, K. Steiger, R. Öllinger, D. Saur, C. Scheel, R. Rad, E.B. Hannezo,
M. Reichert, A.R. Bausch, (2021).
date_created: 2023-05-23T16:39:24Z
date_published: 2021-07-30T00:00:00Z
date_updated: 2023-08-04T09:25:23Z
day: '30'
ddc:
- '570'
department:
- _id: EdHa
doi: 10.5281/ZENODO.5148117
main_file_link:
- open_access: '1'
url: https://doi.org/10.5281/zenodo.6577226
month: '07'
oa: 1
oa_version: Published Version
publisher: Zenodo
related_material:
record:
- id: '12217'
relation: used_in_publication
status: public
status: public
title: Spatiotemporal dynamics of self-organized branching in pancreas-derived organoids
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: research_data_reference
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2021'
...
---
_id: '8602'
abstract:
- lang: eng
text: Collective cell migration offers a rich field of study for non-equilibrium
physics and cellular biology, revealing phenomena such as glassy dynamics, pattern
formation and active turbulence. However, how mechanical and chemical signalling
are integrated at the cellular level to give rise to such collective behaviours
remains unclear. We address this by focusing on the highly conserved phenomenon
of spatiotemporal waves of density and extracellular signal-regulated kinase (ERK)
activation, which appear both in vitro and in vivo during collective cell migration
and wound healing. First, we propose a biophysical theory, backed by mechanical
and optogenetic perturbation experiments, showing that patterns can be quantitatively
explained by a mechanochemical coupling between active cellular tensions and the
mechanosensitive ERK pathway. Next, we demonstrate how this biophysical mechanism
can robustly induce long-ranged order and migration in a desired orientation,
and we determine the theoretically optimal wavelength and period for inducing
maximal migration towards free edges, which fits well with experimentally observed
dynamics. We thereby provide a bridge between the biophysical origin of spatiotemporal
instabilities and the design principles of robust and efficient long-ranged migration.
acknowledgement: We would like to thank G. Tkacik and all of the members of the Hannezo
and Hirashima groups for useful discussions, X. Trepat for help on traction force
microscopy and M. Matsuda for use of the lab facility. E.H. acknowledges grants
from the Austrian Science Fund (FWF) (P 31639) and the European Research Council
(851288). T.H. acknowledges a grant from JST, PRESTO (JPMJPR1949). This project
has received funding from the European Union’s Horizon 2020 research and innovation
programme under the Marie Skłodowska-Curie grant agreement no. 665385 (to D.B.),
from JSPS KAKENHI grant no. 17J02107 (to N.H.) and from the SPIRITS 2018 of Kyoto
University (to E.H. and T.H.).
article_processing_charge: No
article_type: original
author:
- first_name: Daniel R
full_name: Boocock, Daniel R
id: 453AF628-F248-11E8-B48F-1D18A9856A87
last_name: Boocock
orcid: 0000-0002-1585-2631
- first_name: Naoya
full_name: Hino, Naoya
last_name: Hino
- first_name: Natalia
full_name: Ruzickova, Natalia
id: D2761128-D73D-11E9-A1BF-BA0DE6697425
last_name: Ruzickova
- first_name: Tsuyoshi
full_name: Hirashima, Tsuyoshi
last_name: Hirashima
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
citation:
ama: Boocock DR, Hino N, Ruzickova N, Hirashima T, Hannezo EB. Theory of mechanochemical
patterning and optimal migration in cell monolayers. Nature Physics. 2021;17:267-274.
doi:10.1038/s41567-020-01037-7
apa: Boocock, D. R., Hino, N., Ruzickova, N., Hirashima, T., & Hannezo, E. B.
(2021). Theory of mechanochemical patterning and optimal migration in cell monolayers.
Nature Physics. Springer Nature. https://doi.org/10.1038/s41567-020-01037-7
chicago: Boocock, Daniel R, Naoya Hino, Natalia Ruzickova, Tsuyoshi Hirashima, and
Edouard B Hannezo. “Theory of Mechanochemical Patterning and Optimal Migration
in Cell Monolayers.” Nature Physics. Springer Nature, 2021. https://doi.org/10.1038/s41567-020-01037-7.
ieee: D. R. Boocock, N. Hino, N. Ruzickova, T. Hirashima, and E. B. Hannezo, “Theory
of mechanochemical patterning and optimal migration in cell monolayers,” Nature
Physics, vol. 17. Springer Nature, pp. 267–274, 2021.
ista: Boocock DR, Hino N, Ruzickova N, Hirashima T, Hannezo EB. 2021. Theory of
mechanochemical patterning and optimal migration in cell monolayers. Nature Physics.
17, 267–274.
mla: Boocock, Daniel R., et al. “Theory of Mechanochemical Patterning and Optimal
Migration in Cell Monolayers.” Nature Physics, vol. 17, Springer Nature,
2021, pp. 267–74, doi:10.1038/s41567-020-01037-7.
short: D.R. Boocock, N. Hino, N. Ruzickova, T. Hirashima, E.B. Hannezo, Nature Physics
17 (2021) 267–274.
date_created: 2020-10-04T22:01:37Z
date_published: 2021-02-01T00:00:00Z
date_updated: 2023-08-04T11:02:41Z
day: '01'
department:
- _id: EdHa
doi: 10.1038/s41567-020-01037-7
ec_funded: 1
external_id:
isi:
- '000573519500002'
intvolume: ' 17'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2020.05.15.096479
month: '02'
oa: 1
oa_version: Preprint
page: 267-274
project:
- _id: 268294B6-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P31639
name: Active mechano-chemical description of the cell cytoskeleton
- _id: 05943252-7A3F-11EA-A408-12923DDC885E
call_identifier: H2020
grant_number: '851288'
name: Design Principles of Branching Morphogenesis
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
publication: Nature Physics
publication_identifier:
eissn:
- '17452481'
issn:
- '17452473'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/wound-healing-waves/
record:
- id: '12964'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Theory of mechanochemical patterning and optimal migration in cell monolayers
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 17
year: '2021'
...
---
_id: '9244'
abstract:
- lang: eng
text: 'Organ function depends on tissues adopting the correct architecture. However,
insights into organ architecture are currently hampered by an absence of standardized
quantitative 3D analysis. We aimed to develop a robust technology to visualize,
digitalize, and segment the architecture of two tubular systems in 3D: double
resin casting micro computed tomography (DUCT). As proof of principle, we applied
DUCT to a mouse model for Alagille syndrome (Jag1Ndr/Ndr mice), characterized
by intrahepatic bile duct paucity, that can spontaneously generate a biliary system
in adulthood. DUCT identified increased central biliary branching and peripheral
bile duct tortuosity as two compensatory processes occurring in distinct regions
of Jag1Ndr/Ndr liver, leading to full reconstitution of wild-type biliary volume
and phenotypic recovery. DUCT is thus a powerful new technology for 3D analysis,
which can reveal novel phenotypes and provide a standardized method of defining
liver architecture in mouse models.'
acknowledgement: "Work in ERA lab is supported by the Swedish Research Council, the
Center of Innovative Medicine (CIMED) Grant, Karolinska Institutet, and the Heart
and Lung Foundation, and\r\nthe Daniel Alagille Award from the European Association
for the Study of the Liver. One project in ERA lab is funded by ModeRNA, unrelated
to this project. The funders have no role in the design or interpretation of the
work. SH has been supported by a KI-MU PhD student program, and by a Wera Ekstro¨m
Foundation Scholarship. We are grateful for support from Tornspiran foundation to
NVH. JK: This research was carried out under the project CEITEC 2020 (LQ1601) with
financial support from the Ministry of Education, Youth and Sports of the Czech
Republic under the National Sustainability Programme II and CzechNanoLab Research
Infrastructure supported by MEYS CR (LM2018110) . UL: The financial support from
the Swedish Research Council and ICMC (Integrated CardioMetabolic Center) is acknowledged.
JJ: The work was supported by the Grant Agency of Masaryk University (project no.
MUNI/A/1565/2018). We thank Kari Huppert and Stacey Huppert for their expertise
and help regarding bile duct cannulation and their laboratory hospitality. We also
thank Nadja Schultz and Charlotte L Mattsson for their help with common bile duct
cannulation. We thank Daniel Holl for his help with trachea cannulation. We thank
Nikos Papadogiannakis for his assistance with mild Alagille biopsy samples and discussion.
We thank Karolinska Biomedicum Imaging Core, especially Shigeaki Kanatani for his
help with image analysis. We thank Jan Masek and Carolina Gutierrez for their scientific
input in manuscript writing. We thank Peter Ranefall and the BioImage Informatics
(SciLife national facility) for their help writing parts of the MATLAB pipeline.\r\nThe
TROMA-III antibody developed by Rolf Kemler was obtained from the Developmental
Studies Hybridoma (DSHB) Bank developed under the auspices of NICHD and maintained
by The University of Iowa, Department of Biological Sciences, Iowa City, IA52242.
We thank Goncalo M Brito for all illustrations. This work was supported by the European
Union (European Research Council Starting grant 851288 to E.H.)."
article_number: e60916
article_processing_charge: No
article_type: original
author:
- first_name: Simona
full_name: Hankeova, Simona
last_name: Hankeova
- first_name: Jakub
full_name: Salplachta, Jakub
last_name: Salplachta
- first_name: Tomas
full_name: Zikmund, Tomas
last_name: Zikmund
- first_name: Michaela
full_name: Kavkova, Michaela
last_name: Kavkova
- first_name: Noémi
full_name: Van Hul, Noémi
last_name: Van Hul
- first_name: Adam
full_name: Brinek, Adam
last_name: Brinek
- first_name: Veronika
full_name: Smekalova, Veronika
last_name: Smekalova
- first_name: Jakub
full_name: Laznovsky, Jakub
last_name: Laznovsky
- first_name: Feven
full_name: Dawit, Feven
last_name: Dawit
- first_name: Josef
full_name: Jaros, Josef
last_name: Jaros
- first_name: Vítězslav
full_name: Bryja, Vítězslav
last_name: Bryja
- first_name: Urban
full_name: Lendahl, Urban
last_name: Lendahl
- first_name: Ewa
full_name: Ellis, Ewa
last_name: Ellis
- first_name: Antal
full_name: Nemeth, Antal
last_name: Nemeth
- first_name: Björn
full_name: Fischler, Björn
last_name: Fischler
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Jozef
full_name: Kaiser, Jozef
last_name: Kaiser
- first_name: Emma Rachel
full_name: Andersson, Emma Rachel
last_name: Andersson
citation:
ama: Hankeova S, Salplachta J, Zikmund T, et al. DUCT reveals architectural mechanisms
contributing to bile duct recovery in a mouse model for alagille syndrome. eLife.
2021;10. doi:10.7554/eLife.60916
apa: Hankeova, S., Salplachta, J., Zikmund, T., Kavkova, M., Van Hul, N., Brinek,
A., … Andersson, E. R. (2021). DUCT reveals architectural mechanisms contributing
to bile duct recovery in a mouse model for alagille syndrome. ELife. eLife
Sciences Publications. https://doi.org/10.7554/eLife.60916
chicago: Hankeova, Simona, Jakub Salplachta, Tomas Zikmund, Michaela Kavkova, Noémi
Van Hul, Adam Brinek, Veronika Smekalova, et al. “DUCT Reveals Architectural Mechanisms
Contributing to Bile Duct Recovery in a Mouse Model for Alagille Syndrome.” ELife.
eLife Sciences Publications, 2021. https://doi.org/10.7554/eLife.60916.
ieee: S. Hankeova et al., “DUCT reveals architectural mechanisms contributing
to bile duct recovery in a mouse model for alagille syndrome,” eLife, vol.
10. eLife Sciences Publications, 2021.
ista: Hankeova S, Salplachta J, Zikmund T, Kavkova M, Van Hul N, Brinek A, Smekalova
V, Laznovsky J, Dawit F, Jaros J, Bryja V, Lendahl U, Ellis E, Nemeth A, Fischler
B, Hannezo EB, Kaiser J, Andersson ER. 2021. DUCT reveals architectural mechanisms
contributing to bile duct recovery in a mouse model for alagille syndrome. eLife.
10, e60916.
mla: Hankeova, Simona, et al. “DUCT Reveals Architectural Mechanisms Contributing
to Bile Duct Recovery in a Mouse Model for Alagille Syndrome.” ELife, vol.
10, e60916, eLife Sciences Publications, 2021, doi:10.7554/eLife.60916.
short: S. Hankeova, J. Salplachta, T. Zikmund, M. Kavkova, N. Van Hul, A. Brinek,
V. Smekalova, J. Laznovsky, F. Dawit, J. Jaros, V. Bryja, U. Lendahl, E. Ellis,
A. Nemeth, B. Fischler, E.B. Hannezo, J. Kaiser, E.R. Andersson, ELife 10 (2021).
date_created: 2021-03-14T23:01:34Z
date_published: 2021-02-26T00:00:00Z
date_updated: 2023-08-07T14:12:54Z
day: '26'
ddc:
- '570'
department:
- _id: EdHa
doi: 10.7554/eLife.60916
ec_funded: 1
external_id:
isi:
- '000625357100001'
pmid:
- '33635272'
file:
- access_level: open_access
checksum: 20ccf4dfe46c48cf986794c8bf4fd1cb
content_type: application/pdf
creator: dernst
date_created: 2021-03-22T08:50:33Z
date_updated: 2021-03-22T08:50:33Z
file_id: '9271'
file_name: 2021_eLife_Hankeova.pdf
file_size: 9259690
relation: main_file
success: 1
file_date_updated: 2021-03-22T08:50:33Z
has_accepted_license: '1'
intvolume: ' 10'
isi: 1
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 05943252-7A3F-11EA-A408-12923DDC885E
call_identifier: H2020
grant_number: '851288'
name: Design Principles of Branching Morphogenesis
publication: eLife
publication_identifier:
eissn:
- 2050084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: DUCT reveals architectural mechanisms contributing to bile duct recovery in
a mouse model for alagille syndrome
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 10
year: '2021'
...