[{"date_updated":"2021-01-12T08:03:30Z","date_created":"2019-01-06T22:59:11Z","volume":1893,"oa_version":"None","author":[{"first_name":"Yoichi","last_name":"Asaoka","full_name":"Asaoka, Yoichi"},{"full_name":"Morita, Hitoshi","first_name":"Hitoshi","last_name":"Morita"},{"full_name":"Furumoto, Hiroko","last_name":"Furumoto","first_name":"Hiroko"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Makoto","last_name":"Furutani-Seiki","full_name":"Furutani-Seiki, Makoto"}],"status":"public","publication_status":"published","title":"Studying YAP-mediated 3D morphogenesis using fish embryos and human spheroids","intvolume":" 1893","department":[{"_id":"CaHe"}],"editor":[{"full_name":"Hergovich, Alexander","last_name":"Hergovich","first_name":"Alexander"}],"publisher":"Springer","year":"2019","_id":"5793","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","abstract":[{"text":"The transcription coactivator, Yes-associated protein (YAP), which is a nuclear effector of the Hippo signaling pathway, has been shown to be a mechano-transducer. By using mutant fish and human 3D spheroids, we have recently demonstrated that YAP is also a mechano-effector. YAP functions in three-dimensional (3D) morphogenesis of organ and global body shape by controlling actomyosin-mediated tissue tension. In this chapter, we present a platform that links the findings in fish embryos with human cells. The protocols for analyzing tissue tension-mediated global body shape/organ morphogenesis in vivo and ex vivo using medaka fish embryos and in vitro using human cell spheroids represent useful tools for unraveling the molecular mechanisms by which YAP functions in regulating global body/organ morphogenesis.","lang":"eng"}],"alternative_title":["MIMB"],"type":"book_chapter","language":[{"iso":"eng"}],"doi":"10.1007/978-1-4939-8910-2_14","date_published":"2019-01-01T00:00:00Z","quality_controlled":"1","page":"167-181","publication":"The hippo pathway","citation":{"ama":"Asaoka Y, Morita H, Furumoto H, Heisenberg C-PJ, Furutani-Seiki M. Studying YAP-mediated 3D morphogenesis using fish embryos and human spheroids. In: Hergovich A, ed. The Hippo Pathway. Vol 1893. Methods in Molecular Biology. Springer; 2019:167-181. doi:10.1007/978-1-4939-8910-2_14","apa":"Asaoka, Y., Morita, H., Furumoto, H., Heisenberg, C.-P. J., & Furutani-Seiki, M. (2019). Studying YAP-mediated 3D morphogenesis using fish embryos and human spheroids. In A. Hergovich (Ed.), The hippo pathway (Vol. 1893, pp. 167–181). Springer. https://doi.org/10.1007/978-1-4939-8910-2_14","ieee":"Y. Asaoka, H. Morita, H. Furumoto, C.-P. J. Heisenberg, and M. Furutani-Seiki, “Studying YAP-mediated 3D morphogenesis using fish embryos and human spheroids,” in The hippo pathway, vol. 1893, A. Hergovich, Ed. Springer, 2019, pp. 167–181.","ista":"Asaoka Y, Morita H, Furumoto H, Heisenberg C-PJ, Furutani-Seiki M. 2019.Studying YAP-mediated 3D morphogenesis using fish embryos and human spheroids. In: The hippo pathway. MIMB, vol. 1893, 167–181.","short":"Y. Asaoka, H. Morita, H. Furumoto, C.-P.J. Heisenberg, M. Furutani-Seiki, in:, A. Hergovich (Ed.), The Hippo Pathway, Springer, 2019, pp. 167–181.","mla":"Asaoka, Yoichi, et al. “Studying YAP-Mediated 3D Morphogenesis Using Fish Embryos and Human Spheroids.” The Hippo Pathway, edited by Alexander Hergovich, vol. 1893, Springer, 2019, pp. 167–81, doi:10.1007/978-1-4939-8910-2_14.","chicago":"Asaoka, Yoichi, Hitoshi Morita, Hiroko Furumoto, Carl-Philipp J Heisenberg, and Makoto Furutani-Seiki. “Studying YAP-Mediated 3D Morphogenesis Using Fish Embryos and Human Spheroids.” In The Hippo Pathway, edited by Alexander Hergovich, 1893:167–81. Methods in Molecular Biology. Springer, 2019. https://doi.org/10.1007/978-1-4939-8910-2_14."},"day":"01","month":"01","publication_identifier":{"isbn":["978-1-4939-8909-6"]},"series_title":"Methods in Molecular Biology","scopus_import":1},{"abstract":[{"lang":"eng","text":"Non-canonical Wnt signaling plays a central role for coordinated cell polarization and directed migration in metazoan development. While spatiotemporally restricted activation of non-canonical Wnt-signaling drives cell polarization in epithelial tissues, it remains unclear whether such instructive activity is also critical for directed mesenchymal cell migration. Here, we developed a light-activated version of the non-canonical Wnt receptor Frizzled 7 (Fz7) to analyze how restricted activation of non-canonical Wnt signaling affects directed anterior axial mesendoderm (prechordal plate, ppl) cell migration within the zebrafish gastrula. We found that Fz7 signaling is required for ppl cell protrusion formation and migration and that spatiotemporally restricted ectopic activation is capable of redirecting their migration. Finally, we show that uniform activation of Fz7 signaling in ppl cells fully rescues defective directed cell migration in fz7 mutant embryos. Together, our findings reveal that in contrast to the situation in epithelial cells, non-canonical Wnt signaling functions permissively rather than instructively in directed mesenchymal cell migration during gastrulation."}],"type":"journal_article","oa_version":"Published Version","file":[{"content_type":"application/pdf","file_size":5500707,"creator":"dernst","file_name":"2019_elife_Capek.pdf","access_level":"open_access","date_created":"2019-02-18T15:17:21Z","date_updated":"2020-07-14T12:47:17Z","checksum":"6cb4ca6d4aa96f6f187a5983aa3e660a","relation":"main_file","file_id":"6041"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"6025","intvolume":" 8","status":"public","ddc":["570"],"title":"Light-activated Frizzled7 reveals a permissive role of non-canonical wnt signaling in mesendoderm cell migration","has_accepted_license":"1","article_processing_charge":"No","day":"06","scopus_import":"1","date_published":"2019-02-06T00:00:00Z","citation":{"ista":"Capek D, Smutny M, Tichy AM, Morri M, Janovjak HL, Heisenberg C-PJ. 2019. Light-activated Frizzled7 reveals a permissive role of non-canonical wnt signaling in mesendoderm cell migration. eLife. 8, e42093.","apa":"Capek, D., Smutny, M., Tichy, A. M., Morri, M., Janovjak, H. L., & Heisenberg, C.-P. J. (2019). Light-activated Frizzled7 reveals a permissive role of non-canonical wnt signaling in mesendoderm cell migration. ELife. eLife Sciences Publications. https://doi.org/10.7554/eLife.42093","ieee":"D. Capek, M. Smutny, A. M. Tichy, M. Morri, H. L. Janovjak, and C.-P. J. Heisenberg, “Light-activated Frizzled7 reveals a permissive role of non-canonical wnt signaling in mesendoderm cell migration,” eLife, vol. 8. eLife Sciences Publications, 2019.","ama":"Capek D, Smutny M, Tichy AM, Morri M, Janovjak HL, Heisenberg C-PJ. Light-activated Frizzled7 reveals a permissive role of non-canonical wnt signaling in mesendoderm cell migration. eLife. 2019;8. doi:10.7554/eLife.42093","chicago":"Capek, Daniel, Michael Smutny, Alexandra Madelaine Tichy, Maurizio Morri, Harald L Janovjak, and Carl-Philipp J Heisenberg. “Light-Activated Frizzled7 Reveals a Permissive Role of Non-Canonical Wnt Signaling in Mesendoderm Cell Migration.” ELife. eLife Sciences Publications, 2019. https://doi.org/10.7554/eLife.42093.","mla":"Capek, Daniel, et al. “Light-Activated Frizzled7 Reveals a Permissive Role of Non-Canonical Wnt Signaling in Mesendoderm Cell Migration.” ELife, vol. 8, e42093, eLife Sciences Publications, 2019, doi:10.7554/eLife.42093.","short":"D. Capek, M. Smutny, A.M. Tichy, M. Morri, H.L. Janovjak, C.-P.J. Heisenberg, ELife 8 (2019)."},"publication":"eLife","ec_funded":1,"file_date_updated":"2020-07-14T12:47:17Z","article_number":"e42093","author":[{"full_name":"Capek, Daniel","orcid":"0000-0001-5199-9940","id":"31C42484-F248-11E8-B48F-1D18A9856A87","last_name":"Capek","first_name":"Daniel"},{"id":"3FE6E4E8-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5920-9090","first_name":"Michael","last_name":"Smutny","full_name":"Smutny, Michael"},{"last_name":"Tichy","first_name":"Alexandra Madelaine","full_name":"Tichy, Alexandra Madelaine"},{"id":"4863116E-F248-11E8-B48F-1D18A9856A87","first_name":"Maurizio","last_name":"Morri","full_name":"Morri, Maurizio"},{"id":"33BA6C30-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8023-9315","first_name":"Harald L","last_name":"Janovjak","full_name":"Janovjak, Harald L"},{"full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J"}],"volume":8,"date_created":"2019-02-17T22:59:22Z","date_updated":"2023-08-24T14:46:01Z","year":"2019","publisher":"eLife Sciences Publications","department":[{"_id":"CaHe"},{"_id":"HaJa"}],"publication_status":"published","month":"02","doi":"10.7554/eLife.42093","language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"external_id":{"isi":["000458025300001"]},"project":[{"name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","call_identifier":"H2020","_id":"260F1432-B435-11E9-9278-68D0E5697425","grant_number":"742573"}],"quality_controlled":"1","isi":1},{"month":"03","language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"LifeSc"}],"doi":"10.1016/j.cell.2019.01.019","project":[{"_id":"260F1432-B435-11E9-9278-68D0E5697425","grant_number":"742573","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","call_identifier":"H2020"}],"isi":1,"quality_controlled":"1","external_id":{"pmid":["30773315"],"isi":["000460509600013"]},"main_file_link":[{"url":"https://doi.org/10.1016/j.cell.2019.01.019","open_access":"1"}],"oa":1,"ec_funded":1,"volume":176,"date_updated":"2023-08-25T08:02:23Z","date_created":"2019-03-10T22:59:19Z","related_material":{"link":[{"description":"News on IST Homepage","relation":"press_release","url":"https://ist.ac.at/en/news/in-zebrafish-eggs-most-rapidly-growing-cell-inhibits-its-neighbours-through-mechanical-signals/"}]},"author":[{"first_name":"Peng","last_name":"Xia","id":"4AB6C7D0-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5419-7756","full_name":"Xia, Peng"},{"first_name":"Daniel J","last_name":"Gütl","id":"381929CE-F248-11E8-B48F-1D18A9856A87","full_name":"Gütl, Daniel J"},{"first_name":"Vanessa","last_name":"Zheden","id":"39C5A68A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-9438-4783","full_name":"Zheden, Vanessa"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"publisher":"Elsevier","department":[{"_id":"CaHe"},{"_id":"EM-Fac"}],"publication_status":"published","pmid":1,"year":"2019","acknowledgement":"We thank Roland Dosch, Makoto Furutani-Seiki, Brian Link, Mary Mullins, and Masazumi Tada for providing transgenic and/or mutant zebrafish lines; Alexandra Schauer, Shayan Shami-Pour, and the rest of the Heisenberg lab for technical assistance and feedback on the manuscript; and the Bioimaging, Electron Microscopy, and Zebrafish facilities of IST Austria for continuous support. This work was supported by an ERC advanced grant ( MECSPEC to C.-P.H.).","article_processing_charge":"No","day":"07","scopus_import":"1","date_published":"2019-03-07T00:00:00Z","page":"1379-1392.e14","article_type":"original","citation":{"short":"P. Xia, D.J. Gütl, V. Zheden, C.-P.J. Heisenberg, Cell 176 (2019) 1379–1392.e14.","mla":"Xia, Peng, et al. “Lateral Inhibition in Cell Specification Mediated by Mechanical Signals Modulating TAZ Activity.” Cell, vol. 176, no. 6, Elsevier, 2019, p. 1379–1392.e14, doi:10.1016/j.cell.2019.01.019.","chicago":"Xia, Peng, Daniel J Gütl, Vanessa Zheden, and Carl-Philipp J Heisenberg. “Lateral Inhibition in Cell Specification Mediated by Mechanical Signals Modulating TAZ Activity.” Cell. Elsevier, 2019. https://doi.org/10.1016/j.cell.2019.01.019.","ama":"Xia P, Gütl DJ, Zheden V, Heisenberg C-PJ. Lateral inhibition in cell specification mediated by mechanical signals modulating TAZ activity. Cell. 2019;176(6):1379-1392.e14. doi:10.1016/j.cell.2019.01.019","apa":"Xia, P., Gütl, D. J., Zheden, V., & Heisenberg, C.-P. J. (2019). Lateral inhibition in cell specification mediated by mechanical signals modulating TAZ activity. Cell. Elsevier. https://doi.org/10.1016/j.cell.2019.01.019","ieee":"P. Xia, D. J. Gütl, V. Zheden, and C.-P. J. Heisenberg, “Lateral inhibition in cell specification mediated by mechanical signals modulating TAZ activity,” Cell, vol. 176, no. 6. Elsevier, p. 1379–1392.e14, 2019.","ista":"Xia P, Gütl DJ, Zheden V, Heisenberg C-PJ. 2019. Lateral inhibition in cell specification mediated by mechanical signals modulating TAZ activity. Cell. 176(6), 1379–1392.e14."},"publication":"Cell","issue":"6","abstract":[{"text":"Cell fate specification by lateral inhibition typically involves contact signaling through the Delta-Notch signaling pathway. However, whether this is the only signaling mode mediating lateral inhibition remains unclear. Here we show that in zebrafish oogenesis, a group of cells within the granulosa cell layer at the oocyte animal pole acquire elevated levels of the transcriptional coactivator TAZ in their nuclei. One of these cells, the future micropyle precursor cell (MPC), accumulates increasingly high levels of nuclear TAZ and grows faster than its surrounding cells, mechanically compressing those cells, which ultimately lose TAZ from their nuclei. Strikingly, relieving neighbor-cell compression by MPC ablation or aspiration restores nuclear TAZ accumulation in neighboring cells, eventually leading to MPC re-specification from these cells. Conversely, MPC specification is defective in taz−/− follicles. These findings uncover a novel mode of lateral inhibition in cell fate specification based on mechanical signals controlling TAZ activity.","lang":"eng"}],"type":"journal_article","oa_version":"Published Version","intvolume":" 176","title":"Lateral inhibition in cell specification mediated by mechanical signals modulating TAZ activity","status":"public","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"6087"},{"ec_funded":1,"date_updated":"2023-08-28T12:25:21Z","date_created":"2019-06-30T21:59:11Z","volume":178,"author":[{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561","first_name":"Edouard B","last_name":"Hannezo","full_name":"Hannezo, Edouard B"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg"}],"publication_status":"published","publisher":"Elsevier","department":[{"_id":"CaHe"},{"_id":"EdHa"}],"year":"2019","pmid":1,"month":"07","publication_identifier":{"issn":["00928674"]},"language":[{"iso":"eng"}],"doi":"10.1016/j.cell.2019.05.052","quality_controlled":"1","isi":1,"project":[{"_id":"260F1432-B435-11E9-9278-68D0E5697425","grant_number":"742573","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","call_identifier":"H2020"},{"_id":"268294B6-B435-11E9-9278-68D0E5697425","grant_number":"P31639","name":"Active mechano-chemical description of the cell cytoskeleton","call_identifier":"FWF"}],"oa":1,"main_file_link":[{"url":"https://doi.org/10.1016/j.cell.2019.05.052","open_access":"1"}],"external_id":{"pmid":["31251912"],"isi":["000473002700005"]},"abstract":[{"text":"There is increasing evidence that both mechanical and biochemical signals play important roles in development and disease. The development of complex organisms, in particular, has been proposed to rely on the feedback between mechanical and biochemical patterning events. This feedback occurs at the molecular level via mechanosensation but can also arise as an emergent property of the system at the cellular and tissue level. In recent years, dynamic changes in tissue geometry, flow, rheology, and cell fate specification have emerged as key platforms of mechanochemical feedback loops in multiple processes. Here, we review recent experimental and theoretical advances in understanding how these feedbacks function in development and disease.","lang":"eng"}],"issue":"1","type":"journal_article","oa_version":"Published Version","title":"Mechanochemical feedback loops in development and disease","status":"public","intvolume":" 178","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"6601","day":"27","article_processing_charge":"No","scopus_import":"1","date_published":"2019-07-27T00:00:00Z","article_type":"review","page":"12-25","publication":"Cell","citation":{"ista":"Hannezo EB, Heisenberg C-PJ. 2019. Mechanochemical feedback loops in development and disease. Cell. 178(1), 12–25.","ieee":"E. B. Hannezo and C.-P. J. Heisenberg, “Mechanochemical feedback loops in development and disease,” Cell, vol. 178, no. 1. Elsevier, pp. 12–25, 2019.","apa":"Hannezo, E. B., & Heisenberg, C.-P. J. (2019). Mechanochemical feedback loops in development and disease. Cell. Elsevier. https://doi.org/10.1016/j.cell.2019.05.052","ama":"Hannezo EB, Heisenberg C-PJ. Mechanochemical feedback loops in development and disease. Cell. 2019;178(1):12-25. doi:10.1016/j.cell.2019.05.052","chicago":"Hannezo, Edouard B, and Carl-Philipp J Heisenberg. “Mechanochemical Feedback Loops in Development and Disease.” Cell. Elsevier, 2019. https://doi.org/10.1016/j.cell.2019.05.052.","mla":"Hannezo, Edouard B., and Carl-Philipp J. Heisenberg. “Mechanochemical Feedback Loops in Development and Disease.” Cell, vol. 178, no. 1, Elsevier, 2019, pp. 12–25, doi:10.1016/j.cell.2019.05.052.","short":"E.B. Hannezo, C.-P.J. Heisenberg, Cell 178 (2019) 12–25."}},{"date_updated":"2023-08-29T06:33:14Z","date_created":"2019-07-14T21:59:17Z","oa_version":"None","volume":60,"author":[{"id":"33280250-F248-11E8-B48F-1D18A9856A87","last_name":"Godard","first_name":"Benoit G","full_name":"Godard, Benoit G"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg"}],"publication_status":"published","status":"public","title":"Cell division and tissue mechanics","publisher":"Elsevier","department":[{"_id":"CaHe"}],"intvolume":" 60","_id":"6631","year":"2019","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","abstract":[{"lang":"eng","text":"The spatiotemporal organization of cell divisions constitutes an integral part in the development of multicellular organisms, and mis-regulation of cell divisions can lead to severe developmental defects. Cell divisions have an important morphogenetic function in development by regulating growth and shape acquisition of developing tissues, and, conversely, tissue morphogenesis is known to affect both the rate and orientation of cell divisions. Moreover, cell divisions are associated with an extensive reorganization of the cytoskeleton and adhesion apparatus in the dividing cells that in turn can affect large-scale tissue rheological properties. Thus, the interplay between cell divisions and tissue morphogenesis plays a key role in embryo and tissue morphogenesis."}],"type":"journal_article","language":[{"iso":"eng"}],"doi":"10.1016/j.ceb.2019.05.007","date_published":"2019-10-01T00:00:00Z","quality_controlled":"1","isi":1,"page":"114-120","publication":"Current Opinion in Cell Biology","citation":{"ista":"Godard BG, Heisenberg C-PJ. 2019. Cell division and tissue mechanics. Current Opinion in Cell Biology. 60, 114–120.","apa":"Godard, B. G., & Heisenberg, C.-P. J. (2019). Cell division and tissue mechanics. Current Opinion in Cell Biology. Elsevier. https://doi.org/10.1016/j.ceb.2019.05.007","ieee":"B. G. Godard and C.-P. J. Heisenberg, “Cell division and tissue mechanics,” Current Opinion in Cell Biology, vol. 60. Elsevier, pp. 114–120, 2019.","ama":"Godard BG, Heisenberg C-PJ. Cell division and tissue mechanics. Current Opinion in Cell Biology. 2019;60:114-120. doi:10.1016/j.ceb.2019.05.007","chicago":"Godard, Benoit G, and Carl-Philipp J Heisenberg. “Cell Division and Tissue Mechanics.” Current Opinion in Cell Biology. Elsevier, 2019. https://doi.org/10.1016/j.ceb.2019.05.007.","mla":"Godard, Benoit G., and Carl-Philipp J. Heisenberg. “Cell Division and Tissue Mechanics.” Current Opinion in Cell Biology, vol. 60, Elsevier, 2019, pp. 114–20, doi:10.1016/j.ceb.2019.05.007.","short":"B.G. Godard, C.-P.J. Heisenberg, Current Opinion in Cell Biology 60 (2019) 114–120."},"external_id":{"isi":["000486545800016"]},"day":"01","month":"10","publication_identifier":{"issn":["0955-0674"]},"article_processing_charge":"No","scopus_import":"1"},{"department":[{"_id":"CaHe"}],"publisher":"Springer Nature","publication_status":"published","pmid":1,"year":"2019","volume":21,"date_created":"2019-09-01T22:00:57Z","date_updated":"2023-08-29T07:42:20Z","author":[{"full_name":"Tavano, Ste","last_name":"Tavano","first_name":"Ste","orcid":"0000-0001-9970-7804","id":"2F162F0C-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J"}],"quality_controlled":"1","isi":1,"external_id":{"pmid":["31371826"],"isi":["000478029000003"]},"language":[{"iso":"eng"}],"doi":"10.1038/s41556-019-0369-3","publication_identifier":{"eissn":["1476-4679"]},"month":"08","intvolume":" 21","status":"public","title":"Migrasomes take center stage","_id":"6837","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","oa_version":"None","type":"journal_article","issue":"8","abstract":[{"text":"Migrasomes are a recently discovered type of extracellular vesicles that are characteristically generated along retraction fibers in migrating cells. Two studies now show how migrasomes are formed and how they function in the physiologically relevant context of the developing zebrafish embryo.","lang":"eng"}],"page":"918-920","citation":{"apa":"Tavano, S., & Heisenberg, C.-P. J. (2019). Migrasomes take center stage. Nature Cell Biology. Springer Nature. https://doi.org/10.1038/s41556-019-0369-3","ieee":"S. Tavano and C.-P. J. Heisenberg, “Migrasomes take center stage,” Nature Cell Biology, vol. 21, no. 8. Springer Nature, pp. 918–920, 2019.","ista":"Tavano S, Heisenberg C-PJ. 2019. Migrasomes take center stage. Nature Cell Biology. 21(8), 918–920.","ama":"Tavano S, Heisenberg C-PJ. Migrasomes take center stage. Nature Cell Biology. 2019;21(8):918-920. doi:10.1038/s41556-019-0369-3","chicago":"Tavano, Ste, and Carl-Philipp J Heisenberg. “Migrasomes Take Center Stage.” Nature Cell Biology. Springer Nature, 2019. https://doi.org/10.1038/s41556-019-0369-3.","short":"S. Tavano, C.-P.J. Heisenberg, Nature Cell Biology 21 (2019) 918–920.","mla":"Tavano, Ste, and Carl-Philipp J. Heisenberg. “Migrasomes Take Center Stage.” Nature Cell Biology, vol. 21, no. 8, Springer Nature, 2019, pp. 918–20, doi:10.1038/s41556-019-0369-3."},"publication":"Nature Cell Biology","date_published":"2019-08-01T00:00:00Z","scopus_import":"1","article_processing_charge":"No","day":"01"},{"ddc":["570"],"title":"Biomechanical signaling within the developing zebrafish heart attunes endocardial growth to myocardial chamber dimensions","status":"public","intvolume":" 10","_id":"6899","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","oa_version":"Published Version","file":[{"file_id":"6926","relation":"main_file","date_updated":"2020-07-14T12:47:44Z","date_created":"2019-10-01T11:18:50Z","checksum":"62c2512712e16d27c1797d318d14ba9f","file_name":"2019_Nature_Bornhorst.pdf","access_level":"open_access","creator":"kschuh","file_size":3905793,"content_type":"application/pdf"}],"type":"journal_article","abstract":[{"lang":"eng","text":"Intra-organ communication guides morphogenetic processes that are essential for an organ to carry out complex physiological functions. In the heart, the growth of the myocardium is tightly coupled to that of the endocardium, a specialized endothelial tissue that lines its interior. Several molecular pathways have been implicated in the communication between these tissues including secreted factors, components of the extracellular matrix, or proteins involved in cell-cell communication. Yet, it is unknown how the growth of the endocardium is coordinated with that of the myocardium. Here, we show that an increased expansion of the myocardial atrial chamber volume generates higher junctional forces within endocardial cells. This leads to biomechanical signaling involving VE-cadherin, triggering nuclear localization of the Hippo pathway transcriptional regulator Yap1 and endocardial proliferation. Our work suggests that the growth of the endocardium results from myocardial chamber volume expansion and ends when the tension on the tissue is relaxed."}],"issue":"1","page":"4113","publication":"Nature communications","citation":{"ama":"Bornhorst D, Xia P, Nakajima H, et al. Biomechanical signaling within the developing zebrafish heart attunes endocardial growth to myocardial chamber dimensions. Nature communications. 2019;10(1):4113. doi:10.1038/s41467-019-12068-x","apa":"Bornhorst, D., Xia, P., Nakajima, H., Dingare, C., Herzog, W., Lecaudey, V., … Abdelilah-Seyfried, S. (2019). Biomechanical signaling within the developing zebrafish heart attunes endocardial growth to myocardial chamber dimensions. Nature Communications. Nature Publishing Group. https://doi.org/10.1038/s41467-019-12068-x","ieee":"D. Bornhorst et al., “Biomechanical signaling within the developing zebrafish heart attunes endocardial growth to myocardial chamber dimensions,” Nature communications, vol. 10, no. 1. Nature Publishing Group, p. 4113, 2019.","ista":"Bornhorst D, Xia P, Nakajima H, Dingare C, Herzog W, Lecaudey V, Mochizuki N, Heisenberg C-PJ, Yelon D, Abdelilah-Seyfried S. 2019. Biomechanical signaling within the developing zebrafish heart attunes endocardial growth to myocardial chamber dimensions. Nature communications. 10(1), 4113.","short":"D. Bornhorst, P. Xia, H. Nakajima, C. Dingare, W. Herzog, V. Lecaudey, N. Mochizuki, C.-P.J. Heisenberg, D. Yelon, S. Abdelilah-Seyfried, Nature Communications 10 (2019) 4113.","mla":"Bornhorst, Dorothee, et al. “Biomechanical Signaling within the Developing Zebrafish Heart Attunes Endocardial Growth to Myocardial Chamber Dimensions.” Nature Communications, vol. 10, no. 1, Nature Publishing Group, 2019, p. 4113, doi:10.1038/s41467-019-12068-x.","chicago":"Bornhorst, Dorothee, Peng Xia, Hiroyuki Nakajima, Chaitanya Dingare, Wiebke Herzog, Virginie Lecaudey, Naoki Mochizuki, Carl-Philipp J Heisenberg, Deborah Yelon, and Salim Abdelilah-Seyfried. “Biomechanical Signaling within the Developing Zebrafish Heart Attunes Endocardial Growth to Myocardial Chamber Dimensions.” Nature Communications. Nature Publishing Group, 2019. https://doi.org/10.1038/s41467-019-12068-x."},"date_published":"2019-09-11T00:00:00Z","scopus_import":"1","day":"11","article_processing_charge":"No","has_accepted_license":"1","publication_status":"published","publisher":"Nature Publishing Group","department":[{"_id":"CaHe"}],"year":"2019","pmid":1,"date_created":"2019-09-22T22:00:37Z","date_updated":"2023-08-30T06:21:23Z","volume":10,"author":[{"full_name":"Bornhorst, Dorothee","first_name":"Dorothee","last_name":"Bornhorst"},{"orcid":"0000-0002-5419-7756","id":"4AB6C7D0-F248-11E8-B48F-1D18A9856A87","last_name":"Xia","first_name":"Peng","full_name":"Xia, Peng"},{"last_name":"Nakajima","first_name":"Hiroyuki","full_name":"Nakajima, Hiroyuki"},{"full_name":"Dingare, Chaitanya","first_name":"Chaitanya","last_name":"Dingare"},{"full_name":"Herzog, Wiebke","first_name":"Wiebke","last_name":"Herzog"},{"last_name":"Lecaudey","first_name":"Virginie","full_name":"Lecaudey, Virginie"},{"last_name":"Mochizuki","first_name":"Naoki","full_name":"Mochizuki, Naoki"},{"full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J"},{"full_name":"Yelon, Deborah","last_name":"Yelon","first_name":"Deborah"},{"full_name":"Abdelilah-Seyfried, Salim","last_name":"Abdelilah-Seyfried","first_name":"Salim"}],"file_date_updated":"2020-07-14T12:47:44Z","quality_controlled":"1","isi":1,"oa":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"external_id":{"pmid":["31511517"],"isi":["000485216800009"]},"language":[{"iso":"eng"}],"doi":"10.1038/s41467-019-12068-x","month":"09","publication_identifier":{"eissn":["20411723"]}},{"article_type":"review","publication":"The EMBO Journal","citation":{"ista":"Petridou N, Heisenberg C-PJ. 2019. Tissue rheology in embryonic organization. The EMBO Journal. 38(20), e102497.","ieee":"N. Petridou and C.-P. J. Heisenberg, “Tissue rheology in embryonic organization,” The EMBO Journal, vol. 38, no. 20. EMBO, 2019.","apa":"Petridou, N., & Heisenberg, C.-P. J. (2019). Tissue rheology in embryonic organization. The EMBO Journal. EMBO. https://doi.org/10.15252/embj.2019102497","ama":"Petridou N, Heisenberg C-PJ. Tissue rheology in embryonic organization. The EMBO Journal. 2019;38(20). doi:10.15252/embj.2019102497","chicago":"Petridou, Nicoletta, and Carl-Philipp J Heisenberg. “Tissue Rheology in Embryonic Organization.” The EMBO Journal. EMBO, 2019. https://doi.org/10.15252/embj.2019102497.","mla":"Petridou, Nicoletta, and Carl-Philipp J. Heisenberg. “Tissue Rheology in Embryonic Organization.” The EMBO Journal, vol. 38, no. 20, e102497, EMBO, 2019, doi:10.15252/embj.2019102497.","short":"N. Petridou, C.-P.J. Heisenberg, The EMBO Journal 38 (2019)."},"date_published":"2019-10-15T00:00:00Z","scopus_import":"1","day":"15","article_processing_charge":"Yes (via OA deal)","has_accepted_license":"1","status":"public","ddc":["570"],"title":"Tissue rheology in embryonic organization","intvolume":" 38","_id":"6980","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","oa_version":"Published Version","file":[{"file_id":"6981","relation":"main_file","checksum":"76f7f4e79ab6d850c30017a69726fd85","date_updated":"2020-07-14T12:47:46Z","date_created":"2019-11-04T15:30:08Z","access_level":"open_access","file_name":"2019_Embo_Petridou.pdf","creator":"dernst","file_size":847356,"content_type":"application/pdf"}],"type":"journal_article","abstract":[{"text":"Tissue morphogenesis in multicellular organisms is brought about by spatiotemporal coordination of mechanical and chemical signals. Extensive work on how mechanical forces together with the well‐established morphogen signalling pathways can actively shape living tissues has revealed evolutionary conserved mechanochemical features of embryonic development. More recently, attention has been drawn to the description of tissue material properties and how they can influence certain morphogenetic processes. Interestingly, besides the role of tissue material properties in determining how much tissues deform in response to force application, there is increasing theoretical and experimental evidence, suggesting that tissue material properties can abruptly and drastically change in development. These changes resemble phase transitions, pointing at the intriguing possibility that important morphogenetic processes in development, such as symmetry breaking and self‐organization, might be mediated by tissue phase transitions. In this review, we summarize recent findings on the regulation and role of tissue material properties in the context of the developing embryo. We posit that abrupt changes of tissue rheological properties may have important implications in maintaining the balance between robustness and adaptability during embryonic development.","lang":"eng"}],"issue":"20","isi":1,"quality_controlled":"1","project":[{"grant_number":"742573","_id":"260F1432-B435-11E9-9278-68D0E5697425","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","call_identifier":"H2020"},{"_id":"2693FD8C-B435-11E9-9278-68D0E5697425","grant_number":"V00736","name":"Tissue material properties in embryonic development","call_identifier":"FWF"}],"external_id":{"pmid":["31512749"],"isi":["000485561900001"]},"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"language":[{"iso":"eng"}],"doi":"10.15252/embj.2019102497","month":"10","publication_identifier":{"issn":["0261-4189"],"eissn":["1460-2075"]},"publication_status":"published","department":[{"_id":"CaHe"}],"publisher":"EMBO","year":"2019","pmid":1,"date_updated":"2023-09-05T13:04:13Z","date_created":"2019-11-04T15:24:29Z","volume":38,"author":[{"full_name":"Petridou, Nicoletta","last_name":"Petridou","first_name":"Nicoletta","orcid":"0000-0002-8451-1195","id":"2A003F6C-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"}],"article_number":"e102497","file_date_updated":"2020-07-14T12:47:46Z","ec_funded":1},{"file_date_updated":"2020-07-14T12:47:46Z","date_updated":"2023-09-05T15:01:12Z","date_created":"2019-11-04T16:20:19Z","volume":68,"author":[{"last_name":"McDougall","first_name":"Alex","full_name":"McDougall, Alex"},{"full_name":"Chenevert, Janet","last_name":"Chenevert","first_name":"Janet"},{"full_name":"Godard, Benoit G","last_name":"Godard","first_name":"Benoit G","id":"33280250-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Dumollard, Remi","last_name":"Dumollard","first_name":"Remi"}],"publication_status":"published","editor":[{"full_name":"Tworzydlo, Waclaw","last_name":"Tworzydlo","first_name":"Waclaw"},{"full_name":"Bilinski, Szczepan M.","first_name":"Szczepan M.","last_name":"Bilinski"}],"publisher":"Springer Nature","department":[{"_id":"CaHe"}],"year":"2019","pmid":1,"month":"10","publication_identifier":{"isbn":["9783030234584","9783030234591"],"eissn":["1861-0412"],"issn":["0080-1844"]},"language":[{"iso":"eng"}],"doi":"10.1007/978-3-030-23459-1_6","quality_controlled":"1","oa":1,"external_id":{"pmid":["31598855"]},"abstract":[{"text":"Cells are arranged into species-specific patterns during early embryogenesis. Such cell division patterns are important since they often reflect the distribution of localized cortical factors from eggs/fertilized eggs to specific cells as well as the emergence of organismal form. However, it has proven difficult to reveal the mechanisms that underlie the emergence of cell positioning patterns that underlie embryonic shape, likely because a systems-level approach is required that integrates cell biological, genetic, developmental, and mechanical parameters. The choice of organism to address such questions is also important. Because ascidians display the most extreme form of invariant cleavage pattern among the metazoans, we have been analyzing the cell biological mechanisms that underpin three aspects of cell division (unequal cell division (UCD), oriented cell division (OCD), and asynchronous cell cycles) which affect the overall shape of the blastula-stage ascidian embryo composed of 64 cells. In ascidians, UCD creates two small cells at the 16-cell stage that in turn undergo two further successive rounds of UCD. Starting at the 16-cell stage, the cell cycle becomes asynchronous, whereby the vegetal half divides before the animal half, thus creating 24-, 32-, 44-, and then 64-cell stages. Perturbing either UCD or the alternate cell division rhythm perturbs cell position. We propose that dynamic cell shape changes propagate throughout the embryo via cell-cell contacts to create the ascidian-specific invariant cleavage pattern.","lang":"eng"}],"alternative_title":["RESULTS"],"type":"book_chapter","oa_version":"Submitted Version","file":[{"checksum":"7f43e1e3706d15061475c5c57efc2786","date_created":"2020-05-14T10:09:30Z","date_updated":"2020-07-14T12:47:46Z","relation":"main_file","file_id":"7829","file_size":19317348,"content_type":"application/pdf","creator":"dernst","access_level":"open_access","file_name":"2019_RESULTS_McDougall.pdf"}],"title":"Emergence of embryo shape during cleavage divisions","status":"public","ddc":["570"],"intvolume":" 68","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","_id":"6987","day":"10","has_accepted_license":"1","article_processing_charge":"No","scopus_import":"1","date_published":"2019-10-10T00:00:00Z","page":"127-154","publication":"Evo-Devo: Non-model species in cell and developmental biology","citation":{"mla":"McDougall, Alex, et al. “Emergence of Embryo Shape during Cleavage Divisions.” Evo-Devo: Non-Model Species in Cell and Developmental Biology, edited by Waclaw Tworzydlo and Szczepan M. Bilinski, vol. 68, Springer Nature, 2019, pp. 127–54, doi:10.1007/978-3-030-23459-1_6.","short":"A. McDougall, J. Chenevert, B.G. Godard, R. Dumollard, in:, W. Tworzydlo, S.M. Bilinski (Eds.), Evo-Devo: Non-Model Species in Cell and Developmental Biology, Springer Nature, 2019, pp. 127–154.","chicago":"McDougall, Alex, Janet Chenevert, Benoit G Godard, and Remi Dumollard. “Emergence of Embryo Shape during Cleavage Divisions.” In Evo-Devo: Non-Model Species in Cell and Developmental Biology, edited by Waclaw Tworzydlo and Szczepan M. Bilinski, 68:127–54. Springer Nature, 2019. https://doi.org/10.1007/978-3-030-23459-1_6.","ama":"McDougall A, Chenevert J, Godard BG, Dumollard R. Emergence of embryo shape during cleavage divisions. In: Tworzydlo W, Bilinski SM, eds. Evo-Devo: Non-Model Species in Cell and Developmental Biology. Vol 68. Springer Nature; 2019:127-154. doi:10.1007/978-3-030-23459-1_6","ista":"McDougall A, Chenevert J, Godard BG, Dumollard R. 2019.Emergence of embryo shape during cleavage divisions. In: Evo-Devo: Non-model species in cell and developmental biology. RESULTS, vol. 68, 127–154.","apa":"McDougall, A., Chenevert, J., Godard, B. G., & Dumollard, R. (2019). Emergence of embryo shape during cleavage divisions. In W. Tworzydlo & S. M. Bilinski (Eds.), Evo-Devo: Non-model species in cell and developmental biology (Vol. 68, pp. 127–154). Springer Nature. https://doi.org/10.1007/978-3-030-23459-1_6","ieee":"A. McDougall, J. Chenevert, B. G. Godard, and R. Dumollard, “Emergence of embryo shape during cleavage divisions,” in Evo-Devo: Non-model species in cell and developmental biology, vol. 68, W. Tworzydlo and S. M. Bilinski, Eds. Springer Nature, 2019, pp. 127–154."}},{"file_date_updated":"2020-07-14T12:47:52Z","author":[{"full_name":"Schwayer, Cornelia","last_name":"Schwayer","first_name":"Cornelia","orcid":"0000-0001-5130-2226","id":"3436488C-F248-11E8-B48F-1D18A9856A87"}],"related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"1096"},{"relation":"part_of_dissertation","status":"public","id":"7001"}]},"date_updated":"2023-09-07T12:56:42Z","date_created":"2019-12-16T14:26:14Z","year":"2019","publication_status":"published","department":[{"_id":"CaHe"}],"publisher":"Institute of Science and Technology Austria","month":"12","publication_identifier":{"issn":["2663-337X"]},"doi":"10.15479/AT:ISTA:7186","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"EM-Fac"},{"_id":"SSU"}],"degree_awarded":"PhD","supervisor":[{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg"}],"language":[{"iso":"eng"}],"oa":1,"abstract":[{"lang":"eng","text":"Tissue morphogenesis in developmental or physiological processes is regulated by molecular\r\nand mechanical signals. While the molecular signaling cascades are increasingly well\r\ndescribed, the mechanical signals affecting tissue shape changes have only recently been\r\nstudied in greater detail. To gain more insight into the mechanochemical and biophysical\r\nbasis of an epithelial spreading process (epiboly) in early zebrafish development, we studied\r\ncell-cell junction formation and actomyosin network dynamics at the boundary between\r\nsurface layer epithelial cells (EVL) and the yolk syncytial layer (YSL). During zebrafish epiboly,\r\nthe cell mass sitting on top of the yolk cell spreads to engulf the yolk cell by the end of\r\ngastrulation. It has been previously shown that an actomyosin ring residing within the YSL\r\npulls on the EVL tissue through a cable-constriction and a flow-friction motor, thereby\r\ndragging the tissue vegetal wards. Pulling forces are likely transmitted from the YSL\r\nactomyosin ring to EVL cells; however, the nature and formation of the junctional structure\r\nmediating this process has not been well described so far. Therefore, our main aim was to\r\ndetermine the nature, dynamics and potential function of the EVL-YSL junction during this\r\nepithelial tissue spreading. Specifically, we show that the EVL-YSL junction is a\r\nmechanosensitive structure, predominantly made of tight junction (TJ) proteins. The process\r\nof TJ mechanosensation depends on the retrograde flow of non-junctional, phase-separated\r\nZonula Occludens-1 (ZO-1) protein clusters towards the EVL-YSL boundary. Interestingly, we\r\ncould demonstrate that ZO-1 is present in a non-junctional pool on the surface of the yolk\r\ncell, and ZO-1 undergoes a phase separation process that likely renders the protein\r\nresponsive to flows. These flows are directed towards the junction and mediate proper\r\ntension-dependent recruitment of ZO-1. Upon reaching the EVL-YSL junction ZO-1 gets\r\nincorporated into the junctional pool mediated through its direct actin-binding domain.\r\nWhen the non-junctional pool and/or ZO-1 direct actin binding is absent, TJs fail in their\r\nproper mechanosensitive responses resulting in slower tissue spreading. We could further\r\ndemonstrate that depletion of ZO proteins within the YSL results in diminished actomyosin\r\nring formation. This suggests that a mechanochemical feedback loop is at work during\r\nzebrafish epiboly: ZO proteins help in proper actomyosin ring formation and actomyosin\r\ncontractility and flows positively influence ZO-1 junctional recruitment. Finally, such a\r\nmesoscale polarization process mediated through the flow of phase-separated protein\r\nclusters might have implications for other processes such as immunological synapse\r\nformation, C. elegans zygote polarization and wound healing."}],"type":"dissertation","alternative_title":["ISTA Thesis"],"file":[{"access_level":"closed","file_name":"DocumentSourceFiles.zip","creator":"cschwayer","content_type":"application/zip","file_size":19431292,"file_id":"7194","relation":"source_file","checksum":"585583c1c875c5d9525703a539668a7c","date_updated":"2020-07-14T12:47:52Z","date_created":"2019-12-19T15:18:11Z"},{"relation":"main_file","file_id":"7195","checksum":"9b9b24351514948d27cec659e632e2cd","date_created":"2019-12-19T15:19:21Z","date_updated":"2020-07-14T12:47:52Z","access_level":"open_access","file_name":"Thesis_CS_final.pdf","file_size":19226428,"content_type":"application/pdf","creator":"cschwayer"}],"oa_version":"Published Version","_id":"7186","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","ddc":["570"],"status":"public","title":"Mechanosensation of tight junctions depends on ZO-1 phase separation and flow","day":"16","has_accepted_license":"1","article_processing_charge":"No","date_published":"2019-12-16T00:00:00Z","citation":{"chicago":"Schwayer, Cornelia. “Mechanosensation of Tight Junctions Depends on ZO-1 Phase Separation and Flow.” Institute of Science and Technology Austria, 2019. https://doi.org/10.15479/AT:ISTA:7186.","mla":"Schwayer, Cornelia. Mechanosensation of Tight Junctions Depends on ZO-1 Phase Separation and Flow. Institute of Science and Technology Austria, 2019, doi:10.15479/AT:ISTA:7186.","short":"C. Schwayer, Mechanosensation of Tight Junctions Depends on ZO-1 Phase Separation and Flow, Institute of Science and Technology Austria, 2019.","ista":"Schwayer C. 2019. Mechanosensation of tight junctions depends on ZO-1 phase separation and flow. Institute of Science and Technology Austria.","apa":"Schwayer, C. (2019). Mechanosensation of tight junctions depends on ZO-1 phase separation and flow. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:7186","ieee":"C. Schwayer, “Mechanosensation of tight junctions depends on ZO-1 phase separation and flow,” Institute of Science and Technology Austria, 2019.","ama":"Schwayer C. Mechanosensation of tight junctions depends on ZO-1 phase separation and flow. 2019. doi:10.15479/AT:ISTA:7186"},"page":"107"},{"_id":"5789","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","status":"public","ddc":["570"],"title":"Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling","intvolume":" 21","file":[{"file_name":"2018_NatureCellBio_Petridou_accepted.pdf","access_level":"open_access","creator":"dernst","file_size":71590590,"content_type":"application/pdf","file_id":"8685","relation":"main_file","date_created":"2020-10-21T07:18:35Z","date_updated":"2020-10-21T07:18:35Z","success":1,"checksum":"e38523787b3bc84006f2793de99ad70f"}],"oa_version":"Submitted Version","type":"journal_article","abstract":[{"lang":"eng","text":"Tissue morphogenesis is driven by mechanical forces that elicit changes in cell size, shape and motion. The extent by which forces deform tissues critically depends on the rheological properties of the recipient tissue. Yet, whether and how dynamic changes in tissue rheology affect tissue morphogenesis and how they are regulated within the developing organism remain unclear. Here, we show that blastoderm spreading at the onset of zebrafish morphogenesis relies on a rapid, pronounced and spatially patterned tissue fluidization. Blastoderm fluidization is temporally controlled by mitotic cell rounding-dependent cell–cell contact disassembly during the last rounds of cell cleavages. Moreover, fluidization is spatially restricted to the central blastoderm by local activation of non-canonical Wnt signalling within the blastoderm margin, increasing cell cohesion and thereby counteracting the effect of mitotic rounding on contact disassembly. Overall, our results identify a fluidity transition mediated by loss of cell cohesion as a critical regulator of embryo morphogenesis."}],"publication":"Nature Cell Biology","citation":{"chicago":"Petridou, Nicoletta, Silvia Grigolon, Guillaume Salbreux, Edouard B Hannezo, and Carl-Philipp J Heisenberg. “Fluidization-Mediated Tissue Spreading by Mitotic Cell Rounding and Non-Canonical Wnt Signalling.” Nature Cell Biology. Nature Publishing Group, 2019. https://doi.org/10.1038/s41556-018-0247-4.","mla":"Petridou, Nicoletta, et al. “Fluidization-Mediated Tissue Spreading by Mitotic Cell Rounding and Non-Canonical Wnt Signalling.” Nature Cell Biology, vol. 21, Nature Publishing Group, 2019, pp. 169–178, doi:10.1038/s41556-018-0247-4.","short":"N. Petridou, S. Grigolon, G. Salbreux, E.B. Hannezo, C.-P.J. Heisenberg, Nature Cell Biology 21 (2019) 169–178.","ista":"Petridou N, Grigolon S, Salbreux G, Hannezo EB, Heisenberg C-PJ. 2019. Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling. Nature Cell Biology. 21, 169–178.","ieee":"N. Petridou, S. Grigolon, G. Salbreux, E. B. Hannezo, and C.-P. J. Heisenberg, “Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling,” Nature Cell Biology, vol. 21. Nature Publishing Group, pp. 169–178, 2019.","apa":"Petridou, N., Grigolon, S., Salbreux, G., Hannezo, E. B., & Heisenberg, C.-P. J. (2019). Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling. Nature Cell Biology. Nature Publishing Group. https://doi.org/10.1038/s41556-018-0247-4","ama":"Petridou N, Grigolon S, Salbreux G, Hannezo EB, Heisenberg C-PJ. Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling. Nature Cell Biology. 2019;21:169–178. doi:10.1038/s41556-018-0247-4"},"article_type":"original","page":"169–178","date_published":"2019-02-01T00:00:00Z","scopus_import":"1","day":"01","has_accepted_license":"1","article_processing_charge":"No","year":"2019","pmid":1,"publication_status":"published","publisher":"Nature Publishing Group","department":[{"_id":"CaHe"},{"_id":"EdHa"}],"author":[{"full_name":"Petridou, Nicoletta","id":"2A003F6C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8451-1195","first_name":"Nicoletta","last_name":"Petridou"},{"full_name":"Grigolon, Silvia","first_name":"Silvia","last_name":"Grigolon"},{"full_name":"Salbreux, Guillaume","first_name":"Guillaume","last_name":"Salbreux"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561","first_name":"Edouard B","last_name":"Hannezo","full_name":"Hannezo, Edouard B"},{"first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J"}],"related_material":{"link":[{"relation":"press_release","description":"News on IST Homepage","url":"https://ist.ac.at/en/news/when-a-fish-becomes-fluid/"}]},"date_created":"2018-12-30T22:59:15Z","date_updated":"2023-09-11T14:03:28Z","volume":21,"file_date_updated":"2020-10-21T07:18:35Z","ec_funded":1,"oa":1,"external_id":{"pmid":["30559456"],"isi":["000457468300011"]},"quality_controlled":"1","isi":1,"project":[{"call_identifier":"H2020","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","grant_number":"742573","_id":"260F1432-B435-11E9-9278-68D0E5697425"},{"name":"Molecular mechanism of auxindriven formative divisions delineating lateral root organogenesis in plants (EMBO fellowship)","grant_number":"ALTF710-2016","_id":"253E54C8-B435-11E9-9278-68D0E5697425"}],"doi":"10.1038/s41556-018-0247-4","acknowledged_ssus":[{"_id":"Bio"}],"language":[{"iso":"eng"}],"month":"02","publication_identifier":{"issn":["14657392"]}},{"volume":177,"date_created":"2019-06-02T21:59:12Z","date_updated":"2024-03-28T23:30:39Z","related_material":{"link":[{"description":"News on IST Homepage","relation":"press_release","url":"https://ist.ac.at/en/news/how-the-cytoplasm-separates-from-the-yolk/"}],"record":[{"id":"8350","status":"public","relation":"dissertation_contains"}]},"author":[{"id":"40B34FE2-F248-11E8-B48F-1D18A9856A87","first_name":"Shayan","last_name":"Shamipour","full_name":"Shamipour, Shayan"},{"last_name":"Kardos","first_name":"Roland","id":"4039350E-F248-11E8-B48F-1D18A9856A87","full_name":"Kardos, Roland"},{"full_name":"Xue, Shi-lei","id":"31D2C804-F248-11E8-B48F-1D18A9856A87","first_name":"Shi-lei","last_name":"Xue"},{"full_name":"Hof, Björn","last_name":"Hof","first_name":"Björn","orcid":"0000-0003-2057-2754","id":"3A374330-F248-11E8-B48F-1D18A9856A87"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561","first_name":"Edouard B","last_name":"Hannezo","full_name":"Hannezo, Edouard B"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"department":[{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"BjHo"}],"publisher":"Elsevier","publication_status":"published","pmid":1,"acknowledgement":"We would like to thank Pierre Recho, Guillaume Salbreux, and Silvia Grigolon for advice on the theory, Lila Solnica-Krezel for kindly providing us with zebrafish dachsous mutants, members of the Heisenberg and Hannezo groups for fruitful discussions, and the Bioimaging and zebrafish facilities at IST Austria for their continuous support. This project has received funding from the European Union (European Research Council Advanced Grant 742573 to C.P.H.) and from the Austrian Science Fund (FWF) (P 31639 to E.H.).","year":"2019","ec_funded":1,"file_date_updated":"2020-10-21T07:22:34Z","language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"}],"doi":"10.1016/j.cell.2019.04.030","project":[{"grant_number":"742573","_id":"260F1432-B435-11E9-9278-68D0E5697425","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","call_identifier":"H2020"},{"call_identifier":"FWF","name":"Active mechano-chemical description of the cell cytoskeleton","_id":"268294B6-B435-11E9-9278-68D0E5697425","grant_number":"P31639"}],"isi":1,"quality_controlled":"1","oa":1,"external_id":{"isi":["000469415100013"],"pmid":["31080065"]},"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1016/j.cell.2019.04.030"}],"publication_identifier":{"eissn":["10974172"],"issn":["00928674"]},"month":"05","file":[{"content_type":"application/pdf","file_size":3356292,"creator":"dernst","file_name":"2019_Cell_Shamipour_accepted.pdf","access_level":"open_access","date_created":"2020-10-21T07:22:34Z","date_updated":"2020-10-21T07:22:34Z","checksum":"aea43726d80e35ce3885073a5f05c3e3","success":1,"relation":"main_file","file_id":"8686"}],"oa_version":"Published Version","intvolume":" 177","ddc":["570"],"title":"Bulk actin dynamics drive phase segregation in zebrafish oocytes","status":"public","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"6508","issue":"6","abstract":[{"text":"Segregation of maternal determinants within the oocyte constitutes the first step in embryo patterning. In zebrafish oocytes, extensive ooplasmic streaming leads to the segregation of ooplasm from yolk granules along the animal-vegetal axis of the oocyte. Here, we show that this process does not rely on cortical actin reorganization, as previously thought, but instead on a cell-cycle-dependent bulk actin polymerization wave traveling from the animal to the vegetal pole of the oocyte. This wave functions in segregation by both pulling ooplasm animally and pushing yolk granules vegetally. Using biophysical experimentation and theory, we show that ooplasm pulling is mediated by bulk actin network flows exerting friction forces on the ooplasm, while yolk granule pushing is achieved by a mechanism closely resembling actin comet formation on yolk granules. Our study defines a novel role of cell-cycle-controlled bulk actin polymerization waves in oocyte polarization via ooplasmic segregation.","lang":"eng"}],"type":"journal_article","date_published":"2019-05-30T00:00:00Z","page":"1463-1479.e18","article_type":"original","citation":{"short":"S. Shamipour, R. Kardos, S. Xue, B. Hof, E.B. Hannezo, C.-P.J. Heisenberg, Cell 177 (2019) 1463–1479.e18.","mla":"Shamipour, Shayan, et al. “Bulk Actin Dynamics Drive Phase Segregation in Zebrafish Oocytes.” Cell, vol. 177, no. 6, Elsevier, 2019, p. 1463–1479.e18, doi:10.1016/j.cell.2019.04.030.","chicago":"Shamipour, Shayan, Roland Kardos, Shi-lei Xue, Björn Hof, Edouard B Hannezo, and Carl-Philipp J Heisenberg. “Bulk Actin Dynamics Drive Phase Segregation in Zebrafish Oocytes.” Cell. Elsevier, 2019. https://doi.org/10.1016/j.cell.2019.04.030.","ama":"Shamipour S, Kardos R, Xue S, Hof B, Hannezo EB, Heisenberg C-PJ. Bulk actin dynamics drive phase segregation in zebrafish oocytes. Cell. 2019;177(6):1463-1479.e18. doi:10.1016/j.cell.2019.04.030","ieee":"S. Shamipour, R. Kardos, S. Xue, B. Hof, E. B. Hannezo, and C.-P. J. Heisenberg, “Bulk actin dynamics drive phase segregation in zebrafish oocytes,” Cell, vol. 177, no. 6. Elsevier, p. 1463–1479.e18, 2019.","apa":"Shamipour, S., Kardos, R., Xue, S., Hof, B., Hannezo, E. B., & Heisenberg, C.-P. J. (2019). Bulk actin dynamics drive phase segregation in zebrafish oocytes. Cell. Elsevier. https://doi.org/10.1016/j.cell.2019.04.030","ista":"Shamipour S, Kardos R, Xue S, Hof B, Hannezo EB, Heisenberg C-PJ. 2019. Bulk actin dynamics drive phase segregation in zebrafish oocytes. Cell. 177(6), 1463–1479.e18."},"publication":"Cell","article_processing_charge":"No","has_accepted_license":"1","day":"30","scopus_import":"1"},{"date_published":"2019-10-31T00:00:00Z","citation":{"apa":"Schwayer, C., Shamipour, S., Pranjic-Ferscha, K., Schauer, A., Balda, M., Tada, M., … Heisenberg, C.-P. J. (2019). Mechanosensation of tight junctions depends on ZO-1 phase separation and flow. Cell. Cell Press. https://doi.org/10.1016/j.cell.2019.10.006","ieee":"C. Schwayer et al., “Mechanosensation of tight junctions depends on ZO-1 phase separation and flow,” Cell, vol. 179, no. 4. Cell Press, p. 937–952.e18, 2019.","ista":"Schwayer C, Shamipour S, Pranjic-Ferscha K, Schauer A, Balda M, Tada M, Matter K, Heisenberg C-PJ. 2019. Mechanosensation of tight junctions depends on ZO-1 phase separation and flow. Cell. 179(4), 937–952.e18.","ama":"Schwayer C, Shamipour S, Pranjic-Ferscha K, et al. Mechanosensation of tight junctions depends on ZO-1 phase separation and flow. Cell. 2019;179(4):937-952.e18. doi:10.1016/j.cell.2019.10.006","chicago":"Schwayer, Cornelia, Shayan Shamipour, Kornelija Pranjic-Ferscha, Alexandra Schauer, M Balda, M Tada, K Matter, and Carl-Philipp J Heisenberg. “Mechanosensation of Tight Junctions Depends on ZO-1 Phase Separation and Flow.” Cell. Cell Press, 2019. https://doi.org/10.1016/j.cell.2019.10.006.","short":"C. Schwayer, S. Shamipour, K. Pranjic-Ferscha, A. Schauer, M. Balda, M. Tada, K. Matter, C.-P.J. Heisenberg, Cell 179 (2019) 937–952.e18.","mla":"Schwayer, Cornelia, et al. “Mechanosensation of Tight Junctions Depends on ZO-1 Phase Separation and Flow.” Cell, vol. 179, no. 4, Cell Press, 2019, p. 937–952.e18, doi:10.1016/j.cell.2019.10.006."},"publication":"Cell","page":"937-952.e18","article_type":"original","has_accepted_license":"1","article_processing_charge":"No","day":"31","scopus_import":"1","oa_version":"Submitted Version","file":[{"file_name":"2019_Cell_Schwayer_accepted.pdf","access_level":"open_access","creator":"dernst","file_size":8805878,"content_type":"application/pdf","file_id":"8684","relation":"main_file","date_updated":"2020-10-21T07:09:45Z","date_created":"2020-10-21T07:09:45Z","success":1,"checksum":"33dac4bb77ee630e2666e936b4d57980"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"7001","intvolume":" 179","ddc":["570"],"status":"public","title":"Mechanosensation of tight junctions depends on ZO-1 phase separation and flow","issue":"4","type":"journal_article","doi":"10.1016/j.cell.2019.10.006","language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"}],"oa":1,"external_id":{"pmid":["31675500"],"isi":["000493898000012"]},"project":[{"_id":"260F1432-B435-11E9-9278-68D0E5697425","grant_number":"742573","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","call_identifier":"H2020"}],"quality_controlled":"1","isi":1,"publication_identifier":{"eissn":["1097-4172"],"issn":["0092-8674"]},"month":"10","related_material":{"record":[{"id":"7186","status":"public","relation":"dissertation_contains"},{"status":"public","relation":"dissertation_contains","id":"8350"}],"link":[{"url":"https://ist.ac.at/en/news/biochemistry-meets-mechanics-the-sensitive-nature-of-cell-cell-contact-formation-in-embryo-development/","description":"News auf IST Website","relation":"press_release"}]},"author":[{"full_name":"Schwayer, Cornelia","first_name":"Cornelia","last_name":"Schwayer","id":"3436488C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5130-2226"},{"full_name":"Shamipour, Shayan","first_name":"Shayan","last_name":"Shamipour","id":"40B34FE2-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Kornelija","last_name":"Pranjic-Ferscha","id":"4362B3C2-F248-11E8-B48F-1D18A9856A87","full_name":"Pranjic-Ferscha, Kornelija"},{"orcid":"0000-0001-7659-9142","id":"30A536BA-F248-11E8-B48F-1D18A9856A87","last_name":"Schauer","first_name":"Alexandra","full_name":"Schauer, Alexandra"},{"last_name":"Balda","first_name":"M","full_name":"Balda, M"},{"first_name":"M","last_name":"Tada","full_name":"Tada, M"},{"full_name":"Matter, K","first_name":"K","last_name":"Matter"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"volume":179,"date_updated":"2024-03-28T23:30:39Z","date_created":"2019-11-12T12:51:06Z","pmid":1,"year":"2019","department":[{"_id":"CaHe"},{"_id":"BjHo"}],"publisher":"Cell Press","publication_status":"published","ec_funded":1,"file_date_updated":"2020-10-21T07:09:45Z"},{"volume":45,"date_updated":"2023-09-11T13:22:13Z","date_created":"2018-12-11T11:45:44Z","related_material":{"link":[{"url":"https://ist.ac.at/en/news/cells-change-tension-to-make-tissue-barriers-easier-to-get-through/","relation":"press_release","description":"News on IST Homepage"}]},"author":[{"last_name":"Ratheesh","first_name":"Aparna","orcid":"0000-0001-7190-0776","id":"2F064CFE-F248-11E8-B48F-1D18A9856A87","full_name":"Ratheesh, Aparna"},{"id":"3CCBB46E-F248-11E8-B48F-1D18A9856A87","first_name":"Julia","last_name":"Biebl","full_name":"Biebl, Julia"},{"full_name":"Smutny, Michael","last_name":"Smutny","first_name":"Michael"},{"full_name":"Veselá, Jana","last_name":"Veselá","first_name":"Jana","id":"433253EE-F248-11E8-B48F-1D18A9856A87"},{"id":"41DB591E-F248-11E8-B48F-1D18A9856A87","last_name":"Papusheva","first_name":"Ekaterina","full_name":"Papusheva, Ekaterina"},{"full_name":"Krens, Gabriel","orcid":"0000-0003-4761-5996","id":"2B819732-F248-11E8-B48F-1D18A9856A87","last_name":"Krens","first_name":"Gabriel"},{"full_name":"Kaufmann, Walter","last_name":"Kaufmann","first_name":"Walter","orcid":"0000-0001-9735-5315","id":"3F99E422-F248-11E8-B48F-1D18A9856A87"},{"full_name":"György, Attila","last_name":"György","first_name":"Attila","orcid":"0000-0002-1819-198X","id":"3BCEDBE0-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Casano","first_name":"Alessandra M","orcid":"0000-0002-6009-6804","id":"3DBA3F4E-F248-11E8-B48F-1D18A9856A87","full_name":"Casano, Alessandra M"},{"orcid":"0000-0001-8323-8353","id":"3D224B9E-F248-11E8-B48F-1D18A9856A87","last_name":"Siekhaus","first_name":"Daria E","full_name":"Siekhaus, Daria E"}],"publisher":"Elsevier","department":[{"_id":"DaSi"},{"_id":"CaHe"},{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"MiSi"}],"publication_status":"published","pmid":1,"year":"2018","ec_funded":1,"language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"SSU"}],"doi":"10.1016/j.devcel.2018.04.002","project":[{"grant_number":"P29638","_id":"253B6E48-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Drosophila TNFa´s Funktion in Immunzellen"},{"_id":"2536F660-B435-11E9-9278-68D0E5697425","grant_number":"334077","name":"Investigating the role of transporters in invasive migration through junctions","call_identifier":"FP7"}],"isi":1,"quality_controlled":"1","external_id":{"pmid":["29738712"],"isi":["000432461400009"]},"oa":1,"main_file_link":[{"url":"https://doi.org/10.1016/j.devcel.2018.04.002","open_access":"1"}],"month":"05","oa_version":"Published Version","intvolume":" 45","title":"Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage invasive migration","status":"public","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","_id":"308","issue":"3","abstract":[{"lang":"eng","text":"Migrating cells penetrate tissue barriers during development, inflammatory responses, and tumor metastasis. We study if migration in vivo in such three-dimensionally confined environments requires changes in the mechanical properties of the surrounding cells using embryonic Drosophila melanogaster hemocytes, also called macrophages, as a model. We find that macrophage invasion into the germband through transient separation of the apposing ectoderm and mesoderm requires cell deformations and reductions in apical tension in the ectoderm. Interestingly, the genetic pathway governing these mechanical shifts acts downstream of the only known tumor necrosis factor superfamily member in Drosophila, Eiger, and its receptor, Grindelwald. Eiger-Grindelwald signaling reduces levels of active Myosin in the germband ectodermal cortex through the localization of a Crumbs complex component, Patj (Pals-1-associated tight junction protein). We therefore elucidate a distinct molecular pathway that controls tissue tension and demonstrate the importance of such regulation for invasive migration in vivo."}],"type":"journal_article","date_published":"2018-05-07T00:00:00Z","page":"331 - 346","article_type":"original","citation":{"chicago":"Ratheesh, Aparna, Julia Bicher, Michael Smutny, Jana Veselá, Ekaterina Papusheva, Gabriel Krens, Walter Kaufmann, Attila György, Alessandra M Casano, and Daria E Siekhaus. “Drosophila TNF Modulates Tissue Tension in the Embryo to Facilitate Macrophage Invasive Migration.” Developmental Cell. Elsevier, 2018. https://doi.org/10.1016/j.devcel.2018.04.002.","mla":"Ratheesh, Aparna, et al. “Drosophila TNF Modulates Tissue Tension in the Embryo to Facilitate Macrophage Invasive Migration.” Developmental Cell, vol. 45, no. 3, Elsevier, 2018, pp. 331–46, doi:10.1016/j.devcel.2018.04.002.","short":"A. Ratheesh, J. Bicher, M. Smutny, J. Veselá, E. Papusheva, G. Krens, W. Kaufmann, A. György, A.M. Casano, D.E. Siekhaus, Developmental Cell 45 (2018) 331–346.","ista":"Ratheesh A, Bicher J, Smutny M, Veselá J, Papusheva E, Krens G, Kaufmann W, György A, Casano AM, Siekhaus DE. 2018. Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage invasive migration. Developmental Cell. 45(3), 331–346.","apa":"Ratheesh, A., Bicher, J., Smutny, M., Veselá, J., Papusheva, E., Krens, G., … Siekhaus, D. E. (2018). Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage invasive migration. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2018.04.002","ieee":"A. Ratheesh et al., “Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage invasive migration,” Developmental Cell, vol. 45, no. 3. Elsevier, pp. 331–346, 2018.","ama":"Ratheesh A, Bicher J, Smutny M, et al. Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage invasive migration. Developmental Cell. 2018;45(3):331-346. doi:10.1016/j.devcel.2018.04.002"},"publication":"Developmental Cell","article_processing_charge":"No","day":"07","scopus_import":"1"},{"year":"2018","acknowledgement":"Research in the Bellaïche laboratory is supported by the European Research Council (ERC Advanced, TiMoprh, 340784), the Fondation ARC pour la Recherche sur le Cancer (SL220130607097), the Agence Nationale de la Recherche (ANR lLabex DEEP; 11-LBX-0044, ANR-10-IDEX-0001-02), the Centre National de la Recherche Scientifique, the Institut National de la Santé et de la Recherche Médicale, and Institut Curie and PSL Research University funding or grants.","publication_status":"published","department":[{"_id":"CaHe"}],"publisher":"Cell Press","author":[{"id":"2E839F16-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4333-7503","first_name":"Diana C","last_name":"Nunes Pinheiro","full_name":"Nunes Pinheiro, Diana C"},{"first_name":"Yohanns","last_name":"Bellaïche","full_name":"Bellaïche, Yohanns"}],"date_created":"2018-12-11T11:44:23Z","date_updated":"2023-09-13T08:54:38Z","volume":47,"publist_id":"8000","main_file_link":[{"url":"https://doi.org/10.1016/j.devcel.2018.09.014"}],"external_id":{"isi":["000446579900002"]},"isi":1,"quality_controlled":"1","doi":"10.1016/j.devcel.2018.09.014","language":[{"iso":"eng"}],"month":"10","_id":"54","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","status":"public","title":"Mechanical force-driven adherents junction remodeling and epithelial dynamics","intvolume":" 47","oa_version":"Published Version","type":"journal_article","abstract":[{"lang":"eng","text":"During epithelial tissue development, repair, and homeostasis, adherens junctions (AJs) ensure intercellular adhesion and tissue integrity while allowing for cell and tissue dynamics. Mechanical forces play critical roles in AJs’ composition and dynamics. Recent findings highlight that beyond a well-established role in reinforcing cell-cell adhesion, AJ mechanosensitivity promotes junctional remodeling and polarization, thereby regulating critical processes such as cell intercalation, division, and collective migration. Here, we provide an integrated view of mechanosensing mechanisms that regulate cell-cell contact composition, geometry, and integrity under tension and highlight pivotal roles for mechanosensitive AJ remodeling in preserving epithelial integrity and sustaining tissue dynamics."}],"issue":"1","publication":"Developmental Cell","citation":{"mla":"Nunes Pinheiro, Diana C., and Yohanns Bellaïche. “Mechanical Force-Driven Adherents Junction Remodeling and Epithelial Dynamics.” Developmental Cell, vol. 47, no. 1, Cell Press, 2018, pp. 3–19, doi:10.1016/j.devcel.2018.09.014.","short":"D.C. Nunes Pinheiro, Y. Bellaïche, Developmental Cell 47 (2018) 3–19.","chicago":"Nunes Pinheiro, Diana C, and Yohanns Bellaïche. “Mechanical Force-Driven Adherents Junction Remodeling and Epithelial Dynamics.” Developmental Cell. Cell Press, 2018. https://doi.org/10.1016/j.devcel.2018.09.014.","ama":"Nunes Pinheiro DC, Bellaïche Y. Mechanical force-driven adherents junction remodeling and epithelial dynamics. Developmental Cell. 2018;47(1):3-19. doi:10.1016/j.devcel.2018.09.014","ista":"Nunes Pinheiro DC, Bellaïche Y. 2018. Mechanical force-driven adherents junction remodeling and epithelial dynamics. Developmental Cell. 47(1), 3–19.","ieee":"D. C. Nunes Pinheiro and Y. Bellaïche, “Mechanical force-driven adherents junction remodeling and epithelial dynamics,” Developmental Cell, vol. 47, no. 1. Cell Press, pp. 3–19, 2018.","apa":"Nunes Pinheiro, D. C., & Bellaïche, Y. (2018). Mechanical force-driven adherents junction remodeling and epithelial dynamics. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2018.09.014"},"article_type":"review","page":"3 - 19","date_published":"2018-10-08T00:00:00Z","scopus_import":"1","day":"08","article_processing_charge":"No"},{"article_processing_charge":"No","day":"01","scopus_import":"1","date_published":"2018-12-01T00:00:00Z","page":"4267-4283","citation":{"short":"L. Carvalho, P. Patricio, S. Ponte, C.-P.J. Heisenberg, L. Almeida, A.S. Nunes, N.A.M. Araújo, A. Jacinto, Journal of Cell Biology 217 (2018) 4267–4283.","mla":"Carvalho, Lara, et al. “Occluding Junctions as Novel Regulators of Tissue Mechanics during Wound Repair.” Journal of Cell Biology, vol. 217, no. 12, Rockefeller University Press, 2018, pp. 4267–83, doi:10.1083/jcb.201804048.","chicago":"Carvalho, Lara, Pedro Patricio, Susana Ponte, Carl-Philipp J Heisenberg, Luis Almeida, André S. Nunes, Nuno A.M. Araújo, and Antonio Jacinto. “Occluding Junctions as Novel Regulators of Tissue Mechanics during Wound Repair.” Journal of Cell Biology. Rockefeller University Press, 2018. https://doi.org/10.1083/jcb.201804048.","ama":"Carvalho L, Patricio P, Ponte S, et al. Occluding junctions as novel regulators of tissue mechanics during wound repair. Journal of Cell Biology. 2018;217(12):4267-4283. doi:10.1083/jcb.201804048","apa":"Carvalho, L., Patricio, P., Ponte, S., Heisenberg, C.-P. J., Almeida, L., Nunes, A. S., … Jacinto, A. (2018). Occluding junctions as novel regulators of tissue mechanics during wound repair. Journal of Cell Biology. Rockefeller University Press. https://doi.org/10.1083/jcb.201804048","ieee":"L. Carvalho et al., “Occluding junctions as novel regulators of tissue mechanics during wound repair,” Journal of Cell Biology, vol. 217, no. 12. Rockefeller University Press, pp. 4267–4283, 2018.","ista":"Carvalho L, Patricio P, Ponte S, Heisenberg C-PJ, Almeida L, Nunes AS, Araújo NAM, Jacinto A. 2018. Occluding junctions as novel regulators of tissue mechanics during wound repair. Journal of Cell Biology. 217(12), 4267–4283."},"publication":"Journal of Cell Biology","issue":"12","abstract":[{"text":"In epithelial tissues, cells tightly connect to each other through cell–cell junctions, but they also present the remarkable capacity of reorganizing themselves without compromising tissue integrity. Upon injury, simple epithelia efficiently resolve small lesions through the action of actin cytoskeleton contractile structures at the wound edge and cellular rearrangements. However, the underlying mechanisms and how they cooperate are still poorly understood. In this study, we combine live imaging and theoretical modeling to reveal a novel and indispensable role for occluding junctions (OJs) in this process. We demonstrate that OJ loss of function leads to defects in wound-closure dynamics: instead of contracting, wounds dramatically increase their area. OJ mutants exhibit phenotypes in cell shape, cellular rearrangements, and mechanical properties as well as in actin cytoskeleton dynamics at the wound edge. We propose that OJs are essential for wound closure by impacting on epithelial mechanics at the tissue level, which in turn is crucial for correct regulation of the cellular events occurring at the wound edge.","lang":"eng"}],"type":"journal_article","oa_version":"Submitted Version","intvolume":" 217","title":"Occluding junctions as novel regulators of tissue mechanics during wound repair","status":"public","_id":"5676","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publication_identifier":{"issn":["00219525"]},"month":"12","language":[{"iso":"eng"}],"doi":"10.1083/jcb.201804048","project":[{"name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7","grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425"}],"isi":1,"quality_controlled":"1","oa":1,"main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pubmed/30228162","open_access":"1"}],"external_id":{"pmid":["30228162 "],"isi":["000451960800018"]},"ec_funded":1,"volume":217,"date_created":"2018-12-16T22:59:19Z","date_updated":"2023-09-13T09:11:17Z","author":[{"full_name":"Carvalho, Lara","first_name":"Lara","last_name":"Carvalho"},{"last_name":"Patricio","first_name":"Pedro","full_name":"Patricio, Pedro"},{"first_name":"Susana","last_name":"Ponte","full_name":"Ponte, Susana"},{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"},{"first_name":"Luis","last_name":"Almeida","full_name":"Almeida, Luis"},{"first_name":"André S.","last_name":"Nunes","full_name":"Nunes, André S."},{"first_name":"Nuno A.M.","last_name":"Araújo","full_name":"Araújo, Nuno A.M."},{"full_name":"Jacinto, Antonio","first_name":"Antonio","last_name":"Jacinto"}],"publisher":"Rockefeller University Press","department":[{"_id":"CaHe"}],"publication_status":"published","pmid":1,"year":"2018"},{"publication_identifier":{"eissn":["2041-2657"],"issn":["2041-2649"]},"month":"09","oa":1,"main_file_link":[{"url":"https://doi.org/10.1093/bfgp/ely007","open_access":"1"}],"external_id":{"isi":["000456054400004"],"pmid":["29579140"]},"isi":1,"quality_controlled":"1","doi":"10.1093/bfgp/ely007","language":[{"iso":"eng"}],"pmid":1,"year":"2018","acknowledgement":"This work was supported by JSPS overseas research fellowships (Y.M.) and SENSHIN Medical Research Foundation (K.K.T.).","publisher":"Oxford University Press","department":[{"_id":"CaHe"}],"publication_status":"published","author":[{"id":"4968E7C8-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2853-8051","first_name":"Moriyama","last_name":"Yuuta","full_name":"Yuuta, Moriyama"},{"first_name":"Kazuko","last_name":"Koshiba-Takeuchi","full_name":"Koshiba-Takeuchi, Kazuko"}],"volume":17,"date_updated":"2023-09-19T15:11:22Z","date_created":"2022-03-18T12:40:35Z","scopus_import":"1","keyword":["Genetics","Molecular Biology","Biochemistry","General Medicine"],"article_processing_charge":"No","day":"01","citation":{"ista":"Yuuta M, Koshiba-Takeuchi K. 2018. Significance of whole-genome duplications on the emergence of evolutionary novelties. Briefings in Functional Genomics. 17(5), 329–338.","ieee":"M. Yuuta and K. Koshiba-Takeuchi, “Significance of whole-genome duplications on the emergence of evolutionary novelties,” Briefings in Functional Genomics, vol. 17, no. 5. Oxford University Press, pp. 329–338, 2018.","apa":"Yuuta, M., & Koshiba-Takeuchi, K. (2018). Significance of whole-genome duplications on the emergence of evolutionary novelties. Briefings in Functional Genomics. Oxford University Press. https://doi.org/10.1093/bfgp/ely007","ama":"Yuuta M, Koshiba-Takeuchi K. Significance of whole-genome duplications on the emergence of evolutionary novelties. Briefings in Functional Genomics. 2018;17(5):329-338. doi:10.1093/bfgp/ely007","chicago":"Yuuta, Moriyama, and Kazuko Koshiba-Takeuchi. “Significance of Whole-Genome Duplications on the Emergence of Evolutionary Novelties.” Briefings in Functional Genomics. Oxford University Press, 2018. https://doi.org/10.1093/bfgp/ely007.","mla":"Yuuta, Moriyama, and Kazuko Koshiba-Takeuchi. “Significance of Whole-Genome Duplications on the Emergence of Evolutionary Novelties.” Briefings in Functional Genomics, vol. 17, no. 5, Oxford University Press, 2018, pp. 329–38, doi:10.1093/bfgp/ely007.","short":"M. Yuuta, K. Koshiba-Takeuchi, Briefings in Functional Genomics 17 (2018) 329–338."},"publication":"Briefings in Functional Genomics","page":"329-338","article_type":"original","date_published":"2018-09-01T00:00:00Z","type":"journal_article","issue":"5","abstract":[{"text":"Acquisition of evolutionary novelties is a fundamental process for adapting to the external environment and invading new niches and results in the diversification of life, which we can see in the world today. How such novel phenotypic traits are acquired in the course of evolution and are built up in developing embryos has been a central question in biology. Whole-genome duplication (WGD) is a process of genome doubling that supplies raw genetic materials and increases genome complexity. Recently, it has been gradually revealed that WGD and subsequent fate changes of duplicated genes can facilitate phenotypic evolution. Here, we review the current understanding of the relationship between WGD and the acquisition of evolutionary novelties. We show some examples of this link and discuss how WGD and subsequent duplicated genes can facilitate phenotypic evolution as well as when such genomic doubling can be advantageous for adaptation.","lang":"eng"}],"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","_id":"10880","intvolume":" 17","title":"Significance of whole-genome duplications on the emergence of evolutionary novelties","status":"public","oa_version":"Published Version"},{"supervisor":[{"full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J"}],"degree_awarded":"PhD","language":[{"iso":"eng"}],"doi":"10.15479/AT:ISTA:TH_1031","oa":1,"month":"06","publication_identifier":{"issn":["2663-337X"]},"date_updated":"2023-09-07T12:48:16Z","date_created":"2018-12-11T11:44:21Z","author":[{"first_name":"Daniel","last_name":"Capek","id":"31C42484-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5199-9940","full_name":"Capek, Daniel"}],"related_material":{"record":[{"id":"1100","relation":"part_of_dissertation","status":"public"},{"relation":"part_of_dissertation","status":"public","id":"661"},{"id":"676","status":"public","relation":"part_of_dissertation"}]},"publication_status":"published","publisher":"Institute of Science and Technology Austria","department":[{"_id":"CaHe"}],"year":"2018","file_date_updated":"2021-02-11T23:30:21Z","publist_id":"8004","date_published":"2018-06-22T00:00:00Z","page":"95","citation":{"ama":"Capek D. Optogenetic Frizzled 7 reveals a permissive function of Wnt/PCP signaling in directed mesenchymal cell migration. 2018. doi:10.15479/AT:ISTA:TH_1031","ieee":"D. Capek, “Optogenetic Frizzled 7 reveals a permissive function of Wnt/PCP signaling in directed mesenchymal cell migration,” Institute of Science and Technology Austria, 2018.","apa":"Capek, D. (2018). Optogenetic Frizzled 7 reveals a permissive function of Wnt/PCP signaling in directed mesenchymal cell migration. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:TH_1031","ista":"Capek D. 2018. Optogenetic Frizzled 7 reveals a permissive function of Wnt/PCP signaling in directed mesenchymal cell migration. Institute of Science and Technology Austria.","short":"D. Capek, Optogenetic Frizzled 7 Reveals a Permissive Function of Wnt/PCP Signaling in Directed Mesenchymal Cell Migration, Institute of Science and Technology Austria, 2018.","mla":"Capek, Daniel. Optogenetic Frizzled 7 Reveals a Permissive Function of Wnt/PCP Signaling in Directed Mesenchymal Cell Migration. Institute of Science and Technology Austria, 2018, doi:10.15479/AT:ISTA:TH_1031.","chicago":"Capek, Daniel. “Optogenetic Frizzled 7 Reveals a Permissive Function of Wnt/PCP Signaling in Directed Mesenchymal Cell Migration.” Institute of Science and Technology Austria, 2018. https://doi.org/10.15479/AT:ISTA:TH_1031."},"day":"22","article_processing_charge":"No","has_accepted_license":"1","file":[{"file_name":"2018_Thesis_Capek.pdf","access_level":"open_access","content_type":"application/pdf","file_size":31576521,"creator":"dernst","relation":"main_file","file_id":"6238","embargo":"2019-06-25","date_created":"2019-04-08T13:42:26Z","date_updated":"2021-02-11T11:17:17Z","checksum":"d3eca3dcacb67bffdde6e6609c31cdd0"},{"checksum":"876deb14067e638aba65d209668bd821","date_created":"2019-04-08T13:42:27Z","date_updated":"2021-02-11T23:30:21Z","relation":"source_file","file_id":"6239","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","file_size":38992956,"creator":"dernst","access_level":"closed","embargo_to":"open_access","file_name":"2018_Thesis_Capek_source.docx"}],"oa_version":"Published Version","pubrep_id":"1031","status":"public","ddc":["570","591","596"],"title":"Optogenetic Frizzled 7 reveals a permissive function of Wnt/PCP signaling in directed mesenchymal cell migration","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","_id":"50","abstract":[{"text":"The Wnt/planar cell polarity (Wnt/PCP) pathway determines planar polarity of epithelial cells in both vertebrates and invertebrates. The role that Wnt/PCP signaling plays in mesenchymal contexts, however, is only poorly understood. While previous studies have demonstrated the capacity of Wnt/PCP signaling to polarize and guide directed migration of mesenchymal cells, it remains unclear whether endogenous Wnt/PCP signaling performs these functions instructively, as it does in epithelial cells. Here we developed a light-switchable version of the Wnt/PCP receptor Frizzled 7 (Fz7) to unambiguously distinguish between an instructive and a permissive role of Wnt/PCP signaling for the directional collective migration of mesendoderm progenitor cells during zebrafish gastrulation. We show that prechordal plate (ppl) cell migration is defective in maternal-zygotic fz7a and fz7b (MZ fz7a,b) double mutant embryos, and that Fz7 functions cell-autonomously in this process by promoting ppl cell protrusion formation and directed migration. We further show that local activation of Fz7 can direct ppl cell migration both in vitro and in vivo. Surprisingly, however, uniform Fz7 activation is sufficient to fully rescue the ppl cell migration defect in MZ fz7a,b mutant embryos, indicating that Wnt/PCP signaling functions permissively rather than instructively in directed mesendoderm cell migration during zebrafish gastrulation.","lang":"eng"}],"alternative_title":["ISTA Thesis"],"type":"dissertation"},{"title":"Multiscale force sensing in development","publication_status":"published","status":"public","publisher":"Nature Publishing Group","intvolume":" 19","department":[{"_id":"CaHe"}],"year":"2017","_id":"678","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","date_updated":"2021-01-12T08:08:59Z","date_created":"2018-12-11T11:47:53Z","oa_version":"None","volume":19,"author":[{"full_name":"Petridou, Nicoletta","orcid":"0000-0002-8451-1195","id":"2A003F6C-F248-11E8-B48F-1D18A9856A87","last_name":"Petridou","first_name":"Nicoletta"},{"full_name":"Spiro, Zoltan P","id":"426AD026-F248-11E8-B48F-1D18A9856A87","first_name":"Zoltan P","last_name":"Spiro"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg"}],"type":"journal_article","abstract":[{"lang":"eng","text":"The seminal observation that mechanical signals can elicit changes in biochemical signalling within cells, a process commonly termed mechanosensation and mechanotransduction, has revolutionized our understanding of the role of cell mechanics in various fundamental biological processes, such as cell motility, adhesion, proliferation and differentiation. In this Review, we will discuss how the interplay and feedback between mechanical and biochemical signals control tissue morphogenesis and cell fate specification in embryonic development."}],"issue":"6","publist_id":"7040","quality_controlled":"1","page":"581 - 588","project":[{"grant_number":"ALTF534-2016","_id":"25236028-B435-11E9-9278-68D0E5697425","name":"The generation and function of anisotropic tissue tension in zebrafish epiboly (EMBO Fellowship)"}],"publication":"Nature Cell Biology","citation":{"chicago":"Petridou, Nicoletta, Zoltan P Spiro, and Carl-Philipp J Heisenberg. “Multiscale Force Sensing in Development.” Nature Cell Biology. Nature Publishing Group, 2017. https://doi.org/10.1038/ncb3524.","short":"N. Petridou, Z.P. Spiro, C.-P.J. Heisenberg, Nature Cell Biology 19 (2017) 581–588.","mla":"Petridou, Nicoletta, et al. “Multiscale Force Sensing in Development.” Nature Cell Biology, vol. 19, no. 6, Nature Publishing Group, 2017, pp. 581–88, doi:10.1038/ncb3524.","ieee":"N. Petridou, Z. P. Spiro, and C.-P. J. Heisenberg, “Multiscale force sensing in development,” Nature Cell Biology, vol. 19, no. 6. Nature Publishing Group, pp. 581–588, 2017.","apa":"Petridou, N., Spiro, Z. P., & Heisenberg, C.-P. J. (2017). Multiscale force sensing in development. Nature Cell Biology. Nature Publishing Group. https://doi.org/10.1038/ncb3524","ista":"Petridou N, Spiro ZP, Heisenberg C-PJ. 2017. Multiscale force sensing in development. Nature Cell Biology. 19(6), 581–588.","ama":"Petridou N, Spiro ZP, Heisenberg C-PJ. Multiscale force sensing in development. Nature Cell Biology. 2017;19(6):581-588. doi:10.1038/ncb3524"},"language":[{"iso":"eng"}],"doi":"10.1038/ncb3524","date_published":"2017-05-31T00:00:00Z","scopus_import":1,"month":"05","day":"31","publication_identifier":{"issn":["14657392"]}},{"oa_version":"None","volume":145,"date_updated":"2021-01-12T08:09:23Z","date_created":"2018-12-11T11:47:55Z","author":[{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"intvolume":" 145","publisher":"Elsevier","department":[{"_id":"CaHe"}],"publication_status":"published","status":"public","title":"D'Arcy Thompson's ‘on growth and form’: From soap bubbles to tissue self organization","_id":"686","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2017","publist_id":"7024","abstract":[{"text":"Tissues are thought to behave like fluids with a given surface tension. Differences in tissue surface tension (TST) have been proposed to trigger cell sorting and tissue envelopment. D'Arcy Thompson in his seminal book ‘On Growth and Form’ has introduced this concept of differential TST as a key physical mechanism dictating tissue formation and organization within the developing organism. Over the past century, many studies have picked up the concept of differential TST and analyzed the role and cell biological basis of TST in development, underlining the importance and influence of this concept in developmental biology.","lang":"eng"}],"type":"journal_article","language":[{"iso":"eng"}],"date_published":"2017-06-01T00:00:00Z","doi":"10.1016/j.mod.2017.03.006","page":"32 - 37","quality_controlled":"1","citation":{"ista":"Heisenberg C-PJ. 2017. D’Arcy Thompson’s ‘on growth and form’: From soap bubbles to tissue self organization. Mechanisms of Development. 145, 32–37.","ieee":"C.-P. J. Heisenberg, “D’Arcy Thompson’s ‘on growth and form’: From soap bubbles to tissue self organization,” Mechanisms of Development, vol. 145. Elsevier, pp. 32–37, 2017.","apa":"Heisenberg, C.-P. J. (2017). D’Arcy Thompson’s ‘on growth and form’: From soap bubbles to tissue self organization. Mechanisms of Development. Elsevier. https://doi.org/10.1016/j.mod.2017.03.006","ama":"Heisenberg C-PJ. D’Arcy Thompson’s ‘on growth and form’: From soap bubbles to tissue self organization. Mechanisms of Development. 2017;145:32-37. doi:10.1016/j.mod.2017.03.006","chicago":"Heisenberg, Carl-Philipp J. “D’Arcy Thompson’s ‘on Growth and Form’: From Soap Bubbles to Tissue Self Organization.” Mechanisms of Development. Elsevier, 2017. https://doi.org/10.1016/j.mod.2017.03.006.","mla":"Heisenberg, Carl-Philipp J. “D’Arcy Thompson’s ‘on Growth and Form’: From Soap Bubbles to Tissue Self Organization.” Mechanisms of Development, vol. 145, Elsevier, 2017, pp. 32–37, doi:10.1016/j.mod.2017.03.006.","short":"C.-P.J. Heisenberg, Mechanisms of Development 145 (2017) 32–37."},"publication":"Mechanisms of Development","publication_identifier":{"issn":["09254773"]},"month":"06","day":"01","scopus_import":1},{"_id":"1067","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","intvolume":" 40","ddc":["572","597"],"title":"The physical basis of coordinated tissue spreading in zebrafish gastrulation","status":"public","pubrep_id":"869","file":[{"file_id":"4849","relation":"main_file","date_updated":"2018-12-12T10:10:57Z","date_created":"2018-12-12T10:10:57Z","file_name":"IST-2017-869-v1+1_1-s2.0-S1534580717300370-main.pdf","access_level":"open_access","creator":"system","content_type":"application/pdf","file_size":6866187}],"oa_version":"Published Version","type":"journal_article","issue":"4","abstract":[{"lang":"eng","text":"Embryo morphogenesis relies on highly coordinated movements of different tissues. However, remarkably little is known about how tissues coordinate their movements to shape the embryo. In zebrafish embryogenesis, coordinated tissue movements first become apparent during “doming,” when the blastoderm begins to spread over the yolk sac, a process involving coordinated epithelial surface cell layer expansion and mesenchymal deep cell intercalations. Here, we find that active surface cell expansion represents the key process coordinating tissue movements during doming. By using a combination of theory and experiments, we show that epithelial surface cells not only trigger blastoderm expansion by reducing tissue surface tension, but also drive blastoderm thinning by inducing tissue contraction through radial deep cell intercalations. Thus, coordinated tissue expansion and thinning during doming relies on surface cells simultaneously controlling tissue surface tension and radial tissue contraction."}],"citation":{"ama":"Morita H, Grigolon S, Bock M, Krens G, Salbreux G, Heisenberg C-PJ. The physical basis of coordinated tissue spreading in zebrafish gastrulation. Developmental Cell. 2017;40(4):354-366. doi:10.1016/j.devcel.2017.01.010","ieee":"H. Morita, S. Grigolon, M. Bock, G. Krens, G. Salbreux, and C.-P. J. Heisenberg, “The physical basis of coordinated tissue spreading in zebrafish gastrulation,” Developmental Cell, vol. 40, no. 4. Cell Press, pp. 354–366, 2017.","apa":"Morita, H., Grigolon, S., Bock, M., Krens, G., Salbreux, G., & Heisenberg, C.-P. J. (2017). The physical basis of coordinated tissue spreading in zebrafish gastrulation. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2017.01.010","ista":"Morita H, Grigolon S, Bock M, Krens G, Salbreux G, Heisenberg C-PJ. 2017. The physical basis of coordinated tissue spreading in zebrafish gastrulation. Developmental Cell. 40(4), 354–366.","short":"H. Morita, S. Grigolon, M. Bock, G. Krens, G. Salbreux, C.-P.J. Heisenberg, Developmental Cell 40 (2017) 354–366.","mla":"Morita, Hitoshi, et al. “The Physical Basis of Coordinated Tissue Spreading in Zebrafish Gastrulation.” Developmental Cell, vol. 40, no. 4, Cell Press, 2017, pp. 354–66, doi:10.1016/j.devcel.2017.01.010.","chicago":"Morita, Hitoshi, Silvia Grigolon, Martin Bock, Gabriel Krens, Guillaume Salbreux, and Carl-Philipp J Heisenberg. “The Physical Basis of Coordinated Tissue Spreading in Zebrafish Gastrulation.” Developmental Cell. Cell Press, 2017. https://doi.org/10.1016/j.devcel.2017.01.010."},"publication":"Developmental Cell","page":"354 - 366","date_published":"2017-02-27T00:00:00Z","scopus_import":"1","article_processing_charge":"No","has_accepted_license":"1","day":"27","year":"2017","department":[{"_id":"CaHe"}],"publisher":"Cell Press","publication_status":"published","author":[{"full_name":"Morita, Hitoshi","first_name":"Hitoshi","last_name":"Morita","id":"4C6E54C6-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Grigolon, Silvia","last_name":"Grigolon","first_name":"Silvia"},{"full_name":"Bock, Martin","first_name":"Martin","last_name":"Bock"},{"full_name":"Krens, Gabriel","orcid":"0000-0003-4761-5996","id":"2B819732-F248-11E8-B48F-1D18A9856A87","last_name":"Krens","first_name":"Gabriel"},{"full_name":"Salbreux, Guillaume","last_name":"Salbreux","first_name":"Guillaume"},{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"}],"volume":40,"date_updated":"2023-09-20T12:06:27Z","date_created":"2018-12-11T11:49:58Z","publist_id":"6320","ec_funded":1,"file_date_updated":"2018-12-12T10:10:57Z","external_id":{"isi":["000395368300007"]},"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"project":[{"call_identifier":"FP7","name":"Developing High-Throughput Bioassays for Human Cancers in Zebrafish","_id":"2524F500-B435-11E9-9278-68D0E5697425","grant_number":"201439"}],"quality_controlled":"1","isi":1,"doi":"10.1016/j.devcel.2017.01.010","language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"PreCl"}],"publication_identifier":{"issn":["15345807"]},"month":"02"},{"type":"journal_article","abstract":[{"text":"Many organ surfaces are covered by a protective epithelial-cell layer. It emerges that such layers are maintained by cell stretching that triggers cell division mediated by the force-sensitive ion-channel protein Piezo1. See Letter p.118","lang":"eng"}],"issue":"7643","status":"public","title":"Cell biology: Stretched divisions","intvolume":" 543","_id":"1025","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","oa_version":"None","scopus_import":"1","day":"02","article_processing_charge":"No","page":"43 - 44","publication":"Nature","citation":{"short":"C.-P.J. Heisenberg, Nature 543 (2017) 43–44.","mla":"Heisenberg, Carl-Philipp J. “Cell Biology: Stretched Divisions.” Nature, vol. 543, no. 7643, Nature Publishing Group, 2017, pp. 43–44, doi:10.1038/nature21502.","chicago":"Heisenberg, Carl-Philipp J. “Cell Biology: Stretched Divisions.” Nature. Nature Publishing Group, 2017. https://doi.org/10.1038/nature21502.","ama":"Heisenberg C-PJ. Cell biology: Stretched divisions. Nature. 2017;543(7643):43-44. doi:10.1038/nature21502","apa":"Heisenberg, C.-P. J. (2017). Cell biology: Stretched divisions. Nature. Nature Publishing Group. https://doi.org/10.1038/nature21502","ieee":"C.-P. J. Heisenberg, “Cell biology: Stretched divisions,” Nature, vol. 543, no. 7643. Nature Publishing Group, pp. 43–44, 2017.","ista":"Heisenberg C-PJ. 2017. Cell biology: Stretched divisions. Nature. 543(7643), 43–44."},"date_published":"2017-03-02T00:00:00Z","publist_id":"6367","publication_status":"published","department":[{"_id":"CaHe"}],"publisher":"Nature Publishing Group","year":"2017","date_created":"2018-12-11T11:49:45Z","date_updated":"2023-09-22T09:26:59Z","volume":543,"author":[{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"month":"03","publication_identifier":{"issn":["00280836"]},"isi":1,"quality_controlled":"1","external_id":{"isi":["000395671500025"]},"language":[{"iso":"eng"}],"doi":"10.1038/nature21502"},{"year":"2017","department":[{"_id":"CaHe"}],"publisher":"Cell Press","publication_status":"published","author":[{"full_name":"Samwer, Matthias","last_name":"Samwer","first_name":"Matthias"},{"last_name":"Schneider","first_name":"Maximilian","full_name":"Schneider, Maximilian"},{"full_name":"Hoefler, Rudolf","last_name":"Hoefler","first_name":"Rudolf"},{"full_name":"Schmalhorst, Philipp S","last_name":"Schmalhorst","first_name":"Philipp S","orcid":"0000-0002-5795-0133","id":"309D50DA-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Jude, Julian","last_name":"Jude","first_name":"Julian"},{"full_name":"Zuber, Johannes","first_name":"Johannes","last_name":"Zuber"},{"last_name":"Gerlic","first_name":"Daniel","full_name":"Gerlic, Daniel"}],"volume":170,"date_created":"2018-12-11T11:48:35Z","date_updated":"2023-09-27T10:59:14Z","publist_id":"6848","file_date_updated":"2020-07-14T12:48:08Z","license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"oa":1,"external_id":{"isi":["000408372400014"]},"quality_controlled":"1","isi":1,"doi":"10.1016/j.cell.2017.07.038","language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"Bio"}],"publication_identifier":{"issn":["00928674"]},"month":"08","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","_id":"803","intvolume":" 170","title":"DNA cross-bridging shapes a single nucleus from a set of mitotic chromosomes","ddc":["570"],"status":"public","file":[{"date_updated":"2020-07-14T12:48:08Z","date_created":"2019-01-18T13:45:40Z","checksum":"64897b0c5373f22273f598e4672c60ff","file_id":"5852","relation":"main_file","creator":"dernst","content_type":"application/pdf","file_size":17666637,"file_name":"2017_Cell_Samwer.pdf","access_level":"open_access"}],"oa_version":"Published Version","type":"journal_article","issue":"5","abstract":[{"text":"Eukaryotic cells store their chromosomes in a single nucleus. This is important to maintain genomic integrity, as chromosomes packaged into separate nuclei (micronuclei) are prone to massive DNA damage. During mitosis, higher eukaryotes disassemble their nucleus and release individualized chromosomes for segregation. How numerous chromosomes subsequently reform a single nucleus has remained unclear. Using image-based screening of human cells, we identified barrier-to-autointegration factor (BAF) as a key factor guiding membranes to form a single nucleus. Unexpectedly, nuclear assembly does not require BAF?s association with inner nuclear membrane proteins but instead relies on BAF?s ability to bridge distant DNA sites. Live-cell imaging and in vitro reconstitution showed that BAF enriches around the mitotic chromosome ensemble to induce a densely cross-bridged chromatin layer that is mechanically stiff and limits membranes to the surface. Our study reveals that BAF-mediated changes in chromosome mechanics underlie nuclear assembly with broad implications for proper genome function.","lang":"eng"}],"citation":{"ista":"Samwer M, Schneider M, Hoefler R, Schmalhorst PS, Jude J, Zuber J, Gerlic D. 2017. DNA cross-bridging shapes a single nucleus from a set of mitotic chromosomes. Cell. 170(5), 956–972.","apa":"Samwer, M., Schneider, M., Hoefler, R., Schmalhorst, P. S., Jude, J., Zuber, J., & Gerlic, D. (2017). DNA cross-bridging shapes a single nucleus from a set of mitotic chromosomes. Cell. Cell Press. https://doi.org/10.1016/j.cell.2017.07.038","ieee":"M. Samwer et al., “DNA cross-bridging shapes a single nucleus from a set of mitotic chromosomes,” Cell, vol. 170, no. 5. Cell Press, pp. 956–972, 2017.","ama":"Samwer M, Schneider M, Hoefler R, et al. DNA cross-bridging shapes a single nucleus from a set of mitotic chromosomes. Cell. 2017;170(5):956-972. doi:10.1016/j.cell.2017.07.038","chicago":"Samwer, Matthias, Maximilian Schneider, Rudolf Hoefler, Philipp S Schmalhorst, Julian Jude, Johannes Zuber, and Daniel Gerlic. “DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes.” Cell. Cell Press, 2017. https://doi.org/10.1016/j.cell.2017.07.038.","mla":"Samwer, Matthias, et al. “DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes.” Cell, vol. 170, no. 5, Cell Press, 2017, pp. 956–72, doi:10.1016/j.cell.2017.07.038.","short":"M. Samwer, M. Schneider, R. Hoefler, P.S. Schmalhorst, J. Jude, J. Zuber, D. Gerlic, Cell 170 (2017) 956–972."},"publication":"Cell","page":"956 - 972","date_published":"2017-08-24T00:00:00Z","scopus_import":"1","article_processing_charge":"No","has_accepted_license":"1","day":"24"},{"publist_id":"6847","author":[{"first_name":"Philipp S","last_name":"Schmalhorst","id":"309D50DA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5795-0133","full_name":"Schmalhorst, Philipp S"},{"full_name":"Deluweit, Felix","last_name":"Deluweit","first_name":"Felix"},{"first_name":"Roger","last_name":"Scherrers","full_name":"Scherrers, Roger"},{"first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J"},{"full_name":"Sikora, Mateusz K","id":"2F74BCDE-F248-11E8-B48F-1D18A9856A87","last_name":"Sikora","first_name":"Mateusz K"}],"volume":13,"date_created":"2018-12-11T11:48:35Z","date_updated":"2023-09-27T10:58:45Z","acknowledgement":"P.S.S. was supported by research fellowship 2811/1-1 from the German Research Foundation (DFG), and M.S. was supported by EMBO Long Term Fellowship ALTF 187-2013 and Grant GC65-32 from the Interdisciplinary Centre for Mathematical and Computational Modelling (ICM), University of Warsaw, Poland. The authors thank Antje Potthast, Marek Cieplak, Tomasz Włodarski, and Damien Thompson for fruitful discussions and the IST Austria Scientific Computing Facility for support.","year":"2017","department":[{"_id":"CaHe"}],"publisher":"American Chemical Society","publication_status":"published","publication_identifier":{"issn":["15499618"]},"month":"10","doi":"10.1021/acs.jctc.7b00374","language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"ScienComp"}],"main_file_link":[{"open_access":"1","url":"https://arxiv.org/abs/1704.03773"}],"external_id":{"isi":["000412965700036"]},"oa":1,"quality_controlled":"1","isi":1,"issue":"10","abstract":[{"lang":"eng","text":"Polysaccharides (carbohydrates) are key regulators of a large number of cell biological processes. However, precise biochemical or genetic manipulation of these often complex structures is laborious and hampers experimental structure–function studies. Molecular Dynamics (MD) simulations provide a valuable alternative tool to generate and test hypotheses on saccharide function. Yet, currently used MD force fields often overestimate the aggregation propensity of polysaccharides, affecting the usability of those simulations. Here we tested MARTINI, a popular coarse-grained (CG) force field for biological macromolecules, for its ability to accurately represent molecular forces between saccharides. To this end, we calculated a thermodynamic solution property, the second virial coefficient of the osmotic pressure (B22). Comparison with light scattering experiments revealed a nonphysical aggregation of a prototypical polysaccharide in MARTINI, pointing at an imbalance of the nonbonded solute–solute, solute–water, and water–water interactions. This finding also applies to smaller oligosaccharides which were all found to aggregate in simulations even at moderate concentrations, well below their solubility limit. Finally, we explored the influence of the Lennard-Jones (LJ) interaction between saccharide molecules and propose a simple scaling of the LJ interaction strength that makes MARTINI more reliable for the simulation of saccharides."}],"type":"journal_article","oa_version":"Submitted Version","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","_id":"804","intvolume":" 13","title":"Overcoming the limitations of the MARTINI force field in simulations of polysaccharides","status":"public","article_processing_charge":"No","day":"10","scopus_import":"1","date_published":"2017-10-10T00:00:00Z","citation":{"chicago":"Schmalhorst, Philipp S, Felix Deluweit, Roger Scherrers, Carl-Philipp J Heisenberg, and Mateusz K Sikora. “Overcoming the Limitations of the MARTINI Force Field in Simulations of Polysaccharides.” Journal of Chemical Theory and Computation. American Chemical Society, 2017. https://doi.org/10.1021/acs.jctc.7b00374.","short":"P.S. Schmalhorst, F. Deluweit, R. Scherrers, C.-P.J. Heisenberg, M.K. Sikora, Journal of Chemical Theory and Computation 13 (2017) 5039–5053.","mla":"Schmalhorst, Philipp S., et al. “Overcoming the Limitations of the MARTINI Force Field in Simulations of Polysaccharides.” Journal of Chemical Theory and Computation, vol. 13, no. 10, American Chemical Society, 2017, pp. 5039–53, doi:10.1021/acs.jctc.7b00374.","ieee":"P. S. Schmalhorst, F. Deluweit, R. Scherrers, C.-P. J. Heisenberg, and M. K. Sikora, “Overcoming the limitations of the MARTINI force field in simulations of polysaccharides,” Journal of Chemical Theory and Computation, vol. 13, no. 10. American Chemical Society, pp. 5039–5053, 2017.","apa":"Schmalhorst, P. S., Deluweit, F., Scherrers, R., Heisenberg, C.-P. J., & Sikora, M. K. (2017). Overcoming the limitations of the MARTINI force field in simulations of polysaccharides. Journal of Chemical Theory and Computation. American Chemical Society. https://doi.org/10.1021/acs.jctc.7b00374","ista":"Schmalhorst PS, Deluweit F, Scherrers R, Heisenberg C-PJ, Sikora MK. 2017. Overcoming the limitations of the MARTINI force field in simulations of polysaccharides. Journal of Chemical Theory and Computation. 13(10), 5039–5053.","ama":"Schmalhorst PS, Deluweit F, Scherrers R, Heisenberg C-PJ, Sikora MK. Overcoming the limitations of the MARTINI force field in simulations of polysaccharides. Journal of Chemical Theory and Computation. 2017;13(10):5039-5053. doi:10.1021/acs.jctc.7b00374"},"publication":"Journal of Chemical Theory and Computation","page":"5039 - 5053"},{"status":"public","ddc":["570","590"],"title":"Cell adhesion and cell fate: An effective feedback loop during zebrafish gastrulation","_id":"961","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","file":[{"date_created":"2019-04-05T08:36:52Z","date_updated":"2020-07-14T12:48:16Z","checksum":"242f88c87f2cf267bf05049fa26a687b","relation":"source_file","file_id":"6205","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","file_size":14497822,"creator":"dernst","file_name":"2017_Barone_thesis_final.docx","access_level":"closed"},{"relation":"main_file","file_id":"6206","date_updated":"2020-07-14T12:48:16Z","date_created":"2019-04-05T08:36:52Z","checksum":"ba5b0613ed8bade73a409acdd880fb8a","file_name":"2017_Barone_thesis_.pdf","access_level":"open_access","file_size":14995941,"content_type":"application/pdf","creator":"dernst"}],"oa_version":"Published Version","pubrep_id":"825","alternative_title":["ISTA Thesis"],"type":"dissertation","abstract":[{"text":"Cell-cell contact formation constitutes the first step in the emergence of multicellularity in evolution, thereby allowing the differentiation of specialized cell types. In metazoan development, cell-cell contact formation is thought to influence cell fate specification, and cell fate specification has been implicated in cell-cell contact formation. However, remarkably little is yet known about whether and how the interaction and feedback between cell-cell contact formation and cell fate specification affect development. Here we identify a positive feedback loop between cell-cell contact duration, morphogen signaling and mesendoderm cell fate specification during zebrafish gastrulation. We show that long lasting cell-cell contacts enhance the competence of prechordal plate (ppl) progenitor cells to respond to Nodal signaling, required for proper ppl cell fate specification. We further show that Nodal signalling romotes ppl cell-cell contact duration, thereby generating an effective positive feedback loop between ppl cell-cell contact duration and cell fate specification. Finally, by using a combination of theoretical modeling and experimentation, we show that this feedback loop determines whether anterior axial mesendoderm cells become ppl progenitors or, instead, turn into endoderm progenitors. Our findings reveal that the gene regulatory networks leading to cell fate diversification within the developing embryo are controlled by the interdependent activities of cell-cell signaling and contact formation.","lang":"eng"}],"page":"109","citation":{"chicago":"Barone, Vanessa. “Cell Adhesion and Cell Fate: An Effective Feedback Loop during Zebrafish Gastrulation.” Institute of Science and Technology Austria, 2017. https://doi.org/10.15479/AT:ISTA:th_825.","short":"V. Barone, Cell Adhesion and Cell Fate: An Effective Feedback Loop during Zebrafish Gastrulation, Institute of Science and Technology Austria, 2017.","mla":"Barone, Vanessa. Cell Adhesion and Cell Fate: An Effective Feedback Loop during Zebrafish Gastrulation. Institute of Science and Technology Austria, 2017, doi:10.15479/AT:ISTA:th_825.","apa":"Barone, V. (2017). Cell adhesion and cell fate: An effective feedback loop during zebrafish gastrulation. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:th_825","ieee":"V. Barone, “Cell adhesion and cell fate: An effective feedback loop during zebrafish gastrulation,” Institute of Science and Technology Austria, 2017.","ista":"Barone V. 2017. Cell adhesion and cell fate: An effective feedback loop during zebrafish gastrulation. Institute of Science and Technology Austria.","ama":"Barone V. Cell adhesion and cell fate: An effective feedback loop during zebrafish gastrulation. 2017. doi:10.15479/AT:ISTA:th_825"},"date_published":"2017-03-01T00:00:00Z","has_accepted_license":"1","article_processing_charge":"No","day":"01","department":[{"_id":"CaHe"}],"publisher":"Institute of Science and Technology Austria","publication_status":"published","acknowledgement":"Many people accompanied me during this trip: I would not have reached my destination nor \r\nenjoyed the travelling without them. First of all, thanks to CP. Thanks for making me part of \r\nyour team, always full of diverse, interesting and incredibly competent people and thanks for \r\nall the good science I witnessed and participated in. It has been a \r\nblast, an incredibly \r\nexciting one! Thanks to JLo, for teaching me how to master my pipettes and showing me \r\nthat science is a lot of fun. Many, many thanks to Gabby for teaching me basically everything \r\nabout zebrafish and being always there to advice, sugge\r\nst, support...and play fussball! \r\nThank you to Julien, for the critical eye on things, Pedro, for all the invaluable feedback and \r\nthe amazing kicker matches, and Keisuke, for showing me the light, and to the three of them \r\ntogether for all the good laughs we\r\nhad. My start in Vienna would have been a lot more \r\ndifficult without you guys. Also it would not have been possible without Elena and Inês: \r\nthanks for helping setting up this lab and for the dinners in Gugging. Thanks to Martin, for \r\nhelping me understand \r\nthe physics behind biology. Thanks to Philipp, for the interest and \r\nadvice, and to Michael, for the Viennise take on things. Thanks to Julia, for putting up with \r\nbeing our technician and becoming a friend in the process. And now to the newest members \r\nof th\r\ne lab. Thanks to Daniel for the enthusiasm and the neverending energy and for all your \r\nhelp over the years: thank you! To Jana, for showing me that one doesn’t give up, no matter \r\nwhat. To Shayan, for being such a motivated student. To Matt, for helping out\r\nwith coding \r\nand for finding punk solutions to data analysis problems. Thanks to all the members of the \r\nlab, Verena, Hitoshi, Silvia, Conny, Karla, Nicoletta, Zoltan, Peng, Benoit, Roland, Yuuta and \r\nFeyza, for the wonderful atmosphere in the lab. Many than\r\nks to Koni and Deborah: doing \r\nexperiments would have been much more difficult without your help. Special thanks to Katjia \r\nfor setting up an amazing imaging facility and for building the best team, Robert, Nasser, \r\nAnna and Doreen: thank you for putting up w\r\nith all the late sortings and for helping with all \r\nthe technical problems. Thanks to Eva, Verena and Matthias for keeping the fish happy. Big \r\nthanks to Harald Janovjak for being a present and helpful committee member over the years \r\nand to Patrick Lemaire f\r\nor the helpful insight and extremely interesting discussion we had \r\nabout the project. Also, this journey would not have been the same without all the friends \r\nthat I met in Dresden and then in Vienna: Daniele, Claire, Kuba, Steffi, Harold, Dejan, Irene, \r\nFab\r\nienne, Hande, Tiago, Marianne, Jon, Srdjan, Branca, Uli, Murat, Alex, Conny, Christoph, \r\nCaro, Simone, Barbara, Felipe, Dama, Jose, Hubert and many others that filled my days with \r\nfun and support. A special thank to my family, always close even if they are \r\nkilometers away. \r\nGrazie ai miei fratelli, Nunzio e William, e alla mia mamma, per essermi sempre vicini pur \r\nvivendo a chilometri di distanza. And, last but not least, thanks to Moritz, for putting up with \r\nthe crazy life of a scientist, the living apart for\r\nso long, never knowing when things are going \r\nto happen. Thanks for being a great partner and my number one fan!","year":"2017","date_created":"2018-12-11T11:49:25Z","date_updated":"2023-09-27T14:16:45Z","related_material":{"record":[{"id":"1100","relation":"part_of_dissertation","status":"public"},{"relation":"part_of_dissertation","status":"public","id":"1537"},{"status":"public","relation":"part_of_dissertation","id":"1912"},{"id":"2926","status":"public","relation":"part_of_dissertation"},{"id":"3246","relation":"part_of_dissertation","status":"public"},{"status":"public","relation":"part_of_dissertation","id":"676"},{"id":"735","relation":"part_of_dissertation","status":"public"}]},"author":[{"full_name":"Barone, Vanessa","orcid":"0000-0003-2676-3367","id":"419EECCC-F248-11E8-B48F-1D18A9856A87","last_name":"Barone","first_name":"Vanessa"}],"publist_id":"6444","file_date_updated":"2020-07-14T12:48:16Z","oa":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"language":[{"iso":"eng"}],"supervisor":[{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"}],"degree_awarded":"PhD","doi":"10.15479/AT:ISTA:th_825","publication_identifier":{"issn":["2663-337X"]},"month":"03"},{"doi":"10.1016/j.cub.2017.07.010","language":[{"iso":"eng"}],"external_id":{"isi":["000411581800019"]},"quality_controlled":"1","isi":1,"publication_identifier":{"issn":["09609822"]},"month":"09","author":[{"full_name":"Chan, Chii","first_name":"Chii","last_name":"Chan"},{"full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"},{"full_name":"Hiiragi, Takashi","last_name":"Hiiragi","first_name":"Takashi"}],"volume":27,"date_created":"2018-12-11T11:48:11Z","date_updated":"2023-09-28T11:33:21Z","year":"2017","publisher":"Cell Press","department":[{"_id":"CaHe"}],"publication_status":"published","publist_id":"6949","date_published":"2017-09-18T00:00:00Z","citation":{"ista":"Chan C, Heisenberg C-PJ, Hiiragi T. 2017. Coordination of morphogenesis and cell fate specification in development. Current Biology. 27(18), R1024–R1035.","ieee":"C. Chan, C.-P. J. Heisenberg, and T. Hiiragi, “Coordination of morphogenesis and cell fate specification in development,” Current Biology, vol. 27, no. 18. Cell Press, pp. R1024–R1035, 2017.","apa":"Chan, C., Heisenberg, C.-P. J., & Hiiragi, T. (2017). Coordination of morphogenesis and cell fate specification in development. Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2017.07.010","ama":"Chan C, Heisenberg C-PJ, Hiiragi T. Coordination of morphogenesis and cell fate specification in development. Current Biology. 2017;27(18):R1024-R1035. doi:10.1016/j.cub.2017.07.010","chicago":"Chan, Chii, Carl-Philipp J Heisenberg, and Takashi Hiiragi. “Coordination of Morphogenesis and Cell Fate Specification in Development.” Current Biology. Cell Press, 2017. https://doi.org/10.1016/j.cub.2017.07.010.","mla":"Chan, Chii, et al. “Coordination of Morphogenesis and Cell Fate Specification in Development.” Current Biology, vol. 27, no. 18, Cell Press, 2017, pp. R1024–35, doi:10.1016/j.cub.2017.07.010.","short":"C. Chan, C.-P.J. Heisenberg, T. Hiiragi, Current Biology 27 (2017) R1024–R1035."},"publication":"Current Biology","page":"R1024 - R1035","article_processing_charge":"No","day":"18","scopus_import":"1","oa_version":"None","_id":"728","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","intvolume":" 27","status":"public","title":"Coordination of morphogenesis and cell fate specification in development","issue":"18","abstract":[{"lang":"eng","text":"During animal development, cell-fate-specific changes in gene expression can modify the material properties of a tissue and drive tissue morphogenesis. While mechanistic insights into the genetic control of tissue-shaping events are beginning to emerge, how tissue morphogenesis and mechanics can reciprocally impact cell-fate specification remains relatively unexplored. Here we review recent findings reporting how multicellular morphogenetic events and their underlying mechanical forces can feed back into gene regulatory pathways to specify cell fate. We further discuss emerging techniques that allow for the direct measurement and manipulation of mechanical signals in vivo, offering unprecedented access to study mechanotransduction during development. Examination of the mechanical control of cell fate during tissue morphogenesis will pave the way to an integrated understanding of the design principles that underlie robust tissue patterning in embryonic development."}],"type":"journal_article"},{"publisher":"Cell Press","department":[{"_id":"CaHe"}],"publication_status":"published","year":"2017","volume":42,"date_updated":"2023-09-28T11:32:49Z","date_created":"2018-12-11T11:48:11Z","author":[{"full_name":"Spiro, Zoltan P","first_name":"Zoltan P","last_name":"Spiro","id":"426AD026-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J"}],"publist_id":"6948","quality_controlled":"1","isi":1,"external_id":{"isi":["000411582800003"]},"language":[{"iso":"eng"}],"doi":"10.1016/j.devcel.2017.09.008","publication_identifier":{"issn":["15345807"]},"month":"01","intvolume":" 42","title":"Regeneration tensed up polyploidy takes the lead","status":"public","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","_id":"729","oa_version":"None","type":"journal_article","issue":"6","abstract":[{"text":"The cellular mechanisms allowing tissues to efficiently regenerate are not fully understood. In this issue of Developmental Cell, Cao et al. (2017)) discover that during zebrafish heart regeneration, epicardial cells at the leading edge of regenerating tissue undergo endoreplication, possibly due to increased tissue tension, thereby boosting their regenerative capacity.","lang":"eng"}],"page":"559 - 560","citation":{"mla":"Spiro, Zoltan P., and Carl-Philipp J. Heisenberg. “Regeneration Tensed up Polyploidy Takes the Lead.” Developmental Cell, vol. 42, no. 6, Cell Press, 2017, pp. 559–60, doi:10.1016/j.devcel.2017.09.008.","short":"Z.P. Spiro, C.-P.J. Heisenberg, Developmental Cell 42 (2017) 559–560.","chicago":"Spiro, Zoltan P, and Carl-Philipp J Heisenberg. “Regeneration Tensed up Polyploidy Takes the Lead.” Developmental Cell. Cell Press, 2017. https://doi.org/10.1016/j.devcel.2017.09.008.","ama":"Spiro ZP, Heisenberg C-PJ. Regeneration tensed up polyploidy takes the lead. Developmental Cell. 2017;42(6):559-560. doi:10.1016/j.devcel.2017.09.008","ista":"Spiro ZP, Heisenberg C-PJ. 2017. Regeneration tensed up polyploidy takes the lead. Developmental Cell. 42(6), 559–560.","apa":"Spiro, Z. P., & Heisenberg, C.-P. J. (2017). Regeneration tensed up polyploidy takes the lead. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2017.09.008","ieee":"Z. P. Spiro and C.-P. J. Heisenberg, “Regeneration tensed up polyploidy takes the lead,” Developmental Cell, vol. 42, no. 6. Cell Press, pp. 559–560, 2017."},"publication":"Developmental Cell","date_published":"2017-01-01T00:00:00Z","scopus_import":"1","article_processing_charge":"No","day":"01"},{"month":"06","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"external_id":{"isi":["000404728300001"]},"quality_controlled":"1","isi":1,"project":[{"grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","name":"International IST Postdoc Fellowship Programme"},{"call_identifier":"FWF","name":"Molecular basis of root growth inhibition by auxin","grant_number":"M02128","_id":"2572ED28-B435-11E9-9278-68D0E5697425"},{"name":"Hormone cross-talk drives nutrient dependent plant development","call_identifier":"FWF","_id":"2542D156-B435-11E9-9278-68D0E5697425","grant_number":"I 1774-B16"},{"call_identifier":"FP7","name":"Polarity and subcellular dynamics in plants","_id":"25716A02-B435-11E9-9278-68D0E5697425","grant_number":"282300"}],"doi":"10.7554/eLife.26792","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"Bio"}],"language":[{"iso":"eng"}],"article_number":"e26792","file_date_updated":"2020-07-14T12:48:15Z","ec_funded":1,"publist_id":"6471","acknowledgement":"Funding: Marie Curie Actions (FP7/2007-2013 no 291734) to Daniel von Wangenheim; Austrian Science Fund (M 2128-B21) to Matyáš Fendrych; Austrian Science Fund (FWF01_I1774S) to Eva Benková; European Research Council (FP7/2007-2013 no 282300) to Jiří Friml. \r\nThe authors are grateful to the Miba Machine Shop at IST Austria for their contribution to the microscope setup and to Yvonne Kemper for reading, understanding and correcting the manuscript.\r\n#BioimagingFacility","year":"2017","publication_status":"published","department":[{"_id":"JiFr"},{"_id":"Bio"},{"_id":"CaHe"},{"_id":"EvBe"}],"publisher":"eLife Sciences Publications","author":[{"last_name":"Von Wangenheim","first_name":"Daniel","orcid":"0000-0002-6862-1247","id":"49E91952-F248-11E8-B48F-1D18A9856A87","full_name":"Von Wangenheim, Daniel"},{"last_name":"Hauschild","first_name":"Robert","orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert"},{"id":"43905548-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-9767-8699","first_name":"Matyas","last_name":"Fendrych","full_name":"Fendrych, Matyas"},{"orcid":"0000-0003-2676-3367","id":"419EECCC-F248-11E8-B48F-1D18A9856A87","last_name":"Barone","first_name":"Vanessa","full_name":"Barone, Vanessa"},{"full_name":"Benková, Eva","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková","first_name":"Eva"},{"first_name":"Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí"}],"related_material":{"record":[{"relation":"popular_science","status":"public","id":"5566"}]},"date_created":"2018-12-11T11:49:21Z","date_updated":"2024-02-21T13:49:34Z","volume":6,"scopus_import":"1","day":"19","has_accepted_license":"1","article_processing_charge":"Yes","publication":"eLife","citation":{"short":"D. von Wangenheim, R. Hauschild, M. Fendrych, V. Barone, E. Benková, J. Friml, ELife 6 (2017).","mla":"von Wangenheim, Daniel, et al. “Live Tracking of Moving Samples in Confocal Microscopy for Vertically Grown Roots.” ELife, vol. 6, e26792, eLife Sciences Publications, 2017, doi:10.7554/eLife.26792.","chicago":"Wangenheim, Daniel von, Robert Hauschild, Matyas Fendrych, Vanessa Barone, Eva Benková, and Jiří Friml. “Live Tracking of Moving Samples in Confocal Microscopy for Vertically Grown Roots.” ELife. eLife Sciences Publications, 2017. https://doi.org/10.7554/eLife.26792.","ama":"von Wangenheim D, Hauschild R, Fendrych M, Barone V, Benková E, Friml J. Live tracking of moving samples in confocal microscopy for vertically grown roots. eLife. 2017;6. doi:10.7554/eLife.26792","ieee":"D. von Wangenheim, R. Hauschild, M. Fendrych, V. Barone, E. Benková, and J. Friml, “Live tracking of moving samples in confocal microscopy for vertically grown roots,” eLife, vol. 6. eLife Sciences Publications, 2017.","apa":"von Wangenheim, D., Hauschild, R., Fendrych, M., Barone, V., Benková, E., & Friml, J. (2017). Live tracking of moving samples in confocal microscopy for vertically grown roots. ELife. eLife Sciences Publications. https://doi.org/10.7554/eLife.26792","ista":"von Wangenheim D, Hauschild R, Fendrych M, Barone V, Benková E, Friml J. 2017. Live tracking of moving samples in confocal microscopy for vertically grown roots. eLife. 6, e26792."},"date_published":"2017-06-19T00:00:00Z","type":"journal_article","abstract":[{"text":"Roots navigate through soil integrating environmental signals to orient their growth. The Arabidopsis root is a widely used model for developmental, physiological and cell biological studies. Live imaging greatly aids these efforts, but the horizontal sample position and continuous root tip displacement present significant difficulties. Here, we develop a confocal microscope setup for vertical sample mounting and integrated directional illumination. We present TipTracker – a custom software for automatic tracking of diverse moving objects usable on various microscope setups. Combined, this enables observation of root tips growing along the natural gravity vector over prolonged periods of time, as well as the ability to induce rapid gravity or light stimulation. We also track migrating cells in the developing zebrafish embryo, demonstrating the utility of this system in the acquisition of high-resolution data sets of dynamic samples. We provide detailed descriptions of the tools enabling the easy implementation on other microscopes.","lang":"eng"}],"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","_id":"946","ddc":["570"],"title":"Live tracking of moving samples in confocal microscopy for vertically grown roots","status":"public","intvolume":" 6","pubrep_id":"847","oa_version":"Published Version","file":[{"file_id":"5315","relation":"main_file","checksum":"9af3398cb0d81f99d79016a616df22e9","date_created":"2018-12-12T10:17:57Z","date_updated":"2020-07-14T12:48:15Z","access_level":"open_access","file_name":"IST-2017-847-v1+1_elife-26792-v2.pdf","creator":"system","content_type":"application/pdf","file_size":19581847}]},{"file":[{"file_id":"6905","relation":"main_file","date_created":"2019-09-24T06:56:22Z","date_updated":"2020-07-14T12:47:39Z","checksum":"bc25125fb664706cdf180e061429f91d","file_name":"2017_Development_Krens.pdf","access_level":"open_access","creator":"dernst","content_type":"application/pdf","file_size":8194516}],"oa_version":"Published Version","title":"Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation","status":"public","ddc":["570"],"intvolume":" 144","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"676","abstract":[{"lang":"eng","text":"The segregation of different cell types into distinct tissues is a fundamental process in metazoan development. Differences in cell adhesion and cortex tension are commonly thought to drive cell sorting by regulating tissue surface tension (TST). However, the role that differential TST plays in cell segregation within the developing embryo is as yet unclear. Here, we have analyzed the role of differential TST for germ layer progenitor cell segregation during zebrafish gastrulation. Contrary to previous observations that differential TST drives germ layer progenitor cell segregation in vitro, we show that germ layers display indistinguishable TST within the gastrulating embryo, arguing against differential TST driving germ layer progenitor cell segregation in vivo. We further show that the osmolarity of the interstitial fluid (IF) is an important factor that influences germ layer TST in vivo, and that lower osmolarity of the IF compared with standard cell culture medium can explain why germ layers display differential TST in culture but not in vivo. Finally, we show that directed migration of mesendoderm progenitors is required for germ layer progenitor cell segregation and germ layer formation."}],"issue":"10","type":"journal_article","date_published":"2017-05-15T00:00:00Z","article_type":"original","page":"1798 - 1806","publication":"Development","citation":{"ama":"Krens G, Veldhuis J, Barone V, et al. Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation. Development. 2017;144(10):1798-1806. doi:10.1242/dev.144964","ista":"Krens G, Veldhuis J, Barone V, Capek D, Maître J-L, Brodland W, Heisenberg C-PJ. 2017. Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation. Development. 144(10), 1798–1806.","ieee":"G. Krens et al., “Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation,” Development, vol. 144, no. 10. Company of Biologists, pp. 1798–1806, 2017.","apa":"Krens, G., Veldhuis, J., Barone, V., Capek, D., Maître, J.-L., Brodland, W., & Heisenberg, C.-P. J. (2017). Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation. Development. Company of Biologists. https://doi.org/10.1242/dev.144964","mla":"Krens, Gabriel, et al. “Interstitial Fluid Osmolarity Modulates the Action of Differential Tissue Surface Tension in Progenitor Cell Segregation during Gastrulation.” Development, vol. 144, no. 10, Company of Biologists, 2017, pp. 1798–806, doi:10.1242/dev.144964.","short":"G. Krens, J. Veldhuis, V. Barone, D. Capek, J.-L. Maître, W. Brodland, C.-P.J. Heisenberg, Development 144 (2017) 1798–1806.","chicago":"Krens, Gabriel, Jim Veldhuis, Vanessa Barone, Daniel Capek, Jean-Léon Maître, Wayne Brodland, and Carl-Philipp J Heisenberg. “Interstitial Fluid Osmolarity Modulates the Action of Differential Tissue Surface Tension in Progenitor Cell Segregation during Gastrulation.” Development. Company of Biologists, 2017. https://doi.org/10.1242/dev.144964."},"day":"15","article_processing_charge":"No","has_accepted_license":"1","scopus_import":1,"date_created":"2018-12-11T11:47:52Z","date_updated":"2024-03-28T23:30:26Z","volume":144,"author":[{"full_name":"Krens, Gabriel","orcid":"0000-0003-4761-5996","id":"2B819732-F248-11E8-B48F-1D18A9856A87","last_name":"Krens","first_name":"Gabriel"},{"last_name":"Veldhuis","first_name":"Jim","full_name":"Veldhuis, Jim"},{"full_name":"Barone, Vanessa","first_name":"Vanessa","last_name":"Barone","id":"419EECCC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2676-3367"},{"full_name":"Capek, Daniel","last_name":"Capek","first_name":"Daniel","orcid":"0000-0001-5199-9940","id":"31C42484-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Maître","first_name":"Jean-Léon","orcid":"0000-0002-3688-1474","id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87","full_name":"Maître, Jean-Léon"},{"first_name":"Wayne","last_name":"Brodland","full_name":"Brodland, Wayne"},{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"}],"related_material":{"record":[{"id":"961","status":"public","relation":"dissertation_contains"},{"status":"public","relation":"dissertation_contains","id":"50"}]},"publication_status":"published","department":[{"_id":"Bio"},{"_id":"CaHe"}],"publisher":"Company of Biologists","year":"2017","pmid":1,"file_date_updated":"2020-07-14T12:47:39Z","publist_id":"7047","language":[{"iso":"eng"}],"doi":"10.1242/dev.144964","quality_controlled":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"external_id":{"pmid":["28512197"]},"oa":1,"month":"05","publication_identifier":{"issn":["09501991"]}},{"publist_id":"7074","ec_funded":1,"related_material":{"record":[{"id":"50","relation":"dissertation_contains","status":"public"},{"relation":"dissertation_contains","status":"public","id":"8350"}]},"author":[{"id":"3FE6E4E8-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5920-9090","first_name":"Michael","last_name":"Smutny","full_name":"Smutny, Michael"},{"full_name":"Ákos, Zsuzsa","last_name":"Ákos","first_name":"Zsuzsa"},{"full_name":"Grigolon, Silvia","first_name":"Silvia","last_name":"Grigolon"},{"id":"40B34FE2-F248-11E8-B48F-1D18A9856A87","last_name":"Shamipour","first_name":"Shayan","full_name":"Shamipour, Shayan"},{"first_name":"Verena","last_name":"Ruprecht","full_name":"Ruprecht, Verena"},{"full_name":"Capek, Daniel","orcid":"0000-0001-5199-9940","id":"31C42484-F248-11E8-B48F-1D18A9856A87","last_name":"Capek","first_name":"Daniel"},{"id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87","last_name":"Behrndt","first_name":"Martin","full_name":"Behrndt, Martin"},{"full_name":"Papusheva, Ekaterina","id":"41DB591E-F248-11E8-B48F-1D18A9856A87","first_name":"Ekaterina","last_name":"Papusheva"},{"first_name":"Masazumi","last_name":"Tada","full_name":"Tada, Masazumi"},{"full_name":"Hof, Björn","last_name":"Hof","first_name":"Björn","orcid":"0000-0003-2057-2754","id":"3A374330-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Vicsek, Tamás","first_name":"Tamás","last_name":"Vicsek"},{"last_name":"Salbreux","first_name":"Guillaume","full_name":"Salbreux, Guillaume"},{"full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"}],"volume":19,"date_updated":"2024-03-28T23:30:39Z","date_created":"2018-12-11T11:47:46Z","pmid":1,"year":"2017","department":[{"_id":"CaHe"},{"_id":"BjHo"},{"_id":"Bio"}],"publisher":"Nature Publishing Group","publication_status":"published","publication_identifier":{"issn":["14657392"]},"month":"03","doi":"10.1038/ncb3492","language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"SSU"}],"oa":1,"external_id":{"pmid":["28346437"]},"main_file_link":[{"open_access":"1","url":"https://europepmc.org/articles/pmc5635970"}],"project":[{"call_identifier":"FP7","name":"Decoding the complexity of turbulence at its origin","grant_number":"306589","_id":"25152F3A-B435-11E9-9278-68D0E5697425"},{"_id":"252ABD0A-B435-11E9-9278-68D0E5697425","grant_number":"I 930-B20","call_identifier":"FWF","name":"Control of Epithelial Cell Layer Spreading in Zebrafish"}],"quality_controlled":"1","abstract":[{"lang":"eng","text":"During embryonic development, mechanical forces are essential for cellular rearrangements driving tissue morphogenesis. Here, we show that in the early zebrafish embryo, friction forces are generated at the interface between anterior axial mesoderm (prechordal plate, ppl) progenitors migrating towards the animal pole and neurectoderm progenitors moving in the opposite direction towards the vegetal pole of the embryo. These friction forces lead to global rearrangement of cells within the neurectoderm and determine the position of the neural anlage. Using a combination of experiments and simulations, we show that this process depends on hydrodynamic coupling between neurectoderm and ppl as a result of E-cadherin-mediated adhesion between those tissues. Our data thus establish the emergence of friction forces at the interface between moving tissues as a critical force-generating process shaping the embryo."}],"type":"journal_article","oa_version":"Submitted Version","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"661","intvolume":" 19","status":"public","title":"Friction forces position the neural anlage","day":"27","scopus_import":1,"date_published":"2017-03-27T00:00:00Z","citation":{"ama":"Smutny M, Ákos Z, Grigolon S, et al. Friction forces position the neural anlage. Nature Cell Biology. 2017;19:306-317. doi:10.1038/ncb3492","ieee":"M. Smutny et al., “Friction forces position the neural anlage,” Nature Cell Biology, vol. 19. Nature Publishing Group, pp. 306–317, 2017.","apa":"Smutny, M., Ákos, Z., Grigolon, S., Shamipour, S., Ruprecht, V., Capek, D., … Heisenberg, C.-P. J. (2017). Friction forces position the neural anlage. Nature Cell Biology. Nature Publishing Group. https://doi.org/10.1038/ncb3492","ista":"Smutny M, Ákos Z, Grigolon S, Shamipour S, Ruprecht V, Capek D, Behrndt M, Papusheva E, Tada M, Hof B, Vicsek T, Salbreux G, Heisenberg C-PJ. 2017. Friction forces position the neural anlage. Nature Cell Biology. 19, 306–317.","short":"M. Smutny, Z. Ákos, S. Grigolon, S. Shamipour, V. Ruprecht, D. Capek, M. Behrndt, E. Papusheva, M. Tada, B. Hof, T. Vicsek, G. Salbreux, C.-P.J. Heisenberg, Nature Cell Biology 19 (2017) 306–317.","mla":"Smutny, Michael, et al. “Friction Forces Position the Neural Anlage.” Nature Cell Biology, vol. 19, Nature Publishing Group, 2017, pp. 306–17, doi:10.1038/ncb3492.","chicago":"Smutny, Michael, Zsuzsa Ákos, Silvia Grigolon, Shayan Shamipour, Verena Ruprecht, Daniel Capek, Martin Behrndt, et al. “Friction Forces Position the Neural Anlage.” Nature Cell Biology. Nature Publishing Group, 2017. https://doi.org/10.1038/ncb3492."},"publication":"Nature Cell Biology","page":"306 - 317"},{"language":[{"iso":"eng"}],"doi":"10.1016/j.devcel.2017.09.014","project":[{"name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7","_id":"25681D80-B435-11E9-9278-68D0E5697425","grant_number":"291734"},{"grant_number":"I2058","_id":"252DD2A6-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Cell segregation in gastrulation: the role of cell fate specification"}],"quality_controlled":"1","isi":1,"external_id":{"isi":["000413443700011"]},"publication_identifier":{"issn":["15345807"]},"month":"10","volume":43,"date_updated":"2024-03-28T23:30:39Z","date_created":"2018-12-11T11:48:13Z","related_material":{"record":[{"id":"961","relation":"dissertation_contains","status":"public"},{"status":"public","relation":"dissertation_contains","id":"8350"}]},"author":[{"first_name":"Vanessa","last_name":"Barone","id":"419EECCC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2676-3367","full_name":"Barone, Vanessa"},{"last_name":"Lang","first_name":"Moritz","id":"29E0800A-F248-11E8-B48F-1D18A9856A87","full_name":"Lang, Moritz"},{"first_name":"Gabriel","last_name":"Krens","id":"2B819732-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4761-5996","full_name":"Krens, Gabriel"},{"last_name":"Pradhan","first_name":"Saurabh","full_name":"Pradhan, Saurabh"},{"first_name":"Shayan","last_name":"Shamipour","id":"40B34FE2-F248-11E8-B48F-1D18A9856A87","full_name":"Shamipour, Shayan"},{"full_name":"Sako, Keisuke","first_name":"Keisuke","last_name":"Sako","id":"3BED66BE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6453-8075"},{"full_name":"Sikora, Mateusz K","id":"2F74BCDE-F248-11E8-B48F-1D18A9856A87","last_name":"Sikora","first_name":"Mateusz K"},{"full_name":"Guet, Calin C","orcid":"0000-0001-6220-2052","id":"47F8433E-F248-11E8-B48F-1D18A9856A87","last_name":"Guet","first_name":"Calin C"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J"}],"department":[{"_id":"CaHe"},{"_id":"CaGu"},{"_id":"GaTk"}],"publisher":"Cell Press","publication_status":"published","year":"2017","publist_id":"6934","ec_funded":1,"date_published":"2017-10-23T00:00:00Z","page":"198 - 211","citation":{"chicago":"Barone, Vanessa, Moritz Lang, Gabriel Krens, Saurabh Pradhan, Shayan Shamipour, Keisuke Sako, Mateusz K Sikora, Calin C Guet, and Carl-Philipp J Heisenberg. “An Effective Feedback Loop between Cell-Cell Contact Duration and Morphogen Signaling Determines Cell Fate.” Developmental Cell. Cell Press, 2017. https://doi.org/10.1016/j.devcel.2017.09.014.","mla":"Barone, Vanessa, et al. “An Effective Feedback Loop between Cell-Cell Contact Duration and Morphogen Signaling Determines Cell Fate.” Developmental Cell, vol. 43, no. 2, Cell Press, 2017, pp. 198–211, doi:10.1016/j.devcel.2017.09.014.","short":"V. Barone, M. Lang, G. Krens, S. Pradhan, S. Shamipour, K. Sako, M.K. Sikora, C.C. Guet, C.-P.J. Heisenberg, Developmental Cell 43 (2017) 198–211.","ista":"Barone V, Lang M, Krens G, Pradhan S, Shamipour S, Sako K, Sikora MK, Guet CC, Heisenberg C-PJ. 2017. An effective feedback loop between cell-cell contact duration and morphogen signaling determines cell fate. Developmental Cell. 43(2), 198–211.","apa":"Barone, V., Lang, M., Krens, G., Pradhan, S., Shamipour, S., Sako, K., … Heisenberg, C.-P. J. (2017). An effective feedback loop between cell-cell contact duration and morphogen signaling determines cell fate. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2017.09.014","ieee":"V. Barone et al., “An effective feedback loop between cell-cell contact duration and morphogen signaling determines cell fate,” Developmental Cell, vol. 43, no. 2. Cell Press, pp. 198–211, 2017.","ama":"Barone V, Lang M, Krens G, et al. An effective feedback loop between cell-cell contact duration and morphogen signaling determines cell fate. Developmental Cell. 2017;43(2):198-211. doi:10.1016/j.devcel.2017.09.014"},"publication":"Developmental Cell","article_processing_charge":"No","day":"23","scopus_import":"1","oa_version":"None","intvolume":" 43","status":"public","title":"An effective feedback loop between cell-cell contact duration and morphogen signaling determines cell fate","_id":"735","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","issue":"2","abstract":[{"lang":"eng","text":"Cell-cell contact formation constitutes an essential step in evolution, leading to the differentiation of specialized cell types. However, remarkably little is known about whether and how the interplay between contact formation and fate specification affects development. Here, we identify a positive feedback loop between cell-cell contact duration, morphogen signaling, and mesendoderm cell-fate specification during zebrafish gastrulation. We show that long-lasting cell-cell contacts enhance the competence of prechordal plate (ppl) progenitor cells to respond to Nodal signaling, required for ppl cell-fate specification. We further show that Nodal signaling promotes ppl cell-cell contact duration, generating a positive feedback loop between ppl cell-cell contact duration and cell-fate specification. Finally, by combining mathematical modeling and experimentation, we show that this feedback determines whether anterior axial mesendoderm cells become ppl or, instead, turn into endoderm. Thus, the interdependent activities of cell-cell signaling and contact formation control fate diversification within the developing embryo."}],"type":"journal_article"},{"scopus_import":1,"day":"15","month":"01","quality_controlled":"1","project":[{"_id":"2529486C-B435-11E9-9278-68D0E5697425","grant_number":"T 560-B17","name":"Cell- and Tissue Mechanics in Zebrafish Germ Layer Formation","call_identifier":"FWF"}],"publication":"Physical Review Letters","citation":{"ama":"Callan Jones A, Ruprecht V, Wieser S, Heisenberg C-PJ, Voituriez R. Cortical flow-driven shapes of nonadherent cells. Physical Review Letters. 2016;116(2). doi:10.1103/PhysRevLett.116.028102","apa":"Callan Jones, A., Ruprecht, V., Wieser, S., Heisenberg, C.-P. J., & Voituriez, R. (2016). Cortical flow-driven shapes of nonadherent cells. Physical Review Letters. American Physical Society. https://doi.org/10.1103/PhysRevLett.116.028102","ieee":"A. Callan Jones, V. Ruprecht, S. Wieser, C.-P. J. Heisenberg, and R. Voituriez, “Cortical flow-driven shapes of nonadherent cells,” Physical Review Letters, vol. 116, no. 2. American Physical Society, 2016.","ista":"Callan Jones A, Ruprecht V, Wieser S, Heisenberg C-PJ, Voituriez R. 2016. Cortical flow-driven shapes of nonadherent cells. Physical Review Letters. 116(2), 028102.","short":"A. Callan Jones, V. Ruprecht, S. Wieser, C.-P.J. Heisenberg, R. Voituriez, Physical Review Letters 116 (2016).","mla":"Callan Jones, Andrew, et al. “Cortical Flow-Driven Shapes of Nonadherent Cells.” Physical Review Letters, vol. 116, no. 2, 028102, American Physical Society, 2016, doi:10.1103/PhysRevLett.116.028102.","chicago":"Callan Jones, Andrew, Verena Ruprecht, Stefan Wieser, Carl-Philipp J Heisenberg, and Raphaël Voituriez. “Cortical Flow-Driven Shapes of Nonadherent Cells.” Physical Review Letters. American Physical Society, 2016. https://doi.org/10.1103/PhysRevLett.116.028102."},"language":[{"iso":"eng"}],"doi":"10.1103/PhysRevLett.116.028102","date_published":"2016-01-15T00:00:00Z","article_number":"028102","type":"journal_article","abstract":[{"lang":"eng","text":"Nonadherent polarized cells have been observed to have a pearlike, elongated shape. Using a minimal model that describes the cell cortex as a thin layer of contractile active gel, we show that the anisotropy of active stresses, controlled by cortical viscosity and filament ordering, can account for this morphology. The predicted shapes can be determined from the flow pattern only; they prove to be independent of the mechanism at the origin of the cortical flow, and are only weakly sensitive to the cytoplasmic rheology. In the case of actin flows resulting from a contractile instability, we propose a phase diagram of three-dimensional cell shapes that encompasses nonpolarized spherical, elongated, as well as oblate shapes, all of which have been observed in experiment."}],"issue":"2","publist_id":"6095","title":"Cortical flow-driven shapes of nonadherent cells","publication_status":"published","status":"public","intvolume":" 116","department":[{"_id":"CaHe"}],"publisher":"American Physical Society","year":"2016","_id":"1239","acknowledgement":"V. R. acknowledges support by the Austrian Science Fund (FWF): (Grant No. T560-B17).","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","date_updated":"2021-01-12T06:49:19Z","date_created":"2018-12-11T11:50:53Z","oa_version":"None","volume":116,"author":[{"full_name":"Callan Jones, Andrew","last_name":"Callan Jones","first_name":"Andrew"},{"full_name":"Ruprecht, Verena","first_name":"Verena","last_name":"Ruprecht","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4088-8633"},{"full_name":"Wieser, Stefan","last_name":"Wieser","first_name":"Stefan","orcid":"0000-0002-2670-2217","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"},{"first_name":"Raphaël","last_name":"Voituriez","full_name":"Voituriez, Raphaël"}]},{"month":"03","doi":"10.1016/j.bpj.2016.02.013","language":[{"iso":"eng"}],"oa":1,"tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"project":[{"call_identifier":"FWF","name":"Control of Epithelial Cell Layer Spreading in Zebrafish","_id":"252ABD0A-B435-11E9-9278-68D0E5697425","grant_number":"I 930-B20"}],"quality_controlled":"1","publist_id":"6079","file_date_updated":"2020-07-14T12:44:41Z","author":[{"full_name":"Saha, Arnab","last_name":"Saha","first_name":"Arnab"},{"full_name":"Nishikawa, Masatoshi","last_name":"Nishikawa","first_name":"Masatoshi"},{"full_name":"Behrndt, Martin","first_name":"Martin","last_name":"Behrndt","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg"},{"full_name":"Julicher, Frank","first_name":"Frank","last_name":"Julicher"},{"full_name":"Grill, Stephan","last_name":"Grill","first_name":"Stephan"}],"volume":110,"date_updated":"2021-01-12T06:49:23Z","date_created":"2018-12-11T11:50:56Z","year":"2016","acknowledgement":"S.W.G. acknowledges support by grant no. 281903 from the European Research Council and by grant No. GR-7271/2-1 from the Deutsche Forschungsgemeinschaft. S.W.G. and C.-P.H. acknowledge support through a grant from the Fonds zur Förderung der Wissenschaftlichen Forschung and the Deutsche Forschungsgemeinschaft (No. I930-B20). We are grateful to Daniel Dickinson for providing the LP133 C. elegans strain. We thank G. Salbreux, V. K. Krishnamurthy, and J. S. Bois for fruitful discussions.","publisher":"Biophysical Society","department":[{"_id":"CaHe"}],"publication_status":"published","has_accepted_license":"1","day":"29","scopus_import":1,"date_published":"2016-03-29T00:00:00Z","citation":{"ama":"Saha A, Nishikawa M, Behrndt M, Heisenberg C-PJ, Julicher F, Grill S. Determining physical properties of the cell cortex. Biophysical Journal. 2016;110(6):1421-1429. doi:10.1016/j.bpj.2016.02.013","ista":"Saha A, Nishikawa M, Behrndt M, Heisenberg C-PJ, Julicher F, Grill S. 2016. Determining physical properties of the cell cortex. Biophysical Journal. 110(6), 1421–1429.","ieee":"A. Saha, M. Nishikawa, M. Behrndt, C.-P. J. Heisenberg, F. Julicher, and S. Grill, “Determining physical properties of the cell cortex,” Biophysical Journal, vol. 110, no. 6. Biophysical Society, pp. 1421–1429, 2016.","apa":"Saha, A., Nishikawa, M., Behrndt, M., Heisenberg, C.-P. J., Julicher, F., & Grill, S. (2016). Determining physical properties of the cell cortex. Biophysical Journal. Biophysical Society. https://doi.org/10.1016/j.bpj.2016.02.013","mla":"Saha, Arnab, et al. “Determining Physical Properties of the Cell Cortex.” Biophysical Journal, vol. 110, no. 6, Biophysical Society, 2016, pp. 1421–29, doi:10.1016/j.bpj.2016.02.013.","short":"A. Saha, M. Nishikawa, M. Behrndt, C.-P.J. Heisenberg, F. Julicher, S. Grill, Biophysical Journal 110 (2016) 1421–1429.","chicago":"Saha, Arnab, Masatoshi Nishikawa, Martin Behrndt, Carl-Philipp J Heisenberg, Frank Julicher, and Stephan Grill. “Determining Physical Properties of the Cell Cortex.” Biophysical Journal. Biophysical Society, 2016. https://doi.org/10.1016/j.bpj.2016.02.013."},"publication":"Biophysical Journal","page":"1421 - 1429","issue":"6","abstract":[{"text":"Actin and myosin assemble into a thin layer of a highly dynamic network underneath the membrane of eukaryotic cells. This network generates the forces that drive cell- and tissue-scale morphogenetic processes. The effective material properties of this active network determine large-scale deformations and other morphogenetic events. For example, the characteristic time of stress relaxation (the Maxwell time τM) in the actomyosin sets the timescale of large-scale deformation of the cortex. Similarly, the characteristic length of stress propagation (the hydrodynamic length λ) sets the length scale of slow deformations, and a large hydrodynamic length is a prerequisite for long-ranged cortical flows. Here we introduce a method to determine physical parameters of the actomyosin cortical layer in vivo directly from laser ablation experiments. For this we investigate the cortical response to laser ablation in the one-cell-stage Caenorhabditis elegans embryo and in the gastrulating zebrafish embryo. These responses can be interpreted using a coarse-grained physical description of the cortex in terms of a two-dimensional thin film of an active viscoelastic gel. To determine the Maxwell time τM, the hydrodynamic length λ, the ratio of active stress ζΔμ, and per-area friction γ, we evaluated the response to laser ablation in two different ways: by quantifying flow and density fields as a function of space and time, and by determining the time evolution of the shape of the ablated region. Importantly, both methods provide best-fit physical parameters that are in close agreement with each other and that are similar to previous estimates in the two systems. Our method provides an accurate and robust means for measuring physical parameters of the actomyosin cortical layer. It can be useful for investigations of actomyosin mechanics at the cellular-scale, but also for providing insights into the active mechanics processes that govern tissue-scale morphogenesis.","lang":"eng"}],"type":"journal_article","pubrep_id":"706","file":[{"file_id":"4845","relation":"main_file","date_created":"2018-12-12T10:10:54Z","date_updated":"2020-07-14T12:44:41Z","checksum":"c408cf2e25a25c8d711cffea524bda55","file_name":"IST-2016-706-v1+1_1-s2.0-S0006349516001582-main.pdf","access_level":"open_access","creator":"system","file_size":1965645,"content_type":"application/pdf"}],"oa_version":"Published Version","_id":"1249","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","intvolume":" 110","ddc":["572","576"],"title":"Determining physical properties of the cell cortex","status":"public"},{"language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"LifeSc"}],"doi":"10.1186/s12915-016-0294-x","project":[{"name":"Analysis of the Formation and Function of Different Cell Protusion Types During Cell Migration in Vivo","grant_number":"HE_3231/6-1","_id":"252064B8-B435-11E9-9278-68D0E5697425"}],"quality_controlled":"1","oa":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"month":"09","volume":14,"date_updated":"2021-01-12T06:49:32Z","date_created":"2018-12-11T11:51:04Z","author":[{"last_name":"Diz Muñoz","first_name":"Alba","full_name":"Diz Muñoz, Alba"},{"first_name":"Pawel","last_name":"Romanczuk","full_name":"Romanczuk, Pawel"},{"first_name":"Weimiao","last_name":"Yu","full_name":"Yu, Weimiao"},{"full_name":"Bergert, Martin","first_name":"Martin","last_name":"Bergert"},{"last_name":"Ivanovitch","first_name":"Kenzo","full_name":"Ivanovitch, Kenzo"},{"first_name":"Guillame","last_name":"Salbreux","full_name":"Salbreux, Guillame"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J"},{"last_name":"Paluch","first_name":"Ewa","full_name":"Paluch, Ewa"}],"publisher":"BioMed Central","department":[{"_id":"CaHe"}],"publication_status":"published","acknowledgement":"We thank K. Lee, C. Norden, A. Webb, and the members of the Paluch lab for\r\ncomments on the manuscript. We are grateful to P. Rørth and Peter Dieterich\r\nfor discussions, S. Ares, Y. Arboleda-Estudillo and S. Schneider for technical help,\r\nM. Biro for help with programming, and the BIOTEC/MPI-CBG and IST zebrafish\r\nand imaging facilities for help and advice at various stages of this project. This work was supported by the Max Planck Society, the Medical Research Council UK (core funding to the MRC LMCB), and by grants from the Polish Ministry of Science and Higher Education (454/N-MPG/2009/0) to EKP, the Deutsche Forschungsgemeinschaft (HE 3231/6-1 and PA 1590/1-1) to CPH and EKP, a A*Star JCO career development award (12302FG010) to WY and a Damon Runyon fellowship award to ADM (DRG 2157-12). This work was also supported by the Francis Crick Institute which receives its core funding from Cancer Research UK (FC001317), the UK Medical Research Council (FC001317), and the Wellcome Trust (FC001317) to GS.","year":"2016","publist_id":"6049","file_date_updated":"2020-07-14T12:44:42Z","article_number":"74","date_published":"2016-09-02T00:00:00Z","citation":{"ieee":"A. Diz Muñoz et al., “Steering cell migration by alternating blebs and actin-rich protrusions,” BMC Biology, vol. 14, no. 1. BioMed Central, 2016.","apa":"Diz Muñoz, A., Romanczuk, P., Yu, W., Bergert, M., Ivanovitch, K., Salbreux, G., … Paluch, E. (2016). Steering cell migration by alternating blebs and actin-rich protrusions. BMC Biology. BioMed Central. https://doi.org/10.1186/s12915-016-0294-x","ista":"Diz Muñoz A, Romanczuk P, Yu W, Bergert M, Ivanovitch K, Salbreux G, Heisenberg C-PJ, Paluch E. 2016. Steering cell migration by alternating blebs and actin-rich protrusions. BMC Biology. 14(1), 74.","ama":"Diz Muñoz A, Romanczuk P, Yu W, et al. Steering cell migration by alternating blebs and actin-rich protrusions. BMC Biology. 2016;14(1). doi:10.1186/s12915-016-0294-x","chicago":"Diz Muñoz, Alba, Pawel Romanczuk, Weimiao Yu, Martin Bergert, Kenzo Ivanovitch, Guillame Salbreux, Carl-Philipp J Heisenberg, and Ewa Paluch. “Steering Cell Migration by Alternating Blebs and Actin-Rich Protrusions.” BMC Biology. BioMed Central, 2016. https://doi.org/10.1186/s12915-016-0294-x.","short":"A. Diz Muñoz, P. Romanczuk, W. Yu, M. Bergert, K. Ivanovitch, G. Salbreux, C.-P.J. Heisenberg, E. Paluch, BMC Biology 14 (2016).","mla":"Diz Muñoz, Alba, et al. “Steering Cell Migration by Alternating Blebs and Actin-Rich Protrusions.” BMC Biology, vol. 14, no. 1, 74, BioMed Central, 2016, doi:10.1186/s12915-016-0294-x."},"publication":"BMC Biology","has_accepted_license":"1","day":"02","scopus_import":1,"oa_version":"Published Version","file":[{"access_level":"open_access","file_name":"IST-2016-695-v1+1_s12915-016-0294-x.pdf","file_size":1875695,"content_type":"application/pdf","creator":"system","relation":"main_file","file_id":"5002","checksum":"0bfa484ac69a0a560fb9a4589aeda7f6","date_updated":"2020-07-14T12:44:42Z","date_created":"2018-12-12T10:13:20Z"}],"pubrep_id":"695","intvolume":" 14","ddc":["572","576"],"status":"public","title":"Steering cell migration by alternating blebs and actin-rich protrusions","_id":"1271","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","issue":"1","abstract":[{"text":"Background: High directional persistence is often assumed to enhance the efficiency of chemotactic migration. Yet, cells in vivo usually display meandering trajectories with relatively low directional persistence, and the control and function of directional persistence during cell migration in three-dimensional environments are poorly understood. Results: Here, we use mesendoderm progenitors migrating during zebrafish gastrulation as a model system to investigate the control of directional persistence during migration in vivo. We show that progenitor cells alternate persistent run phases with tumble phases that result in cell reorientation. Runs are characterized by the formation of directed actin-rich protrusions and tumbles by enhanced blebbing. Increasing the proportion of actin-rich protrusions or blebs leads to longer or shorter run phases, respectively. Importantly, both reducing and increasing run phases result in larger spatial dispersion of the cells, indicative of reduced migration precision. A physical model quantitatively recapitulating the migratory behavior of mesendoderm progenitors indicates that the ratio of tumbling to run times, and thus the specific degree of directional persistence of migration, are critical for optimizing migration precision. Conclusions: Together, our experiments and model provide mechanistic insight into the control of migration directionality for cells moving in three-dimensional environments that combine different protrusion types, whereby the proportion of blebs to actin-rich protrusions determines the directional persistence and precision of movement by regulating the ratio of tumbling to run times.","lang":"eng"}],"type":"journal_article"},{"scopus_import":1,"day":"22","month":"09","citation":{"chicago":"Callan Jones, Andrew, Verena Ruprecht, Stefan Wieser, Carl-Philipp J Heisenberg, and Raphaël Voituriez. “Callan-Jones et Al. Reply.” Physical Review Letters. American Physical Society, 2016. https://doi.org/10.1103/PhysRevLett.117.139802.","mla":"Callan Jones, Andrew, et al. “Callan-Jones et Al. Reply.” Physical Review Letters, vol. 117, no. 13, 139802, American Physical Society, 2016, doi:10.1103/PhysRevLett.117.139802.","short":"A. Callan Jones, V. Ruprecht, S. Wieser, C.-P.J. Heisenberg, R. Voituriez, Physical Review Letters 117 (2016).","ista":"Callan Jones A, Ruprecht V, Wieser S, Heisenberg C-PJ, Voituriez R. 2016. Callan-Jones et al. Reply. Physical Review Letters. 117(13), 139802.","ieee":"A. Callan Jones, V. Ruprecht, S. Wieser, C.-P. J. Heisenberg, and R. Voituriez, “Callan-Jones et al. Reply,” Physical Review Letters, vol. 117, no. 13. American Physical Society, 2016.","apa":"Callan Jones, A., Ruprecht, V., Wieser, S., Heisenberg, C.-P. J., & Voituriez, R. (2016). Callan-Jones et al. Reply. Physical Review Letters. American Physical Society. https://doi.org/10.1103/PhysRevLett.117.139802","ama":"Callan Jones A, Ruprecht V, Wieser S, Heisenberg C-PJ, Voituriez R. Callan-Jones et al. Reply. Physical Review Letters. 2016;117(13). doi:10.1103/PhysRevLett.117.139802"},"publication":"Physical Review Letters","quality_controlled":"1","date_published":"2016-09-22T00:00:00Z","doi":"10.1103/PhysRevLett.117.139802","language":[{"iso":"eng"}],"type":"journal_article","article_number":"139802","publist_id":"6041","issue":"13","_id":"1275","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","year":"2016","intvolume":" 117","department":[{"_id":"CaHe"}],"publisher":"American Physical Society","title":"Callan-Jones et al. Reply","status":"public","publication_status":"published","author":[{"full_name":"Callan Jones, Andrew","last_name":"Callan Jones","first_name":"Andrew"},{"first_name":"Verena","last_name":"Ruprecht","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4088-8633","full_name":"Ruprecht, Verena"},{"full_name":"Wieser, Stefan","orcid":"0000-0002-2670-2217","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","last_name":"Wieser","first_name":"Stefan"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Voituriez, Raphaël","last_name":"Voituriez","first_name":"Raphaël"}],"oa_version":"None","volume":117,"date_updated":"2021-01-12T06:49:33Z","date_created":"2018-12-11T11:51:05Z"},{"issue":"6","publist_id":"6279","type":"journal_article","oa_version":"None","volume":37,"date_created":"2018-12-11T11:50:07Z","date_updated":"2023-09-07T12:56:41Z","related_material":{"record":[{"relation":"part_of_dissertation","status":"public","id":"7186"}]},"author":[{"id":"3436488C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5130-2226","first_name":"Cornelia","last_name":"Schwayer","full_name":"Schwayer, Cornelia"},{"id":"2F74BCDE-F248-11E8-B48F-1D18A9856A87","first_name":"Mateusz K","last_name":"Sikora","full_name":"Sikora, Mateusz K"},{"full_name":"Slovakova, Jana","id":"30F3F2F0-F248-11E8-B48F-1D18A9856A87","last_name":"Slovakova","first_name":"Jana"},{"full_name":"Kardos, Roland","id":"4039350E-F248-11E8-B48F-1D18A9856A87","last_name":"Kardos","first_name":"Roland"},{"full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"}],"publisher":"Cell Press","department":[{"_id":"CaHe"}],"intvolume":" 37","publication_status":"published","status":"public","title":"Actin rings of power","_id":"1096","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","year":"2016","month":"06","day":"20","scopus_import":1,"language":[{"iso":"eng"}],"date_published":"2016-06-20T00:00:00Z","doi":"10.1016/j.devcel.2016.05.024","page":"493 - 506","quality_controlled":"1","citation":{"mla":"Schwayer, Cornelia, et al. “Actin Rings of Power.” Developmental Cell, vol. 37, no. 6, Cell Press, 2016, pp. 493–506, doi:10.1016/j.devcel.2016.05.024.","short":"C. Schwayer, M.K. Sikora, J. Slovakova, R. Kardos, C.-P.J. Heisenberg, Developmental Cell 37 (2016) 493–506.","chicago":"Schwayer, Cornelia, Mateusz K Sikora, Jana Slovakova, Roland Kardos, and Carl-Philipp J Heisenberg. “Actin Rings of Power.” Developmental Cell. Cell Press, 2016. https://doi.org/10.1016/j.devcel.2016.05.024.","ama":"Schwayer C, Sikora MK, Slovakova J, Kardos R, Heisenberg C-PJ. Actin rings of power. Developmental Cell. 2016;37(6):493-506. doi:10.1016/j.devcel.2016.05.024","ista":"Schwayer C, Sikora MK, Slovakova J, Kardos R, Heisenberg C-PJ. 2016. Actin rings of power. Developmental Cell. 37(6), 493–506.","ieee":"C. Schwayer, M. K. Sikora, J. Slovakova, R. Kardos, and C.-P. J. Heisenberg, “Actin rings of power,” Developmental Cell, vol. 37, no. 6. Cell Press, pp. 493–506, 2016.","apa":"Schwayer, C., Sikora, M. K., Slovakova, J., Kardos, R., & Heisenberg, C.-P. J. (2016). Actin rings of power. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2016.05.024"},"publication":"Developmental Cell"},{"day":"19","has_accepted_license":"1","scopus_import":1,"date_published":"2016-07-19T00:00:00Z","publication":"Cell Reports","citation":{"ista":"Sako K, Pradhan S, Barone V, Inglés Prieto Á, Mueller P, Ruprecht V, Capek D, Galande S, Janovjak HL, Heisenberg C-PJ. 2016. Optogenetic control of nodal signaling reveals a temporal pattern of nodal signaling regulating cell fate specification during gastrulation. Cell Reports. 16(3), 866–877.","apa":"Sako, K., Pradhan, S., Barone, V., Inglés Prieto, Á., Mueller, P., Ruprecht, V., … Heisenberg, C.-P. J. (2016). Optogenetic control of nodal signaling reveals a temporal pattern of nodal signaling regulating cell fate specification during gastrulation. Cell Reports. Cell Press. https://doi.org/10.1016/j.celrep.2016.06.036","ieee":"K. Sako et al., “Optogenetic control of nodal signaling reveals a temporal pattern of nodal signaling regulating cell fate specification during gastrulation,” Cell Reports, vol. 16, no. 3. Cell Press, pp. 866–877, 2016.","ama":"Sako K, Pradhan S, Barone V, et al. Optogenetic control of nodal signaling reveals a temporal pattern of nodal signaling regulating cell fate specification during gastrulation. Cell Reports. 2016;16(3):866-877. doi:10.1016/j.celrep.2016.06.036","chicago":"Sako, Keisuke, Saurabh Pradhan, Vanessa Barone, Álvaro Inglés Prieto, Patrick Mueller, Verena Ruprecht, Daniel Capek, Sanjeev Galande, Harald L Janovjak, and Carl-Philipp J Heisenberg. “Optogenetic Control of Nodal Signaling Reveals a Temporal Pattern of Nodal Signaling Regulating Cell Fate Specification during Gastrulation.” Cell Reports. Cell Press, 2016. https://doi.org/10.1016/j.celrep.2016.06.036.","mla":"Sako, Keisuke, et al. “Optogenetic Control of Nodal Signaling Reveals a Temporal Pattern of Nodal Signaling Regulating Cell Fate Specification during Gastrulation.” Cell Reports, vol. 16, no. 3, Cell Press, 2016, pp. 866–77, doi:10.1016/j.celrep.2016.06.036.","short":"K. Sako, S. Pradhan, V. Barone, Á. Inglés Prieto, P. Mueller, V. Ruprecht, D. Capek, S. Galande, H.L. Janovjak, C.-P.J. Heisenberg, Cell Reports 16 (2016) 866–877."},"page":"866 - 877","abstract":[{"lang":"eng","text":"During metazoan development, the temporal pattern of morphogen signaling is critical for organizing cell fates in space and time. Yet, tools for temporally controlling morphogen signaling within the embryo are still scarce. Here, we developed a photoactivatable Nodal receptor to determine how the temporal pattern of Nodal signaling affects cell fate specification during zebrafish gastrulation. By using this receptor to manipulate the duration of Nodal signaling in vivo by light, we show that extended Nodal signaling within the organizer promotes prechordal plate specification and suppresses endoderm differentiation. Endoderm differentiation is suppressed by extended Nodal signaling inducing expression of the transcriptional repressor goosecoid (gsc) in prechordal plate progenitors, which in turn restrains Nodal signaling from upregulating the endoderm differentiation gene sox17 within these cells. Thus, optogenetic manipulation of Nodal signaling identifies a critical role of Nodal signaling duration for organizer cell fate specification during gastrulation."}],"issue":"3","type":"journal_article","pubrep_id":"754","file":[{"creator":"system","file_size":3921947,"content_type":"application/pdf","access_level":"open_access","file_name":"IST-2017-754-v1+1_1-s2.0-S2211124716307768-main.pdf","date_created":"2018-12-12T10:11:04Z","date_updated":"2018-12-12T10:11:04Z","file_id":"4857","relation":"main_file"}],"oa_version":"Published Version","_id":"1100","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","status":"public","title":"Optogenetic control of nodal signaling reveals a temporal pattern of nodal signaling regulating cell fate specification during gastrulation","ddc":["570","576"],"intvolume":" 16","month":"07","doi":"10.1016/j.celrep.2016.06.036","acknowledged_ssus":[{"_id":"SSU"}],"language":[{"iso":"eng"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"quality_controlled":"1","project":[{"_id":"2529486C-B435-11E9-9278-68D0E5697425","grant_number":"T 560-B17","call_identifier":"FWF","name":"Cell- and Tissue Mechanics in Zebrafish Germ Layer Formation"},{"grant_number":"I 812-B12","_id":"2527D5CC-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Cell Cortex and Germ Layer Formation in Zebrafish Gastrulation"},{"call_identifier":"FP7","name":"Microbial Ion Channels for Synthetic Neurobiology","_id":"25548C20-B435-11E9-9278-68D0E5697425","grant_number":"303564"}],"file_date_updated":"2018-12-12T10:11:04Z","publist_id":"6275","ec_funded":1,"author":[{"full_name":"Sako, Keisuke","first_name":"Keisuke","last_name":"Sako","id":"3BED66BE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6453-8075"},{"full_name":"Pradhan, Saurabh","first_name":"Saurabh","last_name":"Pradhan"},{"full_name":"Barone, Vanessa","first_name":"Vanessa","last_name":"Barone","id":"419EECCC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2676-3367"},{"first_name":"Álvaro","last_name":"Inglés Prieto","id":"2A9DB292-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5409-8571","full_name":"Inglés Prieto, Álvaro"},{"full_name":"Mueller, Patrick","first_name":"Patrick","last_name":"Mueller"},{"full_name":"Ruprecht, Verena","last_name":"Ruprecht","first_name":"Verena","orcid":"0000-0003-4088-8633","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87"},{"id":"31C42484-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5199-9940","first_name":"Daniel","last_name":"Capek","full_name":"Capek, Daniel"},{"first_name":"Sanjeev","last_name":"Galande","full_name":"Galande, Sanjeev"},{"orcid":"0000-0002-8023-9315","id":"33BA6C30-F248-11E8-B48F-1D18A9856A87","last_name":"Janovjak","first_name":"Harald L","full_name":"Janovjak, Harald L"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg"}],"related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"961"},{"id":"50","relation":"dissertation_contains","status":"public"}]},"date_created":"2018-12-11T11:50:08Z","date_updated":"2024-03-28T23:30:26Z","volume":16,"acknowledgement":"We are grateful to members of the C.-P.H. and H.J. labs for discussions, R. Hauschild and the different Scientific Service Units at IST Austria for technical help, M. Dravecka for performing initial experiments, A. Schier for reading an earlier version of the manuscript, K.W. Rogers for technical help, and C. Hill, A. Bruce, and L. Solnica-Krezel for sending plasmids. This work was supported by grants from the Austrian Science Foundation (FWF): (T560-B17) and (I 812-B12) to V.R. and C.-P.H., and from the European Union (EU FP7): (6275) to H.J. A.I.-P. is supported by a Ramon Areces fellowship.","year":"2016","publication_status":"published","department":[{"_id":"CaHe"},{"_id":"HaJa"}],"publisher":"Cell Press"},{"author":[{"first_name":"Paolo","last_name":"Maiuri","full_name":"Maiuri, Paolo"},{"full_name":"Rupprecht, Jean","last_name":"Rupprecht","first_name":"Jean"},{"last_name":"Wieser","first_name":"Stefan","orcid":"0000-0002-2670-2217","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","full_name":"Wieser, Stefan"},{"orcid":"0000-0003-4088-8633","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","last_name":"Ruprecht","first_name":"Verena","full_name":"Ruprecht, Verena"},{"first_name":"Olivier","last_name":"Bénichou","full_name":"Bénichou, Olivier"},{"first_name":"Nicolas","last_name":"Carpi","full_name":"Carpi, Nicolas"},{"last_name":"Coppey","first_name":"Mathieu","full_name":"Coppey, Mathieu"},{"full_name":"De Beco, Simon","first_name":"Simon","last_name":"De Beco"},{"full_name":"Gov, Nir","last_name":"Gov","first_name":"Nir"},{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"},{"first_name":"Carolina","last_name":"Lage Crespo","full_name":"Lage Crespo, Carolina"},{"full_name":"Lautenschlaeger, Franziska","first_name":"Franziska","last_name":"Lautenschlaeger"},{"full_name":"Le Berre, Maël","first_name":"Maël","last_name":"Le Berre"},{"full_name":"Lennon Duménil, Ana","first_name":"Ana","last_name":"Lennon Duménil"},{"full_name":"Raab, Matthew","first_name":"Matthew","last_name":"Raab"},{"full_name":"Thiam, Hawa","last_name":"Thiam","first_name":"Hawa"},{"full_name":"Piel, Matthieu","last_name":"Piel","first_name":"Matthieu"},{"first_name":"Michael K","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K"},{"full_name":"Voituriez, Raphaël","first_name":"Raphaël","last_name":"Voituriez"}],"oa_version":"None","volume":161,"date_updated":"2021-01-12T06:51:33Z","date_created":"2018-12-11T11:52:41Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"1553","year":"2015","publisher":"Cell Press","intvolume":" 161","department":[{"_id":"MiSi"},{"_id":"CaHe"}],"status":"public","publication_status":"published","title":"Actin flows mediate a universal coupling between cell speed and cell persistence","issue":"2","publist_id":"5618","ec_funded":1,"abstract":[{"lang":"eng","text":"Cell movement has essential functions in development, immunity, and cancer. Various cell migration patterns have been reported, but no general rule has emerged so far. Here, we show on the basis of experimental data in vitro and in vivo that cell persistence, which quantifies the straightness of trajectories, is robustly coupled to cell migration speed. We suggest that this universal coupling constitutes a generic law of cell migration, which originates in the advection of polarity cues by an actin cytoskeleton undergoing flows at the cellular scale. Our analysis relies on a theoretical model that we validate by measuring the persistence of cells upon modulation of actin flow speeds and upon optogenetic manipulation of the binding of an actin regulator to actin filaments. Beyond the quantitative prediction of the coupling, the model yields a generic phase diagram of cellular trajectories, which recapitulates the full range of observed migration patterns."}],"type":"journal_article","date_published":"2015-04-09T00:00:00Z","doi":"10.1016/j.cell.2015.01.056","language":[{"iso":"eng"}],"citation":{"apa":"Maiuri, P., Rupprecht, J., Wieser, S., Ruprecht, V., Bénichou, O., Carpi, N., … Voituriez, R. (2015). Actin flows mediate a universal coupling between cell speed and cell persistence. Cell. Cell Press. https://doi.org/10.1016/j.cell.2015.01.056","ieee":"P. Maiuri et al., “Actin flows mediate a universal coupling between cell speed and cell persistence,” Cell, vol. 161, no. 2. Cell Press, pp. 374–386, 2015.","ista":"Maiuri P, Rupprecht J, Wieser S, Ruprecht V, Bénichou O, Carpi N, Coppey M, De Beco S, Gov N, Heisenberg C-PJ, Lage Crespo C, Lautenschlaeger F, Le Berre M, Lennon Duménil A, Raab M, Thiam H, Piel M, Sixt MK, Voituriez R. 2015. Actin flows mediate a universal coupling between cell speed and cell persistence. Cell. 161(2), 374–386.","ama":"Maiuri P, Rupprecht J, Wieser S, et al. Actin flows mediate a universal coupling between cell speed and cell persistence. Cell. 2015;161(2):374-386. doi:10.1016/j.cell.2015.01.056","chicago":"Maiuri, Paolo, Jean Rupprecht, Stefan Wieser, Verena Ruprecht, Olivier Bénichou, Nicolas Carpi, Mathieu Coppey, et al. “Actin Flows Mediate a Universal Coupling between Cell Speed and Cell Persistence.” Cell. Cell Press, 2015. https://doi.org/10.1016/j.cell.2015.01.056.","short":"P. Maiuri, J. Rupprecht, S. Wieser, V. Ruprecht, O. Bénichou, N. Carpi, M. Coppey, S. De Beco, N. Gov, C.-P.J. Heisenberg, C. Lage Crespo, F. Lautenschlaeger, M. Le Berre, A. Lennon Duménil, M. Raab, H. Thiam, M. Piel, M.K. Sixt, R. Voituriez, Cell 161 (2015) 374–386.","mla":"Maiuri, Paolo, et al. “Actin Flows Mediate a Universal Coupling between Cell Speed and Cell Persistence.” Cell, vol. 161, no. 2, Cell Press, 2015, pp. 374–86, doi:10.1016/j.cell.2015.01.056."},"publication":"Cell","page":"374 - 386","project":[{"name":"Cell- and Tissue Mechanics in Zebrafish Germ Layer Formation","call_identifier":"FWF","grant_number":"T 560-B17","_id":"2529486C-B435-11E9-9278-68D0E5697425"},{"_id":"25A603A2-B435-11E9-9278-68D0E5697425","grant_number":"281556","name":"Cytoskeletal force generation and force transduction of migrating leukocytes (EU)","call_identifier":"FP7"},{"name":"Cell migration in complex environments: from in vivo experiments to theoretical models","_id":"25ABD200-B435-11E9-9278-68D0E5697425","grant_number":"RGP0058/2011"}],"quality_controlled":"1","day":"09","month":"04","scopus_import":1},{"scopus_import":"1","month":"04","day":"23","article_processing_charge":"No","publication":"Cell","citation":{"ama":"Bollenbach MT, Heisenberg C-PJ. Gradients are shaping up. Cell. 2015;161(3):431-432. doi:10.1016/j.cell.2015.04.009","ista":"Bollenbach MT, Heisenberg C-PJ. 2015. Gradients are shaping up. Cell. 161(3), 431–432.","ieee":"M. T. Bollenbach and C.-P. J. Heisenberg, “Gradients are shaping up,” Cell, vol. 161, no. 3. Cell Press, pp. 431–432, 2015.","apa":"Bollenbach, M. T., & Heisenberg, C.-P. J. (2015). Gradients are shaping up. Cell. Cell Press. https://doi.org/10.1016/j.cell.2015.04.009","mla":"Bollenbach, Mark Tobias, and Carl-Philipp J. Heisenberg. “Gradients Are Shaping Up.” Cell, vol. 161, no. 3, Cell Press, 2015, pp. 431–32, doi:10.1016/j.cell.2015.04.009.","short":"M.T. Bollenbach, C.-P.J. Heisenberg, Cell 161 (2015) 431–432.","chicago":"Bollenbach, Mark Tobias, and Carl-Philipp J Heisenberg. “Gradients Are Shaping Up.” Cell. Cell Press, 2015. https://doi.org/10.1016/j.cell.2015.04.009."},"quality_controlled":"1","page":"431 - 432","doi":"10.1016/j.cell.2015.04.009","date_published":"2015-04-23T00:00:00Z","language":[{"iso":"eng"}],"type":"journal_article","abstract":[{"lang":"eng","text":"In animal embryos, morphogen gradients determine tissue patterning and morphogenesis. Shyer et al. provide evidence that, during vertebrate gut formation, tissue folding generates graded activity of signals required for subsequent steps of gut growth and differentiation, thereby revealing an intriguing link between tissue morphogenesis and morphogen gradient formation."}],"issue":"3","publist_id":"5590","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","_id":"1581","year":"2015","title":"Gradients are shaping up","publication_status":"published","status":"public","intvolume":" 161","department":[{"_id":"ToBo"},{"_id":"CaHe"}],"publisher":"Cell Press","author":[{"full_name":"Bollenbach, Mark Tobias","last_name":"Bollenbach","first_name":"Mark Tobias","orcid":"0000-0003-4398-476X","id":"3E6DB97A-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg"}],"date_created":"2018-12-11T11:52:50Z","date_updated":"2022-08-25T13:56:10Z","oa_version":"None","volume":161},{"page":"217 - 221","publication":"Nature","citation":{"ama":"Porazinski S, Wang H, Asaoka Y, et al. YAP is essential for tissue tension to ensure vertebrate 3D body shape. Nature. 2015;521(7551):217-221. doi:10.1038/nature14215","ista":"Porazinski S, Wang H, Asaoka Y, Behrndt M, Miyamoto T, Morita H, Hata S, Sasaki T, Krens G, Osada Y, Asaka S, Momoi A, Linton S, Miesfeld J, Link B, Senga T, Castillo Morales A, Urrutia A, Shimizu N, Nagase H, Matsuura S, Bagby S, Kondoh H, Nishina H, Heisenberg C-PJ, Furutani Seiki M. 2015. YAP is essential for tissue tension to ensure vertebrate 3D body shape. Nature. 521(7551), 217–221.","ieee":"S. Porazinski et al., “YAP is essential for tissue tension to ensure vertebrate 3D body shape,” Nature, vol. 521, no. 7551. Nature Publishing Group, pp. 217–221, 2015.","apa":"Porazinski, S., Wang, H., Asaoka, Y., Behrndt, M., Miyamoto, T., Morita, H., … Furutani Seiki, M. (2015). YAP is essential for tissue tension to ensure vertebrate 3D body shape. Nature. Nature Publishing Group. https://doi.org/10.1038/nature14215","mla":"Porazinski, Sean, et al. “YAP Is Essential for Tissue Tension to Ensure Vertebrate 3D Body Shape.” Nature, vol. 521, no. 7551, Nature Publishing Group, 2015, pp. 217–21, doi:10.1038/nature14215.","short":"S. Porazinski, H. Wang, Y. Asaoka, M. Behrndt, T. Miyamoto, H. Morita, S. Hata, T. Sasaki, G. Krens, Y. Osada, S. Asaka, A. Momoi, S. Linton, J. Miesfeld, B. Link, T. Senga, A. Castillo Morales, A. Urrutia, N. Shimizu, H. Nagase, S. Matsuura, S. Bagby, H. Kondoh, H. Nishina, C.-P.J. Heisenberg, M. Furutani Seiki, Nature 521 (2015) 217–221.","chicago":"Porazinski, Sean, Huijia Wang, Yoichi Asaoka, Martin Behrndt, Tatsuo Miyamoto, Hitoshi Morita, Shoji Hata, et al. “YAP Is Essential for Tissue Tension to Ensure Vertebrate 3D Body Shape.” Nature. Nature Publishing Group, 2015. https://doi.org/10.1038/nature14215."},"date_published":"2015-03-16T00:00:00Z","scopus_import":1,"day":"16","title":"YAP is essential for tissue tension to ensure vertebrate 3D body shape","status":"public","intvolume":" 521","_id":"1817","user_id":"2EBD1598-F248-11E8-B48F-1D18A9856A87","oa_version":"Submitted Version","type":"journal_article","abstract":[{"text":"Vertebrates have a unique 3D body shape in which correct tissue and organ shape and alignment are essential for function. For example, vision requires the lens to be centred in the eye cup which must in turn be correctly positioned in the head. Tissue morphogenesis depends on force generation, force transmission through the tissue, and response of tissues and extracellular matrix to force. Although a century ago D'Arcy Thompson postulated that terrestrial animal body shapes are conditioned by gravity, there has been no animal model directly demonstrating how the aforementioned mechano-morphogenetic processes are coordinated to generate a body shape that withstands gravity. Here we report a unique medaka fish (Oryzias latipes) mutant, hirame (hir), which is sensitive to deformation by gravity. hir embryos display a markedly flattened body caused by mutation of YAP, a nuclear executor of Hippo signalling that regulates organ size. We show that actomyosin-mediated tissue tension is reduced in hir embryos, leading to tissue flattening and tissue misalignment, both of which contribute to body flattening. By analysing YAP function in 3D spheroids of human cells, we identify the Rho GTPase activating protein ARHGAP18 as an effector of YAP in controlling tissue tension. Together, these findings reveal a previously unrecognised function of YAP in regulating tissue shape and alignment required for proper 3D body shape. Understanding this morphogenetic function of YAP could facilitate the use of embryonic stem cells to generate complex organs requiring correct alignment of multiple tissues. ","lang":"eng"}],"issue":"7551","quality_controlled":"1","external_id":{"pmid":["25778702"]},"main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4720436/","open_access":"1"}],"oa":1,"language":[{"iso":"eng"}],"doi":"10.1038/nature14215","month":"03","publication_status":"published","department":[{"_id":"CaHe"}],"publisher":"Nature Publishing Group","year":"2015","pmid":1,"date_created":"2018-12-11T11:54:10Z","date_updated":"2021-01-12T06:53:23Z","volume":521,"author":[{"last_name":"Porazinski","first_name":"Sean","full_name":"Porazinski, Sean"},{"full_name":"Wang, Huijia","first_name":"Huijia","last_name":"Wang"},{"full_name":"Asaoka, Yoichi","last_name":"Asaoka","first_name":"Yoichi"},{"full_name":"Behrndt, Martin","last_name":"Behrndt","first_name":"Martin","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Miyamoto","first_name":"Tatsuo","full_name":"Miyamoto, Tatsuo"},{"id":"4C6E54C6-F248-11E8-B48F-1D18A9856A87","first_name":"Hitoshi","last_name":"Morita","full_name":"Morita, Hitoshi"},{"full_name":"Hata, Shoji","last_name":"Hata","first_name":"Shoji"},{"last_name":"Sasaki","first_name":"Takashi","full_name":"Sasaki, Takashi"},{"full_name":"Krens, Gabriel","first_name":"Gabriel","last_name":"Krens","id":"2B819732-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4761-5996"},{"full_name":"Osada, Yumi","last_name":"Osada","first_name":"Yumi"},{"full_name":"Asaka, Satoshi","first_name":"Satoshi","last_name":"Asaka"},{"first_name":"Akihiro","last_name":"Momoi","full_name":"Momoi, Akihiro"},{"full_name":"Linton, Sarah","last_name":"Linton","first_name":"Sarah"},{"last_name":"Miesfeld","first_name":"Joel","full_name":"Miesfeld, Joel"},{"full_name":"Link, Brian","first_name":"Brian","last_name":"Link"},{"first_name":"Takeshi","last_name":"Senga","full_name":"Senga, Takeshi"},{"full_name":"Castillo Morales, Atahualpa","last_name":"Castillo Morales","first_name":"Atahualpa"},{"full_name":"Urrutia, Araxi","first_name":"Araxi","last_name":"Urrutia"},{"last_name":"Shimizu","first_name":"Nobuyoshi","full_name":"Shimizu, Nobuyoshi"},{"first_name":"Hideaki","last_name":"Nagase","full_name":"Nagase, Hideaki"},{"first_name":"Shinya","last_name":"Matsuura","full_name":"Matsuura, Shinya"},{"full_name":"Bagby, Stefan","first_name":"Stefan","last_name":"Bagby"},{"full_name":"Kondoh, Hisato","first_name":"Hisato","last_name":"Kondoh"},{"full_name":"Nishina, Hiroshi","first_name":"Hiroshi","last_name":"Nishina"},{"last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J"},{"last_name":"Furutani Seiki","first_name":"Makoto","full_name":"Furutani Seiki, Makoto"}],"publist_id":"5289"},{"day":"01","month":"12","scopus_import":1,"date_published":"2015-12-01T00:00:00Z","doi":"10.1093/glycob/cwv059","language":[{"iso":"eng"}],"publication":"Glycobiology","external_id":{"pmid":["26306635"]},"citation":{"chicago":"Engel, Jakob, Philipp S Schmalhorst, Anke Kruger, Christina Muller, Falk Buettner, and Françoise Routier. “Characterization of an N-Acetylglucosaminyltransferase Involved in Aspergillus Fumigatus Zwitterionic Glycoinositolphosphoceramide Biosynthesis.” Glycobiology. Oxford University Press, 2015. https://doi.org/10.1093/glycob/cwv059.","short":"J. Engel, P.S. Schmalhorst, A. Kruger, C. Muller, F. Buettner, F. Routier, Glycobiology 25 (2015) 1423–1430.","mla":"Engel, Jakob, et al. “Characterization of an N-Acetylglucosaminyltransferase Involved in Aspergillus Fumigatus Zwitterionic Glycoinositolphosphoceramide Biosynthesis.” Glycobiology, vol. 25, no. 12, Oxford University Press, 2015, pp. 1423–30, doi:10.1093/glycob/cwv059.","apa":"Engel, J., Schmalhorst, P. S., Kruger, A., Muller, C., Buettner, F., & Routier, F. (2015). Characterization of an N-acetylglucosaminyltransferase involved in Aspergillus fumigatus zwitterionic glycoinositolphosphoceramide biosynthesis. Glycobiology. Oxford University Press. https://doi.org/10.1093/glycob/cwv059","ieee":"J. Engel, P. S. Schmalhorst, A. Kruger, C. Muller, F. Buettner, and F. Routier, “Characterization of an N-acetylglucosaminyltransferase involved in Aspergillus fumigatus zwitterionic glycoinositolphosphoceramide biosynthesis,” Glycobiology, vol. 25, no. 12. Oxford University Press, pp. 1423–1430, 2015.","ista":"Engel J, Schmalhorst PS, Kruger A, Muller C, Buettner F, Routier F. 2015. Characterization of an N-acetylglucosaminyltransferase involved in Aspergillus fumigatus zwitterionic glycoinositolphosphoceramide biosynthesis. Glycobiology. 25(12), 1423–1430.","ama":"Engel J, Schmalhorst PS, Kruger A, Muller C, Buettner F, Routier F. Characterization of an N-acetylglucosaminyltransferase involved in Aspergillus fumigatus zwitterionic glycoinositolphosphoceramide biosynthesis. Glycobiology. 2015;25(12):1423-1430. doi:10.1093/glycob/cwv059"},"quality_controlled":"1","page":"1423 - 1430","abstract":[{"lang":"eng","text":"Glycoinositolphosphoceramides (GIPCs) are complex sphingolipids present at the plasma membrane of various eukaryotes with the important exception of mammals. In fungi, these glycosphingolipids commonly contain an alpha-mannose residue (Man) linked at position 2 of the inositol. However, several pathogenic fungi additionally synthesize zwitterionic GIPCs carrying an alpha-glucosamine residue (GlcN) at this position. In the human pathogen Aspergillus fumigatus, the GlcNalpha1,2IPC core (where IPC is inositolphosphoceramide) is elongated to Manalpha1,3Manalpha1,6GlcNalpha1,2IPC, which is the most abundant GIPC synthesized by this fungus. In this study, we identified an A. fumigatus N-acetylglucosaminyltransferase, named GntA, and demonstrate its involvement in the initiation of zwitterionic GIPC biosynthesis. Targeted deletion of the gene encoding GntA in A. fumigatus resulted in complete absence of zwitterionic GIPC; a phenotype that could be reverted by episomal expression of GntA in the mutant. The N-acetylhexosaminyltransferase activity of GntA was substantiated by production of N-acetylhexosamine-IPC in the yeast Saccharomyces cerevisiae upon GntA expression. Using an in vitro assay, GntA was furthermore shown to use UDP-N-acetylglucosamine as donor substrate to generate a glycolipid product resistant to saponification and to digestion by phosphatidylinositol-phospholipase C as expected for GlcNAcalpha1,2IPC. Finally, as the enzymes involved in mannosylation of IPC, GntA was localized to the Golgi apparatus, the site of IPC synthesis."}],"publist_id":"6851","issue":"12","type":"journal_article","author":[{"full_name":"Engel, Jakob","first_name":"Jakob","last_name":"Engel"},{"full_name":"Schmalhorst, Philipp S","last_name":"Schmalhorst","first_name":"Philipp S","orcid":"0000-0002-5795-0133","id":"309D50DA-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Kruger, Anke","last_name":"Kruger","first_name":"Anke"},{"first_name":"Christina","last_name":"Muller","full_name":"Muller, Christina"},{"last_name":"Buettner","first_name":"Falk","full_name":"Buettner, Falk"},{"full_name":"Routier, Françoise","first_name":"Françoise","last_name":"Routier"}],"date_created":"2018-12-11T11:48:35Z","date_updated":"2021-01-12T08:16:33Z","oa_version":"None","volume":25,"_id":"802","year":"2015","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pmid":1,"status":"public","title":"Characterization of an N-acetylglucosaminyltransferase involved in Aspergillus fumigatus zwitterionic glycoinositolphosphoceramide biosynthesis","publication_status":"published","intvolume":" 25","department":[{"_id":"CaHe"}],"publisher":"Oxford University Press"},{"type":"journal_article","issue":"10","abstract":[{"text":"Deposits of misfolded proteins in the human brain are associated with the development of many neurodegenerative diseases. Recent studies show that these proteins have common traits even at the monomer level. Among them, a polyglutamine region that is present in huntingtin is known to exhibit a correlation between the length of the chain and the severity as well as the earliness of the onset of Huntington disease. Here, we apply bias exchange molecular dynamics to generate structures of polyglutamine expansions of several lengths and characterize the resulting independent conformations. We compare the properties of these conformations to those of the standard proteins, as well as to other homopolymeric tracts. We find that, similar to the previously studied polyvaline chains, the set of possible transient folds is much broader than the set of known-to-date folds, although the conformations have different structures. We show that the mechanical stability is not related to any simple geometrical characteristics of the structures. We demonstrate that long polyglutamine expansions result in higher mechanical stability than the shorter ones. They also have a longer life span and are substantially more prone to form knotted structures. The knotted region has an average length of 35 residues, similar to the typical threshold for most polyglutamine-related diseases. Similarly, changes in shape and mechanical stability appear once the total length of the peptide exceeds this threshold of 35 glutamine residues. We suggest that knotted conformers may also harm the cellular machinery and thus lead to disease.","lang":"eng"}],"intvolume":" 11","title":"An exploration of the universe of polyglutamine structures","status":"public","ddc":["570"],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"1566","file":[{"creator":"system","file_size":1412511,"content_type":"application/pdf","file_name":"IST-2016-478-v1+1_journal.pcbi.1004541.pdf","access_level":"open_access","date_updated":"2020-07-14T12:45:02Z","date_created":"2018-12-12T10:16:21Z","checksum":"8b67d729be663bfc9af04bfd94459655","file_id":"5207","relation":"main_file"}],"oa_version":"Published Version","pubrep_id":"478","scopus_import":1,"has_accepted_license":"1","day":"23","citation":{"ieee":"À. Gómez Sicilia, M. K. Sikora, M. Cieplak, and M. Carrión Vázquez, “An exploration of the universe of polyglutamine structures,” PLoS Computational Biology, vol. 11, no. 10. Public Library of Science, 2015.","apa":"Gómez Sicilia, À., Sikora, M. K., Cieplak, M., & Carrión Vázquez, M. (2015). An exploration of the universe of polyglutamine structures. PLoS Computational Biology. Public Library of Science. https://doi.org/10.1371/journal.pcbi.1004541","ista":"Gómez Sicilia À, Sikora MK, Cieplak M, Carrión Vázquez M. 2015. An exploration of the universe of polyglutamine structures. PLoS Computational Biology. 11(10), e1004541.","ama":"Gómez Sicilia À, Sikora MK, Cieplak M, Carrión Vázquez M. An exploration of the universe of polyglutamine structures. PLoS Computational Biology. 2015;11(10). doi:10.1371/journal.pcbi.1004541","chicago":"Gómez Sicilia, Àngel, Mateusz K Sikora, Marek Cieplak, and Mariano Carrión Vázquez. “An Exploration of the Universe of Polyglutamine Structures.” PLoS Computational Biology. Public Library of Science, 2015. https://doi.org/10.1371/journal.pcbi.1004541.","short":"À. Gómez Sicilia, M.K. Sikora, M. Cieplak, M. Carrión Vázquez, PLoS Computational Biology 11 (2015).","mla":"Gómez Sicilia, Àngel, et al. “An Exploration of the Universe of Polyglutamine Structures.” PLoS Computational Biology, vol. 11, no. 10, e1004541, Public Library of Science, 2015, doi:10.1371/journal.pcbi.1004541."},"publication":"PLoS Computational Biology","date_published":"2015-10-23T00:00:00Z","article_number":"e1004541","publist_id":"5605","file_date_updated":"2020-07-14T12:45:02Z","publisher":"Public Library of Science","department":[{"_id":"CaHe"}],"publication_status":"published","acknowledgement":"We acknowledge the support by the EU Joint Programme in Neurodegenerative Diseases (JPND AC14/00037) project. The project is supported through the following funding organisations under the aegis of JPND—www.jpnd.eu: Ireland, HRB; Poland, National Science Centre; and Spain, ISCIII. ","year":"2015","volume":11,"date_updated":"2023-02-23T14:05:55Z","date_created":"2018-12-11T11:52:45Z","related_material":{"record":[{"id":"9714","relation":"research_data","status":"public"}]},"author":[{"full_name":"Gómez Sicilia, Àngel","first_name":"Àngel","last_name":"Gómez Sicilia"},{"full_name":"Sikora, Mateusz K","last_name":"Sikora","first_name":"Mateusz K","id":"2F74BCDE-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Cieplak, Marek","first_name":"Marek","last_name":"Cieplak"},{"full_name":"Carrión Vázquez, Mariano","last_name":"Carrión Vázquez","first_name":"Mariano"}],"month":"10","quality_controlled":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"language":[{"iso":"eng"}],"doi":"10.1371/journal.pcbi.1004541"},{"type":"research_data_reference","author":[{"full_name":"Gómez Sicilia, Àngel","first_name":"Àngel","last_name":"Gómez Sicilia"},{"full_name":"Sikora, Mateusz K","id":"2F74BCDE-F248-11E8-B48F-1D18A9856A87","last_name":"Sikora","first_name":"Mateusz K"},{"first_name":"Marek","last_name":"Cieplak","full_name":"Cieplak, Marek"},{"last_name":"Carrión Vázquez","first_name":"Mariano","full_name":"Carrión Vázquez, Mariano"}],"related_material":{"record":[{"relation":"used_in_publication","status":"public","id":"1566"}]},"date_created":"2021-07-23T12:05:28Z","date_updated":"2023-02-23T10:04:35Z","oa_version":"Published Version","user_id":"6785fbc1-c503-11eb-8a32-93094b40e1cf","_id":"9714","year":"2015","title":"An exploration of the universe of polyglutamine structures - submission to PLOS journals","status":"public","department":[{"_id":"CaHe"}],"publisher":"Public Library of Science ","day":"23","month":"10","article_processing_charge":"No","date_published":"2015-10-23T00:00:00Z","doi":"10.1371/journal.pcbi.1004541.s001","citation":{"chicago":"Gómez Sicilia, Àngel, Mateusz K Sikora, Marek Cieplak, and Mariano Carrión Vázquez. “An Exploration of the Universe of Polyglutamine Structures - Submission to PLOS Journals.” Public Library of Science , 2015. https://doi.org/10.1371/journal.pcbi.1004541.s001.","mla":"Gómez Sicilia, Àngel, et al. An Exploration of the Universe of Polyglutamine Structures - Submission to PLOS Journals. Public Library of Science , 2015, doi:10.1371/journal.pcbi.1004541.s001.","short":"À. Gómez Sicilia, M.K. Sikora, M. Cieplak, M. Carrión Vázquez, (2015).","ista":"Gómez Sicilia À, Sikora MK, Cieplak M, Carrión Vázquez M. 2015. An exploration of the universe of polyglutamine structures - submission to PLOS journals, Public Library of Science , 10.1371/journal.pcbi.1004541.s001.","apa":"Gómez Sicilia, À., Sikora, M. K., Cieplak, M., & Carrión Vázquez, M. (2015). An exploration of the universe of polyglutamine structures - submission to PLOS journals. Public Library of Science . https://doi.org/10.1371/journal.pcbi.1004541.s001","ieee":"À. Gómez Sicilia, M. K. Sikora, M. Cieplak, and M. Carrión Vázquez, “An exploration of the universe of polyglutamine structures - submission to PLOS journals.” Public Library of Science , 2015.","ama":"Gómez Sicilia À, Sikora MK, Cieplak M, Carrión Vázquez M. An exploration of the universe of polyglutamine structures - submission to PLOS journals. 2015. doi:10.1371/journal.pcbi.1004541.s001"}},{"day":"12","has_accepted_license":"1","scopus_import":1,"date_published":"2015-02-12T00:00:00Z","publication":"Cell","citation":{"ama":"Ruprecht V, Wieser S, Callan Jones A, et al. Cortical contractility triggers a stochastic switch to fast amoeboid cell motility. Cell. 2015;160(4):673-685. doi:10.1016/j.cell.2015.01.008","ista":"Ruprecht V, Wieser S, Callan Jones A, Smutny M, Morita H, Sako K, Barone V, Ritsch Marte M, Sixt MK, Voituriez R, Heisenberg C-PJ. 2015. Cortical contractility triggers a stochastic switch to fast amoeboid cell motility. Cell. 160(4), 673–685.","ieee":"V. Ruprecht et al., “Cortical contractility triggers a stochastic switch to fast amoeboid cell motility,” Cell, vol. 160, no. 4. Cell Press, pp. 673–685, 2015.","apa":"Ruprecht, V., Wieser, S., Callan Jones, A., Smutny, M., Morita, H., Sako, K., … Heisenberg, C.-P. J. (2015). Cortical contractility triggers a stochastic switch to fast amoeboid cell motility. Cell. Cell Press. https://doi.org/10.1016/j.cell.2015.01.008","mla":"Ruprecht, Verena, et al. “Cortical Contractility Triggers a Stochastic Switch to Fast Amoeboid Cell Motility.” Cell, vol. 160, no. 4, Cell Press, 2015, pp. 673–85, doi:10.1016/j.cell.2015.01.008.","short":"V. Ruprecht, S. Wieser, A. Callan Jones, M. Smutny, H. Morita, K. Sako, V. Barone, M. Ritsch Marte, M.K. Sixt, R. Voituriez, C.-P.J. Heisenberg, Cell 160 (2015) 673–685.","chicago":"Ruprecht, Verena, Stefan Wieser, Andrew Callan Jones, Michael Smutny, Hitoshi Morita, Keisuke Sako, Vanessa Barone, et al. “Cortical Contractility Triggers a Stochastic Switch to Fast Amoeboid Cell Motility.” Cell. Cell Press, 2015. https://doi.org/10.1016/j.cell.2015.01.008."},"page":"673 - 685","abstract":[{"text":"3D amoeboid cell migration is central to many developmental and disease-related processes such as cancer metastasis. Here, we identify a unique prototypic amoeboid cell migration mode in early zebrafish embryos, termed stable-bleb migration. Stable-bleb cells display an invariant polarized balloon-like shape with exceptional migration speed and persistence. Progenitor cells can be reversibly transformed into stable-bleb cells irrespective of their primary fate and motile characteristics by increasing myosin II activity through biochemical or mechanical stimuli. Using a combination of theory and experiments, we show that, in stable-bleb cells, cortical contractility fluctuations trigger a stochastic switch into amoeboid motility, and a positive feedback between cortical flows and gradients in contractility maintains stable-bleb cell polarization. We further show that rearward cortical flows drive stable-bleb cell migration in various adhesive and non-adhesive environments, unraveling a highly versatile amoeboid migration phenotype.","lang":"eng"}],"issue":"4","type":"journal_article","pubrep_id":"484","file":[{"checksum":"228d3edf40627d897b3875088a0ac51f","date_created":"2018-12-12T10:13:21Z","date_updated":"2020-07-14T12:45:01Z","file_id":"5003","relation":"main_file","creator":"system","content_type":"application/pdf","file_size":4362653,"access_level":"open_access","file_name":"IST-2016-484-v1+1_1-s2.0-S0092867415000094-main.pdf"}],"oa_version":"Published Version","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","_id":"1537","status":"public","title":"Cortical contractility triggers a stochastic switch to fast amoeboid cell motility","ddc":["570"],"intvolume":" 160","month":"02","doi":"10.1016/j.cell.2015.01.008","acknowledged_ssus":[{"_id":"SSU"}],"language":[{"iso":"eng"}],"oa":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"quality_controlled":"1","project":[{"call_identifier":"FWF","name":"Cell- and Tissue Mechanics in Zebrafish Germ Layer Formation","_id":"2529486C-B435-11E9-9278-68D0E5697425","grant_number":"T 560-B17"},{"_id":"2527D5CC-B435-11E9-9278-68D0E5697425","grant_number":"I 812-B12","call_identifier":"FWF","name":"Cell Cortex and Germ Layer Formation in Zebrafish Gastrulation"}],"file_date_updated":"2020-07-14T12:45:01Z","publist_id":"5634","author":[{"full_name":"Ruprecht, Verena","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4088-8633","first_name":"Verena","last_name":"Ruprecht"},{"last_name":"Wieser","first_name":"Stefan","orcid":"0000-0002-2670-2217","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","full_name":"Wieser, Stefan"},{"full_name":"Callan Jones, Andrew","first_name":"Andrew","last_name":"Callan Jones"},{"id":"3FE6E4E8-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5920-9090","first_name":"Michael","last_name":"Smutny","full_name":"Smutny, Michael"},{"id":"4C6E54C6-F248-11E8-B48F-1D18A9856A87","first_name":"Hitoshi","last_name":"Morita","full_name":"Morita, Hitoshi"},{"full_name":"Sako, Keisuke","last_name":"Sako","first_name":"Keisuke","orcid":"0000-0002-6453-8075","id":"3BED66BE-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Barone, Vanessa","last_name":"Barone","first_name":"Vanessa","orcid":"0000-0003-2676-3367","id":"419EECCC-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Ritsch Marte, Monika","last_name":"Ritsch Marte","first_name":"Monika"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","first_name":"Michael K","last_name":"Sixt","full_name":"Sixt, Michael K"},{"full_name":"Voituriez, Raphaël","first_name":"Raphaël","last_name":"Voituriez"},{"last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J"}],"related_material":{"record":[{"id":"961","status":"public","relation":"dissertation_contains"}]},"date_created":"2018-12-11T11:52:35Z","date_updated":"2023-09-07T12:05:08Z","volume":160,"acknowledgement":"We would like to thank R. Hausschild and E. Papusheva for technical assistance and the service facilities at the IST Austria for continuous support. The caRhoA plasmid was a kind gift of T. Kudoh and A. Takesono. We thank M. Piel and E. Paluch for exchanging unpublished data. ","year":"2015","publication_status":"published","publisher":"Cell Press","department":[{"_id":"CaHe"},{"_id":"MiSi"}]},{"publication_status":"published","department":[{"_id":"CaHe"}],"publisher":"Wiley","acknowledgement":"The authors thank all the members of the Division of Morphogenesis, National Institute for Basic Biology, for their contributions to the research, their encouragement, and helpful discussions, particularly Dr M. Suzuki for his critical reading of the manuscript. We also thank the Model Animal Research and Spectrography and Bioimaging Facilities, NIBB Core Research Facilities, for technical support. M.H. was supported by a research fellowship from the Japan Society for the Promotion of Science (JSPS). Our work introduced in this review was supported by a Grant-in-Aid for Scientific Research on Innovative Areas from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan, to N.U.","year":"2014","pmid":1,"date_updated":"2022-03-04T08:26:05Z","date_created":"2022-03-04T08:17:25Z","volume":54,"author":[{"last_name":"Hashimoto","first_name":"Masakazu","full_name":"Hashimoto, Masakazu"},{"full_name":"Morita, Hitoshi","id":"4C6E54C6-F248-11E8-B48F-1D18A9856A87","first_name":"Hitoshi","last_name":"Morita"},{"first_name":"Naoto","last_name":"Ueno","full_name":"Ueno, Naoto"}],"month":"02","publication_identifier":{"issn":["0914-3505"]},"quality_controlled":"1","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1111/cga.12039"}],"external_id":{"pmid":["24666178"]},"oa":1,"language":[{"iso":"eng"}],"doi":"10.1111/cga.12039","type":"journal_article","abstract":[{"lang":"eng","text":"In the last several decades, developmental biology has clarified the molecular mechanisms of embryogenesis and organogenesis. In particular, it has demonstrated that the “tool-kit genes” essential for regulating developmental processes are not only highly conserved among species, but are also used as systems at various times and places in an organism to control distinct developmental events. Therefore, mutations in many of these tool-kit genes may cause congenital diseases involving morphological abnormalities. This link between genes and abnormal morphological phenotypes underscores the importance of understanding how cells behave and contribute to morphogenesis as a result of gene function. Recent improvements in live imaging and in quantitative analyses of cellular dynamics will advance our understanding of the cellular pathogenesis of congenital diseases associated with aberrant morphologies. In these studies, it is critical to select an appropriate model organism for the particular phenomenon of interest."}],"issue":"1","status":"public","title":"Molecular and cellular mechanisms of development underlying congenital diseases","intvolume":" 54","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"10815","oa_version":"None","keyword":["Developmental Biology","Embryology","General Medicine","Pediatrics","Perinatology","and Child Health"],"scopus_import":"1","day":"01","article_processing_charge":"No","article_type":"original","page":"1-7","publication":"Congenital Anomalies","citation":{"mla":"Hashimoto, Masakazu, et al. “Molecular and Cellular Mechanisms of Development Underlying Congenital Diseases.” Congenital Anomalies, vol. 54, no. 1, Wiley, 2014, pp. 1–7, doi:10.1111/cga.12039.","short":"M. Hashimoto, H. Morita, N. Ueno, Congenital Anomalies 54 (2014) 1–7.","chicago":"Hashimoto, Masakazu, Hitoshi Morita, and Naoto Ueno. “Molecular and Cellular Mechanisms of Development Underlying Congenital Diseases.” Congenital Anomalies. Wiley, 2014. https://doi.org/10.1111/cga.12039.","ama":"Hashimoto M, Morita H, Ueno N. Molecular and cellular mechanisms of development underlying congenital diseases. Congenital Anomalies. 2014;54(1):1-7. doi:10.1111/cga.12039","ista":"Hashimoto M, Morita H, Ueno N. 2014. Molecular and cellular mechanisms of development underlying congenital diseases. Congenital Anomalies. 54(1), 1–7.","ieee":"M. Hashimoto, H. Morita, and N. Ueno, “Molecular and cellular mechanisms of development underlying congenital diseases,” Congenital Anomalies, vol. 54, no. 1. Wiley, pp. 1–7, 2014.","apa":"Hashimoto, M., Morita, H., & Ueno, N. (2014). Molecular and cellular mechanisms of development underlying congenital diseases. Congenital Anomalies. Wiley. https://doi.org/10.1111/cga.12039"},"date_published":"2014-02-01T00:00:00Z"},{"abstract":[{"lang":"eng","text":"We provide theoretical tests of a novel experimental technique to determine mechanostability of proteins based on stretching a mechanically protected protein by single-molecule force spectroscopy. This technique involves stretching a homogeneous or heterogeneous chain of reference proteins (single-molecule markers) in which one of them acts as host to the guest protein under study. The guest protein is grafted into the host through genetic engineering. It is expected that unraveling of the host precedes the unraveling of the guest removing ambiguities in the reading of the force-extension patterns of the guest protein. We study examples of such systems within a coarse-grained structure-based model. We consider systems with various ratios of mechanostability for the host and guest molecules and compare them to experimental results involving cohesin I as the guest molecule. For a comparison, we also study the force-displacement patterns in proteins that are linked in a serial fashion. We find that the mechanostability of the guest is similar to that of the isolated or serially linked protein. We also demonstrate that the ideal configuration of this strategy would be one in which the host is much more mechanostable than the single-molecule markers. We finally show that it is troublesome to use the highly stable cystine knot proteins as a host to graft a guest in stretching studies because this would involve a cleaving procedure."}],"issue":"5","publist_id":"5204","type":"journal_article","date_updated":"2021-01-12T06:53:52Z","date_created":"2018-12-11T11:54:34Z","oa_version":"None","volume":82,"author":[{"full_name":"Chwastyk, Mateusz","last_name":"Chwastyk","first_name":"Mateusz"},{"full_name":"Galera Prat, Albert","first_name":"Albert","last_name":"Galera Prat"},{"id":"2F74BCDE-F248-11E8-B48F-1D18A9856A87","last_name":"Sikora","first_name":"Mateusz K","full_name":"Sikora, Mateusz K"},{"last_name":"Gómez Sicilia","first_name":"Àngel","full_name":"Gómez Sicilia, Àngel"},{"full_name":"Carrión Vázquez, Mariano","first_name":"Mariano","last_name":"Carrión Vázquez"},{"full_name":"Cieplak, Marek","first_name":"Marek","last_name":"Cieplak"}],"status":"public","publication_status":"published","title":"Theoretical tests of the mechanical protection strategy in protein nanomechanics","publisher":"Wiley-Blackwell","intvolume":" 82","department":[{"_id":"CaHe"}],"user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","_id":"1891","year":"2014","acknowledgement":"Grant Nr. 2011/01/N/ST3/02475","month":"05","day":"01","scopus_import":1,"language":[{"iso":"eng"}],"doi":"10.1002/prot.24436","date_published":"2014-05-01T00:00:00Z","page":"717 - 726","publication":"Proteins: Structure, Function and Bioinformatics","citation":{"chicago":"Chwastyk, Mateusz, Albert Galera Prat, Mateusz K Sikora, Àngel Gómez Sicilia, Mariano Carrión Vázquez, and Marek Cieplak. “Theoretical Tests of the Mechanical Protection Strategy in Protein Nanomechanics.” Proteins: Structure, Function and Bioinformatics. Wiley-Blackwell, 2014. https://doi.org/10.1002/prot.24436.","mla":"Chwastyk, Mateusz, et al. “Theoretical Tests of the Mechanical Protection Strategy in Protein Nanomechanics.” Proteins: Structure, Function and Bioinformatics, vol. 82, no. 5, Wiley-Blackwell, 2014, pp. 717–26, doi:10.1002/prot.24436.","short":"M. Chwastyk, A. Galera Prat, M.K. Sikora, À. Gómez Sicilia, M. Carrión Vázquez, M. Cieplak, Proteins: Structure, Function and Bioinformatics 82 (2014) 717–726.","ista":"Chwastyk M, Galera Prat A, Sikora MK, Gómez Sicilia À, Carrión Vázquez M, Cieplak M. 2014. Theoretical tests of the mechanical protection strategy in protein nanomechanics. Proteins: Structure, Function and Bioinformatics. 82(5), 717–726.","apa":"Chwastyk, M., Galera Prat, A., Sikora, M. K., Gómez Sicilia, À., Carrión Vázquez, M., & Cieplak, M. (2014). Theoretical tests of the mechanical protection strategy in protein nanomechanics. Proteins: Structure, Function and Bioinformatics. Wiley-Blackwell. https://doi.org/10.1002/prot.24436","ieee":"M. Chwastyk, A. Galera Prat, M. K. Sikora, À. Gómez Sicilia, M. Carrión Vázquez, and M. Cieplak, “Theoretical tests of the mechanical protection strategy in protein nanomechanics,” Proteins: Structure, Function and Bioinformatics, vol. 82, no. 5. Wiley-Blackwell, pp. 717–726, 2014.","ama":"Chwastyk M, Galera Prat A, Sikora MK, Gómez Sicilia À, Carrión Vázquez M, Cieplak M. Theoretical tests of the mechanical protection strategy in protein nanomechanics. Proteins: Structure, Function and Bioinformatics. 2014;82(5):717-726. doi:10.1002/prot.24436"}},{"author":[{"full_name":"Behrndt, Martin","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87","first_name":"Martin","last_name":"Behrndt"},{"full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J"}],"date_updated":"2021-01-12T06:53:56Z","date_created":"2018-12-11T11:54:37Z","oa_version":"None","volume":16,"year":"2014","_id":"1900","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","publication_status":"published","status":"public","title":"Lateral junction dynamics lead the way out","department":[{"_id":"CaHe"}],"intvolume":" 16","publisher":"Nature Publishing Group","abstract":[{"lang":"eng","text":"Epithelial cell layers need to be tightly regulated to maintain their integrity and correct function. Cell integration into epithelial sheets is now shown to depend on the N-WASP-regulated stabilization of cortical F-actin, which generates distinct patterns of apical-lateral contractility at E-cadherin-based cell-cell junctions."}],"issue":"2","publist_id":"5195","type":"journal_article","date_published":"2014-01-31T00:00:00Z","doi":"10.1038/ncb2913","language":[{"iso":"eng"}],"publication":"Nature Cell Biology","citation":{"ama":"Behrndt M, Heisenberg C-PJ. Lateral junction dynamics lead the way out. Nature Cell Biology. 2014;16(2):127-129. doi:10.1038/ncb2913","ista":"Behrndt M, Heisenberg C-PJ. 2014. Lateral junction dynamics lead the way out. Nature Cell Biology. 16(2), 127–129.","apa":"Behrndt, M., & Heisenberg, C.-P. J. (2014). Lateral junction dynamics lead the way out. Nature Cell Biology. Nature Publishing Group. https://doi.org/10.1038/ncb2913","ieee":"M. Behrndt and C.-P. J. Heisenberg, “Lateral junction dynamics lead the way out,” Nature Cell Biology, vol. 16, no. 2. Nature Publishing Group, pp. 127–129, 2014.","mla":"Behrndt, Martin, and Carl-Philipp J. Heisenberg. “Lateral Junction Dynamics Lead the Way Out.” Nature Cell Biology, vol. 16, no. 2, Nature Publishing Group, 2014, pp. 127–29, doi:10.1038/ncb2913.","short":"M. Behrndt, C.-P.J. Heisenberg, Nature Cell Biology 16 (2014) 127–129.","chicago":"Behrndt, Martin, and Carl-Philipp J Heisenberg. “Lateral Junction Dynamics Lead the Way Out.” Nature Cell Biology. Nature Publishing Group, 2014. https://doi.org/10.1038/ncb2913."},"quality_controlled":"1","page":"127 - 129","day":"31","month":"01","scopus_import":1},{"scopus_import":1,"has_accepted_license":"1","article_processing_charge":"No","day":"28","citation":{"ieee":"C. Lamprecht et al., “A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes,” Nanotechnology, vol. 25, no. 12. IOP Publishing, 2014.","apa":"Lamprecht, C., Plochberger, B., Ruprecht, V., Wieser, S., Rankl, C., Heister, E., … Ebner, A. (2014). A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes. Nanotechnology. IOP Publishing. https://doi.org/10.1088/0957-4484/25/12/125704","ista":"Lamprecht C, Plochberger B, Ruprecht V, Wieser S, Rankl C, Heister E, Unterauer B, Brameshuber M, Danzberger J, Lukanov P, Flahaut E, Schütz G, Hinterdorfer P, Ebner A. 2014. A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes. Nanotechnology. 25(12), 125704.","ama":"Lamprecht C, Plochberger B, Ruprecht V, et al. A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes. Nanotechnology. 2014;25(12). doi:10.1088/0957-4484/25/12/125704","chicago":"Lamprecht, Constanze, Birgit Plochberger, Verena Ruprecht, Stefan Wieser, Christian Rankl, Elena Heister, Barbara Unterauer, et al. “A Single-Molecule Approach to Explore Binding Uptake and Transport of Cancer Cell Targeting Nanotubes.” Nanotechnology. IOP Publishing, 2014. https://doi.org/10.1088/0957-4484/25/12/125704.","short":"C. Lamprecht, B. Plochberger, V. Ruprecht, S. Wieser, C. Rankl, E. Heister, B. Unterauer, M. Brameshuber, J. Danzberger, P. Lukanov, E. Flahaut, G. Schütz, P. Hinterdorfer, A. Ebner, Nanotechnology 25 (2014).","mla":"Lamprecht, Constanze, et al. “A Single-Molecule Approach to Explore Binding Uptake and Transport of Cancer Cell Targeting Nanotubes.” Nanotechnology, vol. 25, no. 12, 125704, IOP Publishing, 2014, doi:10.1088/0957-4484/25/12/125704."},"publication":"Nanotechnology","article_type":"original","date_published":"2014-03-28T00:00:00Z","type":"journal_article","issue":"12","abstract":[{"lang":"eng","text":"In the past decade carbon nanotubes (CNTs) have been widely studied as a potential drug-delivery system, especially with functionality for cellular targeting. Yet, little is known about the actual process of docking to cell receptors and transport dynamics after internalization. Here we performed single-particle studies of folic acid (FA) mediated CNT binding to human carcinoma cells and their transport inside the cytosol. In particular, we employed molecular recognition force spectroscopy, an atomic force microscopy based method, to visualize and quantify docking of FA functionalized CNTs to FA binding receptors in terms of binding probability and binding force. We then traced individual fluorescently labeled, FA functionalized CNTs after specific uptake, and created a dynamic 'roadmap' that clearly showed trajectories of directed diffusion and areas of nanotube confinement in the cytosol. Our results demonstrate the potential of a single-molecule approach for investigation of drug-delivery vehicles and their targeting capacity."}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"1925","intvolume":" 25","ddc":["570"],"status":"public","title":"A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes","oa_version":"Submitted Version","file":[{"access_level":"open_access","file_name":"2014_Nanotechnology_Lamprecht.pdf","content_type":"application/pdf","file_size":3804152,"creator":"dernst","relation":"main_file","file_id":"7856","checksum":"df4e03d225a19179e7790f6d87a12332","date_created":"2020-05-15T09:21:19Z","date_updated":"2020-07-14T12:45:21Z"}],"month":"03","oa":1,"doi":"10.1088/0957-4484/25/12/125704","language":[{"iso":"eng"}],"article_number":"125704","publist_id":"5169","file_date_updated":"2020-07-14T12:45:21Z","acknowledgement":"This work was supported by EC grant Marie Curie RTN-CT-2006-035616, CARBIO 'Carbon nanotubes for biomedical applications' and Austrian FFG grant mnt-era.net 823980, 'IntelliTip'.\r\n","year":"2014","department":[{"_id":"CaHe"},{"_id":"MiSi"}],"publisher":"IOP Publishing","publication_status":"published","author":[{"first_name":"Constanze","last_name":"Lamprecht","full_name":"Lamprecht, Constanze"},{"full_name":"Plochberger, Birgit","first_name":"Birgit","last_name":"Plochberger"},{"id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4088-8633","first_name":"Verena","last_name":"Ruprecht","full_name":"Ruprecht, Verena"},{"full_name":"Wieser, Stefan","last_name":"Wieser","first_name":"Stefan","orcid":"0000-0002-2670-2217","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Rankl, Christian","first_name":"Christian","last_name":"Rankl"},{"first_name":"Elena","last_name":"Heister","full_name":"Heister, Elena"},{"full_name":"Unterauer, Barbara","last_name":"Unterauer","first_name":"Barbara"},{"first_name":"Mario","last_name":"Brameshuber","full_name":"Brameshuber, Mario"},{"full_name":"Danzberger, Jürgen","first_name":"Jürgen","last_name":"Danzberger"},{"full_name":"Lukanov, Petar","first_name":"Petar","last_name":"Lukanov"},{"last_name":"Flahaut","first_name":"Emmanuel","full_name":"Flahaut, Emmanuel"},{"last_name":"Schütz","first_name":"Gerhard","full_name":"Schütz, Gerhard"},{"full_name":"Hinterdorfer, Peter","last_name":"Hinterdorfer","first_name":"Peter"},{"last_name":"Ebner","first_name":"Andreas","full_name":"Ebner, Andreas"}],"volume":25,"date_created":"2018-12-11T11:54:45Z","date_updated":"2021-01-12T06:54:07Z"},{"intvolume":" 16","status":"public","title":"Active elastic thin shell theory for cellular deformations","ddc":["570"],"user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","_id":"1923","file":[{"creator":"system","content_type":"application/pdf","file_size":941387,"file_name":"IST-2016-429-v1+1_document.pdf","access_level":"open_access","date_created":"2018-12-12T10:16:16Z","date_updated":"2020-07-14T12:45:21Z","checksum":"8dbe81ec656bf1264d8889bda9b2b985","file_id":"5202","relation":"main_file"}],"oa_version":"Published Version","pubrep_id":"429","type":"journal_article","abstract":[{"text":"We derive the equations for a thin, axisymmetric elastic shell subjected to an internal active stress giving rise to active tension and moments within the shell. We discuss the stability of a cylindrical elastic shell and its response to a localized change in internal active stress. This description is relevant to describe the cellular actomyosin cortex, a thin shell at the cell surface behaving elastically at a short timescale and subjected to active internal forces arising from myosin molecular motor activity. We show that the recent observations of cell deformation following detachment of adherent cells (Maître J-L et al 2012 Science 338 253-6) are well accounted for by this mechanical description. The actin cortex elastic and bending moduli can be obtained from a quantitative analysis of cell shapes observed in these experiments. Our approach thus provides a non-invasive, imaging-based method for the extraction of cellular physical parameters.","lang":"eng"}],"citation":{"ama":"Berthoumieux H, Maître J-L, Heisenberg C-PJ, Paluch E, Julicher F, Salbreux G. Active elastic thin shell theory for cellular deformations. New Journal of Physics. 2014;16. doi:10.1088/1367-2630/16/6/065005","ieee":"H. Berthoumieux, J.-L. Maître, C.-P. J. Heisenberg, E. Paluch, F. Julicher, and G. Salbreux, “Active elastic thin shell theory for cellular deformations,” New Journal of Physics, vol. 16. IOP Publishing Ltd., 2014.","apa":"Berthoumieux, H., Maître, J.-L., Heisenberg, C.-P. J., Paluch, E., Julicher, F., & Salbreux, G. (2014). Active elastic thin shell theory for cellular deformations. New Journal of Physics. IOP Publishing Ltd. https://doi.org/10.1088/1367-2630/16/6/065005","ista":"Berthoumieux H, Maître J-L, Heisenberg C-PJ, Paluch E, Julicher F, Salbreux G. 2014. Active elastic thin shell theory for cellular deformations. New Journal of Physics. 16, 065005.","short":"H. Berthoumieux, J.-L. Maître, C.-P.J. Heisenberg, E. Paluch, F. Julicher, G. Salbreux, New Journal of Physics 16 (2014).","mla":"Berthoumieux, Hélène, et al. “Active Elastic Thin Shell Theory for Cellular Deformations.” New Journal of Physics, vol. 16, 065005, IOP Publishing Ltd., 2014, doi:10.1088/1367-2630/16/6/065005.","chicago":"Berthoumieux, Hélène, Jean-Léon Maître, Carl-Philipp J Heisenberg, Ewa Paluch, Frank Julicher, and Guillaume Salbreux. “Active Elastic Thin Shell Theory for Cellular Deformations.” New Journal of Physics. IOP Publishing Ltd., 2014. https://doi.org/10.1088/1367-2630/16/6/065005."},"publication":"New Journal of Physics","date_published":"2014-06-01T00:00:00Z","scopus_import":1,"has_accepted_license":"1","day":"01","publisher":"IOP Publishing Ltd.","department":[{"_id":"CaHe"}],"publication_status":"published","year":"2014","volume":16,"date_updated":"2021-01-12T06:54:06Z","date_created":"2018-12-11T11:54:44Z","author":[{"last_name":"Berthoumieux","first_name":"Hélène","full_name":"Berthoumieux, Hélène"},{"full_name":"Maître, Jean-Léon","last_name":"Maître","first_name":"Jean-Léon","orcid":"0000-0002-3688-1474","id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J"},{"first_name":"Ewa","last_name":"Paluch","full_name":"Paluch, Ewa"},{"last_name":"Julicher","first_name":"Frank","full_name":"Julicher, Frank"},{"full_name":"Salbreux, Guillaume","last_name":"Salbreux","first_name":"Guillaume"}],"article_number":"065005","publist_id":"5171","file_date_updated":"2020-07-14T12:45:21Z","quality_controlled":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"language":[{"iso":"eng"}],"doi":"10.1088/1367-2630/16/6/065005","month":"06"},{"abstract":[{"text":"Avian forelimb digit homology remains one of the standard themes in comparative biology and EvoDevo research. In order to resolve the apparent contradictions between embryological and paleontological evidence a variety of hypotheses have been presented in recent years. The proposals range from excluding birds from the dinosaur clade, to assignments of homology by different criteria, or even assuming a hexadactyl tetrapod limb ground state. At present two approaches prevail: the frame shift hypothesis and the pyramid reduction hypothesis. While the former postulates a homeotic shift of digit identities, the latter argues for a gradual bilateral reduction of phalanges and digits. Here we present a new model that integrates elements from both hypotheses with the existing experimental and fossil evidence. We start from the main feature common to both earlier concepts, the initiating ontogenetic event: reduction and loss of the anterior-most digit. It is proposed that a concerted mechanism of molecular regulation and developmental mechanics is capable of shifting the boundaries of hoxD expression in embryonic forelimb buds as well as changing the digit phenotypes. Based on a distinction between positional (topological) and compositional (phenotypic) homology criteria, we argue that the identity of the avian digits is II, III, IV, despite a partially altered phenotype. Finally, we introduce an alternative digit reduction scheme that reconciles the current fossil evidence with the presented molecular-morphogenetic model. Our approach identifies specific experiments that allow to test whether gene expression can be shifted and digit phenotypes can be altered by induced digit loss or digit gain.","lang":"eng"}],"issue":"1","publist_id":"4701","type":"journal_article","author":[{"full_name":"Capek, Daniel","id":"31C42484-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5199-9940","first_name":"Daniel","last_name":"Capek"},{"first_name":"Brian","last_name":"Metscher","full_name":"Metscher, Brian"},{"last_name":"Müller","first_name":"Gerd","full_name":"Müller, Gerd"}],"date_created":"2018-12-11T11:56:33Z","date_updated":"2021-01-12T06:56:16Z","oa_version":"None","volume":322,"_id":"2248","year":"2014","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","title":"Thumbs down: A molecular-morphogenetic approach to avian digit homology","status":"public","publication_status":"published","intvolume":" 322","publisher":"Wiley-Blackwell","department":[{"_id":"CaHe"}],"day":"01","month":"01","publication_identifier":{"issn":["15525007"]},"scopus_import":1,"date_published":"2014-01-01T00:00:00Z","doi":"10.1002/jez.b.22545","language":[{"iso":"eng"}],"publication":"Journal of Experimental Zoology Part B: Molecular and Developmental Evolution","citation":{"ama":"Capek D, Metscher B, Müller G. Thumbs down: A molecular-morphogenetic approach to avian digit homology. Journal of Experimental Zoology Part B: Molecular and Developmental Evolution. 2014;322(1):1-12. doi:10.1002/jez.b.22545","apa":"Capek, D., Metscher, B., & Müller, G. (2014). Thumbs down: A molecular-morphogenetic approach to avian digit homology. Journal of Experimental Zoology Part B: Molecular and Developmental Evolution. Wiley-Blackwell. https://doi.org/10.1002/jez.b.22545","ieee":"D. Capek, B. Metscher, and G. Müller, “Thumbs down: A molecular-morphogenetic approach to avian digit homology,” Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, vol. 322, no. 1. Wiley-Blackwell, pp. 1–12, 2014.","ista":"Capek D, Metscher B, Müller G. 2014. Thumbs down: A molecular-morphogenetic approach to avian digit homology. Journal of Experimental Zoology Part B: Molecular and Developmental Evolution. 322(1), 1–12.","short":"D. Capek, B. Metscher, G. Müller, Journal of Experimental Zoology Part B: Molecular and Developmental Evolution 322 (2014) 1–12.","mla":"Capek, Daniel, et al. “Thumbs down: A Molecular-Morphogenetic Approach to Avian Digit Homology.” Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, vol. 322, no. 1, Wiley-Blackwell, 2014, pp. 1–12, doi:10.1002/jez.b.22545.","chicago":"Capek, Daniel, Brian Metscher, and Gerd Müller. “Thumbs down: A Molecular-Morphogenetic Approach to Avian Digit Homology.” Journal of Experimental Zoology Part B: Molecular and Developmental Evolution. Wiley-Blackwell, 2014. https://doi.org/10.1002/jez.b.22545."},"quality_controlled":"1","page":"1 - 12"}]