--- _id: '12198' abstract: - lang: eng text: The Arabidopsis thaliana central cell, the companion cell of the egg, undergoes DNA demethylation before fertilization, but the targeting preferences, mechanism, and biological significance of this process remain unclear. Here, we show that active DNA demethylation mediated by the DEMETER DNA glycosylase accounts for all of the demethylation in the central cell and preferentially targets small, AT-rich, and nucleosome-depleted euchromatic transposable elements. The vegetative cell, the companion cell of sperm, also undergoes DEMETER-dependent demethylation of similar sequences, and lack of DEMETER in vegetative cells causes reduced small RNA–directed DNA methylation of transposons in sperm. Our results demonstrate that demethylation in companion cells reinforces transposon methylation in plant gametes and likely contributes to stable silencing of transposable elements across generations. acknowledgement: We thank S. Harmer for assistance with the analysis of histone modifications, the BioOptics team at the Vienna Biocenter Campus for sorting sperm and vegetative cell nuclei, K. Slotkin for the LAT52p-amiRNA=GFP plasmid, and G. Drews for the DD45p-GFP transgenic line. This work was partially funded by an NIH grant (GM69415) to R.L.F., NSF grants (MCB-0918821 and IOS-1025890) to R.L.F. and D.Z., a Young Investigator Grant from the Arnold and Mabel Beckman Foundation to D.Z., an Austrian Science Fund (FWF) grant P21389-B03 to H.T., a Ruth L. Kirschstein NIH Predoctoral Fellowship (GM093633) to C.A.I., a Fulbright Scholarship to J.A.R., a fellowship from the Jane Coffin Childs Memorial Fund to A.Z., and a Robert and Colleen Haas Scholarship to D.R. Sequencing data are deposited in GEO (GSE38935). article_processing_charge: No article_type: original author: - first_name: Christian A. full_name: Ibarra, Christian A. last_name: Ibarra - first_name: Xiaoqi full_name: Feng, Xiaoqi id: e0164712-22ee-11ed-b12a-d80fcdf35958 last_name: Feng orcid: 0000-0002-4008-1234 - first_name: Vera K. full_name: Schoft, Vera K. last_name: Schoft - first_name: Tzung-Fu full_name: Hsieh, Tzung-Fu last_name: Hsieh - first_name: Rie full_name: Uzawa, Rie last_name: Uzawa - first_name: Jessica A. full_name: Rodrigues, Jessica A. last_name: Rodrigues - first_name: Assaf full_name: Zemach, Assaf last_name: Zemach - first_name: Nina full_name: Chumak, Nina last_name: Chumak - first_name: Adriana full_name: Machlicova, Adriana last_name: Machlicova - first_name: Toshiro full_name: Nishimura, Toshiro last_name: Nishimura - first_name: Denisse full_name: Rojas, Denisse last_name: Rojas - first_name: Robert L. full_name: Fischer, Robert L. last_name: Fischer - first_name: Hisashi full_name: Tamaru, Hisashi last_name: Tamaru - first_name: Daniel full_name: Zilberman, Daniel last_name: Zilberman citation: ama: Ibarra CA, Feng X, Schoft VK, et al. Active DNA demethylation in plant companion cells reinforces transposon methylation in gametes. Science. 2012;337(6100):1360-1364. doi:10.1126/science.1224839 apa: Ibarra, C. A., Feng, X., Schoft, V. K., Hsieh, T.-F., Uzawa, R., Rodrigues, J. A., … Zilberman, D. (2012). Active DNA demethylation in plant companion cells reinforces transposon methylation in gametes. Science. American Association for the Advancement of Science. https://doi.org/10.1126/science.1224839 chicago: Ibarra, Christian A., Xiaoqi Feng, Vera K. Schoft, Tzung-Fu Hsieh, Rie Uzawa, Jessica A. Rodrigues, Assaf Zemach, et al. “Active DNA Demethylation in Plant Companion Cells Reinforces Transposon Methylation in Gametes.” Science. American Association for the Advancement of Science, 2012. https://doi.org/10.1126/science.1224839. ieee: C. A. Ibarra et al., “Active DNA demethylation in plant companion cells reinforces transposon methylation in gametes,” Science, vol. 337, no. 6100. American Association for the Advancement of Science, pp. 1360–1364, 2012. ista: Ibarra CA, Feng X, Schoft VK, Hsieh T-F, Uzawa R, Rodrigues JA, Zemach A, Chumak N, Machlicova A, Nishimura T, Rojas D, Fischer RL, Tamaru H, Zilberman D. 2012. Active DNA demethylation in plant companion cells reinforces transposon methylation in gametes. Science. 337(6100), 1360–1364. mla: Ibarra, Christian A., et al. “Active DNA Demethylation in Plant Companion Cells Reinforces Transposon Methylation in Gametes.” Science, vol. 337, no. 6100, American Association for the Advancement of Science, 2012, pp. 1360–64, doi:10.1126/science.1224839. short: C.A. Ibarra, X. Feng, V.K. Schoft, T.-F. Hsieh, R. Uzawa, J.A. Rodrigues, A. Zemach, N. Chumak, A. Machlicova, T. Nishimura, D. Rojas, R.L. Fischer, H. Tamaru, D. Zilberman, Science 337 (2012) 1360–1364. date_created: 2023-01-16T09:21:24Z date_published: 2012-09-14T00:00:00Z date_updated: 2023-10-16T09:27:26Z day: '14' department: - _id: XiFe doi: 10.1126/science.1224839 external_id: pmid: - '22984074' intvolume: ' 337' issue: '6100' keyword: - Multidisciplinary language: - iso: eng main_file_link: - open_access: '1' url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4034762/ month: '09' oa: 1 oa_version: Published Version page: 1360-1364 pmid: 1 publication: Science publication_identifier: eissn: - 1095-9203 issn: - 0036-8075 publication_status: published publisher: American Association for the Advancement of Science quality_controlled: '1' scopus_import: '1' status: public title: Active DNA demethylation in plant companion cells reinforces transposon methylation in gametes type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 337 year: '2012' ... --- _id: '6588' abstract: - lang: eng text: First we note that the best polynomial approximation to vertical bar x vertical bar on the set, which consists of an interval on the positive half-axis and a point on the negative half-axis, can be given by means of the classical Chebyshev polynomials. Then we explore the cases when a solution of the related problem on two intervals can be given in elementary functions. acknowledgement: "This work is supported by the Austrian Science Fund (FWF), Project P22025-N18.\r\n" article_processing_charge: No article_type: original author: - first_name: Florian full_name: Pausinger, Florian id: 2A77D7A2-F248-11E8-B48F-1D18A9856A87 last_name: Pausinger orcid: 0000-0002-8379-3768 citation: ama: Pausinger F. Elementary solutions of the Bernstein problem on two intervals. Journal of Mathematical Physics, Analysis, Geometry. 2012;8(1):63-78. apa: Pausinger, F. (2012). Elementary solutions of the Bernstein problem on two intervals. Journal of Mathematical Physics, Analysis, Geometry. B. Verkin Institute for Low Temperature Physics and Engineering. chicago: Pausinger, Florian. “Elementary Solutions of the Bernstein Problem on Two Intervals.” Journal of Mathematical Physics, Analysis, Geometry. B. Verkin Institute for Low Temperature Physics and Engineering, 2012. ieee: F. Pausinger, “Elementary solutions of the Bernstein problem on two intervals,” Journal of Mathematical Physics, Analysis, Geometry, vol. 8, no. 1. B. Verkin Institute for Low Temperature Physics and Engineering, pp. 63–78, 2012. ista: Pausinger F. 2012. Elementary solutions of the Bernstein problem on two intervals. Journal of Mathematical Physics, Analysis, Geometry. 8(1), 63–78. mla: Pausinger, Florian. “Elementary Solutions of the Bernstein Problem on Two Intervals.” Journal of Mathematical Physics, Analysis, Geometry, vol. 8, no. 1, B. Verkin Institute for Low Temperature Physics and Engineering, 2012, pp. 63–78. short: F. Pausinger, Journal of Mathematical Physics, Analysis, Geometry 8 (2012) 63–78. date_created: 2019-06-27T08:16:56Z date_published: 2012-01-01T00:00:00Z date_updated: 2023-10-16T09:41:31Z day: '01' department: - _id: HeEd external_id: isi: - '000301173600004' intvolume: ' 8' isi: 1 issue: '1' language: - iso: eng main_file_link: - open_access: '1' url: http://mi.mathnet.ru/eng/jmag525 month: '01' oa: 1 oa_version: Published Version page: 63-78 publication: Journal of Mathematical Physics, Analysis, Geometry publication_identifier: issn: - 1812-9471 publication_status: published publisher: B. Verkin Institute for Low Temperature Physics and Engineering quality_controlled: '1' scopus_import: '1' status: public title: Elementary solutions of the Bernstein problem on two intervals type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 8 year: '2012' ... --- _id: '3123' abstract: - lang: eng text: We introduce the idea of using an explicit triangle mesh to track the air/fluid interface in a smoothed particle hydrodynamics (SPH) simulator. Once an initial surface mesh is created, this mesh is carried forward in time using nearby particle velocities to advect the mesh vertices. The mesh connectivity remains mostly unchanged across time-steps; it is only modified locally for topology change events or for the improvement of triangle quality. In order to ensure that the surface mesh does not diverge from the underlying particle simulation, we periodically project the mesh surface onto an implicit surface defined by the physics simulation. The mesh surface gives us several advantages over previous SPH surface tracking techniques. We demonstrate a new method for surface tension calculations that clearly outperforms the state of the art in SPH surface tension for computer graphics. We also demonstrate a method for tracking detailed surface information (like colors) that is less susceptible to numerical diffusion than competing techniques. Finally, our temporally-coherent surface mesh allows us to simulate high-resolution surface wave dynamics without being limited by the particle resolution of the SPH simulation. acknowledgement: This work was funded by NSF grant IIS-1017014 and CCF- 0917093. alternative_title: - Eurographics article_processing_charge: No author: - first_name: Jihun full_name: Yu, Jihun last_name: Yu - first_name: Christopher J full_name: Wojtan, Christopher J id: 3C61F1D2-F248-11E8-B48F-1D18A9856A87 last_name: Wojtan orcid: 0000-0001-6646-5546 - first_name: Greg full_name: Turk, Greg last_name: Turk - first_name: Chee full_name: Yap, Chee last_name: Yap citation: ama: 'Yu J, Wojtan C, Turk G, Yap C. Explicit mesh surfaces for particle based fluids. In: Computer Graphics Forum. Vol 31. Wiley; 2012:815-824. doi:10.1111/j.1467-8659.2012.03062.x' apa: 'Yu, J., Wojtan, C., Turk, G., & Yap, C. (2012). Explicit mesh surfaces for particle based fluids. In Computer Graphics Forum (Vol. 31, pp. 815–824). Cagliari, Sardinia, Italy: Wiley. https://doi.org/10.1111/j.1467-8659.2012.03062.x' chicago: Yu, Jihun, Chris Wojtan, Greg Turk, and Chee Yap. “Explicit Mesh Surfaces for Particle Based Fluids.” In Computer Graphics Forum, 31:815–24. Wiley, 2012. https://doi.org/10.1111/j.1467-8659.2012.03062.x. ieee: J. Yu, C. Wojtan, G. Turk, and C. Yap, “Explicit mesh surfaces for particle based fluids,” in Computer Graphics Forum, Cagliari, Sardinia, Italy, 2012, vol. 31, no. 2, pp. 815–824. ista: 'Yu J, Wojtan C, Turk G, Yap C. 2012. Explicit mesh surfaces for particle based fluids. Computer Graphics Forum. EUROGRAPHICS: Conference on European Association for Computer Graphics, Eurographics, vol. 31, 815–824.' mla: Yu, Jihun, et al. “Explicit Mesh Surfaces for Particle Based Fluids.” Computer Graphics Forum, vol. 31, no. 2, Wiley, 2012, pp. 815–24, doi:10.1111/j.1467-8659.2012.03062.x. short: J. Yu, C. Wojtan, G. Turk, C. Yap, in:, Computer Graphics Forum, Wiley, 2012, pp. 815–824. conference: end_date: 2012-05-18 location: Cagliari, Sardinia, Italy name: 'EUROGRAPHICS: Conference on European Association for Computer Graphics' start_date: 2012-05-13 date_created: 2018-12-11T12:01:31Z date_published: 2012-05-01T00:00:00Z date_updated: 2023-10-16T09:54:40Z day: '01' ddc: - '000' department: - _id: ChWo doi: 10.1111/j.1467-8659.2012.03062.x file: - access_level: open_access checksum: acb325dd1e31859bedd30e013f61d0b9 content_type: application/pdf creator: system date_created: 2018-12-12T10:14:39Z date_updated: 2020-07-14T12:46:00Z file_id: '5092' file_name: IST-2016-601-v1+1_meshSPH.pdf file_size: 5740527 relation: main_file file_date_updated: 2020-07-14T12:46:00Z has_accepted_license: '1' intvolume: ' 31' issue: '2' language: - iso: eng month: '05' oa: 1 oa_version: Submitted Version page: 815 - 824 publication: Computer Graphics Forum publication_identifier: eissn: - 1467-8659 issn: - 0167-7055 publication_status: published publisher: Wiley publist_id: '3576' pubrep_id: '601' quality_controlled: '1' scopus_import: '1' status: public title: Explicit mesh surfaces for particle based fluids type: conference user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 31 year: '2012' ... --- _id: '3244' abstract: - lang: eng text: "Bibliothekare haben die Aufgabe, sich mit neuen Medienformen auseinanderzusetzen.\r\n" article_processing_charge: No article_type: letter_note author: - first_name: Patrick full_name: Danowski, Patrick id: 2EBD1598-F248-11E8-B48F-1D18A9856A87 last_name: Danowski orcid: 0000-0002-6026-4409 citation: ama: Danowski P. Die Zeit des Abwartens ist vorbei! BuB - Forum Bibliothek und Information. 2012;64(4):284. apa: Danowski, P. (2012). Die Zeit des Abwartens ist vorbei! BuB - Forum Bibliothek und Information. Fachzeitschrift des BIB – Berufsverband Information Bibliothek. chicago: Danowski, Patrick. “Die Zeit des Abwartens ist vorbei!” BuB - Forum Bibliothek und Information. Fachzeitschrift des BIB – Berufsverband Information Bibliothek, 2012. ieee: P. Danowski, “Die Zeit des Abwartens ist vorbei!,” BuB - Forum Bibliothek und Information, vol. 64, no. 4. Fachzeitschrift des BIB – Berufsverband Information Bibliothek, p. 284, 2012. ista: Danowski P. 2012. Die Zeit des Abwartens ist vorbei! BuB - Forum Bibliothek und Information. 64(4), 284. mla: Danowski, Patrick. “Die Zeit des Abwartens ist vorbei!” BuB - Forum Bibliothek und Information, vol. 64, no. 4, Fachzeitschrift des BIB – Berufsverband Information Bibliothek, 2012, p. 284. short: P. Danowski, BuB - Forum Bibliothek und Information 64 (2012) 284. date_created: 2018-12-11T12:02:13Z date_published: 2012-04-15T00:00:00Z date_updated: 2023-10-16T10:19:14Z day: '15' department: - _id: E-Lib intvolume: ' 64' issue: '4' language: - iso: ger main_file_link: - open_access: '1' url: https://www.b-u-b.de/fileadmin/archiv/imports/pdf_files/2012/bub_2012_04_284.pdf month: '04' oa: 1 oa_version: Published Version page: '284' popular_science: '1' publication: BuB - Forum Bibliothek und Information publication_identifier: issn: - 1869 -1137 publication_status: published publisher: Fachzeitschrift des BIB – Berufsverband Information Bibliothek publist_id: '3432' status: public title: Die Zeit des Abwartens ist vorbei! type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 64 year: '2012' ... --- _id: '3243' abstract: - lang: eng text: 'Wie wandelt sich das Berufsbild in Wissenschaftlichen Bibliotheken? Patrick Danowski gibt seine Einschätzung ab. ' article_processing_charge: No article_type: letter_note author: - first_name: Patrick full_name: Danowski, Patrick id: 2EBD1598-F248-11E8-B48F-1D18A9856A87 last_name: Danowski orcid: 0000-0002-6026-4409 citation: ama: Danowski P. Zwischen Technologie und Information. Büchereiperspektiven. 2012;2012(1):11. apa: Danowski, P. (2012). Zwischen Technologie und Information. Büchereiperspektiven. Büchereiverband Österreichs. chicago: Danowski, Patrick. “Zwischen Technologie und Information.” Büchereiperspektiven. Büchereiverband Österreichs, 2012. ieee: P. Danowski, “Zwischen Technologie und Information,” Büchereiperspektiven, vol. 2012, no. 1. Büchereiverband Österreichs, p. 11, 2012. ista: Danowski P. 2012. Zwischen Technologie und Information. Büchereiperspektiven. 2012(1), 11. mla: Danowski, Patrick. “Zwischen Technologie und Information.” Büchereiperspektiven, vol. 2012, no. 1, Büchereiverband Österreichs, 2012, p. 11. short: P. Danowski, Büchereiperspektiven 2012 (2012) 11. date_created: 2018-12-11T12:02:13Z date_published: 2012-03-01T00:00:00Z date_updated: 2023-10-16T10:40:18Z day: '01' department: - _id: E-Lib intvolume: ' 2012' issue: '1' language: - iso: ger main_file_link: - open_access: '1' url: https://www.bvoe.at/sites/default/files/2022-07/BP_1_12.pdf month: '03' oa: 1 oa_version: Published Version page: '11' popular_science: '1' publication: Büchereiperspektiven publication_identifier: issn: - 1607-7172 publication_status: published publisher: Büchereiverband Österreichs publist_id: '3433' status: public title: Zwischen Technologie und Information type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 2012 year: '2012' ...