---
_id: '1791'
abstract:
- lang: eng
text: Acute gene inactivation using short hairpin RNA (shRNA, knockdown) in developing
brain is a powerful technique to study genetic function; however, discrepancies
between knockdown and knockout murine phenotypes have left unanswered questions.
For example, doublecortin (Dcx) knockdown but not knockout shows a neocortical
neuronal migration phenotype. Here we report that in utero electroporation of
shRNA, but not siRNA or miRNA, to Dcx demonstrates a migration phenotype in Dcx
knockouts akin to the effect in wild-type mice, suggestingshRNA-mediated off-target
toxicity. This effect wasnot limited to Dcx, as it was observed in Dclk1 knockouts,
as well as with a fraction of scrambled shRNAs, suggesting a sequence-dependent
but not sequence-specific effect. Profiling RNAs from electroporated cells showed
a defect in endogenous let7 miRNA levels, and disruption of let7 or Dicer recapitulated
the migration defect. The results suggest that shRNA-mediated knockdown can produce
untoward migration effects by altering endogenous miRNA pathways.
acknowledgement: This work was supported by the National Institutes of Health R01NS41537.
G.K. was supported by an EMBO Long Term Fellowship, S.L.B. by the A.P. Giannini
Fellowship, and A.G.F. by the Brain Behavior Research Foundation
author:
- first_name: Seungtae
full_name: Baek, SeungTae
last_name: Baek
- first_name: Géraldine
full_name: Kerjan, Géraldine
last_name: Kerjan
- first_name: Stephanie
full_name: Bielas, Stephanie L
last_name: Bielas
- first_name: Jieun
full_name: Lee, Jieun
last_name: Lee
- first_name: Ali
full_name: Fenstermaker, Ali G
last_name: Fenstermaker
- first_name: Gaia
full_name: Gaia Novarino
id: 3E57A680-F248-11E8-B48F-1D18A9856A87
last_name: Novarino
orcid: 0000-0002-7673-7178
- first_name: Joseph
full_name: Gleeson, Joseph G
last_name: Gleeson
citation:
ama: Baek S, Kerjan G, Bielas S, et al. Off-target effect of doublecortin family
shRNA on neuronal migration associated with endogenous MicroRNA dysregulation.
Neuron. 2014;82(6):1255-1262. doi:10.1016/j.neuron.2014.04.036
apa: Baek, S., Kerjan, G., Bielas, S., Lee, J., Fenstermaker, A., Novarino, G.,
& Gleeson, J. (2014). Off-target effect of doublecortin family shRNA on neuronal
migration associated with endogenous MicroRNA dysregulation. Neuron. Elsevier.
https://doi.org/10.1016/j.neuron.2014.04.036
chicago: Baek, Seungtae, Géraldine Kerjan, Stephanie Bielas, Jieun Lee, Ali Fenstermaker,
Gaia Novarino, and Joseph Gleeson. “Off-Target Effect of Doublecortin Family ShRNA
on Neuronal Migration Associated with Endogenous MicroRNA Dysregulation.” Neuron.
Elsevier, 2014. https://doi.org/10.1016/j.neuron.2014.04.036.
ieee: S. Baek et al., “Off-target effect of doublecortin family shRNA on
neuronal migration associated with endogenous MicroRNA dysregulation,” Neuron,
vol. 82, no. 6. Elsevier, pp. 1255–1262, 2014.
ista: Baek S, Kerjan G, Bielas S, Lee J, Fenstermaker A, Novarino G, Gleeson J.
2014. Off-target effect of doublecortin family shRNA on neuronal migration associated
with endogenous MicroRNA dysregulation. Neuron. 82(6), 1255–1262.
mla: Baek, Seungtae, et al. “Off-Target Effect of Doublecortin Family ShRNA on Neuronal
Migration Associated with Endogenous MicroRNA Dysregulation.” Neuron, vol.
82, no. 6, Elsevier, 2014, pp. 1255–62, doi:10.1016/j.neuron.2014.04.036.
short: S. Baek, G. Kerjan, S. Bielas, J. Lee, A. Fenstermaker, G. Novarino, J. Gleeson,
Neuron 82 (2014) 1255–1262.
date_created: 2018-12-11T11:54:01Z
date_published: 2014-06-18T00:00:00Z
date_updated: 2021-01-12T06:53:13Z
day: '18'
doi: 10.1016/j.neuron.2014.04.036
extern: 1
intvolume: ' 82'
issue: '6'
month: '06'
page: 1255 - 1262
publication: Neuron
publication_status: published
publisher: Elsevier
publist_id: '5322'
quality_controlled: 0
status: public
title: Off-target effect of doublecortin family shRNA on neuronal migration associated
with endogenous MicroRNA dysregulation
type: journal_article
volume: 82
year: '2014'
...
---
_id: '1806'
abstract:
- lang: eng
text: The generation of asymmetry, at both cellular and tissue level, is one of
the most essential capabilities of all eukaryotic organisms. It mediates basically
all multicellular development ranging from embryogenesis and de novo organ formation
till responses to various environmental stimuli. In plants, the awe-inspiring
number of such processes is regulated by phytohormone auxin and its directional,
cell-to-cell transport. The mediators of this transport, PIN auxin transporters,
are asymmetrically localized at the plasma membrane, and this polar localization
determines the directionality of intercellular auxin flow. Thus, auxin transport
contributes crucially to the generation of local auxin gradients or maxima, which
instruct given cell to change its developmental program. Here, we introduce and
discuss the molecular components and cellular mechanisms regulating the generation
and maintenance of cellular PIN polarity, as the general hallmarks of cell polarity
in plants.
author:
- first_name: Pawel
full_name: Baster, Pawel
id: 3028BD74-F248-11E8-B48F-1D18A9856A87
last_name: Baster
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Baster P, Friml J. Auxin on the road navigated by cellular PIN polarity. In:
Zažímalová E, Petrášek J, Benková E, eds. Auxin and Its Role in Plant Development.
Springer; 2014:143-170. doi:10.1007/978-3-7091-1526-8_8'
apa: Baster, P., & Friml, J. (2014). Auxin on the road navigated by cellular
PIN polarity. In E. Zažímalová, J. Petrášek, & E. Benková (Eds.), Auxin
and Its Role in Plant Development (pp. 143–170). Springer. https://doi.org/10.1007/978-3-7091-1526-8_8
chicago: Baster, Pawel, and Jiří Friml. “Auxin on the Road Navigated by Cellular
PIN Polarity.” In Auxin and Its Role in Plant Development, edited by Eva
Zažímalová, Jan Petrášek, and Eva Benková, 143–70. Springer, 2014. https://doi.org/10.1007/978-3-7091-1526-8_8.
ieee: P. Baster and J. Friml, “Auxin on the road navigated by cellular PIN polarity,”
in Auxin and Its Role in Plant Development, E. Zažímalová, J. Petrášek,
and E. Benková, Eds. Springer, 2014, pp. 143–170.
ista: 'Baster P, Friml J. 2014.Auxin on the road navigated by cellular PIN polarity.
In: Auxin and Its Role in Plant Development. , 143–170.'
mla: Baster, Pawel, and Jiří Friml. “Auxin on the Road Navigated by Cellular PIN
Polarity.” Auxin and Its Role in Plant Development, edited by Eva Zažímalová
et al., Springer, 2014, pp. 143–70, doi:10.1007/978-3-7091-1526-8_8.
short: P. Baster, J. Friml, in:, E. Zažímalová, J. Petrášek, E. Benková (Eds.),
Auxin and Its Role in Plant Development, Springer, 2014, pp. 143–170.
date_created: 2018-12-11T11:54:07Z
date_published: 2014-04-01T00:00:00Z
date_updated: 2021-01-12T06:53:19Z
day: '01'
department:
- _id: JiFr
doi: 10.1007/978-3-7091-1526-8_8
editor:
- first_name: Eva
full_name: Zažímalová, Eva
last_name: Zažímalová
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
language:
- iso: eng
month: '04'
oa_version: None
page: 143 - 170
publication: Auxin and Its Role in Plant Development
publication_status: published
publisher: Springer
publist_id: '5304'
quality_controlled: '1'
scopus_import: 1
status: public
title: Auxin on the road navigated by cellular PIN polarity
type: book_chapter
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
year: '2014'
...
---
_id: '1816'
abstract:
- lang: eng
text: Watermarking techniques for vector graphics dislocate vertices in order to
embed imperceptible, yet detectable, statistical features into the input data.
The embedding process may result in a change of the topology of the input data,
e.g., by introducing self-intersections, which is undesirable or even disastrous
for many applications. In this paper we present a watermarking framework for two-dimensional
vector graphics that employs conventional watermarking techniques but still provides
the guarantee that the topology of the input data is preserved. The geometric
part of this framework computes so-called maximum perturbation regions (MPR) of
vertices. We propose two efficient algorithms to compute MPRs based on Voronoi
diagrams and constrained triangulations. Furthermore, we present two algorithms
to conditionally correct the watermarked data in order to increase the watermark
embedding capacity and still guarantee topological correctness. While we focus
on the watermarking of input formed by straight-line segments, one of our approaches
can also be extended to circular arcs. We conclude the paper by demonstrating
and analyzing the applicability of our framework in conjunction with two well-known
watermarking techniques.
acknowledgement: 'Work by Martin Held and Stefan Huber was supported by Austrian Science
Fund (FWF): L367-N15 and P25816-N15.'
author:
- first_name: Stefan
full_name: Huber, Stefan
id: 4700A070-F248-11E8-B48F-1D18A9856A87
last_name: Huber
orcid: 0000-0002-8871-5814
- first_name: Martin
full_name: Held, Martin
last_name: Held
- first_name: Peter
full_name: Meerwald, Peter
last_name: Meerwald
- first_name: Roland
full_name: Kwitt, Roland
last_name: Kwitt
citation:
ama: Huber S, Held M, Meerwald P, Kwitt R. Topology-preserving watermarking of vector
graphics. International Journal of Computational Geometry and Applications.
2014;24(1):61-86. doi:10.1142/S0218195914500034
apa: Huber, S., Held, M., Meerwald, P., & Kwitt, R. (2014). Topology-preserving
watermarking of vector graphics. International Journal of Computational Geometry
and Applications. World Scientific Publishing. https://doi.org/10.1142/S0218195914500034
chicago: Huber, Stefan, Martin Held, Peter Meerwald, and Roland Kwitt. “Topology-Preserving
Watermarking of Vector Graphics.” International Journal of Computational Geometry
and Applications. World Scientific Publishing, 2014. https://doi.org/10.1142/S0218195914500034.
ieee: S. Huber, M. Held, P. Meerwald, and R. Kwitt, “Topology-preserving watermarking
of vector graphics,” International Journal of Computational Geometry and Applications,
vol. 24, no. 1. World Scientific Publishing, pp. 61–86, 2014.
ista: Huber S, Held M, Meerwald P, Kwitt R. 2014. Topology-preserving watermarking
of vector graphics. International Journal of Computational Geometry and Applications.
24(1), 61–86.
mla: Huber, Stefan, et al. “Topology-Preserving Watermarking of Vector Graphics.”
International Journal of Computational Geometry and Applications, vol.
24, no. 1, World Scientific Publishing, 2014, pp. 61–86, doi:10.1142/S0218195914500034.
short: S. Huber, M. Held, P. Meerwald, R. Kwitt, International Journal of Computational
Geometry and Applications 24 (2014) 61–86.
date_created: 2018-12-11T11:54:10Z
date_published: 2014-03-16T00:00:00Z
date_updated: 2021-01-12T06:53:23Z
day: '16'
ddc:
- '000'
department:
- _id: HeEd
doi: 10.1142/S0218195914500034
file:
- access_level: open_access
checksum: be45c133ab4d43351260e21beaa8f4b1
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:08:43Z
date_updated: 2020-07-14T12:45:17Z
file_id: '4704'
file_name: IST-2016-443-v1+1_S0218195914500034.pdf
file_size: 991734
relation: main_file
file_date_updated: 2020-07-14T12:45:17Z
has_accepted_license: '1'
intvolume: ' 24'
issue: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '03'
oa: 1
oa_version: Published Version
page: 61 - 86
publication: International Journal of Computational Geometry and Applications
publication_status: published
publisher: World Scientific Publishing
publist_id: '5290'
pubrep_id: '443'
quality_controlled: '1'
scopus_import: 1
status: public
title: Topology-preserving watermarking of vector graphics
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 24
year: '2014'
...
---
_id: '1821'
abstract:
- lang: eng
text: We review recent progress towards a rigorous understanding of the Bogoliubov
approximation for bosonic quantum many-body systems. We focus, in particular,
on the excitation spectrum of a Bose gas in the mean-field (Hartree) limit. A
list of open problems will be discussed at the end.
article_number: '1.4881536'
author:
- first_name: Robert
full_name: Seiringer, Robert
id: 4AFD0470-F248-11E8-B48F-1D18A9856A87
last_name: Seiringer
orcid: 0000-0002-6781-0521
citation:
ama: Seiringer R. Bose gases, Bose-Einstein condensation, and the Bogoliubov approximation.
Journal of Mathematical Physics. 2014;55(7). doi:10.1063/1.4881536
apa: Seiringer, R. (2014). Bose gases, Bose-Einstein condensation, and the Bogoliubov
approximation. Journal of Mathematical Physics. American Institute of Physics.
https://doi.org/10.1063/1.4881536
chicago: Seiringer, Robert. “Bose Gases, Bose-Einstein Condensation, and the Bogoliubov
Approximation.” Journal of Mathematical Physics. American Institute of
Physics, 2014. https://doi.org/10.1063/1.4881536.
ieee: R. Seiringer, “Bose gases, Bose-Einstein condensation, and the Bogoliubov
approximation,” Journal of Mathematical Physics, vol. 55, no. 7. American
Institute of Physics, 2014.
ista: Seiringer R. 2014. Bose gases, Bose-Einstein condensation, and the Bogoliubov
approximation. Journal of Mathematical Physics. 55(7), 1.4881536.
mla: Seiringer, Robert. “Bose Gases, Bose-Einstein Condensation, and the Bogoliubov
Approximation.” Journal of Mathematical Physics, vol. 55, no. 7, 1.4881536,
American Institute of Physics, 2014, doi:10.1063/1.4881536.
short: R. Seiringer, Journal of Mathematical Physics 55 (2014).
date_created: 2018-12-11T11:54:11Z
date_published: 2014-06-26T00:00:00Z
date_updated: 2021-01-12T06:53:25Z
day: '26'
ddc:
- '510'
- '530'
department:
- _id: RoSe
doi: 10.1063/1.4881536
file:
- access_level: open_access
checksum: ed0efc93c10f1341155f0316af617b82
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:15:49Z
date_updated: 2020-07-14T12:45:17Z
file_id: '5172'
file_name: IST-2016-532-v1+1_J._Mathematical_Phys._2014_Seiringer.pdf
file_size: 269171
relation: main_file
file_date_updated: 2020-07-14T12:45:17Z
has_accepted_license: '1'
intvolume: ' 55'
issue: '7'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Submitted Version
project:
- _id: 26450934-B435-11E9-9278-68D0E5697425
name: NSERC Postdoctoral fellowship
publication: Journal of Mathematical Physics
publication_status: published
publisher: American Institute of Physics
publist_id: '5285'
pubrep_id: '532'
quality_controlled: '1'
scopus_import: 1
status: public
title: Bose gases, Bose-Einstein condensation, and the Bogoliubov approximation
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 55
year: '2014'
...
---
_id: '1822'
article_number: '075101'
author:
- first_name: Vojkan
full_name: Jakšić, Vojkan
last_name: Jakšić
- first_name: Claude
full_name: Pillet, Claude
last_name: Pillet
- first_name: Robert
full_name: Seiringer, Robert
id: 4AFD0470-F248-11E8-B48F-1D18A9856A87
last_name: Seiringer
orcid: 0000-0002-6781-0521
citation:
ama: Jakšić V, Pillet C, Seiringer R. Introduction. Journal of Mathematical Physics.
2014;55(7). doi:10.1063/1.4884877
apa: Jakšić, V., Pillet, C., & Seiringer, R. (2014). Introduction. Journal
of Mathematical Physics. American Institute of Physics. https://doi.org/10.1063/1.4884877
chicago: Jakšić, Vojkan, Claude Pillet, and Robert Seiringer. “Introduction.” Journal
of Mathematical Physics. American Institute of Physics, 2014. https://doi.org/10.1063/1.4884877.
ieee: V. Jakšić, C. Pillet, and R. Seiringer, “Introduction,” Journal of Mathematical
Physics, vol. 55, no. 7. American Institute of Physics, 2014.
ista: Jakšić V, Pillet C, Seiringer R. 2014. Introduction. Journal of Mathematical
Physics. 55(7), 075101.
mla: Jakšić, Vojkan, et al. “Introduction.” Journal of Mathematical Physics,
vol. 55, no. 7, 075101, American Institute of Physics, 2014, doi:10.1063/1.4884877.
short: V. Jakšić, C. Pillet, R. Seiringer, Journal of Mathematical Physics 55 (2014).
date_created: 2018-12-11T11:54:12Z
date_published: 2014-07-01T00:00:00Z
date_updated: 2021-01-12T06:53:25Z
day: '01'
department:
- _id: RoSe
doi: 10.1063/1.4884877
intvolume: ' 55'
issue: '7'
language:
- iso: eng
month: '07'
oa_version: None
publication: Journal of Mathematical Physics
publication_status: published
publisher: American Institute of Physics
publist_id: '5284'
quality_controlled: '1'
scopus_import: 1
status: public
title: Introduction
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 55
year: '2014'
...