---
_id: '7476'
abstract:
- lang: eng
text: The sebaceous gland (SG) is an essential component of the skin, and SG dysfunction
is debilitating1,2. Yet, the cellular bases for its origin, development and subsequent
maintenance remain poorly understood. Here, we apply large-scale quantitative
fate mapping to define the patterns of cell fate behaviour during SG development
and maintenance. We show that the SG develops from a defined number of lineage-restricted
progenitors that undergo a programme of independent and stochastic cell fate decisions.
Following an expansion phase, equipotent progenitors transition into a phase of
homeostatic turnover, which is correlated with changes in the mechanical properties
of the stroma and spatial restrictions on gland size. Expression of the oncogene
KrasG12D results in a release from these constraints and unbridled gland expansion.
Quantitative clonal fate analysis reveals that, during this phase, the primary
effect of the Kras oncogene is to drive a constant fate bias with little effect
on cell division rates. These findings provide insight into the developmental
programme of the SG, as well as the mechanisms that drive tumour progression and
gland dysfunction.
article_processing_charge: No
article_type: original
author:
- first_name: Marianne Stemann
full_name: Andersen, Marianne Stemann
last_name: Andersen
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Svetlana
full_name: Ulyanchenko, Svetlana
last_name: Ulyanchenko
- first_name: Soline
full_name: Estrach, Soline
last_name: Estrach
- first_name: Yasuko
full_name: Antoku, Yasuko
last_name: Antoku
- first_name: Sabrina
full_name: Pisano, Sabrina
last_name: Pisano
- first_name: Kim E.
full_name: Boonekamp, Kim E.
last_name: Boonekamp
- first_name: Sarah
full_name: Sendrup, Sarah
last_name: Sendrup
- first_name: Martti
full_name: Maimets, Martti
last_name: Maimets
- first_name: Marianne Terndrup
full_name: Pedersen, Marianne Terndrup
last_name: Pedersen
- first_name: Jens V.
full_name: Johansen, Jens V.
last_name: Johansen
- first_name: Ditte L.
full_name: Clement, Ditte L.
last_name: Clement
- first_name: Chloe C.
full_name: Feral, Chloe C.
last_name: Feral
- first_name: Benjamin D.
full_name: Simons, Benjamin D.
last_name: Simons
- first_name: Kim B.
full_name: Jensen, Kim B.
last_name: Jensen
citation:
ama: Andersen MS, Hannezo EB, Ulyanchenko S, et al. Tracing the cellular dynamics
of sebaceous gland development in normal and perturbed states. Nature Cell
Biology. 2019;21(8):924-932. doi:10.1038/s41556-019-0362-x
apa: Andersen, M. S., Hannezo, E. B., Ulyanchenko, S., Estrach, S., Antoku, Y.,
Pisano, S., … Jensen, K. B. (2019). Tracing the cellular dynamics of sebaceous
gland development in normal and perturbed states. Nature Cell Biology.
Springer Nature. https://doi.org/10.1038/s41556-019-0362-x
chicago: Andersen, Marianne Stemann, Edouard B Hannezo, Svetlana Ulyanchenko, Soline
Estrach, Yasuko Antoku, Sabrina Pisano, Kim E. Boonekamp, et al. “Tracing the
Cellular Dynamics of Sebaceous Gland Development in Normal and Perturbed States.”
Nature Cell Biology. Springer Nature, 2019. https://doi.org/10.1038/s41556-019-0362-x.
ieee: M. S. Andersen et al., “Tracing the cellular dynamics of sebaceous
gland development in normal and perturbed states,” Nature Cell Biology,
vol. 21, no. 8. Springer Nature, pp. 924–932, 2019.
ista: Andersen MS, Hannezo EB, Ulyanchenko S, Estrach S, Antoku Y, Pisano S, Boonekamp
KE, Sendrup S, Maimets M, Pedersen MT, Johansen JV, Clement DL, Feral CC, Simons
BD, Jensen KB. 2019. Tracing the cellular dynamics of sebaceous gland development
in normal and perturbed states. Nature Cell Biology. 21(8), 924–932.
mla: Andersen, Marianne Stemann, et al. “Tracing the Cellular Dynamics of Sebaceous
Gland Development in Normal and Perturbed States.” Nature Cell Biology,
vol. 21, no. 8, Springer Nature, 2019, pp. 924–32, doi:10.1038/s41556-019-0362-x.
short: M.S. Andersen, E.B. Hannezo, S. Ulyanchenko, S. Estrach, Y. Antoku, S. Pisano,
K.E. Boonekamp, S. Sendrup, M. Maimets, M.T. Pedersen, J.V. Johansen, D.L. Clement,
C.C. Feral, B.D. Simons, K.B. Jensen, Nature Cell Biology 21 (2019) 924–932.
date_created: 2020-02-11T08:43:49Z
date_published: 2019-08-01T00:00:00Z
date_updated: 2021-01-12T08:13:47Z
day: '01'
doi: 10.1038/s41556-019-0362-x
extern: '1'
external_id:
pmid:
- '31358966'
intvolume: ' 21'
issue: '8'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978139/
month: '08'
oa: 1
oa_version: Submitted Version
page: 924-932
pmid: 1
publication: Nature Cell Biology
publication_identifier:
issn:
- 1465-7392
- 1476-4679
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
status: public
title: Tracing the cellular dynamics of sebaceous gland development in normal and
perturbed states
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 21
year: '2019'
...
---
_id: '7548'
abstract:
- lang: eng
text: Although the aggregation of the amyloid-β peptide (Aβ) into amyloid fibrils
is a well-established hallmark of Alzheimer’s disease, the complex mechanisms
linking this process to neurodegeneration are still incompletely understood. The
nematode worm C. elegans is a valuable model organism through which to study these
mechanisms because of its simple nervous system and its relatively short lifespan.
Standard Aβ-based C. elegans models of Alzheimer’s disease are designed to study
the toxic effects of the overexpression of Aβ in the muscle or nervous systems.
However, the wide variety of effects associated with the tissue-level overexpression
of Aβ makes it difficult to single out and study specific cellular mechanisms
related to the onset of Alzheimer’s disease. Here, to better understand how to
investigate the early events affecting neuronal signalling, we created a C. elegans
model expressing Aβ42, the 42-residue form of Aβ, from a single-copy gene insertion
in just one pair of glutamatergic sensory neurons, the BAG neurons. In behavioural
assays, we found that the Aβ42-expressing animals displayed a subtle modulation
of the response to CO2, compared to controls. Ca2+ imaging revealed that the BAG
neurons in young Aβ42-expressing nematodes were activated more strongly than in
control animals, and that neuronal activation remained intact until old age. Taken
together, our results suggest that Aβ42-expression in this very subtle model of
AD is sufficient to modulate the behavioural response but not strong enough to
generate significant neurotoxicity, suggesting that slightly more aggressive perturbations
will enable effectively studies of the links between the modulation of a physiological
response and its associated neurotoxicity.
article_number: e0217746
article_processing_charge: No
article_type: original
author:
- first_name: Tessa
full_name: Sinnige, Tessa
last_name: Sinnige
- first_name: Prashanth
full_name: Ciryam, Prashanth
last_name: Ciryam
- first_name: Samuel
full_name: Casford, Samuel
last_name: Casford
- first_name: Christopher M.
full_name: Dobson, Christopher M.
last_name: Dobson
- first_name: Mario
full_name: de Bono, Mario
id: 4E3FF80E-F248-11E8-B48F-1D18A9856A87
last_name: de Bono
orcid: 0000-0001-8347-0443
- first_name: Michele
full_name: Vendruscolo, Michele
last_name: Vendruscolo
citation:
ama: Sinnige T, Ciryam P, Casford S, Dobson CM, de Bono M, Vendruscolo M. Expression
of the amyloid-β peptide in a single pair of C. elegans sensory neurons modulates
the associated behavioural response. PLOS ONE. 2019;14(5). doi:10.1371/journal.pone.0217746
apa: Sinnige, T., Ciryam, P., Casford, S., Dobson, C. M., de Bono, M., & Vendruscolo,
M. (2019). Expression of the amyloid-β peptide in a single pair of C. elegans
sensory neurons modulates the associated behavioural response. PLOS ONE.
Public Library of Science. https://doi.org/10.1371/journal.pone.0217746
chicago: Sinnige, Tessa, Prashanth Ciryam, Samuel Casford, Christopher M. Dobson,
Mario de Bono, and Michele Vendruscolo. “Expression of the Amyloid-β Peptide in
a Single Pair of C. Elegans Sensory Neurons Modulates the Associated Behavioural
Response.” PLOS ONE. Public Library of Science, 2019. https://doi.org/10.1371/journal.pone.0217746.
ieee: T. Sinnige, P. Ciryam, S. Casford, C. M. Dobson, M. de Bono, and M. Vendruscolo,
“Expression of the amyloid-β peptide in a single pair of C. elegans sensory neurons
modulates the associated behavioural response,” PLOS ONE, vol. 14, no.
5. Public Library of Science, 2019.
ista: Sinnige T, Ciryam P, Casford S, Dobson CM, de Bono M, Vendruscolo M. 2019.
Expression of the amyloid-β peptide in a single pair of C. elegans sensory neurons
modulates the associated behavioural response. PLOS ONE. 14(5), e0217746.
mla: Sinnige, Tessa, et al. “Expression of the Amyloid-β Peptide in a Single Pair
of C. Elegans Sensory Neurons Modulates the Associated Behavioural Response.”
PLOS ONE, vol. 14, no. 5, e0217746, Public Library of Science, 2019, doi:10.1371/journal.pone.0217746.
short: T. Sinnige, P. Ciryam, S. Casford, C.M. Dobson, M. de Bono, M. Vendruscolo,
PLOS ONE 14 (2019).
date_created: 2020-02-28T10:45:13Z
date_published: 2019-05-31T00:00:00Z
date_updated: 2021-01-12T08:14:08Z
day: '31'
doi: 10.1371/journal.pone.0217746
extern: '1'
intvolume: ' 14'
issue: '5'
language:
- iso: eng
month: '05'
oa_version: Published Version
publication: PLOS ONE
publication_identifier:
issn:
- 1932-6203
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
status: public
title: Expression of the amyloid-β peptide in a single pair of C. elegans sensory
neurons modulates the associated behavioural response
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 14
year: '2019'
...
---
_id: '7547'
abstract:
- lang: eng
text: The BH3-only family of proteins is key for initiating apoptosis in a variety
of contexts, and may also contribute to non-apoptotic cellular processes. Historically,
the nematode Caenorhabditis elegans has provided a powerful system for studying
and identifying conserved regulators of BH3-only proteins. In C. elegans, the
BH3-only protein egl-1 is expressed during development to cell-autonomously trigger
most developmental cell deaths. Here we provide evidence that egl-1 is also transcribed
after development in the sensory neuron pair URX without inducing apoptosis. We
used genetic screening and epistasis analysis to determine that its transcription
is regulated in URX by neuronal activity and/or in parallel by orthologs of Protein
Kinase G and the Salt-Inducible Kinase family. Because several BH3-only family
proteins are also expressed in the adult nervous system of mammals, we suggest
that studying egl-1 expression in URX may shed light on mechanisms that regulate
conserved family members in higher organisms.
article_processing_charge: No
article_type: original
author:
- first_name: Jesse
full_name: Cohn, Jesse
last_name: Cohn
- first_name: Vivek
full_name: Dwivedi, Vivek
last_name: Dwivedi
- first_name: Giulio
full_name: Valperga, Giulio
last_name: Valperga
- first_name: Nicole
full_name: Zarate, Nicole
last_name: Zarate
- first_name: Mario
full_name: de Bono, Mario
id: 4E3FF80E-F248-11E8-B48F-1D18A9856A87
last_name: de Bono
orcid: 0000-0001-8347-0443
- first_name: H. Robert
full_name: Horvitz, H. Robert
last_name: Horvitz
- first_name: Jonathan T.
full_name: Pierce, Jonathan T.
last_name: Pierce
citation:
ama: 'Cohn J, Dwivedi V, Valperga G, et al. Activity-dependent regulation of the
proapoptotic BH3-only gene egl-1 in a living neuron pair in Caenorhabditis elegans.
G3: Genes, Genomes, Genetics. 2019;9(11):3703-3714. doi:10.1534/g3.119.400654'
apa: 'Cohn, J., Dwivedi, V., Valperga, G., Zarate, N., de Bono, M., Horvitz, H.
R., & Pierce, J. T. (2019). Activity-dependent regulation of the proapoptotic
BH3-only gene egl-1 in a living neuron pair in Caenorhabditis elegans. G3:
Genes, Genomes, Genetics. Genetics Society of America. https://doi.org/10.1534/g3.119.400654'
chicago: 'Cohn, Jesse, Vivek Dwivedi, Giulio Valperga, Nicole Zarate, Mario de Bono,
H. Robert Horvitz, and Jonathan T. Pierce. “Activity-Dependent Regulation of the
Proapoptotic BH3-Only Gene Egl-1 in a Living Neuron Pair in Caenorhabditis Elegans.”
G3: Genes, Genomes, Genetics. Genetics Society of America, 2019. https://doi.org/10.1534/g3.119.400654.'
ieee: 'J. Cohn et al., “Activity-dependent regulation of the proapoptotic
BH3-only gene egl-1 in a living neuron pair in Caenorhabditis elegans,” G3:
Genes, Genomes, Genetics, vol. 9, no. 11. Genetics Society of America, pp.
3703–3714, 2019.'
ista: 'Cohn J, Dwivedi V, Valperga G, Zarate N, de Bono M, Horvitz HR, Pierce JT.
2019. Activity-dependent regulation of the proapoptotic BH3-only gene egl-1 in
a living neuron pair in Caenorhabditis elegans. G3: Genes, Genomes, Genetics.
9(11), 3703–3714.'
mla: 'Cohn, Jesse, et al. “Activity-Dependent Regulation of the Proapoptotic BH3-Only
Gene Egl-1 in a Living Neuron Pair in Caenorhabditis Elegans.” G3: Genes, Genomes,
Genetics, vol. 9, no. 11, Genetics Society of America, 2019, pp. 3703–14,
doi:10.1534/g3.119.400654.'
short: 'J. Cohn, V. Dwivedi, G. Valperga, N. Zarate, M. de Bono, H.R. Horvitz, J.T.
Pierce, G3: Genes, Genomes, Genetics 9 (2019) 3703–3714.'
date_created: 2020-02-28T10:44:27Z
date_published: 2019-11-01T00:00:00Z
date_updated: 2021-01-12T08:14:07Z
day: '01'
doi: 10.1534/g3.119.400654
extern: '1'
external_id:
pmid:
- '31519744'
intvolume: ' 9'
issue: '11'
language:
- iso: eng
month: '11'
oa_version: Published Version
page: 3703-3714
pmid: 1
publication: 'G3: Genes, Genomes, Genetics'
publication_identifier:
issn:
- 2160-1836
publication_status: published
publisher: Genetics Society of America
quality_controlled: '1'
status: public
title: Activity-dependent regulation of the proapoptotic BH3-only gene egl-1 in a
living neuron pair in Caenorhabditis elegans
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 9
year: '2019'
...
---
_id: '7550'
abstract:
- lang: eng
text: 'We consider an optimal control problem for an abstract nonlinear dissipative
evolution equation. The differential constraint is penalized by augmenting the
target functional by a nonnegative global-in-time functional which is null-minimized
in the evolution equation is satisfied. Different variational settings are presented,
leading to the convergence of the penalization method for gradient flows, noncyclic
and semimonotone flows, doubly nonlinear evolutions, and GENERIC systems. '
acknowledgement: This work is supported by Vienna Science and Technology Fund (WWTF)
through Project MA14-009 and by the Austrian Science Fund (FWF) projects F 65 and
I 2375.
article_processing_charge: No
article_type: original
author:
- first_name: Lorenzo
full_name: Portinale, Lorenzo
id: 30AD2CBC-F248-11E8-B48F-1D18A9856A87
last_name: Portinale
- first_name: Ulisse
full_name: Stefanelli, Ulisse
last_name: Stefanelli
citation:
ama: Portinale L, Stefanelli U. Penalization via global functionals of optimal-control
problems for dissipative evolution. Advances in Mathematical Sciences and Applications.
2019;28(2):425-447.
apa: Portinale, L., & Stefanelli, U. (2019). Penalization via global functionals
of optimal-control problems for dissipative evolution. Advances in Mathematical
Sciences and Applications. Gakko Tosho.
chicago: Portinale, Lorenzo, and Ulisse Stefanelli. “Penalization via Global Functionals
of Optimal-Control Problems for Dissipative Evolution.” Advances in Mathematical
Sciences and Applications. Gakko Tosho, 2019.
ieee: L. Portinale and U. Stefanelli, “Penalization via global functionals of optimal-control
problems for dissipative evolution,” Advances in Mathematical Sciences and
Applications, vol. 28, no. 2. Gakko Tosho, pp. 425–447, 2019.
ista: Portinale L, Stefanelli U. 2019. Penalization via global functionals of optimal-control
problems for dissipative evolution. Advances in Mathematical Sciences and Applications.
28(2), 425–447.
mla: Portinale, Lorenzo, and Ulisse Stefanelli. “Penalization via Global Functionals
of Optimal-Control Problems for Dissipative Evolution.” Advances in Mathematical
Sciences and Applications, vol. 28, no. 2, Gakko Tosho, 2019, pp. 425–47.
short: L. Portinale, U. Stefanelli, Advances in Mathematical Sciences and Applications
28 (2019) 425–447.
date_created: 2020-02-28T10:54:41Z
date_published: 2019-10-22T00:00:00Z
date_updated: 2022-06-17T07:52:41Z
day: '22'
department:
- _id: JaMa
external_id:
arxiv:
- '1910.10050'
intvolume: ' 28'
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: ' https://doi.org/10.48550/arXiv.1910.10050'
month: '10'
oa: 1
oa_version: Preprint
page: 425-447
project:
- _id: fc31cba2-9c52-11eb-aca3-ff467d239cd2
grant_number: F6504
name: Taming Complexity in Partial Differential Systems
publication: Advances in Mathematical Sciences and Applications
publication_identifier:
issn:
- 1343-4373
publication_status: published
publisher: Gakko Tosho
quality_controlled: '1'
status: public
title: Penalization via global functionals of optimal-control problems for dissipative
evolution
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 28
year: '2019'
...
---
_id: '7552'
abstract:
- lang: eng
text: 'There is increasing evidence that protein binding to specific sites along
DNA can activate the reading out of genetic information without coming into direct
physical contact with the gene. There also is evidence that these distant but
interacting sites are embedded in a liquid droplet of proteins which condenses
out of the surrounding solution. We argue that droplet-mediated interactions can
account for crucial features of gene regulation only if the droplet is poised
at a non-generic point in its phase diagram. We explore a minimal model that embodies
this idea, show that this model has a natural mechanism for self-tuning, and suggest
direct experimental tests. '
article_processing_charge: No
author:
- first_name: William
full_name: Bialek, William
last_name: Bialek
- first_name: Thomas
full_name: Gregor, Thomas
last_name: Gregor
- first_name: Gašper
full_name: Tkačik, Gašper
id: 3D494DCA-F248-11E8-B48F-1D18A9856A87
last_name: Tkačik
orcid: 0000-0002-6699-1455
citation:
ama: Bialek W, Gregor T, Tkačik G. Action at a distance in transcriptional regulation.
arXiv:191208579.
apa: Bialek, W., Gregor, T., & Tkačik, G. (n.d.). Action at a distance in transcriptional
regulation. arXiv:1912.08579. ArXiv.
chicago: Bialek, William, Thomas Gregor, and Gašper Tkačik. “Action at a Distance
in Transcriptional Regulation.” ArXiv:1912.08579. ArXiv, n.d.
ieee: W. Bialek, T. Gregor, and G. Tkačik, “Action at a distance in transcriptional
regulation,” arXiv:1912.08579. ArXiv.
ista: Bialek W, Gregor T, Tkačik G. Action at a distance in transcriptional regulation.
arXiv:1912.08579, .
mla: Bialek, William, et al. “Action at a Distance in Transcriptional Regulation.”
ArXiv:1912.08579, ArXiv.
short: W. Bialek, T. Gregor, G. Tkačik, ArXiv:1912.08579 (n.d.).
date_created: 2020-02-28T10:57:08Z
date_published: 2019-12-18T00:00:00Z
date_updated: 2021-01-12T08:14:09Z
day: '18'
department:
- _id: GaTk
external_id:
arxiv:
- '1912.08579'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://arxiv.org/abs/1912.08579
month: '12'
oa: 1
oa_version: Preprint
page: '5'
project:
- _id: 254E9036-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P28844-B27
name: Biophysics of information processing in gene regulation
publication: arXiv:1912.08579
publication_status: submitted
publisher: ArXiv
status: public
title: Action at a distance in transcriptional regulation
type: preprint
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2019'
...