---
_id: '7888'
abstract:
- lang: eng
text: Embryonic stem cell cultures are thought to self-organize into embryoid bodies,
able to undergo symmetry-breaking, germ layer specification and even morphogenesis.
Yet, it is unclear how to reconcile this remarkable self-organization capacity
with classical experiments demonstrating key roles for extrinsic biases by maternal
factors and/or extraembryonic tissues in embryogenesis. Here, we show that zebrafish
embryonic tissue explants, prepared prior to germ layer induction and lacking
extraembryonic tissues, can specify all germ layers and form a seemingly complete
mesendoderm anlage. Importantly, explant organization requires polarized inheritance
of maternal factors from dorsal-marginal regions of the blastoderm. Moreover,
induction of endoderm and head-mesoderm, which require peak Nodal-signaling levels,
is highly variable in explants, reminiscent of embryos with reduced Nodal signals
from the extraembryonic tissues. Together, these data suggest that zebrafish explants
do not undergo bona fide self-organization, but rather display features of genetically
encoded self-assembly, where intrinsic genetic programs control the emergence
of order.
article_number: e55190
article_processing_charge: No
article_type: original
author:
- first_name: Alexandra
full_name: Schauer, Alexandra
id: 30A536BA-F248-11E8-B48F-1D18A9856A87
last_name: Schauer
orcid: 0000-0001-7659-9142
- first_name: Diana C
full_name: Nunes Pinheiro, Diana C
id: 2E839F16-F248-11E8-B48F-1D18A9856A87
last_name: Nunes Pinheiro
orcid: 0000-0003-4333-7503
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Schauer A, Nunes Pinheiro DC, Hauschild R, Heisenberg C-PJ. Zebrafish embryonic
explants undergo genetically encoded self-assembly. eLife. 2020;9. doi:10.7554/elife.55190
apa: Schauer, A., Nunes Pinheiro, D. C., Hauschild, R., & Heisenberg, C.-P.
J. (2020). Zebrafish embryonic explants undergo genetically encoded self-assembly.
ELife. eLife Sciences Publications. https://doi.org/10.7554/elife.55190
chicago: Schauer, Alexandra, Diana C Nunes Pinheiro, Robert Hauschild, and Carl-Philipp
J Heisenberg. “Zebrafish Embryonic Explants Undergo Genetically Encoded Self-Assembly.”
ELife. eLife Sciences Publications, 2020. https://doi.org/10.7554/elife.55190.
ieee: A. Schauer, D. C. Nunes Pinheiro, R. Hauschild, and C.-P. J. Heisenberg, “Zebrafish
embryonic explants undergo genetically encoded self-assembly,” eLife, vol.
9. eLife Sciences Publications, 2020.
ista: Schauer A, Nunes Pinheiro DC, Hauschild R, Heisenberg C-PJ. 2020. Zebrafish
embryonic explants undergo genetically encoded self-assembly. eLife. 9, e55190.
mla: Schauer, Alexandra, et al. “Zebrafish Embryonic Explants Undergo Genetically
Encoded Self-Assembly.” ELife, vol. 9, e55190, eLife Sciences Publications,
2020, doi:10.7554/elife.55190.
short: A. Schauer, D.C. Nunes Pinheiro, R. Hauschild, C.-P.J. Heisenberg, ELife
9 (2020).
date_created: 2020-05-25T15:01:40Z
date_published: 2020-04-06T00:00:00Z
date_updated: 2023-08-21T06:25:49Z
day: '06'
ddc:
- '570'
department:
- _id: CaHe
- _id: Bio
doi: 10.7554/elife.55190
ec_funded: 1
external_id:
isi:
- '000531544400001'
pmid:
- '32250246'
file:
- access_level: open_access
checksum: f6aad884cf706846ae9357fcd728f8b5
content_type: application/pdf
creator: dernst
date_created: 2020-05-25T15:15:43Z
date_updated: 2020-07-14T12:48:04Z
file_id: '7890'
file_name: 2020_eLife_Schauer.pdf
file_size: 7744848
relation: main_file
file_date_updated: 2020-07-14T12:48:04Z
has_accepted_license: '1'
intvolume: ' 9'
isi: 1
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742573'
name: Interaction and feedback between cell mechanics and fate specification in
vertebrate gastrulation
- _id: 26B1E39C-B435-11E9-9278-68D0E5697425
grant_number: '25239'
name: 'Mesendoderm specification in zebrafish: The role of extraembryonic tissues'
- _id: 26520D1E-B435-11E9-9278-68D0E5697425
grant_number: ALTF 850-2017
name: Coordination of mesendoderm cell fate specification and internalization during
zebrafish gastrulation
- _id: 266BC5CE-B435-11E9-9278-68D0E5697425
grant_number: LT000429
name: Coordination of mesendoderm fate specification and internalization during
zebrafish gastrulation
publication: eLife
publication_identifier:
issn:
- 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
related_material:
record:
- id: '12891'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Zebrafish embryonic explants undergo genetically encoded self-assembly
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 9
year: '2020'
...
---
_id: '7877'
abstract:
- lang: eng
text: The NIPBL/MAU2 heterodimer loads cohesin onto chromatin. Mutations inNIPBLaccount
for most cases ofthe rare developmental disorder Cornelia de Lange syndrome (CdLS).
Here we report aMAU2 variant causing CdLS, a deletion of seven amino acids that
impairs the interaction between MAU2 and the NIPBL N terminus.Investigating this
interaction, we discovered that MAU2 and the NIPBL N terminus are largely dispensable
fornormal cohesin and NIPBL function in cells with a NIPBL early truncating mutation.
Despite a predicted fataloutcome of an out-of-frame single nucleotide duplication
inNIPBL, engineered in two different cell lines,alternative translation initiation
yields a form of NIPBL missing N-terminal residues. This form cannot interactwith
MAU2, but binds DNA and mediates cohesin loading. Altogether, our work reveals
that cohesin loading can occur independently of functional NIPBL/MAU2 complexes
and highlights a novel mechanism protectiveagainst out-of-frame mutations that
is potentially relevant for other genetic conditions.
article_number: '107647'
article_processing_charge: No
article_type: original
author:
- first_name: Ilaria
full_name: Parenti, Ilaria
id: D93538B0-5B71-11E9-AC62-02EBE5697425
last_name: Parenti
- first_name: Farah
full_name: Diab, Farah
last_name: Diab
- first_name: Sara Ruiz
full_name: Gil, Sara Ruiz
last_name: Gil
- first_name: Eskeatnaf
full_name: Mulugeta, Eskeatnaf
last_name: Mulugeta
- first_name: Valentina
full_name: Casa, Valentina
last_name: Casa
- first_name: Riccardo
full_name: Berutti, Riccardo
last_name: Berutti
- first_name: Rutger W.W.
full_name: Brouwer, Rutger W.W.
last_name: Brouwer
- first_name: Valerie
full_name: Dupé, Valerie
last_name: Dupé
- first_name: Juliane
full_name: Eckhold, Juliane
last_name: Eckhold
- first_name: Elisabeth
full_name: Graf, Elisabeth
last_name: Graf
- first_name: Beatriz
full_name: Puisac, Beatriz
last_name: Puisac
- first_name: Feliciano
full_name: Ramos, Feliciano
last_name: Ramos
- first_name: Thomas
full_name: Schwarzmayr, Thomas
last_name: Schwarzmayr
- first_name: Macarena Moronta
full_name: Gines, Macarena Moronta
last_name: Gines
- first_name: Thomas
full_name: Van Staveren, Thomas
last_name: Van Staveren
- first_name: Wilfred F.J.
full_name: Van Ijcken, Wilfred F.J.
last_name: Van Ijcken
- first_name: Tim M.
full_name: Strom, Tim M.
last_name: Strom
- first_name: Juan
full_name: Pié, Juan
last_name: Pié
- first_name: Erwan
full_name: Watrin, Erwan
last_name: Watrin
- first_name: Frank J.
full_name: Kaiser, Frank J.
last_name: Kaiser
- first_name: Kerstin S.
full_name: Wendt, Kerstin S.
last_name: Wendt
citation:
ama: Parenti I, Diab F, Gil SR, et al. MAU2 and NIPBL variants impair the heterodimerization
of the cohesin loader subunits and cause Cornelia de Lange syndrome. Cell Reports.
2020;31(7). doi:10.1016/j.celrep.2020.107647
apa: Parenti, I., Diab, F., Gil, S. R., Mulugeta, E., Casa, V., Berutti, R., … Wendt,
K. S. (2020). MAU2 and NIPBL variants impair the heterodimerization of the cohesin
loader subunits and cause Cornelia de Lange syndrome. Cell Reports. Elsevier.
https://doi.org/10.1016/j.celrep.2020.107647
chicago: Parenti, Ilaria, Farah Diab, Sara Ruiz Gil, Eskeatnaf Mulugeta, Valentina
Casa, Riccardo Berutti, Rutger W.W. Brouwer, et al. “MAU2 and NIPBL Variants Impair
the Heterodimerization of the Cohesin Loader Subunits and Cause Cornelia de Lange
Syndrome.” Cell Reports. Elsevier, 2020. https://doi.org/10.1016/j.celrep.2020.107647.
ieee: I. Parenti et al., “MAU2 and NIPBL variants impair the heterodimerization
of the cohesin loader subunits and cause Cornelia de Lange syndrome,” Cell
Reports, vol. 31, no. 7. Elsevier, 2020.
ista: Parenti I, Diab F, Gil SR, Mulugeta E, Casa V, Berutti R, Brouwer RWW, Dupé
V, Eckhold J, Graf E, Puisac B, Ramos F, Schwarzmayr T, Gines MM, Van Staveren
T, Van Ijcken WFJ, Strom TM, Pié J, Watrin E, Kaiser FJ, Wendt KS. 2020. MAU2
and NIPBL variants impair the heterodimerization of the cohesin loader subunits
and cause Cornelia de Lange syndrome. Cell Reports. 31(7), 107647.
mla: Parenti, Ilaria, et al. “MAU2 and NIPBL Variants Impair the Heterodimerization
of the Cohesin Loader Subunits and Cause Cornelia de Lange Syndrome.” Cell
Reports, vol. 31, no. 7, 107647, Elsevier, 2020, doi:10.1016/j.celrep.2020.107647.
short: I. Parenti, F. Diab, S.R. Gil, E. Mulugeta, V. Casa, R. Berutti, R.W.W. Brouwer,
V. Dupé, J. Eckhold, E. Graf, B. Puisac, F. Ramos, T. Schwarzmayr, M.M. Gines,
T. Van Staveren, W.F.J. Van Ijcken, T.M. Strom, J. Pié, E. Watrin, F.J. Kaiser,
K.S. Wendt, Cell Reports 31 (2020).
date_created: 2020-05-24T22:00:57Z
date_published: 2020-05-19T00:00:00Z
date_updated: 2023-08-21T06:27:47Z
day: '19'
ddc:
- '570'
department:
- _id: GaNo
doi: 10.1016/j.celrep.2020.107647
external_id:
isi:
- '000535655200005'
file:
- access_level: open_access
checksum: 64d8f7467731ee5c166b10b939b8310b
content_type: application/pdf
creator: dernst
date_created: 2020-05-26T11:05:01Z
date_updated: 2020-07-14T12:48:04Z
file_id: '7892'
file_name: 2020_CellReports_Parenti.pdf
file_size: 4695682
relation: main_file
file_date_updated: 2020-07-14T12:48:04Z
has_accepted_license: '1'
intvolume: ' 31'
isi: 1
issue: '7'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
publication: Cell Reports
publication_identifier:
eissn:
- '22111247'
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: MAU2 and NIPBL variants impair the heterodimerization of the cohesin loader
subunits and cause Cornelia de Lange syndrome
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 31
year: '2020'
...
---
_id: '7878'
abstract:
- lang: eng
text: Type 1 metabotropic glutamate receptors (mGluR1s) are key elements in neuronal
signaling. While their function is well documented in slices, requirements for
their activation in vivo are poorly understood. We examine this question in adult
mice in vivo using 2-photon imaging of cerebellar molecular layer interneurons
(MLIs) expressing GCaMP. In anesthetized mice, parallel fiber activation evokes
beam-like Cai rises in postsynaptic MLIs which depend on co-activation of mGluR1s
and ionotropic glutamate receptors (iGluRs). In awake mice, blocking mGluR1 decreases
Cai rises associated with locomotion. In vitro studies and freeze-fracture electron
microscopy show that the iGluR-mGluR1 interaction is synergistic and favored by
close association of the two classes of receptors. Altogether our results suggest
that mGluR1s, acting in synergy with iGluRs, potently contribute to processing
cerebellar neuronal signaling under physiological conditions.
article_number: e56839
article_processing_charge: No
article_type: original
author:
- first_name: Jin
full_name: Bao, Jin
last_name: Bao
- first_name: Michael
full_name: Graupner, Michael
last_name: Graupner
- first_name: Guadalupe
full_name: Astorga, Guadalupe
last_name: Astorga
- first_name: Thibault
full_name: Collin, Thibault
last_name: Collin
- first_name: Abdelali
full_name: Jalil, Abdelali
last_name: Jalil
- first_name: Dwi Wahyu
full_name: Indriati, Dwi Wahyu
last_name: Indriati
- first_name: Jonathan
full_name: Bradley, Jonathan
last_name: Bradley
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Isabel
full_name: Llano, Isabel
last_name: Llano
citation:
ama: Bao J, Graupner M, Astorga G, et al. Synergism of type 1 metabotropic and ionotropic
glutamate receptors in cerebellar molecular layer interneurons in vivo. eLife.
2020;9. doi:10.7554/eLife.56839
apa: Bao, J., Graupner, M., Astorga, G., Collin, T., Jalil, A., Indriati, D. W.,
… Llano, I. (2020). Synergism of type 1 metabotropic and ionotropic glutamate
receptors in cerebellar molecular layer interneurons in vivo. ELife. eLife
Sciences Publications. https://doi.org/10.7554/eLife.56839
chicago: Bao, Jin, Michael Graupner, Guadalupe Astorga, Thibault Collin, Abdelali
Jalil, Dwi Wahyu Indriati, Jonathan Bradley, Ryuichi Shigemoto, and Isabel Llano.
“Synergism of Type 1 Metabotropic and Ionotropic Glutamate Receptors in Cerebellar
Molecular Layer Interneurons in Vivo.” ELife. eLife Sciences Publications,
2020. https://doi.org/10.7554/eLife.56839.
ieee: J. Bao et al., “Synergism of type 1 metabotropic and ionotropic glutamate
receptors in cerebellar molecular layer interneurons in vivo,” eLife, vol.
9. eLife Sciences Publications, 2020.
ista: Bao J, Graupner M, Astorga G, Collin T, Jalil A, Indriati DW, Bradley J, Shigemoto
R, Llano I. 2020. Synergism of type 1 metabotropic and ionotropic glutamate receptors
in cerebellar molecular layer interneurons in vivo. eLife. 9, e56839.
mla: Bao, Jin, et al. “Synergism of Type 1 Metabotropic and Ionotropic Glutamate
Receptors in Cerebellar Molecular Layer Interneurons in Vivo.” ELife, vol.
9, e56839, eLife Sciences Publications, 2020, doi:10.7554/eLife.56839.
short: J. Bao, M. Graupner, G. Astorga, T. Collin, A. Jalil, D.W. Indriati, J. Bradley,
R. Shigemoto, I. Llano, ELife 9 (2020).
date_created: 2020-05-24T22:00:58Z
date_published: 2020-05-13T00:00:00Z
date_updated: 2023-08-21T06:26:50Z
day: '13'
ddc:
- '570'
department:
- _id: RySh
doi: 10.7554/eLife.56839
external_id:
isi:
- '000535191600001'
pmid:
- '32401196'
file:
- access_level: open_access
checksum: 8ea99bb6660cc407dbdb00c173b01683
content_type: application/pdf
creator: dernst
date_created: 2020-05-26T09:34:54Z
date_updated: 2020-07-14T12:48:04Z
file_id: '7891'
file_name: 2020_eLife_Bao.pdf
file_size: 4832050
relation: main_file
file_date_updated: 2020-07-14T12:48:04Z
has_accepted_license: '1'
intvolume: ' 9'
isi: 1
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
pmid: 1
publication: eLife
publication_identifier:
eissn:
- 2050084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: Synergism of type 1 metabotropic and ionotropic glutamate receptors in cerebellar
molecular layer interneurons in vivo
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 9
year: '2020'
...
---
_id: '7880'
abstract:
- lang: eng
text: 'Following its evoked release, dopamine (DA) signaling is rapidly terminated
by presynaptic reuptake, mediated by the cocaine-sensitive DA transporter (DAT).
DAT surface availability is dynamically regulated by endocytic trafficking, and
direct protein kinase C (PKC) activation acutely diminishes DAT surface expression
by accelerating DAT internalization. Previous cell line studies demonstrated that
PKC-stimulated DAT endocytosis requires both Ack1 inactivation, which releases
a DAT-specific endocytic brake, and the neuronal GTPase, Rit2, which binds DAT.
However, it is unknown whether Rit2 is required for PKC-stimulated DAT endocytosis
in DAergic terminals or whether there are region- and/or sex-dependent differences
in PKC-stimulated DAT trafficking. Moreover, the mechanisms by which Rit2 controls
PKC-stimulated DAT endocytosis are unknown. Here, we directly examined these important
questions. Ex vivo studies revealed that PKC activation acutely decreased DAT
surface expression selectively in ventral, but not dorsal, striatum. AAV-mediated,
conditional Rit2 knockdown in DAergic neurons impacted baseline DAT surface:intracellular
distribution in DAergic terminals from female ventral, but not dorsal, striatum.
Further, Rit2 was required for PKC-stimulated DAT internalization in both male
and female ventral striatum. FRET and surface pulldown studies in cell lines revealed
that PKC activation drives DAT-Rit2 surface dissociation and that the DAT N terminus
is required for both PKC-mediated DAT-Rit2 dissociation and DAT internalization.
Finally, we found that Rit2 and Ack1 independently converge on DAT to facilitate
PKC-stimulated DAT endocytosis. Together, our data provide greater insight into
mechanisms that mediate PKC-regulated DAT internalization and reveal unexpected
region-specific differences in PKC-stimulated DAT trafficking in bona fide DAergic
terminals. '
article_processing_charge: No
article_type: original
author:
- first_name: Rita R.
full_name: Fagan, Rita R.
last_name: Fagan
- first_name: Patrick J.
full_name: Kearney, Patrick J.
last_name: Kearney
- first_name: Carolyn G.
full_name: Sweeney, Carolyn G.
last_name: Sweeney
- first_name: Dino
full_name: Luethi, Dino
last_name: Luethi
- first_name: Florianne E
full_name: Schoot Uiterkamp, Florianne E
id: 3526230C-F248-11E8-B48F-1D18A9856A87
last_name: Schoot Uiterkamp
- first_name: Klaus
full_name: Schicker, Klaus
last_name: Schicker
- first_name: Brian S.
full_name: Alejandro, Brian S.
last_name: Alejandro
- first_name: Lauren C.
full_name: O'Connor, Lauren C.
last_name: O'Connor
- first_name: Harald H.
full_name: Sitte, Harald H.
last_name: Sitte
- first_name: Haley E.
full_name: Melikian, Haley E.
last_name: Melikian
citation:
ama: 'Fagan RR, Kearney PJ, Sweeney CG, et al. Dopamine transporter trafficking
and Rit2 GTPase: Mechanism of action and in vivo impact. Journal of Biological
Chemistry. 2020;295(16):5229-5244. doi:10.1074/jbc.RA120.012628'
apa: 'Fagan, R. R., Kearney, P. J., Sweeney, C. G., Luethi, D., Schoot Uiterkamp,
F. E., Schicker, K., … Melikian, H. E. (2020). Dopamine transporter trafficking
and Rit2 GTPase: Mechanism of action and in vivo impact. Journal of Biological
Chemistry. ASBMB Publications. https://doi.org/10.1074/jbc.RA120.012628'
chicago: 'Fagan, Rita R., Patrick J. Kearney, Carolyn G. Sweeney, Dino Luethi, Florianne
E Schoot Uiterkamp, Klaus Schicker, Brian S. Alejandro, Lauren C. O’Connor, Harald
H. Sitte, and Haley E. Melikian. “Dopamine Transporter Trafficking and Rit2 GTPase:
Mechanism of Action and in Vivo Impact.” Journal of Biological Chemistry.
ASBMB Publications, 2020. https://doi.org/10.1074/jbc.RA120.012628.'
ieee: 'R. R. Fagan et al., “Dopamine transporter trafficking and Rit2 GTPase:
Mechanism of action and in vivo impact,” Journal of Biological Chemistry,
vol. 295, no. 16. ASBMB Publications, pp. 5229–5244, 2020.'
ista: 'Fagan RR, Kearney PJ, Sweeney CG, Luethi D, Schoot Uiterkamp FE, Schicker
K, Alejandro BS, O’Connor LC, Sitte HH, Melikian HE. 2020. Dopamine transporter
trafficking and Rit2 GTPase: Mechanism of action and in vivo impact. Journal of
Biological Chemistry. 295(16), 5229–5244.'
mla: 'Fagan, Rita R., et al. “Dopamine Transporter Trafficking and Rit2 GTPase:
Mechanism of Action and in Vivo Impact.” Journal of Biological Chemistry,
vol. 295, no. 16, ASBMB Publications, 2020, pp. 5229–44, doi:10.1074/jbc.RA120.012628.'
short: R.R. Fagan, P.J. Kearney, C.G. Sweeney, D. Luethi, F.E. Schoot Uiterkamp,
K. Schicker, B.S. Alejandro, L.C. O’Connor, H.H. Sitte, H.E. Melikian, Journal
of Biological Chemistry 295 (2020) 5229–5244.
date_created: 2020-05-24T22:00:59Z
date_published: 2020-04-17T00:00:00Z
date_updated: 2023-08-21T06:26:22Z
day: '17'
department:
- _id: SaSi
doi: 10.1074/jbc.RA120.012628
external_id:
isi:
- '000530288000006'
pmid:
- '32132171'
intvolume: ' 295'
isi: 1
issue: '16'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://escholarship.umassmed.edu/oapubs/4187
month: '04'
oa: 1
oa_version: Submitted Version
page: 5229-5244
pmid: 1
publication: Journal of Biological Chemistry
publication_identifier:
eissn:
- 1083351X
issn:
- '00219258'
publication_status: published
publisher: ASBMB Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Dopamine transporter trafficking and Rit2 GTPase: Mechanism of action and
in vivo impact'
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 295
year: '2020'
...
---
_id: '7864'
abstract:
- lang: eng
text: "Purpose of review: Cancer is one of the leading causes of death and the incidence
rates are constantly rising. The heterogeneity of tumors poses a big challenge
for the treatment of the disease and natural antibodies additionally affect disease
progression. The introduction of engineered mAbs for anticancer immunotherapies
has substantially improved progression-free and overall survival of cancer patients,
but little efforts have been made to exploit other antibody isotypes than IgG.\r\nRecent
findings: In order to improve these therapies, ‘next-generation antibodies’ were
engineered to enhance a specific feature of classical antibodies and form a group
of highly effective and precise therapy compounds. Advanced antibody approaches
include among others antibody-drug conjugates, glyco-engineered and Fc-engineered
antibodies, antibody fragments, radioimmunotherapy compounds, bispecific antibodies
and alternative (non-IgG) immunoglobulin classes, especially IgE.\r\nSummary:
The current review describes solutions for the needs of next-generation antibody
therapies through different approaches. Careful selection of the best-suited engineering
methodology is a key factor in developing personalized, more specific and more
efficient mAbs against cancer to improve the outcomes of cancer patients. We highlight
here the large evidence of IgE exploiting a highly cytotoxic effector arm as potential
next-generation anticancer immunotherapy."
article_processing_charge: No
article_type: original
author:
- first_name: Judit
full_name: Singer, Judit
id: 36432834-F248-11E8-B48F-1D18A9856A87
last_name: Singer
orcid: 0000-0002-8777-3502
- first_name: Josef
full_name: Singer, Josef
last_name: Singer
- first_name: Erika
full_name: Jensen-Jarolim, Erika
last_name: Jensen-Jarolim
citation:
ama: 'Singer J, Singer J, Jensen-Jarolim E. Precision medicine in clinical oncology:
the journey from IgG antibody to IgE. Current opinion in allergy and clinical
immunology. 2020;20(3):282-289. doi:10.1097/ACI.0000000000000637'
apa: 'Singer, J., Singer, J., & Jensen-Jarolim, E. (2020). Precision medicine
in clinical oncology: the journey from IgG antibody to IgE. Current Opinion
in Allergy and Clinical Immunology. Wolters Kluwer. https://doi.org/10.1097/ACI.0000000000000637'
chicago: 'Singer, Judit, Josef Singer, and Erika Jensen-Jarolim. “Precision Medicine
in Clinical Oncology: The Journey from IgG Antibody to IgE.” Current Opinion
in Allergy and Clinical Immunology. Wolters Kluwer, 2020. https://doi.org/10.1097/ACI.0000000000000637.'
ieee: 'J. Singer, J. Singer, and E. Jensen-Jarolim, “Precision medicine in clinical
oncology: the journey from IgG antibody to IgE,” Current opinion in allergy
and clinical immunology, vol. 20, no. 3. Wolters Kluwer, pp. 282–289, 2020.'
ista: 'Singer J, Singer J, Jensen-Jarolim E. 2020. Precision medicine in clinical
oncology: the journey from IgG antibody to IgE. Current opinion in allergy and
clinical immunology. 20(3), 282–289.'
mla: 'Singer, Judit, et al. “Precision Medicine in Clinical Oncology: The Journey
from IgG Antibody to IgE.” Current Opinion in Allergy and Clinical Immunology,
vol. 20, no. 3, Wolters Kluwer, 2020, pp. 282–89, doi:10.1097/ACI.0000000000000637.'
short: J. Singer, J. Singer, E. Jensen-Jarolim, Current Opinion in Allergy and Clinical
Immunology 20 (2020) 282–289.
date_created: 2020-05-17T22:00:44Z
date_published: 2020-06-01T00:00:00Z
date_updated: 2023-08-21T06:28:52Z
day: '01'
department:
- _id: Bio
doi: 10.1097/ACI.0000000000000637
external_id:
isi:
- '000561358300010'
intvolume: ' 20'
isi: 1
issue: '3'
language:
- iso: eng
month: '06'
oa_version: None
page: 282-289
publication: Current opinion in allergy and clinical immunology
publication_identifier:
eissn:
- '14736322'
publication_status: published
publisher: Wolters Kluwer
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Precision medicine in clinical oncology: the journey from IgG antibody to
IgE'
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 20
year: '2020'
...