---
_id: '15057'
abstract:
- lang: eng
text: Vaccinia virus–related kinase (VRK) is an evolutionarily conserved nuclear
protein kinase. VRK-1, the single Caenorhabditis elegans VRK ortholog, functions
in cell division and germline proliferation. However, the role of VRK-1 in postmitotic
cells and adult life span remains unknown. Here, we show that VRK-1 increases
organismal longevity by activating the cellular energy sensor, AMP-activated protein
kinase (AMPK), via direct phosphorylation. We found that overexpression of vrk-1
in the soma of adult C. elegans increased life span and, conversely, inhibition
of vrk-1 decreased life span. In addition, vrk-1 was required for longevity conferred
by mutations that inhibit C. elegans mitochondrial respiration, which requires
AMPK. VRK-1 directly phosphorylated and up-regulated AMPK in both C. elegans and
cultured human cells. Thus, our data show that the somatic nuclear kinase, VRK-1,
promotes longevity through AMPK activation, and this function appears to be conserved
between C. elegans and humans.
acknowledgement: 'This research was supported by grants NRF-2019R1A3B2067745 and NRF-2017R1A5A1015366
funded by the Korean Government (MSIT) through the National Research Foundation
(NRF) of Korea to S.-J.V.L. and by grant Basic Science Research Program (No. 2019R1A2C2009440)
funded by the Korean Government (MSIT) through the NRF of Korea to K.-T.K. '
article_number: aaw7824
article_processing_charge: No
article_type: original
author:
- first_name: Sangsoon
full_name: Park, Sangsoon
last_name: Park
- first_name: Murat
full_name: Artan, Murat
id: C407B586-6052-11E9-B3AE-7006E6697425
last_name: Artan
orcid: 0000-0001-8945-6992
- first_name: Seung Hyun
full_name: Han, Seung Hyun
last_name: Han
- first_name: Hae-Eun H.
full_name: Park, Hae-Eun H.
last_name: Park
- first_name: Yoonji
full_name: Jung, Yoonji
last_name: Jung
- first_name: Ara B.
full_name: Hwang, Ara B.
last_name: Hwang
- first_name: Won Sik
full_name: Shin, Won Sik
last_name: Shin
- first_name: Kyong-Tai
full_name: Kim, Kyong-Tai
last_name: Kim
- first_name: Seung-Jae V.
full_name: Lee, Seung-Jae V.
last_name: Lee
citation:
ama: Park S, Artan M, Han SH, et al. VRK-1 extends life span by activation of AMPK
via phosphorylation. Science Advances. 2020;6(27). doi:10.1126/sciadv.aaw7824
apa: Park, S., Artan, M., Han, S. H., Park, H.-E. H., Jung, Y., Hwang, A. B., …
Lee, S.-J. V. (2020). VRK-1 extends life span by activation of AMPK via phosphorylation.
Science Advances. American Association for the Advancement of Science.
https://doi.org/10.1126/sciadv.aaw7824
chicago: Park, Sangsoon, Murat Artan, Seung Hyun Han, Hae-Eun H. Park, Yoonji Jung,
Ara B. Hwang, Won Sik Shin, Kyong-Tai Kim, and Seung-Jae V. Lee. “VRK-1 Extends
Life Span by Activation of AMPK via Phosphorylation.” Science Advances.
American Association for the Advancement of Science, 2020. https://doi.org/10.1126/sciadv.aaw7824.
ieee: S. Park et al., “VRK-1 extends life span by activation of AMPK via
phosphorylation,” Science Advances, vol. 6, no. 27. American Association
for the Advancement of Science, 2020.
ista: Park S, Artan M, Han SH, Park H-EH, Jung Y, Hwang AB, Shin WS, Kim K-T, Lee
S-JV. 2020. VRK-1 extends life span by activation of AMPK via phosphorylation.
Science Advances. 6(27), aaw7824.
mla: Park, Sangsoon, et al. “VRK-1 Extends Life Span by Activation of AMPK via Phosphorylation.”
Science Advances, vol. 6, no. 27, aaw7824, American Association for the
Advancement of Science, 2020, doi:10.1126/sciadv.aaw7824.
short: S. Park, M. Artan, S.H. Han, H.-E.H. Park, Y. Jung, A.B. Hwang, W.S. Shin,
K.-T. Kim, S.-J.V. Lee, Science Advances 6 (2020).
date_created: 2024-03-04T09:41:57Z
date_published: 2020-07-01T00:00:00Z
date_updated: 2024-03-04T09:52:09Z
day: '01'
ddc:
- '570'
department:
- _id: MaDe
doi: 10.1126/sciadv.aaw7824
file:
- access_level: open_access
checksum: a37157cd0de709dce5fe03f4a31cd0b6
content_type: application/pdf
creator: dernst
date_created: 2024-03-04T09:46:41Z
date_updated: 2024-03-04T09:46:41Z
file_id: '15058'
file_name: 2020_ScienceAdvances_Park.pdf
file_size: 1864415
relation: main_file
success: 1
file_date_updated: 2024-03-04T09:46:41Z
has_accepted_license: '1'
intvolume: ' 6'
issue: '27'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc/4.0/
month: '07'
oa: 1
oa_version: Published Version
publication: Science Advances
publication_identifier:
eissn:
- 2375-2548
publication_status: published
publisher: American Association for the Advancement of Science
quality_controlled: '1'
status: public
title: VRK-1 extends life span by activation of AMPK via phosphorylation
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 6
year: '2020'
...
---
_id: '15061'
abstract:
- lang: eng
text: The actin cytoskeleton, a dynamic network of actin filaments and associated
F-actin–binding proteins, is fundamentally important in eukaryotes. α-Actinins
are major F-actin bundlers that are inhibited by Ca2+ in nonmuscle cells. Here
we report the mechanism of Ca2+-mediated regulation of Entamoeba histolytica α-actinin-2
(EhActn2) with features expected for the common ancestor of Entamoeba and higher
eukaryotic α-actinins. Crystal structures of Ca2+-free and Ca2+-bound EhActn2
reveal a calmodulin-like domain (CaMD) uniquely inserted within the rod domain.
Integrative studies reveal an exceptionally high affinity of the EhActn2 CaMD
for Ca2+, binding of which can only be regulated in the presence of physiological
concentrations of Mg2+. Ca2+ binding triggers an increase in protein multidomain
rigidity, reducing conformational flexibility of F-actin–binding domains via interdomain
cross-talk and consequently inhibiting F-actin bundling. In vivo studies uncover
that EhActn2 plays an important role in phagocytic cup formation and might constitute
a new drug target for amoebic dysentery.
acknowledged_ssus:
- _id: LifeSc
acknowledgement: "We thank the staff of the macromolecular crystallography (MX) and
SAXS beamlines at the European Synchrotron Radiation facility, Diamond, and Swiss
Light Source for excellent support, and the Life Sciences Facility of the Institute
of Science and Technology Austria for usage of the rheometer. We thank Life Sciences
editors for editing assistance. EM data were\r\nrecorded at the EM Facility of the
Vienna BioCenter Core Facilities (Austria). Confocal microscopy was carried out
at the Advanced Instrument Research Facility, Jawaharlal Nehru University. K.D.-C.’s
research was supported by the Initial Training Network MUZIC (ITN-MUZIC) (N°238423),
Austrian Science Fund (FWF) Projects I525, I1593, P22276, P19060, and W1221, Laura
Bassi Centre of Optimized Structural Studies (N°253275), a Wellcome Trust Collaborative
Award (201543/Z/16/Z), COST Action BM1405, Vienna Science and Technology Fund (WWTF)
Chemical Biology Project LS17-008, and Christian Doppler Laboratory for High-Content
Structural Biology and Biotechnology. K.Z., J.L.A., C.S., E.A.G., and A.S. were
supported by the University of Vienna, J.K. by a Wellcome Trust Collaborative Award
and by the Centre of Optimized Structural Studies, M.P. by FWF Project I1593, E.d.A.R.
ITN-MUZIC, and FWF Projects I525 and I1593, and T.C.M. and L.C. by FWF Project I
2408-B22. E.A.G. acknowledges the PhD program Structure and Interaction of Biological
Macromolecules. M.B. acknowledges the University Grant Commission, India, for a
senior research fellowship. A.B. acknowledges a JC Bose Fellowship from the Science
Engineering Research Council. "
article_processing_charge: No
article_type: original
author:
- first_name: Nikos
full_name: Pinotsis, Nikos
last_name: Pinotsis
- first_name: Karolina
full_name: Zielinska, Karolina
last_name: Zielinska
- first_name: Mrigya
full_name: Babuta, Mrigya
last_name: Babuta
- first_name: Joan L.
full_name: Arolas, Joan L.
last_name: Arolas
- first_name: Julius
full_name: Kostan, Julius
last_name: Kostan
- first_name: Muhammad Bashir
full_name: Khan, Muhammad Bashir
last_name: Khan
- first_name: Claudia
full_name: Schreiner, Claudia
last_name: Schreiner
- first_name: Anita P
full_name: Testa Salmazo, Anita P
id: 41F1F098-F248-11E8-B48F-1D18A9856A87
last_name: Testa Salmazo
- first_name: Luciano
full_name: Ciccarelli, Luciano
last_name: Ciccarelli
- first_name: Martin
full_name: Puchinger, Martin
last_name: Puchinger
- first_name: Eirini A.
full_name: Gkougkoulia, Eirini A.
last_name: Gkougkoulia
- first_name: Euripedes de Almeida
full_name: Ribeiro, Euripedes de Almeida
last_name: Ribeiro
- first_name: Thomas C.
full_name: Marlovits, Thomas C.
last_name: Marlovits
- first_name: Alok
full_name: Bhattacharya, Alok
last_name: Bhattacharya
- first_name: Kristina
full_name: Djinovic-Carugo, Kristina
last_name: Djinovic-Carugo
citation:
ama: Pinotsis N, Zielinska K, Babuta M, et al. Calcium modulates the domain flexibility
and function of an α-actinin similar to the ancestral α-actinin. Proceedings
of the National Academy of Sciences. 2020;117(36):22101-22112. doi:10.1073/pnas.1917269117
apa: Pinotsis, N., Zielinska, K., Babuta, M., Arolas, J. L., Kostan, J., Khan, M.
B., … Djinovic-Carugo, K. (2020). Calcium modulates the domain flexibility and
function of an α-actinin similar to the ancestral α-actinin. Proceedings of
the National Academy of Sciences. Proceedings of the National Academy of Sciences.
https://doi.org/10.1073/pnas.1917269117
chicago: Pinotsis, Nikos, Karolina Zielinska, Mrigya Babuta, Joan L. Arolas, Julius
Kostan, Muhammad Bashir Khan, Claudia Schreiner, et al. “Calcium Modulates the
Domain Flexibility and Function of an α-Actinin Similar to the Ancestral α-Actinin.”
Proceedings of the National Academy of Sciences. Proceedings of the National
Academy of Sciences, 2020. https://doi.org/10.1073/pnas.1917269117.
ieee: N. Pinotsis et al., “Calcium modulates the domain flexibility and function
of an α-actinin similar to the ancestral α-actinin,” Proceedings of the National
Academy of Sciences, vol. 117, no. 36. Proceedings of the National Academy
of Sciences, pp. 22101–22112, 2020.
ista: Pinotsis N, Zielinska K, Babuta M, Arolas JL, Kostan J, Khan MB, Schreiner
C, Testa Salmazo AP, Ciccarelli L, Puchinger M, Gkougkoulia EA, Ribeiro E de A,
Marlovits TC, Bhattacharya A, Djinovic-Carugo K. 2020. Calcium modulates the domain
flexibility and function of an α-actinin similar to the ancestral α-actinin. Proceedings
of the National Academy of Sciences. 117(36), 22101–22112.
mla: Pinotsis, Nikos, et al. “Calcium Modulates the Domain Flexibility and Function
of an α-Actinin Similar to the Ancestral α-Actinin.” Proceedings of the National
Academy of Sciences, vol. 117, no. 36, Proceedings of the National Academy
of Sciences, 2020, pp. 22101–12, doi:10.1073/pnas.1917269117.
short: N. Pinotsis, K. Zielinska, M. Babuta, J.L. Arolas, J. Kostan, M.B. Khan,
C. Schreiner, A.P. Testa Salmazo, L. Ciccarelli, M. Puchinger, E.A. Gkougkoulia,
E. de A. Ribeiro, T.C. Marlovits, A. Bhattacharya, K. Djinovic-Carugo, Proceedings
of the National Academy of Sciences 117 (2020) 22101–22112.
date_created: 2024-03-04T10:03:52Z
date_published: 2020-09-08T00:00:00Z
date_updated: 2024-03-04T10:14:44Z
day: '08'
department:
- _id: CaBe
doi: 10.1073/pnas.1917269117
external_id:
pmid:
- '32848067'
intvolume: ' 117'
issue: '36'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1073/pnas.191726911
month: '09'
oa: 1
oa_version: Published Version
page: 22101-22112
pmid: 1
publication: Proceedings of the National Academy of Sciences
publication_identifier:
eissn:
- 1091-6490
issn:
- 0027-8424
publication_status: published
publisher: Proceedings of the National Academy of Sciences
quality_controlled: '1'
status: public
title: Calcium modulates the domain flexibility and function of an α-actinin similar
to the ancestral α-actinin
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 117
year: '2020'
...
---
_id: '15064'
abstract:
- lang: eng
text: We call a continuous self-map that reveals itself through a discrete set of
point-value pairs a sampled dynamical system. Capturing the available information
with chain maps on Delaunay complexes, we use persistent homology to quantify
the evidence of recurrent behavior. We establish a sampling theorem to recover
the eigenspaces of the endomorphism on homology induced by the self-map. Using
a combinatorial gradient flow arising from the discrete Morse theory for Čech
and Delaunay complexes, we construct a chain map to transform the problem from
the natural but expensive Čech complexes to the computationally efficient Delaunay
triangulations. The fast chain map algorithm has applications beyond dynamical
systems.
acknowledgement: This research has been supported by the DFG Collaborative Research
Center SFB/TRR 109 “Discretization in Geometry and Dynamics”, by Polish MNiSzW Grant
No. 2621/7.PR/12/2013/2, by the Polish National Science Center under Maestro Grant
No. 2014/14/A/ST1/00453 and Grant No. DEC-2013/09/N/ST6/02995. Open Access funding
provided by Projekt DEAL.
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: U.
full_name: Bauer, U.
last_name: Bauer
- first_name: Herbert
full_name: Edelsbrunner, Herbert
id: 3FB178DA-F248-11E8-B48F-1D18A9856A87
last_name: Edelsbrunner
orcid: 0000-0002-9823-6833
- first_name: Grzegorz
full_name: Jablonski, Grzegorz
id: 4483EF78-F248-11E8-B48F-1D18A9856A87
last_name: Jablonski
orcid: 0000-0002-3536-9866
- first_name: M.
full_name: Mrozek, M.
last_name: Mrozek
citation:
ama: Bauer U, Edelsbrunner H, Jablonski G, Mrozek M. Čech-Delaunay gradient flow
and homology inference for self-maps. Journal of Applied and Computational
Topology. 2020;4(4):455-480. doi:10.1007/s41468-020-00058-8
apa: Bauer, U., Edelsbrunner, H., Jablonski, G., & Mrozek, M. (2020). Čech-Delaunay
gradient flow and homology inference for self-maps. Journal of Applied and
Computational Topology. Springer Nature. https://doi.org/10.1007/s41468-020-00058-8
chicago: Bauer, U., Herbert Edelsbrunner, Grzegorz Jablonski, and M. Mrozek. “Čech-Delaunay
Gradient Flow and Homology Inference for Self-Maps.” Journal of Applied and
Computational Topology. Springer Nature, 2020. https://doi.org/10.1007/s41468-020-00058-8.
ieee: U. Bauer, H. Edelsbrunner, G. Jablonski, and M. Mrozek, “Čech-Delaunay gradient
flow and homology inference for self-maps,” Journal of Applied and Computational
Topology, vol. 4, no. 4. Springer Nature, pp. 455–480, 2020.
ista: Bauer U, Edelsbrunner H, Jablonski G, Mrozek M. 2020. Čech-Delaunay gradient
flow and homology inference for self-maps. Journal of Applied and Computational
Topology. 4(4), 455–480.
mla: Bauer, U., et al. “Čech-Delaunay Gradient Flow and Homology Inference for Self-Maps.”
Journal of Applied and Computational Topology, vol. 4, no. 4, Springer
Nature, 2020, pp. 455–80, doi:10.1007/s41468-020-00058-8.
short: U. Bauer, H. Edelsbrunner, G. Jablonski, M. Mrozek, Journal of Applied and
Computational Topology 4 (2020) 455–480.
date_created: 2024-03-04T10:47:49Z
date_published: 2020-12-01T00:00:00Z
date_updated: 2024-03-04T10:54:04Z
day: '01'
ddc:
- '500'
department:
- _id: HeEd
doi: 10.1007/s41468-020-00058-8
file:
- access_level: open_access
checksum: eed1168b6e66cd55272c19bb7fca8a1c
content_type: application/pdf
creator: dernst
date_created: 2024-03-04T10:52:42Z
date_updated: 2024-03-04T10:52:42Z
file_id: '15065'
file_name: 2020_JourApplCompTopology_Bauer.pdf
file_size: 851190
relation: main_file
success: 1
file_date_updated: 2024-03-04T10:52:42Z
has_accepted_license: '1'
intvolume: ' 4'
issue: '4'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '12'
oa: 1
oa_version: Published Version
page: 455-480
publication: Journal of Applied and Computational Topology
publication_identifier:
eissn:
- 2367-1734
issn:
- 2367-1726
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Čech-Delaunay gradient flow and homology inference for self-maps
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 4
year: '2020'
...
---
_id: '15063'
abstract:
- lang: eng
text: We consider the least singular value of a large random matrix with real or
complex i.i.d. Gaussian entries shifted by a constant z∈C. We prove an optimal
lower tail estimate on this singular value in the critical regime where z is around
the spectral edge, thus improving the classical bound of Sankar, Spielman and
Teng (SIAM J. Matrix Anal. Appl. 28:2 (2006), 446–476) for the particular shift-perturbation
in the edge regime. Lacking Brézin–Hikami formulas in the real case, we rely on
the superbosonization formula (Comm. Math. Phys. 283:2 (2008), 343–395).
acknowledgement: Partially supported by ERC Advanced Grant No. 338804. This project
has received funding from the European Union’s Horizon 2020 research and innovation
programme under the Marie Sklodowska-Curie Grant Agreement No. 66538
article_processing_charge: No
article_type: original
author:
- first_name: Giorgio
full_name: Cipolloni, Giorgio
id: 42198EFA-F248-11E8-B48F-1D18A9856A87
last_name: Cipolloni
orcid: 0000-0002-4901-7992
- first_name: László
full_name: Erdös, László
id: 4DBD5372-F248-11E8-B48F-1D18A9856A87
last_name: Erdös
orcid: 0000-0001-5366-9603
- first_name: Dominik J
full_name: Schröder, Dominik J
id: 408ED176-F248-11E8-B48F-1D18A9856A87
last_name: Schröder
orcid: 0000-0002-2904-1856
citation:
ama: Cipolloni G, Erdös L, Schröder DJ. Optimal lower bound on the least singular
value of the shifted Ginibre ensemble. Probability and Mathematical Physics.
2020;1(1):101-146. doi:10.2140/pmp.2020.1.101
apa: Cipolloni, G., Erdös, L., & Schröder, D. J. (2020). Optimal lower bound
on the least singular value of the shifted Ginibre ensemble. Probability and
Mathematical Physics. Mathematical Sciences Publishers. https://doi.org/10.2140/pmp.2020.1.101
chicago: Cipolloni, Giorgio, László Erdös, and Dominik J Schröder. “Optimal Lower
Bound on the Least Singular Value of the Shifted Ginibre Ensemble.” Probability
and Mathematical Physics. Mathematical Sciences Publishers, 2020. https://doi.org/10.2140/pmp.2020.1.101.
ieee: G. Cipolloni, L. Erdös, and D. J. Schröder, “Optimal lower bound on the least
singular value of the shifted Ginibre ensemble,” Probability and Mathematical
Physics, vol. 1, no. 1. Mathematical Sciences Publishers, pp. 101–146, 2020.
ista: Cipolloni G, Erdös L, Schröder DJ. 2020. Optimal lower bound on the least
singular value of the shifted Ginibre ensemble. Probability and Mathematical Physics.
1(1), 101–146.
mla: Cipolloni, Giorgio, et al. “Optimal Lower Bound on the Least Singular Value
of the Shifted Ginibre Ensemble.” Probability and Mathematical Physics,
vol. 1, no. 1, Mathematical Sciences Publishers, 2020, pp. 101–46, doi:10.2140/pmp.2020.1.101.
short: G. Cipolloni, L. Erdös, D.J. Schröder, Probability and Mathematical Physics
1 (2020) 101–146.
date_created: 2024-03-04T10:27:57Z
date_published: 2020-11-16T00:00:00Z
date_updated: 2024-03-04T10:33:15Z
day: '16'
department:
- _id: LaEr
doi: 10.2140/pmp.2020.1.101
ec_funded: 1
external_id:
arxiv:
- '1908.01653'
intvolume: ' 1'
issue: '1'
keyword:
- General Medicine
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.48550/arXiv.1908.01653
month: '11'
oa: 1
oa_version: Preprint
page: 101-146
project:
- _id: 258DCDE6-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '338804'
name: Random matrices, universality and disordered quantum systems
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
publication: Probability and Mathematical Physics
publication_identifier:
issn:
- 2690-1005
- 2690-0998
publication_status: published
publisher: Mathematical Sciences Publishers
quality_controlled: '1'
scopus_import: '1'
status: public
title: Optimal lower bound on the least singular value of the shifted Ginibre ensemble
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 1
year: '2020'
...
---
_id: '15059'
abstract:
- lang: eng
text: "In this paper we present a room temperature radiometer that can eliminate
the need of using cryostats in satellite payload reducing its weight and improving
reliability. The proposed radiometer is based on an electro-optic upconverter
that boosts up microwave photons energy by upconverting them into an optical domain
what makes them immune to thermal noise even if operating at room temperature.
The converter uses a high-quality factor whispering gallery\r\nmode (WGM) resonator
providing naturally narrow bandwidth and therefore might be useful for applications
like microwave hyperspectral sensing. The upconversion process is explained by\r\nproviding
essential information about photon conversion efficiency and sensitivity. To prove
the concept, we describe an experiment which shows state-of-the-art photon conversion
efficiency n=10-5 per mW of pump power at the frequency of 80 GHz."
acknowledgement: This work has been financially supported by Comunidad de Madrid S2018/NMT-4333
ARTINLARA-CM projects, and “FUNDACIÓN SENER” REFTA projects.
article_processing_charge: No
author:
- first_name: Michal
full_name: Wasiak, Michal
last_name: Wasiak
- first_name: Gabriel Santamaria
full_name: Botello, Gabriel Santamaria
last_name: Botello
- first_name: Kerlos Atia
full_name: Abdalmalak, Kerlos Atia
last_name: Abdalmalak
- first_name: Florian
full_name: Sedlmeir, Florian
last_name: Sedlmeir
- first_name: Alfredo R
full_name: Rueda Sanchez, Alfredo R
id: 3B82B0F8-F248-11E8-B48F-1D18A9856A87
last_name: Rueda Sanchez
orcid: 0000-0001-6249-5860
- first_name: Daniel
full_name: Segovia-Vargas, Daniel
last_name: Segovia-Vargas
- first_name: Harald G. L.
full_name: Schwefel, Harald G. L.
last_name: Schwefel
- first_name: Luis Enrique Garcia
full_name: Munoz, Luis Enrique Garcia
last_name: Munoz
citation:
ama: 'Wasiak M, Botello GS, Abdalmalak KA, et al. Compact millimeter and submillimeter-wave
photonic radiometer for cubesats. In: 14th European Conference on Antennas
and Propagation. IEEE; 2020. doi:10.23919/eucap48036.2020.9135962'
apa: 'Wasiak, M., Botello, G. S., Abdalmalak, K. A., Sedlmeir, F., Rueda Sanchez,
A. R., Segovia-Vargas, D., … Munoz, L. E. G. (2020). Compact millimeter and submillimeter-wave
photonic radiometer for cubesats. In 14th European Conference on Antennas and
Propagation. Copenhagen, Denmark: IEEE. https://doi.org/10.23919/eucap48036.2020.9135962'
chicago: Wasiak, Michal, Gabriel Santamaria Botello, Kerlos Atia Abdalmalak, Florian
Sedlmeir, Alfredo R Rueda Sanchez, Daniel Segovia-Vargas, Harald G. L. Schwefel,
and Luis Enrique Garcia Munoz. “Compact Millimeter and Submillimeter-Wave Photonic
Radiometer for Cubesats.” In 14th European Conference on Antennas and Propagation.
IEEE, 2020. https://doi.org/10.23919/eucap48036.2020.9135962.
ieee: M. Wasiak et al., “Compact millimeter and submillimeter-wave photonic
radiometer for cubesats,” in 14th European Conference on Antennas and Propagation,
Copenhagen, Denmark, 2020.
ista: 'Wasiak M, Botello GS, Abdalmalak KA, Sedlmeir F, Rueda Sanchez AR, Segovia-Vargas
D, Schwefel HGL, Munoz LEG. 2020. Compact millimeter and submillimeter-wave photonic
radiometer for cubesats. 14th European Conference on Antennas and Propagation.
EuCAP: European Conference on Antennas and Propagation.'
mla: Wasiak, Michal, et al. “Compact Millimeter and Submillimeter-Wave Photonic
Radiometer for Cubesats.” 14th European Conference on Antennas and Propagation,
IEEE, 2020, doi:10.23919/eucap48036.2020.9135962.
short: M. Wasiak, G.S. Botello, K.A. Abdalmalak, F. Sedlmeir, A.R. Rueda Sanchez,
D. Segovia-Vargas, H.G.L. Schwefel, L.E.G. Munoz, in:, 14th European Conference
on Antennas and Propagation, IEEE, 2020.
conference:
end_date: 2020-03-20
location: Copenhagen, Denmark
name: 'EuCAP: European Conference on Antennas and Propagation'
start_date: 2020-03-15
date_created: 2024-03-04T09:57:48Z
date_published: 2020-07-08T00:00:00Z
date_updated: 2024-03-04T10:02:49Z
day: '08'
department:
- _id: JoFi
doi: 10.23919/eucap48036.2020.9135962
language:
- iso: eng
month: '07'
oa_version: None
publication: 14th European Conference on Antennas and Propagation
publication_identifier:
eisbn:
- '9788831299008'
publication_status: published
publisher: IEEE
quality_controlled: '1'
status: public
title: Compact millimeter and submillimeter-wave photonic radiometer for cubesats
type: conference
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2020'
...
---
_id: '15074'
abstract:
- lang: eng
text: We introduce a new graph problem, the token dropping game, and we show how
to solve it efficiently in a distributed setting. We use the token dropping game
as a tool to design an efficient distributed algorithm for the stable orientation
problem, which is a special case of the more general locally optimal semi-matching
problem. The prior work by Czygrinow et al. (DISC 2012) finds a locally optimal
semi-matching in O(Δ⁵) rounds in graphs of maximum degree Δ, which directly implies
an algorithm with the same runtime for stable orientations. We improve the runtime
to O(Δ⁴) for stable orientations and prove a lower bound of Ω(Δ) rounds.
alternative_title:
- LIPIcs
article_number: '40'
article_processing_charge: No
author:
- first_name: Sebastian
full_name: Brandt, Sebastian
last_name: Brandt
- first_name: Barbara
full_name: Keller, Barbara
last_name: Keller
- first_name: Joel
full_name: Rybicki, Joel
id: 334EFD2E-F248-11E8-B48F-1D18A9856A87
last_name: Rybicki
orcid: 0000-0002-6432-6646
- first_name: Jukka
full_name: Suomela, Jukka
last_name: Suomela
- first_name: Jara
full_name: Uitto, Jara
last_name: Uitto
citation:
ama: 'Brandt S, Keller B, Rybicki J, Suomela J, Uitto J. Brief announcement: Efficient
load-balancing through distributed token dropping. In: 34th International Symposium
on Distributed Computing. Vol 179. Schloss Dagstuhl - Leibniz-Zentrum für
Informatik; 2020. doi:10.4230/LIPIcs.DISC.2020.40'
apa: 'Brandt, S., Keller, B., Rybicki, J., Suomela, J., & Uitto, J. (2020).
Brief announcement: Efficient load-balancing through distributed token dropping.
In 34th International Symposium on Distributed Computing (Vol. 179). Virtual:
Schloss Dagstuhl - Leibniz-Zentrum für Informatik. https://doi.org/10.4230/LIPIcs.DISC.2020.40'
chicago: 'Brandt, Sebastian, Barbara Keller, Joel Rybicki, Jukka Suomela, and Jara
Uitto. “Brief Announcement: Efficient Load-Balancing through Distributed Token
Dropping.” In 34th International Symposium on Distributed Computing, Vol.
179. Schloss Dagstuhl - Leibniz-Zentrum für Informatik, 2020. https://doi.org/10.4230/LIPIcs.DISC.2020.40.'
ieee: 'S. Brandt, B. Keller, J. Rybicki, J. Suomela, and J. Uitto, “Brief announcement:
Efficient load-balancing through distributed token dropping,” in 34th International
Symposium on Distributed Computing, Virtual, 2020, vol. 179.'
ista: 'Brandt S, Keller B, Rybicki J, Suomela J, Uitto J. 2020. Brief announcement:
Efficient load-balancing through distributed token dropping. 34th International
Symposium on Distributed Computing. DISC: Symposium on Distributed Computing,
LIPIcs, vol. 179, 40.'
mla: 'Brandt, Sebastian, et al. “Brief Announcement: Efficient Load-Balancing through
Distributed Token Dropping.” 34th International Symposium on Distributed Computing,
vol. 179, 40, Schloss Dagstuhl - Leibniz-Zentrum für Informatik, 2020, doi:10.4230/LIPIcs.DISC.2020.40.'
short: S. Brandt, B. Keller, J. Rybicki, J. Suomela, J. Uitto, in:, 34th International
Symposium on Distributed Computing, Schloss Dagstuhl - Leibniz-Zentrum für Informatik,
2020.
conference:
end_date: 2020-10-16
location: Virtual
name: 'DISC: Symposium on Distributed Computing'
start_date: 2020-10-12
date_created: 2024-03-05T07:09:12Z
date_published: 2020-10-07T00:00:00Z
date_updated: 2024-03-05T07:13:13Z
day: '07'
ddc:
- '000'
department:
- _id: DaAl
doi: 10.4230/LIPIcs.DISC.2020.40
external_id:
arxiv:
- '2005.07761'
file:
- access_level: open_access
checksum: 23e2d9321aef53092dc1e24a8ab82d72
content_type: application/pdf
creator: dernst
date_created: 2024-03-05T07:08:27Z
date_updated: 2024-03-05T07:08:27Z
file_id: '15075'
file_name: 2020_LIPIcs_Brandt.pdf
file_size: 303529
relation: main_file
success: 1
file_date_updated: 2024-03-05T07:08:27Z
has_accepted_license: '1'
intvolume: ' 179'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/3.0/
month: '10'
oa: 1
oa_version: Published Version
publication: 34th International Symposium on Distributed Computing
publication_status: published
publisher: Schloss Dagstuhl - Leibniz-Zentrum für Informatik
quality_controlled: '1'
related_material:
record:
- id: '9678'
relation: later_version
status: public
scopus_import: '1'
status: public
title: 'Brief announcement: Efficient load-balancing through distributed token dropping'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/3.0/legalcode
name: Creative Commons Attribution 3.0 Unported (CC BY 3.0)
short: CC BY (3.0)
type: conference
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 179
year: '2020'
...
---
_id: '15077'
abstract:
- lang: eng
text: "We consider the following dynamic load-balancing process: given an underlying
graph G with n nodes, in each step t≥ 0, one unit of load is created, and placed
at a randomly chosen graph node. In the same step, the chosen node picks a random
neighbor, and the two nodes balance their loads by averaging them. We are interested
in the expected gap between the minimum and maximum loads at nodes as the process
progresses, and its dependence on n and on the graph structure. Variants of the
above graphical balanced allocation process have been studied previously by Peres,
Talwar, and Wieder [Peres et al., 2015], and by Sauerwald and Sun [Sauerwald and
Sun, 2015]. These authors left as open the question of characterizing the gap
in the case of cycle graphs in the dynamic case, where weights are created during
the algorithm’s execution. For this case, the only known upper bound is of \U0001D4AA(n
log n), following from a majorization argument due to [Peres et al., 2015], which
analyzes a related graphical allocation process. In this paper, we provide an
upper bound of \U0001D4AA (√n log n) on the expected gap of the above process
for cycles of length n. We introduce a new potential analysis technique, which
enables us to bound the difference in load between k-hop neighbors on the cycle,
for any k ≤ n/2. We complement this with a \"gap covering\" argument, which bounds
the maximum value of the gap by bounding its value across all possible subsets
of a certain structure, and recursively bounding the gaps within each subset.
We provide analytical and experimental evidence that our upper bound on the gap
is tight up to a logarithmic factor."
acknowledgement: "The authors sincerely thank Thomas Sauerwald and George Giakkoupis
for insightful discussions, and Mohsen Ghaffari, Yuval Peres, and Udi Wieder for
feedback on earlier\r\nversions of this draft. We also thank the ICALP anonymous
reviewers for their very useful comments.\r\nFunding: European Research Council
funding award PR1042ERC01"
alternative_title:
- LIPIcs
article_number: '7'
article_processing_charge: No
author:
- first_name: Dan-Adrian
full_name: Alistarh, Dan-Adrian
id: 4A899BFC-F248-11E8-B48F-1D18A9856A87
last_name: Alistarh
orcid: 0000-0003-3650-940X
- first_name: Giorgi
full_name: Nadiradze, Giorgi
id: 3279A00C-F248-11E8-B48F-1D18A9856A87
last_name: Nadiradze
orcid: 0000-0001-5634-0731
- first_name: Amirmojtaba
full_name: Sabour, Amirmojtaba
id: bcc145fd-e77f-11ea-ae8b-80d661dbff67
last_name: Sabour
citation:
ama: 'Alistarh D-A, Nadiradze G, Sabour A. Dynamic averaging load balancing on cycles.
In: 47th International Colloquium on Automata, Languages, and Programming.
Vol 168. Schloss Dagstuhl - Leibniz-Zentrum für Informatik; 2020. doi:10.4230/LIPIcs.ICALP.2020.7'
apa: 'Alistarh, D.-A., Nadiradze, G., & Sabour, A. (2020). Dynamic averaging
load balancing on cycles. In 47th International Colloquium on Automata, Languages,
and Programming (Vol. 168). Saarbrücken, Germany, Virtual: Schloss Dagstuhl
- Leibniz-Zentrum für Informatik. https://doi.org/10.4230/LIPIcs.ICALP.2020.7'
chicago: Alistarh, Dan-Adrian, Giorgi Nadiradze, and Amirmojtaba Sabour. “Dynamic
Averaging Load Balancing on Cycles.” In 47th International Colloquium on Automata,
Languages, and Programming, Vol. 168. Schloss Dagstuhl - Leibniz-Zentrum für
Informatik, 2020. https://doi.org/10.4230/LIPIcs.ICALP.2020.7.
ieee: D.-A. Alistarh, G. Nadiradze, and A. Sabour, “Dynamic averaging load balancing
on cycles,” in 47th International Colloquium on Automata, Languages, and Programming,
Saarbrücken, Germany, Virtual, 2020, vol. 168.
ista: 'Alistarh D-A, Nadiradze G, Sabour A. 2020. Dynamic averaging load balancing
on cycles. 47th International Colloquium on Automata, Languages, and Programming.
ICALP: International Colloquium on Automata, Languages, and Programming, LIPIcs,
vol. 168, 7.'
mla: Alistarh, Dan-Adrian, et al. “Dynamic Averaging Load Balancing on Cycles.”
47th International Colloquium on Automata, Languages, and Programming,
vol. 168, 7, Schloss Dagstuhl - Leibniz-Zentrum für Informatik, 2020, doi:10.4230/LIPIcs.ICALP.2020.7.
short: D.-A. Alistarh, G. Nadiradze, A. Sabour, in:, 47th International Colloquium
on Automata, Languages, and Programming, Schloss Dagstuhl - Leibniz-Zentrum für
Informatik, 2020.
conference:
end_date: 2020-07-11
location: Saarbrücken, Germany, Virtual
name: 'ICALP: International Colloquium on Automata, Languages, and Programming'
start_date: 2020-07-08
date_created: 2024-03-05T07:25:37Z
date_published: 2020-06-29T00:00:00Z
date_updated: 2024-03-05T07:35:53Z
day: '29'
ddc:
- '000'
department:
- _id: DaAl
doi: 10.4230/LIPIcs.ICALP.2020.7
ec_funded: 1
external_id:
arxiv:
- '2003.09297'
file:
- access_level: open_access
checksum: e5eb16199f4ccfd77a321977eb3f026f
content_type: application/pdf
creator: dernst
date_created: 2024-03-05T07:25:15Z
date_updated: 2024-03-05T07:25:15Z
file_id: '15078'
file_name: 2020_LIPIcs_Alistarh.pdf
file_size: 782987
relation: main_file
success: 1
file_date_updated: 2024-03-05T07:25:15Z
has_accepted_license: '1'
intvolume: ' 168'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
project:
- _id: 268A44D6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '805223'
name: Elastic Coordination for Scalable Machine Learning
publication: 47th International Colloquium on Automata, Languages, and Programming
publication_status: published
publisher: Schloss Dagstuhl - Leibniz-Zentrum für Informatik
quality_controlled: '1'
related_material:
record:
- id: '8286'
relation: later_version
status: public
scopus_import: '1'
status: public
title: Dynamic averaging load balancing on cycles
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/3.0/legalcode
name: Creative Commons Attribution 3.0 Unported (CC BY 3.0)
short: CC BY (3.0)
type: conference
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 168
year: '2020'
...
---
_id: '15082'
abstract:
- lang: eng
text: "Two plane drawings of geometric graphs on the same set of points are called
disjoint compatible if their union is plane and they do not have an edge in common.
For a given set S of 2n points two plane drawings of perfect matchings M1 and
M2 (which do not need to be disjoint nor compatible) are disjoint tree-compatible
if there exists a plane drawing of a spanning tree T on S which is disjoint compatible
to both M1 and M2.\r\nWe show that the graph of all disjoint tree-compatible perfect
geometric matchings on 2n points in convex position is connected if and only if
2n ≥ 10. Moreover, in that case the diameter\r\nof this graph is either 4 or 5,
independent of n."
acknowledgement: Research on this work was initiated at the 6th Austrian-Japanese-Mexican-Spanish
Workshop on Discrete Geometry and continued during the 16th European Geometric Graph-Week,
both held near Strobl, Austria. We are grateful to the participants for the inspiring
atmosphere. We especially thank Alexander Pilz for bringing this class of problems
to our attention and Birgit Vogtenhuber for inspiring discussions. D.P. is partially
supported by the FWF grant I 3340-N35 (Collaborative DACH project Arrangements and
Drawings). The research stay of P.P. at IST Austria is funded by the project CZ.02.2.69/0.0/0.0/17_050/0008466
Improvement of internationalization in the field of research and development at
Charles University, through the support of quality projects MSCA-IF. This project
has received funding from the European Union’s Horizon 2020 research and innovation
programme under the Marie Skłodowska-Curie grant agreement No 734922.
article_number: '56'
article_processing_charge: No
author:
- first_name: Oswin
full_name: Aichholzer, Oswin
last_name: Aichholzer
- first_name: Julia
full_name: Obmann, Julia
last_name: Obmann
- first_name: Pavel
full_name: Patak, Pavel
id: B593B804-1035-11EA-B4F1-947645A5BB83
last_name: Patak
- first_name: Daniel
full_name: Perz, Daniel
last_name: Perz
- first_name: Josef
full_name: Tkadlec, Josef
id: 3F24CCC8-F248-11E8-B48F-1D18A9856A87
last_name: Tkadlec
orcid: 0000-0002-1097-9684
citation:
ama: 'Aichholzer O, Obmann J, Patak P, Perz D, Tkadlec J. Disjoint tree-compatible
plane perfect matchings. In: 36th European Workshop on Computational Geometry.
; 2020.'
apa: Aichholzer, O., Obmann, J., Patak, P., Perz, D., & Tkadlec, J. (2020).
Disjoint tree-compatible plane perfect matchings. In 36th European Workshop
on Computational Geometry. Würzburg, Germany, Virtual.
chicago: Aichholzer, Oswin, Julia Obmann, Pavel Patak, Daniel Perz, and Josef Tkadlec.
“Disjoint Tree-Compatible Plane Perfect Matchings.” In 36th European Workshop
on Computational Geometry, 2020.
ieee: O. Aichholzer, J. Obmann, P. Patak, D. Perz, and J. Tkadlec, “Disjoint tree-compatible
plane perfect matchings,” in 36th European Workshop on Computational Geometry,
Würzburg, Germany, Virtual, 2020.
ista: 'Aichholzer O, Obmann J, Patak P, Perz D, Tkadlec J. 2020. Disjoint tree-compatible
plane perfect matchings. 36th European Workshop on Computational Geometry. EuroCG:
European Workshop on Computational Geometry, 56.'
mla: Aichholzer, Oswin, et al. “Disjoint Tree-Compatible Plane Perfect Matchings.”
36th European Workshop on Computational Geometry, 56, 2020.
short: O. Aichholzer, J. Obmann, P. Patak, D. Perz, J. Tkadlec, in:, 36th European
Workshop on Computational Geometry, 2020.
conference:
end_date: 2020-03-18
location: Würzburg, Germany, Virtual
name: 'EuroCG: European Workshop on Computational Geometry'
start_date: 2020-03-16
date_created: 2024-03-05T08:57:17Z
date_published: 2020-04-01T00:00:00Z
date_updated: 2024-03-05T09:00:07Z
day: '01'
department:
- _id: KrCh
- _id: UlWa
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www1.pub.informatik.uni-wuerzburg.de/eurocg2020/data/uploads/papers/eurocg20_paper_56.pdf
month: '04'
oa: 1
oa_version: Published Version
publication: 36th European Workshop on Computational Geometry
publication_status: published
quality_controlled: '1'
status: public
title: Disjoint tree-compatible plane perfect matchings
type: conference
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2020'
...
---
_id: '6748'
abstract:
- lang: eng
text: "Fitting a function by using linear combinations of a large number N of `simple'
components is one of the most fruitful ideas in statistical learning. This idea
lies at the core of a variety of methods, from two-layer neural networks to kernel
regression, to boosting. In general, the resulting risk minimization problem is
non-convex and is solved by gradient descent or its variants. Unfortunately, little
is known about global convergence properties of these approaches.\r\nHere we consider
the problem of learning a concave function f on a compact convex domain Ω⊆ℝd,
using linear combinations of `bump-like' components (neurons). The parameters
to be fitted are the centers of N bumps, and the resulting empirical risk minimization
problem is highly non-convex. We prove that, in the limit in which the number
of neurons diverges, the evolution of gradient descent converges to a Wasserstein
gradient flow in the space of probability distributions over Ω. Further, when
the bump width δ tends to 0, this gradient flow has a limit which is a viscous
porous medium equation. Remarkably, the cost function optimized by this gradient
flow exhibits a special property known as displacement convexity, which implies
exponential convergence rates for N→∞, δ→0. Surprisingly, this asymptotic theory
appears to capture well the behavior for moderate values of δ,N. Explaining this
phenomenon, and understanding the dependence on δ,N in a quantitative manner remains
an outstanding challenge."
article_processing_charge: No
article_type: original
author:
- first_name: Adel
full_name: Javanmard, Adel
last_name: Javanmard
- first_name: Marco
full_name: Mondelli, Marco
id: 27EB676C-8706-11E9-9510-7717E6697425
last_name: Mondelli
orcid: 0000-0002-3242-7020
- first_name: Andrea
full_name: Montanari, Andrea
last_name: Montanari
citation:
ama: Javanmard A, Mondelli M, Montanari A. Analysis of a two-layer neural network
via displacement convexity. Annals of Statistics. 2020;48(6):3619-3642.
doi:10.1214/20-AOS1945
apa: Javanmard, A., Mondelli, M., & Montanari, A. (2020). Analysis of a two-layer
neural network via displacement convexity. Annals of Statistics. Institute
of Mathematical Statistics. https://doi.org/10.1214/20-AOS1945
chicago: Javanmard, Adel, Marco Mondelli, and Andrea Montanari. “Analysis of a Two-Layer
Neural Network via Displacement Convexity.” Annals of Statistics. Institute
of Mathematical Statistics, 2020. https://doi.org/10.1214/20-AOS1945.
ieee: A. Javanmard, M. Mondelli, and A. Montanari, “Analysis of a two-layer neural
network via displacement convexity,” Annals of Statistics, vol. 48, no.
6. Institute of Mathematical Statistics, pp. 3619–3642, 2020.
ista: Javanmard A, Mondelli M, Montanari A. 2020. Analysis of a two-layer neural
network via displacement convexity. Annals of Statistics. 48(6), 3619–3642.
mla: Javanmard, Adel, et al. “Analysis of a Two-Layer Neural Network via Displacement
Convexity.” Annals of Statistics, vol. 48, no. 6, Institute of Mathematical
Statistics, 2020, pp. 3619–42, doi:10.1214/20-AOS1945.
short: A. Javanmard, M. Mondelli, A. Montanari, Annals of Statistics 48 (2020) 3619–3642.
date_created: 2019-07-31T09:39:42Z
date_published: 2020-12-11T00:00:00Z
date_updated: 2024-03-06T08:28:50Z
day: '11'
department:
- _id: MaMo
doi: 10.1214/20-AOS1945
external_id:
arxiv:
- '1901.01375'
isi:
- '000598369200021'
intvolume: ' 48'
isi: 1
issue: '6'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://arxiv.org/abs/1901.01375
month: '12'
oa: 1
oa_version: Preprint
page: 3619-3642
publication: Annals of Statistics
publication_identifier:
eissn:
- 1941-7330
issn:
- 1932-6157
publication_status: published
publisher: Institute of Mathematical Statistics
quality_controlled: '1'
status: public
title: Analysis of a two-layer neural network via displacement convexity
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 48
year: '2020'
...
---
_id: '15070'
abstract:
- lang: eng
text: This workshop focused on interactions between the various perspectives on
the moduli space of Higgs bundles over a Riemann surface. This subject draws on
algebraic geometry, geometric topology, geometric analysis and mathematical physics,
and the goal was to promote interactions between these various branches of the
subject. The main current directions of research were well represented by the
participants, and the talks included many from both senior and junior participants.
article_processing_charge: No
article_type: original
author:
- first_name: Lara
full_name: Anderson, Lara
last_name: Anderson
- first_name: Tamás
full_name: Hausel, Tamás
id: 4A0666D8-F248-11E8-B48F-1D18A9856A87
last_name: Hausel
- first_name: Rafe
full_name: Mazzeo, Rafe
last_name: Mazzeo
- first_name: Laura
full_name: Schaposnik, Laura
last_name: Schaposnik
citation:
ama: Anderson L, Hausel T, Mazzeo R, Schaposnik L. Geometry and physics of Higgs
bundles. Oberwolfach Reports. 2020;16(2):1357-1417. doi:10.4171/owr/2019/23
apa: Anderson, L., Hausel, T., Mazzeo, R., & Schaposnik, L. (2020). Geometry
and physics of Higgs bundles. Oberwolfach Reports. European Mathematical
Society. https://doi.org/10.4171/owr/2019/23
chicago: Anderson, Lara, Tamás Hausel, Rafe Mazzeo, and Laura Schaposnik. “Geometry
and Physics of Higgs Bundles.” Oberwolfach Reports. European Mathematical
Society, 2020. https://doi.org/10.4171/owr/2019/23.
ieee: L. Anderson, T. Hausel, R. Mazzeo, and L. Schaposnik, “Geometry and physics
of Higgs bundles,” Oberwolfach Reports, vol. 16, no. 2. European Mathematical
Society, pp. 1357–1417, 2020.
ista: Anderson L, Hausel T, Mazzeo R, Schaposnik L. 2020. Geometry and physics of
Higgs bundles. Oberwolfach Reports. 16(2), 1357–1417.
mla: Anderson, Lara, et al. “Geometry and Physics of Higgs Bundles.” Oberwolfach
Reports, vol. 16, no. 2, European Mathematical Society, 2020, pp. 1357–417,
doi:10.4171/owr/2019/23.
short: L. Anderson, T. Hausel, R. Mazzeo, L. Schaposnik, Oberwolfach Reports 16
(2020) 1357–1417.
date_created: 2024-03-04T11:36:31Z
date_published: 2020-06-04T00:00:00Z
date_updated: 2024-03-11T09:20:34Z
day: '04'
department:
- _id: TaHa
doi: 10.4171/owr/2019/23
intvolume: ' 16'
issue: '2'
keyword:
- Organic Chemistry
- Biochemistry
language:
- iso: eng
month: '06'
oa_version: None
page: 1357-1417
publication: Oberwolfach Reports
publication_identifier:
issn:
- 1660-8933
publication_status: published
publisher: European Mathematical Society
quality_controlled: '1'
status: public
title: Geometry and physics of Higgs bundles
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 16
year: '2020'
...
---
_id: '8741'
abstract:
- lang: eng
text: "In ecology, climate and other fields, (sub)systems have been identified that
can transition into a qualitatively different state when a critical threshold
or tipping point in a driving process is crossed. An understanding of those tipping
elements is of great interest given the increasing influence of humans on the
biophysical Earth system. Complex interactions exist between tipping elements,
e.g. physical mechanisms connect subsystems of the climate system. Based on earlier
work on such coupled nonlinear systems, we systematically assessed the qualitative
long-term behaviour of interacting tipping elements. We developed an understanding
of the consequences of interactions\r\non the tipping behaviour allowing for tipping
cascades to emerge under certain conditions. The (narrative) application of\r\nthese
qualitative results to real-world examples of interacting tipping elements indicates
that tipping cascades with profound consequences may occur: the interacting Greenland
ice sheet and thermohaline ocean circulation might tip before the tipping points
of the isolated subsystems are crossed. The eutrophication of the first lake in
a lake chain might propagate through the following lakes without a crossing of
their individual critical nutrient input levels. The possibility of emerging cascading
tipping dynamics calls for the development of a unified theory of interacting
tipping elements and the quantitative analysis of interacting real-world tipping
elements."
acknowledgement: "V.K. thanks the German National Academic Foundation (Studienstiftung
des deutschen Volkes) for financial\r\nsupport. J.F.D. is grateful for financial
support by the Stordalen Foundation via the Planetary Boundary Research\r\nNetwork
(PB.net), the Earth League’s EarthDoc program and the European Research Council
Advanced Grant\r\nproject ERA (Earth Resilience in the Anthropocene). We are thankful
for support by the Leibniz Association\r\n(project DominoES).\r\nAcknowledgements.
This work has been performed in the context of the copan collaboration and the FutureLab
on Earth\r\nResilience in the Anthropocene at the Potsdam Institute for Climate
Impact Research. Furthermore, we acknowledge\r\ndiscussions with and helpful comments
by N. Wunderling, J. Heitzig and M. Wiedermann."
article_number: '200599'
article_processing_charge: No
article_type: original
author:
- first_name: Ann Kristin
full_name: Klose, Ann Kristin
last_name: Klose
- first_name: Volker
full_name: Karle, Volker
id: D7C012AE-D7ED-11E9-95E8-1EC5E5697425
last_name: Karle
orcid: 0000-0002-6963-0129
- first_name: Ricarda
full_name: Winkelmann, Ricarda
last_name: Winkelmann
- first_name: Jonathan F.
full_name: Donges, Jonathan F.
last_name: Donges
citation:
ama: 'Klose AK, Karle V, Winkelmann R, Donges JF. Emergence of cascading dynamics
in interacting tipping elements of ecology and climate: Cascading dynamics in
tipping elements. Royal Society Open Science. 2020;7(6). doi:10.1098/rsos.200599'
apa: 'Klose, A. K., Karle, V., Winkelmann, R., & Donges, J. F. (2020). Emergence
of cascading dynamics in interacting tipping elements of ecology and climate:
Cascading dynamics in tipping elements. Royal Society Open Science. The
Royal Society. https://doi.org/10.1098/rsos.200599'
chicago: 'Klose, Ann Kristin, Volker Karle, Ricarda Winkelmann, and Jonathan F.
Donges. “Emergence of Cascading Dynamics in Interacting Tipping Elements of Ecology
and Climate: Cascading Dynamics in Tipping Elements.” Royal Society Open Science.
The Royal Society, 2020. https://doi.org/10.1098/rsos.200599.'
ieee: 'A. K. Klose, V. Karle, R. Winkelmann, and J. F. Donges, “Emergence of cascading
dynamics in interacting tipping elements of ecology and climate: Cascading dynamics
in tipping elements,” Royal Society Open Science, vol. 7, no. 6. The Royal
Society, 2020.'
ista: 'Klose AK, Karle V, Winkelmann R, Donges JF. 2020. Emergence of cascading
dynamics in interacting tipping elements of ecology and climate: Cascading dynamics
in tipping elements. Royal Society Open Science. 7(6), 200599.'
mla: 'Klose, Ann Kristin, et al. “Emergence of Cascading Dynamics in Interacting
Tipping Elements of Ecology and Climate: Cascading Dynamics in Tipping Elements.”
Royal Society Open Science, vol. 7, no. 6, 200599, The Royal Society, 2020,
doi:10.1098/rsos.200599.'
short: A.K. Klose, V. Karle, R. Winkelmann, J.F. Donges, Royal Society Open Science
7 (2020).
date_created: 2020-11-08T23:01:25Z
date_published: 2020-06-01T00:00:00Z
date_updated: 2024-03-12T12:31:30Z
day: '01'
ddc:
- '530'
- '550'
department:
- _id: MiLe
doi: 10.1098/rsos.200599
external_id:
arxiv:
- '1910.12042'
isi:
- '000545625200001'
file:
- access_level: open_access
checksum: 5505c445de373bfd836eb4d3b48b1f37
content_type: application/pdf
creator: dernst
date_created: 2020-11-09T09:07:11Z
date_updated: 2020-11-09T09:07:11Z
file_id: '8748'
file_name: 2020_RoyalSocOpenScience_Klose.pdf
file_size: 1611485
relation: main_file
success: 1
file_date_updated: 2020-11-09T09:07:11Z
has_accepted_license: '1'
intvolume: ' 7'
isi: 1
issue: '6'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
publication: Royal Society Open Science
publication_identifier:
eissn:
- '20545703'
publication_status: published
publisher: The Royal Society
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Emergence of cascading dynamics in interacting tipping elements of ecology
and climate: Cascading dynamics in tipping elements'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 7
year: '2020'
...
---
_id: '7687'
abstract:
- lang: eng
text: A working group, which was established within the Network of Repository Managers (RepManNet), has dealt with common certifications for repositories. In
addition, current requirements of the research funding agencies FWF and EU were
also taken into account. The Core Trust Seal was examined in more detail. For
this purpose, a questionnaire was sent to those organizations that are already certified
with CTS in Austria. The answers were summarized and evaluated anonymously. It
is recommended to go for a repository certification. Moreover, the development
of a DINI certificate in Austria is strongly suggested.
- lang: ger
text: ' Eine Arbeitsgruppe, die im Rahmen des Netzwerks für RepositorienmanagerInnen
(RepManNet) entstanden ist, hat sich mit gängigen Zertifizierungen für Repositorien
beschäftigt. Weiters wurden aktuelle Vorgaben der Forschungsförderer FWF und EU
herangezogen. Das Core Trust Seal wurde genauer betrachtet. Hierfür wurden jenen Organisationen, die in Österreich bereits mit CTS zertifiziert
sind, ein Fragebogen übermittelt. Die Antworten wurden anonymisiert zusammengefasst
und ausgewertet. Plädiert wird für eine Zertifizierung von Repositorien und die
Entwicklung einer DINI-Zertifizierung in Österreich.'
article_processing_charge: No
article_type: original
author:
- first_name: Doris
full_name: Ernst, Doris
id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
last_name: Ernst
orcid: 0000-0002-2354-0195
- first_name: Gertraud
full_name: Novotny, Gertraud
last_name: Novotny
- first_name: Eva Maria
full_name: Schönher, Eva Maria
last_name: Schönher
citation:
ama: Ernst D, Novotny G, Schönher EM. (Core Trust) Seal your repository! Mitteilungen
der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare. 2020;73(1):46-59.
doi:10.31263/voebm.v73i1.3491
apa: Ernst, D., Novotny, G., & Schönher, E. M. (2020). (Core Trust) Seal your
repository! Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen
und Bibliothekare. Vereinigung Osterreichischer Bibliothekarinnen und Bibliothekare.
https://doi.org/10.31263/voebm.v73i1.3491
chicago: Ernst, Doris, Gertraud Novotny, and Eva Maria Schönher. “(Core Trust) Seal
your repository!” Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen
und Bibliothekare. Vereinigung Osterreichischer Bibliothekarinnen und Bibliothekare,
2020. https://doi.org/10.31263/voebm.v73i1.3491.
ieee: D. Ernst, G. Novotny, and E. M. Schönher, “(Core Trust) Seal your repository!,”
Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare,
vol. 73, no. 1. Vereinigung Osterreichischer Bibliothekarinnen und Bibliothekare,
pp. 46–59, 2020.
ista: Ernst D, Novotny G, Schönher EM. 2020. (Core Trust) Seal your repository!
Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare.
73(1), 46–59.
mla: Ernst, Doris, et al. “(Core Trust) Seal your repository!” Mitteilungen der
Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare, vol. 73,
no. 1, Vereinigung Osterreichischer Bibliothekarinnen und Bibliothekare, 2020,
pp. 46–59, doi:10.31263/voebm.v73i1.3491.
short: D. Ernst, G. Novotny, E.M. Schönher, Mitteilungen der Vereinigung Österreichischer
Bibliothekarinnen und Bibliothekare 73 (2020) 46–59.
date_created: 2020-04-28T08:37:38Z
date_published: 2020-04-28T00:00:00Z
date_updated: 2024-03-12T10:12:33Z
day: '28'
ddc:
- '020'
department:
- _id: E-Lib
doi: 10.31263/voebm.v73i1.3491
file:
- access_level: open_access
checksum: fee784f15a489deb7def6ccf8c5bf8c3
content_type: application/pdf
creator: dernst
date_created: 2020-06-17T10:50:13Z
date_updated: 2024-03-12T10:12:33Z
file_id: '7970'
file_name: 2020_VOEB_Ernst.pdf
file_size: 579291
relation: main_file
file_date_updated: 2024-03-12T10:12:33Z
has_accepted_license: '1'
intvolume: ' 73'
issue: '1'
language:
- iso: ger
month: '04'
oa: 1
oa_version: Published Version
page: 46-59
popular_science: '1'
publication: Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare
publication_identifier:
issn:
- 1022-2588
publication_status: published
publisher: Vereinigung Osterreichischer Bibliothekarinnen und Bibliothekare
scopus_import: '1'
status: public
title: (Core Trust) Seal your repository!
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 73
year: '2020'
...
---
_id: '15079'
abstract:
- lang: eng
text: "Large complex systems tend to develop universal patterns that often represent
their essential characteristics. For example, the cumulative effects of independent
or weakly dependent random variables often yield the Gaussian universality class
via the central limit theorem. For non-commutative random variables, e.g. matrices,
the Gaussian behavior is often replaced by another universality class, commonly
called random matrix statistics. Nearby eigenvalues are strongly correlated, and,
remarkably, their correlation structure is universal, depending only on the symmetry
type of the matrix. Even more surprisingly, this feature is not restricted to
matrices; in fact Eugene Wigner, the pioneer of the field, discovered in the 1950s
that distributions of the gaps between energy levels of complicated quantum systems
universally follow the same random matrix statistics. This claim has never been
rigorously proved for any realistic physical system but experimental data and
extensive numerics leave no doubt as to its correctness. Since then random matrices
have proved to be extremely useful phenomenological models in a wide range of
applications beyond quantum physics that include number theory, statistics, neuroscience,
population dynamics, wireless communication and mathematical finance. The ubiquity
of random matrices in natural sciences is still a mystery, but recent years have
witnessed a breakthrough in the mathematical description of the statistical structure
of their spectrum. Random matrices and closely related areas such as log-gases
have become an extremely active research area in probability theory.\r\nThis workshop
brought together outstanding researchers from a variety of mathematical backgrounds
whose areas of research are linked to random matrices. While there are strong
links between their motivations, the techniques used by these researchers span
a large swath of mathematics, ranging from purely algebraic techniques to stochastic
analysis, classical probability theory, operator algebra, supersymmetry, orthogonal
polynomials, etc."
article_processing_charge: No
article_type: original
author:
- first_name: László
full_name: Erdös, László
id: 4DBD5372-F248-11E8-B48F-1D18A9856A87
last_name: Erdös
orcid: 0000-0001-5366-9603
- first_name: Friedrich
full_name: Götze, Friedrich
last_name: Götze
- first_name: Alice
full_name: Guionnet, Alice
last_name: Guionnet
citation:
ama: Erdös L, Götze F, Guionnet A. Random matrices. Oberwolfach Reports.
2020;16(4):3459-3527. doi:10.4171/owr/2019/56
apa: Erdös, L., Götze, F., & Guionnet, A. (2020). Random matrices. Oberwolfach
Reports. European Mathematical Society. https://doi.org/10.4171/owr/2019/56
chicago: Erdös, László, Friedrich Götze, and Alice Guionnet. “Random Matrices.”
Oberwolfach Reports. European Mathematical Society, 2020. https://doi.org/10.4171/owr/2019/56.
ieee: L. Erdös, F. Götze, and A. Guionnet, “Random matrices,” Oberwolfach Reports,
vol. 16, no. 4. European Mathematical Society, pp. 3459–3527, 2020.
ista: Erdös L, Götze F, Guionnet A. 2020. Random matrices. Oberwolfach Reports.
16(4), 3459–3527.
mla: Erdös, László, et al. “Random Matrices.” Oberwolfach Reports, vol. 16,
no. 4, European Mathematical Society, 2020, pp. 3459–527, doi:10.4171/owr/2019/56.
short: L. Erdös, F. Götze, A. Guionnet, Oberwolfach Reports 16 (2020) 3459–3527.
date_created: 2024-03-05T07:54:44Z
date_published: 2020-11-19T00:00:00Z
date_updated: 2024-03-12T12:25:18Z
day: '19'
department:
- _id: LaEr
doi: 10.4171/owr/2019/56
intvolume: ' 16'
issue: '4'
language:
- iso: eng
month: '11'
oa_version: None
page: 3459-3527
publication: Oberwolfach Reports
publication_identifier:
issn:
- 1660-8933
publication_status: published
publisher: European Mathematical Society
quality_controlled: '1'
status: public
title: Random matrices
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 16
year: '2020'
...
---
_id: '15072'
abstract:
- lang: eng
text: The interaction among fundamental particles in nature leads to many interesting
effects in quantum statistical mechanics; examples include superconductivity for
charged systems and superfluidity in cold gases. It is a huge challenge for mathematical
physics to understand the collective behavior of systems containing a large number
of particles, emerging from known microscopic interactions. In this workshop we
brought together researchers working on different aspects of many-body quantum
mechanics to discuss recent developments, exchange ideas and propose new challenges
and research directions.
article_processing_charge: No
article_type: original
author:
- first_name: Christian
full_name: Hainzl, Christian
last_name: Hainzl
- first_name: Benjamin
full_name: Schlein, Benjamin
last_name: Schlein
- first_name: Robert
full_name: Seiringer, Robert
id: 4AFD0470-F248-11E8-B48F-1D18A9856A87
last_name: Seiringer
orcid: 0000-0002-6781-0521
- first_name: Simone
full_name: Warzel, Simone
last_name: Warzel
citation:
ama: Hainzl C, Schlein B, Seiringer R, Warzel S. Many-body quantum systems. Oberwolfach
Reports. 2020;16(3):2541-2603. doi:10.4171/owr/2019/41
apa: Hainzl, C., Schlein, B., Seiringer, R., & Warzel, S. (2020). Many-body
quantum systems. Oberwolfach Reports. European Mathematical Society. https://doi.org/10.4171/owr/2019/41
chicago: Hainzl, Christian, Benjamin Schlein, Robert Seiringer, and Simone Warzel.
“Many-Body Quantum Systems.” Oberwolfach Reports. European Mathematical
Society, 2020. https://doi.org/10.4171/owr/2019/41.
ieee: C. Hainzl, B. Schlein, R. Seiringer, and S. Warzel, “Many-body quantum systems,”
Oberwolfach Reports, vol. 16, no. 3. European Mathematical Society, pp.
2541–2603, 2020.
ista: Hainzl C, Schlein B, Seiringer R, Warzel S. 2020. Many-body quantum systems.
Oberwolfach Reports. 16(3), 2541–2603.
mla: Hainzl, Christian, et al. “Many-Body Quantum Systems.” Oberwolfach Reports,
vol. 16, no. 3, European Mathematical Society, 2020, pp. 2541–603, doi:10.4171/owr/2019/41.
short: C. Hainzl, B. Schlein, R. Seiringer, S. Warzel, Oberwolfach Reports 16 (2020)
2541–2603.
date_created: 2024-03-04T11:46:12Z
date_published: 2020-09-10T00:00:00Z
date_updated: 2024-03-12T12:02:00Z
day: '10'
department:
- _id: RoSe
doi: 10.4171/owr/2019/41
intvolume: ' 16'
issue: '3'
language:
- iso: eng
month: '09'
oa_version: None
page: 2541-2603
publication: Oberwolfach Reports
publication_identifier:
issn:
- 1660-8933
publication_status: published
publisher: European Mathematical Society
quality_controlled: '1'
status: public
title: Many-body quantum systems
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 16
year: '2020'
...
---
_id: '15071'
abstract:
- lang: eng
text: "A mesophilic methanogenic culture, designated JL01, was isolated from Holocene
permafrost in the Russian Arctic [1]. After long-term extensive cultivation at
15°C it turned out to be a tied binary culture of archaeal (JL01) and bacterial
(Sphaerochaeta associata GLS2) strains.\r\nStrain JL01 was a strict anaerobe and
grew on methanol, acetate and methylamines as energy and carbon sources. Cells
were irregular coccoid, non-motile, non-spore-forming, and Gram-stainpositive.
Optimum conditions for growth were 24-28 oC, pH 6.8–7.3 and 0.075-0.1 M NaCl.\r\nPhylogenetic
tree reconstructions based on 16S rRNA and concatenated alignment of broadly\r\nconserved
protein-coding genes revealed its close relation to Methanosarcina mazei S-6\r\nT
(similarity 99.5%). The comparison of whole genomic sequences (ANI) of the isolate
and the type strain of M.mazei was 98.5%, which is higher than the values recommended
for new species. Thus strain JL01 (=VKM B-2370=JCM 31898) represents the first
M. mazei isolated from permanently subzero Arcticsediments. The long-term co-cultivation
of JL01 with S. associata GLS2T showed the methane production without any additional
carbon and energy sources. Genome analysis of S. associata GLS2T revealed putative
genes involved in methanochondroithin catabolism."
acknowledgement: "The work was supported by of Russian Foundation of Basic Research:
grant № 19-04-00831 for Viktoria Shcherbakova and Olga Troshina, grant № 18-34-00334
for Viktoriia Oshurkova and Vladimir Trubitsyn. \r\nWe thank Dr Natalia Suzina (IBPM
RAS, Federal Research Center Pushchino Center for\r\nBiological Research RAS) for
the help with the microscopic studies, respectively; Dr. Margarita Meyer (Division
of Genetics, Department of Medicine, BWH and HMS, USA) and Dr Fedor Kondrashov (IST,
Austria) for their help in obtaining the genomic sequence of strain JL01. "
article_processing_charge: Yes
author:
- first_name: Viktoriia
full_name: Oshurkova, Viktoriia
last_name: Oshurkova
- first_name: Olga
full_name: Troshina, Olga
last_name: Troshina
- first_name: Vladimir
full_name: Trubitsyn, Vladimir
last_name: Trubitsyn
- first_name: Yana
full_name: Ryzhmanova, Yana
last_name: Ryzhmanova
- first_name: Olga
full_name: Bochkareva, Olga
id: C4558D3C-6102-11E9-A62E-F418E6697425
last_name: Bochkareva
orcid: 0000-0003-1006-6639
- first_name: Viktoria
full_name: Shcherbakova, Viktoria
last_name: Shcherbakova
citation:
ama: 'Oshurkova V, Troshina O, Trubitsyn V, Ryzhmanova Y, Bochkareva O, Shcherbakova
V. Characterization of methanosarcina mazei JL01 isolated from holocene arctic
permafrost and study of the archaeon cooperation with bacterium Sphaerochaeta
associata GLS2T. In: Proceedings of 1st International Electronic Conference
on Microbiology. MDPI; 2020. doi:10.3390/ecm2020-07116'
apa: 'Oshurkova, V., Troshina, O., Trubitsyn, V., Ryzhmanova, Y., Bochkareva, O.,
& Shcherbakova, V. (2020). Characterization of methanosarcina mazei JL01 isolated
from holocene arctic permafrost and study of the archaeon cooperation with bacterium
Sphaerochaeta associata GLS2T. In Proceedings of 1st International Electronic
Conference on Microbiology. Virtual: MDPI. https://doi.org/10.3390/ecm2020-07116'
chicago: Oshurkova, Viktoriia, Olga Troshina, Vladimir Trubitsyn, Yana Ryzhmanova,
Olga Bochkareva, and Viktoria Shcherbakova. “Characterization of Methanosarcina
Mazei JL01 Isolated from Holocene Arctic Permafrost and Study of the Archaeon
Cooperation with Bacterium Sphaerochaeta Associata GLS2T.” In Proceedings of
1st International Electronic Conference on Microbiology. MDPI, 2020. https://doi.org/10.3390/ecm2020-07116.
ieee: V. Oshurkova, O. Troshina, V. Trubitsyn, Y. Ryzhmanova, O. Bochkareva, and
V. Shcherbakova, “Characterization of methanosarcina mazei JL01 isolated from
holocene arctic permafrost and study of the archaeon cooperation with bacterium
Sphaerochaeta associata GLS2T,” in Proceedings of 1st International Electronic
Conference on Microbiology, Virtual, 2020.
ista: 'Oshurkova V, Troshina O, Trubitsyn V, Ryzhmanova Y, Bochkareva O, Shcherbakova
V. 2020. Characterization of methanosarcina mazei JL01 isolated from holocene
arctic permafrost and study of the archaeon cooperation with bacterium Sphaerochaeta
associata GLS2T. Proceedings of 1st International Electronic Conference on Microbiology.
ECM: Electronic Conference on Microbiology.'
mla: Oshurkova, Viktoriia, et al. “Characterization of Methanosarcina Mazei JL01
Isolated from Holocene Arctic Permafrost and Study of the Archaeon Cooperation
with Bacterium Sphaerochaeta Associata GLS2T.” Proceedings of 1st International
Electronic Conference on Microbiology, MDPI, 2020, doi:10.3390/ecm2020-07116.
short: V. Oshurkova, O. Troshina, V. Trubitsyn, Y. Ryzhmanova, O. Bochkareva, V.
Shcherbakova, in:, Proceedings of 1st International Electronic Conference on Microbiology,
MDPI, 2020.
conference:
end_date: 2020-11-30
location: Virtual
name: 'ECM: Electronic Conference on Microbiology'
start_date: 2020-11-02
date_created: 2024-03-04T11:41:31Z
date_published: 2020-11-02T00:00:00Z
date_updated: 2024-03-20T08:06:22Z
day: '02'
ddc:
- '570'
department:
- _id: FyKo
doi: 10.3390/ecm2020-07116
file:
- access_level: open_access
checksum: d1914af7811a21a4b2744eb51b5834e3
content_type: application/pdf
creator: dernst
date_created: 2024-03-20T08:05:46Z
date_updated: 2024-03-20T08:05:46Z
file_id: '15127'
file_name: 2020_ECM_Oshurkova.pdf
file_size: 595543
relation: main_file
success: 1
file_date_updated: 2024-03-20T08:05:46Z
has_accepted_license: '1'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
publication: Proceedings of 1st International Electronic Conference on Microbiology
publication_status: published
publisher: MDPI
quality_controlled: '1'
status: public
title: Characterization of methanosarcina mazei JL01 isolated from holocene arctic
permafrost and study of the archaeon cooperation with bacterium Sphaerochaeta associata
GLS2T
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: conference
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2020'
...
---
_id: '15153'
abstract:
- lang: eng
text: Mammalian circadian rhythms are generated by a transcription-based feedback
loop in which CLOCK:BMAL1 drives transcription of its repressors (PER1/2, CRY1/2),
which ultimately interact with CLOCK:BMAL1 to close the feedback loop with ~24
hr periodicity. Here we pinpoint a key difference between CRY1 and CRY2 that underlies
their differential strengths as transcriptional repressors. Both cryptochromes
bind the BMAL1 transactivation domain similarly to sequester it from coactivators
and repress CLOCK:BMAL1 activity. However, we find that CRY1 is recruited with
much higher affinity to the PAS domain core of CLOCK:BMAL1, allowing it to serve
as a stronger repressor that lengthens circadian period. We discovered a dynamic
serine-rich loop adjacent to the secondary pocket in the photolyase homology region
(PHR) domain that regulates differential binding of cryptochromes to the PAS domain
core of CLOCK:BMAL1. Notably, binding of the co-repressor PER2 remodels the serine
loop of CRY2, making it more CRY1-like and enhancing its affinity for CLOCK:BMAL1.
article_number: '55275'
article_processing_charge: No
article_type: original
author:
- first_name: Jennifer L
full_name: Fribourgh, Jennifer L
last_name: Fribourgh
- first_name: Ashutosh
full_name: Srivastava, Ashutosh
last_name: Srivastava
- first_name: Colby R
full_name: Sandate, Colby R
last_name: Sandate
- first_name: Alicia Kathleen
full_name: Michael, Alicia Kathleen
id: 6437c950-2a03-11ee-914d-d6476dd7b75c
last_name: Michael
- first_name: Peter L
full_name: Hsu, Peter L
last_name: Hsu
- first_name: Christin
full_name: Rakers, Christin
last_name: Rakers
- first_name: Leslee T
full_name: Nguyen, Leslee T
last_name: Nguyen
- first_name: Megan R
full_name: Torgrimson, Megan R
last_name: Torgrimson
- first_name: Gian Carlo G
full_name: Parico, Gian Carlo G
last_name: Parico
- first_name: Sarvind
full_name: Tripathi, Sarvind
last_name: Tripathi
- first_name: Ning
full_name: Zheng, Ning
last_name: Zheng
- first_name: Gabriel C
full_name: Lander, Gabriel C
last_name: Lander
- first_name: Tsuyoshi
full_name: Hirota, Tsuyoshi
last_name: Hirota
- first_name: Florence
full_name: Tama, Florence
last_name: Tama
- first_name: Carrie L
full_name: Partch, Carrie L
last_name: Partch
citation:
ama: Fribourgh JL, Srivastava A, Sandate CR, et al. Dynamics at the serine loop
underlie differential affinity of cryptochromes for CLOCK:BMAL1 to control circadian
timing. eLife. 2020;9. doi:10.7554/elife.55275
apa: Fribourgh, J. L., Srivastava, A., Sandate, C. R., Michael, A. K., Hsu, P. L.,
Rakers, C., … Partch, C. L. (2020). Dynamics at the serine loop underlie differential
affinity of cryptochromes for CLOCK:BMAL1 to control circadian timing. ELife.
eLife Sciences Publications. https://doi.org/10.7554/elife.55275
chicago: Fribourgh, Jennifer L, Ashutosh Srivastava, Colby R Sandate, Alicia K.
Michael, Peter L Hsu, Christin Rakers, Leslee T Nguyen, et al. “Dynamics at the
Serine Loop Underlie Differential Affinity of Cryptochromes for CLOCK:BMAL1 to
Control Circadian Timing.” ELife. eLife Sciences Publications, 2020. https://doi.org/10.7554/elife.55275.
ieee: J. L. Fribourgh et al., “Dynamics at the serine loop underlie differential
affinity of cryptochromes for CLOCK:BMAL1 to control circadian timing,” eLife,
vol. 9. eLife Sciences Publications, 2020.
ista: Fribourgh JL, Srivastava A, Sandate CR, Michael AK, Hsu PL, Rakers C, Nguyen
LT, Torgrimson MR, Parico GCG, Tripathi S, Zheng N, Lander GC, Hirota T, Tama
F, Partch CL. 2020. Dynamics at the serine loop underlie differential affinity
of cryptochromes for CLOCK:BMAL1 to control circadian timing. eLife. 9, 55275.
mla: Fribourgh, Jennifer L., et al. “Dynamics at the Serine Loop Underlie Differential
Affinity of Cryptochromes for CLOCK:BMAL1 to Control Circadian Timing.” ELife,
vol. 9, 55275, eLife Sciences Publications, 2020, doi:10.7554/elife.55275.
short: J.L. Fribourgh, A. Srivastava, C.R. Sandate, A.K. Michael, P.L. Hsu, C. Rakers,
L.T. Nguyen, M.R. Torgrimson, G.C.G. Parico, S. Tripathi, N. Zheng, G.C. Lander,
T. Hirota, F. Tama, C.L. Partch, ELife 9 (2020).
date_created: 2024-03-21T07:55:12Z
date_published: 2020-02-26T00:00:00Z
date_updated: 2024-03-25T12:25:02Z
day: '26'
doi: 10.7554/elife.55275
extern: '1'
intvolume: ' 9'
keyword:
- General Immunology and Microbiology
- General Biochemistry
- Genetics and Molecular Biology
- General Medicine
- General Neuroscience
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.7554/eLife.55275
month: '02'
oa: 1
oa_version: Published Version
publication: eLife
publication_identifier:
issn:
- 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: Dynamics at the serine loop underlie differential affinity of cryptochromes
for CLOCK:BMAL1 to control circadian timing
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 9
year: '2020'
...
---
_id: '15152'
abstract:
- lang: eng
text: Transcription factors (TFs) regulate gene expression through chromatin where
nucleosomes restrict DNA access. To study how TFs bind nucleosome-occupied motifs,
we focused on the reprogramming factors OCT4 and SOX2 in mouse embryonic stem
cells. We determined TF engagement throughout a nucleosome at base-pair resolution
in vitro, enabling structure determination by cryo–electron microscopy at two
preferred positions. Depending on motif location, OCT4 and SOX2 differentially
distort nucleosomal DNA. At one position, OCT4-SOX2 removes DNA from histone H2A
and histone H3; however, at an inverted motif, the TFs only induce local DNA distortions.
OCT4 uses one of its two DNA-binding domains to engage DNA in both structures,
reading out a partial motif. These findings explain site-specific nucleosome engagement
by the pluripotency factors OCT4 and SOX2, and they reveal how TFs distort nucleosomes
to access chromatinized motifs.
article_processing_charge: No
article_type: original
author:
- first_name: Alicia Kathleen
full_name: Michael, Alicia Kathleen
id: 6437c950-2a03-11ee-914d-d6476dd7b75c
last_name: Michael
orcid: 0000-0002-6080-839X
- first_name: Ralph S.
full_name: Grand, Ralph S.
last_name: Grand
- first_name: Luke
full_name: Isbel, Luke
last_name: Isbel
- first_name: Simone
full_name: Cavadini, Simone
last_name: Cavadini
- first_name: Zuzanna
full_name: Kozicka, Zuzanna
last_name: Kozicka
- first_name: Georg
full_name: Kempf, Georg
last_name: Kempf
- first_name: Richard D.
full_name: Bunker, Richard D.
last_name: Bunker
- first_name: Andreas D.
full_name: Schenk, Andreas D.
last_name: Schenk
- first_name: Alexandra
full_name: Graff-Meyer, Alexandra
last_name: Graff-Meyer
- first_name: Ganesh R.
full_name: Pathare, Ganesh R.
last_name: Pathare
- first_name: Joscha
full_name: Weiss, Joscha
last_name: Weiss
- first_name: Syota
full_name: Matsumoto, Syota
last_name: Matsumoto
- first_name: Lukas
full_name: Burger, Lukas
last_name: Burger
- first_name: Dirk
full_name: Schübeler, Dirk
last_name: Schübeler
- first_name: Nicolas H.
full_name: Thomä, Nicolas H.
last_name: Thomä
citation:
ama: Michael AK, Grand RS, Isbel L, et al. Mechanisms of OCT4-SOX2 motif readout
on nucleosomes. Science. 2020;368(6498):1460-1465. doi:10.1126/science.abb0074
apa: Michael, A. K., Grand, R. S., Isbel, L., Cavadini, S., Kozicka, Z., Kempf,
G., … Thomä, N. H. (2020). Mechanisms of OCT4-SOX2 motif readout on nucleosomes.
Science. American Association for the Advancement of Science . https://doi.org/10.1126/science.abb0074
chicago: Michael, Alicia K., Ralph S. Grand, Luke Isbel, Simone Cavadini, Zuzanna
Kozicka, Georg Kempf, Richard D. Bunker, et al. “Mechanisms of OCT4-SOX2 Motif
Readout on Nucleosomes.” Science. American Association for the Advancement
of Science , 2020. https://doi.org/10.1126/science.abb0074.
ieee: A. K. Michael et al., “Mechanisms of OCT4-SOX2 motif readout on nucleosomes,”
Science, vol. 368, no. 6498. American Association for the Advancement of
Science , pp. 1460–1465, 2020.
ista: Michael AK, Grand RS, Isbel L, Cavadini S, Kozicka Z, Kempf G, Bunker RD,
Schenk AD, Graff-Meyer A, Pathare GR, Weiss J, Matsumoto S, Burger L, Schübeler
D, Thomä NH. 2020. Mechanisms of OCT4-SOX2 motif readout on nucleosomes. Science.
368(6498), 1460–1465.
mla: Michael, Alicia K., et al. “Mechanisms of OCT4-SOX2 Motif Readout on Nucleosomes.”
Science, vol. 368, no. 6498, American Association for the Advancement of
Science , 2020, pp. 1460–65, doi:10.1126/science.abb0074.
short: A.K. Michael, R.S. Grand, L. Isbel, S. Cavadini, Z. Kozicka, G. Kempf, R.D.
Bunker, A.D. Schenk, A. Graff-Meyer, G.R. Pathare, J. Weiss, S. Matsumoto, L.
Burger, D. Schübeler, N.H. Thomä, Science 368 (2020) 1460–1465.
date_created: 2024-03-21T07:54:44Z
date_published: 2020-04-23T00:00:00Z
date_updated: 2024-03-25T12:29:34Z
day: '23'
doi: 10.1126/science.abb0074
extern: '1'
intvolume: ' 368'
issue: '6498'
language:
- iso: eng
month: '04'
oa_version: None
page: 1460-1465
publication: Science
publication_identifier:
eissn:
- 1095-9203
issn:
- 0036-8075
publication_status: published
publisher: 'American Association for the Advancement of Science '
quality_controlled: '1'
scopus_import: '1'
status: public
title: Mechanisms of OCT4-SOX2 motif readout on nucleosomes
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 368
year: '2020'
...
---
_id: '7525'
abstract:
- lang: eng
text: "The medial habenula (MHb) is an evolutionary conserved epithalamic structure
important for the modulation of emotional memory. It is involved in regulation
of anxiety, compulsive behavior, addiction (nicotinic and opioid), sexual and
feeding behavior. MHb receives inputs from septal regions and projects exclusively
to the interpeduncular nucleus (IPN). Distinct sub-regions of the septum project
to different subnuclei of MHb: the bed nucleus of anterior commissure projects
to dorsal MHb and the triangular septum projects to ventral MHb. Furthermore,
the dorsal and ventral MHb project to the lateral and rostral/central IPN, respectively.
Importantly, these projections have unique features of prominent co-release of
different neurotransmitters and requirement of a peculiar type of calcium channel
for release. In general, synaptic neurotransmission requires an activity-dependent
influx of Ca2+ into the presynaptic terminal through voltage-gated calcium channels.
The calcium channel family most commonly involved in neurotransmitter release
comprises three members, P/Q-, N- and R-type with Cav2.1, Cav2.2 and Cav2.3 subunits,
respectively. In contrast to most CNS synapses that mainly express Cav2.1 and/or
Cav2.2, MHb terminals in the IPN exclusively express Cav2.3. In other parts of
the brain, such as the hippocampus, Cav2.3 is mostly located to postsynaptic elements.
This unusual presynaptic location of Cav2.3 in the MHb-IPN pathway implies unique
mechanisms of glutamate release in this pathway. One potential example of such
uniqueness is the facilitation of release by GABAB receptor (GBR) activation.
Presynaptic GBRs usually inhibit the release of neurotransmitters by inhibiting
presynaptic calcium channels. MHb shows the highest expression levels of GBR in
the brain. GBRs comprise two subunits, GABAB1 (GB1) and GABAB2 (GB2), and are
associated with auxiliary subunits, called potassium channel tetramerization domain
containing proteins (KCTD) 8, 12, 12b and 16. Among these four subunits, KCTD12b
is exclusively expressed in ventral MHb, and KCTD8 shows the strongest expression
in the whole MHb among other brain regions, indicating that KCTD8 and KCTD12b
may be involved in the unique mechanisms of neurotransmitter release mediated
by Cav2.3 and regulated by GBRs in this pathway. \r\nIn the present study, we
first verified that neurotransmission in both dorsal and ventral MHb-IPN pathways
is mainly mediated by Cav2.3 using a selective blocker of R-type channels, SNX-482.
We next found that baclofen, a GBR agonist, has facilitatory effects on release
from ventral MHb terminal in rostral IPN, whereas it has inhibitory effects on
release from dorsal MHb terminals in lateral IPN, indicating that KCTD12b expressed
exclusively in ventral MHb may have a role in the facilitatory effects of GBR
activation. In a heterologous expression system using HEK cells, we found that
KCTD8 and KCTD12b but not KCTD12 directly bind with Cav2.3. Pre-embedding immunogold
electron microscopy data show that Cav2.3 and KCTD12b are distributed most densely
in presynaptic active zone in IPN with KCTD12b being present only in rostral/central
but not lateral IPN, whereas GABAB, KCTD8 and KCTD12 are distributed most densely
in perisynaptic sites with KCTD12 present more frequently in postsynaptic elements
and only in rostral/central IPN. In freeze-fracture replica labelling, Cav2.3,
KCTD8 and KCTD12b are co-localized with each other in the same active zone indicating
that they may form complexes regulating vesicle release in rostral IPN. \r\nOn
electrophysiological studies of wild type (WT) mice, we found that paired-pulse
ratio in rostral IPN of KCTD12b knock-out (KO) mice is lower than those of WT
and KCTD8 KO mice. Consistent with this finding, in mean variance analysis, release
probability in rostral IPN of KCTD12b KO mice is higher than that of WT and KCTD8
KO mice. Although paired-pulse ratios are not different between WT and KCTD8 KO
mice, the mean variance analysis revealed significantly lower release probability
in rostral IPN of KCTD8 KO than WT mice. These results demonstrate bidirectional
regulation of Cav2.3-mediated release by KCTD8 and KCTD12b without GBR activation
in rostral IPN. Finally, we examined the baclofen effects in rostral IPN of KCTD8
and KCTD12b KO mice, and found the facilitation of release remained in both KO
mice, indicating that the peculiar effects of the GBR activation in this pathway
do not depend on the selective expression of these KCTD subunits in ventral MHb.
However, we found that presynaptic potentiation of evoked EPSC amplitude by baclofen
falls to baseline after washout faster in KCTD12b KO mice than WT, KCTD8 KO and
KCTD8/12b double KO mice. This result indicates that KCTD12b is involved in sustained
potentiation of vesicle release by GBR activation, whereas KCTD8 is involved in
its termination in the absence of KCTD12b. Consistent with these functional findings,
replica labelling revealed an increase in density of KCTD8, but not Cav2.3 or
GBR at active zone in rostral IPN of KCTD12b KO mice compared with that of WT
mice, suggesting that increased association of KCTD8 with Cav2.3 facilitates the
release probability and termination of the GBR effect in the absence of KCTD12b.\r\nIn
summary, our study provided new insights into the physiological roles of presynaptic
Cav2.3, GBRs and their auxiliary subunits KCTDs at an evolutionary conserved neuronal
circuit. Future studies will be required to identify the exact molecular mechanism
underlying the GBR-mediated presynaptic potentiation on ventral MHb terminals.
It remains to be determined whether the prominent presence of presynaptic KCTDs
at active zone could exert similar neuromodulatory functions in different pathways
of the brain.\r\n"
acknowledged_ssus:
- _id: EM-Fac
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Pradeep
full_name: Bhandari, Pradeep
id: 45EDD1BC-F248-11E8-B48F-1D18A9856A87
last_name: Bhandari
orcid: 0000-0003-0863-4481
citation:
ama: Bhandari P. Localization and functional role of Cav2.3 in the medial habenula
to interpeduncular nucleus pathway. 2020. doi:10.15479/AT:ISTA:7525
apa: Bhandari, P. (2020). Localization and functional role of Cav2.3 in the medial
habenula to interpeduncular nucleus pathway. Institute of Science and Technology
Austria. https://doi.org/10.15479/AT:ISTA:7525
chicago: Bhandari, Pradeep. “Localization and Functional Role of Cav2.3 in the Medial
Habenula to Interpeduncular Nucleus Pathway.” Institute of Science and Technology
Austria, 2020. https://doi.org/10.15479/AT:ISTA:7525.
ieee: P. Bhandari, “Localization and functional role of Cav2.3 in the medial habenula
to interpeduncular nucleus pathway,” Institute of Science and Technology Austria,
2020.
ista: Bhandari P. 2020. Localization and functional role of Cav2.3 in the medial
habenula to interpeduncular nucleus pathway. Institute of Science and Technology
Austria.
mla: Bhandari, Pradeep. Localization and Functional Role of Cav2.3 in the Medial
Habenula to Interpeduncular Nucleus Pathway. Institute of Science and Technology
Austria, 2020, doi:10.15479/AT:ISTA:7525.
short: P. Bhandari, Localization and Functional Role of Cav2.3 in the Medial Habenula
to Interpeduncular Nucleus Pathway, Institute of Science and Technology Austria,
2020.
date_created: 2020-02-26T10:56:37Z
date_published: 2020-02-28T00:00:00Z
date_updated: 2023-09-07T13:20:03Z
day: '28'
ddc:
- '570'
degree_awarded: PhD
department:
- _id: RySh
doi: 10.15479/AT:ISTA:7525
file:
- access_level: open_access
checksum: 4589234fdb12b4ad72273b311723a7b4
content_type: application/pdf
creator: pbhandari
date_created: 2020-02-28T08:37:53Z
date_updated: 2021-03-01T23:30:04Z
embargo: 2021-02-28
file_id: '7538'
file_name: Pradeep Bhandari Thesis.pdf
file_size: 9646346
relation: main_file
title: Localization and functional role of Cav2.3 in the medial habenula to interpeduncular
nucleus pathway
- access_level: closed
checksum: aa79490553ca0a5c9b6fbcd152e93928
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: pbhandari
date_created: 2020-02-28T08:47:14Z
date_updated: 2021-03-01T23:30:04Z
embargo_to: open_access
file_id: '7539'
file_name: Pradeep Bhandari Thesis.docx
file_size: 35252164
relation: source_file
title: Localization and functional role of Cav2.3 in the medial habenula to interpeduncular
nucleus pathway
file_date_updated: 2021-03-01T23:30:04Z
has_accepted_license: '1'
keyword:
- Cav2.3
- medial habenula (MHb)
- interpeduncular nucleus (IPN)
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: '79'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
status: public
supervisor:
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
title: Localization and functional role of Cav2.3 in the medial habenula to interpeduncular
nucleus pathway
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2020'
...
---
_id: '8586'
abstract:
- lang: eng
text: Cryo-electron microscopy (cryo-EM) of cellular specimens provides insights
into biological processes and structures within a native context. However, a major
challenge still lies in the efficient and reproducible preparation of adherent
cells for subsequent cryo-EM analysis. This is due to the sensitivity of many
cellular specimens to the varying seeding and culturing conditions required for
EM experiments, the often limited amount of cellular material and also the fragility
of EM grids and their substrate. Here, we present low-cost and reusable 3D printed
grid holders, designed to improve specimen preparation when culturing challenging
cellular samples directly on grids. The described grid holders increase cell culture
reproducibility and throughput, and reduce the resources required for cell culturing.
We show that grid holders can be integrated into various cryo-EM workflows, including
micro-patterning approaches to control cell seeding on grids, and for generating
samples for cryo-focused ion beam milling and cryo-electron tomography experiments.
Their adaptable design allows for the generation of specialized grid holders customized
to a large variety of applications.
acknowledged_ssus:
- _id: ScienComp
- _id: LifeSc
- _id: Bio
- _id: EM-Fac
acknowledgement: This work was supported by the Austrian Science Fund (FWF, P33367)
to FKMS. BZ acknowledges support by the Niederösterreich Fond. This research was
also supported by the Scientific Service Units (SSU) of IST Austria through resources
provided by Scientific Computing (SciComp), the Life Science Facility (LSF), the
BioImaging Facility (BIF) and the Electron Microscopy Facility (EMF). We thank Georgi
Dimchev (IST Austria) and Sonja Jacob (Vienna Biocenter Core Facilities) for testing
our grid holders in different experimental setups and Daniel Gütl and the Kondrashov
group (IST Austria) for granting us repeated access to their 3D printers. We also
thank Jonna Alanko and the Sixt lab (IST Austria) for providing us HeLa cells, primary
BL6 mouse tail fibroblasts, NIH 3T3 fibroblasts and human telomerase immortalised
foreskin fibroblasts for our experiments. We are thankful to Ori Avinoam and William
Wan for helpful comments on the manuscript and also thank Dorotea Fracchiolla (Art&Science)
for illustrating the graphical abstract.
article_number: '107633'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Florian
full_name: Fäßler, Florian
id: 404F5528-F248-11E8-B48F-1D18A9856A87
last_name: Fäßler
orcid: 0000-0001-7149-769X
- first_name: Bettina
full_name: Zens, Bettina
id: 45FD126C-F248-11E8-B48F-1D18A9856A87
last_name: Zens
orcid: 0000-0002-9561-1239
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Florian KM
full_name: Schur, Florian KM
id: 48AD8942-F248-11E8-B48F-1D18A9856A87
last_name: Schur
orcid: 0000-0003-4790-8078
citation:
ama: Fäßler F, Zens B, Hauschild R, Schur FK. 3D printed cell culture grid holders
for improved cellular specimen preparation in cryo-electron microscopy. Journal
of Structural Biology. 2020;212(3). doi:10.1016/j.jsb.2020.107633
apa: Fäßler, F., Zens, B., Hauschild, R., & Schur, F. K. (2020). 3D printed
cell culture grid holders for improved cellular specimen preparation in cryo-electron
microscopy. Journal of Structural Biology. Elsevier. https://doi.org/10.1016/j.jsb.2020.107633
chicago: Fäßler, Florian, Bettina Zens, Robert Hauschild, and Florian KM Schur.
“3D Printed Cell Culture Grid Holders for Improved Cellular Specimen Preparation
in Cryo-Electron Microscopy.” Journal of Structural Biology. Elsevier,
2020. https://doi.org/10.1016/j.jsb.2020.107633.
ieee: F. Fäßler, B. Zens, R. Hauschild, and F. K. Schur, “3D printed cell culture
grid holders for improved cellular specimen preparation in cryo-electron microscopy,”
Journal of Structural Biology, vol. 212, no. 3. Elsevier, 2020.
ista: Fäßler F, Zens B, Hauschild R, Schur FK. 2020. 3D printed cell culture grid
holders for improved cellular specimen preparation in cryo-electron microscopy.
Journal of Structural Biology. 212(3), 107633.
mla: Fäßler, Florian, et al. “3D Printed Cell Culture Grid Holders for Improved
Cellular Specimen Preparation in Cryo-Electron Microscopy.” Journal of Structural
Biology, vol. 212, no. 3, 107633, Elsevier, 2020, doi:10.1016/j.jsb.2020.107633.
short: F. Fäßler, B. Zens, R. Hauschild, F.K. Schur, Journal of Structural Biology
212 (2020).
date_created: 2020-09-29T13:24:06Z
date_published: 2020-12-01T00:00:00Z
date_updated: 2024-03-27T23:30:05Z
day: '01'
ddc:
- '570'
department:
- _id: FlSc
doi: 10.1016/j.jsb.2020.107633
external_id:
isi:
- '000600997800008'
file:
- access_level: open_access
checksum: c48cbf594e84fc2f91966ffaafc0918c
content_type: application/pdf
creator: dernst
date_created: 2020-12-10T14:01:10Z
date_updated: 2020-12-10T14:01:10Z
file_id: '8937'
file_name: 2020_JourStrucBiology_Faessler.pdf
file_size: 7076870
relation: main_file
success: 1
file_date_updated: 2020-12-10T14:01:10Z
has_accepted_license: '1'
intvolume: ' 212'
isi: 1
issue: '3'
keyword:
- electron microscopy
- cryo-EM
- EM sample preparation
- 3D printing
- cell culture
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
project:
- _id: 9B954C5C-BA93-11EA-9121-9846C619BF3A
grant_number: P33367
name: Structure and isoform diversity of the Arp2/3 complex
- _id: 059B463C-7A3F-11EA-A408-12923DDC885E
name: NÖ-Fonds Preis für die Jungforscherin des Jahres am IST Austria
publication: Journal of Structural Biology
publication_identifier:
issn:
- 1047-8477
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
record:
- id: '14592'
relation: used_in_publication
status: public
- id: '12491'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: 3D printed cell culture grid holders for improved cellular specimen preparation
in cryo-electron microscopy
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 212
year: '2020'
...
---
_id: '8657'
abstract:
- lang: eng
text: "Synthesis of proteins – translation – is a fundamental process of life. Quantitative
studies anchor translation into the context of bacterial physiology and reveal
several mathematical relationships, called “growth laws,” which capture physiological
feedbacks between protein synthesis and cell growth. Growth laws describe the
dependency of the ribosome abundance as a function of growth rate, which can change
depending on the growth conditions. Perturbations of translation reveal that bacteria
employ a compensatory strategy in which the reduced translation capability results
in increased expression of the translation machinery.\r\nPerturbations of translation
are achieved in various ways; clinically interesting is the application of translation-targeting
antibiotics – translation inhibitors. The antibiotic effects on bacterial physiology
are often poorly understood. Bacterial responses to two or more simultaneously
applied antibiotics are even more puzzling. The combined antibiotic effect determines
the type of drug interaction, which ranges from synergy (the effect is stronger
than expected) to antagonism (the effect is weaker) and suppression (one of the
drugs loses its potency).\r\nIn the first part of this work, we systematically
measure the pairwise interaction network for translation inhibitors that interfere
with different steps in translation. We find that the interactions are surprisingly
diverse and tend to be more antagonistic. To explore the underlying mechanisms,
we begin with a minimal biophysical model of combined antibiotic action. We base
this model on the kinetics of antibiotic uptake and binding together with the
physiological response described by the growth laws. The biophysical model explains
some drug interactions, but not all; it specifically fails to predict suppression.\r\nIn
the second part of this work, we hypothesize that elusive suppressive drug interactions
result from the interplay between ribosomes halted in different stages of translation.
To elucidate this putative mechanism of drug interactions between translation
inhibitors, we generate translation bottlenecks genetically using in- ducible
control of translation factors that regulate well-defined translation cycle steps.
These perturbations accurately mimic antibiotic action and drug interactions,
supporting that the interplay of different translation bottlenecks partially causes
these interactions.\r\nWe extend this approach by varying two translation bottlenecks
simultaneously. This approach reveals the suppression of translocation inhibition
by inhibited translation. We rationalize this effect by modeling dense traffic
of ribosomes that move on transcripts in a translation factor-mediated manner.
This model predicts a dissolution of traffic jams caused by inhibited translocation
when the density of ribosome traffic is reduced by lowered initiation. We base
this model on the growth laws and quantitative relationships between different
translation and growth parameters.\r\nIn the final part of this work, we describe
a set of tools aimed at quantification of physiological and translation parameters.
We further develop a simple model that directly connects the abundance of a translation
factor with the growth rate, which allows us to extract physiological parameters
describing initiation. We demonstrate the development of tools for measuring translation
rate.\r\nThis thesis showcases how a combination of high-throughput growth rate
mea- surements, genetics, and modeling can reveal mechanisms of drug interactions.
Furthermore, by a gradual transition from combinations of antibiotics to precise
genetic interventions, we demonstrated the equivalency between genetic and chemi-
cal perturbations of translation. These findings tile the path for quantitative
studies of antibiotic combinations and illustrate future approaches towards the
quantitative description of translation."
acknowledged_ssus:
- _id: LifeSc
- _id: M-Shop
acknowledgement: I thank Life Science Facilities for their continuous support with
providing top-notch laboratory materials, keeping the devices humming, and coordinating
the repairs and building of custom-designed laboratory equipment with the MIBA Machine
shop.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Bor
full_name: Kavcic, Bor
id: 350F91D2-F248-11E8-B48F-1D18A9856A87
last_name: Kavcic
orcid: 0000-0001-6041-254X
citation:
ama: 'Kavcic B. Perturbations of protein synthesis: from antibiotics to genetics
and physiology. 2020. doi:10.15479/AT:ISTA:8657'
apa: 'Kavcic, B. (2020). Perturbations of protein synthesis: from antibiotics
to genetics and physiology. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:8657'
chicago: 'Kavcic, Bor. “Perturbations of Protein Synthesis: From Antibiotics to
Genetics and Physiology.” Institute of Science and Technology Austria, 2020. https://doi.org/10.15479/AT:ISTA:8657.'
ieee: 'B. Kavcic, “Perturbations of protein synthesis: from antibiotics to genetics
and physiology,” Institute of Science and Technology Austria, 2020.'
ista: 'Kavcic B. 2020. Perturbations of protein synthesis: from antibiotics to genetics
and physiology. Institute of Science and Technology Austria.'
mla: 'Kavcic, Bor. Perturbations of Protein Synthesis: From Antibiotics to Genetics
and Physiology. Institute of Science and Technology Austria, 2020, doi:10.15479/AT:ISTA:8657.'
short: 'B. Kavcic, Perturbations of Protein Synthesis: From Antibiotics to Genetics
and Physiology, Institute of Science and Technology Austria, 2020.'
date_created: 2020-10-13T16:46:14Z
date_published: 2020-10-14T00:00:00Z
date_updated: 2023-09-07T13:20:48Z
day: '14'
ddc:
- '571'
- '530'
- '570'
degree_awarded: PhD
department:
- _id: GaTk
doi: 10.15479/AT:ISTA:8657
file:
- access_level: open_access
checksum: d708ecd62b6fcc3bc1feb483b8dbe9eb
content_type: application/pdf
creator: bkavcic
date_created: 2020-10-15T06:41:20Z
date_updated: 2021-10-07T22:30:03Z
embargo: 2021-10-06
file_id: '8663'
file_name: kavcicB_thesis202009.pdf
file_size: 52636162
relation: main_file
- access_level: closed
checksum: bb35f2352a04db19164da609f00501f3
content_type: application/zip
creator: bkavcic
date_created: 2020-10-15T06:41:53Z
date_updated: 2021-10-07T22:30:03Z
embargo_to: open_access
file_id: '8664'
file_name: 2020b.zip
file_size: 321681247
relation: source_file
file_date_updated: 2021-10-07T22:30:03Z
has_accepted_license: '1'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
page: '271'
publication_identifier:
isbn:
- 978-3-99078-011-4
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '7673'
relation: part_of_dissertation
status: public
- id: '8250'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Gašper
full_name: Tkačik, Gašper
id: 3D494DCA-F248-11E8-B48F-1D18A9856A87
last_name: Tkačik
orcid: 0000-0002-6699-1455
- first_name: Mark Tobias
full_name: Bollenbach, Mark Tobias
id: 3E6DB97A-F248-11E8-B48F-1D18A9856A87
last_name: Bollenbach
orcid: 0000-0003-4398-476X
title: 'Perturbations of protein synthesis: from antibiotics to genetics and physiology'
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2020'
...