---
_id: '3092'
abstract:
- lang: eng
text: The phytohormone auxin is vital to plant growth and development. A unique
property of auxin among all other plant hormones is its cell-to-cell polar transport
that requires activity of polarly localized PIN-FORMED (PIN) auxin efflux transporters.
Despite the substantial molecular insight into the cellular PIN polarization,
the mechanistic understanding for developmentally and environmentally regulated
PIN polarization is scarce. The long-standing belief that auxin modulates its
own transport by means of a positive feedback mechanism has inspired both experimentalists
and theoreticians for more than two decades. Recently, theoretical models for
auxin-dependent patterning in plants include the feedback between auxin transport
and the PIN protein localization. These computer models aid to assess the complexity
of plant development by testing and predicting plausible scenarios for various
developmental processes that occur in planta. Although the majority of these models
rely on purely heuristic principles, the most recent mechanistic models tentatively
integrate biologically testable components into known cellular processes that
underlie the PIN polarity regulation. The existing and emerging computational
approaches to describe PIN polarization are presented and discussed in the light
of recent experimental data on the PIN polar targeting.
author:
- first_name: Krzysztof T
full_name: Wabnik, Krzysztof T
id: 4DE369A4-F248-11E8-B48F-1D18A9856A87
last_name: Wabnik
orcid: 0000-0001-7263-0560
- first_name: Willy
full_name: Govaerts, Willy
last_name: Govaerts
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Jürgen
full_name: Kleine Vehn, Jürgen
last_name: Kleine Vehn
citation:
ama: 'Wabnik KT, Govaerts W, Friml J, Kleine Vehn J. Feedback models for polarized
auxin transport: An emerging trend. Molecular BioSystems. 2011;7(8):2352-2359.
doi:10.1039/c1mb05109a'
apa: 'Wabnik, K. T., Govaerts, W., Friml, J., & Kleine Vehn, J. (2011). Feedback
models for polarized auxin transport: An emerging trend. Molecular BioSystems.
Royal Society of Chemistry. https://doi.org/10.1039/c1mb05109a'
chicago: 'Wabnik, Krzysztof T, Willy Govaerts, Jiří Friml, and Jürgen Kleine Vehn.
“Feedback Models for Polarized Auxin Transport: An Emerging Trend.” Molecular
BioSystems. Royal Society of Chemistry, 2011. https://doi.org/10.1039/c1mb05109a.'
ieee: 'K. T. Wabnik, W. Govaerts, J. Friml, and J. Kleine Vehn, “Feedback models
for polarized auxin transport: An emerging trend,” Molecular BioSystems,
vol. 7, no. 8. Royal Society of Chemistry, pp. 2352–2359, 2011.'
ista: 'Wabnik KT, Govaerts W, Friml J, Kleine Vehn J. 2011. Feedback models for
polarized auxin transport: An emerging trend. Molecular BioSystems. 7(8), 2352–2359.'
mla: 'Wabnik, Krzysztof T., et al. “Feedback Models for Polarized Auxin Transport:
An Emerging Trend.” Molecular BioSystems, vol. 7, no. 8, Royal Society
of Chemistry, 2011, pp. 2352–59, doi:10.1039/c1mb05109a.'
short: K.T. Wabnik, W. Govaerts, J. Friml, J. Kleine Vehn, Molecular BioSystems
7 (2011) 2352–2359.
date_created: 2018-12-11T12:01:20Z
date_published: 2011-06-10T00:00:00Z
date_updated: 2021-01-12T07:41:00Z
day: '10'
doi: 10.1039/c1mb05109a
extern: '1'
external_id:
pmid:
- '21660355'
intvolume: ' 7'
issue: '8'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pubmed/21660355
month: '06'
oa: 1
oa_version: Published Version
page: 2352 - 2359
pmid: 1
publication: Molecular BioSystems
publication_status: published
publisher: Royal Society of Chemistry
publist_id: '3608'
quality_controlled: '1'
status: public
title: 'Feedback models for polarized auxin transport: An emerging trend'
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 7
year: '2011'
...
---
_id: '3089'
abstract:
- lang: eng
text: The phytohormone auxin is an important determinant of plant development. Directional
auxin flow within tissues depends on polar localization of PIN auxin transporters.
To explore regulation of PIN-mediated auxin transport, we screened for suppressors
of PIN1 overexpression (supo) and identified an inositol polyphosphate 1-phosphatase
mutant (supo1), with elevated inositol trisphosphate (InsP 3) and cytosolic Ca
2+ levels. Pharmacological and genetic increases in InsP 3 or Ca 2+ levels also
suppressed the PIN1 gain-of-function phenotypes and caused defects in basal PIN
localization, auxin transport and auxin-mediated development. In contrast, the
reductions in InsP 3 levels and Ca 2+ signaling antagonized the effects of the
supo1 mutation and disrupted preferentially apical PIN localization. InsP 3 and
Ca 2+ are evolutionarily conserved second messengers involved in various cellular
functions, particularly stress responses. Our findings implicate them as modifiers
of cell polarity and polar auxin transport, and highlight a potential integration
point through which Ca 2+ signaling-related stimuli could influence auxin-mediated
development.
author:
- first_name: Jing
full_name: Zhang, Jing
last_name: Zhang
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Philip
full_name: Brewer, Philip B
last_name: Brewer
- first_name: Marta
full_name: Michniewicz, Marta
last_name: Michniewicz
- first_name: Peter
full_name: Peter Grones
id: 399876EC-F248-11E8-B48F-1D18A9856A87
last_name: Grones
- first_name: Jürgen
full_name: Kleine-Vehn, Jürgen
last_name: Kleine Vehn
- first_name: Christian
full_name: Löfke, Christian
last_name: Löfke
- first_name: Thomas
full_name: Teichmann, Thomas
last_name: Teichmann
- first_name: Agnieszka
full_name: Bielach, Agnieszka
last_name: Bielach
- first_name: Bernard
full_name: Cannoot, Bernard
last_name: Cannoot
- first_name: Klára
full_name: Hoyerová, Klára
last_name: Hoyerová
- first_name: Xu
full_name: Xu Chen
id: 4E5ADCAA-F248-11E8-B48F-1D18A9856A87
last_name: Chen
- first_name: Hong
full_name: Xue, Hong-Wei
last_name: Xue
- first_name: Eva
full_name: Eva Benková
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Eva
full_name: Zažímalová, Eva
last_name: Zažímalová
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Zhang J, Vanneste S, Brewer P, et al. Inositol trisphosphate-induced ca^2+
signaling modulates auxin transport and pin polarity. Developmental Cell.
2011;20(6):855-866. doi:10.1016/j.devcel.2011.05.013
apa: Zhang, J., Vanneste, S., Brewer, P., Michniewicz, M., Grones, P., Kleine Vehn,
J., … Friml, J. (2011). Inositol trisphosphate-induced ca^2+ signaling modulates
auxin transport and pin polarity. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2011.05.013
chicago: Zhang, Jing, Steffen Vanneste, Philip Brewer, Marta Michniewicz, Peter
Grones, Jürgen Kleine Vehn, Christian Löfke, et al. “Inositol Trisphosphate-Induced
Ca^2+ Signaling Modulates Auxin Transport and Pin Polarity.” Developmental
Cell. Cell Press, 2011. https://doi.org/10.1016/j.devcel.2011.05.013.
ieee: J. Zhang et al., “Inositol trisphosphate-induced ca^2+ signaling modulates
auxin transport and pin polarity,” Developmental Cell, vol. 20, no. 6.
Cell Press, pp. 855–866, 2011.
ista: Zhang J, Vanneste S, Brewer P, Michniewicz M, Grones P, Kleine Vehn J, Löfke
C, Teichmann T, Bielach A, Cannoot B, Hoyerová K, Chen X, Xue H, Benková E, Zažímalová
E, Friml J. 2011. Inositol trisphosphate-induced ca^2+ signaling modulates auxin
transport and pin polarity. Developmental Cell. 20(6), 855–866.
mla: Zhang, Jing, et al. “Inositol Trisphosphate-Induced Ca^2+ Signaling Modulates
Auxin Transport and Pin Polarity.” Developmental Cell, vol. 20, no. 6,
Cell Press, 2011, pp. 855–66, doi:10.1016/j.devcel.2011.05.013.
short: J. Zhang, S. Vanneste, P. Brewer, M. Michniewicz, P. Grones, J. Kleine Vehn,
C. Löfke, T. Teichmann, A. Bielach, B. Cannoot, K. Hoyerová, X. Chen, H. Xue,
E. Benková, E. Zažímalová, J. Friml, Developmental Cell 20 (2011) 855–866.
date_created: 2018-12-11T12:01:18Z
date_published: 2011-06-14T00:00:00Z
date_updated: 2021-01-12T07:40:58Z
day: '14'
doi: 10.1016/j.devcel.2011.05.013
extern: 1
intvolume: ' 20'
issue: '6'
month: '06'
page: 855 - 866
publication: Developmental Cell
publication_status: published
publisher: Cell Press
publist_id: '3612'
quality_controlled: 0
status: public
title: Inositol trisphosphate-induced ca^2+ signaling modulates auxin transport and
pin polarity
type: journal_article
volume: 20
year: '2011'
...
---
_id: '3090'
abstract:
- lang: eng
text: The polarized transport of the phytohormone auxin [1], which is crucial for
the regulation of different stages of plant development [2, 3], depends on the
asymmetric plasma membrane distribution of the PIN-FORMED (PIN) auxin efflux carriers
[4, 5]. The PIN polar localization results from clathrin-mediated endocytosis
(CME) from the plasma membrane and subsequent polar recycling [6]. The Arabidopsis
genome encodes two groups of dynamin-related proteins (DRPs) that show homology
to mammalian dynamin - a protein required for fission of endocytic vesicles during
CME [7, 8]. Here we show by coimmunoprecipitation (coIP), bimolecular fluorescence
complementation (BiFC), and Förster resonance energy transfer (FRET) that members
of the DRP1 group closely associate with PIN proteins at the cell plate. Localization
and phenotypic analysis of novel drp1 mutants revealed a requirement for DRP1
function in correct PIN distribution and in auxin-mediated development. We propose
that rapid and specific internalization of PIN proteins mediated by the DRP1 proteins
and the associated CME machinery from the cell plate membranes during cytokinesis
is an important mechanism for proper polar PIN positioning in interphase cells.
author:
- first_name: Jozef
full_name: Mravec, Jozef
last_name: Mravec
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Na
full_name: Li, Na
last_name: Li
- first_name: Sjef
full_name: Boeren, Sjef
last_name: Boeren
- first_name: Rumyana
full_name: Karlova, Rumyana
last_name: Karlova
- first_name: Saeko
full_name: Kitakura, Saeko
last_name: Kitakura
- first_name: Markéta
full_name: Pařezová, Markéta
last_name: Pařezová
- first_name: Satoshi
full_name: Naramoto, Satoshi
last_name: Naramoto
- first_name: Thomasz
full_name: Nodzyński, Thomasz
last_name: Nodzyński
- first_name: Pankaj
full_name: Dhonukshe, Pankaj
last_name: Dhonukshe
- first_name: Sebastian
full_name: Bednarek, Sebastian Y
last_name: Bednarek
- first_name: Eva
full_name: Zažímalová, Eva
last_name: Zažímalová
- first_name: Sacco
full_name: De Vries, Sacco
last_name: De Vries
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Mravec J, Petrášek J, Li N, et al. Cell plate restricted association of DRP1A
and PIN proteins is required for cell polarity establishment in arabidopsis. Current
Biology. 2011;21(12):1055-1060. doi:10.1016/j.cub.2011.05.018
apa: Mravec, J., Petrášek, J., Li, N., Boeren, S., Karlova, R., Kitakura, S., …
Friml, J. (2011). Cell plate restricted association of DRP1A and PIN proteins
is required for cell polarity establishment in arabidopsis. Current Biology.
Cell Press. https://doi.org/10.1016/j.cub.2011.05.018
chicago: Mravec, Jozef, Jan Petrášek, Na Li, Sjef Boeren, Rumyana Karlova, Saeko
Kitakura, Markéta Pařezová, et al. “Cell Plate Restricted Association of DRP1A
and PIN Proteins Is Required for Cell Polarity Establishment in Arabidopsis.”
Current Biology. Cell Press, 2011. https://doi.org/10.1016/j.cub.2011.05.018.
ieee: J. Mravec et al., “Cell plate restricted association of DRP1A and PIN
proteins is required for cell polarity establishment in arabidopsis,” Current
Biology, vol. 21, no. 12. Cell Press, pp. 1055–1060, 2011.
ista: Mravec J, Petrášek J, Li N, Boeren S, Karlova R, Kitakura S, Pařezová M, Naramoto
S, Nodzyński T, Dhonukshe P, Bednarek S, Zažímalová E, De Vries S, Friml J. 2011.
Cell plate restricted association of DRP1A and PIN proteins is required for cell
polarity establishment in arabidopsis. Current Biology. 21(12), 1055–1060.
mla: Mravec, Jozef, et al. “Cell Plate Restricted Association of DRP1A and PIN Proteins
Is Required for Cell Polarity Establishment in Arabidopsis.” Current Biology,
vol. 21, no. 12, Cell Press, 2011, pp. 1055–60, doi:10.1016/j.cub.2011.05.018.
short: J. Mravec, J. Petrášek, N. Li, S. Boeren, R. Karlova, S. Kitakura, M. Pařezová,
S. Naramoto, T. Nodzyński, P. Dhonukshe, S. Bednarek, E. Zažímalová, S. De Vries,
J. Friml, Current Biology 21 (2011) 1055–1060.
date_created: 2018-12-11T12:01:19Z
date_published: 2011-06-21T00:00:00Z
date_updated: 2021-01-12T07:40:59Z
day: '21'
doi: 10.1016/j.cub.2011.05.018
extern: 1
intvolume: ' 21'
issue: '12'
month: '06'
page: 1055 - 1060
publication: Current Biology
publication_status: published
publisher: Cell Press
publist_id: '3611'
quality_controlled: 0
status: public
title: Cell plate restricted association of DRP1A and PIN proteins is required for
cell polarity establishment in arabidopsis
type: journal_article
volume: 21
year: '2011'
...
---
_id: '3088'
abstract:
- lang: eng
text: 'Background: Whereas the majority of animals develop toward a predetermined
body plan, plants show iterative growth and continually produce new organs and
structures from actively dividing meristems. This raises an intriguing question:
How are these newly developed organs patterned? In Arabidopsis embryos, radial
symmetry is broken by the bisymmetric specification of the cotyledons in the apical
domain. Subsequently, this bisymmetry is propagated to the root promeristem. Results:
Here we present a mutually inhibitory feedback loop between auxin and cytokinin
that sets distinct boundaries of hormonal output. Cytokinins promote the bisymmetric
distribution of the PIN-FORMED (PIN) auxin efflux proteins, which channel auxin
toward a central domain. High auxin promotes transcription of the cytokinin signaling
inhibitor AHP6, which closes the interaction loop. This bisymmetric auxin response
domain specifies the differentiation of protoxylem in a bisymmetric pattern. In
embryonic roots, cytokinin is required to translate a bisymmetric auxin response
in the cotyledons to a bisymmetric vascular pattern in the root promeristem. Conclusions:
Our results present an interactive feedback loop between hormonal signaling and
transport by which small biases in hormonal input are propagated into distinct
signaling domains to specify the vascular pattern in the root meristem. It is
an intriguing possibility that such a mechanism could transform radial patterns
and allow continuous vascular connections between other newly emerging organs.'
author:
- first_name: Anthony
full_name: Bishopp, Anthony
last_name: Bishopp
- first_name: Hanna
full_name: Help, Hanna
last_name: Help
- first_name: Sedeer
full_name: El-Showk, Sedeer
last_name: El Showk
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
- first_name: Ben
full_name: Scheres, Ben
last_name: Scheres
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Eva
full_name: Eva Benková
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Ari
full_name: Mähönen, Ari Pekka
last_name: Mähönen
- first_name: Ykä
full_name: Helariutta, Ykä
last_name: Helariutta
citation:
ama: Bishopp A, Help H, El Showk S, et al. A mutually inhibitory interaction between
auxin and cytokinin specifies vascular pattern in roots. Current Biology.
2011;21(11):917-926. doi:10.1016/j.cub.2011.04.017
apa: Bishopp, A., Help, H., El Showk, S., Weijers, D., Scheres, B., Friml, J., …
Helariutta, Y. (2011). A mutually inhibitory interaction between auxin and cytokinin
specifies vascular pattern in roots. Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2011.04.017
chicago: Bishopp, Anthony, Hanna Help, Sedeer El Showk, Dolf Weijers, Ben Scheres,
Jiří Friml, Eva Benková, Ari Mähönen, and Ykä Helariutta. “A Mutually Inhibitory
Interaction between Auxin and Cytokinin Specifies Vascular Pattern in Roots.”
Current Biology. Cell Press, 2011. https://doi.org/10.1016/j.cub.2011.04.017.
ieee: A. Bishopp et al., “A mutually inhibitory interaction between auxin
and cytokinin specifies vascular pattern in roots,” Current Biology, vol.
21, no. 11. Cell Press, pp. 917–926, 2011.
ista: Bishopp A, Help H, El Showk S, Weijers D, Scheres B, Friml J, Benková E, Mähönen
A, Helariutta Y. 2011. A mutually inhibitory interaction between auxin and cytokinin
specifies vascular pattern in roots. Current Biology. 21(11), 917–926.
mla: Bishopp, Anthony, et al. “A Mutually Inhibitory Interaction between Auxin and
Cytokinin Specifies Vascular Pattern in Roots.” Current Biology, vol. 21,
no. 11, Cell Press, 2011, pp. 917–26, doi:10.1016/j.cub.2011.04.017.
short: A. Bishopp, H. Help, S. El Showk, D. Weijers, B. Scheres, J. Friml, E. Benková,
A. Mähönen, Y. Helariutta, Current Biology 21 (2011) 917–926.
date_created: 2018-12-11T12:01:18Z
date_published: 2011-06-07T00:00:00Z
date_updated: 2021-01-12T07:40:58Z
day: '07'
doi: 10.1016/j.cub.2011.04.017
extern: 1
intvolume: ' 21'
issue: '11'
month: '06'
page: 917 - 926
publication: Current Biology
publication_status: published
publisher: Cell Press
publist_id: '3613'
quality_controlled: 0
status: public
title: A mutually inhibitory interaction between auxin and cytokinin specifies vascular
pattern in roots
type: journal_article
volume: 21
year: '2011'
...
---
_id: '3093'
abstract:
- lang: eng
text: |2-
Plants take up iron from the soil using the IRON-REGULATED TRANSPORTER 1 (IRT1) high-affinity iron transporter at the root surface. Sophisticated regulatory mechanisms allow plants to tightly control the levels of IRT1, ensuring optimal absorption of essential but toxic iron. Here, we demonstrate that overexpression of Arabidopsis thaliana IRT1 leads to constitutive IRT1 protein accumulation, metal overload, and oxidative stress. IRT1 is unexpectedly found in trans-Golgi network/early endosomes of root hair cells, and its levels and localization are unaffected by iron nutrition. Using pharmacological approaches, we show that IRT1 cycles to the plasma membrane to perform iron and metal uptake at the cell surface and is sent to the vacuole for proper turnover. We also prove that IRT1 is monoubiquitinated on several cytosol-exposed residues in vivo and that mutation of two putative monoubiquitination target residues in IRT1 triggers stabilization at the plasma membrane and leads to extreme lethality. Together, these data suggest a model in which monoubiquitin-dependent internalization/sorting and turnover keep the plasma membrane pool of IRT1 low to ensure proper iron uptake and to prevent metal toxicity. More generally, our work demonstrates the existence of monoubiquitin-dependent trafficking to lytic vacuoles in plants and points to proteasome-independent turnover of plasma membrane proteins.
author:
- first_name: Marie
full_name: Barberon, Marie
last_name: Barberon
- first_name: Enric
full_name: Zelazny, Enric
last_name: Zelazny
- first_name: Stéphanie
full_name: Robert, Stéphanie
last_name: Robert
- first_name: Geneviève
full_name: Conéjéro, Geneviève
last_name: Conéjéro
- first_name: Cathy
full_name: Curie, Cathy
last_name: Curie
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Grégory
full_name: Vert, Grégory
last_name: Vert
citation:
ama: Barberon M, Zelazny E, Robert S, et al. Monoubiquitin dependent endocytosis
of the Iron Regulated Transporter 1 IRT1 transporter controls iron uptake in plants.
PNAS. 2011;108(32):E450-E458. doi:10.1073/pnas.1100659108
apa: Barberon, M., Zelazny, E., Robert, S., Conéjéro, G., Curie, C., Friml, J.,
& Vert, G. (2011). Monoubiquitin dependent endocytosis of the Iron Regulated
Transporter 1 IRT1 transporter controls iron uptake in plants. PNAS. National
Academy of Sciences. https://doi.org/10.1073/pnas.1100659108
chicago: Barberon, Marie, Enric Zelazny, Stéphanie Robert, Geneviève Conéjéro, Cathy
Curie, Jiří Friml, and Grégory Vert. “Monoubiquitin Dependent Endocytosis of the
Iron Regulated Transporter 1 IRT1 Transporter Controls Iron Uptake in Plants.”
PNAS. National Academy of Sciences, 2011. https://doi.org/10.1073/pnas.1100659108.
ieee: M. Barberon et al., “Monoubiquitin dependent endocytosis of the Iron
Regulated Transporter 1 IRT1 transporter controls iron uptake in plants,” PNAS,
vol. 108, no. 32. National Academy of Sciences, pp. E450–E458, 2011.
ista: Barberon M, Zelazny E, Robert S, Conéjéro G, Curie C, Friml J, Vert G. 2011.
Monoubiquitin dependent endocytosis of the Iron Regulated Transporter 1 IRT1 transporter
controls iron uptake in plants. PNAS. 108(32), E450–E458.
mla: Barberon, Marie, et al. “Monoubiquitin Dependent Endocytosis of the Iron Regulated
Transporter 1 IRT1 Transporter Controls Iron Uptake in Plants.” PNAS, vol.
108, no. 32, National Academy of Sciences, 2011, pp. E450–58, doi:10.1073/pnas.1100659108.
short: M. Barberon, E. Zelazny, S. Robert, G. Conéjéro, C. Curie, J. Friml, G. Vert,
PNAS 108 (2011) E450–E458.
date_created: 2018-12-11T12:01:20Z
date_published: 2011-08-09T00:00:00Z
date_updated: 2021-01-12T07:41:00Z
day: '09'
doi: 10.1073/pnas.1100659108
extern: 1
intvolume: ' 108'
issue: '32'
month: '08'
page: E450 - E458
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '3607'
quality_controlled: 0
status: public
title: Monoubiquitin dependent endocytosis of the Iron Regulated Transporter 1 IRT1
transporter controls iron uptake in plants
type: journal_article
volume: 108
year: '2011'
...
---
_id: '3094'
abstract:
- lang: eng
text: Summary Gravitropism aligns plant growth with gravity. It involves gravity
perception and the asymmetric distribution of the phytohormone auxin. Here we
provide insights into the mechanism for hypocotyl gravitropic growth. We show
that the Arabidopsis thaliana PIN3 auxin transporter is required for the asymmetric
auxin distribution for the gravitropic response. Gravistimulation polarizes PIN3
to the bottom side of hypocotyl endodermal cells, which correlates with an increased
auxin response at the lower hypocotyl side. Both PIN3 polarization and hypocotyl
bending require the activity of the trafficking regulator GNOM and the protein
kinase PINOID. Our data suggest that gravity-induced PIN3 polarization diverts
the auxin flow to mediate the asymmetric distribution of auxin for gravitropic
shoot bending.
author:
- first_name: Hana
full_name: Rakusová, Hana
last_name: Rakusová
- first_name: Javier
full_name: Gallego-Bartolomé, Javier
last_name: Gallego Bartolomé
- first_name: Marleen
full_name: Vanstraelen, Marleen
last_name: Vanstraelen
- first_name: Hélène
full_name: Robert, Hélène S
last_name: Robert
- first_name: David
full_name: Alabadí, David
last_name: Alabadí
- first_name: Miguel
full_name: Blázquez, Miguel A
last_name: Blázquez
- first_name: Eva
full_name: Eva Benková
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Rakusová H, Gallego Bartolomé J, Vanstraelen M, et al. Polarization of PIN3
dependent auxin transport for hypocotyl gravitropic response in Arabidopsis thaliana.
Plant Journal. 2011;67(5):817-826. doi:10.1111/j.1365-313X.2011.04636.x
apa: Rakusová, H., Gallego Bartolomé, J., Vanstraelen, M., Robert, H., Alabadí,
D., Blázquez, M., … Friml, J. (2011). Polarization of PIN3 dependent auxin transport
for hypocotyl gravitropic response in Arabidopsis thaliana. Plant Journal.
Wiley-Blackwell. https://doi.org/10.1111/j.1365-313X.2011.04636.x
chicago: Rakusová, Hana, Javier Gallego Bartolomé, Marleen Vanstraelen, Hélène Robert,
David Alabadí, Miguel Blázquez, Eva Benková, and Jiří Friml. “Polarization of
PIN3 Dependent Auxin Transport for Hypocotyl Gravitropic Response in Arabidopsis
Thaliana.” Plant Journal. Wiley-Blackwell, 2011. https://doi.org/10.1111/j.1365-313X.2011.04636.x.
ieee: H. Rakusová et al., “Polarization of PIN3 dependent auxin transport
for hypocotyl gravitropic response in Arabidopsis thaliana,” Plant Journal,
vol. 67, no. 5. Wiley-Blackwell, pp. 817–826, 2011.
ista: Rakusová H, Gallego Bartolomé J, Vanstraelen M, Robert H, Alabadí D, Blázquez
M, Benková E, Friml J. 2011. Polarization of PIN3 dependent auxin transport for
hypocotyl gravitropic response in Arabidopsis thaliana. Plant Journal. 67(5),
817–826.
mla: Rakusová, Hana, et al. “Polarization of PIN3 Dependent Auxin Transport for
Hypocotyl Gravitropic Response in Arabidopsis Thaliana.” Plant Journal,
vol. 67, no. 5, Wiley-Blackwell, 2011, pp. 817–26, doi:10.1111/j.1365-313X.2011.04636.x.
short: H. Rakusová, J. Gallego Bartolomé, M. Vanstraelen, H. Robert, D. Alabadí,
M. Blázquez, E. Benková, J. Friml, Plant Journal 67 (2011) 817–826.
date_created: 2018-12-11T12:01:21Z
date_published: 2011-09-01T00:00:00Z
date_updated: 2021-01-12T07:41:01Z
day: '01'
doi: 10.1111/j.1365-313X.2011.04636.x
extern: 1
intvolume: ' 67'
issue: '5'
month: '09'
page: 817 - 826
publication: Plant Journal
publication_status: published
publisher: Wiley-Blackwell
publist_id: '3606'
quality_controlled: 0
status: public
title: Polarization of PIN3 dependent auxin transport for hypocotyl gravitropic response
in Arabidopsis thaliana
type: journal_article
volume: 67
year: '2011'
...
---
_id: '3091'
author:
- first_name: Michael
full_name: Sauer, Michael
last_name: Sauer
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Sauer M, Friml J. Fleeting hormone cues get stabilized for plant organogenesis.
Molecular Systems Biology. 2011;7. doi:10.1038/msb.2011.45
apa: Sauer, M., & Friml, J. (2011). Fleeting hormone cues get stabilized for
plant organogenesis. Molecular Systems Biology. Nature Publishing Group.
https://doi.org/10.1038/msb.2011.45
chicago: Sauer, Michael, and Jiří Friml. “Fleeting Hormone Cues Get Stabilized for
Plant Organogenesis.” Molecular Systems Biology. Nature Publishing Group,
2011. https://doi.org/10.1038/msb.2011.45.
ieee: M. Sauer and J. Friml, “Fleeting hormone cues get stabilized for plant organogenesis,”
Molecular Systems Biology, vol. 7. Nature Publishing Group, 2011.
ista: Sauer M, Friml J. 2011. Fleeting hormone cues get stabilized for plant organogenesis.
Molecular Systems Biology. 7.
mla: Sauer, Michael, and Jiří Friml. “Fleeting Hormone Cues Get Stabilized for Plant
Organogenesis.” Molecular Systems Biology, vol. 7, Nature Publishing Group,
2011, doi:10.1038/msb.2011.45.
short: M. Sauer, J. Friml, Molecular Systems Biology 7 (2011).
date_created: 2018-12-11T12:01:19Z
date_published: 2011-07-05T00:00:00Z
date_updated: 2021-01-12T07:41:00Z
day: '05'
doi: 10.1038/msb.2011.45
extern: '1'
external_id:
pmid:
- '21734646'
intvolume: ' 7'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3159970/
month: '07'
oa: 1
oa_version: Published Version
pmid: 1
publication: Molecular Systems Biology
publication_status: published
publisher: Nature Publishing Group
publist_id: '3610'
quality_controlled: '1'
status: public
title: Fleeting hormone cues get stabilized for plant organogenesis
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 7
year: '2011'
...
---
_id: '3102'
abstract:
- lang: eng
text: 'Multicellular organisms depend on cell production, cell fate specification,
and correct patterning to shape their adult body. In plants, auxin plays a prominent
role in the timely coordination of these different cellular processes. A well-studied
example is lateral root initiation, in which auxin triggers founder cell specification
and cell cycle activation of xylem pole–positioned pericycle cells. Here, we report
that the E2Fa transcription factor of Arabidopsis thaliana is an essential component
that regulates the asymmetric cell division marking lateral root initiation. Moreover,
we demonstrate that E2Fa expression is regulated by the LATERAL ORGAN BOUNDARY
DOMAIN18/LATERAL ORGAN BOUNDARY DOMAIN33 (LBD18/LBD33) dimer that is, in turn,
regulated by the auxin signaling pathway. LBD18/LBD33 mediates lateral root organogenesis
through E2Fa transcriptional activation, whereas E2Fa expression under control
of the LBD18 promoter eliminates the need for LBD18. Besides lateral root initiation,
vascular patterning is disrupted in E2Fa knockout plants, similarly as it is affected
in auxin signaling and lbd mutants, indicating that the transcriptional induction
of E2Fa through LBDs represents a general mechanism for auxin-dependent cell cycle
activation. Our data illustrate how a conserved mechanism driving cell cycle entry
has been adapted evolutionarily to connect auxin signaling with control of processes
determining plant architecture. '
author:
- first_name: Barbara
full_name: Berckmans, Barbara
last_name: Berckmans
- first_name: Valya
full_name: Vassileva, Valya
last_name: Vassileva
- first_name: Stephan
full_name: Schmid, Stephan P
last_name: Schmid
- first_name: Sara
full_name: Maes, Sara
last_name: Maes
- first_name: Boris
full_name: Parizot, Boris
last_name: Parizot
- first_name: Satoshi
full_name: Naramoto, Satoshi
last_name: Naramoto
- first_name: Zoltan
full_name: Magyar, Zoltan
last_name: Magyar
- first_name: Claire
full_name: Lessa Alvim Kamei, Claire
last_name: Lessa Alvim Kamei
- first_name: Csaba
full_name: Koncz, Csaba
last_name: Koncz
- first_name: Laszlo
full_name: Bögre, Laszlo
last_name: Bögre
- first_name: Geert
full_name: Persiau, Geert
last_name: Persiau
- first_name: Geert
full_name: De Jaeger, Geert
last_name: De Jaeger
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Rüdiger
full_name: Simon, Rüdiger
last_name: Simon
- first_name: Tom
full_name: Beeckman, Tom
last_name: Beeckman
- first_name: Lieven
full_name: de Veyldera, Lieven
last_name: De Veyldera
citation:
ama: Berckmans B, Vassileva V, Schmid S, et al. Auxin Dependent cell cycle reactivation
through transcriptional regulation of arabidopsis E2Fa by lateral organ boundary
proteins. Plant Cell. 2011;23(10):3671-3683. doi:10.1105/tpc.111.088377
apa: Berckmans, B., Vassileva, V., Schmid, S., Maes, S., Parizot, B., Naramoto,
S., … De Veyldera, L. (2011). Auxin Dependent cell cycle reactivation through
transcriptional regulation of arabidopsis E2Fa by lateral organ boundary proteins.
Plant Cell. American Society of Plant Biologists. https://doi.org/10.1105/tpc.111.088377
chicago: Berckmans, Barbara, Valya Vassileva, Stephan Schmid, Sara Maes, Boris Parizot,
Satoshi Naramoto, Zoltan Magyar, et al. “Auxin Dependent Cell Cycle Reactivation
through Transcriptional Regulation of Arabidopsis E2Fa by Lateral Organ Boundary
Proteins.” Plant Cell. American Society of Plant Biologists, 2011. https://doi.org/10.1105/tpc.111.088377.
ieee: B. Berckmans et al., “Auxin Dependent cell cycle reactivation through
transcriptional regulation of arabidopsis E2Fa by lateral organ boundary proteins,”
Plant Cell, vol. 23, no. 10. American Society of Plant Biologists, pp.
3671–3683, 2011.
ista: Berckmans B, Vassileva V, Schmid S, Maes S, Parizot B, Naramoto S, Magyar
Z, Lessa Alvim Kamei C, Koncz C, Bögre L, Persiau G, De Jaeger G, Friml J, Simon
R, Beeckman T, De Veyldera L. 2011. Auxin Dependent cell cycle reactivation through
transcriptional regulation of arabidopsis E2Fa by lateral organ boundary proteins.
Plant Cell. 23(10), 3671–3683.
mla: Berckmans, Barbara, et al. “Auxin Dependent Cell Cycle Reactivation through
Transcriptional Regulation of Arabidopsis E2Fa by Lateral Organ Boundary Proteins.”
Plant Cell, vol. 23, no. 10, American Society of Plant Biologists, 2011,
pp. 3671–83, doi:10.1105/tpc.111.088377.
short: B. Berckmans, V. Vassileva, S. Schmid, S. Maes, B. Parizot, S. Naramoto,
Z. Magyar, C. Lessa Alvim Kamei, C. Koncz, L. Bögre, G. Persiau, G. De Jaeger,
J. Friml, R. Simon, T. Beeckman, L. De Veyldera, Plant Cell 23 (2011) 3671–3683.
date_created: 2018-12-11T12:01:24Z
date_published: 2011-10-14T00:00:00Z
date_updated: 2021-01-12T07:41:04Z
day: '14'
doi: 10.1105/tpc.111.088377
extern: 1
intvolume: ' 23'
issue: '10'
month: '10'
page: 3671 - 3683
publication: Plant Cell
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '3598'
quality_controlled: 0
status: public
title: Auxin Dependent cell cycle reactivation through transcriptional regulation
of arabidopsis E2Fa by lateral organ boundary proteins
type: journal_article
volume: 23
year: '2011'
...
---
_id: '3103'
abstract:
- lang: eng
text: Endocytosis in plants has an essential role not only for basic cellular functions
but also for growth and development, hormonal signaling and communication with
the environment including nutrient delivery, toxin avoidance, and pathogen defense.
The major endocytic mechanism in plants depends on the coat protein clathrin.
It starts by clathrin-coated vesicle formation at the plasma membrane, where specific
cargoes are recognized and packaged for internalization. Recently, genetic, biochemical
and advanced microscopy studies provided initial insights into mechanisms and
roles of clathrin-mediated endocytosis in plants. Here we summarize the present
state of knowledge and compare mechanisms of clathrin-mediated endocytosis in
plants with animal and yeast paradigms as well as review plant-specific regulations
and roles of this process.
author:
- first_name: Xu
full_name: Chen, Xu
id: 4E5ADCAA-F248-11E8-B48F-1D18A9856A87
last_name: Chen
- first_name: Niloufer
full_name: Irani, Niloufer
last_name: Irani
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Chen X, Irani N, Friml J. Clathrin-mediated endocytosis: The gateway into
plant cells. Current Opinion in Plant Biology. 2011;14(6):674-682. doi:10.1016/j.pbi.2011.08.006'
apa: 'Chen, X., Irani, N., & Friml, J. (2011). Clathrin-mediated endocytosis:
The gateway into plant cells. Current Opinion in Plant Biology. Elsevier.
https://doi.org/10.1016/j.pbi.2011.08.006'
chicago: 'Chen, Xu, Niloufer Irani, and Jiří Friml. “Clathrin-Mediated Endocytosis:
The Gateway into Plant Cells.” Current Opinion in Plant Biology. Elsevier,
2011. https://doi.org/10.1016/j.pbi.2011.08.006.'
ieee: 'X. Chen, N. Irani, and J. Friml, “Clathrin-mediated endocytosis: The gateway
into plant cells,” Current Opinion in Plant Biology, vol. 14, no. 6. Elsevier,
pp. 674–682, 2011.'
ista: 'Chen X, Irani N, Friml J. 2011. Clathrin-mediated endocytosis: The gateway
into plant cells. Current Opinion in Plant Biology. 14(6), 674–682.'
mla: 'Chen, Xu, et al. “Clathrin-Mediated Endocytosis: The Gateway into Plant Cells.”
Current Opinion in Plant Biology, vol. 14, no. 6, Elsevier, 2011, pp. 674–82,
doi:10.1016/j.pbi.2011.08.006.'
short: X. Chen, N. Irani, J. Friml, Current Opinion in Plant Biology 14 (2011) 674–682.
date_created: 2018-12-11T12:01:24Z
date_published: 2011-12-01T00:00:00Z
date_updated: 2021-01-12T07:41:05Z
day: '01'
doi: 10.1016/j.pbi.2011.08.006
extern: '1'
intvolume: ' 14'
issue: '6'
language:
- iso: eng
month: '12'
oa_version: None
page: 674 - 682
publication: Current Opinion in Plant Biology
publication_status: published
publisher: Elsevier
publist_id: '3596'
quality_controlled: '1'
status: public
title: 'Clathrin-mediated endocytosis: The gateway into plant cells'
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 14
year: '2011'
...
---
_id: '3147'
abstract:
- lang: eng
text: Cancer cell of origin is difficult to identify by analyzing cells within terminal
stage tumors, whose identity could be concealed by the acquired plasticity. Thus,
an ideal approach to identify the cell of origin is to analyze proliferative abnormalities
in distinct lineages prior to malignancy. Here, we use mosaic analysis with double
markers (MADM) in mice to model gliomagenesis by initiating concurrent p53/Nf1
mutations sporadically in neural stem cells (NSCs). Surprisingly, MADM-based lineage
tracing revealed significant aberrant growth prior to malignancy only in oligodendrocyte
precursor cells (OPCs), but not in any other NSC-derived lineages or NSCs themselves.
Upon tumor formation, phenotypic and transcriptome analyses of tumor cells revealed
salient OPC features. Finally, introducing the same p53/Nf1 mutations directly
into OPCs consistently led to gliomagenesis. Our findings suggest OPCs as the
cell of origin in this model, even when initial mutations occur in NSCs, and highlight
the importance of analyzing premalignant stages to identify the cancer cell of
origin.
author:
- first_name: Chong
full_name: Liu, Chong
last_name: Liu
- first_name: Jonathan
full_name: Sage, Jonathan C
last_name: Sage
- first_name: Michael
full_name: Miller, Michael R
last_name: Miller
- first_name: Roel
full_name: Verhaak, Roel G
last_name: Verhaak
- first_name: Simon
full_name: Simon Hippenmeyer
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
- first_name: Hannes
full_name: Vogel, Hannes
last_name: Vogel
- first_name: Oded
full_name: Foreman, Oded
last_name: Foreman
- first_name: Roderick
full_name: Bronson, Roderick T
last_name: Bronson
- first_name: Akiko
full_name: Nishiyama, Akiko
last_name: Nishiyama
- first_name: Liqun
full_name: Luo, Liqun
last_name: Luo
- first_name: Hui
full_name: Zong, Hui
last_name: Zong
citation:
ama: Liu C, Sage J, Miller M, et al. Mosaic analysis with double markers reveals
tumor cell of origin in glioma. Cell. 2011;146(2):209-221. doi:10.1016/j.cell.2011.06.014
apa: Liu, C., Sage, J., Miller, M., Verhaak, R., Hippenmeyer, S., Vogel, H., … Zong,
H. (2011). Mosaic analysis with double markers reveals tumor cell of origin in
glioma. Cell. Cell Press. https://doi.org/10.1016/j.cell.2011.06.014
chicago: Liu, Chong, Jonathan Sage, Michael Miller, Roel Verhaak, Simon Hippenmeyer,
Hannes Vogel, Oded Foreman, et al. “Mosaic Analysis with Double Markers Reveals
Tumor Cell of Origin in Glioma.” Cell. Cell Press, 2011. https://doi.org/10.1016/j.cell.2011.06.014.
ieee: C. Liu et al., “Mosaic analysis with double markers reveals tumor cell
of origin in glioma,” Cell, vol. 146, no. 2. Cell Press, pp. 209–221, 2011.
ista: Liu C, Sage J, Miller M, Verhaak R, Hippenmeyer S, Vogel H, Foreman O, Bronson
R, Nishiyama A, Luo L, Zong H. 2011. Mosaic analysis with double markers reveals
tumor cell of origin in glioma. Cell. 146(2), 209–221.
mla: Liu, Chong, et al. “Mosaic Analysis with Double Markers Reveals Tumor Cell
of Origin in Glioma.” Cell, vol. 146, no. 2, Cell Press, 2011, pp. 209–21,
doi:10.1016/j.cell.2011.06.014.
short: C. Liu, J. Sage, M. Miller, R. Verhaak, S. Hippenmeyer, H. Vogel, O. Foreman,
R. Bronson, A. Nishiyama, L. Luo, H. Zong, Cell 146 (2011) 209–221.
date_created: 2018-12-11T12:01:40Z
date_published: 2011-07-22T00:00:00Z
date_updated: 2021-01-12T07:41:23Z
day: '22'
doi: 10.1016/j.cell.2011.06.014
extern: 1
intvolume: ' 146'
issue: '2'
month: '07'
page: 209 - 221
publication: Cell
publication_status: published
publisher: Cell Press
publist_id: '3548'
quality_controlled: 0
status: public
title: Mosaic analysis with double markers reveals tumor cell of origin in glioma
type: journal_article
volume: 146
year: '2011'
...