TY - JOUR
AB - Background: Murine leukemia virus (MLV) vector particles can be pseudotyped with a truncated variant of the human immunodeficiency virus type 1 (HIV-1) envelope protein (Env) and selectively target gene transfer to human cells expressing both CD4 and an appropriate co-receptor. Vector transduction mimics the HIV-1 entry process and is therefore a safe tool to study HIV-1 entry. Results: Using FLY cells, which express the MLV gag and pol genes, we generated stable producer cell lines that express the HIV-1 envelope gene and a retroviral vector genome encoding the green fluorescent protein (GFP). The BH10 or 89.6 P HIV-1 Env was expressed from a bicistronic vector which allowed the rapid selection of stable cell lines. A codon-usage-optimized synthetic env gene permitted high, Rev-independent Env expression. Vectors generated by these producer cells displayed different sensitivity to entry inhibitors. Conclusion: These data illustrate that MLV/HIV-1 vectors are a valuable screening system for entry inhibitors or neutralizing antisera generated by vaccines.
AU - Sandra Siegert
AU - Thaler, Sonja
AU - Wagner, Ralf
AU - Schnierle, Barbara S
ID - 1795
IS - 1
JF - AIDS Research and Therapy
TI - Assessment of HIV-1 entry inhibitors by MLV/HIV-1 pseudotyped vectors
VL - 2
ER -
TY - JOUR
AB -
Complex I of respiratory chains plays a central role in bioenergetics and is implicated in many human neurodegenerative diseases. An understanding of its mechanism requires a knowledge of the organization of redox centers. The arrangement of iron-sulfur clusters in the hydrophilic domain of complex I from Thermus thermophilus has been determined with the use of x-ray crystallography. One binuclear and six tetranuclear clusters are arranged, maximally 14 angstroms apart, in an 84-angstrom-long electron transfer chain. The binuclear cluster N1a and the tetranuclear cluster N7 are not in this pathway. Cluster N1a may play a role in the prevention of oxidative damage. The structure provides a framework for the interpretation of the large amounts of data accumulated on complex I.
AU - Hinchliffe, Philip
AU - Leonid Sazanov
ID - 1962
IS - 5735
JF - Science
TI - Biochemistry: Organization of iron-sulfur clusters in respiratory complex I
VL - 309
ER -
TY - BOOK
AB - Harold Davenport was one of the truly great mathematicians of the twentieth century. Based on lectures he gave at the University of Michigan in the early 1960s, this book is concerned with the use of analytic methods in the study of integer solutions to Diophantine equations and Diophantine inequalities. It provides an excellent introduction to a timeless area of number theory that is still as widely researched today as it was when the book originally appeared. The three main themes of the book are Waring's problem and the representation of integers by diagonal forms, the solubility in integers of systems of forms in many variables, and the solubility in integers of diagonal inequalities. For the second edition of the book a comprehensive foreword has been added in which three prominent authorities describe the modern context and recent developments. A thorough bibliography has also been added.
AU - Davenport, Harold
AU - Timothy Browning
ID - 210
T2 - Analytic methods for diophantine equations and diophantine inequalities
TI - Analytic methods for diophantine equations and diophantine inequalities
ER -
TY - JOUR
AB - Let f ∈ ℤ[x] be a polynomial of degree d. The paucity of non-trivial positive integer solutions to the equation f(x1)+f(x 2)=f(x3)+f(x4) is established, provided that d ≤ 7$. Also the corresponding situation is investigated for equal sums of three like polynomials.
AU - Timothy Browning
ID - 211
IS - 6
JF - Bulletin of the London Mathematical Society
TI - Equal sums of like polynomials
VL - 37
ER -
TY - JOUR
AB - For any n ≧ 2, let F ∈ ℤ [ x 1, … , xn ] be a form of degree d≧ 2, which produces a geometrically irreducible hypersurface in ℙn–1. This paper is concerned with the number N(F;B) of rational points on F = 0 which have height at most B. For any ε > 0 we establish the estimate N(F; B) = O(B n− 2+ ε ), whenever either n ≦ 5 or the hypersurface is not a union of lines. Here the implied constant depends at most upon d, n and ε.
AU - Timothy Browning
AU - Heath-Brown, Roger
ID - 212
IS - 584
JF - Journal fur die Reine und Angewandte Mathematik
TI - Counting rational points on hypersurfaces
ER -