[{"external_id":{"pmid":["20717140"]},"oa":1,"main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2924653/"}],"quality_controlled":"1","doi":"10.1038/emboj.2010.181","language":[{"iso":"eng"}],"month":"08","pmid":1,"year":"2010","publisher":"Wiley-Blackwell","publication_status":"published","author":[{"full_name":"Grunewald, Wim","last_name":"Grunewald","first_name":"Wim"},{"full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"}],"volume":29,"date_created":"2018-12-11T12:01:12Z","date_updated":"2021-01-12T07:40:51Z","publist_id":"3629","extern":"1","citation":{"chicago":"Grunewald, Wim, and Jiří Friml. “The March of the PINs: Developmental Plasticity by Dynamic Polar Targeting in Plant Cells.” EMBO Journal. Wiley-Blackwell, 2010. https://doi.org/10.1038/emboj.2010.181.","short":"W. Grunewald, J. Friml, EMBO Journal 29 (2010) 2700–2714.","mla":"Grunewald, Wim, and Jiří Friml. “The March of the PINs: Developmental Plasticity by Dynamic Polar Targeting in Plant Cells.” EMBO Journal, vol. 29, no. 16, Wiley-Blackwell, 2010, pp. 2700–14, doi:10.1038/emboj.2010.181.","ieee":"W. Grunewald and J. Friml, “The march of the PINs: Developmental plasticity by dynamic polar targeting in plant cells,” EMBO Journal, vol. 29, no. 16. Wiley-Blackwell, pp. 2700–2714, 2010.","apa":"Grunewald, W., & Friml, J. (2010). The march of the PINs: Developmental plasticity by dynamic polar targeting in plant cells. EMBO Journal. Wiley-Blackwell. https://doi.org/10.1038/emboj.2010.181","ista":"Grunewald W, Friml J. 2010. The march of the PINs: Developmental plasticity by dynamic polar targeting in plant cells. EMBO Journal. 29(16), 2700–2714.","ama":"Grunewald W, Friml J. The march of the PINs: Developmental plasticity by dynamic polar targeting in plant cells. EMBO Journal. 2010;29(16):2700-2714. doi:10.1038/emboj.2010.181"},"publication":"EMBO Journal","page":"2700 - 2714","date_published":"2010-08-18T00:00:00Z","day":"18","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","_id":"3072","intvolume":" 29","title":"The march of the PINs: Developmental plasticity by dynamic polar targeting in plant cells","status":"public","oa_version":"Published Version","type":"journal_article","issue":"16","abstract":[{"text":"Development of plants and their adaptive capacity towards ever‐changing environmental conditions largely depend on the spatial distribution of the plant hormone auxin. At the cellular level, various internal and external signals are translated into specific changes in the polar, subcellular localization of auxin transporters from the PIN family thereby directing and redirecting the intercellular fluxes of auxin. The current model of polar targeting of PIN proteins towards different plasma membrane domains encompasses apolar secretion of newly synthesized PINs followed by endocytosis and recycling back to the plasma membrane in a polarized manner. In this review, we follow the subcellular march of the PINs and highlight the cellular and molecular mechanisms behind polar foraging and subcellular trafficking pathways. Also, the entry points for different signals and regulations including by auxin itself will be discussed within the context of morphological and developmental consequences of polar targeting and subcellular trafficking.","lang":"eng"}]},{"day":"21","month":"12","doi":"10.1038/msb.2010.103","date_published":"2010-12-21T00:00:00Z","quality_controlled":0,"citation":{"ama":"Wabnik KT, Kleine Vehn J, Balla J, et al. Emergence of tissue polarization from synergy of intracellular and extracellular auxin signaling. Molecular Systems Biology. 2010;6. doi:10.1038/msb.2010.103","ista":"Wabnik KT, Kleine Vehn J, Balla J, Sauer M, Naramoto S, Reinöhl V, Merks R, Govaerts W, Friml J. 2010. Emergence of tissue polarization from synergy of intracellular and extracellular auxin signaling. Molecular Systems Biology. 6.","apa":"Wabnik, K. T., Kleine Vehn, J., Balla, J., Sauer, M., Naramoto, S., Reinöhl, V., … Friml, J. (2010). Emergence of tissue polarization from synergy of intracellular and extracellular auxin signaling. Molecular Systems Biology. Nature Publishing Group. https://doi.org/10.1038/msb.2010.103","ieee":"K. T. Wabnik et al., “Emergence of tissue polarization from synergy of intracellular and extracellular auxin signaling,” Molecular Systems Biology, vol. 6. Nature Publishing Group, 2010.","mla":"Wabnik, Krzysztof T., et al. “Emergence of Tissue Polarization from Synergy of Intracellular and Extracellular Auxin Signaling.” Molecular Systems Biology, vol. 6, Nature Publishing Group, 2010, doi:10.1038/msb.2010.103.","short":"K.T. Wabnik, J. Kleine Vehn, J. Balla, M. Sauer, S. Naramoto, V. Reinöhl, R. Merks, W. Govaerts, J. Friml, Molecular Systems Biology 6 (2010).","chicago":"Wabnik, Krzysztof T, Jürgen Kleine Vehn, Jozef Balla, Michael Sauer, Satoshi Naramoto, Vilém Reinöhl, Roeland Merks, Willy Govaerts, and Jiří Friml. “Emergence of Tissue Polarization from Synergy of Intracellular and Extracellular Auxin Signaling.” Molecular Systems Biology. Nature Publishing Group, 2010. https://doi.org/10.1038/msb.2010.103."},"publication":"Molecular Systems Biology","extern":1,"publist_id":"3622","abstract":[{"text":"Plant development is exceptionally flexible as manifested by its potential for organogenesis and regeneration, which are processes involving rearrangements of tissue polarities. Fundamental questions concern how individual cells can polarize in a coordinated manner to integrate into the multicellular context. In canalization models, the signaling molecule auxin acts as a polarizing cue, and feedback on the intercellular auxin flow is key for synchronized polarity rearrangements. We provide a novel mechanistic framework for canalization, based on up-to-date experimental data and minimal, biologically plausible assumptions. Our model combines the intracellular auxin signaling for expression of PINFORMED (PIN) auxin transporters and the theoretical postulation of extracellular auxin signaling for modulation of PIN subcellular dynamics. Computer simulations faithfully and robustly recapitulated the experimentally observed patterns of tissue polarity and asymmetric auxin distribution during formation and regeneration of vascular systems and during the competitive regulation of shoot branching by apical dominance. Additionally, our model generated new predictions that could be experimentally validated, highlighting a mechanistically conceivable explanation for the PIN polarization and canalization of the auxin flow in plants.","lang":"eng"}],"type":"journal_article","volume":6,"date_created":"2018-12-11T12:01:15Z","date_updated":"2021-01-12T07:40:54Z","author":[{"full_name":"Krzysztof Wabnik","id":"4DE369A4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7263-0560","first_name":"Krzysztof T","last_name":"Wabnik"},{"full_name":"Kleine-Vehn, Jürgen","first_name":"Jürgen","last_name":"Kleine Vehn"},{"first_name":"Jozef","last_name":"Balla","full_name":"Balla, Jozef"},{"full_name":"Sauer, Michael","first_name":"Michael","last_name":"Sauer"},{"first_name":"Satoshi","last_name":"Naramoto","full_name":"Naramoto, Satoshi"},{"last_name":"Reinöhl","first_name":"Vilém","full_name":"Reinöhl, Vilém"},{"first_name":"Roeland","last_name":"Merks","full_name":"Merks, Roeland M"},{"full_name":"Govaerts, Willy J","last_name":"Govaerts","first_name":"Willy"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","first_name":"Jirí","full_name":"Jirí Friml"}],"intvolume":" 6","publisher":"Nature Publishing Group","title":"Emergence of tissue polarization from synergy of intracellular and extracellular auxin signaling","status":"public","publication_status":"published","year":"2010","_id":"3079"},{"doi":"10.1016/j.cell.2010.09.027","date_published":"2010-10-01T00:00:00Z","quality_controlled":0,"page":"111 - 121","publication":"Cell","citation":{"ama":"Robert S, Kleine Vehn J, Barbez E, et al. ABP1 mediates auxin inhibition of clathrin-dependent endocytosis in Arabidopsis. Cell. 2010;143(1):111-121. doi:10.1016/j.cell.2010.09.027","apa":"Robert, S., Kleine Vehn, J., Barbez, E., Sauer, M., Paciorek, T., Baster, P., … Friml, J. (2010). ABP1 mediates auxin inhibition of clathrin-dependent endocytosis in Arabidopsis. Cell. Cell Press. https://doi.org/10.1016/j.cell.2010.09.027","ieee":"S. Robert et al., “ABP1 mediates auxin inhibition of clathrin-dependent endocytosis in Arabidopsis,” Cell, vol. 143, no. 1. Cell Press, pp. 111–121, 2010.","ista":"Robert S, Kleine Vehn J, Barbez E, Sauer M, Paciorek T, Baster P, Vanneste S, Zhang J, Simon S, Čovanová M, Hayashi K, Dhonukshe P, Yang Z, Bednarek S, Jones A, Luschnig C, Aniento F, Zažímalová E, Friml J. 2010. ABP1 mediates auxin inhibition of clathrin-dependent endocytosis in Arabidopsis. Cell. 143(1), 111–121.","short":"S. Robert, J. Kleine Vehn, E. Barbez, M. Sauer, T. Paciorek, P. Baster, S. Vanneste, J. Zhang, S. Simon, M. Čovanová, K. Hayashi, P. Dhonukshe, Z. Yang, S. Bednarek, A. Jones, C. Luschnig, F. Aniento, E. Zažímalová, J. Friml, Cell 143 (2010) 111–121.","mla":"Robert, Stéphanie, et al. “ABP1 Mediates Auxin Inhibition of Clathrin-Dependent Endocytosis in Arabidopsis.” Cell, vol. 143, no. 1, Cell Press, 2010, pp. 111–21, doi:10.1016/j.cell.2010.09.027.","chicago":"Robert, Stéphanie, Jürgen Kleine Vehn, Elke Barbez, Michael Sauer, Tomasz Paciorek, Pawel Baster, Steffen Vanneste, et al. “ABP1 Mediates Auxin Inhibition of Clathrin-Dependent Endocytosis in Arabidopsis.” Cell. Cell Press, 2010. https://doi.org/10.1016/j.cell.2010.09.027."},"day":"01","month":"10","date_created":"2018-12-11T12:01:13Z","date_updated":"2021-01-12T07:40:52Z","volume":143,"author":[{"full_name":"Robert, Stéphanie","first_name":"Stéphanie","last_name":"Robert"},{"full_name":"Kleine-Vehn, Jürgen","last_name":"Kleine Vehn","first_name":"Jürgen"},{"first_name":"Elke","last_name":"Barbez","full_name":"Barbez, Elke"},{"last_name":"Sauer","first_name":"Michael","full_name":"Sauer, Michael"},{"full_name":"Paciorek, Tomasz","last_name":"Paciorek","first_name":"Tomasz"},{"id":"3028BD74-F248-11E8-B48F-1D18A9856A87","last_name":"Baster","first_name":"Pawel","full_name":"Pawel Baster"},{"full_name":"Vanneste, Steffen","first_name":"Steffen","last_name":"Vanneste"},{"full_name":"Zhang, Jing","first_name":"Jing","last_name":"Zhang"},{"full_name":"Sibu Simon","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-1998-6741","first_name":"Sibu","last_name":"Simon"},{"full_name":"Čovanová, Milada","last_name":"Čovanová","first_name":"Milada"},{"full_name":"Hayashi, Kenichiro","last_name":"Hayashi","first_name":"Kenichiro"},{"full_name":"Dhonukshe, Pankaj","last_name":"Dhonukshe","first_name":"Pankaj"},{"full_name":"Yang, Zhenbiao","first_name":"Zhenbiao","last_name":"Yang"},{"first_name":"Sebastian","last_name":"Bednarek","full_name":"Bednarek, Sebastian Y"},{"full_name":"Jones, Alan M","last_name":"Jones","first_name":"Alan"},{"full_name":"Luschnig, Christian","first_name":"Christian","last_name":"Luschnig"},{"first_name":"Fernando","last_name":"Aniento","full_name":"Aniento, Fernando"},{"first_name":"Eva","last_name":"Zažímalová","full_name":"Zažímalová, Eva"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","first_name":"Jirí","full_name":"Jirí Friml"}],"publication_status":"published","status":"public","title":"ABP1 mediates auxin inhibition of clathrin-dependent endocytosis in Arabidopsis","intvolume":" 143","publisher":"Cell Press","year":"2010","_id":"3075","extern":1,"abstract":[{"lang":"eng","text":"\nSpatial distribution of the plant hormone auxin regulates multiple aspects of plant development. These self-regulating auxin gradients are established by the action of PIN auxin transporters, whose activity is regulated by their constitutive cycling between the plasma membrane and endosomes. Here, we show that auxin signaling by the auxin receptor AUXIN-BINDING PROTEIN 1 (ABP1) inhibits the clathrin-mediated internalization of PIN proteins. ABP1 acts as a positive factor in clathrin recruitment to the plasma membrane, thereby promoting endocytosis. Auxin binding to ABP1 interferes with this action and leads to the inhibition of clathrin-mediated endocytosis. Our study demonstrates that ABP1 mediates a nontranscriptional auxin signaling that regulates the evolutionarily conserved process of clathrin-mediated endocytosis and suggests that this signaling may be essential for the developmentally important feedback of auxin on its own transport."}],"publist_id":"3626","issue":"1","type":"journal_article"},{"abstract":[{"text":"Rapid advances in the field of plant biology, especially in plant cell biology, have created the need for methods that allow the localization of proteins in situ at subcellular resolution. Although in many cases recombinant proteins with fluorescent proteins can fulfill this task, antibody-based immunological detection of proteins is a complementary technique, which avoids the risk of inducing side effects by a fusion protein, such as misexpression, mistargeting, altered stability, or toxicity. Moreover, recombinant protein techniques are applicable only to a rather limited set of model plants. The immunolocalization protocols presented here can be used to display protein localization patterns in different tissues of various plant species. This chapter describes a whole mount immunolocalization protocol, which has been extensively used in Arabidopsis roots and some above-ground tissues, and that also works in other species. Additionally, for bulky or hard tissue types, a variation of this protocol for paraffin-embedded sections is given.","lang":"eng"}],"publist_id":"3623","extern":1,"type":"book_chapter","alternative_title":["Methods in Molecular Biology"],"author":[{"first_name":"Michael","last_name":"Sauer","full_name":"Sauer, Michael"},{"full_name":"Jirí Friml","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","first_name":"Jirí"}],"date_created":"2018-12-11T12:01:14Z","date_updated":"2021-01-12T07:40:53Z","volume":655,"_id":"3078","year":"2010","status":"public","title":"Immunolocalization of proteins in plants ","publication_status":"published","publisher":"Humana Press","intvolume":" 655","editor":[{"full_name":"Hennig, Lars","last_name":"Hennig","first_name":"Lars"},{"full_name":"Köhler, Claudia","last_name":"Köhler","first_name":"Claudia"}],"month":"08","day":"12","date_published":"2010-08-12T00:00:00Z","doi":"10.1007/978-1-60761-765-5_17","publication":"Plant Developmental Biology","citation":{"ama":"Sauer M, Friml J. Immunolocalization of proteins in plants . In: Hennig L, Köhler C, eds. Plant Developmental Biology. Vol 655. Humana Press; 2010:253-263. doi:10.1007/978-1-60761-765-5_17","ieee":"M. Sauer and J. Friml, “Immunolocalization of proteins in plants ,” in Plant Developmental Biology, vol. 655, L. Hennig and C. Köhler, Eds. Humana Press, 2010, pp. 253–263.","apa":"Sauer, M., & Friml, J. (2010). Immunolocalization of proteins in plants . In L. Hennig & C. Köhler (Eds.), Plant Developmental Biology (Vol. 655, pp. 253–263). Humana Press. https://doi.org/10.1007/978-1-60761-765-5_17","ista":"Sauer M, Friml J. 2010.Immunolocalization of proteins in plants . In: Plant Developmental Biology. Methods in Molecular Biology, vol. 655, 253–263.","short":"M. Sauer, J. Friml, in:, L. Hennig, C. Köhler (Eds.), Plant Developmental Biology, Humana Press, 2010, pp. 253–263.","mla":"Sauer, Michael, and Jiří Friml. “Immunolocalization of Proteins in Plants .” Plant Developmental Biology, edited by Lars Hennig and Claudia Köhler, vol. 655, Humana Press, 2010, pp. 253–63, doi:10.1007/978-1-60761-765-5_17.","chicago":"Sauer, Michael, and Jiří Friml. “Immunolocalization of Proteins in Plants .” In Plant Developmental Biology, edited by Lars Hennig and Claudia Köhler, 655:253–63. Humana Press, 2010. https://doi.org/10.1007/978-1-60761-765-5_17."},"quality_controlled":0,"page":"253 - 263"},{"month":"08","day":"01","date_published":"2010-08-01T00:00:00Z","doi":"10.1105/tpc.110.075424","publication":"Plant Cell","citation":{"mla":"Feraru, Elena, et al. “The AP 3 β Adaptin Mediates the Biogenesis and Function of Lytic Vacuoles in Arabidopsis.” Plant Cell, vol. 22, no. 8, American Society of Plant Biologists, 2010, pp. 2812–24, doi:10.1105/tpc.110.075424.","short":"E. Feraru, T. Paciorek, M. Feraru, M. Zwiewka, R. De Groodt, R. De Rycke, J. Kleine Vehn, J. Friml, Plant Cell 22 (2010) 2812–2824.","chicago":"Feraru, Elena, Tomasz Paciorek, Mugurel Feraru, Marta Zwiewka, Ruth De Groodt, Riet De Rycke, Jürgen Kleine Vehn, and Jiří Friml. “The AP 3 β Adaptin Mediates the Biogenesis and Function of Lytic Vacuoles in Arabidopsis.” Plant Cell. American Society of Plant Biologists, 2010. https://doi.org/10.1105/tpc.110.075424.","ama":"Feraru E, Paciorek T, Feraru M, et al. The AP 3 β adaptin mediates the biogenesis and function of lytic vacuoles in Arabidopsis. Plant Cell. 2010;22(8):2812-2824. doi:10.1105/tpc.110.075424","ista":"Feraru E, Paciorek T, Feraru M, Zwiewka M, De Groodt R, De Rycke R, Kleine Vehn J, Friml J. 2010. The AP 3 β adaptin mediates the biogenesis and function of lytic vacuoles in Arabidopsis. Plant Cell. 22(8), 2812–2824.","ieee":"E. Feraru et al., “The AP 3 β adaptin mediates the biogenesis and function of lytic vacuoles in Arabidopsis,” Plant Cell, vol. 22, no. 8. American Society of Plant Biologists, pp. 2812–2824, 2010.","apa":"Feraru, E., Paciorek, T., Feraru, M., Zwiewka, M., De Groodt, R., De Rycke, R., … Friml, J. (2010). The AP 3 β adaptin mediates the biogenesis and function of lytic vacuoles in Arabidopsis. Plant Cell. American Society of Plant Biologists. https://doi.org/10.1105/tpc.110.075424"},"quality_controlled":0,"page":"2812 - 2824","abstract":[{"text":"Plant vacuoles are essential multifunctional organelles largely distinct from similar organelles in other eukaryotes. Embryo protein storage vacuoles and the lytic vacuoles that perform a general degradation function are the best characterized, but little is known about the biogenesis and transition between these vacuolar types. Here, we designed a fluorescent marker- based forward genetic screen in Arabidopsis thaliana and identified a protein affected trafficking2 (pat2) mutant, whose lytic vacuoles display altered morphology and accumulation of proteins. Unlike other mutants affecting the vacuole, pat2 is specifically defective in the biogenesis, identity, and function of lytic vacuoles but shows normal sorting of proteins to storage vacuoles. PAT2 encodes a putative β-subunit of adaptor protein complex 3 (AP-3) that can partially complement the corresponding yeast mutant. Manipulations of the putative AP-3 β adaptin functions suggest a plant-specific role for the evolutionarily conserved AP-3 β in mediating lytic vacuole performance and transition of storage into the lytic vacuoles independently of the main prevacuolar compartment-based trafficking route.","lang":"eng"}],"issue":"8","publist_id":"3630","extern":1,"type":"journal_article","author":[{"full_name":"Feraru, Elena","last_name":"Feraru","first_name":"Elena"},{"full_name":"Paciorek, Tomasz","last_name":"Paciorek","first_name":"Tomasz"},{"first_name":"Mugurel","last_name":"Feraru","full_name":"Feraru, Mugurel I"},{"full_name":"Zwiewka, Marta","last_name":"Zwiewka","first_name":"Marta"},{"first_name":"Ruth","last_name":"De Groodt","full_name":"De Groodt, Ruth"},{"full_name":"De Rycke, Riet M","first_name":"Riet","last_name":"De Rycke"},{"last_name":"Kleine Vehn","first_name":"Jürgen","full_name":"Kleine-Vehn, Jürgen"},{"first_name":"Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Jirí Friml"}],"date_updated":"2021-01-12T07:40:51Z","date_created":"2018-12-11T12:01:12Z","volume":22,"_id":"3071","year":"2010","publication_status":"published","title":"The AP 3 β adaptin mediates the biogenesis and function of lytic vacuoles in Arabidopsis","status":"public","publisher":"American Society of Plant Biologists","intvolume":" 22"}]