@inbook{7743, abstract = {Experimental studies have demonstrated that environmental variation can create genotype‐environment interactions (GEIs) in the traits involved in sexual selection. Understanding the genetic architecture of phenotype across environments will require statistical tests that can describe both changes in genetic variance and covariance across environments. This chapter outlines the theoretical framework for the processes of sexual selection in the wild, identifying key parameters in wild systems, and highlighting the potential effects of the environment. It describes the proposed approaches for the estimation of these key parameters in a quantitative genetic framework within naturally occurring pedigreed populations. The chapter provides a worked example for a range of analysis methods. It aims to provide an overview of the analytical methods that can be used to model GEIs for traits involved in sexual selection in naturally occurring pedigreed populations.}, author = {Robinson, Matthew Richard and Qvarnström, Anna}, booktitle = {Genotype-by-Environment Interactions and Sexual Selection}, editor = {Hunt, John and Hosken, David}, isbn = {9780470671795}, pages = {137--168}, publisher = {Wiley}, title = {{Influence of the environment on the genetic architecture of traits involved in sexual selection within wild populations}}, doi = {10.1002/9781118912591.ch6}, year = {2014}, } @article{7744, author = {Robinson, Matthew Richard and Wray, Naomi R. and Visscher, Peter M.}, issn = {0168-9525}, journal = {Trends in Genetics}, number = {4}, pages = {124--132}, publisher = {Elsevier}, title = {{Explaining additional genetic variation in complex traits}}, doi = {10.1016/j.tig.2014.02.003}, volume = {30}, year = {2014}, } @article{7768, abstract = {We investigate the vibrational modes of quasi-two-dimensional disordered colloidal packings of hard colloidal spheres with short-range attractions as a function of packing fraction. Certain properties of the vibrational density of states (vDOS) are shown to correlate with the density and structure of the samples (i.e., in sparsely versus densely packed samples). Specifically, a crossover from dense glassy to sparse gel-like states is suggested by an excess of phonon modes at low frequency and by a variation in the slope of the vDOS with frequency at low frequency. This change in phonon mode distribution is demonstrated to arise largely from localized vibrations that involve individual and/or small clusters of particles with few local bonds. Conventional order parameters and void statistics did not exhibit obvious gel-glass signatures as a function of volume fraction. These mode behaviors and accompanying structural insights offer a potentially new set of indicators for identification of glass-gel transitions and for assignment of gel-like versus glass-like character to a disordered solid material.}, author = {Lohr, Matthew A. and Still, Tim and Ganti, Raman and Gratale, Matthew D. and Davidson, Zoey S. and Aptowicz, Kevin B. and Goodrich, Carl Peter and Sussman, Daniel M. and Yodh, A. G.}, issn = {1539-3755}, journal = {Physical Review E}, number = {6}, publisher = {American Physical Society}, title = {{Vibrational and structural signatures of the crossover between dense glassy and sparse gel-like attractive colloidal packings}}, doi = {10.1103/physreve.90.062305}, volume = {90}, year = {2014}, } @article{7771, abstract = {In their Letter, Schreck, Bertrand, O'Hern and Shattuck [Phys. Rev. Lett. 107, 078301 (2011)] study nonlinearities in jammed particulate systems that arise when contacts are altered. They conclude that there is "no harmonic regime in the large system limit for all compressions" and "at jamming onset for any system size." Their argument rests on the claim that for finite-range repulsive potentials, of the form used in studies of jamming, the breaking or forming of a single contact is sufficient to destroy the linear regime. We dispute these conclusions and argue that linear response is both justified and essential for understanding the nature of the jammed solid. }, author = {Goodrich, Carl Peter and Liu, Andrea J. and Nagel, Sidney R.}, issn = {0031-9007}, journal = {Physical Review Letters}, number = {4}, publisher = {American Physical Society}, title = {{Comment on “Repulsive contact interactions make jammed particulate systems inherently nonharmonic”}}, doi = {10.1103/physrevlett.112.049801}, volume = {112}, year = {2014}, } @article{7772, abstract = {Particle tracking and displacement covariance matrix techniques are employed to investigate the phonon dispersion relations of two-dimensional colloidal glasses composed of soft, thermoresponsive microgel particles whose temperature-sensitive size permits in situ variation of particle packing fraction. Bulk, B, and shear, G, moduli of the colloidal glasses are extracted from the dispersion relations as a function of packing fraction, and variation of the ratio G/B with packing fraction is found to agree quantitatively with predictions for jammed packings of frictional soft particles. In addition, G and B individually agree with numerical predictions for frictional particles. This remarkable level of agreement enabled us to extract an energy scale for the interparticle interaction from the individual elastic constants and to derive an approximate estimate for the interparticle friction coefficient.}, author = {Still, Tim and Goodrich, Carl Peter and Chen, Ke and Yunker, Peter J. and Schoenholz, Samuel and Liu, Andrea J. and Yodh, A. G.}, issn = {1539-3755}, journal = {Physical Review E}, number = {1}, publisher = {American Physical Society}, title = {{Phonon dispersion and elastic moduli of two-dimensional disordered colloidal packings of soft particles with frictional interactions}}, doi = {10.1103/physreve.89.012301}, volume = {89}, year = {2014}, } @article{7773, abstract = {For more than a century, physicists have described real solids in terms of perturbations about perfect crystalline order1. Such an approach takes us only so far: a glass, another ubiquitous form of rigid matter, cannot be described in any meaningful sense as a defected crystal2. Is there an opposite extreme to a crystal—a solid with complete disorder—that forms an alternative starting point for understanding real materials? Here, we argue that the solid comprising particles with finite-ranged interactions at the jamming transition3,4,5 constitutes such a limit. It has been shown that the physics associated with this transition can be extended to interactions that are long ranged6. We demonstrate that jamming physics is not restricted to amorphous systems, but dominates the behaviour of solids with surprisingly high order. Just as the free-electron and tight-binding models represent two idealized cases from which to understand electronic structure1, we identify two extreme limits of mechanical behaviour. Thus, the physics of jamming can be set side by side with the physics of crystals to provide an organizing structure for understanding the mechanical properties of solids over the entire spectrum of disorder.}, author = {Goodrich, Carl Peter and Liu, Andrea J. and Nagel, Sidney R.}, issn = {1745-2473}, journal = {Nature Physics}, number = {8}, pages = {578--581}, publisher = {Springer Nature}, title = {{Solids between the mechanical extremes of order and disorder}}, doi = {10.1038/nphys3006}, volume = {10}, year = {2014}, } @article{7769, abstract = {Athermal packings of soft repulsive spheres exhibit a sharp jamming transition in the thermodynamic limit. Upon further compression, various structural and mechanical properties display clean power-law behavior over many decades in pressure. As with any phase transition, the rounding of such behavior in finite systems close to the transition plays an important role in understanding the nature of the transition itself. The situation for jamming is surprisingly rich: the assumption that jammed packings are isotropic is only strictly true in the large-size limit, and finite-size has a profound effect on the very meaning of jamming. Here, we provide a comprehensive numerical study of finite-size effects in sphere packings above the jamming transition, focusing on stability as well as the scaling of the contact number and the elastic response.}, author = {Goodrich, Carl Peter and Dagois-Bohy, Simon and Tighe, Brian P. and van Hecke, Martin and Liu, Andrea J. and Nagel, Sidney R.}, issn = {1539-3755}, journal = {Physical Review E}, number = {2}, publisher = {American Physical Society}, title = {{Jamming in finite systems: Stability, anisotropy, fluctuations, and scaling}}, doi = {10.1103/physreve.90.022138}, volume = {90}, year = {2014}, } @article{7770, abstract = {Packings of frictionless athermal particles that interact only when they overlap experience a jamming transition as a function of packing density. Such packings provide the foundation for the theory of jamming. This theory rests on the observation that, despite the multitude of disordered configurations, the mechanical response to linear order depends only on the distance to the transition. We investigate the validity and utility of such measurements that invoke the harmonic approximation and show that, despite particles coming in and out of contact, there is a well-defined linear regime in the thermodynamic limit.}, author = {Goodrich, Carl Peter and Liu, Andrea J. and Nagel, Sidney R.}, issn = {1539-3755}, journal = {Physical Review E}, number = {2}, publisher = {American Physical Society}, title = {{Contact nonlinearities and linear response in jammed particulate packings}}, doi = {10.1103/physreve.90.022201}, volume = {90}, year = {2014}, } @article{8021, abstract = {Most excitatory inputs in the mammalian brain are made on dendritic spines, rather than on dendritic shafts. Spines compartmentalize calcium, and this biochemical isolation can underlie input-specific synaptic plasticity, providing a raison d'etre for spines. However, recent results indicate that the spine can experience a membrane potential different from that in the parent dendrite, as though the spine neck electrically isolated the spine. Here we use two-photon calcium imaging of mouse neocortical pyramidal neurons to analyze the correlation between the morphologies of spines activated under minimal synaptic stimulation and the excitatory postsynaptic potentials they generate. We find that excitatory postsynaptic potential amplitudes are inversely correlated with spine neck lengths. Furthermore, a spike timing-dependent plasticity protocol, in which two-photon glutamate uncaging over a spine is paired with postsynaptic spikes, produces rapid shrinkage of the spine neck and concomitant increases in the amplitude of the evoked spine potentials. Using numerical simulations, we explore the parameter regimes for the spine neck resistance and synaptic conductance changes necessary to explain our observations. Our data, directly correlating synaptic and morphological plasticity, imply that long-necked spines have small or negligible somatic voltage contributions, but that, upon synaptic stimulation paired with postsynaptic activity, they can shorten their necks and increase synaptic efficacy, thus changing the input/output gain of pyramidal neurons. }, author = {Araya, R. and Vogels, Tim P and Yuste, R.}, issn = {1091-6490}, journal = {Proceedings of the National Academy of Sciences}, number = {28}, pages = {E2895--E2904}, publisher = {Proceedings of the National Academy of Sciences}, title = {{Activity-dependent dendritic spine neck changes are correlated with synaptic strength}}, doi = {10.1073/pnas.1321869111}, volume = {111}, year = {2014}, } @article{8023, abstract = {Uniform random sparse network architectures are ubiquitous in computational neuroscience, but the implicit hypothesis that they are a good representation of real neuronal networks has been met with skepticism. Here we used two experimental data sets, a study of triplet connectivity statistics and a data set measuring neuronal responses to channelrhodopsin stimuli, to evaluate the fidelity of thousands of model networks. Network architectures comprised three neuron types (excitatory, fast spiking, and nonfast spiking inhibitory) and were created from a set of rules that govern the statistics of the resulting connection types. In a high-dimensional parameter scan, we varied the degree distributions (i.e., how many cells each neuron connects with) and the synaptic weight correlations of synapses from or onto the same neuron. These variations converted initially uniform random and homogeneously connected networks, in which every neuron sent and received equal numbers of synapses with equal synaptic strength distributions, to highly heterogeneous networks in which the number of synapses per neuron, as well as average synaptic strength of synapses from or to a neuron were variable. By evaluating the impact of each variable on the network structure and dynamics, and their similarity to the experimental data, we could falsify the uniform random sparse connectivity hypothesis for 7 of 36 connectivity parameters, but we also confirmed the hypothesis in 8 cases. Twenty-one parameters had no substantial impact on the results of the test protocols we used.}, author = {Tomm, Christian and Avermann, Michael and Petersen, Carl and Gerstner, Wulfram and Vogels, Tim P}, issn = {1522-1598}, journal = {Journal of Neurophysiology}, number = {8}, pages = {1801--1814}, publisher = {American Physiological Society}, title = {{Connection-type-specific biases make uniform random network models consistent with cortical recordings}}, doi = {10.1152/jn.00629.2013}, volume = {112}, year = {2014}, } @article{8022, abstract = {Populations of neurons in motor cortex engage in complex transient dynamics of large amplitude during the execution of limb movements. Traditional network models with stochastically assigned synapses cannot reproduce this behavior. Here we introduce a class of cortical architectures with strong and random excitatory recurrence that is stabilized by intricate, fine-tuned inhibition, optimized from a control theory perspective. Such networks transiently amplify specific activity states and can be used to reliably execute multidimensional movement patterns. Similar to the experimental observations, these transients must be preceded by a steady-state initialization phase from which the network relaxes back into the background state by way of complex internal dynamics. In our networks, excitation and inhibition are as tightly balanced as recently reported in experiments across several brain areas, suggesting inhibitory control of complex excitatory recurrence as a generic organizational principle in cortex.}, author = {Hennequin, Guillaume and Vogels, Tim P and Gerstner, Wulfram}, issn = {0896-6273}, journal = {Neuron}, number = {6}, pages = {1394--1406}, publisher = {Elsevier}, title = {{Optimal control of transient dynamics in balanced networks supports generation of complex movements}}, doi = {10.1016/j.neuron.2014.04.045}, volume = {82}, year = {2014}, } @article{809, abstract = {The assembly of HIV-1 is mediated by oligomerization of the major structural polyprotein, Gag, into a hexameric protein lattice at the plasma membrane of the infected cell. This leads to budding and release of progeny immature virus particles. Subsequent proteolytic cleavage of Gag triggers rearrangement of the particles to form mature infectious virions. Obtaining a structural model of the assembled lattice of Gag within immature virus particles is necessary to understand the interactions that mediate assembly of HIV-1 particles in the infected cell, and to describe the substrate that is subsequently cleaved by the viral protease. An 8-Å resolution structure of an immature virus-like tubular array assembled from a Gag-derived protein of the related retrovirus Mason-Pfizer monkey virus (M-PMV) has previously been reported, and a model for the arrangement of the HIV-1 capsid (CA) domains has been generated based on homology to this structure. Here we have assembled tubular arrays of a HIV-1 Gag-derived protein with an immature-like arrangement of the C-terminal CA domains and have solved their structure by using hybrid cryo-EM and tomography analysis. The structure reveals the arrangement of the C-terminal domain of CA within an immature-like HIV-1 Gag lattice, and provides, to our knowledge, the first high-resolution view of the region immediately downstream of CA, which is essential for assembly, and is significantly different from the respective region in M-PMV. Our results reveal a hollow column of density for this region in HIV-1 that is compatible with the presence of a six-helix bundle at this position.}, author = {Bharata, Tanmay A and Menendez, Luis R and Hagena, Wim J and Luxd, Vanda and Igonete, Sebastien and Schorba, Martin and Florian Schur and Kraüsslich, Hans Georg and Briggsa, John A}, journal = {PNAS}, number = {22}, pages = {8233 -- 8238}, publisher = {National Academy of Sciences}, title = {{Cryo electron microscopy of tubular arrays of HIV-1 Gag resolves structures essential for immature virus assembly}}, doi = {10.1073/pnas.1401455111}, volume = {111}, year = {2014}, } @article{8244, abstract = {Passive immunotherapy with monoclonal antibodies represents a cornerstone of human anticancer therapies, but has not been established in veterinary medicine yet. As the tumor-associated antigen EGFR (ErbB-1) is highly conserved between humans and dogs, and considering the effectiveness of the anti-EGFR antibody cetuximab in human clinical oncology, we present here a “caninized” version of this antibody, can225IgG, for comparative oncology studies. Variable region genes of 225, the murine precursor of cetuximab, were fused with canine constant heavy gamma and kappa chain genes, respectively, and transfected into Chinese hamster ovary (CHO) DUKX-B11 cells. Of note, 480 clones were screened and the best clones were selected according to productivity and highest specificity in EGFR-coated ELISA. Upon purification with Protein G, the recombinant cetuximab-like canine IgG was tested for integrity, correct assembly, and functionality. Specific binding to the surface of EGFR-overexpressing cells was assessed by flow cytometry and immunofluorescence; moreover, binding to canine mammary tissue was demonstrated by immunohistochemistry. In cell viability and proliferation assays, incubation with can225IgG led to significant tumor cell growth inhibition. Moreover, this antibody mediated significant tumor cell killing via phagocytosis in vitro. We thus present here, for the first time, the generation of a canine IgG antibody and its hypothetical structure. On the basis of its cetuximab-like binding site, on the one hand, and the expression of a 91% homologous EGFR molecule in canine cancer, on the other hand, this antibody may be a promising research compound to establish passive immunotherapy in dog patients with cancer.}, author = {Singer, J. and Fazekas, Judit and Wang, W. and Weichselbaumer, M. and Matz, M. and Mader, A. and Steinfellner, W. and Meitz, S. and Mechtcheriakova, D. and Sobanov, Y. and Willmann, M. and Stockner, T. and Spillner, E. and Kunert, R. and Jensen-Jarolim, E.}, issn = {1535-7163}, journal = {Molecular Cancer Therapeutics}, number = {7}, pages = {1777--1790}, publisher = {American Association for Cancer Research}, title = {{Generation of a canine anti-EGFR (ErbB-1) antibody for passive immunotherapy in dog cancer patients}}, doi = {10.1158/1535-7163.mct-13-0288}, volume = {13}, year = {2014}, } @article{8459, abstract = {Nuclear magnetic resonance (NMR) is a powerful tool for observing the motion of biomolecules at the atomic level. One technique, the analysis of relaxation dispersion phenomenon, is highly suited for studying the kinetics and thermodynamics of biological processes. Built on top of the relax computational environment for NMR dynamics is a new dispersion analysis designed to be comprehensive, accurate and easy-to-use. The software supports more models, both numeric and analytic, than current solutions. An automated protocol, available for scripting and driving the graphical user interface (GUI), is designed to simplify the analysis of dispersion data for NMR spectroscopists. Decreases in optimization time are granted by parallelization for running on computer clusters and by skipping an initial grid search by using parameters from one solution as the starting point for another —using analytic model results for the numeric models, taking advantage of model nesting, and using averaged non-clustered results for the clustered analysis.}, author = {Morin, Sébastien and Linnet, Troels E and Lescanne, Mathilde and Schanda, Paul and Thompson, Gary S and Tollinger, Martin and Teilum, Kaare and Gagné, Stéphane and Marion, Dominique and Griesinger, Christian and Blackledge, Martin and d’Auvergne, Edward J}, issn = {1367-4803}, journal = {Bioinformatics}, keywords = {Statistics and Probability, Computational Theory and Mathematics, Biochemistry, Molecular Biology, Computational Mathematics, Computer Science Applications}, number = {15}, pages = {2219--2220}, publisher = {Oxford University Press}, title = {{Relax: The analysis of biomolecular kinetics and thermodynamics using NMR relaxation dispersion data}}, doi = {10.1093/bioinformatics/btu166}, volume = {30}, year = {2014}, } @article{8458, abstract = {The maintenance of bacterial cell shape and integrity is largely attributed to peptidoglycan, a highly cross-linked biopolymer. The transpeptidases that perform this cross-linking are important targets for antibiotics. Despite this biomedical importance, to date no structure of a protein in complex with an intact bacterial peptidoglycan has been resolved, primarily due to the large size and flexibility of peptidoglycan sacculi. Here we use solid-state NMR spectroscopy to derive for the first time an atomic model of an l,d-transpeptidase from Bacillus subtilis bound to its natural substrate, the intact B. subtilis peptidoglycan. Importantly, the model obtained from protein chemical shift perturbation data shows that both domains—the catalytic domain as well as the proposed peptidoglycan recognition domain—are important for the interaction and reveals a novel binding motif that involves residues outside of the classical enzymatic pocket. Experiments on mutants and truncated protein constructs independently confirm the binding site and the implication of both domains. Through measurements of dipolar-coupling derived order parameters of bond motion we show that protein binding reduces the flexibility of peptidoglycan. This first report of an atomic model of a protein–peptidoglycan complex paves the way for the design of new antibiotic drugs targeting l,d-transpeptidases. The strategy developed here can be extended to the study of a large variety of enzymes involved in peptidoglycan morphogenesis.}, author = {Schanda, Paul and Triboulet, Sébastien and Laguri, Cédric and Bougault, Catherine M. and Ayala, Isabel and Callon, Morgane and Arthur, Michel and Simorre, Jean-Pierre}, issn = {0002-7863}, journal = {Journal of the American Chemical Society}, number = {51}, pages = {17852--17860}, publisher = {American Chemical Society}, title = {{Atomic model of a cell-wall cross-linking enzyme in complex with an intact bacterial peptidoglycan}}, doi = {10.1021/ja5105987}, volume = {136}, year = {2014}, } @article{8460, abstract = {The function of proteins depends on their ability to sample a variety of states differing in structure and free energy. Deciphering how the various thermally accessible conformations are connected, and understanding their structures and relative energies is crucial in rationalizing protein function. Many biomolecular reactions take place within microseconds to milliseconds, and this timescale is therefore of central functional importance. Here we show that R1ρ relaxation dispersion experiments in magic‐angle‐spinning solid‐state NMR spectroscopy make it possible to investigate the thermodynamics and kinetics of such exchange process, and gain insight into structural features of short‐lived states.}, author = {Ma, Peixiang and Haller, Jens D. and Zajakala, Jérémy and Macek, Pavel and Sivertsen, Astrid C. and Willbold, Dieter and Boisbouvier, Jérôme and Schanda, Paul}, issn = {1433-7851}, journal = {Angewandte Chemie International Edition}, number = {17}, pages = {4312--4317}, publisher = {Wiley}, title = {{Probing transient conformational states of proteins by solid-state R1ρ relaxation-dispersion NMR spectroscopy}}, doi = {10.1002/anie.201311275}, volume = {53}, year = {2014}, } @article{8501, abstract = {In this paper, we study small perturbations of a class of non-convex integrable Hamiltonians with two degrees of freedom, and we prove a result of diffusion for an open and dense set of perturbations, with an optimal time of diffusion which grows linearly with respect to the inverse of the size of the perturbation.}, author = {Bounemoura, Abed and Kaloshin, Vadim}, issn = {1609-3321}, journal = {Moscow Mathematical Journal}, keywords = {General Mathematics}, number = {2}, pages = {181--203}, publisher = {Independent University of Moscow}, title = {{Generic fast diffusion for a class of non-convex Hamiltonians with two degrees of freedom}}, doi = {10.17323/1609-4514-2014-14-2-181-203}, volume = {14}, year = {2014}, } @article{8500, abstract = {The main model studied in this paper is a lattice of pendula with a nearest‐neighbor coupling. If the coupling is weak, then the system is near‐integrable and KAM tori fill most of the phase space. For all KAM trajectories the energy of each pendulum stays within a narrow band for all time. Still, we show that for an arbitrarily weak coupling of a certain localized type, the neighboring pendula can exchange energy. In fact, the energy can be transferred between the pendula in any prescribed way.}, author = {Kaloshin, Vadim and Levi, Mark and Saprykina, Maria}, issn = {0010-3640}, journal = {Communications on Pure and Applied Mathematics}, keywords = {Applied Mathematics, General Mathematics}, number = {5}, pages = {748--775}, publisher = {Wiley}, title = {{Arnol′d diffusion in a pendulum lattice}}, doi = {10.1002/cpa.21509}, volume = {67}, year = {2014}, } @article{852, abstract = {Rapid divergence of gene copies after duplication is thought to determine the fate of the copies and evolution of novel protein functions. However, data on howlong the gene copies continue to experience an elevated rate of evolution remain scarce. Standard theory of gene duplications based on some level of genetic redundancy of gene copies predicts that the period of accelerated evolutionmust end relatively quickly. Using a maximum-likelihood approach we estimate preduplication, initial postduplication, and recent postduplication rates of evolution that occurred in themammalian lineage.Wefind that both gene copies experience a similar in magnitude acceleration in their rate of evolution. The copy located in the original genomic position typically returns to the preduplication rates of evolution in a short period of time. The burst of faster evolution of the copy that is located in a new genomic position typically lasts longer. Furthermore, the fast-evolving copies on average continue to evolve faster than the preduplication rates far longer than predicted by standard theory of gene duplications.We hypothesize that the prolonged elevated rates of evolution are determined by functional properties that were acquired during, or soon after, the gene duplication event. }, author = {Rosello, Oriol P and Fyodor Kondrashov}, journal = {Genome Biology and Evolution}, number = {8}, pages = {1949 -- 1955}, publisher = {Oxford University Press}, title = {{Long-Term asymmetrical acceleration of protein evolution after gene duplication}}, doi = {10.1093/gbe/evu159}, volume = {6}, year = {2014}, } @article{856, abstract = {The emergence of new genes throughout evolution requires rewiring and extension of regulatory networks. However, the molecular details of how the transcriptional regulation of new gene copies evolves remain largely unexplored. Here we show how duplication of a transcription factor gene allowed the emergence of two independent regulatory circuits. Interestingly, the ancestral transcription factor was promiscuous and could bind different motifs in its target promoters. After duplication, one paralogue evolved increased binding specificity so that it only binds one type of motif, whereas the other copy evolved a decreased activity so that it only activates promoters that contain multiple binding sites. Interestingly, only a few mutations in both the DNA-binding domains and in the promoter binding sites were required to gradually disentangle the two networks. These results reveal how duplication of a promiscuous transcription factor followed by concerted cis and trans mutations allows expansion of a regulatory network.}, author = {Pougach, Ksenia S and Voet, Arnout R and Fyodor Kondrashov and Voordeckers, Karin and Christiaens, Joaquin F and Baying, Bianka and Bénès, Vladimı́r and Sakai, Ryo and Aerts, Jan A and Zhu, Bo and Van Dijck, Patrick and Verstrepen, Kevin J}, journal = {Nature Communications}, publisher = {Nature Publishing Group}, title = {{Duplication of a promiscuous transcription factor drives the emergence of a new regulatory network}}, doi = {10.1038/ncomms5868}, volume = {5}, year = {2014}, }