---
_id: '2945'
abstract:
- lang: eng
text: In search of foreign antigens, lymphocytes recirculate from the blood, through
lymph nodes, into lymphatics and back to the blood. Dendritic cells also migrate
to lymph nodes for optimal interaction with lymphocytes. This continuous trafficking
of immune cells into and out of lymph nodes is essential for immune surveillance
of foreign invaders. In this article, we review our current understanding of the
functions of high endothelial venules (HEVs), stroma and lymphatics in the entry,
positioning and exit of immune cells in lymph nodes during homeostasis, and we
highlight the unexpected role of dendritic cells in the control of lymphocyte
homing through HEVs.
acknowledgement: We thank M. Sixt and A. Peixoto for helpful comments on the manuscript.
Work in the laboratory of J.-P.G. is supported by grants from Fondation ARC pour
la Recherche sur le Cancer, Agence Nationale de la Recherche (ANR), Institut National
du Cancer (INCA), Fondation RITC and Région Midi-Pyrénées. Research by R.F. is supported
by Deutsche Forschungsgemeinschaft (DFG) grants SFB621-A1, SFB738-B5, SFB587-B3,
SFB900-B1 and KFO 250-FO 334/2-1. We regret that, owing to space limitations, we
could not always quote the work of colleagues who have contributed to the field.
author:
- first_name: Jean
full_name: Girard, Jean
last_name: Girard
- first_name: Christine
full_name: Moussion, Christine
id: 3356F664-F248-11E8-B48F-1D18A9856A87
last_name: Moussion
- first_name: Reinhold
full_name: Förster, Reinhold
last_name: Förster
citation:
ama: Girard J, Moussion C, Förster R. HEVs, lymphatics and homeostatic immune cell
trafficking in lymph nodes. Nature Reviews Immunology. 2012;12(11):762-773.
doi:10.1038/nri3298
apa: Girard, J., Moussion, C., & Förster, R. (2012). HEVs, lymphatics and homeostatic
immune cell trafficking in lymph nodes. Nature Reviews Immunology. Nature
Publishing Group. https://doi.org/10.1038/nri3298
chicago: Girard, Jean, Christine Moussion, and Reinhold Förster. “HEVs, Lymphatics
and Homeostatic Immune Cell Trafficking in Lymph Nodes.” Nature Reviews Immunology.
Nature Publishing Group, 2012. https://doi.org/10.1038/nri3298.
ieee: J. Girard, C. Moussion, and R. Förster, “HEVs, lymphatics and homeostatic
immune cell trafficking in lymph nodes,” Nature Reviews Immunology, vol.
12, no. 11. Nature Publishing Group, pp. 762–773, 2012.
ista: Girard J, Moussion C, Förster R. 2012. HEVs, lymphatics and homeostatic immune
cell trafficking in lymph nodes. Nature Reviews Immunology. 12(11), 762–773.
mla: Girard, Jean, et al. “HEVs, Lymphatics and Homeostatic Immune Cell Trafficking
in Lymph Nodes.” Nature Reviews Immunology, vol. 12, no. 11, Nature Publishing
Group, 2012, pp. 762–73, doi:10.1038/nri3298.
short: J. Girard, C. Moussion, R. Förster, Nature Reviews Immunology 12 (2012) 762–773.
date_created: 2018-12-11T12:00:29Z
date_published: 2012-11-01T00:00:00Z
date_updated: 2021-01-12T07:39:57Z
day: '01'
department:
- _id: MiSi
doi: 10.1038/nri3298
intvolume: ' 12'
issue: '11'
language:
- iso: eng
month: '11'
oa_version: None
page: 762 - 773
publication: Nature Reviews Immunology
publication_status: published
publisher: Nature Publishing Group
publist_id: '3787'
quality_controlled: '1'
scopus_import: 1
status: public
title: HEVs, lymphatics and homeostatic immune cell trafficking in lymph nodes
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 12
year: '2012'
...
---
_id: '2949'
author:
- first_name: David
full_name: Dupret, David
last_name: Dupret
- first_name: Jozsef L
full_name: Csicsvari, Jozsef L
id: 3FA14672-F248-11E8-B48F-1D18A9856A87
last_name: Csicsvari
orcid: 0000-0002-5193-4036
citation:
ama: Dupret D, Csicsvari JL. The medial entorhinal cortex keeps Up. Nature Neuroscience.
2012;15(11):1471-1472. doi:10.1038/nn.3245
apa: Dupret, D., & Csicsvari, J. L. (2012). The medial entorhinal cortex keeps
Up. Nature Neuroscience. Nature Publishing Group. https://doi.org/10.1038/nn.3245
chicago: Dupret, David, and Jozsef L Csicsvari. “The Medial Entorhinal Cortex Keeps
Up.” Nature Neuroscience. Nature Publishing Group, 2012. https://doi.org/10.1038/nn.3245.
ieee: D. Dupret and J. L. Csicsvari, “The medial entorhinal cortex keeps Up,” Nature
Neuroscience, vol. 15, no. 11. Nature Publishing Group, pp. 1471–1472, 2012.
ista: Dupret D, Csicsvari JL. 2012. The medial entorhinal cortex keeps Up. Nature
Neuroscience. 15(11), 1471–1472.
mla: Dupret, David, and Jozsef L. Csicsvari. “The Medial Entorhinal Cortex Keeps
Up.” Nature Neuroscience, vol. 15, no. 11, Nature Publishing Group, 2012,
pp. 1471–72, doi:10.1038/nn.3245.
short: D. Dupret, J.L. Csicsvari, Nature Neuroscience 15 (2012) 1471–1472.
date_created: 2018-12-11T12:00:30Z
date_published: 2012-11-01T00:00:00Z
date_updated: 2021-01-12T07:39:59Z
day: '01'
department:
- _id: JoCs
doi: 10.1038/nn.3245
intvolume: ' 15'
issue: '11'
language:
- iso: eng
main_file_link:
- url: http://www.mrcbndu.ox.ac.uk/publications/medial-entorhinal-cortex-keeps
month: '11'
oa_version: None
page: 1471 - 1472
publication: Nature Neuroscience
publication_status: published
publisher: Nature Publishing Group
publist_id: '3782'
quality_controlled: '1'
scopus_import: 1
status: public
title: The medial entorhinal cortex keeps Up
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 15
year: '2012'
...
---
_id: '2954'
abstract:
- lang: eng
text: Spontaneous postsynaptic currents (PSCs) provide key information about the
mechanisms of synaptic transmission and the activity modes of neuronal networks.
However, detecting spontaneous PSCs in vitro and in vivo has been challenging,
because of the small amplitude, the variable kinetics, and the undefined time
of generation of these events. Here, we describe a, to our knowledge, new method
for detecting spontaneous synaptic events by deconvolution, using a template that
approximates the average time course of spontaneous PSCs. A recorded PSC trace
is deconvolved from the template, resulting in a series of delta-like functions.
The maxima of these delta-like events are reliably detected, revealing the precise
onset times of the spontaneous PSCs. Among all detection methods, the deconvolution-based
method has a unique temporal resolution, allowing the detection of individual
events in high-frequency bursts. Furthermore, the deconvolution-based method has
a high amplitude resolution, because deconvolution can substantially increase
the signal/noise ratio. When tested against previously published methods using
experimental data, the deconvolution-based method was superior for spontaneous
PSCs recorded in vivo. Using the high-resolution deconvolution-based detection
algorithm, we show that the frequency of spontaneous excitatory postsynaptic currents
in dentate gyrus granule cells is 4.5 times higher in vivo than in vitro.
acknowledgement: "This work was supported by the Deutsche Forschungsgemeinschaft (TR3/B10)
and a European Research Council Advanced grant to P.J.\r\nWe thank H. Hu, S. J.
Guzman, and C. Schmidt-Hieber for critically reading the manuscript, I. Koeva and
F. Marr for technical support, and E. Kramberger for editorial assistance.\r\n"
author:
- first_name: Alejandro
full_name: Pernia-Andrade, Alejandro
id: 36963E98-F248-11E8-B48F-1D18A9856A87
last_name: Pernia-Andrade
- first_name: Sarit
full_name: Goswami, Sarit
id: 3A578F32-F248-11E8-B48F-1D18A9856A87
last_name: Goswami
- first_name: Yvonne
full_name: Stickler, Yvonne
id: 63B76600-E9CC-11E9-9B5F-82450873F7A1
last_name: Stickler
- first_name: Ulrich
full_name: Fröbe, Ulrich
last_name: Fröbe
- first_name: Alois
full_name: Schlögl, Alois
id: 45BF87EE-F248-11E8-B48F-1D18A9856A87
last_name: Schlögl
orcid: 0000-0002-5621-8100
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Pernia-Andrade A, Goswami S, Stickler Y, Fröbe U, Schlögl A, Jonas PM. A deconvolution
based method with high sensitivity and temporal resolution for detection of spontaneous
synaptic currents in vitro and in vivo. Biophysical Journal. 2012;103(7):1429-1439.
doi:10.1016/j.bpj.2012.08.039
apa: Pernia-Andrade, A., Goswami, S., Stickler, Y., Fröbe, U., Schlögl, A., &
Jonas, P. M. (2012). A deconvolution based method with high sensitivity and temporal
resolution for detection of spontaneous synaptic currents in vitro and in vivo.
Biophysical Journal. Biophysical. https://doi.org/10.1016/j.bpj.2012.08.039
chicago: Pernia-Andrade, Alejandro, Sarit Goswami, Yvonne Stickler, Ulrich Fröbe,
Alois Schlögl, and Peter M Jonas. “A Deconvolution Based Method with High Sensitivity
and Temporal Resolution for Detection of Spontaneous Synaptic Currents in Vitro
and in Vivo.” Biophysical Journal. Biophysical, 2012. https://doi.org/10.1016/j.bpj.2012.08.039.
ieee: A. Pernia-Andrade, S. Goswami, Y. Stickler, U. Fröbe, A. Schlögl, and P. M.
Jonas, “A deconvolution based method with high sensitivity and temporal resolution
for detection of spontaneous synaptic currents in vitro and in vivo,” Biophysical
Journal, vol. 103, no. 7. Biophysical, pp. 1429–1439, 2012.
ista: Pernia-Andrade A, Goswami S, Stickler Y, Fröbe U, Schlögl A, Jonas PM. 2012.
A deconvolution based method with high sensitivity and temporal resolution for
detection of spontaneous synaptic currents in vitro and in vivo. Biophysical Journal.
103(7), 1429–1439.
mla: Pernia-Andrade, Alejandro, et al. “A Deconvolution Based Method with High Sensitivity
and Temporal Resolution for Detection of Spontaneous Synaptic Currents in Vitro
and in Vivo.” Biophysical Journal, vol. 103, no. 7, Biophysical, 2012,
pp. 1429–39, doi:10.1016/j.bpj.2012.08.039.
short: A. Pernia-Andrade, S. Goswami, Y. Stickler, U. Fröbe, A. Schlögl, P.M. Jonas,
Biophysical Journal 103 (2012) 1429–1439.
date_created: 2018-12-11T12:00:32Z
date_published: 2012-10-03T00:00:00Z
date_updated: 2021-01-12T07:40:01Z
day: '03'
department:
- _id: PeJo
- _id: ScienComp
doi: 10.1016/j.bpj.2012.08.039
external_id:
pmid:
- '23062335'
intvolume: ' 103'
issue: '7'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3471482/
month: '10'
oa: 1
oa_version: Submitted Version
page: 1429 - 1439
pmid: 1
project:
- _id: 25BDE9A4-B435-11E9-9278-68D0E5697425
grant_number: SFB-TR3-TP10B
name: Glutamaterge synaptische Übertragung und Plastizität in hippocampalen Mikroschaltkreisen
publication: Biophysical Journal
publication_status: published
publisher: Biophysical
publist_id: '3774'
quality_controlled: '1'
scopus_import: 1
status: public
title: A deconvolution based method with high sensitivity and temporal resolution
for detection of spontaneous synaptic currents in vitro and in vivo
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 103
year: '2012'
...
---
_id: '2950'
abstract:
- lang: eng
text: Contractile actomyosin rings drive various fundamental morphogenetic processes
ranging from cytokinesis to wound healing. Actomyosin rings are generally thought
to function by circumferential contraction. Here, we show that the spreading of
the enveloping cell layer (EVL) over the yolk cell during zebrafish gastrulation
is driven by a contractile actomyosin ring. In contrast to previous suggestions,
we find that this ring functions not only by circumferential contraction but also
by a flow-friction mechanism. This generates a pulling force through resistance
against retrograde actomyosin flow. EVL spreading proceeds normally in situations
where circumferential contraction is unproductive, indicating that the flow-friction
mechanism is sufficient. Thus, actomyosin rings can function in epithelial morphogenesis
through a combination of cable-constriction and flow-friction mechanisms.
acknowledged_ssus:
- _id: SSU
author:
- first_name: Martin
full_name: Behrndt, Martin
id: 3ECECA3A-F248-11E8-B48F-1D18A9856A87
last_name: Behrndt
- first_name: Guillaume
full_name: Salbreux, Guillaume
last_name: Salbreux
- first_name: Pedro
full_name: Campinho, Pedro
id: 3AFBBC42-F248-11E8-B48F-1D18A9856A87
last_name: Campinho
orcid: 0000-0002-8526-5416
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Felix
full_name: Oswald, Felix
last_name: Oswald
- first_name: Julia
full_name: Roensch, Julia
id: 4220E59C-F248-11E8-B48F-1D18A9856A87
last_name: Roensch
- first_name: Stephan
full_name: Grill, Stephan
last_name: Grill
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Behrndt M, Salbreux G, Campinho P, et al. Forces driving epithelial spreading
in zebrafish gastrulation. Science. 2012;338(6104):257-260. doi:10.1126/science.1224143
apa: Behrndt, M., Salbreux, G., Campinho, P., Hauschild, R., Oswald, F., Roensch,
J., … Heisenberg, C.-P. J. (2012). Forces driving epithelial spreading in zebrafish
gastrulation. Science. American Association for the Advancement of Science.
https://doi.org/10.1126/science.1224143
chicago: Behrndt, Martin, Guillaume Salbreux, Pedro Campinho, Robert Hauschild,
Felix Oswald, Julia Roensch, Stephan Grill, and Carl-Philipp J Heisenberg. “Forces
Driving Epithelial Spreading in Zebrafish Gastrulation.” Science. American
Association for the Advancement of Science, 2012. https://doi.org/10.1126/science.1224143.
ieee: M. Behrndt et al., “Forces driving epithelial spreading in zebrafish
gastrulation,” Science, vol. 338, no. 6104. American Association for the
Advancement of Science, pp. 257–260, 2012.
ista: Behrndt M, Salbreux G, Campinho P, Hauschild R, Oswald F, Roensch J, Grill
S, Heisenberg C-PJ. 2012. Forces driving epithelial spreading in zebrafish gastrulation.
Science. 338(6104), 257–260.
mla: Behrndt, Martin, et al. “Forces Driving Epithelial Spreading in Zebrafish Gastrulation.”
Science, vol. 338, no. 6104, American Association for the Advancement of
Science, 2012, pp. 257–60, doi:10.1126/science.1224143.
short: M. Behrndt, G. Salbreux, P. Campinho, R. Hauschild, F. Oswald, J. Roensch,
S. Grill, C.-P.J. Heisenberg, Science 338 (2012) 257–260.
date_created: 2018-12-11T12:00:30Z
date_published: 2012-10-12T00:00:00Z
date_updated: 2023-02-21T17:02:44Z
day: '12'
department:
- _id: CaHe
- _id: Bio
doi: 10.1126/science.1224143
intvolume: ' 338'
issue: '6104'
language:
- iso: eng
month: '10'
oa_version: None
page: 257 - 260
project:
- _id: 252ABD0A-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I 930-B20
name: Control of Epithelial Cell Layer Spreading in Zebrafish
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '3778'
quality_controlled: '1'
related_material:
record:
- id: '1403'
relation: dissertation_contains
status: public
scopus_import: 1
status: public
title: Forces driving epithelial spreading in zebrafish gastrulation
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 338
year: '2012'
...
---
_id: '2951'
abstract:
- lang: eng
text: Differential cell adhesion and cortex tension are thought to drive cell sorting
by controlling cell-cell contact formation. Here, we show that cell adhesion and
cortex tension have different mechanical functions in controlling progenitor cell-cell
contact formation and sorting during zebrafish gastrulation. Cortex tension controls
cell-cell contact expansion by modulating interfacial tension at the contact.
By contrast, adhesion has little direct function in contact expansion, but instead
is needed to mechanically couple the cortices of adhering cells at their contacts,
allowing cortex tension to control contact expansion. The coupling function of
adhesion is mediated by E-cadherin and limited by the mechanical anchoring of
E-cadherin to the cortex. Thus, cell adhesion provides the mechanical scaffold
for cell cortex tension to drive cell sorting during gastrulation.
acknowledged_ssus:
- _id: SSU
author:
- first_name: Jean-Léon
full_name: Maître, Jean-Léon
id: 48F1E0D8-F248-11E8-B48F-1D18A9856A87
last_name: Maître
orcid: 0000-0002-3688-1474
- first_name: Hélène
full_name: Berthoumieux, Hélène
last_name: Berthoumieux
- first_name: Gabriel
full_name: Krens, Gabriel
id: 2B819732-F248-11E8-B48F-1D18A9856A87
last_name: Krens
orcid: 0000-0003-4761-5996
- first_name: Guillaume
full_name: Salbreux, Guillaume
last_name: Salbreux
- first_name: Frank
full_name: Julicher, Frank
last_name: Julicher
- first_name: Ewa
full_name: Paluch, Ewa
last_name: Paluch
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Maître J-L, Berthoumieux H, Krens G, et al. Adhesion functions in cell sorting
by mechanically coupling the cortices of adhering cells. Science. 2012;338(6104):253-256.
doi:10.1126/science.1225399
apa: Maître, J.-L., Berthoumieux, H., Krens, G., Salbreux, G., Julicher, F., Paluch,
E., & Heisenberg, C.-P. J. (2012). Adhesion functions in cell sorting by mechanically
coupling the cortices of adhering cells. Science. American Association
for the Advancement of Science. https://doi.org/10.1126/science.1225399
chicago: Maître, Jean-Léon, Hélène Berthoumieux, Gabriel Krens, Guillaume Salbreux,
Frank Julicher, Ewa Paluch, and Carl-Philipp J Heisenberg. “Adhesion Functions
in Cell Sorting by Mechanically Coupling the Cortices of Adhering Cells.” Science.
American Association for the Advancement of Science, 2012. https://doi.org/10.1126/science.1225399.
ieee: J.-L. Maître et al., “Adhesion functions in cell sorting by mechanically
coupling the cortices of adhering cells,” Science, vol. 338, no. 6104.
American Association for the Advancement of Science, pp. 253–256, 2012.
ista: Maître J-L, Berthoumieux H, Krens G, Salbreux G, Julicher F, Paluch E, Heisenberg
C-PJ. 2012. Adhesion functions in cell sorting by mechanically coupling the cortices
of adhering cells. Science. 338(6104), 253–256.
mla: Maître, Jean-Léon, et al. “Adhesion Functions in Cell Sorting by Mechanically
Coupling the Cortices of Adhering Cells.” Science, vol. 338, no. 6104,
American Association for the Advancement of Science, 2012, pp. 253–56, doi:10.1126/science.1225399.
short: J.-L. Maître, H. Berthoumieux, G. Krens, G. Salbreux, F. Julicher, E. Paluch,
C.-P.J. Heisenberg, Science 338 (2012) 253–256.
date_created: 2018-12-11T12:00:31Z
date_published: 2012-10-12T00:00:00Z
date_updated: 2021-01-12T07:40:00Z
day: '12'
department:
- _id: CaHe
doi: 10.1126/science.1225399
intvolume: ' 338'
issue: '6104'
language:
- iso: eng
month: '10'
oa_version: None
page: 253 - 256
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '3777'
quality_controlled: '1'
scopus_import: 1
status: public
title: Adhesion functions in cell sorting by mechanically coupling the cortices of
adhering cells
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 338
year: '2012'
...