--- _id: '10387' abstract: - lang: eng text: We report numerical simulations of membrane tubulation driven by large colloidal particles. Using Monte Carlo simulations we study how the process depends on particle size and binding strength, and present accurate free energy calculations to sort out how tube formation compares with the competing budding process. We find that tube formation is a result of the collective behavior of the particles adhering on the surface, and it occurs for binding strengths that are smaller than those required for budding. We also find that long linear aggregates of particles forming on the membrane surface act as nucleation seeds for tubulation by lowering the free energy barrier associated to the process. article_number: '188101' article_processing_charge: No article_type: original author: - first_name: Anđela full_name: Šarić, Anđela id: bf63d406-f056-11eb-b41d-f263a6566d8b last_name: Šarić orcid: 0000-0002-7854-2139 - first_name: Angelo full_name: Cacciuto, Angelo last_name: Cacciuto citation: ama: Šarić A, Cacciuto A. Mechanism of membrane tube formation induced by adhesive nanocomponents. Physical Review Letters. 2012;109(18). doi:10.1103/physrevlett.109.188101 apa: Šarić, A., & Cacciuto, A. (2012). Mechanism of membrane tube formation induced by adhesive nanocomponents. Physical Review Letters. American Physical Society. https://doi.org/10.1103/physrevlett.109.188101 chicago: Šarić, Anđela, and Angelo Cacciuto. “Mechanism of Membrane Tube Formation Induced by Adhesive Nanocomponents.” Physical Review Letters. American Physical Society, 2012. https://doi.org/10.1103/physrevlett.109.188101. ieee: A. Šarić and A. Cacciuto, “Mechanism of membrane tube formation induced by adhesive nanocomponents,” Physical Review Letters, vol. 109, no. 18. American Physical Society, 2012. ista: Šarić A, Cacciuto A. 2012. Mechanism of membrane tube formation induced by adhesive nanocomponents. Physical Review Letters. 109(18), 188101. mla: Šarić, Anđela, and Angelo Cacciuto. “Mechanism of Membrane Tube Formation Induced by Adhesive Nanocomponents.” Physical Review Letters, vol. 109, no. 18, 188101, American Physical Society, 2012, doi:10.1103/physrevlett.109.188101. short: A. Šarić, A. Cacciuto, Physical Review Letters 109 (2012). date_created: 2021-11-29T14:08:00Z date_published: 2012-10-31T00:00:00Z date_updated: 2021-11-29T14:29:25Z day: '31' doi: 10.1103/physrevlett.109.188101 extern: '1' external_id: arxiv: - '1206.3528' pmid: - '23215334' intvolume: ' 109' issue: '18' keyword: - general physics and astronomy language: - iso: eng main_file_link: - open_access: '1' url: https://arxiv.org/abs/1206.3528 month: '10' oa: 1 oa_version: Preprint pmid: 1 publication: Physical Review Letters publication_identifier: eissn: - 1079-7114 issn: - 0031-9007 publication_status: published publisher: American Physical Society quality_controlled: '1' scopus_import: '1' status: public title: Mechanism of membrane tube formation induced by adhesive nanocomponents type: journal_article user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9 volume: 109 year: '2012' ... --- _id: '10388' abstract: - lang: eng text: Using computer simulations, we show that lipid membranes can mediate linear aggregation of spherical nanoparticles binding to it for a wide range of biologically relevant bending rigidities. This result is in net contrast with the isotropic aggregation of nanoparticles on fluid interfaces or the expected clustering of isotropic insertions in biological membranes. We present a phase diagram indicating where linear aggregation is expected and compute explicitly the free-energy barriers associated with linear and isotropic aggregation. Finally, we provide simple scaling arguments to explain this phenomenology. acknowledgement: "This work was supported by the National Science Foundation under Career Grant No. DMR-0846426.\r\n" article_number: '118101' article_processing_charge: No article_type: original author: - first_name: Anđela full_name: Šarić, Anđela id: bf63d406-f056-11eb-b41d-f263a6566d8b last_name: Šarić orcid: 0000-0002-7854-2139 - first_name: Angelo full_name: Cacciuto, Angelo last_name: Cacciuto citation: ama: Šarić A, Cacciuto A. Fluid membranes can drive linear aggregation of adsorbed spherical nanoparticles. Physical Review Letters. 2012;108(11). doi:10.1103/physrevlett.108.118101 apa: Šarić, A., & Cacciuto, A. (2012). Fluid membranes can drive linear aggregation of adsorbed spherical nanoparticles. Physical Review Letters. American Physical Society. https://doi.org/10.1103/physrevlett.108.118101 chicago: Šarić, Anđela, and Angelo Cacciuto. “Fluid Membranes Can Drive Linear Aggregation of Adsorbed Spherical Nanoparticles.” Physical Review Letters. American Physical Society, 2012. https://doi.org/10.1103/physrevlett.108.118101. ieee: A. Šarić and A. Cacciuto, “Fluid membranes can drive linear aggregation of adsorbed spherical nanoparticles,” Physical Review Letters, vol. 108, no. 11. American Physical Society, 2012. ista: Šarić A, Cacciuto A. 2012. Fluid membranes can drive linear aggregation of adsorbed spherical nanoparticles. Physical Review Letters. 108(11), 118101. mla: Šarić, Anđela, and Angelo Cacciuto. “Fluid Membranes Can Drive Linear Aggregation of Adsorbed Spherical Nanoparticles.” Physical Review Letters, vol. 108, no. 11, 118101, American Physical Society, 2012, doi:10.1103/physrevlett.108.118101. short: A. Šarić, A. Cacciuto, Physical Review Letters 108 (2012). date_created: 2021-11-29T14:30:05Z date_published: 2012-03-14T00:00:00Z date_updated: 2021-11-29T15:12:13Z day: '14' doi: 10.1103/physrevlett.108.118101 extern: '1' external_id: arxiv: - '1201.0036' pmid: - '22540513' intvolume: ' 108' issue: '11' keyword: - general physics and astronomy language: - iso: eng main_file_link: - open_access: '1' url: https://arxiv.org/abs/1201.0036 month: '03' oa: 1 oa_version: Preprint pmid: 1 publication: Physical Review Letters publication_identifier: eissn: - 1079-7114 issn: - 0031-9007 publication_status: published publisher: American Physical Society quality_controlled: '1' scopus_import: '1' status: public title: Fluid membranes can drive linear aggregation of adsorbed spherical nanoparticles type: journal_article user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9 volume: 108 year: '2012' ... --- _id: '1055' abstract: - lang: eng text: In July, 2011, a 32-year-old man presented with thoracic pain radiating to the left arm and upper dorsum, shortness of breath, and palpitations. He had had upper back tension for 6 months. Medical history was unremarkable apart from moderate nicotine use (two pack-years). Echocardiography, electrocardiography, and laboratory tests were unremarkable, excluding a cardiac event. CT of the chest after chest radiography showed a large bulla of 16 cm diameter in the right hemithorax (figure A). We did not detect radiological evidence of underlying pulmonary disease. The bulla wall was unremarkable and no structures were seen within the bulla. acknowledgement: We thank the interdisciplinary team at the ELK Berlin Chest Hospital. article_processing_charge: No author: - first_name: Barbara full_name: Erne, Barbara last_name: Erne - first_name: Mareike full_name: Graff, Mareike last_name: Graff - first_name: Wolfram full_name: Klemm, Wolfram last_name: Klemm - first_name: Johann G full_name: Danzl, Johann G id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87 last_name: Danzl orcid: 0000-0001-8559-3973 - first_name: Gunda full_name: Leschber, Gunda last_name: Leschber citation: ama: Erne B, Graff M, Klemm W, Danzl JG, Leschber G. Bulla in the lung. The Lancet. 2012;380(9849). doi:10.1016/S0140-6736(12)60690-4 apa: Erne, B., Graff, M., Klemm, W., Danzl, J. G., & Leschber, G. (2012). Bulla in the lung. The Lancet. Elsevier. https://doi.org/10.1016/S0140-6736(12)60690-4 chicago: Erne, Barbara, Mareike Graff, Wolfram Klemm, Johann G Danzl, and Gunda Leschber. “Bulla in the Lung.” The Lancet. Elsevier, 2012. https://doi.org/10.1016/S0140-6736(12)60690-4. ieee: B. Erne, M. Graff, W. Klemm, J. G. Danzl, and G. Leschber, “Bulla in the lung,” The Lancet, vol. 380, no. 9849. Elsevier, 2012. ista: Erne B, Graff M, Klemm W, Danzl JG, Leschber G. 2012. Bulla in the lung. The Lancet. 380(9849). mla: Erne, Barbara, et al. “Bulla in the Lung.” The Lancet, vol. 380, no. 9849, Elsevier, 2012, doi:10.1016/S0140-6736(12)60690-4. short: B. Erne, M. Graff, W. Klemm, J.G. Danzl, G. Leschber, The Lancet 380 (2012). date_created: 2018-12-11T11:49:54Z date_published: 2012-10-01T00:00:00Z date_updated: 2021-01-12T06:47:57Z day: '01' doi: 10.1016/S0140-6736(12)60690-4 extern: '1' intvolume: ' 380' issue: '9849' language: - iso: eng month: '10' oa_version: None publication: The Lancet publication_status: published publisher: Elsevier publist_id: '6333' status: public title: Bulla in the lung type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 380 year: '2012' ... --- _id: '1056' abstract: - lang: eng text: We prepare and study a metastable attractive Mott-insulator state formed with bosonic atoms in a three-dimensional optical lattice. Starting from a Mott insulator with Cs atoms at weak repulsive interactions, we use a magnetic Feshbach resonance to tune the interactions to large attractive values and produce a metastable state pinned by attractive interactions with a lifetime on the order of 10 s. We probe the (de)excitation spectrum via lattice modulation spectroscopy, measuring the interaction dependence of two- and three-body bound-state energies. As a result of increased on-site three-body loss we observe resonance broadening and suppression of tunneling processes that produce three-body occupation. acknowledgement: We are indebted to R. Grimm for generous support. We thank J. von Stecher, P. Johnson, and E. Tiesinga for fruitful discussions. We gratefully acknowledge funding by the Austrian Science Fund (FWF) within Project No. I153-N16 and within the framework of the European Science Foundation (ESF) EuroQUASAR collective research project QuDeGPM, and by the European Research Council (ERC) under Project No. 278417. article_processing_charge: No author: - first_name: Manfred full_name: Mark, Manfred last_name: Mark - first_name: Elmar full_name: Haller, Elmar last_name: Haller - first_name: Katharina full_name: Lauber, Katharina last_name: Lauber - first_name: Johann G full_name: Danzl, Johann G id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87 last_name: Danzl orcid: 0000-0001-8559-3973 - first_name: Alexander full_name: Janisch, Alexander last_name: Janisch - first_name: Hans full_name: Büchler, Hans last_name: Büchler - first_name: Andrew full_name: Daley, Andrew last_name: Daley - first_name: Hanns full_name: Nägerl, Hanns last_name: Nägerl citation: ama: Mark M, Haller E, Lauber K, et al. Preparation and spectroscopy of a metastable mott-insulator state with attractive interactions. Physical Review Letters. 2012;108(21). doi:10.1103/PhysRevLett.108.215302 apa: Mark, M., Haller, E., Lauber, K., Danzl, J. G., Janisch, A., Büchler, H., … Nägerl, H. (2012). Preparation and spectroscopy of a metastable mott-insulator state with attractive interactions. Physical Review Letters. American Physical Society. https://doi.org/10.1103/PhysRevLett.108.215302 chicago: Mark, Manfred, Elmar Haller, Katharina Lauber, Johann G Danzl, Alexander Janisch, Hans Büchler, Andrew Daley, and Hanns Nägerl. “Preparation and Spectroscopy of a Metastable Mott-Insulator State with Attractive Interactions.” Physical Review Letters. American Physical Society, 2012. https://doi.org/10.1103/PhysRevLett.108.215302. ieee: M. Mark et al., “Preparation and spectroscopy of a metastable mott-insulator state with attractive interactions,” Physical Review Letters, vol. 108, no. 21. American Physical Society, 2012. ista: Mark M, Haller E, Lauber K, Danzl JG, Janisch A, Büchler H, Daley A, Nägerl H. 2012. Preparation and spectroscopy of a metastable mott-insulator state with attractive interactions. Physical Review Letters. 108(21). mla: Mark, Manfred, et al. “Preparation and Spectroscopy of a Metastable Mott-Insulator State with Attractive Interactions.” Physical Review Letters, vol. 108, no. 21, American Physical Society, 2012, doi:10.1103/PhysRevLett.108.215302. short: M. Mark, E. Haller, K. Lauber, J.G. Danzl, A. Janisch, H. Büchler, A. Daley, H. Nägerl, Physical Review Letters 108 (2012). date_created: 2018-12-11T11:49:55Z date_published: 2012-05-25T00:00:00Z date_updated: 2021-01-12T06:47:58Z day: '25' doi: 10.1103/PhysRevLett.108.215302 extern: '1' external_id: arxiv: - '1201.1008' intvolume: ' 108' issue: '21' language: - iso: eng main_file_link: - open_access: '1' url: https://arxiv.org/abs/1201.1008 month: '05' oa: 1 oa_version: Preprint publication: Physical Review Letters publication_status: published publisher: American Physical Society publist_id: '6334' status: public title: Preparation and spectroscopy of a metastable mott-insulator state with attractive interactions type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 108 year: '2012' ... --- _id: '10750' abstract: - lang: eng text: The goal of this work is to study the superconducting coherence length in the fluctuation regime in cuprate superconductors. In this work we present cantilever torque magnetometry measurements of micron-size BSCCO flakes patterned with arrays of nanometer scale rings or holes. Using ultrasensitive dynamic torque magnetometry, oscillations in magnetization are observed near Tc as a function of the applied magnetic flux threading the array. Special effort was made to detect the oscillations in magnetization at temperatures above Tc, where the Nernst effect and magnetization measurements suggest the possibility of pairing. To constrain the magnitude of the coherence length in the fluctuation regime, we will present the dependence of the amplitude of the h/2e period oscillations as a function of temperature and hole size. acknowledgement: This work was supported by the Center for Emergent Superconductivity, an Energy Frontier Research Center funded by the U.S. DOE, Office of Science, under Award No. DE-AC0298CH1088. alternative_title: - Bulletin of the American Physical Society article_number: X21.00008 article_processing_charge: No author: - first_name: Hryhoriy full_name: Polshyn, Hryhoriy id: edfc7cb1-526e-11ec-b05a-e6ecc27e4e48 last_name: Polshyn orcid: 0000-0001-8223-8896 - first_name: Raffi full_name: Budakian, Raffi last_name: Budakian citation: ama: 'Polshyn H, Budakian R. Cantilever torque magnetometry study of multiply connected BSCCO arrays near Tc. In: APS March Meeting 2012. Vol 57. American Physical Society; 2012.' apa: 'Polshyn, H., & Budakian, R. (2012). Cantilever torque magnetometry study of multiply connected BSCCO arrays near Tc. In APS March Meeting 2012 (Vol. 57). Boston, MA, United States: American Physical Society.' chicago: Polshyn, Hryhoriy, and Raffi Budakian. “Cantilever Torque Magnetometry Study of Multiply Connected BSCCO Arrays near Tc.” In APS March Meeting 2012, Vol. 57. American Physical Society, 2012. ieee: H. Polshyn and R. Budakian, “Cantilever torque magnetometry study of multiply connected BSCCO arrays near Tc,” in APS March Meeting 2012, Boston, MA, United States, 2012, vol. 57, no. 1. ista: 'Polshyn H, Budakian R. 2012. Cantilever torque magnetometry study of multiply connected BSCCO arrays near Tc. APS March Meeting 2012. APS: American Physical Society, Bulletin of the American Physical Society, vol. 57, X21.00008.' mla: Polshyn, Hryhoriy, and Raffi Budakian. “Cantilever Torque Magnetometry Study of Multiply Connected BSCCO Arrays near Tc.” APS March Meeting 2012, vol. 57, no. 1, X21.00008, American Physical Society, 2012. short: H. Polshyn, R. Budakian, in:, APS March Meeting 2012, American Physical Society, 2012. conference: end_date: 2012-03-02 location: Boston, MA, United States name: 'APS: American Physical Society' start_date: 2012-02-27 date_created: 2022-02-08T10:39:08Z date_published: 2012-02-01T00:00:00Z date_updated: 2022-02-08T10:48:01Z day: '01' extern: '1' intvolume: ' 57' issue: '1' language: - iso: eng main_file_link: - open_access: '1' url: https://meetings.aps.org/Meeting/MAR12/Event/167014 month: '02' oa: 1 oa_version: Published Version publication: APS March Meeting 2012 publication_identifier: issn: - 0003-0503 publication_status: published publisher: American Physical Society quality_controlled: '1' status: public title: Cantilever torque magnetometry study of multiply connected BSCCO arrays near Tc type: conference user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9 volume: 57 year: '2012' ... --- _id: '10896' abstract: - lang: eng text: Under physiological conditions the brain, via the purine salvage pathway, reuses the preformed purine bases hypoxanthine, derived from ATP degradation, and adenine (Ade), derived from polyamine synthesis, to restore its ATP pool. However, the massive degradation of ATP during ischemia, although providing valuable neuroprotective adenosine, results in the accumulation and loss of diffusible purine metabolites and thereby leads to a protracted reduction in the post-ischemic ATP pool size. In vivo, this may both limit the ability to deploy ATP-dependent reparative mechanisms and reduce the subsequent availability of adenosine, whilst in brain slices results in tissue with substantially lower levels of ATP than in vivo. In the present review, we describe the mechanisms by which brain tissue replenishes its ATP, how this can be improved with the clinically tolerated chemicals D-ribose and adenine, and the functional, and potential therapeutic, implications of doing so. acknowledgement: We are grateful to Research into Ageing/Ageing UK and The Dunhill Trust for funding SzN’s graduate studies, and to Prof Nicholas Dale for his valuable input. article_processing_charge: No author: - first_name: Stephanie full_name: zur Nedden, Stephanie id: 3C77F464-F248-11E8-B48F-1D18A9856A87 last_name: zur Nedden - first_name: Alexander S. full_name: Doney, Alexander S. last_name: Doney - first_name: Bruno G. full_name: Frenguelli, Bruno G. last_name: Frenguelli citation: ama: 'zur Nedden S, Doney AS, Frenguelli BG. The double-edged sword: Gaining Adenosine at the expense of ATP. How to balance the books. In: Masino S, Boison D, eds. Adenosine. 1st ed. New York: Springer; 2012:109-129. doi:10.1007/978-1-4614-3903-5_6' apa: 'zur Nedden, S., Doney, A. S., & Frenguelli, B. G. (2012). The double-edged sword: Gaining Adenosine at the expense of ATP. How to balance the books. In S. Masino & D. Boison (Eds.), Adenosine (1st ed., pp. 109–129). New York: Springer. https://doi.org/10.1007/978-1-4614-3903-5_6' chicago: 'Nedden, Stephanie zur, Alexander S. Doney, and Bruno G. Frenguelli. “The Double-Edged Sword: Gaining Adenosine at the Expense of ATP. How to Balance the Books.” In Adenosine, edited by Susan Masino and Detlev Boison, 1st ed., 109–29. New York: Springer, 2012. https://doi.org/10.1007/978-1-4614-3903-5_6.' ieee: 'S. zur Nedden, A. S. Doney, and B. G. Frenguelli, “The double-edged sword: Gaining Adenosine at the expense of ATP. How to balance the books,” in Adenosine, 1st ed., S. Masino and D. Boison, Eds. New York: Springer, 2012, pp. 109–129.' ista: 'zur Nedden S, Doney AS, Frenguelli BG. 2012.The double-edged sword: Gaining Adenosine at the expense of ATP. How to balance the books. In: Adenosine. , 109–129.' mla: 'zur Nedden, Stephanie, et al. “The Double-Edged Sword: Gaining Adenosine at the Expense of ATP. How to Balance the Books.” Adenosine, edited by Susan Masino and Detlev Boison, 1st ed., Springer, 2012, pp. 109–29, doi:10.1007/978-1-4614-3903-5_6.' short: S. zur Nedden, A.S. Doney, B.G. Frenguelli, in:, S. Masino, D. Boison (Eds.), Adenosine, 1st ed., Springer, New York, 2012, pp. 109–129. date_created: 2022-03-21T07:16:12Z date_published: 2012-07-23T00:00:00Z date_updated: 2022-06-21T11:51:58Z day: '23' department: - _id: HaJa doi: 10.1007/978-1-4614-3903-5_6 edition: '1' editor: - first_name: Susan full_name: Masino, Susan last_name: Masino - first_name: Detlev full_name: Boison, Detlev last_name: Boison language: - iso: eng month: '07' oa_version: None page: 109-129 place: New York publication: Adenosine publication_identifier: eisbn: - '9781461439035' isbn: - '9781461439028' publication_status: published publisher: Springer quality_controlled: '1' scopus_import: '1' status: public title: 'The double-edged sword: Gaining Adenosine at the expense of ATP. How to balance the books' type: book_chapter user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 year: '2012' ... --- _id: '11089' abstract: - lang: eng text: The Nuclear Envelope (NE) contains over 100 different proteins that associate with nuclear components such as chromatin, the lamina and the transcription machinery. Mutations in genes encoding NE proteins have been shown to result in tissue-specific defects and disease, suggesting cell-type specific differences in NE composition and function. Consistent with these observations, recent studies have revealed unexpected functions for numerous NE associated proteins during cell differentiation and development. Here we review the latest insights into the roles played by the NE in cell differentiation, development, disease and aging, focusing primarily on inner nuclear membrane (INM) proteins and nuclear pore components. article_processing_charge: No article_type: original author: - first_name: J Sebastian full_name: Gomez-Cavazos, J Sebastian last_name: Gomez-Cavazos - first_name: Martin W full_name: HETZER, Martin W id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed last_name: HETZER orcid: 0000-0002-2111-992X citation: ama: 'Gomez-Cavazos JS, Hetzer M. Outfits for different occasions: tissue-specific roles of Nuclear Envelope proteins. Current Opinion in Cell Biology. 2012;24(6):775-783. doi:10.1016/j.ceb.2012.08.008' apa: 'Gomez-Cavazos, J. S., & Hetzer, M. (2012). Outfits for different occasions: tissue-specific roles of Nuclear Envelope proteins. Current Opinion in Cell Biology. Elsevier. https://doi.org/10.1016/j.ceb.2012.08.008' chicago: 'Gomez-Cavazos, J Sebastian, and Martin Hetzer. “Outfits for Different Occasions: Tissue-Specific Roles of Nuclear Envelope Proteins.” Current Opinion in Cell Biology. Elsevier, 2012. https://doi.org/10.1016/j.ceb.2012.08.008.' ieee: 'J. S. Gomez-Cavazos and M. Hetzer, “Outfits for different occasions: tissue-specific roles of Nuclear Envelope proteins,” Current Opinion in Cell Biology, vol. 24, no. 6. Elsevier, pp. 775–783, 2012.' ista: 'Gomez-Cavazos JS, Hetzer M. 2012. Outfits for different occasions: tissue-specific roles of Nuclear Envelope proteins. Current Opinion in Cell Biology. 24(6), 775–783.' mla: 'Gomez-Cavazos, J. Sebastian, and Martin Hetzer. “Outfits for Different Occasions: Tissue-Specific Roles of Nuclear Envelope Proteins.” Current Opinion in Cell Biology, vol. 24, no. 6, Elsevier, 2012, pp. 775–83, doi:10.1016/j.ceb.2012.08.008.' short: J.S. Gomez-Cavazos, M. Hetzer, Current Opinion in Cell Biology 24 (2012) 775–783. date_created: 2022-04-07T07:51:37Z date_published: 2012-12-01T00:00:00Z date_updated: 2022-07-18T08:38:47Z day: '01' doi: 10.1016/j.ceb.2012.08.008 extern: '1' external_id: pmid: - '22995343' intvolume: ' 24' issue: '6' keyword: - Cell Biology language: - iso: eng month: '12' oa_version: None page: 775-783 pmid: 1 publication: Current Opinion in Cell Biology publication_identifier: issn: - 0955-0674 publication_status: published publisher: Elsevier quality_controlled: '1' scopus_import: '1' status: public title: 'Outfits for different occasions: tissue-specific roles of Nuclear Envelope proteins' type: journal_article user_id: 72615eeb-f1f3-11ec-aa25-d4573ddc34fd volume: 24 year: '2012' ... --- _id: '11091' abstract: - lang: eng text: Neoplastic cells are often characterized by specific morphological abnormalities of the nuclear envelope (NE), which have been used for cancer diagnosis for more than a century. The NE is a double phospholipid bilayer that encapsulates the nuclear genome, regulates all nuclear trafficking of RNAs and proteins and prevents the passive diffusion of macromolecules between the nucleoplasm and the cytoplasm. Whether there is a consequence to the proper functioning of the cell and loss of structural integrity of the nucleus remains unclear. Using live cell imaging, we characterize a phenomenon wherein nuclei of several proliferating human cancer cell lines become temporarily ruptured during interphase. Strikingly, NE rupturing was associated with the mislocalization of nucleoplasmic and cytoplasmic proteins and, in the most extreme cases, the entrapment of cytoplasmic organelles in the nuclear interior. In addition, we observed the formation of micronuclei-like structures during interphase and the movement of chromatin out of the nuclear space. The frequency of these NE rupturing events was higher in cells in which the nuclear lamina, a network of intermediate filaments providing mechanical support to the NE, was not properly formed. Our data uncover the existence of a NE instability that has the potential to change the genomic landscape of cancer cells. article_processing_charge: No article_type: original author: - first_name: Jesse D. full_name: Vargas, Jesse D. last_name: Vargas - first_name: Emily M. full_name: Hatch, Emily M. last_name: Hatch - first_name: Daniel J. full_name: Anderson, Daniel J. last_name: Anderson - first_name: Martin W full_name: HETZER, Martin W id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed last_name: HETZER orcid: 0000-0002-2111-992X citation: ama: Vargas JD, Hatch EM, Anderson DJ, Hetzer M. Transient nuclear envelope rupturing during interphase in human cancer cells. Nucleus. 2012;3(1):88-100. doi:10.4161/nucl.18954 apa: Vargas, J. D., Hatch, E. M., Anderson, D. J., & Hetzer, M. (2012). Transient nuclear envelope rupturing during interphase in human cancer cells. Nucleus. Taylor & Francis. https://doi.org/10.4161/nucl.18954 chicago: Vargas, Jesse D., Emily M. Hatch, Daniel J. Anderson, and Martin Hetzer. “Transient Nuclear Envelope Rupturing during Interphase in Human Cancer Cells.” Nucleus. Taylor & Francis, 2012. https://doi.org/10.4161/nucl.18954. ieee: J. D. Vargas, E. M. Hatch, D. J. Anderson, and M. Hetzer, “Transient nuclear envelope rupturing during interphase in human cancer cells,” Nucleus, vol. 3, no. 1. Taylor & Francis, pp. 88–100, 2012. ista: Vargas JD, Hatch EM, Anderson DJ, Hetzer M. 2012. Transient nuclear envelope rupturing during interphase in human cancer cells. Nucleus. 3(1), 88–100. mla: Vargas, Jesse D., et al. “Transient Nuclear Envelope Rupturing during Interphase in Human Cancer Cells.” Nucleus, vol. 3, no. 1, Taylor & Francis, 2012, pp. 88–100, doi:10.4161/nucl.18954. short: J.D. Vargas, E.M. Hatch, D.J. Anderson, M. Hetzer, Nucleus 3 (2012) 88–100. date_created: 2022-04-07T07:51:53Z date_published: 2012-01-01T00:00:00Z date_updated: 2022-07-18T08:52:53Z day: '01' doi: 10.4161/nucl.18954 extern: '1' external_id: pmid: - '22567193' intvolume: ' 3' issue: '1' keyword: - Cell Biology language: - iso: eng month: '01' oa_version: None page: 88-100 pmid: 1 publication: Nucleus publication_identifier: eissn: - 1949-1042 issn: - 1949-1034 publication_status: published publisher: Taylor & Francis quality_controlled: '1' scopus_import: '1' status: public title: Transient nuclear envelope rupturing during interphase in human cancer cells type: journal_article user_id: 72615eeb-f1f3-11ec-aa25-d4573ddc34fd volume: 3 year: '2012' ... --- _id: '11093' abstract: - lang: eng text: Nuclear pore complexes (NPCs) are built from ∼30 different proteins called nucleoporins or Nups. Previous studies have shown that several Nups exhibit cell-type-specific expression and that mutations in NPC components result in tissue-specific diseases. Here we show that a specific change in NPC composition is required for both myogenic and neuronal differentiation. The transmembrane nucleoporin Nup210 is absent in proliferating myoblasts and embryonic stem cells (ESCs) but becomes expressed and incorporated into NPCs during cell differentiation. Preventing Nup210 production by RNAi blocks myogenesis and the differentiation of ESCs into neuroprogenitors. We found that the addition of Nup210 to NPCs does not affect nuclear transport but is required for the induction of genes that are essential for cell differentiation. Our results identify a single change in NPC composition as an essential step in cell differentiation and establish a role for Nup210 in gene expression regulation and cell fate determination. article_processing_charge: No article_type: original author: - first_name: Maximiliano A. full_name: D'Angelo, Maximiliano A. last_name: D'Angelo - first_name: J. Sebastian full_name: Gomez-Cavazos, J. Sebastian last_name: Gomez-Cavazos - first_name: Arianna full_name: Mei, Arianna last_name: Mei - first_name: Daniel H. full_name: Lackner, Daniel H. last_name: Lackner - first_name: Martin W full_name: HETZER, Martin W id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed last_name: HETZER orcid: 0000-0002-2111-992X citation: ama: D’Angelo MA, Gomez-Cavazos JS, Mei A, Lackner DH, Hetzer M. A change in nuclear pore complex composition regulates cell differentiation. Developmental Cell. 2012;22(2):446-458. doi:10.1016/j.devcel.2011.11.021 apa: D’Angelo, M. A., Gomez-Cavazos, J. S., Mei, A., Lackner, D. H., & Hetzer, M. (2012). A change in nuclear pore complex composition regulates cell differentiation. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2011.11.021 chicago: D’Angelo, Maximiliano A., J. Sebastian Gomez-Cavazos, Arianna Mei, Daniel H. Lackner, and Martin Hetzer. “A Change in Nuclear Pore Complex Composition Regulates Cell Differentiation.” Developmental Cell. Elsevier, 2012. https://doi.org/10.1016/j.devcel.2011.11.021. ieee: M. A. D’Angelo, J. S. Gomez-Cavazos, A. Mei, D. H. Lackner, and M. Hetzer, “A change in nuclear pore complex composition regulates cell differentiation,” Developmental Cell, vol. 22, no. 2. Elsevier, pp. 446–458, 2012. ista: D’Angelo MA, Gomez-Cavazos JS, Mei A, Lackner DH, Hetzer M. 2012. A change in nuclear pore complex composition regulates cell differentiation. Developmental Cell. 22(2), 446–458. mla: D’Angelo, Maximiliano A., et al. “A Change in Nuclear Pore Complex Composition Regulates Cell Differentiation.” Developmental Cell, vol. 22, no. 2, Elsevier, 2012, pp. 446–58, doi:10.1016/j.devcel.2011.11.021. short: M.A. D’Angelo, J.S. Gomez-Cavazos, A. Mei, D.H. Lackner, M. Hetzer, Developmental Cell 22 (2012) 446–458. date_created: 2022-04-07T07:52:10Z date_published: 2012-01-19T00:00:00Z date_updated: 2022-07-18T08:53:16Z day: '19' doi: 10.1016/j.devcel.2011.11.021 extern: '1' external_id: pmid: - '22264802' intvolume: ' 22' issue: '2' keyword: - Developmental Biology - Cell Biology - General Biochemistry - Genetics and Molecular Biology - Molecular Biology language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.1016/j.devcel.2011.11.021 month: '01' oa: 1 oa_version: Published Version page: 446-458 pmid: 1 publication: Developmental Cell publication_identifier: issn: - 1534-5807 publication_status: published publisher: Elsevier quality_controlled: '1' scopus_import: '1' status: public title: A change in nuclear pore complex composition regulates cell differentiation type: journal_article user_id: 72615eeb-f1f3-11ec-aa25-d4573ddc34fd volume: 22 year: '2012' ... --- _id: '11092' abstract: - lang: eng text: To combat the functional decline of the proteome, cells use the process of protein turnover to replace potentially impaired polypeptides with new functional copies. We found that extremely long-lived proteins (ELLPs) did not turn over in postmitotic cells of the rat central nervous system. These ELLPs were associated with chromatin and the nuclear pore complex, the central transport channels that mediate all molecular trafficking in and out of the nucleus. The longevity of these proteins would be expected to expose them to potentially harmful metabolites, putting them at risk of accumulating damage over extended periods of time. Thus, it is possible that failure to maintain proper levels and functional integrity of ELLPs in nonproliferative cells might contribute to age-related deterioration in cell and tissue function. article_processing_charge: No article_type: letter_note author: - first_name: Jeffrey N. full_name: Savas, Jeffrey N. last_name: Savas - first_name: Brandon H. full_name: Toyama, Brandon H. last_name: Toyama - first_name: Tao full_name: Xu, Tao last_name: Xu - first_name: John R. full_name: Yates, John R. last_name: Yates - first_name: Martin W full_name: HETZER, Martin W id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed last_name: HETZER orcid: 0000-0002-2111-992X citation: ama: Savas JN, Toyama BH, Xu T, Yates JR, Hetzer M. Extremely long-lived nuclear pore proteins in the rat brain. Science. 2012;335(6071):942-942. doi:10.1126/science.1217421 apa: Savas, J. N., Toyama, B. H., Xu, T., Yates, J. R., & Hetzer, M. (2012). Extremely long-lived nuclear pore proteins in the rat brain. Science. American Association for the Advancement of Science. https://doi.org/10.1126/science.1217421 chicago: Savas, Jeffrey N., Brandon H. Toyama, Tao Xu, John R. Yates, and Martin Hetzer. “Extremely Long-Lived Nuclear Pore Proteins in the Rat Brain.” Science. American Association for the Advancement of Science, 2012. https://doi.org/10.1126/science.1217421. ieee: J. N. Savas, B. H. Toyama, T. Xu, J. R. Yates, and M. Hetzer, “Extremely long-lived nuclear pore proteins in the rat brain,” Science, vol. 335, no. 6071. American Association for the Advancement of Science, pp. 942–942, 2012. ista: Savas JN, Toyama BH, Xu T, Yates JR, Hetzer M. 2012. Extremely long-lived nuclear pore proteins in the rat brain. Science. 335(6071), 942–942. mla: Savas, Jeffrey N., et al. “Extremely Long-Lived Nuclear Pore Proteins in the Rat Brain.” Science, vol. 335, no. 6071, American Association for the Advancement of Science, 2012, pp. 942–942, doi:10.1126/science.1217421. short: J.N. Savas, B.H. Toyama, T. Xu, J.R. Yates, M. Hetzer, Science 335 (2012) 942–942. date_created: 2022-04-07T07:52:01Z date_published: 2012-02-02T00:00:00Z date_updated: 2022-07-18T08:53:06Z day: '02' doi: 10.1126/science.1217421 extern: '1' external_id: pmid: - '22300851' intvolume: ' 335' issue: '6071' keyword: - Multidisciplinary language: - iso: eng month: '02' oa_version: None page: 942-942 pmid: 1 publication: Science publication_identifier: eissn: - 1095-9203 issn: - 0036-8075 publication_status: published publisher: American Association for the Advancement of Science quality_controlled: '1' scopus_import: '1' status: public title: Extremely long-lived nuclear pore proteins in the rat brain type: journal_article user_id: 72615eeb-f1f3-11ec-aa25-d4573ddc34fd volume: 335 year: '2012' ...