--- _id: '3012' abstract: - lang: eng text: Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport-related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux. author: - first_name: Jan full_name: Petrášek, Jan last_name: Petrášek - first_name: Jozef full_name: Mravec, Jozef last_name: Mravec - first_name: Rodolphe full_name: Bouchard, Rodolphe last_name: Bouchard - first_name: Joshua full_name: Blakeslee, Joshua last_name: Blakeslee - first_name: Melinda F full_name: Melinda Abas id: 3CFB3B1C-F248-11E8-B48F-1D18A9856A87 last_name: Abas - first_name: Daniela full_name: Seifertová, Daniela last_name: Seifertová - first_name: Justyna full_name: Wiśniewska, Justyna last_name: Wiśniewska - first_name: Zerihun full_name: Tadele, Zerihun last_name: Tadele - first_name: Martin full_name: Kubeš, Martin last_name: Kubeš - first_name: Milada full_name: Čovanová, Milada last_name: Čovanová - first_name: Pankaj full_name: Dhonukshe, Pankaj last_name: Dhonukshe - first_name: Petr full_name: Skůpa, Petr last_name: Skůpa - first_name: Eva full_name: Eva Benková id: 38F4F166-F248-11E8-B48F-1D18A9856A87 last_name: Benková orcid: 0000-0002-8510-9739 - first_name: Lucie full_name: Perry, Lucie last_name: Perry - first_name: Pavel full_name: Křeček, Pavel last_name: Křeček - first_name: Ok full_name: Lee, Ok Ran last_name: Lee - first_name: Gerald full_name: Fink, Gerald R last_name: Fink - first_name: Markus full_name: Geisler, Markus last_name: Geisler - first_name: Angus full_name: Murphy, Angus S last_name: Murphy - first_name: Christian full_name: Luschnig, Christian last_name: Luschnig - first_name: Eva full_name: Zažímalová, Eva last_name: Zažímalová - first_name: Jirí full_name: Jirí Friml id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 citation: ama: Petrášek J, Mravec J, Bouchard R, et al. PIN proteins perform a rate-limiting function in cellular auxin efflux. Science. 2006;312(5775):914-918. doi:10.1126/science.1123542 apa: Petrášek, J., Mravec, J., Bouchard, R., Blakeslee, J., Abas, M. F., Seifertová, D., … Friml, J. (2006). PIN proteins perform a rate-limiting function in cellular auxin efflux. Science. American Association for the Advancement of Science. https://doi.org/10.1126/science.1123542 chicago: Petrášek, Jan, Jozef Mravec, Rodolphe Bouchard, Joshua Blakeslee, Melinda F Abas, Daniela Seifertová, Justyna Wiśniewska, et al. “PIN Proteins Perform a Rate-Limiting Function in Cellular Auxin Efflux.” Science. American Association for the Advancement of Science, 2006. https://doi.org/10.1126/science.1123542. ieee: J. Petrášek et al., “PIN proteins perform a rate-limiting function in cellular auxin efflux,” Science, vol. 312, no. 5775. American Association for the Advancement of Science, pp. 914–918, 2006. ista: Petrášek J, Mravec J, Bouchard R, Blakeslee J, Abas MF, Seifertová D, Wiśniewska J, Tadele Z, Kubeš M, Čovanová M, Dhonukshe P, Skůpa P, Benková E, Perry L, Křeček P, Lee O, Fink G, Geisler M, Murphy A, Luschnig C, Zažímalová E, Friml J. 2006. PIN proteins perform a rate-limiting function in cellular auxin efflux. Science. 312(5775), 914–918. mla: Petrášek, Jan, et al. “PIN Proteins Perform a Rate-Limiting Function in Cellular Auxin Efflux.” Science, vol. 312, no. 5775, American Association for the Advancement of Science, 2006, pp. 914–18, doi:10.1126/science.1123542. short: J. Petrášek, J. Mravec, R. Bouchard, J. Blakeslee, M.F. Abas, D. Seifertová, J. Wiśniewska, Z. Tadele, M. Kubeš, M. Čovanová, P. Dhonukshe, P. Skůpa, E. Benková, L. Perry, P. Křeček, O. Lee, G. Fink, M. Geisler, A. Murphy, C. Luschnig, E. Zažímalová, J. Friml, Science 312 (2006) 914–918. date_created: 2018-12-11T12:00:51Z date_published: 2006-05-12T00:00:00Z date_updated: 2021-01-12T07:40:27Z day: '12' doi: 10.1126/science.1123542 extern: 1 intvolume: ' 312' issue: '5775' month: '05' page: 914 - 918 publication: Science publication_status: published publisher: American Association for the Advancement of Science publist_id: '3690' quality_controlled: 0 status: public title: PIN proteins perform a rate-limiting function in cellular auxin efflux type: journal_article volume: 312 year: '2006' ... --- _id: '3010' abstract: - lang: eng text: The formation of the leaf vascular pattern has fascinated biologists for centuries. In the early leaf primordium, complex networks of procambial cells emerge from homogeneous subepidermal tissue. The molecular nature of the underlying positional information is unknown, but various lines of evidence implicate gradually restricted transport routes of the plant hormone auxin in defining sites of procambium formation. Here we show that a crucial member of the AtPIN family of auxin-efflux-associated proteins, AtPIN1, is expressed prior to pre-procambial and procambial cell fate markers in domains that become restricted toward sites of procambium formation. Subcellular AtPIN1 polarity indicates that auxin is directed to distinct "convergence points" in the epidermis, from where it defines the positions of major veins. Integrated polarities in all emerging veins indicate auxin drainage toward pre-existing veins, but veins display divergent polarities as they become connected at both ends. Auxin application and transport inhibition reveal that convergence point positioning and AtPIN1 expression domain dynamics are self-organizing, auxin-transport-dependent processes. We derive a model for self-regulated, reiterative patterning of all vein orders and postulate at its onset a common epidermal auxin-focusing mechanism for major-vein positioning and phyllotactic patterning. author: - first_name: Enrico full_name: Scarpella, Enrico last_name: Scarpella - first_name: Danielle full_name: Marcos, Danielle last_name: Marcos - first_name: Jirí full_name: Jirí Friml id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 - first_name: Thomas full_name: Berleth, Thomas last_name: Berleth citation: ama: Scarpella E, Marcos D, Friml J, Berleth T. Control of leaf vascular patterning by polar auxin transport. Genes and Development. 2006;20(8):1015-1027. doi:10.1101/gad.1402406 apa: Scarpella, E., Marcos, D., Friml, J., & Berleth, T. (2006). Control of leaf vascular patterning by polar auxin transport. Genes and Development. Cold Spring Harbor Laboratory Press. https://doi.org/10.1101/gad.1402406 chicago: Scarpella, Enrico, Danielle Marcos, Jiří Friml, and Thomas Berleth. “Control of Leaf Vascular Patterning by Polar Auxin Transport.” Genes and Development. Cold Spring Harbor Laboratory Press, 2006. https://doi.org/10.1101/gad.1402406. ieee: E. Scarpella, D. Marcos, J. Friml, and T. Berleth, “Control of leaf vascular patterning by polar auxin transport,” Genes and Development, vol. 20, no. 8. Cold Spring Harbor Laboratory Press, pp. 1015–1027, 2006. ista: Scarpella E, Marcos D, Friml J, Berleth T. 2006. Control of leaf vascular patterning by polar auxin transport. Genes and Development. 20(8), 1015–1027. mla: Scarpella, Enrico, et al. “Control of Leaf Vascular Patterning by Polar Auxin Transport.” Genes and Development, vol. 20, no. 8, Cold Spring Harbor Laboratory Press, 2006, pp. 1015–27, doi:10.1101/gad.1402406. short: E. Scarpella, D. Marcos, J. Friml, T. Berleth, Genes and Development 20 (2006) 1015–1027. date_created: 2018-12-11T12:00:51Z date_published: 2006-04-15T00:00:00Z date_updated: 2021-01-12T07:40:26Z day: '15' doi: 10.1101/gad.1402406 extern: 1 intvolume: ' 20' issue: '8' month: '04' page: 1015 - 1027 publication: Genes and Development publication_status: published publisher: Cold Spring Harbor Laboratory Press publist_id: '3692' quality_controlled: 0 status: public title: Control of leaf vascular patterning by polar auxin transport type: journal_article volume: 20 year: '2006' ... --- _id: '3007' abstract: - lang: eng text: Root gravitropism describes the orientation of root growth along the gravity vector and is mediated by differential cell elongation in the root meristem. This response requires the coordinated, asymmetric distribution of the phytohormone auxin within the root meristem, and depends on the concerted activities of PIN proteins and AUX1 - members of the auxin transport pathway. Here, we show that intracellular trafficking and proteasome activity combine to control PIN2 degradation during root gravitropism. Following gravi-stimulation, proteasome-dependent variations in PIN2 localization and degradation at the upper and lower sides of the root result in asymmetric distribution of PIN2. Ubiquitination of PIN2 occurs in a proteasome-dependent manner, indicating that the proteasome is involved in the control of PIN2 turnover. Stabilization of PIN2 affects its abundance and distribution, and leads to defects in auxin distribution and gravitropic responses. We describe the effects of auxin on PIN2 localization and protein levels, indicating that redistribution of auxin during the gravitropic response may be involved in the regulation of PIN2 protein. author: - first_name: Lindy full_name: Abas, Lindy last_name: Abas - first_name: René full_name: Benjamins, René last_name: Benjamins - first_name: Nenad full_name: Malenica, Nenad last_name: Malenica - first_name: Tomasz full_name: Paciorek, Tomasz last_name: Paciorek - first_name: Justyna full_name: Wiśniewska, Justyna last_name: Wiśniewska - first_name: Jeanette full_name: Moulinier-Anzola, Jeanette C last_name: Moulinier Anzola - first_name: Tobias full_name: Sieberer, Tobias last_name: Sieberer - first_name: Jirí full_name: Jirí Friml id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 - first_name: Christian full_name: Luschnig, Christian last_name: Luschnig citation: ama: Abas L, Benjamins R, Malenica N, et al. Intracellular trafficking and proteolysis of the Arabidopsis auxin-efflux facilitator PIN2 are involved in root gravitropism. Nature Cell Biology. 2006;8(3):249-256. doi:10.1038/ncb1369 apa: Abas, L., Benjamins, R., Malenica, N., Paciorek, T., Wiśniewska, J., Moulinier Anzola, J., … Luschnig, C. (2006). Intracellular trafficking and proteolysis of the Arabidopsis auxin-efflux facilitator PIN2 are involved in root gravitropism. Nature Cell Biology. Nature Publishing Group. https://doi.org/10.1038/ncb1369 chicago: Abas, Lindy, René Benjamins, Nenad Malenica, Tomasz Paciorek, Justyna Wiśniewska, Jeanette Moulinier Anzola, Tobias Sieberer, Jiří Friml, and Christian Luschnig. “Intracellular Trafficking and Proteolysis of the Arabidopsis Auxin-Efflux Facilitator PIN2 Are Involved in Root Gravitropism.” Nature Cell Biology. Nature Publishing Group, 2006. https://doi.org/10.1038/ncb1369. ieee: L. Abas et al., “Intracellular trafficking and proteolysis of the Arabidopsis auxin-efflux facilitator PIN2 are involved in root gravitropism,” Nature Cell Biology, vol. 8, no. 3. Nature Publishing Group, pp. 249–256, 2006. ista: Abas L, Benjamins R, Malenica N, Paciorek T, Wiśniewska J, Moulinier Anzola J, Sieberer T, Friml J, Luschnig C. 2006. Intracellular trafficking and proteolysis of the Arabidopsis auxin-efflux facilitator PIN2 are involved in root gravitropism. Nature Cell Biology. 8(3), 249–256. mla: Abas, Lindy, et al. “Intracellular Trafficking and Proteolysis of the Arabidopsis Auxin-Efflux Facilitator PIN2 Are Involved in Root Gravitropism.” Nature Cell Biology, vol. 8, no. 3, Nature Publishing Group, 2006, pp. 249–56, doi:10.1038/ncb1369. short: L. Abas, R. Benjamins, N. Malenica, T. Paciorek, J. Wiśniewska, J. Moulinier Anzola, T. Sieberer, J. Friml, C. Luschnig, Nature Cell Biology 8 (2006) 249–256. date_created: 2018-12-11T12:00:50Z date_published: 2006-03-01T00:00:00Z date_updated: 2021-01-12T07:40:25Z day: '01' doi: 10.1038/ncb1369 extern: 1 intvolume: ' 8' issue: '3' month: '03' page: 249 - 256 publication: Nature Cell Biology publication_status: published publisher: Nature Publishing Group publist_id: '3694' quality_controlled: 0 status: public title: Intracellular trafficking and proteolysis of the Arabidopsis auxin-efflux facilitator PIN2 are involved in root gravitropism type: journal_article volume: 8 year: '2006' ... --- _id: '3006' abstract: - lang: eng text: 'Dividing plant cells perform a remarkable task of building a new cell wall within the cytoplasm in a few minutes. A long-standing paradigm claims that this primordial cell wall, known as the cell plate, is generated by delivery of newly synthesized material from Golgi apparatus-originated secretory vesicles. Here, we show that, in diverse plant species, cell surface material, including plasma membrane proteins, cell wall components, and exogenously applied endocytic tracers, is rapidly delivered to the forming cell plate. Importantly, this occurs even when de novo protein synthesis is blocked. In addition, cytokinesis-specific syntaxin KNOLLE as well as plasma membrane (PM) resident proteins localize to endosomes that fuse to initiate the cell plate. The rate of endocytosis is strongly enhanced during cell plate formation, and its genetic or pharmacological inhibition leads to cytokinesis defects. Our results reveal that endocytic delivery of cell surface material significantly contributes to cell plate formation during plant cytokinesis. ' author: - first_name: Pankaj full_name: Dhonukshe, Pankaj last_name: Dhonukshe - first_name: František full_name: Baluška, František last_name: Baluška - first_name: Markus full_name: Schlicht, Markus last_name: Schlicht - first_name: Andrej full_name: Hlavacka, Andrej last_name: Hlavacka - first_name: Jozef full_name: Šamaj, Jozef last_name: Šamaj - first_name: Jirí full_name: Jirí Friml id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 - first_name: Theodorus full_name: Gadella, Theodorus W last_name: Gadella citation: ama: Dhonukshe P, Baluška F, Schlicht M, et al. Endocytosis of cell surface material mediates cell plate formation during plant cytokinesis. Developmental Cell. 2006;10(1):137-150. doi:10.1016/j.devcel.2005.11.015 apa: Dhonukshe, P., Baluška, F., Schlicht, M., Hlavacka, A., Šamaj, J., Friml, J., & Gadella, T. (2006). Endocytosis of cell surface material mediates cell plate formation during plant cytokinesis. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2005.11.015 chicago: Dhonukshe, Pankaj, František Baluška, Markus Schlicht, Andrej Hlavacka, Jozef Šamaj, Jiří Friml, and Theodorus Gadella. “Endocytosis of Cell Surface Material Mediates Cell Plate Formation during Plant Cytokinesis.” Developmental Cell. Cell Press, 2006. https://doi.org/10.1016/j.devcel.2005.11.015. ieee: P. Dhonukshe et al., “Endocytosis of cell surface material mediates cell plate formation during plant cytokinesis,” Developmental Cell, vol. 10, no. 1. Cell Press, pp. 137–150, 2006. ista: Dhonukshe P, Baluška F, Schlicht M, Hlavacka A, Šamaj J, Friml J, Gadella T. 2006. Endocytosis of cell surface material mediates cell plate formation during plant cytokinesis. Developmental Cell. 10(1), 137–150. mla: Dhonukshe, Pankaj, et al. “Endocytosis of Cell Surface Material Mediates Cell Plate Formation during Plant Cytokinesis.” Developmental Cell, vol. 10, no. 1, Cell Press, 2006, pp. 137–50, doi:10.1016/j.devcel.2005.11.015. short: P. Dhonukshe, F. Baluška, M. Schlicht, A. Hlavacka, J. Šamaj, J. Friml, T. Gadella, Developmental Cell 10 (2006) 137–150. date_created: 2018-12-11T12:00:49Z date_published: 2006-01-01T00:00:00Z date_updated: 2021-01-12T07:40:24Z day: '01' doi: 10.1016/j.devcel.2005.11.015 extern: 1 intvolume: ' 10' issue: '1' month: '01' page: 137 - 150 publication: Developmental Cell publication_status: published publisher: Cell Press publist_id: '3696' quality_controlled: 0 status: public title: Endocytosis of cell surface material mediates cell plate formation during plant cytokinesis type: journal_article volume: 10 year: '2006' ... --- _id: '3011' abstract: - lang: eng text: Polar flow of the phytohormone auxin requires plasma membrane‐associated PIN proteins and underlies multiple developmental processes in plants. Here we address the importance of the polarity of subcellular PIN localization for the directionality of auxin transport in Arabidopsis thaliana. Expression of different PINs in the root epidermis revealed the importance of PIN polar positions for directional auxin flow and root gravitropic growth. Interfering with sequence-embedded polarity signals directly demonstrates that PIN polarity is a primary factor in determining the direction of auxin flow in meristematic tissues. This finding provides a crucial piece in the puzzle of how auxin flow can be redirected via rapid changes in PIN polarity. author: - first_name: Justyna full_name: Wiśniewska, Justyna last_name: Wiśniewska - first_name: Jian full_name: Xu, Jian last_name: Xu - first_name: Daniela full_name: Seifertová, Daniela last_name: Seifertová - first_name: Philip full_name: Brewer, Philip B last_name: Brewer - first_name: Kamil full_name: Růžička, Kamil last_name: Růžička - first_name: Ikram full_name: Blilou, Ikram last_name: Blilou - first_name: David full_name: Rouquié, David last_name: Rouquié - first_name: Eva full_name: Eva Benková id: 38F4F166-F248-11E8-B48F-1D18A9856A87 last_name: Benková orcid: 0000-0002-8510-9739 - first_name: Ben full_name: Scheres, Ben last_name: Scheres - first_name: Jirí full_name: Jirí Friml id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 citation: ama: Wiśniewska J, Xu J, Seifertová D, et al. Polar PIN localization directs auxin flow in plants. Science. 2006;312(5775). doi:10.1126/science.1121356 apa: Wiśniewska, J., Xu, J., Seifertová, D., Brewer, P., Růžička, K., Blilou, I., … Friml, J. (2006). Polar PIN localization directs auxin flow in plants. Science. American Association for the Advancement of Science. https://doi.org/10.1126/science.1121356 chicago: Wiśniewska, Justyna, Jian Xu, Daniela Seifertová, Philip Brewer, Kamil Růžička, Ikram Blilou, David Rouquié, Eva Benková, Ben Scheres, and Jiří Friml. “Polar PIN Localization Directs Auxin Flow in Plants.” Science. American Association for the Advancement of Science, 2006. https://doi.org/10.1126/science.1121356. ieee: J. Wiśniewska et al., “Polar PIN localization directs auxin flow in plants,” Science, vol. 312, no. 5775. American Association for the Advancement of Science, 2006. ista: Wiśniewska J, Xu J, Seifertová D, Brewer P, Růžička K, Blilou I, Rouquié D, Benková E, Scheres B, Friml J. 2006. Polar PIN localization directs auxin flow in plants. Science. 312(5775). mla: Wiśniewska, Justyna, et al. “Polar PIN Localization Directs Auxin Flow in Plants.” Science, vol. 312, no. 5775, American Association for the Advancement of Science, 2006, doi:10.1126/science.1121356. short: J. Wiśniewska, J. Xu, D. Seifertová, P. Brewer, K. Růžička, I. Blilou, D. Rouquié, E. Benková, B. Scheres, J. Friml, Science 312 (2006). date_created: 2018-12-11T12:00:51Z date_published: 2006-05-12T00:00:00Z date_updated: 2021-01-12T07:40:27Z day: '12' doi: 10.1126/science.1121356 extern: 1 intvolume: ' 312' issue: '5775' month: '05' publication: Science publication_status: published publisher: American Association for the Advancement of Science publist_id: '3691' quality_controlled: 0 status: public title: Polar PIN localization directs auxin flow in plants type: journal_article volume: 312 year: '2006' ...