---
_id: '3012'
abstract:
- lang: eng
text: Intercellular flow of the phytohormone auxin underpins multiple developmental
processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein
(PGP) transporters are crucial factors in auxin transport-related development,
yet the molecular function of PINs remains unknown. Here, we show that PINs mediate
auxin efflux from mammalian and yeast cells without needing additional plant-specific
factors. Conditional gain-of-function alleles and quantitative measurements of
auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the
action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to
auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement
of PINs in catalyzing cellular auxin efflux.
author:
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Jozef
full_name: Mravec, Jozef
last_name: Mravec
- first_name: Rodolphe
full_name: Bouchard, Rodolphe
last_name: Bouchard
- first_name: Joshua
full_name: Blakeslee, Joshua
last_name: Blakeslee
- first_name: Melinda F
full_name: Melinda Abas
id: 3CFB3B1C-F248-11E8-B48F-1D18A9856A87
last_name: Abas
- first_name: Daniela
full_name: Seifertová, Daniela
last_name: Seifertová
- first_name: Justyna
full_name: Wiśniewska, Justyna
last_name: Wiśniewska
- first_name: Zerihun
full_name: Tadele, Zerihun
last_name: Tadele
- first_name: Martin
full_name: Kubeš, Martin
last_name: Kubeš
- first_name: Milada
full_name: Čovanová, Milada
last_name: Čovanová
- first_name: Pankaj
full_name: Dhonukshe, Pankaj
last_name: Dhonukshe
- first_name: Petr
full_name: Skůpa, Petr
last_name: Skůpa
- first_name: Eva
full_name: Eva Benková
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Lucie
full_name: Perry, Lucie
last_name: Perry
- first_name: Pavel
full_name: Křeček, Pavel
last_name: Křeček
- first_name: Ok
full_name: Lee, Ok Ran
last_name: Lee
- first_name: Gerald
full_name: Fink, Gerald R
last_name: Fink
- first_name: Markus
full_name: Geisler, Markus
last_name: Geisler
- first_name: Angus
full_name: Murphy, Angus S
last_name: Murphy
- first_name: Christian
full_name: Luschnig, Christian
last_name: Luschnig
- first_name: Eva
full_name: Zažímalová, Eva
last_name: Zažímalová
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Petrášek J, Mravec J, Bouchard R, et al. PIN proteins perform a rate-limiting
function in cellular auxin efflux. Science. 2006;312(5775):914-918. doi:10.1126/science.1123542
apa: Petrášek, J., Mravec, J., Bouchard, R., Blakeslee, J., Abas, M. F., Seifertová,
D., … Friml, J. (2006). PIN proteins perform a rate-limiting function in cellular
auxin efflux. Science. American Association for the Advancement of Science.
https://doi.org/10.1126/science.1123542
chicago: Petrášek, Jan, Jozef Mravec, Rodolphe Bouchard, Joshua Blakeslee, Melinda
F Abas, Daniela Seifertová, Justyna Wiśniewska, et al. “PIN Proteins Perform a
Rate-Limiting Function in Cellular Auxin Efflux.” Science. American Association
for the Advancement of Science, 2006. https://doi.org/10.1126/science.1123542.
ieee: J. Petrášek et al., “PIN proteins perform a rate-limiting function
in cellular auxin efflux,” Science, vol. 312, no. 5775. American Association
for the Advancement of Science, pp. 914–918, 2006.
ista: Petrášek J, Mravec J, Bouchard R, Blakeslee J, Abas MF, Seifertová D, Wiśniewska
J, Tadele Z, Kubeš M, Čovanová M, Dhonukshe P, Skůpa P, Benková E, Perry L, Křeček
P, Lee O, Fink G, Geisler M, Murphy A, Luschnig C, Zažímalová E, Friml J. 2006.
PIN proteins perform a rate-limiting function in cellular auxin efflux. Science.
312(5775), 914–918.
mla: Petrášek, Jan, et al. “PIN Proteins Perform a Rate-Limiting Function in Cellular
Auxin Efflux.” Science, vol. 312, no. 5775, American Association for the
Advancement of Science, 2006, pp. 914–18, doi:10.1126/science.1123542.
short: J. Petrášek, J. Mravec, R. Bouchard, J. Blakeslee, M.F. Abas, D. Seifertová,
J. Wiśniewska, Z. Tadele, M. Kubeš, M. Čovanová, P. Dhonukshe, P. Skůpa, E. Benková,
L. Perry, P. Křeček, O. Lee, G. Fink, M. Geisler, A. Murphy, C. Luschnig, E. Zažímalová,
J. Friml, Science 312 (2006) 914–918.
date_created: 2018-12-11T12:00:51Z
date_published: 2006-05-12T00:00:00Z
date_updated: 2021-01-12T07:40:27Z
day: '12'
doi: 10.1126/science.1123542
extern: 1
intvolume: ' 312'
issue: '5775'
month: '05'
page: 914 - 918
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '3690'
quality_controlled: 0
status: public
title: PIN proteins perform a rate-limiting function in cellular auxin efflux
type: journal_article
volume: 312
year: '2006'
...
---
_id: '3010'
abstract:
- lang: eng
text: The formation of the leaf vascular pattern has fascinated biologists for centuries.
In the early leaf primordium, complex networks of procambial cells emerge from
homogeneous subepidermal tissue. The molecular nature of the underlying positional
information is unknown, but various lines of evidence implicate gradually restricted
transport routes of the plant hormone auxin in defining sites of procambium formation.
Here we show that a crucial member of the AtPIN family of auxin-efflux-associated
proteins, AtPIN1, is expressed prior to pre-procambial and procambial cell fate
markers in domains that become restricted toward sites of procambium formation.
Subcellular AtPIN1 polarity indicates that auxin is directed to distinct "convergence
points" in the epidermis, from where it defines the positions of major veins.
Integrated polarities in all emerging veins indicate auxin drainage toward pre-existing
veins, but veins display divergent polarities as they become connected at both
ends. Auxin application and transport inhibition reveal that convergence point
positioning and AtPIN1 expression domain dynamics are self-organizing, auxin-transport-dependent
processes. We derive a model for self-regulated, reiterative patterning of all
vein orders and postulate at its onset a common epidermal auxin-focusing mechanism
for major-vein positioning and phyllotactic patterning.
author:
- first_name: Enrico
full_name: Scarpella, Enrico
last_name: Scarpella
- first_name: Danielle
full_name: Marcos, Danielle
last_name: Marcos
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Thomas
full_name: Berleth, Thomas
last_name: Berleth
citation:
ama: Scarpella E, Marcos D, Friml J, Berleth T. Control of leaf vascular patterning
by polar auxin transport. Genes and Development. 2006;20(8):1015-1027.
doi:10.1101/gad.1402406
apa: Scarpella, E., Marcos, D., Friml, J., & Berleth, T. (2006). Control of
leaf vascular patterning by polar auxin transport. Genes and Development.
Cold Spring Harbor Laboratory Press. https://doi.org/10.1101/gad.1402406
chicago: Scarpella, Enrico, Danielle Marcos, Jiří Friml, and Thomas Berleth. “Control
of Leaf Vascular Patterning by Polar Auxin Transport.” Genes and Development.
Cold Spring Harbor Laboratory Press, 2006. https://doi.org/10.1101/gad.1402406.
ieee: E. Scarpella, D. Marcos, J. Friml, and T. Berleth, “Control of leaf vascular
patterning by polar auxin transport,” Genes and Development, vol. 20, no.
8. Cold Spring Harbor Laboratory Press, pp. 1015–1027, 2006.
ista: Scarpella E, Marcos D, Friml J, Berleth T. 2006. Control of leaf vascular
patterning by polar auxin transport. Genes and Development. 20(8), 1015–1027.
mla: Scarpella, Enrico, et al. “Control of Leaf Vascular Patterning by Polar Auxin
Transport.” Genes and Development, vol. 20, no. 8, Cold Spring Harbor Laboratory
Press, 2006, pp. 1015–27, doi:10.1101/gad.1402406.
short: E. Scarpella, D. Marcos, J. Friml, T. Berleth, Genes and Development 20 (2006)
1015–1027.
date_created: 2018-12-11T12:00:51Z
date_published: 2006-04-15T00:00:00Z
date_updated: 2021-01-12T07:40:26Z
day: '15'
doi: 10.1101/gad.1402406
extern: 1
intvolume: ' 20'
issue: '8'
month: '04'
page: 1015 - 1027
publication: Genes and Development
publication_status: published
publisher: Cold Spring Harbor Laboratory Press
publist_id: '3692'
quality_controlled: 0
status: public
title: Control of leaf vascular patterning by polar auxin transport
type: journal_article
volume: 20
year: '2006'
...
---
_id: '3007'
abstract:
- lang: eng
text: Root gravitropism describes the orientation of root growth along the gravity
vector and is mediated by differential cell elongation in the root meristem. This
response requires the coordinated, asymmetric distribution of the phytohormone
auxin within the root meristem, and depends on the concerted activities of PIN
proteins and AUX1 - members of the auxin transport pathway. Here, we show that
intracellular trafficking and proteasome activity combine to control PIN2 degradation
during root gravitropism. Following gravi-stimulation, proteasome-dependent variations
in PIN2 localization and degradation at the upper and lower sides of the root
result in asymmetric distribution of PIN2. Ubiquitination of PIN2 occurs in a
proteasome-dependent manner, indicating that the proteasome is involved in the
control of PIN2 turnover. Stabilization of PIN2 affects its abundance and distribution,
and leads to defects in auxin distribution and gravitropic responses. We describe
the effects of auxin on PIN2 localization and protein levels, indicating that
redistribution of auxin during the gravitropic response may be involved in the
regulation of PIN2 protein.
author:
- first_name: Lindy
full_name: Abas, Lindy
last_name: Abas
- first_name: René
full_name: Benjamins, René
last_name: Benjamins
- first_name: Nenad
full_name: Malenica, Nenad
last_name: Malenica
- first_name: Tomasz
full_name: Paciorek, Tomasz
last_name: Paciorek
- first_name: Justyna
full_name: Wiśniewska, Justyna
last_name: Wiśniewska
- first_name: Jeanette
full_name: Moulinier-Anzola, Jeanette C
last_name: Moulinier Anzola
- first_name: Tobias
full_name: Sieberer, Tobias
last_name: Sieberer
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Christian
full_name: Luschnig, Christian
last_name: Luschnig
citation:
ama: Abas L, Benjamins R, Malenica N, et al. Intracellular trafficking and proteolysis
of the Arabidopsis auxin-efflux facilitator PIN2 are involved in root gravitropism.
Nature Cell Biology. 2006;8(3):249-256. doi:10.1038/ncb1369
apa: Abas, L., Benjamins, R., Malenica, N., Paciorek, T., Wiśniewska, J., Moulinier
Anzola, J., … Luschnig, C. (2006). Intracellular trafficking and proteolysis of
the Arabidopsis auxin-efflux facilitator PIN2 are involved in root gravitropism.
Nature Cell Biology. Nature Publishing Group. https://doi.org/10.1038/ncb1369
chicago: Abas, Lindy, René Benjamins, Nenad Malenica, Tomasz Paciorek, Justyna Wiśniewska,
Jeanette Moulinier Anzola, Tobias Sieberer, Jiří Friml, and Christian Luschnig.
“Intracellular Trafficking and Proteolysis of the Arabidopsis Auxin-Efflux Facilitator
PIN2 Are Involved in Root Gravitropism.” Nature Cell Biology. Nature Publishing
Group, 2006. https://doi.org/10.1038/ncb1369.
ieee: L. Abas et al., “Intracellular trafficking and proteolysis of the Arabidopsis
auxin-efflux facilitator PIN2 are involved in root gravitropism,” Nature Cell
Biology, vol. 8, no. 3. Nature Publishing Group, pp. 249–256, 2006.
ista: Abas L, Benjamins R, Malenica N, Paciorek T, Wiśniewska J, Moulinier Anzola
J, Sieberer T, Friml J, Luschnig C. 2006. Intracellular trafficking and proteolysis
of the Arabidopsis auxin-efflux facilitator PIN2 are involved in root gravitropism.
Nature Cell Biology. 8(3), 249–256.
mla: Abas, Lindy, et al. “Intracellular Trafficking and Proteolysis of the Arabidopsis
Auxin-Efflux Facilitator PIN2 Are Involved in Root Gravitropism.” Nature Cell
Biology, vol. 8, no. 3, Nature Publishing Group, 2006, pp. 249–56, doi:10.1038/ncb1369.
short: L. Abas, R. Benjamins, N. Malenica, T. Paciorek, J. Wiśniewska, J. Moulinier
Anzola, T. Sieberer, J. Friml, C. Luschnig, Nature Cell Biology 8 (2006) 249–256.
date_created: 2018-12-11T12:00:50Z
date_published: 2006-03-01T00:00:00Z
date_updated: 2021-01-12T07:40:25Z
day: '01'
doi: 10.1038/ncb1369
extern: 1
intvolume: ' 8'
issue: '3'
month: '03'
page: 249 - 256
publication: Nature Cell Biology
publication_status: published
publisher: Nature Publishing Group
publist_id: '3694'
quality_controlled: 0
status: public
title: Intracellular trafficking and proteolysis of the Arabidopsis auxin-efflux facilitator
PIN2 are involved in root gravitropism
type: journal_article
volume: 8
year: '2006'
...
---
_id: '3006'
abstract:
- lang: eng
text: 'Dividing plant cells perform a remarkable task of building a new cell wall
within the cytoplasm in a few minutes. A long-standing paradigm claims that this
primordial cell wall, known as the cell plate, is generated by delivery of newly
synthesized material from Golgi apparatus-originated secretory vesicles. Here,
we show that, in diverse plant species, cell surface material, including plasma
membrane proteins, cell wall components, and exogenously applied endocytic tracers,
is rapidly delivered to the forming cell plate. Importantly, this occurs even
when de novo protein synthesis is blocked. In addition, cytokinesis-specific syntaxin
KNOLLE as well as plasma membrane (PM) resident proteins localize to endosomes
that fuse to initiate the cell plate. The rate of endocytosis is strongly enhanced
during cell plate formation, and its genetic or pharmacological inhibition leads
to cytokinesis defects. Our results reveal that endocytic delivery of cell surface
material significantly contributes to cell plate formation during plant cytokinesis. '
author:
- first_name: Pankaj
full_name: Dhonukshe, Pankaj
last_name: Dhonukshe
- first_name: František
full_name: Baluška, František
last_name: Baluška
- first_name: Markus
full_name: Schlicht, Markus
last_name: Schlicht
- first_name: Andrej
full_name: Hlavacka, Andrej
last_name: Hlavacka
- first_name: Jozef
full_name: Šamaj, Jozef
last_name: Šamaj
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Theodorus
full_name: Gadella, Theodorus W
last_name: Gadella
citation:
ama: Dhonukshe P, Baluška F, Schlicht M, et al. Endocytosis of cell surface material
mediates cell plate formation during plant cytokinesis. Developmental Cell.
2006;10(1):137-150. doi:10.1016/j.devcel.2005.11.015
apa: Dhonukshe, P., Baluška, F., Schlicht, M., Hlavacka, A., Šamaj, J., Friml, J.,
& Gadella, T. (2006). Endocytosis of cell surface material mediates cell plate
formation during plant cytokinesis. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2005.11.015
chicago: Dhonukshe, Pankaj, František Baluška, Markus Schlicht, Andrej Hlavacka,
Jozef Šamaj, Jiří Friml, and Theodorus Gadella. “Endocytosis of Cell Surface Material
Mediates Cell Plate Formation during Plant Cytokinesis.” Developmental Cell.
Cell Press, 2006. https://doi.org/10.1016/j.devcel.2005.11.015.
ieee: P. Dhonukshe et al., “Endocytosis of cell surface material mediates
cell plate formation during plant cytokinesis,” Developmental Cell, vol.
10, no. 1. Cell Press, pp. 137–150, 2006.
ista: Dhonukshe P, Baluška F, Schlicht M, Hlavacka A, Šamaj J, Friml J, Gadella
T. 2006. Endocytosis of cell surface material mediates cell plate formation during
plant cytokinesis. Developmental Cell. 10(1), 137–150.
mla: Dhonukshe, Pankaj, et al. “Endocytosis of Cell Surface Material Mediates Cell
Plate Formation during Plant Cytokinesis.” Developmental Cell, vol. 10,
no. 1, Cell Press, 2006, pp. 137–50, doi:10.1016/j.devcel.2005.11.015.
short: P. Dhonukshe, F. Baluška, M. Schlicht, A. Hlavacka, J. Šamaj, J. Friml, T.
Gadella, Developmental Cell 10 (2006) 137–150.
date_created: 2018-12-11T12:00:49Z
date_published: 2006-01-01T00:00:00Z
date_updated: 2021-01-12T07:40:24Z
day: '01'
doi: 10.1016/j.devcel.2005.11.015
extern: 1
intvolume: ' 10'
issue: '1'
month: '01'
page: 137 - 150
publication: Developmental Cell
publication_status: published
publisher: Cell Press
publist_id: '3696'
quality_controlled: 0
status: public
title: Endocytosis of cell surface material mediates cell plate formation during plant
cytokinesis
type: journal_article
volume: 10
year: '2006'
...
---
_id: '3011'
abstract:
- lang: eng
text: Polar flow of the phytohormone auxin requires plasma membrane‐associated PIN
proteins and underlies multiple developmental processes in plants. Here we address
the importance of the polarity of subcellular PIN localization for the directionality
of auxin transport in Arabidopsis thaliana. Expression of different PINs in the
root epidermis revealed the importance of PIN polar positions for directional
auxin flow and root gravitropic growth. Interfering with sequence-embedded polarity
signals directly demonstrates that PIN polarity is a primary factor in determining
the direction of auxin flow in meristematic tissues. This finding provides a crucial
piece in the puzzle of how auxin flow can be redirected via rapid changes in PIN
polarity.
author:
- first_name: Justyna
full_name: Wiśniewska, Justyna
last_name: Wiśniewska
- first_name: Jian
full_name: Xu, Jian
last_name: Xu
- first_name: Daniela
full_name: Seifertová, Daniela
last_name: Seifertová
- first_name: Philip
full_name: Brewer, Philip B
last_name: Brewer
- first_name: Kamil
full_name: Růžička, Kamil
last_name: Růžička
- first_name: Ikram
full_name: Blilou, Ikram
last_name: Blilou
- first_name: David
full_name: Rouquié, David
last_name: Rouquié
- first_name: Eva
full_name: Eva Benková
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Ben
full_name: Scheres, Ben
last_name: Scheres
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Wiśniewska J, Xu J, Seifertová D, et al. Polar PIN localization directs auxin
flow in plants. Science. 2006;312(5775). doi:10.1126/science.1121356
apa: Wiśniewska, J., Xu, J., Seifertová, D., Brewer, P., Růžička, K., Blilou, I.,
… Friml, J. (2006). Polar PIN localization directs auxin flow in plants. Science.
American Association for the Advancement of Science. https://doi.org/10.1126/science.1121356
chicago: Wiśniewska, Justyna, Jian Xu, Daniela Seifertová, Philip Brewer, Kamil
Růžička, Ikram Blilou, David Rouquié, Eva Benková, Ben Scheres, and Jiří Friml.
“Polar PIN Localization Directs Auxin Flow in Plants.” Science. American
Association for the Advancement of Science, 2006. https://doi.org/10.1126/science.1121356.
ieee: J. Wiśniewska et al., “Polar PIN localization directs auxin flow in
plants,” Science, vol. 312, no. 5775. American Association for the Advancement
of Science, 2006.
ista: Wiśniewska J, Xu J, Seifertová D, Brewer P, Růžička K, Blilou I, Rouquié D,
Benková E, Scheres B, Friml J. 2006. Polar PIN localization directs auxin flow
in plants. Science. 312(5775).
mla: Wiśniewska, Justyna, et al. “Polar PIN Localization Directs Auxin Flow in Plants.”
Science, vol. 312, no. 5775, American Association for the Advancement of
Science, 2006, doi:10.1126/science.1121356.
short: J. Wiśniewska, J. Xu, D. Seifertová, P. Brewer, K. Růžička, I. Blilou, D.
Rouquié, E. Benková, B. Scheres, J. Friml, Science 312 (2006).
date_created: 2018-12-11T12:00:51Z
date_published: 2006-05-12T00:00:00Z
date_updated: 2021-01-12T07:40:27Z
day: '12'
doi: 10.1126/science.1121356
extern: 1
intvolume: ' 312'
issue: '5775'
month: '05'
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '3691'
quality_controlled: 0
status: public
title: Polar PIN localization directs auxin flow in plants
type: journal_article
volume: 312
year: '2006'
...