---
_id: '3934'
abstract:
- lang: eng
text: T cells develop in the thymus in a highly specialized cellular and extracellular
microenvironment. The basement membrane molecule, laminin-5 (LN-5), is predominantly
found in the medulla of the human thymic lobules. Using high-resolution light
microscopy, we show here that LN-5 is localized in a bi-membranous conduit-like
structure, together with other typical basement membrane components including
collagen type IV, nidogen and perlecan. Other interstitial matrix components,
such as fibrillin-1 or -2, tenascin-C or fibrillar collagen types, were also associated
with these structures. Three-dimensional (3D) confocal microscopy suggested a
tubular structure, whereas immunoelectron and transmission electron microscopy
showed that the core of these tubes contained fibrillar collagens enwrapped by
the LN-5-containing membrane. These medullary conduits are surrounded by thymic
epithelial cells, which in vitro were found to bind LN-5, but also fibrillin and
tenascin-C. Dendritic cells were also detected in close vicinity to the conduits.
Both of these stromal cell types express major histocompatibility complex (MHC)
class II molecules capable of antigen presentation. The conduits are connected
to blood vessels but, with an average diameter of 2 mum, they are too small to
transport cells. However, evidence is provided that smaller molecules such as
a 10 kDa dextran, but not large molecules (>500 kDa), can be transported in
the conduits. These results clearly demonstrate that a conduit system, which is
also known from secondary lymphatic organs such as lymph nodes and spleen, is
present in the medulla of the human thymus, and that it might serve to transport
small blood-borne molecules or chemokines to defined locations within the medulla.
author:
- first_name: Mihaela
full_name: Drumea-Mirancea, Mihaela
last_name: Drumea Mirancea
- first_name: Johannes
full_name: Wessels, Johannes T
last_name: Wessels
- first_name: Claudia
full_name: Müller, Claudia A
last_name: Müller
- first_name: Mike
full_name: Essl, Mike
last_name: Essl
- first_name: Johannes
full_name: Eble, Johannes A
last_name: Eble
- first_name: Eva
full_name: Tolosa, Eva
last_name: Tolosa
- first_name: Manuel
full_name: Koch, Manuel
last_name: Koch
- first_name: Dieter
full_name: Reinhardt, Dieter P
last_name: Reinhardt
- first_name: Michael K
full_name: Michael Sixt
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Lydia
full_name: Sorokin, Lydia
last_name: Sorokin
- first_name: York
full_name: Stierhof, York-Dieter
last_name: Stierhof
- first_name: Heinz
full_name: Schwarz, Heinz
last_name: Schwarz
- first_name: Gerd
full_name: Klein, Gerd
last_name: Klein
citation:
ama: 'Drumea Mirancea M, Wessels J, Müller C, et al. Characterization of a conduit
system containing laminin-5 in the human thymus: a potential transport system
for small molecules. Journal of Cell Science. 2006;119(Pt 7):1396-1405.
doi:10.1242/jcs.02840'
apa: 'Drumea Mirancea, M., Wessels, J., Müller, C., Essl, M., Eble, J., Tolosa,
E., … Klein, G. (2006). Characterization of a conduit system containing laminin-5
in the human thymus: a potential transport system for small molecules. Journal
of Cell Science. Company of Biologists. https://doi.org/10.1242/jcs.02840'
chicago: 'Drumea Mirancea, Mihaela, Johannes Wessels, Claudia Müller, Mike Essl,
Johannes Eble, Eva Tolosa, Manuel Koch, et al. “Characterization of a Conduit
System Containing Laminin-5 in the Human Thymus: A Potential Transport System
for Small Molecules.” Journal of Cell Science. Company of Biologists, 2006.
https://doi.org/10.1242/jcs.02840.'
ieee: 'M. Drumea Mirancea et al., “Characterization of a conduit system containing
laminin-5 in the human thymus: a potential transport system for small molecules,”
Journal of Cell Science, vol. 119, no. Pt 7. Company of Biologists, pp.
1396–1405, 2006.'
ista: 'Drumea Mirancea M, Wessels J, Müller C, Essl M, Eble J, Tolosa E, Koch M,
Reinhardt D, Sixt MK, Sorokin L, Stierhof Y, Schwarz H, Klein G. 2006. Characterization
of a conduit system containing laminin-5 in the human thymus: a potential transport
system for small molecules. Journal of Cell Science. 119(Pt 7), 1396–1405.'
mla: 'Drumea Mirancea, Mihaela, et al. “Characterization of a Conduit System Containing
Laminin-5 in the Human Thymus: A Potential Transport System for Small Molecules.”
Journal of Cell Science, vol. 119, no. Pt 7, Company of Biologists, 2006,
pp. 1396–405, doi:10.1242/jcs.02840.'
short: M. Drumea Mirancea, J. Wessels, C. Müller, M. Essl, J. Eble, E. Tolosa, M.
Koch, D. Reinhardt, M.K. Sixt, L. Sorokin, Y. Stierhof, H. Schwarz, G. Klein,
Journal of Cell Science 119 (2006) 1396–1405.
date_created: 2018-12-11T12:05:58Z
date_published: 2006-04-01T00:00:00Z
date_updated: 2021-01-12T07:53:18Z
day: '01'
doi: 10.1242/jcs.02840
extern: 1
intvolume: ' 119'
issue: Pt 7
month: '04'
page: 1396 - 1405
publication: Journal of Cell Science
publication_status: published
publisher: Company of Biologists
publist_id: '2192'
quality_controlled: 0
status: public
title: 'Characterization of a conduit system containing laminin-5 in the human thymus:
a potential transport system for small molecules'
type: journal_article
volume: 119
year: '2006'
...
---
_id: '3935'
abstract:
- lang: eng
text: Integrins regulate cell behavior through the assembly of multiprotein complexes
at the site of cell adhesion. Parvins are components of such a multiprotein complex.
They consist of three members (alpha-, beta-, and gamma-parvin), form a functional
complex with integrin-linked kinase (ILK) and PINCH, and link integrins to the
actin cytoskeleton. Whereas alpha- and beta-parvins are widely expressed, gamma-parvin
has been reported to be expressed in hematopoietic organs. In the present study,
we report the expression pattern of the parvins in hematopoietic cells and the
phenotypic analysis of gamma-parvin-deficient mice. Whereas alpha-parvin is not
expressed in hematopoietic cells, beta-parvin is only found in myeloid cells and
gamma-parvin is present in both cells of the myeloid and lymphoid lineages, where
it binds ILK. Surprisingly, loss of gamma-parvin expression had no effect on blood
cell differentiation, proliferation, and survival and no consequence for the T-cell-dependent
antibody response and lymphocyte and dendritic cell migration. These data indicate
that despite the high expression of gamma-parvin in hematopoietic cells it must
play a more subtle role for blood cell homeostasis.
author:
- first_name: Haiyan
full_name: Chu, Haiyan
last_name: Chu
- first_name: Ingo
full_name: Thievessen, Ingo
last_name: Thievessen
- first_name: Michael K
full_name: Michael Sixt
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Tim
full_name: Lämmermann, Tim
last_name: Lämmermann
- first_name: Ari
full_name: Waisman, Ari
last_name: Waisman
- first_name: Attila
full_name: Braun, Attila
last_name: Braun
- first_name: Angelika
full_name: Noegel, Angelika A
last_name: Noegel
- first_name: Reinhard
full_name: Fässler, Reinhard
last_name: Fässler
citation:
ama: Chu H, Thievessen I, Sixt MK, et al. γ-Parvin is dispensable for hematopoiesis,
leukocyte trafficking, and T-cell-dependent antibody response. Molecular and
Cellular Biology. 2006;26(5):1817-1825. doi:10.1128/MCB.26.5.1817-1825.2006
apa: Chu, H., Thievessen, I., Sixt, M. K., Lämmermann, T., Waisman, A., Braun, A.,
… Fässler, R. (2006). γ-Parvin is dispensable for hematopoiesis, leukocyte trafficking,
and T-cell-dependent antibody response. Molecular and Cellular Biology.
American Society for Microbiology. https://doi.org/10.1128/MCB.26.5.1817-1825.2006
chicago: Chu, Haiyan, Ingo Thievessen, Michael K Sixt, Tim Lämmermann, Ari Waisman,
Attila Braun, Angelika Noegel, and Reinhard Fässler. “γ-Parvin Is Dispensable
for Hematopoiesis, Leukocyte Trafficking, and T-Cell-Dependent Antibody Response.”
Molecular and Cellular Biology. American Society for Microbiology, 2006.
https://doi.org/10.1128/MCB.26.5.1817-1825.2006.
ieee: H. Chu et al., “γ-Parvin is dispensable for hematopoiesis, leukocyte
trafficking, and T-cell-dependent antibody response,” Molecular and Cellular
Biology, vol. 26, no. 5. American Society for Microbiology, pp. 1817–1825,
2006.
ista: Chu H, Thievessen I, Sixt MK, Lämmermann T, Waisman A, Braun A, Noegel A,
Fässler R. 2006. γ-Parvin is dispensable for hematopoiesis, leukocyte trafficking,
and T-cell-dependent antibody response. Molecular and Cellular Biology. 26(5),
1817–1825.
mla: Chu, Haiyan, et al. “γ-Parvin Is Dispensable for Hematopoiesis, Leukocyte Trafficking,
and T-Cell-Dependent Antibody Response.” Molecular and Cellular Biology,
vol. 26, no. 5, American Society for Microbiology, 2006, pp. 1817–25, doi:10.1128/MCB.26.5.1817-1825.2006.
short: H. Chu, I. Thievessen, M.K. Sixt, T. Lämmermann, A. Waisman, A. Braun, A.
Noegel, R. Fässler, Molecular and Cellular Biology 26 (2006) 1817–1825.
date_created: 2018-12-11T12:05:58Z
date_published: 2006-03-01T00:00:00Z
date_updated: 2021-01-12T07:53:18Z
day: '01'
doi: 10.1128/MCB.26.5.1817-1825.2006
extern: 1
intvolume: ' 26'
issue: '5'
month: '03'
page: 1817 - 1825
publication: Molecular and Cellular Biology
publication_status: published
publisher: American Society for Microbiology
publist_id: '2193'
quality_controlled: 0
status: public
title: γ-Parvin is dispensable for hematopoiesis, leukocyte trafficking, and T-cell-dependent
antibody response
type: journal_article
volume: 26
year: '2006'
...
---
_id: '3936'
abstract:
- lang: eng
text: At least eight of the twelve known members of the beta1 integrin family are
expressed on hematopoietic cells. Among these, the VCAM-1 receptor alpha4beta1
has received most attention as a main factor mediating firm adhesion to the endothelium
during blood cell extravasation. Therapeutic trials are ongoing into the use of
antibodies and small molecule inhibitors to target this interaction and hence
obtain anti-inflammatory effects. However, extravasation is only one possible
process that is mediated by beta1 integrins and there is evidence that they also
mediate leukocyte retention and positioning in the tissue, lymphocyte activation
and possibly migration within the interstitium. Genetic mouse models where integrins
are selectively deleted on blood cells have been used to investigate these functions
and further studies will be invaluable to critically evaluate therapeutic trials.
author:
- first_name: Michael K
full_name: Michael Sixt
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Martina
full_name: Bauer, Martina
last_name: Bauer
- first_name: Tim
full_name: Lämmermann, Tim
last_name: Lämmermann
- first_name: Reinhard
full_name: Fässler, Reinhard
last_name: Fässler
citation:
ama: 'Sixt MK, Bauer M, Lämmermann T, Fässler R. β1 integrins: zip codes and signaling
relay for blood cells. Current Opinion in Cell Biology. 2006;18(5):482-490.
doi:10.1016/j.ceb.2006.08.007'
apa: 'Sixt, M. K., Bauer, M., Lämmermann, T., & Fässler, R. (2006). β1 integrins:
zip codes and signaling relay for blood cells. Current Opinion in Cell Biology.
Elsevier. https://doi.org/10.1016/j.ceb.2006.08.007'
chicago: 'Sixt, Michael K, Martina Bauer, Tim Lämmermann, and Reinhard Fässler.
“Β1 Integrins: Zip Codes and Signaling Relay for Blood Cells.” Current Opinion
in Cell Biology. Elsevier, 2006. https://doi.org/10.1016/j.ceb.2006.08.007.'
ieee: 'M. K. Sixt, M. Bauer, T. Lämmermann, and R. Fässler, “β1 integrins: zip codes
and signaling relay for blood cells,” Current Opinion in Cell Biology,
vol. 18, no. 5. Elsevier, pp. 482–490, 2006.'
ista: 'Sixt MK, Bauer M, Lämmermann T, Fässler R. 2006. β1 integrins: zip codes
and signaling relay for blood cells. Current Opinion in Cell Biology. 18(5), 482–490.'
mla: 'Sixt, Michael K., et al. “Β1 Integrins: Zip Codes and Signaling Relay for
Blood Cells.” Current Opinion in Cell Biology, vol. 18, no. 5, Elsevier,
2006, pp. 482–90, doi:10.1016/j.ceb.2006.08.007.'
short: M.K. Sixt, M. Bauer, T. Lämmermann, R. Fässler, Current Opinion in Cell Biology
18 (2006) 482–490.
date_created: 2018-12-11T12:05:59Z
date_published: 2006-10-01T00:00:00Z
date_updated: 2021-01-12T07:53:19Z
day: '01'
doi: 10.1016/j.ceb.2006.08.007
extern: 1
intvolume: ' 18'
issue: '5'
month: '10'
page: 482 - 490
publication: Current Opinion in Cell Biology
publication_status: published
publisher: Elsevier
publist_id: '2191'
quality_controlled: 0
status: public
title: 'β1 integrins: zip codes and signaling relay for blood cells'
type: journal_article
volume: 18
year: '2006'
...
---
_id: '4140'
abstract:
- lang: eng
text: Wnt11 is a key signal, determining cell polarization and migration during
vertebrate gastrulation. It is known that Wnt11 functionally interacts with several
signaling components, the homologues of which control planar cell polarity in
Drosophila melanogaster. Although in D. melanogaster these components are thought
to polarize cells by asymmetrically localizing at the plasma membrane, it is not
yet clear whether their subcellular localization plays a similarly important role
in vertebrates. We show that in zebrafish embryonic cells, Wnt11 locally functions
at the plasma membrane by accumulating its receptor, Frizzled 7, on adjacent sites
of cell contacts. Wnt11-induced Frizzled 7 accumulations recruit the intracellular
Wnt signaling mediator Dishevelled, as well as Wnt11 itself, and locally increase
cell contact persistence. This increase in cell contact persistence is mediated
by the local interaction of Wnt11, Frizzled 7, and the atypical cadherin Flamingo
at the plasma membrane, and it does not require the activity of further downstream
effectors of Wnt11 signaling, such as RhoA and Rok2. We propose that Wnt11, by
interacting with Frizzled 7 and Flamingo, modulates local cell contact persistence
to coordinate cell movements during gastrulation.
article_processing_charge: No
author:
- first_name: Sabine
full_name: Witzel, Sabine
last_name: Witzel
- first_name: Vitaly
full_name: Zimyanin, Vitaly
last_name: Zimyanin
- first_name: Filipa
full_name: Carreira Barbosa, Filipa
last_name: Carreira Barbosa
- first_name: Masazumi
full_name: Tada, Masazumi
last_name: Tada
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Witzel S, Zimyanin V, Carreira Barbosa F, Tada M, Heisenberg C-PJ. Wnt11 controls
cell contact persistence by local accumulation of Frizzled 7 at the plasma membrane.
Journal of Cell Biology. 2006;175(5):791-802. doi:10.1083/jcb.200606017
apa: Witzel, S., Zimyanin, V., Carreira Barbosa, F., Tada, M., & Heisenberg,
C.-P. J. (2006). Wnt11 controls cell contact persistence by local accumulation
of Frizzled 7 at the plasma membrane. Journal of Cell Biology. Rockefeller
University Press. https://doi.org/10.1083/jcb.200606017
chicago: Witzel, Sabine, Vitaly Zimyanin, Filipa Carreira Barbosa, Masazumi Tada,
and Carl-Philipp J Heisenberg. “Wnt11 Controls Cell Contact Persistence by Local
Accumulation of Frizzled 7 at the Plasma Membrane.” Journal of Cell Biology.
Rockefeller University Press, 2006. https://doi.org/10.1083/jcb.200606017.
ieee: S. Witzel, V. Zimyanin, F. Carreira Barbosa, M. Tada, and C.-P. J. Heisenberg,
“Wnt11 controls cell contact persistence by local accumulation of Frizzled 7 at
the plasma membrane,” Journal of Cell Biology, vol. 175, no. 5. Rockefeller
University Press, pp. 791–802, 2006.
ista: Witzel S, Zimyanin V, Carreira Barbosa F, Tada M, Heisenberg C-PJ. 2006. Wnt11
controls cell contact persistence by local accumulation of Frizzled 7 at the plasma
membrane. Journal of Cell Biology. 175(5), 791–802.
mla: Witzel, Sabine, et al. “Wnt11 Controls Cell Contact Persistence by Local Accumulation
of Frizzled 7 at the Plasma Membrane.” Journal of Cell Biology, vol. 175,
no. 5, Rockefeller University Press, 2006, pp. 791–802, doi:10.1083/jcb.200606017.
short: S. Witzel, V. Zimyanin, F. Carreira Barbosa, M. Tada, C.-P.J. Heisenberg,
Journal of Cell Biology 175 (2006) 791–802.
date_created: 2018-12-11T12:07:11Z
date_published: 2006-12-04T00:00:00Z
date_updated: 2021-01-12T07:54:48Z
day: '04'
doi: 10.1083/jcb.200606017
extern: '1'
intvolume: ' 175'
issue: '5'
language:
- iso: eng
month: '12'
oa_version: None
page: 791 - 802
publication: Journal of Cell Biology
publication_status: published
publisher: Rockefeller University Press
publist_id: '1980'
status: public
title: Wnt11 controls cell contact persistence by local accumulation of Frizzled 7
at the plasma membrane
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 175
year: '2006'
...
---
_id: '4145'
abstract:
- lang: eng
text: The detection of microRNAs (miRNAs) at single-cell resolution is important
for studying the role of these posttranscriptional regulators. Here, we use a
dual-fluorescent green fluorescent protein (GFP)-reporter/monomeric red fluorescent
protein (mRFP)-sensor (DFRS) plasmid, injected into zebrafish blastomeres or electroporated
into defined tissues of mouse embryos in utero or ex utero, to monitor the dynamics
of specific miRNAs in individual live cells. This approach reveals, for example,
that in the developing mouse central nervous system,, miR-124a is expressed not
only in postmitotic neurons but also in neuronal progenitor cells. Collectively,
our results demonstrate that acute administration of DFRS plasmids.offers an alternative
to previous in situ hybridization and transgenic approaches and allows the monitoring
of miRNA appearance and disappearance in defined cell lineages during vertebrate
development.
article_processing_charge: No
author:
- first_name: Davide
full_name: Tonelli, Davide
last_name: Tonelli
- first_name: Frederico
full_name: Calegari, Frederico
last_name: Calegari
- first_name: Ji
full_name: Fei, Ji
last_name: Fei
- first_name: Tadashi
full_name: Nomura, Tadashi
last_name: Nomura
- first_name: Noriko
full_name: Osumi, Noriko
last_name: Osumi
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Wieland
full_name: Huttner, Wieland
last_name: Huttner
citation:
ama: Tonelli D, Calegari F, Fei J, et al. Single-cell detection of microRNAs in
developing vertebrate embryos after acute administration of a dual-fluorescence
reporter/sensor plasmid. Biotechniques. 2006;41(6):727-732. doi:10.2144/000112296
apa: Tonelli, D., Calegari, F., Fei, J., Nomura, T., Osumi, N., Heisenberg, C.-P.
J., & Huttner, W. (2006). Single-cell detection of microRNAs in developing
vertebrate embryos after acute administration of a dual-fluorescence reporter/sensor
plasmid. Biotechniques. Informa Healthcare. https://doi.org/10.2144/000112296
chicago: Tonelli, Davide, Frederico Calegari, Ji Fei, Tadashi Nomura, Noriko Osumi,
Carl-Philipp J Heisenberg, and Wieland Huttner. “Single-Cell Detection of MicroRNAs
in Developing Vertebrate Embryos after Acute Administration of a Dual-Fluorescence
Reporter/Sensor Plasmid.” Biotechniques. Informa Healthcare, 2006. https://doi.org/10.2144/000112296.
ieee: D. Tonelli et al., “Single-cell detection of microRNAs in developing
vertebrate embryos after acute administration of a dual-fluorescence reporter/sensor
plasmid,” Biotechniques, vol. 41, no. 6. Informa Healthcare, pp. 727–732,
2006.
ista: Tonelli D, Calegari F, Fei J, Nomura T, Osumi N, Heisenberg C-PJ, Huttner
W. 2006. Single-cell detection of microRNAs in developing vertebrate embryos after
acute administration of a dual-fluorescence reporter/sensor plasmid. Biotechniques.
41(6), 727–732.
mla: Tonelli, Davide, et al. “Single-Cell Detection of MicroRNAs in Developing Vertebrate
Embryos after Acute Administration of a Dual-Fluorescence Reporter/Sensor Plasmid.”
Biotechniques, vol. 41, no. 6, Informa Healthcare, 2006, pp. 727–32, doi:10.2144/000112296.
short: D. Tonelli, F. Calegari, J. Fei, T. Nomura, N. Osumi, C.-P.J. Heisenberg,
W. Huttner, Biotechniques 41 (2006) 727–732.
date_created: 2018-12-11T12:07:12Z
date_published: 2006-12-01T00:00:00Z
date_updated: 2021-01-12T07:54:50Z
day: '01'
doi: 10.2144/000112296
extern: '1'
intvolume: ' 41'
issue: '6'
language:
- iso: eng
month: '12'
oa_version: None
page: 727 - 732
publication: Biotechniques
publication_status: published
publisher: Informa Healthcare
publist_id: '1974'
status: public
title: Single-cell detection of microRNAs in developing vertebrate embryos after acute
administration of a dual-fluorescence reporter/sensor plasmid
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 41
year: '2006'
...
---
_id: '4176'
abstract:
- lang: eng
text: 'During vertebrate gastrulation, a well-orchestrated series of morphogenetic
changes leads to the formation of the three germ layers: the ectoderm, mesoderm
and endoderm. The analysis of gene expression patterns during gastrulation has
been central to the identification of genes involved in germ layer formation.
However, many proteins are regulated on a translational or post-translational
level and are thus undetectable by gene expression analysis. Therefore, we developed
a 2D-gel-based comparative proteomic approach to target proteins involved in germ
layer morphogenesis during zebrafish gastrulation. Proteomes of ectodermal and
mesendodermal progenitor cells were compared and 35 significantly regulated proteins
were identified by mass spectrometry, including several proteins with predicted
functions in cytoskeletal organization. A comparison of our proteomic results
with data obtained in an accompanying microarray-based gene expression analysis
revealed no significant overlap, confirming the complementary nature of proteomics
and transcriptomics. The regulation of ezrin2, which was identified based on a
reduction in spot intensity in mesendodermal cells, was independently validated.
Furthermore, we show that ezrin2 is activated by phosphorylation in mesendodermal
cells and is required for proper germ layer morphogenesis. We demonstrate the
feasibility of proteomics in zebrafish, concluding that proteomics is a valuable
tool for analysis of early development.'
article_processing_charge: No
author:
- first_name: Vinzenz
full_name: Link, Vinzenz
last_name: Link
- first_name: Lara
full_name: Carvalho, Lara
last_name: Carvalho
- first_name: Irinka
full_name: Castanon, Irinka
last_name: Castanon
- first_name: Petra
full_name: Stockinger, Petra
last_name: Stockinger
- first_name: Andrej
full_name: Shevchenko, Andrej
last_name: Shevchenko
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Link V, Carvalho L, Castanon I, Stockinger P, Shevchenko A, Heisenberg C-PJ.
Identification of regulators of germ layer morphogenesis using proteomics in zebrafish.
Journal of Cell Science. 2006;119(10):2073-2083. doi:10.1242/jcs.02928
apa: Link, V., Carvalho, L., Castanon, I., Stockinger, P., Shevchenko, A., &
Heisenberg, C.-P. J. (2006). Identification of regulators of germ layer morphogenesis
using proteomics in zebrafish. Journal of Cell Science. Company of Biologists.
https://doi.org/10.1242/jcs.02928
chicago: Link, Vinzenz, Lara Carvalho, Irinka Castanon, Petra Stockinger, Andrej
Shevchenko, and Carl-Philipp J Heisenberg. “Identification of Regulators of Germ
Layer Morphogenesis Using Proteomics in Zebrafish.” Journal of Cell Science.
Company of Biologists, 2006. https://doi.org/10.1242/jcs.02928.
ieee: V. Link, L. Carvalho, I. Castanon, P. Stockinger, A. Shevchenko, and C.-P.
J. Heisenberg, “Identification of regulators of germ layer morphogenesis using
proteomics in zebrafish,” Journal of Cell Science, vol. 119, no. 10. Company
of Biologists, pp. 2073–2083, 2006.
ista: Link V, Carvalho L, Castanon I, Stockinger P, Shevchenko A, Heisenberg C-PJ.
2006. Identification of regulators of germ layer morphogenesis using proteomics
in zebrafish. Journal of Cell Science. 119(10), 2073–2083.
mla: Link, Vinzenz, et al. “Identification of Regulators of Germ Layer Morphogenesis
Using Proteomics in Zebrafish.” Journal of Cell Science, vol. 119, no.
10, Company of Biologists, 2006, pp. 2073–83, doi:10.1242/jcs.02928.
short: V. Link, L. Carvalho, I. Castanon, P. Stockinger, A. Shevchenko, C.-P.J.
Heisenberg, Journal of Cell Science 119 (2006) 2073–2083.
date_created: 2018-12-11T12:07:24Z
date_published: 2006-05-15T00:00:00Z
date_updated: 2021-01-12T07:55:04Z
day: '15'
doi: 10.1242/jcs.02928
extern: '1'
intvolume: ' 119'
issue: '10'
language:
- iso: eng
month: '05'
oa_version: None
page: 2073 - 2083
publication: Journal of Cell Science
publication_status: published
publisher: Company of Biologists
publist_id: '1944'
status: public
title: Identification of regulators of germ layer morphogenesis using proteomics in
zebrafish
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 119
year: '2006'
...
---
_id: '4173'
abstract:
- lang: eng
text: 'Background: Zebrafish (D. rerio) has become a powerful and widely used model
system for the analysis of vertebrate embryogenesis and organ development. While
genetic methods are readily available in zebrafish, protocols for two dimensional
(2D) gel electrophoresis and proteomics have yet to be developed. Results: As
a prerequisite to carry out proteomic experiments with early zebrafish embryos,
we developed a method to efficiently remove the yolk from large batches of embryos.
This method enabled high resolution 2D gel electrophoresis and improved Western
blotting considerably. Here, we provide detailed protocols for proteomics in zebrafish
from sample preparation to mass spectrometry (MS), including a comparison of databases
for MS identification of zebrafish proteins. Conclusion: The provided protocols
for proteomic analysis of early embryos enable research to be taken in novel directions
in embryogenesis.'
article_processing_charge: No
author:
- first_name: Vinzenz
full_name: Link, Vinzenz
last_name: Link
- first_name: Andrej
full_name: Shevchenko, Andrej
last_name: Shevchenko
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Link V, Shevchenko A, Heisenberg C-PJ. Proteomics of early zebrafish embryos.
BMC Developmental Biology. 2006;6:1-9. doi:10.1186/1471-213X-6-1
apa: Link, V., Shevchenko, A., & Heisenberg, C.-P. J. (2006). Proteomics of
early zebrafish embryos. BMC Developmental Biology. BioMed Central. https://doi.org/10.1186/1471-213X-6-1
chicago: Link, Vinzenz, Andrej Shevchenko, and Carl-Philipp J Heisenberg. “Proteomics
of Early Zebrafish Embryos.” BMC Developmental Biology. BioMed Central,
2006. https://doi.org/10.1186/1471-213X-6-1.
ieee: V. Link, A. Shevchenko, and C.-P. J. Heisenberg, “Proteomics of early zebrafish
embryos,” BMC Developmental Biology, vol. 6. BioMed Central, pp. 1–9, 2006.
ista: Link V, Shevchenko A, Heisenberg C-PJ. 2006. Proteomics of early zebrafish
embryos. BMC Developmental Biology. 6, 1–9.
mla: Link, Vinzenz, et al. “Proteomics of Early Zebrafish Embryos.” BMC Developmental
Biology, vol. 6, BioMed Central, 2006, pp. 1–9, doi:10.1186/1471-213X-6-1.
short: V. Link, A. Shevchenko, C.-P.J. Heisenberg, BMC Developmental Biology 6 (2006)
1–9.
date_created: 2018-12-11T12:07:23Z
date_published: 2006-01-13T00:00:00Z
date_updated: 2021-01-12T07:55:02Z
day: '13'
doi: 10.1186/1471-213X-6-1
extern: '1'
intvolume: ' 6'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
main_file_link:
- open_access: '1'
url: http://www.biomedcentral.com/1471-213X/6/1
month: '01'
oa: 1
oa_version: None
page: 1 - 9
publication: BMC Developmental Biology
publication_status: published
publisher: BioMed Central
publist_id: '1945'
status: public
title: Proteomics of early zebrafish embryos
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 6
year: '2006'
...
---
_id: '4178'
abstract:
- lang: eng
text: Detailed reconstruction of the spatiotemporal history of embryonic cells is
key to understanding tissue formation processes but is often complicated by the
large number of cells involved, particularly so in vertebrates. Through a combination
of high-resolution time-lapse lineage tracing and antibody staining, we have analyzed
the movement of mesencephalic and metencephalic cell populations in the early
zebrafish embryo. To facilitate the analysis of our cell tracking data, we have
created TracePilot, a software tool that allows interactive manipulation and visualization
of tracking data. We demonstrate its utility by showing novel visualizations of
cell movement in the developing zebrafish brain. TracePilot (http://www.mpi-cbg.de/tracepilot)
is Java-based, available free of charge, and has a program structure that allows
the incorporation of additional analysis tools.
article_processing_charge: No
author:
- first_name: Tobias
full_name: Langenberg, Tobias
last_name: Langenberg
- first_name: Tadeusz
full_name: Dracz, Tadeusz
last_name: Dracz
- first_name: Andrew
full_name: Oates, Andrew
last_name: Oates
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Michael
full_name: Brand, Michael
last_name: Brand
citation:
ama: Langenberg T, Dracz T, Oates A, Heisenberg C-PJ, Brand M. Analysis and visualization
of cell movement in the developing zebrafish brain. Developmental Dynamics.
2006;235(4):928-933. doi:10.1002/dvdy.20692
apa: Langenberg, T., Dracz, T., Oates, A., Heisenberg, C.-P. J., & Brand, M.
(2006). Analysis and visualization of cell movement in the developing zebrafish
brain. Developmental Dynamics. Wiley-Blackwell. https://doi.org/10.1002/dvdy.20692
chicago: Langenberg, Tobias, Tadeusz Dracz, Andrew Oates, Carl-Philipp J Heisenberg,
and Michael Brand. “Analysis and Visualization of Cell Movement in the Developing
Zebrafish Brain.” Developmental Dynamics. Wiley-Blackwell, 2006. https://doi.org/10.1002/dvdy.20692.
ieee: T. Langenberg, T. Dracz, A. Oates, C.-P. J. Heisenberg, and M. Brand, “Analysis
and visualization of cell movement in the developing zebrafish brain,” Developmental
Dynamics, vol. 235, no. 4. Wiley-Blackwell, pp. 928–933, 2006.
ista: Langenberg T, Dracz T, Oates A, Heisenberg C-PJ, Brand M. 2006. Analysis and
visualization of cell movement in the developing zebrafish brain. Developmental
Dynamics. 235(4), 928–933.
mla: Langenberg, Tobias, et al. “Analysis and Visualization of Cell Movement in
the Developing Zebrafish Brain.” Developmental Dynamics, vol. 235, no.
4, Wiley-Blackwell, 2006, pp. 928–33, doi:10.1002/dvdy.20692.
short: T. Langenberg, T. Dracz, A. Oates, C.-P.J. Heisenberg, M. Brand, Developmental
Dynamics 235 (2006) 928–933.
date_created: 2018-12-11T12:07:25Z
date_published: 2006-04-01T00:00:00Z
date_updated: 2021-01-12T07:55:04Z
day: '01'
doi: 10.1002/dvdy.20692
extern: '1'
intvolume: ' 235'
issue: '4'
language:
- iso: eng
month: '04'
oa_version: None
page: 928 - 933
publication: Developmental Dynamics
publication_status: published
publisher: Wiley-Blackwell
publist_id: '1940'
status: public
title: Analysis and visualization of cell movement in the developing zebrafish brain
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 235
year: '2006'
...
---
_id: '4184'
abstract:
- lang: eng
text: Epithelial morphogenesis depends on coordinated changes in cell shape, a process
that is still poorly understood. During zebrafish epiboly and Drosophila dorsal
closure, cell-shape changes at the epithelial margin are of critical importance.
Here evidence is provided for a conserved mechanism of local actin and myosin
2 recruitment during theses events. It was found that during epiboly of the zebrafish
embryo, the movement of the outer epithelium (enveloping layer) over the yolk
cell surface involves the constriction of marginal cells. This process depends
on the recruitment of actin and myosin 2 within the yolk cytoplasm along the margin
of the enveloping layer. Actin and myosin 2 recruitment within the yolk cytoplasm
requires the Ste20-like kinase Msn1, an orthologue of Drosophila Misshapen. Similarly,
in Drosophila, actin and myosin 2 localization and cell constriction at the margin
of the epidermis mediate dorsal closure and are controlled by Misshapen. Thus,
this study has characterized a conserved mechanism underlying coordinated cell-shape
changes during epithelial morphogenesis.
article_processing_charge: No
author:
- first_name: Mathias
full_name: Köppen, Mathias
last_name: Köppen
- first_name: Beatriz
full_name: Fernández, Beatriz
last_name: Fernández
- first_name: Lara
full_name: Carvalho, Lara
last_name: Carvalho
- first_name: António
full_name: Jacinto, António
last_name: Jacinto
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: 'Köppen M, Fernández B, Carvalho L, Jacinto A, Heisenberg C-PJ. Coordinated
cell-shape changes control epithelial movement in zebrafish and Drosophila. Development.
2006;133(14):2671-2681. doi:doi:
10.1242/dev.02439'
apa: 'Köppen, M., Fernández, B., Carvalho, L., Jacinto, A., & Heisenberg, C.-P.
J. (2006). Coordinated cell-shape changes control epithelial movement in zebrafish
and Drosophila. Development. Company of Biologists. https://doi.org/doi: 10.1242/dev.02439'
chicago: 'Köppen, Mathias, Beatriz Fernández, Lara Carvalho, António Jacinto, and
Carl-Philipp J Heisenberg. “Coordinated Cell-Shape Changes Control Epithelial
Movement in Zebrafish and Drosophila.” Development. Company of Biologists,
2006. https://doi.org/doi: 10.1242/dev.02439.'
ieee: M. Köppen, B. Fernández, L. Carvalho, A. Jacinto, and C.-P. J. Heisenberg,
“Coordinated cell-shape changes control epithelial movement in zebrafish and Drosophila,”
Development, vol. 133, no. 14. Company of Biologists, pp. 2671–2681, 2006.
ista: Köppen M, Fernández B, Carvalho L, Jacinto A, Heisenberg C-PJ. 2006. Coordinated
cell-shape changes control epithelial movement in zebrafish and Drosophila. Development.
133(14), 2671–2681.
mla: 'Köppen, Mathias, et al. “Coordinated Cell-Shape Changes Control Epithelial
Movement in Zebrafish and Drosophila.” Development, vol. 133, no. 14, Company
of Biologists, 2006, pp. 2671–81, doi:doi:
10.1242/dev.02439.'
short: M. Köppen, B. Fernández, L. Carvalho, A. Jacinto, C.-P.J. Heisenberg, Development
133 (2006) 2671–2681.
date_created: 2018-12-11T12:07:27Z
date_published: 2006-07-15T00:00:00Z
date_updated: 2021-01-12T07:55:08Z
day: '15'
doi: 'doi: 10.1242/dev.02439'
extern: '1'
intvolume: ' 133'
issue: '14'
language:
- iso: eng
month: '07'
oa_version: None
page: 2671 - 2681
publication: Development
publication_status: published
publisher: Company of Biologists
publist_id: '1935'
status: public
title: Coordinated cell-shape changes control epithelial movement in zebrafish and
Drosophila
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 133
year: '2006'
...
---
_id: '4218'
abstract:
- lang: eng
text: The molecular and cellular mechanisms governing cell motility and directed
migration in response to the chemokine SDF-1 are largely unknown. Here, we demonstrate
that zebrafish primordial germ cells whose migration is guided by SDF-1 generate
bleb-like protrusions that are powered by cytoplasmic flow. Protrusions are formed
at sites of higher levels of free calcium where activation of myosin contraction
occurs. Separation of the acto-myosin cortex from the plasma membrane at these
sites is followed by a flow of cytoplasm into the forming bleb. We propose that
polarized activation of the receptor CXCR4 leads to a rise in free calcium that
in turn activates myosin contraction in the part of the cell responding to higher
levels of the ligand SDF-1. The biased formation of new protrusions in a particular
region of the cell in response to SDF-1 defines the leading edge and the direction
of cell migration.
article_processing_charge: No
author:
- first_name: Heiko
full_name: Blaser, Heiko
last_name: Blaser
- first_name: Michal
full_name: Reichman Fried, Michal
last_name: Reichman Fried
- first_name: Irinka
full_name: Castanon, Irinka
last_name: Castanon
- first_name: Karin
full_name: Dumstrei, Karin
last_name: Dumstrei
- first_name: Florence
full_name: Marlow, Florence
last_name: Marlow
- first_name: Koichi
full_name: Kawakami, Koichi
last_name: Kawakami
- first_name: Lilianna
full_name: Solnica Krezel, Lilianna
last_name: Solnica Krezel
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Erez
full_name: Raz, Erez
last_name: Raz
citation:
ama: 'Blaser H, Reichman Fried M, Castanon I, et al. Migration of zebrafish primordial
germ cells: A role for myosin contraction and cytoplasmic flow. Developmental
Cell. 2006;11(5):613-627. doi:10.1016/j.devcel.2006.09.023'
apa: 'Blaser, H., Reichman Fried, M., Castanon, I., Dumstrei, K., Marlow, F., Kawakami,
K., … Raz, E. (2006). Migration of zebrafish primordial germ cells: A role for
myosin contraction and cytoplasmic flow. Developmental Cell. Cell Press.
https://doi.org/10.1016/j.devcel.2006.09.023'
chicago: 'Blaser, Heiko, Michal Reichman Fried, Irinka Castanon, Karin Dumstrei,
Florence Marlow, Koichi Kawakami, Lilianna Solnica Krezel, Carl-Philipp J Heisenberg,
and Erez Raz. “Migration of Zebrafish Primordial Germ Cells: A Role for Myosin
Contraction and Cytoplasmic Flow.” Developmental Cell. Cell Press, 2006.
https://doi.org/10.1016/j.devcel.2006.09.023.'
ieee: 'H. Blaser et al., “Migration of zebrafish primordial germ cells: A
role for myosin contraction and cytoplasmic flow,” Developmental Cell,
vol. 11, no. 5. Cell Press, pp. 613–627, 2006.'
ista: 'Blaser H, Reichman Fried M, Castanon I, Dumstrei K, Marlow F, Kawakami K,
Solnica Krezel L, Heisenberg C-PJ, Raz E. 2006. Migration of zebrafish primordial
germ cells: A role for myosin contraction and cytoplasmic flow. Developmental
Cell. 11(5), 613–627.'
mla: 'Blaser, Heiko, et al. “Migration of Zebrafish Primordial Germ Cells: A Role
for Myosin Contraction and Cytoplasmic Flow.” Developmental Cell, vol.
11, no. 5, Cell Press, 2006, pp. 613–27, doi:10.1016/j.devcel.2006.09.023.'
short: H. Blaser, M. Reichman Fried, I. Castanon, K. Dumstrei, F. Marlow, K. Kawakami,
L. Solnica Krezel, C.-P.J. Heisenberg, E. Raz, Developmental Cell 11 (2006) 613–627.
date_created: 2018-12-11T12:07:39Z
date_published: 2006-11-06T00:00:00Z
date_updated: 2021-01-12T07:55:23Z
day: '06'
doi: 10.1016/j.devcel.2006.09.023
extern: '1'
intvolume: ' 11'
issue: '5'
language:
- iso: eng
month: '11'
oa_version: None
page: 613 - 627
publication: Developmental Cell
publication_status: published
publisher: Cell Press
publist_id: '1898'
status: public
title: 'Migration of zebrafish primordial germ cells: A role for myosin contraction
and cytoplasmic flow'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 11
year: '2006'
...