[{"doi":"10.1002/cne.20160","date_published":"2004-07-12T00:00:00Z","page":"36 - 46","quality_controlled":0,"citation":{"ama":"Luján R, Shigemoto R, Kulik Á, Juíz J. Localization of the GABAB receptor 1a/b subunit relative to glutamatergic synapses in the dorsal cochlear nucleus of the rat. Journal of Comparative Neurology. 2004;475(1):36-46. doi:10.1002/cne.20160","ista":"Luján R, Shigemoto R, Kulik Á, Juíz J. 2004. Localization of the GABAB receptor 1a/b subunit relative to glutamatergic synapses in the dorsal cochlear nucleus of the rat. Journal of Comparative Neurology. 475(1), 36–46.","ieee":"R. Luján, R. Shigemoto, Á. Kulik, and J. Juíz, “Localization of the GABAB receptor 1a/b subunit relative to glutamatergic synapses in the dorsal cochlear nucleus of the rat,” Journal of Comparative Neurology, vol. 475, no. 1. Wiley-Blackwell, pp. 36–46, 2004.","apa":"Luján, R., Shigemoto, R., Kulik, Á., & Juíz, J. (2004). Localization of the GABAB receptor 1a/b subunit relative to glutamatergic synapses in the dorsal cochlear nucleus of the rat. Journal of Comparative Neurology. Wiley-Blackwell. https://doi.org/10.1002/cne.20160","mla":"Luján, Rafael, et al. “Localization of the GABAB Receptor 1a/b Subunit Relative to Glutamatergic Synapses in the Dorsal Cochlear Nucleus of the Rat.” Journal of Comparative Neurology, vol. 475, no. 1, Wiley-Blackwell, 2004, pp. 36–46, doi:10.1002/cne.20160.","short":"R. Luján, R. Shigemoto, Á. Kulik, J. Juíz, Journal of Comparative Neurology 475 (2004) 36–46.","chicago":"Luján, Rafael, Ryuichi Shigemoto, Ákos Kulik, and José Juíz. “Localization of the GABAB Receptor 1a/b Subunit Relative to Glutamatergic Synapses in the Dorsal Cochlear Nucleus of the Rat.” Journal of Comparative Neurology. Wiley-Blackwell, 2004. https://doi.org/10.1002/cne.20160."},"publication":"Journal of Comparative Neurology","day":"12","month":"07","volume":475,"date_created":"2018-12-11T11:58:50Z","date_updated":"2021-01-12T06:58:46Z","author":[{"full_name":"Luján, Rafael","first_name":"Rafael","last_name":"Luján"},{"full_name":"Ryuichi Shigemoto","first_name":"Ryuichi","last_name":"Shigemoto","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8761-9444"},{"first_name":"Ákos","last_name":"Kulik","full_name":"Kulik, Ákos"},{"first_name":"José","last_name":"Juíz","full_name":"Juíz, José M"}],"publisher":"Wiley-Blackwell","intvolume":" 475","status":"public","publication_status":"published","title":"Localization of the GABAB receptor 1a/b subunit relative to glutamatergic synapses in the dorsal cochlear nucleus of the rat","year":"2004","_id":"2643","extern":1,"issue":"1","publist_id":"4254","abstract":[{"lang":"eng","text":"Metabotropic γ-aminobutyric acid receptors (GABAB) are involved in pre- and postsynaptic inhibitory effects upon auditory neurons and have been implicated in different aspects of acoustic information processing. To understand better the mechanisms by which GABAB receptors mediate their inhibitory effects, we used pre-embedding immunocytochemical techniques combined with quantification of immunogold particles to reveal the precise subcellular distribution of the GABAB1 subunit in the rat dorsal cochlear nucleus. At the light microscopic level, GABAB1 was detected in all divisions of the cochlear complex. The most intense immunoreactivity for GABAB1 was found in the dorsal cochlear nucleus, whereas immunoreactivity in the anteroventral and posteroventral cochlear nuclei was very low. In the dorsal cochlear nucleus, a punctate labeling was observed in the superficial (molecular and fusiform cell) layers. At the electron microscopic level, GABAB1 was found at both post- and presynaptic locations. Postsynaptically, GABAB1 was localized mainly in the dendritic spines of presumed fusiform cells. Quantitative immunogold immunocytochemistry revealed that the highest concentration of GABA B1 in the plasma membrane was in dendritic spines, followed by dendritic shafts and somata. Thus, the most intense immunoreactivity for GABAB1 was observed in dendritic spines with a high density of immunogold particles at extrasynaptic sites, peaking around 300 nm from glutamatergic synapses. This is in contrast to GABAergic synapses, in which GABAB1 was only occasionally found. Presynaptically, receptor immunoreactivity was detected primarily in axospinous endings, probably from granule cells, in both the active zone and extrasynaptic sites. The localization of GABAB1 relative to synaptic sites in the DCN suggests a role for the receptor in the regulation of dendritic excitability and excitatory inputs."}],"type":"journal_article"},{"doi":"10.1111/j.0953-816X.2004.03319.x","date_published":"2004-04-01T00:00:00Z","page":"2169 - 2178","quality_controlled":0,"citation":{"ama":"Kulik Á, Nakadate K, Hagiwara A, et al. Immunocytochemical localization of the α1A subunit of the P/Q-type calcium channel in the rat cerebellum. European Journal of Neuroscience. 2004;19(8):2169-2178. doi:10.1111/j.0953-816X.2004.03319.x","ista":"Kulik Á, Nakadate K, Hagiwara A, Fukazawa Y, Luján R, Saito H, Suzuki N, Futatsugi A, Mikoshiba K, Frotscher M, Shigemoto R. 2004. Immunocytochemical localization of the α1A subunit of the P/Q-type calcium channel in the rat cerebellum. European Journal of Neuroscience. 19(8), 2169–2178.","ieee":"Á. Kulik et al., “Immunocytochemical localization of the α1A subunit of the P/Q-type calcium channel in the rat cerebellum,” European Journal of Neuroscience, vol. 19, no. 8. Wiley-Blackwell, pp. 2169–2178, 2004.","apa":"Kulik, Á., Nakadate, K., Hagiwara, A., Fukazawa, Y., Luján, R., Saito, H., … Shigemoto, R. (2004). Immunocytochemical localization of the α1A subunit of the P/Q-type calcium channel in the rat cerebellum. European Journal of Neuroscience. Wiley-Blackwell. https://doi.org/10.1111/j.0953-816X.2004.03319.x","mla":"Kulik, Ákos, et al. “Immunocytochemical Localization of the Α1A Subunit of the P/Q-Type Calcium Channel in the Rat Cerebellum.” European Journal of Neuroscience, vol. 19, no. 8, Wiley-Blackwell, 2004, pp. 2169–78, doi:10.1111/j.0953-816X.2004.03319.x.","short":"Á. Kulik, K. Nakadate, A. Hagiwara, Y. Fukazawa, R. Luján, H. Saito, N. Suzuki, A. Futatsugi, K. Mikoshiba, M. Frotscher, R. Shigemoto, European Journal of Neuroscience 19 (2004) 2169–2178.","chicago":"Kulik, Ákos, Kazuhiko Nakadate, Akari Hagiwara, Yugo Fukazawa, Rafael Luján, Hiromitsu Saito, Noboru Suzuki, et al. “Immunocytochemical Localization of the Α1A Subunit of the P/Q-Type Calcium Channel in the Rat Cerebellum.” European Journal of Neuroscience. Wiley-Blackwell, 2004. https://doi.org/10.1111/j.0953-816X.2004.03319.x."},"publication":"European Journal of Neuroscience","day":"01","month":"04","volume":19,"date_updated":"2021-01-12T06:58:44Z","date_created":"2018-12-11T11:58:48Z","author":[{"last_name":"Kulik","first_name":"Ákos","full_name":"Kulik, Ákos"},{"full_name":"Nakadate, Kazuhiko","first_name":"Kazuhiko","last_name":"Nakadate"},{"full_name":"Hagiwara, Akari","first_name":"Akari","last_name":"Hagiwara"},{"full_name":"Fukazawa, Yugo","first_name":"Yugo","last_name":"Fukazawa"},{"full_name":"Luján, Rafael","last_name":"Luján","first_name":"Rafael"},{"first_name":"Hiromitsu","last_name":"Saito","full_name":"Saito, Hiromitsu"},{"full_name":"Suzuki, Noboru","last_name":"Suzuki","first_name":"Noboru"},{"last_name":"Futatsugi","first_name":"Akira","full_name":"Futatsugi, Akira"},{"full_name":"Mikoshiba, Katsuhiko","first_name":"Katsuhiko","last_name":"Mikoshiba"},{"full_name":"Frotscher, Michael","last_name":"Frotscher","first_name":"Michael"},{"full_name":"Ryuichi Shigemoto","orcid":"0000-0001-8761-9444","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","last_name":"Shigemoto","first_name":"Ryuichi"}],"publisher":"Wiley-Blackwell","intvolume":" 19","status":"public","publication_status":"published","title":"Immunocytochemical localization of the α1A subunit of the P/Q-type calcium channel in the rat cerebellum","year":"2004","_id":"2638","extern":1,"publist_id":"4259","issue":"8","abstract":[{"lang":"eng","text":"Among various types of low- and high-threshold calcium channels, the high voltage-activated P/Q-type channel is the most abundant in the cerebellum. These P/Q-type channels are involved in the regulation of neurotransmitter release and in the integration of dendritic inputs. We used an antibody specific for the α1A subunit of the P/Q-type channel in quantitative pre-embedding immunogold labelling combined with three-dimensional reconstruction to reveal the subcellular distribution of pre- and postsynaptic P/Q-type channels in the rat cerebellum. At the light microscopic level, immunoreactivity for the α1A protein was prevalent in the molecular layer, whereas immunostaining was moderate in the somata of Purkinje cells and weak in the granule cell layer. At the electron microscopic level, the most intense Immunoreactivity for the α1A subunit was found in the presynaptic active zone of parallel fibre varicosities. The dendritic spines of Purkinje cells were also strongly labelled with the highest density of immunoparticles detected within 180 nm from the edge of the asymmetrical parallel fibre-Purkinje cell synapses. By contrast, the immunolabelling was sparse in climbing fibre varicosities and axon terminals of GABAergic cells, and weak and diffuse in dendritic shafts of Purkinje cells. The association of the α1A subunit with the glutamatergic parallel fibre-Purkinje cell synapses suggests that presynaptic channels have a major role in the mediation of excitatory neurotransmission, whereas postsynaptic channels are likely to be involved in depolarization-induced generation of local calcium transients in Purkinje cells."}],"type":"journal_article"},{"type":"journal_article","abstract":[{"text":"Hyperpolarization-activated cation currents (Ih) contribute to various physiological properties and functions in the brain, including neuronal pacemaker activity, setting of resting membrane potential, and dendritic integration of synaptic input. Four subunits of the Hyperpolarization-activated and Cyclic-Nucleotide-gated nonselective cation channels (HCN1-4), which generate Ih, have been cloned recently. To better understand the functional diversity of Ih in the brain, we examined precise immunohistochemical localization of four HCNs in the rat brain. Immunoreactivity for HCN1 showed predominantly cortical distribution, being intense in the neocortex, hippocampus, superior colliculus, and cerebellum, whereas those for HCN3 and HCN4 exhibited subcortical distribution mainly concentrated in the hypothalamus and thalamus, respectively. Immunoreactivity for HCN2 had a widespread distribution throughout the brain. Double immunofluorescence revealed colocalization of immunoreactivity for HCN1 and HCN2 in distal dendrites of pyramidal cells in the hippocampus and neocortex. At the electron microscopic level, immunogold particles for HCN1 and HCN2 had similar distribution patterns along plasma membrane of dendritic shafts in layer I of the neocortex and stratum lacunosum moleculare of the hippocampal CA1 area, suggesting that these subunits could form heteromeric channels. Our results further indicate that HCNs are localized not only in somato-dendritic compartments but also in axonal compartments of neurons. Immunoreactivity for HCNs often occurred in preterminal rather than terminal portions of axons and in specific populations of myelinated axons. We also found HCN2-immunopositive oligodendrocytes including perineuronal oligodendrocytes throughout the brain. These results support previous electrophysiological findings and further suggest unexpected roles of Ih channels in the brain.","lang":"eng"}],"issue":"3","publist_id":"4258","extern":1,"_id":"2640","year":"2004","title":"Immunohistochemical localization of Ih channel subunits, HCN1-4, in the rat brain","publication_status":"published","status":"public","intvolume":" 471","publisher":"Wiley-Blackwell","author":[{"first_name":"Takuya","last_name":"Notomi","full_name":"Notomi, Takuya"},{"last_name":"Shigemoto","first_name":"Ryuichi","orcid":"0000-0001-8761-9444","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","full_name":"Ryuichi Shigemoto"}],"date_updated":"2021-01-12T06:58:45Z","date_created":"2018-12-11T11:58:49Z","volume":471,"day":"05","month":"04","publication":"Journal of Comparative Neurology","citation":{"apa":"Notomi, T., & Shigemoto, R. (2004). Immunohistochemical localization of Ih channel subunits, HCN1-4, in the rat brain. Journal of Comparative Neurology. Wiley-Blackwell. https://doi.org/10.1002/cne.11039","ieee":"T. Notomi and R. Shigemoto, “Immunohistochemical localization of Ih channel subunits, HCN1-4, in the rat brain,” Journal of Comparative Neurology, vol. 471, no. 3. Wiley-Blackwell, pp. 241–276, 2004.","ista":"Notomi T, Shigemoto R. 2004. Immunohistochemical localization of Ih channel subunits, HCN1-4, in the rat brain. Journal of Comparative Neurology. 471(3), 241–276.","ama":"Notomi T, Shigemoto R. Immunohistochemical localization of Ih channel subunits, HCN1-4, in the rat brain. Journal of Comparative Neurology. 2004;471(3):241-276. doi:10.1002/cne.11039","chicago":"Notomi, Takuya, and Ryuichi Shigemoto. “Immunohistochemical Localization of Ih Channel Subunits, HCN1-4, in the Rat Brain.” Journal of Comparative Neurology. Wiley-Blackwell, 2004. https://doi.org/10.1002/cne.11039.","short":"T. Notomi, R. Shigemoto, Journal of Comparative Neurology 471 (2004) 241–276.","mla":"Notomi, Takuya, and Ryuichi Shigemoto. “Immunohistochemical Localization of Ih Channel Subunits, HCN1-4, in the Rat Brain.” Journal of Comparative Neurology, vol. 471, no. 3, Wiley-Blackwell, 2004, pp. 241–76, doi:10.1002/cne.11039."},"quality_controlled":0,"page":"241 - 276","date_published":"2004-04-05T00:00:00Z","doi":"10.1002/cne.11039"},{"intvolume":" 24","publisher":"Society for Neuroscience","title":"Bidirectional interactions between H-channels and Na+-K + pumps in mesencephalic trigeminal neurons","status":"public","publication_status":"published","year":"2004","_id":"2641","volume":24,"date_created":"2018-12-11T11:58:49Z","date_updated":"2021-01-12T06:58:45Z","author":[{"full_name":"Kang, Youngnam","first_name":"Youngnam","last_name":"Kang"},{"first_name":"Takuya","last_name":"Notomi","full_name":"Notomi, Takuya"},{"full_name":"Saito, Mitsuru","first_name":"Mitsuru","last_name":"Saito"},{"full_name":"Zhang, Wei","last_name":"Zhang","first_name":"Wei"},{"first_name":"Ryuichi","last_name":"Shigemoto","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8761-9444","full_name":"Ryuichi Shigemoto"}],"type":"journal_article","extern":1,"publist_id":"4257","issue":"14","abstract":[{"text":"The Na+-K+ pump current (Ip) and the h-current (Ih) flowing through hyperpolarization-activated channels (h-channels) participate in generating the resting potential. These two currents are thought to be produced independently. We show here bidirectional interactions between Na+-K+ pumps and h-channels in mesencephalic trigeminal neurons. Activation of Ih leads to the generation of two types of ouabain-sensitive Ip with temporal profiles similar to those of instantaneous and slow components of I h, presumably reflecting Na+ transients in a restricted cellular space. Moreover, the Ip activated by instantaneous I h can facilitate the subsequent activation of slow Ih. Such counteractive and cooperative interactions were also disclosed by replacing extracellular Na+ with Li+, which is permeant through h-channels but does not stimulate the Na+-K+ pump as strongly as Na+ ions. These observations indicate that the interactions are bidirectional and mediated by Na+ ions. Also after substitution of extracellular Na+ with Li+, the tail Ih was reduced markedly despite an enhancement of Ih itself, attributable to a negative shift of the reversal potential for I h presumably caused by intracellular accumulation of Li+ ions. This suggests the presence of a microdomain where the interactions can take place. Thus, the bidirectional interactions between Na+-K + pumps and h-channels are likely to be mediated by Na+ microdomain. Consistent with these findings, hyperpolarization-activated and cyclic nucleotide-modulated subunits (HCN1/2) and the Na+-K + pump α3 isoform were colocalized in plasma membrane of mesencephalic trigeminal neurons having numerous spines.","lang":"eng"}],"page":"3694 - 3702","quality_controlled":0,"citation":{"short":"Y. Kang, T. Notomi, M. Saito, W. Zhang, R. Shigemoto, Journal of Neuroscience 24 (2004) 3694–3702.","mla":"Kang, Youngnam, et al. “Bidirectional Interactions between H-Channels and Na+-K + Pumps in Mesencephalic Trigeminal Neurons.” Journal of Neuroscience, vol. 24, no. 14, Society for Neuroscience, 2004, pp. 3694–702, doi:10.1523/JNEUROSCI.5641-03.2004.","chicago":"Kang, Youngnam, Takuya Notomi, Mitsuru Saito, Wei Zhang, and Ryuichi Shigemoto. “Bidirectional Interactions between H-Channels and Na+-K + Pumps in Mesencephalic Trigeminal Neurons.” Journal of Neuroscience. Society for Neuroscience, 2004. https://doi.org/10.1523/JNEUROSCI.5641-03.2004.","ama":"Kang Y, Notomi T, Saito M, Zhang W, Shigemoto R. Bidirectional interactions between H-channels and Na+-K + pumps in mesencephalic trigeminal neurons. Journal of Neuroscience. 2004;24(14):3694-3702. doi:10.1523/JNEUROSCI.5641-03.2004","ieee":"Y. Kang, T. Notomi, M. Saito, W. Zhang, and R. Shigemoto, “Bidirectional interactions between H-channels and Na+-K + pumps in mesencephalic trigeminal neurons,” Journal of Neuroscience, vol. 24, no. 14. Society for Neuroscience, pp. 3694–3702, 2004.","apa":"Kang, Y., Notomi, T., Saito, M., Zhang, W., & Shigemoto, R. (2004). Bidirectional interactions between H-channels and Na+-K + pumps in mesencephalic trigeminal neurons. Journal of Neuroscience. Society for Neuroscience. https://doi.org/10.1523/JNEUROSCI.5641-03.2004","ista":"Kang Y, Notomi T, Saito M, Zhang W, Shigemoto R. 2004. Bidirectional interactions between H-channels and Na+-K + pumps in mesencephalic trigeminal neurons. Journal of Neuroscience. 24(14), 3694–3702."},"publication":"Journal of Neuroscience","date_published":"2004-04-07T00:00:00Z","doi":"10.1523/JNEUROSCI.5641-03.2004","month":"04","day":"07"},{"type":"review","issue":"3 Suppl","publist_id":"4261","extern":1,"year":"2004","_id":"2636","publication_status":"published","status":"public","title":"Function and distribution of glutamate receptors in the central synapses","publisher":"Kyoritsu Shuppan","intvolume":" 49","author":[{"last_name":"Momiyama","first_name":"Akiko","full_name":"Momiyama, Akiko"},{"orcid":"0000-0001-8761-9444","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","last_name":"Shigemoto","first_name":"Ryuichi","full_name":"Ryuichi Shigemoto"}],"date_created":"2018-12-11T11:58:48Z","date_updated":"2020-07-14T12:45:44Z","volume":49,"month":"02","day":"01","publication":"Tanpakushitsu kakusan koso Protein nucleic acid enzyme","citation":{"mla":"Momiyama, Akiko, and Ryuichi Shigemoto. “Function and Distribution of Glutamate Receptors in the Central Synapses.” Tanpakushitsu Kakusan Koso Protein Nucleic Acid Enzyme, vol. 49, no. 3 Suppl, Kyoritsu Shuppan, 2004, pp. 287–94.","short":"A. Momiyama, R. Shigemoto, Tanpakushitsu Kakusan Koso Protein Nucleic Acid Enzyme 49 (2004) 287–294.","chicago":"Momiyama, Akiko, and Ryuichi Shigemoto. “Function and Distribution of Glutamate Receptors in the Central Synapses.” Tanpakushitsu Kakusan Koso Protein Nucleic Acid Enzyme. Kyoritsu Shuppan, 2004.","ama":"Momiyama A, Shigemoto R. Function and distribution of glutamate receptors in the central synapses. Tanpakushitsu kakusan koso Protein nucleic acid enzyme. 2004;49(3 Suppl):287-294.","ista":"Momiyama A, Shigemoto R. 2004. Function and distribution of glutamate receptors in the central synapses. Tanpakushitsu kakusan koso Protein nucleic acid enzyme. 49(3 Suppl), 287–294.","apa":"Momiyama, A., & Shigemoto, R. (2004). Function and distribution of glutamate receptors in the central synapses. Tanpakushitsu Kakusan Koso Protein Nucleic Acid Enzyme. Kyoritsu Shuppan.","ieee":"A. Momiyama and R. Shigemoto, “Function and distribution of glutamate receptors in the central synapses,” Tanpakushitsu kakusan koso Protein nucleic acid enzyme, vol. 49, no. 3 Suppl. Kyoritsu Shuppan, pp. 287–294, 2004."},"quality_controlled":0,"page":"287 - 294","date_published":"2004-02-01T00:00:00Z"}]