--- _id: '2628' abstract: - lang: eng text: We aimed to estimate the number of AMPA receptors (AMPARs) bound by the quantal transmitter packet, their single-channel conductance and their density in the postsynaptic membrane at cerebellar Purkinje cell synapses. The synaptic and extrasynaptic AMPARs were examined in Purkinje cells in 2- to 4-day-old rats, when they receive synaptic inputs solely from climbing fibres (CFs). Evoked CF EPSCs and whole-cell AMPA currents displayed roughly linear current-voltage relationships, consistent with the presence of GluR2 subunits in synaptic and extrasynaptic AMPARs. The mean quantal size, estimated from the miniature EPSCs (MEPSCs), was ∼300 pS. Peak-scaled non-stationary fluctuation analysis of spontaneous EPSCs and MEPSCs gave a weighted-mean synaptic channel conductance of ∼5 pS (∼7 pS when corrected for filtering). By applying non-stationary fluctuation analysis to extrasynaptic currents activated by brief glutamate pulses (5 mM), we also obtained a small single-channel conductance estimate for extrasynaptic AMPARs (∼11 pS). This approach allowed us to obtain a maximum open probability (Po,max) value for the extrasynaptic receptors (Po,max = 0.72). Directly resolved extrasynaptic channel openings in the continued presence of glutamate exhibited clear multiple-conductance levels. The mean area of the postsynaptic density (PSD) of these synapses was 0.074 μm2, measured by reconstructing electron-microscopic (EM) serial sections. Postembedding immunogold labelling by anti-GluR2/3 antibody revealed that AMPARs are localised in PSDs. From these data and by simulating error factors, we estimate that at least 66 AMPARs are bound by a quantal transmitter packet at CF-Purkinje cell synapses, and the receptors are packed at a minimum density of ∼900 μm-2 in the postsynaptic membrane. author: - first_name: Akiko full_name: Momiyama, Akiko last_name: Momiyama - first_name: Rachel full_name: Silver, Rachel A last_name: Silver - first_name: Michael full_name: Häusser, Michael A last_name: Häusser - first_name: Takuya full_name: Notomi, Takuya last_name: Notomi - first_name: Yue full_name: Wu, Yue last_name: Wu - first_name: Ryuichi full_name: Ryuichi Shigemoto id: 499F3ABC-F248-11E8-B48F-1D18A9856A87 last_name: Shigemoto orcid: 0000-0001-8761-9444 - first_name: Stuart full_name: Cull-Candy, Stuart G last_name: Cull Candy citation: ama: Momiyama A, Silver R, Häusser M, et al. The density of AMPA receptors activated by a transmitter quantum at the climbing fibre - Purkinje cell synapse in immature rats. Journal of Physiology. 2003;549(1):75-92. doi:10.1113/jphysiol.2002.033472 apa: Momiyama, A., Silver, R., Häusser, M., Notomi, T., Wu, Y., Shigemoto, R., & Cull Candy, S. (2003). The density of AMPA receptors activated by a transmitter quantum at the climbing fibre - Purkinje cell synapse in immature rats. Journal of Physiology. Wiley-Blackwell. https://doi.org/10.1113/jphysiol.2002.033472 chicago: Momiyama, Akiko, Rachel Silver, Michael Häusser, Takuya Notomi, Yue Wu, Ryuichi Shigemoto, and Stuart Cull Candy. “The Density of AMPA Receptors Activated by a Transmitter Quantum at the Climbing Fibre - Purkinje Cell Synapse in Immature Rats.” Journal of Physiology. Wiley-Blackwell, 2003. https://doi.org/10.1113/jphysiol.2002.033472. ieee: A. Momiyama et al., “The density of AMPA receptors activated by a transmitter quantum at the climbing fibre - Purkinje cell synapse in immature rats,” Journal of Physiology, vol. 549, no. 1. Wiley-Blackwell, pp. 75–92, 2003. ista: Momiyama A, Silver R, Häusser M, Notomi T, Wu Y, Shigemoto R, Cull Candy S. 2003. The density of AMPA receptors activated by a transmitter quantum at the climbing fibre - Purkinje cell synapse in immature rats. Journal of Physiology. 549(1), 75–92. mla: Momiyama, Akiko, et al. “The Density of AMPA Receptors Activated by a Transmitter Quantum at the Climbing Fibre - Purkinje Cell Synapse in Immature Rats.” Journal of Physiology, vol. 549, no. 1, Wiley-Blackwell, 2003, pp. 75–92, doi:10.1113/jphysiol.2002.033472. short: A. Momiyama, R. Silver, M. Häusser, T. Notomi, Y. Wu, R. Shigemoto, S. Cull Candy, Journal of Physiology 549 (2003) 75–92. date_created: 2018-12-11T11:58:45Z date_published: 2003-05-15T00:00:00Z date_updated: 2021-01-12T06:58:40Z day: '15' doi: 10.1113/jphysiol.2002.033472 extern: 1 intvolume: ' 549' issue: '1' month: '05' page: 75 - 92 publication: Journal of Physiology publication_status: published publisher: Wiley-Blackwell publist_id: '4270' quality_controlled: 0 status: public title: The density of AMPA receptors activated by a transmitter quantum at the climbing fibre - Purkinje cell synapse in immature rats type: journal_article volume: 549 year: '2003' ... --- _id: '2631' abstract: - lang: eng text: Cyclic ADP-ribose (cADP-ribose) is a putative second messenger or modulator. However, the role of cADP-ribose in the downstream signals of the metabotropic glutamate receptors (mGluRs) is unclear. Here, we show that glutamate stimulates ADP-ribosyl cyclase activity in rat or mouse crude membranes of retina via group III mGluRs or in superior cervical ganglion via group I mGluRs. The retina of mGluR6-deficient mice showed no increase in the ADP-ribosyl cyclase level in response to glutamate. GTP enhanced the initial rate of basal and glutamate-stimulated cyclase activity. GTP-γ-S also stimulated basal activity. To determine whether the coupling mode of mGluRs to ADP-ribosyl cyclase is a feature common to individual cloned mGluRs, we expressed each mGluR subtype in NG108-15 neuroblastoma x glioma hybrid cells. The glutamate-induced stimulation of the cyclase occurs preferentially in NG108-15 cells over-expressing mGluRs1, 3, 5, and 6. Cells expressing mGluR2 or mGluRs4 and 7 exhibit inhibition or no coupling, respectively. Glutamate-induced activation or inhibition of the cyclase activity was eliminated after pre-treatment with cholera or pertussis toxin, respectively. Thus, the subtype-specific coupling of mGluRs to ADP-ribosyl cyclase via G proteins suggests that some glutamate-evoked neuronal functions are mediated by cADP-ribose. author: - first_name: Haruhiro full_name: Higashida, Haruhiro last_name: Higashida - first_name: Jia full_name: Zhang, Jia-Sheng last_name: Zhang - first_name: Sumiko full_name: Mochida, Sumiko last_name: Mochida - first_name: Xiao full_name: Chen, Xiao-Liang last_name: Chen - first_name: Yeonsook full_name: Shin, Yeonsook last_name: Shin - first_name: Mami full_name: Noda, Mami last_name: Noda - first_name: Kazi full_name: Hossain, Kazi Z last_name: Hossain - first_name: Naoto full_name: Hoshi, Naoto last_name: Hoshi - first_name: Minako full_name: Hashii, Minako last_name: Hashii - first_name: Ryuichi full_name: Ryuichi Shigemoto id: 499F3ABC-F248-11E8-B48F-1D18A9856A87 last_name: Shigemoto orcid: 0000-0001-8761-9444 - first_name: Shigetada full_name: Nakanishi, Shigetada last_name: Nakanishi - first_name: Yutaka full_name: Fukuda, Yutaka last_name: Fukuda - first_name: Shigeru full_name: Yokoyama, Shigeru last_name: Yokoyama citation: ama: Higashida H, Zhang J, Mochida S, et al. Subtype-specific coupling with ADP-ribosyl cyclase of metabotropic glutamate receptors in retina, cervical superior ganglion and NG108-15 cells. Journal of Neurochemistry. 2003;85(5):1148-1158. doi:10.1046/j.1471-4159.2003.01751.x apa: Higashida, H., Zhang, J., Mochida, S., Chen, X., Shin, Y., Noda, M., … Yokoyama, S. (2003). Subtype-specific coupling with ADP-ribosyl cyclase of metabotropic glutamate receptors in retina, cervical superior ganglion and NG108-15 cells. Journal of Neurochemistry. Wiley-Blackwell. https://doi.org/10.1046/j.1471-4159.2003.01751.x chicago: Higashida, Haruhiro, Jia Zhang, Sumiko Mochida, Xiao Chen, Yeonsook Shin, Mami Noda, Kazi Hossain, et al. “Subtype-Specific Coupling with ADP-Ribosyl Cyclase of Metabotropic Glutamate Receptors in Retina, Cervical Superior Ganglion and NG108-15 Cells.” Journal of Neurochemistry. Wiley-Blackwell, 2003. https://doi.org/10.1046/j.1471-4159.2003.01751.x. ieee: H. Higashida et al., “Subtype-specific coupling with ADP-ribosyl cyclase of metabotropic glutamate receptors in retina, cervical superior ganglion and NG108-15 cells,” Journal of Neurochemistry, vol. 85, no. 5. Wiley-Blackwell, pp. 1148–1158, 2003. ista: Higashida H, Zhang J, Mochida S, Chen X, Shin Y, Noda M, Hossain K, Hoshi N, Hashii M, Shigemoto R, Nakanishi S, Fukuda Y, Yokoyama S. 2003. Subtype-specific coupling with ADP-ribosyl cyclase of metabotropic glutamate receptors in retina, cervical superior ganglion and NG108-15 cells. Journal of Neurochemistry. 85(5), 1148–1158. mla: Higashida, Haruhiro, et al. “Subtype-Specific Coupling with ADP-Ribosyl Cyclase of Metabotropic Glutamate Receptors in Retina, Cervical Superior Ganglion and NG108-15 Cells.” Journal of Neurochemistry, vol. 85, no. 5, Wiley-Blackwell, 2003, pp. 1148–58, doi:10.1046/j.1471-4159.2003.01751.x. short: H. Higashida, J. Zhang, S. Mochida, X. Chen, Y. Shin, M. Noda, K. Hossain, N. Hoshi, M. Hashii, R. Shigemoto, S. Nakanishi, Y. Fukuda, S. Yokoyama, Journal of Neurochemistry 85 (2003) 1148–1158. date_created: 2018-12-11T11:58:46Z date_published: 2003-06-01T00:00:00Z date_updated: 2021-01-12T06:58:42Z day: '01' doi: 10.1046/j.1471-4159.2003.01751.x extern: 1 intvolume: ' 85' issue: '5' month: '06' page: 1148 - 1158 publication: Journal of Neurochemistry publication_status: published publisher: Wiley-Blackwell publist_id: '4268' quality_controlled: 0 status: public title: Subtype-specific coupling with ADP-ribosyl cyclase of metabotropic glutamate receptors in retina, cervical superior ganglion and NG108-15 cells type: journal_article volume: 85 year: '2003' ... --- _id: '2633' abstract: - lang: eng text: The modulation of calcium channels by metabotropic glutamate receptors (mGluRs) is a key event in the fine-tuning of neurotransmitter release. Here we report that, in cerebrocortical nerve terminals of adult rats, the inhibition of glutamate release is mediated by mGluR7. In this preparation, the major component of glutamate release is supported by P/Q-type Ca2+ channels (72.7%). However, mGluR7 selectively reduced the release component that is associated with N-type Ca2+ channels (29.9%). Inhibition of P/Q channels by mGluR7 is not masked by the higher efficiency of these channels in driving glutamate release when compared with N-type channels. Thus, activation of mGluR7 failed to reduce the release associated with P/Q channels when the extracellular calcium concentration, ([Ca2+]o), was reduced from 1.3 to 0.5 mM. Through Ca2+ imaging, we show that Ca2+ channels are distributed in a heterogeneous manner in individual nerve terminals. Indeed, in this preparation, nerve terminals were observed that contain N-type (31.1%; conotoxin GVIA-sensitive) or P/Q-type (64.3%; agatoxin IVA-sensitive) channels or that were insensitive to these two toxins (4.6%). Interestingly, the great majority of the responses to L-AP4 (95.4%) were observed in nerve terminals containing N-type channels. This specific co-localization of mGluR7 and N-type Ca2+-channels could explain the failure of the receptor to inhibit the P/Q channel-associated release component and also reveal the existence of specific targeting mechanisms to localize the two proteins in the same nerve terminal subset. author: - first_name: Carmelo full_name: Millán, Carmelo last_name: Millán - first_name: Enrique full_name: Castro, Enrique G last_name: Castro - first_name: Magdalena full_name: Torres, Magdalena last_name: Torres - first_name: Ryuichi full_name: Ryuichi Shigemoto id: 499F3ABC-F248-11E8-B48F-1D18A9856A87 last_name: Shigemoto orcid: 0000-0001-8761-9444 - first_name: José full_name: Sánchez-Prieto, José last_name: Sánchez Prieto citation: ama: Millán C, Castro E, Torres M, Shigemoto R, Sánchez Prieto J. Co-expression of metabotropic glutamate receptor 7 and N-type Ca2+ channels in single cerebrocortical nerve terminals of adult rats. Journal of Biological Chemistry. 2003;278(26):23955-23962. doi:10.1074/jbc.M211471200 apa: Millán, C., Castro, E., Torres, M., Shigemoto, R., & Sánchez Prieto, J. (2003). Co-expression of metabotropic glutamate receptor 7 and N-type Ca2+ channels in single cerebrocortical nerve terminals of adult rats. Journal of Biological Chemistry. American Society for Biochemistry and Molecular Biology. https://doi.org/10.1074/jbc.M211471200 chicago: Millán, Carmelo, Enrique Castro, Magdalena Torres, Ryuichi Shigemoto, and José Sánchez Prieto. “Co-Expression of Metabotropic Glutamate Receptor 7 and N-Type Ca2+ Channels in Single Cerebrocortical Nerve Terminals of Adult Rats.” Journal of Biological Chemistry. American Society for Biochemistry and Molecular Biology, 2003. https://doi.org/10.1074/jbc.M211471200. ieee: C. Millán, E. Castro, M. Torres, R. Shigemoto, and J. Sánchez Prieto, “Co-expression of metabotropic glutamate receptor 7 and N-type Ca2+ channels in single cerebrocortical nerve terminals of adult rats,” Journal of Biological Chemistry, vol. 278, no. 26. American Society for Biochemistry and Molecular Biology, pp. 23955–23962, 2003. ista: Millán C, Castro E, Torres M, Shigemoto R, Sánchez Prieto J. 2003. Co-expression of metabotropic glutamate receptor 7 and N-type Ca2+ channels in single cerebrocortical nerve terminals of adult rats. Journal of Biological Chemistry. 278(26), 23955–23962. mla: Millán, Carmelo, et al. “Co-Expression of Metabotropic Glutamate Receptor 7 and N-Type Ca2+ Channels in Single Cerebrocortical Nerve Terminals of Adult Rats.” Journal of Biological Chemistry, vol. 278, no. 26, American Society for Biochemistry and Molecular Biology, 2003, pp. 23955–62, doi:10.1074/jbc.M211471200. short: C. Millán, E. Castro, M. Torres, R. Shigemoto, J. Sánchez Prieto, Journal of Biological Chemistry 278 (2003) 23955–23962. date_created: 2018-12-11T11:58:47Z date_published: 2003-07-27T00:00:00Z date_updated: 2021-01-12T06:58:42Z day: '27' doi: 10.1074/jbc.M211471200 extern: 1 intvolume: ' 278' issue: '26' month: '07' page: 23955 - 23962 publication: Journal of Biological Chemistry publication_status: published publisher: American Society for Biochemistry and Molecular Biology publist_id: '4265' quality_controlled: 0 status: public title: Co-expression of metabotropic glutamate receptor 7 and N-type Ca2+ channels in single cerebrocortical nerve terminals of adult rats type: journal_article volume: 278 year: '2003' ... --- _id: '2632' abstract: - lang: eng text: In many brain regions, hyperpolarization-activated cationic currents (Ih) are involved in the generation of rhythmic activities, but the role of Ih in olfactory oscillations remains unclear. Knowledge of the cellular and subcellular distributions of hyperpolarization-activated and cyclic nucleotide-gated channel (HCN) subunits is necessary for understanding the role of Ih in olfactory network activities. Using light microscopic immunocytochemistry, we demonstrate strong HCN1 labelling of the glomerular layer and moderate staining of granule cell, internal and external plexiform layers of the rat main olfactory bulb. In the glomerular layer, among many unlabelled neurons, two distinct subpopulations of juxtaglomerular cells are labelled. Approximately 10% of the juxtaglomerular cells strongly express HCN1. These small diameter cells are immunoreactive for GABA and comprise a subpopulation of periglomerular cells. An additional subset of juxtaglomerular cells (≈ 1%) expresses low levels of HCN1. They are large in diameter, GABA immunonegative but immunopositive for vesicular glutamate transporter 2, characterizing them as external tufted cells. Quantitative immunogold localization revealed that the somatic plasma membranes of periglomerular cells contain approximately four times more HCN1 labelling than those of external tufted cells. Unlike in cortical pyramidal cells, immunogold density for HCN1 does not significantly differ in somatic and dendritic plasma membranes of external tufted cells, indicating that post-synaptic potentials arriving at proximal and distal dendrites are modulated by the same density of I h. Our results demonstrate a cell type-dependent expression of HCN1 in the olfactory bulb and predict a differential contribution of distinct juxtaglomerular cell types to network oscillations. author: - first_name: Noémi full_name: Holderith, Noémi B last_name: Holderith - first_name: Ryuichi full_name: Ryuichi Shigemoto id: 499F3ABC-F248-11E8-B48F-1D18A9856A87 last_name: Shigemoto orcid: 0000-0001-8761-9444 - first_name: Zoltán full_name: Nusser, Zoltán last_name: Nusser citation: ama: Holderith N, Shigemoto R, Nusser Z. Cell type-dependent expression of HCN1 in the main olfactory bulb. European Journal of Neuroscience. 2003;18(2):344-354. doi:10.1046/j.1460-9568.2003.02756.x apa: Holderith, N., Shigemoto, R., & Nusser, Z. (2003). Cell type-dependent expression of HCN1 in the main olfactory bulb. European Journal of Neuroscience. Wiley-Blackwell. https://doi.org/10.1046/j.1460-9568.2003.02756.x chicago: Holderith, Noémi, Ryuichi Shigemoto, and Zoltán Nusser. “Cell Type-Dependent Expression of HCN1 in the Main Olfactory Bulb.” European Journal of Neuroscience. Wiley-Blackwell, 2003. https://doi.org/10.1046/j.1460-9568.2003.02756.x. ieee: N. Holderith, R. Shigemoto, and Z. Nusser, “Cell type-dependent expression of HCN1 in the main olfactory bulb,” European Journal of Neuroscience, vol. 18, no. 2. Wiley-Blackwell, pp. 344–354, 2003. ista: Holderith N, Shigemoto R, Nusser Z. 2003. Cell type-dependent expression of HCN1 in the main olfactory bulb. European Journal of Neuroscience. 18(2), 344–354. mla: Holderith, Noémi, et al. “Cell Type-Dependent Expression of HCN1 in the Main Olfactory Bulb.” European Journal of Neuroscience, vol. 18, no. 2, Wiley-Blackwell, 2003, pp. 344–54, doi:10.1046/j.1460-9568.2003.02756.x. short: N. Holderith, R. Shigemoto, Z. Nusser, European Journal of Neuroscience 18 (2003) 344–354. date_created: 2018-12-11T11:58:47Z date_published: 2003-07-01T00:00:00Z date_updated: 2021-01-12T06:58:42Z day: '01' doi: 10.1046/j.1460-9568.2003.02756.x extern: 1 intvolume: ' 18' issue: '2' month: '07' page: 344 - 354 publication: European Journal of Neuroscience publication_status: published publisher: Wiley-Blackwell publist_id: '4266' quality_controlled: 0 status: public title: Cell type-dependent expression of HCN1 in the main olfactory bulb type: journal_article volume: 18 year: '2003' ... --- _id: '2635' abstract: - lang: eng text: Metabotropic GABAB receptors mediate slow inhibitory effects presynaptically and postsynaptically. Using preembedding immunohistochemical methods combined with quantitative analysis of GABAB receptor subunit immunoreactivity, this study provides a detailed description of the cellular and subcellular localization of GABAB1a/b and GABA B2 in the rat hippocampus. At the light microscopic level, an overlapping distribution of GABAB1a/b and GABAB2 was revealed in the dendritic layers of the hippocampus. In addition, expression of the GABAB1a/b subunit was found in somata of CA1 pyramidal cells and of a subset of GABAergic interneurons. At the electron microscopic level, immunoreactivity for both subunits was observed on presynaptic and, more abundantly, on postsynaptic elements. Presynaptically, subunits were mainly detected in the extrasynaptic membrane and occasionally over the presynaptic membrane specialization of putative glutamatergic and, to a lesser extent, GABAergic axon terminals. Postsynaptically, the majority of GABAB receptor subunits were localized to the extrasynaptic plasma membrane of spines and dendritic shafts of principal cells and shafts of interneuron dendrites. Quantitative analysis revealed enrichment of GABAB1a/b around putative glutamatergic synapses on spines and an even distribution on dendritic shafts of pyramidal cells contacted by GABAergic boutons. The association of GABAB receptors with glutamatergic synapses at both presynaptic and postsynaptic sides indicates their intimate involvement in the modulation of glutamatergic neurotransmission. The dominant extrasynaptic localization of GABAB receptor subunits suggests that their activation is dependent on spillover of GABA requiring simultaneous activity of populations of GABAergic cells as it occurs during population oscillations or epileptic seizures. author: - first_name: Ákos full_name: Kulik, Ákos last_name: Kulik - first_name: Imre full_name: Vida, Imre last_name: Vida - first_name: Rafael full_name: Luján, Rafael last_name: Luján - first_name: Carola full_name: Haas, Carola A last_name: Haas - first_name: Guillermina full_name: López-Bendito, Guillermina last_name: López Bendito - first_name: Ryuichi full_name: Ryuichi Shigemoto id: 499F3ABC-F248-11E8-B48F-1D18A9856A87 last_name: Shigemoto orcid: 0000-0001-8761-9444 - first_name: Michael full_name: Frotscher, Michael last_name: Frotscher citation: ama: Kulik Á, Vida I, Luján R, et al. Subcellular Localization of Metabotropic GABAB Receptor Subunits GABAB1a/b and GABAB2 in the Rat Hippocampus. Journal of Neuroscience. 2003;23(35):11026-11035. apa: Kulik, Á., Vida, I., Luján, R., Haas, C., López Bendito, G., Shigemoto, R., & Frotscher, M. (2003). Subcellular Localization of Metabotropic GABAB Receptor Subunits GABAB1a/b and GABAB2 in the Rat Hippocampus. Journal of Neuroscience. Society for Neuroscience. chicago: Kulik, Ákos, Imre Vida, Rafael Luján, Carola Haas, Guillermina López Bendito, Ryuichi Shigemoto, and Michael Frotscher. “Subcellular Localization of Metabotropic GABAB Receptor Subunits GABAB1a/b and GABAB2 in the Rat Hippocampus.” Journal of Neuroscience. Society for Neuroscience, 2003. ieee: Á. Kulik et al., “Subcellular Localization of Metabotropic GABAB Receptor Subunits GABAB1a/b and GABAB2 in the Rat Hippocampus,” Journal of Neuroscience, vol. 23, no. 35. Society for Neuroscience, pp. 11026–11035, 2003. ista: Kulik Á, Vida I, Luján R, Haas C, López Bendito G, Shigemoto R, Frotscher M. 2003. Subcellular Localization of Metabotropic GABAB Receptor Subunits GABAB1a/b and GABAB2 in the Rat Hippocampus. Journal of Neuroscience. 23(35), 11026–11035. mla: Kulik, Ákos, et al. “Subcellular Localization of Metabotropic GABAB Receptor Subunits GABAB1a/b and GABAB2 in the Rat Hippocampus.” Journal of Neuroscience, vol. 23, no. 35, Society for Neuroscience, 2003, pp. 11026–35. short: Á. Kulik, I. Vida, R. Luján, C. Haas, G. López Bendito, R. Shigemoto, M. Frotscher, Journal of Neuroscience 23 (2003) 11026–11035. date_created: 2018-12-11T11:58:47Z date_published: 2003-12-03T00:00:00Z date_updated: 2021-01-12T06:58:43Z day: '03' extern: 1 intvolume: ' 23' issue: '35' month: '12' page: 11026 - 11035 publication: Journal of Neuroscience publication_status: published publisher: Society for Neuroscience publist_id: '4263' quality_controlled: 0 status: public title: Subcellular Localization of Metabotropic GABAB Receptor Subunits GABAB1a/b and GABAB2 in the Rat Hippocampus type: journal_article volume: 23 year: '2003' ... --- _id: '2634' abstract: - lang: eng text: To better understand the role of neurotransmitter receptors in neuronal differentiation and maturation a detailed knowledge of their identity, location and function in the plasma membrane of specific neuronal populations during development is required. Combining pre-embedding immunocytochemistry with cell tracking in embryonic brain slice cultures we show that virtually all neurons (∼98%) migrating through the lower intermediate zone (LIZ) on their way from the medial ganglionic eminence to the cerebral cortex, express GABA BR1. Blockade of GABABRs with a specific antagonist, CGP52432, resulted in a concentration-dependent accumulation of these tangentially migrating neurons in the ventricular/subventricular zones (VZ/SVZ) of the cortex and fewer cells were observed in the cortical plate/marginal zone (CP/MZ) and LIZ. Moreover, they had significantly shorter leading processes compared with similar migrating cells in control slices. Electrophysiological recording in LIZ and CP cells revealed no direct effect of either CGP52432 or the GABABR agonist, baclofen, on resting membrane properties suggesting that the effect of CGP52432 on migration might be mediated through a metabotropic action or the regulation of release of factors controlling migration. These results suggest that GABABRs have an important modulatory role in the migration of cortical interneurons. author: - first_name: Guillermina full_name: López-Bendito, Guillermina last_name: López Bendito - first_name: Rafael full_name: Luján, Rafael last_name: Luján - first_name: Ryuichi full_name: Ryuichi Shigemoto id: 499F3ABC-F248-11E8-B48F-1D18A9856A87 last_name: Shigemoto orcid: 0000-0001-8761-9444 - first_name: Paul full_name: Ganter, Paul last_name: Ganter - first_name: Ole full_name: Paulsen, Ole last_name: Paulsen - first_name: Zoltán full_name: Molnár, Zoltán last_name: Molnár citation: ama: López Bendito G, Luján R, Shigemoto R, Ganter P, Paulsen O, Molnár Z. Blockade of GABAB receptors alters the tangential migration of cortical neurons. Cerebral Cortex. 2003;13(9):932-942. doi:10.1093/cercor/13.9.932 apa: López Bendito, G., Luján, R., Shigemoto, R., Ganter, P., Paulsen, O., & Molnár, Z. (2003). Blockade of GABAB receptors alters the tangential migration of cortical neurons. Cerebral Cortex. Oxford University Press. https://doi.org/10.1093/cercor/13.9.932 chicago: López Bendito, Guillermina, Rafael Luján, Ryuichi Shigemoto, Paul Ganter, Ole Paulsen, and Zoltán Molnár. “Blockade of GABAB Receptors Alters the Tangential Migration of Cortical Neurons.” Cerebral Cortex. Oxford University Press, 2003. https://doi.org/10.1093/cercor/13.9.932. ieee: G. López Bendito, R. Luján, R. Shigemoto, P. Ganter, O. Paulsen, and Z. Molnár, “Blockade of GABAB receptors alters the tangential migration of cortical neurons,” Cerebral Cortex, vol. 13, no. 9. Oxford University Press, pp. 932–942, 2003. ista: López Bendito G, Luján R, Shigemoto R, Ganter P, Paulsen O, Molnár Z. 2003. Blockade of GABAB receptors alters the tangential migration of cortical neurons. Cerebral Cortex. 13(9), 932–942. mla: López Bendito, Guillermina, et al. “Blockade of GABAB Receptors Alters the Tangential Migration of Cortical Neurons.” Cerebral Cortex, vol. 13, no. 9, Oxford University Press, 2003, pp. 932–42, doi:10.1093/cercor/13.9.932. short: G. López Bendito, R. Luján, R. Shigemoto, P. Ganter, O. Paulsen, Z. Molnár, Cerebral Cortex 13 (2003) 932–942. date_created: 2018-12-11T11:58:47Z date_published: 2003-09-01T00:00:00Z date_updated: 2021-01-12T06:58:43Z day: '01' doi: 10.1093/cercor/13.9.932 extern: 1 intvolume: ' 13' issue: '9' month: '09' page: 932 - 942 publication: Cerebral Cortex publication_status: published publisher: Oxford University Press publist_id: '4264' quality_controlled: 0 status: public title: Blockade of GABAB receptors alters the tangential migration of cortical neurons type: journal_article volume: 13 year: '2003' ... --- _id: '2630' abstract: - lang: eng text: Taste-metabotropic glutamate receptor 4 (taste-mGluR4) and the heteromers of T1R1 and T1R3 are candidate receptors involved in the sense of umami (monosodium glutamate) taste. Although the expression of group III mGluRs (taste-mGluR4) has been demonstrated in taste tissues, no mention has been made of the expression of group I mGluRs (mGluR1 and mGluR5) in taste tissues. We examined the expression of mGluR1 and mGluR5 in rat gustatory tissues by using reverse transcription-polymerase chain reaction (RT-PCR), in situ hybridization, immunohistochemistry and immunoelectron microscopy. RT-PCR assay showed that mGluR1α and mGluR1β mRNAs were expressed in circumvallate papillae, but mGluR5 mRNA was not expressed. The positive signals of mGluR1 mRNA were detected only in circumvallate taste buds by in situ hybridization analysis. In cryosections of fungiform, foliate and circumvallate papillae, the antibody against mGluRla gave intense labeling on the taste hairs in all taste pores examined. In the developing taste buds, the positive signals of mGluR1α in taste hairs gradually increased with the increase in number of taste bud cells. These results show that, in addition to taste-mGluR4 and the heteromer of T1R1 and T1R3, mGluR1α may function as a receptor for glutamate (umami) taste sensation. author: - first_name: Takashi full_name: Toyono, Takashi last_name: Toyono - first_name: Yuji full_name: Seta, Yuji last_name: Seta - first_name: Shinji full_name: Kataoka, Shinji last_name: Kataoka - first_name: Shintaro full_name: Kawano, Shintaro last_name: Kawano - first_name: Ryuichi full_name: Ryuichi Shigemoto id: 499F3ABC-F248-11E8-B48F-1D18A9856A87 last_name: Shigemoto orcid: 0000-0001-8761-9444 - first_name: Kuniaki full_name: Toyoshima, Kuniaki last_name: Toyoshima citation: ama: Toyono T, Seta Y, Kataoka S, Kawano S, Shigemoto R, Toyoshima K. Expression of metabotropic glutamate receptor group I in rat gustatory papillae. Cell and Tissue Research. 2003;313(1):29-35. doi:10.1007/s00441-003-0740-2 apa: Toyono, T., Seta, Y., Kataoka, S., Kawano, S., Shigemoto, R., & Toyoshima, K. (2003). Expression of metabotropic glutamate receptor group I in rat gustatory papillae. Cell and Tissue Research. Springer. https://doi.org/10.1007/s00441-003-0740-2 chicago: Toyono, Takashi, Yuji Seta, Shinji Kataoka, Shintaro Kawano, Ryuichi Shigemoto, and Kuniaki Toyoshima. “Expression of Metabotropic Glutamate Receptor Group I in Rat Gustatory Papillae.” Cell and Tissue Research. Springer, 2003. https://doi.org/10.1007/s00441-003-0740-2. ieee: T. Toyono, Y. Seta, S. Kataoka, S. Kawano, R. Shigemoto, and K. Toyoshima, “Expression of metabotropic glutamate receptor group I in rat gustatory papillae,” Cell and Tissue Research, vol. 313, no. 1. Springer, pp. 29–35, 2003. ista: Toyono T, Seta Y, Kataoka S, Kawano S, Shigemoto R, Toyoshima K. 2003. Expression of metabotropic glutamate receptor group I in rat gustatory papillae. Cell and Tissue Research. 313(1), 29–35. mla: Toyono, Takashi, et al. “Expression of Metabotropic Glutamate Receptor Group I in Rat Gustatory Papillae.” Cell and Tissue Research, vol. 313, no. 1, Springer, 2003, pp. 29–35, doi:10.1007/s00441-003-0740-2. short: T. Toyono, Y. Seta, S. Kataoka, S. Kawano, R. Shigemoto, K. Toyoshima, Cell and Tissue Research 313 (2003) 29–35. date_created: 2018-12-11T11:58:46Z date_published: 2003-07-01T00:00:00Z date_updated: 2021-01-12T06:58:41Z day: '01' doi: 10.1007/s00441-003-0740-2 extern: 1 intvolume: ' 313' issue: '1' month: '07' page: 29 - 35 publication: Cell and Tissue Research publication_status: published publisher: Springer publist_id: '4267' quality_controlled: 0 status: public title: Expression of metabotropic glutamate receptor group I in rat gustatory papillae type: journal_article volume: 313 year: '2003' ... --- _id: '2637' abstract: - lang: eng text: While the cholinergic depletion in Alzheimer's disease (AD) has been known for some time, a definitive involvement of other neurotransmitter systems has been somewhat more elusive. Our study demonstrates a clear involvement of both glutamatergic and, to a lesser extent, GABAergic neurons in an early onset transgenic mouse model of AD-like amyloid pathology. Immunohistochemical staining and subsequent quantification has revealed a statistically significant increased density of glutamatergic and GABAergic presynaptic boutons in both the plaque free and plaque adjacent cortical neuropile areas of transgenic mice as compared to non-transgenic controls. Furthermore, amyloid plaque size was shown to have a statistically significant effect on the relative area occupied by dystrophic glutamatergic neurites in the peri-plaque neuropile. These findings support our hypothesis that the amyloid pathology progresses in a time and neurotransmitter specific manner, first in the cholinergic system which appears to be most vulnerable, followed by the glutamatergic presynaptic boutons and finally the somewhat more resilient GABAergic terminals. author: - first_name: Karen full_name: Bell, Karen F last_name: Bell - first_name: G J full_name: De Kort, G J last_name: De Kort - first_name: S full_name: Steggerda, S last_name: Steggerda - first_name: Ryuichi full_name: Ryuichi Shigemoto id: 499F3ABC-F248-11E8-B48F-1D18A9856A87 last_name: Shigemoto orcid: 0000-0001-8761-9444 - first_name: Alfredo full_name: Ribeiro-da-Silva, Alfredo last_name: Ribeiro Da Silva - first_name: Augusto full_name: Cuello, Augusto C last_name: Cuello citation: ama: Bell K, De Kort GJ, Steggerda S, Shigemoto R, Ribeiro Da Silva A, Cuello A. Structural involvement of the glutamatergic presynaptic boutons in a transgenic mouse model expressing early onset amyloid pathology. Neuroscience Letters. 2003;353(2):143-147. doi:10.1016/j.neulet.2003.09.027 apa: Bell, K., De Kort, G. J., Steggerda, S., Shigemoto, R., Ribeiro Da Silva, A., & Cuello, A. (2003). Structural involvement of the glutamatergic presynaptic boutons in a transgenic mouse model expressing early onset amyloid pathology. Neuroscience Letters. Elsevier. https://doi.org/10.1016/j.neulet.2003.09.027 chicago: Bell, Karen, G J De Kort, S Steggerda, Ryuichi Shigemoto, Alfredo Ribeiro Da Silva, and Augusto Cuello. “Structural Involvement of the Glutamatergic Presynaptic Boutons in a Transgenic Mouse Model Expressing Early Onset Amyloid Pathology.” Neuroscience Letters. Elsevier, 2003. https://doi.org/10.1016/j.neulet.2003.09.027. ieee: K. Bell, G. J. De Kort, S. Steggerda, R. Shigemoto, A. Ribeiro Da Silva, and A. Cuello, “Structural involvement of the glutamatergic presynaptic boutons in a transgenic mouse model expressing early onset amyloid pathology,” Neuroscience Letters, vol. 353, no. 2. Elsevier, pp. 143–147, 2003. ista: Bell K, De Kort GJ, Steggerda S, Shigemoto R, Ribeiro Da Silva A, Cuello A. 2003. Structural involvement of the glutamatergic presynaptic boutons in a transgenic mouse model expressing early onset amyloid pathology. Neuroscience Letters. 353(2), 143–147. mla: Bell, Karen, et al. “Structural Involvement of the Glutamatergic Presynaptic Boutons in a Transgenic Mouse Model Expressing Early Onset Amyloid Pathology.” Neuroscience Letters, vol. 353, no. 2, Elsevier, 2003, pp. 143–47, doi:10.1016/j.neulet.2003.09.027. short: K. Bell, G.J. De Kort, S. Steggerda, R. Shigemoto, A. Ribeiro Da Silva, A. Cuello, Neuroscience Letters 353 (2003) 143–147. date_created: 2018-12-11T11:58:48Z date_published: 2003-12-19T00:00:00Z date_updated: 2021-01-12T06:58:44Z day: '19' doi: 10.1016/j.neulet.2003.09.027 extern: 1 intvolume: ' 353' issue: '2' month: '12' page: 143 - 147 publication: Neuroscience Letters publication_status: published publisher: Elsevier publist_id: '4262' quality_controlled: 0 status: public title: Structural involvement of the glutamatergic presynaptic boutons in a transgenic mouse model expressing early onset amyloid pathology type: journal_article volume: 353 year: '2003' ... --- _id: '2784' abstract: - lang: eng text: We report the results of an experimental study of magnetohydrodynamic damping of sidewall convection in a rectangular enclosure filled with gallium. In particular we investigate the suppression of convection when a steady magnetic field is applied separately in each of the three principal directions of the flow. The strongest damping of the steady flow is found for a vertical magnetic field, which is in agreement with theory. However, we observe that the application of a field transverse to the flow provides greater damping than a longitudinal one, which seems to contradict available theory. We provide a possible resolution of this apparent dichotomy in terms of the length scale of the experiment. author: - first_name: Björn full_name: Björn Hof id: 3A374330-F248-11E8-B48F-1D18A9856A87 last_name: Hof orcid: 0000-0003-2057-2754 - first_name: Anne full_name: Juel, Anne last_name: Juel - first_name: Tom full_name: Mullin, Tom P last_name: Mullin citation: ama: Hof B, Juel A, Mullin T. Magnetohydrodynamic damping of convective flows in molten gallium. Journal of Fluid Mechanics. 2003;482:163-179. doi:10.1017/S0022112003004014 apa: Hof, B., Juel, A., & Mullin, T. (2003). Magnetohydrodynamic damping of convective flows in molten gallium. Journal of Fluid Mechanics. Cambridge University Press. https://doi.org/10.1017/S0022112003004014 chicago: Hof, Björn, Anne Juel, and Tom Mullin. “Magnetohydrodynamic Damping of Convective Flows in Molten Gallium.” Journal of Fluid Mechanics. Cambridge University Press, 2003. https://doi.org/10.1017/S0022112003004014. ieee: B. Hof, A. Juel, and T. Mullin, “Magnetohydrodynamic damping of convective flows in molten gallium,” Journal of Fluid Mechanics, vol. 482. Cambridge University Press, pp. 163–179, 2003. ista: Hof B, Juel A, Mullin T. 2003. Magnetohydrodynamic damping of convective flows in molten gallium. Journal of Fluid Mechanics. 482, 163–179. mla: Hof, Björn, et al. “Magnetohydrodynamic Damping of Convective Flows in Molten Gallium.” Journal of Fluid Mechanics, vol. 482, Cambridge University Press, 2003, pp. 163–79, doi:10.1017/S0022112003004014. short: B. Hof, A. Juel, T. Mullin, Journal of Fluid Mechanics 482 (2003) 163–179. date_created: 2018-12-11T11:59:35Z date_published: 2003-05-13T00:00:00Z date_updated: 2021-01-12T06:59:42Z day: '13' doi: 10.1017/S0022112003004014 extern: 1 intvolume: ' 482' month: '05' page: 163 - 179 publication: Journal of Fluid Mechanics publication_status: published publisher: Cambridge University Press publist_id: '4105' quality_controlled: 0 status: public title: Magnetohydrodynamic damping of convective flows in molten gallium type: journal_article volume: 482 year: '2003' ... --- _id: '2785' abstract: - lang: eng text: Experimental evidence for the scaling of the finite amplitude of perturbation theory required to promote transition in Poiseuille flow was found. The exponent is -1 and was uncovered using considerable care in the design and execution of the experiment. Interestingly, this exponent was also found in experiments on transition in boundary layers. author: - first_name: Björn full_name: Björn Hof id: 3A374330-F248-11E8-B48F-1D18A9856A87 last_name: Hof orcid: 0000-0003-2057-2754 - first_name: Anne full_name: Juel, Anne last_name: Juel - first_name: Tom full_name: Mullin, Tom P last_name: Mullin citation: ama: Hof B, Juel A, Mullin T. Scaling of the turbulence transition threshold in a pipe. Physical Review Letters. 2003;91(24):244502/1-244502/4. doi:10.1103/PhysRevLett.91.244502 apa: Hof, B., Juel, A., & Mullin, T. (2003). Scaling of the turbulence transition threshold in a pipe. Physical Review Letters. American Physical Society. https://doi.org/10.1103/PhysRevLett.91.244502 chicago: Hof, Björn, Anne Juel, and Tom Mullin. “Scaling of the Turbulence Transition Threshold in a Pipe.” Physical Review Letters. American Physical Society, 2003. https://doi.org/10.1103/PhysRevLett.91.244502. ieee: B. Hof, A. Juel, and T. Mullin, “Scaling of the turbulence transition threshold in a pipe,” Physical Review Letters, vol. 91, no. 24. American Physical Society, p. 244502/1-244502/4, 2003. ista: Hof B, Juel A, Mullin T. 2003. Scaling of the turbulence transition threshold in a pipe. Physical Review Letters. 91(24), 244502/1-244502/4. mla: Hof, Björn, et al. “Scaling of the Turbulence Transition Threshold in a Pipe.” Physical Review Letters, vol. 91, no. 24, American Physical Society, 2003, p. 244502/1-244502/4, doi:10.1103/PhysRevLett.91.244502. short: B. Hof, A. Juel, T. Mullin, Physical Review Letters 91 (2003) 244502/1-244502/4. date_created: 2018-12-11T11:59:35Z date_published: 2003-12-12T00:00:00Z date_updated: 2021-01-12T06:59:42Z day: '12' doi: 10.1103/PhysRevLett.91.244502 extern: 1 intvolume: ' 91' issue: '24' month: '12' page: 244502/1 - 244502/4 publication: Physical Review Letters publication_status: published publisher: American Physical Society publist_id: '4104' quality_controlled: 0 status: public title: Scaling of the turbulence transition threshold in a pipe type: journal_article volume: 91 year: '2003' ...