[{"title":"Toric hyperkähler varieties","article_processing_charge":"No","external_id":{"arxiv":["math/0203096"]},"publist_id":"5741","author":[{"last_name":"Hausel","full_name":"Hausel, Tamas","first_name":"Tamas","id":"4A0666D8-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Bernd","last_name":"Sturmfels","full_name":"Sturmfels, Bernd"}],"user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","citation":{"chicago":"Hausel, Tamás, and Bernd Sturmfels. “Toric Hyperkähler Varieties.” Documenta Mathematica. Deutsche Mathematiker Vereinigung, 2002. https://doi.org/10.4171/DM/130.","ista":"Hausel T, Sturmfels B. 2002. Toric hyperkähler varieties. Documenta Mathematica. 7(1), 495–534.","mla":"Hausel, Tamás, and Bernd Sturmfels. “Toric Hyperkähler Varieties.” Documenta Mathematica, vol. 7, no. 1, Deutsche Mathematiker Vereinigung, 2002, pp. 495–534, doi:10.4171/DM/130.","apa":"Hausel, T., & Sturmfels, B. (2002). Toric hyperkähler varieties. Documenta Mathematica. Deutsche Mathematiker Vereinigung. https://doi.org/10.4171/DM/130","ama":"Hausel T, Sturmfels B. Toric hyperkähler varieties. Documenta Mathematica. 2002;7(1):495-534. doi:10.4171/DM/130","ieee":"T. Hausel and B. Sturmfels, “Toric hyperkähler varieties,” Documenta Mathematica, vol. 7, no. 1. Deutsche Mathematiker Vereinigung, pp. 495–534, 2002.","short":"T. Hausel, B. Sturmfels, Documenta Mathematica 7 (2002) 495–534."},"date_created":"2018-12-11T11:52:06Z","doi":"10.4171/DM/130","date_published":"2002-01-01T00:00:00Z","page":"495 - 534","publication":"Documenta Mathematica","day":"01","year":"2002","oa":1,"quality_controlled":"1","publisher":"Deutsche Mathematiker Vereinigung","acknowledgement":"Both authors were supported by the Miller Institute for Basic Research in Science, in the form of a Miller Research Fellowship (1999-2002) for the first author and a Miller Professorship (2000-2001) for the second author. The second author was also supported by the National Science\r\nFoundation (DMS-9970254).","extern":"1","date_updated":"2023-07-26T09:16:33Z","status":"public","type":"journal_article","article_type":"original","_id":"1451","volume":7,"issue":"1","language":[{"iso":"eng"}],"publication_status":"published","publication_identifier":{"issn":["1431-0635"]},"intvolume":" 7","month":"01","main_file_link":[{"open_access":"1","url":"https://ems.press/journals/dm/articles/8965058"}],"scopus_import":"1","oa_version":"Published Version","abstract":[{"lang":"eng","text":"Extending work of Bielawski-Dancer 3 and Konno 14, we develop a theory of toric hyperkähler varieties, which involves toric geometry, matroid theory and convex polyhedra. The framework is a detailed study of semi-projective toric varieties, meaning GIT quotients of affine spaces by torus actions, and specifically, of Lawrence toric varieties, meaning GIT quotients of even-dimensional affine spaces by symplectic torus actions. A toric hyperkähler variety is a complete intersection in a Lawrence toric variety. Both varieties are non-compact, and they share the same cohomology ring, namely, the Stanley-Reisner ring of a matroid modulo a linear system of parameters. Familiar applications of toric geometry to combinatorics, including the Hard Lefschetz Theorem and the volume polynomials of Khovanskii-Pukhlikov 11, are extended to the hyperkähler setting. When the matroid is graphic, our construction gives the toric quiver varieties, in the sense of Nakajima 17."}]},{"oa_version":"None","pmid":1,"abstract":[{"lang":"eng","text":"Transcription is a slow and expensive process: in eukaryotes, approximately 20 nucleotides can be transcribed per second at the expense of at least two ATP molecules per nucleotide. Thus, at least for highly expressed genes, transcription of long introns, which are particularly common in mammals, is costly. Using data on the expression of genes that encode proteins in Caenorhabditis elegans and Homo sapiens, we show that introns in highly expressed genes are substantially shorter than those in genes that are expressed at low levels. This difference is greater in humans, such that introns are, on average, 14 times shorter in highly expressed genes than in genes with low expression, whereas in C. Elegans the difference in intron length is only twofold. In contrast, the density of introns in a gene does not strongly depend on the level of gene expression. Thus, natural selection appears to favor short introns in highly expressed genes to minimize the cost of transcription and other molecular processes, such as splicing.\r\n"}],"intvolume":" 31","month":"08","scopus_import":"1","language":[{"iso":"eng"}],"publication_status":"published","volume":31,"issue":"4","_id":"897","status":"public","article_type":"original","type":"journal_article","extern":"1","date_updated":"2023-07-26T09:45:30Z","acknowledgement":"We are grateful to A. Kondrashov, I. Rogozin and A. Feldman for reading the manuscript and P. Bouman, J. Cherry, J. Blumensteil and T. Kim for discussion.","quality_controlled":"1","publisher":"Nature Publishing Group","publication":"Nature Genetics","day":"01","year":"2002","date_created":"2018-12-11T11:49:05Z","date_published":"2002-08-01T00:00:00Z","doi":"10.1038/ng940","page":"415 - 418","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","citation":{"short":"C. Castillo Davis, S. Mekhedov, D. Hartl, E. Koonin, F. Kondrashov, Nature Genetics 31 (2002) 415–418.","ieee":"C. Castillo Davis, S. Mekhedov, D. Hartl, E. Koonin, and F. Kondrashov, “Selection for short introns in highly expressed genes,” Nature Genetics, vol. 31, no. 4. Nature Publishing Group, pp. 415–418, 2002.","apa":"Castillo Davis, C., Mekhedov, S., Hartl, D., Koonin, E., & Kondrashov, F. (2002). Selection for short introns in highly expressed genes. Nature Genetics. Nature Publishing Group. https://doi.org/10.1038/ng940","ama":"Castillo Davis C, Mekhedov S, Hartl D, Koonin E, Kondrashov F. Selection for short introns in highly expressed genes. Nature Genetics. 2002;31(4):415-418. doi:10.1038/ng940","mla":"Castillo Davis, Cristian, et al. “Selection for Short Introns in Highly Expressed Genes.” Nature Genetics, vol. 31, no. 4, Nature Publishing Group, 2002, pp. 415–18, doi:10.1038/ng940.","ista":"Castillo Davis C, Mekhedov S, Hartl D, Koonin E, Kondrashov F. 2002. Selection for short introns in highly expressed genes. Nature Genetics. 31(4), 415–418.","chicago":"Castillo Davis, Cristian, Sergei Mekhedov, Daniel Hartl, Eugene Koonin, and Fyodor Kondrashov. “Selection for Short Introns in Highly Expressed Genes.” Nature Genetics. Nature Publishing Group, 2002. https://doi.org/10.1038/ng940."},"title":"Selection for short introns in highly expressed genes","article_processing_charge":"No","external_id":{"pmid":["12134150"]},"publist_id":"6751","author":[{"full_name":"Castillo Davis, Cristian","last_name":"Castillo Davis","first_name":"Cristian"},{"full_name":"Mekhedov, Sergei","last_name":"Mekhedov","first_name":"Sergei"},{"full_name":"Hartl, Daniel","last_name":"Hartl","first_name":"Daniel"},{"first_name":"Eugene","last_name":"Koonin","full_name":"Koonin, Eugene"},{"id":"44FDEF62-F248-11E8-B48F-1D18A9856A87","first_name":"Fyodor","full_name":"Kondrashov, Fyodor","orcid":"0000-0001-8243-4694","last_name":"Kondrashov"}]},{"scopus_import":"1","main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC65685/","open_access":"1"}],"month":"01","intvolume":" 3","abstract":[{"text":"BACKGROUND: Gene duplications have a major role in the evolution of new biological functions. Theoretical studies often assume that a duplication per se is selectively neutral and that, following a duplication, one of the gene copies is freed from purifying (stabilizing) selection, which creates the potential for evolution of a new function. RESULTS: In search of systematic evidence of accelerated evolution after duplication, we used data from 26 bacterial, six archaeal, and seven eukaryotic genomes to compare the mode and strength of selection acting on recently duplicated genes (paralogs) and on similarly diverged, unduplicated orthologous genes in different species. We find that the ratio of nonsynonymous to synonymous substitutions (Kn/Ks) in most paralogous pairs is <<1 and that paralogs typically evolve at similar rates, without significant asymmetry, indicating that both paralogs produced by a duplication are subject to purifying selection. This selection is, however, substantially weaker than the purifying selection affecting unduplicated orthologs that have diverged to the same extent as the analyzed paralogs. Most of the recently duplicated genes appear to be involved in various forms of environmental response; in particular, many of them encode membrane and secreted proteins. CONCLUSIONS: The results of this analysis indicate that recently duplicated paralogs evolve faster than orthologs with the same level of divergence and similar functions, but apparently do not experience a phase of neutral evolution. We hypothesize that gene duplications that persist in an evolving lineage are beneficial from the time of their origin, due primarily to a protein dosage effect in response to variable environmental conditions; duplications are likely to give rise to new functions at a later phase of their evolution once a higher level of divergence is reached.","lang":"eng"}],"oa_version":"Published Version","pmid":1,"volume":3,"issue":"2","publication_identifier":{"issn":["1465-6906"]},"publication_status":"published","language":[{"iso":"eng"}],"article_type":"original","type":"journal_article","status":"public","_id":"871","date_updated":"2023-07-26T11:48:27Z","extern":"1","publisher":"BioMed Central","quality_controlled":"1","oa":1,"acknowledgement":"We are grateful to A.S. Kondrashov for numerous helpful suggestions, to I. King Jordan, M.A. Roytberg, J.L. Spouge and D.A. Kondrashov for useful discussions and to A.S. Kondrashov, I. King Jordan and D.J. Lipman for critical reading of the manuscript.","doi":"10.1186/gb-2002-3-2-research0008","date_published":"2002-01-01T00:00:00Z","date_created":"2018-12-11T11:48:57Z","year":"2002","day":"01","publication":"Genome Biology","publist_id":"6781","author":[{"orcid":"0000-0001-8243-4694","full_name":"Kondrashov, Fyodor","last_name":"Kondrashov","first_name":"Fyodor","id":"44FDEF62-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Rogozin","full_name":"Rogozin, Igor","first_name":"Igor"},{"last_name":"Wolf","full_name":"Wolf, Yuri","first_name":"Yuri"},{"last_name":"Koonin","full_name":"Koonin, Eugene","first_name":"Eugene"}],"article_processing_charge":"No","external_id":{"pmid":["11864370"]},"title":"Selection in the evolution of gene duplications ","citation":{"ama":"Kondrashov F, Rogozin I, Wolf Y, Koonin E. Selection in the evolution of gene duplications . Genome Biology. 2002;3(2). doi:10.1186/gb-2002-3-2-research0008","apa":"Kondrashov, F., Rogozin, I., Wolf, Y., & Koonin, E. (2002). Selection in the evolution of gene duplications . Genome Biology. BioMed Central. https://doi.org/10.1186/gb-2002-3-2-research0008","ieee":"F. Kondrashov, I. Rogozin, Y. Wolf, and E. Koonin, “Selection in the evolution of gene duplications ,” Genome Biology, vol. 3, no. 2. BioMed Central, 2002.","short":"F. Kondrashov, I. Rogozin, Y. Wolf, E. Koonin, Genome Biology 3 (2002).","mla":"Kondrashov, Fyodor, et al. “Selection in the Evolution of Gene Duplications .” Genome Biology, vol. 3, no. 2, BioMed Central, 2002, doi:10.1186/gb-2002-3-2-research0008.","ista":"Kondrashov F, Rogozin I, Wolf Y, Koonin E. 2002. Selection in the evolution of gene duplications . Genome Biology. 3(2).","chicago":"Kondrashov, Fyodor, Igor Rogozin, Yuri Wolf, and Eugene Koonin. “Selection in the Evolution of Gene Duplications .” Genome Biology. BioMed Central, 2002. https://doi.org/10.1186/gb-2002-3-2-research0008."},"user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17"},{"author":[{"full_name":"Perelygin, Andrey","last_name":"Perelygin","first_name":"Andrey"},{"id":"44FDEF62-F248-11E8-B48F-1D18A9856A87","first_name":"Fyodor","last_name":"Kondrashov","orcid":"0000-0001-8243-4694","full_name":"Kondrashov, Fyodor"},{"last_name":"Rogozin","full_name":"Rogozin, Igor","first_name":"Igor"},{"first_name":"Margo","last_name":"Brinton","full_name":"Brinton, Margo"}],"publist_id":"6787","external_id":{"pmid":["12107596"]},"article_processing_charge":"No","title":"Evolution of the mouse polyubiquitin C gene","citation":{"chicago":"Perelygin, Andrey, Fyodor Kondrashov, Igor Rogozin, and Margo Brinton. “Evolution of the Mouse Polyubiquitin C Gene.” Journal of Molecular Evolution. Springer, 2002. https://doi.org/10.1007/s00239-002-2318-0.","ista":"Perelygin A, Kondrashov F, Rogozin I, Brinton M. 2002. Evolution of the mouse polyubiquitin C gene. Journal of Molecular Evolution. 55(2), 202–210.","mla":"Perelygin, Andrey, et al. “Evolution of the Mouse Polyubiquitin C Gene.” Journal of Molecular Evolution, vol. 55, no. 2, Springer, 2002, pp. 202–10, doi:10.1007/s00239-002-2318-0.","apa":"Perelygin, A., Kondrashov, F., Rogozin, I., & Brinton, M. (2002). Evolution of the mouse polyubiquitin C gene. Journal of Molecular Evolution. Springer. https://doi.org/10.1007/s00239-002-2318-0","ama":"Perelygin A, Kondrashov F, Rogozin I, Brinton M. Evolution of the mouse polyubiquitin C gene. Journal of Molecular Evolution. 2002;55(2):202-210. doi:10.1007/s00239-002-2318-0","ieee":"A. Perelygin, F. Kondrashov, I. Rogozin, and M. Brinton, “Evolution of the mouse polyubiquitin C gene,” Journal of Molecular Evolution, vol. 55, no. 2. Springer, pp. 202–210, 2002.","short":"A. Perelygin, F. Kondrashov, I. Rogozin, M. Brinton, Journal of Molecular Evolution 55 (2002) 202–210."},"user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","publisher":"Springer","quality_controlled":"1","acknowledgement":"We are thankful to J.A. Southerland and P.L. Jiang for technical assistance in DNA sequencing, as well as to Y.I. Pavlov for helpful discussions. This work was supported by public Health Service Research Grant AI45135 from the Institute of Allergy and Infectious Diseases, National Institutes of Health.","page":"202 - 210","doi":"10.1007/s00239-002-2318-0","date_published":"2002-01-01T00:00:00Z","date_created":"2018-12-11T11:48:53Z","year":"2002","day":"01","publication":"Journal of Molecular Evolution","type":"journal_article","article_type":"original","status":"public","_id":"859","date_updated":"2023-07-26T12:01:34Z","extern":"1","scopus_import":"1","month":"01","intvolume":" 55","abstract":[{"text":"The polymeric ubiquitin (poly-u) genes are composed of tandem 228-bp repeats with no spacer sequences between individual monomer units. Ubiquitin is one of the most conserved proteins known to date, and the individual units within a number of poly-u genes are significantly more similar to each other than would be expected if each unit evolved independently. It has been proposed that the rather striking similarity among poly-u monomers in some lineages is caused by a series of homogenization events. Here we report the sequences of the polyubiquitin-C (Ubc) genes in two mouse strains. Analysis of these sequences, as well as those of the previously reported Chinese hamster and rat poly-u genes, supports the assertion that the homogenization of the ubiquitin-C gene in rodents is due to unequal crossing-over events. The sequence divergence of noncoding DNA was used to estimate the frequency of unequal crossing-over events (6.3 x 10-5 events per generation) in the Ubc gene, as well as to provide evidence of apparent selection in the poly-u gene.","lang":"eng"}],"oa_version":"None","pmid":1,"volume":55,"issue":"2","publication_identifier":{"issn":["0022-2844"]},"publication_status":"published","language":[{"iso":"eng"}]},{"title":"Equal Sums of Two kth Powers","article_processing_charge":"No","publist_id":"7708","author":[{"full_name":"Browning, Timothy D","orcid":"0000-0002-8314-0177","last_name":"Browning","id":"35827D50-F248-11E8-B48F-1D18A9856A87","first_name":"Timothy D"}],"user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","citation":{"short":"T.D. Browning, Journal of Number Theory 96 (2002) 293–318.","ieee":"T. D. Browning, “Equal Sums of Two kth Powers,” Journal of Number Theory, vol. 96, no. 2. Academic Press, pp. 293–318, 2002.","apa":"Browning, T. D. (2002). Equal Sums of Two kth Powers. Journal of Number Theory. Academic Press. https://doi.org/10.1006/jnth.2002.2800","ama":"Browning TD. Equal Sums of Two kth Powers. Journal of Number Theory. 2002;96(2):293-318. doi:10.1006/jnth.2002.2800","mla":"Browning, Timothy D. “Equal Sums of Two Kth Powers.” Journal of Number Theory, vol. 96, no. 2, Academic Press, 2002, pp. 293–318, doi:10.1006/jnth.2002.2800.","ista":"Browning TD. 2002. Equal Sums of Two kth Powers. Journal of Number Theory. 96(2), 293–318.","chicago":"Browning, Timothy D. “Equal Sums of Two Kth Powers.” Journal of Number Theory. Academic Press, 2002. https://doi.org/10.1006/jnth.2002.2800."},"date_created":"2018-12-11T11:45:11Z","doi":"10.1006/jnth.2002.2800","date_published":"2002-10-02T00:00:00Z","page":"293 - 318","publication":"Journal of Number Theory","day":"02","year":"2002","publisher":"Academic Press","quality_controlled":"1","extern":"1","date_updated":"2023-07-26T12:15:14Z","status":"public","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"type":"journal_article","article_type":"original","_id":"204","volume":96,"issue":"2","language":[{"iso":"eng"}],"publication_status":"published","publication_identifier":{"issn":["0022-314X"]},"intvolume":" 96","month":"10","scopus_import":"1","oa_version":"Published Version","abstract":[{"text":"Let k⩾5 be an integer, and let x⩾1 be an arbitrary real number. We derive a bound[Formula presented] for the number of positive integers less than or equal to x which can be represented as a sum of two non-negative coprime kth powers, in essentially more than one way.","lang":"eng"}]},{"user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","citation":{"ista":"Fürst J, Ritter M, Rudzki J, Danzl JG, Gschwentner M, Scandella E, Jakab M, König M, Oehl B, Lang F, Deetjen P, Paulmichl M. 2002. ICln Ion channel splice variants in Caenorhabditis elegans. Journal of Biological Chemistry. 277(6), 4435–4445.","chicago":"Fürst, Johannes, Markus Ritter, Jakob Rudzki, Johann G Danzl, Martin Gschwentner, Elke Scandella, Martin Jakab, et al. “ICln Ion Channel Splice Variants in Caenorhabditis Elegans.” Journal of Biological Chemistry. Elsevier, 2002. https://doi.org/10.1074/jbc.m107372200.","ieee":"J. Fürst et al., “ICln Ion channel splice variants in Caenorhabditis elegans,” Journal of Biological Chemistry, vol. 277, no. 6. Elsevier, pp. 4435–4445, 2002.","short":"J. Fürst, M. Ritter, J. Rudzki, J.G. Danzl, M. Gschwentner, E. Scandella, M. Jakab, M. König, B. Oehl, F. Lang, P. Deetjen, M. Paulmichl, Journal of Biological Chemistry 277 (2002) 4435–4445.","ama":"Fürst J, Ritter M, Rudzki J, et al. ICln Ion channel splice variants in Caenorhabditis elegans. Journal of Biological Chemistry. 2002;277(6):4435-4445. doi:10.1074/jbc.m107372200","apa":"Fürst, J., Ritter, M., Rudzki, J., Danzl, J. G., Gschwentner, M., Scandella, E., … Paulmichl, M. (2002). ICln Ion channel splice variants in Caenorhabditis elegans. Journal of Biological Chemistry. Elsevier. https://doi.org/10.1074/jbc.m107372200","mla":"Fürst, Johannes, et al. “ICln Ion Channel Splice Variants in Caenorhabditis Elegans.” Journal of Biological Chemistry, vol. 277, no. 6, Elsevier, 2002, pp. 4435–45, doi:10.1074/jbc.m107372200."},"title":"ICln Ion channel splice variants in Caenorhabditis elegans","author":[{"full_name":"Fürst, Johannes","last_name":"Fürst","first_name":"Johannes"},{"first_name":"Markus","full_name":"Ritter, Markus","last_name":"Ritter"},{"full_name":"Rudzki, Jakob","last_name":"Rudzki","first_name":"Jakob"},{"first_name":"Johann G","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","last_name":"Danzl","orcid":"0000-0001-8559-3973","full_name":"Danzl, Johann G"},{"first_name":"Martin","last_name":"Gschwentner","full_name":"Gschwentner, Martin"},{"first_name":"Elke","full_name":"Scandella, Elke","last_name":"Scandella"},{"first_name":"Martin","full_name":"Jakab, Martin","last_name":"Jakab"},{"last_name":"König","full_name":"König, Matthias","first_name":"Matthias"},{"first_name":"Bernhard","last_name":"Oehl","full_name":"Oehl, Bernhard"},{"first_name":"Florian","last_name":"Lang","full_name":"Lang, Florian"},{"last_name":"Deetjen","full_name":"Deetjen, Peter","first_name":"Peter"},{"full_name":"Paulmichl, Markus","last_name":"Paulmichl","first_name":"Markus"}],"article_processing_charge":"No","external_id":{"pmid":["11706026"]},"acknowledgement":"We are grateful to D. E. Clapham, E. Wöll, G. Meyer, and G. Botta for helpful discussion and/or reading of the manuscript. We also thank T. Stiernagle for providing the N2 strain of C. elegans and A. Wimmer and M. Frick for technical assistance","publisher":"Elsevier","quality_controlled":"1","oa":1,"day":"08","publication":"Journal of Biological Chemistry","has_accepted_license":"1","year":"2002","date_published":"2002-02-08T00:00:00Z","doi":"10.1074/jbc.m107372200","date_created":"2023-08-01T12:37:50Z","page":"4435-4445","_id":"13438","status":"public","keyword":["Cell Biology","Molecular Biology","Biochemistry"],"article_type":"original","type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"extern":"1","ddc":["570"],"date_updated":"2023-08-01T12:55:54Z","file_date_updated":"2023-08-01T12:44:09Z","pmid":1,"oa_version":"Published Version","abstract":[{"lang":"eng","text":"ICln is an ion channel identified by expression cloning using a cDNA library from Madin-Darby canine kidney cells. In all organisms tested so far, only one transcript for the ICln protein could be identified. Here we show that two splice variants of the ICln ion channel can be found in Caenorhabditis elegans. Moreover, we show that these two splice variants of the ICln channel protein, which we termed IClnN1 and IClnN2, can be functionally reconstituted and tested in an artificial lipid bilayer. In these experiments, the IClnN1-induced currents showed no voltage-dependent inactivation, whereas the IClnN2-induced currents fully inactivated at positive potentials. The molecular entity responsible for the voltage-dependent inactivation of IClnN2 is a cluster of positively charged amino acids encoded by exon 2a, which is absent in IClnN1. Our experiments suggest a mechanism of channel inactivation that is similar to the “ball and chain” model proposed for the Shaker potassium channel,i.e. a cluster of positively charged amino acids hinders ion permeation through the channel by a molecular and voltage-dependent interaction at the inner vestibulum of the pore. This hypothesis is supported by the finding that synthetic peptides with the same amino acid sequence as the positive cluster can transform the IClnN1-induced current to the current observed after reconstitution of IClnN2. Furthermore, we show that the nematode ICln gene is embedded in an operon harboring two additional genes, which we termed Nx and Ny. Co-reconstitution of Nx and IClnN2 and functional analysis of the related currents revealed a functional interaction between the two proteins, as evidenced by the fact that the IClnN2-induced current in the presence of Nx was no longer voltage-sensitive. The experiments described indicate that the genome organization in nematodes allows an effective approach for the identification of functional partner proteins of ion channels."}],"month":"02","intvolume":" 277","scopus_import":"1","file":[{"file_id":"13439","checksum":"13abe20f78eb37ab62beb006f62c69b7","success":1,"access_level":"open_access","relation":"main_file","content_type":"application/pdf","date_created":"2023-08-01T12:44:09Z","file_name":"2002_JBC_Fuerst.pdf","creator":"alisjak","date_updated":"2023-08-01T12:44:09Z","file_size":798920}],"language":[{"iso":"eng"}],"publication_identifier":{"issn":["0021-9258"]},"publication_status":"published","volume":277,"issue":"6"}]