[{"month":"12","publication_identifier":{"issn":["0304-3940"]},"main_file_link":[{"url":"https://www.sciencedirect.com/science/article/pii/0304394095122079?via%3Dihub"}],"external_id":{"pmid":["8787837"]},"quality_controlled":"1","doi":"10.1016/0304-3940(95)12207-9","language":[{"iso":"eng"}],"publist_id":"4337","extern":"1","acknowledgement":"We are grateful to Mr. Akira Uesugi for photographic help.","year":"1995","pmid":1,"publication_status":"published","publisher":"Elsevier","author":[{"full_name":"Ohishi, Hitoshi","last_name":"Ohishi","first_name":"Hitoshi"},{"first_name":"Sakashi","last_name":"Nomura","full_name":"Nomura, Sakashi"},{"full_name":"Ding, Yu","first_name":"Yu","last_name":"Ding"},{"first_name":"Ryuichi","last_name":"Shigemoto","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8761-9444","full_name":"Shigemoto, Ryuichi"},{"full_name":"Wada, Eiki","last_name":"Wada","first_name":"Eiki"},{"first_name":"Ayae","last_name":"Kinoshita","full_name":"Kinoshita, Ayae"},{"full_name":"Li, Jin","last_name":"Li","first_name":"Jin"},{"full_name":"Neki, Akio","last_name":"Neki","first_name":"Akio"},{"first_name":"Shigetada","last_name":"Nakanishi","full_name":"Nakanishi, Shigetada"},{"full_name":"Mizuno, Noboru","last_name":"Mizuno","first_name":"Noboru"}],"date_created":"2018-12-11T11:58:24Z","date_updated":"2022-06-28T12:44:57Z","volume":202,"day":"01","article_processing_charge":"No","publication":"Neuroscience Letters","citation":{"ieee":"H. Ohishi et al., “Presynaptic localization of a metabotropic glutamate receptor, mGluR7, in the primary afferent neurons: An immunohistochemical study in the rat,” Neuroscience Letters, vol. 202, no. 1–2. Elsevier, pp. 85–88, 1995.","apa":"Ohishi, H., Nomura, S., Ding, Y., Shigemoto, R., Wada, E., Kinoshita, A., … Mizuno, N. (1995). Presynaptic localization of a metabotropic glutamate receptor, mGluR7, in the primary afferent neurons: An immunohistochemical study in the rat. Neuroscience Letters. Elsevier. https://doi.org/10.1016/0304-3940(95)12207-9","ista":"Ohishi H, Nomura S, Ding Y, Shigemoto R, Wada E, Kinoshita A, Li J, Neki A, Nakanishi S, Mizuno N. 1995. Presynaptic localization of a metabotropic glutamate receptor, mGluR7, in the primary afferent neurons: An immunohistochemical study in the rat. Neuroscience Letters. 202(1–2), 85–88.","ama":"Ohishi H, Nomura S, Ding Y, et al. Presynaptic localization of a metabotropic glutamate receptor, mGluR7, in the primary afferent neurons: An immunohistochemical study in the rat. Neuroscience Letters. 1995;202(1-2):85-88. doi:10.1016/0304-3940(95)12207-9","chicago":"Ohishi, Hitoshi, Sakashi Nomura, Yu Ding, Ryuichi Shigemoto, Eiki Wada, Ayae Kinoshita, Jin Li, Akio Neki, Shigetada Nakanishi, and Noboru Mizuno. “Presynaptic Localization of a Metabotropic Glutamate Receptor, MGluR7, in the Primary Afferent Neurons: An Immunohistochemical Study in the Rat.” Neuroscience Letters. Elsevier, 1995. https://doi.org/10.1016/0304-3940(95)12207-9.","short":"H. Ohishi, S. Nomura, Y. Ding, R. Shigemoto, E. Wada, A. Kinoshita, J. Li, A. Neki, S. Nakanishi, N. Mizuno, Neuroscience Letters 202 (1995) 85–88.","mla":"Ohishi, Hitoshi, et al. “Presynaptic Localization of a Metabotropic Glutamate Receptor, MGluR7, in the Primary Afferent Neurons: An Immunohistochemical Study in the Rat.” Neuroscience Letters, vol. 202, no. 1–2, Elsevier, 1995, pp. 85–88, doi:10.1016/0304-3940(95)12207-9."},"article_type":"original","page":"85 - 88","date_published":"1995-12-01T00:00:00Z","type":"journal_article","abstract":[{"text":"An antibody which recognizes specifically a metabotropic glutamate receptor, mGluR7, was produced by using a trpE fusion protein containing a C-terminal sequence of rat mGluR7. Neuropil in laminae I and II of the dorsal horn of the rat, as well as many neuronal cell bodies in the dorsal root ganglion, showed mGluR7-like immunoreactivity; the immunoreactivity in neuropil was seen in axon terminals, which were filled with round synaptic vesicles and constituted axodendritic and axosomatic asymmetric synapses. The mGluR7-like immunoreactivity in laminae I and II in the dorsal horn was reduced after dorsal rhizotomy. The results indicate that some axon terminals of the primary afferent fibers to laminae I and II of the dorsal horn are provided with mGluR7.","lang":"eng"}],"issue":"1-2","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","_id":"2561","status":"public","title":"Presynaptic localization of a metabotropic glutamate receptor, mGluR7, in the primary afferent neurons: An immunohistochemical study in the rat","intvolume":" 202","oa_version":"None"},{"language":[{"iso":"eng"}],"doi":"10.1016/0304-3940(95)11991-5","quality_controlled":"1","external_id":{"pmid":["8592640"]},"main_file_link":[{"url":"https://www.sciencedirect.com/science/article/pii/0304394095119915?via%3Dihub"}],"month":"09","publication_identifier":{"issn":["0304-3940"]},"date_created":"2018-12-11T11:58:23Z","date_updated":"2022-06-28T12:54:14Z","volume":198,"author":[{"full_name":"Lü, Yan","last_name":"Lü","first_name":"Yan"},{"full_name":"Jin, Shan","last_name":"Jin","first_name":"Shan"},{"first_name":"Tian","last_name":"Xu","full_name":"Xu, Tian"},{"first_name":"Bing","last_name":"Qin","full_name":"Qin, Bing"},{"full_name":"Li, Ji","last_name":"Li","first_name":"Ji"},{"full_name":"Ding, Yu","first_name":"Yu","last_name":"Ding"},{"orcid":"0000-0001-8761-9444","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","last_name":"Shigemoto","first_name":"Ryuichi","full_name":"Shigemoto, Ryuichi"},{"last_name":"Mizuno","first_name":"Noboru","full_name":"Mizuno, Noboru"}],"publication_status":"published","publisher":"Elsevier","year":"1995","pmid":1,"extern":"1","publist_id":"4338","date_published":"1995-09-29T00:00:00Z","article_type":"original","page":"139 - 142","publication":"Neuroscience Letters","citation":{"ista":"Lü Y, Jin S, Xu T, Qin B, Li J, Ding Y, Shigemoto R, Mizuno N. 1995. Expression of c-fos protein in substance P receptor-like immunoreactive neurons in response to noxious stimuli on the urinary bladder: an observation in the lumbosacral cord segments of the rat. Neuroscience Letters. 198(2), 139–142.","apa":"Lü, Y., Jin, S., Xu, T., Qin, B., Li, J., Ding, Y., … Mizuno, N. (1995). Expression of c-fos protein in substance P receptor-like immunoreactive neurons in response to noxious stimuli on the urinary bladder: an observation in the lumbosacral cord segments of the rat. Neuroscience Letters. Elsevier. https://doi.org/10.1016/0304-3940(95)11991-5","ieee":"Y. Lü et al., “Expression of c-fos protein in substance P receptor-like immunoreactive neurons in response to noxious stimuli on the urinary bladder: an observation in the lumbosacral cord segments of the rat,” Neuroscience Letters, vol. 198, no. 2. Elsevier, pp. 139–142, 1995.","ama":"Lü Y, Jin S, Xu T, et al. Expression of c-fos protein in substance P receptor-like immunoreactive neurons in response to noxious stimuli on the urinary bladder: an observation in the lumbosacral cord segments of the rat. Neuroscience Letters. 1995;198(2):139-142. doi:10.1016/0304-3940(95)11991-5","chicago":"Lü, Yan, Shan Jin, Tian Xu, Bing Qin, Ji Li, Yu Ding, Ryuichi Shigemoto, and Noboru Mizuno. “Expression of C-Fos Protein in Substance P Receptor-like Immunoreactive Neurons in Response to Noxious Stimuli on the Urinary Bladder: An Observation in the Lumbosacral Cord Segments of the Rat.” Neuroscience Letters. Elsevier, 1995. https://doi.org/10.1016/0304-3940(95)11991-5.","mla":"Lü, Yan, et al. “Expression of C-Fos Protein in Substance P Receptor-like Immunoreactive Neurons in Response to Noxious Stimuli on the Urinary Bladder: An Observation in the Lumbosacral Cord Segments of the Rat.” Neuroscience Letters, vol. 198, no. 2, Elsevier, 1995, pp. 139–42, doi:10.1016/0304-3940(95)11991-5.","short":"Y. Lü, S. Jin, T. Xu, B. Qin, J. Li, Y. Ding, R. Shigemoto, N. Mizuno, Neuroscience Letters 198 (1995) 139–142."},"day":"29","article_processing_charge":"No","oa_version":"None","status":"public","title":"Expression of c-fos protein in substance P receptor-like immunoreactive neurons in response to noxious stimuli on the urinary bladder: an observation in the lumbosacral cord segments of the rat","intvolume":" 198","_id":"2560","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","abstract":[{"text":"Chemical irritation of the urinary bladder with formalin in the rat induced c-fos protein-like immunoreactivity in more than 80% of substance P receptor-like immunoreactive (SPR-LI) neurons of the dorsal commissural nucleus, sacral parasympathetic nucleus and lamina I in the 6th lumbar and 1st sacral cord segments. These neurons with SPR-LI may receive noxious information from the urinary bladder through the primary afferent fibers with substance P.","lang":"eng"}],"issue":"2","type":"journal_article"},{"publication_identifier":{"issn":["0006-8993"]},"month":"03","language":[{"iso":"eng"}],"doi":"10.1016/0006-8993(95)00022-I","quality_controlled":"1","external_id":{"pmid":["7796113"]},"main_file_link":[{"url":"sciencedirect.com/science/article/pii/000689939500022I?via%3Dihub"}],"extern":"1","publist_id":"4341","volume":674,"date_updated":"2022-06-29T08:32:01Z","date_created":"2018-12-11T11:58:22Z","author":[{"full_name":"Ding, Yu","first_name":"Yu","last_name":"Ding"},{"full_name":"Takada, Masahiko","last_name":"Takada","first_name":"Masahiko"},{"first_name":"Ryuichi","last_name":"Shigemoto","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8761-9444","full_name":"Shigemoto, Ryuichi"},{"full_name":"Mizuno, Noboru","last_name":"Mizuno","first_name":"Noboru"}],"publisher":"Elsevier","publication_status":"published","pmid":1,"year":"1995","acknowledgement":"The authors are grateful for photographic help of Mr. Akira Uesugi. This work was supported in part by Grants-in-Aid for Special Research on Priority Areas 05267104, Scientific Research (B) 05454658, and Scientific Research (C) 06680735 from the Ministry of Education, Science and Culture of Japan.","article_processing_charge":"No","day":"20","scopus_import":"1","date_published":"1995-03-20T00:00:00Z","page":"336 - 340","article_type":"original","citation":{"ista":"Ding Y, Takada M, Shigemoto R, Mizuno N. 1995. Spinoparabrachial tract neurons showing substance P receptor-like immunoreactivity in the lumbar spinal cord of the rat. Brain Research. 674(2), 336–340.","ieee":"Y. Ding, M. Takada, R. Shigemoto, and N. Mizuno, “Spinoparabrachial tract neurons showing substance P receptor-like immunoreactivity in the lumbar spinal cord of the rat,” Brain Research, vol. 674, no. 2. Elsevier, pp. 336–340, 1995.","apa":"Ding, Y., Takada, M., Shigemoto, R., & Mizuno, N. (1995). Spinoparabrachial tract neurons showing substance P receptor-like immunoreactivity in the lumbar spinal cord of the rat. Brain Research. Elsevier. https://doi.org/10.1016/0006-8993(95)00022-I","ama":"Ding Y, Takada M, Shigemoto R, Mizuno N. Spinoparabrachial tract neurons showing substance P receptor-like immunoreactivity in the lumbar spinal cord of the rat. Brain Research. 1995;674(2):336-340. doi:10.1016/0006-8993(95)00022-I","chicago":"Ding, Yu, Masahiko Takada, Ryuichi Shigemoto, and Noboru Mizuno. “Spinoparabrachial Tract Neurons Showing Substance P Receptor-like Immunoreactivity in the Lumbar Spinal Cord of the Rat.” Brain Research. Elsevier, 1995. https://doi.org/10.1016/0006-8993(95)00022-I.","mla":"Ding, Yu, et al. “Spinoparabrachial Tract Neurons Showing Substance P Receptor-like Immunoreactivity in the Lumbar Spinal Cord of the Rat.” Brain Research, vol. 674, no. 2, Elsevier, 1995, pp. 336–40, doi:10.1016/0006-8993(95)00022-I.","short":"Y. Ding, M. Takada, R. Shigemoto, N. Mizuno, Brain Research 674 (1995) 336–340."},"publication":"Brain Research","issue":"2","abstract":[{"text":"By using substance P receptor (SPR) immunofluorescence histochemistry combined with fluorescent retrograde labeling, SPR-like immunoreactive (SPR-LI) neurons sending their axons to the lateral parabrachial region were observed in the lumbar spinal cord of the rat. After injection of Fluoro-Gold into the lateral parabrachial region, retrogradely labeled neurons with SPR-LI were seen frequently in lamina I and the lateral spinal nucleus, and occasionally in laminae IV and V, with a predominantly contralateral distribution. Some of these neurons, especially those in lamina I, may convey nociceptive information to the lateral parabrachial region.","lang":"eng"}],"type":"journal_article","oa_version":"None","intvolume":" 674","status":"public","title":"Spinoparabrachial tract neurons showing substance P receptor-like immunoreactivity in the lumbar spinal cord of the rat","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","_id":"2556"},{"publication_identifier":{"issn":["0168-0102"]},"month":"11","doi":"10.1016/0168-0102(95)00961-R","language":[{"iso":"eng"}],"main_file_link":[{"url":"https://www.sciencedirect.com/science/article/pii/016801029500961R?via%3Dihub"}],"external_id":{"pmid":["8602281"]},"quality_controlled":"1","publist_id":"4336","extern":"1","author":[{"full_name":"Ding, Yu","first_name":"Yu","last_name":"Ding"},{"full_name":"Takada, Masahiko","first_name":"Masahiko","last_name":"Takada"},{"full_name":"Shigemoto, Ryuichi","orcid":"0000-0001-8761-9444","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","last_name":"Shigemoto","first_name":"Ryuichi"},{"first_name":"Noboru","last_name":"Mizuno","full_name":"Mizuno, Noboru"}],"volume":23,"date_updated":"2022-06-28T09:50:42Z","date_created":"2018-12-11T11:58:24Z","pmid":1,"year":"1995","acknowledgement":"The authors are grateful for photographic help of Ms. Miao-Li Zhang and Mr. Akira Uesugi. This work was supported in part by Grants-in-Aid for Special Research on Priority Areas 05267104, Scientific Research (B) 05454658, and Scientific Research (C) 06680735 from the Ministry of Education, Science and Culture of Japan. ","publisher":"Elsevier","publication_status":"published","article_processing_charge":"No","day":"01","date_published":"1995-11-01T00:00:00Z","citation":{"ista":"Ding Y, Takada M, Shigemoto R, Mizuno N. 1995. Trigeminoparabrachial projection neurons showing substance P receptor-like immunoreactivity in the rat. Neuroscience Research. 23(4), 415–418.","ieee":"Y. Ding, M. Takada, R. Shigemoto, and N. Mizuno, “Trigeminoparabrachial projection neurons showing substance P receptor-like immunoreactivity in the rat,” Neuroscience Research, vol. 23, no. 4. Elsevier, pp. 415–418, 1995.","apa":"Ding, Y., Takada, M., Shigemoto, R., & Mizuno, N. (1995). Trigeminoparabrachial projection neurons showing substance P receptor-like immunoreactivity in the rat. Neuroscience Research. Elsevier. https://doi.org/10.1016/0168-0102(95)00961-R","ama":"Ding Y, Takada M, Shigemoto R, Mizuno N. Trigeminoparabrachial projection neurons showing substance P receptor-like immunoreactivity in the rat. Neuroscience Research. 1995;23(4):415-418. doi:10.1016/0168-0102(95)00961-R","chicago":"Ding, Yu, Masahiko Takada, Ryuichi Shigemoto, and Noboru Mizuno. “Trigeminoparabrachial Projection Neurons Showing Substance P Receptor-like Immunoreactivity in the Rat.” Neuroscience Research. Elsevier, 1995. https://doi.org/10.1016/0168-0102(95)00961-R.","mla":"Ding, Yu, et al. “Trigeminoparabrachial Projection Neurons Showing Substance P Receptor-like Immunoreactivity in the Rat.” Neuroscience Research, vol. 23, no. 4, Elsevier, 1995, pp. 415–18, doi:10.1016/0168-0102(95)00961-R.","short":"Y. Ding, M. Takada, R. Shigemoto, N. Mizuno, Neuroscience Research 23 (1995) 415–418."},"publication":"Neuroscience Research","page":"415 - 418","article_type":"original","issue":"4","abstract":[{"text":"By means of substance P receptor (SPR) immunofluorescence histochemistry combined with Fluoro-Gold fluorescent retrograde labeling, SPR-like immunoreactive neurons in the caudal subnucleus of the spinal trigeminal nucleus of the rat were observed to send their axons to the nucleus of Kolliker-Fuse and ventrolateral part of the lateral parabrachial nucleus bilaterally with a clear ipsilateral dominance. These neurons were distributed mainly in lamina I, and additionally in lamina III.","lang":"eng"}],"type":"journal_article","oa_version":"None","_id":"2563","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","intvolume":" 23","status":"public","title":"Trigeminoparabrachial projection neurons showing substance P receptor-like immunoreactivity in the rat"},{"page":"127 - 132","quality_controlled":"1","citation":{"short":"L. Erdös, in:, Birkhäuser, 1995, pp. 127–132.","mla":"Erdös, László. Magnetic Lieb-Thirring Inequalities and Stochastic Oscillatory Integrals. Vol. 78, Birkhäuser, 1995, pp. 127–32, doi:10.1007/978-3-0348-9092-2_13.","chicago":"Erdös, László. “Magnetic Lieb-Thirring Inequalities and Stochastic Oscillatory Integrals,” 78:127–32. Birkhäuser, 1995. https://doi.org/10.1007/978-3-0348-9092-2_13.","ama":"Erdös L. Magnetic Lieb-Thirring inequalities and stochastic oscillatory integrals. In: Vol 78. Birkhäuser; 1995:127-132. doi:10.1007/978-3-0348-9092-2_13","apa":"Erdös, L. (1995). Magnetic Lieb-Thirring inequalities and stochastic oscillatory integrals (Vol. 78, pp. 127–132). Presented at the International Conference on Partial Differential Operators and Mathematical Physics, Holzhau, Germany: Birkhäuser. https://doi.org/10.1007/978-3-0348-9092-2_13","ieee":"L. Erdös, “Magnetic Lieb-Thirring inequalities and stochastic oscillatory integrals,” presented at the International Conference on Partial Differential Operators and Mathematical Physics, Holzhau, Germany, 1995, vol. 78, pp. 127–132.","ista":"Erdös L. 1995. Magnetic Lieb-Thirring inequalities and stochastic oscillatory integrals. International Conference on Partial Differential Operators and Mathematical Physics, Operator Theory: Advances and Applications, vol. 78, 127–132."},"main_file_link":[{"url":"https://link.springer.com/chapter/10.1007/978-3-0348-9092-2_13"}],"language":[{"iso":"eng"}],"doi":"10.1007/978-3-0348-9092-2_13","date_published":"1995-01-01T00:00:00Z","conference":{"name":"International Conference on Partial Differential Operators and Mathematical Physics","end_date":"1995-07-09","start_date":"1995-07-03","location":"Holzhau, Germany"},"article_processing_charge":"No","month":"01","day":"01","intvolume":" 78","publisher":"Birkhäuser","title":"Magnetic Lieb-Thirring inequalities and stochastic oscillatory integrals","publication_status":"published","status":"public","_id":"2712","year":"1995","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","acknowledgement":"Work supported by the NSF grant PHY90-19433 A02, by the Alfred Sloan Foundation dissertation Fellowship and by the Erwin Schrödinger Institute for Mathematical Physics in Vienna.","volume":78,"oa_version":"None","date_created":"2018-12-11T11:59:12Z","date_updated":"2022-06-28T09:31:20Z","author":[{"full_name":"Erdös, László","orcid":"0000-0001-5366-9603","id":"4DBD5372-F248-11E8-B48F-1D18A9856A87","last_name":"Erdös","first_name":"László"}],"alternative_title":["Operator Theory: Advances and Applications"],"type":"conference","extern":"1","publist_id":"4184","abstract":[{"text":"We study the generalizations of the well-known Lieb-Thirring inequality for the magnetic Schrödinger operator with a nonconstant magnetic field. We use stochastic methods to prove estimates on the moments of the negative eigenvalues.","lang":"eng"}]},{"date_published":"1995-06-01T00:00:00Z","citation":{"ama":"Erdös L. Magnetic Lieb-Thirring inequalities. Communications in Mathematical Physics. 1995;170(3):629-668. doi:10.1007/BF02099152","ista":"Erdös L. 1995. Magnetic Lieb-Thirring inequalities. Communications in Mathematical Physics. 170(3), 629–668.","ieee":"L. Erdös, “Magnetic Lieb-Thirring inequalities,” Communications in Mathematical Physics, vol. 170, no. 3. Springer, pp. 629–668, 1995.","apa":"Erdös, L. (1995). Magnetic Lieb-Thirring inequalities. Communications in Mathematical Physics. Springer. https://doi.org/10.1007/BF02099152","mla":"Erdös, László. “Magnetic Lieb-Thirring Inequalities.” Communications in Mathematical Physics, vol. 170, no. 3, Springer, 1995, pp. 629–68, doi:10.1007/BF02099152.","short":"L. Erdös, Communications in Mathematical Physics 170 (1995) 629–668.","chicago":"Erdös, László. “Magnetic Lieb-Thirring Inequalities.” Communications in Mathematical Physics. Springer, 1995. https://doi.org/10.1007/BF02099152."},"publication":"Communications in Mathematical Physics","page":"629 - 668","article_type":"original","article_processing_charge":"No","day":"01","oa_version":"None","_id":"2724","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","intvolume":" 170","status":"public","title":"Magnetic Lieb-Thirring inequalities","issue":"3","abstract":[{"text":"We study the generalizations of the well-known Lieb-Thirring inequality for the magnetic Schrödinger operator with nonconstant magnetic field. Our main result is the naturally expected magnetic Lieb-Thirring estimate on the moments of the negative eigenvalues for a certain class of magnetic fields (including even some unbounded ones). We develop a localization technique in path space of the stochastic Feynman-Kac representation of the heat kernel which effectively estimates the oscillatory effect due to the magnetic phase factor.","lang":"eng"}],"type":"journal_article","doi":"10.1007/BF02099152","language":[{"iso":"eng"}],"main_file_link":[{"url":"https://link.springer.com/article/10.1007/BF02099152"}],"quality_controlled":"1","publication_identifier":{"issn":["0010-3616"]},"month":"06","author":[{"full_name":"Erdös, László","id":"4DBD5372-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5366-9603","first_name":"László","last_name":"Erdös"}],"volume":170,"date_created":"2018-12-11T11:59:16Z","date_updated":"2022-06-28T09:19:36Z","year":"1995","publisher":"Springer","publication_status":"published","publist_id":"4168","extern":"1"},{"author":[{"last_name":"Monyer","first_name":"Hannah","full_name":"Monyer, Hannah"},{"full_name":"Jonas, Peter M","first_name":"Peter M","last_name":"Jonas","id":"353C1B58-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5001-4804"}],"oa_version":"None","date_updated":"2022-06-28T09:13:01Z","date_created":"2018-12-11T12:03:25Z","year":"1995","_id":"3454","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","publisher":"Plenum","editor":[{"last_name":"Sakmann","first_name":"Bert","full_name":"Sakmann, Bert"},{"last_name":"Neher","first_name":"Erwin","full_name":"Neher, Erwin"}],"title":"Polymerase chain reaction analysis of ion channel expression in single neurons of brain slices","publication_status":"published","status":"public","publist_id":"2933","abstract":[{"text":"The study of gene expression and regulation in the central nervous system (CNS) is a daunting task because of the diversity of neuronal phenotypes and the complexity of many protein classes. Molecular cloning revealed the presence of a large number of different protein families in the CNS, each comprising several members. Ligand-gated ion channels may serve as an example to illustrate this point (for review, see Unwin, 1993). Heterologous expression combined with electrophysiological analysis suggests that ligand-gated channels are multimeric proteins with functional properties depending on the subunit composition. Very little is known, however, about how the functional properties of the recombinant and native receptors relate to each other. Thus, it is of eminent importance to elucidate the subunit expression profile in different types of neurons in the CNS and to correlate this with the functional properties of the native receptors.","lang":"eng"}],"extern":"1","type":"book_chapter","doi":"10.1007/978-1-4419-1229-9_16","date_published":"1995-01-01T00:00:00Z","language":[{"iso":"eng"}],"citation":{"chicago":"Monyer, Hannah, and Peter M Jonas. “Polymerase Chain Reaction Analysis of Ion Channel Expression in Single Neurons of Brain Slices.” In Single-Channel Recording, edited by Bert Sakmann and Erwin Neher, 357–73. Plenum, 1995. https://doi.org/10.1007/978-1-4419-1229-9_16.","mla":"Monyer, Hannah, and Peter M. Jonas. “Polymerase Chain Reaction Analysis of Ion Channel Expression in Single Neurons of Brain Slices.” Single-Channel Recording, edited by Bert Sakmann and Erwin Neher, Plenum, 1995, pp. 357–73, doi:10.1007/978-1-4419-1229-9_16.","short":"H. Monyer, P.M. Jonas, in:, B. Sakmann, E. Neher (Eds.), Single-Channel Recording, Plenum, 1995, pp. 357–373.","ista":"Monyer H, Jonas PM. 1995.Polymerase chain reaction analysis of ion channel expression in single neurons of brain slices. In: Single-channel recording. , 357–373.","apa":"Monyer, H., & Jonas, P. M. (1995). Polymerase chain reaction analysis of ion channel expression in single neurons of brain slices. In B. Sakmann & E. Neher (Eds.), Single-channel recording (pp. 357–373). Plenum. https://doi.org/10.1007/978-1-4419-1229-9_16","ieee":"H. Monyer and P. M. Jonas, “Polymerase chain reaction analysis of ion channel expression in single neurons of brain slices,” in Single-channel recording, B. Sakmann and E. Neher, Eds. Plenum, 1995, pp. 357–373.","ama":"Monyer H, Jonas PM. Polymerase chain reaction analysis of ion channel expression in single neurons of brain slices. In: Sakmann B, Neher E, eds. Single-Channel Recording. Plenum; 1995:357-373. doi:10.1007/978-1-4419-1229-9_16"},"main_file_link":[{"url":"https://link.springer.com/chapter/10.1007/978-1-4419-1229-9_16"}],"publication":"Single-channel recording","page":"357 - 373","quality_controlled":"1","article_processing_charge":"No","publication_identifier":{"isbn":["978-0-306-44870-6"]},"month":"01","day":"01"},{"author":[{"full_name":"Jonas, Peter M","first_name":"Peter M","last_name":"Jonas","id":"353C1B58-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5001-4804"}],"date_updated":"2022-06-28T08:51:40Z","date_created":"2018-12-11T12:03:25Z","oa_version":"None","_id":"3455","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","year":"1995","status":"public","publication_status":"published","title":"Fast application of agonists to isolated membrane patches","publisher":"Plenum","editor":[{"full_name":"Sakmann, Bert","last_name":"Sakmann","first_name":"Bert"},{"first_name":"Erwin","last_name":"Neher","full_name":"Neher, Erwin"}],"abstract":[{"lang":"eng","text":"At a synapse, the transmitter is stored in synaptic vesicles and is released into the synaptic cleft almost instantaneously upon fusion of these vesicles with the presynaptic membrane. Subsequently, the transmitter diffuses to ligand-gated ion channels in the postsynaptic density, binds to them, and thereby causes channel activation. Unfortunately, we have estimates neither of the exact amount of transmitter in the synaptic vesicle nor of the concentration in the synaptic cleft reaching the postsynaptic receptors, and in some cases even the identity of the transmitter is unknown. These questions may be addressed by modeling of release and diffusion. Such a theoretical approach, however, is based on several assumptions, some of which lack experimental evidence."}],"publist_id":"2932","extern":"1","type":"book_chapter","doi":"10.1007/978-1-4419-1229-9_10","date_published":"1995-01-01T00:00:00Z","language":[{"iso":"eng"}],"publication":"Single-channel recording","main_file_link":[{"url":"https://link.springer.com/chapter/10.1007/978-1-4419-1229-9_10"}],"citation":{"ama":"Jonas PM. Fast application of agonists to isolated membrane patches. In: Sakmann B, Neher E, eds. Single-Channel Recording. Plenum; 1995:231-243. doi:10.1007/978-1-4419-1229-9_10","apa":"Jonas, P. M. (1995). Fast application of agonists to isolated membrane patches. In B. Sakmann & E. Neher (Eds.), Single-channel recording (pp. 231–243). Plenum. https://doi.org/10.1007/978-1-4419-1229-9_10","ieee":"P. M. Jonas, “Fast application of agonists to isolated membrane patches,” in Single-channel recording, B. Sakmann and E. Neher, Eds. Plenum, 1995, pp. 231–243.","ista":"Jonas PM. 1995.Fast application of agonists to isolated membrane patches. In: Single-channel recording. , 231–243.","short":"P.M. Jonas, in:, B. Sakmann, E. Neher (Eds.), Single-Channel Recording, Plenum, 1995, pp. 231–243.","mla":"Jonas, Peter M. “Fast Application of Agonists to Isolated Membrane Patches.” Single-Channel Recording, edited by Bert Sakmann and Erwin Neher, Plenum, 1995, pp. 231–43, doi:10.1007/978-1-4419-1229-9_10.","chicago":"Jonas, Peter M. “Fast Application of Agonists to Isolated Membrane Patches.” In Single-Channel Recording, edited by Bert Sakmann and Erwin Neher, 231–43. Plenum, 1995. https://doi.org/10.1007/978-1-4419-1229-9_10."},"quality_controlled":"1","page":"231 - 243","month":"01","day":"01","article_processing_charge":"No","publication_identifier":{"isbn":["978-0-306-44870-6"]}},{"issue":"5","type":"journal_article","oa_version":"Published Version","intvolume":" 15","title":"Molecular mechanisms controlling calcium entry through AMPA-type glutamate receptor channels","status":"public","_id":"3461","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","article_processing_charge":"No","day":"01","scopus_import":"1","date_published":"1995-11-01T00:00:00Z","page":"987 - 990","article_type":"original","citation":{"apa":"Jonas, P. M., & Burnashev, N. (1995). Molecular mechanisms controlling calcium entry through AMPA-type glutamate receptor channels. Neuron. Elsevier. https://doi.org/10.1016/0896-6273(95)90087-X","ieee":"P. M. Jonas and N. Burnashev, “Molecular mechanisms controlling calcium entry through AMPA-type glutamate receptor channels,” Neuron, vol. 15, no. 5. Elsevier, pp. 987–990, 1995.","ista":"Jonas PM, Burnashev N. 1995. Molecular mechanisms controlling calcium entry through AMPA-type glutamate receptor channels. Neuron. 15(5), 987–990.","ama":"Jonas PM, Burnashev N. Molecular mechanisms controlling calcium entry through AMPA-type glutamate receptor channels. Neuron. 1995;15(5):987-990. doi:10.1016/0896-6273(95)90087-X","chicago":"Jonas, Peter M, and Nail Burnashev. “Molecular Mechanisms Controlling Calcium Entry through AMPA-Type Glutamate Receptor Channels.” Neuron. Elsevier, 1995. https://doi.org/10.1016/0896-6273(95)90087-X.","short":"P.M. Jonas, N. Burnashev, Neuron 15 (1995) 987–990.","mla":"Jonas, Peter M., and Nail Burnashev. “Molecular Mechanisms Controlling Calcium Entry through AMPA-Type Glutamate Receptor Channels.” Neuron, vol. 15, no. 5, Elsevier, 1995, pp. 987–90, doi:10.1016/0896-6273(95)90087-X."},"publication":"Neuron","extern":"1","publist_id":"2926","volume":15,"date_created":"2018-12-11T12:03:27Z","date_updated":"2022-06-28T08:34:36Z","author":[{"full_name":"Jonas, Peter M","orcid":"0000-0001-5001-4804","id":"353C1B58-F248-11E8-B48F-1D18A9856A87","last_name":"Jonas","first_name":"Peter M"},{"full_name":"Burnashev, Nail","last_name":"Burnashev","first_name":"Nail"}],"publisher":"Elsevier","publication_status":"published","pmid":1,"year":"1995","publication_identifier":{"issn":["0896-6273"]},"month":"11","language":[{"iso":"eng"}],"doi":"10.1016/0896-6273(95)90087-X","quality_controlled":"1","main_file_link":[{"url":"https://www.sciencedirect.com/science/article/pii/089662739590087X?via%3Dihub","open_access":"1"}],"oa":1,"external_id":{"pmid":["7576666"]}},{"quality_controlled":"1","external_id":{"pmid":["7536248"]},"main_file_link":[{"open_access":"1","url":"https://physoc.onlinelibrary.wiley.com/doi/abs/10.1113/jphysiol.1995.sp020521"}],"oa":1,"language":[{"iso":"eng"}],"doi":"10.1113/jphysiol.1995.sp020521","publication_identifier":{"issn":["0022-3751"]},"month":"01","publisher":"Wiley-Blackwell","publication_status":"published","pmid":1,"acknowledgement":"We thank M.Hausser, A.Roth, P.Ruppersberg, and G.Stuart for helpful discussions and M.H. and G.S. for critically reading the manuscript. We also thank M.Kaiser for expert technical assistance and F.Helmchen, M.Huke and A.Roth for computer programming. Financial support from the Alexander von Humboldt Foundation and the Deutsche Forschungsgemeinschaft (SFB317) is gratefully acknowledged.","year":"1995","volume":482,"date_updated":"2022-06-28T08:08:40Z","date_created":"2018-12-11T12:03:32Z","author":[{"full_name":"Spruston, Nelson","last_name":"Spruston","first_name":"Nelson"},{"full_name":"Jonas, Peter M","last_name":"Jonas","first_name":"Peter M","orcid":"0000-0001-5001-4804","id":"353C1B58-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Bert","last_name":"Sakmann","full_name":"Sakmann, Bert"}],"extern":"1","publist_id":"2909","page":"325 - 352","article_type":"original","citation":{"apa":"Spruston, N., Jonas, P. M., & Sakmann, B. (1995). Dendritic glutamate receptor channels in rat hippocampal CA3 and CA1 pyramidal neurons. Journal of Physiology. Wiley-Blackwell. https://doi.org/10.1113/jphysiol.1995.sp020521","ieee":"N. Spruston, P. M. Jonas, and B. Sakmann, “Dendritic glutamate receptor channels in rat hippocampal CA3 and CA1 pyramidal neurons,” Journal of Physiology, vol. 482, no. Pt 2. Wiley-Blackwell, pp. 325–352, 1995.","ista":"Spruston N, Jonas PM, Sakmann B. 1995. Dendritic glutamate receptor channels in rat hippocampal CA3 and CA1 pyramidal neurons. Journal of Physiology. 482(Pt 2), 325–352.","ama":"Spruston N, Jonas PM, Sakmann B. Dendritic glutamate receptor channels in rat hippocampal CA3 and CA1 pyramidal neurons. Journal of Physiology. 1995;482(Pt 2):325-352. doi:10.1113/jphysiol.1995.sp020521","chicago":"Spruston, Nelson, Peter M Jonas, and Bert Sakmann. “Dendritic Glutamate Receptor Channels in Rat Hippocampal CA3 and CA1 Pyramidal Neurons.” Journal of Physiology. Wiley-Blackwell, 1995. https://doi.org/10.1113/jphysiol.1995.sp020521.","short":"N. Spruston, P.M. Jonas, B. Sakmann, Journal of Physiology 482 (1995) 325–352.","mla":"Spruston, Nelson, et al. “Dendritic Glutamate Receptor Channels in Rat Hippocampal CA3 and CA1 Pyramidal Neurons.” Journal of Physiology, vol. 482, no. Pt 2, Wiley-Blackwell, 1995, pp. 325–52, doi:10.1113/jphysiol.1995.sp020521."},"publication":"Journal of Physiology","date_published":"1995-01-15T00:00:00Z","article_processing_charge":"No","day":"15","intvolume":" 482","status":"public","title":"Dendritic glutamate receptor channels in rat hippocampal CA3 and CA1 pyramidal neurons","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","_id":"3478","oa_version":"Published Version","type":"journal_article","issue":"Pt 2","abstract":[{"text":"1. Properties of dendritic glutamate receptor (GluR) channels were investigated using fast application of glutamate to outside-out membrane patches isolated from the apical dendrites of CA3 and CA1 pyramidal neurons in rat hippocampal slices. CA3 patches were formed (15-76 μm from the soma) in the region of messy fibre (MF) synapses, and CA1 patches (25-174 μm from the soma) in the region of Schaffer collateral (SC) innervation. 2. Dual-component responses consisting of a rapidly rising and decaying component followed by a second, substantially slower, component were elicited by 1 ms pulses of 1 mM glutamate in the presence of 10 μM glycine and absence of external Mg2+. The fast component was selectively blocked by 2-5 μM 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and the slow component by 30 μM D-2-amino-5-phosphonopentanoic acid (D-AP5), suggesting that the fast and slow components were mediated by the GluR channels of the L-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) and NMDA type, respectively. The peak amplitude ratio of the NMDA to AMPA receptor-mediated components varied between 0.03 and 0.62 in patches from both CA3 and CA1 dendrites. Patches lacking either component were rarely observed. 3. The peak current-voltage (I-V) relationship of the fast component was almost linear, whereas the I-V relationship of the slow component showed a region of negative slope in the presence of 1 mM external Mg2+. The reversal potential for both components was close to 0 mV. 4. Kainate-preferring GluR channels did not contribute appreciably to the response to glutamate. The responses to 100 ms pulses of 1 mM glutamate were mimicked by application of 1 mM AMPA, whereas 1 mM kainate produced much smaller, weakly desensitizing currents. This suggests that the fast component is primarily mediated by the action of glutamate on AMPA-preferring receptors. 5. The mean elementary conductance of AMPA receptor channels was about 10 pS, as estimated by non-stationary fluctuation analysis. The permeability of these channels to Ca2+ was low (~5% of the permeability to Cs+). 6. The elementary conductance of NMDA receptor channels was larger, with a main conductance state of about 45 pS. These channels were 3.6 times more permeable to Ca2+ than to Cs+. 7. AMPA receptor-mediated currents activated rapidly in response to 1 ms pulses of 1 mM glutamate and deactivated with a predominant, fast time constant and a smaller, slower component (τ1≃2 ms, τ2≃8 ms, contributing ~80 and ~20% to the total decay amplitude, respectively). Desensitization of the current during a 100 ms pulse was best fitted by two time constants (τ1≃10 ms, ~60%; τ2≃34 ms, ~40%). 8. NMDA receptor-mediated currents in response to 1 ms pulses of 1 mM glutamate activated and deactivated much more slowly than AMPA receptor-mediated currents. The time course could be described by a single exponential rising phase (τ≃7 ms) followed by a double exponential decay (τ1≃200 ms, ~80%; τ2≃1-3 s, ~20%). 9. Mg2+ blocked the NMDA component in a voltage-dependent manner, with a half-maximal inhibitory concentration (IC50) of 21 μM at -80 mV. At physiological Mg2+ concentrations, block of the NMDA component could be rapidly relieved with voltage jumps from negative to positive potentials. Block of the current upon return to negative potentials occurred almost instantaneously. 10. Zn2+ also selectively-blocked the NMDA receptor-mediated current with an IC50 of 22 μM, but this block differed from that of Mg2+ in that it showed little voltage dependence. Rapid application of Zn2+ together with glutamate produced partial block of the current. More block was observed if Zn2+ and glutamate were co-applied when NMDA receptor channels were already open. 11. The functional properties of dendritic GluRs were similar to those found at the soma. Knowledge of these properties facilitated simulations investigating the contribution of coactivated AMPA and NMDA receptors to synaptic depolarization and Ca2+ entry into dendritic spines. Because of its slow deactivation, the NMDA receptor-mediated current contributes substantially to depolarization and Ca2+ entry and is susceptible to modulation over a period of seconds, either by backpropagating action potentials or by the release of Zn2+ from presynaptic boutons.","lang":"eng"}]}]