---
_id: '197'
abstract:
- lang: eng
text: Modern computer vision systems heavily rely on statistical machine learning
models, which typically require large amounts of labeled data to be learned reliably.
Moreover, very recently computer vision research widely adopted techniques for
representation learning, which further increase the demand for labeled data. However,
for many important practical problems there is relatively small amount of labeled
data available, so it is problematic to leverage full potential of the representation
learning methods. One way to overcome this obstacle is to invest substantial resources
into producing large labelled datasets. Unfortunately, this can be prohibitively
expensive in practice. In this thesis we focus on the alternative way of tackling
the aforementioned issue. We concentrate on methods, which make use of weakly-labeled
or even unlabeled data. Specifically, the first half of the thesis is dedicated
to the semantic image segmentation task. We develop a technique, which achieves
competitive segmentation performance and only requires annotations in a form of
global image-level labels instead of dense segmentation masks. Subsequently, we
present a new methodology, which further improves segmentation performance by
leveraging tiny additional feedback from a human annotator. By using our methods
practitioners can greatly reduce the amount of data annotation effort, which is
required to learn modern image segmentation models. In the second half of the
thesis we focus on methods for learning from unlabeled visual data. We study a
family of autoregressive models for modeling structure of natural images and discuss
potential applications of these models. Moreover, we conduct in-depth study of
one of these applications, where we develop the state-of-the-art model for the
probabilistic image colorization task.
acknowledgement: I also gratefully acknowledge the support of NVIDIA Corporation with
the donation of the GPUs used for this research.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Alexander
full_name: Kolesnikov, Alexander
id: 2D157DB6-F248-11E8-B48F-1D18A9856A87
last_name: Kolesnikov
citation:
ama: Kolesnikov A. Weakly-Supervised Segmentation and Unsupervised Modeling of Natural
Images. 2018. doi:10.15479/AT:ISTA:th_1021
apa: Kolesnikov, A. (2018). Weakly-Supervised Segmentation and Unsupervised Modeling
of Natural Images. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:th_1021
chicago: Kolesnikov, Alexander. “Weakly-Supervised Segmentation and Unsupervised
Modeling of Natural Images.” Institute of Science and Technology Austria, 2018.
https://doi.org/10.15479/AT:ISTA:th_1021.
ieee: A. Kolesnikov, “Weakly-Supervised Segmentation and Unsupervised Modeling of
Natural Images,” Institute of Science and Technology Austria, 2018.
ista: Kolesnikov A. 2018. Weakly-Supervised Segmentation and Unsupervised Modeling
of Natural Images. Institute of Science and Technology Austria.
mla: Kolesnikov, Alexander. Weakly-Supervised Segmentation and Unsupervised Modeling
of Natural Images. Institute of Science and Technology Austria, 2018, doi:10.15479/AT:ISTA:th_1021.
short: A. Kolesnikov, Weakly-Supervised Segmentation and Unsupervised Modeling of
Natural Images, Institute of Science and Technology Austria, 2018.
date_created: 2018-12-11T11:45:09Z
date_published: 2018-05-25T00:00:00Z
date_updated: 2023-09-07T12:51:46Z
day: '25'
ddc:
- '004'
degree_awarded: PhD
department:
- _id: ChLa
doi: 10.15479/AT:ISTA:th_1021
ec_funded: 1
file:
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checksum: bc678e02468d8ebc39dc7267dfb0a1c4
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:14:57Z
date_updated: 2020-07-14T12:45:22Z
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date_updated: 2020-07-14T12:45:22Z
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has_accepted_license: '1'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
page: '113'
project:
- _id: 2532554C-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '308036'
name: Lifelong Learning of Visual Scene Understanding
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '7718'
pubrep_id: '1021'
status: public
supervisor:
- first_name: Christoph
full_name: Lampert, Christoph
id: 40C20FD2-F248-11E8-B48F-1D18A9856A87
last_name: Lampert
orcid: 0000-0001-8622-7887
title: Weakly-Supervised Segmentation and Unsupervised Modeling of Natural Images
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2018'
...
---
_id: '200'
abstract:
- lang: eng
text: This thesis is concerned with the inference of current population structure
based on geo-referenced genetic data. The underlying idea is that population structure
affects its spatial genetic structure. Therefore, genotype information can be
utilized to estimate important demographic parameters such as migration rates.
These indirect estimates of population structure have become very attractive,
as genotype data is now widely available. However, there also has been much concern
about these approaches. Importantly, genetic structure can be influenced by many
complex patterns, which often cannot be disentangled. Moreover, many methods merely
fit heuristic patterns of genetic structure, and do not build upon population
genetics theory. Here, I describe two novel inference methods that address these
shortcomings. In Chapter 2, I introduce an inference scheme based on a new type
of signal, identity by descent (IBD) blocks. Recently, it has become feasible
to detect such long blocks of genome shared between pairs of samples. These blocks
are direct traces of recent coalescence events. As such, they contain ample signal
for inferring recent demography. I examine sharing of IBD blocks in two-dimensional
populations with local migration. Using a diffusion approximation, I derive formulas
for an isolation by distance pattern of long IBD blocks and show that sharing
of long IBD blocks approaches rapid exponential decay for growing sample distance.
I describe an inference scheme based on these results. It can robustly estimate
the dispersal rate and population density, which is demonstrated on simulated
data. I also show an application to estimate mean migration and the rate of recent
population growth within Eastern Europe. Chapter 3 is about a novel method to
estimate barriers to gene flow in a two dimensional population. This inference
scheme utilizes geographically localized allele frequency fluctuations - a classical
isolation by distance signal. The strength of these local fluctuations increases
on average next to a barrier, and there is less correlation across it. I again
use a framework of diffusion of ancestral lineages to model this effect, and provide
an efficient numerical implementation to fit the results to geo-referenced biallelic
SNP data. This inference scheme is able to robustly estimate strong barriers to
gene flow, as tests on simulated data confirm.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Harald
full_name: Ringbauer, Harald
id: 417FCFF4-F248-11E8-B48F-1D18A9856A87
last_name: Ringbauer
orcid: 0000-0002-4884-9682
citation:
ama: Ringbauer H. Inferring recent demography from spatial genetic structure. 2018.
doi:10.15479/AT:ISTA:th_963
apa: Ringbauer, H. (2018). Inferring recent demography from spatial genetic structure.
Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:th_963
chicago: Ringbauer, Harald. “Inferring Recent Demography from Spatial Genetic Structure.”
Institute of Science and Technology Austria, 2018. https://doi.org/10.15479/AT:ISTA:th_963.
ieee: H. Ringbauer, “Inferring recent demography from spatial genetic structure,”
Institute of Science and Technology Austria, 2018.
ista: Ringbauer H. 2018. Inferring recent demography from spatial genetic structure.
Institute of Science and Technology Austria.
mla: Ringbauer, Harald. Inferring Recent Demography from Spatial Genetic Structure.
Institute of Science and Technology Austria, 2018, doi:10.15479/AT:ISTA:th_963.
short: H. Ringbauer, Inferring Recent Demography from Spatial Genetic Structure,
Institute of Science and Technology Austria, 2018.
date_created: 2018-12-11T11:45:10Z
date_published: 2018-02-21T00:00:00Z
date_updated: 2023-09-20T12:00:56Z
day: '21'
ddc:
- '576'
degree_awarded: PhD
department:
- _id: NiBa
doi: 10.15479/AT:ISTA:th_963
file:
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checksum: 8cc534d2b528ae017acf80874cce48c9
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creator: dernst
date_created: 2019-04-05T09:30:12Z
date_updated: 2020-07-14T12:45:23Z
file_id: '6224'
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file_size: 113365
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language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc/4.0/
month: '02'
oa: 1
oa_version: Published Version
page: '146'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '7713'
pubrep_id: '963'
related_material:
record:
- id: '563'
relation: part_of_dissertation
status: public
- id: '1074'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Nicholas H
full_name: Barton, Nicholas H
id: 4880FE40-F248-11E8-B48F-1D18A9856A87
last_name: Barton
orcid: 0000-0002-8548-5240
title: Inferring recent demography from spatial genetic structure
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2018'
...
---
_id: '418'
abstract:
- lang: eng
text: "The aim of this thesis was the development of new strategies for optical
and optogenetic control of proliferative and pro-survival signaling, and characterizing
them from the molecular mechanism up to cellular effects. These new light-based
methods have unique features, such as red light as an activator, or the avoidance
of gene delivery, which enable to overcome current limitations, such as light
delivery to target tissues and feasibility as therapeutic approach. A special
focus was placed on implementing these new light-based approaches in pancreatic
β-cells, as β-cells are the key players in diabetes and especially their loss
in number negatively affects disease progression. Currently no treatment options
are available to compensate the lack of functional β-cells in diabetic patients.\r\nIn
a first approach, red-light-activated growth factor receptors, in particular receptor
tyrosine kinases were engineered and characterized. Receptor activation with light
allows spatio-temporal control compared to ligand-based activation, and especially
red light exhibits deeper tissue penetration than other wavelengths of the visible
spectrum. Red-light-activated receptor tyrosine kinases robustly activated major
growth factor related signaling pathways with a high temporal resolution. Moreover,
the remote activation of the proliferative MAPK/Erk pathway by red-light-activated
receptor tyrosine kinases in a pancreatic β-cell line was also achieved, through
one centimeter thick mouse tissue. Although red-light-activated receptor tyrosine
kinases are particularly attractive for applications in animal models due to the
deep tissue penetration of red light, a drawback, especially with regard to translation
into humans, is the requirement of gene therapy.\r\nIn a second approach an endogenous
light-sensitive mechanism was identified and its potential to promote proliferative
and pro-survival signals was explored, towards light-based tissue regeneration
without the need for gene transfer. Blue-green light illumination was found to
be sufficient for the activation of proliferation and survival promoting signaling
pathways in primary pancreatic murine and human islets. Blue-green light also
led to an increase in proliferation of primary islet cells, an effect which was
shown to be mostly β-cell specific in human islets. Moreover, it was demonstrated
that this approach of pancreatic β-cell expansion did not have any negative effect
on the β-cell function, in particular on their insulin secretion capacity. In
contrast, a trend for enhanced insulin secretion under high glucose conditions
after illumination was detected. In order to unravel the detailed characteristics
of this endogenous light-sensitive mechanism, the precise light requirements were
determined. In addition, the expression of light sensing proteins, OPN3 and rhodopsin,
was detected. The observed effects were found to be independent of handling effects
such as temperature differences and cytochrome c oxidase dependent ATP increase,
but they were found to be enhanced through the knockout of OPN3. The exact mechanism
of how islets cells sense light and the identity of the photoreceptor remains
unknown.\r\nSummarized two new light-based systems with unique features were established
that enable the activation of proliferative and pro-survival signaling pathways.
While red-light-activated receptor tyrosine kinases open a new avenue for optogenetics
research, by allowing non-invasive control of signaling in vivo, the identified
endogenous light-sensitive mechanism has the potential to be the basis of a gene
therapy-free therapeutical approach for light-based β-cell expansion."
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Eva
full_name: Gschaider-Reichhart, Eva
id: 3FEE232A-F248-11E8-B48F-1D18A9856A87
last_name: Gschaider-Reichhart
orcid: 0000-0002-7218-7738
citation:
ama: Gschaider-Reichhart E. Optical and optogenetic control of proliferation and
survival . 2018. doi:10.15479/AT:ISTA:th_913
apa: Gschaider-Reichhart, E. (2018). Optical and optogenetic control of proliferation
and survival . Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:th_913
chicago: Gschaider-Reichhart, Eva. “Optical and Optogenetic Control of Proliferation
and Survival .” Institute of Science and Technology Austria, 2018. https://doi.org/10.15479/AT:ISTA:th_913.
ieee: E. Gschaider-Reichhart, “Optical and optogenetic control of proliferation
and survival ,” Institute of Science and Technology Austria, 2018.
ista: Gschaider-Reichhart E. 2018. Optical and optogenetic control of proliferation
and survival . Institute of Science and Technology Austria.
mla: Gschaider-Reichhart, Eva. Optical and Optogenetic Control of Proliferation
and Survival . Institute of Science and Technology Austria, 2018, doi:10.15479/AT:ISTA:th_913.
short: E. Gschaider-Reichhart, Optical and Optogenetic Control of Proliferation
and Survival , Institute of Science and Technology Austria, 2018.
date_created: 2018-12-11T11:46:22Z
date_published: 2018-01-08T00:00:00Z
date_updated: 2023-09-22T09:20:10Z
day: '08'
ddc:
- '571'
- '570'
degree_awarded: PhD
department:
- _id: HaJa
doi: 10.15479/AT:ISTA:th_913
file:
- access_level: closed
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date_created: 2019-04-05T09:28:03Z
date_updated: 2020-07-14T12:46:24Z
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has_accepted_license: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: '107'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '7405'
pubrep_id: '913'
related_material:
record:
- id: '1441'
relation: part_of_dissertation
status: public
- id: '1678'
relation: part_of_dissertation
status: public
- id: '2084'
relation: part_of_dissertation
status: public
- id: '1028'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Harald L
full_name: Janovjak, Harald L
id: 33BA6C30-F248-11E8-B48F-1D18A9856A87
last_name: Janovjak
orcid: 0000-0002-8023-9315
title: 'Optical and optogenetic control of proliferation and survival '
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2018'
...
---
_id: '52'
abstract:
- lang: eng
text: In this thesis we will discuss systems of point interacting fermions, their
stability and other spectral properties. Whereas for bosons a point interacting
system is always unstable this ques- tion is more subtle for a gas of two species
of fermions. In particular the answer depends on the mass ratio between these
two species. Most of this work will be focused on the N + M model which consists
of two species of fermions with N, M particles respectively which interact via
point interactions. We will introduce this model using a formal limit and discuss
the N + 1 system in more detail. In particular, we will show that for mass ratios
above a critical one, which does not depend on the particle number, the N + 1
system is stable. In the context of this model we will prove rigorous versions
of Tan relations which relate various quantities of the point-interacting model.
By restricting the N + 1 system to a box we define a finite density model with
point in- teractions. In the context of this system we will discuss the energy
change when introducing a point-interacting impurity into a system of non-interacting
fermions. We will see that this change in energy is bounded independently of the
particle number and in particular the bound only depends on the density and the
scattering length. As another special case of the N + M model we will show stability
of the 2 + 2 model for mass ratios in an interval around one. Further we will
investigate a different model of point interactions which was discussed before
in the literature and which is, contrary to the N + M model, not given by a limiting
procedure but is based on a Dirichlet form. We will show that this system behaves
trivially in the thermodynamic limit, i.e. the free energy per particle is the
same as the one of the non-interacting system.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Thomas
full_name: Moser, Thomas
id: 2B5FC9A4-F248-11E8-B48F-1D18A9856A87
last_name: Moser
citation:
ama: Moser T. Point interactions in systems of fermions. 2018. doi:10.15479/AT:ISTA:th_1043
apa: Moser, T. (2018). Point interactions in systems of fermions. Institute
of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:th_1043
chicago: Moser, Thomas. “Point Interactions in Systems of Fermions.” Institute of
Science and Technology Austria, 2018. https://doi.org/10.15479/AT:ISTA:th_1043.
ieee: T. Moser, “Point interactions in systems of fermions,” Institute of Science
and Technology Austria, 2018.
ista: Moser T. 2018. Point interactions in systems of fermions. Institute of Science
and Technology Austria.
mla: Moser, Thomas. Point Interactions in Systems of Fermions. Institute
of Science and Technology Austria, 2018, doi:10.15479/AT:ISTA:th_1043.
short: T. Moser, Point Interactions in Systems of Fermions, Institute of Science
and Technology Austria, 2018.
date_created: 2018-12-11T11:44:22Z
date_published: 2018-09-04T00:00:00Z
date_updated: 2023-09-27T12:34:14Z
day: '04'
ddc:
- '515'
- '530'
- '519'
degree_awarded: PhD
department:
- _id: RoSe
doi: 10.15479/AT:ISTA:th_1043
file:
- access_level: open_access
checksum: fbd8c747d148b468a21213b7cf175225
content_type: application/pdf
creator: dernst
date_created: 2019-04-09T07:45:38Z
date_updated: 2020-07-14T12:46:37Z
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file_name: 2018_Thesis_Moser.pdf
file_size: 851164
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creator: dernst
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date_updated: 2020-07-14T12:46:37Z
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file_name: 2018_Thesis_Moser_Source.zip
file_size: 1531516
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has_accepted_license: '1'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
page: '115'
project:
- _id: 25C878CE-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P27533_N27
name: Structure of the Excitation Spectrum for Many-Body Quantum Systems
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '8002'
pubrep_id: '1043'
related_material:
record:
- id: '5856'
relation: part_of_dissertation
status: public
- id: '154'
relation: part_of_dissertation
status: public
- id: '1198'
relation: part_of_dissertation
status: public
- id: '741'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Robert
full_name: Seiringer, Robert
id: 4AFD0470-F248-11E8-B48F-1D18A9856A87
last_name: Seiringer
orcid: 0000-0002-6781-0521
title: Point interactions in systems of fermions
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2018'
...
---
_id: '69'
abstract:
- lang: eng
text: 'A qubit, a unit of quantum information, is essentially any quantum mechanical
two-level system which can be coherently controlled. Still, to be used for computation,
it has to fulfill criteria. Qubits, regardless of the system in which they are
realized, suffer from decoherence. This leads to loss of the information stored
in the qubit. The upper bound of the time scale on which decoherence happens is
set by the spin relaxation time. In this thesis I studied a two-level system consisting
of a Zeeman-split hole spin confined in a quantum dot formed in a Ge hut wire.
Such Ge hut wires have emerged as a promising material system for the realization
of spin qubits, due to the combination of two significant properties: long spin
coherence time as expected for group IV semiconductors due to the low hyperfine
interaction and a strong valence band spin-orbit coupling. Here, I present how
to fabricate quantum dot devices suitable for electrical transport measurements.
Coupled quantum dot devices allowed the realization of a charge sensor, which
is electrostatically and tunnel coupled to a quantum dot. By integrating the charge
sensor into a radio-frequency reflectometry setup, I performed for the first time
single-shot readout measurements of hole spins and extracted the hole spin relaxation
times in Ge hut wires.'
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Lada
full_name: Vukušić, Lada
id: 31E9F056-F248-11E8-B48F-1D18A9856A87
last_name: Vukušić
orcid: 0000-0003-2424-8636
citation:
ama: Vukušić L. Charge sensing and spin relaxation times of holes in Ge hut wires.
2018. doi:10.15479/AT:ISTA:TH_1047
apa: Vukušić, L. (2018). Charge sensing and spin relaxation times of holes in
Ge hut wires. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:TH_1047
chicago: Vukušić, Lada. “Charge Sensing and Spin Relaxation Times of Holes in Ge
Hut Wires.” Institute of Science and Technology Austria, 2018. https://doi.org/10.15479/AT:ISTA:TH_1047.
ieee: L. Vukušić, “Charge sensing and spin relaxation times of holes in Ge hut wires,”
Institute of Science and Technology Austria, 2018.
ista: Vukušić L. 2018. Charge sensing and spin relaxation times of holes in Ge hut
wires. Institute of Science and Technology Austria.
mla: Vukušić, Lada. Charge Sensing and Spin Relaxation Times of Holes in Ge Hut
Wires. Institute of Science and Technology Austria, 2018, doi:10.15479/AT:ISTA:TH_1047.
short: L. Vukušić, Charge Sensing and Spin Relaxation Times of Holes in Ge Hut Wires,
Institute of Science and Technology Austria, 2018.
date_created: 2018-12-11T11:44:28Z
date_published: 2018-09-01T00:00:00Z
date_updated: 2023-09-26T15:50:22Z
day: '01'
ddc:
- '530'
- '600'
degree_awarded: PhD
department:
- _id: GeKa
- _id: GradSch
doi: 10.15479/AT:ISTA:TH_1047
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language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
page: '103'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '7985'
pubrep_id: '1047'
related_material:
record:
- id: '23'
relation: part_of_dissertation
status: public
- id: '840'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Georgios
full_name: Katsaros, Georgios
id: 38DB5788-F248-11E8-B48F-1D18A9856A87
last_name: Katsaros
orcid: 0000-0001-8342-202X
title: Charge sensing and spin relaxation times of holes in Ge hut wires
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2018'
...
---
_id: '324'
abstract:
- lang: eng
text: Neuronal networks in the brain consist of two main types of neuron, glutamatergic
principal neurons and GABAergic interneurons. Although these interneurons only
represent 10–20% of the whole population, they mediate feedback and feedforward
inhibition and are involved in the generation of high-frequency network oscillations.
A hallmark functional property of GABAergic interneurons, especially of the parvalbumin‑expressing
(PV+) subtypes, is the speed of signaling at their output synapse across species
and brain regions. Several molecular and subcellular factors may underlie the
submillisecond signaling at GABAergic synapses. Such as the selective use of P/Q
type Ca2+ channels and the tight coupling between Ca2+ channels and Ca2+ sensors
of exocytosis. However, whether the molecular identity of the release sensor contributes
to these signaling properties remains unclear. Besides, these interneurons are
mainly show depression in response to train of stimuli. How could they keep sufficient
release to control the activity of postsynaptic principal neurons during high
network activity, is largely elusive. For my Ph.D. work, we firstly examined the
Ca2+ sensor of exocytosis at the GABAergic basket cell (BC) to Purkinje cell (PC)
synapse in the cerebellum. Immunolabeling suggested that BC terminals selectively
expressed synaptotagmin 2 (Syt2), whereas synaptotagmin 1 (Syt1) was enriched
in excitatory terminals. Genetic elimination of Syt2 reduced action potential-evoked
release to ~10% compared to the wild-type control, identifying Syt2 as the major
Ca2+ sensor at BC‑PC synapses. Differential adenovirus-mediated rescue revealed
Syt2 triggered release with shorter latency and higher temporal precision, and
mediated faster vesicle pool replenishment than Syt1. Furthermore, deletion of
Syt2 severely reduced and delayed disynaptic inhibition following parallel fiber
stimulation. Thus, the selective use of Syt2 as the release sensor at BC–PC synapse
ensures fast feedforward inhibition in cerebellar microcircuits. Additionally,
we tested the function of another synaptotagmin member, Syt7, for inhibitory synaptic
transmission at the BC–PC synapse. Syt7 is thought to be a Ca2+ sensor that mediates
asynchronous transmitter release and facilitation at synapses. However, it is
strongly expressed in fast-spiking, PV+ GABAergic interneurons and the output
synapses of these neurons produce only minimal asynchronous release and show depression
rather than facilitation. How could Syt7, a facilitation sensor, contribute to
the depressed inhibitory synaptic transmission needs to be further investigated
and understood. Our results indicated that at the BC–PC synapse, Syt7 contributes
to asynchronous release, pool replenishment and facilitation. In combination,
these three effects ensure efficient transmitter release during high‑frequency
activity and guarantee frequency independence of inhibition. Taken together, our
results confirmed that Syt2, which has the fastest kinetic properties among all
synaptotagmin members, is mainly used by the inhibitory BC‑PC synapse for synaptic
transmission, contributing to the speed and temporal precision of transmitter
release. Furthermore, we showed that Syt7, another highly expressed synaptotagmin
member in the output synapses of cerebellar BCs, is used for ensuring efficient
inhibitor synaptic transmission during high activity.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Chong
full_name: Chen, Chong
id: 3DFD581A-F248-11E8-B48F-1D18A9856A87
last_name: Chen
citation:
ama: Chen C. Synaptotagmins ensure speed and efficiency of inhibitory neurotransmitter
release. 2018. doi:10.15479/AT:ISTA:th_997
apa: Chen, C. (2018). Synaptotagmins ensure speed and efficiency of inhibitory
neurotransmitter release. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:th_997
chicago: Chen, Chong. “Synaptotagmins Ensure Speed and Efficiency of Inhibitory
Neurotransmitter Release.” Institute of Science and Technology Austria, 2018.
https://doi.org/10.15479/AT:ISTA:th_997.
ieee: C. Chen, “Synaptotagmins ensure speed and efficiency of inhibitory neurotransmitter
release,” Institute of Science and Technology Austria, 2018.
ista: Chen C. 2018. Synaptotagmins ensure speed and efficiency of inhibitory neurotransmitter
release. Institute of Science and Technology Austria.
mla: Chen, Chong. Synaptotagmins Ensure Speed and Efficiency of Inhibitory Neurotransmitter
Release. Institute of Science and Technology Austria, 2018, doi:10.15479/AT:ISTA:th_997.
short: C. Chen, Synaptotagmins Ensure Speed and Efficiency of Inhibitory Neurotransmitter
Release, Institute of Science and Technology Austria, 2018.
date_created: 2018-12-11T11:45:49Z
date_published: 2018-03-01T00:00:00Z
date_updated: 2023-09-27T12:26:03Z
day: '01'
ddc:
- '571'
degree_awarded: PhD
department:
- _id: PeJo
doi: 10.15479/AT:ISTA:th_997
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creator: dernst
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date_updated: 2020-07-14T12:46:04Z
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file_name: 2018_Thesis_chong_source.pages
file_size: 47841940
relation: source_file
file_date_updated: 2020-07-14T12:46:04Z
has_accepted_license: '1'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
page: '110'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '7541'
pubrep_id: '997'
related_material:
record:
- id: '1117'
relation: part_of_dissertation
status: public
- id: '749'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
title: Synaptotagmins ensure speed and efficiency of inhibitory neurotransmitter release
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2018'
...
---
_id: '14306'
abstract:
- lang: eng
text: 'Function and activity of biomolecules often depend on their spatial arrangement.
The method introduced here allows genetically encoding the spatial arrangement
of proteins and DNA. The approach relies on staple proteins that fold double-stranded
DNA into user-defined shapes. This thesis describes the development of staple
proteins based on the DNA recognition of TAL effectors and presents experimentally
derived rules for designing a variety of self-assembling nanoscale shapes featuring
structural motifs such as curvature, vertices, corners, and multilayer helix packing. '
article_processing_charge: No
author:
- first_name: Florian M
full_name: Praetorius, Florian M
id: dfec9381-4341-11ee-8fd8-faa02bba7d62
last_name: Praetorius
citation:
ama: Praetorius FM. Genetically encoding the spatial arrangement of DNA and proteins
in self-assembling nanostructures. 2018.
apa: Praetorius, F. M. (2018). Genetically encoding the spatial arrangement of
DNA and proteins in self-assembling nanostructures. Technische Universität
München.
chicago: Praetorius, Florian M. “Genetically Encoding the Spatial Arrangement of
DNA and Proteins in Self-Assembling Nanostructures.” Technische Universität München,
2018.
ieee: F. M. Praetorius, “Genetically encoding the spatial arrangement of DNA and
proteins in self-assembling nanostructures,” Technische Universität München, 2018.
ista: Praetorius FM. 2018. Genetically encoding the spatial arrangement of DNA and
proteins in self-assembling nanostructures. Technische Universität München.
mla: Praetorius, Florian M. Genetically Encoding the Spatial Arrangement of DNA
and Proteins in Self-Assembling Nanostructures. Technische Universität München,
2018.
short: F.M. Praetorius, Genetically Encoding the Spatial Arrangement of DNA and
Proteins in Self-Assembling Nanostructures, Technische Universität München, 2018.
date_created: 2023-09-06T13:11:22Z
date_published: 2018-01-16T00:00:00Z
date_updated: 2023-11-07T11:43:38Z
day: '16'
degree_awarded: PhD
extern: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://mediatum.ub.tum.de/1398662
month: '01'
oa: 1
oa_version: Published Version
publication_status: published
publisher: Technische Universität München
status: public
supervisor:
- first_name: Hendrik
full_name: Dietz, Hendrik
last_name: Dietz
title: Genetically encoding the spatial arrangement of DNA and proteins in self-assembling
nanostructures
type: dissertation
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2018'
...
---
_id: '278'
abstract:
- lang: eng
text: 'Consortial subscription contracts regulate the digital access to publications
between publishers and scientific libraries. However, since a couple of years
the tendency towards a freely accessible publishing (Open Access) intensifies.
As a consequence of this trend the contractual relationship between licensor and
licensee is gradually changing as well: More and more contracts exercise influence
on open access publishing. The present study attempts to compare Austrian examples
of consortial licence contracts, which include components of open access. It describes
the difference between pure subscription contracts and differing innovative deals
including open access components. Thereby it becomes obvious that for the evaluation
of this licence contracts new methods are needed. An essential new element of
such analyses is the evaluation of the open access publication numbers. So this
study tries to carry out such publication analyses for Austrian open access deals
focusing on quantitative questions: How does the number of publications evolve?
How does the open access share change? Publications reports of the publishers
and database queries from Scopus form the data basis. The analysis of the data
points out that differing approaches of contracts result in highly divergent results:
Particular deals can prioritize a saving in costs or else the increase of the
open access rate. It is to be assumed that within the following years further
numerous open access deals will be negotiated. The finding of this study shall
provide guidance.'
author:
- first_name: Márton
full_name: Villányi, Márton
id: 3FFCCD3A-F248-11E8-B48F-1D18A9856A87
last_name: Villányi
orcid: 0000-0001-8126-0426
citation:
ama: Villányi M. Lizenzverträge mit Open-Access-Komponenten an österreichischen
Bibliotheken. 2018.
apa: Villányi, M. (2018). Lizenzverträge mit Open-Access-Komponenten an österreichischen
Bibliotheken. Universität Wien.
chicago: Villányi, Márton. “Lizenzverträge mit Open-Access-Komponenten an österreichischen
Bibliotheken.” Universität Wien, 2018.
ieee: M. Villányi, “Lizenzverträge mit Open-Access-Komponenten an österreichischen
Bibliotheken,” Universität Wien, 2018.
ista: Villányi M. 2018. Lizenzverträge mit Open-Access-Komponenten an österreichischen
Bibliotheken. Universität Wien.
mla: Villányi, Márton. Lizenzverträge mit Open-Access-Komponenten an österreichischen
Bibliotheken. Universität Wien, 2018.
short: M. Villányi, Lizenzverträge mit Open-Access-Komponenten an österreichischen
Bibliotheken, Universität Wien, 2018.
date_created: 2018-12-11T11:45:34Z
date_published: 2018-04-06T00:00:00Z
date_updated: 2024-02-21T13:44:07Z
day: '06'
department:
- _id: E-Lib
language:
- iso: ger
main_file_link:
- open_access: '1'
url: http://othes.univie.ac.at/51113/
month: '04'
oa: 1
oa_version: Published Version
page: '94'
publication_status: published
publisher: Universität Wien
publist_id: '7624'
related_material:
record:
- id: '5577'
relation: dissertation_contains
status: public
- id: '5574'
relation: dissertation_contains
status: public
- id: '5578'
relation: dissertation_contains
status: public
- id: '5579'
relation: dissertation_contains
status: public
- id: '5576'
relation: dissertation_contains
status: public
- id: '5575'
relation: dissertation_contains
status: public
- id: '5582'
relation: dissertation_contains
status: public
- id: '5581'
relation: dissertation_contains
status: public
- id: '5580'
relation: dissertation_contains
status: public
status: public
supervisor:
- first_name: Brigitte
full_name: Kromp, Brigitte
last_name: Kromp
title: Lizenzverträge mit Open-Access-Komponenten an österreichischen Bibliotheken
type: dissertation
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2018'
...
---
_id: '149'
abstract:
- lang: eng
text: The eigenvalue density of many large random matrices is well approximated
by a deterministic measure, the self-consistent density of states. In the present
work, we show this behaviour for several classes of random matrices. In fact,
we establish that, in each of these classes, the self-consistent density of states
approximates the eigenvalue density of the random matrix on all scales slightly
above the typical eigenvalue spacing. For large classes of random matrices, the
self-consistent density of states exhibits several universal features. We prove
that, under suitable assumptions, random Gram matrices and Hermitian random matrices
with decaying correlations have a 1/3-Hölder continuous self-consistent density
of states ρ on R, which is analytic, where it is positive, and has either a square
root edge or a cubic root cusp, where it vanishes. We, thus, extend the validity
of the corresponding result for Wigner-type matrices from [4, 5, 7]. We show that
ρ is determined as the inverse Stieltjes transform of the normalized trace of
the unique solution m(z) to the Dyson equation −m(z) −1 = z − a + S[m(z)] on C
N×N with the constraint Im m(z) ≥ 0. Here, z lies in the complex upper half-plane,
a is a self-adjoint element of C N×N and S is a positivity-preserving operator
on C N×N encoding the first two moments of the random matrix. In order to analyze
a possible limit of ρ for N → ∞ and address some applications in free probability
theory, we also consider the Dyson equation on infinite dimensional von Neumann
algebras. We present two applications to random matrices. We first establish that,
under certain assumptions, large random matrices with independent entries have
a rotationally symmetric self-consistent density of states which is supported
on a centered disk in C. Moreover, it is infinitely often differentiable apart
from a jump on the boundary of this disk. Second, we show edge universality at
all regular (not necessarily extreme) spectral edges for Hermitian random matrices
with decaying correlations.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Johannes
full_name: Alt, Johannes
id: 36D3D8B6-F248-11E8-B48F-1D18A9856A87
last_name: Alt
citation:
ama: Alt J. Dyson equation and eigenvalue statistics of random matrices. 2018. doi:10.15479/AT:ISTA:TH_1040
apa: Alt, J. (2018). Dyson equation and eigenvalue statistics of random matrices.
Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:TH_1040
chicago: Alt, Johannes. “Dyson Equation and Eigenvalue Statistics of Random Matrices.”
Institute of Science and Technology Austria, 2018. https://doi.org/10.15479/AT:ISTA:TH_1040.
ieee: J. Alt, “Dyson equation and eigenvalue statistics of random matrices,” Institute
of Science and Technology Austria, 2018.
ista: Alt J. 2018. Dyson equation and eigenvalue statistics of random matrices.
Institute of Science and Technology Austria.
mla: Alt, Johannes. Dyson Equation and Eigenvalue Statistics of Random Matrices.
Institute of Science and Technology Austria, 2018, doi:10.15479/AT:ISTA:TH_1040.
short: J. Alt, Dyson Equation and Eigenvalue Statistics of Random Matrices, Institute
of Science and Technology Austria, 2018.
date_created: 2018-12-11T11:44:53Z
date_published: 2018-07-12T00:00:00Z
date_updated: 2024-02-22T14:34:33Z
day: '12'
ddc:
- '515'
- '519'
degree_awarded: PhD
department:
- _id: LaEr
doi: 10.15479/AT:ISTA:TH_1040
ec_funded: 1
file:
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checksum: d4dad55a7513f345706aaaba90cb1bb8
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date_created: 2019-04-08T13:55:20Z
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creator: dernst
date_created: 2019-04-08T13:55:20Z
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file_name: 2018_thesis_Alt_source.zip
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relation: source_file
file_date_updated: 2020-07-14T12:44:57Z
has_accepted_license: '1'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
page: '456'
project:
- _id: 258DCDE6-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '338804'
name: Random matrices, universality and disordered quantum systems
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '7772'
pubrep_id: '1040'
related_material:
record:
- id: '1677'
relation: part_of_dissertation
status: public
- id: '550'
relation: part_of_dissertation
status: public
- id: '6183'
relation: part_of_dissertation
status: public
- id: '566'
relation: part_of_dissertation
status: public
- id: '1010'
relation: part_of_dissertation
status: public
- id: '6240'
relation: part_of_dissertation
status: public
- id: '6184'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: László
full_name: Erdös, László
id: 4DBD5372-F248-11E8-B48F-1D18A9856A87
last_name: Erdös
orcid: 0000-0001-5366-9603
title: Dyson equation and eigenvalue statistics of random matrices
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2018'
...
---
_id: '395'
abstract:
- lang: eng
text: 'Autism spectrum disorders (ASD) are a group of genetic disorders often overlapping
with other neurological conditions. Despite the remarkable number of scientific
breakthroughs of the last 100 years, the treatment of neurodevelopmental disorders
(e.g. autism spectrum disorder, intellectual disability, epilepsy) remains a great
challenge. Recent advancements in geno mics, like whole-exome or whole-genome
sequencing, have enabled scientists to identify numerous mutations underlying
neurodevelopmental disorders. Given the few hundred risk genes that were discovered,
the etiological variability and the heterogeneous phenotypic outcomes, the need
for genotype -along with phenotype- based diagnosis of individual patients becomes
a requisite. Driven by this rationale, in a previous study our group described
mutations, identified via whole - exome sequencing, in the gene BCKDK – encoding
for a key regulator of branched chain amin o acid (BCAA) catabolism - as a cause
of ASD. Following up on the role of BCAAs, in the study described here we show
that the solute carrier transporter 7a5 (SLC7A5), a large neutral amino acid transporter
localized mainly at the blood brain barrier (BBB), has an essential role in maintaining
normal levels of brain BCAAs. In mice, deletion of Slc7a5 from the endothelial
cells of the BBB leads to atypical brain amino acid profile, abnormal mRNA translation
and severe neurolo gical abnormalities. Additionally, deletion of Slc7a5 from
the neural progenitor cell population leads to microcephaly. Interestingly, we
demonstrate that BCAA intracerebroventricular administration ameliorates abnormal
behaviors in adult mutant mice. Furthermore, whole - exome sequencing of patients
diagnosed with neurological dis o r ders helped us identify several patients with
autistic traits, microcephaly and motor delay carrying deleterious homozygous
mutations in the SLC7A5 gene. In conclusion, our data elucidate a neurological
syndrome defined by SLC7A5 mutations and support an essential role for t he BCAA
s in human bra in function. Together with r ecent studies (described in chapter
two) that have successfully made the transition into clinical practice, our findings
on the role of B CAAs might have a crucial impact on the development of novel
individualized therapeutic strategies for ASD. '
acknowledged_ssus:
- _id: PreCl
- _id: EM-Fac
- _id: Bio
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Dora-Clara
full_name: Tarlungeanu, Dora-Clara
id: 2ABCE612-F248-11E8-B48F-1D18A9856A87
last_name: Tarlungeanu
citation:
ama: Tarlungeanu D-C. The branched chain amino acids in autism spectrum disorders
. 2018. doi:10.15479/AT:ISTA:th_992
apa: Tarlungeanu, D.-C. (2018). The branched chain amino acids in autism spectrum
disorders . Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:th_992
chicago: Tarlungeanu, Dora-Clara. “The Branched Chain Amino Acids in Autism Spectrum
Disorders .” Institute of Science and Technology Austria, 2018. https://doi.org/10.15479/AT:ISTA:th_992.
ieee: D.-C. Tarlungeanu, “The branched chain amino acids in autism spectrum disorders
,” Institute of Science and Technology Austria, 2018.
ista: Tarlungeanu D-C. 2018. The branched chain amino acids in autism spectrum disorders
. Institute of Science and Technology Austria.
mla: Tarlungeanu, Dora-Clara. The Branched Chain Amino Acids in Autism Spectrum
Disorders . Institute of Science and Technology Austria, 2018, doi:10.15479/AT:ISTA:th_992.
short: D.-C. Tarlungeanu, The Branched Chain Amino Acids in Autism Spectrum Disorders
, Institute of Science and Technology Austria, 2018.
date_created: 2018-12-11T11:46:14Z
date_published: 2018-03-01T00:00:00Z
date_updated: 2023-09-07T12:38:59Z
day: '01'
ddc:
- '570'
- '616'
degree_awarded: PhD
department:
- _id: GaNo
doi: 10.15479/AT:ISTA:th_992
file:
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checksum: 9f5231c96e0ad945040841a8630232da
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
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date_created: 2019-04-05T09:19:17Z
date_updated: 2021-02-11T23:30:15Z
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date_created: 2019-04-05T09:19:17Z
date_updated: 2021-02-11T11:17:16Z
embargo: 2018-03-15
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language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
page: '88'
project:
- _id: 25473368-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: F03523
name: Transmembrane Transporters in Health and Disease
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '7434'
pubrep_id: '992'
related_material:
record:
- id: '1183'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Gaia
full_name: Novarino, Gaia
id: 3E57A680-F248-11E8-B48F-1D18A9856A87
last_name: Novarino
orcid: 0000-0002-7673-7178
title: 'The branched chain amino acids in autism spectrum disorders '
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2018'
...
---
_id: '51'
abstract:
- lang: eng
text: Asymmetries have long been known about in the central nervous system. From
gross anatomical differences, such as the presence of the parapineal organ in
only one hemisphere of the developing zebrafish, to more subtle differences in
activity between both hemispheres, as seen in freely roaming animals or human
participants under PET and fMRI imaging analysis. The presence of asymmetries
has been demonstrated to have huge behavioural implications, with their disruption
often leading to the generation of neurological disorders, memory problems, changes
in personality, and in an organism's health and well-being. For my Ph.D. work
I aimed to tackle two important avenues of research. The first being the process
of input-side dependency in the hippocampus, with the goal of finding a key gene
responsible for its development (Gene X). The second project was to do with experience-induced
laterality formation in the hippocampus. Specifically, how laterality in the synapse
density of the CA1 stratum radiatum (s.r.) could be induced purely through environmental
enrichment. Through unilateral tracer injections into the CA3, I was able to selectively
measure the properties of synapses within the CA1 and investigate how they differed
based upon which hemisphere the presynaptic neurone originated. Having found the
existence of a previously unreported reversed (left-isomerism) i.v. mutant, through
morpholocal examination of labelled terminals in the CA1 s.r., I aimed to elucidate
a key gene responsible for the process of left or right determination of inputs
to the CA1 s.r.. This work relates to the previous finding of input-side dependent
asymmetry in the wild-type rodent, where the origin of the projecting neurone
to the CA1 will determine the morphology of a synapse, to a greater degree than
the hemisphere in which the projection terminates. Using left- and right-isomerism
i.v. mice, in combination with whole genome sequence analysis, I highlight Ena/VASP-like
(Evl) as a potential target for Gene X. In relation to this topic, I also highlight
my work in the recently published paper of how knockout of PirB can lead to a
lack of input-side dependency in the murine hippocampus. For the second question,
I show that the environmental enrichment paradigm will lead to an asymmetry in
the synapse densities in the hippocampus of mice. I also highlight that the nature
of the enrichment is of less consequence than the process of enrichment itself.
I demonstrate that the CA3 region will dramatically alter its projection targets,
in relation to environmental stimulation, with the asymmetry in synaptic density,
caused by enrichment, relying heavily on commissural fibres. I also highlight
the vital importance of input-side dependent asymmetry, as a necessary component
of experience-dependent laterality formation in the CA1 s.r.. However, my results
suggest that it isn't the only cause, as there appears to be a CA1 dependent mechanism
also at play. Upon further investigation, I highlight the significant, and highly
important, finding that the changes seen in the CA1 s.r. were predominantly caused
through projections from the left-CA3, with the right-CA3 having less involvement
in this mechanism.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Matthew J
full_name: Case, Matthew J
id: 44B7CA5A-F248-11E8-B48F-1D18A9856A87
last_name: Case
citation:
ama: 'Case MJ. From the left to the right: A tale of asymmetries, environments,
and hippocampal development. 2018. doi:10.15479/AT:ISTA:th_1032'
apa: 'Case, M. J. (2018). From the left to the right: A tale of asymmetries,
environments, and hippocampal development. Institute of Science and Technology
Austria. https://doi.org/10.15479/AT:ISTA:th_1032'
chicago: 'Case, Matthew J. “From the Left to the Right: A Tale of Asymmetries, Environments,
and Hippocampal Development.” Institute of Science and Technology Austria, 2018.
https://doi.org/10.15479/AT:ISTA:th_1032.'
ieee: 'M. J. Case, “From the left to the right: A tale of asymmetries, environments,
and hippocampal development,” Institute of Science and Technology Austria, 2018.'
ista: 'Case MJ. 2018. From the left to the right: A tale of asymmetries, environments,
and hippocampal development. Institute of Science and Technology Austria.'
mla: 'Case, Matthew J. From the Left to the Right: A Tale of Asymmetries, Environments,
and Hippocampal Development. Institute of Science and Technology Austria,
2018, doi:10.15479/AT:ISTA:th_1032.'
short: 'M.J. Case, From the Left to the Right: A Tale of Asymmetries, Environments,
and Hippocampal Development, Institute of Science and Technology Austria, 2018.'
date_created: 2018-12-11T11:44:22Z
date_published: 2018-06-27T00:00:00Z
date_updated: 2023-09-07T12:39:22Z
day: '27'
ddc:
- '571'
- '576'
degree_awarded: PhD
department:
- _id: RySh
doi: 10.15479/AT:ISTA:th_1032
file:
- access_level: closed
checksum: dcc7b55619d8509dd62b8e99d6cdee44
content_type: application/msword
creator: dernst
date_created: 2019-04-09T07:16:26Z
date_updated: 2021-02-11T23:30:13Z
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creator: dernst
date_created: 2019-04-09T07:16:23Z
date_updated: 2021-02-11T11:17:14Z
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file_id: '6252'
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file_size: 15193621
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file_date_updated: 2021-02-11T23:30:13Z
has_accepted_license: '1'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
page: '186'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '8003'
pubrep_id: '1032'
related_material:
record:
- id: '682'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
title: 'From the left to the right: A tale of asymmetries, environments, and hippocampal
development'
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2018'
...
---
_id: '10'
abstract:
- lang: eng
text: Genomic imprinting is an epigenetic process that leads to parent of origin-specific
gene expression in a subset of genes. Imprinted genes are essential for brain
development, and deregulation of imprinting is associated with neurodevelopmental
diseases and the pathogenesis of psychiatric disorders. However, the cell-type
specificity of imprinting at single cell resolution, and how imprinting and thus
gene dosage regulates neuronal circuit assembly is still largely unknown. Here,
MADM (Mosaic Analysis with Double Markers) technology was employed to assess genomic
imprinting at single cell level. By visualizing MADM-induced uniparental disomies
(UPDs) in distinct colors at single cell level in genetic mosaic animals, this
experimental paradigm provides a unique quantitative platform to systematically
assay the UPD-mediated imbalances in imprinted gene expression at unprecedented
resolution. An experimental pipeline based on FACS, RNA-seq and bioinformatics
analysis was established and applied to systematically map cell-type-specific
‘imprintomes’ in the mouse brain. The results revealed that parental-specific
expression of imprinted genes per se is rarely cell-type-specific even at the
individual cell level. Conversely, when we extended the comparison to downstream
responses resulting from imbalanced imprinted gene expression, we discovered an
unexpectedly high degree of cell-type specificity. Furthermore, we determined
a novel function of genomic imprinting in cortical astrocyte production and in
olfactory bulb (OB) granule cell generation. These results suggest important functional
implication of genomic imprinting for generating cell-type diversity in the brain.
In addition, MADM provides a powerful tool to study candidate genes by concomitant
genetic manipulation and fluorescent labelling of single cells. MADM-based candidate
gene approach was utilized to identify potential imprinted genes involved in the
generation of cortical astrocytes and OB granule cells. We investigated p57Kip2,
a maternally expressed gene and known cell cycle regulator. Although we found
that p57Kip2 does not play a role in these processes, we detected an unexpected
function of the paternal allele previously thought to be silent. Finally, we took
advantage of a key property of MADM which is to allow unambiguous investigation
of environmental impact on single cells. The experimental pipeline based on FACS
and RNA-seq analysis of MADM-labeled cells was established to probe the functional
differences of single cell loss of gene function compared to global loss of function
on a transcriptional level. With this method, both common and distinct responses
were isolated due to cell-autonomous and non-autonomous effects acting on genotypically
identical cells. As a result, transcriptional changes were identified which result
solely from the surrounding environment. Using the MADM technology to study genomic
imprinting at single cell resolution, we have identified cell-type-specific gene
expression, novel gene function and the impact of environment on single cell transcriptomes.
Together, these provide important insights to the understanding of mechanisms
regulating cell-type specificity and thus diversity in the brain.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Susanne
full_name: Laukoter, Susanne
id: 2D6B7A9A-F248-11E8-B48F-1D18A9856A87
last_name: Laukoter
orcid: 0000-0002-7903-3010
citation:
ama: Laukoter S. Role of genomic imprinting in cerebral cortex development. 2018:1-139.
doi:10.15479/AT:ISTA:th1057
apa: Laukoter, S. (2018). Role of genomic imprinting in cerebral cortex development.
Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:th1057
chicago: Laukoter, Susanne. “Role of Genomic Imprinting in Cerebral Cortex Development.”
Institute of Science and Technology Austria, 2018. https://doi.org/10.15479/AT:ISTA:th1057.
ieee: S. Laukoter, “Role of genomic imprinting in cerebral cortex development,”
Institute of Science and Technology Austria, 2018.
ista: Laukoter S. 2018. Role of genomic imprinting in cerebral cortex development.
Institute of Science and Technology Austria.
mla: Laukoter, Susanne. Role of Genomic Imprinting in Cerebral Cortex Development.
Institute of Science and Technology Austria, 2018, pp. 1–139, doi:10.15479/AT:ISTA:th1057.
short: S. Laukoter, Role of Genomic Imprinting in Cerebral Cortex Development, Institute
of Science and Technology Austria, 2018.
date_created: 2018-12-11T11:44:08Z
date_published: 2018-11-21T00:00:00Z
date_updated: 2023-09-07T12:40:44Z
day: '21'
ddc:
- '570'
degree_awarded: PhD
department:
- _id: SiHi
doi: 10.15479/AT:ISTA:th1057
file:
- access_level: closed
checksum: 41fdbf5fdce312802935d88a8ad9932c
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: dernst
date_created: 2019-05-10T07:47:04Z
date_updated: 2019-11-23T23:30:03Z
embargo_to: open_access
file_id: '6396'
file_name: Thesis_LaukoterSusanne_FINAL.docx
file_size: 17949175
relation: source_file
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content_type: application/pdf
creator: dernst
date_created: 2019-05-10T07:47:04Z
date_updated: 2021-02-11T11:17:16Z
embargo: 2019-11-21
file_id: '6397'
file_name: Thesis_LaukoterSusanne_FINAL.pdf
file_size: 21187245
relation: main_file
file_date_updated: 2021-02-11T11:17:16Z
has_accepted_license: '1'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
page: 1 - 139
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '8046'
pubrep_id: '1057'
status: public
supervisor:
- first_name: Beatriz
full_name: Vicoso, Beatriz
id: 49E1C5C6-F248-11E8-B48F-1D18A9856A87
last_name: Vicoso
orcid: 0000-0002-4579-8306
title: Role of genomic imprinting in cerebral cortex development
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2018'
...
---
_id: '323'
abstract:
- lang: eng
text: 'In the here presented thesis, we explore the role of branched actin networks
in cell migration and antigen presentation, the two most relevant processes in
dendritic cell biology. Branched actin networks construct lamellipodial protrusions
at the leading edge of migrating cells. These are typically seen as adhesive structures,
which mediate force transduction to the extracellular matrix that leads to forward
locomotion. We ablated Arp2/3 nucleation promoting factor WAVE in DCs and found
that the resulting cells lack lamellipodial protrusions. Instead, depending on
the maturation state, one or multiple filopodia were formed. By challenging these
cells in a variety of migration assays we found that lamellipodial protrusions
are dispensable for the locomotion of leukocytes and actually dampen the speed
of migration. However, lamellipodia are critically required to negotiate complex
environments that DCs experience while they travel to the next draining lymph
node. Taken together our results suggest that leukocyte lamellipodia have rather
a sensory- than a force transducing function. Furthermore, we show for the first
time structure and dynamics of dendritic cell F-actin at the immunological synapse
with naïve T cells. Dendritic cell F-actin appears as dynamic foci that are nucleated
by the Arp2/3 complex. WAVE ablated dendritic cells show increased membrane tension,
leading to an altered ultrastructure of the immunological synapse and severe T
cell priming defects. These results point towards a previously unappreciated role
of the cellular mechanics of dendritic cells in T cell activation. Additionally,
we present a novel cell culture based system for the differentiation of dendritic
cells from conditionally immortalized hematopoietic precursors. These precursor
cells are genetically tractable via the CRISPR/Cas9 system while they retain their
ability to differentiate into highly migratory dendritic cells and other immune
cells. This will foster the study of all aspects of dendritic cell biology and
beyond. '
acknowledged_ssus:
- _id: NanoFab
- _id: Bio
- _id: PreCl
- _id: EM-Fac
acknowledgement: "First of all I would like to thank Michael Sixt for giving me the
opportunity to work in \r\nhis group and for his support throughout the years. He
is a truly inspiring person and \r\nthe best boss one can imagine. I would
\ also like to thank all current and past \r\nmembers of the Sixt group for
their help and the great working atmosphere in the lab. \r\nIt is a true privilege
to work with such a bright, funny and friendly group of people and \r\nI’m proud
\ that I could be part of it. Furthermore, I would like to say ‘thank
\ you’ to Daria Siekhaus for all the meetings and discussion we had throughout
the years \r\nand to Federica Benvenuti for being part of my committee.
\ I am also grateful to Jack \r\nMerrin in the nanofabrication facility
\ and all the people working in the bioimaging-\r\n, the electron microscopy-
and the preclinical facilities."
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Alexander F
full_name: Leithner, Alexander F
id: 3B1B77E4-F248-11E8-B48F-1D18A9856A87
last_name: Leithner
orcid: 0000-0002-1073-744X
citation:
ama: Leithner AF. Branched actin networks in dendritic cell biology. 2018. doi:10.15479/AT:ISTA:th_998
apa: Leithner, A. F. (2018). Branched actin networks in dendritic cell biology.
Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:th_998
chicago: Leithner, Alexander F. “Branched Actin Networks in Dendritic Cell Biology.”
Institute of Science and Technology Austria, 2018. https://doi.org/10.15479/AT:ISTA:th_998.
ieee: A. F. Leithner, “Branched actin networks in dendritic cell biology,” Institute
of Science and Technology Austria, 2018.
ista: Leithner AF. 2018. Branched actin networks in dendritic cell biology. Institute
of Science and Technology Austria.
mla: Leithner, Alexander F. Branched Actin Networks in Dendritic Cell Biology.
Institute of Science and Technology Austria, 2018, doi:10.15479/AT:ISTA:th_998.
short: A.F. Leithner, Branched Actin Networks in Dendritic Cell Biology, Institute
of Science and Technology Austria, 2018.
date_created: 2018-12-11T11:45:49Z
date_published: 2018-04-12T00:00:00Z
date_updated: 2023-09-07T12:39:44Z
day: '12'
ddc:
- '571'
- '599'
- '610'
degree_awarded: PhD
department:
- _id: MiSi
doi: 10.15479/AT:ISTA:th_998
file:
- access_level: closed
checksum: d5e3edbac548c26c1fa43a4b37a54a4c
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: dernst
date_created: 2019-04-05T09:23:11Z
date_updated: 2021-02-11T23:30:17Z
embargo_to: open_access
file_id: '6219'
file_name: PhD_thesis_AlexLeithner_final_version.docx
file_size: 29027671
relation: source_file
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checksum: 071f7476db29e41146824ebd0697cb10
content_type: application/pdf
creator: dernst
date_created: 2019-04-05T09:23:11Z
date_updated: 2021-02-11T11:17:16Z
embargo: 2019-04-15
file_id: '6220'
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file_size: 66045341
relation: main_file
file_date_updated: 2021-02-11T23:30:17Z
has_accepted_license: '1'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
page: '99'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '7542'
pubrep_id: '998'
related_material:
record:
- id: '1321'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
title: Branched actin networks in dendritic cell biology
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2018'
...
---
_id: '539'
abstract:
- lang: eng
text: The whole life cycle of plants as well as their responses to environmental
stimuli is governed by a complex network of hormonal regulations. A number of
studies have demonstrated an essential role of both auxin and cytokinin in the
regulation of many aspects of plant growth and development including embryogenesis,
postembryonic organogenic processes such as root, and shoot branching, root and
shoot apical meristem activity and phyllotaxis. Over the last decades essential
knowledge on the key molecular factors and pathways that spatio-temporally define
auxin and cytokinin activities in the plant body has accumulated. However, how
both hormonal pathways are interconnected by a complex network of interactions
and feedback circuits that determines the final outcome of the individual hormone
actions is still largely unknown. Root system architecture establishment and in
particular formation of lateral organs is prime example of developmental process
at whose regulation both auxin and cytokinin pathways converge. To dissect convergence
points and pathways that tightly balance auxin - cytokinin antagonistic activities
that determine the root branching pattern transcriptome profiling was applied.
Genome wide expression analyses of the xylem pole pericycle, a tissue giving rise
to lateral roots, led to identification of genes that are highly responsive to
combinatorial auxin and cytokinin treatments and play an essential function in
the auxin-cytokinin regulated root branching. SYNERGISTIC AUXIN CYTOKININ 1 (SYAC1)
gene, which encodes for a protein of unknown function, was detected among the
top candidate genes of which expression was synergistically up-regulated by simultaneous
hormonal treatment. Plants with modulated SYAC1 activity exhibit severe defects
in the root system establishment and attenuate developmental responses to both
auxin and cytokinin. To explore the biological function of the SYAC1, we employed
different strategies including expression pattern analysis, subcellular localization
and phenotypic analyses of the syac1 loss-of-function and gain-of-function transgenic
lines along with the identification of the SYAC1 interaction partners. Detailed
functional characterization revealed that SYAC1 acts as a developmentally specific
regulator of the secretory pathway to control deposition of cell wall components
and thereby rapidly fine tune elongation growth.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Andrej
full_name: Hurny, Andrej
id: 4DC4AF46-F248-11E8-B48F-1D18A9856A87
last_name: Hurny
orcid: 0000-0003-3638-1426
citation:
ama: Hurny A. Identification and characterization of novel auxin-cytokinin cross-talk
components. 2018. doi:10.15479/AT:ISTA:th_930
apa: Hurny, A. (2018). Identification and characterization of novel auxin-cytokinin
cross-talk components. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:th_930
chicago: Hurny, Andrej. “Identification and Characterization of Novel Auxin-Cytokinin
Cross-Talk Components.” Institute of Science and Technology Austria, 2018. https://doi.org/10.15479/AT:ISTA:th_930.
ieee: A. Hurny, “Identification and characterization of novel auxin-cytokinin cross-talk
components,” Institute of Science and Technology Austria, 2018.
ista: Hurny A. 2018. Identification and characterization of novel auxin-cytokinin
cross-talk components. Institute of Science and Technology Austria.
mla: Hurny, Andrej. Identification and Characterization of Novel Auxin-Cytokinin
Cross-Talk Components. Institute of Science and Technology Austria, 2018,
doi:10.15479/AT:ISTA:th_930.
short: A. Hurny, Identification and Characterization of Novel Auxin-Cytokinin Cross-Talk
Components, Institute of Science and Technology Austria, 2018.
date_created: 2018-12-11T11:47:03Z
date_published: 2018-01-01T00:00:00Z
date_updated: 2023-09-07T12:41:06Z
day: '01'
ddc:
- '570'
degree_awarded: PhD
department:
- _id: EvBe
doi: 10.15479/AT:ISTA:th_930
file:
- access_level: closed
checksum: 0c9d6d1c80d9857e6e545213467bbcb2
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: dernst
date_created: 2019-04-05T09:37:56Z
date_updated: 2020-12-02T23:30:08Z
embargo_to: open_access
file_id: '6226'
file_name: 2018_Hurny_thesis_source.docx
file_size: 28112114
relation: source_file
- access_level: open_access
checksum: ecbe481a1413d270bd501b872c7ed54f
content_type: application/pdf
creator: dernst
date_created: 2019-04-05T09:37:55Z
date_updated: 2020-12-02T09:52:16Z
embargo: 2019-07-10
file_id: '6227'
file_name: 2018_Hurny_thesis.pdf
file_size: 12524427
relation: main_file
file_date_updated: 2020-12-02T23:30:08Z
has_accepted_license: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: '147'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '7277'
pubrep_id: '930'
related_material:
record:
- id: '1024'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
title: Identification and characterization of novel auxin-cytokinin cross-talk components
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2018'
...
---
_id: '48'
abstract:
- lang: eng
text: 'The hippocampus is a key brain region for spatial memory and navigation and
is needed at all stages of memory, including encoding, consolidation, and recall.
Hippocampal place cells selectively discharge at specific locations of the environment
to form a cognitive map of the space. During the rest period and sleep following
spatial navigation and/or learning, the waking activity of the place cells is
reactivated within high synchrony events. This reactivation is thought to be important
for memory consolidation and stabilization of the spatial representations. The
aim of my thesis was to directly test whether the reactivation content encoded
in firing patterns of place cells is important for consolidation of spatial memories.
In particular, I aimed to test whether, in cases when multiple spatial memory
traces are acquired during learning, the specific disruption of the reactivation
of a subset of these memories leads to the selective disruption of the corresponding
memory traces or through memory interference the other learned memories are disrupted
as well. In this thesis, using a modified cheeseboard paradigm and a closed-loop
recording setup with feedback optogenetic stimulation, I examined how the disruption
of the reactivation of specific spiking patterns affects consolidation of the
corresponding memory traces. To obtain multiple distinctive memories, animals
had to perform a spatial task in two distinct cheeseboard environments and the
reactivation of spiking patterns associated with one of the environments (target)
was disrupted after learning during four hours rest period using a real-time decoding
method. This real-time decoding method was capable of selectively affecting the
firing rates and cofiring correlations of the target environment-encoding cells.
The selective disruption led to behavioural impairment in the memory tests after
the rest periods in the target environment but not in the other undisrupted control
environment. In addition, the map of the target environment was less stable in
the impaired memory tests compared to the learning session before than the map
of the control environment. However, when the animal relearned the task, the same
map recurred in the target environment that was present during learning before
the disruption. Altogether my work demonstrated that the reactivation content
is important: assembly-related disruption of reactivation can lead to a selective
memory impairment and deficiency in map stability. These findings indeed suggest
that reactivated assembly patterns reflect processes associated with the consolidation
of memory traces. '
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Igor
full_name: Gridchyn, Igor
id: 4B60654C-F248-11E8-B48F-1D18A9856A87
last_name: Gridchyn
orcid: 0000-0002-1807-1929
citation:
ama: Gridchyn I. Reactivation content is important for consolidation of spatial
memory. 2018. doi:10.15479/AT:ISTA:th_1042
apa: Gridchyn, I. (2018). Reactivation content is important for consolidation
of spatial memory. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:th_1042
chicago: Gridchyn, Igor. “Reactivation Content Is Important for Consolidation of
Spatial Memory.” Institute of Science and Technology Austria, 2018. https://doi.org/10.15479/AT:ISTA:th_1042.
ieee: I. Gridchyn, “Reactivation content is important for consolidation of spatial
memory,” Institute of Science and Technology Austria, 2018.
ista: Gridchyn I. 2018. Reactivation content is important for consolidation of spatial
memory. Institute of Science and Technology Austria.
mla: Gridchyn, Igor. Reactivation Content Is Important for Consolidation of Spatial
Memory. Institute of Science and Technology Austria, 2018, doi:10.15479/AT:ISTA:th_1042.
short: I. Gridchyn, Reactivation Content Is Important for Consolidation of Spatial
Memory, Institute of Science and Technology Austria, 2018.
date_created: 2018-12-11T11:44:21Z
date_published: 2018-08-27T00:00:00Z
date_updated: 2023-09-07T12:42:44Z
day: '27'
ddc:
- '573'
degree_awarded: PhD
department:
- _id: JoCs
doi: 10.15479/AT:ISTA:th_1042
file:
- access_level: closed
checksum: 7db4415e435590fa33542c7b0a0321d7
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: dernst
date_created: 2019-04-08T13:36:01Z
date_updated: 2021-02-11T23:30:22Z
embargo_to: open_access
file_id: '6236'
file_name: 2018_Thesis_Gridchyn_source.docx
file_size: 7666687
relation: source_file
- access_level: open_access
checksum: f96f3fe8979f7b1e6db6acaca962b10c
content_type: application/pdf
creator: dernst
date_created: 2019-04-08T13:36:01Z
date_updated: 2021-02-11T11:17:18Z
embargo: 2019-08-29
file_id: '6237'
file_name: 2018_Thesis_Gridchyn.pdf
file_size: 6034153
relation: main_file
file_date_updated: 2021-02-11T23:30:22Z
has_accepted_license: '1'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
page: '104'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '8006'
pubrep_id: '1042'
status: public
supervisor:
- first_name: Jozsef L
full_name: Csicsvari, Jozsef L
id: 3FA14672-F248-11E8-B48F-1D18A9856A87
last_name: Csicsvari
orcid: 0000-0002-5193-4036
title: Reactivation content is important for consolidation of spatial memory
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2018'
...
---
_id: '9'
abstract:
- lang: eng
text: 'Immune cells migrating to the sites of infection navigate through diverse
tissue architectures and switch their migratory mechanisms upon demand. However,
little is known about systemic regulators that could allow the acquisition of
these mechanisms. We performed a genetic screen in Drosophila melanogaster to
identify regulators of germband invasion by embryonic macrophages into the confined
space between the ectoderm and mesoderm. We have found that bZIP circadian transcription
factors (TFs) Kayak (dFos) and Vrille (dNFIL3) have opposite effects on macrophage
germband infiltration: Kayak facilitated and Vrille inhibited it. These TFs are
enriched in the macrophages during migration and genetically interact to control
it. Kayak sets a less coordinated mode of migration of the macrophage group and
increases the probability and length of Levy walks. Intriguingly, the motility
of kayak mutant macrophages was also strongly affected during initial germband
invasion but not along another less confined route. Inhibiting Rho1 signaling
within the tail ectoderm partially rescued the Kayak mutant phenotype, strongly
suggesting that migrating macrophages have to overcome a barrier imposed by the
stiffness of the ectoderm. Also, Kayak appeared to be important for the maintenance
of the round cell shape and the rear edge translocation of the macrophages invading
the germband. Complementary to this, the cortical actin cytoskeleton of Kayak-
deficient macrophages was strongly affected. RNA sequencing revealed the filamin
Cheerio and tetraspanin TM4SF to be downstream of Kayak. Chromatin immunoprecipitation
and immunostaining revealed that the formin Diaphanous is another downstream target
of Kayak. Immunostaining revealed that the formin Diaphanous is another downstream
target of Kayak. Indeed, Cheerio, TM4SF and Diaphanous are required within macrophages
for germband invasion, and expression of constitutively active Diaphanous in macrophages
was able to rescue the kayak mutant phenotype. Moreover, Cher and Diaphanous are
also reduced in the macrophages overexpressing Vrille. We hypothesize that Kayak,
through its targets, increases actin polymerization and cortical tension in macrophages
and thus allows extra force generation necessary for macrophage dissemination
and migration through confined stiff tissues, while Vrille counterbalances it.'
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Vera
full_name: Belyaeva, Vera
id: 47F080FE-F248-11E8-B48F-1D18A9856A87
last_name: Belyaeva
citation:
ama: Belyaeva V. Transcriptional regulation of macrophage migration in the Drosophila
melanogaster embryo . 2018. doi:10.15479/AT:ISTA:th1064
apa: Belyaeva, V. (2018). Transcriptional regulation of macrophage migration
in the Drosophila melanogaster embryo . Institute of Science and Technology
Austria. https://doi.org/10.15479/AT:ISTA:th1064
chicago: Belyaeva, Vera. “Transcriptional Regulation of Macrophage Migration in
the Drosophila Melanogaster Embryo .” Institute of Science and Technology Austria,
2018. https://doi.org/10.15479/AT:ISTA:th1064.
ieee: V. Belyaeva, “Transcriptional regulation of macrophage migration in the Drosophila
melanogaster embryo ,” Institute of Science and Technology Austria, 2018.
ista: Belyaeva V. 2018. Transcriptional regulation of macrophage migration in the
Drosophila melanogaster embryo . Institute of Science and Technology Austria.
mla: Belyaeva, Vera. Transcriptional Regulation of Macrophage Migration in the
Drosophila Melanogaster Embryo . Institute of Science and Technology Austria,
2018, doi:10.15479/AT:ISTA:th1064.
short: V. Belyaeva, Transcriptional Regulation of Macrophage Migration in the Drosophila
Melanogaster Embryo , Institute of Science and Technology Austria, 2018.
date_created: 2018-12-11T11:44:08Z
date_published: 2018-07-01T00:00:00Z
date_updated: 2023-09-07T12:43:10Z
day: '01'
ddc:
- '570'
degree_awarded: PhD
department:
- _id: DaSi
doi: 10.15479/AT:ISTA:th1064
file:
- access_level: closed
checksum: d27b2465cb70d0c9678a0381b9b6ced1
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: dernst
date_created: 2019-04-08T14:13:12Z
date_updated: 2020-07-14T12:48:14Z
embargo_to: open_access
file_id: '6243'
file_name: 2018_Thesis_Belyaeva_source.docx
file_size: 102737483
relation: source_file
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checksum: a2939b61bde2de7b8ced77bbae0eaaed
content_type: application/pdf
creator: dernst
date_created: 2019-04-08T14:14:08Z
date_updated: 2021-02-11T11:17:16Z
embargo: 2019-11-19
file_id: '6244'
file_name: 2018_Thesis_Belyaeva.pdf
file_size: 88077843
relation: main_file
file_date_updated: 2021-02-11T11:17:16Z
has_accepted_license: '1'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
page: '96'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '8047'
pubrep_id: '1064'
status: public
supervisor:
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
title: 'Transcriptional regulation of macrophage migration in the Drosophila melanogaster
embryo '
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2018'
...
---
_id: '6266'
abstract:
- lang: eng
text: 'A major challenge in neuroscience research is to dissect the circuits that
orchestrate behavior in health and disease. Proteins from a wide range of non-mammalian
species, such as microbial opsins, have been successfully transplanted to specific
neuronal targets to override their natural communication patterns. The goal of
our work is to manipulate synaptic communication in a manner that closely incorporates
the functional intricacies of synapses by preserving temporal encoding (i.e. the
firing pattern of the presynaptic neuron) and connectivity (i.e. target specific
synapses rather than specific neurons). Our strategy to achieve this goal builds
on the use of non-mammalian transplants to create a synthetic synapse. The mode
of modulation comes from pre-synaptic uptake of a synthetic neurotransmitter (SN)
into synaptic vesicles by means of a genetically targeted transporter selective
for the SN. Upon natural vesicular release, exposure of the SN to the synaptic
cleft will modify the post-synaptic potential through an orthogonal ligand gated
ion channel. To achieve this goal we have functionally characterized a mixed cationic
methionine-gated ion channel from Arabidopsis thaliana, designed a method to functionally
characterize a synthetic transporter in isolated synaptic vesicles without the
need for transgenic animals, identified and extracted multiple prokaryotic uptake
systems that are substrate specific for methionine (Met), and established a primary/cell
line co-culture system that would allow future combinatorial testing of this orthogonal
transmitter-transporter-channel trifecta. Synthetic synapses will provide a unique
opportunity to manipulate synaptic communication while maintaining the electrophysiological
integrity of the pre-synaptic cell. In this way, information may be preserved
that was generated in upstream circuits and that could be essential for concerted
function and information processing. '
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Catherine
full_name: Mckenzie, Catherine
id: 3EEDE19A-F248-11E8-B48F-1D18A9856A87
last_name: Mckenzie
citation:
ama: Mckenzie C. Design and characterization of methods and biological components
to realize synthetic neurotransmission . 2018. doi:10.15479/at:ista:th_1055
apa: Mckenzie, C. (2018). Design and characterization of methods and biological
components to realize synthetic neurotransmission . Institute of Science and
Technology Austria. https://doi.org/10.15479/at:ista:th_1055
chicago: Mckenzie, Catherine. “Design and Characterization of Methods and Biological
Components to Realize Synthetic Neurotransmission .” Institute of Science and
Technology Austria, 2018. https://doi.org/10.15479/at:ista:th_1055.
ieee: C. Mckenzie, “Design and characterization of methods and biological components
to realize synthetic neurotransmission ,” Institute of Science and Technology
Austria, 2018.
ista: Mckenzie C. 2018. Design and characterization of methods and biological components
to realize synthetic neurotransmission . Institute of Science and Technology Austria.
mla: Mckenzie, Catherine. Design and Characterization of Methods and Biological
Components to Realize Synthetic Neurotransmission . Institute of Science and
Technology Austria, 2018, doi:10.15479/at:ista:th_1055.
short: C. Mckenzie, Design and Characterization of Methods and Biological Components
to Realize Synthetic Neurotransmission , Institute of Science and Technology Austria,
2018.
date_created: 2019-04-09T14:13:39Z
date_published: 2018-10-31T00:00:00Z
date_updated: 2023-09-07T13:02:37Z
day: '31'
ddc:
- '571'
- '573'
degree_awarded: PhD
department:
- _id: HaJa
doi: 10.15479/at:ista:th_1055
file:
- access_level: open_access
checksum: 9d2c2dca04b00e485470c28b262af59a
content_type: application/pdf
creator: dernst
date_created: 2019-04-09T14:12:40Z
date_updated: 2021-02-11T11:17:16Z
embargo: 2019-11-24
file_id: '6267'
file_name: 2018_Thesis_McKenzie.pdf
file_size: 4906420
relation: main_file
- access_level: closed
checksum: 50b58c272899601bc6fd9642c4dc97f1
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: dernst
date_created: 2019-04-09T14:12:40Z
date_updated: 2020-07-14T12:47:25Z
embargo_to: open_access
file_id: '6268'
file_name: 2018_Thesis_McKenzie_source.docx
file_size: 5053545
relation: source_file
file_date_updated: 2021-02-11T11:17:16Z
has_accepted_license: '1'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
page: '95'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
pubrep_id: '1055'
related_material:
record:
- id: '7132'
relation: new_edition
status: public
status: public
supervisor:
- first_name: Harald L
full_name: Janovjak, Harald L
id: 33BA6C30-F248-11E8-B48F-1D18A9856A87
last_name: Janovjak
orcid: 0000-0002-8023-9315
title: 'Design and characterization of methods and biological components to realize
synthetic neurotransmission '
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2018'
...
---
_id: '50'
abstract:
- lang: eng
text: The Wnt/planar cell polarity (Wnt/PCP) pathway determines planar polarity
of epithelial cells in both vertebrates and invertebrates. The role that Wnt/PCP
signaling plays in mesenchymal contexts, however, is only poorly understood. While
previous studies have demonstrated the capacity of Wnt/PCP signaling to polarize
and guide directed migration of mesenchymal cells, it remains unclear whether
endogenous Wnt/PCP signaling performs these functions instructively, as it does
in epithelial cells. Here we developed a light-switchable version of the Wnt/PCP
receptor Frizzled 7 (Fz7) to unambiguously distinguish between an instructive
and a permissive role of Wnt/PCP signaling for the directional collective migration
of mesendoderm progenitor cells during zebrafish gastrulation. We show that prechordal
plate (ppl) cell migration is defective in maternal-zygotic fz7a and fz7b (MZ
fz7a,b) double mutant embryos, and that Fz7 functions cell-autonomously in this
process by promoting ppl cell protrusion formation and directed migration. We
further show that local activation of Fz7 can direct ppl cell migration both in
vitro and in vivo. Surprisingly, however, uniform Fz7 activation is sufficient
to fully rescue the ppl cell migration defect in MZ fz7a,b mutant embryos, indicating
that Wnt/PCP signaling functions permissively rather than instructively in directed
mesendoderm cell migration during zebrafish gastrulation.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Daniel
full_name: Capek, Daniel
id: 31C42484-F248-11E8-B48F-1D18A9856A87
last_name: Capek
orcid: 0000-0001-5199-9940
citation:
ama: Capek D. Optogenetic Frizzled 7 reveals a permissive function of Wnt/PCP signaling
in directed mesenchymal cell migration. 2018. doi:10.15479/AT:ISTA:TH_1031
apa: Capek, D. (2018). Optogenetic Frizzled 7 reveals a permissive function of
Wnt/PCP signaling in directed mesenchymal cell migration. Institute of Science
and Technology Austria. https://doi.org/10.15479/AT:ISTA:TH_1031
chicago: Capek, Daniel. “Optogenetic Frizzled 7 Reveals a Permissive Function of
Wnt/PCP Signaling in Directed Mesenchymal Cell Migration.” Institute of Science
and Technology Austria, 2018. https://doi.org/10.15479/AT:ISTA:TH_1031.
ieee: D. Capek, “Optogenetic Frizzled 7 reveals a permissive function of Wnt/PCP
signaling in directed mesenchymal cell migration,” Institute of Science and Technology
Austria, 2018.
ista: Capek D. 2018. Optogenetic Frizzled 7 reveals a permissive function of Wnt/PCP
signaling in directed mesenchymal cell migration. Institute of Science and Technology
Austria.
mla: Capek, Daniel. Optogenetic Frizzled 7 Reveals a Permissive Function of Wnt/PCP
Signaling in Directed Mesenchymal Cell Migration. Institute of Science and
Technology Austria, 2018, doi:10.15479/AT:ISTA:TH_1031.
short: D. Capek, Optogenetic Frizzled 7 Reveals a Permissive Function of Wnt/PCP
Signaling in Directed Mesenchymal Cell Migration, Institute of Science and Technology
Austria, 2018.
date_created: 2018-12-11T11:44:21Z
date_published: 2018-06-22T00:00:00Z
date_updated: 2023-09-07T12:48:16Z
day: '22'
ddc:
- '570'
- '591'
- '596'
degree_awarded: PhD
department:
- _id: CaHe
doi: 10.15479/AT:ISTA:TH_1031
file:
- access_level: open_access
checksum: d3eca3dcacb67bffdde6e6609c31cdd0
content_type: application/pdf
creator: dernst
date_created: 2019-04-08T13:42:26Z
date_updated: 2021-02-11T11:17:17Z
embargo: 2019-06-25
file_id: '6238'
file_name: 2018_Thesis_Capek.pdf
file_size: 31576521
relation: main_file
- access_level: closed
checksum: 876deb14067e638aba65d209668bd821
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: dernst
date_created: 2019-04-08T13:42:27Z
date_updated: 2021-02-11T23:30:21Z
embargo_to: open_access
file_id: '6239'
file_name: 2018_Thesis_Capek_source.docx
file_size: 38992956
relation: source_file
file_date_updated: 2021-02-11T23:30:21Z
has_accepted_license: '1'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
page: '95'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '8004'
pubrep_id: '1031'
related_material:
record:
- id: '1100'
relation: part_of_dissertation
status: public
- id: '661'
relation: part_of_dissertation
status: public
- id: '676'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
title: Optogenetic Frizzled 7 reveals a permissive function of Wnt/PCP signaling in
directed mesenchymal cell migration
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2018'
...
---
_id: '26'
abstract:
- lang: eng
text: Expression of genes is a fundamental molecular phenotype that is subject to
evolution by different types of mutations. Both the rate and the effect of mutations
may depend on the DNA sequence context of a particular gene or a particular promoter
sequence. In this thesis I investigate the nature of this dependence using simple
genetic systems in Escherichia coli. With these systems I explore the evolution
of constitutive gene expression from random starting sequences at different loci
on the chromosome and at different locations in sequence space. First, I dissect
chromosomal neighborhood effects that underlie locus-dependent differences in
the potential of a gene under selection to become more highly expressed. Next,
I find that the effects of point mutations in promoter sequences are dependent
on sequence context, and that an existing energy matrix model performs poorly
in predicting relative expression of unrelated sequences. Finally, I show that
a substantial fraction of random sequences contain functional promoters and I
present an extended thermodynamic model that predicts promoter strength in full
sequence space. Taken together, these results provide new insights and guides
on how to integrate information on sequence context to improve our qualitative
and quantitative understanding of bacterial gene expression, with implications
for rapid evolution of drug resistance, de novo evolution of genes, and horizontal
gene transfer.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Magdalena
full_name: Steinrück, Magdalena
id: 2C023F40-F248-11E8-B48F-1D18A9856A87
last_name: Steinrück
orcid: 0000-0003-1229-9719
citation:
ama: Steinrück M. The influence of sequence context on the evolution of bacterial
gene expression. 2018. doi:10.15479/AT:ISTA:th1059
apa: Steinrück, M. (2018). The influence of sequence context on the evolution
of bacterial gene expression. Institute of Science and Technology Austria.
https://doi.org/10.15479/AT:ISTA:th1059
chicago: Steinrück, Magdalena. “The Influence of Sequence Context on the Evolution
of Bacterial Gene Expression.” Institute of Science and Technology Austria, 2018.
https://doi.org/10.15479/AT:ISTA:th1059.
ieee: M. Steinrück, “The influence of sequence context on the evolution of bacterial
gene expression,” Institute of Science and Technology Austria, 2018.
ista: Steinrück M. 2018. The influence of sequence context on the evolution of bacterial
gene expression. Institute of Science and Technology Austria.
mla: Steinrück, Magdalena. The Influence of Sequence Context on the Evolution
of Bacterial Gene Expression. Institute of Science and Technology Austria,
2018, doi:10.15479/AT:ISTA:th1059.
short: M. Steinrück, The Influence of Sequence Context on the Evolution of Bacterial
Gene Expression, Institute of Science and Technology Austria, 2018.
date_created: 2018-12-11T11:44:14Z
date_published: 2018-10-30T00:00:00Z
date_updated: 2023-09-07T12:48:43Z
day: '30'
ddc:
- '576'
- '579'
degree_awarded: PhD
department:
- _id: CaGu
doi: 10.15479/AT:ISTA:th1059
file:
- access_level: closed
checksum: 413cbce1cd1debeae3abe2a25dbc70d1
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: dernst
date_created: 2019-02-08T10:51:22Z
date_updated: 2020-07-14T12:45:43Z
embargo_to: open_access
file_id: '5941'
file_name: Thesis_Steinrueck_final.docx
file_size: 9190845
relation: source_file
- access_level: open_access
checksum: 3def8b7854c8b42d643597ce0215efac
content_type: application/pdf
creator: dernst
date_created: 2019-02-08T10:51:22Z
date_updated: 2021-02-11T11:17:14Z
embargo: 2019-11-02
file_id: '5942'
file_name: Thesis_Steinrueck_final.pdf
file_size: 7521973
relation: main_file
file_date_updated: 2021-02-11T11:17:14Z
has_accepted_license: '1'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
page: '109'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '8029'
pubrep_id: '1059'
related_material:
record:
- id: '704'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Calin C
full_name: Guet, Calin C
id: 47F8433E-F248-11E8-B48F-1D18A9856A87
last_name: Guet
orcid: 0000-0001-6220-2052
title: The influence of sequence context on the evolution of bacterial gene expression
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2018'
...
---
_id: '6263'
abstract:
- lang: eng
text: 'Antibiotic resistance can emerge spontaneously through genomic mutation and render
treatment ineffective. To counteract this process, in addition to the discovery and
description of resistance mechanisms,a deeper understanding of resistanceevolvabilityand
its determinantsis needed. To address this challenge, this thesisuncoversnew genetic
determinants of resistance evolvability using a customized robotic setup,
exploressystematic ways in which resistance evolution is perturbed due to
dose-responsecharacteristics of drugs and mutation rate differences,and mathematically investigates
the evolutionary fate of one specific type of evolvability modifier -a stress-induced
mutagenesis allele.We find severalgenes which strongly inhibit or potentiate resistance evolution. In order
to identify them, we first developedan automated high-throughput feedback-controlled
protocol whichkeeps the population size and selection pressure approximately constant
for hundreds of cultures by dynamically re-diluting the cultures and adjusting the antibiotic
concentration. We implementedthis protocol on a customized liquid handling robot and
propagated 100 different gene deletion strains of Escherichia coliin triplicate for over 100
generations in tetracycline and in chloramphenicol, and comparedtheir adaptation rates.We find a diminishing returns pattern, where initially sensitive strains adapted more
compared to less sensitive ones. Our data uncover that deletions of certain genes
which do not affect mutation rate,including efflux pump components, a chaperone and
severalstructural and regulatory genes can strongly and reproducibly alterresistance evolution.
Sequencing analysis of evolved populations indicates that epistasis with resistance
mutations is the most likelyexplanation. This work could inspire treatment strategies in
which targeted inhibitors of evolvability mechanisms will be given alongside antibiotics to
slow down resistance evolution and extend theefficacy of antibiotics.We implemented astochasticpopulation genetics model,
toverifyways in which general properties, namely, dose-response characteristics of drugs and mutation rates, influence
evolutionary dynamics. In particular, under the exposure to antibiotics with shallow dose-response curves,bacteria have narrower distributions of fitness effects of new mutations.
We show that in silicothis also leads to slower resistance evolution. We
see and confirm with experiments that increased mutation rates, apart from speeding
up evolution, also leadto high reproducibility of phenotypic adaptation in a context
of continually strong selection pressure.Knowledge of these patterns can aid in predicting the dynamics of antibiotic
resistance evolutionand adapting treatment schemes accordingly.Focusing on a previously described type of evolvability modifier
–a stress-induced mutagenesis allele –we find conditions under which it can persist in a population under
periodic selectionakin to clinical treatment. We set up a deterministic
infinite populationcontinuous time model tracking the frequencies of a mutator and resistance allele and
evaluate various treatment schemes in how well they maintain a stress-induced
mutator allele. In particular,a high diversity of stresses is crucial for the persistence
of the mutator allele. This leads to a general trade-off where exactly those
diversifying treatment schemes which are likely to decrease levels of resistance could lead to stronger selection of highly
evolvable genotypes.In the long run, this work will lead to a deeper understanding of the genetic and cellular
mechanisms involved in antibiotic resistance evolution and could inspire new strategies
for slowing down its rate. '
acknowledged_ssus:
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- _id: LifeSc
alternative_title:
- ISTA Thesis
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author:
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full_name: Lukacisinova, Marta
id: 4342E402-F248-11E8-B48F-1D18A9856A87
last_name: Lukacisinova
orcid: 0000-0002-2519-8004
citation:
ama: Lukacisinova M. Genetic determinants of antibiotic resistance evolution. 2018.
doi:10.15479/AT:ISTA:th1072
apa: Lukacisinova, M. (2018). Genetic determinants of antibiotic resistance evolution.
Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:th1072
chicago: Lukacisinova, Marta. “Genetic Determinants of Antibiotic Resistance Evolution.”
Institute of Science and Technology Austria, 2018. https://doi.org/10.15479/AT:ISTA:th1072.
ieee: M. Lukacisinova, “Genetic determinants of antibiotic resistance evolution,”
Institute of Science and Technology Austria, 2018.
ista: Lukacisinova M. 2018. Genetic determinants of antibiotic resistance evolution.
Institute of Science and Technology Austria.
mla: Lukacisinova, Marta. Genetic Determinants of Antibiotic Resistance Evolution.
Institute of Science and Technology Austria, 2018, doi:10.15479/AT:ISTA:th1072.
short: M. Lukacisinova, Genetic Determinants of Antibiotic Resistance Evolution,
Institute of Science and Technology Austria, 2018.
date_created: 2019-04-09T13:57:15Z
date_published: 2018-12-28T00:00:00Z
date_updated: 2023-09-22T09:20:37Z
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supervisor:
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orcid: 0000-0003-4398-476X
title: Genetic determinants of antibiotic resistance evolution
type: dissertation
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...