---
_id: '7500'
abstract:
- lang: eng
text: "Plant survival depends on vascular tissues, which originate in a self‐organizing
manner as strands of cells co‐directionally transporting the plant hormone auxin.
The latter phenomenon (also known as auxin canalization) is classically hypothesized
to be regulated by auxin itself via the effect of this hormone on the polarity
of its own intercellular transport. Correlative observations supported this concept,
but molecular insights remain limited.\r\nIn the current study, we established
an experimental system based on the model Arabidopsis thaliana, which exhibits
auxin transport channels and formation of vasculature strands in response to local
auxin application.\r\nOur methodology permits the genetic analysis of auxin canalization
under controllable experimental conditions. By utilizing this opportunity, we
confirmed the dependence of auxin canalization on a PIN‐dependent auxin transport
and nuclear, TIR1/AFB‐mediated auxin signaling. We also show that leaf venation
and auxin‐mediated PIN repolarization in the root require TIR1/AFB signaling.\r\nFurther
studies based on this experimental system are likely to yield better understanding
of the mechanisms underlying auxin transport polarization in other developmental
contexts."
acknowledgement: We thank Mark Estelle, José M. Alonso and the Arabidopsis Stock Centre
for providing seeds. We acknowledge the core facility CELLIM of CEITEC supported
by the MEYS CR (LM2015062 Czech‐BioImaging) and Plant Sciences Core Facility of
CEITEC Masaryk University for help in generating essential data. This project received
funding from the European Research Council (ERC) under the European Union's Horizon
2020 research and innovation program (grant agreement no. 742985) and the Czech
Science Foundation GAČR (GA13‐40637S and GA18‐26981S) to JF. JH is the recipient
of a DOC Fellowship of the Austrian Academy of Sciences at the Institute of Science
and Technology. The authors declare no competing interests.
article_processing_charge: No
article_type: original
author:
- first_name: E
full_name: Mazur, E
last_name: Mazur
- first_name: Ivan
full_name: Kulik, Ivan
id: F0AB3FCE-02D1-11E9-BD0E-99399A5D3DEB
last_name: Kulik
- first_name: Jakub
full_name: Hajny, Jakub
id: 4800CC20-F248-11E8-B48F-1D18A9856A87
last_name: Hajny
orcid: 0000-0003-2140-7195
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Mazur E, Kulik I, Hajny J, Friml J. Auxin canalization and vascular tissue
formation by TIR1/AFB-mediated auxin signaling in arabidopsis. New Phytologist.
2020;226(5):1375-1383. doi:10.1111/nph.16446
apa: Mazur, E., Kulik, I., Hajny, J., & Friml, J. (2020). Auxin canalization
and vascular tissue formation by TIR1/AFB-mediated auxin signaling in arabidopsis.
New Phytologist. Wiley. https://doi.org/10.1111/nph.16446
chicago: Mazur, E, Ivan Kulik, Jakub Hajny, and Jiří Friml. “Auxin Canalization
and Vascular Tissue Formation by TIR1/AFB-Mediated Auxin Signaling in Arabidopsis.”
New Phytologist. Wiley, 2020. https://doi.org/10.1111/nph.16446.
ieee: E. Mazur, I. Kulik, J. Hajny, and J. Friml, “Auxin canalization and vascular
tissue formation by TIR1/AFB-mediated auxin signaling in arabidopsis,” New
Phytologist, vol. 226, no. 5. Wiley, pp. 1375–1383, 2020.
ista: Mazur E, Kulik I, Hajny J, Friml J. 2020. Auxin canalization and vascular
tissue formation by TIR1/AFB-mediated auxin signaling in arabidopsis. New Phytologist.
226(5), 1375–1383.
mla: Mazur, E., et al. “Auxin Canalization and Vascular Tissue Formation by TIR1/AFB-Mediated
Auxin Signaling in Arabidopsis.” New Phytologist, vol. 226, no. 5, Wiley,
2020, pp. 1375–83, doi:10.1111/nph.16446.
short: E. Mazur, I. Kulik, J. Hajny, J. Friml, New Phytologist 226 (2020) 1375–1383.
date_created: 2020-02-18T10:03:47Z
date_published: 2020-06-01T00:00:00Z
date_updated: 2024-03-27T23:30:37Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/nph.16446
ec_funded: 1
external_id:
isi:
- '000514939700001'
pmid:
- '31971254'
file:
- access_level: open_access
checksum: 17de728b0205979feb95ce663ba918c2
content_type: application/pdf
creator: dernst
date_created: 2020-11-20T09:32:10Z
date_updated: 2020-11-20T09:32:10Z
file_id: '8781'
file_name: 2020_NewPhytologist_Mazur.pdf
file_size: 2106888
relation: main_file
success: 1
file_date_updated: 2020-11-20T09:32:10Z
has_accepted_license: '1'
intvolume: ' 226'
isi: 1
issue: '5'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '06'
oa: 1
oa_version: Published Version
page: 1375-1383
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 2699E3D2-B435-11E9-9278-68D0E5697425
grant_number: '25239'
name: Cell surface receptor complexes for PIN polarity and auxin-mediated development
publication: New Phytologist
publication_identifier:
eissn:
- 1469-8137
issn:
- 0028-646x
publication_status: published
publisher: Wiley
quality_controlled: '1'
related_material:
record:
- id: '8822'
relation: dissertation_contains
status: public
status: public
title: Auxin canalization and vascular tissue formation by TIR1/AFB-mediated auxin
signaling in arabidopsis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 226
year: '2020'
...
---
_id: '146'
abstract:
- lang: eng
text: The root cap protects the stem cell niche of angiosperm roots from damage.
In Arabidopsis, lateral root cap (LRC) cells covering the meristematic zone are
regularly lost through programmed cell death, while the outermost layer of the
root cap covering the tip is repeatedly sloughed. Efficient coordination with
stem cells producing new layers is needed to maintain a constant size of the cap.
We present a signalling pair, the peptide IDA-LIKE1 (IDL1) and its receptor HAESA-LIKE2
(HSL2), mediating such communication. Live imaging over several days characterized
this process from initial fractures in LRC cell files to full separation of a
layer. Enhanced expression of IDL1 in the separating root cap layers resulted
in increased frequency of sloughing, balanced with generation of new layers in
a HSL2-dependent manner. Transcriptome analyses linked IDL1-HSL2 signalling to
the transcription factors BEARSKIN1/2 and genes associated with programmed cell
death. Mutations in either IDL1 or HSL2 slowed down cell division, maturation
and separation. Thus, IDL1-HSL2 signalling potentiates dynamic regulation of the
homeostatic balance between stem cell division and sloughing activity.
article_processing_charge: No
article_type: original
author:
- first_name: Chun Lin
full_name: Shi, Chun Lin
last_name: Shi
- first_name: Daniel
full_name: Von Wangenheim, Daniel
id: 49E91952-F248-11E8-B48F-1D18A9856A87
last_name: Von Wangenheim
orcid: 0000-0002-6862-1247
- first_name: Ullrich
full_name: Herrmann, Ullrich
last_name: Herrmann
- first_name: Mari
full_name: Wildhagen, Mari
last_name: Wildhagen
- first_name: Ivan
full_name: Kulik, Ivan
id: F0AB3FCE-02D1-11E9-BD0E-99399A5D3DEB
last_name: Kulik
- first_name: Andreas
full_name: Kopf, Andreas
last_name: Kopf
- first_name: Takashi
full_name: Ishida, Takashi
last_name: Ishida
- first_name: Vilde
full_name: Olsson, Vilde
last_name: Olsson
- first_name: Mari Kristine
full_name: Anker, Mari Kristine
last_name: Anker
- first_name: Markus
full_name: Albert, Markus
last_name: Albert
- first_name: Melinka A
full_name: Butenko, Melinka A
last_name: Butenko
- first_name: Georg
full_name: Felix, Georg
last_name: Felix
- first_name: Shinichiro
full_name: Sawa, Shinichiro
last_name: Sawa
- first_name: Manfred
full_name: Claassen, Manfred
last_name: Claassen
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Reidunn B
full_name: Aalen, Reidunn B
last_name: Aalen
citation:
ama: Shi CL, von Wangenheim D, Herrmann U, et al. The dynamics of root cap sloughing
in Arabidopsis is regulated by peptide signalling. Nature Plants. 2018;4(8):596-604.
doi:10.1038/s41477-018-0212-z
apa: Shi, C. L., von Wangenheim, D., Herrmann, U., Wildhagen, M., Kulik, I., Kopf,
A., … Aalen, R. B. (2018). The dynamics of root cap sloughing in Arabidopsis is
regulated by peptide signalling. Nature Plants. Nature Publishing Group.
https://doi.org/10.1038/s41477-018-0212-z
chicago: Shi, Chun Lin, Daniel von Wangenheim, Ullrich Herrmann, Mari Wildhagen,
Ivan Kulik, Andreas Kopf, Takashi Ishida, et al. “The Dynamics of Root Cap Sloughing
in Arabidopsis Is Regulated by Peptide Signalling.” Nature Plants. Nature
Publishing Group, 2018. https://doi.org/10.1038/s41477-018-0212-z.
ieee: C. L. Shi et al., “The dynamics of root cap sloughing in Arabidopsis
is regulated by peptide signalling,” Nature Plants, vol. 4, no. 8. Nature
Publishing Group, pp. 596–604, 2018.
ista: Shi CL, von Wangenheim D, Herrmann U, Wildhagen M, Kulik I, Kopf A, Ishida
T, Olsson V, Anker MK, Albert M, Butenko MA, Felix G, Sawa S, Claassen M, Friml
J, Aalen RB. 2018. The dynamics of root cap sloughing in Arabidopsis is regulated
by peptide signalling. Nature Plants. 4(8), 596–604.
mla: Shi, Chun Lin, et al. “The Dynamics of Root Cap Sloughing in Arabidopsis Is
Regulated by Peptide Signalling.” Nature Plants, vol. 4, no. 8, Nature
Publishing Group, 2018, pp. 596–604, doi:10.1038/s41477-018-0212-z.
short: C.L. Shi, D. von Wangenheim, U. Herrmann, M. Wildhagen, I. Kulik, A. Kopf,
T. Ishida, V. Olsson, M.K. Anker, M. Albert, M.A. Butenko, G. Felix, S. Sawa,
M. Claassen, J. Friml, R.B. Aalen, Nature Plants 4 (2018) 596–604.
date_created: 2018-12-11T11:44:52Z
date_published: 2018-07-30T00:00:00Z
date_updated: 2023-09-19T10:08:45Z
day: '30'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41477-018-0212-z
external_id:
isi:
- '000443861300016'
pmid:
- '30061750'
file:
- access_level: open_access
checksum: da33101c76ee1b2dc5ab28fd2ccba9d0
content_type: application/pdf
creator: dernst
date_created: 2019-11-18T16:24:07Z
date_updated: 2020-07-14T12:44:56Z
file_id: '7043'
file_name: 2018_NaturePlants_Shi.pdf
file_size: 226829
relation: main_file
file_date_updated: 2020-07-14T12:44:56Z
has_accepted_license: '1'
intvolume: ' 4'
isi: 1
issue: '8'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Submitted Version
page: 596 - 604
pmid: 1
publication: Nature Plants
publication_status: published
publisher: Nature Publishing Group
publist_id: '7777'
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/new-process-in-root-development-discovered/
scopus_import: '1'
status: public
title: The dynamics of root cap sloughing in Arabidopsis is regulated by peptide signalling
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 4
year: '2018'
...