[{"author":[{"id":"68E56E44-62B0-11EA-B963-444F3DDC885E","first_name":"Roderich","last_name":"Römhild","full_name":"Römhild, Roderich","orcid":"0000-0001-9480-5261"},{"orcid":"0000-0003-4398-476X","full_name":"Bollenbach, Mark Tobias","last_name":"Bollenbach","id":"3E6DB97A-F248-11E8-B48F-1D18A9856A87","first_name":"Mark Tobias"},{"full_name":"Andersson, Dan I.","last_name":"Andersson","first_name":"Dan I."}],"keyword":["General Immunology and Microbiology","Microbiology","Infectious Diseases"],"publication_identifier":{"issn":["1740-1526"],"eissn":["1740-1534"]},"language":[{"iso":"eng"}],"intvolume":" 20","doi":"10.1038/s41579-022-00700-5","acknowledgement":"The authors thank B. Kavčič and H. Schulenburg for constructive feedback on the manuscript.","publication":"Nature Reviews Microbiology","publication_status":"published","status":"public","type":"journal_article","volume":20,"citation":{"ieee":"R. Römhild, M. T. Bollenbach, and D. I. Andersson, “The physiology and genetics of bacterial responses to antibiotic combinations,” Nature Reviews Microbiology, vol. 20. Springer Nature, pp. 478–490, 2022.","short":"R. Römhild, M.T. Bollenbach, D.I. Andersson, Nature Reviews Microbiology 20 (2022) 478–490.","apa":"Römhild, R., Bollenbach, M. T., & Andersson, D. I. (2022). The physiology and genetics of bacterial responses to antibiotic combinations. Nature Reviews Microbiology. Springer Nature. https://doi.org/10.1038/s41579-022-00700-5","ista":"Römhild R, Bollenbach MT, Andersson DI. 2022. The physiology and genetics of bacterial responses to antibiotic combinations. Nature Reviews Microbiology. 20, 478–490.","ama":"Römhild R, Bollenbach MT, Andersson DI. The physiology and genetics of bacterial responses to antibiotic combinations. Nature Reviews Microbiology. 2022;20:478-490. doi:10.1038/s41579-022-00700-5","mla":"Römhild, Roderich, et al. “The Physiology and Genetics of Bacterial Responses to Antibiotic Combinations.” Nature Reviews Microbiology, vol. 20, Springer Nature, 2022, pp. 478–90, doi:10.1038/s41579-022-00700-5.","chicago":"Römhild, Roderich, Mark Tobias Bollenbach, and Dan I. Andersson. “The Physiology and Genetics of Bacterial Responses to Antibiotic Combinations.” Nature Reviews Microbiology. Springer Nature, 2022. https://doi.org/10.1038/s41579-022-00700-5."},"month":"08","date_published":"2022-08-01T00:00:00Z","_id":"10812","isi":1,"department":[{"_id":"CaGu"}],"day":"01","date_updated":"2023-08-02T14:41:44Z","year":"2022","date_created":"2022-03-04T04:33:49Z","article_type":"review","quality_controlled":"1","oa_version":"None","publisher":"Springer Nature","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","title":"The physiology and genetics of bacterial responses to antibiotic combinations","article_processing_charge":"No","abstract":[{"lang":"eng","text":"Several promising strategies based on combining or cycling different antibiotics have been proposed to increase efficacy and counteract resistance evolution, but we still lack a deep understanding of the physiological responses and genetic mechanisms that underlie antibiotic interactions and the clinical applicability of these strategies. In antibiotic-exposed bacteria, the combined effects of physiological stress responses and emerging resistance mutations (occurring at different time scales) generate complex and often unpredictable dynamics. In this Review, we present our current understanding of bacterial cell physiology and genetics of responses to antibiotics. We emphasize recently discovered mechanisms of synergistic and antagonistic drug interactions, hysteresis in temporal interactions between antibiotics that arise from microbial physiology and interactions between antibiotics and resistance mutations that can cause collateral sensitivity or cross-resistance. We discuss possible connections between the different phenomena and indicate relevant research directions. A better and more unified understanding of drug and genetic interactions is likely to advance antibiotic therapy."}],"page":"478-490","scopus_import":"1","external_id":{"isi":["000763891900001"],"pmid":["35241807"]},"pmid":1},{"publication_status":"published","type":"journal_article","volume":17,"status":"public","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"citation":{"mla":"Römhild, Roderich, and Dan I. Andersson. “Mechanisms and Therapeutic Potential of Collateral Sensitivity to Antibiotics.” PLoS Pathogens, vol. 17, no. 1, e1009172, Public Library of Science, 2021, doi:10.1371/journal.ppat.1009172.","chicago":"Römhild, Roderich, and Dan I. Andersson. “Mechanisms and Therapeutic Potential of Collateral Sensitivity to Antibiotics.” PLoS Pathogens. Public Library of Science, 2021. https://doi.org/10.1371/journal.ppat.1009172.","ista":"Römhild R, Andersson DI. 2021. Mechanisms and therapeutic potential of collateral sensitivity to antibiotics. PLoS Pathogens. 17(1), e1009172.","ama":"Römhild R, Andersson DI. Mechanisms and therapeutic potential of collateral sensitivity to antibiotics. PLoS Pathogens. 2021;17(1). doi:10.1371/journal.ppat.1009172","short":"R. Römhild, D.I. Andersson, PLoS Pathogens 17 (2021).","apa":"Römhild, R., & Andersson, D. I. (2021). Mechanisms and therapeutic potential of collateral sensitivity to antibiotics. PLoS Pathogens. Public Library of Science. https://doi.org/10.1371/journal.ppat.1009172","ieee":"R. Römhild and D. I. Andersson, “Mechanisms and therapeutic potential of collateral sensitivity to antibiotics,” PLoS Pathogens, vol. 17, no. 1. Public Library of Science, 2021."},"file_date_updated":"2021-02-03T12:13:03Z","author":[{"full_name":"Römhild, Roderich","orcid":"0000-0001-9480-5261","id":"68E56E44-62B0-11EA-B963-444F3DDC885E","first_name":"Roderich","last_name":"Römhild"},{"last_name":"Andersson","first_name":"Dan I.","full_name":"Andersson, Dan I."}],"intvolume":" 17","language":[{"iso":"eng"}],"publication_identifier":{"issn":["15537366"],"eissn":["15537374"]},"publication":"PLoS Pathogens","doi":"10.1371/journal.ppat.1009172","acknowledgement":"Our work was supported by the Swedish Research Council (grant 2017-01527) to DIA","day":"14","date_updated":"2023-08-07T13:36:55Z","year":"2021","article_number":"e1009172","date_published":"2021-01-14T00:00:00Z","month":"01","department":[{"_id":"CaGu"}],"isi":1,"_id":"9046","oa_version":"Published Version","quality_controlled":"1","title":"Mechanisms and therapeutic potential of collateral sensitivity to antibiotics","publisher":"Public Library of Science","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","issue":"1","date_created":"2021-01-31T23:01:21Z","article_type":"original","ddc":["570"],"oa":1,"scopus_import":"1","pmid":1,"external_id":{"isi":["000610190400007"],"pmid":["33444399"]},"article_processing_charge":"No","file":[{"file_size":570066,"access_level":"open_access","relation":"main_file","date_updated":"2021-02-03T12:13:03Z","checksum":"d745d7f8fcbb9b95fea16a36f94dee31","creator":"dernst","file_name":"2021_PlosPathogens_Roemhild.pdf","date_created":"2021-02-03T12:13:03Z","file_id":"9070","content_type":"application/pdf","success":1}],"has_accepted_license":"1"},{"article_processing_charge":"No","abstract":[{"text":"Evolutionary adaptation is a major source of antibiotic resistance in bacterial pathogens. Evolution-informed therapy aims to constrain resistance by accounting for bacterial evolvability. Sequential treatments with antibiotics that target different bacterial processes were previously shown to limit adaptation through genetic resistance trade-offs and negative hysteresis. Treatment with homogeneous sets of antibiotics is generally viewed to be disadvantageous, as it should rapidly lead to cross-resistance. We here challenged this assumption by determining the evolutionary response of Pseudomonas aeruginosa to experimental sequential treatments involving both heterogenous and homogeneous antibiotic sets. To our surprise, we found that fast switching between only β-lactam antibiotics resulted in increased extinction of bacterial populations. We demonstrate that extinction is favored by low rates of spontaneous resistance emergence and low levels of spontaneous cross-resistance among the antibiotics in sequence. The uncovered principles may help to guide the optimized use of available antibiotics in highly potent, evolution-informed treatment designs.","lang":"eng"}],"pmid":1,"external_id":{"isi":["000692027800001"],"pmid":["34318749"]},"scopus_import":"1","article_type":"original","oa":1,"date_created":"2021-07-28T13:36:57Z","main_file_link":[{"url":"https://doi.org/10.7554/eLife.68876","open_access":"1"}],"quality_controlled":"1","oa_version":"Published Version","title":"High potency of sequential therapy with only beta-lactam antibiotics","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publisher":"eLife Sciences Publications","isi":1,"department":[{"_id":"CaGu"}],"_id":"9746","article_number":"e68876","date_published":"2021-07-28T00:00:00Z","month":"07","date_updated":"2023-08-11T10:26:29Z","year":"2021","day":"28","intvolume":" 10","language":[{"iso":"eng"}],"publication_identifier":{"eissn":["2050-084X"]},"publication":"eLife","doi":"10.7554/elife.68876","acknowledgement":"We would like to thank Leif Tueffers and João Botelho for discussions and suggestions as well as Kira Haas and Julia Bunk for technical support. We acknowledge financial support from the German Science Foundation (grant SCHU 1415/12-2 to HS, and funding under Germany’s Excellence Strategy EXC 2167–390884018 as well as the Research Training Group 2501 TransEvo to HS and SN), the Max Planck Society (IMPRS scholarship to AB; Max-Planck fellowship to HS), and the Leibniz Science Campus Evolutionary Medicine of the Lung (EvoLUNG, to HS and SN). This work was further supported by the German Science Foundation Research Infrastructure NGS_CC (project 407495230) as part of the Next Generation Sequencing Competence Network (project 423957469). NGS analyses were carried out at the Competence Centre for Genomic Analysis Kiel (CCGA Kiel).","author":[{"full_name":"Batra, Aditi","first_name":"Aditi","last_name":"Batra"},{"orcid":"0000-0001-9480-5261","full_name":"Römhild, Roderich","last_name":"Römhild","id":"68E56E44-62B0-11EA-B963-444F3DDC885E","first_name":"Roderich"},{"first_name":"Emilie","last_name":"Rousseau","full_name":"Rousseau, Emilie"},{"last_name":"Franzenburg","first_name":"Sören","full_name":"Franzenburg, Sören"},{"full_name":"Niemann, Stefan","last_name":"Niemann","first_name":"Stefan"},{"full_name":"Schulenburg, Hinrich","first_name":"Hinrich","last_name":"Schulenburg"}],"citation":{"ista":"Batra A, Römhild R, Rousseau E, Franzenburg S, Niemann S, Schulenburg H. 2021. High potency of sequential therapy with only beta-lactam antibiotics. eLife. 10, e68876.","ama":"Batra A, Römhild R, Rousseau E, Franzenburg S, Niemann S, Schulenburg H. High potency of sequential therapy with only beta-lactam antibiotics. eLife. 2021;10. doi:10.7554/elife.68876","mla":"Batra, Aditi, et al. “High Potency of Sequential Therapy with Only Beta-Lactam Antibiotics.” ELife, vol. 10, e68876, eLife Sciences Publications, 2021, doi:10.7554/elife.68876.","chicago":"Batra, Aditi, Roderich Römhild, Emilie Rousseau, Sören Franzenburg, Stefan Niemann, and Hinrich Schulenburg. “High Potency of Sequential Therapy with Only Beta-Lactam Antibiotics.” ELife. eLife Sciences Publications, 2021. https://doi.org/10.7554/elife.68876.","ieee":"A. Batra, R. Römhild, E. Rousseau, S. Franzenburg, S. Niemann, and H. Schulenburg, “High potency of sequential therapy with only beta-lactam antibiotics,” eLife, vol. 10. eLife Sciences Publications, 2021.","short":"A. Batra, R. Römhild, E. Rousseau, S. Franzenburg, S. Niemann, H. Schulenburg, ELife 10 (2021).","apa":"Batra, A., Römhild, R., Rousseau, E., Franzenburg, S., Niemann, S., & Schulenburg, H. (2021). High potency of sequential therapy with only beta-lactam antibiotics. ELife. eLife Sciences Publications. https://doi.org/10.7554/elife.68876"},"publication_status":"published","volume":10,"type":"journal_article","status":"public"}]