[{"article_type":"original","publication":"iScience","citation":{"ista":"Bartalska K, Hübschmann V, Korkut M, Cubero RJ, Venturino A, Rössler K, Czech T, Siegert S. 2022. A systematic characterization of microglia-like cell occurrence during retinal organoid differentiation. iScience. 25(7), 104580.","ieee":"K. Bartalska et al., “A systematic characterization of microglia-like cell occurrence during retinal organoid differentiation,” iScience, vol. 25, no. 7. Elsevier, 2022.","apa":"Bartalska, K., Hübschmann, V., Korkut, M., Cubero, R. J., Venturino, A., Rössler, K., … Siegert, S. (2022). A systematic characterization of microglia-like cell occurrence during retinal organoid differentiation. IScience. Elsevier. https://doi.org/10.1016/j.isci.2022.104580","ama":"Bartalska K, Hübschmann V, Korkut M, et al. A systematic characterization of microglia-like cell occurrence during retinal organoid differentiation. iScience. 2022;25(7). doi:10.1016/j.isci.2022.104580","chicago":"Bartalska, Katarina, Verena Hübschmann, Medina Korkut, Ryan J Cubero, Alessandro Venturino, Karl Rössler, Thomas Czech, and Sandra Siegert. “A Systematic Characterization of Microglia-like Cell Occurrence during Retinal Organoid Differentiation.” IScience. Elsevier, 2022. https://doi.org/10.1016/j.isci.2022.104580.","mla":"Bartalska, Katarina, et al. “A Systematic Characterization of Microglia-like Cell Occurrence during Retinal Organoid Differentiation.” IScience, vol. 25, no. 7, 104580, Elsevier, 2022, doi:10.1016/j.isci.2022.104580.","short":"K. Bartalska, V. Hübschmann, M. Korkut, R.J. Cubero, A. Venturino, K. Rössler, T. Czech, S. Siegert, IScience 25 (2022)."},"date_published":"2022-07-15T00:00:00Z","scopus_import":"1","day":"15","article_processing_charge":"Yes","has_accepted_license":"1","status":"public","ddc":["610"],"title":"A systematic characterization of microglia-like cell occurrence during retinal organoid differentiation","intvolume":" 25","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","_id":"11478","oa_version":"Published Version","file":[{"creator":"cchlebak","file_size":19400048,"content_type":"application/pdf","file_name":"2022_iScience_Bartalska.pdf","access_level":"open_access","date_created":"2022-07-04T08:19:25Z","date_updated":"2022-07-04T08:19:25Z","success":1,"checksum":"a470b74e1b3796c710189c81a4cd4329","file_id":"11480","relation":"main_file"}],"type":"journal_article","abstract":[{"lang":"eng","text":"Cerebral organoids differentiated from human-induced pluripotent stem cells (hiPSC) provide a unique opportunity to investigate brain development. However, organoids usually lack microglia, brain-resident immune cells, which are present in the early embryonic brain and participate in neuronal circuit development. Here, we find IBA1+ microglia-like cells alongside retinal cups between week 3 and 4 in 2.5D culture with an unguided retinal organoid differentiation protocol. Microglia do not infiltrate the neuroectoderm and instead enrich within non-pigmented, 3D-cystic compartments that develop in parallel to the 3D-retinal organoids. When we guide the retinal organoid differentiation with low-dosed BMP4, we prevent cup development and enhance microglia and 3D-cysts formation. Mass spectrometry identifies these 3D-cysts to express mesenchymal and epithelial markers. We confirmed this microglia-preferred environment also within the unguided protocol, providing insight into microglial behavior and migration and offer a model to study how they enter and distribute within the human brain."}],"issue":"7","quality_controlled":"1","isi":1,"project":[{"name":"Microglia action towards neuronal circuit formation and function in health and disease","call_identifier":"H2020","grant_number":"715571","_id":"25D4A630-B435-11E9-9278-68D0E5697425"},{"name":"IST Austria Open Access Fund","_id":"B67AFEDC-15C9-11EA-A837-991A96BB2854"},{"name":"How human microglia shape developing neurons during health and inflammation","grant_number":"SC19-017","_id":"9B99D380-BA93-11EA-9121-9846C619BF3A"}],"external_id":{"isi":["000830428500005"]},"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"language":[{"iso":"eng"}],"doi":"10.1016/j.isci.2022.104580","month":"07","publication_identifier":{"eissn":["2589-0042"]},"publication_status":"published","publisher":"Elsevier","department":[{"_id":"SaSi"}],"year":"2022","acknowledgement":"We thank the scientific service units at ISTA, specifically the lab support facility and imaging & optics facility for their support; Nicolas Armel for performing the Mass Spectrometry. We thank Alexandra Lang and Tanja Peilnsteiner for their help in human brain tissue collection, Rouven Schulz for his insights into the functional assays We thank all members of the Siegert group for constant feedback on the project and Margaret Maes, Rouven Schulz, and Marco Benevento for feedback on the manuscript. This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant No. 715571 to S.S.) and from the Gesellschaft für Forschungsförderung Niederösterreich (grant No. Sc19-017 to V.H.).","date_created":"2022-07-03T22:01:33Z","date_updated":"2023-11-02T12:21:33Z","volume":25,"author":[{"full_name":"Bartalska, Katarina","id":"4D883232-F248-11E8-B48F-1D18A9856A87","first_name":"Katarina","last_name":"Bartalska"},{"id":"32B7C918-F248-11E8-B48F-1D18A9856A87","first_name":"Verena","last_name":"Hübschmann","full_name":"Hübschmann, Verena"},{"full_name":"Korkut, Medina","first_name":"Medina","last_name":"Korkut","id":"4B51CE74-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4309-2251"},{"full_name":"Cubero, Ryan J","id":"850B2E12-9CD4-11E9-837F-E719E6697425","orcid":"0000-0003-0002-1867","first_name":"Ryan J","last_name":"Cubero"},{"id":"41CB84B2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2356-9403","first_name":"Alessandro","last_name":"Venturino","full_name":"Venturino, Alessandro"},{"full_name":"Rössler, Karl","first_name":"Karl","last_name":"Rössler"},{"last_name":"Czech","first_name":"Thomas","full_name":"Czech, Thomas"},{"orcid":"0000-0001-8635-0877","id":"36ACD32E-F248-11E8-B48F-1D18A9856A87","last_name":"Siegert","first_name":"Sandra","full_name":"Siegert, Sandra"}],"related_material":{"record":[{"relation":"other","status":"public","id":"12117"}]},"article_number":"104580","file_date_updated":"2022-07-04T08:19:25Z","ec_funded":1},{"article_number":"1931","file_date_updated":"2020-07-14T12:47:29Z","publication_status":"published","department":[{"_id":"SaSi"}],"publisher":"Springer Nature","year":"2019","date_created":"2019-05-13T07:58:35Z","date_updated":"2023-08-25T10:31:56Z","volume":10,"author":[{"first_name":"Hagar F.","last_name":"Moussa","full_name":"Moussa, Hagar F."},{"first_name":"Daniel","last_name":"Bsteh","full_name":"Bsteh, Daniel"},{"first_name":"Ramesh","last_name":"Yelagandula","full_name":"Yelagandula, Ramesh"},{"last_name":"Pribitzer","first_name":"Carina","full_name":"Pribitzer, Carina"},{"last_name":"Stecher","first_name":"Karin","full_name":"Stecher, Karin"},{"first_name":"Katarina","last_name":"Bartalska","id":"4D883232-F248-11E8-B48F-1D18A9856A87","full_name":"Bartalska, Katarina"},{"last_name":"Michetti","first_name":"Luca","full_name":"Michetti, Luca"},{"full_name":"Wang, Jingkui","last_name":"Wang","first_name":"Jingkui"},{"first_name":"Jorge A.","last_name":"Zepeda-Martinez","full_name":"Zepeda-Martinez, Jorge A."},{"full_name":"Elling, Ulrich","last_name":"Elling","first_name":"Ulrich"},{"full_name":"Stuckey, Jacob I.","first_name":"Jacob I.","last_name":"Stuckey"},{"full_name":"James, Lindsey I.","last_name":"James","first_name":"Lindsey I."},{"full_name":"Frye, Stephen V.","first_name":"Stephen V.","last_name":"Frye"},{"full_name":"Bell, Oliver","last_name":"Bell","first_name":"Oliver"}],"month":"04","publication_identifier":{"eissn":["20411723"]},"isi":1,"quality_controlled":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"external_id":{"isi":["000466118700002"]},"oa":1,"language":[{"iso":"eng"}],"doi":"10.1038/s41467-019-09628-6","type":"journal_article","abstract":[{"text":"Polycomb group (PcG) proteins play critical roles in the epigenetic inheritance of cell fate. The Polycomb Repressive Complexes PRC1 and PRC2 catalyse distinct chromatin modifications to enforce gene silencing, but how transcriptional repression is propagated through mitotic cell divisions remains a key unresolved question. Using reversible tethering of PcG proteins to ectopic sites in mouse embryonic stem cells, here we show that PRC1 can trigger transcriptional repression and Polycomb-dependent chromatin modifications. We find that canonical PRC1 (cPRC1), but not variant PRC1, maintains gene silencing through cell division upon reversal of tethering. Propagation of gene repression is sustained by cis-acting histone modifications, PRC2-mediated H3K27me3 and cPRC1-mediated H2AK119ub1, promoting a sequence-independent feedback mechanism for PcG protein recruitment. Thus, the distinct PRC1 complexes present in vertebrates can differentially regulate epigenetic maintenance of gene silencing, potentially enabling dynamic heritable responses to complex stimuli. Our findings reveal how PcG repression is potentially inherited in vertebrates.","lang":"eng"}],"issue":"1","ddc":["570"],"status":"public","title":"Canonical PRC1 controls sequence-independent propagation of Polycomb-mediated gene silencing","intvolume":" 10","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"6412","oa_version":"Published Version","file":[{"access_level":"open_access","file_name":"2019_NatureComm_Moussa.pdf","creator":"dernst","file_size":1223647,"content_type":"application/pdf","file_id":"6448","relation":"main_file","checksum":"6550a328335396c856db4cbdda7d2994","date_created":"2019-05-14T08:45:51Z","date_updated":"2020-07-14T12:47:29Z"}],"scopus_import":"1","day":"29","article_processing_charge":"No","has_accepted_license":"1","publication":"Nature Communications","citation":{"ama":"Moussa HF, Bsteh D, Yelagandula R, et al. Canonical PRC1 controls sequence-independent propagation of Polycomb-mediated gene silencing. Nature Communications. 2019;10(1). doi:10.1038/s41467-019-09628-6","ista":"Moussa HF, Bsteh D, Yelagandula R, Pribitzer C, Stecher K, Bartalska K, Michetti L, Wang J, Zepeda-Martinez JA, Elling U, Stuckey JI, James LI, Frye SV, Bell O. 2019. Canonical PRC1 controls sequence-independent propagation of Polycomb-mediated gene silencing. Nature Communications. 10(1), 1931.","apa":"Moussa, H. F., Bsteh, D., Yelagandula, R., Pribitzer, C., Stecher, K., Bartalska, K., … Bell, O. (2019). Canonical PRC1 controls sequence-independent propagation of Polycomb-mediated gene silencing. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-019-09628-6","ieee":"H. F. Moussa et al., “Canonical PRC1 controls sequence-independent propagation of Polycomb-mediated gene silencing,” Nature Communications, vol. 10, no. 1. Springer Nature, 2019.","mla":"Moussa, Hagar F., et al. “Canonical PRC1 Controls Sequence-Independent Propagation of Polycomb-Mediated Gene Silencing.” Nature Communications, vol. 10, no. 1, 1931, Springer Nature, 2019, doi:10.1038/s41467-019-09628-6.","short":"H.F. Moussa, D. Bsteh, R. Yelagandula, C. Pribitzer, K. Stecher, K. Bartalska, L. Michetti, J. Wang, J.A. Zepeda-Martinez, U. Elling, J.I. Stuckey, L.I. James, S.V. Frye, O. Bell, Nature Communications 10 (2019).","chicago":"Moussa, Hagar F., Daniel Bsteh, Ramesh Yelagandula, Carina Pribitzer, Karin Stecher, Katarina Bartalska, Luca Michetti, et al. “Canonical PRC1 Controls Sequence-Independent Propagation of Polycomb-Mediated Gene Silencing.” Nature Communications. Springer Nature, 2019. https://doi.org/10.1038/s41467-019-09628-6."},"date_published":"2019-04-29T00:00:00Z"}]