---
_id: '147'
abstract:
- lang: eng
text: The trafficking of subcellular cargos in eukaryotic cells crucially depends
on vesicle budding, a process mediated by ARF-GEFs (ADP-ribosylation factor guanine
nucleotide exchange factors). In plants, ARF-GEFs play essential roles in endocytosis,
vacuolar trafficking, recycling, secretion, and polar trafficking. Moreover, they
are important for plant development, mainly through controlling the polar subcellular
localization of PIN-FORMED (PIN) transporters of the plant hormone auxin. Here,
using a chemical genetics screen in Arabidopsis thaliana, we identified Endosidin
4 (ES4), an inhibitor of eukaryotic ARF-GEFs. ES4 acts similarly to and synergistically
with the established ARF-GEF inhibitor Brefeldin A and has broad effects on intracellular
trafficking, including endocytosis, exocytosis, and vacuolar targeting. Additionally,
Arabidopsis and yeast (Sacharomyces cerevisiae) mutants defective in ARF-GEF show
altered sensitivity to ES4. ES4 interferes with the activation-based membrane
association of the ARF1 GTPases, but not of their mutant variants that are activated
independently of ARF-GEF activity. Biochemical approaches and docking simulations
confirmed that ES4 specifically targets the SEC7 domain-containing ARF-GEFs. These
observations collectively identify ES4 as a chemical tool enabling the study of
ARF-GEF-mediated processes, including ARF-GEF-mediated plant development.
acknowledgement: We thank Gerd Jürgens, Sandra Richter, and Sheng Yang He for providing
antibodies; Maciek Adamowski, Fernando Aniento, Sebastian Bednarek, Nico Callewaert,
Matyás Fendrych, Elena Feraru, and Mugurel I. Feraru for helpful suggestions; Siamsa
Doyle for critical reading of the manuscript and helpful comments and suggestions;
and Stephanie Smith and Martine De Cock for help in editing and language corrections.
We acknowledge the core facility Cellular Imaging of CEITEC supported by the Czech-BioImaging
large RI project (LM2015062 funded by MEYS CR) for their support with obtaining
scientific data presented in this article. Plant Sciences Core Facility of CEITEC
Masaryk University is gratefully acknowledged for obtaining part of the scientific
data presented in this article. We acknowledge support from the Fondation pour la
Recherche Médicale and from the Institut National du Cancer (J.C.). The research
leading to these results was funded by the European Research Council under the European
Union's 7th Framework Program (FP7/2007-2013)/ERC grant agreement numbers 282300
and 742985 and the Czech Science Foundation GAČR (GA18-26981S; J.F.); Ministry of
Education, Youth, and Sports/MEYS of the Czech Republic under the Project CEITEC
2020 (LQ1601; T.N.); the China Science Council for a predoctoral fellowship (Q.L.);
a joint research project within the framework of cooperation between the Research
Foundation-Flanders and the Bulgarian Academy of Sciences (VS.025.13N; K.M. and
E.R.); Vetenskapsrådet and Vinnova (Verket för Innovationssystem; S.R.), Knut och
Alice Wallenbergs Stiftelse via “Shapesystem” Grant 2012.0050 (S.R.), Kempe stiftelserna
(P.G.), Tryggers CTS410 (P.G.).
article_processing_charge: No
article_type: original
author:
- first_name: Urszula
full_name: Kania, Urszula
id: 4AE5C486-F248-11E8-B48F-1D18A9856A87
last_name: Kania
- first_name: Tomasz
full_name: Nodzyński, Tomasz
last_name: Nodzyński
- first_name: Qing
full_name: Lu, Qing
last_name: Lu
- first_name: Glenn R
full_name: Hicks, Glenn R
last_name: Hicks
- first_name: Wim
full_name: Nerinckx, Wim
last_name: Nerinckx
- first_name: Kiril
full_name: Mishev, Kiril
last_name: Mishev
- first_name: Francois
full_name: Peurois, Francois
last_name: Peurois
- first_name: Jacqueline
full_name: Cherfils, Jacqueline
last_name: Cherfils
- first_name: Rycke Riet Maria
full_name: De, Rycke Riet Maria
last_name: De
- first_name: Peter
full_name: Grones, Peter
id: 399876EC-F248-11E8-B48F-1D18A9856A87
last_name: Grones
- first_name: Stéphanie
full_name: Robert, Stéphanie
last_name: Robert
- first_name: Eugenia
full_name: Russinova, Eugenia
last_name: Russinova
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Kania U, Nodzyński T, Lu Q, et al. The inhibitor Endosidin 4 targets SEC7 domain-type
ARF GTPase exchange factors and interferes with sub cellular trafficking in eukaryotes.
The Plant Cell. 2018;30(10):2553-2572. doi:10.1105/tpc.18.00127
apa: Kania, U., Nodzyński, T., Lu, Q., Hicks, G. R., Nerinckx, W., Mishev, K., …
Friml, J. (2018). The inhibitor Endosidin 4 targets SEC7 domain-type ARF GTPase
exchange factors and interferes with sub cellular trafficking in eukaryotes. The
Plant Cell. Oxford University Press. https://doi.org/10.1105/tpc.18.00127
chicago: Kania, Urszula, Tomasz Nodzyński, Qing Lu, Glenn R Hicks, Wim Nerinckx,
Kiril Mishev, Francois Peurois, et al. “The Inhibitor Endosidin 4 Targets SEC7
Domain-Type ARF GTPase Exchange Factors and Interferes with Sub Cellular Trafficking
in Eukaryotes.” The Plant Cell. Oxford University Press, 2018. https://doi.org/10.1105/tpc.18.00127.
ieee: U. Kania et al., “The inhibitor Endosidin 4 targets SEC7 domain-type
ARF GTPase exchange factors and interferes with sub cellular trafficking in eukaryotes,”
The Plant Cell, vol. 30, no. 10. Oxford University Press, pp. 2553–2572,
2018.
ista: Kania U, Nodzyński T, Lu Q, Hicks GR, Nerinckx W, Mishev K, Peurois F, Cherfils
J, De RRM, Grones P, Robert S, Russinova E, Friml J. 2018. The inhibitor Endosidin
4 targets SEC7 domain-type ARF GTPase exchange factors and interferes with sub
cellular trafficking in eukaryotes. The Plant Cell. 30(10), 2553–2572.
mla: Kania, Urszula, et al. “The Inhibitor Endosidin 4 Targets SEC7 Domain-Type
ARF GTPase Exchange Factors and Interferes with Sub Cellular Trafficking in Eukaryotes.”
The Plant Cell, vol. 30, no. 10, Oxford University Press, 2018, pp. 2553–72,
doi:10.1105/tpc.18.00127.
short: U. Kania, T. Nodzyński, Q. Lu, G.R. Hicks, W. Nerinckx, K. Mishev, F. Peurois,
J. Cherfils, R.R.M. De, P. Grones, S. Robert, E. Russinova, J. Friml, The Plant
Cell 30 (2018) 2553–2572.
date_created: 2018-12-11T11:44:52Z
date_published: 2018-11-12T00:00:00Z
date_updated: 2023-09-19T10:09:12Z
day: '12'
department:
- _id: JiFr
doi: 10.1105/tpc.18.00127
ec_funded: 1
external_id:
isi:
- '000450000500023'
pmid:
- '30018156'
intvolume: ' 30'
isi: 1
issue: '10'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1105/tpc.18.00127
month: '11'
oa: 1
oa_version: Published Version
page: 2553 - 2572
pmid: 1
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: The Plant Cell
publication_identifier:
issn:
- 1040-4651
publication_status: published
publisher: Oxford University Press
publist_id: '7776'
quality_controlled: '1'
scopus_import: '1'
status: public
title: The inhibitor Endosidin 4 targets SEC7 domain-type ARF GTPase exchange factors
and interferes with sub cellular trafficking in eukaryotes
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 30
year: '2018'
...
---
_id: '412'
abstract:
- lang: eng
text: Clathrin-mediated endocytosis (CME) is a cellular trafficking process in which
cargoes and lipids are internalized from the plasma membrane into vesicles coated
with clathrin and adaptor proteins. CME is essential for many developmental and
physiological processes in plants, but its underlying mechanism is not well characterised
compared to that in yeast and animal systems. Here, we searched for new factors
involved in CME in Arabidopsis thaliana by performing Tandem Affinity Purification
of proteins that interact with clathrin light chain, a principal component of
the clathrin coat. Among the confirmed interactors, we found two putative homologues
of the clathrin-coat uncoating factor auxilin previously described in non-plant
systems. Overexpression of AUXILIN-LIKE1 and AUXILIN-LIKE2 in A. thaliana caused
an arrest of seedling growth and development. This was concomitant with inhibited
endocytosis due to blocking of clathrin recruitment after the initial step of
adaptor protein binding to the plasma membrane. By contrast, auxilin-like(1/2)
loss-of-function lines did not present endocytosis-related developmental or cellular
phenotypes under normal growth conditions. This work contributes to the on-going
characterization of the endocytotic machinery in plants and provides a robust
tool for conditionally and specifically interfering with CME in A. thaliana.
acknowledgement: We thank James Matthew Watson, Monika Borowska, and Peggy Stolt-Bergner
at ProTech Facility of the Vienna Biocenter Core Facilities for the CRISPR/CAS9
construct; Anna Müller for assistance with molecular cloning; Sebastian Bednarek,
Liwen Jiang, and Daniël Van Damme for sharing published material; Matyáš Fendrych,
Daniël Van Damme, and Lindy Abas for valuable discussions; and Martine De Cock for
help with correcting the manuscript. This work was supported by the European Research
Council under the European Union Seventh Framework Programme (FP7/2007-2013)/ERC
Grant 282300 and by the Ministry of Education of the Czech Republic/MŠMT project
NPUI-LO1417.
article_processing_charge: No
article_type: original
author:
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Madhumitha
full_name: Narasimhan, Madhumitha
id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
last_name: Narasimhan
orcid: 0000-0002-8600-0671
- first_name: Urszula
full_name: Kania, Urszula
id: 4AE5C486-F248-11E8-B48F-1D18A9856A87
last_name: Kania
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: Geert
full_name: De Jaeger, Geert
last_name: De Jaeger
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Adamowski M, Narasimhan M, Kania U, Glanc M, De Jaeger G, Friml J. A functional
study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors in Arabidopsis.
The Plant Cell. 2018;30(3):700-716. doi:10.1105/tpc.17.00785
apa: Adamowski, M., Narasimhan, M., Kania, U., Glanc, M., De Jaeger, G., & Friml,
J. (2018). A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating
factors in Arabidopsis. The Plant Cell. American Society of Plant Biologists.
https://doi.org/10.1105/tpc.17.00785
chicago: Adamowski, Maciek, Madhumitha Narasimhan, Urszula Kania, Matous Glanc,
Geert De Jaeger, and Jiří Friml. “A Functional Study of AUXILIN LIKE1 and 2 Two
Putative Clathrin Uncoating Factors in Arabidopsis.” The Plant Cell. American
Society of Plant Biologists, 2018. https://doi.org/10.1105/tpc.17.00785.
ieee: M. Adamowski, M. Narasimhan, U. Kania, M. Glanc, G. De Jaeger, and J. Friml,
“A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors
in Arabidopsis,” The Plant Cell, vol. 30, no. 3. American Society of Plant
Biologists, pp. 700–716, 2018.
ista: Adamowski M, Narasimhan M, Kania U, Glanc M, De Jaeger G, Friml J. 2018. A
functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors
in Arabidopsis. The Plant Cell. 30(3), 700–716.
mla: Adamowski, Maciek, et al. “A Functional Study of AUXILIN LIKE1 and 2 Two Putative
Clathrin Uncoating Factors in Arabidopsis.” The Plant Cell, vol. 30, no.
3, American Society of Plant Biologists, 2018, pp. 700–16, doi:10.1105/tpc.17.00785.
short: M. Adamowski, M. Narasimhan, U. Kania, M. Glanc, G. De Jaeger, J. Friml,
The Plant Cell 30 (2018) 700–716.
date_created: 2018-12-11T11:46:20Z
date_published: 2018-04-09T00:00:00Z
date_updated: 2024-03-27T23:30:06Z
day: '09'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1105/tpc.17.00785
ec_funded: 1
external_id:
isi:
- '000429441400018'
pmid:
- '29511054'
file:
- access_level: open_access
checksum: 4e165e653b67d3f0684697f21aace5a1
content_type: application/pdf
creator: dernst
date_created: 2022-05-23T09:12:38Z
date_updated: 2022-05-23T09:12:38Z
file_id: '11406'
file_name: 2018_PlantCell_Adamowski.pdf
file_size: 4407538
relation: main_file
success: 1
file_date_updated: 2022-05-23T09:12:38Z
has_accepted_license: '1'
intvolume: ' 30'
isi: 1
issue: '3'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
page: 700 - 716
pmid: 1
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: The Plant Cell
publication_identifier:
eissn:
- 1532-298X
issn:
- 1040-4651
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '7417'
quality_controlled: '1'
related_material:
record:
- id: '6269'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors
in Arabidopsis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 30
year: '2018'
...
---
_id: '2188'
abstract:
- lang: eng
text: Although plant and animal cells use a similar core mechanism to deliver proteins
to the plasma membrane, their different lifestyle, body organization and specific
cell structures resulted in the acquisition of regulatory mechanisms that vary
in the two kingdoms. In particular, cell polarity regulators do not seem to be
conserved, because genes encoding key components are absent in plant genomes.
In plants, the broad knowledge on polarity derives from the study of auxin transporters,
the PIN-FORMED proteins, in the model plant Arabidopsis thaliana. In animals,
much information is provided from the study of polarity in epithelial cells that
exhibit basolateral and luminal apical polarities, separated by tight junctions.
In this review, we summarize the similarities and differences of the polarization
mechanisms between plants and animals and survey the main genetic approaches that
have been used to characterize new genes involved in polarity establishment in
plants, including the frequently used forward and reverse genetics screens as
well as a novel chemical genetics approach that is expected to overcome the limitation
of classical genetics methods.
acknowledgement: "This work was supported by a grant from the Research Foundation-Flanders
(Odysseus).\r\n\r\n"
article_number: '140017'
author:
- first_name: Urszula
full_name: Kania, Urszula
id: 4AE5C486-F248-11E8-B48F-1D18A9856A87
last_name: Kania
- first_name: Matyas
full_name: Fendrych, Matyas
last_name: Fendrych
- first_name: Jiřĺ
full_name: Friml, Jiřĺ
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Kania U, Fendrych M, Friml J. Polar delivery in plants; commonalities and differences
to animal epithelial cells. Open Biology. 2014;4(APRIL). doi:10.1098/rsob.140017
apa: Kania, U., Fendrych, M., & Friml, J. (2014). Polar delivery in plants;
commonalities and differences to animal epithelial cells. Open Biology.
Royal Society. https://doi.org/10.1098/rsob.140017
chicago: Kania, Urszula, Matyas Fendrych, and Jiří Friml. “Polar Delivery in Plants;
Commonalities and Differences to Animal Epithelial Cells.” Open Biology.
Royal Society, 2014. https://doi.org/10.1098/rsob.140017.
ieee: U. Kania, M. Fendrych, and J. Friml, “Polar delivery in plants; commonalities
and differences to animal epithelial cells,” Open Biology, vol. 4, no.
APRIL. Royal Society, 2014.
ista: Kania U, Fendrych M, Friml J. 2014. Polar delivery in plants; commonalities
and differences to animal epithelial cells. Open Biology. 4(APRIL), 140017.
mla: Kania, Urszula, et al. “Polar Delivery in Plants; Commonalities and Differences
to Animal Epithelial Cells.” Open Biology, vol. 4, no. APRIL, 140017, Royal
Society, 2014, doi:10.1098/rsob.140017.
short: U. Kania, M. Fendrych, J. Friml, Open Biology 4 (2014).
date_created: 2018-12-11T11:56:13Z
date_published: 2014-04-16T00:00:00Z
date_updated: 2021-01-12T06:55:52Z
day: '16'
ddc:
- '570'
department:
- _id: JiFr
doi: 10.1098/rsob.140017
file:
- access_level: open_access
checksum: 2020627feff36cf0799167c84149fa75
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:13:40Z
date_updated: 2020-07-14T12:45:31Z
file_id: '5025'
file_name: IST-2016-441-v1+1_140017.full.pdf
file_size: 682570
relation: main_file
file_date_updated: 2020-07-14T12:45:31Z
has_accepted_license: '1'
intvolume: ' 4'
issue: APRIL
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
publication: Open Biology
publication_status: published
publisher: Royal Society
publist_id: '4786'
pubrep_id: '441'
quality_controlled: '1'
scopus_import: 1
status: public
title: Polar delivery in plants; commonalities and differences to animal epithelial
cells
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 4
year: '2014'
...
---
_id: '2240'
abstract:
- lang: eng
text: Clathrin-mediated endocytosis is the major mechanism for eukaryotic plasma
membrane-based proteome turn-over. In plants, clathrin-mediated endocytosis is
essential for physiology and development, but the identification and organization
of the machinery operating this process remains largely obscure. Here, we identified
an eight-core-component protein complex, the TPLATE complex, essential for plant
growth via its role as major adaptor module for clathrin-mediated endocytosis.
This complex consists of evolutionarily unique proteins that associate closely
with core endocytic elements. The TPLATE complex is recruited as dynamic foci
at the plasma membrane preceding recruitment of adaptor protein complex 2, clathrin,
and dynamin-related proteins. Reduced function of different complex components
severely impaired internalization of assorted endocytic cargoes, demonstrating
its pivotal role in clathrin-mediated endocytosis. Taken together, the TPLATE
complex is an early endocytic module representing a unique evolutionary plant
adaptation of the canonical eukaryotic pathway for clathrin-mediated endocytosis.
author:
- first_name: Astrid
full_name: Gadeyne, Astrid
last_name: Gadeyne
- first_name: Clara
full_name: Sánchez Rodríguez, Clara
last_name: Sánchez Rodríguez
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Simone
full_name: Di Rubbo, Simone
last_name: Di Rubbo
- first_name: Henrik
full_name: Zauber, Henrik
last_name: Zauber
- first_name: Kevin
full_name: Vanneste, Kevin
last_name: Vanneste
- first_name: Jelle
full_name: Van Leene, Jelle
last_name: Van Leene
- first_name: Nancy
full_name: De Winne, Nancy
last_name: De Winne
- first_name: Dominique
full_name: Eeckhout, Dominique
last_name: Eeckhout
- first_name: Geert
full_name: Persiau, Geert
last_name: Persiau
- first_name: Eveline
full_name: Van De Slijke, Eveline
last_name: Van De Slijke
- first_name: Bernard
full_name: Cannoot, Bernard
last_name: Cannoot
- first_name: Leen
full_name: Vercruysse, Leen
last_name: Vercruysse
- first_name: Jonathan
full_name: Mayers, Jonathan
last_name: Mayers
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Urszula
full_name: Kania, Urszula
id: 4AE5C486-F248-11E8-B48F-1D18A9856A87
last_name: Kania
- first_name: Matthias
full_name: Ehrlich, Matthias
last_name: Ehrlich
- first_name: Alois
full_name: Schweighofer, Alois
last_name: Schweighofer
- first_name: Tijs
full_name: Ketelaar, Tijs
last_name: Ketelaar
- first_name: Steven
full_name: Maere, Steven
last_name: Maere
- first_name: Sebastian
full_name: Bednarek, Sebastian
last_name: Bednarek
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Kris
full_name: Gevaert, Kris
last_name: Gevaert
- first_name: Erwin
full_name: Witters, Erwin
last_name: Witters
- first_name: Eugenia
full_name: Russinova, Eugenia
last_name: Russinova
- first_name: Staffan
full_name: Persson, Staffan
last_name: Persson
- first_name: Geert
full_name: De Jaeger, Geert
last_name: De Jaeger
- first_name: Daniël
full_name: Van Damme, Daniël
last_name: Van Damme
citation:
ama: Gadeyne A, Sánchez Rodríguez C, Vanneste S, et al. The TPLATE adaptor complex
drives clathrin-mediated endocytosis in plants. Cell. 2014;156(4):691-704.
doi:10.1016/j.cell.2014.01.039
apa: Gadeyne, A., Sánchez Rodríguez, C., Vanneste, S., Di Rubbo, S., Zauber, H.,
Vanneste, K., … Van Damme, D. (2014). The TPLATE adaptor complex drives clathrin-mediated
endocytosis in plants. Cell. Cell Press. https://doi.org/10.1016/j.cell.2014.01.039
chicago: Gadeyne, Astrid, Clara Sánchez Rodríguez, Steffen Vanneste, Simone Di Rubbo,
Henrik Zauber, Kevin Vanneste, Jelle Van Leene, et al. “The TPLATE Adaptor Complex
Drives Clathrin-Mediated Endocytosis in Plants.” Cell. Cell Press, 2014.
https://doi.org/10.1016/j.cell.2014.01.039.
ieee: A. Gadeyne et al., “The TPLATE adaptor complex drives clathrin-mediated
endocytosis in plants,” Cell, vol. 156, no. 4. Cell Press, pp. 691–704,
2014.
ista: Gadeyne A, Sánchez Rodríguez C, Vanneste S, Di Rubbo S, Zauber H, Vanneste
K, Van Leene J, De Winne N, Eeckhout D, Persiau G, Van De Slijke E, Cannoot B,
Vercruysse L, Mayers J, Adamowski M, Kania U, Ehrlich M, Schweighofer A, Ketelaar
T, Maere S, Bednarek S, Friml J, Gevaert K, Witters E, Russinova E, Persson S,
De Jaeger G, Van Damme D. 2014. The TPLATE adaptor complex drives clathrin-mediated
endocytosis in plants. Cell. 156(4), 691–704.
mla: Gadeyne, Astrid, et al. “The TPLATE Adaptor Complex Drives Clathrin-Mediated
Endocytosis in Plants.” Cell, vol. 156, no. 4, Cell Press, 2014, pp. 691–704,
doi:10.1016/j.cell.2014.01.039.
short: A. Gadeyne, C. Sánchez Rodríguez, S. Vanneste, S. Di Rubbo, H. Zauber, K.
Vanneste, J. Van Leene, N. De Winne, D. Eeckhout, G. Persiau, E. Van De Slijke,
B. Cannoot, L. Vercruysse, J. Mayers, M. Adamowski, U. Kania, M. Ehrlich, A. Schweighofer,
T. Ketelaar, S. Maere, S. Bednarek, J. Friml, K. Gevaert, E. Witters, E. Russinova,
S. Persson, G. De Jaeger, D. Van Damme, Cell 156 (2014) 691–704.
date_created: 2018-12-11T11:56:31Z
date_published: 2014-02-13T00:00:00Z
date_updated: 2021-01-12T06:56:13Z
day: '13'
department:
- _id: JiFr
doi: 10.1016/j.cell.2014.01.039
intvolume: ' 156'
issue: '4'
language:
- iso: eng
month: '02'
oa_version: None
page: 691 - 704
publication: Cell
publication_identifier:
issn:
- '00928674'
publication_status: published
publisher: Cell Press
publist_id: '4721'
quality_controlled: '1'
scopus_import: 1
status: public
title: The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 156
year: '2014'
...
---
_id: '2919'
abstract:
- lang: eng
text: The distribution of the phytohormone auxin regulates many aspects of plant
development including growth response to gravity. Gravitropic root curvature involves
coordinated and asymmetric cell elongation between the lower and upper side of
the root, mediated by differential cellular auxin levels. The asymmetry in the
auxin distribution is established and maintained by a spatio-temporal regulation
of the PIN-FORMED (PIN) auxin transporter activity. We provide novel insights
into the complex regulation of PIN abundance and activity during root gravitropism.
We show that PIN2 turnover is differentially regulated on the upper and lower
side of gravistimulated roots by distinct but partially overlapping auxin feedback
mechanisms. In addition to regulating transcription and clathrin-mediated internalization,
auxin also controls PIN abundance at the plasma membrane by promoting their vacuolar
targeting and degradation. This effect of elevated auxin levels requires the activity
of SKP-Cullin-F-box TIR1/AFB (SCF TIR1/AFB)-dependent pathway. Importantly, also
suboptimal auxin levels mediate PIN degradation utilizing the same signalling
pathway. These feedback mechanisms are functionally important during gravitropic
response and ensure fine-tuning of auxin fluxes for maintaining as well as terminating
asymmetric growth.
author:
- first_name: Pawel
full_name: Baster, Pawel
id: 3028BD74-F248-11E8-B48F-1D18A9856A87
last_name: Baster
- first_name: Stéphanie
full_name: Robert, Stéphanie
last_name: Robert
- first_name: Jürgen
full_name: Kleine Vehn, Jürgen
last_name: Kleine Vehn
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Urszula
full_name: Kania, Urszula
id: 4AE5C486-F248-11E8-B48F-1D18A9856A87
last_name: Kania
- first_name: Wim
full_name: Grunewald, Wim
last_name: Grunewald
- first_name: Bert
full_name: De Rybel, Bert
last_name: De Rybel
- first_name: Tom
full_name: Beeckman, Tom
last_name: Beeckman
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Baster P, Robert S, Kleine Vehn J, et al. SCF^TIR1 AFB-auxin signalling regulates
PIN vacuolar trafficking and auxin fluxes during root gravitropism. EMBO Journal.
2013;32(2):260-274. doi:10.1038/emboj.2012.310
apa: Baster, P., Robert, S., Kleine Vehn, J., Vanneste, S., Kania, U., Grunewald,
W., … Friml, J. (2013). SCF^TIR1 AFB-auxin signalling regulates PIN vacuolar trafficking
and auxin fluxes during root gravitropism. EMBO Journal. Wiley-Blackwell.
https://doi.org/10.1038/emboj.2012.310
chicago: Baster, Pawel, Stéphanie Robert, Jürgen Kleine Vehn, Steffen Vanneste,
Urszula Kania, Wim Grunewald, Bert De Rybel, Tom Beeckman, and Jiří Friml. “SCF^TIR1
AFB-Auxin Signalling Regulates PIN Vacuolar Trafficking and Auxin Fluxes during
Root Gravitropism.” EMBO Journal. Wiley-Blackwell, 2013. https://doi.org/10.1038/emboj.2012.310.
ieee: P. Baster et al., “SCF^TIR1 AFB-auxin signalling regulates PIN vacuolar
trafficking and auxin fluxes during root gravitropism,” EMBO Journal, vol.
32, no. 2. Wiley-Blackwell, pp. 260–274, 2013.
ista: Baster P, Robert S, Kleine Vehn J, Vanneste S, Kania U, Grunewald W, De Rybel
B, Beeckman T, Friml J. 2013. SCF^TIR1 AFB-auxin signalling regulates PIN vacuolar
trafficking and auxin fluxes during root gravitropism. EMBO Journal. 32(2), 260–274.
mla: Baster, Pawel, et al. “SCF^TIR1 AFB-Auxin Signalling Regulates PIN Vacuolar
Trafficking and Auxin Fluxes during Root Gravitropism.” EMBO Journal, vol.
32, no. 2, Wiley-Blackwell, 2013, pp. 260–74, doi:10.1038/emboj.2012.310.
short: P. Baster, S. Robert, J. Kleine Vehn, S. Vanneste, U. Kania, W. Grunewald,
B. De Rybel, T. Beeckman, J. Friml, EMBO Journal 32 (2013) 260–274.
date_created: 2018-12-11T12:00:20Z
date_published: 2013-01-23T00:00:00Z
date_updated: 2021-01-12T07:00:41Z
day: '23'
department:
- _id: JiFr
doi: 10.1038/emboj.2012.310
external_id:
pmid:
- '23211744'
intvolume: ' 32'
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3553380/
month: '01'
oa: 1
oa_version: Submitted Version
page: 260 - 274
pmid: 1
publication: EMBO Journal
publication_status: published
publisher: Wiley-Blackwell
publist_id: '3818'
quality_controlled: '1'
scopus_import: 1
status: public
title: SCF^TIR1 AFB-auxin signalling regulates PIN vacuolar trafficking and auxin
fluxes during root gravitropism
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 32
year: '2013'
...
---
_id: '3110'
abstract:
- lang: eng
text: 'The directional transport of the phytohormone auxin depends on the phosphorylation
status and polar localization of PIN-FORMED (PIN) auxin efflux proteins. While
PINIOD (PID) kinase is directly involved in the phosphorylation of PIN proteins,
the phosphatase holoenzyme complexes that dephosphorylate PIN proteins remain
elusive. Here, we demonstrate that mutations simultaneously disrupting the function
of Arabidopsis thaliana FyPP1 (for Phytochrome-associated serine/threonine protein
phosphatase1) and FyPP3, two homologous genes encoding the catalytic subunits
of protein phosphatase6 (PP6), cause elevated accumulation of phosphorylated PIN
proteins, correlating with a basal-to-apical shift in subcellular PIN localization.
The changes in PIN polarity result in increased root basipetal auxin transport
and severe defects, including shorter roots, fewer lateral roots, defective columella
cells, root meristem collapse, abnormal cotyledons (small, cup-shaped, or fused
cotyledons), and altered leaf venation. Our molecular, biochemical, and genetic
data support the notion that FyPP1/3, SAL (for SAPS DOMAIN-LIKE), and PP2AA proteins
(RCN1 [for ROOTS CURL IN NAPHTHYLPHTHALAMIC ACID1] or PP2AA1, PP2AA2, and PP2AA3)
physically interact to form a novel PP6-type heterotrimeric holoenzyme complex.
We also show that FyPP1/3, SAL, and PP2AA interact with a subset of PIN proteins
and that for SAL the strength of the interaction depends on the PIN phosphorylation
status. Thus, an Arabidopsis PP6-type phosphatase holoenzyme acts antagonistically
with PID to direct auxin transport polarity and plant development by directly
regulating PIN phosphorylation. '
author:
- first_name: Mingqiu
full_name: Dai, Mingqiu
last_name: Dai
- first_name: Chen
full_name: Zhang, Chen
last_name: Zhang
- first_name: Urszula
full_name: Urszula Kania
id: 4AE5C486-F248-11E8-B48F-1D18A9856A87
last_name: Kania
- first_name: Fang
full_name: Chen, Fang
last_name: Chen
- first_name: Qin
full_name: Xue, Qin
last_name: Xue
- first_name: Tyra
full_name: McCray, Tyra
last_name: Mccray
- first_name: Gang
full_name: Li, Gang
last_name: Li
- first_name: Genji
full_name: Qin, Genji
last_name: Qin
- first_name: Michelle
full_name: Wakeley, Michelle
last_name: Wakeley
- first_name: William
full_name: Terzaghi, William
last_name: Terzaghi
- first_name: Jianmin
full_name: Wan, Jianmin
last_name: Wan
- first_name: Yunde
full_name: Zhao, Yunde
last_name: Zhao
- first_name: Jian
full_name: Xu, Jian
last_name: Xu
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Xing
full_name: Deng, Xing W
last_name: Deng
- first_name: Haiyang
full_name: Wang, Haiyang
last_name: Wang
citation:
ama: Dai M, Zhang C, Kania U, et al. A PP6 type phosphatase holoenzyme directly
regulates PIN phosphorylation and auxin efflux in Arabidopsis. Plant Cell.
2012;24(6):2497-2514. doi:10.1105/tpc.112.098905
apa: Dai, M., Zhang, C., Kania, U., Chen, F., Xue, Q., Mccray, T., … Wang, H. (2012).
A PP6 type phosphatase holoenzyme directly regulates PIN phosphorylation and auxin
efflux in Arabidopsis. Plant Cell. American Society of Plant Biologists.
https://doi.org/10.1105/tpc.112.098905
chicago: Dai, Mingqiu, Chen Zhang, Urszula Kania, Fang Chen, Qin Xue, Tyra Mccray,
Gang Li, et al. “A PP6 Type Phosphatase Holoenzyme Directly Regulates PIN Phosphorylation
and Auxin Efflux in Arabidopsis.” Plant Cell. American Society of Plant
Biologists, 2012. https://doi.org/10.1105/tpc.112.098905.
ieee: M. Dai et al., “A PP6 type phosphatase holoenzyme directly regulates
PIN phosphorylation and auxin efflux in Arabidopsis,” Plant Cell, vol.
24, no. 6. American Society of Plant Biologists, pp. 2497–2514, 2012.
ista: Dai M, Zhang C, Kania U, Chen F, Xue Q, Mccray T, Li G, Qin G, Wakeley M,
Terzaghi W, Wan J, Zhao Y, Xu J, Friml J, Deng X, Wang H. 2012. A PP6 type phosphatase
holoenzyme directly regulates PIN phosphorylation and auxin efflux in Arabidopsis.
Plant Cell. 24(6), 2497–2514.
mla: Dai, Mingqiu, et al. “A PP6 Type Phosphatase Holoenzyme Directly Regulates
PIN Phosphorylation and Auxin Efflux in Arabidopsis.” Plant Cell, vol.
24, no. 6, American Society of Plant Biologists, 2012, pp. 2497–514, doi:10.1105/tpc.112.098905.
short: M. Dai, C. Zhang, U. Kania, F. Chen, Q. Xue, T. Mccray, G. Li, G. Qin, M.
Wakeley, W. Terzaghi, J. Wan, Y. Zhao, J. Xu, J. Friml, X. Deng, H. Wang, Plant
Cell 24 (2012) 2497–2514.
date_created: 2018-12-11T12:01:27Z
date_published: 2012-06-01T00:00:00Z
date_updated: 2021-01-12T07:41:08Z
day: '01'
doi: 10.1105/tpc.112.098905
extern: 1
intvolume: ' 24'
issue: '6'
month: '06'
page: 2497 - 2514
publication: Plant Cell
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '3589'
quality_controlled: 0
status: public
title: A PP6 type phosphatase holoenzyme directly regulates PIN phosphorylation and
auxin efflux in Arabidopsis
type: journal_article
volume: 24
year: '2012'
...