[{"quality_controlled":"1","publisher":"MDPI","oa":1,"acknowledgement":"This research was supported by a postdoctoral fellowship of the Carl Tryggers Foundation (to K.Ö.) and by grants from Vetenskapsrådet (Nr.: 621-2004-2921 to L.B.) and VINNOVA (to L.B. and S.R.).\r\nWe thank Frederic Berger, Hidehiro Fukaki, Malcolm Bennett, Claudia Köhler, Jiri Friml for providing pRBR1::RBR1-RFP, ssl2-1, slr-1, pPKL::PKL-GFP seeds and the DR5 expressing vector, respectively. Authors are grateful to Hayashi Kenichiro for providing the auxinol compound and to Rishi Bhalerao for stimulating discussions. The technical help of Adeline Rigal and Thomas Vain with the auxinol experiments is much appreciated.","date_published":"2021-04-08T00:00:00Z","doi":"10.3390/ijms22083862","date_created":"2021-04-18T22:01:41Z","day":"08","publication":"International Journal of Molecular Sciences","isi":1,"has_accepted_license":"1","year":"2021","article_number":"3862","title":"Pickle recruits retinoblastoma related 1 to control lateral root formation in arabidopsis","author":[{"orcid":"0000-0002-5503-4983","full_name":"Ötvös, Krisztina","last_name":"Ötvös","id":"29B901B0-F248-11E8-B48F-1D18A9856A87","first_name":"Krisztina"},{"full_name":"Miskolczi, Pál","last_name":"Miskolczi","first_name":"Pál"},{"last_name":"Marhavý","full_name":"Marhavý, Peter","orcid":"0000-0001-5227-5741","first_name":"Peter","id":"3F45B078-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Alfredo","full_name":"Cruz-Ramírez, Alfredo","last_name":"Cruz-Ramírez"},{"orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","last_name":"Benková","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva"},{"last_name":"Robert","full_name":"Robert, Stéphanie","first_name":"Stéphanie"},{"full_name":"Bakó, László","last_name":"Bakó","first_name":"László"}],"article_processing_charge":"No","external_id":{"isi":["000644394800001"]},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"chicago":"Ötvös, Krisztina, Pál Miskolczi, Peter Marhavý, Alfredo Cruz-Ramírez, Eva Benková, Stéphanie Robert, and László Bakó. “Pickle Recruits Retinoblastoma Related 1 to Control Lateral Root Formation in Arabidopsis.” International Journal of Molecular Sciences. MDPI, 2021. https://doi.org/10.3390/ijms22083862.","ista":"Ötvös K, Miskolczi P, Marhavý P, Cruz-Ramírez A, Benková E, Robert S, Bakó L. 2021. Pickle recruits retinoblastoma related 1 to control lateral root formation in arabidopsis. International Journal of Molecular Sciences. 22(8), 3862.","mla":"Ötvös, Krisztina, et al. “Pickle Recruits Retinoblastoma Related 1 to Control Lateral Root Formation in Arabidopsis.” International Journal of Molecular Sciences, vol. 22, no. 8, 3862, MDPI, 2021, doi:10.3390/ijms22083862.","short":"K. Ötvös, P. Miskolczi, P. Marhavý, A. Cruz-Ramírez, E. Benková, S. Robert, L. Bakó, International Journal of Molecular Sciences 22 (2021).","ieee":"K. Ötvös et al., “Pickle recruits retinoblastoma related 1 to control lateral root formation in arabidopsis,” International Journal of Molecular Sciences, vol. 22, no. 8. MDPI, 2021.","apa":"Ötvös, K., Miskolczi, P., Marhavý, P., Cruz-Ramírez, A., Benková, E., Robert, S., & Bakó, L. (2021). Pickle recruits retinoblastoma related 1 to control lateral root formation in arabidopsis. International Journal of Molecular Sciences. MDPI. https://doi.org/10.3390/ijms22083862","ama":"Ötvös K, Miskolczi P, Marhavý P, et al. Pickle recruits retinoblastoma related 1 to control lateral root formation in arabidopsis. International Journal of Molecular Sciences. 2021;22(8). doi:10.3390/ijms22083862"},"month":"04","intvolume":" 22","scopus_import":"1","oa_version":"Published Version","abstract":[{"lang":"eng","text":"Lateral root (LR) formation is an example of a plant post-embryonic organogenesis event. LRs are issued from non-dividing cells entering consecutive steps of formative divisions, proliferation and elongation. The chromatin remodeling protein PICKLE (PKL) negatively regulates auxin-mediated LR formation through a mechanism that is not yet known. Here we show that PKL interacts with RETINOBLASTOMA-RELATED 1 (RBR1) to repress the LATERAL ORGAN BOUNDARIES-DOMAIN 16 (LBD16) promoter activity. Since LBD16 function is required for the formative division of LR founder cells, repression mediated by the PKL–RBR1 complex negatively regulates formative division and LR formation. Inhibition of LR formation by PKL–RBR1 is counteracted by auxin, indicating that, in addition to auxin-mediated transcriptional responses, the fine-tuned process of LR formation is also controlled at the chromatin level in an auxin-signaling dependent manner."}],"issue":"8","volume":22,"file":[{"access_level":"open_access","relation":"main_file","content_type":"application/pdf","file_id":"9342","checksum":"26ada2531ad1f9c01a1664de0431f1fe","success":1,"creator":"dernst","date_updated":"2021-04-19T10:54:55Z","file_size":2769717,"date_created":"2021-04-19T10:54:55Z","file_name":"2021_JourMolecularScience_Oetvoes.pdf"}],"language":[{"iso":"eng"}],"publication_identifier":{"issn":["1661-6596"],"eissn":["1422-0067"]},"publication_status":"published","status":"public","type":"journal_article","article_type":"original","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"_id":"9332","file_date_updated":"2021-04-19T10:54:55Z","department":[{"_id":"EvBe"}],"ddc":["570"],"date_updated":"2023-08-08T13:09:58Z"},{"acknowledgement":"This paper is dedicated to deceased P. Galuszka for his support and contribution to the project. This research was supported by the Scientific Service Units (SSU) of IST-Austria through resources provided by the Bioimaging Facility (BIF), the Life Science Facility (LSF) and by Centre of the Region Haná (CRH), Palacký University. We thank Lucia Hlusková, Zuzana Pěkná and Martin Hönig for technical assistance, and Fernando Aniento, Rashed Abualia and Andrej Hurný for sharing material. The work was supported from ERDF project “Plants as a tool for sustainable global development” (No. CZ.02.1.01/0.0/0.0/16_019/0000827), from Czech Science Foundation via projects 16-04184S (O.P., K.K. and K.D.), 18-23972Y (D.Z., K.K.), 17-21122S (K.B.), Erasmus+ (K.K.), Endowment Fund of Palacký University (K.K.) and EMBO Long-Term Fellowship, ALTF number 710-2016 (J.C.M.); People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013) under REA grant agreement no. [291734] (N.C.); DOC Fellowship of the Austrian Academy of Sciences at the Institute of Science and Technology, Austria (H.S.).","publisher":"Springer Nature","quality_controlled":"1","oa":1,"day":"27","publication":"Nature Communications","isi":1,"has_accepted_license":"1","year":"2020","doi":"10.1038/s41467-020-17949-0","date_published":"2020-08-27T00:00:00Z","date_created":"2020-09-06T22:01:12Z","article_number":"4285","project":[{"_id":"25681D80-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","grant_number":"291734","name":"International IST Postdoc Fellowship Programme"},{"name":"Molecular mechanisms of the cytokinin regulated endomembrane trafficking to coordinate plant organogenesis.","grant_number":"24746","_id":"261821BC-B435-11E9-9278-68D0E5697425"},{"_id":"253E54C8-B435-11E9-9278-68D0E5697425","name":"Molecular mechanism of auxindriven formative divisions delineating lateral root organogenesis in plants","grant_number":"ALTF710-2016"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"mla":"Kubiasova, Karolina, et al. “Cytokinin Fluoroprobe Reveals Multiple Sites of Cytokinin Perception at Plasma Membrane and Endoplasmic Reticulum.” Nature Communications, vol. 11, 4285, Springer Nature, 2020, doi:10.1038/s41467-020-17949-0.","apa":"Kubiasova, K., Montesinos López, J. C., Šamajová, O., Nisler, J., Mik, V., Semerádová, H., … Spíchal, L. (2020). Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-020-17949-0","ama":"Kubiasova K, Montesinos López JC, Šamajová O, et al. Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum. Nature Communications. 2020;11. doi:10.1038/s41467-020-17949-0","ieee":"K. Kubiasova et al., “Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum,” Nature Communications, vol. 11. Springer Nature, 2020.","short":"K. Kubiasova, J.C. Montesinos López, O. Šamajová, J. Nisler, V. Mik, H. Semerádová, L. Plíhalová, O. Novák, P. Marhavý, N. Cavallari, D. Zalabák, K. Berka, K. Doležal, P. Galuszka, J. Šamaj, M. Strnad, E. Benková, O. Plíhal, L. Spíchal, Nature Communications 11 (2020).","chicago":"Kubiasova, Karolina, Juan C Montesinos López, Olga Šamajová, Jaroslav Nisler, Václav Mik, Hana Semerádová, Lucie Plíhalová, et al. “Cytokinin Fluoroprobe Reveals Multiple Sites of Cytokinin Perception at Plasma Membrane and Endoplasmic Reticulum.” Nature Communications. Springer Nature, 2020. https://doi.org/10.1038/s41467-020-17949-0.","ista":"Kubiasova K, Montesinos López JC, Šamajová O, Nisler J, Mik V, Semerádová H, Plíhalová L, Novák O, Marhavý P, Cavallari N, Zalabák D, Berka K, Doležal K, Galuszka P, Šamaj J, Strnad M, Benková E, Plíhal O, Spíchal L. 2020. Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum. Nature Communications. 11, 4285."},"title":"Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum","author":[{"id":"946011F4-3E71-11EA-860B-C7A73DDC885E","first_name":"Karolina","last_name":"Kubiasova","full_name":"Kubiasova, Karolina","orcid":"0000-0001-5630-9419"},{"first_name":"Juan C","id":"310A8E3E-F248-11E8-B48F-1D18A9856A87","last_name":"Montesinos López","full_name":"Montesinos López, Juan C","orcid":"0000-0001-9179-6099"},{"last_name":"Šamajová","full_name":"Šamajová, Olga","first_name":"Olga"},{"last_name":"Nisler","full_name":"Nisler, Jaroslav","first_name":"Jaroslav"},{"first_name":"Václav","last_name":"Mik","full_name":"Mik, Václav"},{"full_name":"Semeradova, Hana","last_name":"Semeradova","id":"42FE702E-F248-11E8-B48F-1D18A9856A87","first_name":"Hana"},{"last_name":"Plíhalová","full_name":"Plíhalová, Lucie","first_name":"Lucie"},{"last_name":"Novák","full_name":"Novák, Ondřej","first_name":"Ondřej"},{"last_name":"Marhavý","orcid":"0000-0001-5227-5741","full_name":"Marhavý, Peter","id":"3F45B078-F248-11E8-B48F-1D18A9856A87","first_name":"Peter"},{"last_name":"Cavallari","full_name":"Cavallari, Nicola","id":"457160E6-F248-11E8-B48F-1D18A9856A87","first_name":"Nicola"},{"last_name":"Zalabák","full_name":"Zalabák, David","first_name":"David"},{"first_name":"Karel","last_name":"Berka","full_name":"Berka, Karel"},{"first_name":"Karel","last_name":"Doležal","full_name":"Doležal, Karel"},{"last_name":"Galuszka","full_name":"Galuszka, Petr","first_name":"Petr"},{"last_name":"Šamaj","full_name":"Šamaj, Jozef","first_name":"Jozef"},{"full_name":"Strnad, Miroslav","last_name":"Strnad","first_name":"Miroslav"},{"full_name":"Benková, Eva","orcid":"0000-0002-8510-9739","last_name":"Benková","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Ondřej","full_name":"Plíhal, Ondřej","last_name":"Plíhal"},{"first_name":"Lukáš","last_name":"Spíchal","full_name":"Spíchal, Lukáš"}],"external_id":{"isi":["000567931000002"],"pmid":["32855390"]},"article_processing_charge":"No","oa_version":"Published Version","pmid":1,"abstract":[{"text":"Plant hormone cytokinins are perceived by a subfamily of sensor histidine kinases (HKs), which via a two-component phosphorelay cascade activate transcriptional responses in the nucleus. Subcellular localization of the receptors proposed the endoplasmic reticulum (ER) membrane as a principal cytokinin perception site, while study of cytokinin transport pointed to the plasma membrane (PM)-mediated cytokinin signalling. Here, by detailed monitoring of subcellular localizations of the fluorescently labelled natural cytokinin probe and the receptor ARABIDOPSIS HISTIDINE KINASE 4 (CRE1/AHK4) fused to GFP reporter, we show that pools of the ER-located cytokinin receptors can enter the secretory pathway and reach the PM in cells of the root apical meristem, and the cell plate of dividing meristematic cells. Brefeldin A (BFA) experiments revealed vesicular recycling of the receptor and its accumulation in BFA compartments. We provide a revised view on cytokinin signalling and the possibility of multiple sites of perception at PM and ER.","lang":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"month":"08","intvolume":" 11","scopus_import":"1","file":[{"date_created":"2020-09-10T08:05:19Z","file_name":"2020_NatureComm_Kubiasova.pdf","date_updated":"2020-09-10T08:05:19Z","file_size":3455704,"creator":"dernst","checksum":"7494b7665b3d2bf2d8edb13e4f12b92d","file_id":"8357","success":1,"content_type":"application/pdf","access_level":"open_access","relation":"main_file"}],"language":[{"iso":"eng"}],"publication_identifier":{"eissn":["20411723"]},"publication_status":"published","volume":11,"ec_funded":1,"_id":"8336","status":"public","article_type":"original","type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"ddc":["580"],"date_updated":"2023-08-22T09:09:06Z","file_date_updated":"2020-09-10T08:05:19Z","department":[{"_id":"EvBe"}]},{"title":"A SOSEKI-based coordinate system interprets global polarity cues in arabidopsis","article_processing_charge":"No","external_id":{"isi":["000460479600014"]},"author":[{"first_name":"Saiko","id":"2E46069C-F248-11E8-B48F-1D18A9856A87","full_name":"Yoshida, Saiko","last_name":"Yoshida"},{"first_name":"Alja","last_name":"Van Der Schuren","full_name":"Van Der Schuren, Alja"},{"first_name":"Maritza","last_name":"Van Dop","full_name":"Van Dop, Maritza"},{"first_name":"Luc","last_name":"Van Galen","full_name":"Van Galen, Luc"},{"first_name":"Shunsuke","last_name":"Saiga","full_name":"Saiga, Shunsuke"},{"full_name":"Adibi, Milad","last_name":"Adibi","first_name":"Milad"},{"first_name":"Barbara","full_name":"Möller, Barbara","last_name":"Möller"},{"full_name":"Ten Hove, Colette A.","last_name":"Ten Hove","first_name":"Colette A."},{"first_name":"Peter","id":"3F45B078-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5227-5741","full_name":"Marhavy, Peter","last_name":"Marhavy"},{"first_name":"Richard","last_name":"Smith","full_name":"Smith, Richard"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","last_name":"Friml","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Dolf","last_name":"Weijers","full_name":"Weijers, Dolf"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"chicago":"Yoshida, Saiko, Alja Van Der Schuren, Maritza Van Dop, Luc Van Galen, Shunsuke Saiga, Milad Adibi, Barbara Möller, et al. “A SOSEKI-Based Coordinate System Interprets Global Polarity Cues in Arabidopsis.” Nature Plants. Springer Nature, 2019. https://doi.org/10.1038/s41477-019-0363-6.","ista":"Yoshida S, Van Der Schuren A, Van Dop M, Van Galen L, Saiga S, Adibi M, Möller B, Ten Hove CA, Marhavý P, Smith R, Friml J, Weijers D. 2019. A SOSEKI-based coordinate system interprets global polarity cues in arabidopsis. Nature Plants. 5(2), 160–166.","mla":"Yoshida, Saiko, et al. “A SOSEKI-Based Coordinate System Interprets Global Polarity Cues in Arabidopsis.” Nature Plants, vol. 5, no. 2, Springer Nature, 2019, pp. 160–66, doi:10.1038/s41477-019-0363-6.","ieee":"S. Yoshida et al., “A SOSEKI-based coordinate system interprets global polarity cues in arabidopsis,” Nature Plants, vol. 5, no. 2. Springer Nature, pp. 160–166, 2019.","short":"S. Yoshida, A. Van Der Schuren, M. Van Dop, L. Van Galen, S. Saiga, M. Adibi, B. Möller, C.A. Ten Hove, P. Marhavý, R. Smith, J. Friml, D. Weijers, Nature Plants 5 (2019) 160–166.","apa":"Yoshida, S., Van Der Schuren, A., Van Dop, M., Van Galen, L., Saiga, S., Adibi, M., … Weijers, D. (2019). A SOSEKI-based coordinate system interprets global polarity cues in arabidopsis. Nature Plants. Springer Nature. https://doi.org/10.1038/s41477-019-0363-6","ama":"Yoshida S, Van Der Schuren A, Van Dop M, et al. A SOSEKI-based coordinate system interprets global polarity cues in arabidopsis. Nature Plants. 2019;5(2):160-166. doi:10.1038/s41477-019-0363-6"},"project":[{"grant_number":"291734","name":"International IST Postdoc Fellowship Programme","_id":"25681D80-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"}],"date_created":"2019-02-17T22:59:21Z","doi":"10.1038/s41477-019-0363-6","date_published":"2019-02-08T00:00:00Z","page":"160-166","publication":"Nature Plants","day":"08","year":"2019","isi":1,"oa":1,"publisher":"Springer Nature","quality_controlled":"1","department":[{"_id":"JiFr"},{"_id":"EvBe"}],"date_updated":"2023-08-24T14:46:47Z","status":"public","type":"journal_article","_id":"6023","ec_funded":1,"issue":"2","volume":5,"language":[{"iso":"eng"}],"publication_status":"published","intvolume":" 5","month":"02","main_file_link":[{"url":"https://www.biorxiv.org/content/10.1101/479113v1.abstract","open_access":"1"}],"scopus_import":"1","oa_version":"Submitted Version","abstract":[{"text":"Multicellular development requires coordinated cell polarization relative to body axes, and translation to oriented cell division 1–3 . In plants, it is unknown how cell polarities are connected to organismal axes and translated to division. Here, we identify Arabidopsis SOSEKI proteins that integrate apical–basal and radial organismal axes to localize to polar cell edges. Localization does not depend on tissue context, requires cell wall integrity and is defined by a transferrable, protein-specific motif. A Domain of Unknown Function in SOSEKI proteins resembles the DIX oligomerization domain in the animal Dishevelled polarity regulator. The DIX-like domain self-interacts and is required for edge localization and for influencing division orientation, together with a second domain that defines the polar membrane domain. Our work shows that SOSEKI proteins locally interpret global polarity cues and can influence cell division orientation. Furthermore, this work reveals that, despite fundamental differences, cell polarity mechanisms in plants and animals converge on a similar protein domain.","lang":"eng"}]},{"status":"public","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"type":"journal_article","_id":"6351","file_date_updated":"2020-07-14T12:47:28Z","department":[{"_id":"JiFr"},{"_id":"EvBe"}],"ddc":["570"],"date_updated":"2024-03-27T23:30:10Z","intvolume":" 177","month":"05","scopus_import":"1","pmid":1,"oa_version":"Published Version","abstract":[{"text":"A process of restorative patterning in plant roots correctly replaces eliminated cells to heal local injuries despite the absence of cell migration, which underpins wound healing in animals. \r\n\r\nPatterning in plants relies on oriented cell divisions and acquisition of specific cell identities. Plants regularly endure wounds caused by abiotic or biotic environmental stimuli and have developed extraordinary abilities to restore their tissues after injuries. Here, we provide insight into a mechanism of restorative patterning that repairs tissues after wounding. Laser-assisted elimination of different cells in Arabidopsis root combined with live-imaging tracking during vertical growth allowed analysis of the regeneration processes in vivo. Specifically, the cells adjacent to the inner side of the injury re-activated their stem cell transcriptional programs. They accelerated their progression through cell cycle, coordinately changed the cell division orientation, and ultimately acquired de novo the correct cell fates to replace missing cells. These observations highlight existence of unknown intercellular positional signaling and demonstrate the capability of specified cells to re-acquire stem cell programs as a crucial part of the plant-specific mechanism of wound healing.","lang":"eng"}],"acknowledged_ssus":[{"_id":"Bio"}],"ec_funded":1,"related_material":{"record":[{"id":"9992","status":"public","relation":"dissertation_contains"}],"link":[{"url":"https://ist.ac.at/en/news/specialized-plant-cells-regain-stem-cell-features-to-heal-wounds/","relation":"press_release","description":"News on IST Homepage"}]},"volume":177,"issue":"4","language":[{"iso":"eng"}],"file":[{"file_size":10272032,"date_updated":"2020-07-14T12:47:28Z","creator":"dernst","file_name":"2019_Cell_Marhava.pdf","date_created":"2019-05-13T06:12:45Z","content_type":"application/pdf","relation":"main_file","access_level":"open_access","checksum":"4ceba04a96a74f5092ec3ce2c579a0c7","file_id":"6411"}],"publication_status":"published","publication_identifier":{"eissn":["10974172"],"issn":["00928674"]},"project":[{"grant_number":"742985","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020","_id":"261099A6-B435-11E9-9278-68D0E5697425"}],"title":"Re-activation of stem cell pathways for pattern restoration in plant wound healing","article_processing_charge":"No","external_id":{"pmid":["31051107"],"isi":["000466843000015"]},"author":[{"last_name":"Marhavá","full_name":"Marhavá, Petra","first_name":"Petra","id":"44E59624-F248-11E8-B48F-1D18A9856A87"},{"id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87","first_name":"Lukas","last_name":"Hörmayer","orcid":"0000-0001-8295-2926","full_name":"Hörmayer, Lukas"},{"first_name":"Saiko","id":"2E46069C-F248-11E8-B48F-1D18A9856A87","full_name":"Yoshida, Saiko","last_name":"Yoshida"},{"last_name":"Marhavy","orcid":"0000-0001-5227-5741","full_name":"Marhavy, Peter","first_name":"Peter","id":"3F45B078-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Benková","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"ista":"Marhavá P, Hörmayer L, Yoshida S, Marhavý P, Benková E, Friml J. 2019. Re-activation of stem cell pathways for pattern restoration in plant wound healing. Cell. 177(4), 957–969.e13.","chicago":"Marhavá, Petra, Lukas Hörmayer, Saiko Yoshida, Peter Marhavý, Eva Benková, and Jiří Friml. “Re-Activation of Stem Cell Pathways for Pattern Restoration in Plant Wound Healing.” Cell. Elsevier, 2019. https://doi.org/10.1016/j.cell.2019.04.015.","ieee":"P. Marhavá, L. Hörmayer, S. Yoshida, P. Marhavý, E. Benková, and J. Friml, “Re-activation of stem cell pathways for pattern restoration in plant wound healing,” Cell, vol. 177, no. 4. Elsevier, p. 957–969.e13, 2019.","short":"P. Marhavá, L. Hörmayer, S. Yoshida, P. Marhavý, E. Benková, J. Friml, Cell 177 (2019) 957–969.e13.","ama":"Marhavá P, Hörmayer L, Yoshida S, Marhavý P, Benková E, Friml J. Re-activation of stem cell pathways for pattern restoration in plant wound healing. Cell. 2019;177(4):957-969.e13. doi:10.1016/j.cell.2019.04.015","apa":"Marhavá, P., Hörmayer, L., Yoshida, S., Marhavý, P., Benková, E., & Friml, J. (2019). Re-activation of stem cell pathways for pattern restoration in plant wound healing. Cell. Elsevier. https://doi.org/10.1016/j.cell.2019.04.015","mla":"Marhavá, Petra, et al. “Re-Activation of Stem Cell Pathways for Pattern Restoration in Plant Wound Healing.” Cell, vol. 177, no. 4, Elsevier, 2019, p. 957–969.e13, doi:10.1016/j.cell.2019.04.015."},"oa":1,"quality_controlled":"1","publisher":"Elsevier","date_created":"2019-04-28T21:59:14Z","doi":"10.1016/j.cell.2019.04.015","date_published":"2019-05-02T00:00:00Z","page":"957-969.e13","publication":"Cell","day":"02","year":"2019","isi":1,"has_accepted_license":"1"},{"date_created":"2018-12-11T11:52:20Z","doi":"10.1101/gad.276964.115","date_published":"2016-03-01T00:00:00Z","page":"471 - 483","publication":"Genes and Development","day":"01","year":"2016","has_accepted_license":"1","oa":1,"quality_controlled":"1","publisher":"Cold Spring Harbor Laboratory Press","acknowledgement":"This work was supported by a European Research Council Starting Inde-pendent Research grant (ERC-2007-Stg-207362-HCPO to J.D.), Research Foundation-Flanders (G033711N to A.A.), and the Austrian Science Fund (FWF01_I1774S to E.B.). P.M. is indebted to the Federation of European Biochemical Sciences for a Long-Term Fellowship. ","title":"Targeted cell elimination reveals an auxin-guided biphasic mode of lateral root initiation","external_id":{"pmid":[" 26883363"]},"author":[{"last_name":"Marhavy","full_name":"Marhavy, Peter","orcid":"0000-0001-5227-5741","id":"3F45B078-F248-11E8-B48F-1D18A9856A87","first_name":"Peter"},{"last_name":"Montesinos López","orcid":"0000-0001-9179-6099","full_name":"Montesinos López, Juan C","first_name":"Juan C","id":"310A8E3E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Anas","last_name":"Abuzeineh","full_name":"Abuzeineh, Anas"},{"full_name":"Van Damme, Daniël","last_name":"Van Damme","first_name":"Daniël"},{"last_name":"Vermeer","full_name":"Vermeer, Joop","first_name":"Joop"},{"last_name":"Duclercq","full_name":"Duclercq, Jérôme","first_name":"Jérôme"},{"full_name":"Rakusova, Hana","last_name":"Rakusova","first_name":"Hana"},{"first_name":"Petra","id":"44E59624-F248-11E8-B48F-1D18A9856A87","last_name":"Marhavá","full_name":"Marhavá, Petra"},{"last_name":"Friml","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Geldner","full_name":"Geldner, Niko","first_name":"Niko"},{"orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","last_name":"Benková","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"}],"publist_id":"5691","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"ieee":"P. Marhavý et al., “Targeted cell elimination reveals an auxin-guided biphasic mode of lateral root initiation,” Genes and Development, vol. 30, no. 4. Cold Spring Harbor Laboratory Press, pp. 471–483, 2016.","short":"P. Marhavý, J.C. Montesinos López, A. Abuzeineh, D. Van Damme, J. Vermeer, J. Duclercq, H. Rakusova, P. Marhavá, J. Friml, N. Geldner, E. Benková, Genes and Development 30 (2016) 471–483.","apa":"Marhavý, P., Montesinos López, J. C., Abuzeineh, A., Van Damme, D., Vermeer, J., Duclercq, J., … Benková, E. (2016). Targeted cell elimination reveals an auxin-guided biphasic mode of lateral root initiation. Genes and Development. Cold Spring Harbor Laboratory Press. https://doi.org/10.1101/gad.276964.115","ama":"Marhavý P, Montesinos López JC, Abuzeineh A, et al. Targeted cell elimination reveals an auxin-guided biphasic mode of lateral root initiation. Genes and Development. 2016;30(4):471-483. doi:10.1101/gad.276964.115","mla":"Marhavý, Peter, et al. “Targeted Cell Elimination Reveals an Auxin-Guided Biphasic Mode of Lateral Root Initiation.” Genes and Development, vol. 30, no. 4, Cold Spring Harbor Laboratory Press, 2016, pp. 471–83, doi:10.1101/gad.276964.115.","ista":"Marhavý P, Montesinos López JC, Abuzeineh A, Van Damme D, Vermeer J, Duclercq J, Rakusova H, Marhavá P, Friml J, Geldner N, Benková E. 2016. Targeted cell elimination reveals an auxin-guided biphasic mode of lateral root initiation. Genes and Development. 30(4), 471–483.","chicago":"Marhavý, Peter, Juan C Montesinos López, Anas Abuzeineh, Daniël Van Damme, Joop Vermeer, Jérôme Duclercq, Hana Rakusova, et al. “Targeted Cell Elimination Reveals an Auxin-Guided Biphasic Mode of Lateral Root Initiation.” Genes and Development. Cold Spring Harbor Laboratory Press, 2016. https://doi.org/10.1101/gad.276964.115."},"license":"https://creativecommons.org/licenses/by-nc/4.0/","volume":30,"issue":"4","language":[{"iso":"eng"}],"file":[{"file_size":2757636,"date_updated":"2020-07-14T12:44:58Z","creator":"kschuh","file_name":"2016_GeneDev_Marhavy.pdf","date_created":"2019-01-25T09:56:11Z","content_type":"application/pdf","relation":"main_file","access_level":"open_access","checksum":"ea394498ee56270e021d1028a29358a0","file_id":"5883"}],"publication_status":"published","intvolume":" 30","month":"03","scopus_import":1,"oa_version":"Published Version","pmid":1,"acknowledged_ssus":[{"_id":"LifeSc"}],"abstract":[{"lang":"eng","text":"To sustain a lifelong ability to initiate organs, plants retain pools of undifferentiated cells with a preserved prolif eration capacity. The root pericycle represents a unique tissue with conditional meristematic activity, and its tight control determines initiation of lateral organs. Here we show that the meristematic activity of the pericycle is constrained by the interaction with the adjacent endodermis. Release of these restraints by elimination of endo dermal cells by single-cell ablation triggers the pericycle to re-enter the cell cycle. We found that endodermis removal substitutes for the phytohormone auxin-dependent initiation of the pericycle meristematic activity. However, auxin is indispensable to steer the cell division plane orientation of new organ-defining divisions. We propose a dual, spatiotemporally distinct role for auxin during lateral root initiation. In the endodermis, auxin releases constraints arising from cell-to-cell interactions that compromise the pericycle meristematic activity, whereas, in the pericycle, auxin defines the orientation of the cell division plane to initiate lateral roots."}],"file_date_updated":"2020-07-14T12:44:58Z","department":[{"_id":"EvBe"}],"ddc":["570"],"date_updated":"2021-01-12T06:51:08Z","status":"public","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","image":"/images/cc_by_nc.png","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","short":"CC BY-NC (4.0)"},"type":"journal_article","_id":"1492"},{"article_number":"8821","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"chicago":"Chen, Qian, Yang Liu, Steven Maere, Eunkyoung Lee, Gert Van Isterdael, Zidian Xie, Wei Xuan, et al. “A Coherent Transcriptional Feed-Forward Motif Model for Mediating Auxin-Sensitive PIN3 Expression during Lateral Root Development.” Nature Communications. Nature Publishing Group, 2015. https://doi.org/10.1038/ncomms9821.","ista":"Chen Q, Liu Y, Maere S, Lee E, Van Isterdael G, Xie Z, Xuan W, Lucas J, Vassileva V, Kitakura S, Marhavý P, Wabnik KT, Geldner N, Benková E, Le J, Fukaki H, Grotewold E, Li C, Friml J, Sack F, Beeckman T, Vanneste S. 2015. A coherent transcriptional feed-forward motif model for mediating auxin-sensitive PIN3 expression during lateral root development. Nature Communications. 6, 8821.","mla":"Chen, Qian, et al. “A Coherent Transcriptional Feed-Forward Motif Model for Mediating Auxin-Sensitive PIN3 Expression during Lateral Root Development.” Nature Communications, vol. 6, 8821, Nature Publishing Group, 2015, doi:10.1038/ncomms9821.","ieee":"Q. Chen et al., “A coherent transcriptional feed-forward motif model for mediating auxin-sensitive PIN3 expression during lateral root development,” Nature Communications, vol. 6. Nature Publishing Group, 2015.","short":"Q. Chen, Y. Liu, S. Maere, E. Lee, G. Van Isterdael, Z. Xie, W. Xuan, J. Lucas, V. Vassileva, S. Kitakura, P. Marhavý, K.T. Wabnik, N. Geldner, E. Benková, J. Le, H. Fukaki, E. Grotewold, C. Li, J. Friml, F. Sack, T. Beeckman, S. Vanneste, Nature Communications 6 (2015).","ama":"Chen Q, Liu Y, Maere S, et al. A coherent transcriptional feed-forward motif model for mediating auxin-sensitive PIN3 expression during lateral root development. Nature Communications. 2015;6. doi:10.1038/ncomms9821","apa":"Chen, Q., Liu, Y., Maere, S., Lee, E., Van Isterdael, G., Xie, Z., … Vanneste, S. (2015). A coherent transcriptional feed-forward motif model for mediating auxin-sensitive PIN3 expression during lateral root development. Nature Communications. Nature Publishing Group. https://doi.org/10.1038/ncomms9821"},"title":"A coherent transcriptional feed-forward motif model for mediating auxin-sensitive PIN3 expression during lateral root development","publist_id":"5597","author":[{"first_name":"Qian","full_name":"Chen, Qian","last_name":"Chen"},{"last_name":"Liu","full_name":"Liu, Yang","first_name":"Yang"},{"last_name":"Maere","full_name":"Maere, Steven","first_name":"Steven"},{"first_name":"Eunkyoung","last_name":"Lee","full_name":"Lee, Eunkyoung"},{"full_name":"Van Isterdael, Gert","last_name":"Van Isterdael","first_name":"Gert"},{"last_name":"Xie","full_name":"Xie, Zidian","first_name":"Zidian"},{"full_name":"Xuan, Wei","last_name":"Xuan","first_name":"Wei"},{"first_name":"Jessica","last_name":"Lucas","full_name":"Lucas, Jessica"},{"last_name":"Vassileva","full_name":"Vassileva, Valya","first_name":"Valya"},{"first_name":"Saeko","full_name":"Kitakura, Saeko","last_name":"Kitakura"},{"id":"3F45B078-F248-11E8-B48F-1D18A9856A87","first_name":"Peter","last_name":"Marhavy","orcid":"0000-0001-5227-5741","full_name":"Marhavy, Peter"},{"first_name":"Krzysztof T","id":"4DE369A4-F248-11E8-B48F-1D18A9856A87","last_name":"Wabnik","full_name":"Wabnik, Krzysztof T","orcid":"0000-0001-7263-0560"},{"full_name":"Geldner, Niko","last_name":"Geldner","first_name":"Niko"},{"last_name":"Benková","full_name":"Benková, Eva","orcid":"0000-0002-8510-9739","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Le","full_name":"Le, Jie","first_name":"Jie"},{"first_name":"Hidehiro","full_name":"Fukaki, Hidehiro","last_name":"Fukaki"},{"first_name":"Erich","full_name":"Grotewold, Erich","last_name":"Grotewold"},{"first_name":"Chuanyou","full_name":"Li, Chuanyou","last_name":"Li"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí"},{"first_name":"Fred","full_name":"Sack, Fred","last_name":"Sack"},{"full_name":"Beeckman, Tom","last_name":"Beeckman","first_name":"Tom"},{"full_name":"Vanneste, Steffen","last_name":"Vanneste","first_name":"Steffen"}],"acknowledgement":"of the European Research Council (project ERC-2011-StG-20101109-PSDP) (to J.F.), a FEBS long-term fellowship (to P.M.) ","oa":1,"quality_controlled":"1","publisher":"Nature Publishing Group","publication":"Nature Communications","day":"18","year":"2015","has_accepted_license":"1","date_created":"2018-12-11T11:52:48Z","date_published":"2015-11-18T00:00:00Z","doi":"10.1038/ncomms9821","_id":"1574","pubrep_id":"477","status":"public","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"type":"journal_article","ddc":["580"],"date_updated":"2021-01-12T06:51:42Z","department":[{"_id":"EvBe"},{"_id":"JiFr"}],"file_date_updated":"2020-07-14T12:45:02Z","oa_version":"Published Version","abstract":[{"lang":"eng","text":"Multiple plant developmental processes, such as lateral root development, depend on auxin distribution patterns that are in part generated by the PIN-formed family of auxin-efflux transporters. Here we propose that AUXIN RESPONSE FACTOR7 (ARF7) and the ARF7-regulated FOUR LIPS/MYB124 (FLP) transcription factors jointly form a coherent feed-forward motif that mediates the auxin-responsive PIN3 transcription in planta to steer the early steps of lateral root formation. This regulatory mechanism might endow the PIN3 circuitry with a temporal 'memory' of auxin stimuli, potentially maintaining and enhancing the robustness of the auxin flux directionality during lateral root development. The cooperative action between canonical auxin signalling and other transcription factors might constitute a general mechanism by which transcriptional auxin-sensitivity can be regulated at a tissue-specific level."}],"intvolume":" 6","month":"11","scopus_import":1,"language":[{"iso":"eng"}],"file":[{"content_type":"application/pdf","access_level":"open_access","relation":"main_file","checksum":"8ff5c108899b548806e1cb7a302fe76d","file_id":"5085","date_updated":"2020-07-14T12:45:02Z","file_size":1701815,"creator":"system","date_created":"2018-12-12T10:14:32Z","file_name":"IST-2016-477-v1+1_ncomms9821.pdf"}],"publication_status":"published","volume":6},{"ddc":["580"],"date_updated":"2021-01-12T06:52:11Z","file_date_updated":"2020-07-14T12:45:08Z","department":[{"_id":"EvBe"},{"_id":"JiFr"}],"_id":"1640","pubrep_id":"1020","status":"public","type":"journal_article","language":[{"iso":"eng"}],"file":[{"date_created":"2018-12-12T10:18:36Z","file_name":"IST-2018-1020-v1+1_Simaskova_et_al_NatCom_2015.pdf","creator":"system","date_updated":"2020-07-14T12:45:08Z","file_size":1471217,"file_id":"5358","checksum":"c2c84bca37401435fedf76bad0ba0579","access_level":"open_access","relation":"main_file","content_type":"application/pdf"}],"publication_status":"published","ec_funded":1,"volume":6,"oa_version":"Submitted Version","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"abstract":[{"lang":"eng","text":"Auxin and cytokinin are key endogenous regulators of plant development. Although cytokinin-mediated modulation of auxin distribution is a developmentally crucial hormonal interaction, its molecular basis is largely unknown. Here we show a direct regulatory link between cytokinin signalling and the auxin transport machinery uncovering a mechanistic framework for cytokinin-auxin cross-talk. We show that the CYTOKININ RESPONSE FACTORS (CRFs), transcription factors downstream of cytokinin perception, transcriptionally control genes encoding PIN-FORMED (PIN) auxin transporters at a specific PIN CYTOKININ RESPONSE ELEMENT (PCRE) domain. Removal of this cis-regulatory element effectively uncouples PIN transcription from the CRF-mediated cytokinin regulation and attenuates plant cytokinin sensitivity. We propose that CRFs represent a missing cross-talk component that fine-tunes auxin transport capacity downstream of cytokinin signalling to control plant development."}],"intvolume":" 6","month":"01","scopus_import":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"apa":"Šimášková, M., O’Brien, J., Khan-Djamei, M., Van Noorden, G., Ötvös, K., Vieten, A., … Benková, E. (2015). Cytokinin response factors regulate PIN-FORMED auxin transporters. Nature Communications. Nature Publishing Group. https://doi.org/10.1038/ncomms9717","ama":"Šimášková M, O’Brien J, Khan-Djamei M, et al. Cytokinin response factors regulate PIN-FORMED auxin transporters. Nature Communications. 2015;6. doi:10.1038/ncomms9717","short":"M. Šimášková, J. O’Brien, M. Khan-Djamei, G. Van Noorden, K. Ötvös, A. Vieten, I. De Clercq, J. Van Haperen, C. Cuesta, K. Hoyerová, S. Vanneste, P. Marhavý, K.T. Wabnik, F. Van Breusegem, M. Nowack, A. Murphy, J. Friml, D. Weijers, T. Beeckman, E. Benková, Nature Communications 6 (2015).","ieee":"M. Šimášková et al., “Cytokinin response factors regulate PIN-FORMED auxin transporters,” Nature Communications, vol. 6. Nature Publishing Group, 2015.","mla":"Šimášková, Mária, et al. “Cytokinin Response Factors Regulate PIN-FORMED Auxin Transporters.” Nature Communications, vol. 6, 8717, Nature Publishing Group, 2015, doi:10.1038/ncomms9717.","ista":"Šimášková M, O’Brien J, Khan-Djamei M, Van Noorden G, Ötvös K, Vieten A, De Clercq I, Van Haperen J, Cuesta C, Hoyerová K, Vanneste S, Marhavý P, Wabnik KT, Van Breusegem F, Nowack M, Murphy A, Friml J, Weijers D, Beeckman T, Benková E. 2015. Cytokinin response factors regulate PIN-FORMED auxin transporters. Nature Communications. 6, 8717.","chicago":"Šimášková, Mária, José O’Brien, Mamoona Khan-Djamei, Giel Van Noorden, Krisztina Ötvös, Anne Vieten, Inge De Clercq, et al. “Cytokinin Response Factors Regulate PIN-FORMED Auxin Transporters.” Nature Communications. Nature Publishing Group, 2015. https://doi.org/10.1038/ncomms9717."},"title":"Cytokinin response factors regulate PIN-FORMED auxin transporters","publist_id":"5513","author":[{"first_name":"Mária","full_name":"Šimášková, Mária","last_name":"Šimášková"},{"first_name":"José","full_name":"O'Brien, José","last_name":"O'Brien"},{"last_name":"Khan-Djamei","full_name":"Khan-Djamei, Mamoona","first_name":"Mamoona","id":"391B5BBC-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Giel","last_name":"Van Noorden","full_name":"Van Noorden, Giel"},{"id":"29B901B0-F248-11E8-B48F-1D18A9856A87","first_name":"Krisztina","full_name":"Ötvös, Krisztina","orcid":"0000-0002-5503-4983","last_name":"Ötvös"},{"last_name":"Vieten","full_name":"Vieten, Anne","first_name":"Anne"},{"full_name":"De Clercq, Inge","last_name":"De Clercq","first_name":"Inge"},{"full_name":"Van Haperen, Johanna","last_name":"Van Haperen","first_name":"Johanna"},{"first_name":"Candela","id":"33A3C818-F248-11E8-B48F-1D18A9856A87","full_name":"Cuesta, Candela","orcid":"0000-0003-1923-2410","last_name":"Cuesta"},{"full_name":"Hoyerová, Klára","last_name":"Hoyerová","first_name":"Klára"},{"first_name":"Steffen","full_name":"Vanneste, Steffen","last_name":"Vanneste"},{"last_name":"Marhavy","orcid":"0000-0001-5227-5741","full_name":"Marhavy, Peter","id":"3F45B078-F248-11E8-B48F-1D18A9856A87","first_name":"Peter"},{"orcid":"0000-0001-7263-0560","full_name":"Wabnik, Krzysztof T","last_name":"Wabnik","id":"4DE369A4-F248-11E8-B48F-1D18A9856A87","first_name":"Krzysztof T"},{"first_name":"Frank","full_name":"Van Breusegem, Frank","last_name":"Van Breusegem"},{"last_name":"Nowack","full_name":"Nowack, Moritz","first_name":"Moritz"},{"first_name":"Angus","last_name":"Murphy","full_name":"Murphy, Angus"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiřĺ","last_name":"Friml","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiřĺ"},{"first_name":"Dolf","last_name":"Weijers","full_name":"Weijers, Dolf"},{"full_name":"Beeckman, Tom","last_name":"Beeckman","first_name":"Tom"},{"last_name":"Benková","full_name":"Benková, Eva","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva"}],"article_number":"8717","project":[{"name":"Hormonal cross-talk in plant organogenesis","grant_number":"207362","call_identifier":"FP7","_id":"253FCA6A-B435-11E9-9278-68D0E5697425"},{"_id":"2542D156-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Hormone cross-talk drives nutrient dependent plant development","grant_number":"I 1774-B16"}],"publication":"Nature Communications","day":"01","year":"2015","has_accepted_license":"1","date_created":"2018-12-11T11:53:12Z","date_published":"2015-01-01T00:00:00Z","doi":"10.1038/ncomms9717","acknowledgement":"This work was supported by the European Research Council Starting Independent Research grant (ERC-2007-Stg-207362-HCPO to E.B., M.S., C.C.), by the Ghent University Multidisciplinary Research Partnership ‘Biotechnology for a Sustainable Economy’ no.01MRB510W, by the Research Foundation—Flanders (grant 3G033711 to J.-A.O.), by the Austrian Science Fund (FWF01_I1774S) to K.Ö.,E.B., and by the Interuniversity Attraction Poles Programme (IUAP P7/29 ‘MARS’) initiated by the Belgian Science Policy Office. I.D.C. and S.V. are post-doctoral fellows of the Research Foundation—Flanders (FWO). This research was supported by the Scientific Service Units (SSU) of IST-Austria through resources provided by the Bioimaging Facility (BIF), the Life Science Facility (LSF).","oa":1,"publisher":"Nature Publishing Group","quality_controlled":"1"},{"acknowledgement":"European Research Council with a Starting Independent Research grant: ERC-2007-Stg-207362-HCPO, Czech Science Foundation: GA13-39982S\nWe thank Matyas Fendrych for critical reading and comments. The protocol was developed based on previously published work of De Rybel et al. (2010) and Laskowski et al. (2008). ","abstract":[{"lang":"eng","text":"Plants maintain capacity to form new organs such as leaves, flowers, lateral shoots and roots throughout their postembryonic lifetime. Lateral roots (LRs) originate from a few pericycle cells that acquire attributes of founder cells (FCs), undergo series of anticlinal divisions, and give rise to a few short initial cells. After initiation, coordinated cell division and differentiation occur, giving rise to lateral root primordia (LRP). Primordia continue to grow, emerge through the cortex and epidermal layers of the primary root, and finally a new apical meristem is established taking over the responsibility for growth of mature lateral roots [for detailed description of the individual stages of lateral root organogenesis see Malamy and Benfey (1997)]. To examine this highly dynamic developmental process and to investigate a role of various hormonal, genetic and environmental factors in the regulation of lateral root organogenesis, the real time imaging based analyses represent extremely powerful tools (Laskowski et al., 2008; De Smet et al., 2012; Marhavy et al., 2013 and 2014). Herein, we describe a protocol for real time lateral root primordia (LRP) analysis, which enables the monitoring of an onset of the specific gene expression and subcellular protein localization during primordia organogenesis, as well as the evaluation of the impact of genetic and environmental perturbations on LRP organogenesis."}],"intvolume":" 5","month":"04","publisher":"Bio-protocol LLC","quality_controlled":0,"publication":"Bio-protocol","day":"20","year":"2015","publication_status":"published","date_created":"2018-12-11T11:48:44Z","volume":5,"doi":"10.21769/BioProtoc.1446","date_published":"2015-04-20T00:00:00Z","issue":"8","_id":"832","status":"public","type":"journal_article","extern":1,"date_updated":"2021-01-12T08:18:07Z","citation":{"ista":"Marhavý P, Benková E. 2015. Real time analysis of lateral root organogenesis in arabidopsis. Bio-protocol. 5(8).","chicago":"Marhavý, Peter, and Eva Benková. “Real Time Analysis of Lateral Root Organogenesis in Arabidopsis.” Bio-Protocol. Bio-protocol LLC, 2015. https://doi.org/10.21769/BioProtoc.1446.","ieee":"P. Marhavý and E. Benková, “Real time analysis of lateral root organogenesis in arabidopsis,” Bio-protocol, vol. 5, no. 8. Bio-protocol LLC, 2015.","short":"P. Marhavý, E. Benková, Bio-Protocol 5 (2015).","apa":"Marhavý, P., & Benková, E. (2015). Real time analysis of lateral root organogenesis in arabidopsis. Bio-Protocol. Bio-protocol LLC. https://doi.org/10.21769/BioProtoc.1446","ama":"Marhavý P, Benková E. Real time analysis of lateral root organogenesis in arabidopsis. Bio-protocol. 2015;5(8). doi:10.21769/BioProtoc.1446","mla":"Marhavý, Peter, and Eva Benková. “Real Time Analysis of Lateral Root Organogenesis in Arabidopsis.” Bio-Protocol, vol. 5, no. 8, Bio-protocol LLC, 2015, doi:10.21769/BioProtoc.1446."},"title":"Real time analysis of lateral root organogenesis in arabidopsis","publist_id":"6816","author":[{"full_name":"Peter Marhavy","orcid":"0000-0001-5227-5741","last_name":"Marhavy","first_name":"Peter","id":"3F45B078-F248-11E8-B48F-1D18A9856A87"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","last_name":"Benková","full_name":"Eva Benková","orcid":"0000-0002-8510-9739"}]},{"publist_id":"5160","author":[{"id":"3F45B078-F248-11E8-B48F-1D18A9856A87","first_name":"Peter","orcid":"0000-0001-5227-5741","full_name":"Marhavy, Peter","last_name":"Marhavy"},{"full_name":"Duclercq, Jérôme","last_name":"Duclercq","first_name":"Jérôme"},{"full_name":"Weller, Benjamin","last_name":"Weller","first_name":"Benjamin"},{"last_name":"Feraru","full_name":"Feraru, Elena","first_name":"Elena"},{"first_name":"Agnieszka","full_name":"Bielach, Agnieszka","last_name":"Bielach"},{"last_name":"Offringa","full_name":"Offringa, Remko","first_name":"Remko"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí"},{"full_name":"Schwechheimer, Claus","last_name":"Schwechheimer","first_name":"Claus"},{"first_name":"Angus","last_name":"Murphy","full_name":"Murphy, Angus"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","last_name":"Benková","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva"}],"title":"Cytokinin controls polarity of PIN1-dependent Auxin transport during lateral root organogenesis","department":[{"_id":"EvBe"},{"_id":"JiFr"}],"date_updated":"2021-01-12T06:54:10Z","citation":{"mla":"Marhavý, Peter, et al. “Cytokinin Controls Polarity of PIN1-Dependent Auxin Transport during Lateral Root Organogenesis.” Current Biology, vol. 24, no. 9, Cell Press, 2014, pp. 1031–37, doi:10.1016/j.cub.2014.04.002.","short":"P. Marhavý, J. Duclercq, B. Weller, E. Feraru, A. Bielach, R. Offringa, J. Friml, C. Schwechheimer, A. Murphy, E. Benková, Current Biology 24 (2014) 1031–1037.","ieee":"P. Marhavý et al., “Cytokinin controls polarity of PIN1-dependent Auxin transport during lateral root organogenesis,” Current Biology, vol. 24, no. 9. Cell Press, pp. 1031–1037, 2014.","apa":"Marhavý, P., Duclercq, J., Weller, B., Feraru, E., Bielach, A., Offringa, R., … Benková, E. (2014). Cytokinin controls polarity of PIN1-dependent Auxin transport during lateral root organogenesis. Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2014.04.002","ama":"Marhavý P, Duclercq J, Weller B, et al. Cytokinin controls polarity of PIN1-dependent Auxin transport during lateral root organogenesis. Current Biology. 2014;24(9):1031-1037. doi:10.1016/j.cub.2014.04.002","chicago":"Marhavý, Peter, Jérôme Duclercq, Benjamin Weller, Elena Feraru, Agnieszka Bielach, Remko Offringa, Jiří Friml, Claus Schwechheimer, Angus Murphy, and Eva Benková. “Cytokinin Controls Polarity of PIN1-Dependent Auxin Transport during Lateral Root Organogenesis.” Current Biology. Cell Press, 2014. https://doi.org/10.1016/j.cub.2014.04.002.","ista":"Marhavý P, Duclercq J, Weller B, Feraru E, Bielach A, Offringa R, Friml J, Schwechheimer C, Murphy A, Benková E. 2014. Cytokinin controls polarity of PIN1-dependent Auxin transport during lateral root organogenesis. Current Biology. 24(9), 1031–1037."},"user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","type":"journal_article","project":[{"grant_number":"207362","name":"Hormonal cross-talk in plant organogenesis","_id":"253FCA6A-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"}],"status":"public","_id":"1934","page":"1031 - 1037","volume":24,"date_published":"2014-05-05T00:00:00Z","issue":"9","doi":"10.1016/j.cub.2014.04.002","ec_funded":1,"date_created":"2018-12-11T11:54:48Z","year":"2014","publication_status":"published","day":"05","language":[{"iso":"eng"}],"publication":"Current Biology","publisher":"Cell Press","scopus_import":1,"quality_controlled":"1","month":"05","intvolume":" 24","abstract":[{"text":"The plant hormones auxin and cytokinin mutually coordinate their activities to control various aspects of development [1-9], and their crosstalk occurs at multiple levels [10, 11]. Cytokinin-mediated modulation of auxin transport provides an efficient means to regulate auxin distribution in plant organs. Here, we demonstrate that cytokinin does not merely control the overall auxin flow capacity, but might also act as a polarizing cue and control the auxin stream directionality during plant organogenesis. Cytokinin enhances the PIN-FORMED1 (PIN1) auxin transporter depletion at specific polar domains, thus rearranging the cellular PIN polarities and directly regulating the auxin flow direction. This selective cytokinin sensitivity correlates with the PIN protein phosphorylation degree. PIN1 phosphomimicking mutations, as well as enhanced phosphorylation in plants with modulated activities of PIN-specific kinases and phosphatases, desensitize PIN1 to cytokinin. Our results reveal conceptually novel, cytokinin-driven polarization mechanism that operates in developmental processes involving rapid auxin stream redirection, such as lateral root organogenesis, in which a gradual PIN polarity switch defines the growth axis of the newly formed organ.","lang":"eng"}],"oa_version":"None"},{"date_published":"2013-05-06T00:00:00Z","volume":23,"issue":"9","doi":"10.1016/j.cub.2013.03.064","date_created":"2018-12-11T11:59:53Z","ec_funded":1,"page":"817 - 822","day":"06","language":[{"iso":"eng"}],"publication":"Current Biology","year":"2013","publication_status":"published","month":"05","intvolume":" 23","quality_controlled":"1","publisher":"Cell Press","scopus_import":1,"oa_version":"None","abstract":[{"lang":"eng","text":"As soon as a seed germinates, plant growth relates to gravity to ensure that the root penetrates the soil and the shoot expands aerially. Whereas mechanisms of positive and negative orthogravitropism of primary roots and shoots are relatively well understood [1-3], lateral organs often show more complex growth behavior [4]. Lateral roots (LRs) seemingly suppress positive gravitropic growth and show a defined gravitropic set-point angle (GSA) that allows radial expansion of the root system (plagiotropism) [3, 4]. Despite its eminent importance for root architecture, it so far remains completely unknown how lateral organs partially suppress positive orthogravitropism. Here we show that the phytohormone auxin steers GSA formation and limits positive orthogravitropism in LR. Low and high auxin levels/signaling lead to radial or axial root systems, respectively. At a cellular level, it is the auxin transport-dependent regulation of asymmetric growth in the elongation zone that determines GSA. Our data suggest that strong repression of PIN4/PIN7 and transient PIN3 expression limit auxin redistribution in young LR columella cells. We conclude that PIN activity, by temporally limiting the asymmetric auxin fluxes in the tip of LRs, induces transient, differential growth responses in the elongation zone and, consequently, controls root architecture."}],"department":[{"_id":"JiFr"},{"_id":"EvBe"}],"title":"An auxin transport mechanism restricts positive orthogravitropism in lateral roots","publist_id":"3950","author":[{"full_name":"Rosquete, Michel","last_name":"Rosquete","first_name":"Michel"},{"orcid":"0000-0002-6862-1247","full_name":"Von Wangenheim, Daniel","last_name":"Von Wangenheim","id":"49E91952-F248-11E8-B48F-1D18A9856A87","first_name":"Daniel"},{"last_name":"Marhavy","full_name":"Marhavy, Peter","orcid":"0000-0001-5227-5741","first_name":"Peter","id":"3F45B078-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Barbez","full_name":"Barbez, Elke","first_name":"Elke"},{"first_name":"Ernst","last_name":"Stelzer","full_name":"Stelzer, Ernst"},{"last_name":"Benková","full_name":"Benková, Eva","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva"},{"first_name":"Alexis","last_name":"Maizel","full_name":"Maizel, Alexis"},{"last_name":"Kleine Vehn","full_name":"Kleine Vehn, Jürgen","first_name":"Jürgen"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"ista":"Rosquete M, von Wangenheim D, Marhavý P, Barbez E, Stelzer E, Benková E, Maizel A, Kleine Vehn J. 2013. An auxin transport mechanism restricts positive orthogravitropism in lateral roots. Current Biology. 23(9), 817–822.","chicago":"Rosquete, Michel, Daniel von Wangenheim, Peter Marhavý, Elke Barbez, Ernst Stelzer, Eva Benková, Alexis Maizel, and Jürgen Kleine Vehn. “An Auxin Transport Mechanism Restricts Positive Orthogravitropism in Lateral Roots.” Current Biology. Cell Press, 2013. https://doi.org/10.1016/j.cub.2013.03.064.","apa":"Rosquete, M., von Wangenheim, D., Marhavý, P., Barbez, E., Stelzer, E., Benková, E., … Kleine Vehn, J. (2013). An auxin transport mechanism restricts positive orthogravitropism in lateral roots. Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2013.03.064","ama":"Rosquete M, von Wangenheim D, Marhavý P, et al. An auxin transport mechanism restricts positive orthogravitropism in lateral roots. Current Biology. 2013;23(9):817-822. doi:10.1016/j.cub.2013.03.064","short":"M. Rosquete, D. von Wangenheim, P. Marhavý, E. Barbez, E. Stelzer, E. Benková, A. Maizel, J. Kleine Vehn, Current Biology 23 (2013) 817–822.","ieee":"M. Rosquete et al., “An auxin transport mechanism restricts positive orthogravitropism in lateral roots,” Current Biology, vol. 23, no. 9. Cell Press, pp. 817–822, 2013.","mla":"Rosquete, Michel, et al. “An Auxin Transport Mechanism Restricts Positive Orthogravitropism in Lateral Roots.” Current Biology, vol. 23, no. 9, Cell Press, 2013, pp. 817–22, doi:10.1016/j.cub.2013.03.064."},"date_updated":"2021-01-12T07:00:10Z","status":"public","project":[{"grant_number":"207362","name":"Hormonal cross-talk in plant organogenesis","call_identifier":"FP7","_id":"253FCA6A-B435-11E9-9278-68D0E5697425"}],"type":"journal_article","_id":"2844"},{"oa":1,"quality_controlled":"1","publisher":"Wiley-Blackwell","publication":"EMBO Journal","day":"09","year":"2013","date_created":"2018-12-11T12:00:07Z","doi":"10.1038/emboj.2012.303","date_published":"2013-01-09T00:00:00Z","page":"149 - 158","project":[{"_id":"253FCA6A-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","grant_number":"207362","name":"Hormonal cross-talk in plant organogenesis"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"mla":"Marhavý, Peter, et al. “Auxin Reflux between the Endodermis and Pericycle Promotes Lateral Root Initiation.” EMBO Journal, vol. 32, no. 1, Wiley-Blackwell, 2013, pp. 149–58, doi:10.1038/emboj.2012.303.","apa":"Marhavý, P., Vanstraelen, M., De Rybel, B., Zhaojun, D., Bennett, M., Beeckman, T., & Benková, E. (2013). Auxin reflux between the endodermis and pericycle promotes lateral root initiation. EMBO Journal. Wiley-Blackwell. https://doi.org/10.1038/emboj.2012.303","ama":"Marhavý P, Vanstraelen M, De Rybel B, et al. Auxin reflux between the endodermis and pericycle promotes lateral root initiation. EMBO Journal. 2013;32(1):149-158. doi:10.1038/emboj.2012.303","short":"P. Marhavý, M. Vanstraelen, B. De Rybel, D. Zhaojun, M. Bennett, T. Beeckman, E. Benková, EMBO Journal 32 (2013) 149–158.","ieee":"P. Marhavý et al., “Auxin reflux between the endodermis and pericycle promotes lateral root initiation,” EMBO Journal, vol. 32, no. 1. Wiley-Blackwell, pp. 149–158, 2013.","chicago":"Marhavý, Peter, Marleen Vanstraelen, Bert De Rybel, Ding Zhaojun, Malcolm Bennett, Tom Beeckman, and Eva Benková. “Auxin Reflux between the Endodermis and Pericycle Promotes Lateral Root Initiation.” EMBO Journal. Wiley-Blackwell, 2013. https://doi.org/10.1038/emboj.2012.303.","ista":"Marhavý P, Vanstraelen M, De Rybel B, Zhaojun D, Bennett M, Beeckman T, Benková E. 2013. Auxin reflux between the endodermis and pericycle promotes lateral root initiation. EMBO Journal. 32(1), 149–158."},"title":"Auxin reflux between the endodermis and pericycle promotes lateral root initiation","external_id":{"pmid":["23178590"]},"publist_id":"3882","author":[{"first_name":"Peter","id":"3F45B078-F248-11E8-B48F-1D18A9856A87","last_name":"Marhavy","full_name":"Marhavy, Peter","orcid":"0000-0001-5227-5741"},{"first_name":"Marleen","last_name":"Vanstraelen","full_name":"Vanstraelen, Marleen"},{"first_name":"Bert","full_name":"De Rybel, Bert","last_name":"De Rybel"},{"first_name":"Ding","last_name":"Zhaojun","full_name":"Zhaojun, Ding"},{"last_name":"Bennett","full_name":"Bennett, Malcolm","first_name":"Malcolm"},{"full_name":"Beeckman, Tom","last_name":"Beeckman","first_name":"Tom"},{"full_name":"Benková, Eva","orcid":"0000-0002-8510-9739","last_name":"Benková","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"}],"oa_version":"Submitted Version","pmid":1,"abstract":[{"text":"Lateral root (LR) formation is initiated when pericycle cells accumulate auxin, thereby acquiring founder cell (FC) status and triggering asymmetric cell divisions, giving rise to a new primordium. How this auxin maximum in pericycle cells builds up and remains focused is not understood. We report that the endodermis plays an active role in the regulation of auxin accumulation and is instructive for FCs to progress during the LR initiation (LRI) phase. We describe the functional importance of a PIN3 (PIN-formed) auxin efflux carrier-dependent hormone reflux pathway between overlaying endodermal and pericycle FCs. Disrupting this reflux pathway causes dramatic defects in the progress of FCs towards the next initiation phase. Our data identify an unexpected regulatory function for the endodermis in LRI as part of the fine-tuning mechanism that appears to act as a check point in LR organogenesis after FCs are specified.","lang":"eng"}],"intvolume":" 32","month":"01","main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3545298/"}],"scopus_import":1,"language":[{"iso":"eng"}],"publication_status":"published","ec_funded":1,"issue":"1","volume":32,"_id":"2880","status":"public","type":"journal_article","date_updated":"2021-01-12T07:00:27Z","department":[{"_id":"EvBe"}]},{"status":"public","type":"journal_article","_id":"2875","title":"Genetic approach towards the identification of auxin - cytokinin crosstalk components involved in root development","author":[{"first_name":"Agnieszka","last_name":"Bielach","full_name":"Bielach, Agnieszka"},{"full_name":"Duclercq, Jérôme","last_name":"Duclercq","first_name":"Jérôme"},{"first_name":"Peter","id":"3F45B078-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5227-5741","full_name":"Peter Marhavy","last_name":"Marhavy"},{"last_name":"Benková","orcid":"0000-0002-8510-9739","full_name":"Eva Benková","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"}],"publist_id":"3887","extern":1,"citation":{"ista":"Bielach A, Duclercq J, Marhavý P, Benková E. 2012. Genetic approach towards the identification of auxin - cytokinin crosstalk components involved in root development. Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences. 367(1595), 1469–1478.","chicago":"Bielach, Agnieszka, Jérôme Duclercq, Peter Marhavý, and Eva Benková. “Genetic Approach towards the Identification of Auxin - Cytokinin Crosstalk Components Involved in Root Development.” Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences. Royal Society, The, 2012. https://doi.org/10.1098/rstb.2011.0233.","short":"A. Bielach, J. Duclercq, P. Marhavý, E. Benková, Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences 367 (2012) 1469–1478.","ieee":"A. Bielach, J. Duclercq, P. Marhavý, and E. Benková, “Genetic approach towards the identification of auxin - cytokinin crosstalk components involved in root development,” Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences, vol. 367, no. 1595. Royal Society, The, pp. 1469–1478, 2012.","ama":"Bielach A, Duclercq J, Marhavý P, Benková E. Genetic approach towards the identification of auxin - cytokinin crosstalk components involved in root development. Philosophical Transactions of the Royal Society of London Series B, Biological Sciences. 2012;367(1595):1469-1478. doi:10.1098/rstb.2011.0233","apa":"Bielach, A., Duclercq, J., Marhavý, P., & Benková, E. (2012). Genetic approach towards the identification of auxin - cytokinin crosstalk components involved in root development. Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences. Royal Society, The. https://doi.org/10.1098/rstb.2011.0233","mla":"Bielach, Agnieszka, et al. “Genetic Approach towards the Identification of Auxin - Cytokinin Crosstalk Components Involved in Root Development.” Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences, vol. 367, no. 1595, Royal Society, The, 2012, pp. 1469–78, doi:10.1098/rstb.2011.0233."},"date_updated":"2021-01-12T07:00:25Z","intvolume":" 367","month":"06","oa":1,"main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3321684/","open_access":"1"}],"quality_controlled":0,"publisher":"Royal Society, The","abstract":[{"text":"Phytohormones are important plant growth regulators that control many developmental processes, such as cell division, cell differentiation, organogenesis and morphogenesis. They regulate a multitude of apparently unrelated physiological processes, often with overlapping roles, and they mutually modulate their effects. These features imply important synergistic and antagonistic interactions between the various plant hormones. Auxin and cytokinin are central hormones involved in the regulation of plant growth and development, including processes determining root architecture, such as root pole establishment during early embryogenesis, root meristem maintenance and lateral root organogenesis. Thus, to control root development both pathways put special demands on the mechanisms that balance their activities and mediate their interactions. Here, we summarize recent knowledge on the role of auxin and cytokinin in the regulation of root architecture with special focus on lateral root organogenesis, discuss the latest findings on the molecular mechanisms of their interactions, and present forward genetic screen as a tool to identify novel molecular components of the auxin and cytokinin crosstalk.","lang":"eng"}],"date_created":"2018-12-11T12:00:05Z","volume":367,"issue":"1595","doi":"10.1098/rstb.2011.0233","date_published":"2012-06-05T00:00:00Z","page":"1469 - 1478","publication":"Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences","day":"05","year":"2012","publication_status":"published"},{"publist_id":"6819","author":[{"first_name":"Agnieszka","full_name":"Bielach, Agnieszka","last_name":"Bielach"},{"first_name":"Katerina","last_name":"Podlesakova","full_name":"Podlesakova, Katerina"},{"first_name":"Peter","id":"3F45B078-F248-11E8-B48F-1D18A9856A87","full_name":"Peter Marhavy","orcid":"0000-0001-5227-5741","last_name":"Marhavy"},{"full_name":"Duclercq, Jérôme","last_name":"Duclercq","first_name":"Jérôme"},{"orcid":"0000-0003-1923-2410","full_name":"Candela Cuesta","last_name":"Cuesta","first_name":"Candela","id":"33A3C818-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Bruno","full_name":"Muller, Bruno","last_name":"Muller"},{"full_name":"Grunewald, Wim","last_name":"Grunewald","first_name":"Wim"},{"first_name":"Petr","last_name":"Tarkowski","full_name":"Tarkowski, Petr"},{"full_name":"Eva Benková","orcid":"0000-0002-8510-9739","last_name":"Benková","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"}],"title":"Spatiotemporal regulation of lateral root organogenesis in Arabidopsis by cytokinin","citation":{"short":"A. Bielach, K. Podlesakova, P. Marhavý, J. Duclercq, C. Cuesta, B. Muller, W. Grunewald, P. Tarkowski, E. Benková, The Plant Cell 24 (2012) 3967–3981.","ieee":"A. Bielach et al., “Spatiotemporal regulation of lateral root organogenesis in Arabidopsis by cytokinin,” The Plant Cell, vol. 24, no. 10. American Society of Plant Biologists, pp. 3967–3981, 2012.","ama":"Bielach A, Podlesakova K, Marhavý P, et al. Spatiotemporal regulation of lateral root organogenesis in Arabidopsis by cytokinin. The Plant Cell. 2012;24(10):3967-3981. doi:10.1105/tpc.112.103044","apa":"Bielach, A., Podlesakova, K., Marhavý, P., Duclercq, J., Cuesta, C., Muller, B., … Benková, E. (2012). Spatiotemporal regulation of lateral root organogenesis in Arabidopsis by cytokinin. The Plant Cell. American Society of Plant Biologists. https://doi.org/10.1105/tpc.112.103044","mla":"Bielach, Agnieszka, et al. “Spatiotemporal Regulation of Lateral Root Organogenesis in Arabidopsis by Cytokinin.” The Plant Cell, vol. 24, no. 10, American Society of Plant Biologists, 2012, pp. 3967–81, doi:10.1105/tpc.112.103044.","ista":"Bielach A, Podlesakova K, Marhavý P, Duclercq J, Cuesta C, Muller B, Grunewald W, Tarkowski P, Benková E. 2012. Spatiotemporal regulation of lateral root organogenesis in Arabidopsis by cytokinin. The Plant Cell. 24(10), 3967–3981.","chicago":"Bielach, Agnieszka, Katerina Podlesakova, Peter Marhavý, Jérôme Duclercq, Candela Cuesta, Bruno Muller, Wim Grunewald, Petr Tarkowski, and Eva Benková. “Spatiotemporal Regulation of Lateral Root Organogenesis in Arabidopsis by Cytokinin.” The Plant Cell. American Society of Plant Biologists, 2012. https://doi.org/10.1105/tpc.112.103044."},"date_updated":"2021-01-12T08:17:55Z","extern":1,"type":"journal_article","status":"public","_id":"829","page":"3967 - 3981","doi":"10.1105/tpc.112.103044","issue":"10","date_published":"2012-10-01T00:00:00Z","volume":24,"date_created":"2018-12-11T11:48:43Z","year":"2012","publication_status":"published","day":"01","publication":"The Plant Cell","publisher":"American Society of Plant Biologists","quality_controlled":0,"month":"10","intvolume":" 24","abstract":[{"text":"The architecture of a plant's root system, established postembryonically, results from both coordinated root growth and lateral root branching. The plant hormones auxin and cytokinin are central endogenous signaling molecules that regulate lateral root organogenesis positively and negatively, respectively. Tight control and mutual balance of their antagonistic activities are particularly important during the early phases of lateral root organogenesis to ensure continuous lateral root initiation (LRI) and proper development of lateral root primordia (LRP). Here, we show that the early phases of lateral root organogenesis, including priming and initiation, take place in root zones with a repressed cytokinin response. Accordingly, ectopic overproduction of cytokinin in the root basal meristem most efficiently inhibits LRI. Enhanced cytokinin responses in pericycle cells between existing LRP might restrict LRI near existing LRP and, when compromised, ectopic LRI occurs. Furthermore, our results demonstrate that young LRP are more sensitive to perturbations in the cytokinin activity than are developmentally more advanced primordia. We hypothesize that the effect of cytokinin on the development of primordia possibly depends on the robustness and stability of the auxin gradient.","lang":"eng"}],"acknowledgement":"We thank Jen Sheen, Dolf Weijers, Tatsuo Kakimoto, Stephen Depuydt, and Laurent Laplaze for sharing published material, Jiri Friml for discussions, and Martine De Cock and Annick Bleys for help in preparing the manuscript. This work was supported by a Starting Independent Research grant from the European Research Council (ERC-2007-Stg-207362-HCPO) and the project CZ.1.07/2.3.00/20.0043 to the Central European Institute of Technology to E.B. and grants from the Ministry of Education, Youth, and Sports of the Czech Republic (MSM 6198959216) and the Centre of the Region Haná for Biotechnological and Agricultural Research (ED0007/01/01) to P.T."},{"abstract":[{"lang":"eng","text":"Cytokinin is an important regulator of plant growth and development. In Arabidopsis thaliana, the two-component phosphorelay mediated through a family of histidine kinases and response regulators is recognized as the principal cytokinin signal transduction mechanism activating the complex transcriptional response to control various developmental processes. Here, we identified an alternative mode of cytokinin action that uses endocytic trafficking as a means to direct plant organogenesis. This activity occurs downstream of known cytokinin receptors but through a branch of the cytokinin signaling pathway that does not involve transcriptional regulation. We show that cytokinin regulates endocytic recycling of the auxin efflux carrier PINFORMED1 (PIN1) by redirecting it for lytic degradation in vacuoles. Stimulation of the lytic PIN1 degradation is not a default effect for general downregulation of proteins from plasma membranes, but a specific mechanism to rapidly modulate the auxin distribution in cytokinin-mediated developmental processes."}],"quality_controlled":0,"publisher":"Cell Press","intvolume":" 21","month":"10","publication_status":"published","year":"2011","publication":"Developmental Cell","day":"18","page":"796 - 804","date_created":"2018-12-11T12:01:22Z","date_published":"2011-10-18T00:00:00Z","doi":"10.1016/j.devcel.2011.08.014","volume":21,"issue":"4","_id":"3097","type":"journal_article","status":"public","date_updated":"2021-01-12T07:41:02Z","citation":{"ista":"Marhavý P, Bielach A, Abas L, Abuzeineh A, Duclercq J, Tanaka H, Pařezová M, Petrášek J, Friml J, Kleine Vehn J, Benková E. 2011. Cytokinin modulates endocytic trafficking of PIN1 auxin efflux carrier to control plant organogenesis. Developmental Cell. 21(4), 796–804.","chicago":"Marhavý, Peter, Agnieszka Bielach, Lindy Abas, Anas Abuzeineh, Jérôme Duclercq, Hirokazu Tanaka, Markéta Pařezová, et al. “Cytokinin Modulates Endocytic Trafficking of PIN1 Auxin Efflux Carrier to Control Plant Organogenesis.” Developmental Cell. Cell Press, 2011. https://doi.org/10.1016/j.devcel.2011.08.014.","apa":"Marhavý, P., Bielach, A., Abas, L., Abuzeineh, A., Duclercq, J., Tanaka, H., … Benková, E. (2011). Cytokinin modulates endocytic trafficking of PIN1 auxin efflux carrier to control plant organogenesis. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2011.08.014","ama":"Marhavý P, Bielach A, Abas L, et al. Cytokinin modulates endocytic trafficking of PIN1 auxin efflux carrier to control plant organogenesis. Developmental Cell. 2011;21(4):796-804. doi:10.1016/j.devcel.2011.08.014","short":"P. Marhavý, A. Bielach, L. Abas, A. Abuzeineh, J. Duclercq, H. Tanaka, M. Pařezová, J. Petrášek, J. Friml, J. Kleine Vehn, E. Benková, Developmental Cell 21 (2011) 796–804.","ieee":"P. Marhavý et al., “Cytokinin modulates endocytic trafficking of PIN1 auxin efflux carrier to control plant organogenesis,” Developmental Cell, vol. 21, no. 4. Cell Press, pp. 796–804, 2011.","mla":"Marhavý, Peter, et al. “Cytokinin Modulates Endocytic Trafficking of PIN1 Auxin Efflux Carrier to Control Plant Organogenesis.” Developmental Cell, vol. 21, no. 4, Cell Press, 2011, pp. 796–804, doi:10.1016/j.devcel.2011.08.014."},"extern":1,"publist_id":"3603","author":[{"first_name":"Peter","id":"3F45B078-F248-11E8-B48F-1D18A9856A87","full_name":"Peter Marhavy","orcid":"0000-0001-5227-5741","last_name":"Marhavy"},{"last_name":"Bielach","full_name":"Bielach, Agnieszka","first_name":"Agnieszka"},{"full_name":"Abas, Lindy","last_name":"Abas","first_name":"Lindy"},{"first_name":"Anas","last_name":"Abuzeineh","full_name":"Abuzeineh, Anas"},{"first_name":"Jérôme","full_name":"Duclercq, Jérôme","last_name":"Duclercq"},{"full_name":"Tanaka, Hirokazu","last_name":"Tanaka","first_name":"Hirokazu"},{"full_name":"Pařezová, Markéta","last_name":"Pařezová","first_name":"Markéta"},{"first_name":"Jan","full_name":"Petrášek, Jan","last_name":"Petrášek"},{"first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Jirí Friml","orcid":"0000-0002-8302-7596"},{"full_name":"Kleine-Vehn, Jürgen","last_name":"Kleine Vehn","first_name":"Jürgen"},{"first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Eva Benková","orcid":"0000-0002-8510-9739","last_name":"Benková"}],"title":"Cytokinin modulates endocytic trafficking of PIN1 auxin efflux carrier to control plant organogenesis"},{"abstract":[{"lang":"eng","text":"The apical hook of dark-grown Arabidopsis seedlings is a simple structure that develops soon after germination to protect the meristem tissues during emergence through the soil and that opens upon exposure to light. Differential growth at the apical hook proceeds in three sequential steps that are regulated by multiple hormones, principally auxin and ethylene. We show that the progress of the apical hook through these developmental phases depends on the dynamic, asymmetric distribution of auxin, which is regulated by auxin efflux carriers of the PIN family. Several PIN proteins exhibited specific, partially overlapping spatial and temporal expression patterns, and their subcellular localization suggested auxin fluxes during hook development. Genetic manipulation of individual PIN activities interfered with different stages of hook development, implying that specific combinations of PIN genes are required for progress of the apical hook through the developmental phases. Furthermore, ethylene might modulate apical hook development by prolonging the formation phase and strongly suppressing the maintenance phase. This ethylene effect is in part mediated by regulation of PIN-dependent auxin efflux and auxin signaling."}],"month":"02","intvolume":" 137","publisher":"Company of Biologists","quality_controlled":0,"day":"15","publication":"Development","year":"2010","publication_status":"published","date_published":"2010-02-15T00:00:00Z","volume":137,"doi":"10.1242/dev.041277","issue":"4","date_created":"2018-12-11T12:01:09Z","page":"607 - 617","_id":"3065","status":"public","type":"journal_article","extern":1,"citation":{"ieee":"P. Žádníková et al., “Role of PIN-mediated auxin efflux in apical hook development of Arabidopsis thaliana,” Development, vol. 137, no. 4. Company of Biologists, pp. 607–617, 2010.","short":"P. Žádníková, J. Petrášek, P. Marhavý, V. Raz, F. Vandenbussche, Z. Ding, K. Schwarzerová, M. Morita, M. Tasaka, J. Hejátko, D. Van Der Straeten, J. Friml, E. Benková, Development 137 (2010) 607–617.","apa":"Žádníková, P., Petrášek, J., Marhavý, P., Raz, V., Vandenbussche, F., Ding, Z., … Benková, E. (2010). Role of PIN-mediated auxin efflux in apical hook development of Arabidopsis thaliana. Development. Company of Biologists. https://doi.org/10.1242/dev.041277","ama":"Žádníková P, Petrášek J, Marhavý P, et al. Role of PIN-mediated auxin efflux in apical hook development of Arabidopsis thaliana. Development. 2010;137(4):607-617. doi:10.1242/dev.041277","mla":"Žádníková, Petra, et al. “Role of PIN-Mediated Auxin Efflux in Apical Hook Development of Arabidopsis Thaliana.” Development, vol. 137, no. 4, Company of Biologists, 2010, pp. 607–17, doi:10.1242/dev.041277.","ista":"Žádníková P, Petrášek J, Marhavý P, Raz V, Vandenbussche F, Ding Z, Schwarzerová K, Morita M, Tasaka M, Hejátko J, Van Der Straeten D, Friml J, Benková E. 2010. Role of PIN-mediated auxin efflux in apical hook development of Arabidopsis thaliana. Development. 137(4), 607–617.","chicago":"Žádníková, Petra, Jan Petrášek, Peter Marhavý, Vered Raz, Filip Vandenbussche, Zhaojun Ding, Kateřina Schwarzerová, et al. “Role of PIN-Mediated Auxin Efflux in Apical Hook Development of Arabidopsis Thaliana.” Development. Company of Biologists, 2010. https://doi.org/10.1242/dev.041277."},"date_updated":"2021-01-12T07:40:48Z","title":"Role of PIN-mediated auxin efflux in apical hook development of Arabidopsis thaliana","author":[{"full_name":"Žádníková, Petra","last_name":"Žádníková","first_name":"Petra"},{"first_name":"Jan","last_name":"Petrášek","full_name":"Petrášek, Jan"},{"first_name":"Peter","id":"3F45B078-F248-11E8-B48F-1D18A9856A87","last_name":"Marhavy","orcid":"0000-0001-5227-5741","full_name":"Peter Marhavy"},{"first_name":"Vered","last_name":"Raz","full_name":"Raz, Vered"},{"full_name":"Vandenbussche, Filip","last_name":"Vandenbussche","first_name":"Filip"},{"full_name":"Ding, Zhaojun","last_name":"Ding","first_name":"Zhaojun"},{"full_name":"Schwarzerová, Kateřina","last_name":"Schwarzerová","first_name":"Kateřina"},{"last_name":"Morita","full_name":"Morita, Miyo T","first_name":"Miyo"},{"first_name":"Masao","last_name":"Tasaka","full_name":"Tasaka, Masao"},{"last_name":"Hejátko","full_name":"Hejátko, Jan","first_name":"Jan"},{"first_name":"Dominique","last_name":"Van Der Straeten","full_name":"Van Der Straeten, Dominique"},{"last_name":"Friml","full_name":"Jirí Friml","orcid":"0000-0002-8302-7596","first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Benková","full_name":"Eva Benková","orcid":"0000-0002-8510-9739","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"}],"publist_id":"3636"}]