[{"_id":"7427","article_type":"original","type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"status":"public","date_updated":"2024-03-27T23:30:37Z","ddc":["580"],"department":[{"_id":"JiFr"},{"_id":"EvBe"}],"file_date_updated":"2020-09-22T09:51:28Z","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"abstract":[{"lang":"eng","text":"Plants, like other multicellular organisms, survive through a delicate balance between growth and defense against pathogens. Salicylic acid (SA) is a major defense signal in plants, and the perception mechanism as well as downstream signaling activating the immune response are known. Here, we identify a parallel SA signaling that mediates growth attenuation. SA directly binds to A subunits of protein phosphatase 2A (PP2A), inhibiting activity of this complex. Among PP2A targets, the PIN2 auxin transporter is hyperphosphorylated in response to SA, leading to changed activity of this important growth regulator. Accordingly, auxin transport and auxin-mediated root development, including growth, gravitropic response, and lateral root organogenesis, are inhibited. This study reveals how SA, besides activating immunity, concomitantly attenuates growth through crosstalk with the auxin distribution network. Further analysis of this dual role of SA and characterization of additional SA-regulated PP2A targets will provide further insights into mechanisms maintaining a balance between growth and defense."}],"pmid":1,"oa_version":"Published Version","scopus_import":"1","month":"02","intvolume":" 30","publication_identifier":{"issn":["09609822"]},"publication_status":"published","file":[{"access_level":"open_access","relation":"main_file","content_type":"application/pdf","file_id":"8555","checksum":"16f7d51fe28f91c21e4896a2028df40b","success":1,"creator":"dernst","date_updated":"2020-09-22T09:51:28Z","file_size":5360135,"date_created":"2020-09-22T09:51:28Z","file_name":"2020_CurrentBiology_Tan.pdf"}],"language":[{"iso":"eng"}],"volume":30,"issue":"3","related_material":{"record":[{"relation":"dissertation_contains","id":"8822","status":"public"}]},"ec_funded":1,"license":"https://creativecommons.org/licenses/by/4.0/","project":[{"_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","grant_number":"742985"},{"call_identifier":"FP7","_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme","grant_number":"291734"},{"_id":"256FEF10-B435-11E9-9278-68D0E5697425","name":"Long Term Fellowship","grant_number":"723-2015"}],"citation":{"apa":"Tan, S., Abas, M. F., Verstraeten, I., Glanc, M., Molnar, G., Hajny, J., … Friml, J. (2020). Salicylic acid targets protein phosphatase 2A to attenuate growth in plants. Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2019.11.058","ama":"Tan S, Abas MF, Verstraeten I, et al. Salicylic acid targets protein phosphatase 2A to attenuate growth in plants. Current Biology. 2020;30(3):381-395.e8. doi:10.1016/j.cub.2019.11.058","short":"S. Tan, M.F. Abas, I. Verstraeten, M. Glanc, G. Molnar, J. Hajny, P. Lasák, I. Petřík, E. Russinova, J. Petrášek, O. Novák, J. Pospíšil, J. Friml, Current Biology 30 (2020) 381–395.e8.","ieee":"S. Tan et al., “Salicylic acid targets protein phosphatase 2A to attenuate growth in plants,” Current Biology, vol. 30, no. 3. Cell Press, p. 381–395.e8, 2020.","mla":"Tan, Shutang, et al. “Salicylic Acid Targets Protein Phosphatase 2A to Attenuate Growth in Plants.” Current Biology, vol. 30, no. 3, Cell Press, 2020, p. 381–395.e8, doi:10.1016/j.cub.2019.11.058.","ista":"Tan S, Abas MF, Verstraeten I, Glanc M, Molnar G, Hajny J, Lasák P, Petřík I, Russinova E, Petrášek J, Novák O, Pospíšil J, Friml J. 2020. Salicylic acid targets protein phosphatase 2A to attenuate growth in plants. Current Biology. 30(3), 381–395.e8.","chicago":"Tan, Shutang, Melinda F Abas, Inge Verstraeten, Matous Glanc, Gergely Molnar, Jakub Hajny, Pavel Lasák, et al. “Salicylic Acid Targets Protein Phosphatase 2A to Attenuate Growth in Plants.” Current Biology. Cell Press, 2020. https://doi.org/10.1016/j.cub.2019.11.058."},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","author":[{"first_name":"Shutang","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","last_name":"Tan","orcid":"0000-0002-0471-8285","full_name":"Tan, Shutang"},{"id":"3CFB3B1C-F248-11E8-B48F-1D18A9856A87","first_name":"Melinda F","full_name":"Abas, Melinda F","last_name":"Abas"},{"id":"362BF7FE-F248-11E8-B48F-1D18A9856A87","first_name":"Inge","last_name":"Verstraeten","orcid":"0000-0001-7241-2328","full_name":"Verstraeten, Inge"},{"full_name":"Glanc, Matous","orcid":"0000-0003-0619-7783","last_name":"Glanc","id":"1AE1EA24-02D0-11E9-9BAA-DAF4881429F2","first_name":"Matous"},{"first_name":"Gergely","id":"34F1AF46-F248-11E8-B48F-1D18A9856A87","full_name":"Molnar, Gergely","last_name":"Molnar"},{"last_name":"Hajny","orcid":"0000-0003-2140-7195","full_name":"Hajny, Jakub","id":"4800CC20-F248-11E8-B48F-1D18A9856A87","first_name":"Jakub"},{"first_name":"Pavel","full_name":"Lasák, Pavel","last_name":"Lasák"},{"last_name":"Petřík","full_name":"Petřík, Ivan","first_name":"Ivan"},{"first_name":"Eugenia","full_name":"Russinova, Eugenia","last_name":"Russinova"},{"last_name":"Petrášek","full_name":"Petrášek, Jan","first_name":"Jan"},{"first_name":"Ondřej","full_name":"Novák, Ondřej","last_name":"Novák"},{"full_name":"Pospíšil, Jiří","last_name":"Pospíšil","first_name":"Jiří"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří"}],"external_id":{"pmid":["31956021"],"isi":["000511287900018"]},"article_processing_charge":"No","title":"Salicylic acid targets protein phosphatase 2A to attenuate growth in plants","acknowledgement":"We thank Shigeyuki Betsuyaku (University of Tsukuba), Alison Delong (Brown University), Xinnian Dong (Duke University), Dolf Weijers (Wageningen University), Yuelin Zhang (UBC), and Martine Pastuglia (Institut Jean-Pierre Bourgin) for sharing published materials; Jana Riederer for help with cantharidin physiological analysis; David Domjan for help with cloning pET28a-PIN2HL; Qing Lu for help with DARTS; Hana Kozubı´kova´ for technical support on SA derivative synthesis; Zuzana Vondra´ kova´ for technical support with tobacco cells; Lucia Strader (Washington University), Bert De Rybel (Ghent University), Bartel Vanholme (Ghent University), and Lukas Mach (BOKU) for helpful discussions; and bioimaging and life science facilities of IST Austria for continuous support. We gratefully acknowledge the Nottingham Arabidopsis Stock Center (NASC) for providing T-DNA insertional mutants. The DSC and SPR instruments were provided by the EQ-BOKU VIBT GmbH and the BOKU Core Facility for Biomolecular and Cellular Analysis, with help of Irene Schaffner. The research leading to these results has received funding from the European Union’s Horizon 2020 program (ERC grant agreement no. 742985 to J.F.) and the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013) under REA grant agreement no. 291734. S.T. was supported by a European Molecular Biology Organization (EMBO) long-term postdoctoral fellowship (ALTF 723-2015). O.N. was supported by the Ministry of Education, Youth and Sports of the Czech Republic (European Regional Development Fund-Project ‘‘Centre for Experimental Plant Biology’’ no. CZ.02.1.01/0.0/0.0/16_019/0000738). J. Pospısil was supported by European Regional Development Fund Project ‘‘Centre for Experimental Plant Biology’’\r\n(no. CZ.02.1.01/0.0/0.0/16_019/0000738). J. Petrasek was supported by EU Operational Programme Prague-Competitiveness (no. CZ.2.16/3.1.00/21519). ","publisher":"Cell Press","quality_controlled":"1","oa":1,"isi":1,"has_accepted_license":"1","year":"2020","day":"03","publication":"Current Biology","page":"381-395.e8","doi":"10.1016/j.cub.2019.11.058","date_published":"2020-02-03T00:00:00Z","date_created":"2020-02-02T23:01:00Z"},{"_id":"191","status":"public","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"type":"journal_article","ddc":["581"],"date_updated":"2024-03-27T23:30:37Z","department":[{"_id":"JiFr"},{"_id":"EvBe"}],"file_date_updated":"2020-07-14T12:45:20Z","oa_version":"Published Version","abstract":[{"lang":"eng","text":"Intercellular distribution of the plant hormone auxin largely depends on the polar subcellular distribution of the plasma membrane PIN-FORMED (PIN) auxin transporters. PIN polarity switches in response to different developmental and environmental signals have been shown to redirect auxin fluxes mediating certain developmental responses. PIN phosphorylation at different sites and by different kinases is crucial for PIN function. Here we investigate the role of PIN phosphorylation during gravitropic response. Loss- and gain-of-function mutants in PINOID and related kinases but not in D6PK kinase as well as mutations mimicking constitutive dephosphorylated or phosphorylated status of two clusters of predicted phosphorylation sites partially disrupted PIN3 phosphorylation and caused defects in gravitropic bending in roots and hypocotyls. In particular, they impacted PIN3 polarity rearrangements in response to gravity and during feed-back regulation by auxin itself. Thus PIN phosphorylation, besides regulating transport activity and apical-basal targeting, is also important for the rapid polarity switches in response to environmental and endogenous signals."}],"intvolume":" 8","month":"07","scopus_import":"1","language":[{"iso":"eng"}],"file":[{"checksum":"266b03f4fb8198e83141617aaa99dcab","file_id":"5714","content_type":"application/pdf","relation":"main_file","access_level":"open_access","file_name":"2018_ScientificReports_Grones.pdf","date_created":"2018-12-17T15:38:56Z","file_size":2413876,"date_updated":"2020-07-14T12:45:20Z","creator":"dernst"}],"publication_status":"published","ec_funded":1,"issue":"1","volume":8,"related_material":{"record":[{"relation":"dissertation_contains","id":"8822","status":"public"}]},"article_number":"10279","project":[{"name":"Polarity and subcellular dynamics in plants","grant_number":"282300","call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425"},{"grant_number":"742985","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020","_id":"261099A6-B435-11E9-9278-68D0E5697425"}],"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","citation":{"ista":"Grones P, Abas MF, Hajny J, Jones A, Waidmann S, Kleine Vehn J, Friml J. 2018. PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism. Scientific Reports. 8(1), 10279.","chicago":"Grones, Peter, Melinda F Abas, Jakub Hajny, Angharad Jones, Sascha Waidmann, Jürgen Kleine Vehn, and Jiří Friml. “PID/WAG-Mediated Phosphorylation of the Arabidopsis PIN3 Auxin Transporter Mediates Polarity Switches during Gravitropism.” Scientific Reports. Springer, 2018. https://doi.org/10.1038/s41598-018-28188-1.","short":"P. Grones, M.F. Abas, J. Hajny, A. Jones, S. Waidmann, J. Kleine Vehn, J. Friml, Scientific Reports 8 (2018).","ieee":"P. Grones et al., “PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism,” Scientific Reports, vol. 8, no. 1. Springer, 2018.","apa":"Grones, P., Abas, M. F., Hajny, J., Jones, A., Waidmann, S., Kleine Vehn, J., & Friml, J. (2018). PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism. Scientific Reports. Springer. https://doi.org/10.1038/s41598-018-28188-1","ama":"Grones P, Abas MF, Hajny J, et al. PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism. Scientific Reports. 2018;8(1). doi:10.1038/s41598-018-28188-1","mla":"Grones, Peter, et al. “PID/WAG-Mediated Phosphorylation of the Arabidopsis PIN3 Auxin Transporter Mediates Polarity Switches during Gravitropism.” Scientific Reports, vol. 8, no. 1, 10279, Springer, 2018, doi:10.1038/s41598-018-28188-1."},"title":"PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism","article_processing_charge":"No","external_id":{"isi":["000437673200053"]},"author":[{"full_name":"Grones, Peter","last_name":"Grones","id":"399876EC-F248-11E8-B48F-1D18A9856A87","first_name":"Peter"},{"full_name":"Abas, Melinda F","last_name":"Abas","first_name":"Melinda F","id":"3CFB3B1C-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Jakub","id":"4800CC20-F248-11E8-B48F-1D18A9856A87","last_name":"Hajny","orcid":"0000-0003-2140-7195","full_name":"Hajny, Jakub"},{"full_name":"Jones, Angharad","last_name":"Jones","first_name":"Angharad"},{"first_name":"Sascha","full_name":"Waidmann, Sascha","last_name":"Waidmann"},{"first_name":"Jürgen","last_name":"Kleine Vehn","full_name":"Kleine Vehn, Jürgen"},{"last_name":"Friml","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"publist_id":"7729","oa":1,"quality_controlled":"1","publisher":"Springer","publication":"Scientific Reports","day":"06","year":"2018","isi":1,"has_accepted_license":"1","date_created":"2018-12-11T11:45:06Z","date_published":"2018-07-06T00:00:00Z","doi":"10.1038/s41598-018-28188-1"},{"year":"2006","publication_status":"published","day":"12","publication":"Science","page":"914 - 918","issue":"5775","doi":"10.1126/science.1123542","volume":312,"date_published":"2006-05-12T00:00:00Z","date_created":"2018-12-11T12:00:51Z","abstract":[{"text":"Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport-related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux.","lang":"eng"}],"quality_controlled":0,"publisher":"American Association for the Advancement of Science","month":"05","intvolume":" 312","date_updated":"2021-01-12T07:40:27Z","citation":{"chicago":"Petrášek, Jan, Jozef Mravec, Rodolphe Bouchard, Joshua Blakeslee, Melinda F Abas, Daniela Seifertová, Justyna Wiśniewska, et al. “PIN Proteins Perform a Rate-Limiting Function in Cellular Auxin Efflux.” Science. American Association for the Advancement of Science, 2006. https://doi.org/10.1126/science.1123542.","ista":"Petrášek J, Mravec J, Bouchard R, Blakeslee J, Abas MF, Seifertová D, Wiśniewska J, Tadele Z, Kubeš M, Čovanová M, Dhonukshe P, Skůpa P, Benková E, Perry L, Křeček P, Lee O, Fink G, Geisler M, Murphy A, Luschnig C, Zažímalová E, Friml J. 2006. PIN proteins perform a rate-limiting function in cellular auxin efflux. Science. 312(5775), 914–918.","mla":"Petrášek, Jan, et al. “PIN Proteins Perform a Rate-Limiting Function in Cellular Auxin Efflux.” Science, vol. 312, no. 5775, American Association for the Advancement of Science, 2006, pp. 914–18, doi:10.1126/science.1123542.","ieee":"J. Petrášek et al., “PIN proteins perform a rate-limiting function in cellular auxin efflux,” Science, vol. 312, no. 5775. American Association for the Advancement of Science, pp. 914–918, 2006.","short":"J. Petrášek, J. Mravec, R. Bouchard, J. Blakeslee, M.F. Abas, D. Seifertová, J. Wiśniewska, Z. Tadele, M. Kubeš, M. Čovanová, P. Dhonukshe, P. Skůpa, E. Benková, L. Perry, P. Křeček, O. Lee, G. Fink, M. Geisler, A. Murphy, C. Luschnig, E. Zažímalová, J. Friml, Science 312 (2006) 914–918.","apa":"Petrášek, J., Mravec, J., Bouchard, R., Blakeslee, J., Abas, M. F., Seifertová, D., … Friml, J. (2006). PIN proteins perform a rate-limiting function in cellular auxin efflux. Science. American Association for the Advancement of Science. https://doi.org/10.1126/science.1123542","ama":"Petrášek J, Mravec J, Bouchard R, et al. PIN proteins perform a rate-limiting function in cellular auxin efflux. Science. 2006;312(5775):914-918. doi:10.1126/science.1123542"},"extern":1,"publist_id":"3690","author":[{"first_name":"Jan","full_name":"Petrášek, Jan","last_name":"Petrášek"},{"first_name":"Jozef","last_name":"Mravec","full_name":"Mravec, Jozef"},{"first_name":"Rodolphe","last_name":"Bouchard","full_name":"Bouchard, Rodolphe"},{"first_name":"Joshua","full_name":"Blakeslee, Joshua","last_name":"Blakeslee"},{"last_name":"Abas","full_name":"Melinda Abas","id":"3CFB3B1C-F248-11E8-B48F-1D18A9856A87","first_name":"Melinda F"},{"first_name":"Daniela","last_name":"Seifertová","full_name":"Seifertová, Daniela"},{"first_name":"Justyna","last_name":"Wiśniewska","full_name":"Wiśniewska, Justyna"},{"last_name":"Tadele","full_name":"Tadele, Zerihun","first_name":"Zerihun"},{"first_name":"Martin","last_name":"Kubeš","full_name":"Kubeš, Martin"},{"last_name":"Čovanová","full_name":"Čovanová, Milada","first_name":"Milada"},{"first_name":"Pankaj","last_name":"Dhonukshe","full_name":"Dhonukshe, Pankaj"},{"last_name":"Skůpa","full_name":"Skůpa, Petr","first_name":"Petr"},{"last_name":"Benková","full_name":"Eva Benková","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva"},{"last_name":"Perry","full_name":"Perry, Lucie","first_name":"Lucie"},{"first_name":"Pavel","full_name":"Křeček, Pavel","last_name":"Křeček"},{"last_name":"Lee","full_name":"Lee, Ok Ran","first_name":"Ok"},{"first_name":"Gerald","last_name":"Fink","full_name":"Fink, Gerald R"},{"first_name":"Markus","full_name":"Geisler, Markus","last_name":"Geisler"},{"first_name":"Angus","full_name":"Murphy, Angus S","last_name":"Murphy"},{"first_name":"Christian","full_name":"Luschnig, Christian","last_name":"Luschnig"},{"full_name":"Zažímalová, Eva","last_name":"Zažímalová","first_name":"Eva"},{"orcid":"0000-0002-8302-7596","full_name":"Jirí Friml","last_name":"Friml","first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"title":"PIN proteins perform a rate-limiting function in cellular auxin efflux","_id":"3012","type":"journal_article","status":"public"}]