[{"type":"journal_article","article_type":"original","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"status":"public","_id":"14795","department":[{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"MaLo"},{"_id":"NanoFab"}],"file_date_updated":"2024-01-16T10:53:31Z","date_updated":"2024-01-17T08:20:40Z","ddc":["570"],"scopus_import":"1","month":"01","intvolume":" 34","abstract":[{"text":"Metazoan development relies on the formation and remodeling of cell-cell contacts. Dynamic reorganization of adhesion receptors and the actomyosin cell cortex in space and time plays a central role in cell-cell contact formation and maturation. Nevertheless, how this process is mechanistically achieved when new contacts are formed remains unclear. Here, by building a biomimetic assay composed of progenitor cells adhering to supported lipid bilayers functionalized with E-cadherin ectodomains, we show that cortical F-actin flows, driven by the depletion of myosin-2 at the cell contact center, mediate the dynamic reorganization of adhesion receptors and cell cortex at the contact. E-cadherin-dependent downregulation of the small GTPase RhoA at the forming contact leads to both a depletion of myosin-2 and a decrease of F-actin at the contact center. At the contact rim, in contrast, myosin-2 becomes enriched by the retraction of bleb-like protrusions, resulting in a cortical tension gradient from the contact rim to its center. This tension gradient, in turn, triggers centrifugal F-actin flows, leading to further accumulation of F-actin at the contact rim and the progressive redistribution of E-cadherin from the contact center to the rim. Eventually, this combination of actomyosin downregulation and flows at the contact determines the characteristic molecular organization, with E-cadherin and F-actin accumulating at the contact rim, where they are needed to mechanically link the contractile cortices of the adhering cells.","lang":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"}],"oa_version":"Published Version","issue":"1","volume":34,"ec_funded":1,"publication_identifier":{"eissn":["1879-0445"],"issn":["0960-9822"]},"publication_status":"published","file":[{"success":1,"checksum":"51220b76d72a614208f84bdbfbaf9b72","file_id":"14813","content_type":"application/pdf","relation":"main_file","access_level":"open_access","file_name":"2024_CurrentBiology_Arslan.pdf","date_created":"2024-01-16T10:53:31Z","file_size":5183861,"date_updated":"2024-01-16T10:53:31Z","creator":"dernst"}],"language":[{"iso":"eng"}],"project":[{"_id":"260F1432-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"742573","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation"}],"author":[{"last_name":"Arslan","orcid":"0000-0001-5809-9566","full_name":"Arslan, Feyza N","id":"49DA7910-F248-11E8-B48F-1D18A9856A87","first_name":"Feyza N"},{"last_name":"Hannezo","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"},{"first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin","full_name":"Merrin, Jack","orcid":"0000-0001-5145-4609"},{"last_name":"Loose","orcid":"0000-0001-7309-9724","full_name":"Loose, Martin","id":"462D4284-F248-11E8-B48F-1D18A9856A87","first_name":"Martin"},{"last_name":"Heisenberg","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"article_processing_charge":"Yes (via OA deal)","title":"Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts","citation":{"chicago":"Arslan, Feyza N, Edouard B Hannezo, Jack Merrin, Martin Loose, and Carl-Philipp J Heisenberg. “Adhesion-Induced Cortical Flows Pattern E-Cadherin-Mediated Cell Contacts.” Current Biology. Elsevier, 2024. https://doi.org/10.1016/j.cub.2023.11.067.","ista":"Arslan FN, Hannezo EB, Merrin J, Loose M, Heisenberg C-PJ. 2024. Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts. Current Biology. 34(1), 171–182.e8.","mla":"Arslan, Feyza N., et al. “Adhesion-Induced Cortical Flows Pattern E-Cadherin-Mediated Cell Contacts.” Current Biology, vol. 34, no. 1, Elsevier, 2024, p. 171–182.e8, doi:10.1016/j.cub.2023.11.067.","ieee":"F. N. Arslan, E. B. Hannezo, J. Merrin, M. Loose, and C.-P. J. Heisenberg, “Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts,” Current Biology, vol. 34, no. 1. Elsevier, p. 171–182.e8, 2024.","short":"F.N. Arslan, E.B. Hannezo, J. Merrin, M. Loose, C.-P.J. Heisenberg, Current Biology 34 (2024) 171–182.e8.","apa":"Arslan, F. N., Hannezo, E. B., Merrin, J., Loose, M., & Heisenberg, C.-P. J. (2024). Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts. Current Biology. Elsevier. https://doi.org/10.1016/j.cub.2023.11.067","ama":"Arslan FN, Hannezo EB, Merrin J, Loose M, Heisenberg C-PJ. Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts. Current Biology. 2024;34(1):171-182.e8. doi:10.1016/j.cub.2023.11.067"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","quality_controlled":"1","publisher":"Elsevier","oa":1,"acknowledgement":"We are grateful to Edwin Munro for their feedback and help with the single particle analysis. We thank members of the Heisenberg and Loose labs for their help and feedback on the manuscript, notably Xin Tong for making the PCS2-mCherry-AHPH plasmid. Finally, we thank the Aquatics and Imaging & Optics facilities of ISTA for their continuous support, especially Yann Cesbron for assistance with the laser cutter. This work was supported by an ERC\r\nAdvanced Grant (MECSPEC) to C.-P.H.","page":"171-182.e8","doi":"10.1016/j.cub.2023.11.067","date_published":"2024-01-08T00:00:00Z","date_created":"2024-01-14T23:00:56Z","has_accepted_license":"1","year":"2024","day":"08","publication":"Current Biology"},{"author":[{"first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","last_name":"Hannezo"},{"last_name":"Scheele","full_name":"Scheele, Colinda L.G.J.","first_name":"Colinda L.G.J."}],"external_id":{"pmid":["36653709"]},"article_processing_charge":"No","title":"A Guide Toward Multi-scale and Quantitative Branching Analysis in the Mammary Gland","editor":[{"full_name":"Margadant, Coert","last_name":"Margadant","first_name":"Coert"}],"citation":{"mla":"Hannezo, Edouard B., and Colinda L. G. J. Scheele. “A Guide Toward Multi-Scale and Quantitative Branching Analysis in the Mammary Gland.” Cell Migration in Three Dimensions, edited by Coert Margadant, vol. 2608, Springer Nature, 2023, pp. 183–205, doi:10.1007/978-1-0716-2887-4_12.","ama":"Hannezo EB, Scheele CLGJ. A Guide Toward Multi-scale and Quantitative Branching Analysis in the Mammary Gland. In: Margadant C, ed. Cell Migration in Three Dimensions. Vol 2608. MIMB. Springer Nature; 2023:183-205. doi:10.1007/978-1-0716-2887-4_12","apa":"Hannezo, E. B., & Scheele, C. L. G. J. (2023). A Guide Toward Multi-scale and Quantitative Branching Analysis in the Mammary Gland. In C. Margadant (Ed.), Cell Migration in Three Dimensions (Vol. 2608, pp. 183–205). Springer Nature. https://doi.org/10.1007/978-1-0716-2887-4_12","ieee":"E. B. Hannezo and C. L. G. J. Scheele, “A Guide Toward Multi-scale and Quantitative Branching Analysis in the Mammary Gland,” in Cell Migration in Three Dimensions, vol. 2608, C. Margadant, Ed. Springer Nature, 2023, pp. 183–205.","short":"E.B. Hannezo, C.L.G.J. Scheele, in:, C. Margadant (Ed.), Cell Migration in Three Dimensions, Springer Nature, 2023, pp. 183–205.","chicago":"Hannezo, Edouard B, and Colinda L.G.J. Scheele. “A Guide Toward Multi-Scale and Quantitative Branching Analysis in the Mammary Gland.” In Cell Migration in Three Dimensions, edited by Coert Margadant, 2608:183–205. MIMB. Springer Nature, 2023. https://doi.org/10.1007/978-1-0716-2887-4_12.","ista":"Hannezo EB, Scheele CLGJ. 2023.A Guide Toward Multi-scale and Quantitative Branching Analysis in the Mammary Gland. In: Cell Migration in Three Dimensions. Methods in Molecular Biology, vol. 2608, 183–205."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","page":"183-205","doi":"10.1007/978-1-0716-2887-4_12","date_published":"2023-01-19T00:00:00Z","date_created":"2023-01-29T23:00:58Z","has_accepted_license":"1","year":"2023","day":"19","publication":"Cell Migration in Three Dimensions","publisher":"Springer Nature","quality_controlled":"1","oa":1,"department":[{"_id":"EdHa"}],"file_date_updated":"2023-02-03T10:56:39Z","date_updated":"2023-02-03T10:58:56Z","ddc":["570"],"type":"book_chapter","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"status":"public","series_title":"MIMB","_id":"12428","volume":2608,"publication_identifier":{"eisbn":["9781071628874"],"isbn":["9781071628867"],"eissn":["1940-6029"]},"publication_status":"published","file":[{"checksum":"aec1b8d3ba938ddf9d8fcb777f3c38ee","file_id":"12500","success":1,"content_type":"application/pdf","access_level":"open_access","relation":"main_file","date_created":"2023-02-03T10:56:39Z","file_name":"2023_MIMB_Hannezo.pdf","date_updated":"2023-02-03T10:56:39Z","file_size":826598,"creator":"dernst"}],"language":[{"iso":"eng"}],"scopus_import":"1","alternative_title":["Methods in Molecular Biology"],"month":"01","intvolume":" 2608","abstract":[{"text":"The mammary gland consists of a bilayered epithelial structure with an extensively branched morphology. The majority of this epithelial tree is laid down during puberty, during which actively proliferating terminal end buds repeatedly elongate and bifurcate to form the basic structure of the ductal tree. Mammary ducts consist of a basal and luminal cell layer with a multitude of identified sub-lineages within both layers. The understanding of how these different cell lineages are cooperatively driving branching morphogenesis is a problem of crossing multiple scales, as this requires information on the macroscopic branched structure of the gland, as well as data on single-cell dynamics driving the morphogenic program. Here we describe a method to combine genetic lineage tracing with whole-gland branching analysis. Quantitative data on the global organ structure can be used to derive a model for mammary gland branching morphogenesis and provide a backbone on which the dynamics of individual cell lineages can be simulated and compared to lineage-tracing approaches. Eventually, these quantitative models and experiments allow to understand the couplings between the macroscopic shape of the mammary gland and the underlying single-cell dynamics driving branching morphogenesis.","lang":"eng"}],"pmid":1,"oa_version":"Published Version"},{"article_number":"013001","project":[{"grant_number":"851288","name":"Design Principles of Branching Morphogenesis","call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"mla":"Boocock, Daniel R., et al. “Interplay between Mechanochemical Patterning and Glassy Dynamics in Cellular Monolayers.” PRX Life, vol. 1, no. 1, 013001, American Physical Society, 2023, doi:10.1103/prxlife.1.013001.","ieee":"D. R. Boocock, T. Hirashima, and E. B. Hannezo, “Interplay between mechanochemical patterning and glassy dynamics in cellular monolayers,” PRX Life, vol. 1, no. 1. American Physical Society, 2023.","short":"D.R. Boocock, T. Hirashima, E.B. Hannezo, PRX Life 1 (2023).","ama":"Boocock DR, Hirashima T, Hannezo EB. Interplay between mechanochemical patterning and glassy dynamics in cellular monolayers. PRX Life. 2023;1(1). doi:10.1103/prxlife.1.013001","apa":"Boocock, D. R., Hirashima, T., & Hannezo, E. B. (2023). Interplay between mechanochemical patterning and glassy dynamics in cellular monolayers. PRX Life. American Physical Society. https://doi.org/10.1103/prxlife.1.013001","chicago":"Boocock, Daniel R, Tsuyoshi Hirashima, and Edouard B Hannezo. “Interplay between Mechanochemical Patterning and Glassy Dynamics in Cellular Monolayers.” PRX Life. American Physical Society, 2023. https://doi.org/10.1103/prxlife.1.013001.","ista":"Boocock DR, Hirashima T, Hannezo EB. 2023. Interplay between mechanochemical patterning and glassy dynamics in cellular monolayers. PRX Life. 1(1), 013001."},"title":"Interplay between mechanochemical patterning and glassy dynamics in cellular monolayers","author":[{"full_name":"Boocock, Daniel R","orcid":"0000-0002-1585-2631","last_name":"Boocock","id":"453AF628-F248-11E8-B48F-1D18A9856A87","first_name":"Daniel R"},{"first_name":"Tsuyoshi","full_name":"Hirashima, Tsuyoshi","last_name":"Hirashima"},{"last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"}],"article_processing_charge":"Yes","acknowledgement":"We thank all members of the Hannezo group for discussions and suggestions, as well as Sound Wai Phow for technical assistance. This work received funding from the European Research Council under the EU Horizon 2020 research and innovation program Grant Agreement No. 851288 (E.H.), JSPS KAKENHI Grant No. 21H05290, and the Ministry of Education under the Research Centres of Excellence program through the MBI at NUS.","quality_controlled":"1","publisher":"American Physical Society","oa":1,"day":"20","publication":"PRX Life","has_accepted_license":"1","year":"2023","doi":"10.1103/prxlife.1.013001","date_published":"2023-07-20T00:00:00Z","date_created":"2023-09-06T08:30:59Z","_id":"14277","status":"public","type":"journal_article","article_type":"original","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"ddc":["570"],"date_updated":"2023-09-15T06:39:17Z","file_date_updated":"2023-09-15T06:30:50Z","department":[{"_id":"EdHa"}],"oa_version":"Published Version","abstract":[{"text":"Living tissues are characterized by an intrinsically mechanochemical interplay of active physical forces and complex biochemical signaling pathways. Either feature alone can give rise to complex emergent phenomena, for example, mechanically driven glassy dynamics and rigidity transitions, or chemically driven reaction-diffusion instabilities. An important question is how to quantitatively assess the contribution of these different cues to the large-scale dynamics of biological materials. We address this in Madin-Darby canine kidney (MDCK) monolayers, considering both mechanochemical feedback between extracellular signal-regulated kinase (ERK) signaling activity and cellular density as well as a mechanically active tissue rheology via a self-propelled vertex model. We show that the relative strength of active migration forces to mechanochemical couplings controls a transition from a uniform active glass to periodic spatiotemporal waves. We parametrize the model from published experimental data sets on MDCK monolayers and use it to make new predictions on the correlation functions of cellular dynamics and the dynamics of topological defects associated with the oscillatory phase of cells. Interestingly, MDCK monolayers are best described by an intermediary parameter region in which both mechanochemical couplings and noisy active propulsion have a strong influence on the dynamics. Finally, we study how tissue rheology and ERK waves produce feedback on one another and uncover a mechanism via which tissue fluidity can be controlled by mechanochemical waves at both the local and global levels.","lang":"eng"}],"month":"07","intvolume":" 1","file":[{"creator":"dernst","file_size":2559520,"date_updated":"2023-09-15T06:30:50Z","file_name":"2023_PRXLife_Boocock.pdf","date_created":"2023-09-15T06:30:50Z","relation":"main_file","access_level":"open_access","content_type":"application/pdf","success":1,"file_id":"14335","checksum":"f881d98c89eb9f1aa136d7b781511553"}],"language":[{"iso":"eng"}],"publication_identifier":{"issn":["2835-8279"]},"publication_status":"published","issue":"1","volume":1,"ec_funded":1},{"article_number":"2206110","citation":{"ama":"Schamberger B, Ziege R, Anselme K, et al. Curvature in biological systems: Its quantification, emergence, and implications across the scales. Advanced Materials. 2023;35(13). doi:10.1002/adma.202206110","apa":"Schamberger, B., Ziege, R., Anselme, K., Ben Amar, M., Bykowski, M., Castro, A. P. G., … Dunlop, J. W. C. (2023). Curvature in biological systems: Its quantification, emergence, and implications across the scales. Advanced Materials. Wiley. https://doi.org/10.1002/adma.202206110","short":"B. Schamberger, R. Ziege, K. Anselme, M. Ben Amar, M. Bykowski, A.P.G. Castro, A. Cipitria, R.A. Coles, R. Dimova, M. Eder, S. Ehrig, L.M. Escudero, M.E. Evans, P.R. Fernandes, P. Fratzl, L. Geris, N. Gierlinger, E.B. Hannezo, A. Iglič, J.J.K. Kirkensgaard, P. Kollmannsberger, Ł. Kowalewska, N.A. Kurniawan, I. Papantoniou, L. Pieuchot, T.H.V. Pires, L.D. Renner, A.O. Sageman-Furnas, G.E. Schröder-Turk, A. Sengupta, V.R. Sharma, A. Tagua, C. Tomba, X. Trepat, S.L. Waters, E.F. Yeo, A. Roschger, C.M. Bidan, J.W.C. Dunlop, Advanced Materials 35 (2023).","ieee":"B. Schamberger et al., “Curvature in biological systems: Its quantification, emergence, and implications across the scales,” Advanced Materials, vol. 35, no. 13. Wiley, 2023.","mla":"Schamberger, Barbara, et al. “Curvature in Biological Systems: Its Quantification, Emergence, and Implications across the Scales.” Advanced Materials, vol. 35, no. 13, 2206110, Wiley, 2023, doi:10.1002/adma.202206110.","ista":"Schamberger B, Ziege R, Anselme K, Ben Amar M, Bykowski M, Castro APG, Cipitria A, Coles RA, Dimova R, Eder M, Ehrig S, Escudero LM, Evans ME, Fernandes PR, Fratzl P, Geris L, Gierlinger N, Hannezo EB, Iglič A, Kirkensgaard JJK, Kollmannsberger P, Kowalewska Ł, Kurniawan NA, Papantoniou I, Pieuchot L, Pires THV, Renner LD, Sageman-Furnas AO, Schröder-Turk GE, Sengupta A, Sharma VR, Tagua A, Tomba C, Trepat X, Waters SL, Yeo EF, Roschger A, Bidan CM, Dunlop JWC. 2023. Curvature in biological systems: Its quantification, emergence, and implications across the scales. Advanced Materials. 35(13), 2206110.","chicago":"Schamberger, Barbara, Ricardo Ziege, Karine Anselme, Martine Ben Amar, Michał Bykowski, André P.G. Castro, Amaia Cipitria, et al. “Curvature in Biological Systems: Its Quantification, Emergence, and Implications across the Scales.” Advanced Materials. Wiley, 2023. https://doi.org/10.1002/adma.202206110."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"first_name":"Barbara","last_name":"Schamberger","full_name":"Schamberger, Barbara"},{"first_name":"Ricardo","full_name":"Ziege, Ricardo","last_name":"Ziege"},{"full_name":"Anselme, Karine","last_name":"Anselme","first_name":"Karine"},{"full_name":"Ben Amar, Martine","last_name":"Ben Amar","first_name":"Martine"},{"first_name":"Michał","last_name":"Bykowski","full_name":"Bykowski, Michał"},{"first_name":"André P.G.","last_name":"Castro","full_name":"Castro, André P.G."},{"first_name":"Amaia","full_name":"Cipitria, Amaia","last_name":"Cipitria"},{"first_name":"Rhoslyn A.","full_name":"Coles, Rhoslyn A.","last_name":"Coles"},{"first_name":"Rumiana","full_name":"Dimova, Rumiana","last_name":"Dimova"},{"last_name":"Eder","full_name":"Eder, Michaela","first_name":"Michaela"},{"full_name":"Ehrig, Sebastian","last_name":"Ehrig","first_name":"Sebastian"},{"first_name":"Luis M.","full_name":"Escudero, Luis M.","last_name":"Escudero"},{"full_name":"Evans, Myfanwy E.","last_name":"Evans","first_name":"Myfanwy E."},{"full_name":"Fernandes, Paulo R.","last_name":"Fernandes","first_name":"Paulo R."},{"first_name":"Peter","last_name":"Fratzl","full_name":"Fratzl, Peter"},{"last_name":"Geris","full_name":"Geris, Liesbet","first_name":"Liesbet"},{"first_name":"Notburga","full_name":"Gierlinger, Notburga","last_name":"Gierlinger"},{"full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"},{"first_name":"Aleš","last_name":"Iglič","full_name":"Iglič, Aleš"},{"first_name":"Jacob J.K.","full_name":"Kirkensgaard, Jacob J.K.","last_name":"Kirkensgaard"},{"first_name":"Philip","full_name":"Kollmannsberger, Philip","last_name":"Kollmannsberger"},{"last_name":"Kowalewska","full_name":"Kowalewska, Łucja","first_name":"Łucja"},{"first_name":"Nicholas A.","full_name":"Kurniawan, Nicholas A.","last_name":"Kurniawan"},{"last_name":"Papantoniou","full_name":"Papantoniou, Ioannis","first_name":"Ioannis"},{"last_name":"Pieuchot","full_name":"Pieuchot, Laurent","first_name":"Laurent"},{"first_name":"Tiago H.V.","full_name":"Pires, Tiago H.V.","last_name":"Pires"},{"first_name":"Lars D.","full_name":"Renner, Lars D.","last_name":"Renner"},{"first_name":"Andrew O.","last_name":"Sageman-Furnas","full_name":"Sageman-Furnas, Andrew O."},{"first_name":"Gerd E.","full_name":"Schröder-Turk, Gerd E.","last_name":"Schröder-Turk"},{"first_name":"Anupam","full_name":"Sengupta, Anupam","last_name":"Sengupta"},{"last_name":"Sharma","full_name":"Sharma, Vikas R.","first_name":"Vikas R."},{"first_name":"Antonio","full_name":"Tagua, Antonio","last_name":"Tagua"},{"last_name":"Tomba","full_name":"Tomba, Caterina","first_name":"Caterina"},{"first_name":"Xavier","last_name":"Trepat","full_name":"Trepat, Xavier"},{"first_name":"Sarah L.","full_name":"Waters, Sarah L.","last_name":"Waters"},{"first_name":"Edwina F.","last_name":"Yeo","full_name":"Yeo, Edwina F."},{"last_name":"Roschger","full_name":"Roschger, Andreas","first_name":"Andreas"},{"first_name":"Cécile M.","full_name":"Bidan, Cécile M.","last_name":"Bidan"},{"full_name":"Dunlop, John W.C.","last_name":"Dunlop","first_name":"John W.C."}],"article_processing_charge":"No","external_id":{"pmid":["36461812"],"isi":["000941068900001"]},"title":"Curvature in biological systems: Its quantification, emergence, and implications across the scales","acknowledgement":"B.S. and A.R. contributed equally to this work. A.P.G.C. and P.R.F. acknowledge the funding from Fundação para a Ciência e Tecnologia (Portugal), through IDMEC, under LAETA project UIDB/50022/2020. T.H.V.P. acknowledges the funding from Fundação para a Ciência e Tecnologia (Portugal), through Ph.D. Grant 2020.04417.BD. A.S. acknowledges that this work was partially supported by the ATTRACT Investigator Grant (no. A17/MS/11572821/MBRACE, to A.S.) from the Luxembourg National Research Fund. The author thanks Gerardo Ceada for his help in the graphical representations. N.A.K. acknowledges support from the European Research Council (grant 851960) and the Gravitation Program “Materials Driven Regeneration,” funded by the Netherlands Organization for Scientific Research (024.003.013). M.B.A. acknowledges support from the French National Research Agency (grant ANR-201-8-CE1-3-0008 for the project “Epimorph”). G.E.S.T. acknowledges funding by the Australian Research Council through project DP200102593. A.C. acknowledges the funding from the Deutsche Forschungsgemeinschaft (DFG) Emmy Noether Grant CI 203/-2 1, the Spanish Ministry of Science and Innovation (PID2021-123013O-BI00) and the IKERBASQUE Basque Foundation for Science.","quality_controlled":"1","publisher":"Wiley","oa":1,"has_accepted_license":"1","isi":1,"year":"2023","day":"29","publication":"Advanced Materials","doi":"10.1002/adma.202206110","date_published":"2023-03-29T00:00:00Z","date_created":"2023-03-05T23:01:06Z","_id":"12710","type":"journal_article","article_type":"review","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"status":"public","date_updated":"2023-09-26T10:56:46Z","ddc":["570"],"department":[{"_id":"EdHa"}],"file_date_updated":"2023-09-26T10:51:56Z","abstract":[{"text":"Surface curvature both emerges from, and influences the behavior of, living objects at length scales ranging from cell membranes to single cells to tissues and organs. The relevance of surface curvature in biology is supported by numerous experimental and theoretical investigations in recent years. In this review, first, a brief introduction to the key ideas of surface curvature in the context of biological systems is given and the challenges that arise when measuring surface curvature are discussed. Giving an overview of the emergence of curvature in biological systems, its significance at different length scales becomes apparent. On the other hand, summarizing current findings also shows that both single cells and entire cell sheets, tissues or organisms respond to curvature by modulating their shape and their migration behavior. Finally, the interplay between the distribution of morphogens or micro-organisms and the emergence of curvature across length scales is addressed with examples demonstrating these key mechanistic principles of morphogenesis. Overall, this review highlights that curved interfaces are not merely a passive by-product of the chemical, biological, and mechanical processes but that curvature acts also as a signal that co-determines these processes.","lang":"eng"}],"oa_version":"Published Version","pmid":1,"scopus_import":"1","month":"03","intvolume":" 35","publication_identifier":{"issn":["0935-9648"],"eissn":["1521-4095"]},"publication_status":"published","file":[{"access_level":"open_access","relation":"main_file","content_type":"application/pdf","checksum":"5c04d68130e97a0ecd1ca27fbc15a246","file_id":"14373","success":1,"creator":"dernst","date_updated":"2023-09-26T10:51:56Z","file_size":2898063,"date_created":"2023-09-26T10:51:56Z","file_name":"2023_AdvancedMaterials_Schamberger.pdf"}],"language":[{"iso":"eng"}],"issue":"13","volume":35},{"language":[{"iso":"eng"}],"file":[{"file_size":5532285,"date_updated":"2023-10-04T11:13:28Z","creator":"dernst","file_name":"2023_NaturePhysics_Boncanegra.pdf","date_created":"2023-10-04T11:13:28Z","content_type":"application/pdf","relation":"main_file","access_level":"open_access","success":1,"checksum":"858225a4205b74406e5045006cdd853f","file_id":"14392"}],"publication_status":"published","publication_identifier":{"eissn":["1745-2481"],"issn":["1745-2473"]},"ec_funded":1,"volume":19,"related_material":{"record":[{"id":"13081","status":"public","relation":"dissertation_contains"}]},"oa_version":"Published Version","abstract":[{"lang":"eng","text":"As developing tissues grow in size and undergo morphogenetic changes, their material properties may be altered. Such changes result from tension dynamics at cell contacts or cellular jamming. Yet, in many cases, the cellular mechanisms controlling the physical state of growing tissues are unclear. We found that at early developmental stages, the epithelium in the developing mouse spinal cord maintains both high junctional tension and high fluidity. This is achieved via a mechanism in which interkinetic nuclear movements generate cell area dynamics that drive extensive cell rearrangements. Over time, the cell proliferation rate declines, effectively solidifying the tissue. Thus, unlike well-studied jamming transitions, the solidification uncovered here resembles a glass transition that depends on the dynamical stresses generated by proliferation and differentiation. Our finding that the fluidity of developing epithelia is linked to interkinetic nuclear movements and the dynamics of growth is likely to be relevant to multiple developing tissues."}],"intvolume":" 19","month":"07","scopus_import":"1","ddc":["570"],"date_updated":"2023-10-04T11:14:05Z","department":[{"_id":"EdHa"},{"_id":"AnKi"}],"file_date_updated":"2023-10-04T11:13:28Z","_id":"12837","status":"public","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_type":"original","type":"journal_article","publication":"Nature Physics","day":"01","year":"2023","has_accepted_license":"1","isi":1,"date_created":"2023-04-16T22:01:09Z","doi":"10.1038/s41567-023-01977-w","date_published":"2023-07-01T00:00:00Z","page":"1050-1058","acknowledgement":"We thank S. Hippenmeyer for the reagents and C. P. Heisenberg, J. Briscoe and K. Page for comments on the manuscript. This work was supported by IST Austria; the European Research Council under Horizon 2020 research and innovation programme grant no. 680037 and Horizon Europe grant 101044579 (A.K.); Austrian Science Fund (FWF): F78 (Stem Cell Modulation) (A.K.); ISTFELLOW postdoctoral program (A.S.); Narodowe Centrum Nauki, Poland SONATA, 2017/26/D/NZ2/00454 (M.Z.); and the Polish National Agency for Academic Exchange (M.Z.).","oa":1,"publisher":"Springer Nature","quality_controlled":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"short":"L. Bocanegra, A. Singh, E.B. Hannezo, M.P. Zagórski, A. Kicheva, Nature Physics 19 (2023) 1050–1058.","ieee":"L. Bocanegra, A. Singh, E. B. Hannezo, M. P. Zagórski, and A. Kicheva, “Cell cycle dynamics control fluidity of the developing mouse neuroepithelium,” Nature Physics, vol. 19. Springer Nature, pp. 1050–1058, 2023.","ama":"Bocanegra L, Singh A, Hannezo EB, Zagórski MP, Kicheva A. Cell cycle dynamics control fluidity of the developing mouse neuroepithelium. Nature Physics. 2023;19:1050-1058. doi:10.1038/s41567-023-01977-w","apa":"Bocanegra, L., Singh, A., Hannezo, E. B., Zagórski, M. P., & Kicheva, A. (2023). Cell cycle dynamics control fluidity of the developing mouse neuroepithelium. Nature Physics. Springer Nature. https://doi.org/10.1038/s41567-023-01977-w","mla":"Bocanegra, Laura, et al. “Cell Cycle Dynamics Control Fluidity of the Developing Mouse Neuroepithelium.” Nature Physics, vol. 19, Springer Nature, 2023, pp. 1050–58, doi:10.1038/s41567-023-01977-w.","ista":"Bocanegra L, Singh A, Hannezo EB, Zagórski MP, Kicheva A. 2023. Cell cycle dynamics control fluidity of the developing mouse neuroepithelium. Nature Physics. 19, 1050–1058.","chicago":"Bocanegra, Laura, Amrita Singh, Edouard B Hannezo, Marcin P Zagórski, and Anna Kicheva. “Cell Cycle Dynamics Control Fluidity of the Developing Mouse Neuroepithelium.” Nature Physics. Springer Nature, 2023. https://doi.org/10.1038/s41567-023-01977-w."},"title":"Cell cycle dynamics control fluidity of the developing mouse neuroepithelium","external_id":{"isi":["000964029300003"]},"article_processing_charge":"No","author":[{"full_name":"Bocanegra, Laura","last_name":"Bocanegra","id":"4896F754-F248-11E8-B48F-1D18A9856A87","first_name":"Laura"},{"last_name":"Singh","full_name":"Singh, Amrita","first_name":"Amrita","id":"76250f9f-3a21-11eb-9a80-a6180a0d7958"},{"full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0001-7896-7762","full_name":"Zagórski, Marcin P","last_name":"Zagórski","first_name":"Marcin P","id":"343DA0DC-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0003-4509-4998","full_name":"Kicheva, Anna","last_name":"Kicheva","first_name":"Anna","id":"3959A2A0-F248-11E8-B48F-1D18A9856A87"}],"project":[{"name":"Coordination of Patterning And Growth In the Spinal Cord","grant_number":"680037","call_identifier":"H2020","_id":"B6FC0238-B512-11E9-945C-1524E6697425"},{"_id":"bd7e737f-d553-11ed-ba76-d69ffb5ee3aa","grant_number":"101044579","name":"Mechanisms of tissue size regulation in spinal cord development"},{"grant_number":"F07802","name":"Morphogen control of growth and pattern in the spinal cord","_id":"059DF620-7A3F-11EA-A408-12923DDC885E"},{"_id":"25681D80-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","name":"International IST Postdoc Fellowship Programme","grant_number":"291734"}]},{"_id":"14426","status":"public","article_type":"original","type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"ddc":["570"],"date_updated":"2023-10-16T07:25:48Z","file_date_updated":"2023-10-16T07:20:49Z","department":[{"_id":"EdHa"}],"oa_version":"Published Version","abstract":[{"text":"To meet the physiological demands of the body, organs need to establish a functional tissue architecture and adequate size as the embryo develops to adulthood. In the liver, uni- and bipotent progenitor differentiation into hepatocytes and biliary epithelial cells (BECs), and their relative proportions, comprise the functional architecture. Yet, the contribution of individual liver progenitors at the organ level to both fates, and their specific proportion, is unresolved. Combining mathematical modelling with organ-wide, multispectral FRaeppli-NLS lineage tracing in zebrafish, we demonstrate that a precise BEC-to-hepatocyte ratio is established (i) fast, (ii) solely by heterogeneous lineage decisions from uni- and bipotent progenitors, and (iii) independent of subsequent cell type–specific proliferation. Extending lineage tracing to adulthood determined that embryonic cells undergo spatially heterogeneous three-dimensional growth associated with distinct environments. Strikingly, giant clusters comprising almost half a ventral lobe suggest lobe-specific dominant-like growth behaviours. We show substantial hepatocyte polyploidy in juveniles representing another hallmark of postembryonic liver growth. Our findings uncover heterogeneous progenitor contributions to tissue architecture-defining cell type proportions and postembryonic organ growth as key mechanisms forming the adult liver.","lang":"eng"}],"month":"10","intvolume":" 21","scopus_import":"1","file":[{"creator":"dernst","file_size":6193110,"date_updated":"2023-10-16T07:20:49Z","file_name":"2023_PloSBiology_Unterweger.pdf","date_created":"2023-10-16T07:20:49Z","relation":"main_file","access_level":"open_access","content_type":"application/pdf","success":1,"checksum":"40a2b11b41d70a0e5939f8a52b66e389","file_id":"14431"}],"language":[{"iso":"eng"}],"publication_identifier":{"eissn":["1545-7885"]},"publication_status":"published","issue":"10","related_material":{"link":[{"relation":"software","url":"https://github.com/JulieKlepstad/LiverDevelopment"}]},"volume":21,"ec_funded":1,"article_number":"e3002315","project":[{"_id":"05943252-7A3F-11EA-A408-12923DDC885E","call_identifier":"H2020","name":"Design Principles of Branching Morphogenesis","grant_number":"851288"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"chicago":"Unterweger, Iris A., Julie Klepstad, Edouard B Hannezo, Pia R. Lundegaard, Ala Trusina, and Elke A. Ober. “Lineage Tracing Identifies Heterogeneous Hepatoblast Contribution to Cell Lineages and Postembryonic Organ Growth Dynamics.” PLoS Biology. Public Library of Science, 2023. https://doi.org/10.1371/journal.pbio.3002315.","ista":"Unterweger IA, Klepstad J, Hannezo EB, Lundegaard PR, Trusina A, Ober EA. 2023. Lineage tracing identifies heterogeneous hepatoblast contribution to cell lineages and postembryonic organ growth dynamics. PLoS Biology. 21(10), e3002315.","mla":"Unterweger, Iris A., et al. “Lineage Tracing Identifies Heterogeneous Hepatoblast Contribution to Cell Lineages and Postembryonic Organ Growth Dynamics.” PLoS Biology, vol. 21, no. 10, e3002315, Public Library of Science, 2023, doi:10.1371/journal.pbio.3002315.","short":"I.A. Unterweger, J. Klepstad, E.B. Hannezo, P.R. Lundegaard, A. Trusina, E.A. Ober, PLoS Biology 21 (2023).","ieee":"I. A. Unterweger, J. Klepstad, E. B. Hannezo, P. R. Lundegaard, A. Trusina, and E. A. Ober, “Lineage tracing identifies heterogeneous hepatoblast contribution to cell lineages and postembryonic organ growth dynamics,” PLoS Biology, vol. 21, no. 10. Public Library of Science, 2023.","apa":"Unterweger, I. A., Klepstad, J., Hannezo, E. B., Lundegaard, P. R., Trusina, A., & Ober, E. A. (2023). Lineage tracing identifies heterogeneous hepatoblast contribution to cell lineages and postembryonic organ growth dynamics. PLoS Biology. Public Library of Science. https://doi.org/10.1371/journal.pbio.3002315","ama":"Unterweger IA, Klepstad J, Hannezo EB, Lundegaard PR, Trusina A, Ober EA. Lineage tracing identifies heterogeneous hepatoblast contribution to cell lineages and postembryonic organ growth dynamics. PLoS Biology. 2023;21(10). doi:10.1371/journal.pbio.3002315"},"title":"Lineage tracing identifies heterogeneous hepatoblast contribution to cell lineages and postembryonic organ growth dynamics","author":[{"last_name":"Unterweger","full_name":"Unterweger, Iris A.","first_name":"Iris A."},{"full_name":"Klepstad, Julie","last_name":"Klepstad","first_name":"Julie"},{"orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","last_name":"Hannezo","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Lundegaard","full_name":"Lundegaard, Pia R.","first_name":"Pia R."},{"first_name":"Ala","full_name":"Trusina, Ala","last_name":"Trusina"},{"first_name":"Elke A.","full_name":"Ober, Elke A.","last_name":"Ober"}],"article_processing_charge":"No","acknowledgement":"We thank the Ober group for discussion and comments on the manuscript. We are grateful to\r\nDr. F. Lemaigre for feedback on the manuscript and Dr. T. Piotrowski for invaluable support.\r\nWe thank the department of experimental medicine (AEM) in Copenhagen for expert fish\r\ncare. We gratefully acknowledge the DanStem Imaging Platform (University of Copenhagen)\r\nfor support and assistance in this work.\r\nThis work is supported by Novo Nordisk Foundation grant NNF17CC0027852 (EAO);\r\nNordisk Foundation grant NNF19OC0058327 (EAO); Novo Nordisk Foundation grant\r\nNNF17OC0031204 (PRL); https://novonordiskfonden.dk/en/; Danish National\r\nResearch Foundation grant DNRF116 (EAO and AT); https://dg.dk/en/; John and Birthe Meyer\r\nFoundation (PRL) and European Research Council (ERC) under the EU Horizon 2020 research and Innovation Programme Grant Agreement No. 851288 (EH).","quality_controlled":"1","publisher":"Public Library of Science","oa":1,"day":"04","publication":"PLoS Biology","has_accepted_license":"1","year":"2023","date_published":"2023-10-04T00:00:00Z","doi":"10.1371/journal.pbio.3002315","date_created":"2023-10-15T22:01:10Z"},{"date_updated":"2023-12-13T12:31:05Z","ddc":["570"],"file_date_updated":"2023-10-03T07:46:36Z","department":[{"_id":"EdHa"}],"_id":"14378","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"type":"journal_article","article_type":"original","status":"public","publication_status":"published","publication_identifier":{"eissn":["2041-1723"]},"language":[{"iso":"eng"}],"file":[{"creator":"dernst","date_updated":"2023-10-03T07:46:36Z","file_size":8143264,"date_created":"2023-10-03T07:46:36Z","file_name":"2023_NatureComm_Ucar.pdf","access_level":"open_access","relation":"main_file","content_type":"application/pdf","file_id":"14384","checksum":"4fe5423403f2531753bcd9e0fea48e05","success":1}],"ec_funded":1,"volume":14,"abstract":[{"lang":"eng","text":"Branching morphogenesis is a ubiquitous process that gives rise to high exchange surfaces in the vasculature and epithelial organs. Lymphatic capillaries form branched networks, which play a key role in the circulation of tissue fluid and immune cells. Although mouse models and correlative patient data indicate that the lymphatic capillary density directly correlates with functional output, i.e., tissue fluid drainage and trafficking efficiency of dendritic cells, the mechanisms ensuring efficient tissue coverage remain poorly understood. Here, we use the mouse ear pinna lymphatic vessel network as a model system and combine lineage-tracing, genetic perturbations, whole-organ reconstructions and theoretical modeling to show that the dermal lymphatic capillaries tile space in an optimal, space-filling manner. This coverage is achieved by two complementary mechanisms: initial tissue invasion provides a non-optimal global scaffold via self-organized branching morphogenesis, while VEGF-C dependent side-branching from existing capillaries rapidly optimizes local coverage by directionally targeting low-density regions. With these two ingredients, we show that a minimal biophysical model can reproduce quantitatively whole-network reconstructions, across development and perturbations. Our results show that lymphatic capillary networks can exploit local self-organizing mechanisms to achieve tissue-scale optimization."}],"oa_version":"Published Version","pmid":1,"scopus_import":"1","intvolume":" 14","month":"09","citation":{"ista":"Ucar MC, Hannezo EB, Tiilikainen E, Liaqat I, Jakobsson E, Nurmi H, Vaahtomeri K. 2023. Self-organized and directed branching results in optimal coverage in developing dermal lymphatic networks. Nature Communications. 14, 5878.","chicago":"Ucar, Mehmet C, Edouard B Hannezo, Emmi Tiilikainen, Inam Liaqat, Emma Jakobsson, Harri Nurmi, and Kari Vaahtomeri. “Self-Organized and Directed Branching Results in Optimal Coverage in Developing Dermal Lymphatic Networks.” Nature Communications. Springer Nature, 2023. https://doi.org/10.1038/s41467-023-41456-7.","apa":"Ucar, M. C., Hannezo, E. B., Tiilikainen, E., Liaqat, I., Jakobsson, E., Nurmi, H., & Vaahtomeri, K. (2023). Self-organized and directed branching results in optimal coverage in developing dermal lymphatic networks. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-023-41456-7","ama":"Ucar MC, Hannezo EB, Tiilikainen E, et al. Self-organized and directed branching results in optimal coverage in developing dermal lymphatic networks. Nature Communications. 2023;14. doi:10.1038/s41467-023-41456-7","short":"M.C. Ucar, E.B. Hannezo, E. Tiilikainen, I. Liaqat, E. Jakobsson, H. Nurmi, K. Vaahtomeri, Nature Communications 14 (2023).","ieee":"M. C. Ucar et al., “Self-organized and directed branching results in optimal coverage in developing dermal lymphatic networks,” Nature Communications, vol. 14. Springer Nature, 2023.","mla":"Ucar, Mehmet C., et al. “Self-Organized and Directed Branching Results in Optimal Coverage in Developing Dermal Lymphatic Networks.” Nature Communications, vol. 14, 5878, Springer Nature, 2023, doi:10.1038/s41467-023-41456-7."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_processing_charge":"Yes","external_id":{"pmid":["37735168"],"isi":["001075884500007"]},"author":[{"last_name":"Ucar","orcid":"0000-0003-0506-4217","full_name":"Ucar, Mehmet C","first_name":"Mehmet C","id":"50B2A802-6007-11E9-A42B-EB23E6697425"},{"last_name":"Hannezo","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"},{"full_name":"Tiilikainen, Emmi","last_name":"Tiilikainen","first_name":"Emmi"},{"first_name":"Inam","full_name":"Liaqat, Inam","last_name":"Liaqat"},{"last_name":"Jakobsson","full_name":"Jakobsson, Emma","first_name":"Emma"},{"last_name":"Nurmi","full_name":"Nurmi, Harri","first_name":"Harri"},{"last_name":"Vaahtomeri","orcid":"0000-0001-7829-3518","full_name":"Vaahtomeri, Kari","id":"368EE576-F248-11E8-B48F-1D18A9856A87","first_name":"Kari"}],"title":"Self-organized and directed branching results in optimal coverage in developing dermal lymphatic networks","article_number":"5878","project":[{"name":"Design Principles of Branching Morphogenesis","grant_number":"851288","call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E"},{"_id":"260C2330-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"754411","name":"ISTplus - Postdoctoral Fellowships"}],"year":"2023","has_accepted_license":"1","isi":1,"publication":"Nature Communications","day":"21","date_created":"2023-10-01T22:01:13Z","date_published":"2023-09-21T00:00:00Z","doi":"10.1038/s41467-023-41456-7","acknowledgement":"We thank Dr. Kari Alitalo (University of Helsinki and Wihuri Research Institute) for critical reading of the manuscript, providing Vegfc+/− and Clp24ΔEC mouse strains and for hosting K.V.’s Academy of Finland postdoctoral researcher period (2015–2018). We thank Dr. Sara Wickström (University of Helsinki and Wihuri Research Institute) for providing Sox9:Egfp mouse\r\nstrain and the discussions. We thank Maija Atuegwu and Tapio Tainola for technical assistance. This work received funding from the Academy of Finland (K.V., 315710), Sigrid Juselius Foundation (K.V.), University of Helsinki (K.V.), Wihuri Research Institute (K.V.), the ERC under the European Union’s Horizon 2020 research and innovation program (grant agreement\r\nNo. 851288 to E.H.) and under the Marie Skłodowska-Curie grant agreement No. 754411 (to M.C.U.). Part of the work was carried out with the support of HiLIFE Laboratory Animal Centre Core Facility, University of Helsinki, Finland. Imaging was performed at the Biomedicum Imaging Unit, Helsinki University, Helsinki, Finland, with the support of Biocenter Finland. The AAVpreparations were produced at the Helsinki Virus (HelVi) Core.","oa":1,"publisher":"Springer Nature","quality_controlled":"1"},{"acknowledgement":"We thank I. de Vries and the Scientific Service Units (Life Sciences, Bioimaging, Nanofabrication, Preclinical and Miba Machine Shop) of the Institute of Science and Technology Austria for excellent support, as well as all the rotation students assisting in the laboratory work (B. Zens, H. Schön, and D. Babic).\r\nThis work was supported by grants from the European Research Council under the European Union’s Horizon 2020 research to M.S. (grant agreement no. 724373) and to E.H. (grant agreement no. 851288), and a grant by the Austrian Science Fund (DK Nanocell W1250-B20) to M.S. J.A. was supported by the Jenny and Antti Wihuri Foundation and Research Council of Finland's Flagship Programme InFLAMES (decision number: 357910). M.C.U. was supported by the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 754411.","quality_controlled":"1","publisher":"American Association for the Advancement of Science","oa":1,"isi":1,"year":"2023","day":"01","publication":"Science Immunology","date_published":"2023-09-01T00:00:00Z","doi":"10.1126/sciimmunol.adc9584","date_created":"2023-09-06T08:07:51Z","article_number":"adc9584","project":[{"grant_number":"724373","name":"Cellular navigation along spatial gradients","_id":"25FE9508-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"},{"name":"Design Principles of Branching Morphogenesis","grant_number":"851288","call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E"},{"name":"Nano-Analytics of Cellular Systems","grant_number":"W01250-B20","_id":"265E2996-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"},{"_id":"260C2330-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"ISTplus - Postdoctoral Fellowships","grant_number":"754411"}],"citation":{"mla":"Alanko, Jonna H., et al. “CCR7 Acts as Both a Sensor and a Sink for CCL19 to Coordinate Collective Leukocyte Migration.” Science Immunology, vol. 8, no. 87, adc9584, American Association for the Advancement of Science, 2023, doi:10.1126/sciimmunol.adc9584.","ama":"Alanko JH, Ucar MC, Canigova N, et al. CCR7 acts as both a sensor and a sink for CCL19 to coordinate collective leukocyte migration. Science Immunology. 2023;8(87). doi:10.1126/sciimmunol.adc9584","apa":"Alanko, J. H., Ucar, M. C., Canigova, N., Stopp, J. A., Schwarz, J., Merrin, J., … Sixt, M. K. (2023). CCR7 acts as both a sensor and a sink for CCL19 to coordinate collective leukocyte migration. Science Immunology. American Association for the Advancement of Science. https://doi.org/10.1126/sciimmunol.adc9584","short":"J.H. Alanko, M.C. Ucar, N. Canigova, J.A. Stopp, J. Schwarz, J. Merrin, E.B. Hannezo, M.K. Sixt, Science Immunology 8 (2023).","ieee":"J. H. Alanko et al., “CCR7 acts as both a sensor and a sink for CCL19 to coordinate collective leukocyte migration,” Science Immunology, vol. 8, no. 87. American Association for the Advancement of Science, 2023.","chicago":"Alanko, Jonna H, Mehmet C Ucar, Nikola Canigova, Julian A Stopp, Jan Schwarz, Jack Merrin, Edouard B Hannezo, and Michael K Sixt. “CCR7 Acts as Both a Sensor and a Sink for CCL19 to Coordinate Collective Leukocyte Migration.” Science Immunology. American Association for the Advancement of Science, 2023. https://doi.org/10.1126/sciimmunol.adc9584.","ista":"Alanko JH, Ucar MC, Canigova N, Stopp JA, Schwarz J, Merrin J, Hannezo EB, Sixt MK. 2023. CCR7 acts as both a sensor and a sink for CCL19 to coordinate collective leukocyte migration. Science Immunology. 8(87), adc9584."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"first_name":"Jonna H","id":"2CC12E8C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7698-3061","full_name":"Alanko, Jonna H","last_name":"Alanko"},{"id":"50B2A802-6007-11E9-A42B-EB23E6697425","first_name":"Mehmet C","full_name":"Ucar, Mehmet C","orcid":"0000-0003-0506-4217","last_name":"Ucar"},{"first_name":"Nikola","id":"3795523E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8518-5926","full_name":"Canigova, Nikola","last_name":"Canigova"},{"first_name":"Julian A","id":"489E3F00-F248-11E8-B48F-1D18A9856A87","last_name":"Stopp","full_name":"Stopp, Julian A"},{"id":"346C1EC6-F248-11E8-B48F-1D18A9856A87","first_name":"Jan","last_name":"Schwarz","full_name":"Schwarz, Jan"},{"last_name":"Merrin","orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Hannezo","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"},{"full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","last_name":"Sixt","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"}],"external_id":{"pmid":["37656776"],"isi":["001062110600003"]},"article_processing_charge":"No","title":"CCR7 acts as both a sensor and a sink for CCL19 to coordinate collective leukocyte migration","abstract":[{"lang":"eng","text":"Immune responses rely on the rapid and coordinated migration of leukocytes. Whereas it is well established that single-cell migration is often guided by gradients of chemokines and other chemoattractants, it remains poorly understood how these gradients are generated, maintained, and modulated. By combining experimental data with theory on leukocyte chemotaxis guided by the G protein–coupled receptor (GPCR) CCR7, we demonstrate that in addition to its role as the sensory receptor that steers migration, CCR7 also acts as a generator and a modulator of chemotactic gradients. Upon exposure to the CCR7 ligand CCL19, dendritic cells (DCs) effectively internalize the receptor and ligand as part of the canonical GPCR desensitization response. We show that CCR7 internalization also acts as an effective sink for the chemoattractant, dynamically shaping the spatiotemporal distribution of the chemokine. This mechanism drives complex collective migration patterns, enabling DCs to create or sharpen chemotactic gradients. We further show that these self-generated gradients can sustain the long-range guidance of DCs, adapt collective migration patterns to the size and geometry of the environment, and provide a guidance cue for other comigrating cells. Such a dual role of CCR7 as a GPCR that both senses and consumes its ligand can thus provide a novel mode of cellular self-organization."}],"pmid":1,"oa_version":"Published Version","scopus_import":"1","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1126/sciimmunol.adc9584"}],"month":"09","intvolume":" 8","publication_identifier":{"issn":["2470-9468"]},"publication_status":"published","language":[{"iso":"eng"}],"volume":8,"issue":"87","related_material":{"record":[{"status":"public","id":"14279","relation":"research_data"},{"relation":"dissertation_contains","id":"14697","status":"public"}]},"ec_funded":1,"_id":"14274","type":"journal_article","article_type":"original","status":"public","keyword":["General Medicine","Immunology"],"date_updated":"2023-12-21T14:30:01Z","department":[{"_id":"MiSi"},{"_id":"EdHa"},{"_id":"NanoFab"}]},{"abstract":[{"lang":"eng","text":"Homeostatic balance in the intestinal epithelium relies on a fast cellular turnover, which is coordinated by an intricate interplay between biochemical signalling, mechanical forces and organ geometry. We review recent modelling approaches that have been developed to understand different facets of this remarkable homeostatic equilibrium. Existing models offer different, albeit complementary, perspectives on the problem. First, biomechanical models aim to explain the local and global mechanical stresses driving cell renewal as well as tissue shape maintenance. Second, compartmental models provide insights into the conditions necessary to keep a constant flow of cells with well-defined ratios of cell types, and how perturbations can lead to an unbalance of relative compartment sizes. A third family of models address, at the cellular level, the nature and regulation of stem fate choices that are necessary to fuel cellular turnover. We also review how these different approaches are starting to be integrated together across scales, to provide quantitative predictions and new conceptual frameworks to think about the dynamics of cell renewal in complex tissues."}],"oa_version":"Published Version","pmid":1,"scopus_import":"1","month":"12","publication_status":"published","publication_identifier":{"issn":["1084-9521"]},"language":[{"iso":"eng"}],"file":[{"file_name":"2023_SeminarsCellDevBiology_CorominasMurtra.pdf","date_created":"2024-01-08T10:16:04Z","creator":"dernst","file_size":1343750,"date_updated":"2024-01-08T10:16:04Z","success":1,"file_id":"14741","checksum":"c619887cf130f4649bf3035417186004","relation":"main_file","access_level":"open_access","content_type":"application/pdf"}],"ec_funded":1,"volume":"150-151","_id":"12162","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"type":"journal_article","article_type":"review","keyword":["Cell Biology","Developmental Biology"],"status":"public","date_updated":"2024-01-16T13:22:32Z","ddc":["570"],"file_date_updated":"2024-01-08T10:16:04Z","department":[{"_id":"EdHa"}],"acknowledgement":"This work received funding from the ERC under the European Union’s Horizon 2020 research and innovation programme (grant agreement No. 851288 to E.H.).\r\nB. C-M wants to acknowledge the support of the field of excellence Complexity of Life, in Basic Research and Innovation of the University of Graz.","oa":1,"publisher":"Elsevier","quality_controlled":"1","year":"2023","has_accepted_license":"1","isi":1,"publication":"Seminars in Cell & Developmental Biology","day":"02","page":"58-65","date_created":"2023-01-12T12:09:47Z","doi":"10.1016/j.semcdb.2022.11.005","date_published":"2023-12-02T00:00:00Z","project":[{"grant_number":"851288","name":"Design Principles of Branching Morphogenesis","_id":"05943252-7A3F-11EA-A408-12923DDC885E","call_identifier":"H2020"}],"citation":{"ieee":"B. Corominas-Murtra and E. B. Hannezo, “Modelling the dynamics of mammalian gut homeostasis,” Seminars in Cell & Developmental Biology, vol. 150–151. Elsevier, pp. 58–65, 2023.","short":"B. Corominas-Murtra, E.B. Hannezo, Seminars in Cell & Developmental Biology 150–151 (2023) 58–65.","apa":"Corominas-Murtra, B., & Hannezo, E. B. (2023). Modelling the dynamics of mammalian gut homeostasis. Seminars in Cell & Developmental Biology. Elsevier. https://doi.org/10.1016/j.semcdb.2022.11.005","ama":"Corominas-Murtra B, Hannezo EB. Modelling the dynamics of mammalian gut homeostasis. Seminars in Cell & Developmental Biology. 2023;150-151:58-65. doi:10.1016/j.semcdb.2022.11.005","mla":"Corominas-Murtra, Bernat, and Edouard B. Hannezo. “Modelling the Dynamics of Mammalian Gut Homeostasis.” Seminars in Cell & Developmental Biology, vol. 150–151, Elsevier, 2023, pp. 58–65, doi:10.1016/j.semcdb.2022.11.005.","ista":"Corominas-Murtra B, Hannezo EB. 2023. Modelling the dynamics of mammalian gut homeostasis. Seminars in Cell & Developmental Biology. 150–151, 58–65.","chicago":"Corominas-Murtra, Bernat, and Edouard B Hannezo. “Modelling the Dynamics of Mammalian Gut Homeostasis.” Seminars in Cell & Developmental Biology. Elsevier, 2023. https://doi.org/10.1016/j.semcdb.2022.11.005."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_processing_charge":"Yes (via OA deal)","external_id":{"isi":["001053522200001"],"pmid":["36470715"]},"author":[{"orcid":"0000-0001-9806-5643","full_name":"Corominas-Murtra, Bernat","last_name":"Corominas-Murtra","first_name":"Bernat","id":"43BE2298-F248-11E8-B48F-1D18A9856A87"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","last_name":"Hannezo"}],"title":"Modelling the dynamics of mammalian gut homeostasis"},{"publication_status":"published","publication_identifier":{"issn":["1745-2473"],"eissn":["1745-2481"]},"language":[{"iso":"eng"}],"file":[{"file_size":6365607,"date_updated":"2024-01-30T12:26:08Z","creator":"dernst","file_name":"2023_NaturePhysics_Grober.pdf","date_created":"2024-01-30T12:26:08Z","content_type":"application/pdf","relation":"main_file","access_level":"open_access","success":1,"checksum":"7e282c2ebc0ac82125a04f6b4742d4c1","file_id":"14906"}],"ec_funded":1,"volume":19,"abstract":[{"lang":"eng","text":"When in equilibrium, thermal forces agitate molecules, which then diffuse, collide and bind to form materials. However, the space of accessible structures in which micron-scale particles can be organized by thermal forces is limited, owing to the slow dynamics and metastable states. Active agents in a passive fluid generate forces and flows, forming a bath with active fluctuations. Two unanswered questions are whether those active agents can drive the assembly of passive components into unconventional states and which material properties they will exhibit. Here we show that passive, sticky beads immersed in a bath of swimming Escherichia coli bacteria aggregate into unconventional clusters and gels that are controlled by the activity of the bath. We observe a slow but persistent rotation of the aggregates that originates in the chirality of the E. coli flagella and directs aggregation into structures that are not accessible thermally. We elucidate the aggregation mechanism with a numerical model of spinning, sticky beads and reproduce quantitatively the experimental results. We show that internal activity controls the phase diagram and the structure of the aggregates. Overall, our results highlight the promising role of active baths in designing the structural and mechanical properties of materials with unconventional phases."}],"oa_version":"Published Version","scopus_import":"1","intvolume":" 19","month":"11","date_updated":"2024-01-30T12:26:55Z","ddc":["530"],"file_date_updated":"2024-01-30T12:26:08Z","department":[{"_id":"EdHa"},{"_id":"AnSa"},{"_id":"JePa"}],"_id":"13971","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_type":"original","type":"journal_article","status":"public","year":"2023","isi":1,"has_accepted_license":"1","publication":"Nature Physics","day":"01","page":"1680-1688","date_created":"2023-08-06T22:01:11Z","date_published":"2023-11-01T00:00:00Z","doi":"10.1038/s41567-023-02136-x","acknowledgement":"D.G. and J.P. thank E. Krasnopeeva, C. Guet, G. Guessous and T. Hwa for providing the E. coli strains. This material is based upon work supported by the US Department of Energy under award DE-SC0019769. I.P. acknowledges funding by the European Union’s Horizon 2020 research and innovation programme under Marie Skłodowska-Curie Grant Agreement No. 101034413. A.Š. acknowledges funding from the European Research Council under the European Union’s Horizon 2020 research and innovation programme (Grant No. 802960). M.C.U. acknowledges funding from the European Union’s Horizon 2020 research and innovation programme under Marie Skłodowska-Curie Grant Agreement No. 754411.","oa":1,"publisher":"Springer Nature","quality_controlled":"1","citation":{"chicago":"Grober, Daniel, Ivan Palaia, Mehmet C Ucar, Edouard B Hannezo, Anđela Šarić, and Jérémie A Palacci. “Unconventional Colloidal Aggregation in Chiral Bacterial Baths.” Nature Physics. Springer Nature, 2023. https://doi.org/10.1038/s41567-023-02136-x.","ista":"Grober D, Palaia I, Ucar MC, Hannezo EB, Šarić A, Palacci JA. 2023. Unconventional colloidal aggregation in chiral bacterial baths. Nature Physics. 19, 1680–1688.","mla":"Grober, Daniel, et al. “Unconventional Colloidal Aggregation in Chiral Bacterial Baths.” Nature Physics, vol. 19, Springer Nature, 2023, pp. 1680–88, doi:10.1038/s41567-023-02136-x.","apa":"Grober, D., Palaia, I., Ucar, M. C., Hannezo, E. B., Šarić, A., & Palacci, J. A. (2023). Unconventional colloidal aggregation in chiral bacterial baths. Nature Physics. Springer Nature. https://doi.org/10.1038/s41567-023-02136-x","ama":"Grober D, Palaia I, Ucar MC, Hannezo EB, Šarić A, Palacci JA. Unconventional colloidal aggregation in chiral bacterial baths. Nature Physics. 2023;19:1680-1688. doi:10.1038/s41567-023-02136-x","short":"D. Grober, I. Palaia, M.C. Ucar, E.B. Hannezo, A. Šarić, J.A. Palacci, Nature Physics 19 (2023) 1680–1688.","ieee":"D. Grober, I. Palaia, M. C. Ucar, E. B. Hannezo, A. Šarić, and J. A. Palacci, “Unconventional colloidal aggregation in chiral bacterial baths,” Nature Physics, vol. 19. Springer Nature, pp. 1680–1688, 2023."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_processing_charge":"Yes","external_id":{"isi":["001037346400005"]},"author":[{"id":"abdfc56f-34fb-11ee-bd33-fd766fce5a99","first_name":"Daniel","full_name":"Grober, Daniel","last_name":"Grober"},{"id":"9c805cd2-4b75-11ec-a374-db6dd0ed57fa","first_name":"Ivan","last_name":"Palaia","orcid":" 0000-0002-8843-9485 ","full_name":"Palaia, Ivan"},{"full_name":"Ucar, Mehmet C","orcid":"0000-0003-0506-4217","last_name":"Ucar","id":"50B2A802-6007-11E9-A42B-EB23E6697425","first_name":"Mehmet C"},{"last_name":"Hannezo","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Šarić, Anđela","orcid":"0000-0002-7854-2139","last_name":"Šarić","first_name":"Anđela","id":"bf63d406-f056-11eb-b41d-f263a6566d8b"},{"orcid":"0000-0002-7253-9465","full_name":"Palacci, Jérémie A","last_name":"Palacci","first_name":"Jérémie 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emergence of large-scale order in self-organized systems relies on local interactions between individual components. During bacterial cell division, FtsZ -- a prokaryotic homologue of the eukaryotic protein tubulin -- polymerizes into treadmilling filaments that further organize into a cytoskeletal ring. In vitro, FtsZ filaments can form dynamic chiral assemblies. However, how the active and passive properties of individual filaments relate to these large-scale self-organized structures remains poorly understood. Here, we connect single filament properties with the mesoscopic scale by combining minimal active matter simulations and biochemical reconstitution experiments. We show that density and flexibility of active chiral filaments define their global order. At intermediate densities, curved, flexible filaments organize into chiral rings and polar bands. An effectively nematic organization dominates for high densities and for straight, mutant filaments with increased rigidity. Our predicted phase diagram captures these features quantitatively, demonstrating how the flexibility, density and chirality of active filaments affect their collective behaviour. Our findings shed light on the fundamental properties of active chiral matter and explain how treadmilling FtsZ filaments organize during bacterial cell division. "}],"acknowledgement":"This work was supported by the European Research Council through grant ERC 2015-StG-679239 and by the Austrian Science Fund (FWF) StandAlone P34607 to M.L., B. P.M. was also supported by the Kanazawa University WPI- NanoLSI Bio-SPM collaborative research program. Z.D. has received funding from Doctoral Programme of the Austrian Academy of Sciences (OeAW): Grant agreement 26360. We thank Jan Brugues (MPI CBG, Dresden, Germany), Andela Saric (ISTA, Klosterneuburg, Austria), Daniel Pearce (Uni Geneva, Switzerland) for valuable scientific input and comments on the manuscript. We are also thankful for the support by the Scientific Service Units (SSU) of IST Austria through resources provided by the Imaging and Optics Facility (IOF) and the Lab Support Facility (LSF). ","oa_version":"Published Version","oa":1,"publisher":"Institute of Science and Technology Austria","month":"07","date_updated":"2024-02-21T12:19:09Z","citation":{"short":"Z. Dunajova, B. Prats Mateu, P. Radler, K. Lim, D. Brandis, P. Velicky, J.G. Danzl, R.W. Wong, J. Elgeti, E.B. Hannezo, M. Loose, (2023).","ieee":"Z. Dunajova et al., “Chiral and nematic phases of flexible active filaments.” Institute of Science and Technology Austria, 2023.","ama":"Dunajova Z, Prats Mateu B, Radler P, et al. Chiral and nematic phases of flexible active filaments. 2023. doi:10.15479/AT:ISTA:13116","apa":"Dunajova, Z., Prats Mateu, B., Radler, P., Lim, K., Brandis, D., Velicky, P., … Loose, M. (2023). Chiral and nematic phases of flexible active filaments. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:13116","mla":"Dunajova, Zuzana, et al. Chiral and Nematic Phases of Flexible Active Filaments. Institute of Science and Technology Austria, 2023, doi:10.15479/AT:ISTA:13116.","ista":"Dunajova Z, Prats Mateu B, Radler P, Lim K, Brandis D, Velicky P, Danzl JG, Wong RW, Elgeti J, Hannezo EB, Loose M. 2023. Chiral and nematic phases of flexible active filaments, Institute of Science and Technology Austria, 10.15479/AT:ISTA:13116.","chicago":"Dunajova, Zuzana, Batirtze Prats Mateu, Philipp Radler, Keesiang Lim, Dörte Brandis, Philipp Velicky, Johann G Danzl, et al. “Chiral and Nematic Phases of Flexible Active Filaments.” Institute of Science and Technology Austria, 2023. https://doi.org/10.15479/AT:ISTA:13116."},"ddc":["539"],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_processing_charge":"No","author":[{"last_name":"Dunajova","full_name":"Dunajova, Zuzana","id":"4B39F286-F248-11E8-B48F-1D18A9856A87","first_name":"Zuzana"},{"last_name":"Prats Mateu","full_name":"Prats Mateu, Batirtze","first_name":"Batirtze","id":"299FE892-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Radler","orcid":"0000-0001-9198-2182 ","full_name":"Radler, Philipp","id":"40136C2A-F248-11E8-B48F-1D18A9856A87","first_name":"Philipp"},{"first_name":"Keesiang","last_name":"Lim","full_name":"Lim, Keesiang"},{"first_name":"Dörte","last_name":"Brandis","full_name":"Brandis, Dörte"},{"first_name":"Philipp","id":"39BDC62C-F248-11E8-B48F-1D18A9856A87","last_name":"Velicky","orcid":"0000-0002-2340-7431","full_name":"Velicky, Philipp"},{"first_name":"Johann G","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","last_name":"Danzl","orcid":"0000-0001-8559-3973","full_name":"Danzl, Johann G"},{"first_name":"Richard W.","full_name":"Wong, Richard W.","last_name":"Wong"},{"full_name":"Elgeti, Jens","last_name":"Elgeti","first_name":"Jens"},{"first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo"},{"first_name":"Martin","id":"462D4284-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7309-9724","full_name":"Loose, Martin","last_name":"Loose"}],"department":[{"_id":"MaLo"},{"_id":"EdHa"},{"_id":"JoDa"}],"file_date_updated":"2023-08-08T11:17:28Z","title":"Chiral and nematic phases of flexible active filaments","_id":"13116","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"type":"research_data","status":"public","project":[{"call_identifier":"H2020","_id":"2595697A-B435-11E9-9278-68D0E5697425","name":"Self-Organization of the Bacterial Cell","grant_number":"679239"},{"name":"Understanding bacterial cell division by in vitro\r\nreconstitution","grant_number":"P34607","_id":"fc38323b-9c52-11eb-aca3-ff8afb4a011d"},{"name":"Motile active matter models of migrating cells and chiral filaments","grant_number":"26360","_id":"34d75525-11ca-11ed-8bc3-89b6307fee9d"}]},{"file_date_updated":"2024-01-30T14:28:30Z","department":[{"_id":"JoDa"},{"_id":"EdHa"},{"_id":"MaLo"},{"_id":"GradSch"}],"ddc":["530"],"date_updated":"2024-02-21T12:19:08Z","status":"public","type":"journal_article","article_type":"original","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"_id":"13314","related_material":{"record":[{"relation":"research_data","id":"13116","status":"public"}]},"volume":19,"ec_funded":1,"file":[{"relation":"main_file","access_level":"open_access","content_type":"application/pdf","success":1,"file_id":"14916","checksum":"bc7673ca07d37309013a86166577b2f7","creator":"dernst","file_size":22471673,"date_updated":"2024-01-30T14:28:30Z","file_name":"2023_NaturePhysics_Dunajova.pdf","date_created":"2024-01-30T14:28:30Z"}],"language":[{"iso":"eng"}],"publication_identifier":{"eissn":["1745-2481"],"issn":["1745-2473"]},"publication_status":"published","month":"12","intvolume":" 19","scopus_import":"1","oa_version":"Published Version","pmid":1,"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"abstract":[{"lang":"eng","text":"The emergence of large-scale order in self-organized systems relies on local interactions between individual components. During bacterial cell division, FtsZ—a prokaryotic homologue of the eukaryotic protein tubulin—polymerizes into treadmilling filaments that further organize into a cytoskeletal ring. In vitro, FtsZ filaments can form dynamic chiral assemblies. However, how the active and passive properties of individual filaments relate to these large-scale self-organized structures remains poorly understood. Here we connect single-filament properties with the mesoscopic scale by combining minimal active matter simulations and biochemical reconstitution experiments. We show that the density and flexibility of active chiral filaments define their global order. At intermediate densities, curved, flexible filaments organize into chiral rings and polar bands. An effectively nematic organization dominates for high densities and for straight, mutant filaments with increased rigidity. Our predicted phase diagram quantitatively captures these features, demonstrating how the flexibility, density and chirality of the active filaments affect their collective behaviour. Our findings shed light on the fundamental properties of active chiral matter and explain how treadmilling FtsZ filaments organize during bacterial cell division."}],"title":"Chiral and nematic phases of flexible active filaments","author":[{"id":"4B39F286-F248-11E8-B48F-1D18A9856A87","first_name":"Zuzana","full_name":"Dunajova, Zuzana","last_name":"Dunajova"},{"first_name":"Batirtze","id":"299FE892-F248-11E8-B48F-1D18A9856A87","full_name":"Prats Mateu, Batirtze","last_name":"Prats Mateu"},{"last_name":"Radler","orcid":"0000-0001-9198-2182 ","full_name":"Radler, Philipp","first_name":"Philipp","id":"40136C2A-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Keesiang","full_name":"Lim, Keesiang","last_name":"Lim"},{"last_name":"Brandis","full_name":"Brandis, Dörte","first_name":"Dörte","id":"21d64d35-f128-11eb-9611-b8bcca7a12fd"},{"first_name":"Philipp","id":"39BDC62C-F248-11E8-B48F-1D18A9856A87","last_name":"Velicky","full_name":"Velicky, Philipp","orcid":"0000-0002-2340-7431"},{"first_name":"Johann G","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","last_name":"Danzl","orcid":"0000-0001-8559-3973","full_name":"Danzl, Johann G"},{"full_name":"Wong, Richard W.","last_name":"Wong","first_name":"Richard W."},{"first_name":"Jens","last_name":"Elgeti","full_name":"Elgeti, Jens"},{"last_name":"Hannezo","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"},{"orcid":"0000-0001-7309-9724","full_name":"Loose, Martin","last_name":"Loose","first_name":"Martin","id":"462D4284-F248-11E8-B48F-1D18A9856A87"}],"article_processing_charge":"Yes (in subscription journal)","external_id":{"pmid":["38075437"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"mla":"Dunajova, Zuzana, et al. “Chiral and Nematic Phases of Flexible Active Filaments.” Nature Physics, vol. 19, Springer Nature, 2023, pp. 1916–26, doi:10.1038/s41567-023-02218-w.","ama":"Dunajova Z, Prats Mateu B, Radler P, et al. Chiral and nematic phases of flexible active filaments. Nature Physics. 2023;19:1916-1926. doi:10.1038/s41567-023-02218-w","apa":"Dunajova, Z., Prats Mateu, B., Radler, P., Lim, K., Brandis, D., Velicky, P., … Loose, M. (2023). Chiral and nematic phases of flexible active filaments. Nature Physics. Springer Nature. https://doi.org/10.1038/s41567-023-02218-w","short":"Z. Dunajova, B. Prats Mateu, P. Radler, K. Lim, D. Brandis, P. Velicky, J.G. Danzl, R.W. Wong, J. Elgeti, E.B. Hannezo, M. Loose, Nature Physics 19 (2023) 1916–1926.","ieee":"Z. Dunajova et al., “Chiral and nematic phases of flexible active filaments,” Nature Physics, vol. 19. Springer Nature, pp. 1916–1926, 2023.","chicago":"Dunajova, Zuzana, Batirtze Prats Mateu, Philipp Radler, Keesiang Lim, Dörte Brandis, Philipp Velicky, Johann G Danzl, et al. “Chiral and Nematic Phases of Flexible Active Filaments.” Nature Physics. Springer Nature, 2023. https://doi.org/10.1038/s41567-023-02218-w.","ista":"Dunajova Z, Prats Mateu B, Radler P, Lim K, Brandis D, Velicky P, Danzl JG, Wong RW, Elgeti J, Hannezo EB, Loose M. 2023. Chiral and nematic phases of flexible active filaments. Nature Physics. 19, 1916–1926."},"project":[{"call_identifier":"H2020","_id":"2595697A-B435-11E9-9278-68D0E5697425","grant_number":"679239","name":"Self-Organization of the Bacterial Cell"},{"grant_number":"P34607","name":"Understanding bacterial cell division by in vitro\r\nreconstitution","_id":"fc38323b-9c52-11eb-aca3-ff8afb4a011d"},{"_id":"34d75525-11ca-11ed-8bc3-89b6307fee9d","grant_number":"26360","name":"Motile active matter models of migrating cells and chiral filaments"}],"doi":"10.1038/s41567-023-02218-w","date_published":"2023-12-01T00:00:00Z","date_created":"2023-07-27T14:44:45Z","page":"1916-1926","day":"01","publication":"Nature Physics","has_accepted_license":"1","year":"2023","quality_controlled":"1","publisher":"Springer Nature","oa":1,"acknowledgement":"This work was supported by the European Research Council through grant ERC 2015-StG-679239 and by the Austrian Science Fund (FWF) StandAlone P34607 to M.L., B. P.M. was also supported by the Kanazawa University WPI- NanoLSI Bio-SPM collaborative research program. Z.D. has received funding from Doctoral Programme of the Austrian Academy of Sciences (OeAW): Grant agreement 26360. We thank Jan Brugues (MPI CBG, Dresden, Germany), Andela Saric (ISTA, Klosterneuburg, Austria), Daniel Pearce (Uni Geneva, Switzerland) for valuable scientific input and comments on the manuscript. We are also thankful for the support by the Scientific Service Units (SSU) of IST Austria through resources provided by the Imaging and Optics Facility (IOF) and the Lab Support Facility (LSF)."},{"_id":"9794","status":"public","article_type":"original","type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"ddc":["570"],"date_updated":"2023-08-02T06:53:07Z","file_date_updated":"2022-07-25T07:11:32Z","department":[{"_id":"SiHi"},{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"EM-Fac"},{"_id":"Bio"},{"_id":"MiSi"}],"oa_version":"Published Version","abstract":[{"lang":"eng","text":"Lymph nodes (LNs) comprise two main structural elements: fibroblastic reticular cells that form dedicated niches for immune cell interaction and capsular fibroblasts that build a shell around the organ. Immunological challenge causes LNs to increase more than tenfold in size within a few days. Here, we characterized the biomechanics of LN swelling on the cellular and organ scale. We identified lymphocyte trapping by influx and proliferation as drivers of an outward pressure force, causing fibroblastic reticular cells of the T-zone (TRCs) and their associated conduits to stretch. After an initial phase of relaxation, TRCs sensed the resulting strain through cell matrix adhesions, which coordinated local growth and remodeling of the stromal network. While the expanded TRC network readopted its typical configuration, a massive fibrotic reaction of the organ capsule set in and countered further organ expansion. Thus, different fibroblast populations mechanically control LN swelling in a multitier fashion."}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"PreCl"},{"_id":"LifeSc"}],"month":"07","intvolume":" 23","scopus_import":"1","file":[{"checksum":"628e7b49809f22c75b428842efe70c68","file_id":"11642","success":1,"access_level":"open_access","relation":"main_file","content_type":"application/pdf","date_created":"2022-07-25T07:11:32Z","file_name":"2022_NatureImmunology_Assen.pdf","creator":"dernst","date_updated":"2022-07-25T07:11:32Z","file_size":11475325}],"language":[{"iso":"eng"}],"publication_identifier":{"eissn":["1529-2916"],"issn":["1529-2908"]},"publication_status":"published","volume":23,"ec_funded":1,"project":[{"call_identifier":"H2020","_id":"25FE9508-B435-11E9-9278-68D0E5697425","name":"Cellular navigation along spatial gradients","grant_number":"724373"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"ama":"Assen FP, Abe J, Hons M, et al. Multitier mechanics control stromal adaptations in swelling lymph nodes. Nature Immunology. 2022;23:1246-1255. doi:10.1038/s41590-022-01257-4","apa":"Assen, F. P., Abe, J., Hons, M., Hauschild, R., Shamipour, S., Kaufmann, W., … Sixt, M. K. (2022). Multitier mechanics control stromal adaptations in swelling lymph nodes. Nature Immunology. Springer Nature. https://doi.org/10.1038/s41590-022-01257-4","short":"F.P. Assen, J. Abe, M. Hons, R. Hauschild, S. Shamipour, W. Kaufmann, T. Costanzo, G. Krens, M. Brown, B. Ludewig, S. Hippenmeyer, C.-P.J. Heisenberg, W. Weninger, E.B. Hannezo, S.A. Luther, J.V. Stein, M.K. Sixt, Nature Immunology 23 (2022) 1246–1255.","ieee":"F. P. Assen et al., “Multitier mechanics control stromal adaptations in swelling lymph nodes,” Nature Immunology, vol. 23. Springer Nature, pp. 1246–1255, 2022.","mla":"Assen, Frank P., et al. “Multitier Mechanics Control Stromal Adaptations in Swelling Lymph Nodes.” Nature Immunology, vol. 23, Springer Nature, 2022, pp. 1246–55, doi:10.1038/s41590-022-01257-4.","ista":"Assen FP, Abe J, Hons M, Hauschild R, Shamipour S, Kaufmann W, Costanzo T, Krens G, Brown M, Ludewig B, Hippenmeyer S, Heisenberg C-PJ, Weninger W, Hannezo EB, Luther SA, Stein JV, Sixt MK. 2022. Multitier mechanics control stromal adaptations in swelling lymph nodes. Nature Immunology. 23, 1246–1255.","chicago":"Assen, Frank P, Jun Abe, Miroslav Hons, Robert Hauschild, Shayan Shamipour, Walter Kaufmann, Tommaso Costanzo, et al. “Multitier Mechanics Control Stromal Adaptations in Swelling Lymph Nodes.” Nature Immunology. Springer Nature, 2022. https://doi.org/10.1038/s41590-022-01257-4."},"title":"Multitier mechanics control stromal adaptations in swelling lymph nodes","author":[{"last_name":"Assen","full_name":"Assen, Frank P","orcid":"0000-0003-3470-6119","first_name":"Frank P","id":"3A8E7F24-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Abe","full_name":"Abe, Jun","first_name":"Jun"},{"orcid":"0000-0002-6625-3348","full_name":"Hons, Miroslav","last_name":"Hons","first_name":"Miroslav","id":"4167FE56-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert","last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert"},{"full_name":"Shamipour, Shayan","last_name":"Shamipour","id":"40B34FE2-F248-11E8-B48F-1D18A9856A87","first_name":"Shayan"},{"id":"3F99E422-F248-11E8-B48F-1D18A9856A87","first_name":"Walter","full_name":"Kaufmann, Walter","orcid":"0000-0001-9735-5315","last_name":"Kaufmann"},{"orcid":"0000-0001-9732-3815","full_name":"Costanzo, Tommaso","last_name":"Costanzo","first_name":"Tommaso","id":"D93824F4-D9BA-11E9-BB12-F207E6697425"},{"full_name":"Krens, Gabriel","orcid":"0000-0003-4761-5996","last_name":"Krens","first_name":"Gabriel","id":"2B819732-F248-11E8-B48F-1D18A9856A87"},{"id":"3DAB9AFC-F248-11E8-B48F-1D18A9856A87","first_name":"Markus","full_name":"Brown, Markus","last_name":"Brown"},{"first_name":"Burkhard","last_name":"Ludewig","full_name":"Ludewig, Burkhard"},{"id":"37B36620-F248-11E8-B48F-1D18A9856A87","first_name":"Simon","last_name":"Hippenmeyer","full_name":"Hippenmeyer, Simon","orcid":"0000-0003-2279-1061"},{"last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Wolfgang","last_name":"Weninger","full_name":"Weninger, Wolfgang"},{"last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"},{"first_name":"Sanjiv A.","full_name":"Luther, Sanjiv A.","last_name":"Luther"},{"first_name":"Jens V.","last_name":"Stein","full_name":"Stein, Jens V."},{"last_name":"Sixt","full_name":"Sixt, Michael K","orcid":"0000-0002-4561-241X","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"}],"external_id":{"isi":["000822975900002"]},"article_processing_charge":"No","acknowledgement":"This research was supported by the Scientific Service Units of IST Austria through resources provided by the Imaging and Optics, Electron Microscopy, Preclinical and Life Science Facilities. We thank C. Moussion for providing anti-PNAd antibody and D. Critchley for Talin1-floxed mice, and E. Papusheva for providing a custom 3D channel alignment script. This work was supported by a European Research Council grant ERC-CoG-72437 to M.S. M.H. was supported by Czech Sciencundation GACR 20-24603Y and Charles University PRIMUS/20/MED/013.","publisher":"Springer Nature","quality_controlled":"1","oa":1,"day":"11","publication":"Nature Immunology","isi":1,"has_accepted_license":"1","year":"2022","doi":"10.1038/s41590-022-01257-4","date_published":"2022-07-11T00:00:00Z","date_created":"2021-08-06T09:09:11Z","page":"1246-1255"},{"abstract":[{"text":"Although rigidity and jamming transitions have been widely studied in physics and material science, their importance in a number of biological processes, including embryo development, tissue homeostasis, wound healing, and disease progression, has only begun to be recognized in the past few years. The hypothesis that biological systems can undergo rigidity/jamming transitions is attractive, as it would allow these systems to change their material properties rapidly and strongly. However, whether such transitions indeed occur in biological systems, how they are being regulated, and what their physiological relevance might be, is still being debated. Here, we review theoretical and experimental advances from the past few years, focusing on the regulation and role of potential tissue rigidity transitions in different biological processes.","lang":"eng"}],"oa_version":"None","pmid":1,"scopus_import":"1","month":"05","intvolume":" 32","publication_identifier":{"issn":["0962-8924"],"eissn":["1879-3088"]},"publication_status":"published","language":[{"iso":"eng"}],"volume":32,"issue":"5","_id":"10705","article_type":"original","type":"journal_article","status":"public","date_updated":"2023-08-02T14:03:53Z","department":[{"_id":"EdHa"},{"_id":"CaHe"}],"acknowledgement":"We thank present and former members of the Heisenberg and Hannezo groups, in particular Bernat Corominas-Murtra and Nicoletta Petridou, for helpful discussions, and Claudia Flandoli for the artwork. We apologize for not being able to cite a number of highly relevant studies, to stay within the maximum allowed number of citations.","publisher":"Cell Press","quality_controlled":"1","isi":1,"year":"2022","day":"01","publication":"Trends in Cell Biology","page":"P433-444","doi":"10.1016/j.tcb.2021.12.006","date_published":"2022-05-01T00:00:00Z","date_created":"2022-01-30T23:01:34Z","citation":{"mla":"Hannezo, Edouard B., and Carl-Philipp J. Heisenberg. “Rigidity Transitions in Development and Disease.” Trends in Cell Biology, vol. 32, no. 5, Cell Press, 2022, pp. P433-444, doi:10.1016/j.tcb.2021.12.006.","ama":"Hannezo EB, Heisenberg C-PJ. Rigidity transitions in development and disease. Trends in Cell Biology. 2022;32(5):P433-444. doi:10.1016/j.tcb.2021.12.006","apa":"Hannezo, E. B., & Heisenberg, C.-P. J. (2022). Rigidity transitions in development and disease. Trends in Cell Biology. Cell Press. https://doi.org/10.1016/j.tcb.2021.12.006","short":"E.B. Hannezo, C.-P.J. Heisenberg, Trends in Cell Biology 32 (2022) P433-444.","ieee":"E. B. Hannezo and C.-P. J. Heisenberg, “Rigidity transitions in development and disease,” Trends in Cell Biology, vol. 32, no. 5. Cell Press, pp. P433-444, 2022.","chicago":"Hannezo, Edouard B, and Carl-Philipp J Heisenberg. “Rigidity Transitions in Development and Disease.” Trends in Cell Biology. Cell Press, 2022. https://doi.org/10.1016/j.tcb.2021.12.006.","ista":"Hannezo EB, Heisenberg C-PJ. 2022. Rigidity transitions in development and disease. Trends in Cell Biology. 32(5), P433-444."},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","author":[{"first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo"},{"orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"external_id":{"isi":["000795773900009"],"pmid":["35058104"]},"article_processing_charge":"No","title":"Rigidity transitions in development and disease"},{"publisher":"Cell Press","quality_controlled":"1","oa":1,"acknowledgement":"We are grateful to H. Niwa for Dox regulatable PB vector; G. Charras for EzrinT567D cDNA; K. Jones for tdTomato ESCs, R26-Confetti ESCs, and laboratory assistance; M. Kinoshita for pPB-CAG-H2B-BFP plasmid; P. Humphreys and D. Clements for imaging support; G. Chu, P. Attlesey, and staff for animal husbandry; S. Pallett for laboratory assistance; C. Mulas for critical feedback on the project; T. Boroviak for single-cell RNA-seq; the EMBL Genomics Core Facility for sequencing; and M. Merkel for developing and sharing the original version of the 3D Voronoi code. This work was financially supported by BBSRC ( BB/Moo4023/1 and BB/T007044/1 to K.J.C. and J.N., Alert16 grant BB/R000042 to E.K.P.), Leverhulme Trust ( RPG-2014-080 to K.J.C. and J.N.), European Research Council ( 772798 -CellFateTech to K.J.C., 311637 -MorphoCorDiv and 820188 -NanoMechShape to E.K.P., Starting Grant 851288 to E.H., and 772426 -MeChemGui to K.F.), the Isaac Newton Trust (to E.K.P.), Medical Research Council UK (MRC program award MC_UU_00012/5 to E.K.P.), the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement no. 641639 ( ITN Biopol , H.D.B. and E.K.P.), the Alexander von Humboldt Foundation (Alexander von Humboldt Professorship to K.F.), EMBO ALTF 522-2021 (to P.S.), Centre for Trophoblast Research (Next Generation fellowship to S.A.), and JSPS Overseas Research Fellowships (to A.Y.). The Wellcome-MRC Cambridge Stem Cell Institute receives core funding from Wellcome Trust ( 203151/Z/16/Z ) and MRC ( MC_PC_17230 ). For the purpose of open access, the author has applied a CC BY public copyright licence to any Author Accepted Manuscript version arising from this submission.","date_published":"2022-02-22T00:00:00Z","doi":"10.1016/j.cell.2022.01.022","date_created":"2022-03-06T23:01:52Z","page":"777-793.e20","day":"22","publication":"Cell","isi":1,"has_accepted_license":"1","year":"2022","project":[{"name":"Design Principles of Branching Morphogenesis","grant_number":"851288","_id":"05943252-7A3F-11EA-A408-12923DDC885E","call_identifier":"H2020"}],"title":"Cell surface fluctuations regulate early embryonic lineage sorting","author":[{"first_name":"Ayaka","full_name":"Yanagida, Ayaka","last_name":"Yanagida"},{"first_name":"Elena","last_name":"Corujo-Simon","full_name":"Corujo-Simon, Elena"},{"first_name":"Christopher K.","full_name":"Revell, Christopher K.","last_name":"Revell"},{"last_name":"Sahu","full_name":"Sahu, Preeti","first_name":"Preeti","id":"55BA52EE-A185-11EA-88FD-18AD3DDC885E"},{"last_name":"Stirparo","full_name":"Stirparo, Giuliano G.","first_name":"Giuliano G."},{"last_name":"Aspalter","full_name":"Aspalter, Irene M.","first_name":"Irene M."},{"first_name":"Alex K.","last_name":"Winkel","full_name":"Winkel, Alex K."},{"first_name":"Ruby","last_name":"Peters","full_name":"Peters, Ruby"},{"last_name":"De Belly","full_name":"De Belly, Henry","first_name":"Henry"},{"first_name":"Davide A.D.","last_name":"Cassani","full_name":"Cassani, Davide A.D."},{"full_name":"Achouri, Sarra","last_name":"Achouri","first_name":"Sarra"},{"first_name":"Raphael","last_name":"Blumenfeld","full_name":"Blumenfeld, Raphael"},{"full_name":"Franze, Kristian","last_name":"Franze","first_name":"Kristian"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","last_name":"Hannezo"},{"first_name":"Ewa K.","full_name":"Paluch, Ewa K.","last_name":"Paluch"},{"first_name":"Jennifer","full_name":"Nichols, Jennifer","last_name":"Nichols"},{"last_name":"Chalut","full_name":"Chalut, Kevin J.","first_name":"Kevin J."}],"external_id":{"isi":["000796293700007"],"pmid":["35196500"]},"article_processing_charge":"No","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"ista":"Yanagida A, Corujo-Simon E, Revell CK, Sahu P, Stirparo GG, Aspalter IM, Winkel AK, Peters R, De Belly H, Cassani DAD, Achouri S, Blumenfeld R, Franze K, Hannezo EB, Paluch EK, Nichols J, Chalut KJ. 2022. Cell surface fluctuations regulate early embryonic lineage sorting. Cell. 185(5), 777–793.e20.","chicago":"Yanagida, Ayaka, Elena Corujo-Simon, Christopher K. Revell, Preeti Sahu, Giuliano G. Stirparo, Irene M. Aspalter, Alex K. Winkel, et al. “Cell Surface Fluctuations Regulate Early Embryonic Lineage Sorting.” Cell. Cell Press, 2022. https://doi.org/10.1016/j.cell.2022.01.022.","apa":"Yanagida, A., Corujo-Simon, E., Revell, C. K., Sahu, P., Stirparo, G. G., Aspalter, I. M., … Chalut, K. J. (2022). Cell surface fluctuations regulate early embryonic lineage sorting. Cell. Cell Press. https://doi.org/10.1016/j.cell.2022.01.022","ama":"Yanagida A, Corujo-Simon E, Revell CK, et al. Cell surface fluctuations regulate early embryonic lineage sorting. Cell. 2022;185(5):777-793.e20. doi:10.1016/j.cell.2022.01.022","short":"A. Yanagida, E. Corujo-Simon, C.K. Revell, P. Sahu, G.G. Stirparo, I.M. Aspalter, A.K. Winkel, R. Peters, H. De Belly, D.A.D. Cassani, S. Achouri, R. Blumenfeld, K. Franze, E.B. Hannezo, E.K. Paluch, J. Nichols, K.J. Chalut, Cell 185 (2022) 777–793.e20.","ieee":"A. Yanagida et al., “Cell surface fluctuations regulate early embryonic lineage sorting,” Cell, vol. 185, no. 5. Cell Press, p. 777–793.e20, 2022.","mla":"Yanagida, Ayaka, et al. “Cell Surface Fluctuations Regulate Early Embryonic Lineage Sorting.” Cell, vol. 185, no. 5, Cell Press, 2022, p. 777–793.e20, doi:10.1016/j.cell.2022.01.022."},"month":"02","intvolume":" 185","scopus_import":"1","oa_version":"Published Version","pmid":1,"abstract":[{"lang":"eng","text":"In development, lineage segregation is coordinated in time and space. An important example is the mammalian inner cell mass, in which the primitive endoderm (PrE, founder of the yolk sac) physically segregates from the epiblast (EPI, founder of the fetus). While the molecular requirements have been well studied, the physical mechanisms determining spatial segregation between EPI and PrE remain elusive. Here, we investigate the mechanical basis of EPI and PrE sorting. We find that rather than the differences in static cell surface mechanical parameters as in classical sorting models, it is the differences in surface fluctuations that robustly ensure physical lineage sorting. These differential surface fluctuations systematically correlate with differential cellular fluidity, which we propose together constitute a non-equilibrium sorting mechanism for EPI and PrE lineages. By combining experiments and modeling, we identify cell surface dynamics as a key factor orchestrating the correct spatial segregation of the founder embryonic lineages."}],"volume":185,"issue":"5","ec_funded":1,"file":[{"success":1,"file_id":"10831","checksum":"ae305060e8031297771b89dae9e36a29","content_type":"application/pdf","relation":"main_file","access_level":"open_access","file_name":"2022_Cell_Yanagida.pdf","date_created":"2022-03-07T07:55:23Z","file_size":8478995,"date_updated":"2022-03-07T07:55:23Z","creator":"dernst"}],"language":[{"iso":"eng"}],"publication_identifier":{"eissn":["10974172"],"issn":["00928674"]},"publication_status":"published","status":"public","type":"journal_article","article_type":"original","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"_id":"10825","file_date_updated":"2022-03-07T07:55:23Z","department":[{"_id":"EdHa"}],"ddc":["570"],"date_updated":"2023-08-02T14:43:50Z"},{"project":[{"grant_number":"ALTF 850-2017","name":"Coordination of mesendoderm cell fate specification and internalization during zebrafish gastrulation","_id":"26520D1E-B435-11E9-9278-68D0E5697425"},{"_id":"26520D1E-B435-11E9-9278-68D0E5697425","grant_number":"ALTF 850-2017","name":"Coordination of mesendoderm cell fate specification and internalization during zebrafish gastrulation"},{"name":"Design Principles of Branching Morphogenesis","grant_number":"851288","_id":"05943252-7A3F-11EA-A408-12923DDC885E","call_identifier":"H2020"},{"grant_number":"742573","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","_id":"260F1432-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"ista":"Nunes Pinheiro DC, Kardos R, Hannezo EB, Heisenberg C-PJ. 2022. Morphogen gradient orchestrates pattern-preserving tissue morphogenesis via motility-driven unjamming. Nature Physics. 18(12), 1482–1493.","chicago":"Nunes Pinheiro, Diana C, Roland Kardos, Edouard B Hannezo, and Carl-Philipp J Heisenberg. “Morphogen Gradient Orchestrates Pattern-Preserving Tissue Morphogenesis via Motility-Driven Unjamming.” Nature Physics. Springer Nature, 2022. https://doi.org/10.1038/s41567-022-01787-6.","ieee":"D. C. Nunes Pinheiro, R. Kardos, E. B. Hannezo, and C.-P. J. Heisenberg, “Morphogen gradient orchestrates pattern-preserving tissue morphogenesis via motility-driven unjamming,” Nature Physics, vol. 18, no. 12. Springer Nature, pp. 1482–1493, 2022.","short":"D.C. Nunes Pinheiro, R. Kardos, E.B. Hannezo, C.-P.J. Heisenberg, Nature Physics 18 (2022) 1482–1493.","apa":"Nunes Pinheiro, D. C., Kardos, R., Hannezo, E. B., & Heisenberg, C.-P. J. (2022). Morphogen gradient orchestrates pattern-preserving tissue morphogenesis via motility-driven unjamming. Nature Physics. Springer Nature. https://doi.org/10.1038/s41567-022-01787-6","ama":"Nunes Pinheiro DC, Kardos R, Hannezo EB, Heisenberg C-PJ. Morphogen gradient orchestrates pattern-preserving tissue morphogenesis via motility-driven unjamming. Nature Physics. 2022;18(12):1482-1493. doi:10.1038/s41567-022-01787-6","mla":"Nunes Pinheiro, Diana C., et al. “Morphogen Gradient Orchestrates Pattern-Preserving Tissue Morphogenesis via Motility-Driven Unjamming.” Nature Physics, vol. 18, no. 12, Springer Nature, 2022, pp. 1482–93, doi:10.1038/s41567-022-01787-6."},"title":"Morphogen gradient orchestrates pattern-preserving tissue morphogenesis via motility-driven unjamming","external_id":{"isi":["000871319900002"]},"article_processing_charge":"No","author":[{"id":"2E839F16-F248-11E8-B48F-1D18A9856A87","first_name":"Diana C","full_name":"Nunes Pinheiro, Diana C","orcid":"0000-0003-4333-7503","last_name":"Nunes Pinheiro"},{"last_name":"Kardos","full_name":"Kardos, Roland","first_name":"Roland","id":"4039350E-F248-11E8-B48F-1D18A9856A87"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo"},{"orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J"}],"acknowledgement":"We thank K. Sampath, A. Pauli and Y. Bellaїche for feedback on the manuscript. We also thank the members of the Heisenberg group, in particular A. Schauer and F. Nur Arslan, for help, technical advice and discussions, and the Bioimaging and Life Science facilities at IST\r\nAustria for continuous support. We thank C. Flandoli for the artwork in the figures. This work was supported by postdoctoral fellowships from EMBO (LTF-850-2017) and HFSP (LT000429/2018-L2) to D.P. and the European Union (European Research Council starting grant 851288 to É.H. and European Research Council advanced grant 742573 to C.-P.H.).","oa":1,"quality_controlled":"1","publisher":"Springer Nature","publication":"Nature Physics","day":"01","year":"2022","has_accepted_license":"1","isi":1,"date_created":"2023-01-16T09:45:19Z","doi":"10.1038/s41567-022-01787-6","date_published":"2022-12-01T00:00:00Z","page":"1482-1493","_id":"12209","keyword":["General Physics and Astronomy"],"status":"public","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_type":"original","type":"journal_article","ddc":["570"],"date_updated":"2023-08-04T09:15:58Z","department":[{"_id":"CaHe"},{"_id":"EdHa"}],"file_date_updated":"2023-01-27T07:32:01Z","oa_version":"Published Version","abstract":[{"lang":"eng","text":"Embryo development requires biochemical signalling to generate patterns of cell fates and active mechanical forces to drive tissue shape changes. However, how these processes are coordinated, and how tissue patterning is preserved despite the cellular flows occurring during morphogenesis, remains poorly understood. Gastrulation is a crucial embryonic stage that involves both patterning and internalization of the mesendoderm germ layer tissue. Here we show that, in zebrafish embryos, a gradient in Nodal signalling orchestrates pattern-preserving internalization movements by triggering a motility-driven unjamming transition. In addition to its role as a morphogen determining embryo patterning, graded Nodal signalling mechanically subdivides the mesendoderm into a small fraction of highly protrusive leader cells, able to autonomously internalize via local unjamming, and less protrusive followers, which need to be pulled inwards by the leaders. The Nodal gradient further enforces a code of preferential adhesion coupling leaders to their immediate followers, resulting in a collective and ordered mode of internalization that preserves mesendoderm patterning. Integrating this dual mechanical role of Nodal signalling into minimal active particle simulations quantitatively predicts both physiological and experimentally perturbed internalization movements. This provides a quantitative framework for how a morphogen-encoded unjamming transition can bidirectionally couple tissue mechanics with patterning during complex three-dimensional morphogenesis."}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"intvolume":" 18","month":"12","scopus_import":"1","language":[{"iso":"eng"}],"file":[{"file_id":"12412","checksum":"c86a8e8d80d1bfc46d56a01e88a2526a","success":1,"content_type":"application/pdf","access_level":"open_access","relation":"main_file","date_created":"2023-01-27T07:32:01Z","file_name":"2022_NaturePhysics_Pinheiro.pdf","date_updated":"2023-01-27T07:32:01Z","file_size":36703569,"creator":"dernst"}],"publication_status":"published","publication_identifier":{"issn":["1745-2473"],"eissn":["1745-2481"]},"ec_funded":1,"issue":"12","volume":18},{"project":[{"_id":"05943252-7A3F-11EA-A408-12923DDC885E","call_identifier":"H2020","name":"Design Principles of Branching Morphogenesis","grant_number":"851288"}],"article_number":"5219","title":"Spatiotemporal dynamics of self-organized branching in pancreas-derived organoids","author":[{"last_name":"Randriamanantsoa","full_name":"Randriamanantsoa, S.","first_name":"S."},{"first_name":"A.","full_name":"Papargyriou, A.","last_name":"Papargyriou"},{"last_name":"Maurer","full_name":"Maurer, H. C.","first_name":"H. C."},{"first_name":"K.","full_name":"Peschke, K.","last_name":"Peschke"},{"first_name":"M.","last_name":"Schuster","full_name":"Schuster, M."},{"first_name":"G.","last_name":"Zecchin","full_name":"Zecchin, G."},{"full_name":"Steiger, K.","last_name":"Steiger","first_name":"K."},{"first_name":"R.","full_name":"Öllinger, R.","last_name":"Öllinger"},{"full_name":"Saur, D.","last_name":"Saur","first_name":"D."},{"first_name":"C.","last_name":"Scheel","full_name":"Scheel, C."},{"first_name":"R.","last_name":"Rad","full_name":"Rad, R."},{"last_name":"Hannezo","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Reichert","full_name":"Reichert, M.","first_name":"M."},{"full_name":"Bausch, A. R.","last_name":"Bausch","first_name":"A. R."}],"article_processing_charge":"No","external_id":{"isi":["000850348400025"]},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"chicago":"Randriamanantsoa, S., A. Papargyriou, H. C. Maurer, K. Peschke, M. Schuster, G. Zecchin, K. Steiger, et al. “Spatiotemporal Dynamics of Self-Organized Branching in Pancreas-Derived Organoids.” Nature Communications. Springer Nature, 2022. https://doi.org/10.1038/s41467-022-32806-y.","ista":"Randriamanantsoa S, Papargyriou A, Maurer HC, Peschke K, Schuster M, Zecchin G, Steiger K, Öllinger R, Saur D, Scheel C, Rad R, Hannezo EB, Reichert M, Bausch AR. 2022. Spatiotemporal dynamics of self-organized branching in pancreas-derived organoids. Nature Communications. 13, 5219.","mla":"Randriamanantsoa, S., et al. “Spatiotemporal Dynamics of Self-Organized Branching in Pancreas-Derived Organoids.” Nature Communications, vol. 13, 5219, Springer Nature, 2022, doi:10.1038/s41467-022-32806-y.","ieee":"S. Randriamanantsoa et al., “Spatiotemporal dynamics of self-organized branching in pancreas-derived organoids,” Nature Communications, vol. 13. Springer Nature, 2022.","short":"S. Randriamanantsoa, A. Papargyriou, H.C. Maurer, K. Peschke, M. Schuster, G. Zecchin, K. Steiger, R. Öllinger, D. Saur, C. Scheel, R. Rad, E.B. Hannezo, M. Reichert, A.R. Bausch, Nature Communications 13 (2022).","ama":"Randriamanantsoa S, Papargyriou A, Maurer HC, et al. Spatiotemporal dynamics of self-organized branching in pancreas-derived organoids. Nature Communications. 2022;13. doi:10.1038/s41467-022-32806-y","apa":"Randriamanantsoa, S., Papargyriou, A., Maurer, H. C., Peschke, K., Schuster, M., Zecchin, G., … Bausch, A. R. (2022). Spatiotemporal dynamics of self-organized branching in pancreas-derived organoids. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-022-32806-y"},"quality_controlled":"1","publisher":"Springer Nature","oa":1,"acknowledgement":"A.R.B. acknowledges the financial support of the European Research Council (ERC) through the funding of the grant Principles of Integrin Mechanics and Adhesion (PoINT) and the German Research Foundation (DFG, SFB 1032, project ID 201269156). E.H. was supported by the European Union (European Research Council Starting Grant 851288). D.S., M.R., and R.R. acknowledge the support by the German Research Foundation (DFG, SFB1321 Modeling and Targeting Pancreatic Cancer, Project S01, project ID 329628492). C.S. and M.R. acknowledge the support by the German Research Foundation (DFG, SFB1321 Modeling and Targeting Pancreatic Cancer, Project 12, project ID 329628492). M.R. was supported by the German Research Foundation (DFG RE 3723/4-1). A.P. and M.R. were supported by the German Cancer Aid (Max-Eder Program 111273 and 70114328).\r\nOpen Access funding enabled and organized by Projekt DEAL.","doi":"10.1038/s41467-022-32806-y","date_published":"2022-09-05T00:00:00Z","date_created":"2023-01-16T09:46:53Z","day":"05","publication":"Nature Communications","has_accepted_license":"1","isi":1,"year":"2022","status":"public","keyword":["General Physics and Astronomy","General Biochemistry","Genetics and Molecular Biology","General Chemistry","Multidisciplinary"],"type":"journal_article","article_type":"original","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"_id":"12217","department":[{"_id":"EdHa"}],"file_date_updated":"2023-01-27T08:14:48Z","ddc":["570"],"date_updated":"2023-08-04T09:25:23Z","month":"09","intvolume":" 13","scopus_import":"1","oa_version":"Published Version","abstract":[{"text":"The development dynamics and self-organization of glandular branched epithelia is of utmost importance for our understanding of diverse processes ranging from normal tissue growth to the growth of cancerous tissues. Using single primary murine pancreatic ductal adenocarcinoma (PDAC) cells embedded in a collagen matrix and adapted media supplementation, we generate organoids that self-organize into highly branched structures displaying a seamless lumen connecting terminal end buds, replicating in vivo PDAC architecture. We identify distinct morphogenesis phases, each characterized by a unique pattern of cell invasion, matrix deformation, protein expression, and respective molecular dependencies. We propose a minimal theoretical model of a branching and proliferating tissue, capturing the dynamics of the first phases. Observing the interaction of morphogenesis, mechanical environment and gene expression in vitro sets a benchmark for the understanding of self-organization processes governing complex organoid structure formation processes and branching morphogenesis.","lang":"eng"}],"related_material":{"record":[{"id":"13068","status":"public","relation":"research_data"}]},"volume":13,"ec_funded":1,"file":[{"relation":"main_file","access_level":"open_access","content_type":"application/pdf","success":1,"file_id":"12416","checksum":"295261b5172274fd5b8f85a6a6058828","creator":"dernst","file_size":22645149,"date_updated":"2023-01-27T08:14:48Z","file_name":"2022_NatureCommunications_Randriamanantsoa.pdf","date_created":"2023-01-27T08:14:48Z"}],"language":[{"iso":"eng"}],"publication_identifier":{"issn":["2041-1723"]},"publication_status":"published"},{"language":[{"iso":"eng"}],"file":[{"date_created":"2023-01-30T09:27:49Z","file_name":"2022_ScienceAdvances_Stock.pdf","creator":"dernst","date_updated":"2023-01-30T09:27:49Z","file_size":1636732,"file_id":"12444","checksum":"f59cdb824e5d4221045def81f46f6c65","success":1,"access_level":"open_access","relation":"main_file","content_type":"application/pdf"}],"publication_status":"published","publication_identifier":{"issn":["2375-2548"]},"ec_funded":1,"volume":8,"issue":"37","pmid":1,"oa_version":"Published Version","abstract":[{"lang":"eng","text":"The sculpting of germ layers during gastrulation relies on the coordinated migration of progenitor cells, yet the cues controlling these long-range directed movements remain largely unknown. While directional migration often relies on a chemokine gradient generated from a localized source, we find that zebrafish ventrolateral mesoderm is guided by a self-generated gradient of the initially uniformly expressed and secreted protein Toddler/ELABELA/Apela. We show that the Apelin receptor, which is specifically expressed in mesodermal cells, has a dual role during gastrulation, acting as a scavenger receptor to generate a Toddler gradient, and as a chemokine receptor to sense this guidance cue. Thus, we uncover a single receptor–based self-generated gradient as the enigmatic guidance cue that can robustly steer the directional migration of mesoderm through the complex and continuously changing environment of the gastrulating embryo."}],"intvolume":" 8","month":"09","scopus_import":"1","ddc":["570"],"date_updated":"2023-08-04T09:49:59Z","file_date_updated":"2023-01-30T09:27:49Z","department":[{"_id":"EdHa"}],"_id":"12253","status":"public","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"type":"journal_article","article_type":"original","publication":"Science Advances","day":"14","year":"2022","isi":1,"has_accepted_license":"1","date_created":"2023-01-16T09:57:10Z","date_published":"2022-09-14T00:00:00Z","doi":"10.1126/sciadv.add2488","acknowledgement":"We thank K. Aumayer and the team of the biooptics facility at the Vienna Biocenter, particularly P. Pasierbek and T. Müller, for support with microscopy; K. Panser, C. Pribitzer, and the animal facility personnel for taking care of zebrafish; M. Binner and A. Bandura for help with genotyping; M. Codina Tobias for help with establishing the conditions for the Toddler overexpression compensation experiment; T. Lubiana Alves for sharing the code for scRNA-Seq analyses; the Heisenberg laboratory, particularly D. Pinheiro, for joint laboratory meetings, discussions on the project, and providing the tg(gsc:CAAX-GFP) fish line; the Raz laboratory for providing the Lifeact-GFP plasmid; A. Andersen, A. Schier, C.-P. Heisenberg, and E. Tanaka for comments on the manuscript; and the entire Pauli laboratory, particularly K. Gert and V. Deneke, for valuable discussions and feedback on the manuscript. Funding: Work in A.P.’s laboratory has been supported by the IMP, which receives institutional funding from Boehringer Ingelheim and the Austrian Research Promotion Agency (Headquarter grant FFG-852936), as well as the FWF START program (Y 1031-B28 to A.P.), the Human Frontier Science Program (HFSP) Career Development Award (CDA00066/2015 to A.P.) and Young Investigator Grant (RGY0079/2020 to A.P.), the SFB RNA-Deco (project number F 80 to A.P.), a Whitman Center Fellowship from the Marine Biological Laboratory (to A.P.), and EMBO-YIP funds (to A.P.). This work was supported by the European Union (European Research Council Starting Grant 851288 to E.H.). For the purpose of Open Access, the authors have applied a CC BY public copyright license to any Author Accepted Manuscript (AAM) version arising from this submission.","oa":1,"quality_controlled":"1","publisher":"American Association for the Advancement of Science","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"ista":"Stock J, Kazmar T, Schlumm F, Hannezo EB, Pauli A. 2022. A self-generated Toddler gradient guides mesodermal cell migration. Science Advances. 8(37), eadd2488.","chicago":"Stock, Jessica, Tomas Kazmar, Friederike Schlumm, Edouard B Hannezo, and Andrea Pauli. “A Self-Generated Toddler Gradient Guides Mesodermal Cell Migration.” Science Advances. American Association for the Advancement of Science, 2022. https://doi.org/10.1126/sciadv.add2488.","apa":"Stock, J., Kazmar, T., Schlumm, F., Hannezo, E. B., & Pauli, A. (2022). A self-generated Toddler gradient guides mesodermal cell migration. Science Advances. American Association for the Advancement of Science. https://doi.org/10.1126/sciadv.add2488","ama":"Stock J, Kazmar T, Schlumm F, Hannezo EB, Pauli A. A self-generated Toddler gradient guides mesodermal cell migration. Science Advances. 2022;8(37). doi:10.1126/sciadv.add2488","ieee":"J. Stock, T. Kazmar, F. Schlumm, E. B. Hannezo, and A. Pauli, “A self-generated Toddler gradient guides mesodermal cell migration,” Science Advances, vol. 8, no. 37. American Association for the Advancement of Science, 2022.","short":"J. Stock, T. Kazmar, F. Schlumm, E.B. Hannezo, A. Pauli, Science Advances 8 (2022).","mla":"Stock, Jessica, et al. “A Self-Generated Toddler Gradient Guides Mesodermal Cell Migration.” Science Advances, vol. 8, no. 37, eadd2488, American Association for the Advancement of Science, 2022, doi:10.1126/sciadv.add2488."},"title":"A self-generated Toddler gradient guides mesodermal cell migration","external_id":{"pmid":["36103529"],"isi":["000888875000009"]},"article_processing_charge":"No","author":[{"full_name":"Stock, Jessica","last_name":"Stock","first_name":"Jessica"},{"last_name":"Kazmar","full_name":"Kazmar, Tomas","first_name":"Tomas"},{"full_name":"Schlumm, Friederike","last_name":"Schlumm","first_name":"Friederike"},{"full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"},{"full_name":"Pauli, Andrea","last_name":"Pauli","first_name":"Andrea"}],"article_number":"eadd2488","project":[{"_id":"05943252-7A3F-11EA-A408-12923DDC885E","call_identifier":"H2020","grant_number":"851288","name":"Design Principles of Branching Morphogenesis"}]},{"article_number":"031041","citation":{"ieee":"D. Brückner et al., “Geometry adaptation of protrusion and polarity dynamics in confined cell migration,” Physical Review X, vol. 12, no. 3. American Physical Society, 2022.","short":"D. Brückner, M. Schmitt, A. Fink, G. Ladurner, J. Flommersfeld, N. Arlt, E.B. Hannezo, J.O. Rädler, C.P. Broedersz, Physical Review X 12 (2022).","ama":"Brückner D, Schmitt M, Fink A, et al. Geometry adaptation of protrusion and polarity dynamics in confined cell migration. Physical Review X. 2022;12(3). doi:10.1103/physrevx.12.031041","apa":"Brückner, D., Schmitt, M., Fink, A., Ladurner, G., Flommersfeld, J., Arlt, N., … Broedersz, C. P. (2022). Geometry adaptation of protrusion and polarity dynamics in confined cell migration. Physical Review X. American Physical Society. https://doi.org/10.1103/physrevx.12.031041","mla":"Brückner, David, et al. “Geometry Adaptation of Protrusion and Polarity Dynamics in Confined Cell Migration.” Physical Review X, vol. 12, no. 3, 031041, American Physical Society, 2022, doi:10.1103/physrevx.12.031041.","ista":"Brückner D, Schmitt M, Fink A, Ladurner G, Flommersfeld J, Arlt N, Hannezo EB, Rädler JO, Broedersz CP. 2022. Geometry adaptation of protrusion and polarity dynamics in confined cell migration. Physical Review X. 12(3), 031041.","chicago":"Brückner, David, Matthew Schmitt, Alexandra Fink, Georg Ladurner, Johannes Flommersfeld, Nicolas Arlt, Edouard B Hannezo, Joachim O. Rädler, and Chase P. Broedersz. “Geometry Adaptation of Protrusion and Polarity Dynamics in Confined Cell Migration.” Physical Review X. American Physical Society, 2022. https://doi.org/10.1103/physrevx.12.031041."},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","author":[{"id":"e1e86031-6537-11eb-953a-f7ab92be508d","first_name":"David","last_name":"Brückner","full_name":"Brückner, David","orcid":"0000-0001-7205-2975"},{"full_name":"Schmitt, Matthew","last_name":"Schmitt","first_name":"Matthew"},{"full_name":"Fink, Alexandra","last_name":"Fink","first_name":"Alexandra"},{"full_name":"Ladurner, Georg","last_name":"Ladurner","first_name":"Georg"},{"first_name":"Johannes","last_name":"Flommersfeld","full_name":"Flommersfeld, Johannes"},{"last_name":"Arlt","full_name":"Arlt, Nicolas","first_name":"Nicolas"},{"first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","last_name":"Hannezo"},{"first_name":"Joachim O.","full_name":"Rädler, Joachim O.","last_name":"Rädler"},{"first_name":"Chase P.","full_name":"Broedersz, Chase P.","last_name":"Broedersz"}],"article_processing_charge":"No","external_id":{"isi":["000861534700001"],"arxiv":["2106.01014"]},"title":"Geometry adaptation of protrusion and polarity dynamics in confined cell migration","acknowledgement":"We thank Grzegorz Gradziuk, StevenRiedijk, Janni Harju, and M. R. Schnucki for helpful discussions, and Andriy Goychuk for advice on the image segmentation. This project\r\nwas funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation), Project No. 201269156—SFB 1032 (Projects B01 and B12). D. B. B. is supported by the NOMIS Foundation and in part by a DFG fellowship within the Graduate School of Quantitative Biosciences Munich (QBM), as well as by the Joachim Herz Stiftung.","publisher":"American Physical Society","quality_controlled":"1","oa":1,"has_accepted_license":"1","isi":1,"year":"2022","day":"20","publication":"Physical Review X","doi":"10.1103/physrevx.12.031041","date_published":"2022-09-20T00:00:00Z","date_created":"2023-01-16T10:02:06Z","_id":"12277","type":"journal_article","article_type":"original","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"status":"public","keyword":["General Physics and Astronomy"],"date_updated":"2023-08-04T10:25:49Z","ddc":["530","570"],"file_date_updated":"2023-01-30T11:07:27Z","department":[{"_id":"EdHa"}],"abstract":[{"text":"Cell migration in confining physiological environments relies on the concerted dynamics of several cellular components, including protrusions, adhesions with the environment, and the cell nucleus. However, it remains poorly understood how the dynamic interplay of these components and the cell polarity determine the emergent migration behavior at the cellular scale. Here, we combine data-driven inference with a mechanistic bottom-up approach to develop a model for protrusion and polarity dynamics in confined cell migration, revealing how the cellular dynamics adapt to confining geometries. Specifically, we use experimental data of joint protrusion-nucleus migration trajectories of cells on confining micropatterns to systematically determine a mechanistic model linking the stochastic dynamics of cell polarity, protrusions, and nucleus. This model indicates that the cellular dynamics adapt to confining constrictions through a switch in the polarity dynamics from a negative to a positive self-reinforcing feedback loop. Our model further reveals how this feedback loop leads to stereotypical cycles of protrusion-nucleus dynamics that drive the migration of the cell through constrictions. These cycles are disrupted upon perturbation of cytoskeletal components, indicating that the positive feedback is controlled by cellular migration mechanisms. Our data-driven theoretical approach therefore identifies polarity feedback adaptation as a key mechanism in confined cell migration.","lang":"eng"}],"oa_version":"Published Version","scopus_import":"1","month":"09","intvolume":" 12","publication_identifier":{"issn":["2160-3308"]},"publication_status":"published","file":[{"file_name":"2022_PhysicalReviewX_Brueckner.pdf","date_created":"2023-01-30T11:07:27Z","creator":"dernst","file_size":4686804,"date_updated":"2023-01-30T11:07:27Z","success":1,"file_id":"12458","checksum":"40a8fbc3663bf07b37cb80020974d40d","relation":"main_file","access_level":"open_access","content_type":"application/pdf"}],"language":[{"iso":"eng"}],"issue":"3","volume":12},{"quality_controlled":"1","publisher":"Springer Nature","oa":1,"acknowledgement":"We thank the members of the van Rheenen laboratory for reading the manuscript, and the members of the bioimaging, FACS and animal facility of the NKI for experimental support. We acknowledge the staff at the MedH Flow Cytometry core facility, Karolinska Institutet, and LCI facility/Nikon Center of Excellence, Karolinska Institutet. This work was financially supported by the Netherlands Organization of Scientific Research NWO (Veni grant 863.15.011 to S.I.J.E. and Vici grant 09150182110004 to J.v.R.) and the CancerGenomics.nl (Netherlands Organisation for Scientific Research) program (to J.v.R.) the Doctor Josef Steiner Foundation (to J.v.R). B.D.S. acknowledges funding from the Royal Society E.P. Abraham Research Professorship (RP\\R1\\180165) and the Wellcome Trust (098357/Z/12/Z and 219478/Z/19/Z). B.C.-M. acknowledges the support of the field of excellence ‘Complexity of life in basic research and innovation’ of the University of Graz. O.J.S. and their laboratory acknowledge CRUK core funding to the CRUK Beatson Institute (A17196 and A31287) and CRUK core funding to the Sansom laboratory (A21139). P.K. and their laboratory are supported by grants from the Swedish Research Council (2018-03078), Cancerfonden (190634), Academy of Finland Centre of Excellence (266869, 304591 and 320185) and the Jane and Aatos Erkko Foundation. P.L. has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. 758617). E.H. acknowledges funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. 851288).","doi":"10.1038/s41586-022-04962-0","date_published":"2022-07-13T00:00:00Z","date_created":"2023-01-16T10:01:29Z","page":"548-554","day":"13","publication":"Nature","isi":1,"year":"2022","project":[{"call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E","grant_number":"851288","name":"Design Principles of Branching Morphogenesis"}],"title":"Retrograde movements determine effective stem cell numbers in the intestine","author":[{"first_name":"Maria","last_name":"Azkanaz","full_name":"Azkanaz, Maria"},{"last_name":"Corominas-Murtra","full_name":"Corominas-Murtra, Bernat","orcid":"0000-0001-9806-5643","id":"43BE2298-F248-11E8-B48F-1D18A9856A87","first_name":"Bernat"},{"first_name":"Saskia I. J.","full_name":"Ellenbroek, Saskia I. J.","last_name":"Ellenbroek"},{"last_name":"Bruens","full_name":"Bruens, Lotte","first_name":"Lotte"},{"last_name":"Webb","full_name":"Webb, Anna T.","first_name":"Anna T."},{"first_name":"Dimitrios","full_name":"Laskaris, Dimitrios","last_name":"Laskaris"},{"first_name":"Koen C.","full_name":"Oost, Koen C.","last_name":"Oost"},{"full_name":"Lafirenze, Simona J. A.","last_name":"Lafirenze","first_name":"Simona J. A."},{"full_name":"Annusver, Karl","last_name":"Annusver","first_name":"Karl"},{"full_name":"Messal, Hendrik A.","last_name":"Messal","first_name":"Hendrik A."},{"first_name":"Sharif","last_name":"Iqbal","full_name":"Iqbal, Sharif"},{"first_name":"Dustin J.","last_name":"Flanagan","full_name":"Flanagan, Dustin J."},{"first_name":"David J.","full_name":"Huels, David J.","last_name":"Huels"},{"last_name":"Rojas-Rodríguez","full_name":"Rojas-Rodríguez, Felipe","first_name":"Felipe"},{"first_name":"Miguel","full_name":"Vizoso, Miguel","last_name":"Vizoso"},{"first_name":"Maria","full_name":"Kasper, Maria","last_name":"Kasper"},{"first_name":"Owen J.","full_name":"Sansom, Owen J.","last_name":"Sansom"},{"last_name":"Snippert","full_name":"Snippert, Hugo J.","first_name":"Hugo J."},{"first_name":"Prisca","full_name":"Liberali, Prisca","last_name":"Liberali"},{"last_name":"Simons","full_name":"Simons, Benjamin D.","first_name":"Benjamin D."},{"first_name":"Pekka","last_name":"Katajisto","full_name":"Katajisto, Pekka"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo"},{"full_name":"van Rheenen, Jacco","last_name":"van Rheenen","first_name":"Jacco"}],"article_processing_charge":"No","external_id":{"isi":["000824430000004"],"pmid":["35831497"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"mla":"Azkanaz, Maria, et al. “Retrograde Movements Determine Effective Stem Cell Numbers in the Intestine.” Nature, vol. 607, no. 7919, Springer Nature, 2022, pp. 548–54, doi:10.1038/s41586-022-04962-0.","apa":"Azkanaz, M., Corominas-Murtra, B., Ellenbroek, S. I. J., Bruens, L., Webb, A. T., Laskaris, D., … van Rheenen, J. (2022). Retrograde movements determine effective stem cell numbers in the intestine. Nature. Springer Nature. https://doi.org/10.1038/s41586-022-04962-0","ama":"Azkanaz M, Corominas-Murtra B, Ellenbroek SIJ, et al. Retrograde movements determine effective stem cell numbers in the intestine. Nature. 2022;607(7919):548-554. doi:10.1038/s41586-022-04962-0","ieee":"M. Azkanaz et al., “Retrograde movements determine effective stem cell numbers in the intestine,” Nature, vol. 607, no. 7919. Springer Nature, pp. 548–554, 2022.","short":"M. Azkanaz, B. Corominas-Murtra, S.I.J. Ellenbroek, L. Bruens, A.T. Webb, D. Laskaris, K.C. Oost, S.J.A. Lafirenze, K. Annusver, H.A. Messal, S. Iqbal, D.J. Flanagan, D.J. Huels, F. Rojas-Rodríguez, M. Vizoso, M. Kasper, O.J. Sansom, H.J. Snippert, P. Liberali, B.D. Simons, P. Katajisto, E.B. Hannezo, J. van Rheenen, Nature 607 (2022) 548–554.","chicago":"Azkanaz, Maria, Bernat Corominas-Murtra, Saskia I. J. Ellenbroek, Lotte Bruens, Anna T. Webb, Dimitrios Laskaris, Koen C. Oost, et al. “Retrograde Movements Determine Effective Stem Cell Numbers in the Intestine.” Nature. Springer Nature, 2022. https://doi.org/10.1038/s41586-022-04962-0.","ista":"Azkanaz M, Corominas-Murtra B, Ellenbroek SIJ, Bruens L, Webb AT, Laskaris D, Oost KC, Lafirenze SJA, Annusver K, Messal HA, Iqbal S, Flanagan DJ, Huels DJ, Rojas-Rodríguez F, Vizoso M, Kasper M, Sansom OJ, Snippert HJ, Liberali P, Simons BD, Katajisto P, Hannezo EB, van Rheenen J. 2022. Retrograde movements determine effective stem cell numbers in the intestine. Nature. 607(7919), 548–554."},"month":"07","intvolume":" 607","scopus_import":"1","main_file_link":[{"url":"https://helda.helsinki.fi/items/94433455-4854-45c0-9de8-7326caea8780","open_access":"1"}],"oa_version":"Submitted Version","pmid":1,"abstract":[{"lang":"eng","text":"The morphology and functionality of the epithelial lining differ along the intestinal tract, but tissue renewal at all sites is driven by stem cells at the base of crypts1,2,3. Whether stem cell numbers and behaviour vary at different sites is unknown. Here we show using intravital microscopy that, despite similarities in the number and distribution of proliferative cells with an Lgr5 signature in mice, small intestinal crypts contain twice as many effective stem cells as large intestinal crypts. We find that, although passively displaced by a conveyor-belt-like upward movement, small intestinal cells positioned away from the crypt base can function as long-term effective stem cells owing to Wnt-dependent retrograde cellular movement. By contrast, the near absence of retrograde movement in the large intestine restricts cell repositioning, leading to a reduction in effective stem cell number. Moreover, after suppression of the retrograde movement in the small intestine, the number of effective stem cells is reduced, and the rate of monoclonal conversion of crypts is accelerated. Together, these results show that the number of effective stem cells is determined by active retrograde movement, revealing a new channel of stem cell regulation that can be experimentally and pharmacologically manipulated."}],"issue":"7919","volume":607,"related_material":{"link":[{"relation":"software","url":"https://github.com/JaccovanRheenenLab/Retrograde_movement_Azkanaz_Nature_2022"}]},"ec_funded":1,"language":[{"iso":"eng"}],"publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"publication_status":"published","status":"public","keyword":["Multidisciplinary"],"article_type":"original","type":"journal_article","_id":"12274","department":[{"_id":"EdHa"}],"date_updated":"2023-10-03T11:16:30Z"},{"status":"public","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"type":"research_data_reference","_id":"13068","title":"Spatiotemporal dynamics of self-organized branching in pancreas-derived organoids","department":[{"_id":"EdHa"}],"article_processing_charge":"No","author":[{"full_name":"Randriamanantsoa, Samuel","last_name":"Randriamanantsoa","first_name":"Samuel"},{"first_name":"Aristeidis","full_name":"Papargyriou, Aristeidis","last_name":"Papargyriou"},{"last_name":"Maurer","full_name":"Maurer, Carlo","first_name":"Carlo"},{"full_name":"Peschke, Katja","last_name":"Peschke","first_name":"Katja"},{"first_name":"Maximilian","last_name":"Schuster","full_name":"Schuster, Maximilian"},{"full_name":"Zecchin, Giulia","last_name":"Zecchin","first_name":"Giulia"},{"full_name":"Steiger, Katja","last_name":"Steiger","first_name":"Katja"},{"first_name":"Rupert","full_name":"Öllinger, Rupert","last_name":"Öllinger"},{"last_name":"Saur","full_name":"Saur, Dieter","first_name":"Dieter"},{"first_name":"Christina","last_name":"Scheel","full_name":"Scheel, Christina"},{"last_name":"Rad","full_name":"Rad, Roland","first_name":"Roland"},{"last_name":"Hannezo","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"},{"first_name":"Maximilian","last_name":"Reichert","full_name":"Reichert, Maximilian"},{"first_name":"Andreas R.","last_name":"Bausch","full_name":"Bausch, Andreas R."}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","ddc":["570"],"date_updated":"2023-08-04T09:25:23Z","citation":{"ama":"Randriamanantsoa S, Papargyriou A, Maurer C, et al. Spatiotemporal dynamics of self-organized branching in pancreas-derived organoids. 2021. doi:10.5281/ZENODO.5148117","apa":"Randriamanantsoa, S., Papargyriou, A., Maurer, C., Peschke, K., Schuster, M., Zecchin, G., … Bausch, A. R. (2021). Spatiotemporal dynamics of self-organized branching in pancreas-derived organoids. Zenodo. https://doi.org/10.5281/ZENODO.5148117","ieee":"S. Randriamanantsoa et al., “Spatiotemporal dynamics of self-organized branching in pancreas-derived organoids.” Zenodo, 2021.","short":"S. Randriamanantsoa, A. Papargyriou, C. Maurer, K. Peschke, M. Schuster, G. Zecchin, K. Steiger, R. Öllinger, D. Saur, C. Scheel, R. Rad, E.B. Hannezo, M. Reichert, A.R. Bausch, (2021).","mla":"Randriamanantsoa, Samuel, et al. Spatiotemporal Dynamics of Self-Organized Branching in Pancreas-Derived Organoids. Zenodo, 2021, doi:10.5281/ZENODO.5148117.","ista":"Randriamanantsoa S, Papargyriou A, Maurer C, Peschke K, Schuster M, Zecchin G, Steiger K, Öllinger R, Saur D, Scheel C, Rad R, Hannezo EB, Reichert M, Bausch AR. 2021. Spatiotemporal dynamics of self-organized branching in pancreas-derived organoids, Zenodo, 10.5281/ZENODO.5148117.","chicago":"Randriamanantsoa, Samuel, Aristeidis Papargyriou, Carlo Maurer, Katja Peschke, Maximilian Schuster, Giulia Zecchin, Katja Steiger, et al. “Spatiotemporal Dynamics of Self-Organized Branching in Pancreas-Derived Organoids.” Zenodo, 2021. https://doi.org/10.5281/ZENODO.5148117."},"month":"07","oa":1,"main_file_link":[{"url":"https://doi.org/10.5281/zenodo.6577226","open_access":"1"}],"publisher":"Zenodo","oa_version":"Published Version","abstract":[{"lang":"eng","text":"Source data and source code for the graphs in \"Spatiotemporal dynamics of self-organized branching pancreatic cancer-derived organoids\"."}],"date_created":"2023-05-23T16:39:24Z","date_published":"2021-07-30T00:00:00Z","related_material":{"record":[{"id":"12217","status":"public","relation":"used_in_publication"}]},"doi":"10.5281/ZENODO.5148117","day":"30","year":"2021"},{"project":[{"call_identifier":"FWF","_id":"268294B6-B435-11E9-9278-68D0E5697425","name":"Active mechano-chemical description of the cell cytoskeleton","grant_number":"P31639"},{"name":"Design Principles of Branching Morphogenesis","grant_number":"851288","_id":"05943252-7A3F-11EA-A408-12923DDC885E","call_identifier":"H2020"},{"name":"International IST Doctoral Program","grant_number":"665385","_id":"2564DBCA-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"}],"citation":{"mla":"Boocock, Daniel R., et al. “Theory of Mechanochemical Patterning and Optimal Migration in Cell Monolayers.” Nature Physics, vol. 17, Springer Nature, 2021, pp. 267–74, doi:10.1038/s41567-020-01037-7.","short":"D.R. Boocock, N. Hino, N. Ruzickova, T. Hirashima, E.B. Hannezo, Nature Physics 17 (2021) 267–274.","ieee":"D. R. Boocock, N. Hino, N. Ruzickova, T. Hirashima, and E. B. Hannezo, “Theory of mechanochemical patterning and optimal migration in cell monolayers,” Nature Physics, vol. 17. Springer Nature, pp. 267–274, 2021.","apa":"Boocock, D. R., Hino, N., Ruzickova, N., Hirashima, T., & Hannezo, E. B. (2021). Theory of mechanochemical patterning and optimal migration in cell monolayers. Nature Physics. Springer Nature. https://doi.org/10.1038/s41567-020-01037-7","ama":"Boocock DR, Hino N, Ruzickova N, Hirashima T, Hannezo EB. Theory of mechanochemical patterning and optimal migration in cell monolayers. Nature Physics. 2021;17:267-274. doi:10.1038/s41567-020-01037-7","chicago":"Boocock, Daniel R, Naoya Hino, Natalia Ruzickova, Tsuyoshi Hirashima, and Edouard B Hannezo. “Theory of Mechanochemical Patterning and Optimal Migration in Cell Monolayers.” Nature Physics. Springer Nature, 2021. https://doi.org/10.1038/s41567-020-01037-7.","ista":"Boocock DR, Hino N, Ruzickova N, Hirashima T, Hannezo EB. 2021. Theory of mechanochemical patterning and optimal migration in cell monolayers. Nature Physics. 17, 267–274."},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","author":[{"last_name":"Boocock","orcid":"0000-0002-1585-2631","full_name":"Boocock, Daniel R","first_name":"Daniel R","id":"453AF628-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Hino","full_name":"Hino, Naoya","first_name":"Naoya"},{"first_name":"Natalia","id":"D2761128-D73D-11E9-A1BF-BA0DE6697425","last_name":"Ruzickova","full_name":"Ruzickova, Natalia"},{"full_name":"Hirashima, Tsuyoshi","last_name":"Hirashima","first_name":"Tsuyoshi"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561"}],"external_id":{"isi":["000573519500002"]},"article_processing_charge":"No","title":"Theory of mechanochemical patterning and optimal migration in cell monolayers","acknowledgement":"We would like to thank G. Tkacik and all of the members of the Hannezo and Hirashima groups for useful discussions, X. Trepat for help on traction force microscopy and M. Matsuda for use of the lab facility. E.H. acknowledges grants from the Austrian Science Fund (FWF) (P 31639) and the European Research Council (851288). T.H. acknowledges a grant from JST, PRESTO (JPMJPR1949). This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 665385 (to D.B.), from JSPS KAKENHI grant no. 17J02107 (to N.H.) and from the SPIRITS 2018 of Kyoto University (to E.H. and T.H.).","quality_controlled":"1","publisher":"Springer Nature","oa":1,"isi":1,"year":"2021","day":"01","publication":"Nature Physics","page":"267-274","doi":"10.1038/s41567-020-01037-7","date_published":"2021-02-01T00:00:00Z","date_created":"2020-10-04T22:01:37Z","_id":"8602","article_type":"original","type":"journal_article","status":"public","date_updated":"2023-08-04T11:02:41Z","department":[{"_id":"EdHa"}],"abstract":[{"lang":"eng","text":"Collective cell migration offers a rich field of study for non-equilibrium physics and cellular biology, revealing phenomena such as glassy dynamics, pattern formation and active turbulence. However, how mechanical and chemical signalling are integrated at the cellular level to give rise to such collective behaviours remains unclear. We address this by focusing on the highly conserved phenomenon of spatiotemporal waves of density and extracellular signal-regulated kinase (ERK) activation, which appear both in vitro and in vivo during collective cell migration and wound healing. First, we propose a biophysical theory, backed by mechanical and optogenetic perturbation experiments, showing that patterns can be quantitatively explained by a mechanochemical coupling between active cellular tensions and the mechanosensitive ERK pathway. Next, we demonstrate how this biophysical mechanism can robustly induce long-ranged order and migration in a desired orientation, and we determine the theoretically optimal wavelength and period for inducing maximal migration towards free edges, which fits well with experimentally observed dynamics. We thereby provide a bridge between the biophysical origin of spatiotemporal instabilities and the design principles of robust and efficient long-ranged migration."}],"oa_version":"Preprint","scopus_import":"1","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2020.05.15.096479"}],"month":"02","intvolume":" 17","publication_identifier":{"issn":["17452473"],"eissn":["17452481"]},"publication_status":"published","language":[{"iso":"eng"}],"volume":17,"related_material":{"record":[{"relation":"dissertation_contains","id":"12964","status":"public"}],"link":[{"description":"News on IST Homepage","url":"https://ist.ac.at/en/news/wound-healing-waves/","relation":"press_release"}]},"ec_funded":1},{"status":"public","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_type":"original","type":"journal_article","_id":"9244","file_date_updated":"2021-03-22T08:50:33Z","department":[{"_id":"EdHa"}],"ddc":["570"],"date_updated":"2023-08-07T14:12:54Z","intvolume":" 10","month":"02","scopus_import":"1","oa_version":"Published Version","pmid":1,"abstract":[{"text":"Organ function depends on tissues adopting the correct architecture. However, insights into organ architecture are currently hampered by an absence of standardized quantitative 3D analysis. We aimed to develop a robust technology to visualize, digitalize, and segment the architecture of two tubular systems in 3D: double resin casting micro computed tomography (DUCT). As proof of principle, we applied DUCT to a mouse model for Alagille syndrome (Jag1Ndr/Ndr mice), characterized by intrahepatic bile duct paucity, that can spontaneously generate a biliary system in adulthood. DUCT identified increased central biliary branching and peripheral bile duct tortuosity as two compensatory processes occurring in distinct regions of Jag1Ndr/Ndr liver, leading to full reconstitution of wild-type biliary volume and phenotypic recovery. DUCT is thus a powerful new technology for 3D analysis, which can reveal novel phenotypes and provide a standardized method of defining liver architecture in mouse models.","lang":"eng"}],"ec_funded":1,"volume":10,"language":[{"iso":"eng"}],"file":[{"file_size":9259690,"date_updated":"2021-03-22T08:50:33Z","creator":"dernst","file_name":"2021_eLife_Hankeova.pdf","date_created":"2021-03-22T08:50:33Z","content_type":"application/pdf","relation":"main_file","access_level":"open_access","success":1,"checksum":"20ccf4dfe46c48cf986794c8bf4fd1cb","file_id":"9271"}],"publication_status":"published","publication_identifier":{"eissn":["2050084X"]},"project":[{"_id":"05943252-7A3F-11EA-A408-12923DDC885E","call_identifier":"H2020","grant_number":"851288","name":"Design Principles of Branching Morphogenesis"}],"article_number":"e60916","title":"DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for alagille syndrome","article_processing_charge":"No","external_id":{"pmid":["33635272"],"isi":["000625357100001"]},"author":[{"full_name":"Hankeova, Simona","last_name":"Hankeova","first_name":"Simona"},{"last_name":"Salplachta","full_name":"Salplachta, Jakub","first_name":"Jakub"},{"first_name":"Tomas","last_name":"Zikmund","full_name":"Zikmund, Tomas"},{"first_name":"Michaela","full_name":"Kavkova, Michaela","last_name":"Kavkova"},{"last_name":"Van Hul","full_name":"Van Hul, Noémi","first_name":"Noémi"},{"full_name":"Brinek, Adam","last_name":"Brinek","first_name":"Adam"},{"first_name":"Veronika","full_name":"Smekalova, Veronika","last_name":"Smekalova"},{"last_name":"Laznovsky","full_name":"Laznovsky, Jakub","first_name":"Jakub"},{"last_name":"Dawit","full_name":"Dawit, Feven","first_name":"Feven"},{"full_name":"Jaros, Josef","last_name":"Jaros","first_name":"Josef"},{"full_name":"Bryja, Vítězslav","last_name":"Bryja","first_name":"Vítězslav"},{"first_name":"Urban","full_name":"Lendahl, Urban","last_name":"Lendahl"},{"first_name":"Ewa","last_name":"Ellis","full_name":"Ellis, Ewa"},{"first_name":"Antal","full_name":"Nemeth, Antal","last_name":"Nemeth"},{"full_name":"Fischler, Björn","last_name":"Fischler","first_name":"Björn"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","last_name":"Hannezo","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B"},{"last_name":"Kaiser","full_name":"Kaiser, Jozef","first_name":"Jozef"},{"last_name":"Andersson","full_name":"Andersson, Emma Rachel","first_name":"Emma Rachel"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"mla":"Hankeova, Simona, et al. “DUCT Reveals Architectural Mechanisms Contributing to Bile Duct Recovery in a Mouse Model for Alagille Syndrome.” ELife, vol. 10, e60916, eLife Sciences Publications, 2021, doi:10.7554/eLife.60916.","ama":"Hankeova S, Salplachta J, Zikmund T, et al. DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for alagille syndrome. eLife. 2021;10. doi:10.7554/eLife.60916","apa":"Hankeova, S., Salplachta, J., Zikmund, T., Kavkova, M., Van Hul, N., Brinek, A., … Andersson, E. R. (2021). DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for alagille syndrome. ELife. eLife Sciences Publications. https://doi.org/10.7554/eLife.60916","ieee":"S. Hankeova et al., “DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for alagille syndrome,” eLife, vol. 10. eLife Sciences Publications, 2021.","short":"S. Hankeova, J. Salplachta, T. Zikmund, M. Kavkova, N. Van Hul, A. Brinek, V. Smekalova, J. Laznovsky, F. Dawit, J. Jaros, V. Bryja, U. Lendahl, E. Ellis, A. Nemeth, B. Fischler, E.B. Hannezo, J. Kaiser, E.R. Andersson, ELife 10 (2021).","chicago":"Hankeova, Simona, Jakub Salplachta, Tomas Zikmund, Michaela Kavkova, Noémi Van Hul, Adam Brinek, Veronika Smekalova, et al. “DUCT Reveals Architectural Mechanisms Contributing to Bile Duct Recovery in a Mouse Model for Alagille Syndrome.” ELife. eLife Sciences Publications, 2021. https://doi.org/10.7554/eLife.60916.","ista":"Hankeova S, Salplachta J, Zikmund T, Kavkova M, Van Hul N, Brinek A, Smekalova V, Laznovsky J, Dawit F, Jaros J, Bryja V, Lendahl U, Ellis E, Nemeth A, Fischler B, Hannezo EB, Kaiser J, Andersson ER. 2021. DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for alagille syndrome. eLife. 10, e60916."},"oa":1,"quality_controlled":"1","publisher":"eLife Sciences Publications","acknowledgement":"Work in ERA lab is supported by the Swedish Research Council, the Center of Innovative Medicine (CIMED) Grant, Karolinska Institutet, and the Heart and Lung Foundation, and\r\nthe Daniel Alagille Award from the European Association for the Study of the Liver. One project in ERA lab is funded by ModeRNA, unrelated to this project. The funders have no role in the design or interpretation of the work. SH has been supported by a KI-MU PhD student program, and by a Wera Ekstro¨m Foundation Scholarship. We are grateful for support from Tornspiran foundation to NVH. JK: This research was carried out under the project CEITEC 2020 (LQ1601) with financial support from the Ministry of Education, Youth and Sports of the Czech Republic under the National Sustainability Programme II and CzechNanoLab Research Infrastructure supported by MEYS CR (LM2018110) . UL: The financial support from the Swedish Research Council and ICMC (Integrated CardioMetabolic Center) is acknowledged. JJ: The work was supported by the Grant Agency of Masaryk University (project no. MUNI/A/1565/2018). We thank Kari Huppert and Stacey Huppert for their expertise and help regarding bile duct cannulation and their laboratory hospitality. We also thank Nadja Schultz and Charlotte L Mattsson for their help with common bile duct cannulation. We thank Daniel Holl for his help with trachea cannulation. We thank Nikos Papadogiannakis for his assistance with mild Alagille biopsy samples and discussion. We thank Karolinska Biomedicum Imaging Core, especially Shigeaki Kanatani for his help with image analysis. We thank Jan Masek and Carolina Gutierrez for their scientific input in manuscript writing. We thank Peter Ranefall and the BioImage Informatics (SciLife national facility) for their help writing parts of the MATLAB pipeline.\r\nThe TROMA-III antibody developed by Rolf Kemler was obtained from the Developmental Studies Hybridoma (DSHB) Bank developed under the auspices of NICHD and maintained by The University of Iowa, Department of Biological Sciences, Iowa City, IA52242. We thank Goncalo M Brito for all illustrations. This work was supported by the European Union (European Research Council Starting grant 851288 to E.H.).","date_created":"2021-03-14T23:01:34Z","doi":"10.7554/eLife.60916","date_published":"2021-02-26T00:00:00Z","publication":"eLife","day":"26","year":"2021","has_accepted_license":"1","isi":1},{"_id":"9306","type":"journal_article","article_type":"original","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"status":"public","date_updated":"2023-08-07T14:32:28Z","ddc":["576"],"department":[{"_id":"EdHa"}],"file_date_updated":"2021-04-06T10:39:08Z","abstract":[{"lang":"eng","text":"Assemblies of actin and its regulators underlie the dynamic morphology of all eukaryotic cells. To understand how actin regulatory proteins work together to generate actin-rich structures such as filopodia, we analyzed the localization of diverse actin regulators within filopodia in Drosophila embryos and in a complementary in vitro system of filopodia-like structures (FLSs). We found that the composition of the regulatory protein complex where actin is incorporated (the filopodial tip complex) is remarkably heterogeneous both in vivo and in vitro. Our data reveal that different pairs of proteins correlate with each other and with actin bundle length, suggesting the presence of functional subcomplexes. This is consistent with a theoretical framework where three or more redundant subcomplexes join the tip complex stochastically, with any two being sufficient to drive filopodia formation. We provide an explanation for the observed heterogeneity and suggest that a mechanism based on multiple components allows stereotypical filopodial dynamics to arise from diverse upstream signaling pathways."}],"pmid":1,"oa_version":"Published Version","scopus_import":"1","month":"03","intvolume":" 220","publication_identifier":{"eissn":["15408140"]},"publication_status":"published","file":[{"file_name":"2021_JCB_Dobramysl.pdf","date_created":"2021-04-06T10:39:08Z","file_size":9019720,"date_updated":"2021-04-06T10:39:08Z","creator":"dernst","success":1,"checksum":"4739ffd90f2c7e05ac5b00f057c58aa2","file_id":"9310","content_type":"application/pdf","relation":"main_file","access_level":"open_access"}],"language":[{"iso":"eng"}],"issue":"4","volume":220,"article_number":"e202003052","project":[{"grant_number":"P31639","name":"Active mechano-chemical description of the cell cytoskeleton","_id":"268294B6-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"}],"citation":{"mla":"Dobramysl, Ulrich, et al. “Stochastic Combinations of Actin Regulatory Proteins Are Sufficient to Drive Filopodia Formation.” Journal of Cell Biology, vol. 220, no. 4, e202003052, Rockefeller University Press, 2021, doi:10.1083/jcb.202003052.","ieee":"U. Dobramysl et al., “Stochastic combinations of actin regulatory proteins are sufficient to drive filopodia formation,” Journal of Cell Biology, vol. 220, no. 4. Rockefeller University Press, 2021.","short":"U. Dobramysl, I.K. Jarsch, Y. Inoue, H. Shimo, B. Richier, J.R. Gadsby, J. Mason, A. Szałapak, P.S. Ioannou, G.P. Correia, A. Walrant, R. Butler, E.B. Hannezo, B.D. Simons, J.L. Gallop, Journal of Cell Biology 220 (2021).","ama":"Dobramysl U, Jarsch IK, Inoue Y, et al. Stochastic combinations of actin regulatory proteins are sufficient to drive filopodia formation. Journal of Cell Biology. 2021;220(4). doi:10.1083/jcb.202003052","apa":"Dobramysl, U., Jarsch, I. K., Inoue, Y., Shimo, H., Richier, B., Gadsby, J. R., … Gallop, J. L. (2021). Stochastic combinations of actin regulatory proteins are sufficient to drive filopodia formation. Journal of Cell Biology. Rockefeller University Press. https://doi.org/10.1083/jcb.202003052","chicago":"Dobramysl, Ulrich, Iris Katharina Jarsch, Yoshiko Inoue, Hanae Shimo, Benjamin Richier, Jonathan R. Gadsby, Julia Mason, et al. “Stochastic Combinations of Actin Regulatory Proteins Are Sufficient to Drive Filopodia Formation.” Journal of Cell Biology. Rockefeller University Press, 2021. https://doi.org/10.1083/jcb.202003052.","ista":"Dobramysl U, Jarsch IK, Inoue Y, Shimo H, Richier B, Gadsby JR, Mason J, Szałapak A, Ioannou PS, Correia GP, Walrant A, Butler R, Hannezo EB, Simons BD, Gallop JL. 2021. Stochastic combinations of actin regulatory proteins are sufficient to drive filopodia formation. Journal of Cell Biology. 220(4), e202003052."},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","author":[{"full_name":"Dobramysl, Ulrich","last_name":"Dobramysl","first_name":"Ulrich"},{"first_name":"Iris Katharina","full_name":"Jarsch, Iris Katharina","last_name":"Jarsch"},{"first_name":"Yoshiko","last_name":"Inoue","full_name":"Inoue, Yoshiko"},{"first_name":"Hanae","full_name":"Shimo, Hanae","last_name":"Shimo"},{"first_name":"Benjamin","last_name":"Richier","full_name":"Richier, Benjamin"},{"first_name":"Jonathan R.","full_name":"Gadsby, Jonathan R.","last_name":"Gadsby"},{"full_name":"Mason, Julia","last_name":"Mason","first_name":"Julia"},{"first_name":"Alicja","full_name":"Szałapak, Alicja","last_name":"Szałapak"},{"first_name":"Pantelis Savvas","last_name":"Ioannou","full_name":"Ioannou, Pantelis Savvas"},{"last_name":"Correia","full_name":"Correia, Guilherme Pereira","first_name":"Guilherme Pereira"},{"full_name":"Walrant, Astrid","last_name":"Walrant","first_name":"Astrid"},{"first_name":"Richard","last_name":"Butler","full_name":"Butler, Richard"},{"first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561"},{"first_name":"Benjamin D.","full_name":"Simons, Benjamin D.","last_name":"Simons"},{"first_name":"Jennifer L.","last_name":"Gallop","full_name":"Gallop, Jennifer L."}],"article_processing_charge":"No","external_id":{"pmid":["33740033"],"isi":["000663160600002"]},"title":"Stochastic combinations of actin regulatory proteins are sufficient to drive filopodia formation","acknowledgement":"This work was supported by European Research Council grant 281971, Wellcome Trust Research Career Development Fellowship WT095829AIA and Wellcome Trust Senior Research\r\nFellowship 219482/Z/19/Z to J.L. Gallop, a Wellcome Trust Senior Investigator Award 098357 to B.D. Simons, and an Austrian Science Fund grant (P31639) to E. Hannezo. We acknowledge\r\ncore funding by the Wellcome Trust (092096) and Cancer Research UK (C6946/A14492). U. Dobramysl was supported by a Wellcome Trust Junior Interdisciplinary Fellowship grant\r\n(105602/Z/14/Z) and a Herchel Smith Postdoctoral Fellowship. H. Shimo was supported by a Funai Foundation Overseas scholarship.","publisher":"Rockefeller University Press","quality_controlled":"1","oa":1,"isi":1,"has_accepted_license":"1","year":"2021","day":"19","publication":"Journal of Cell Biology","date_published":"2021-03-19T00:00:00Z","doi":"10.1083/jcb.202003052","date_created":"2021-04-04T22:01:21Z"},{"project":[{"_id":"260F1432-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","grant_number":"742573"},{"_id":"05943252-7A3F-11EA-A408-12923DDC885E","call_identifier":"H2020","name":"Design Principles of Branching Morphogenesis","grant_number":"851288"},{"name":"Tissue material properties in embryonic development","grant_number":"V00736","_id":"2693FD8C-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"}],"citation":{"mla":"Petridou, Nicoletta, et al. “Rigidity Percolation Uncovers a Structural Basis for Embryonic Tissue Phase Transitions.” Cell, vol. 184, no. 7, Elsevier, 2021, p. 1914–1928.e19, doi:10.1016/j.cell.2021.02.017.","ama":"Petridou N, Corominas-Murtra B, Heisenberg C-PJ, Hannezo EB. Rigidity percolation uncovers a structural basis for embryonic tissue phase transitions. Cell. 2021;184(7):1914-1928.e19. doi:10.1016/j.cell.2021.02.017","apa":"Petridou, N., Corominas-Murtra, B., Heisenberg, C.-P. J., & Hannezo, E. B. (2021). Rigidity percolation uncovers a structural basis for embryonic tissue phase transitions. Cell. Elsevier. https://doi.org/10.1016/j.cell.2021.02.017","ieee":"N. Petridou, B. Corominas-Murtra, C.-P. J. Heisenberg, and E. B. Hannezo, “Rigidity percolation uncovers a structural basis for embryonic tissue phase transitions,” Cell, vol. 184, no. 7. Elsevier, p. 1914–1928.e19, 2021.","short":"N. Petridou, B. Corominas-Murtra, C.-P.J. Heisenberg, E.B. Hannezo, Cell 184 (2021) 1914–1928.e19.","chicago":"Petridou, Nicoletta, Bernat Corominas-Murtra, Carl-Philipp J Heisenberg, and Edouard B Hannezo. “Rigidity Percolation Uncovers a Structural Basis for Embryonic Tissue Phase Transitions.” Cell. Elsevier, 2021. https://doi.org/10.1016/j.cell.2021.02.017.","ista":"Petridou N, Corominas-Murtra B, Heisenberg C-PJ, Hannezo EB. 2021. Rigidity percolation uncovers a structural basis for embryonic tissue phase transitions. Cell. 184(7), 1914–1928.e19."},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","article_processing_charge":"No","external_id":{"pmid":["33730596"],"isi":["000636734000022"]},"author":[{"last_name":"Petridou","full_name":"Petridou, Nicoletta","orcid":"0000-0002-8451-1195","first_name":"Nicoletta","id":"2A003F6C-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Corominas-Murtra","full_name":"Corominas-Murtra, Bernat","orcid":"0000-0001-9806-5643","id":"43BE2298-F248-11E8-B48F-1D18A9856A87","first_name":"Bernat"},{"last_name":"Heisenberg","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561"}],"title":"Rigidity percolation uncovers a structural basis for embryonic tissue phase transitions","acknowledgement":"We thank Carl Goodrich and the members of the Heisenberg and Hannezo groups, in particular Reka Korei, for help, technical advice, and discussions; and the Bioimaging and zebrafish facilities of the IST Austria for continuous support. This work was supported by the Elise Richter Program of Austrian Science Fund (FWF) to N.I.P. ( V 736-B26 ) and the European Union (European Research Council Advanced Grant 742573 to C.-P.H. and European Research Council Starting Grant 851288 to E.H.).","oa":1,"publisher":"Elsevier","quality_controlled":"1","year":"2021","has_accepted_license":"1","isi":1,"publication":"Cell","day":"01","page":"1914-1928.e19","date_created":"2021-04-11T22:01:14Z","doi":"10.1016/j.cell.2021.02.017","date_published":"2021-04-01T00:00:00Z","_id":"9316","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_type":"original","type":"journal_article","status":"public","date_updated":"2023-08-07T14:33:59Z","ddc":["570"],"department":[{"_id":"CaHe"},{"_id":"EdHa"}],"file_date_updated":"2021-06-08T10:04:10Z","abstract":[{"text":"Embryo morphogenesis is impacted by dynamic changes in tissue material properties, which have been proposed to occur via processes akin to phase transitions (PTs). Here, we show that rigidity percolation provides a simple and robust theoretical framework to predict material/structural PTs of embryonic tissues from local cell connectivity. By using percolation theory, combined with directly monitoring dynamic changes in tissue rheology and cell contact mechanics, we demonstrate that the zebrafish blastoderm undergoes a genuine rigidity PT, brought about by a small reduction in adhesion-dependent cell connectivity below a critical value. We quantitatively predict and experimentally verify hallmarks of PTs, including power-law exponents and associated discontinuities of macroscopic observables. Finally, we show that this uniform PT depends on blastoderm cells undergoing meta-synchronous divisions causing random and, consequently, uniform changes in cell connectivity. Collectively, our theoretical and experimental findings reveal the structural basis of material PTs in an organismal context.","lang":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"}],"pmid":1,"oa_version":"Published Version","scopus_import":"1","intvolume":" 184","month":"04","publication_status":"published","publication_identifier":{"issn":["00928674"],"eissn":["10974172"]},"language":[{"iso":"eng"}],"file":[{"file_size":11405875,"date_updated":"2021-06-08T10:04:10Z","creator":"cziletti","file_name":"2021_Cell_Petridou.pdf","date_created":"2021-06-08T10:04:10Z","content_type":"application/pdf","relation":"main_file","access_level":"open_access","success":1,"checksum":"1e5295fbd9c2a459173ec45a0e8a7c2e","file_id":"9534"}],"ec_funded":1,"related_material":{"link":[{"description":"News on IST Homepage","relation":"press_release","url":"https://ist.ac.at/en/news/embryonic-tissue-undergoes-phase-transition/"}]},"volume":184,"issue":"7"},{"article_number":"041501","project":[{"call_identifier":"H2020","_id":"B6FC0238-B512-11E9-945C-1524E6697425","name":"Coordination of Patterning And Growth In the Spinal Cord","grant_number":"680037"},{"_id":"268294B6-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Active mechano-chemical description of the cell cytoskeleton","grant_number":"P31639"},{"_id":"05943252-7A3F-11EA-A408-12923DDC885E","call_identifier":"H2020","name":"Design Principles of Branching Morphogenesis","grant_number":"851288"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"ista":"Lenne PF, Munro E, Heemskerk I, Warmflash A, Bocanegra L, Kishi K, Kicheva A, Long Y, Fruleux A, Boudaoud A, Saunders TE, Caldarelli P, Michaut A, Gros J, Maroudas-Sacks Y, Keren K, Hannezo EB, Gartner ZJ, Stormo B, Gladfelter A, Rodrigues A, Shyer A, Minc N, Maître JL, Di Talia S, Khamaisi B, Sprinzak D, Tlili S. 2021. Roadmap for the multiscale coupling of biochemical and mechanical signals during development. Physical biology. 18(4), 041501.","chicago":"Lenne, Pierre François, Edwin Munro, Idse Heemskerk, Aryeh Warmflash, Laura Bocanegra, Kasumi Kishi, Anna Kicheva, et al. “Roadmap for the Multiscale Coupling of Biochemical and Mechanical Signals during Development.” Physical Biology. IOP Publishing, 2021. https://doi.org/10.1088/1478-3975/abd0db.","short":"P.F. Lenne, E. Munro, I. Heemskerk, A. Warmflash, L. Bocanegra, K. Kishi, A. Kicheva, Y. Long, A. Fruleux, A. Boudaoud, T.E. Saunders, P. Caldarelli, A. Michaut, J. Gros, Y. Maroudas-Sacks, K. Keren, E.B. Hannezo, Z.J. Gartner, B. Stormo, A. Gladfelter, A. Rodrigues, A. Shyer, N. Minc, J.L. Maître, S. Di Talia, B. Khamaisi, D. Sprinzak, S. Tlili, Physical Biology 18 (2021).","ieee":"P. F. Lenne et al., “Roadmap for the multiscale coupling of biochemical and mechanical signals during development,” Physical biology, vol. 18, no. 4. IOP Publishing, 2021.","apa":"Lenne, P. F., Munro, E., Heemskerk, I., Warmflash, A., Bocanegra, L., Kishi, K., … Tlili, S. (2021). Roadmap for the multiscale coupling of biochemical and mechanical signals during development. Physical Biology. IOP Publishing. https://doi.org/10.1088/1478-3975/abd0db","ama":"Lenne PF, Munro E, Heemskerk I, et al. Roadmap for the multiscale coupling of biochemical and mechanical signals during development. Physical biology. 2021;18(4). doi:10.1088/1478-3975/abd0db","mla":"Lenne, Pierre François, et al. “Roadmap for the Multiscale Coupling of Biochemical and Mechanical Signals during Development.” Physical Biology, vol. 18, no. 4, 041501, IOP Publishing, 2021, doi:10.1088/1478-3975/abd0db."},"title":"Roadmap for the multiscale coupling of biochemical and mechanical signals during development","author":[{"first_name":"Pierre François","last_name":"Lenne","full_name":"Lenne, Pierre François"},{"last_name":"Munro","full_name":"Munro, Edwin","first_name":"Edwin"},{"first_name":"Idse","full_name":"Heemskerk, Idse","last_name":"Heemskerk"},{"last_name":"Warmflash","full_name":"Warmflash, Aryeh","first_name":"Aryeh"},{"id":"4896F754-F248-11E8-B48F-1D18A9856A87","first_name":"Laura","last_name":"Bocanegra","full_name":"Bocanegra, Laura"},{"full_name":"Kishi, Kasumi","last_name":"Kishi","first_name":"Kasumi","id":"3065DFC4-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Kicheva","orcid":"0000-0003-4509-4998","full_name":"Kicheva, Anna","id":"3959A2A0-F248-11E8-B48F-1D18A9856A87","first_name":"Anna"},{"first_name":"Yuchen","last_name":"Long","full_name":"Long, Yuchen"},{"last_name":"Fruleux","full_name":"Fruleux, Antoine","first_name":"Antoine"},{"full_name":"Boudaoud, Arezki","last_name":"Boudaoud","first_name":"Arezki"},{"full_name":"Saunders, Timothy E.","last_name":"Saunders","first_name":"Timothy E."},{"full_name":"Caldarelli, Paolo","last_name":"Caldarelli","first_name":"Paolo"},{"first_name":"Arthur","full_name":"Michaut, Arthur","last_name":"Michaut"},{"last_name":"Gros","full_name":"Gros, Jerome","first_name":"Jerome"},{"first_name":"Yonit","last_name":"Maroudas-Sacks","full_name":"Maroudas-Sacks, Yonit"},{"first_name":"Kinneret","full_name":"Keren, Kinneret","last_name":"Keren"},{"first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561"},{"last_name":"Gartner","full_name":"Gartner, Zev J.","first_name":"Zev J."},{"first_name":"Benjamin","last_name":"Stormo","full_name":"Stormo, Benjamin"},{"first_name":"Amy","full_name":"Gladfelter, Amy","last_name":"Gladfelter"},{"last_name":"Rodrigues","full_name":"Rodrigues, Alan","first_name":"Alan"},{"first_name":"Amy","last_name":"Shyer","full_name":"Shyer, Amy"},{"first_name":"Nicolas","full_name":"Minc, Nicolas","last_name":"Minc"},{"full_name":"Maître, Jean Léon","last_name":"Maître","first_name":"Jean Léon"},{"first_name":"Stefano","full_name":"Di Talia, Stefano","last_name":"Di Talia"},{"last_name":"Khamaisi","full_name":"Khamaisi, Bassma","first_name":"Bassma"},{"last_name":"Sprinzak","full_name":"Sprinzak, David","first_name":"David"},{"first_name":"Sham","last_name":"Tlili","full_name":"Tlili, Sham"}],"external_id":{"isi":["000640396400001"],"pmid":["33276350"]},"article_processing_charge":"No","acknowledgement":"The AK group is supported by IST Austria and by the ERC under European Union Horizon 2020 research and innovation programme Grant 680037. Apologies to those whose work could not be mentioned due to limited space. We thank all my lab members, both past and present, for stimulating discussion. This work was funded by a Singapore Ministry of Education Tier 3 Grant, MOE2016-T3-1-005. We thank Francis Corson for continuous discussion and collaboration contributing to these views and for figure 4(A). PC is sponsored by the Institut Pasteur and the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie Grant Agreement No. 665807. Research in JG's laboratory is funded by the European Research Council under the European Union's Seventh Framework Programme (FP7/2007-2013)/ERC Grant Agreement No. 337635, Institut Pasteur, CNRS, Cercle FSER, Fondation pour la Recherche Medicale, the Vallee Foundation and the ANR-19-CE-13-0024 Grant. We thank Erez Braun and Alex Mogilner for comments on the manuscript and Niv Ierushalmi for help with figure 5. This project has received funding from the European Union's Horizon 2020 research and innovation programme under Grant Agreement No. ERC-2018-COG Grant 819174-HydraMechanics awarded to KK. EH thanks all lab members, as well as Pierre Recho, Tsuyoshi Hirashima, Diana Pinheiro and Carl-Philip Heisenberg, for fruitful discussions on these topics—and apologize for not being able to cite many very relevant publications due to the strict 10-reference limit. EH acknowledges the support of Austrian Science Fund (FWF) (P 31639) and the European Research Council under the European Union's Horizon 2020 Research and Innovation Programme Grant Agreements (851288). The authors acknowledge the inspiring scientists whose work could not be cited in this perspective due to space constraints; the members of the Gartner Lab for helpful discussions; the Barbara and Gerson Bakar Foundation, the Chan Zuckerberg Biohub Investigators Programme, the National Institute of Health, and the Centre for Cellular Construction, an NSF Science and Technology Centre. The Minc laboratory is currently funded by the CNRS and the European Research Council (CoG Forcaster No. 647073). Research in the lab of J-LM is supported by the Institut Curie, the Centre National de la Recherche Scientifique (CNRS), the Institut National de la Santé Et de la Recherche Médicale (INSERM), and is funded by grants from the ATIP-Avenir programme, the Fondation Schlumberger pour l'Éducation et la Recherche via the Fondation pour la Recherche Médicale, the European Research Council Starting Grant ERC-2017-StG 757557, the European Molecular Biology Organization Young Investigator programme (EMBO YIP), the INSERM transversal programme Human Development Cell Atlas (HuDeCA), Paris Sciences Lettres (PSL) 'nouvelle équipe' and QLife (17-CONV-0005) grants and Labex DEEP (ANR-11-LABX-0044) which are part of the IDEX PSL (ANR-10-IDEX-0001-02). We acknowledge useful discussions with Massimo Vergassola, Sebastian Streichan and my lab members. Work in my laboratory on Drosophila embryogenesis is partly supported by NIH-R01GM122936. The authors acknowledge the support by a grant from the European Research Council (Grant No. 682161). Lenne group is funded by a grant from the 'Investissements d'Avenir' French Government programme managed by the French National Research Agency (ANR-16-CONV-0001) and by the Excellence Initiative of Aix-Marseille University—A*MIDEX, and ANR projects MechaResp (ANR-17-CE13-0032) and AdGastrulo (ANR-19-CE13-0022).","quality_controlled":"1","publisher":"IOP Publishing","oa":1,"day":"14","publication":"Physical biology","has_accepted_license":"1","isi":1,"year":"2021","doi":"10.1088/1478-3975/abd0db","date_published":"2021-04-14T00:00:00Z","date_created":"2021-04-25T22:01:29Z","_id":"9349","status":"public","type":"journal_article","article_type":"original","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"ddc":["570"],"date_updated":"2023-08-08T13:15:46Z","file_date_updated":"2021-04-27T08:38:35Z","department":[{"_id":"AnKi"},{"_id":"EdHa"}],"oa_version":"Published Version","pmid":1,"abstract":[{"lang":"eng","text":"The way in which interactions between mechanics and biochemistry lead to the emergence of complex cell and tissue organization is an old question that has recently attracted renewed interest from biologists, physicists, mathematicians and computer scientists. Rapid advances in optical physics, microscopy and computational image analysis have greatly enhanced our ability to observe and quantify spatiotemporal patterns of signalling, force generation, deformation, and flow in living cells and tissues. Powerful new tools for genetic, biophysical and optogenetic manipulation are allowing us to perturb the underlying machinery that generates these patterns in increasingly sophisticated ways. Rapid advances in theory and computing have made it possible to construct predictive models that describe how cell and tissue organization and dynamics emerge from the local coupling of biochemistry and mechanics. Together, these advances have opened up a wealth of new opportunities to explore how mechanochemical patterning shapes organismal development. In this roadmap, we present a series of forward-looking case studies on mechanochemical patterning in development, written by scientists working at the interface between the physical and biological sciences, and covering a wide range of spatial and temporal scales, organisms, and modes of development. Together, these contributions highlight the many ways in which the dynamic coupling of mechanics and biochemistry shapes biological dynamics: from mechanoenzymes that sense force to tune their activity and motor output, to collectives of cells in tissues that flow and redistribute biochemical signals during development."}],"month":"04","intvolume":" 18","scopus_import":"1","file":[{"file_name":"2021_PhysBio_Lenne.pdf","date_created":"2021-04-27T08:38:35Z","file_size":6296324,"date_updated":"2021-04-27T08:38:35Z","creator":"cziletti","success":1,"file_id":"9355","checksum":"4f52082549d3561c4c15d4d8d84ca5d8","content_type":"application/pdf","relation":"main_file","access_level":"open_access"}],"language":[{"iso":"eng"}],"publication_identifier":{"eissn":["1478-3975"]},"publication_status":"published","related_material":{"record":[{"id":"13081","status":"public","relation":"dissertation_contains"}]},"volume":18,"issue":"4","ec_funded":1},{"day":"21","publication":"Nature Cell Biology","isi":1,"year":"2021","doi":"10.1038/s41556-021-00700-2","date_published":"2021-06-21T00:00:00Z","date_created":"2021-07-04T22:01:25Z","page":"733–744","acknowledgement":"We acknowledge the members of the Lennon-Duménil laboratory for sharing the mouse line of Myh9-GFP. We are grateful to the members of the Liberali laboratory and the FMI facilities for their support. We thank E. Tagliavini for IT support; L. Gelman for assistance and training; S. Bichet and A. Bogucki for helping with histology of mouse tissues; H. Kohler for fluorescence-activated cell sorting; G. Q. G. de Medeiros for maintenance of light-sheet microscopy; M. G. Stadler for scRNA-seq analysis; G. Gay for discussions on the 3D vertex model; the members of the Liberali laboratory, C. P. Heisenberg and C. Tsiairis for reading and providing feedback on the manuscript. Funding: Q.Y. is supported by a Postdoc fellowship from Peter und Taul Engelhorn Stiftung (PTES). This work received funding from the European Research Council (ERC) under the EU Horizon 2020 research and Innovation Programme Grant Agreement no. 758617 (to P.L.), the Swiss National Foundation (SNF) (POOP3_157531, to P.L.) and from the ERC under the EU Horizon 2020 Research and Innovation Program Grant Agreements 851288 (to E.H.) and the Austrian Science Fund (FWF) (P31639, to E.H.).","publisher":"Springer Nature","quality_controlled":"1","oa":1,"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"mla":"Yang, Qiutan, et al. “Cell Fate Coordinates Mechano-Osmotic Forces in Intestinal Crypt Formation.” Nature Cell Biology, vol. 23, Springer Nature, 2021, pp. 733–744, doi:10.1038/s41556-021-00700-2.","apa":"Yang, Q., Xue, S., Chan, C. J., Rempfler, M., Vischi, D., Maurer-Gutierrez, F., … Liberali, P. (2021). Cell fate coordinates mechano-osmotic forces in intestinal crypt formation. Nature Cell Biology. Springer Nature. https://doi.org/10.1038/s41556-021-00700-2","ama":"Yang Q, Xue S, Chan CJ, et al. Cell fate coordinates mechano-osmotic forces in intestinal crypt formation. Nature Cell Biology. 2021;23:733–744. doi:10.1038/s41556-021-00700-2","short":"Q. Yang, S. Xue, C.J. Chan, M. Rempfler, D. Vischi, F. Maurer-Gutierrez, T. Hiiragi, E.B. Hannezo, P. Liberali, Nature Cell Biology 23 (2021) 733–744.","ieee":"Q. Yang et al., “Cell fate coordinates mechano-osmotic forces in intestinal crypt formation,” Nature Cell Biology, vol. 23. Springer Nature, pp. 733–744, 2021.","chicago":"Yang, Qiutan, Shi-lei Xue, Chii Jou Chan, Markus Rempfler, Dario Vischi, Francisca Maurer-Gutierrez, Takashi Hiiragi, Edouard B Hannezo, and Prisca Liberali. “Cell Fate Coordinates Mechano-Osmotic Forces in Intestinal Crypt Formation.” Nature Cell Biology. Springer Nature, 2021. https://doi.org/10.1038/s41556-021-00700-2.","ista":"Yang Q, Xue S, Chan CJ, Rempfler M, Vischi D, Maurer-Gutierrez F, Hiiragi T, Hannezo EB, Liberali P. 2021. Cell fate coordinates mechano-osmotic forces in intestinal crypt formation. Nature Cell Biology. 23, 733–744."},"title":"Cell fate coordinates mechano-osmotic forces in intestinal crypt formation","author":[{"first_name":"Qiutan","full_name":"Yang, Qiutan","last_name":"Yang"},{"full_name":"Xue, Shi-lei","last_name":"Xue","id":"31D2C804-F248-11E8-B48F-1D18A9856A87","first_name":"Shi-lei"},{"last_name":"Chan","full_name":"Chan, Chii Jou","first_name":"Chii Jou"},{"first_name":"Markus","full_name":"Rempfler, Markus","last_name":"Rempfler"},{"last_name":"Vischi","full_name":"Vischi, Dario","first_name":"Dario"},{"last_name":"Maurer-Gutierrez","full_name":"Maurer-Gutierrez, Francisca","first_name":"Francisca"},{"last_name":"Hiiragi","full_name":"Hiiragi, Takashi","first_name":"Takashi"},{"first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B"},{"first_name":"Prisca","full_name":"Liberali, Prisca","last_name":"Liberali"}],"external_id":{"pmid":["34155381"],"isi":["000664016300003"]},"article_processing_charge":"No","project":[{"name":"Design Principles of Branching Morphogenesis","grant_number":"851288","_id":"05943252-7A3F-11EA-A408-12923DDC885E","call_identifier":"H2020"},{"name":"Active mechano-chemical description of the cell cytoskeleton","grant_number":"P31639","call_identifier":"FWF","_id":"268294B6-B435-11E9-9278-68D0E5697425"}],"language":[{"iso":"eng"}],"publication_identifier":{"eissn":["1476-4679"],"issn":["1465-7392"]},"publication_status":"published","volume":23,"ec_funded":1,"oa_version":"Preprint","pmid":1,"abstract":[{"text":"Intestinal organoids derived from single cells undergo complex crypt–villus patterning and morphogenesis. However, the nature and coordination of the underlying forces remains poorly characterized. Here, using light-sheet microscopy and large-scale imaging quantification, we demonstrate that crypt formation coincides with a stark reduction in lumen volume. We develop a 3D biophysical model to computationally screen different mechanical scenarios of crypt morphogenesis. Combining this with live-imaging data and multiple mechanical perturbations, we show that actomyosin-driven crypt apical contraction and villus basal tension work synergistically with lumen volume reduction to drive crypt morphogenesis, and demonstrate the existence of a critical point in differential tensions above which crypt morphology becomes robust to volume changes. Finally, we identified a sodium/glucose cotransporter that is specific to differentiated enterocytes that modulates lumen volume reduction through cell swelling in the villus region. Together, our study uncovers the cellular basis of how cell fate modulates osmotic and actomyosin forces to coordinate robust morphogenesis.","lang":"eng"}],"month":"06","intvolume":" 23","scopus_import":"1","main_file_link":[{"url":"https://www.biorxiv.org/content/10.1101/2020.05.13.094359","open_access":"1"}],"date_updated":"2023-08-10T13:57:36Z","department":[{"_id":"EdHa"}],"_id":"9629","status":"public","type":"journal_article","article_type":"original"},{"acknowledgement":"We would like to thank the entire Paluch and Baum laboratories at the MRC-LMCB and the Chalut lab at the Cambridge SCI for discussions and feedback throughout the project, and the MRC-LMCB microscopy platform, in particular Andrew Vaughan, for technical support.","publisher":"The Company of Biologists","quality_controlled":"1","oa":1,"day":"01","publication":"Journal of Cell Science","isi":1,"has_accepted_license":"1","year":"2021","date_published":"2021-07-01T00:00:00Z","doi":"10.1242/jcs.255018","date_created":"2021-08-22T22:01:20Z","article_number":"jcs255018","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"ieee":"A. Chaigne, M. B. Smith, R. L. Cavestany, E. B. Hannezo, K. J. Chalut, and E. K. Paluch, “Three-dimensional geometry controls division symmetry in stem cell colonies,” Journal of Cell Science, vol. 134, no. 14. The Company of Biologists, 2021.","short":"A. Chaigne, M.B. Smith, R.L. Cavestany, E.B. Hannezo, K.J. Chalut, E.K. Paluch, Journal of Cell Science 134 (2021).","apa":"Chaigne, A., Smith, M. B., Cavestany, R. L., Hannezo, E. B., Chalut, K. J., & Paluch, E. K. (2021). Three-dimensional geometry controls division symmetry in stem cell colonies. Journal of Cell Science. The Company of Biologists. https://doi.org/10.1242/jcs.255018","ama":"Chaigne A, Smith MB, Cavestany RL, Hannezo EB, Chalut KJ, Paluch EK. Three-dimensional geometry controls division symmetry in stem cell colonies. Journal of Cell Science. 2021;134(14). doi:10.1242/jcs.255018","mla":"Chaigne, Agathe, et al. “Three-Dimensional Geometry Controls Division Symmetry in Stem Cell Colonies.” Journal of Cell Science, vol. 134, no. 14, jcs255018, The Company of Biologists, 2021, doi:10.1242/jcs.255018.","ista":"Chaigne A, Smith MB, Cavestany RL, Hannezo EB, Chalut KJ, Paluch EK. 2021. Three-dimensional geometry controls division symmetry in stem cell colonies. Journal of Cell Science. 134(14), jcs255018.","chicago":"Chaigne, Agathe, Matthew B. Smith, R. L. Cavestany, Edouard B Hannezo, Kevin J. Chalut, and Ewa K. Paluch. “Three-Dimensional Geometry Controls Division Symmetry in Stem Cell Colonies.” Journal of Cell Science. The Company of Biologists, 2021. https://doi.org/10.1242/jcs.255018."},"title":"Three-dimensional geometry controls division symmetry in stem cell colonies","author":[{"last_name":"Chaigne","full_name":"Chaigne, Agathe","first_name":"Agathe"},{"full_name":"Smith, Matthew B.","last_name":"Smith","first_name":"Matthew B."},{"first_name":"R. L.","full_name":"Cavestany, R. L.","last_name":"Cavestany"},{"last_name":"Hannezo","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Chalut","full_name":"Chalut, Kevin J.","first_name":"Kevin J."},{"first_name":"Ewa K.","last_name":"Paluch","full_name":"Paluch, Ewa K."}],"article_processing_charge":"Yes (in subscription journal)","external_id":{"isi":["000681395800008"]},"oa_version":"Published Version","abstract":[{"lang":"eng","text":"Proper control of division orientation and symmetry, largely determined by spindle positioning, is essential to development and homeostasis. Spindle positioning has been extensively studied in cells dividing in two-dimensional (2D) environments and in epithelial tissues, where proteins such as NuMA (also known as NUMA1) orient division along the interphase long axis of the cell. However, little is known about how cells control spindle positioning in three-dimensional (3D) environments, such as early mammalian embryos and a variety of adult tissues. Here, we use mouse embryonic stem cells (ESCs), which grow in 3D colonies, as a model to investigate division in 3D. We observe that, at the periphery of 3D colonies, ESCs display high spindle mobility and divide asymmetrically. Our data suggest that enhanced spindle movements are due to unequal distribution of the cell–cell junction protein E-cadherin between future daughter cells. Interestingly, when cells progress towards differentiation, division becomes more symmetric, with more elongated shapes in metaphase and enhanced cortical NuMA recruitment in anaphase. Altogether, this study suggests that in 3D contexts, the geometry of the cell and its contacts with neighbors control division orientation and symmetry."}],"month":"07","intvolume":" 134","scopus_import":"1","file":[{"content_type":"application/pdf","access_level":"open_access","relation":"main_file","checksum":"f086f9d7cb63b2474c01921cb060c513","file_id":"9954","success":1,"date_updated":"2021-08-23T07:32:20Z","file_size":8651724,"creator":"asandaue","date_created":"2021-08-23T07:32:20Z","file_name":"2021_JournalOfCellScience_Chaigne.pdf"}],"language":[{"iso":"eng"}],"publication_identifier":{"eissn":["14779137"],"issn":["00219533"]},"publication_status":"published","volume":134,"issue":"14","_id":"9952","status":"public","type":"journal_article","article_type":"original","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"ddc":["570"],"date_updated":"2023-08-11T10:55:36Z","file_date_updated":"2021-08-23T07:32:20Z","department":[{"_id":"EdHa"}]},{"project":[{"grant_number":"851288","name":"Design Principles of Branching Morphogenesis","call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E"},{"name":"ISTplus - Postdoctoral Fellowships","grant_number":"754411","_id":"260C2330-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"}],"article_number":"6830","author":[{"last_name":"Ucar","full_name":"Ucar, Mehmet C","orcid":"0000-0003-0506-4217","first_name":"Mehmet C","id":"50B2A802-6007-11E9-A42B-EB23E6697425"},{"first_name":"Dmitrii","last_name":"Kamenev","full_name":"Kamenev, Dmitrii"},{"first_name":"Kazunori","full_name":"Sunadome, Kazunori","last_name":"Sunadome"},{"full_name":"Fachet, Dominik C","last_name":"Fachet","first_name":"Dominik C","id":"14FDD550-AA41-11E9-A0E5-1ACCE5697425"},{"full_name":"Lallemend, Francois","last_name":"Lallemend","first_name":"Francois"},{"last_name":"Adameyko","full_name":"Adameyko, Igor","first_name":"Igor"},{"last_name":"Hadjab","full_name":"Hadjab, Saida","first_name":"Saida"},{"first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561"}],"external_id":{"isi":["000722322900020"],"pmid":["34819507"]},"article_processing_charge":"No","title":"Theory of branching morphogenesis by local interactions and global guidance","citation":{"mla":"Ucar, Mehmet C., et al. “Theory of Branching Morphogenesis by Local Interactions and Global Guidance.” Nature Communications, vol. 12, 6830, Springer Nature, 2021, doi:10.1038/s41467-021-27135-5.","short":"M.C. Ucar, D. Kamenev, K. Sunadome, D.C. Fachet, F. Lallemend, I. Adameyko, S. Hadjab, E.B. Hannezo, Nature Communications 12 (2021).","ieee":"M. C. Ucar et al., “Theory of branching morphogenesis by local interactions and global guidance,” Nature Communications, vol. 12. Springer Nature, 2021.","apa":"Ucar, M. C., Kamenev, D., Sunadome, K., Fachet, D. C., Lallemend, F., Adameyko, I., … Hannezo, E. B. (2021). Theory of branching morphogenesis by local interactions and global guidance. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-021-27135-5","ama":"Ucar MC, Kamenev D, Sunadome K, et al. Theory of branching morphogenesis by local interactions and global guidance. Nature Communications. 2021;12. doi:10.1038/s41467-021-27135-5","chicago":"Ucar, Mehmet C, Dmitrii Kamenev, Kazunori Sunadome, Dominik C Fachet, Francois Lallemend, Igor Adameyko, Saida Hadjab, and Edouard B Hannezo. “Theory of Branching Morphogenesis by Local Interactions and Global Guidance.” Nature Communications. Springer Nature, 2021. https://doi.org/10.1038/s41467-021-27135-5.","ista":"Ucar MC, Kamenev D, Sunadome K, Fachet DC, Lallemend F, Adameyko I, Hadjab S, Hannezo EB. 2021. Theory of branching morphogenesis by local interactions and global guidance. Nature Communications. 12, 6830."},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","quality_controlled":"1","publisher":"Springer Nature","oa":1,"acknowledgement":"We thank all members of our respective groups for helpful discussion on the paper. The authors are also grateful to Prof. Abdel El. Manira for support and sharing Tg(HUC:Gal4;UAS:Synaptohysin-GFP), to Haohao Wu for discussion, and thank Elena Zabalueva for the zebrafish schematic. The authors also acknowledge Zebrafish core facility, Genome Engineering Zebrafish and Biomedicum Imaging Core from the Karolinska Institutet for technical support. This work received funding from the ERC under the European Union’s Horizon 2020 research and innovation programme (grant agreement No. 851288 to E.H.) and under the Marie Skłodowska-Curie grant agreement No. 754411 (to M.C.U.); Swedish Research Council (to F.L., I.A. and S.H.); Knut and Alice Wallenberg Foundation (F.L. and I.A.); Swedish Brain Foundation (F.L. and S.H.); Ming Wai Lau Foundation (to F.L.); StratRegen (to F.L.); ERC Consolidator grant STEMMING-FROM-NERVE and ERC Synergy Grant KILL-OR-DIFFERENTIATE (to I.A.); Bertil Hallsten Research Foundation (to I.A.); Cancerfonden (to I.A.); the Paradifference Foundation (to I.A.); Austrian Science Fund (to I.A.); and StratNeuro (to S.H.).","date_published":"2021-11-24T00:00:00Z","doi":"10.1038/s41467-021-27135-5","date_created":"2021-12-05T23:01:40Z","isi":1,"has_accepted_license":"1","year":"2021","day":"24","publication":"Nature Communications","article_type":"original","type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"status":"public","_id":"10402","file_date_updated":"2021-12-10T08:54:09Z","department":[{"_id":"EdHa"}],"date_updated":"2023-08-14T13:18:46Z","ddc":["573"],"scopus_import":"1","month":"11","intvolume":" 12","abstract":[{"lang":"eng","text":"Branching morphogenesis governs the formation of many organs such as lung, kidney, and the neurovascular system. Many studies have explored system-specific molecular and cellular regulatory mechanisms, as well as self-organizing rules underlying branching morphogenesis. However, in addition to local cues, branched tissue growth can also be influenced by global guidance. Here, we develop a theoretical framework for a stochastic self-organized branching process in the presence of external cues. Combining analytical theory with numerical simulations, we predict differential signatures of global vs. local regulatory mechanisms on the branching pattern, such as angle distributions, domain size, and space-filling efficiency. We find that branch alignment follows a generic scaling law determined by the strength of global guidance, while local interactions influence the tissue density but not its overall territory. Finally, using zebrafish innervation as a model system, we test these key features of the model experimentally. Our work thus provides quantitative predictions to disentangle the role of different types of cues in shaping branched structures across scales."}],"pmid":1,"oa_version":"Published Version","volume":12,"related_material":{"record":[{"relation":"research_data","id":"13058","status":"public"}]},"ec_funded":1,"publication_identifier":{"eissn":["2041-1723"]},"publication_status":"published","file":[{"checksum":"63c56ec75314a71e63e7dd2920b3c5b5","file_id":"10529","success":1,"access_level":"open_access","relation":"main_file","content_type":"application/pdf","date_created":"2021-12-10T08:54:09Z","file_name":"2021_NatComm_Ucar.pdf","creator":"cchlebak","date_updated":"2021-12-10T08:54:09Z","file_size":2303405}],"language":[{"iso":"eng"}]},{"_id":"10573","status":"public","type":"journal_article","article_type":"original","date_updated":"2023-08-17T06:28:25Z","department":[{"_id":"EdHa"}],"oa_version":"Preprint","abstract":[{"text":"How tissues acquire complex shapes is a fundamental question in biology and regenerative medicine. Zebrafish semicircular canals form from invaginations in the otic epithelium (buds) that extend and fuse to form the hubs of each canal. We find that conventional actomyosin-driven behaviors are not required. Instead, local secretion of hyaluronan, made by the enzymes uridine 5′-diphosphate dehydrogenase (ugdh) and hyaluronan synthase 3 (has3), drives canal morphogenesis. Charged hyaluronate polymers osmotically swell with water and generate isotropic extracellular pressure to deform the overlying epithelium into buds. The mechanical anisotropy needed to shape buds into tubes is conferred by a polarized distribution of actomyosin and E-cadherin-rich membrane tethers, which we term cytocinches. Most work on tissue morphogenesis ascribes actomyosin contractility as the driving force, while the extracellular matrix shapes tissues through differential stiffness. Our work inverts this expectation. Hyaluronate pressure shaped by anisotropic tissue stiffness may be a widespread mechanism for powering morphological change in organogenesis and tissue engineering.","lang":"eng"}],"intvolume":" 184","month":"12","main_file_link":[{"url":"https://www.biorxiv.org/content/10.1101/2020.09.28.316042","open_access":"1"}],"scopus_import":"1","language":[{"iso":"eng"}],"publication_status":"published","publication_identifier":{"eissn":["1097-4172"],"issn":["0092-8674"]},"volume":184,"issue":"26","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"ista":"Munjal A, Hannezo EB, Tsai TYC, Mitchison TJ, Megason SG. 2021. Extracellular hyaluronate pressure shaped by cellular tethers drives tissue morphogenesis. Cell. 184(26), 6313–6325.e18.","chicago":"Munjal, Akankshi, Edouard B Hannezo, Tony Y.C. Tsai, Timothy J. Mitchison, and Sean G. Megason. “Extracellular Hyaluronate Pressure Shaped by Cellular Tethers Drives Tissue Morphogenesis.” Cell. Elsevier ; Cell Press, 2021. https://doi.org/10.1016/j.cell.2021.11.025.","apa":"Munjal, A., Hannezo, E. B., Tsai, T. Y. C., Mitchison, T. J., & Megason, S. G. (2021). Extracellular hyaluronate pressure shaped by cellular tethers drives tissue morphogenesis. Cell. Elsevier ; Cell Press. https://doi.org/10.1016/j.cell.2021.11.025","ama":"Munjal A, Hannezo EB, Tsai TYC, Mitchison TJ, Megason SG. Extracellular hyaluronate pressure shaped by cellular tethers drives tissue morphogenesis. Cell. 2021;184(26):6313-6325.e18. doi:10.1016/j.cell.2021.11.025","short":"A. Munjal, E.B. Hannezo, T.Y.C. Tsai, T.J. Mitchison, S.G. Megason, Cell 184 (2021) 6313–6325.e18.","ieee":"A. Munjal, E. B. Hannezo, T. Y. C. Tsai, T. J. Mitchison, and S. G. Megason, “Extracellular hyaluronate pressure shaped by cellular tethers drives tissue morphogenesis,” Cell, vol. 184, no. 26. Elsevier ; Cell Press, p. 6313–6325.e18, 2021.","mla":"Munjal, Akankshi, et al. “Extracellular Hyaluronate Pressure Shaped by Cellular Tethers Drives Tissue Morphogenesis.” Cell, vol. 184, no. 26, Elsevier ; Cell Press, 2021, p. 6313–6325.e18, doi:10.1016/j.cell.2021.11.025."},"title":"Extracellular hyaluronate pressure shaped by cellular tethers drives tissue morphogenesis","article_processing_charge":"No","external_id":{"isi":["000735387500002"]},"author":[{"first_name":"Akankshi","full_name":"Munjal, Akankshi","last_name":"Munjal"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561"},{"full_name":"Tsai, Tony Y.C.","last_name":"Tsai","first_name":"Tony Y.C."},{"full_name":"Mitchison, Timothy J.","last_name":"Mitchison","first_name":"Timothy J."},{"full_name":"Megason, Sean G.","last_name":"Megason","first_name":"Sean G."}],"acknowledgement":"We thank Ian Swinburne, Sandy Nandagopal, and Toru Kawanishi for support, discussions, and reagents. We thank Vanessa Barone, Joseph Nasser, and members of the Megason lab for useful comments on the manuscript and general feedback. We are grateful to the Heisenberg and Knaut labs for transgenic fish. Diagrams on the right in the graphical abstract were created using BioRender. This work was supported by NIH R01DC015478 and NIH R01GM107733 to S.G.M. A.M. was supported by Human Frontiers Science Program LTF and NIH K99HD098918.","oa":1,"quality_controlled":"1","publisher":"Elsevier ; Cell Press","publication":"Cell","day":"22","year":"2021","isi":1,"date_created":"2021-12-26T23:01:26Z","doi":"10.1016/j.cell.2021.11.025","date_published":"2021-12-22T00:00:00Z","page":"6313-6325.e18"},{"citation":{"chicago":"Luciano, Marine, Shi-lei Xue, Winnok H. De Vos, Lorena Redondo-Morata, Mathieu Surin, Frank Lafont, Edouard B Hannezo, and Sylvain Gabriele. “Cell Monolayers Sense Curvature by Exploiting Active Mechanics and Nuclear Mechanoadaptation.” Nature Physics. Springer Nature, 2021. https://doi.org/10.1038/s41567-021-01374-1.","ista":"Luciano M, Xue S, De Vos WH, Redondo-Morata L, Surin M, Lafont F, Hannezo EB, Gabriele S. 2021. Cell monolayers sense curvature by exploiting active mechanics and nuclear mechanoadaptation. Nature Physics. 17(12), 1382–1390.","mla":"Luciano, Marine, et al. “Cell Monolayers Sense Curvature by Exploiting Active Mechanics and Nuclear Mechanoadaptation.” Nature Physics, vol. 17, no. 12, Springer Nature, 2021, pp. 1382–1390, doi:10.1038/s41567-021-01374-1.","short":"M. Luciano, S. Xue, W.H. De Vos, L. Redondo-Morata, M. Surin, F. Lafont, E.B. Hannezo, S. Gabriele, Nature Physics 17 (2021) 1382–1390.","ieee":"M. Luciano et al., “Cell monolayers sense curvature by exploiting active mechanics and nuclear mechanoadaptation,” Nature Physics, vol. 17, no. 12. Springer Nature, pp. 1382–1390, 2021.","ama":"Luciano M, Xue S, De Vos WH, et al. Cell monolayers sense curvature by exploiting active mechanics and nuclear mechanoadaptation. Nature Physics. 2021;17(12):1382–1390. doi:10.1038/s41567-021-01374-1","apa":"Luciano, M., Xue, S., De Vos, W. H., Redondo-Morata, L., Surin, M., Lafont, F., … Gabriele, S. (2021). Cell monolayers sense curvature by exploiting active mechanics and nuclear mechanoadaptation. Nature Physics. Springer Nature. https://doi.org/10.1038/s41567-021-01374-1"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","external_id":{"isi":["000720204300004"]},"article_processing_charge":"No","author":[{"first_name":"Marine","last_name":"Luciano","full_name":"Luciano, Marine"},{"last_name":"Xue","full_name":"Xue, Shi-lei","id":"31D2C804-F248-11E8-B48F-1D18A9856A87","first_name":"Shi-lei"},{"last_name":"De Vos","full_name":"De Vos, Winnok H.","first_name":"Winnok H."},{"last_name":"Redondo-Morata","full_name":"Redondo-Morata, Lorena","first_name":"Lorena"},{"first_name":"Mathieu","last_name":"Surin","full_name":"Surin, Mathieu"},{"first_name":"Frank","last_name":"Lafont","full_name":"Lafont, Frank"},{"orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","last_name":"Hannezo","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Gabriele, Sylvain","last_name":"Gabriele","first_name":"Sylvain"}],"title":"Cell monolayers sense curvature by exploiting active mechanics and nuclear mechanoadaptation","project":[{"call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E","name":"Design Principles of Branching Morphogenesis","grant_number":"851288"},{"call_identifier":"FWF","_id":"268294B6-B435-11E9-9278-68D0E5697425","name":"Active mechano-chemical description of the cell cytoskeleton","grant_number":"P31639"}],"year":"2021","has_accepted_license":"1","isi":1,"publication":"Nature Physics","day":"18","page":"1382–1390","date_created":"2021-11-28T23:01:29Z","date_published":"2021-11-18T00:00:00Z","doi":"10.1038/s41567-021-01374-1","acknowledgement":"S.G. acknowledges funding from FEDER Prostem Research Project no. 1510614 (Wallonia DG06), F.R.S.-FNRS Epiforce Research Project no. T.0092.21 and Interreg MAT(T)ISSE project, which is financially supported by Interreg France-Wallonie-Vlaanderen (Fonds Européen de Développement Régional, FEDER-ERDF). This project was supported by the European Research Council under the European Union’s Horizon 2020 Research and Innovation Programme grant agreement 851288 (to E.H.), and by the Austrian Science Fund (FWF) (P 31639; to E.H.). L.R.M. acknowledges funding from the Agence National de la Recherche (ANR), as part of the ‘Investments d’Avenir’ Programme (I-SITE ULNE/ANR-16-IDEX-0004 ULNE). This work benefited from ANR-10-EQPX-04-01 and FEDER 12001407 grants to F.L. W.D.V. is supported by the Research Foundation Flanders (FWO 1516619N, FWO GOO5819N, FWO I003420N, FWO IRI I000321N) and is member of the Research Excellence Consortium µNEURO at the University of Antwerp. M.L. is financially supported by FRIA (F.R.S.-FNRS). M.S. is a Senior Research Associate of the Fund for Scientific Research (F.R.S.-FNRS) and acknowledges EOS grant no. 30650939 (PRECISION). Sketches in Figs. 1a and 5e and Extended Data Fig. 9 were drawn by C. Levicek.","oa":1,"quality_controlled":"1","publisher":"Springer Nature","date_updated":"2023-10-16T06:31:54Z","ddc":["530"],"file_date_updated":"2023-10-11T09:31:43Z","department":[{"_id":"EdHa"}],"_id":"10365","article_type":"original","type":"journal_article","status":"public","publication_status":"published","publication_identifier":{"eissn":["1745-2481"],"issn":["1745-2473"]},"language":[{"iso":"eng"}],"file":[{"date_updated":"2023-10-11T09:31:43Z","file_size":40285498,"creator":"channezo","date_created":"2023-10-11T09:31:43Z","file_name":"50145_4_merged_1630498627.pdf","content_type":"application/pdf","access_level":"open_access","relation":"main_file","file_id":"14420","checksum":"5d6d76750a71d7cb632bb15417c38ef7","success":1}],"ec_funded":1,"issue":"12","related_material":{"link":[{"relation":"press_release","url":"https://ist.ac.at/en/news/how-cells-feel-curvature/","description":"News on IST Webpage"}]},"volume":17,"abstract":[{"text":"The early development of many organisms involves the folding of cell monolayers, but this behaviour is difficult to reproduce in vitro; therefore, both mechanistic causes and effects of local curvature remain unclear. Here we study epithelial cell monolayers on corrugated hydrogels engineered into wavy patterns, examining how concave and convex curvatures affect cellular and nuclear shape. We find that substrate curvature affects monolayer thickness, which is larger in valleys than crests. We show that this feature generically arises in a vertex model, leading to the hypothesis that cells may sense curvature by modifying the thickness of the tissue. We find that local curvature also affects nuclear morphology and positioning, which we explain by extending the vertex model to take into account membrane–nucleus interactions, encoding thickness modulation in changes to nuclear deformation and position. We propose that curvature governs the spatial distribution of yes-associated proteins via nuclear shape and density changes. We show that curvature also induces significant variations in lamins, chromatin condensation and cell proliferation rate in folded epithelial tissues. Together, this work identifies active cell mechanics and nuclear mechanoadaptation as the key players of the mechanistic regulation of epithelia to substrate curvature.","lang":"eng"}],"oa_version":"Submitted Version","scopus_import":"1","intvolume":" 17","month":"11"},{"day":"30","publication":"Cell","isi":1,"has_accepted_license":"1","year":"2020","doi":"10.1016/j.cell.2020.03.015","date_published":"2020-04-30T00:00:00Z","date_created":"2020-05-03T22:00:48Z","page":"604-620.e22","publisher":"Elsevier","quality_controlled":"1","oa":1,"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"chicago":"Dekoninck, Sophie, Edouard B Hannezo, Alejandro Sifrim, Yekaterina A. Miroshnikova, Mariaceleste Aragona, Milan Malfait, Souhir Gargouri, et al. “Defining the Design Principles of Skin Epidermis Postnatal Growth.” Cell. Elsevier, 2020. https://doi.org/10.1016/j.cell.2020.03.015.","ista":"Dekoninck S, Hannezo EB, Sifrim A, Miroshnikova YA, Aragona M, Malfait M, Gargouri S, De Neunheuser C, Dubois C, Voet T, Wickström SA, Simons BD, Blanpain C. 2020. Defining the design principles of skin epidermis postnatal growth. Cell. 181(3), 604–620.e22.","mla":"Dekoninck, Sophie, et al. “Defining the Design Principles of Skin Epidermis Postnatal Growth.” Cell, vol. 181, no. 3, Elsevier, 2020, p. 604–620.e22, doi:10.1016/j.cell.2020.03.015.","apa":"Dekoninck, S., Hannezo, E. B., Sifrim, A., Miroshnikova, Y. A., Aragona, M., Malfait, M., … Blanpain, C. (2020). Defining the design principles of skin epidermis postnatal growth. Cell. Elsevier. https://doi.org/10.1016/j.cell.2020.03.015","ama":"Dekoninck S, Hannezo EB, Sifrim A, et al. Defining the design principles of skin epidermis postnatal growth. Cell. 2020;181(3):604-620.e22. doi:10.1016/j.cell.2020.03.015","short":"S. Dekoninck, E.B. Hannezo, A. Sifrim, Y.A. Miroshnikova, M. Aragona, M. Malfait, S. Gargouri, C. De Neunheuser, C. Dubois, T. Voet, S.A. Wickström, B.D. Simons, C. Blanpain, Cell 181 (2020) 604–620.e22.","ieee":"S. Dekoninck et al., “Defining the design principles of skin epidermis postnatal growth,” Cell, vol. 181, no. 3. Elsevier, p. 604–620.e22, 2020."},"title":"Defining the design principles of skin epidermis postnatal growth","author":[{"first_name":"Sophie","full_name":"Dekoninck, Sophie","last_name":"Dekoninck"},{"first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561"},{"first_name":"Alejandro","last_name":"Sifrim","full_name":"Sifrim, Alejandro"},{"full_name":"Miroshnikova, Yekaterina A.","last_name":"Miroshnikova","first_name":"Yekaterina A."},{"full_name":"Aragona, Mariaceleste","last_name":"Aragona","first_name":"Mariaceleste"},{"first_name":"Milan","full_name":"Malfait, Milan","last_name":"Malfait"},{"first_name":"Souhir","full_name":"Gargouri, Souhir","last_name":"Gargouri"},{"full_name":"De Neunheuser, Charlotte","last_name":"De Neunheuser","first_name":"Charlotte"},{"last_name":"Dubois","full_name":"Dubois, Christine","first_name":"Christine"},{"full_name":"Voet, Thierry","last_name":"Voet","first_name":"Thierry"},{"last_name":"Wickström","full_name":"Wickström, Sara A.","first_name":"Sara A."},{"last_name":"Simons","full_name":"Simons, Benjamin D.","first_name":"Benjamin D."},{"first_name":"Cédric","last_name":"Blanpain","full_name":"Blanpain, Cédric"}],"external_id":{"pmid":["32259486"],"isi":["000530708400016"]},"article_processing_charge":"No","file":[{"date_created":"2020-05-04T10:20:55Z","file_name":"2020_Cell_Dekoninck.pdf","date_updated":"2020-07-14T12:48:03Z","file_size":17992888,"creator":"dernst","checksum":"e2114902f4e9d75a752e9efb5ae06011","file_id":"7795","content_type":"application/pdf","access_level":"open_access","relation":"main_file"}],"language":[{"iso":"eng"}],"publication_identifier":{"issn":["00928674"],"eissn":["10974172"]},"publication_status":"published","volume":181,"issue":"3","pmid":1,"oa_version":"Published Version","abstract":[{"lang":"eng","text":"During embryonic and postnatal development, organs and tissues grow steadily to achieve their final size at the end of puberty. However, little is known about the cellular dynamics that mediate postnatal growth. By combining in vivo clonal lineage tracing, proliferation kinetics, single-cell transcriptomics, andin vitro micro-pattern experiments, we resolved the cellular dynamics taking place during postnatal skin epidermis expansion. Our data revealed that harmonious growth is engineered by a single population of developmental progenitors presenting a fixed fate imbalance of self-renewing divisions with an ever-decreasing proliferation rate. Single-cell RNA sequencing revealed that epidermal developmental progenitors form a more uniform population compared with adult stem and progenitor cells. Finally, we found that the spatial pattern of cell division orientation is dictated locally by the underlying collagen fiber orientation. Our results uncover a simple design principle of organ growth where progenitors and differentiated cells expand in harmony with their surrounding tissues."}],"month":"04","intvolume":" 181","scopus_import":"1","ddc":["570"],"date_updated":"2023-08-21T06:17:43Z","department":[{"_id":"EdHa"}],"file_date_updated":"2020-07-14T12:48:03Z","_id":"7789","status":"public","article_type":"original","type":"journal_article","tmp":{"short":"CC BY-NC-ND (4.0)","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png"}},{"date_updated":"2023-08-22T08:29:30Z","ddc":["570"],"department":[{"_id":"EdHa"}],"file_date_updated":"2020-08-10T06:50:28Z","_id":"8220","type":"journal_article","article_type":"original","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"status":"public","publication_identifier":{"eissn":["10916490"]},"publication_status":"published","file":[{"success":1,"file_id":"8223","relation":"main_file","access_level":"open_access","content_type":"application/pdf","file_name":"2020_PNAS_Corominas.pdf","date_created":"2020-08-10T06:50:28Z","creator":"dernst","file_size":1111604,"date_updated":"2020-08-10T06:50:28Z"}],"language":[{"iso":"eng"}],"issue":"29","related_material":{"link":[{"relation":"press_release","url":"https://ist.ac.at/en/news/order-from-noise/"}]},"volume":117,"ec_funded":1,"abstract":[{"lang":"eng","text":"Understanding to what extent stem cell potential is a cell-intrinsic property or an emergent behavior coming from global tissue dynamics and geometry is a key outstanding question of systems and stem cell biology. Here, we propose a theory of stem cell dynamics as a stochastic competition for access to a spatially localized niche, giving rise to a stochastic conveyor-belt model. Cell divisions produce a steady cellular stream which advects cells away from the niche, while random rearrangements enable cells away from the niche to be favorably repositioned. Importantly, even when assuming that all cells in a tissue are molecularly equivalent, we predict a common (“universal”) functional dependence of the long-term clonal survival probability on distance from the niche, as well as the emergence of a well-defined number of functional stem cells, dependent only on the rate of random movements vs. mitosis-driven advection. We test the predictions of this theory on datasets of pubertal mammary gland tips and embryonic kidney tips, as well as homeostatic intestinal crypts. Importantly, we find good agreement for the predicted functional dependency of the competition as a function of position, and thus functional stem cell number in each organ. This argues for a key role of positional fluctuations in dictating stem cell number and dynamics, and we discuss the applicability of this theory to other settings."}],"pmid":1,"oa_version":"Published Version","scopus_import":"1","month":"07","intvolume":" 117","citation":{"ista":"Corominas-Murtra B, Scheele CLGJ, Kishi K, Ellenbroek SIJ, Simons BD, Van Rheenen J, Hannezo EB. 2020. Stem cell lineage survival as a noisy competition for niche access. Proceedings of the National Academy of Sciences of the United States of America. 117(29), 16969–16975.","chicago":"Corominas-Murtra, Bernat, Colinda L.G.J. Scheele, Kasumi Kishi, Saskia I.J. Ellenbroek, Benjamin D. Simons, Jacco Van Rheenen, and Edouard B Hannezo. “Stem Cell Lineage Survival as a Noisy Competition for Niche Access.” Proceedings of the National Academy of Sciences of the United States of America. National Academy of Sciences, 2020. https://doi.org/10.1073/pnas.1921205117.","apa":"Corominas-Murtra, B., Scheele, C. L. G. J., Kishi, K., Ellenbroek, S. I. J., Simons, B. D., Van Rheenen, J., & Hannezo, E. B. (2020). Stem cell lineage survival as a noisy competition for niche access. Proceedings of the National Academy of Sciences of the United States of America. National Academy of Sciences. https://doi.org/10.1073/pnas.1921205117","ama":"Corominas-Murtra B, Scheele CLGJ, Kishi K, et al. Stem cell lineage survival as a noisy competition for niche access. Proceedings of the National Academy of Sciences of the United States of America. 2020;117(29):16969-16975. doi:10.1073/pnas.1921205117","ieee":"B. Corominas-Murtra et al., “Stem cell lineage survival as a noisy competition for niche access,” Proceedings of the National Academy of Sciences of the United States of America, vol. 117, no. 29. National Academy of Sciences, pp. 16969–16975, 2020.","short":"B. Corominas-Murtra, C.L.G.J. Scheele, K. Kishi, S.I.J. Ellenbroek, B.D. Simons, J. Van Rheenen, E.B. Hannezo, Proceedings of the National Academy of Sciences of the United States of America 117 (2020) 16969–16975.","mla":"Corominas-Murtra, Bernat, et al. “Stem Cell Lineage Survival as a Noisy Competition for Niche Access.” Proceedings of the National Academy of Sciences of the United States of America, vol. 117, no. 29, National Academy of Sciences, 2020, pp. 16969–75, doi:10.1073/pnas.1921205117."},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","author":[{"last_name":"Corominas-Murtra","orcid":"0000-0001-9806-5643","full_name":"Corominas-Murtra, Bernat","first_name":"Bernat","id":"43BE2298-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Scheele","full_name":"Scheele, Colinda L.G.J.","first_name":"Colinda L.G.J."},{"first_name":"Kasumi","id":"3065DFC4-F248-11E8-B48F-1D18A9856A87","full_name":"Kishi, Kasumi","last_name":"Kishi"},{"last_name":"Ellenbroek","full_name":"Ellenbroek, Saskia I.J.","first_name":"Saskia I.J."},{"first_name":"Benjamin D.","full_name":"Simons, Benjamin D.","last_name":"Simons"},{"last_name":"Van Rheenen","full_name":"Van Rheenen, Jacco","first_name":"Jacco"},{"last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"}],"external_id":{"pmid":["32611816"],"isi":["000553292900014"]},"article_processing_charge":"No","title":"Stem cell lineage survival as a noisy competition for niche access","project":[{"name":"Design Principles of Branching Morphogenesis","grant_number":"851288","_id":"05943252-7A3F-11EA-A408-12923DDC885E","call_identifier":"H2020"}],"isi":1,"has_accepted_license":"1","year":"2020","day":"21","publication":"Proceedings of the National Academy of Sciences of the United States of America","page":"16969-16975","date_published":"2020-07-21T00:00:00Z","doi":"10.1073/pnas.1921205117","date_created":"2020-08-09T22:00:52Z","acknowledgement":"We thank all members of the E.H., B.D.S., and J.v.R. groups for stimulating discussions. This project was supported by\r\nthe European Research Council (648804 to J.v.R. and 851288 to E.H.). It has also received support from the CancerGenomics.nl (Netherlands Organization for Scientific Research) program (J.v.R.) and the Doctor Josef Steiner Foundation (J.v.R). B.D.S. was supported by Royal Society E. P. Abraham Research Professorship RP/R1/180165 and Wellcome Trust Grant 098357/Z/12/Z.","publisher":"National Academy of Sciences","quality_controlled":"1","oa":1},{"article_number":"5037","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"mla":"Sznurkowska, Magdalena K., et al. “Tracing the Cellular Basis of Islet Specification in Mouse Pancreas.” Nature Communications, vol. 11, 5037, Springer Nature, 2020, doi:10.1038/s41467-020-18837-3.","ieee":"M. K. Sznurkowska et al., “Tracing the cellular basis of islet specification in mouse pancreas,” Nature Communications, vol. 11. Springer Nature, 2020.","short":"M.K. Sznurkowska, E.B. Hannezo, R. Azzarelli, L. Chatzeli, T. Ikeda, S. Yoshida, A. Philpott, B.D. Simons, Nature Communications 11 (2020).","ama":"Sznurkowska MK, Hannezo EB, Azzarelli R, et al. Tracing the cellular basis of islet specification in mouse pancreas. Nature Communications. 2020;11. doi:10.1038/s41467-020-18837-3","apa":"Sznurkowska, M. K., Hannezo, E. B., Azzarelli, R., Chatzeli, L., Ikeda, T., Yoshida, S., … Simons, B. D. (2020). Tracing the cellular basis of islet specification in mouse pancreas. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-020-18837-3","chicago":"Sznurkowska, Magdalena K., Edouard B Hannezo, Roberta Azzarelli, Lemonia Chatzeli, Tatsuro Ikeda, Shosei Yoshida, Anna Philpott, and Benjamin D Simons. “Tracing the Cellular Basis of Islet Specification in Mouse Pancreas.” Nature Communications. Springer Nature, 2020. https://doi.org/10.1038/s41467-020-18837-3.","ista":"Sznurkowska MK, Hannezo EB, Azzarelli R, Chatzeli L, Ikeda T, Yoshida S, Philpott A, Simons BD. 2020. Tracing the cellular basis of islet specification in mouse pancreas. Nature Communications. 11, 5037."},"title":"Tracing the cellular basis of islet specification in mouse pancreas","author":[{"full_name":"Sznurkowska, Magdalena K.","last_name":"Sznurkowska","first_name":"Magdalena K."},{"orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"},{"first_name":"Roberta","full_name":"Azzarelli, Roberta","last_name":"Azzarelli"},{"first_name":"Lemonia","last_name":"Chatzeli","full_name":"Chatzeli, Lemonia"},{"first_name":"Tatsuro","full_name":"Ikeda, Tatsuro","last_name":"Ikeda"},{"first_name":"Shosei","full_name":"Yoshida, Shosei","last_name":"Yoshida"},{"full_name":"Philpott, Anna","last_name":"Philpott","first_name":"Anna"},{"first_name":"Benjamin D","last_name":"Simons","full_name":"Simons, Benjamin D"}],"article_processing_charge":"No","external_id":{"isi":["000577244600003"],"pmid":["33028844"]},"publisher":"Springer Nature","quality_controlled":"1","oa":1,"day":"07","publication":"Nature Communications","has_accepted_license":"1","isi":1,"year":"2020","date_published":"2020-10-07T00:00:00Z","doi":"10.1038/s41467-020-18837-3","date_created":"2020-10-18T22:01:35Z","_id":"8669","status":"public","type":"journal_article","article_type":"original","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"ddc":["570"],"date_updated":"2023-08-22T10:18:17Z","file_date_updated":"2020-10-19T11:27:46Z","department":[{"_id":"EdHa"}],"oa_version":"Published Version","pmid":1,"abstract":[{"lang":"eng","text":"Pancreatic islets play an essential role in regulating blood glucose level. Although the molecular pathways underlying islet cell differentiation are beginning to be resolved, the cellular basis of islet morphogenesis and fate allocation remain unclear. By combining unbiased and targeted lineage tracing, we address the events leading to islet formation in the mouse. From the statistical analysis of clones induced at multiple embryonic timepoints, here we show that, during the secondary transition, islet formation involves the aggregation of multiple equipotent endocrine progenitors that transition from a phase of stochastic amplification by cell division into a phase of sublineage restriction and limited islet fission. Together, these results explain quantitatively the heterogeneous size distribution and degree of polyclonality of maturing islets, as well as dispersion of progenitors within and between islets. Further, our results show that, during the secondary transition, α- and β-cells are generated in a contemporary manner. Together, these findings provide insight into the cellular basis of islet development."}],"month":"10","intvolume":" 11","scopus_import":"1","file":[{"date_updated":"2020-10-19T11:27:46Z","file_size":5540540,"creator":"dernst","date_created":"2020-10-19T11:27:46Z","file_name":"2020_NatureComm_Sznurkowska.pdf","content_type":"application/pdf","access_level":"open_access","relation":"main_file","file_id":"8677","checksum":"0ecc0eab72d2d50694852579611a6624","success":1}],"language":[{"iso":"eng"}],"publication_identifier":{"eissn":["20411723"]},"publication_status":"published","volume":11},{"date_created":"2020-10-18T22:01:37Z","date_published":"2020-10-26T00:00:00Z","doi":"10.1016/j.devcel.2020.09.001","page":"195-208","publication":"Developmental Cell","day":"26","year":"2020","has_accepted_license":"1","isi":1,"oa":1,"quality_controlled":"1","publisher":"Elsevier","acknowledgement":"This work was supported by the Medical Research Council UK (MRC Program award MC_UU_12018/5 ), the European Research Council (starting grant 311637 -MorphoCorDiv and consolidator grant 820188 -NanoMechShape to E.K.P.), and the Leverhulme Trust (Leverhulme Prize in Biological Sciences to E.K.P.). K.J.C. acknowledges support from the Royal Society (Royal Society Research Fellowship). A.C. acknowledges support from EMBO ( ALTF 2015-563 ), the Wellcome Trust ( 201334/Z/16/Z ), and the Fondation Bettencourt-Schueller (Prix Jeune Chercheur, 2015).","title":"Abscission couples cell division to embryonic stem cell fate","external_id":{"isi":["000582501100012"],"pmid":["32979313"]},"article_processing_charge":"No","author":[{"full_name":"Chaigne, Agathe","last_name":"Chaigne","first_name":"Agathe"},{"full_name":"Labouesse, Céline","last_name":"Labouesse","first_name":"Céline"},{"last_name":"White","full_name":"White, Ian J.","first_name":"Ian J."},{"first_name":"Meghan","full_name":"Agnew, Meghan","last_name":"Agnew"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","last_name":"Hannezo"},{"first_name":"Kevin J.","full_name":"Chalut, Kevin J.","last_name":"Chalut"},{"first_name":"Ewa K.","last_name":"Paluch","full_name":"Paluch, Ewa K."}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"short":"A. Chaigne, C. Labouesse, I.J. White, M. Agnew, E.B. Hannezo, K.J. Chalut, E.K. Paluch, Developmental Cell 55 (2020) 195–208.","ieee":"A. Chaigne et al., “Abscission couples cell division to embryonic stem cell fate,” Developmental Cell, vol. 55, no. 2. Elsevier, pp. 195–208, 2020.","apa":"Chaigne, A., Labouesse, C., White, I. J., Agnew, M., Hannezo, E. B., Chalut, K. J., & Paluch, E. K. (2020). Abscission couples cell division to embryonic stem cell fate. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2020.09.001","ama":"Chaigne A, Labouesse C, White IJ, et al. Abscission couples cell division to embryonic stem cell fate. Developmental Cell. 2020;55(2):195-208. doi:10.1016/j.devcel.2020.09.001","mla":"Chaigne, Agathe, et al. “Abscission Couples Cell Division to Embryonic Stem Cell Fate.” Developmental Cell, vol. 55, no. 2, Elsevier, 2020, pp. 195–208, doi:10.1016/j.devcel.2020.09.001.","ista":"Chaigne A, Labouesse C, White IJ, Agnew M, Hannezo EB, Chalut KJ, Paluch EK. 2020. Abscission couples cell division to embryonic stem cell fate. Developmental Cell. 55(2), 195–208.","chicago":"Chaigne, Agathe, Céline Labouesse, Ian J. White, Meghan Agnew, Edouard B Hannezo, Kevin J. Chalut, and Ewa K. Paluch. “Abscission Couples Cell Division to Embryonic Stem Cell Fate.” Developmental Cell. Elsevier, 2020. https://doi.org/10.1016/j.devcel.2020.09.001."},"volume":55,"issue":"2","language":[{"iso":"eng"}],"file":[{"date_updated":"2021-02-04T10:20:02Z","file_size":6929686,"creator":"dernst","date_created":"2021-02-04T10:20:02Z","file_name":"2020_DevelopmCell_Chaigne.pdf","content_type":"application/pdf","access_level":"open_access","relation":"main_file","checksum":"88e1a031a61689165d19a19c2f16d795","file_id":"9086","success":1}],"publication_status":"published","publication_identifier":{"issn":["15345807"],"eissn":["18781551"]},"intvolume":" 55","month":"10","scopus_import":"1","oa_version":"Published Version","pmid":1,"abstract":[{"lang":"eng","text":"Cell fate transitions are key to development and homeostasis. It is thus essential to understand the cellular mechanisms controlling fate transitions. Cell division has been implicated in fate decisions in many stem cell types, including neuronal and epithelial progenitors. In other stem cells, such as embryonic stem (ES) cells, the role of division remains unclear. Here, we show that exit from naive pluripotency in mouse ES cells generally occurs after a division. We further show that exit timing is strongly correlated between sister cells, which remain connected by cytoplasmic bridges long after division, and that bridge abscission progressively accelerates as cells exit naive pluripotency. Finally, interfering with abscission impairs naive pluripotency exit, and artificially inducing abscission accelerates it. Altogether, our data indicate that a switch in the division machinery leading to faster abscission regulates pluripotency exit. Our study identifies abscission as a key cellular process coupling cell division to fate transitions."}],"file_date_updated":"2021-02-04T10:20:02Z","department":[{"_id":"EdHa"}],"ddc":["570"],"date_updated":"2023-08-22T10:16:58Z","status":"public","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"type":"journal_article","article_type":"original","_id":"8672"},{"issue":"8","volume":21,"publication_identifier":{"issn":["1465-7392","1476-4679"]},"publication_status":"published","language":[{"iso":"eng"}],"main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978139/","open_access":"1"}],"month":"08","intvolume":" 21","abstract":[{"text":"The sebaceous gland (SG) is an essential component of the skin, and SG dysfunction is debilitating1,2. Yet, the cellular bases for its origin, development and subsequent maintenance remain poorly understood. Here, we apply large-scale quantitative fate mapping to define the patterns of cell fate behaviour during SG development and maintenance. We show that the SG develops from a defined number of lineage-restricted progenitors that undergo a programme of independent and stochastic cell fate decisions. Following an expansion phase, equipotent progenitors transition into a phase of homeostatic turnover, which is correlated with changes in the mechanical properties of the stroma and spatial restrictions on gland size. Expression of the oncogene KrasG12D results in a release from these constraints and unbridled gland expansion. Quantitative clonal fate analysis reveals that, during this phase, the primary effect of the Kras oncogene is to drive a constant fate bias with little effect on cell division rates. These findings provide insight into the developmental programme of the SG, as well as the mechanisms that drive tumour progression and gland dysfunction.","lang":"eng"}],"pmid":1,"oa_version":"Submitted Version","date_updated":"2021-01-12T08:13:47Z","extern":"1","article_type":"original","type":"journal_article","status":"public","_id":"7476","page":"924-932","date_published":"2019-08-01T00:00:00Z","doi":"10.1038/s41556-019-0362-x","date_created":"2020-02-11T08:43:49Z","year":"2019","day":"01","publication":"Nature Cell Biology","quality_controlled":"1","publisher":"Springer Nature","oa":1,"author":[{"first_name":"Marianne Stemann","full_name":"Andersen, Marianne Stemann","last_name":"Andersen"},{"first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","last_name":"Hannezo"},{"first_name":"Svetlana","last_name":"Ulyanchenko","full_name":"Ulyanchenko, Svetlana"},{"full_name":"Estrach, Soline","last_name":"Estrach","first_name":"Soline"},{"first_name":"Yasuko","full_name":"Antoku, Yasuko","last_name":"Antoku"},{"first_name":"Sabrina","last_name":"Pisano","full_name":"Pisano, Sabrina"},{"first_name":"Kim E.","last_name":"Boonekamp","full_name":"Boonekamp, Kim E."},{"first_name":"Sarah","full_name":"Sendrup, Sarah","last_name":"Sendrup"},{"last_name":"Maimets","full_name":"Maimets, Martti","first_name":"Martti"},{"full_name":"Pedersen, Marianne Terndrup","last_name":"Pedersen","first_name":"Marianne Terndrup"},{"first_name":"Jens V.","last_name":"Johansen","full_name":"Johansen, Jens V."},{"last_name":"Clement","full_name":"Clement, Ditte L.","first_name":"Ditte L."},{"last_name":"Feral","full_name":"Feral, Chloe C.","first_name":"Chloe C."},{"last_name":"Simons","full_name":"Simons, Benjamin D.","first_name":"Benjamin D."},{"first_name":"Kim B.","full_name":"Jensen, Kim B.","last_name":"Jensen"}],"external_id":{"pmid":["31358966"]},"article_processing_charge":"No","title":"Tracing the cellular dynamics of sebaceous gland development in normal and perturbed states","citation":{"mla":"Andersen, Marianne Stemann, et al. “Tracing the Cellular Dynamics of Sebaceous Gland Development in Normal and Perturbed States.” Nature Cell Biology, vol. 21, no. 8, Springer Nature, 2019, pp. 924–32, doi:10.1038/s41556-019-0362-x.","ieee":"M. S. Andersen et al., “Tracing the cellular dynamics of sebaceous gland development in normal and perturbed states,” Nature Cell Biology, vol. 21, no. 8. Springer Nature, pp. 924–932, 2019.","short":"M.S. Andersen, E.B. Hannezo, S. Ulyanchenko, S. Estrach, Y. Antoku, S. Pisano, K.E. Boonekamp, S. Sendrup, M. Maimets, M.T. Pedersen, J.V. Johansen, D.L. Clement, C.C. Feral, B.D. Simons, K.B. Jensen, Nature Cell Biology 21 (2019) 924–932.","ama":"Andersen MS, Hannezo EB, Ulyanchenko S, et al. Tracing the cellular dynamics of sebaceous gland development in normal and perturbed states. Nature Cell Biology. 2019;21(8):924-932. doi:10.1038/s41556-019-0362-x","apa":"Andersen, M. S., Hannezo, E. B., Ulyanchenko, S., Estrach, S., Antoku, Y., Pisano, S., … Jensen, K. B. (2019). Tracing the cellular dynamics of sebaceous gland development in normal and perturbed states. Nature Cell Biology. Springer Nature. https://doi.org/10.1038/s41556-019-0362-x","chicago":"Andersen, Marianne Stemann, Edouard B Hannezo, Svetlana Ulyanchenko, Soline Estrach, Yasuko Antoku, Sabrina Pisano, Kim E. Boonekamp, et al. “Tracing the Cellular Dynamics of Sebaceous Gland Development in Normal and Perturbed States.” Nature Cell Biology. Springer Nature, 2019. https://doi.org/10.1038/s41556-019-0362-x.","ista":"Andersen MS, Hannezo EB, Ulyanchenko S, Estrach S, Antoku Y, Pisano S, Boonekamp KE, Sendrup S, Maimets M, Pedersen MT, Johansen JV, Clement DL, Feral CC, Simons BD, Jensen KB. 2019. Tracing the cellular dynamics of sebaceous gland development in normal and perturbed states. Nature Cell Biology. 21(8), 924–932."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"},{"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"chicago":"Recho, Pierre, Adrien Hallou, and Edouard B Hannezo. “Theory of Mechanochemical Patterning in Biphasic Biological Tissues.” Proceedings of the National Academy of Sciences of the United States of America. National Academy of Sciences, 2019. https://doi.org/10.1073/pnas.1813255116.","ista":"Recho P, Hallou A, Hannezo EB. 2019. Theory of mechanochemical patterning in biphasic biological tissues. Proceedings of the National Academy of Sciences of the United States of America. 116(12), 5344–5349.","mla":"Recho, Pierre, et al. “Theory of Mechanochemical Patterning in Biphasic Biological Tissues.” Proceedings of the National Academy of Sciences of the United States of America, vol. 116, no. 12, National Academy of Sciences, 2019, pp. 5344–49, doi:10.1073/pnas.1813255116.","short":"P. Recho, A. Hallou, E.B. Hannezo, Proceedings of the National Academy of Sciences of the United States of America 116 (2019) 5344–5349.","ieee":"P. Recho, A. Hallou, and E. B. Hannezo, “Theory of mechanochemical patterning in biphasic biological tissues,” Proceedings of the National Academy of Sciences of the United States of America, vol. 116, no. 12. National Academy of Sciences, pp. 5344–5349, 2019.","apa":"Recho, P., Hallou, A., & Hannezo, E. B. (2019). Theory of mechanochemical patterning in biphasic biological tissues. Proceedings of the National Academy of Sciences of the United States of America. National Academy of Sciences. https://doi.org/10.1073/pnas.1813255116","ama":"Recho P, Hallou A, Hannezo EB. Theory of mechanochemical patterning in biphasic biological tissues. Proceedings of the National Academy of Sciences of the United States of America. 2019;116(12):5344-5349. doi:10.1073/pnas.1813255116"},"title":"Theory of mechanochemical patterning in biphasic biological tissues","author":[{"first_name":"Pierre","last_name":"Recho","full_name":"Recho, Pierre"},{"first_name":"Adrien","full_name":"Hallou, Adrien","last_name":"Hallou"},{"last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"}],"article_processing_charge":"No","external_id":{"pmid":["30819884"],"isi":["000461679000027"]},"project":[{"name":"Active mechano-chemical description of the cell cytoskeleton","grant_number":"P31639","call_identifier":"FWF","_id":"268294B6-B435-11E9-9278-68D0E5697425"}],"day":"19","publication":"Proceedings of the National Academy of Sciences of the United States of America","has_accepted_license":"1","isi":1,"year":"2019","doi":"10.1073/pnas.1813255116","date_published":"2019-03-19T00:00:00Z","date_created":"2019-03-31T21:59:13Z","page":"5344-5349","quality_controlled":"1","publisher":"National Academy of Sciences","oa":1,"ddc":["570"],"date_updated":"2023-08-25T08:57:30Z","department":[{"_id":"EdHa"}],"file_date_updated":"2020-07-14T12:47:23Z","_id":"6191","status":"public","type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"file":[{"date_created":"2019-04-03T14:10:30Z","file_name":"2019_PNAS_Recho.pdf","creator":"dernst","date_updated":"2020-07-14T12:47:23Z","file_size":3456045,"file_id":"6193","checksum":"8b67eee0ea8e5db61583e4d485215258","access_level":"open_access","relation":"main_file","content_type":"application/pdf"}],"language":[{"iso":"eng"}],"publication_identifier":{"issn":["00278424"],"eissn":["10916490"]},"publication_status":"published","volume":116,"issue":"12","related_material":{"link":[{"relation":"supplementary_material","url":"www.pnas.org/lookup/suppl/doi:10.1073/pnas.1813255116/-/DCSupplemental"}]},"pmid":1,"oa_version":"Published Version","abstract":[{"lang":"eng","text":"The formation of self-organized patterns is key to the morphogenesis of multicellular organisms, although a comprehensive theory of biological pattern formation is still lacking. Here, we propose a minimal model combining tissue mechanics with morphogen turnover and transport to explore routes to patterning. Our active description couples morphogen reaction and diffusion, which impact cell differentiation and tissue mechanics, to a two-phase poroelastic rheology, where one tissue phase consists of a poroelastic cell network and the other one of a permeating extracellular fluid, which provides a feedback by actively transporting morphogens. While this model encompasses previous theories approximating tissues to inert monophasic media, such as Turing’s reaction–diffusion model, it overcomes some of their key limitations permitting pattern formation via any two-species biochemical kinetics due to mechanically induced cross-diffusion flows. Moreover, we describe a qualitatively different advection-driven Keller–Segel instability which allows for the formation of patterns with a single morphogen and whose fundamental mode pattern robustly scales with tissue size. We discuss the potential relevance of these findings for tissue morphogenesis."}],"month":"03","intvolume":" 116","scopus_import":"1"},{"pmid":1,"oa_version":"Submitted Version","abstract":[{"lang":"eng","text":"Adult intestinal stem cells are located at the bottom of crypts of Lieberkühn, where they express markers such as LGR5 1,2 and fuel the constant replenishment of the intestinal epithelium1. Although fetal LGR5-expressing cells can give rise to adult intestinal stem cells3,4, it remains unclear whether this population in the patterned epithelium represents unique intestinal stem-cell precursors. Here we show, using unbiased quantitative lineage-tracing approaches, biophysical modelling and intestinal transplantation, that all cells of the mouse intestinal epithelium—irrespective of their location and pattern of LGR5 expression in the fetal gut tube—contribute actively to the adult intestinal stem cell pool. Using 3D imaging, we find that during fetal development the villus undergoes gross remodelling and fission. This brings epithelial cells from the non-proliferative villus into the proliferative intervillus region, which enables them to contribute to the adult stem-cell niche. Our results demonstrate that large-scale remodelling of the intestinal wall and cell-fate specification are closely linked. Moreover, these findings provide a direct link between the observed plasticity and cellular reprogramming of differentiating cells in adult tissues following damage5,6,7,8,9, revealing that stem-cell identity is an induced rather than a hardwired property."}],"intvolume":" 570","month":"06","main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6986928"}],"scopus_import":"1","language":[{"iso":"eng"}],"publication_status":"published","publication_identifier":{"issn":["00280836"],"eissn":["14764687"]},"volume":570,"_id":"6513","status":"public","type":"journal_article","article_type":"original","date_updated":"2023-08-28T09:30:23Z","department":[{"_id":"EdHa"}],"oa":1,"quality_controlled":"1","publisher":"Springer Nature","publication":"Nature","day":"06","year":"2019","isi":1,"date_created":"2019-06-02T21:59:14Z","date_published":"2019-06-06T00:00:00Z","doi":"10.1038/s41586-019-1212-5","page":"107-111","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"mla":"Guiu, Jordi, et al. “Tracing the Origin of Adult Intestinal Stem Cells.” Nature, vol. 570, Springer Nature, 2019, pp. 107–11, doi:10.1038/s41586-019-1212-5.","ieee":"J. Guiu et al., “Tracing the origin of adult intestinal stem cells,” Nature, vol. 570. Springer Nature, pp. 107–111, 2019.","short":"J. Guiu, E.B. Hannezo, S. Yui, S. Demharter, S. Ulyanchenko, M. Maimets, A. Jørgensen, S. Perlman, L. Lundvall, L.S. Mamsen, A. Larsen, R.H. Olesen, C.Y. Andersen, L.L. Thuesen, K.J. Hare, T.H. Pers, K. Khodosevich, B.D. Simons, K.B. Jensen, Nature 570 (2019) 107–111.","apa":"Guiu, J., Hannezo, E. B., Yui, S., Demharter, S., Ulyanchenko, S., Maimets, M., … Jensen, K. B. (2019). Tracing the origin of adult intestinal stem cells. Nature. Springer Nature. https://doi.org/10.1038/s41586-019-1212-5","ama":"Guiu J, Hannezo EB, Yui S, et al. Tracing the origin of adult intestinal stem cells. Nature. 2019;570:107-111. doi:10.1038/s41586-019-1212-5","chicago":"Guiu, Jordi, Edouard B Hannezo, Shiro Yui, Samuel Demharter, Svetlana Ulyanchenko, Martti Maimets, Anne Jørgensen, et al. “Tracing the Origin of Adult Intestinal Stem Cells.” Nature. Springer Nature, 2019. https://doi.org/10.1038/s41586-019-1212-5.","ista":"Guiu J, Hannezo EB, Yui S, Demharter S, Ulyanchenko S, Maimets M, Jørgensen A, Perlman S, Lundvall L, Mamsen LS, Larsen A, Olesen RH, Andersen CY, Thuesen LL, Hare KJ, Pers TH, Khodosevich K, Simons BD, Jensen KB. 2019. Tracing the origin of adult intestinal stem cells. Nature. 570, 107–111."},"title":"Tracing the origin of adult intestinal stem cells","article_processing_charge":"No","external_id":{"isi":["000470149000048"],"pmid":["31092921"]},"author":[{"first_name":"Jordi","full_name":"Guiu, Jordi","last_name":"Guiu"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561"},{"last_name":"Yui","full_name":"Yui, Shiro","first_name":"Shiro"},{"full_name":"Demharter, Samuel","last_name":"Demharter","first_name":"Samuel"},{"first_name":"Svetlana","full_name":"Ulyanchenko, Svetlana","last_name":"Ulyanchenko"},{"first_name":"Martti","last_name":"Maimets","full_name":"Maimets, Martti"},{"last_name":"Jørgensen","full_name":"Jørgensen, Anne","first_name":"Anne"},{"first_name":"Signe","full_name":"Perlman, Signe","last_name":"Perlman"},{"first_name":"Lene","full_name":"Lundvall, Lene","last_name":"Lundvall"},{"full_name":"Mamsen, Linn Salto","last_name":"Mamsen","first_name":"Linn Salto"},{"first_name":"Agnete","last_name":"Larsen","full_name":"Larsen, Agnete"},{"first_name":"Rasmus H.","last_name":"Olesen","full_name":"Olesen, Rasmus H."},{"first_name":"Claus Yding","full_name":"Andersen, Claus Yding","last_name":"Andersen"},{"first_name":"Lea Langhoff","full_name":"Thuesen, Lea Langhoff","last_name":"Thuesen"},{"full_name":"Hare, Kristine Juul","last_name":"Hare","first_name":"Kristine Juul"},{"first_name":"Tune H.","full_name":"Pers, Tune H.","last_name":"Pers"},{"full_name":"Khodosevich, Konstantin","last_name":"Khodosevich","first_name":"Konstantin"},{"full_name":"Simons, Benjamin D.","last_name":"Simons","first_name":"Benjamin D."},{"first_name":"Kim B.","full_name":"Jensen, Kim B.","last_name":"Jensen"}]},{"volume":60,"language":[{"iso":"eng"}],"publication_status":"published","publication_identifier":{"issn":["09550674"],"eissn":["18790410"]},"intvolume":" 60","month":"10","scopus_import":"1","oa_version":"None","pmid":1,"abstract":[{"text":"Branching morphogenesis is a prototypical example of complex three-dimensional organ sculpting, required in multiple developmental settings to maximize the area of exchange surfaces. It requires, in particular, the coordinated growth of different cell types together with complex patterning to lead to robust macroscopic outputs. In recent years, novel multiscale quantitative biology approaches, together with biophysical modelling, have begun to shed new light of this topic. Here, we wish to review some of these recent developments, highlighting the generic design principles that can be abstracted across different branched organs, as well as the implications for the broader fields of stem cell, developmental and systems biology.","lang":"eng"}],"department":[{"_id":"EdHa"}],"date_updated":"2023-08-28T09:38:57Z","status":"public","type":"journal_article","article_type":"original","_id":"6559","date_created":"2019-06-16T21:59:12Z","doi":"10.1016/j.ceb.2019.04.008","date_published":"2019-10-01T00:00:00Z","page":"99-105","publication":"Current Opinion in Cell Biology","day":"01","year":"2019","isi":1,"publisher":"Elsevier","quality_controlled":"1","title":"Multiscale dynamics of branching morphogenesis","article_processing_charge":"No","external_id":{"pmid":["31181348"],"isi":["000486545800014"]},"author":[{"first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","last_name":"Hannezo"},{"first_name":"Benjamin D.","last_name":"Simons","full_name":"Simons, Benjamin D."}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"ista":"Hannezo EB, Simons BD. 2019. Multiscale dynamics of branching morphogenesis. Current Opinion in Cell Biology. 60, 99–105.","chicago":"Hannezo, Edouard B, and Benjamin D. Simons. “Multiscale Dynamics of Branching Morphogenesis.” Current Opinion in Cell Biology. Elsevier, 2019. https://doi.org/10.1016/j.ceb.2019.04.008.","apa":"Hannezo, E. B., & Simons, B. D. (2019). Multiscale dynamics of branching morphogenesis. Current Opinion in Cell Biology. Elsevier. https://doi.org/10.1016/j.ceb.2019.04.008","ama":"Hannezo EB, Simons BD. Multiscale dynamics of branching morphogenesis. Current Opinion in Cell Biology. 2019;60:99-105. doi:10.1016/j.ceb.2019.04.008","short":"E.B. Hannezo, B.D. Simons, Current Opinion in Cell Biology 60 (2019) 99–105.","ieee":"E. B. Hannezo and B. D. Simons, “Multiscale dynamics of branching morphogenesis,” Current Opinion in Cell Biology, vol. 60. Elsevier, pp. 99–105, 2019.","mla":"Hannezo, Edouard B., and Benjamin D. Simons. “Multiscale Dynamics of Branching Morphogenesis.” Current Opinion in Cell Biology, vol. 60, Elsevier, 2019, pp. 99–105, doi:10.1016/j.ceb.2019.04.008."}},{"_id":"6601","type":"journal_article","article_type":"review","status":"public","date_updated":"2023-08-28T12:25:21Z","department":[{"_id":"CaHe"},{"_id":"EdHa"}],"abstract":[{"text":"There is increasing evidence that both mechanical and biochemical signals play important roles in development and disease. The development of complex organisms, in particular, has been proposed to rely on the feedback between mechanical and biochemical patterning events. This feedback occurs at the molecular level via mechanosensation but can also arise as an emergent property of the system at the cellular and tissue level. In recent years, dynamic changes in tissue geometry, flow, rheology, and cell fate specification have emerged as key platforms of mechanochemical feedback loops in multiple processes. Here, we review recent experimental and theoretical advances in understanding how these feedbacks function in development and disease.","lang":"eng"}],"pmid":1,"oa_version":"Published Version","main_file_link":[{"url":"https://doi.org/10.1016/j.cell.2019.05.052","open_access":"1"}],"scopus_import":"1","intvolume":" 178","month":"07","publication_status":"published","publication_identifier":{"issn":["00928674"]},"language":[{"iso":"eng"}],"ec_funded":1,"issue":"1","volume":178,"project":[{"_id":"260F1432-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","grant_number":"742573"},{"name":"Active mechano-chemical description of the cell cytoskeleton","grant_number":"P31639","call_identifier":"FWF","_id":"268294B6-B435-11E9-9278-68D0E5697425"}],"citation":{"mla":"Hannezo, Edouard B., and Carl-Philipp J. Heisenberg. “Mechanochemical Feedback Loops in Development and Disease.” Cell, vol. 178, no. 1, Elsevier, 2019, pp. 12–25, doi:10.1016/j.cell.2019.05.052.","ieee":"E. B. Hannezo and C.-P. J. Heisenberg, “Mechanochemical feedback loops in development and disease,” Cell, vol. 178, no. 1. Elsevier, pp. 12–25, 2019.","short":"E.B. Hannezo, C.-P.J. Heisenberg, Cell 178 (2019) 12–25.","apa":"Hannezo, E. B., & Heisenberg, C.-P. J. (2019). Mechanochemical feedback loops in development and disease. Cell. Elsevier. https://doi.org/10.1016/j.cell.2019.05.052","ama":"Hannezo EB, Heisenberg C-PJ. Mechanochemical feedback loops in development and disease. Cell. 2019;178(1):12-25. doi:10.1016/j.cell.2019.05.052","chicago":"Hannezo, Edouard B, and Carl-Philipp J Heisenberg. “Mechanochemical Feedback Loops in Development and Disease.” Cell. Elsevier, 2019. https://doi.org/10.1016/j.cell.2019.05.052.","ista":"Hannezo EB, Heisenberg C-PJ. 2019. Mechanochemical feedback loops in development and disease. Cell. 178(1), 12–25."},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","external_id":{"isi":["000473002700005"],"pmid":["31251912"]},"article_processing_charge":"No","author":[{"last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg"}],"title":"Mechanochemical feedback loops in development and disease","oa":1,"publisher":"Elsevier","quality_controlled":"1","year":"2019","isi":1,"publication":"Cell","day":"27","page":"12-25","date_created":"2019-06-30T21:59:11Z","date_published":"2019-07-27T00:00:00Z","doi":"10.1016/j.cell.2019.05.052"},{"publisher":"American Association for the Advancement of Science","quality_controlled":"1","publication":"Science","day":"16","year":"2019","isi":1,"date_created":"2019-08-25T22:00:51Z","doi":"10.1126/science.aau3429","date_published":"2019-08-16T00:00:00Z","page":"705-710","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"ama":"Krndija D, Marjou FE, Guirao B, et al. Active cell migration is critical for steady-state epithelial turnover in the gut. Science. 2019;365(6454):705-710. doi:10.1126/science.aau3429","apa":"Krndija, D., Marjou, F. E., Guirao, B., Richon, S., Leroy, O., Bellaiche, Y., … Vignjevic, D. M. (2019). Active cell migration is critical for steady-state epithelial turnover in the gut. Science. American Association for the Advancement of Science. https://doi.org/10.1126/science.aau3429","ieee":"D. Krndija et al., “Active cell migration is critical for steady-state epithelial turnover in the gut,” Science, vol. 365, no. 6454. American Association for the Advancement of Science, pp. 705–710, 2019.","short":"D. Krndija, F.E. Marjou, B. Guirao, S. Richon, O. Leroy, Y. Bellaiche, E.B. Hannezo, D.M. Vignjevic, Science 365 (2019) 705–710.","mla":"Krndija, Denis, et al. “Active Cell Migration Is Critical for Steady-State Epithelial Turnover in the Gut.” Science, vol. 365, no. 6454, American Association for the Advancement of Science, 2019, pp. 705–10, doi:10.1126/science.aau3429.","ista":"Krndija D, Marjou FE, Guirao B, Richon S, Leroy O, Bellaiche Y, Hannezo EB, Vignjevic DM. 2019. Active cell migration is critical for steady-state epithelial turnover in the gut. Science. 365(6454), 705–710.","chicago":"Krndija, Denis, Fatima El Marjou, Boris Guirao, Sophie Richon, Olivier Leroy, Yohanns Bellaiche, Edouard B Hannezo, and Danijela Matic Vignjevic. “Active Cell Migration Is Critical for Steady-State Epithelial Turnover in the Gut.” Science. American Association for the Advancement of Science, 2019. https://doi.org/10.1126/science.aau3429."},"title":"Active cell migration is critical for steady-state epithelial turnover in the gut","external_id":{"isi":["000481688700050"],"pmid":["31416964"]},"article_processing_charge":"No","author":[{"full_name":"Krndija, Denis","last_name":"Krndija","first_name":"Denis"},{"first_name":"Fatima El","full_name":"Marjou, Fatima El","last_name":"Marjou"},{"full_name":"Guirao, Boris","last_name":"Guirao","first_name":"Boris"},{"first_name":"Sophie","last_name":"Richon","full_name":"Richon, Sophie"},{"last_name":"Leroy","full_name":"Leroy, Olivier","first_name":"Olivier"},{"first_name":"Yohanns","full_name":"Bellaiche, Yohanns","last_name":"Bellaiche"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","last_name":"Hannezo","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B"},{"last_name":"Vignjevic","full_name":"Vignjevic, Danijela Matic","first_name":"Danijela Matic"}],"pmid":1,"oa_version":"None","abstract":[{"lang":"eng","text":"Steady-state turnover is a hallmark of epithelial tissues throughout adult life. Intestinal epithelial turnover is marked by continuous cell migration, which is assumed to be driven by mitotic pressure from the crypts. However, the balance of forces in renewal remains ill-defined. Combining biophysical modeling and quantitative three-dimensional tissue imaging with genetic and physical manipulations, we revealed the existence of an actin-related protein 2/3 complex–dependent active migratory force, which explains quantitatively the profiles of cell speed, density, and tissue tension along the villi. Cells migrate collectively with minimal rearrangements while displaying dual—apicobasal and front-back—polarity characterized by actin-rich basal protrusions oriented in the direction of migration. We propose that active migration is a critical component of gut epithelial turnover."}],"intvolume":" 365","month":"08","scopus_import":"1","language":[{"iso":"eng"}],"publication_status":"published","volume":365,"issue":"6454","_id":"6832","status":"public","type":"journal_article","date_updated":"2023-08-29T07:16:40Z","department":[{"_id":"EdHa"}]},{"quality_controlled":"1","publisher":"Nature Publishing Group","oa":1,"day":"01","publication":"Nature Cell Biology","has_accepted_license":"1","isi":1,"year":"2019","date_published":"2019-02-01T00:00:00Z","doi":"10.1038/s41556-018-0247-4","date_created":"2018-12-30T22:59:15Z","page":"169–178","project":[{"grant_number":"742573","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","_id":"260F1432-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"},{"name":"Molecular mechanism of auxindriven formative divisions delineating lateral root organogenesis in plants (EMBO fellowship)","grant_number":"ALTF710-2016","_id":"253E54C8-B435-11E9-9278-68D0E5697425"}],"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","citation":{"mla":"Petridou, Nicoletta, et al. “Fluidization-Mediated Tissue Spreading by Mitotic Cell Rounding and Non-Canonical Wnt Signalling.” Nature Cell Biology, vol. 21, Nature Publishing Group, 2019, pp. 169–178, doi:10.1038/s41556-018-0247-4.","ieee":"N. Petridou, S. Grigolon, G. Salbreux, E. B. Hannezo, and C.-P. J. Heisenberg, “Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling,” Nature Cell Biology, vol. 21. Nature Publishing Group, pp. 169–178, 2019.","short":"N. Petridou, S. Grigolon, G. Salbreux, E.B. Hannezo, C.-P.J. Heisenberg, Nature Cell Biology 21 (2019) 169–178.","ama":"Petridou N, Grigolon S, Salbreux G, Hannezo EB, Heisenberg C-PJ. Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling. Nature Cell Biology. 2019;21:169–178. doi:10.1038/s41556-018-0247-4","apa":"Petridou, N., Grigolon, S., Salbreux, G., Hannezo, E. B., & Heisenberg, C.-P. J. (2019). Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling. Nature Cell Biology. Nature Publishing Group. https://doi.org/10.1038/s41556-018-0247-4","chicago":"Petridou, Nicoletta, Silvia Grigolon, Guillaume Salbreux, Edouard B Hannezo, and Carl-Philipp J Heisenberg. “Fluidization-Mediated Tissue Spreading by Mitotic Cell Rounding and Non-Canonical Wnt Signalling.” Nature Cell Biology. Nature Publishing Group, 2019. https://doi.org/10.1038/s41556-018-0247-4.","ista":"Petridou N, Grigolon S, Salbreux G, Hannezo EB, Heisenberg C-PJ. 2019. Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling. Nature Cell Biology. 21, 169–178."},"title":"Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling","author":[{"full_name":"Petridou, Nicoletta","orcid":"0000-0002-8451-1195","last_name":"Petridou","first_name":"Nicoletta","id":"2A003F6C-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Silvia","full_name":"Grigolon, Silvia","last_name":"Grigolon"},{"first_name":"Guillaume","full_name":"Salbreux, Guillaume","last_name":"Salbreux"},{"last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"},{"first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"article_processing_charge":"No","external_id":{"isi":["000457468300011"],"pmid":["30559456"]},"oa_version":"Submitted Version","pmid":1,"abstract":[{"lang":"eng","text":"Tissue morphogenesis is driven by mechanical forces that elicit changes in cell size, shape and motion. The extent by which forces deform tissues critically depends on the rheological properties of the recipient tissue. Yet, whether and how dynamic changes in tissue rheology affect tissue morphogenesis and how they are regulated within the developing organism remain unclear. Here, we show that blastoderm spreading at the onset of zebrafish morphogenesis relies on a rapid, pronounced and spatially patterned tissue fluidization. Blastoderm fluidization is temporally controlled by mitotic cell rounding-dependent cell–cell contact disassembly during the last rounds of cell cleavages. Moreover, fluidization is spatially restricted to the central blastoderm by local activation of non-canonical Wnt signalling within the blastoderm margin, increasing cell cohesion and thereby counteracting the effect of mitotic rounding on contact disassembly. Overall, our results identify a fluidity transition mediated by loss of cell cohesion as a critical regulator of embryo morphogenesis."}],"acknowledged_ssus":[{"_id":"Bio"}],"month":"02","intvolume":" 21","scopus_import":"1","file":[{"success":1,"checksum":"e38523787b3bc84006f2793de99ad70f","file_id":"8685","relation":"main_file","access_level":"open_access","content_type":"application/pdf","file_name":"2018_NatureCellBio_Petridou_accepted.pdf","date_created":"2020-10-21T07:18:35Z","creator":"dernst","file_size":71590590,"date_updated":"2020-10-21T07:18:35Z"}],"language":[{"iso":"eng"}],"publication_identifier":{"issn":["14657392"]},"publication_status":"published","related_material":{"link":[{"relation":"press_release","url":"https://ist.ac.at/en/news/when-a-fish-becomes-fluid/","description":"News on IST Homepage"}]},"volume":21,"ec_funded":1,"_id":"5789","status":"public","article_type":"original","type":"journal_article","ddc":["570"],"date_updated":"2023-09-11T14:03:28Z","department":[{"_id":"CaHe"},{"_id":"EdHa"}],"file_date_updated":"2020-10-21T07:18:35Z"},{"ddc":["570"],"date_updated":"2024-03-27T23:30:38Z","file_date_updated":"2020-10-21T07:22:34Z","department":[{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"BjHo"}],"_id":"6508","status":"public","type":"journal_article","article_type":"original","language":[{"iso":"eng"}],"file":[{"content_type":"application/pdf","access_level":"open_access","relation":"main_file","checksum":"aea43726d80e35ce3885073a5f05c3e3","file_id":"8686","success":1,"date_updated":"2020-10-21T07:22:34Z","file_size":3356292,"creator":"dernst","date_created":"2020-10-21T07:22:34Z","file_name":"2019_Cell_Shamipour_accepted.pdf"}],"publication_status":"published","publication_identifier":{"issn":["00928674"],"eissn":["10974172"]},"ec_funded":1,"volume":177,"issue":"6","related_material":{"record":[{"relation":"dissertation_contains","id":"8350","status":"public"}],"link":[{"description":"News on IST Homepage","url":"https://ist.ac.at/en/news/how-the-cytoplasm-separates-from-the-yolk/","relation":"press_release"}]},"oa_version":"Published Version","pmid":1,"acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"}],"abstract":[{"text":"Segregation of maternal determinants within the oocyte constitutes the first step in embryo patterning. In zebrafish oocytes, extensive ooplasmic streaming leads to the segregation of ooplasm from yolk granules along the animal-vegetal axis of the oocyte. Here, we show that this process does not rely on cortical actin reorganization, as previously thought, but instead on a cell-cycle-dependent bulk actin polymerization wave traveling from the animal to the vegetal pole of the oocyte. This wave functions in segregation by both pulling ooplasm animally and pushing yolk granules vegetally. Using biophysical experimentation and theory, we show that ooplasm pulling is mediated by bulk actin network flows exerting friction forces on the ooplasm, while yolk granule pushing is achieved by a mechanism closely resembling actin comet formation on yolk granules. Our study defines a novel role of cell-cycle-controlled bulk actin polymerization waves in oocyte polarization via ooplasmic segregation.","lang":"eng"}],"intvolume":" 177","month":"05","main_file_link":[{"url":"https://doi.org/10.1016/j.cell.2019.04.030","open_access":"1"}],"scopus_import":"1","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"ista":"Shamipour S, Kardos R, Xue S, Hof B, Hannezo EB, Heisenberg C-PJ. 2019. Bulk actin dynamics drive phase segregation in zebrafish oocytes. Cell. 177(6), 1463–1479.e18.","chicago":"Shamipour, Shayan, Roland Kardos, Shi-lei Xue, Björn Hof, Edouard B Hannezo, and Carl-Philipp J Heisenberg. “Bulk Actin Dynamics Drive Phase Segregation in Zebrafish Oocytes.” Cell. Elsevier, 2019. https://doi.org/10.1016/j.cell.2019.04.030.","ieee":"S. Shamipour, R. Kardos, S. Xue, B. Hof, E. B. Hannezo, and C.-P. J. Heisenberg, “Bulk actin dynamics drive phase segregation in zebrafish oocytes,” Cell, vol. 177, no. 6. Elsevier, p. 1463–1479.e18, 2019.","short":"S. Shamipour, R. Kardos, S. Xue, B. Hof, E.B. Hannezo, C.-P.J. Heisenberg, Cell 177 (2019) 1463–1479.e18.","ama":"Shamipour S, Kardos R, Xue S, Hof B, Hannezo EB, Heisenberg C-PJ. Bulk actin dynamics drive phase segregation in zebrafish oocytes. Cell. 2019;177(6):1463-1479.e18. doi:10.1016/j.cell.2019.04.030","apa":"Shamipour, S., Kardos, R., Xue, S., Hof, B., Hannezo, E. B., & Heisenberg, C.-P. J. (2019). Bulk actin dynamics drive phase segregation in zebrafish oocytes. Cell. Elsevier. https://doi.org/10.1016/j.cell.2019.04.030","mla":"Shamipour, Shayan, et al. “Bulk Actin Dynamics Drive Phase Segregation in Zebrafish Oocytes.” Cell, vol. 177, no. 6, Elsevier, 2019, p. 1463–1479.e18, doi:10.1016/j.cell.2019.04.030."},"title":"Bulk actin dynamics drive phase segregation in zebrafish oocytes","article_processing_charge":"No","external_id":{"isi":["000469415100013"],"pmid":["31080065"]},"author":[{"full_name":"Shamipour, Shayan","last_name":"Shamipour","id":"40B34FE2-F248-11E8-B48F-1D18A9856A87","first_name":"Shayan"},{"id":"4039350E-F248-11E8-B48F-1D18A9856A87","first_name":"Roland","full_name":"Kardos, Roland","last_name":"Kardos"},{"first_name":"Shi-lei","id":"31D2C804-F248-11E8-B48F-1D18A9856A87","full_name":"Xue, Shi-lei","last_name":"Xue"},{"last_name":"Hof","full_name":"Hof, Björn","orcid":"0000-0003-2057-2754","id":"3A374330-F248-11E8-B48F-1D18A9856A87","first_name":"Björn"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","last_name":"Hannezo"},{"last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"project":[{"grant_number":"742573","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","_id":"260F1432-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"},{"name":"Active mechano-chemical description of the cell cytoskeleton","grant_number":"P31639","_id":"268294B6-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"}],"publication":"Cell","day":"30","year":"2019","isi":1,"has_accepted_license":"1","date_created":"2019-06-02T21:59:12Z","date_published":"2019-05-30T00:00:00Z","doi":"10.1016/j.cell.2019.04.030","page":"1463-1479.e18","acknowledgement":"We would like to thank Pierre Recho, Guillaume Salbreux, and Silvia Grigolon for advice on the theory, Lila Solnica-Krezel for kindly providing us with zebrafish dachsous mutants, members of the Heisenberg and Hannezo groups for fruitful discussions, and the Bioimaging and zebrafish facilities at IST Austria for their continuous support. This project has received funding from the European Union (European Research Council Advanced Grant 742573 to C.P.H.) and from the Austrian Science Fund (FWF) (P 31639 to E.H.).","oa":1,"publisher":"Elsevier","quality_controlled":"1"},{"department":[{"_id":"EdHa"}],"file_date_updated":"2020-07-14T12:46:22Z","date_updated":"2023-09-08T11:41:45Z","ddc":["539","570"],"type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"status":"public","pubrep_id":"996","_id":"401","issue":"1","volume":9,"publication_status":"published","file":[{"access_level":"open_access","relation":"main_file","content_type":"application/pdf","checksum":"87a427bc2e8724be3dd22a4efdd21a33","file_id":"4902","creator":"system","date_updated":"2020-07-14T12:46:22Z","file_size":3780491,"date_created":"2018-12-12T10:11:45Z","file_name":"IST-2018-996-v1+1_2018_Hannezo_A-biochemical.pdf"}],"language":[{"iso":"eng"}],"scopus_import":"1","month":"03","intvolume":" 9","abstract":[{"lang":"eng","text":"The actomyosin cytoskeleton, a key stress-producing unit in epithelial cells, oscillates spontaneously in a wide variety of systems. Although much of the signal cascade regulating myosin activity has been characterized, the origin of such oscillatory behavior is still unclear. Here, we show that basal myosin II oscillation in Drosophila ovarian epithelium is not controlled by actomyosin cortical tension, but instead relies on a biochemical oscillator involving ROCK and myosin phosphatase. Key to this oscillation is a diffusive ROCK flow, linking junctional Rho1 to medial actomyosin cortex, and dynamically maintained by a self-activation loop reliant on ROCK kinase activity. In response to the resulting myosin II recruitment, myosin phosphatase is locally enriched and shuts off ROCK and myosin II signals. Coupling Drosophila genetics, live imaging, modeling, and optogenetics, we uncover an intrinsic biochemical oscillator at the core of myosin II regulatory network, shedding light on the spatio-temporal dynamics of force generation."}],"oa_version":"Published Version","author":[{"last_name":"Qin","full_name":"Qin, Xiang","first_name":"Xiang"},{"full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"},{"full_name":"Mangeat, Thomas","last_name":"Mangeat","first_name":"Thomas"},{"first_name":"Chang","full_name":"Liu, Chang","last_name":"Liu"},{"first_name":"Pralay","full_name":"Majumder, Pralay","last_name":"Majumder"},{"full_name":"Liu, Jjiaying","last_name":"Liu","first_name":"Jjiaying"},{"first_name":"Valerie","full_name":"Choesmel Cadamuro, Valerie","last_name":"Choesmel Cadamuro"},{"first_name":"Jocelyn","last_name":"Mcdonald","full_name":"Mcdonald, Jocelyn"},{"first_name":"Yinyao","full_name":"Liu, Yinyao","last_name":"Liu"},{"full_name":"Yi, Bin","last_name":"Yi","first_name":"Bin"},{"full_name":"Wang, Xiaobo","last_name":"Wang","first_name":"Xiaobo"}],"publist_id":"7427","article_processing_charge":"No","external_id":{"isi":["000428165400009"]},"title":"A biochemical network controlling basal myosin oscillation","citation":{"chicago":"Qin, Xiang, Edouard B Hannezo, Thomas Mangeat, Chang Liu, Pralay Majumder, Jjiaying Liu, Valerie Choesmel Cadamuro, et al. “A Biochemical Network Controlling Basal Myosin Oscillation.” Nature Communications. Nature Publishing Group, 2018. https://doi.org/10.1038/s41467-018-03574-5.","ista":"Qin X, Hannezo EB, Mangeat T, Liu C, Majumder P, Liu J, Choesmel Cadamuro V, Mcdonald J, Liu Y, Yi B, Wang X. 2018. A biochemical network controlling basal myosin oscillation. Nature Communications. 9(1), 1210.","mla":"Qin, Xiang, et al. “A Biochemical Network Controlling Basal Myosin Oscillation.” Nature Communications, vol. 9, no. 1, 1210, Nature Publishing Group, 2018, doi:10.1038/s41467-018-03574-5.","ama":"Qin X, Hannezo EB, Mangeat T, et al. A biochemical network controlling basal myosin oscillation. Nature Communications. 2018;9(1). doi:10.1038/s41467-018-03574-5","apa":"Qin, X., Hannezo, E. B., Mangeat, T., Liu, C., Majumder, P., Liu, J., … Wang, X. (2018). A biochemical network controlling basal myosin oscillation. Nature Communications. Nature Publishing Group. https://doi.org/10.1038/s41467-018-03574-5","short":"X. Qin, E.B. Hannezo, T. Mangeat, C. Liu, P. Majumder, J. Liu, V. Choesmel Cadamuro, J. Mcdonald, Y. Liu, B. Yi, X. Wang, Nature Communications 9 (2018).","ieee":"X. Qin et al., “A biochemical network controlling basal myosin oscillation,” Nature Communications, vol. 9, no. 1. Nature Publishing Group, 2018."},"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","article_number":"1210","doi":"10.1038/s41467-018-03574-5","date_published":"2018-03-23T00:00:00Z","date_created":"2018-12-11T11:46:16Z","has_accepted_license":"1","isi":1,"year":"2018","day":"23","publication":"Nature Communications","quality_controlled":"1","publisher":"Nature Publishing Group","oa":1},{"article_processing_charge":"No","external_id":{"pmid":["29784917"],"isi":["000433237300003"]},"publist_id":"7594","author":[{"last_name":"Lilja","full_name":"Lilja, Anna","first_name":"Anna"},{"full_name":"Rodilla, Veronica","last_name":"Rodilla","first_name":"Veronica"},{"full_name":"Huyghe, Mathilde","last_name":"Huyghe","first_name":"Mathilde"},{"full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"},{"last_name":"Landragin","full_name":"Landragin, Camille","first_name":"Camille"},{"first_name":"Olivier","full_name":"Renaud, Olivier","last_name":"Renaud"},{"full_name":"Leroy, Olivier","last_name":"Leroy","first_name":"Olivier"},{"last_name":"Rulands","full_name":"Rulands, Steffen","first_name":"Steffen"},{"first_name":"Benjamin","last_name":"Simons","full_name":"Simons, Benjamin"},{"last_name":"Fré","full_name":"Fré, Silvia","first_name":"Silvia"}],"title":"Clonal analysis of Notch1-expressing cells reveals the existence of unipotent stem cells that retain long-term plasticity in the embryonic mammary gland","citation":{"ista":"Lilja A, Rodilla V, Huyghe M, Hannezo EB, Landragin C, Renaud O, Leroy O, Rulands S, Simons B, Fré S. 2018. Clonal analysis of Notch1-expressing cells reveals the existence of unipotent stem cells that retain long-term plasticity in the embryonic mammary gland. Nature Cell Biology. 20(6), 677–687.","chicago":"Lilja, Anna, Veronica Rodilla, Mathilde Huyghe, Edouard B Hannezo, Camille Landragin, Olivier Renaud, Olivier Leroy, Steffen Rulands, Benjamin Simons, and Silvia Fré. “Clonal Analysis of Notch1-Expressing Cells Reveals the Existence of Unipotent Stem Cells That Retain Long-Term Plasticity in the Embryonic Mammary Gland.” Nature Cell Biology. Nature Publishing Group, 2018. https://doi.org/10.1038/s41556-018-0108-1.","ieee":"A. Lilja et al., “Clonal analysis of Notch1-expressing cells reveals the existence of unipotent stem cells that retain long-term plasticity in the embryonic mammary gland,” Nature Cell Biology, vol. 20, no. 6. Nature Publishing Group, pp. 677–687, 2018.","short":"A. Lilja, V. Rodilla, M. Huyghe, E.B. Hannezo, C. Landragin, O. Renaud, O. Leroy, S. Rulands, B. Simons, S. Fré, Nature Cell Biology 20 (2018) 677–687.","apa":"Lilja, A., Rodilla, V., Huyghe, M., Hannezo, E. B., Landragin, C., Renaud, O., … Fré, S. (2018). Clonal analysis of Notch1-expressing cells reveals the existence of unipotent stem cells that retain long-term plasticity in the embryonic mammary gland. Nature Cell Biology. Nature Publishing Group. https://doi.org/10.1038/s41556-018-0108-1","ama":"Lilja A, Rodilla V, Huyghe M, et al. Clonal analysis of Notch1-expressing cells reveals the existence of unipotent stem cells that retain long-term plasticity in the embryonic mammary gland. Nature Cell Biology. 2018;20(6):677-687. doi:10.1038/s41556-018-0108-1","mla":"Lilja, Anna, et al. “Clonal Analysis of Notch1-Expressing Cells Reveals the Existence of Unipotent Stem Cells That Retain Long-Term Plasticity in the Embryonic Mammary Gland.” Nature Cell Biology, vol. 20, no. 6, Nature Publishing Group, 2018, pp. 677–87, doi:10.1038/s41556-018-0108-1."},"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","oa":1,"quality_controlled":"1","publisher":"Nature Publishing Group","page":"677 - 687","date_created":"2018-12-11T11:45:38Z","doi":"10.1038/s41556-018-0108-1","date_published":"2018-05-21T00:00:00Z","year":"2018","isi":1,"publication":"Nature Cell Biology","day":"21","type":"journal_article","article_type":"original","status":"public","_id":"288","department":[{"_id":"EdHa"}],"date_updated":"2023-09-11T12:44:08Z","main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6984964"}],"scopus_import":"1","intvolume":" 20","month":"05","abstract":[{"text":"Recent lineage tracing studies have revealed that mammary gland homeostasis relies on unipotent stem cells. However, whether and when lineage restriction occurs during embryonic mammary development, and which signals orchestrate cell fate specification, remain unknown. Using a combination of in vivo clonal analysis with whole mount immunofluorescence and mathematical modelling of clonal dynamics, we found that embryonic multipotent mammary cells become lineage-restricted surprisingly early in development, with evidence for unipotency as early as E12.5 and no statistically discernable bipotency after E15.5. To gain insights into the mechanisms governing the switch from multipotency to unipotency, we used gain-of-function Notch1 mice and demonstrated that Notch activation cell autonomously dictates luminal cell fate specification to both embryonic and basally committed mammary cells. These functional studies have important implications for understanding the signals underlying cell plasticity and serve to clarify how reactivation of embryonic programs in adult cells can lead to cancer.","lang":"eng"}],"oa_version":"Submitted Version","pmid":1,"issue":"6","volume":20,"publication_status":"published","language":[{"iso":"eng"}]},{"external_id":{"isi":["000441327300012"]},"article_processing_charge":"No","author":[{"full_name":"Sznurkowska, Magdalena","last_name":"Sznurkowska","first_name":"Magdalena"},{"orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","last_name":"Hannezo","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Azzarelli, Roberta","last_name":"Azzarelli","first_name":"Roberta"},{"first_name":"Steffen","last_name":"Rulands","full_name":"Rulands, Steffen"},{"full_name":"Nestorowa, Sonia","last_name":"Nestorowa","first_name":"Sonia"},{"full_name":"Hindley, Christopher","last_name":"Hindley","first_name":"Christopher"},{"full_name":"Nichols, Jennifer","last_name":"Nichols","first_name":"Jennifer"},{"first_name":"Berthold","last_name":"Göttgens","full_name":"Göttgens, Berthold"},{"full_name":"Huch, Meritxell","last_name":"Huch","first_name":"Meritxell"},{"full_name":"Philpott, Anna","last_name":"Philpott","first_name":"Anna"},{"full_name":"Simons, Benjamin","last_name":"Simons","first_name":"Benjamin"}],"publist_id":"7791","title":"Defining lineage potential and fate behavior of precursors during pancreas development","citation":{"ista":"Sznurkowska M, Hannezo EB, Azzarelli R, Rulands S, Nestorowa S, Hindley C, Nichols J, Göttgens B, Huch M, Philpott A, Simons B. 2018. Defining lineage potential and fate behavior of precursors during pancreas development. Developmental Cell. 46(3), 360–375.","chicago":"Sznurkowska, Magdalena, Edouard B Hannezo, Roberta Azzarelli, Steffen Rulands, Sonia Nestorowa, Christopher Hindley, Jennifer Nichols, et al. “Defining Lineage Potential and Fate Behavior of Precursors during Pancreas Development.” Developmental Cell. Cell Press, 2018. https://doi.org/10.1016/j.devcel.2018.06.028.","ieee":"M. Sznurkowska et al., “Defining lineage potential and fate behavior of precursors during pancreas development,” Developmental Cell, vol. 46, no. 3. Cell Press, pp. 360–375, 2018.","short":"M. Sznurkowska, E.B. Hannezo, R. Azzarelli, S. Rulands, S. Nestorowa, C. Hindley, J. Nichols, B. Göttgens, M. Huch, A. Philpott, B. Simons, Developmental Cell 46 (2018) 360–375.","apa":"Sznurkowska, M., Hannezo, E. B., Azzarelli, R., Rulands, S., Nestorowa, S., Hindley, C., … Simons, B. (2018). Defining lineage potential and fate behavior of precursors during pancreas development. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2018.06.028","ama":"Sznurkowska M, Hannezo EB, Azzarelli R, et al. Defining lineage potential and fate behavior of precursors during pancreas development. Developmental Cell. 2018;46(3):360-375. doi:10.1016/j.devcel.2018.06.028","mla":"Sznurkowska, Magdalena, et al. “Defining Lineage Potential and Fate Behavior of Precursors during Pancreas Development.” Developmental Cell, vol. 46, no. 3, Cell Press, 2018, pp. 360–75, doi:10.1016/j.devcel.2018.06.028."},"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","page":"360 - 375","date_created":"2018-12-11T11:44:48Z","doi":"10.1016/j.devcel.2018.06.028","date_published":"2018-08-06T00:00:00Z","year":"2018","has_accepted_license":"1","isi":1,"publication":"Developmental Cell","day":"06","oa":1,"publisher":"Cell Press","quality_controlled":"1","acknowledgement":"E.H. is funded by a Junior Research Fellowship from Trinity College, Cam-bridge, a Sir Henry Wellcome Fellowship from the Wellcome Trust, and theBettencourt-Schueller Young Researcher Prize for support.","file_date_updated":"2020-07-14T12:44:43Z","department":[{"_id":"EdHa"}],"date_updated":"2023-09-11T12:52:41Z","ddc":["570"],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"type":"journal_article","article_type":"original","status":"public","_id":"132","volume":46,"issue":"3","publication_status":"published","language":[{"iso":"eng"}],"file":[{"checksum":"78d2062b9e3c3b90fe71545aeb6d2f65","file_id":"5694","content_type":"application/pdf","access_level":"open_access","relation":"main_file","date_created":"2018-12-17T10:49:49Z","file_name":"2018_DevelopmentalCell_Sznurkowska.pdf","date_updated":"2020-07-14T12:44:43Z","file_size":8948384,"creator":"dernst"}],"scopus_import":"1","intvolume":" 46","month":"08","abstract":[{"text":"Pancreas development involves a coordinated process in which an early phase of cell segregation is followed by a longer phase of lineage restriction, expansion, and tissue remodeling. By combining clonal tracing and whole-mount reconstruction with proliferation kinetics and single-cell transcriptional profiling, we define the functional basis of pancreas morphogenesis. We show that the large-scale organization of mouse pancreas can be traced to the activity of self-renewing precursors positioned at the termini of growing ducts, which act collectively to drive serial rounds of stochastic ductal bifurcation balanced by termination. During this phase of branching morphogenesis, multipotent precursors become progressively fate-restricted, giving rise to self-renewing acinar-committed precursors that are conveyed with growing ducts, as well as ductal progenitors that expand the trailing ducts and give rise to delaminating endocrine cells. These findings define quantitatively how the functional behavior and lineage progression of precursor pools determine the large-scale patterning of pancreatic sub-compartments.","lang":"eng"}],"oa_version":"Published Version"},{"_id":"5787","type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"status":"public","date_updated":"2023-09-19T09:32:49Z","ddc":["570"],"file_date_updated":"2020-07-14T12:47:11Z","department":[{"_id":"EdHa"}],"abstract":[{"text":"Branching morphogenesis remains a subject of abiding interest. Although much is \r\nknown about the gene regulatory programs and signaling pathways that operate at \r\nthe cellular scale, it has remained unclear how the macroscopic features of branched \r\norgans, including their size, network topology and spatial patterning, are encoded. \r\nLately, it has been proposed that, these features can be explained quantitatively in \r\nseveral organs within a single unifying framework. Based on large-\r\nscale organ recon\r\n-\r\nstructions and cell lineage tracing, it has been argued that morphogenesis follows \r\nfrom the collective dynamics of sublineage- \r\nrestricted self- \r\nrenewing progenitor cells, \r\nlocalized at ductal tips, that act cooperatively to drive a serial process of ductal elon\r\n-\r\ngation and stochastic tip bifurcation. By correlating differentiation or cell cycle exit \r\nwith proximity to maturing ducts, this dynamic results in the specification of a com-\r\nplex network of defined density and statistical organization. These results suggest \r\nthat, for several mammalian tissues, branched epithelial structures develop as a self- \r\norganized process, reliant upon a strikingly simple, but generic, set of local rules, \r\nwithout recourse to a rigid and deterministic sequence of genetically programmed \r\nevents. Here, we review the basis of these findings and discuss their implications.","lang":"eng"}],"oa_version":"Published Version","scopus_import":"1","month":"12","intvolume":" 60","publication_identifier":{"issn":["00121592"]},"file":[{"creator":"dernst","date_updated":"2020-07-14T12:47:11Z","file_size":1313606,"date_created":"2019-02-06T10:40:46Z","file_name":"2018_DevGrowh_Hannezo.pdf","access_level":"open_access","relation":"main_file","content_type":"application/pdf","checksum":"a6d30b0785db902c734a84fecb2eadd9","file_id":"5933"}],"language":[{"iso":"eng"}],"issue":"9","volume":60,"citation":{"ista":"Hannezo EB, Simons BD. 2018. Statistical theory of branching morphogenesis. Development Growth and Differentiation. 60(9), 512–521.","chicago":"Hannezo, Edouard B, and Benjamin D. Simons. “Statistical Theory of Branching Morphogenesis.” Development Growth and Differentiation. Wiley, 2018. https://doi.org/10.1111/dgd.12570.","ama":"Hannezo EB, Simons BD. Statistical theory of branching morphogenesis. Development Growth and Differentiation. 2018;60(9):512-521. doi:10.1111/dgd.12570","apa":"Hannezo, E. B., & Simons, B. D. (2018). Statistical theory of branching morphogenesis. Development Growth and Differentiation. Wiley. https://doi.org/10.1111/dgd.12570","ieee":"E. B. Hannezo and B. D. Simons, “Statistical theory of branching morphogenesis,” Development Growth and Differentiation, vol. 60, no. 9. Wiley, pp. 512–521, 2018.","short":"E.B. Hannezo, B.D. Simons, Development Growth and Differentiation 60 (2018) 512–521.","mla":"Hannezo, Edouard B., and Benjamin D. Simons. “Statistical Theory of Branching Morphogenesis.” Development Growth and Differentiation, vol. 60, no. 9, Wiley, 2018, pp. 512–21, doi:10.1111/dgd.12570."},"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","author":[{"orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"},{"first_name":"Benjamin D.","full_name":"Simons, Benjamin D.","last_name":"Simons"}],"external_id":{"isi":["000453555100002"]},"article_processing_charge":"No","title":"Statistical theory of branching morphogenesis","publisher":"Wiley","quality_controlled":"1","oa":1,"isi":1,"has_accepted_license":"1","year":"2018","day":"09","publication":"Development Growth and Differentiation","page":"512-521","date_published":"2018-12-09T00:00:00Z","doi":"10.1111/dgd.12570","date_created":"2018-12-30T22:59:14Z"},{"abstract":[{"text":"Cell shape is determined by a balance of intrinsic properties of the cell as well as its mechanochemical environment. Inhomogeneous shape changes underlie many morphogenetic events and involve spatial gradients in active cellular forces induced by complex chemical signaling. Here, we introduce a mechanochemical model based on the notion that cell shape changes may be induced by external diffusible biomolecules that influence cellular contractility (or equivalently, adhesions) in a concentration-dependent manner—and whose spatial profile in turn is affected by cell shape. We map out theoretically the possible interplay between chemical concentration and cellular structure. Besides providing a direct route to spatial gradients in cell shape profiles in tissues, we show that the dependence on cell shape helps create robust mechanochemical gradients.","lang":"eng"}],"oa_version":"Submitted Version","main_file_link":[{"url":"https://arxiv.org/abs/1709.01486","open_access":"1"}],"scopus_import":"1","intvolume":" 114","month":"02","publication_status":"published","language":[{"iso":"eng"}],"volume":114,"issue":"4","_id":"421","type":"journal_article","status":"public","date_updated":"2023-09-19T10:13:55Z","department":[{"_id":"EdHa"}],"oa":1,"quality_controlled":"1","publisher":"Biophysical Society","year":"2018","isi":1,"publication":"Biophysical Journal","day":"27","page":"968 - 977","date_created":"2018-12-11T11:46:23Z","doi":"10.1016/j.bpj.2017.12.022","date_published":"2018-02-27T00:00:00Z","citation":{"chicago":"Dasbiswas, Kinjal, Edouard B Hannezo, and Nir Gov. “Theory of Eppithelial Cell Shape Transitions Induced by Mechanoactive Chemical Gradients.” Biophysical Journal. Biophysical Society, 2018. https://doi.org/10.1016/j.bpj.2017.12.022.","ista":"Dasbiswas K, Hannezo EB, Gov N. 2018. Theory of eppithelial cell shape transitions induced by mechanoactive chemical gradients. Biophysical Journal. 114(4), 968–977.","mla":"Dasbiswas, Kinjal, et al. “Theory of Eppithelial Cell Shape Transitions Induced by Mechanoactive Chemical Gradients.” Biophysical Journal, vol. 114, no. 4, Biophysical Society, 2018, pp. 968–77, doi:10.1016/j.bpj.2017.12.022.","apa":"Dasbiswas, K., Hannezo, E. B., & Gov, N. (2018). Theory of eppithelial cell shape transitions induced by mechanoactive chemical gradients. Biophysical Journal. Biophysical Society. https://doi.org/10.1016/j.bpj.2017.12.022","ama":"Dasbiswas K, Hannezo EB, Gov N. Theory of eppithelial cell shape transitions induced by mechanoactive chemical gradients. Biophysical Journal. 2018;114(4):968-977. doi:10.1016/j.bpj.2017.12.022","ieee":"K. Dasbiswas, E. B. Hannezo, and N. Gov, “Theory of eppithelial cell shape transitions induced by mechanoactive chemical gradients,” Biophysical Journal, vol. 114, no. 4. Biophysical Society, pp. 968–977, 2018.","short":"K. Dasbiswas, E.B. Hannezo, N. Gov, Biophysical Journal 114 (2018) 968–977."},"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","article_processing_charge":"No","external_id":{"arxiv":["1709.01486"],"isi":["000428016700021"]},"author":[{"full_name":"Dasbiswas, Kinjal","last_name":"Dasbiswas","first_name":"Kinjal"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Claude-Edouard B","last_name":"Hannezo","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Claude-Edouard B"},{"full_name":"Gov, Nir","last_name":"Gov","first_name":"Nir"}],"publist_id":"7403","title":"Theory of eppithelial cell shape transitions induced by mechanoactive chemical gradients"},{"author":[{"last_name":"Scheele","full_name":"Scheele, Colinda","first_name":"Colinda"},{"orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","last_name":"Hannezo","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Muraro, Mauro","last_name":"Muraro","first_name":"Mauro"},{"last_name":"Zomer","full_name":"Zomer, Anoek","first_name":"Anoek"},{"first_name":"Nathalia","last_name":"Langedijk","full_name":"Langedijk, Nathalia"},{"first_name":"Alexander","full_name":"Van Oudenaarden, Alexander","last_name":"Van Oudenaarden"},{"first_name":"Benjamin","full_name":"Simons, Benjamin","last_name":"Simons"},{"full_name":"Van Rheenen, Jacco","last_name":"Van Rheenen","first_name":"Jacco"}],"publist_id":"6505","title":"Identity and dynamics of mammary stem cells during branching morphogenesis","date_updated":"2021-01-12T08:22:01Z","citation":{"ista":"Scheele C, Hannezo EB, Muraro M, Zomer A, Langedijk N, Van Oudenaarden A, Simons B, Van Rheenen J. 2017. Identity and dynamics of mammary stem cells during branching morphogenesis. Nature. 542(7641), 313–317.","chicago":"Scheele, Colinda, Edouard B Hannezo, Mauro Muraro, Anoek Zomer, Nathalia Langedijk, Alexander Van Oudenaarden, Benjamin Simons, and Jacco Van Rheenen. “Identity and Dynamics of Mammary Stem Cells during Branching Morphogenesis.” Nature. Nature Publishing Group, 2017. https://doi.org/10.1038/nature21046.","ama":"Scheele C, Hannezo EB, Muraro M, et al. Identity and dynamics of mammary stem cells during branching morphogenesis. Nature. 2017;542(7641):313-317. doi:10.1038/nature21046","apa":"Scheele, C., Hannezo, E. B., Muraro, M., Zomer, A., Langedijk, N., Van Oudenaarden, A., … Van Rheenen, J. (2017). Identity and dynamics of mammary stem cells during branching morphogenesis. Nature. Nature Publishing Group. https://doi.org/10.1038/nature21046","ieee":"C. Scheele et al., “Identity and dynamics of mammary stem cells during branching morphogenesis,” Nature, vol. 542, no. 7641. Nature Publishing Group, pp. 313–317, 2017.","short":"C. Scheele, E.B. Hannezo, M. Muraro, A. Zomer, N. Langedijk, A. Van Oudenaarden, B. Simons, J. Van Rheenen, Nature 542 (2017) 313–317.","mla":"Scheele, Colinda, et al. “Identity and Dynamics of Mammary Stem Cells during Branching Morphogenesis.” Nature, vol. 542, no. 7641, Nature Publishing Group, 2017, pp. 313–17, doi:10.1038/nature21046."},"extern":"1","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","type":"journal_article","status":"public","_id":"934","page":"313 - 317","date_published":"2017-02-16T00:00:00Z","doi":"10.1038/nature21046","issue":"7641","volume":542,"date_created":"2018-12-11T11:49:17Z","publication_identifier":{"issn":["00280836"]},"publication_status":"published","year":"2017","day":"16","language":[{"iso":"eng"}],"publication":"Nature","quality_controlled":"1","publisher":"Nature Publishing Group","month":"02","intvolume":" 542","abstract":[{"text":"During puberty, the mouse mammary gland develops into a highly branched epithelial network. Owing to the absence of exclusive stem cell markers, the location, multiplicity, dynamics and fate of mammary stem cells (MaSCs), which drive branching morphogenesis, are unknown. Here we show that morphogenesis is driven by proliferative terminal end buds that terminate or bifurcate with near equal probability, in a stochastic and time-invariant manner, leading to a heterogeneous epithelial network. We show that the majority of terminal end bud cells function as highly proliferative, lineage-committed MaSCs that are heterogeneous in their expression profile and short-term contribution to ductal extension. Yet, through cell rearrangements during terminal end bud bifurcation, each MaSC is able to contribute actively to long-term growth. Our study shows that the behaviour of MaSCs is not directly linked to a single expression profile. Instead, morphogenesis relies upon lineage-restricted heterogeneous MaSC populations that function as single equipotent pools in the long term.","lang":"eng"}],"oa_version":"None"},{"volume":130,"date_published":"2017-01-01T00:00:00Z","doi":"10.1242/jcs.202234","issue":"5","date_created":"2018-12-11T11:49:17Z","day":"01","publication":"Journal of Cell Science","language":[{"iso":"eng"}],"year":"2017","publication_status":"published","month":"01","intvolume":" 130","publisher":"Company of Biologists","quality_controlled":"1","oa_version":"None","abstract":[{"text":"Homeostatic replacement of epithelial cells from basal precursors is a multistep process involving progenitor cell specification, radial intercalation and, finally, apical surface emergence. Recent data demonstrate that actin-based pushing under the control of the formin protein Fmn1 drives apical emergence in nascent multiciliated epithelial cells (MCCs), but little else is known about this actin network or the control of Fmn1. Here, we explore the role of the small GTPase RhoA in MCC apical emergence. Disruption of RhoA function reduced the rate of apical surface expansion and decreased the final size of the apical domain. Analysis of cell shapes suggests that RhoA alters the balance of forces exerted on the MCC apical surface. Finally, quantitative time-lapse imaging and fluorescence recovery after photobleaching studies argue that RhoA works in concert with Fmn1 to control assembly of the specialized apical actin network in MCCs. These data provide new molecular insights into epithelial apical surface assembly and could also shed light on mechanisms of apical lumen formation","lang":"eng"}],"title":"RhoA regulates actin network dynamics during apical surface emergence in multiciliated epithelial cells ","author":[{"full_name":"Sedzinski, Jakub","last_name":"Sedzinski","first_name":"Jakub"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo"},{"first_name":"Fan","last_name":"Tu","full_name":"Tu, Fan"},{"first_name":"Maté","last_name":"Biro","full_name":"Biro, Maté"},{"first_name":"John","full_name":"Wallingford, John","last_name":"Wallingford"}],"publist_id":"6507","extern":"1","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","citation":{"chicago":"Sedzinski, Jakub, Edouard B Hannezo, Fan Tu, Maté Biro, and John Wallingford. “RhoA Regulates Actin Network Dynamics during Apical Surface Emergence in Multiciliated Epithelial Cells .” Journal of Cell Science. Company of Biologists, 2017. https://doi.org/10.1242/jcs.202234.","ista":"Sedzinski J, Hannezo EB, Tu F, Biro M, Wallingford J. 2017. RhoA regulates actin network dynamics during apical surface emergence in multiciliated epithelial cells . Journal of Cell Science. 130(5).","mla":"Sedzinski, Jakub, et al. “RhoA Regulates Actin Network Dynamics during Apical Surface Emergence in Multiciliated Epithelial Cells .” Journal of Cell Science, vol. 130, no. 5, Company of Biologists, 2017, doi:10.1242/jcs.202234.","short":"J. Sedzinski, E.B. Hannezo, F. Tu, M. Biro, J. Wallingford, Journal of Cell Science 130 (2017).","ieee":"J. Sedzinski, E. B. Hannezo, F. Tu, M. Biro, and J. Wallingford, “RhoA regulates actin network dynamics during apical surface emergence in multiciliated epithelial cells ,” Journal of Cell Science, vol. 130, no. 5. Company of Biologists, 2017.","apa":"Sedzinski, J., Hannezo, E. B., Tu, F., Biro, M., & Wallingford, J. (2017). RhoA regulates actin network dynamics during apical surface emergence in multiciliated epithelial cells . Journal of Cell Science. Company of Biologists. https://doi.org/10.1242/jcs.202234","ama":"Sedzinski J, Hannezo EB, Tu F, Biro M, Wallingford J. RhoA regulates actin network dynamics during apical surface emergence in multiciliated epithelial cells . Journal of Cell Science. 2017;130(5). doi:10.1242/jcs.202234"},"date_updated":"2021-01-12T08:22:02Z","status":"public","type":"journal_article","_id":"936"},{"day":"04","language":[{"iso":"eng"}],"publication":"Nature","publication_identifier":{"issn":["00280836"]},"publication_status":"published","year":"2017","doi":"10.1038/nature22041","issue":"7652","date_published":"2017-05-04T00:00:00Z","volume":545,"date_created":"2018-12-11T11:49:18Z","page":"103 - 107","oa_version":"None","abstract":[{"text":"During epithelial cytokinesis, the remodelling of adhesive cell-cell contacts between the dividing cell and its neighbours has profound implications for the integrity, arrangement and morphogenesis of proliferative tissues. In both vertebrates and invertebrates, this remodelling requires the activity of non-muscle myosin II (MyoII) in the interphasic cells neighbouring the dividing cell. However, the mechanisms that coordinate cytokinesis and MyoII activity in the neighbours are unknown. Here we show that in the Drosophila notum epithelium, each cell division is associated with a mechanosensing and transmission event that controls MyoII dynamics in neighbouring cells. We find that the ring pulling forces promote local junction elongation, which results in local E-cadherin dilution at the ingressing adherens junction. In turn, the reduction in E-cadherin concentration and the contractility of the neighbouring cells promote self-organized actomyosin flows, ultimately leading to accumulation of MyoII at the base of the ingressing junction. Although force transduction has been extensively studied in the context of adherens junction reinforcement to stabilize adhesive cell-cell contacts, we propose an alternative mechanosensing mechanism that coordinates actomyosin dynamics between epithelial cells and sustains the remodelling of the adherens junction in response to mechanical forces.","lang":"eng"}],"month":"05","intvolume":" 545","quality_controlled":"1","publisher":"Nature Publishing Group","extern":"1","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","date_updated":"2021-01-12T08:22:02Z","citation":{"mla":"Pinheiro, Diana, et al. “Transmission of Cytokinesis Forces via E Cadherin Dilution and Actomyosin Flows.” Nature, vol. 545, no. 7652, Nature Publishing Group, 2017, pp. 103–07, doi:10.1038/nature22041.","short":"D. Pinheiro, E.B. Hannezo, S. Herszterg, F. Bosveld, I. Gaugué, M. Balakireva, Z. Wang, I. Cristo, S. Rigaud, O. Markova, Y. Bellaïche, Nature 545 (2017) 103–107.","ieee":"D. Pinheiro et al., “Transmission of cytokinesis forces via E cadherin dilution and actomyosin flows,” Nature, vol. 545, no. 7652. Nature Publishing Group, pp. 103–107, 2017.","apa":"Pinheiro, D., Hannezo, E. B., Herszterg, S., Bosveld, F., Gaugué, I., Balakireva, M., … Bellaïche, Y. (2017). Transmission of cytokinesis forces via E cadherin dilution and actomyosin flows. Nature. Nature Publishing Group. https://doi.org/10.1038/nature22041","ama":"Pinheiro D, Hannezo EB, Herszterg S, et al. Transmission of cytokinesis forces via E cadherin dilution and actomyosin flows. Nature. 2017;545(7652):103-107. doi:10.1038/nature22041","chicago":"Pinheiro, Diana, Edouard B Hannezo, Sophie Herszterg, Floris Bosveld, Isabelle Gaugué, Maria Balakireva, Zhimin Wang, et al. “Transmission of Cytokinesis Forces via E Cadherin Dilution and Actomyosin Flows.” Nature. Nature Publishing Group, 2017. https://doi.org/10.1038/nature22041.","ista":"Pinheiro D, Hannezo EB, Herszterg S, Bosveld F, Gaugué I, Balakireva M, Wang Z, Cristo I, Rigaud S, Markova O, Bellaïche Y. 2017. Transmission of cytokinesis forces via E cadherin dilution and actomyosin flows. Nature. 545(7652), 103–107."},"title":"Transmission of cytokinesis forces via E cadherin dilution and actomyosin flows","publist_id":"6504","author":[{"full_name":"Pinheiro, Diana","last_name":"Pinheiro","first_name":"Diana"},{"last_name":"Hannezo","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Sophie","last_name":"Herszterg","full_name":"Herszterg, Sophie"},{"first_name":"Floris","last_name":"Bosveld","full_name":"Bosveld, Floris"},{"first_name":"Isabelle","last_name":"Gaugué","full_name":"Gaugué, Isabelle"},{"first_name":"Maria","full_name":"Balakireva, Maria","last_name":"Balakireva"},{"last_name":"Wang","full_name":"Wang, Zhimin","first_name":"Zhimin"},{"last_name":"Cristo","full_name":"Cristo, Inês","first_name":"Inês"},{"full_name":"Rigaud, Stéphane","last_name":"Rigaud","first_name":"Stéphane"},{"first_name":"Olga","last_name":"Markova","full_name":"Markova, Olga"},{"full_name":"Bellaïche, Yohanns","last_name":"Bellaïche","first_name":"Yohanns"}],"_id":"937","status":"public","type":"journal_article"},{"department":[{"_id":"EdHa"}],"file_date_updated":"2020-07-14T12:47:55Z","ddc":["539"],"date_updated":"2023-09-28T11:34:17Z","status":"public","pubrep_id":"883","type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"_id":"726","volume":171,"issue":"1","file":[{"date_updated":"2020-07-14T12:47:55Z","file_size":12670204,"creator":"system","date_created":"2018-12-12T10:11:17Z","file_name":"IST-2017-883-v1+1_PIIS0092867417309510.pdf","content_type":"application/pdf","access_level":"open_access","relation":"main_file","file_id":"4870","checksum":"7a036d93a9e2e597af9bb504d6133aca"}],"language":[{"iso":"eng"}],"publication_identifier":{"issn":["00928674"]},"publication_status":"published","month":"09","intvolume":" 171","scopus_import":"1","oa_version":"Published Version","abstract":[{"lang":"eng","text":"The morphogenesis of branched organs remains a subject of abiding interest. Although much is known about the underlying signaling pathways, it remains unclear how macroscopic features of branched organs, including their size, network topology, and spatial patterning, are encoded. Here, we show that, in mouse mammary gland, kidney, and human prostate, these features can be explained quantitatively within a single unifying framework of branching and annihilating random walks. Based on quantitative analyses of large-scale organ reconstructions and proliferation kinetics measurements, we propose that morphogenesis follows from the proliferative activity of equipotent tips that stochastically branch and randomly explore their environment but compete neutrally for space, becoming proliferatively inactive when in proximity with neighboring ducts. These results show that complex branched epithelial structures develop as a self-organized process, reliant upon a strikingly simple but generic rule, without recourse to a rigid and deterministic sequence of genetically programmed events."}],"title":"A unifying theory of branching morphogenesis","publist_id":"6952","author":[{"first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561"},{"full_name":"Scheele, Colinda","last_name":"Scheele","first_name":"Colinda"},{"first_name":"Mohammad","full_name":"Moad, Mohammad","last_name":"Moad"},{"full_name":"Drogo, Nicholas","last_name":"Drogo","first_name":"Nicholas"},{"first_name":"Rakesh","full_name":"Heer, Rakesh","last_name":"Heer"},{"full_name":"Sampogna, Rosemary","last_name":"Sampogna","first_name":"Rosemary"},{"first_name":"Jacco","last_name":"Van Rheenen","full_name":"Van Rheenen, Jacco"},{"last_name":"Simons","full_name":"Simons, Benjamin","first_name":"Benjamin"}],"external_id":{"isi":["000411331800024"]},"article_processing_charge":"No","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","citation":{"ista":"Hannezo EB, Scheele C, Moad M, Drogo N, Heer R, Sampogna R, Van Rheenen J, Simons B. 2017. A unifying theory of branching morphogenesis. Cell. 171(1), 242–255.","chicago":"Hannezo, Edouard B, Colinda Scheele, Mohammad Moad, Nicholas Drogo, Rakesh Heer, Rosemary Sampogna, Jacco Van Rheenen, and Benjamin Simons. “A Unifying Theory of Branching Morphogenesis.” Cell. Cell Press, 2017. https://doi.org/10.1016/j.cell.2017.08.026.","short":"E.B. Hannezo, C. Scheele, M. Moad, N. Drogo, R. Heer, R. Sampogna, J. Van Rheenen, B. Simons, Cell 171 (2017) 242–255.","ieee":"E. B. Hannezo et al., “A unifying theory of branching morphogenesis,” Cell, vol. 171, no. 1. Cell Press, pp. 242–255, 2017.","apa":"Hannezo, E. B., Scheele, C., Moad, M., Drogo, N., Heer, R., Sampogna, R., … Simons, B. (2017). A unifying theory of branching morphogenesis. Cell. Cell Press. https://doi.org/10.1016/j.cell.2017.08.026","ama":"Hannezo EB, Scheele C, Moad M, et al. A unifying theory of branching morphogenesis. Cell. 2017;171(1):242-255. doi:10.1016/j.cell.2017.08.026","mla":"Hannezo, Edouard B., et al. “A Unifying Theory of Branching Morphogenesis.” Cell, vol. 171, no. 1, Cell Press, 2017, pp. 242–55, doi:10.1016/j.cell.2017.08.026."},"date_published":"2017-09-21T00:00:00Z","doi":"10.1016/j.cell.2017.08.026","date_created":"2018-12-11T11:48:10Z","page":"242 - 255","day":"21","publication":"Cell","has_accepted_license":"1","isi":1,"year":"2017","publisher":"Cell Press","quality_controlled":"1","oa":1},{"page":"298 - 303","date_created":"2018-12-11T11:49:15Z","issue":"7616","volume":536,"doi":"10.1038/nature19069","date_published":"2016-07-08T00:00:00Z","publication_status":"published","year":"2016","publication":"Nature","language":[{"iso":"eng"}],"day":"08","publisher":"Nature Publishing Group","intvolume":" 536","month":"07","abstract":[{"lang":"eng","text":"The changes in cell dynamics after oncogenic mutation that lead to the development of tumours are currently unknown. Here, using skin epidermis as a model, we assessed the effect of oncogenic hedgehog signalling in distinct cell populations and their capacity to induce basal cell carcinoma, the most frequent cancer in humans. We found that only stem cells, and not progenitors, initiated tumour formation upon oncogenic hedgehog signalling. This difference was due to the hierarchical organization of tumour growth in oncogene-targeted stem cells, characterized by an increase in symmetric self-renewing divisions and a higher p53-dependent resistance to apoptosis, leading to rapid clonal expansion and progression into invasive tumours. Our work reveals that the capacity of oncogene-targeted cells to induce tumour formation is dependent not only on their long-term survival and expansion, but also on the specific clonal dynamics of the cancer cell of origin."}],"acknowledgement":"We would like to thank J.-M. Vanderwinden and the LiMiF for the help with confocal microscopy. C.B. is an investigator of WELBIO. A.S.-D. and J.C.L. are supported by a fellowship of the FNRS and FRIA respectively. B.D.S. and E.H. are supported by the Wellcome Trust (grant numbers 098357/Z/12/Z and 110326/Z/15/Z). E.H. is supported by a fellowship from Trinity College, Cambridge. This work was supported by the FNRS, the IUAP program, the Fondation contre le Cancer, the ULB fondation, the foundation Bettencourt Schueller, the foundation Baillet Latour, a consolidator grant of the European Research Council.","oa_version":"None","article_processing_charge":"No","publist_id":"6508","author":[{"last_name":"Sánchez Danés","full_name":"Sánchez Danés, Adriana","first_name":"Adriana"},{"full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Larsimont, Jean","last_name":"Larsimont","first_name":"Jean"},{"full_name":"Liagre, Mélanie","last_name":"Liagre","first_name":"Mélanie"},{"full_name":"Youssef, Khalil","last_name":"Youssef","first_name":"Khalil"},{"full_name":"Simons, Benjamin","last_name":"Simons","first_name":"Benjamin"},{"first_name":"Cédric","full_name":"Blanpain, Cédric","last_name":"Blanpain"}],"title":"Defining the clonal dynamics leading to mouse skin tumour initiation","citation":{"ista":"Sánchez Danés A, Hannezo EB, Larsimont J, Liagre M, Youssef K, Simons B, Blanpain C. 2016. Defining the clonal dynamics leading to mouse skin tumour initiation. Nature. 536(7616), 298–303.","chicago":"Sánchez Danés, Adriana, Edouard B Hannezo, Jean Larsimont, Mélanie Liagre, Khalil Youssef, Benjamin Simons, and Cédric Blanpain. “Defining the Clonal Dynamics Leading to Mouse Skin Tumour Initiation.” Nature. Nature Publishing Group, 2016. https://doi.org/10.1038/nature19069.","ama":"Sánchez Danés A, Hannezo EB, Larsimont J, et al. Defining the clonal dynamics leading to mouse skin tumour initiation. Nature. 2016;536(7616):298-303. doi:10.1038/nature19069","apa":"Sánchez Danés, A., Hannezo, E. B., Larsimont, J., Liagre, M., Youssef, K., Simons, B., & Blanpain, C. (2016). Defining the clonal dynamics leading to mouse skin tumour initiation. Nature. Nature Publishing Group. https://doi.org/10.1038/nature19069","ieee":"A. Sánchez Danés et al., “Defining the clonal dynamics leading to mouse skin tumour initiation,” Nature, vol. 536, no. 7616. Nature Publishing Group, pp. 298–303, 2016.","short":"A. Sánchez Danés, E.B. Hannezo, J. Larsimont, M. Liagre, K. Youssef, B. Simons, C. Blanpain, Nature 536 (2016) 298–303.","mla":"Sánchez Danés, Adriana, et al. “Defining the Clonal Dynamics Leading to Mouse Skin Tumour Initiation.” Nature, vol. 536, no. 7616, Nature Publishing Group, 2016, pp. 298–303, doi:10.1038/nature19069."},"date_updated":"2021-01-12T08:21:59Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","extern":"1","type":"journal_article","status":"public","_id":"930"},{"type":"journal_article","status":"public","_id":"931","article_processing_charge":"No","publist_id":"6509","author":[{"first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo"},{"first_name":"Alice","last_name":"Coucke","full_name":"Coucke, Alice"},{"first_name":"Jean","last_name":"Joanny","full_name":"Joanny, Jean"}],"title":"Interplay of migratory and division forces as a generic mechanism for stem cell patterns","date_updated":"2021-01-12T08:22:00Z","citation":{"apa":"Hannezo, E. B., Coucke, A., & Joanny, J. (2016). Interplay of migratory and division forces as a generic mechanism for stem cell patterns. Physical Review E Statistical Nonlinear and Soft Matter Physics. American Institute of Physics. https://doi.org/10.1103/PhysRevE.93.022405","ama":"Hannezo EB, Coucke A, Joanny J. Interplay of migratory and division forces as a generic mechanism for stem cell patterns. Physical Review E Statistical Nonlinear and Soft Matter Physics. 2016;93(2). doi:10.1103/PhysRevE.93.022405","ieee":"E. B. Hannezo, A. Coucke, and J. Joanny, “Interplay of migratory and division forces as a generic mechanism for stem cell patterns,” Physical Review E Statistical Nonlinear and Soft Matter Physics, vol. 93, no. 2. American Institute of Physics, 2016.","short":"E.B. Hannezo, A. Coucke, J. Joanny, Physical Review E Statistical Nonlinear and Soft Matter Physics 93 (2016).","mla":"Hannezo, Edouard B., et al. “Interplay of Migratory and Division Forces as a Generic Mechanism for Stem Cell Patterns.” Physical Review E Statistical Nonlinear and Soft Matter Physics, vol. 93, no. 2, American Institute of Physics, 2016, doi:10.1103/PhysRevE.93.022405.","ista":"Hannezo EB, Coucke A, Joanny J. 2016. Interplay of migratory and division forces as a generic mechanism for stem cell patterns. Physical Review E Statistical Nonlinear and Soft Matter Physics. 93(2).","chicago":"Hannezo, Edouard B, Alice Coucke, and Jean Joanny. “Interplay of Migratory and Division Forces as a Generic Mechanism for Stem Cell Patterns.” Physical Review E Statistical Nonlinear and Soft Matter Physics. American Institute of Physics, 2016. https://doi.org/10.1103/PhysRevE.93.022405."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","extern":"1","publisher":"American Institute of Physics","intvolume":" 93","month":"02","abstract":[{"lang":"eng","text":"In many adult tissues, stem cells and differentiated cells are not homogeneously distributed: stem cells are arranged in periodic "niches," and differentiated cells are constantly produced and migrate out of these niches. In this article, we provide a general theoretical framework to study mixtures of dividing and actively migrating particles, which we apply to biological tissues. We show in particular that the interplay between the stresses arising from active cell migration and stem cell division give rise to robust stem cell patterns. The instability of the tissue leads to spatial patterns which are either steady or oscillating in time. The wavelength of the instability has an order of magnitude consistent with the biological observations. We also discuss the implications of these results for future in vitro and in vivo experiments."}],"acknowledgement":"The authors thank Jacques Prost and Pierre Recho for helpful discussions, as well as the Labex CelTisPhyBio and all its members. E.H. acknowledges for funding a Young Researcher Prize from the Bettencourt-Schueller Fondation, and a Junior Research Fellowship from Trinity College, Cambridge.","oa_version":"None","date_created":"2018-12-11T11:49:16Z","doi":"10.1103/PhysRevE.93.022405","volume":93,"date_published":"2016-02-28T00:00:00Z","issue":"2","year":"2016","publication_status":"published","publication":"Physical Review E Statistical Nonlinear and Soft Matter Physics","language":[{"iso":"eng"}],"day":"28"},{"publisher":"Cell Press","month":"01","intvolume":" 36","abstract":[{"text":"Epithelial sheets are crucial components of all metazoan animals, enclosing organs and protecting the animal from its environment. Epithelial homeostasis poses unique challenges, as addition of new cells and loss of old cells must be achieved without disrupting the fluid-tight barrier and apicobasal polarity of the epithelium. Several studies have identified cell biological mechanisms underlying extrusion of cells from epithelia, but far less is known of the converse mechanism by which new cells are added. Here, we combine molecular, pharmacological, and laser-dissection experiments with theoretical modeling to characterize forces driving emergence of an apical surface as single nascent cells are added to a vertebrate epithelium in vivo. We find that this process involves the interplay between cell-autonomous actin-generated pushing forces in the emerging cell and mechanical properties of neighboring cells. Our findings define the forces driving this cell behavior, contributing to a more comprehensive understanding of epithelial homeostasis.","lang":"eng"}],"oa_version":"None","acknowledgement":"We thank J. Bear, B. Goldstein, A. Ewald, and D. Soroldoni for critical reading. This work was funded by an EMBO Long Term Fellowship to J.S., a Research Fellowship from Trinity College, Cambridge and a Bettencourt-Schueller Foundation Young Researcher Prize to E.H., a Cancer Institute NSW Early Career Researcher fellowship (13/ECF/1–25) and a Cancer Australia/Cure Cancer Australia Foundation project grant (1070498) to M.B., and grants from the NHLBI (HL117164) and NIGMS (GM074104) to J.B.W. J.B.W. was an early career scientist of the Howard Hughes Medical Institute. This work was initiated at the New Quantitative Approaches to Morphogenesis Workshop at UCSB, which is funded in part by the National Science Foundation (PHY11-25915) and the NIGMS (GM067110-05).","page":"24 - 35","doi":"10.1016/j.devcel.2015.12.013","date_published":"2016-01-12T00:00:00Z","volume":36,"issue":"1","date_created":"2018-12-11T11:49:16Z","publication_status":"published","year":"2016","day":"12","language":[{"iso":"eng"}],"publication":"Developmental Cell","type":"journal_article","status":"public","_id":"932","author":[{"first_name":"Jakub","last_name":"Sedzinski","full_name":"Sedzinski, Jakub"},{"first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561"},{"first_name":"Fan","last_name":"Tu","full_name":"Tu, Fan"},{"full_name":"Biro, Maté","last_name":"Biro","first_name":"Maté"},{"last_name":"Wallingford","full_name":"Wallingford, John","first_name":"John"}],"publist_id":"6510","article_processing_charge":"No","title":"Emergence of an Apical Epithelial Cell Surface In Vivo","citation":{"ista":"Sedzinski J, Hannezo EB, Tu F, Biro M, Wallingford J. 2016. Emergence of an Apical Epithelial Cell Surface In Vivo. Developmental Cell. 36(1), 24–35.","chicago":"Sedzinski, Jakub, Edouard B Hannezo, Fan Tu, Maté Biro, and John Wallingford. “Emergence of an Apical Epithelial Cell Surface In Vivo.” Developmental Cell. Cell Press, 2016. https://doi.org/10.1016/j.devcel.2015.12.013.","short":"J. Sedzinski, E.B. Hannezo, F. Tu, M. Biro, J. Wallingford, Developmental Cell 36 (2016) 24–35.","ieee":"J. Sedzinski, E. B. Hannezo, F. Tu, M. Biro, and J. Wallingford, “Emergence of an Apical Epithelial Cell Surface In Vivo,” Developmental Cell, vol. 36, no. 1. Cell Press, pp. 24–35, 2016.","apa":"Sedzinski, J., Hannezo, E. B., Tu, F., Biro, M., & Wallingford, J. (2016). Emergence of an Apical Epithelial Cell Surface In Vivo. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2015.12.013","ama":"Sedzinski J, Hannezo EB, Tu F, Biro M, Wallingford J. Emergence of an Apical Epithelial Cell Surface In Vivo. Developmental Cell. 2016;36(1):24-35. doi:10.1016/j.devcel.2015.12.013","mla":"Sedzinski, Jakub, et al. “Emergence of an Apical Epithelial Cell Surface In Vivo.” Developmental Cell, vol. 36, no. 1, Cell Press, 2016, pp. 24–35, doi:10.1016/j.devcel.2015.12.013."},"date_updated":"2021-01-12T08:22:00Z","extern":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"},{"oa_version":"Published Version","abstract":[{"text":"The actomyosin cytoskeleton is a primary force-generating mechanism in morphogenesis, thus a robust spatial control of cytoskeletal positioning is essential. In this report, we demonstrate that actomyosin contractility and planar cell polarity (PCP) interact in post-mitotic Ciona notochord cells to self-assemble and reposition actomyosin rings, which play an essential role for cell elongation. Intriguingly, rings always form at the cells′ anterior edge before migrating towards the center as contractility increases, reflecting a novel dynamical property of the cortex. Our drug and genetic manipulations uncover a tug-of-war between contractility, which localizes cortical flows toward the equator and PCP, which tries to reposition them. We develop a simple model of the physical forces underlying this tug-of-war, which quantitatively reproduces our results. We thus propose a quantitative framework for dissecting the relative contribution of contractility and PCP to the self-assembly and repositioning of cytoskeletal structures, which should be applicable to other morphogenetic events.","lang":"eng"}],"intvolume":" 4","month":"10","language":[{"iso":"eng"}],"file":[{"checksum":"1e4024b3161adcae4a53a0b3dc8a946e","file_id":"5769","content_type":"application/pdf","access_level":"open_access","relation":"main_file","date_created":"2018-12-20T15:50:56Z","file_name":"2015_eLife_Sehring.pdf","date_updated":"2020-07-14T12:48:15Z","file_size":7202224,"creator":"dernst"}],"publication_status":"published","volume":4,"_id":"928","status":"public","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"type":"journal_article","ddc":["539","570"],"extern":"1","date_updated":"2021-01-12T08:21:58Z","file_date_updated":"2020-07-14T12:48:15Z","oa":1,"publisher":"eLife Sciences Publications","quality_controlled":"1","publication":"eLife","day":"21","year":"2015","has_accepted_license":"1","date_created":"2018-12-11T11:49:15Z","doi":"10.7554/eLife.09206","date_published":"2015-10-21T00:00:00Z","article_number":"e09206","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"ista":"Sehring I, Recho P, Denker E, Kourakis M, Mathiesen B, Hannezo EB, Dong B, Jiang D. 2015. Assembly and positioning of actomyosin rings by contractility and planar cell polarity. eLife. 4, e09206.","chicago":"Sehring, Ivonne, Pierre Recho, Elsa Denker, Matthew Kourakis, Birthe Mathiesen, Edouard B Hannezo, Bo Dong, and Di Jiang. “Assembly and Positioning of Actomyosin Rings by Contractility and Planar Cell Polarity.” ELife. eLife Sciences Publications, 2015. https://doi.org/10.7554/eLife.09206.","ama":"Sehring I, Recho P, Denker E, et al. Assembly and positioning of actomyosin rings by contractility and planar cell polarity. eLife. 2015;4. doi:10.7554/eLife.09206","apa":"Sehring, I., Recho, P., Denker, E., Kourakis, M., Mathiesen, B., Hannezo, E. B., … Jiang, D. (2015). Assembly and positioning of actomyosin rings by contractility and planar cell polarity. ELife. eLife Sciences Publications. https://doi.org/10.7554/eLife.09206","ieee":"I. Sehring et al., “Assembly and positioning of actomyosin rings by contractility and planar cell polarity,” eLife, vol. 4. eLife Sciences Publications, 2015.","short":"I. Sehring, P. Recho, E. Denker, M. Kourakis, B. Mathiesen, E.B. Hannezo, B. Dong, D. Jiang, ELife 4 (2015).","mla":"Sehring, Ivonne, et al. “Assembly and Positioning of Actomyosin Rings by Contractility and Planar Cell Polarity.” ELife, vol. 4, e09206, eLife Sciences Publications, 2015, doi:10.7554/eLife.09206."},"title":"Assembly and positioning of actomyosin rings by contractility and planar cell polarity","publist_id":"6512","author":[{"first_name":"Ivonne","full_name":"Sehring, Ivonne","last_name":"Sehring"},{"last_name":"Recho","full_name":"Recho, Pierre","first_name":"Pierre"},{"first_name":"Elsa","full_name":"Denker, Elsa","last_name":"Denker"},{"first_name":"Matthew","last_name":"Kourakis","full_name":"Kourakis, Matthew"},{"last_name":"Mathiesen","full_name":"Mathiesen, Birthe","first_name":"Birthe"},{"last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"},{"full_name":"Dong, Bo","last_name":"Dong","first_name":"Bo"},{"first_name":"Di","full_name":"Jiang, Di","last_name":"Jiang"}]},{"intvolume":" 76","month":"02","publisher":"Elsevier","acknowledgement":"The work presented in this paper is supported by Alstom Transport, site de Tarbes (Contract number is 11099).","oa_version":"None","abstract":[{"text":"This paper presents a numerical study of a Capillary Pumped Loop evaporator. A two-dimensional unsteady mathematical model of a flat evaporator is developed to simulate heat and mass transfer in unsaturated porous wick with phase change. The liquid-vapor phase change inside the porous wick is described by Langmuir's law. The governing equations are solved by the Finite Element Method. The results are presented then for a sintered nickel wick and methanol as a working fluid. The heat flux required to the transition from the all-liquid wick to the vapor-liquid wick is calculated. The dynamic and thermodynamic behavior of the working fluid in the capillary structure are discussed in this paper.","lang":"eng"}],"date_created":"2018-12-11T11:49:13Z","doi":"10.1016/j.applthermaleng.2014.10.009","date_published":"2015-02-05T00:00:00Z","volume":76,"page":"1 - 8","publication":"Applied Thermal Engineering","language":[{"iso":"eng"}],"day":"05","publication_status":"published","year":"2015","status":"public","type":"journal_article","_id":"924","title":"Dynamic model of heat and mass transfer in an unsaturated porous wick of capillary pumped loop","article_processing_charge":"No","author":[{"first_name":"Riadh","full_name":"Boubaker, Riadh","last_name":"Boubaker"},{"last_name":"Platel","full_name":"Platel, Vincent","first_name":"Vincent"},{"last_name":"Bergès","full_name":"Bergès, Alexis","first_name":"Alexis"},{"last_name":"Bancelin","full_name":"Bancelin, Mathieu","first_name":"Mathieu"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","last_name":"Hannezo"}],"publist_id":"6514","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","extern":"1","date_updated":"2021-01-12T08:21:56Z","citation":{"chicago":"Boubaker, Riadh, Vincent Platel, Alexis Bergès, Mathieu Bancelin, and Edouard B Hannezo. “Dynamic Model of Heat and Mass Transfer in an Unsaturated Porous Wick of Capillary Pumped Loop.” Applied Thermal Engineering. Elsevier, 2015. https://doi.org/10.1016/j.applthermaleng.2014.10.009.","ista":"Boubaker R, Platel V, Bergès A, Bancelin M, Hannezo EB. 2015. Dynamic model of heat and mass transfer in an unsaturated porous wick of capillary pumped loop. Applied Thermal Engineering. 76, 1–8.","mla":"Boubaker, Riadh, et al. “Dynamic Model of Heat and Mass Transfer in an Unsaturated Porous Wick of Capillary Pumped Loop.” Applied Thermal Engineering, vol. 76, Elsevier, 2015, pp. 1–8, doi:10.1016/j.applthermaleng.2014.10.009.","ieee":"R. Boubaker, V. Platel, A. Bergès, M. Bancelin, and E. B. Hannezo, “Dynamic model of heat and mass transfer in an unsaturated porous wick of capillary pumped loop,” Applied Thermal Engineering, vol. 76. Elsevier, pp. 1–8, 2015.","short":"R. Boubaker, V. Platel, A. Bergès, M. Bancelin, E.B. Hannezo, Applied Thermal Engineering 76 (2015) 1–8.","apa":"Boubaker, R., Platel, V., Bergès, A., Bancelin, M., & Hannezo, E. B. (2015). Dynamic model of heat and mass transfer in an unsaturated porous wick of capillary pumped loop. Applied Thermal Engineering. Elsevier. https://doi.org/10.1016/j.applthermaleng.2014.10.009","ama":"Boubaker R, Platel V, Bergès A, Bancelin M, Hannezo EB. Dynamic model of heat and mass transfer in an unsaturated porous wick of capillary pumped loop. Applied Thermal Engineering. 2015;76:1-8. doi:10.1016/j.applthermaleng.2014.10.009"}},{"acknowledgement":"We thank H. Oda, R. E. Ward, K. Saigo, T. Nishimura, D. Pinheiro, Y. Bellaiche, the Bloomington Stock Center, Drosophila Genetic Resource Center (Kyoto), and the Developmental Studies Hybridoma Bank for generously providing antibodies and fly stocks; A. Hayashi for sharing phalloidin staining samples; Y. H. Zhang for plasmid and protocol for CBP preparation; and T. Kondo and J. Prost for suggestions and discussion. This work was supported by the Taishan Scholar Program of Shandong and the Fundamental Research Funds for the Central Universities in China (3005000-841412019) (to B.D.) and a Grant-in-Aid for Scientific Research on Innovative Areas from Ministry of Education, Culture, Sports, Science and Technology of Japan (to S.H.). E.H. acknowledges support from the Young Researcher Prize of the Bettencourt-Schueller Foundation.","oa_version":"None","abstract":[{"text":"An essential question of morphogenesis is how patterns arise without preexisting positional information, as inspired by Turing. In the past few years, cytoskeletal flows in the cell cortex have been identified as a key mechanism of molecular patterning at the subcellular level. Theoretical and in vitro studies have suggested that biological polymers such as actomyosin gels have the property to self-organize, but the applicability of this concept in an in vivo setting remains unclear. Here, we report that the regular spacing pattern of supracellular actin rings in the Drosophila tracheal tubule is governed by a self-organizing principle. We propose a simple biophysical model where pattern formation arises from the interplay of myosin contractility and actin turnover. We validate the hypotheses of the model using photobleaching experiments and report that the formation of actin rings is contractility dependent. Moreover, genetic and pharmacological perturbations of the physical properties of the actomyosin gel modify the spacing of the pattern, as the model predicted. In addition, our model posited a role of cortical friction in stabilizing the spacing pattern of actin rings. Consistently, genetic depletion of apical extracellular matrix caused strikingly dynamic movements of actin rings, mirroring our model prediction of a transition from steady to chaotic actin patterns at low cortical friction. Our results therefore demonstrate quantitatively that a hydrodynamical instability of the actin cortex can trigger regular pattern formation and drive morphogenesis in an in vivo setting. ","lang":"eng"}],"intvolume":" 112","month":"07","publisher":"National Academy of Sciences","publication":"PNAS","language":[{"iso":"eng"}],"day":"14","year":"2015","publication_status":"published","date_created":"2018-12-11T11:49:15Z","doi":"10.1073/pnas.1504762112","date_published":"2015-07-14T00:00:00Z","volume":112,"issue":"28","page":"8620 - 8625","_id":"929","status":"public","type":"journal_article","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","extern":"1","date_updated":"2021-01-12T08:21:59Z","citation":{"mla":"Hannezo, Edouard B., et al. “Cortical Instability Drives Periodic Supracellular Actin Pattern Formation in Epithelial Tubes.” PNAS, vol. 112, no. 28, National Academy of Sciences, 2015, pp. 8620–25, doi:10.1073/pnas.1504762112.","ieee":"E. B. Hannezo, B. Dong, P. Recho, J. Joanny, and S. Hayashi, “Cortical instability drives periodic supracellular actin pattern formation in epithelial tubes,” PNAS, vol. 112, no. 28. National Academy of Sciences, pp. 8620–8625, 2015.","short":"E.B. Hannezo, B. Dong, P. Recho, J. Joanny, S. Hayashi, PNAS 112 (2015) 8620–8625.","ama":"Hannezo EB, Dong B, Recho P, Joanny J, Hayashi S. Cortical instability drives periodic supracellular actin pattern formation in epithelial tubes. PNAS. 2015;112(28):8620-8625. doi:10.1073/pnas.1504762112","apa":"Hannezo, E. B., Dong, B., Recho, P., Joanny, J., & Hayashi, S. (2015). Cortical instability drives periodic supracellular actin pattern formation in epithelial tubes. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1504762112","chicago":"Hannezo, Edouard B, Bo Dong, Pierre Recho, Jean Joanny, and Shigeo Hayashi. “Cortical Instability Drives Periodic Supracellular Actin Pattern Formation in Epithelial Tubes.” PNAS. National Academy of Sciences, 2015. https://doi.org/10.1073/pnas.1504762112.","ista":"Hannezo EB, Dong B, Recho P, Joanny J, Hayashi S. 2015. Cortical instability drives periodic supracellular actin pattern formation in epithelial tubes. PNAS. 112(28), 8620–8625."},"title":"Cortical instability drives periodic supracellular actin pattern formation in epithelial tubes","article_processing_charge":"No","author":[{"last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"},{"first_name":"Bo","full_name":"Dong, Bo","last_name":"Dong"},{"last_name":"Recho","full_name":"Recho, Pierre","first_name":"Pierre"},{"first_name":"Jean","last_name":"Joanny","full_name":"Joanny, Jean"},{"full_name":"Hayashi, Shigeo","last_name":"Hayashi","first_name":"Shigeo"}],"publist_id":"6513"},{"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"mla":"García, Simón, et al. “Physics of Active Jamming during Collective Cellular Motion in a Monolayer.” PNAS, vol. 112, no. 50, National Academy of Sciences, 2015, pp. 15314–19, doi:10.1073/pnas.1510973112.","apa":"García, S., Hannezo, E. B., Elgeti, J., Joanny, J., Silberzan, P., & Gov, N. (2015). Physics of active jamming during collective cellular motion in a monolayer. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1510973112","ama":"García S, Hannezo EB, Elgeti J, Joanny J, Silberzan P, Gov N. Physics of active jamming during collective cellular motion in a monolayer. PNAS. 2015;112(50):15314-15319. doi:10.1073/pnas.1510973112","short":"S. García, E.B. Hannezo, J. Elgeti, J. Joanny, P. Silberzan, N. Gov, PNAS 112 (2015) 15314–15319.","ieee":"S. García, E. B. Hannezo, J. Elgeti, J. Joanny, P. Silberzan, and N. Gov, “Physics of active jamming during collective cellular motion in a monolayer,” PNAS, vol. 112, no. 50. National Academy of Sciences, pp. 15314–15319, 2015.","chicago":"García, Simón, Edouard B Hannezo, Jens Elgeti, Jean Joanny, Pascal Silberzan, and Nir Gov. “Physics of Active Jamming during Collective Cellular Motion in a Monolayer.” PNAS. National Academy of Sciences, 2015. https://doi.org/10.1073/pnas.1510973112.","ista":"García S, Hannezo EB, Elgeti J, Joanny J, Silberzan P, Gov N. 2015. Physics of active jamming during collective cellular motion in a monolayer. PNAS. 112(50), 15314–15319."},"title":"Physics of active jamming during collective cellular motion in a monolayer","publist_id":"6511","author":[{"first_name":"Simón","last_name":"García","full_name":"García, Simón"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo"},{"full_name":"Elgeti, Jens","last_name":"Elgeti","first_name":"Jens"},{"first_name":"Jean","last_name":"Joanny","full_name":"Joanny, Jean"},{"last_name":"Silberzan","full_name":"Silberzan, Pascal","first_name":"Pascal"},{"full_name":"Gov, Nir","last_name":"Gov","first_name":"Nir"}],"external_id":{"pmid":["26627719"]},"day":"15","publication":"PNAS","year":"2015","date_published":"2015-12-15T00:00:00Z","doi":"10.1073/pnas.1510973112","date_created":"2018-12-11T11:49:16Z","page":"15314 - 15319","quality_controlled":"1","publisher":"National Academy of Sciences","oa":1,"extern":"1","date_updated":"2021-01-12T08:22:01Z","_id":"933","status":"public","type":"journal_article","language":[{"iso":"eng"}],"publication_status":"published","issue":"50","volume":112,"oa_version":"None","pmid":1,"abstract":[{"text":"Although collective cell motion plays an important role, for example during wound healing, embryogenesis, or cancer progression, the fundamental rules governing this motion are still not well understood, in particular at high cell density. We study here the motion of human bronchial epithelial cells within a monolayer, over long times. We observe that, as the monolayer ages, the cells slow down monotonously, while the velocity correlation length first increases as the cells slow down but eventually decreases at the slowest motions. By comparing experiments, analytic model, and detailed particle-based simulations, we shed light on this biological amorphous solidification process, demonstrating that the observed dynamics can be explained as a consequence of the combined maturation and strengthening of cell-cell and cell-substrate adhesions. Surprisingly, the increase of cell surface density due to proliferation is only secondary in this process. This analysis is confirmed with two other cell types. The very general relations between the mean cell velocity and velocity correlation lengths, which apply for aggregates of self-propelled particles, as well as motile cells, can possibly be used to discriminate between various parameter changes in vivo, from noninvasive microscopy data.","lang":"eng"}],"month":"12","intvolume":" 112","main_file_link":[{"open_access":"1","url":"https://www.pnas.org/content/pnas/112/50/15314.full.pdf"}]},{"_id":"925","type":"journal_article","status":"public","date_updated":"2021-01-12T08:21:57Z","citation":{"ista":"Dong B, Hannezo EB, Hayashi S. 2014. Balance between apical membrane growth and luminal matrix resistance determines epithelial tubule shape. Cell Reports. 7(4), 941–950.","chicago":"Dong, Bo, Edouard B Hannezo, and Shigeo Hayashi. “Balance between Apical Membrane Growth and Luminal Matrix Resistance Determines Epithelial Tubule Shape.” Cell Reports. Cell Press, 2014. https://doi.org/10.1016/j.celrep.2014.03.066.","ieee":"B. Dong, E. B. Hannezo, and S. Hayashi, “Balance between apical membrane growth and luminal matrix resistance determines epithelial tubule shape,” Cell Reports, vol. 7, no. 4. Cell Press, pp. 941–950, 2014.","short":"B. Dong, E.B. Hannezo, S. Hayashi, Cell Reports 7 (2014) 941–950.","ama":"Dong B, Hannezo EB, Hayashi S. Balance between apical membrane growth and luminal matrix resistance determines epithelial tubule shape. Cell Reports. 2014;7(4):941-950. doi:10.1016/j.celrep.2014.03.066","apa":"Dong, B., Hannezo, E. B., & Hayashi, S. (2014). Balance between apical membrane growth and luminal matrix resistance determines epithelial tubule shape. Cell Reports. Cell Press. https://doi.org/10.1016/j.celrep.2014.03.066","mla":"Dong, Bo, et al. “Balance between Apical Membrane Growth and Luminal Matrix Resistance Determines Epithelial Tubule Shape.” Cell Reports, vol. 7, no. 4, Cell Press, 2014, pp. 941–50, doi:10.1016/j.celrep.2014.03.066."},"extern":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"last_name":"Dong","full_name":"Dong, Bo","first_name":"Bo"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","last_name":"Hannezo"},{"first_name":"Shigeo","full_name":"Hayashi, Shigeo","last_name":"Hayashi"}],"publist_id":"6515","article_processing_charge":"No","title":"Balance between apical membrane growth and luminal matrix resistance determines epithelial tubule shape","abstract":[{"lang":"eng","text":"The morphological stability of biological tubes is crucial for the efficient circulation of fluids and gases. Failure of this stability causes irregularly shaped tubes found in multiple pathological conditions. Here, we report that Drosophila mutants of the ESCRT III component Shrub/Vps32 exhibit a strikingly elongated sinusoidal tube phenotype. This is caused by excessive apical membrane synthesis accompanied by the ectopic accumulation and overactivation of Crumbs in swollen endosomes. Furthermore, we demonstrate that the apical extracellular matrix (aECM) of the tracheal tube is a viscoelastic material coupled with the apical membrane. We present a simple mechanical model in which aECM elasticity, apical membrane growth, and their interaction are three vital parameters determining the stability of biological tubes. Our findings demonstrate a mechanical role for the extracellular matrix and suggest that the interaction of the apical membrane and an elastic aECM determines the final morphology of biological tubes independent of cell shape."}],"oa_version":"None","acknowledgement":"We thank F. Gao, R.E. Ward, S. Luschnig, T. Okajima, M. Affolter, D. Bilder, E. Knust, T. Tanaka, A. Nakamura, C. Samakovlis, K. Saigo, M. Furuse, the Bloomington Stock Center, Drosophila Genetic Resource Center in Kyoto, Japan, and the Developmental Studies Hybridoma Bank for generously providing antibodies and fly stocks; H. Wada for UAS-3×TagRFP fly and dye injection; Y.H. Zhang for plasmid and protocol for CBP preparation; and J. Prost and J.F. Joanny for their support for the project and discussion. We also thank T. Shibata, Y. Morishita, T. Kondo, and G. Sheng for critically reading the manuscript. This work was supported by a Grant-in-Aid for Scientific Research on Innovative Areas from MEXT Japan to S.H. and the RIKEN Foreign Postdoctoral Researcher Program to B.D.","publisher":"Cell Press","month":"05","intvolume":" 7","year":"2014","publication_status":"published","day":"22","publication":"Cell Reports","language":[{"iso":"eng"}],"page":"941 - 950","date_published":"2014-05-22T00:00:00Z","volume":7,"issue":"4","doi":"10.1016/j.celrep.2014.03.066","date_created":"2018-12-11T11:49:14Z"},{"_id":"927","status":"public","type":"journal_article","extern":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"chicago":"Hannezo, Edouard B, Jacques Prost, and Jean Joanny. “Theory of Epithelial Sheet Morphology in Three Dimensions.” PNAS. National Academy of Sciences, 2014. https://doi.org/10.1073/pnas.1312076111.","ista":"Hannezo EB, Prost J, Joanny J. 2014. Theory of epithelial sheet morphology in three dimensions. PNAS. 111(1), 27–32.","mla":"Hannezo, Edouard B., et al. “Theory of Epithelial Sheet Morphology in Three Dimensions.” PNAS, vol. 111, no. 1, National Academy of Sciences, 2014, pp. 27–32, doi:10.1073/pnas.1312076111.","ieee":"E. B. Hannezo, J. Prost, and J. Joanny, “Theory of epithelial sheet morphology in three dimensions,” PNAS, vol. 111, no. 1. National Academy of Sciences, pp. 27–32, 2014.","short":"E.B. Hannezo, J. Prost, J. Joanny, PNAS 111 (2014) 27–32.","ama":"Hannezo EB, Prost J, Joanny J. Theory of epithelial sheet morphology in three dimensions. PNAS. 2014;111(1):27-32. doi:10.1073/pnas.1312076111","apa":"Hannezo, E. B., Prost, J., & Joanny, J. (2014). Theory of epithelial sheet morphology in three dimensions. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1312076111"},"date_updated":"2021-01-12T08:21:58Z","title":"Theory of epithelial sheet morphology in three dimensions","publist_id":"6517","author":[{"first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","last_name":"Hannezo"},{"first_name":"Jacques","last_name":"Prost","full_name":"Prost, Jacques"},{"last_name":"Joanny","full_name":"Joanny, Jean","first_name":"Jean"}],"article_processing_charge":"No","oa_version":"None","abstract":[{"lang":"eng","text":"Morphogenesis during embryo development requires the coordination of mechanical forces to generate the macroscopic shapes of organs. We propose a minimal theoretical model, based on cell adhesion and actomyosin contractility, which describes the various shapes of epithelial cells and the bending and buckling of epithelial sheets, as well as the relative stability of cellular tubes and spheres. We show that, to understand these processes, a full 3D description of the cells is needed, but that simple scaling laws can still be derived. The morphologies observed in vivo can be understood as stable points of mechanical equations and the transitions between them are either continuous or discontinuous. We then focus on epithelial sheet bending, a ubiquitous morphogenetic process. We calculate the curvature of an epithelium as a function of actin belt tension as well as of cell-cell and and cell-substrate tension. The model allows for a comparison of the relative stabilities of spherical or cylindrical cellular structures (acini or tubes). Finally, we propose a unique type of buckling instability of epithelia, driven by a flattening of individual cell shapes, and discuss experimental tests to verify our predictions."}],"month":"01","intvolume":" 111","publisher":"National Academy of Sciences","day":"01","publication":"PNAS","language":[{"iso":"eng"}],"year":"2014","publication_status":"published","volume":111,"date_published":"2014-01-01T00:00:00Z","doi":"10.1073/pnas.1312076111","issue":"1","date_created":"2018-12-11T11:49:14Z","page":"27 - 32"},{"intvolume":" 11","month":"04","publisher":"Royal Society of London","acknowledgement":"We thank Jens Elgeti and Silvia Fre for fruitful discussions.","oa_version":"None","abstract":[{"text":"The regulation of cell growth in animal tissues is a question of critical importance: most tissues contain different types of cells in interconversion and the fraction of each type has to be controlled in a precise way, by mechanisms that remain unclear. Here, we provide a theoretical framework for the homeostasis of stem-cell-containing epithelial tissues using mechanical equations, which describe the size of the tissue and kinetic equations, which describe the interconversions of the cell populations. We show that several features, such as the evolution of stem cell fractions during intestinal development, the shape of a developing intestinal wall, as well as the increase in the proliferative compartment in cancer initiation, can be studied and understood from generic modelling which does not rely on a particular regulatory mechanism. Finally, inspired by recent experiments, we propose a model where cell division rates are regulated by the mechanical stresses in the epithelial sheet. We show that pressure-controlled growth can, in addition to the previous features, also explain with few parameters the formation of stem cell compartments as well as the morphologies observed when a colonic crypt becomes cancerous. We also discuss optimal strategies of wound healing, in connection with experiments on the cornea.","lang":"eng"}],"date_created":"2018-12-11T11:49:14Z","date_published":"2014-04-06T00:00:00Z","doi":"10.1098/rsif.2013.0895","issue":"93","volume":11,"language":[{"iso":"eng"}],"publication":"Journal of the Royal Society Interface","day":"06","publication_status":"published","year":"2014","status":"public","type":"journal_article","_id":"926","title":"Growth homeostatic regulation and stem cell dynamics in tissues","article_processing_charge":"No","author":[{"last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"},{"first_name":"Jacques","full_name":"Prost, Jacques","last_name":"Prost"},{"first_name":"Jean","last_name":"Joanny","full_name":"Joanny, Jean"}],"publist_id":"6516","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","extern":"1","date_updated":"2021-01-12T08:21:57Z","citation":{"chicago":"Hannezo, Edouard B, Jacques Prost, and Jean Joanny. “Growth Homeostatic Regulation and Stem Cell Dynamics in Tissues.” Journal of the Royal Society Interface. Royal Society of London, 2014. https://doi.org/10.1098/rsif.2013.0895.","ista":"Hannezo EB, Prost J, Joanny J. 2014. Growth homeostatic regulation and stem cell dynamics in tissues. Journal of the Royal Society Interface. 11(93).","mla":"Hannezo, Edouard B., et al. “Growth Homeostatic Regulation and Stem Cell Dynamics in Tissues.” Journal of the Royal Society Interface, vol. 11, no. 93, Royal Society of London, 2014, doi:10.1098/rsif.2013.0895.","ieee":"E. B. Hannezo, J. Prost, and J. Joanny, “Growth homeostatic regulation and stem cell dynamics in tissues,” Journal of the Royal Society Interface, vol. 11, no. 93. Royal Society of London, 2014.","short":"E.B. Hannezo, J. Prost, J. Joanny, Journal of the Royal Society Interface 11 (2014).","ama":"Hannezo EB, Prost J, Joanny J. Growth homeostatic regulation and stem cell dynamics in tissues. Journal of the Royal Society Interface. 2014;11(93). doi:10.1098/rsif.2013.0895","apa":"Hannezo, E. B., Prost, J., & Joanny, J. (2014). Growth homeostatic regulation and stem cell dynamics in tissues. Journal of the Royal Society Interface. Royal Society of London. https://doi.org/10.1098/rsif.2013.0895"}},{"abstract":[{"lang":"eng","text":"Recent experiments have shown that spreading epithelial sheets exhibit a long-range coordination of motility forces that leads to a buildup of tension in the tissue, which may enhance cell division and the speed of wound healing. Furthermore, the edges of these epithelial sheets commonly show finger-like protrusions whereas the bulk often displays spontaneous swirls of motile cells. To explain these experimental observations, we propose a simple flocking-type mechanism, in which cells tend to align their motility forceswith their velocity. Implementing this idea in amechanical tissue simulation, the proposed model gives rise to efficient spreading and can explain the experimentally observed long-range alignment of motility forces in highly disordered patterns, as well as the buildup of tensile stress throughout the tissue. Our model also qualitatively reproduces the dependence of swirl size and swirl velocity on cell density reported in experiments and exhibits an undulation instability at the edge of the spreading tissue commonly observed in vivo. Finally, we study the dependence of colony spreading speed on important physical and biological parameters and derive simple scaling relations that show that coordination of motility forces leads to an improvement of the wound healing process for realistic tissue parameters."}],"oa_version":"None","acknowledgement":"This work was supported by National Science Foundation (NSF) Grant DMS-1068869 and by the NSF Center for Theoretical Biological Physics (Grant NSF PHY-0822283).\r\nWe acknowledge useful discussions with Eshel Ben-Jacob and Assaf Zaritsky. ","publisher":"National Academy of Sciences","month":"02","intvolume":" 110","year":"2013","publication_status":"published","day":"12","language":[{"iso":"eng"}],"publication":"PNAS","page":"2452 - 2459","doi":"10.1073/pnas.1219937110","date_published":"2013-02-12T00:00:00Z","volume":110,"issue":"7","date_created":"2018-12-11T11:49:12Z","_id":"921","type":"journal_article","status":"public","date_updated":"2021-01-12T08:21:55Z","citation":{"short":"M. Basan, J. Elgeti, E.B. Hannezo, W. Rappel, H. Levine, PNAS 110 (2013) 2452–2459.","ieee":"M. Basan, J. Elgeti, E. B. Hannezo, W. Rappel, and H. Levine, “Alignment of cellular motility forces with tissue flow as a mechanism for efficient wound healing,” PNAS, vol. 110, no. 7. National Academy of Sciences, pp. 2452–2459, 2013.","ama":"Basan M, Elgeti J, Hannezo EB, Rappel W, Levine H. Alignment of cellular motility forces with tissue flow as a mechanism for efficient wound healing. PNAS. 2013;110(7):2452-2459. doi:10.1073/pnas.1219937110","apa":"Basan, M., Elgeti, J., Hannezo, E. B., Rappel, W., & Levine, H. (2013). Alignment of cellular motility forces with tissue flow as a mechanism for efficient wound healing. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1219937110","mla":"Basan, Markus, et al. “Alignment of Cellular Motility Forces with Tissue Flow as a Mechanism for Efficient Wound Healing.” PNAS, vol. 110, no. 7, National Academy of Sciences, 2013, pp. 2452–59, doi:10.1073/pnas.1219937110.","ista":"Basan M, Elgeti J, Hannezo EB, Rappel W, Levine H. 2013. Alignment of cellular motility forces with tissue flow as a mechanism for efficient wound healing. PNAS. 110(7), 2452–2459.","chicago":"Basan, Markus, Jens Elgeti, Edouard B Hannezo, Wouter Rappel, and Herbert Levine. “Alignment of Cellular Motility Forces with Tissue Flow as a Mechanism for Efficient Wound Healing.” PNAS. National Academy of Sciences, 2013. https://doi.org/10.1073/pnas.1219937110."},"extern":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"first_name":"Markus","last_name":"Basan","full_name":"Basan, Markus"},{"first_name":"Jens","last_name":"Elgeti","full_name":"Elgeti, Jens"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo"},{"full_name":"Rappel, Wouter","last_name":"Rappel","first_name":"Wouter"},{"first_name":"Herbert","last_name":"Levine","full_name":"Levine, Herbert"}],"publist_id":"6518","article_processing_charge":"No","title":"Alignment of cellular motility forces with tissue flow as a mechanism for efficient wound healing"},{"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","extern":"1","date_updated":"2021-01-12T08:21:56Z","citation":{"ista":"Hannezo EB, Prost J, Joanny J. 2012. Mechanical instabilities of biological tubes. Physical Review Letters. 109(1).","chicago":"Hannezo, Edouard B, Jacques Prost, and Jean Joanny. “Mechanical Instabilities of Biological Tubes.” Physical Review Letters. American Physical Society, 2012. https://doi.org/10.1103/PhysRevLett.109.018101.","ama":"Hannezo EB, Prost J, Joanny J. Mechanical instabilities of biological tubes. Physical Review Letters. 2012;109(1). doi:10.1103/PhysRevLett.109.018101","apa":"Hannezo, E. B., Prost, J., & Joanny, J. (2012). Mechanical instabilities of biological tubes. Physical Review Letters. American Physical Society. https://doi.org/10.1103/PhysRevLett.109.018101","ieee":"E. B. Hannezo, J. Prost, and J. Joanny, “Mechanical instabilities of biological tubes,” Physical Review Letters, vol. 109, no. 1. American Physical Society, 2012.","short":"E.B. Hannezo, J. Prost, J. Joanny, Physical Review Letters 109 (2012).","mla":"Hannezo, Edouard B., et al. “Mechanical Instabilities of Biological Tubes.” Physical Review Letters, vol. 109, no. 1, American Physical Society, 2012, doi:10.1103/PhysRevLett.109.018101."},"title":"Mechanical instabilities of biological tubes","article_processing_charge":"No","external_id":{"arxiv":["1207.1516"]},"publist_id":"6519","author":[{"orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"},{"first_name":"Jacques","full_name":"Prost, Jacques","last_name":"Prost"},{"full_name":"Joanny, Jean","last_name":"Joanny","first_name":"Jean"}],"_id":"922","status":"public","type":"journal_article","publication":"Physical Review Letters","language":[{"iso":"eng"}],"day":"03","publication_status":"published","year":"2012","date_created":"2018-12-11T11:49:13Z","volume":109,"doi":"10.1103/PhysRevLett.109.018101","date_published":"2012-07-03T00:00:00Z","issue":"1","oa_version":"Preprint","abstract":[{"lang":"eng","text":"We study theoretically the morphologies of biological tubes affected by various pathologies. When epithelial cells grow, the negative tension produced by their division provokes a buckling instability. Several shapes are investigated: varicose, dilated, sinuous, or sausagelike. They are all found in pathologies of tracheal, renal tubes, or arteries. The final shape depends crucially on the mechanical parameters of the tissues: Young's modulus, wall-to-lumen ratio, homeostatic pressure. We argue that since tissues must be in quasistatic mechanical equilibrium, abnormal shapes convey information as to what causes the pathology. We calculate a phase diagram of tubular instabilities which could be a helpful guide for investigating the underlying genetic regulation."}],"intvolume":" 109","month":"07","main_file_link":[{"open_access":"1","url":"https://arxiv.org/abs/1207.1516"}],"oa":1,"publisher":"American Physical Society"},{"date_updated":"2021-01-12T08:21:56Z","ddc":["570"],"extern":"1","file_date_updated":"2020-07-14T12:48:15Z","_id":"923","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"type":"journal_article","status":"public","publication_status":"published","language":[{"iso":"eng"}],"file":[{"date_created":"2019-05-10T11:20:26Z","file_name":"2011_PLOS1_Fre.PDF","creator":"dernst","date_updated":"2020-07-14T12:48:15Z","file_size":2860615,"checksum":"b4e864125dfcb9fa57a9e01688838081","file_id":"6401","access_level":"open_access","relation":"main_file","content_type":"application/pdf"}],"issue":"10","volume":6,"abstract":[{"text":"The conserved role of Notch signaling in controlling intestinal cell fate specification and homeostasis has been extensively studied. Nevertheless, the precise identity of the cells in which Notch signaling is active and the role of different Notch receptor paralogues in the intestine remain ambiguous, due to the lack of reliable tools to investigate Notch expression and function in vivo. We generated a new series of transgenic mice that allowed us, by lineage analysis, to formally prove that Notch1 and Notch2 are specifically expressed in crypt stem cells. In addition, a novel Notch reporter mouse, Hes1-EmGFP SAT, demonstrated exclusive Notch activity in crypt stem cells and absorptive progenitors. This roster of knock-in and reporter mice represents a valuable resource to functionally explore the Notch pathway in vivo in virtually all tissues.","lang":"eng"}],"oa_version":"Published Version","intvolume":" 6","month":"10","citation":{"ama":"Fré S, Hannezo EB, Šale S, et al. Notch lineages and activity in intestinal stem cells determined by a new set of knock in mice. PLoS One. 2011;6(10). doi:10.1371/journal.pone.0025785","apa":"Fré, S., Hannezo, E. B., Šale, S., Huyghe, M., Lafkas, D., Kissel, H., … Artavanis Tsakonas, S. (2011). Notch lineages and activity in intestinal stem cells determined by a new set of knock in mice. PLoS One. Public Library of Science. https://doi.org/10.1371/journal.pone.0025785","short":"S. Fré, E.B. Hannezo, S. Šale, M. Huyghe, D. Lafkas, H. Kissel, A. Louvi, J. Greve, D. Louvard, S. Artavanis Tsakonas, PLoS One 6 (2011).","ieee":"S. Fré et al., “Notch lineages and activity in intestinal stem cells determined by a new set of knock in mice,” PLoS One, vol. 6, no. 10. Public Library of Science, 2011.","mla":"Fré, Silvia, et al. “Notch Lineages and Activity in Intestinal Stem Cells Determined by a New Set of Knock in Mice.” PLoS One, vol. 6, no. 10, e25785, Public Library of Science, 2011, doi:10.1371/journal.pone.0025785.","ista":"Fré S, Hannezo EB, Šale S, Huyghe M, Lafkas D, Kissel H, Louvi A, Greve J, Louvard D, Artavanis Tsakonas S. 2011. Notch lineages and activity in intestinal stem cells determined by a new set of knock in mice. PLoS One. 6(10), e25785.","chicago":"Fré, Silvia, Edouard B Hannezo, Sanja Šale, Mathilde Huyghe, Daniel Lafkas, Holger Kissel, Angeliki Louvi, Jeffrey Greve, Daniel Louvard, and Spyros Artavanis Tsakonas. “Notch Lineages and Activity in Intestinal Stem Cells Determined by a New Set of Knock in Mice.” PLoS One. Public Library of Science, 2011. https://doi.org/10.1371/journal.pone.0025785."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publist_id":"6520","author":[{"last_name":"Fré","full_name":"Fré, Silvia","first_name":"Silvia"},{"first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561"},{"last_name":"Šale","full_name":"Šale, Sanja","first_name":"Sanja"},{"last_name":"Huyghe","full_name":"Huyghe, Mathilde","first_name":"Mathilde"},{"last_name":"Lafkas","full_name":"Lafkas, Daniel","first_name":"Daniel"},{"first_name":"Holger","last_name":"Kissel","full_name":"Kissel, Holger"},{"last_name":"Louvi","full_name":"Louvi, Angeliki","first_name":"Angeliki"},{"full_name":"Greve, Jeffrey","last_name":"Greve","first_name":"Jeffrey"},{"first_name":"Daniel","full_name":"Louvard, Daniel","last_name":"Louvard"},{"full_name":"Artavanis Tsakonas, Spyros","last_name":"Artavanis Tsakonas","first_name":"Spyros"}],"title":"Notch lineages and activity in intestinal stem cells determined by a new set of knock in mice","article_number":"e25785","year":"2011","has_accepted_license":"1","publication":"PLoS One","day":"03","date_created":"2018-12-11T11:49:13Z","doi":"10.1371/journal.pone.0025785","date_published":"2011-10-03T00:00:00Z","oa":1,"quality_controlled":"1","publisher":"Public Library of Science"},{"title":"Glass-like dynamics of collective cell migration","publist_id":"6522","author":[{"first_name":"Thomas","full_name":"Angelini, Thomas","last_name":"Angelini"},{"first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo"},{"first_name":"Xavier","last_name":"Trepatc","full_name":"Trepatc, Xavier"},{"first_name":"Manuel","full_name":"Marquez, Manuel","last_name":"Marquez"},{"first_name":"Jeffrey","full_name":"Fredberg, Jeffrey","last_name":"Fredberg"},{"last_name":"Weitz","full_name":"Weitz, David","first_name":"David"}],"extern":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2021-01-12T08:21:54Z","citation":{"mla":"Angelini, Thomas, et al. “Glass-like Dynamics of Collective Cell Migration.” Proceedings of the National Academy of Sciences of the United States of America, vol. 108, no. 12, PNAS, 2011, pp. 4714–19, doi:10.1073/pnas.1010059108.","short":"T. Angelini, E.B. Hannezo, X. Trepatc, M. Marquez, J. Fredberg, D. Weitz, Proceedings of the National Academy of Sciences of the United States of America 108 (2011) 4714–4719.","ieee":"T. Angelini, E. B. Hannezo, X. Trepatc, M. Marquez, J. Fredberg, and D. Weitz, “Glass-like dynamics of collective cell migration,” Proceedings of the National Academy of Sciences of the United States of America, vol. 108, no. 12. PNAS, pp. 4714–4719, 2011.","ama":"Angelini T, Hannezo EB, Trepatc X, Marquez M, Fredberg J, Weitz D. Glass-like dynamics of collective cell migration. Proceedings of the National Academy of Sciences of the United States of America. 2011;108(12):4714-4719. doi:10.1073/pnas.1010059108","apa":"Angelini, T., Hannezo, E. B., Trepatc, X., Marquez, M., Fredberg, J., & Weitz, D. (2011). Glass-like dynamics of collective cell migration. Proceedings of the National Academy of Sciences of the United States of America. PNAS. https://doi.org/10.1073/pnas.1010059108","chicago":"Angelini, Thomas, Edouard B Hannezo, Xavier Trepatc, Manuel Marquez, Jeffrey Fredberg, and David Weitz. “Glass-like Dynamics of Collective Cell Migration.” Proceedings of the National Academy of Sciences of the United States of America. PNAS, 2011. https://doi.org/10.1073/pnas.1010059108.","ista":"Angelini T, Hannezo EB, Trepatc X, Marquez M, Fredberg J, Weitz D. 2011. Glass-like dynamics of collective cell migration. Proceedings of the National Academy of Sciences of the United States of America. 108(12), 4714–4719."},"status":"public","type":"journal_article","_id":"919","doi":"10.1073/pnas.1010059108","issue":"12","date_published":"2011-03-22T00:00:00Z","volume":108,"date_created":"2018-12-11T11:49:12Z","page":"4714 - 4719","day":"22","publication":"Proceedings of the National Academy of Sciences of the United States of America","language":[{"iso":"eng"}],"year":"2011","publication_status":"published","month":"03","intvolume":" 108","quality_controlled":"1","publisher":"PNAS","oa_version":"None","abstract":[{"text":"Collective cell migration in tissues occurs throughout embryonic development, during wound healing, and in cancerous tumor invasion, yet most detailed knowledge of cell migration comes from single-cell studies. As single cells migrate, the shape of the cell body fluctuates dramatically through cyclic processes of extension, adhesion, and retraction, accompanied by erratic changes in migration direction. Within confluent cell layers, such subcellular motions must be coupled between neighbors, yet the influence of these subcellular motions on collective migration is not known. Here we study motion within a confluent epithelial cell sheet, simultaneously measuring collective migration and subcellular motions, covering a broad range of length scales, time scales, and cell densities. At large length scales and time scales collective migration slows as cell density rises, yet the fastest cells move in large, multicell groups whose scale grows with increasing cell density. This behavior has an intriguing analogy to dynamic heterogeneities found in particulate systems as they become more crowded and approach a glass transition. In addition we find a diminishing self-diffusivity of short-wavelength motions within the cell layer, and growing peaks in the vibrational density of states associated with cooperative cell-shape fluctuations. Both of these observations are also intriguingly reminiscent of a glass transition. Thus, these results provide a broad and suggestive analogy between cell motion within a confluent layer and the dynamics of supercooled colloidal and molecular fluids approaching a glass transition.","lang":"eng"}]},{"intvolume":" 107","month":"08","publisher":"American Physical Society","acknowledgement":"We thank S. Fre and M. Huygue for discussion and for showing us in vivo samples and A. Bergès for help with the manuscript.","oa_version":"None","abstract":[{"lang":"eng","text":"We study theoretically the shapes of a dividing epithelial monolayer of cells lying on top of an elastic stroma. The negative tension created by cell division provokes a buckling instability at a finite wave vector leading to the formation of periodic arrays of villi and crypts. The instability is similar to the buckling of a metallic plate under compression. We use the results to rationalize the various structures of the intestinal lining observed in vivo. Taking into account the coupling between cell division and local curvature, we obtain different patterns of villi and crypts, which could explain the different morphologies of the small intestine and the colon."}],"date_created":"2018-12-11T11:49:11Z","date_published":"2011-08-11T00:00:00Z","doi":"10.1103/PhysRevLett.107.078104","issue":"7","volume":107,"publication":"Physical Review Letters","language":[{"iso":"eng"}],"day":"11","publication_status":"published","year":"2011","status":"public","type":"journal_article","_id":"918","title":"Instabilities of monolayered epithelia Shape and structure of villi and crypts","article_processing_charge":"No","author":[{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo"},{"last_name":"Prost","full_name":"Prost, Jacques","first_name":"Jacques"},{"full_name":"Joanny, Jean","last_name":"Joanny","first_name":"Jean"}],"publist_id":"6521","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","extern":"1","date_updated":"2021-01-12T08:21:54Z","citation":{"ama":"Hannezo EB, Prost J, Joanny J. Instabilities of monolayered epithelia Shape and structure of villi and crypts. Physical Review Letters. 2011;107(7). doi:10.1103/PhysRevLett.107.078104","apa":"Hannezo, E. B., Prost, J., & Joanny, J. (2011). Instabilities of monolayered epithelia Shape and structure of villi and crypts. Physical Review Letters. American Physical Society. https://doi.org/10.1103/PhysRevLett.107.078104","short":"E.B. Hannezo, J. Prost, J. Joanny, Physical Review Letters 107 (2011).","ieee":"E. B. Hannezo, J. Prost, and J. Joanny, “Instabilities of monolayered epithelia Shape and structure of villi and crypts,” Physical Review Letters, vol. 107, no. 7. American Physical Society, 2011.","mla":"Hannezo, Edouard B., et al. “Instabilities of Monolayered Epithelia Shape and Structure of Villi and Crypts.” Physical Review Letters, vol. 107, no. 7, American Physical Society, 2011, doi:10.1103/PhysRevLett.107.078104.","ista":"Hannezo EB, Prost J, Joanny J. 2011. Instabilities of monolayered epithelia Shape and structure of villi and crypts. Physical Review Letters. 107(7).","chicago":"Hannezo, Edouard B, Jacques Prost, and Jean Joanny. “Instabilities of Monolayered Epithelia Shape and Structure of Villi and Crypts.” Physical Review Letters. American Physical Society, 2011. https://doi.org/10.1103/PhysRevLett.107.078104."}},{"status":"public","type":"journal_article","_id":"920","title":"Cell migration driven by cooperative substrate deformation patterns","author":[{"first_name":"Thomas","last_name":"Angelini","full_name":"Angelini, Thomas"},{"last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Xavier","full_name":"Trepat, Xavier","last_name":"Trepat"},{"last_name":"Fredberg","full_name":"Fredberg, Jeffrey","first_name":"Jeffrey"},{"full_name":"Weitz, David","last_name":"Weitz","first_name":"David"}],"publist_id":"6523","article_processing_charge":"No","extern":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2021-01-12T08:21:55Z","citation":{"mla":"Angelini, Thomas, et al. “Cell Migration Driven by Cooperative Substrate Deformation Patterns.” Physical Review Letters, vol. 104, no. 16, American Physical Society, 2010, doi:10.1103/PhysRevLett.104.168104.","short":"T. Angelini, E.B. Hannezo, X. Trepat, J. Fredberg, D. Weitz, Physical Review Letters 104 (2010).","ieee":"T. Angelini, E. B. Hannezo, X. Trepat, J. Fredberg, and D. Weitz, “Cell migration driven by cooperative substrate deformation patterns,” Physical Review Letters, vol. 104, no. 16. American Physical Society, 2010.","apa":"Angelini, T., Hannezo, E. B., Trepat, X., Fredberg, J., & Weitz, D. (2010). Cell migration driven by cooperative substrate deformation patterns. Physical Review Letters. American Physical Society. https://doi.org/10.1103/PhysRevLett.104.168104","ama":"Angelini T, Hannezo EB, Trepat X, Fredberg J, Weitz D. Cell migration driven by cooperative substrate deformation patterns. Physical Review Letters. 2010;104(16). doi:10.1103/PhysRevLett.104.168104","chicago":"Angelini, Thomas, Edouard B Hannezo, Xavier Trepat, Jeffrey Fredberg, and David Weitz. “Cell Migration Driven by Cooperative Substrate Deformation Patterns.” Physical Review Letters. American Physical Society, 2010. https://doi.org/10.1103/PhysRevLett.104.168104.","ista":"Angelini T, Hannezo EB, Trepat X, Fredberg J, Weitz D. 2010. Cell migration driven by cooperative substrate deformation patterns. Physical Review Letters. 104(16)."},"month":"04","intvolume":" 104","publisher":"American Physical Society","oa_version":"None","acknowledgement":"This work was supported by the NSF (DMR-0602684) and the Harvard MRSEC (DMR-0820484).\r\nWe would like to thank Dr. James Butler for helpful conversations.","abstract":[{"text":"Most eukaryotic cells sense and respond to the mechanical properties of their surroundings. This can strongly influence their collective behavior in embryonic development, tissue function, and wound healing. We use a deformable substrate to measure collective behavior in cell motion due to substrate mediated cell-cell interactions. We quantify spatial and temporal correlations in migration velocity and substrate deformation, and show that cooperative cell-driven patterns of substrate deformation mediate long-distance mechanical coupling between cells and control collective cell migration.","lang":"eng"}],"date_published":"2010-04-23T00:00:00Z","volume":104,"issue":"16","doi":"10.1103/PhysRevLett.104.168104","date_created":"2018-12-11T11:49:12Z","day":"23","language":[{"iso":"eng"}],"publication":"Physical Review Letters","publication_status":"published","year":"2010"}]