--- _id: '1284' abstract: - lang: eng text: Brassinosteroids (BRs) are growth-promoting plant hormones that play a role in abiotic stress responses, but molecular modes that enable this activity remain largely unknown. Here we show that BRs participate in the regulation of freezing tolerance. BR signaling-defective mutants of Arabidopsis thaliana were hypersensitive to freezing before and after cold acclimation. The constitutive activation of BR signaling, in contrast, enhanced freezing resistance. Evidence is provided that the BR-controlled basic helix-loop-helix transcription factor CESTA (CES) can contribute to the constitutive expression of the C-REPEAT/DEHYDRATION-RESPONSIVE ELEMENT BINDING FACTOR (CBF) transcriptional regulators that control cold responsive (COR) gene expression. In addition, CBF-independent classes of BR-regulated COR genes are identified that are regulated in a BR- and CES-dependent manner during cold acclimation. A model is presented in which BRs govern different cold-responsive transcriptional cascades through the post-translational modification of CES and redundantly acting factors. This contributes to the basal resistance against freezing stress, but also to the further improvement of this resistance through cold acclimation. acknowledgement: 'We thank Joanne Chory for seeds of the bee1 bee2 bee3, bes1-D, and bzr1-1D mutants and the 35S:BRI1-GFP line; Irene Ziegler, Clarissa Fahrig, and Renata Milcevicova for technical assistance; and the horticultural staff of the TUMs Gewächshauslaborzentrum Dürnast for plant care. This work was supported by funds from the Austrian Science Fund (Project P22734 to B.P.), the Deutsche Forschungsgemeinschaft (Project PO1640/4 to B.P. and SFB924 to B.P. and K.F.X.M.), and a TUM doctoral fellowship (to M.E.). M.E. and S.J.U. were members of the TUM graduate school. ' article_processing_charge: No author: - first_name: Marina full_name: Eremina, Marina last_name: Eremina - first_name: Simon full_name: Unterholzner, Simon last_name: Unterholzner - first_name: Ajith full_name: Rathnayake, Ajith last_name: Rathnayake - first_name: Marcos full_name: Castellanos, Marcos last_name: Castellanos - first_name: Mamoona full_name: Khan-Djamei, Mamoona id: 391B5BBC-F248-11E8-B48F-1D18A9856A87 last_name: Khan-Djamei - first_name: Karl full_name: Kügler, Karl last_name: Kügler - first_name: Sean full_name: May, Sean last_name: May - first_name: Klaus full_name: Mayer, Klaus last_name: Mayer - first_name: Wilfried full_name: Rozhon, Wilfried last_name: Rozhon - first_name: Brigitte full_name: Poppenberger, Brigitte last_name: Poppenberger citation: ama: Eremina M, Unterholzner S, Rathnayake A, et al. Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants. PNAS. 2016;113(40):E5982-E5991. doi:10.1073/pnas.1611477113 apa: Eremina, M., Unterholzner, S., Rathnayake, A., Castellanos, M., Khan-Djamei, M., Kügler, K., … Poppenberger, B. (2016). Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1611477113 chicago: Eremina, Marina, Simon Unterholzner, Ajith Rathnayake, Marcos Castellanos, Mamoona Khan-Djamei, Karl Kügler, Sean May, Klaus Mayer, Wilfried Rozhon, and Brigitte Poppenberger. “Brassinosteroids Participate in the Control of Basal and Acquired Freezing Tolerance of Plants.” PNAS. National Academy of Sciences, 2016. https://doi.org/10.1073/pnas.1611477113. ieee: M. Eremina et al., “Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants,” PNAS, vol. 113, no. 40. National Academy of Sciences, pp. E5982–E5991, 2016. ista: Eremina M, Unterholzner S, Rathnayake A, Castellanos M, Khan-Djamei M, Kügler K, May S, Mayer K, Rozhon W, Poppenberger B. 2016. Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants. PNAS. 113(40), E5982–E5991. mla: Eremina, Marina, et al. “Brassinosteroids Participate in the Control of Basal and Acquired Freezing Tolerance of Plants.” PNAS, vol. 113, no. 40, National Academy of Sciences, 2016, pp. E5982–91, doi:10.1073/pnas.1611477113. short: M. Eremina, S. Unterholzner, A. Rathnayake, M. Castellanos, M. Khan-Djamei, K. Kügler, S. May, K. Mayer, W. Rozhon, B. Poppenberger, PNAS 113 (2016) E5982–E5991. date_created: 2018-12-11T11:51:08Z date_published: 2016-10-04T00:00:00Z date_updated: 2022-02-18T13:41:37Z day: '04' doi: 10.1073/pnas.1611477113 extern: '1' external_id: pmid: - '27489342' intvolume: ' 113' issue: '40' language: - iso: eng main_file_link: - open_access: '1' url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5056081/ month: '10' oa: 1 oa_version: Submitted Version page: E5982 - E5991 pmid: 1 publication: PNAS publication_status: published publisher: National Academy of Sciences publist_id: '6032' quality_controlled: '1' scopus_import: '1' status: public title: Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 113 year: '2016' ... --- _id: '1640' abstract: - lang: eng text: Auxin and cytokinin are key endogenous regulators of plant development. Although cytokinin-mediated modulation of auxin distribution is a developmentally crucial hormonal interaction, its molecular basis is largely unknown. Here we show a direct regulatory link between cytokinin signalling and the auxin transport machinery uncovering a mechanistic framework for cytokinin-auxin cross-talk. We show that the CYTOKININ RESPONSE FACTORS (CRFs), transcription factors downstream of cytokinin perception, transcriptionally control genes encoding PIN-FORMED (PIN) auxin transporters at a specific PIN CYTOKININ RESPONSE ELEMENT (PCRE) domain. Removal of this cis-regulatory element effectively uncouples PIN transcription from the CRF-mediated cytokinin regulation and attenuates plant cytokinin sensitivity. We propose that CRFs represent a missing cross-talk component that fine-tunes auxin transport capacity downstream of cytokinin signalling to control plant development. acknowledged_ssus: - _id: Bio - _id: LifeSc acknowledgement: This work was supported by the European Research Council Starting Independent Research grant (ERC-2007-Stg-207362-HCPO to E.B., M.S., C.C.), by the Ghent University Multidisciplinary Research Partnership ‘Biotechnology for a Sustainable Economy’ no.01MRB510W, by the Research Foundation—Flanders (grant 3G033711 to J.-A.O.), by the Austrian Science Fund (FWF01_I1774S) to K.Ö.,E.B., and by the Interuniversity Attraction Poles Programme (IUAP P7/29 ‘MARS’) initiated by the Belgian Science Policy Office. I.D.C. and S.V. are post-doctoral fellows of the Research Foundation—Flanders (FWO). This research was supported by the Scientific Service Units (SSU) of IST-Austria through resources provided by the Bioimaging Facility (BIF), the Life Science Facility (LSF). article_number: '8717' author: - first_name: Mária full_name: Šimášková, Mária last_name: Šimášková - first_name: José full_name: O'Brien, José last_name: O'Brien - first_name: Mamoona full_name: Khan-Djamei, Mamoona id: 391B5BBC-F248-11E8-B48F-1D18A9856A87 last_name: Khan-Djamei - first_name: Giel full_name: Van Noorden, Giel last_name: Van Noorden - first_name: Krisztina full_name: Ötvös, Krisztina id: 29B901B0-F248-11E8-B48F-1D18A9856A87 last_name: Ötvös orcid: 0000-0002-5503-4983 - first_name: Anne full_name: Vieten, Anne last_name: Vieten - first_name: Inge full_name: De Clercq, Inge last_name: De Clercq - first_name: Johanna full_name: Van Haperen, Johanna last_name: Van Haperen - first_name: Candela full_name: Cuesta, Candela id: 33A3C818-F248-11E8-B48F-1D18A9856A87 last_name: Cuesta orcid: 0000-0003-1923-2410 - first_name: Klára full_name: Hoyerová, Klára last_name: Hoyerová - first_name: Steffen full_name: Vanneste, Steffen last_name: Vanneste - first_name: Peter full_name: Marhavy, Peter id: 3F45B078-F248-11E8-B48F-1D18A9856A87 last_name: Marhavy orcid: 0000-0001-5227-5741 - first_name: Krzysztof T full_name: Wabnik, Krzysztof T id: 4DE369A4-F248-11E8-B48F-1D18A9856A87 last_name: Wabnik orcid: 0000-0001-7263-0560 - first_name: Frank full_name: Van Breusegem, Frank last_name: Van Breusegem - first_name: Moritz full_name: Nowack, Moritz last_name: Nowack - first_name: Angus full_name: Murphy, Angus last_name: Murphy - first_name: Jiřĺ full_name: Friml, Jiřĺ id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 - first_name: Dolf full_name: Weijers, Dolf last_name: Weijers - first_name: Tom full_name: Beeckman, Tom last_name: Beeckman - first_name: Eva full_name: Benková, Eva id: 38F4F166-F248-11E8-B48F-1D18A9856A87 last_name: Benková orcid: 0000-0002-8510-9739 citation: ama: Šimášková M, O’Brien J, Khan-Djamei M, et al. Cytokinin response factors regulate PIN-FORMED auxin transporters. Nature Communications. 2015;6. doi:10.1038/ncomms9717 apa: Šimášková, M., O’Brien, J., Khan-Djamei, M., Van Noorden, G., Ötvös, K., Vieten, A., … Benková, E. (2015). Cytokinin response factors regulate PIN-FORMED auxin transporters. Nature Communications. Nature Publishing Group. https://doi.org/10.1038/ncomms9717 chicago: Šimášková, Mária, José O’Brien, Mamoona Khan-Djamei, Giel Van Noorden, Krisztina Ötvös, Anne Vieten, Inge De Clercq, et al. “Cytokinin Response Factors Regulate PIN-FORMED Auxin Transporters.” Nature Communications. Nature Publishing Group, 2015. https://doi.org/10.1038/ncomms9717. ieee: M. Šimášková et al., “Cytokinin response factors regulate PIN-FORMED auxin transporters,” Nature Communications, vol. 6. Nature Publishing Group, 2015. ista: Šimášková M, O’Brien J, Khan-Djamei M, Van Noorden G, Ötvös K, Vieten A, De Clercq I, Van Haperen J, Cuesta C, Hoyerová K, Vanneste S, Marhavý P, Wabnik KT, Van Breusegem F, Nowack M, Murphy A, Friml J, Weijers D, Beeckman T, Benková E. 2015. Cytokinin response factors regulate PIN-FORMED auxin transporters. Nature Communications. 6, 8717. mla: Šimášková, Mária, et al. “Cytokinin Response Factors Regulate PIN-FORMED Auxin Transporters.” Nature Communications, vol. 6, 8717, Nature Publishing Group, 2015, doi:10.1038/ncomms9717. short: M. Šimášková, J. O’Brien, M. Khan-Djamei, G. Van Noorden, K. Ötvös, A. Vieten, I. De Clercq, J. Van Haperen, C. Cuesta, K. Hoyerová, S. Vanneste, P. Marhavý, K.T. Wabnik, F. Van Breusegem, M. Nowack, A. Murphy, J. Friml, D. Weijers, T. Beeckman, E. Benková, Nature Communications 6 (2015). date_created: 2018-12-11T11:53:12Z date_published: 2015-01-01T00:00:00Z date_updated: 2021-01-12T06:52:11Z day: '01' ddc: - '580' department: - _id: EvBe - _id: JiFr doi: 10.1038/ncomms9717 ec_funded: 1 file: - access_level: open_access checksum: c2c84bca37401435fedf76bad0ba0579 content_type: application/pdf creator: system date_created: 2018-12-12T10:18:36Z date_updated: 2020-07-14T12:45:08Z file_id: '5358' file_name: IST-2018-1020-v1+1_Simaskova_et_al_NatCom_2015.pdf file_size: 1471217 relation: main_file file_date_updated: 2020-07-14T12:45:08Z has_accepted_license: '1' intvolume: ' 6' language: - iso: eng month: '01' oa: 1 oa_version: Submitted Version project: - _id: 253FCA6A-B435-11E9-9278-68D0E5697425 call_identifier: FP7 grant_number: '207362' name: Hormonal cross-talk in plant organogenesis - _id: 2542D156-B435-11E9-9278-68D0E5697425 call_identifier: FWF grant_number: I 1774-B16 name: Hormone cross-talk drives nutrient dependent plant development publication: Nature Communications publication_status: published publisher: Nature Publishing Group publist_id: '5513' pubrep_id: '1020' quality_controlled: '1' scopus_import: 1 status: public title: Cytokinin response factors regulate PIN-FORMED auxin transporters type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 6 year: '2015' ...