---
_id: '1284'
abstract:
- lang: eng
text: Brassinosteroids (BRs) are growth-promoting plant hormones that play a role
in abiotic stress responses, but molecular modes that enable this activity remain
largely unknown. Here we show that BRs participate in the regulation of freezing
tolerance. BR signaling-defective mutants of Arabidopsis thaliana were hypersensitive
to freezing before and after cold acclimation. The constitutive activation of
BR signaling, in contrast, enhanced freezing resistance. Evidence is provided
that the BR-controlled basic helix-loop-helix transcription factor CESTA (CES)
can contribute to the constitutive expression of the C-REPEAT/DEHYDRATION-RESPONSIVE
ELEMENT BINDING FACTOR (CBF) transcriptional regulators that control cold responsive
(COR) gene expression. In addition, CBF-independent classes of BR-regulated COR
genes are identified that are regulated in a BR- and CES-dependent manner during
cold acclimation. A model is presented in which BRs govern different cold-responsive
transcriptional cascades through the post-translational modification of CES and
redundantly acting factors. This contributes to the basal resistance against freezing
stress, but also to the further improvement of this resistance through cold acclimation.
acknowledgement: 'We thank Joanne Chory for seeds of the bee1 bee2 bee3, bes1-D, and
bzr1-1D mutants and the 35S:BRI1-GFP line; Irene Ziegler, Clarissa Fahrig, and Renata
Milcevicova for technical assistance; and the horticultural staff of the TUMs Gewächshauslaborzentrum
Dürnast for plant care. This work was supported by funds from the Austrian Science
Fund (Project P22734 to B.P.), the Deutsche Forschungsgemeinschaft (Project PO1640/4
to B.P. and SFB924 to B.P. and K.F.X.M.), and a TUM doctoral fellowship (to M.E.).
M.E. and S.J.U. were members of the TUM graduate school. '
article_processing_charge: No
author:
- first_name: Marina
full_name: Eremina, Marina
last_name: Eremina
- first_name: Simon
full_name: Unterholzner, Simon
last_name: Unterholzner
- first_name: Ajith
full_name: Rathnayake, Ajith
last_name: Rathnayake
- first_name: Marcos
full_name: Castellanos, Marcos
last_name: Castellanos
- first_name: Mamoona
full_name: Khan-Djamei, Mamoona
id: 391B5BBC-F248-11E8-B48F-1D18A9856A87
last_name: Khan-Djamei
- first_name: Karl
full_name: Kügler, Karl
last_name: Kügler
- first_name: Sean
full_name: May, Sean
last_name: May
- first_name: Klaus
full_name: Mayer, Klaus
last_name: Mayer
- first_name: Wilfried
full_name: Rozhon, Wilfried
last_name: Rozhon
- first_name: Brigitte
full_name: Poppenberger, Brigitte
last_name: Poppenberger
citation:
ama: Eremina M, Unterholzner S, Rathnayake A, et al. Brassinosteroids participate
in the control of basal and acquired freezing tolerance of plants. PNAS.
2016;113(40):E5982-E5991. doi:10.1073/pnas.1611477113
apa: Eremina, M., Unterholzner, S., Rathnayake, A., Castellanos, M., Khan-Djamei,
M., Kügler, K., … Poppenberger, B. (2016). Brassinosteroids participate in the
control of basal and acquired freezing tolerance of plants. PNAS. National
Academy of Sciences. https://doi.org/10.1073/pnas.1611477113
chicago: Eremina, Marina, Simon Unterholzner, Ajith Rathnayake, Marcos Castellanos,
Mamoona Khan-Djamei, Karl Kügler, Sean May, Klaus Mayer, Wilfried Rozhon, and
Brigitte Poppenberger. “Brassinosteroids Participate in the Control of Basal and
Acquired Freezing Tolerance of Plants.” PNAS. National Academy of Sciences,
2016. https://doi.org/10.1073/pnas.1611477113.
ieee: M. Eremina et al., “Brassinosteroids participate in the control of
basal and acquired freezing tolerance of plants,” PNAS, vol. 113, no. 40.
National Academy of Sciences, pp. E5982–E5991, 2016.
ista: Eremina M, Unterholzner S, Rathnayake A, Castellanos M, Khan-Djamei M, Kügler
K, May S, Mayer K, Rozhon W, Poppenberger B. 2016. Brassinosteroids participate
in the control of basal and acquired freezing tolerance of plants. PNAS. 113(40),
E5982–E5991.
mla: Eremina, Marina, et al. “Brassinosteroids Participate in the Control of Basal
and Acquired Freezing Tolerance of Plants.” PNAS, vol. 113, no. 40, National
Academy of Sciences, 2016, pp. E5982–91, doi:10.1073/pnas.1611477113.
short: M. Eremina, S. Unterholzner, A. Rathnayake, M. Castellanos, M. Khan-Djamei,
K. Kügler, S. May, K. Mayer, W. Rozhon, B. Poppenberger, PNAS 113 (2016) E5982–E5991.
date_created: 2018-12-11T11:51:08Z
date_published: 2016-10-04T00:00:00Z
date_updated: 2022-02-18T13:41:37Z
day: '04'
doi: 10.1073/pnas.1611477113
extern: '1'
external_id:
pmid:
- '27489342'
intvolume: ' 113'
issue: '40'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5056081/
month: '10'
oa: 1
oa_version: Submitted Version
page: E5982 - E5991
pmid: 1
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '6032'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Brassinosteroids participate in the control of basal and acquired freezing
tolerance of plants
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 113
year: '2016'
...
---
_id: '1640'
abstract:
- lang: eng
text: Auxin and cytokinin are key endogenous regulators of plant development. Although
cytokinin-mediated modulation of auxin distribution is a developmentally crucial
hormonal interaction, its molecular basis is largely unknown. Here we show a direct
regulatory link between cytokinin signalling and the auxin transport machinery
uncovering a mechanistic framework for cytokinin-auxin cross-talk. We show that
the CYTOKININ RESPONSE FACTORS (CRFs), transcription factors downstream of cytokinin
perception, transcriptionally control genes encoding PIN-FORMED (PIN) auxin transporters
at a specific PIN CYTOKININ RESPONSE ELEMENT (PCRE) domain. Removal of this cis-regulatory
element effectively uncouples PIN transcription from the CRF-mediated cytokinin
regulation and attenuates plant cytokinin sensitivity. We propose that CRFs represent
a missing cross-talk component that fine-tunes auxin transport capacity downstream
of cytokinin signalling to control plant development.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: This work was supported by the European Research Council Starting
Independent Research grant (ERC-2007-Stg-207362-HCPO to E.B., M.S., C.C.), by the
Ghent University Multidisciplinary Research Partnership ‘Biotechnology for a Sustainable
Economy’ no.01MRB510W, by the Research Foundation—Flanders (grant 3G033711 to J.-A.O.),
by the Austrian Science Fund (FWF01_I1774S) to K.Ö.,E.B., and by the Interuniversity
Attraction Poles Programme (IUAP P7/29 ‘MARS’) initiated by the Belgian Science
Policy Office. I.D.C. and S.V. are post-doctoral fellows of the Research Foundation—Flanders
(FWO). This research was supported by the Scientific Service Units (SSU) of IST-Austria
through resources provided by the Bioimaging Facility (BIF), the Life Science Facility
(LSF).
article_number: '8717'
author:
- first_name: Mária
full_name: Šimášková, Mária
last_name: Šimášková
- first_name: José
full_name: O'Brien, José
last_name: O'Brien
- first_name: Mamoona
full_name: Khan-Djamei, Mamoona
id: 391B5BBC-F248-11E8-B48F-1D18A9856A87
last_name: Khan-Djamei
- first_name: Giel
full_name: Van Noorden, Giel
last_name: Van Noorden
- first_name: Krisztina
full_name: Ötvös, Krisztina
id: 29B901B0-F248-11E8-B48F-1D18A9856A87
last_name: Ötvös
orcid: 0000-0002-5503-4983
- first_name: Anne
full_name: Vieten, Anne
last_name: Vieten
- first_name: Inge
full_name: De Clercq, Inge
last_name: De Clercq
- first_name: Johanna
full_name: Van Haperen, Johanna
last_name: Van Haperen
- first_name: Candela
full_name: Cuesta, Candela
id: 33A3C818-F248-11E8-B48F-1D18A9856A87
last_name: Cuesta
orcid: 0000-0003-1923-2410
- first_name: Klára
full_name: Hoyerová, Klára
last_name: Hoyerová
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Peter
full_name: Marhavy, Peter
id: 3F45B078-F248-11E8-B48F-1D18A9856A87
last_name: Marhavy
orcid: 0000-0001-5227-5741
- first_name: Krzysztof T
full_name: Wabnik, Krzysztof T
id: 4DE369A4-F248-11E8-B48F-1D18A9856A87
last_name: Wabnik
orcid: 0000-0001-7263-0560
- first_name: Frank
full_name: Van Breusegem, Frank
last_name: Van Breusegem
- first_name: Moritz
full_name: Nowack, Moritz
last_name: Nowack
- first_name: Angus
full_name: Murphy, Angus
last_name: Murphy
- first_name: Jiřĺ
full_name: Friml, Jiřĺ
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
- first_name: Tom
full_name: Beeckman, Tom
last_name: Beeckman
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Šimášková M, O’Brien J, Khan-Djamei M, et al. Cytokinin response factors regulate
PIN-FORMED auxin transporters. Nature Communications. 2015;6. doi:10.1038/ncomms9717
apa: Šimášková, M., O’Brien, J., Khan-Djamei, M., Van Noorden, G., Ötvös, K., Vieten,
A., … Benková, E. (2015). Cytokinin response factors regulate PIN-FORMED auxin
transporters. Nature Communications. Nature Publishing Group. https://doi.org/10.1038/ncomms9717
chicago: Šimášková, Mária, José O’Brien, Mamoona Khan-Djamei, Giel Van Noorden,
Krisztina Ötvös, Anne Vieten, Inge De Clercq, et al. “Cytokinin Response Factors
Regulate PIN-FORMED Auxin Transporters.” Nature Communications. Nature
Publishing Group, 2015. https://doi.org/10.1038/ncomms9717.
ieee: M. Šimášková et al., “Cytokinin response factors regulate PIN-FORMED
auxin transporters,” Nature Communications, vol. 6. Nature Publishing Group,
2015.
ista: Šimášková M, O’Brien J, Khan-Djamei M, Van Noorden G, Ötvös K, Vieten A, De
Clercq I, Van Haperen J, Cuesta C, Hoyerová K, Vanneste S, Marhavý P, Wabnik KT,
Van Breusegem F, Nowack M, Murphy A, Friml J, Weijers D, Beeckman T, Benková E.
2015. Cytokinin response factors regulate PIN-FORMED auxin transporters. Nature
Communications. 6, 8717.
mla: Šimášková, Mária, et al. “Cytokinin Response Factors Regulate PIN-FORMED Auxin
Transporters.” Nature Communications, vol. 6, 8717, Nature Publishing Group,
2015, doi:10.1038/ncomms9717.
short: M. Šimášková, J. O’Brien, M. Khan-Djamei, G. Van Noorden, K. Ötvös, A. Vieten,
I. De Clercq, J. Van Haperen, C. Cuesta, K. Hoyerová, S. Vanneste, P. Marhavý,
K.T. Wabnik, F. Van Breusegem, M. Nowack, A. Murphy, J. Friml, D. Weijers, T.
Beeckman, E. Benková, Nature Communications 6 (2015).
date_created: 2018-12-11T11:53:12Z
date_published: 2015-01-01T00:00:00Z
date_updated: 2021-01-12T06:52:11Z
day: '01'
ddc:
- '580'
department:
- _id: EvBe
- _id: JiFr
doi: 10.1038/ncomms9717
ec_funded: 1
file:
- access_level: open_access
checksum: c2c84bca37401435fedf76bad0ba0579
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:18:36Z
date_updated: 2020-07-14T12:45:08Z
file_id: '5358'
file_name: IST-2018-1020-v1+1_Simaskova_et_al_NatCom_2015.pdf
file_size: 1471217
relation: main_file
file_date_updated: 2020-07-14T12:45:08Z
has_accepted_license: '1'
intvolume: ' 6'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Submitted Version
project:
- _id: 253FCA6A-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '207362'
name: Hormonal cross-talk in plant organogenesis
- _id: 2542D156-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I 1774-B16
name: Hormone cross-talk drives nutrient dependent plant development
publication: Nature Communications
publication_status: published
publisher: Nature Publishing Group
publist_id: '5513'
pubrep_id: '1020'
quality_controlled: '1'
scopus_import: 1
status: public
title: Cytokinin response factors regulate PIN-FORMED auxin transporters
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 6
year: '2015'
...