---
_id: '3289'
abstract:
- lang: eng
text: "Viral manipulation of transduction pathways associated with key cellular
functions such as survival, response to microbial infection, and cytoskeleton
reorganization can provide the supportive milieu for a productive infection. Here,
we demonstrate that vaccinia virus (VACV) infection leads to activation of the
stress-activated protein kinase (SAPK)/extracellular signal-regulated kinase (ERK)
4/7 (MKK4/7)-c-Jun N-terminal protein kinase 1/2 (JNK1/2) pathway; further, the
stimulation of this pathway requires postpenetration, prereplicative events in
the viral replication cycle. Although the formation of intracellular mature virus
(IMV) was not affected in MKK4/7- or JNK1/2-knockout (KO) cells, we did note an
accentuated deregulation of microtubule and actin network organization in infected
JNK1/2-KO cells. This was followed by deregulated viral trafficking to the periphery
and enhanced enveloped particle release. Furthermore, VACV infection induced alterations
in the cell contractility and morphology, and cell migration was reduced in the
JNK-KO cells. In addition, phosphorylation of proteins implicated with early cell
contractility and cell migration, such as microtubule-associated protein 1B and
paxillin, respectively, was not detected in the VACV-infected KO cells. In sum,
our findings uncover a regulatory role played by the MKK4/7-JNK1/2 pathway in
cytoskeleton reorganization during VACV infection.\r\n"
acknowledgement: "This work was supported by grants from Fundação de Amparo a Pesquisa
do Estado de Minas Gerais (FAPEMIG), the Brazilian Federal Agency for Support and
Evaluation of Graduate Education (CAPES), and the National Council for Scientific
and Technological Development (CNPq). A.C.T.C.P., B.S.A.F.B., F.G.G.L., and J.A.P.S.-M.
were recipients of predoctoral fellowships from CNPq. C.A.B., E.G.K., T.S.-P., P.F.P.P.,
and P.C.P.F. are recipients of research fellowships from CNPq. \r\n\r\n\r\nWe are
grateful to Angela S. Lopes, Ilda M. V. Gama, João R. dos Santos, and Andreza A.
Carvalho for their secretarial/technical assistance and to Fernanda Gambogi for
help with immunofluorescence microscopy. We also thank M. C. Sogayar (Department
of Biochemistry, University of São Paulo, São Paulo, Brazil), who kindly provided
us with the A31 cell line, and R. Davis (Howard Hughes Medical Institute, University
of Massachusetts Medical School, Worcester, MA) for the WT and JNK1/2-, MKK4-, MKK7-,
and MKK4/7-KO cells. VACV WR was from C. Jungwirth (Universität Würzburg, Würzburg,
Germany). The recombinant VACV vF13L-GFP and the rabbit polyclonal antibodies against
viral proteins, B5R, D8L, L1R, and A36R, were from B. Moss (NIAID, Bethesda, MD).
The pcDNA3-Myc-JNK2-MKK7 WT plasmid was from Eugen Kerkhoff (Universität Würzburg,
Würzburg, Germany). We also thank Flávio G. da Fonseca (UFMG, Belo Horizonte, MG,
Brazil) and Kathleen A. Boyle (Medical College of Wisconsin, Milwaukee, WI) for
critically reading the manuscript."
author:
- first_name: Anna
full_name: Pereira, Anna
last_name: Pereira
- first_name: Flávia
full_name: Leite, Flávia
id: 36705F98-F248-11E8-B48F-1D18A9856A87
last_name: Leite
- first_name: Bruno
full_name: Brasil, Bruno
last_name: Brasil
- first_name: Jamaria
full_name: Soares Martins, Jamaria
last_name: Soares Martins
- first_name: Alice
full_name: Torres, Alice
last_name: Torres
- first_name: Paulo
full_name: Pimenta, Paulo
last_name: Pimenta
- first_name: Thais
full_name: Souto Padrón, Thais
last_name: Souto Padrón
- first_name: Paula
full_name: Tranktman, Paula
last_name: Tranktman
- first_name: Paulo
full_name: Ferreira, Paulo
last_name: Ferreira
- first_name: Erna
full_name: Kroon, Erna
last_name: Kroon
- first_name: Cláudio
full_name: Bonjardim, Cláudio
last_name: Bonjardim
citation:
ama: Pereira A, Leite F, Brasil B, et al. A vaccinia virus-driven interplay between
the MKK4/7-JNK1/2 pathway and cytoskeleton reorganization. Journal of Virology.
2012;86(1):172-184. doi:10.1128/JVI.05638-11
apa: Pereira, A., Leite, F., Brasil, B., Soares Martins, J., Torres, A., Pimenta,
P., … Bonjardim, C. (2012). A vaccinia virus-driven interplay between the MKK4/7-JNK1/2
pathway and cytoskeleton reorganization. Journal of Virology. ASM. https://doi.org/10.1128/JVI.05638-11
chicago: Pereira, Anna, Flávia Leite, Bruno Brasil, Jamaria Soares Martins, Alice
Torres, Paulo Pimenta, Thais Souto Padrón, et al. “A Vaccinia Virus-Driven Interplay
between the MKK4/7-JNK1/2 Pathway and Cytoskeleton Reorganization.” Journal
of Virology. ASM, 2012. https://doi.org/10.1128/JVI.05638-11.
ieee: A. Pereira et al., “A vaccinia virus-driven interplay between the MKK4/7-JNK1/2
pathway and cytoskeleton reorganization,” Journal of Virology, vol. 86,
no. 1. ASM, pp. 172–184, 2012.
ista: Pereira A, Leite F, Brasil B, Soares Martins J, Torres A, Pimenta P, Souto
Padrón T, Tranktman P, Ferreira P, Kroon E, Bonjardim C. 2012. A vaccinia virus-driven
interplay between the MKK4/7-JNK1/2 pathway and cytoskeleton reorganization. Journal
of Virology. 86(1), 172–184.
mla: Pereira, Anna, et al. “A Vaccinia Virus-Driven Interplay between the MKK4/7-JNK1/2
Pathway and Cytoskeleton Reorganization.” Journal of Virology, vol. 86,
no. 1, ASM, 2012, pp. 172–84, doi:10.1128/JVI.05638-11.
short: A. Pereira, F. Leite, B. Brasil, J. Soares Martins, A. Torres, P. Pimenta,
T. Souto Padrón, P. Tranktman, P. Ferreira, E. Kroon, C. Bonjardim, Journal of
Virology 86 (2012) 172–184.
date_created: 2018-12-11T12:02:29Z
date_published: 2012-01-01T00:00:00Z
date_updated: 2021-01-12T07:42:25Z
day: '01'
department:
- _id: JoBo
doi: 10.1128/JVI.05638-11
external_id:
pmid:
- '22031940'
intvolume: ' 86'
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3255887/
month: '01'
oa: 1
oa_version: Submitted Version
page: 172 - 184
pmid: 1
publication: Journal of Virology
publication_status: published
publisher: ASM
publist_id: '3356'
quality_controlled: '1'
scopus_import: 1
status: public
title: A vaccinia virus-driven interplay between the MKK4/7-JNK1/2 pathway and cytoskeleton
reorganization
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 86
year: '2012'
...