---
_id: '14251'
abstract:
- lang: eng
text: The phytohormone auxin and its directional transport through tissues play
a fundamental role in development of higher plants. This polar auxin transport
predominantly relies on PIN-FORMED (PIN) auxin exporters. Hence, PIN polarization
is crucial for development, but its evolution during the rise of morphological
complexity in land plants remains unclear. Here, we performed a cross-species
investigation by observing the trafficking and localization of endogenous and
exogenous PINs in two bryophytes, Physcomitrium patens and Marchantia polymorpha,
and in the flowering plant Arabidopsis thaliana. We confirmed that the GFP fusion
did not compromise the auxin export function of all examined PINs by using radioactive
auxin export assay and by observing the phenotypic changes in transgenic bryophytes.
Endogenous PINs polarize to filamentous apices, while exogenous Arabidopsis PINs
distribute symmetrically on the membrane in both bryophytes. In Arabidopsis root
epidermis, bryophytic PINs show no defined polarity. Pharmacological interference
revealed a strong cytoskeleton dependence of bryophytic but not Arabidopsis PIN
polarization. The divergence of PIN polarization and trafficking is also observed
within the bryophyte clade and between tissues of individual species. These results
collectively reveal a divergence of PIN trafficking and polarity mechanisms throughout
land plant evolution and a co-evolution of PIN sequence-based and cell-based polarity
mechanisms.
acknowledgement: This work was supported by the ERC grant (PR1023ERC02) to H. T. and
J. F., and by the ministry of science and technology (grant number 110-2636-B-005-001)
to K. J. L.
article_number: '100669'
article_processing_charge: Yes
article_type: original
author:
- first_name: Han
full_name: Tang, Han
id: 19BDF720-25A0-11EA-AC6E-928F3DDC885E
last_name: Tang
orcid: 0000-0001-6152-6637
- first_name: KJ
full_name: Lu, KJ
last_name: Lu
- first_name: Y
full_name: Zhang, Y
last_name: Zhang
- first_name: YL
full_name: Cheng, YL
last_name: Cheng
- first_name: SL
full_name: Tu, SL
last_name: Tu
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Tang H, Lu K, Zhang Y, Cheng Y, Tu S, Friml J. Divergence of trafficking and
polarization mechanisms for PIN auxin transporters during land plant evolution.
Plant Communications. 2024;5(1). doi:10.1016/j.xplc.2023.100669
apa: Tang, H., Lu, K., Zhang, Y., Cheng, Y., Tu, S., & Friml, J. (2024). Divergence
of trafficking and polarization mechanisms for PIN auxin transporters during land
plant evolution. Plant Communications. Elsevier. https://doi.org/10.1016/j.xplc.2023.100669
chicago: Tang, Han, KJ Lu, Y Zhang, YL Cheng, SL Tu, and Jiří Friml. “Divergence
of Trafficking and Polarization Mechanisms for PIN Auxin Transporters during Land
Plant Evolution.” Plant Communications. Elsevier, 2024. https://doi.org/10.1016/j.xplc.2023.100669.
ieee: H. Tang, K. Lu, Y. Zhang, Y. Cheng, S. Tu, and J. Friml, “Divergence of trafficking
and polarization mechanisms for PIN auxin transporters during land plant evolution,”
Plant Communications, vol. 5, no. 1. Elsevier, 2024.
ista: Tang H, Lu K, Zhang Y, Cheng Y, Tu S, Friml J. 2024. Divergence of trafficking
and polarization mechanisms for PIN auxin transporters during land plant evolution.
Plant Communications. 5(1), 100669.
mla: Tang, Han, et al. “Divergence of Trafficking and Polarization Mechanisms for
PIN Auxin Transporters during Land Plant Evolution.” Plant Communications,
vol. 5, no. 1, 100669, Elsevier, 2024, doi:10.1016/j.xplc.2023.100669.
short: H. Tang, K. Lu, Y. Zhang, Y. Cheng, S. Tu, J. Friml, Plant Communications
5 (2024).
date_created: 2023-09-01T11:32:02Z
date_published: 2024-01-08T00:00:00Z
date_updated: 2024-01-30T13:00:47Z
day: '08'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.xplc.2023.100669
ec_funded: 1
external_id:
pmid:
- '37528584'
file:
- access_level: open_access
checksum: edbc44c6d4a394d2bf70f92fdbb08f0a
content_type: application/pdf
creator: dernst
date_created: 2024-01-30T12:59:57Z
date_updated: 2024-01-30T12:59:57Z
file_id: '14911'
file_name: 2023_PlantCommunications_Tang.pdf
file_size: 2825565
relation: main_file
success: 1
file_date_updated: 2024-01-30T12:59:57Z
has_accepted_license: '1'
intvolume: ' 5'
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Plant Communications
publication_identifier:
issn:
- 2590-3462
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Divergence of trafficking and polarization mechanisms for PIN auxin transporters
during land plant evolution
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 5
year: '2024'
...