TY - GEN AU - Schlögl, Alois AU - Kiss, Janos AU - Elefante, Stefano ID - 12901 T2 - AHPC19 - Austrian HPC Meeting 2019 TI - Is Debian suitable for running an HPC Cluster? ER - TY - JOUR AB - The inner nuclear membrane (INM) is a subdomain of the endoplasmic reticulum (ER) that is gated by the nuclear pore complex. It is unknown whether proteins of the INM and ER are degraded through shared or distinct pathways in mammalian cells. We applied dynamic proteomics to profile protein half-lives and report that INM and ER residents turn over at similar rates, indicating that the INM’s unique topology is not a barrier to turnover. Using a microscopy approach, we observed that the proteasome can degrade INM proteins in situ. However, we also uncovered evidence for selective, vesicular transport-mediated turnover of a single INM protein, emerin, that is potentiated by ER stress. Emerin is rapidly cleared from the INM by a mechanism that requires emerin’s LEM domain to mediate vesicular trafficking to lysosomes. This work demonstrates that the INM can be dynamically remodeled in response to environmental inputs. AU - Buchwalter, Abigail AU - Schulte, Roberta AU - Tsai, Hsiao AU - Capitanio, Juliana AU - HETZER, Martin W ID - 11060 JF - eLife KW - General Immunology and Microbiology KW - General Biochemistry KW - Genetics and Molecular Biology KW - General Medicine KW - General Neuroscience SN - 2050-084X TI - Selective clearance of the inner nuclear membrane protein emerin by vesicular transport during ER stress VL - 8 ER - TY - GEN AB - The inner nuclear membrane (INM) is a subdomain of the endoplasmic reticulum (ER) that is gated by the nuclear pore complex. It is unknown whether proteins of the INM and ER are degraded through shared or distinct pathways in mammalian cells. We applied dynamic proteomics to profile protein half-lives and report that INM and ER residents turn over at similar rates, indicating that the INM’s unique topology is not a barrier to turnover. Using a microscopy approach, we observed that the proteasome can degrade INM proteins in situ. However, we also uncovered evidence for selective, vesicular transport-mediated turnover of a single INM protein, emerin, that is potentiated by ER stress. Emerin is rapidly cleared from the INM by a mechanism that requires emerin’s LEM domain to mediate vesicular trafficking to lysosomes. This work demonstrates that the INM can be dynamically remodeled in response to environmental inputs. AU - Buchwalter, Abigail AU - Schulte, Roberta AU - Tsai, Hsiao AU - Capitanio, Juliana AU - HETZER, Martin W ID - 13079 TI - Data from: Selective clearance of the inner nuclear membrane protein emerin by vesicular transport during ER stress ER - TY - CONF AB - When can a polyomino piece of paper be folded into a unit cube? Prior work studied tree-like polyominoes, but polyominoes with holes remain an intriguing open problem. We present sufficient conditions for a polyomino with hole(s) to fold into a cube, and conditions under which cube folding is impossible. In particular, we show that all but five special simple holes guarantee foldability. AU - Aichholzer, Oswin AU - Akitaya, Hugo A AU - Cheung, Kenneth C AU - Demaine, Erik D AU - Demaine, Martin L AU - Fekete, Sandor P AU - Kleist, Linda AU - Kostitsyna, Irina AU - Löffler, Maarten AU - Masárová, Zuzana AU - Mundilova, Klara AU - Schmidt, Christiane ID - 6989 T2 - Proceedings of the 31st Canadian Conference on Computational Geometry TI - Folding polyominoes with holes into a cube ER - TY - JOUR AB - The capacity to respond or adapt to environmental changes is an intrinsic property of living systems that comprise highly-connected subcomponents communicating through chemical networks. The development of responsive synthetic systems is a relatively new research area that covers different disciplines, among which nanochemistry brings conceptually new demonstrations. Especially attractive are ligand-protected gold nanoparticles, which have been extensively used over the last decade as building blocks in constructing superlattices or dynamic aggregates, under the effect of an applied stimulus. To reflect the importance of surface chemistry and nanoparticle core composition in the dynamic self-assembly of nanoparticles, we provide here an overview of various available stimuli, as tools for synthetic chemists to exploit. Along with this task, the review starts with the use of chemical stimuli such as solvent, pH, gases, metal ions or biomolecules. It then focuses on physical stimuli: temperature, magnetic and electric fields, as well as light. To reflect on the increasing complexity of current architectures, we discuss systems that are responsive to more than one stimulus, to finally encourage further research by proposing future challenges. AU - Grzelczak, Marek AU - Liz-Marzán, Luis M. AU - Klajn, Rafal ID - 13372 IS - 5 JF - Chemical Society Reviews KW - General Chemistry SN - 0306-0012 TI - Stimuli-responsive self-assembly of nanoparticles VL - 48 ER -